LOCUS       DASSFQ010000001         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_148992, whole genome shotgun sequence.
ACCESSION   DASSFQ010000001 DASSFQ010000000
VERSION     DASSFQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148992"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFH96_00005"
     CDS             complement(<1..521)
                     /locus_tag="VFH96_00005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6972367.1"
                     /translation="MEETTIRECTTIEQFDRCVALQREAFGLPDLELSPRRHLIVSRQ
                     AGGWTLGAFVADRMVGFVHHLAAVRGLEIFGYSHVMAVAKDYQNKGVGARLKWAQRER
                     ALGEGRKLIKWTWDPMLARNAHFNLNRLGATVESYLDDFYGIDYGADEQLGLPSDRLS
                     ATWKIDSARVQALG"
     gene            complement(531..1334)
                     /locus_tag="VFH96_00010"
     CDS             complement(531..1334)
                     /locus_tag="VFH96_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET6972368.1"
                     /translation="MANGIATSAATEPSSREELLIAELRKRTAELEEANRELRRVSHY
                     RSLFLARMSHELRTPLTSILGFTEILLDQEELTDTQRRFCQKVQDSGLQLQASLNQLV
                     DLSRIEAGHTEVFLQEFSIRETLRESCAAAARMAQKQQVKIEYELAPEITTIVTDQGK
                     LRQVLFNFIAWAVSRSPAGESVRINIEGDADGMLLFKVIDNGETRADLPNVFDPEHGS
                     ANHETNINELGIIIGRKLLELINGRVTLENLVPRGLQATIQIPARPLKG"
     gene            1538..3043
                     /locus_tag="VFH96_00015"
     CDS             1538..3043
                     /locus_tag="VFH96_00015"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET6972369.1"
                     /translation="MNGQLSEQPLAELIREISEKSLSGRLRLEHERVHAVAYFDQGKF
                     LYAAANVRTLRVREYLKKAEAVSDQDLAQFNERVSDADLLKVLCAQKLLSVASAEQIQ
                     TRLVADVLRLALLWTEGAWEFDGRSRLSEELDPQIDFDALLLEAARRLPAKFAASRFD
                     NPAELITPVEAPVFNDNLLPAEGFLLSRVDRPTTVREIVAISGLGHDETLGHLYALAL
                     AGLLKREQWPAMFRGEQATRPAPKKITLPPPPPVEREQTHDTDPQDVENLLVRIKNAR
                     THYEVLGVAREISAPELKTVYYQLARRYHPDRFRKSAPELVPRIEAAFARITQAYDTL
                     RDDQLRASYDAKLEVRKKVDQIADAAPKASTPAPQPEAVAEGSPEPVVSAAERAALQF
                     KEGFAAFEMGQKKVALGLFASAARAVPGEPRYRAFYGQVLAENETTRRAAEAELNAAI
                     RLDPNNAEYRMMLAELYRELGLKLRAKGEAERAVAADPNNRKARDLLRSLK"
     gene            3152..>3532
                     /locus_tag="VFH96_00020"
     CDS             3152..>3532
                     /locus_tag="VFH96_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6972370.1"
                     /translation="MAAVQTELIDSAAAEAFIQLAKTTDKFERYENSHAQRIAAIADE
                     IAQQFQLARHDRGSLYAASLLHDLGEVAMERDYIQATGPLSTEERLDLSRHPVLGERE
                     ASRVGADRAAQLLIRWHHEWWNGAG"
BASE COUNT          775 a         1002 c          980 g          775 t
ORIGIN      
        1 ccgagcgcct gcactcgcgc ggaatctatc ttccaggtcg cagacaatcg atcactcggc
       61 agaccgagct gctcatcggc tccgtaatca atgccgtaaa aatcatcaag gtacgattca
      121 acagtcgcgc cgagccggtt gagattgaag tgcgcattac gcgcgagcat cggatcccag
      181 gtccacttga tcaacttccg gccttcgccg agtgcgcgct cgcgctgcgc ccacttcaaa
      241 cgcgcgccga cgcctttgtt ctgatagtcc ttcgcgaccg ccatcacgtg cgagtatcca
      301 aagatctcta acccgcgtac ggccgcgaga tgatgaacaa aaccaaccat gcgatcggcg
      361 acgaatgcgc caagagtcca gccgccggcc tggcgagaca cgatcagatg ccggcgaggt
      421 gagagttcaa gatcgggaag gccgaatgct tcacgctgca acgcgacaca gcgatcaaac
      481 tgttcgatcg ttgtgcactc ccgaattgtc gtctcttcca tccgtaaaca ttatcccttc
      541 aatggacgtg ccggtatttg aattgttgcc tgtaatccgc gtggtacgag attctccaga
      601 gtcacgcgtc cgttgatcag ctcgagcagt ttacgaccaa tgatgatccc gagctcgttg
      661 atgttcgtct cgtgatttgc cgagccgtgc tccggatcga aaacgttagg gaggtccgct
      721 cgtgtctcac cgttgtcgat cactttgaac aacaacatcc cgtctgcgtc accttcgatg
      781 ttgattcgca ctgattcgcc cgccgggcta cgactcaccg cccacgcgat gaagttgaag
      841 agcacctgtc gcaactttcc ctggtccgta acgatggttg tgatctcagg cgcgagctca
      901 tactcgatct tcacttgctg cttctgcgcc attcgcgctg ccgccgcaca cgattctcgc
      961 agagtctctc tgatagaaaa ctcctgtaga aagacttccg tatgccctgc ttcgatgcgc
     1021 gagagatcga caagctgatt caaactcgcc tgcagttgca agcctgagtc ctgcactttc
     1081 tggcaaaagc ggcgctgcgt gtcagtcaac tcttcctgat cgagcaggat ttcagtgaat
     1141 ccgagtatcg aagtcaacgg agtccgcagc tcgtgcgaca tacgcgccag aaacaacgag
     1201 cggtaatgtg aaacccgccg cagctcgcga ttcgcctctt ccagctcggc cgtgcgtttt
     1261 ctcaactcag cgattagtag ttcttcgcgt gagctcggtt ccgtggccgc gctcgtggct
     1321 atcccgtttg ccatcgtaac gttctccctg tcgacacttt cgtgtgctat gtcgtccata
     1381 tagatctcat ccacctgctt gaaaagttca gcaaacttgg gggtcaatca gggtgtttat
     1441 attggcttca atatagggtt taccgttcta ggctgtcaaa gtttagttat atctttcttg
     1501 tgctttttct gcgtctttgt ggctaatata tcgcgggatg aacggccaac tcagcgagca
     1561 gccgctggca gaactgattc gtgaaatctc ggagaagtct ctgagcggca gattgcggct
     1621 cgagcacgag cgcgtccatg cggtcgctta ttttgaccag gggaagtttc tttatgccgc
     1681 ggcgaacgtg cgcacactgc gcgtgcgtga atatttgaag aaggccgaag ctgtttcgga
     1741 tcaggatctc gcgcagttta acgagcgtgt ttccgacgca gatctactca aggtgctgtg
     1801 cgcgcagaag ctgctgtcag tggcttcggc ggaacagatt caaacgcgcc tcgtggctga
     1861 tgtgctgcgg ctcgcgcttt tgtggactga gggtgcgtgg gagtttgacg ggcgatcccg
     1921 tctcagcgaa gaactcgacc cccagatcga ttttgacgcg ctgttacttg aagccgcccg
     1981 caggctcccg gcaaagtttg cggcttctcg tttcgacaat cccgccgaac tgatcacacc
     2041 agtcgaagcg cccgtcttta atgacaacct tttaccggcg gaaggctttt tactttcgcg
     2101 cgtggataga ccaactaccg tgcgtgagat cgttgccatc agtggattgg gccacgatga
     2161 gacactcgga catctttatg cactcgcact cgccggcctg ctcaaacgcg aacagtggcc
     2221 agcgatgttt cgaggtgaac aagcaactcg gccggcgccg aagaagatca cgcttccacc
     2281 tccgccacca gttgagcgcg aacagactca cgacaccgat ccccaggacg tcgagaatct
     2341 tctcgtgcga atcaaaaatg cgcgcacgca ttacgaggtg ctgggtgttg cgcgtgagat
     2401 ctcagcgcct gagctgaaga cggtttatta ccaactggcg cggcgttatc atccggatcg
     2461 ctttcgcaag agcgcgcccg agctagtgcc acgcatcgaa gcagctttcg cgcgaatcac
     2521 acaggcttac gatacgctgc gcgacgatca gctacgtgca agctacgacg cgaaactcga
     2581 ggtgcgcaaa aaagtcgatc agatcgcaga cgccgcgcca aaagcaagca caccggcgcc
     2641 gcaaccggag gcagttgcag aaggcagtcc tgaaccagtc gtatcggcgg ccgaacgggc
     2701 agcgcttcag tttaaagaag gattcgccgc attcgagatg gggcaaaaga aagtcgcgct
     2761 cggattattt gcgtcggccg cgagagctgt gccaggcgag cctcgttacc gcgcttttta
     2821 tggtcaggtg cttgcggaaa atgagaccac gcggcgcgcg gccgaagcag aattgaacgc
     2881 tgctatacga ctggatccga acaacgctga ataccggatg atgctcgcgg agttgtatcg
     2941 agagctcggt ttgaagctgc gggcgaaagg ggaggctgag cgcgctgtcg ccgccgaccc
     3001 aaacaaccgc aaggcacgtg acctcctgcg atctctaaaa tgagccacaa aaaagacgca
     3061 aaaggcacaa ataagaatag ttaagctacg aagttagcct tcgacggttt ctctttttgc
     3121 gatttttgtg cttttttgtg gctaacaaat tatggcggca gttcagacag agttgatcga
     3181 tagcgcggcg gctgaggctt ttatacagct cgcaaagacg acggataagt ttgagcgcta
     3241 cgagaattcg cacgcgcaac gcatcgcagc catcgcggat gagatcgcgc agcaatttca
     3301 actcgcgcga cacgaccgcg gctcgttgta cgctgcgtca ctcttgcacg acctcggcga
     3361 ggtggcgatg gagcgcgatt acatccaggc caccggacca ctttccacgg aagagcgact
     3421 cgatctttcg cggcaccctg tgctgggcga acgcgaggca tccagggtcg gagcagatcg
     3481 cgcggcgcag ctactaattc gctggcatca tgaatggtgg aacggcgcgg gc
//
LOCUS       DASSFQ010000002         4482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1192251, whole genome shotgun sequence.
ACCESSION   DASSFQ010000002 DASSFQ010000000
VERSION     DASSFQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192251"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4482)
                     /locus_tag="VFH96_00025"
     CDS             complement(<1..>4482)
                     /locus_tag="VFH96_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET6972371.1"
                     /translation="VEQAVTLKQVICGGESLPNDLVNLFYERLPQATLHHSYGPTETA
                     IASSETICERNSTYPVTPIGRPLANTQLYVLDQQQQPVPIGVIGELYIGGSGLGRGYL
                     NQPALTATKFVPDLFSTEPGRRLYRTGDMVRYLANGNLEFHGRRDEQVKLRGVRIELG
                     EIENVIRAQEGVRAAIVMMRGDALVAYVVAEVGLNITSSELRHAVAQQLPVSMVPAAF
                     ISLAEIPLLPNGKIDRAALPEPESEATEAYVAPRDEVETLIAGVWADVLVRERVSVHD
                     DFFALGGDSLTATQVMARLFDVFHVDLGLRSLFEASTVAGLAESVSTAVRGGTFSQPL
                     ALLPRTRPEVLPLSFAQQRLWFLEQMESAGAYHIPVIFRIRGLLDVPVLERVIDEIAR
                     RHESLRTTFDVVAGEPVQVISPPRHVPLPIINLSELPEDQREAEAVLLSSEQAQLQFD
                     LTTGPLLRARLVRLSEAEHVLQVTLHHLIADGWSVGVLARETRALYEAFIQDDASPLP
                     ELPAQYADFALWQRDWLKGELLESQVAYWREQLAGAPTALELPTDHMRPPVQSFAGGE
                     IKFEFPASLTEALHKLSRREGATIFMTLLTAFSVLLSRYAGEEDVVVGTPIANRRQSE
                     LEELIGFFANTLALRVDVQPELTFRELLRNVRETTLNAYANQDVPFERLVEELQPPRE
                     LNRSPLFQVLLDVQNTTHSKLVLPELEIGIQEFVTNSTRFDLECFLKEEDGRMLGHVI
                     YSSDLFERQTIEKMLRHFRRLLELVIEDPKQQVRDLRFMTPAEDHQQLVEWNNTKADY
                     PRDKCVHELFEEQVARTPSNIAIEFGDHRLSYSELNRQAGEIAARLQRVGVGPNTIVG
                     ICLERSPAMLIALLGVLKAGGAYLPLDPAYPKARLAYMLDDSGSPVLITHTALRDRFS
                     VLPECVIYVDEHHSLGSEISSVKVTPDDLLYVIYTSGSTGTPKGVAMPHRSLVNLITW
                     QIARSGRPVPRTLQFASFSFDVSFQETFSTWCAGGTLILIDEEVRRDVERLWTTLARK
                     GIERLFLPCVALQGLAEVCIRNKSVVPRLREVVSGGEQLKITPQIRELFAKLNSCLFD
                     NHYGPTETHAMTAYRLAKDETSWPDIGPIGRAISNTQMYVLDPQLKPGPVGVIGEVYL
                     SGDSLSLGYLNRPEATAEKYIPNPFSETPGARMYRTGDLARLLSDGNVEFFGRRDGQL
                     KIRGYRVEIGEIESVLAGHPSINEAIVVAKTGESGAVRLVAYARVGEALAWTELRPYL
                     KERLPEYMIPASFVTVEKFPLTPSGKIDRRGLSLLAEHSVEEELPTEALSPTAELLAG
                     IWGEVLEVEHVNARDNFFDLSGHSLLATRVISRIREAFNLELPLRRLFEYPTVLELAA
                     HIDEQLRVGHASALPPIRPQPHELTRPLSFAQQRIWYWEQLQPGTPTYNLTSALRLEG
                     DLDLGVFERSLNEVVRRHETLRTSFTAIDGQPVQVVAPKKTFRVSFTDLSSMPSMER"
BASE COUNT          976 a         1302 c         1229 g          975 t
ORIGIN      
        1 cgcgctccat cgaaggcatt gagctgagat ccgtaaagct aacccggaaa gttttctttg
       61 gcgccactac ctgaaccggt tgtccgtcaa tcgctgtaaa tgaagtgcgc agcgtttcgt
      121 gacggcgcac gacttcgttg agactccgct cgaagactcc gagatcgaga tctccttcga
      181 gccgcagtgc ggacgtgagg ttgtaagttg gagtgcctgg ttgcaactgt tcccagtacc
      241 agatccgctg ctgcgcaaaa gagagtggcc gcgtgagctc gtgcggttgc ggccggattg
      301 gagggagggc ggatgcgtgg cccaccctga gctgctcgtc gatgtgtgcg gccagctcga
      361 gtacagtcgg atattcaaac agccgccgca atggcagctc taaattgaac gcttcgcgta
      421 tgcgtgaaat gacgcgcgtc gcaagcaatg aatgaccgct gagatcaaaa aagttgtcgc
      481 gtgcgttgac gtgctcgact tccagcacct cgccccaaat gccggcaagc aactctgccg
      541 tgggactcag cgcttctgtt ggtaactctt cttcgacgct gtgttcagcc agcaaagaca
      601 agcctcgacg atcgatcttt ccgctcggcg tgaggggaaa cttctcaacc gttacgaacg
      661 acgccggtat catgtattca ggcagacgct ccttgagata cggccgcaac tccgtccagg
      721 ccagcgcctc acccactcgc gcatacgcaa ctagacgaac ggcgcctgac tctcccgtct
      781 ttgcgaccac aatagcttca ttgattgacg ggtgtccggc gagcacagat tcgatctcgc
      841 caatctcaac gcggtagccg cggatcttca actgaccgtc gcgtcgcccg aagaactcaa
      901 catttccatc actcaacaga cgcgcaaggt cacccgtgcg atacatgcgc gcgcccggag
      961 tttcgctgaa aggatttggt atgtactttt cagccgttgc ttccggacga ttcagatagc
     1021 ccagcgagag actatcaccg ctcaggtaga cctcgccgat tactccgaca ggaccaggct
     1081 tgagttgcgg atcgagcaca tacatctgtg tgtttgagat ggcgcgcccg atcggaccga
     1141 tatcgggcca actggtctcg tccttcgcca ggcgataagc cgtcatcgcg tgagtttcag
     1201 ttggaccata atgattatcg aacaggcagc tgttgagctt cgcaaacaat tctcgtattt
     1261 gcggcgtgat cttgagctgc tcgccgccgc tcactacctc gcgaagcctt ggcactactg
     1321 acttgttgcg tatgcagact tccgccagac cctggagcgc cacacacggc aggaacaatc
     1381 tttcgattcc cttccttgct aacgtggtcc acagacgctc gacatctcgc cgcacttctt
     1441 cgtcaatcag gatgagcgtg cccccggcgc accacgtcga aaaggtttcc tgaaaagaaa
     1501 cgtcgaaact gaacgatgcg aattgcaacg tgcgcggcac tggcctgcca gagcgcgcta
     1561 tctgccacgt aatcaggtta actaacgaac ggtgcggcat cgcgacacct ttcggcgttc
     1621 cggtggatcc agaggtgtag atcacgtaga gcaggtcgtc aggcgtgacc ttaacgctcg
     1681 agatttcgct accgagcgaa tgatgttcat caacgtaaat cacgcattca ggcaaaacgg
     1741 agaaacgatc gcgcaaggct gtatgtgtaa tcaaaacagg agagccggaa tcgtccagca
     1801 tgtaagcgag cctggccttc gggtaggcgg ggtcaagcgg caggtatgcg cctcctgcct
     1861 tcaacacacc aaggagcgct atcagcatcg cgggagaacg ttccaaacaa attcccacga
     1921 tcgtgtttgg tccaacaccg actctttgca aacgcgcggc aatctcgccg gcctgccggt
     1981 tcaattcgga gtagctcaag cggtggtcgc cgaattcgat cgcgatgttt gacggcgtgc
     2041 gcgcgacttg ctcttcaaac aactcgtgta cgcacttgtc gcgcggataa tctgccttcg
     2101 tgttgttcca ctcaaccagt tgctggtgat cttctgcagg cgtcataaat cgcagatcgc
     2161 gaacctgctg ttttggatct tcgatcacga gctccagcag tcgccggaag tgccgaagca
     2221 tcttctcgat tgtttgtcgc tcgaagaggt ctgagctgta gataacgtgt ccgagcatcc
     2281 tgccatcttc ctctttcagg aagcattcaa gatcgaacct cgtcgagttc gtaacaaatt
     2341 cctgtatccc gatttcgagt tccggcagaa cgagtttgct gtgagtcgtg ttctgaacat
     2401 caagtagtac ctgaaatagc ggactgcgat tcaactcgcg cggtggttgc agctcttcga
     2461 cgagtcgttc gaacggcacg tcctggttcg cgtacgcgtt gagggttgtt tcgcgtacgt
     2521 tgcgaagcaa ctcgcggaac gtcagctcgg gctgcacgtc gactcgcaat gcaagcgtgt
     2581 tggcgaagaa tccgatcagt tcctccaact cgctttgtcg ccggtttgca atcggagtac
     2641 cgactacaac gtcttcctca ccggcgtaac gcgaaagcag taccgagaac gccgtcaaca
     2701 atgtcatgaa gatcgtcgcg ccttcgcgcc ggctcaactt gtgcaacgct tcggttagtg
     2761 aggctggaaa ttcgaatttg atttcgccgc ccgcgaagct ttgtactggc ggtcgcatgt
     2821 gatctgtggg cagctcgagc gccgtgggtg cgccggcgag ttgctcacgc cagtaagcga
     2881 cttgcgattc gagcaattcg cccttgagcc agtcacgttg ccaaagtgcg aagtcggcat
     2941 actgcgccgg cagttcaggt aaaggcgaag catcgtcctg aatgaatgct tcatagagcg
     3001 cgcgagtttc acgcgcgagc acgccgaccg accagccatc agctatcaag tgatgcaacg
     3061 tgacttgcag cacatgctct gcttcgctca atcgcaccag acgcgcgcgc aggagtggtc
     3121 cggttgtcag atcaaactgt agttgggcct gttcgctcga gagcaacaca gcctctgctt
     3181 ctctctggtc ttccggcagc tcgcttaaat tgatgatcgg cagcggcacg tgccgtggcg
     3241 gggagatcac ctgcaccggc tctcccgcaa cgacgtcaaa agtagtgcgc agagattcgt
     3301 gacgacgcgc aatctcatct atcactcgtt cgagcacggg cacatcgagc aggccacgaa
     3361 tgcggaagat aaccggaatg tggtaagccc cggctgattc catttgttcg aggaaccaga
     3421 gtcgctgctg tgcgaacgaa agcggcagca cttcgggccg tgtgcgcggc agcagcgcca
     3481 acggttgact gaatgttcct ccgcgcactg cagtgcttac actctccgcc agcccggcga
     3541 cagtcgaggc ttcaaacaag ctacgcaaac cgagatcgac atgaaatacg tcgaacaatc
     3601 gtgccatcac ttgcgtggct gttaacgaat ctccgccgag tgcaaagaag tcgtcgtgta
     3661 cgctcacgcg ctcgcgcacg agcacgtcag cccatactcc tgcaatcagc gtttccactt
     3721 catcgcgcgg ggcgacgtac gcttcagttg cttcagactc gggctccggc agagccgcgc
     3781 gatcgatttt gccgtttggc agcaaaggaa tttcggcgag tgaaataaac gctgccggca
     3841 ccatggaaac cggtaactgc tgtgcgactg cgtgacgcag ctctgaagac gttatattca
     3901 aaccaacttc cgcaacgaca tacgccacga gggcatcgcc gcgcatcatc acgattgcgg
     3961 cgcgcacgcc ttcctgtgct ctgatcacgt tttcgatctc acccagctcg atcctgactc
     4021 cgcgcagctt cacctgttcg tcgcgccggc catgaaactc gaggttgccg ttcgcgagat
     4081 agcgaaccat gtcaccagtg cgatacaacc tgcgacccgg ctcagtgcta aatagatccg
     4141 ggacaaactt cgtcgcagtt aacgctggtt ggttgagata accgcggccc aatcccgagc
     4201 cgccaatgta caactcgccg atcacgccga tgggaaccgg ctgttgctgc tgatccaaca
     4261 catacagttg cgtgttggct agcgggcggc cgatgggtgt caccgggtaa gtactgtttc
     4321 gctcgcagat cgtctctgaa gaagcgatag cagtttcagt tggtccatac gagtgatgca
     4381 gagtcgcttg aggcaagcgc tcgtaaaaga ggtttactaa atcattcggt aacgattcgc
     4441 caccgcaaat cacctgcttc agcgttacgg cctgttccac gt
//
LOCUS       DASSFQ010000003         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1790439, whole genome shotgun sequence.
ACCESSION   DASSFQ010000003 DASSFQ010000000
VERSION     DASSFQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790439"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFH96_00030"
     CDS             <1..397
                     /locus_tag="VFH96_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HET6972372.1"
                     /translation="FTVSACRQDAERGRVPSEVQEVITSVGENIAQERYDQIYSESSD
                     LWKKDVTLDQSNEVFKTLRTKLGKVESRALHSAAEQQNSGGVLKGHVYILSYQTRFER
                     GDGMETFTLIEENGRWRLARYFVNSTALQ"
     gene            406..1701
                     /gene="purB"
                     /locus_tag="VFH96_00035"
     CDS             406..1701
                     /gene="purB"
                     /locus_tag="VFH96_00035"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET6972373.1"
                     /translation="MIDRYTLPEMGALWSEQNKFQKWLDVEIAVCEVHAEMGTIPRDA
                     LEQIKARAKFSVERINEIEKTTNHDVIAFTTNLAESIGEASRFVHYGLTSSDVVDTAN
                     ALLLRDAADILLKKVEQLIETLKRRALEFKNTPQVGRTHGIHAEPTSFGMTFALWFDE
                     MRRNRERLLRAHEAVAVGKISGAVGAFAHLDPTVEEQVCARLGLKAAPVSTQVIQRDI
                     YAEYLTTLAIIASSLDKFALNVRHWQRTEVREAQERFTKGQKGSSAMPHKRNPIISER
                     ICGISRVIRANSIVGLENVALWHERDISHSSAERVVLPDSSIALDYILQKAIGLIDGL
                     VVYPERMLENLRATRGLIFSGQLLLALTNKGLSRETAYEWVQRNAMRVWDEDRDFQEL
                     VKADDDIKSQLSVAEIERIFSLDHYLRNVDAIYGRLGLL"
     gene            complement(1708..2142)
                     /locus_tag="VFH96_00040"
     CDS             complement(1708..2142)
                     /locus_tag="VFH96_00040"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET6972374.1"
                     /translation="MQNPPPVQVSSQPPAQAGKSSTGLDENIAALLSYVFGWVSGLIF
                     FLIEKDSRLVRFHAMQSLLFNVLAAVIAIALWIVLFVVFLIVSQVSGALATVLSLVSV
                     LVWLVLGVAILAGWVLCLVKAFQGQYFKLPVIGNFAEKFSAK"
     gene            2314..2559
                     /gene="purS"
                     /locus_tag="VFH96_00045"
     CDS             2314..2559
                     /gene="purS"
                     /locus_tag="VFH96_00045"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET6972375.1"
                     /translation="MKAKVYVNLKSGVLDPQGKAIQHSVELLGFSGISDVRQGKYFEI
                     SLDGSMDNESALESIEKMAREVLSNPIIEDYRVEIEA"
     gene            2556..3254
                     /gene="purQ"
                     /locus_tag="VFH96_00050"
     CDS             2556..3254
                     /gene="purQ"
                     /locus_tag="VFH96_00050"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET6972376.1"
                     /translation="MKFGVIVFPGSNCDHDAYHVISKHVGQPVDFIWHRETDLSTYDA
                     VILPGGFSYGDYLRAGALARFSPVMNSVKKFAQSGRFVLGICNGFQILCEAGLLPGAL
                     IRNRDLHFICEHIYVRVETPDSPYTNELTRGTVLRIPIAHAEGNYICDDNTLAELQRE
                     DRIVFRYCEKDGTVTDAANPNGSRDNIAGICNRERNVMGLMPHPERACEDLLGSSDGR
                     DIFRSLAATVAAAV"
     gene            3268..3990
                     /locus_tag="VFH96_00055"
     CDS             3268..3990
                     /locus_tag="VFH96_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6972377.1"
                     /translation="MKRILPFIIILVVLAVALGSAWYLTRTIPASPTLGSQSASSSPS
                     SQGSPVAQAQQQTPPVAQGVPGADPPHVLGPADAPIKIEEFGDFECPPCGIFHPILEQ
                     MHQEFGDKMQITFREFPLVPTHQHALAAASAAEAAGLQGKFWEMHDLIYDHQNDWKKE
                     FDVRPIFEGYAKQIGLDVERYKRDMNGELVAQRIFADGKRGHSLGVKGTPTVFMNGRE
                     VPFENLPAEKLRVVIQNELRGK"
     gene            3987..4394
                     /locus_tag="VFH96_00060"
     CDS             3987..4394
                     /locus_tag="VFH96_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET6972378.1"
                     /translation="MKKLYVVAALLALVGLADALYLTIEHVTGQSVRCTIISGCSAVL
                     SSPYAVVAGIPLAAVGAAAYFTVFSLAILTLFNYPHVATLLRAIVITMFALSVWLMYL
                     QAFVIREFCQYCLLSALITTTLLVVTLIARHAR"
     gene            4554..4988
                     /locus_tag="VFH96_00065"
     CDS             4554..4988
                     /locus_tag="VFH96_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972379.1"
                     /translation="MAQQQPTVADIFRRAQSLRQEHSDWSYAQVKDQLVKDFSDGQFP
                     PLHNLTIPEQDARAPEEDWTAGLPIVRRGIQFQDWKEVMTGIIISLEQTENYERERGP
                     EGSRDDWHDRTVGIDDAVAKGVGKWMPEELIALAERKGKKSV"
     gene            5006..>5199
                     /locus_tag="VFH96_00070"
     CDS             5006..>5199
                     /locus_tag="VFH96_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972380.1"
                     /translation="MSFLNNLFQDPKYQTTDPRKPEEIDDQEIIISPDTRREKRIPPG
                     QSRTKKWPVLDAHGTPDIDLA"
BASE COUNT         1222 a         1400 c         1469 g         1108 t
ORIGIN      
        1 tttcacagtg tcggcgtgcc ggcaggacgc ggagcgcggg cgtgtgccga gcgaagtgca
       61 ggaagtgatc acgagtgtcg gtgagaacat tgctcaggaa cgttacgatc agatctacag
      121 cgagtcgtcg gatctttgga agaaagacgt cacgctcgac cagtcaaacg aagtgttcaa
      181 gactttgcga acaaagctcg gtaaagtcga atcgcgcgcg cttcattccg ccgccgagca
      241 gcagaattcg ggtggcgtgc tgaaaggtca cgtctatatc ctgagttacc aaacgcgctt
      301 cgaacgcggc gatgggatgg agacttttac attaatagaa gagaatgggc ggtggcgcct
      361 ggcgcgttac tttgtaaatt ccaccgcgct tcagtgaaga aataaatgat cgatcgttac
      421 accttgcccg aaatgggcgc gctctggtcc gagcaaaaca agtttcagaa atggctcgac
      481 gtggaaatcg ctgtctgtga ggtccacgcc gagatgggca cgattccgcg cgacgcgttg
      541 gagcagatca aagcgcgcgc gaaattttcc gttgagcgca tcaacgagat cgagaagaca
      601 acgaatcacg atgtgatcgc gtttacaaca aacctcgctg agtcgatcgg tgaagcgagc
      661 cggtttgttc actacggtct cacgtcgtca gacgttgttg acactgccaa cgcgttgctg
      721 ctgcgtgacg ctgccgacat tctactcaag aaagtcgaac agttaatcga gacgctgaaa
      781 cgccgcgcgc tcgagtttaa gaacacgccg caagtcggac gcacgcacgg cattcacgct
      841 gagccgacgt cgtttggaat gacgttcgcg ctctggttcg acgagatgcg acgcaatcgc
      901 gaacgcttgc tacgtgcaca cgaagcagtt gcggtgggaa agatcagtgg tgcagtcggc
      961 gcctttgccc atctcgatcc aacggttgaa gagcaagttt gcgcgcgact cggattgaaa
     1021 gcggcgccgg tctcgacgca ggtgatccag cgcgacatct acgcggagta cttaaccacg
     1081 ctcgcgatca tcgcctcgtc gctcgataag tttgccctca atgttcgtca ctggcagcgc
     1141 accgaagttc gcgaggcgca ggagcgtttc actaaaggtc agaagggatc gagcgcgatg
     1201 ccgcacaagc gcaatccgat catctcggag cgcatctgtg gtatctcgcg cgtgatcagg
     1261 gcgaatagca ttgttgggct cgagaatgtg gcgttgtggc acgagcgcga catttcacat
     1321 tcgtcagcgg agcgcgtcgt gttgcctgat tcgagcatcg cgctcgatta cattctgcaa
     1381 aaggcgatcg gattgattga cggcctcgtg gtctaccccg agcgaatgct cgaaaatctt
     1441 cgcgcgacca ggggtttgat ttttagtggt caactgctgc tggcgcttac taataaagga
     1501 ctgtcgcgcg aaacagcgta cgagtgggtg caacgcaatg cgatgagagt ttgggacgag
     1561 gatcgggatt ttcaggagct ggtgaaagcg gacgacgaca tcaaatcaca gctatcagtg
     1621 gcagagatcg agcggatctt ctcgctcgat cactacctgc gaaatgtcga tgcgatctat
     1681 ggaaggctcg ggctgttata ggaccgatta cttcgccgag aacttctcgg caaaattccc
     1741 gatcaccggc agcttgaaat attggccctg aaacgccttg acgaggcaca gcacccaccc
     1801 ggccaggatg gcaacgccca aaactaacca aaccagtact gacaccaaac ttagcactgt
     1861 cgccaaagca ccggaaacct ggctgacaat taagaagaca acaaacagca cgatccagag
     1921 cgcaatcgcg atgacggcag ccagcacatt gaacagcaac gattgcatcg catgaaaccg
     1981 aaccaggcga ctgtcttttt caatcaggaa gaaaatcagc ccggacaccc agccgaaaac
     2041 atatgaaagc aacgcggcaa tattttcatc aagcccggta ctggatttgc cggcttgggc
     2101 aggcggctgg ctgctcactt ggacgggcgg agggttttgc attataagtt tctcctgtta
     2161 gaagggtttt tgactccagg cacgtcaggt cttacgagcc ggagatatta tgccaacaag
     2221 tgctttttaa gcaatgattt gtttgatcca gacggtgatc taaattgaac tgcttctctg
     2281 tgcaacagag aaatctcgag cggcaggtaa cagatgaaag ccaaagttta tgtaaacttg
     2341 aaatccggag tactcgatcc gcagggaaaa gccatccagc actcggttga gctactcggc
     2401 ttctcgggaa tttccgacgt gcggcagggg aaatacttcg agatctcgct cgacggatcg
     2461 atggacaacg agagcgcact cgagtcgatc gagaaaatgg cgcgcgaagt cctttccaat
     2521 cctataatcg aagactaccg cgtggagata gaagcgtgaa attcggagtt attgtttttc
     2581 ccggatcgaa ttgcgaccac gacgcctacc acgtcatctc aaagcacgtg ggtcagccgg
     2641 tcgacttcat ctggcaccgc gaaacagatc tttcgactta cgacgcggtg atccttcccg
     2701 gcggtttttc gtacggcgac tatctacggg ccggcgcgct cgcccgtttc tcgcccgtga
     2761 tgaactcggt caaaaagttt gcgcaaagcg gtcgcttcgt gctcggcatc tgcaacggtt
     2821 ttcagattct ctgtgaagcc ggtctgctgc cgggcgctct gatccgcaat cgcgatctgc
     2881 acttcatctg cgaacacatt tacgttcgcg tcgaaacgcc tgacagtcct tatactaacg
     2941 agctgacgcg cggcacggtg ctgcgcattc cgatcgcgca cgccgaagga aactatatct
     3001 gcgacgacaa cacgctcgcc gagctgcaac gagaagaccg cattgttttt cgttactgcg
     3061 aaaaagatgg gaccgtcacg gacgccgcaa atccaaacgg ctcgcgtgac aacatcgccg
     3121 gaatttgtaa tcgcgagcgc aatgttatgg gtttaatgcc gcatccggaa cgcgcgtgtg
     3181 aagatttgct cggctcgagt gacgggcgcg atattttccg ctcacttgcg gctacggtgg
     3241 ccgcagctgt ttgattcttc aattactatg aagcgaatac tacctttcat catcattctc
     3301 gtcgttctcg ccgtcgcgct cggcagtgcc tggtacttaa cccggaccat cccggcctcg
     3361 ccgacacttg gaagccaatc cgcttcgtca tcaccgagct cgcagggttc accggtggca
     3421 caagcacagc agcagacgcc gccggtagct cagggcgttc cgggagcaga tccgccacac
     3481 gtgcttggac cagcggatgc gcctatcaag atcgaagagt ttggtgactt cgagtgtccg
     3541 ccatgcggga tcttccaccc gatactcgaa cagatgcatc aggagtttgg cgataagatg
     3601 cagatcacgt ttcgtgaatt tccactcgtg ccgacgcacc agcacgcgct cgcggctgcg
     3661 tcagccgctg aagccgccgg gctacagggc aagttttggg aaatgcacga tctgatctac
     3721 gaccatcaga acgactggaa aaaagagttt gacgtgcgcc cgatcttcga aggctacgcg
     3781 aaacagatcg ggctcgatgt tgagcgctac aaacgcgaca tgaacggcga gcttgtggcg
     3841 caacgaatct ttgcggacgg caaacgcggg cattcactcg gcgtgaaggg tacgccgacc
     3901 gtgttcatga atggccgcga agtgccattc gagaatcttc ctgctgaaaa gctgcgcgtt
     3961 gtgattcaga acgagctgcg gggtaaatga agaagcttta cgtcgtcgcc gcgctgcttg
     4021 ccctggtcgg acttgccgat gcgctttacc tcacgatcga acatgttacg ggacaaagtg
     4081 tccgctgcac gatcatctcg ggctgctcgg ctgttttgag tagtccatac gccgtggtag
     4141 cgggcattcc gctcgctgcg gttggcgccg cggcgtattt cacggtcttc agtctcgcca
     4201 ttctcacgct cttcaattac ccgcatgtcg cgacgctttt gcgagcgata gtgatcacga
     4261 tgttcgcgct cagtgtctgg ttgatgtatc tgcaggcctt cgtcattcgc gagttttgcc
     4321 agtattgcct cctctccgcc ctgatcacga ccacgctctt agtcgtaacg ctgattgctc
     4381 gtcacgcaag ataatcacga acgggtggcc acggagggcc acccctacag ttgtatttga
     4441 tcgatggaga acaactgtag gggcggccct ccgtggccgc ccgtcgtttg gtgcctgatg
     4501 ctgcgtcagt gtaaggtatt ggtttccaaa tcagactaag agagcaggtg acgatggctc
     4561 agcagcaacc cacggtggcg gacatttttc gtcgcgcgca gtctttgcga caagagcatt
     4621 cggactggtc gtacgcgcaa gtgaaagacc agttggtcaa agatttttcc gacgggcaat
     4681 ttccaccact gcacaacctg accatccccg agcaggacgc gcgcgcgccc gaggaagact
     4741 ggactgccgg actcccgatc gtgcgacgcg gtattcagtt tcaggactgg aaagaagtca
     4801 tgaccgggat cattatcagt ctcgaacaaa ctgaaaacta cgaacgcgag cgcggccccg
     4861 aaggatcgcg cgacgactgg cacgatagga ctgtcggcat tgacgatgcg gtcgcgaagg
     4921 gcgtcggcaa atggatgccg gaagagttga tcgcgctggc ggagcgcaaa ggcaagaaat
     4981 ctgtgtaatc tgtggatgag tattcatgag ctttttaaat aatctatttc aggatccgaa
     5041 ataccagaca accgatccgc gtaaaccgga agagatcgac gatcaggaga tcatcattag
     5101 tcccgacacg cggcgcgaga aacgcattcc gccaggacag tcgcgcacca agaaatggcc
     5161 cgtgctcgat gctcacggca cgcctgatat cgatctcgc
//
LOCUS       DASSFQ010000004         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1046569, whole genome shotgun sequence.
ACCESSION   DASSFQ010000004 DASSFQ010000000
VERSION     DASSFQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046569"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..741
                     /locus_tag="VFH96_00075"
     CDS             148..741
                     /locus_tag="VFH96_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6972381.1"
                     /translation="MSRENILFAIIGLLLGFIVGFMFASSMAQKNAMQPATAGALPAD
                     HPPVGAQGQNAPNPQAMQAQVQASLEKARSEPQNFDAQMQAADLYYQIQRYDQALEYL
                     LKANSLKPTDYKTVVLLGMVNLDAQHYDQAEKWYRAAMKMKSDDVMVLAGLAATTLQK
                     GDAKAAADAIAQLEKVDPNSEDLPQFRDKLASLKAGK"
     gene            complement(814..1764)
                     /locus_tag="VFH96_00080"
     CDS             complement(814..1764)
                     /locus_tag="VFH96_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972382.1"
                     /translation="MAKLFAVAILLLAVACAAAQSIDPNAPSPVRSNSVTGRIAARDL
                     GDARLTDHYYALTGTPGDLLITVNSTNLNGDIDVFTATTLRPLLKLTLYAESAAPVTK
                     GIYLRKREELILRIEARSPNDDEGTYRLSFGGSFEPIVGGPEIAEAETPATETTTAGG
                     RKTKRVSSVGARIEEPEPPVTEVATTTPTPEPTPEATPEAKPVETPAAKPEKSPEVEK
                     PATVRNTRGRRPIRRPAPRVKESPPPVATVAEPKPKPEPEPEIGPRLVIETNDGTLIN
                     RSMSTVRRVTVENGQVVVTGKDGKIDRILLVNIVRMSIAP"
     gene            1807..>2628
                     /locus_tag="VFH96_00085"
     CDS             1807..>2628
                     /locus_tag="VFH96_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6972383.1"
                     /translation="MKLIRSVGLPVLFVLLSSLPAHAQTWTAKLDKDINFYQPTEMGL
                     LIVGTEKSLYAIDASTGDTLWRRKDSSLDETDVATVPGTDLLLLSFEKGDKARIEAVD
                     ILTGDAIWRSEKIKGAVMQTTVDTANNLLAVVVAKDAKSRARDGFKRRPLLHVLDLAT
                     GEELWKYDISEVEMMPARWPEDSGKEVDYTLDNYYPPAFIDGRLYVFYEGVTSFDARN
                     GKERLREKYRVNEDGLALTEAEPIFTEGMIYVSGHGRVRAISRQSGDTEWEAKDLG"
BASE COUNT          585 a          722 c          750 g          571 t
ORIGIN      
        1 ttccaggcga aagattacaa gaaggcaatc gactactggc aacgtgtgct gaagaaagtt
       61 ccgccgggtt cggaagtcgc ggaaacgatt actgcacgca tcaacgaagc aaagacactc
      121 gcggcgagta aataaaccag agagaacatg tcgagagaga acattctttt cgcaattatc
      181 ggactgcttt taggcttcat cgtaggcttc atgtttgcca gctcgatggc ccagaagaat
      241 gcgatgcaac ccgccaccgc gggcgcgctt ccggccgatc atccaccagt cggggcgcag
      301 ggccagaacg cgccgaaccc gcaggcgatg caggcgcagg ttcaggcttc gctcgagaag
      361 gcgcgcagcg agccgcaaaa tttcgacgcg cagatgcagg cggctgatct ctactaccag
      421 attcagcgtt acgatcaggc gctcgagtat ttgctgaaag cgaactcgct taagccgact
      481 gactataaga ctgtcgtgtt gctcggcatg gtgaatctcg atgcgcaaca ctacgaccag
      541 gctgagaagt ggtatcgagc cgcgatgaag atgaagtcag atgatgtaat ggtgctcgcc
      601 ggcctcgccg ccaccactct gcaaaaaggc gacgcgaaag ccgcagcgga cgcaatcgcg
      661 cagctagaga aagtcgatcc taattcagaa gatcttcctc aattccggga taaattagct
      721 agtttgaagg caggaaaata gccacaaaag gcacaaaaag cacaaaagag ttcaataaac
      781 attttgtgct ttttgtgcct tttgtggcta tctttaagga gcgatcgaca ttctcacgat
      841 attcactagc agtatgcgat cgatcttgcc gtccttgccg gtcacgacca cctgaccatt
      901 ctcaaccgtc acacgcctaa cagtactcat cgatcgattg ataagcgttc cgtcattggt
      961 ttcgatcacg agccgtggtc caatttccgg ttcgggttca ggttttggct tcggttctgc
     1021 aacggtggca accggcggtg ggctttcttt aactcgcggt gcgggacggc gaataggtcg
     1081 gcggcctcgc gtattgcgta cggtcgcggg tttctcgact tcgggagact tctcaggttt
     1141 cgctgccggc gtctcaaccg gcttcgcttc cggcgttgct tctggtgtcg gctcgggtgt
     1201 cggcgttgta gtcgctactt cagtgactgg cggctcaggt tcttctatcc gtgcgccgac
     1261 agacgacacg cgcttcgtct tgcgtccacc tgcagtcgtt gtttcggtag ccggtgtttc
     1321 agcttccgcg atctcaggcc caccgacgat cggctcaaac gatccaccga aggacaaccg
     1381 gtaagttccc tcatcatcgt ttgggctgcg cgcttcgatg cgcaggatca gttcctcgcg
     1441 cttgcgcagg tagattccct tcgtgacggg agccgcgctc tcggcgtaca gcgtgagttt
     1501 gagcaacggt cgcaacgtag tcgcagtgaa cacgtcgatg tcgccgttga gattcgtgct
     1561 gttgacggtt atcaacaggt cgcccggcgt cccggtcaat gcgtaataat gatccgtcaa
     1621 tcgtgcatca ccgagatcgc gcgcggcgat gcggcccgta acgctgttgc tgcgtacggg
     1681 agacggcgcg ttcggatcga tcgactgcgc agcagcgcat gcgactgcca ggagtaaaat
     1741 ggcgacggcg aataacttgg ccataattcg ccgcaaagca tatcagattt gcgttatcct
     1801 agccgcatga agttaatccg ctcggtcggg cttccggtgc tctttgtttt gctctcttcc
     1861 cttcccgctc acgcccaaac gtggacagca aaactcgata aagacatcaa cttctaccag
     1921 ccaactgaaa tgggtctgct catcgtcggc actgaaaaat cgctctacgc gatcgacgcc
     1981 tcaaccggcg acacgctctg gcgtcgcaaa gacagttcgc tcgacgaaac cgacgtcgca
     2041 actgttcccg gcaccgatct gttgttgctg agttttgaga aaggcgacaa agcacgtatc
     2101 gaagccgtcg acattctgac aggcgacgcg atctggcgta gcgaaaagat caaaggcgcc
     2161 gtgatgcaga cgaccgtcga cactgcaaac aatctgctcg cggtcgtggt tgcgaaggat
     2221 gcgaagagcc gtgcgcgcga tggtttcaag cgtcgcccat tgttgcacgt tctcgatctg
     2281 gccactggcg aagagctctg gaagtacgac atcagcgagg tcgagatgat gccggcgcgc
     2341 tggccggaag actccggcaa agaagtcgat tacacgctcg ataactatta cccgccggcg
     2401 tttattgacg ggcggttgta tgttttttac gaaggcgtta cttcattcga tgcgcgcaac
     2461 ggcaaagagc gactgcgcga gaagtaccgc gtcaatgaag acggactcgc gctcaccgaa
     2521 gccgagccga tctttaccga gggcatgatt tacgtttccg gtcacgggcg cgtgcgagcg
     2581 atctcgcgtc agagtggcga cacggagtgg gaagcaaaag atctcggg
//
LOCUS       DASSFQ010000005         2885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1642612, whole genome shotgun sequence.
ACCESSION   DASSFQ010000005 DASSFQ010000000
VERSION     DASSFQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642612"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFH96_00090"
     CDS             complement(<1..919)
                     /locus_tag="VFH96_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET6972384.1"
                     /translation="MLLSIDVGTSGVRAALYDERGNEVPGARAHSRRPISISNFGELY
                     PNRLVNEVVDTIDQLPFNTPIDHIAISTFWHSLLGVDSAGHPTTKLLTWADTRAAQFA
                     NELRSQFNEAQIHHRTGCRFHPSYWPAKLRWLEHEEKDHYNRTHRWLGFAEFLCLRLF
                     GEIATFVSTASATGLFNQRNCDWDWEFVDALGLVRETLPDIKTQTDARLTGEFASRWP
                     ALAEARLVTVVGDGAANNIGAGCSTKDKIALMVGTSGAMRVVFEGGPPDHLDPGLWCY
                     RANRERVVVGGALSDGGGVVQWLYESLDLY"
     gene            complement(929..1957)
                     /locus_tag="VFH96_00095"
     CDS             complement(929..1957)
                     /locus_tag="VFH96_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HET6972385.1"
                     /translation="MSKRSFASDNNAGVHPEIIDAIRNANDGHVLAYGNDPVTARAME
                     LFQKHFGSDVAVYFVFGGTGANVLGLKAITQSHHAIFCADSAHVHVDECGAPEKFTGC
                     KLVTIPTPDGKLRAELIKPLLHGIGVEHHVQPRVISISQATEMGTVYTRDELKALAAF
                     AHDNGLLLHVDGARLANAAVALDKTFKEITTDVGVDVLSFGGTKNGMMYGEAVVFFDK
                     TRAGDFKYIRKQGMHLPSKMRFISAQFEALLSGDLWRRGAEHANRMAQVLGSELAQTP
                     QIKLTQPVESNGVFATIPKEYIPTLQDKYFFYVWDDAISEVRLMASFDTSEDDVHDFV
                     AFVRETVK"
     gene            complement(1993..>2885)
                     /locus_tag="VFH96_00100"
     CDS             complement(1993..>2885)
                     /locus_tag="VFH96_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6972386.1"
                     /translation="SASYPKGGMGGFSHALANAAKAAGVEIRTSARVSQISGAYGDKP
                     KVVLEGGEEIESRVIVSNADPRTTFLNLVDPIDLEPNFLLKMRNYRAPGVVAKINLAL
                     DGLPSFRGVDDDNTKLSGRIHIGPEIDYLERAFDAAKYGEFSSDPYLDISIPSLGDPS
                     LAPAGKHVMSIHAQFAPYKLRDGDWTTHGEKFANHVISKLEEYAPKINEMIVAGQVIT
                     PHDLEETYGVSGGHIHHGEQTLDQFFTFRPLIGFAQYRTPLKRLYLCGAGTHPGGGLT
                     GLPGANAAREIARDFKEKKV"
BASE COUNT          625 a          838 c          785 g          637 t
ORIGIN      
        1 cgtacaggtc caatgattca tacagccact ggaccacgcc gccaccatct gacaatgcac
       61 caccaacaac tacacgctcg cggttcgctc gataacacca caaacccgga tcgagatggt
      121 ccggtggtcc accttcaaac acgacacgca tcgcaccaga cgtgccgacc atcaacgcga
      181 tcttgtcttt cgtgctacaa cccgcgccga tgttgtttgc ggcgccgtcg cctacaaccg
      241 tgacgagtcg cgcttcagcg agcgcgggcc aacgtgatgc aaattcaccg gtgagccgcg
      301 catctgtttg cgttttgata tcgggtaggg tctcgcgaac gaggccgagc gcgtcaacga
      361 attcccagtc ccagtcgcaa tttcgttgat tgaataagcc ggtggctgaa gctgtcgaga
      421 caaacgttgc gatttcgcca aagagtcgca ggcaaagaaa ttctgcgaag ccaagccacc
      481 ggtgcgttct gttgtagtgg tccttttcct cgtgctccag ccagcgtagt tttgcgggcc
      541 agtaactcgg atggaagcga caacccgtgc ggtggtgaat ttgcgcttcg ttgaactgcg
      601 agcgcaattc gtttgcgaat tgagcggcgc gcgtgtcggc ccaggtaagc agcttcgtgg
      661 tgggatgtcc ggcggagtcg acgccgagta gactgtgcca aaacgtagag atggcgatgt
      721 gatcgatcgg cgtgttgaat ggtaattgat cgatcgtatc tacaacttcg ttgacaaggc
      781 gattggggta gagctcgcca aaatttgaga tcgagatagg gcgacggctg tgggcccgcg
      841 cgcctggcac ttcattgccg cgctcgtcat acaacgctgc gcgcacacct gacgtaccga
      901 cgtcaatgct cagtaacatg tttttgagtt acttgactgt ctctcgcaca aacgccacga
      961 agtcgtgaac atcatcctca ctcgtatcaa acgacgccat caatctcact tccgagatcg
     1021 cgtcgtccca aacgtagaag aaatatttgt cctgaagagt gggaatgtat tccttcggga
     1081 tcgtcgcaaa cacaccattc gactcgactg gctgcgtgag tttgatctgc ggcgtctgcg
     1141 ccagctcgct accgagcacc tgcgccatcc ggttcgcatg ctcggctccg cgacgccaca
     1201 gatcacccga gagcagcgct tcaaactgcg ctgagatgaa tcgcatcttc gatggcaggt
     1261 gcatgccctg cttgcgtatg tacttaaaat ctcccgcgcg cgttttatcg aaaaacacaa
     1321 cggcctcgcc gtacatcatg ccgttcttcg tgccgccgaa cgaaagcaca tcgacgccaa
     1381 catccgtcgt gatctctttg aacgtcttat cgagggcgac cgccgcattc gcgagtcggg
     1441 caccatcgac gtgcaacagc agcccgttgt catgagcaaa ggcagcaagg gccttcaatt
     1501 cgtcgcgcgt gtaaaccgtg cccatctcag ttgcctgcga gatcgagatg acgcggggct
     1561 gcacgtgatg ctcgacaccg atgccatgca gcagcggctt gattagctct gcgcggagtt
     1621 taccgtccgg cgtgggaatc gtaactaact tgcagccagt gaacttctca ggcgcgccac
     1681 actcgtcaac gtgaacgtgc gcactgtcgg cacaaaagat cgcgtgatgc gattgagtaa
     1741 tcgccttcaa accgaggacg ttcgcgcccg tgccgccaaa gacaaaataa accgcgacgt
     1801 cgctgccgaa gtgtttttga aacaactcca tcgcgcgcgc ggttacgggg tcgttgccat
     1861 aagcgagcac gtggccgtcg ttcgcgttcc tgatggcatc gatgatctcc ggatgcacac
     1921 cggcgttgtt gtcgctcgcg aagctgcgtt tactcataac caatccgttc aaaaatccgt
     1981 gttatccgtg actcaaactt tcttctcctt aaaatctcgc gcgatctcac gagcggcgtt
     2041 cgcgcccggc aaacctgtta agccaccgcc cggatgcgtg cccgcgccgc acaggtaaag
     2101 tcgcttgagt ggcgtacgat actgcgcgaa accaatcaac gggcgaaaag tgaagaactg
     2161 atcgagagtt tgttcgccgt ggtgaatatg tccaccgctc acaccgtacg tttcttcgag
     2221 atcgtgcggt gtgatcacct ggccggccac aatcatctcg ttgatcttcg gcgcgtactc
     2281 ttcgagtttg ctaatgacgt ggttcgcaaa cttttcgcca tgcgtagtcc agtcgccatc
     2341 tctcaacttg tacggcgcga attgagcgtg gatcgacatc acgtgtttgc ccgcaggcgc
     2401 cagcgatgga tcgccaagcg aaggaatgct gatatcgaga tagggatcgc ttgaaaactc
     2461 gccgtacttc gctgcatcga acgcacgctc gagatagtcg atctcggggc cgatgtgtat
     2521 ccgaccagag agtttcgtgt tgtcatcatc tacgccgcgg aatgacggca aaccatcgag
     2581 cgcgagattg atctttgcca caactccggg cgcgcgatag ttgcgcatct tcaaaagaaa
     2641 gttaggttcg agatcgatcg gatcgacgag attcagaaac gtcgtccgcg gatcggcgtt
     2701 ggaaacaatc acgcgcgatt caatctcctc gccgccttcg agcacgactt tcggtttgtc
     2761 gccgtatgcg ccgctgatct gggaaactcg cgctgacgtg cgtatctcca ctcctgcggc
     2821 tttggctgcg tttgcgagcg cgtgactaaa accgcccatg ccgcctttcg gatacgacgc
     2881 ggatc
//
LOCUS       DASSFQ010000006        26714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1643637, whole genome shotgun sequence.
ACCESSION   DASSFQ010000006 DASSFQ010000000
VERSION     DASSFQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 26714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26714
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643637"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VFH96_00105"
     CDS             <1..1433
                     /locus_tag="VFH96_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972387.1"
                     /translation="MLFLESKTLNLAVHTAGRIRLLQELHAQNLVPLIRSQRLRCFED
                     VLLGIDRVNEMETSLASSTESTTGEPYVRGDLFCFQDYILFLVFEDDDSLHAGIVYEA
                     RTPEPFRKLDAFCHDVRGILLSQQQQIAISSPEWETRGAAMARGFRTFVERQDADSLF
                     TSLRKETMSKRILAASKLEDESARVFLRTARNAHQEGYAAKLLTGHTESVDVPIQRLE
                     DAGLVEREVQISCRKTGHALFRLPNPHALAVVTVSDATCSECGAPVADENVEEVIAPT
                     QLASSLLEDGSWLVSRIHFLLREMGVPERDIALGPAEGNGYGQLMANICGESFLIVTR
                     DGDLTPALARWAIDLEIDTEASHLMIVATGRIHKEAGVLLQNHSRRRVSGGQDFEMII
                     ADDVATAGQELEQALERVSQRVVAEQMCVLDSSLGFSVSRLVLTKFQLMRAAQPKEPE
                     VIVESDEPAQEFVPSAPLSLAAAHEA"
     gene            1505..2083
                     /locus_tag="VFH96_00110"
     CDS             1505..2083
                     /locus_tag="VFH96_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972388.1"
                     /translation="MKQAKKSPARRTDDERWARDIVTLRQAAQDLAHGRHKPQIGPFD
                     QIAGLLDDPSPAVREKAVRDLYAINPDQAATLVNDALRDGSPEERRRIGSALADSGLL
                     YEAIDDLMAENHENCYSAFSLLFLVAKAGVVEPLSSIIEKHPSLDLSLAVIRLLASSG
                     EPEVTSTLQRLASNNALPPELRAAAAESISRF"
     gene            2144..4129
                     /gene="uvrB"
                     /locus_tag="VFH96_00115"
     CDS             2144..4129
                     /gene="uvrB"
                     /locus_tag="VFH96_00115"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET6972389.1"
                     /translation="MFKLQSEYQPKGDQPVAIDQLVKGLNDGAKDQVLLGITGSGKTF
                     TIASVIQRVQRSTLVLAHNKTLAAQLFQEFRSLFPQNAVEYFVSYYDYYQPEAYVPAA
                     DVYIEKEAIINEEIDRLRMSATRALFERRDVIVVASVSCIYGLGDPDAYYGLLFFVEP
                     GERMSRDVLLQRLVELQYERSDINFQRGTFRVRGDVVEIYPSYQDQAYRIELWGDEID
                     SVSTIDPLLGEVIAKHTSRVPIYPKTHYVMSRPTIKRAIKTIREELEWWEPKLISEGK
                     LVEAQRLHQRTMFDLEMIKEMGFCRGIENYSRHLTGKNPGEPPPTLLDYLPRDTLIVI
                     DESHQSVPQVRGMFEGDQSRKRTLVEYGFRLPSALDNRPLNFAEFESRIGQTIYVSAT
                     PGPYELTKASGEVVEQIIRPTGLMDPEVEVQPVKGQIDHLLEECRQRAERHERVLVTT
                     LTKRMAEDLTEYFTEVGVRVRYLHSDIETLERIRILRDLRRGEFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSEQSLIQTIGRAARNASGKAILYADRVTESMKRAMEETQ
                     RRRAIQEAFNRENGIVPQTIVKPIEATLITASEADYFKVPSEVEEIEDYSPAKLEATI
                     TRLGVEMRAAAKRFEFEKAAELRDRIKVLRDRELQVM"
     gene            4201..5634
                     /locus_tag="VFH96_00120"
     CDS             4201..5634
                     /locus_tag="VFH96_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6972390.1"
                     /translation="MQTKSQPDEIETFLTDASFIRDGYADRVVLPEIIDDVSEILASA
                     NRDRVPVTVSGAGTGTVGGRVAFGGIVLATDKLNHIKSIDRDGFAVVEAGVILADLQR
                     AVDQQGLLYPPDPTERGCFVGGNVATNASGARTFKYGPTRNYIRGLKVVLASGEVIDL
                     RRGEVHAGANGRMRIGKIDLTLPPFHMPATRKNATGYFIAPEMDAIDLFIGSEGTLGV
                     ICEIEVRLLPKPEGLLSGVVFFANEADVLALVADARKHADARAIEFFDNESLNFLREK
                     YPNIPPQAVGAIFFEQETTEATEELVFSEWMSLLDQHHALSDSWFATNEQDQAKLREF
                     RHQLPVLMNEWFAHYKQRKVSTDMAVPDDAFAGLFRLYKDTLSSSGLRYTIFGHIGDN
                     HVHVNILPRNDTEGARARELYVQFLKYAASVGGTLSAEHGVGKLKRDYLHLFYTDDEL
                     RAMATIKKFLDPNGILGRGNIFSEELL"
     gene            5662..6051
                     /locus_tag="VFH96_00125"
     CDS             5662..6051
                     /locus_tag="VFH96_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET6972391.1"
                     /translation="MFTHVNVLTREIIGAAIEVHRHLGPGLLESAYLQCLTRELCLKE
                     IPFKREWPLPLEYKGVRLSCSYRLDLLVADAVVVEIKSVEALTSIHDAQLLTYLKLGG
                     WKVGLLINFNVEVLKTGIHRKVLGLVE"
     gene            6143..7312
                     /gene="aroC"
                     /locus_tag="VFH96_00130"
     CDS             6143..7312
                     /gene="aroC"
                     /locus_tag="VFH96_00130"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET6972392.1"
                     /translation="MLRFTTAGESHGRALVAIVEGLPAGVPVDQELIDKELRRRQLGY
                     GRGGRMKIERDHAEILTGVRHGLTLGSPLTLLIENKDWPNWTDVMASEPRDIAPEKSR
                     RLKRPRPGHADLPGGLKYDVRDLRNILERASARETTARVACGALAKQLLAAFGAEVRS
                     HVIQLGGIPELPLELSWEQMAAIPDDAPLHCADNDAQQRMIDLIDEVRHKGDTLGGVF
                     EVAARGVIPGLGSHTSWDLKLDGLLAQAVMSIPAVKAVAIGAGAEASSLPGSEVHDEI
                     AYNNDTREFIRETNRAGGLEGGITNGEQIRIRGHLKPLSTLRRALRSVDIDTKEEETA
                     AFERSDVTAVPAAGVIGESMVALVLAGAMREKFGGDSLGEMKRNFEGYREQLRGY"
     gene            7376..7882
                     /gene="def"
                     /locus_tag="VFH96_00135"
     CDS             7376..7882
                     /gene="def"
                     /locus_tag="VFH96_00135"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET6972393.1"
                     /translation="MEIVKWPAPVLDTPADPVTEFDDDLKKLVNNMFETMYAAPGVGL
                     AAVQVGVPQRLFVMDCSGGKDPSQRVALINPEVLRVEGNQNGEEGCLSFPGIFTPVER
                     SLRAIVRAQDVKGEWFELDGMELTARCMLHETDHCDGIVFLDKMSALKRELVKRKIKK
                     LQKAGKWD"
     gene            7964..9523
                     /gene="pruA"
                     /locus_tag="VFH96_00140"
     CDS             7964..9523
                     /gene="pruA"
                     /locus_tag="VFH96_00140"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET6972394.1"
                     /translation="MPNEFRNEPFTDFTKEENAQAMRAAIQKVQSQLAREYPLVIGGE
                     RINTGNFLESINPANRTQVVGRFNKATKELANRAVEEAAKAFETWKNTPAADRAALLF
                     RVAAIMRERKHELSAWMIHEVAKSWPEADGDTAEAIDFCEFYAHEMLRYASDQPLVHI
                     PGEQNHLEYIPLGVGAVIPPWNFPLAIMAGMTVASVVTGNTVVLKPSSDAPTIAFKFF
                     EILEEAGLPAGVVNFMTGSGAEVGDVVVDHPKTRYIAFTGSMEIGLRVNERAAKVHKG
                     QIWIKRVVAEMGGKDAIIVADDADLEEAATGVVQSAFGFQGQKCSACSRAIIDSRVYD
                     SMLEKIADRTARIKLADPTDQSTTMSAVINEKAFKAINSYIEKGKSEGGRVLIGGGSD
                     GEQGFFIEPTVIADVKPGATIEQEEIFGPVLAVIKAENYDDALKIANDTQFGLTGAVY
                     SNDEAKLERARREFHVGNLYLNRKCTGALVGVHPFGGFNMSGTDSKAGGRDYLLLFLQ
                     AKVASERVTAD"
     gene            9574..10503
                     /gene="fmt"
                     /locus_tag="VFH96_00145"
     CDS             9574..10503
                     /gene="fmt"
                     /locus_tag="VFH96_00145"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET6972395.1"
                     /translation="MKIVFMGTPQAAVPTLQRCLKDGHEVVAVWTQPDKPAGRGNKVA
                     FTPVKEFALEHGLSVFQPGRIKNDEAKQLFASHDADVAVVVAYGRILPDEFLRAPRRG
                     CINVHFSLLPLYRGAAPANWAVVNGETETGVTTMFIEPTLDTGPILLQHKTPIAEKET
                     APELMQRLAEIGAELLGETLASLDTLTPRPQRHADATFAPVLKKEDGLIDWSLSAFQI
                     ERRIRGFQPWPNAYTKFDSKSLILWEAQPSISVTSGISEGEVIVAQKDELVVQCGQRT
                     ALRVLEVQPEARKRMAARDFINGMHLKVGDRFG"
     gene            10496..11773
                     /gene="rsmB"
                     /locus_tag="VFH96_00150"
     CDS             10496..11773
                     /gene="rsmB"
                     /locus_tag="VFH96_00150"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET6972396.1"
                     /translation="MVKSKEISPARVAAFNILQQVENGAFSSVLLAVQEPNLQPLDRA
                     LCHELVLGVLRWQLFLDKIVEHFSSRRVESLDPAVRIALRLGLYQLRFLTRIPASAAV
                     NESVSLVRAARLSSATAFVNAVLRRAVREPEYDPAAEISDPLEKIAVQTSHPAWLVER
                     WVRSFGIEEAEAFARANNTVPPTAFRVVANRANQSEILSRLSAAGAALESSDIVSGAW
                     RVSGATSLLREMSSAGEIYLQDEASQLVADAMEVKRGERVLDLCAAPGGKTTLIADRA
                     PDAAFIVAADRSATRMATVISTMRLHELKSIKPLILDATEPLPFAPGSFDRVLVDAPC
                     SGTGTLRANPEIRWRLAPADFQNFAEQQKRILSRAVEVLKPGGRLIYSTCSVEKEENE
                     EVIERVDLKVLKTVRTWPQREGTDGFFISILEK"
     gene            11888..12532
                     /locus_tag="VFH96_00155"
     CDS             11888..12532
                     /locus_tag="VFH96_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET6972397.1"
                     /translation="MSVVTRILSVFRSIAIVIAIGIVFLFGVATTVYLSLRSPEVKVP
                     DVVGKNRFDAEKILADADLKFRVRATRPSTEAQPDTVMFQLPRAGEVVKTGQTVAMDV
                     SRLAKAGESSENVTPDEPAENRNTNSAANTNENKPKRPKPKNTNENANANGNTNGNAN
                     KSINANRANVNRVNTNTGAPNTNATPRSNINANTRPAAVNSNRPAPRPTPKPNQ"
     gene            12536..13213
                     /gene="rpe"
                     /locus_tag="VFH96_00160"
     CDS             12536..13213
                     /gene="rpe"
                     /locus_tag="VFH96_00160"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET6972398.1"
                     /translation="MNRDRHIDIAPSILSADFSCLGDQIKAVERGGAGVLHVDVMDGH
                     FVPNITIGLPVVKSLAGFTRLPIDTHLMITDPGTYATQFVKAGASMVSVHVEADAHLH
                     RTLMSIKDAGALAGVVLNPATPVVAVEEALLFADYVLVMSVNPGFGGQKFIPQAVDKV
                     RRLRRMIEERQLNVRIEIDGGIDRKNIETVVAAGAEIIVAGSAIFNTPDAEAAVKELR
                     DATVQWV"
     gene            13520..14929
                     /gene="bamD"
                     /locus_tag="VFH96_00165"
     CDS             13520..14929
                     /gene="bamD"
                     /locus_tag="VFH96_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET6972399.1"
                     /translation="MSSKLDLLRRSLNAAIKALEPKTDKDKKANADDPVAVLKSLEKE
                     VGSVTNEVNDIRNKNEKAEKFDTTALDRLEATVTELNTRVETNLQQTAGLRTTATANN
                     SSGDKKKKKGKLFGLFGGGDDKYADLTGAAVAGRDRVLFEEAAKEVRKGHHDTGRLLF
                     ATIINTYPDSPFLPLAKLAIADSFFLEGTTSALIQAAQAYQDWLTFFPTDPLADDASL
                     KVAEAEMRQMGLSDRDISHARKAEQRLKVLLQQYPKSPLRDTVEARLHEVQENLAMHN
                     LQIARFYYDVKYSTGKGGLKGAQSRLKEITDKYPCFSYNDEVFFRLASTYQAEEEPDE
                     AARYYQKLVQEFPESEYNDKAREQLNIIGAPIPEKSAPNPCSKRTQQSFMGNLMQQVS
                     GKADVTVNKNGILISKDGKEGTDLIDEALRYNGTLPALTTPDAPTQRTVKPAATPAAD
                     SKSKSANGNGGAPAAVVKP"
     gene            14946..15422
                     /locus_tag="VFH96_00170"
     CDS             14946..15422
                     /locus_tag="VFH96_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972400.1"
                     /translation="MEPTSPEAQAELARRHKSAATTVVGLLIATVLLSVVAFLSDAYL
                     VEKPNPPLDIAVRIVILIFGLGAVVWRRTKFQPMRLQDIAGLAGVSGLIKTLEKTTVQ
                     IALIGAAIAAIGFIATLLTGNERYSYWAGAIAVVVFIYSYPTKSSWLKTMYRFTEK"
     gene            15543..16073
                     /locus_tag="VFH96_00175"
     CDS             15543..16073
                     /locus_tag="VFH96_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET6972401.1"
                     /translation="MRLLPITGLLLVFIAIPAFGQEARLITALGPMAVISTPATPVVE
                     PNDIEKRAFEQTNIVRVKNGLRPLTWDADVCRMARIHSESMSRNHYFSHVTPEGKRLP
                     ERARAAGILKFSTLGENIAYSNGYDDPGAYIVEQWMQSSKHRANILSNDFRAMAIGSF
                     VGPDGSIYLTQTFISR"
     gene            16070..16996
                     /gene="ispE"
                     /locus_tag="VFH96_00180"
     CDS             16070..16996
                     /gene="ispE"
                     /locus_tag="VFH96_00180"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET6972402.1"
                     /translation="MITTPAKFPSFAKINWFLRILGKRPDGYHEVVTVLQTISLRDEL
                     TIQLSEDGPFKLTCDDPTIPTDNSNLIIKAAHLLEECRQDWFGASIHLRKRIPAQGGL
                     GGASSNAAVALLAINELWKSSSISVEELLDPSDLGADVPFFLHGGRCAATGTGTDLTP
                     LPDGPKQYLIVITPNARVSTAHAYASLNAASLTTFNSDSILSSSLADLNSADSRQWPL
                     RNDFERVIFEIEPEIERVKNALVDAGAWGALLAGSGSSVFGVFDSEAARDRGLENLRC
                     EAGWRVFPCETISRDEYCRAMNSSGFPLFTLS"
     gene            17021..17095
                     /locus_tag="VFH96_00185"
     tRNA            17021..17095
                     /locus_tag="VFH96_00185"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17053..17055,aa:Gln,seq:ttg)
     gene            17119..18066
                     /locus_tag="VFH96_00190"
     CDS             17119..18066
                     /locus_tag="VFH96_00190"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET6972403.1"
                     /translation="MSTTGGIKIFSGNANPKLAEEICRSLTCDMGKAYPERFSDGEFN
                     FQIDENVRGGDVFIVQPTCPPTDAHLMELLVMLDAFRRSSAERITAVIPYYGYGRSDK
                     KDRPRVPISAKLVANLITVAGADRLLTIDLHAAQIQGFFDIPTDHLYAAPVFIGYYQE
                     HPLPNLTVVAPDTGGAERARAYAKRLNADLALCDKRREKANVAEVMNVVGDVRGRSCL
                     IVDDMCDTGGSLTKVARALKEAGAERIHACFTHPVFSGKAVELLRESDIEKVIVTNTI
                     PLRDHACELDKIKVLSIAPLLAKAIKSIHEETSVSSLFV"
     gene            18168..18833
                     /locus_tag="VFH96_00195"
     CDS             18168..18833
                     /locus_tag="VFH96_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET6972404.1"
                     /translation="MAEKKEYKVQAKVRDGRGKNDARRARREGLVPVTVYGGGAETVA
                     AVAPLRELAAILRSDSGRNTIFTIDVEGVGASEVMFHDRQIDPVKGRLIHADLTRLVK
                     GQKIEVTVPLHLTGEPFGVREKQGVLEQIIREVDIRCEPREIPDTLEVDVTNLDVHET
                     LHVSDIKVAEGVEILTDGEQVIATVGIVKEEVAAAPAEGEEPAEPEVIGKGKKEDEEG
                     DEG"
     gene            18913..19476
                     /gene="pth"
                     /locus_tag="VFH96_00200"
     CDS             18913..19476
                     /gene="pth"
                     /locus_tag="VFH96_00200"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET6972405.1"
                     /translation="MKLIVGLGNPGKEYESTRHNLGFMLIDKLAHDEGISVNSRECSS
                     LVGRGVIEGTRTKLVKPQTFMNLSGHAVACLLKKIESESPIDHLVVISDDLALPVGKI
                     RIRERGSAGGHNGLKSIIASIGTDEFIRLRIGIQPEHPISDSKRFVLDTISRSERAIF
                     EETIAQSADAIRTIIRDGALKAMSIFN"
     gene            19506..19919
                     /gene="rpsF"
                     /locus_tag="VFH96_00205"
     CDS             19506..19919
                     /gene="rpsF"
                     /locus_tag="VFH96_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET6972406.1"
                     /translation="MDTKRQYEVVFIVDPTADDEVPKLTESFSQILTDQGAVVIKAET
                     MGKRQLAYEIQHHKDGTFVLIEMEGSGKEIAELERRMRVNDRVLRYITVRVDEDRRRA
                     EKLKARRARKAAKRPAPGEHRRRGPAMEFAEASGE"
     gene            20055..20264
                     /gene="rpsR"
                     /locus_tag="VFH96_00210"
     CDS             20055..20264
                     /gene="rpsR"
                     /locus_tag="VFH96_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET6972407.1"
                     /translation="MHRRRVCRFCIDKVDLIDFKDVKLLQNYLPERGKILPRRISGNC
                     ATHQRMLAEAIKRARNIALLPYTTD"
     gene            20277..20810
                     /gene="rplI"
                     /locus_tag="VFH96_00215"
     CDS             20277..20810
                     /gene="rplI"
                     /locus_tag="VFH96_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET6972408.1"
                     /translation="MAHTKVLLREDVDDLGARGEIVRVRSGYARNYLLPRNLAVEATA
                     GNVKGIESERAALLKKEAKERSSAEAQSQQMGALELEFKRKAGEQGLLYGSVTSMDVA
                     EALRERGYEIDRHRIHLREPLKRVGEYTVPVRLHREVTIDLKVRVAPEGEVIVGHLSP
                     EQEAELAKTNEEESNAD"
     gene            20905..22281
                     /gene="dnaB"
                     /locus_tag="VFH96_00220"
     CDS             20905..22281
                     /gene="dnaB"
                     /locus_tag="VFH96_00220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET6972409.1"
                     /translation="MATPDPIREQPFERALPNSAEAERAILGAVLLDNGLISQAIEQL
                     RPEDFYVPSHRRVFVAMIALFERGAEINPILIGEELKKESSLEAVGGISFITNLAYGL
                     PHSTNIAHYAKVVRGKSMLRQLIKASNKITQEALAEEDEPELILDHAEHAIFQLADER
                     VRQGFMHVKPIAEQLLEKVQEMEGRAAVLTGLTTGFADLDKMTSGLQNSDLIILAARP
                     SMGKTSFALMLAENAAILSGACVGVFSLEMSKESLVMRMLCAQGNIDAQRFRNGFLSR
                     LEWAQIAKSLGVLADTKIFLDDTPGLSVLEMRAKARRLAAEQKKLDLIIVDYLQLMSG
                     SSKRTESRQQEVSQISRELKGLAKEMNVPLVALSQLSRAPESRSDHRPQLADLRESGA
                     LEQDADVVAFIFREEQYKTAEEREAMPEDRKNIAELIIAKQRNGPTGTVDLRFTPSSM
                     RFDNLYRE"
     gene            22367..24226
                     /locus_tag="VFH96_00225"
     CDS             22367..24226
                     /locus_tag="VFH96_00225"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972410.1"
                     /translation="MKTRKSTLQLVFILALLVCSGSAAFAQALSIKLSVDATQAARNI
                     LHTKFTIPVRPGPLTLFYPKWIPGEHSPTGPINDLVGLRLSAGGKPIAWQRDQVEMFA
                     FHCEVPQGVNELEVSFDDVSQPETTASAKLARIKWNRLILYPRDLNSDTIQVRASLQL
                     PAGWKFASALPMLSENKDGFQFNEVSLTELVDSPAIIGANFRKFALTSTGITHEIDAV
                     ADTPAALVMKPETLTGLKNLVQEAYALFGARHYRSYKFLVTFSDHGGSEGLEHHESSE
                     DGVGEKALVDELELIDFADLMGHEYAHSWNGKYRRPAGLTTPDFEQPMKGELLWVYEG
                     LTEYLGRVLPARSGLWTADDFHEAMAAVAAEFEMQSGRQWRPLVDTAVAVQFTYPSPR
                     AWMNYRRRVDYYDEGSLVWLDADVLIRQRSNGKLSLDDFCRRFHGGSDTSPSVKTYTF
                     DDVVNTLNEVMPYDWRGFLTERIYQVAPHAPLAGITGGGWKLVYTDKPNTLVRLGDHA
                     RKSVDLMYSIGAMLKEDGSVMDVNPTLEAARAGIAPGMKIITVNGRAWSTDALHEAIT
                     AAKSSTTPIELVVENGSFTGVYKLNYHGGERYPHLERDPTKPDMLGEVIKSRR"
     gene            complement(24223..26301)
                     /locus_tag="VFH96_00230"
     CDS             complement(24223..26301)
                     /locus_tag="VFH96_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6972411.1"
                     /translation="MKLKTPITLVIAFLFTFSTITAQTGARWNSVRTNHLFVIGNADP
                     ENLRRVAVWLEYFHAAFARLVSRNVIDSSIPTTVIVFRDDASFTPFKPVYNGRPTSIS
                     GFFLPGDDVNYIAFSLDPSGDPYGTAFHEYVHLHVKDNVPNAPVWLNEGLAELYEALQ
                     FSGGDAIIGMPDHNYLYTLRQAELLPLKTLFSIGLDSPHYNERDKAGIFYGESWALVH
                     YLMFGDRARPDQFKRFLQRIASGDDAAKAIEGTYGTTLDVIEQELQFYVRRGNLTTQR
                     ITGVANPEAYRSYTATQRSALTDSEAYYYLSDLSLHMGRYTVAERGLKEAIAMDPGFL
                     PAYAALGSMYVEQRRYAEAKNYLQKATSSTQSYLVHYYYAYVLSREGISANGEVSQYS
                     RENAAMMREQLLQSVKLAPNYAPAYYLLGVVDFLSDQADEALEMAQKAQQLSPANANY
                     AELVQDIKQYRAGSTNTRGRREAIKGDTIAEPTRTTSSRMLGGDGGPVAINDGQTVDN
                     SGALPAIDEVLDKYVQAIGGAAAVNAVTSRVVKGTVDIVGVSRGGTFETYTVRPNKSV
                     GIIKPAGAEPVVMSGDADFYSIINLKNKYAKFTLVGKSKIGYREVYVIDMKPVSGASD
                     RLFIDAESYLPVRMNTPRAEIYFDDWREADGLKLPHVITFTVQKRTMTLTVNEIKTNV
                     PIDAKIFGKP"
     gene            complement(26425..>26714)
                     /locus_tag="VFH96_00235"
     CDS             complement(26425..>26714)
                     /locus_tag="VFH96_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972412.1"
                     /translation="LGSKAPANWQLNGDVSTWAEQIANGILPTAREAHQRLRFDNIEL
                     DQAQTLVRRGKAFEKKHGPGGQFYAVWAADVVRTQIQKGGWRLAALLEEVL"
BASE COUNT         6145 a         7036 c         7814 g         5719 t
ORIGIN      
        1 cgatgttatt tctcgaaagc aaaacactga atctcgcagt ccacactgcc ggccgcatca
       61 gactgctcca ggaacttcac gcgcaaaacc tcgtgccgct gatccggtca cagcgtttgc
      121 gctgtttcga agacgtcctg ctcggcatcg atcgcgtcaa cgaaatggag acctcgctcg
      181 cgagctcaac tgaatcaacc accggcgagc cgtacgtgcg tggcgacctg ttctgctttc
      241 aggattatat tctcttcctc gtcttcgaag acgatgactc gctccacgcg ggaatcgttt
      301 acgaagcacg cacacccgaa cctttccgaa agctcgacgc gttctgtcac gacgttcgcg
      361 gcatcctgct ttcacagcag caacagatcg cgatatcgtc cccggaatgg gagacgaggg
      421 gcgccgcgat ggcccgcgga ttcagaacgt tcgtcgagcg acaggacgcc gactccctct
      481 tcacgagcct gcgcaaggaa acgatgagca agcgcatcct cgccgcctcg aaactcgaag
      541 acgaaagcgc gcgcgtgttt ctgcgaacgg cgcgcaacgc acaccaggaa ggttatgccg
      601 ccaaactgct caccggccac acggaatccg tagacgtacc gattcaacgg ctcgaagacg
      661 cgggcctcgt tgagagagaa gtccagatca gttgccgcaa gacaggacac gcgctctttc
      721 gcttgccgaa tccacacgcg ctcgcagttg tgacggtgag tgatgcgacg tgcagcgaat
      781 gcggcgcgcc ggtggctgat gagaacgtcg aggaagtaat cgcgccgacg caactggcgt
      841 cgtcgttgct cgaagacgga tcgtggctcg tcagccggat tcattttctg ttgcgcgaaa
      901 tgggcgttcc ggagcgtgat atcgcgcttg gaccagccga aggcaatggt tacggccagt
      961 taatggcgaa tatctgtggc gagtcgtttt tgatcgtgac tcgggatggc gatctgacgc
     1021 cggcgctggc gcgttgggcc atcgatctcg agatcgatac tgaggcttcg cacctcatga
     1081 tcgtcgcgac cgggcgcata cataaagagg ccggagtgtt gctgcaaaat cattcgcgac
     1141 gccgtgtgag cggtggtcag gacttcgaga tgatcattgc tgatgacgta gcgacagccg
     1201 ggcaggagtt ggagcaggcg ctcgagcgcg tgtcgcagcg cgtggtggcg gagcagatgt
     1261 gtgttctgga tagcagcctc gggttctcgg tgagccggct cgtgttgacg aagtttcagt
     1321 tgatgcgggc ggcacaaccg aaggagccgg aagttatcgt ggagagcgat gagcctgcgc
     1381 aagagtttgt gccgagtgcg ccgttaagtt tggcggcggc acatgaagcg taaacagtat
     1441 ttatccacag attacacaga attgttttat gacatctgtg taatctgtgg atgaagaaac
     1501 gttgatgaag caggctaaga aatccccggc acggcgcacc gacgatgagc ggtgggcgcg
     1561 agacatcgtc acactccggc aagccgcaca ggatctcgcc cacggtcggc ataaaccaca
     1621 aatcggacca ttcgatcaaa ttgccggact gcttgacgat ccgtcgcccg cagtgcgtga
     1681 gaaagcagtc cgtgatctgt atgcaatcaa tcccgatcag gccgcaacac tcgtgaacga
     1741 cgcgctccgc gacggctcgc cggaagaacg ccggcgcatc ggcagcgcgc tcgccgattc
     1801 gggtttgttg tatgaagcga tcgacgactt gatggctgaa aatcacgaga actgttacag
     1861 cgcgttttcg ttgttgtttc tcgtcgcgaa ggcgggcgtc gtcgagccgc tcagcagcat
     1921 catcgagaaa catccgagtc tcgatctgag tctagccgta atcagattgc ttgcctcgag
     1981 cggcgaacct gaagtcactt caaccctgca acgcttagct tctaacaacg cactcccacc
     2041 cgaattacgt gccgccgctg ccgaatccat aagccgtttt tgacaggact tacaagattt
     2101 accaagatgt acgggtaaat ccgttttcaa tctgtgtaaa tctgtgttca agcttcaatc
     2161 cgaatatcaa cccaagggtg atcagcctgt cgccatcgat cagctcgtca aaggtctgaa
     2221 cgacggcgcc aaagatcagg tgctgctcgg catcacgggc agcggcaaaa cattcacgat
     2281 cgcgagcgtt atccagcggg tgcaacgctc aacgctcgtg ctcgcgcaca acaagacgct
     2341 cgcggcgcag ctctttcagg agtttcgctc gctctttccg cagaacgccg tcgagtactt
     2401 cgtctcttat tacgactact accaaccgga agcgtacgtt cccgccgccg acgtctacat
     2461 cgagaaggaa gcgatcatca acgaagagat cgatcgcctg cggatgtctg caacgcgggc
     2521 gctgtttgag cgtcgcgacg tgatcgtggt ggcgagtgtc tcgtgcatct acggtctcgg
     2581 cgatccggat gcgtattacg gtctgctttt cttcgtcgag ccgggcgagc gtatgtcgcg
     2641 cgacgtgttg ttgcagcgtt tggtagagct gcaatacgaa cgctcagaca tcaactttca
     2701 gcgcggcacg tttcgtgtac gcggcgacgt cgtcgagatc tatcccagtt accaggacca
     2761 ggcgtaccgg atcgaattgt ggggcgatga gatcgattca gtctcgacga tcgatccgtt
     2821 gctgggcgaa gtgatcgcga agcacacatc gcgcgtgccg atctatccga agactcacta
     2881 cgtgatgtcg cggcccacga tcaaacgagc gatcaagacg attcgtgagg agctcgagtg
     2941 gtgggagccg aagctgatct cggaaggaaa actggtcgag gcacagcgac tacaccagcg
     3001 cacgatgttt gatctcgaga tgatcaagga gatggggttt tgtcgcggta tcgaaaacta
     3061 ttcgcgacac ctgacgggca agaatcccgg cgagccgccg ccgacgctgc tcgactacct
     3121 gccgcgcgac acgctgatcg tcattgacga gtcgcaccag tcggtgccgc aggtgcgtgg
     3181 catgttcgaa ggcgaccagt cgcgcaagcg cacgctcgtc gagtatgggt ttcgtttgcc
     3241 gtcggcgttg gataaccggc cgttgaactt cgcggagttc gaatcgcgca tcgggcagac
     3301 gatctatgtt tcggcgacgc ctggtcctta tgagctgaca aaggcgagtg gtgaagtcgt
     3361 cgagcagatc atcaggccga cgggattgat ggatccggaa gtggaagtgc agccggtcaa
     3421 gggtcagatc gatcatttgc ttgaagagtg ccggcagcgt gcggagcggc acgagcgagt
     3481 gctggtgacg acgttgacca agcgcatggc tgaagatctg acggaatatt tcaccgaagt
     3541 tggtgttcgc gttcggtatt tgcactcgga tatcgagacg ctcgagcgca tcaggattct
     3601 gcgtgacttg cggcgtggtg agtttgatgt gctcgtgggg atcaacttgt tgcgtgaagg
     3661 cttggatctg ccggaggtgt cgctggtcgc aattctcgat gcggataagg aaggttttct
     3721 gcggtcggag cagtcgttga tacagacgat cgggcgtgcg gcgcgcaatg cgagcggcaa
     3781 ggcgatcttg tatgcggatc gtgtgactga gtcgatgaaa cgtgcgatgg aagaaacgca
     3841 gcggagacgg gcgatacagg aggcgttcaa tcgtgagaac gggatcgtac cgcagacgat
     3901 cgtgaagccg attgaagcga ccttgattac ggcgtcggaa gcggactact tcaaggtgcc
     3961 atctgaggtc gaggagattg aggactactc gcccgcgaaa cttgaagcga cgatcacgcg
     4021 gttgggggtc gagatgcgcg ctgcggcgaa gcgcttcgag tttgagaaag ccgccgaatt
     4081 acgagatcgc ataaaggtat tgcgcgatcg tgaattgcag gtcatgtaga gacagaattt
     4141 acaagatttg caggattaca agatgaattc tgtgaatcct gtgaatcctg taaatcctgt
     4201 atgcaaacaa agtctcagcc agacgagatc gagacgtttt taacggacgc cagtttcatc
     4261 cgtgatggtt acgcagatcg tgtcgttctg ccggagatca ttgacgacgt ttccgagatt
     4321 ctcgcgagcg ccaaccgcga tcgagtgccg gttacggttt ccggtgcggg cacagggacg
     4381 gttggcgggc gcgtcgcctt cggtgggatc gtccttgcta ctgacaagct gaatcacatc
     4441 aagagcatag atcgcgatgg ctttgccgtg gttgaagcgg gagttatcct cgctgacctg
     4501 caacgcgcag tggaccagca ggggttactg tatccacccg atccaaccga gcgcggctgc
     4561 ttcgtcggcg gcaatgtcgc caccaacgct tccggcgcgc ggacgttcaa gtatggacca
     4621 acacggaact acattcgagg gctcaaagtc gtgcttgcgt cgggtgaagt gattgatctt
     4681 cgtcgcggtg aagtgcatgc cggtgcgaat gggcgaatgc gtatcggtaa gattgatctg
     4741 acgcttccgc cttttcacat gcctgcgacg cgcaagaatg cgaccggata tttcatcgcg
     4801 ccggagatgg acgcgatcga tctgtttatc ggcagtgaag gcacgctcgg tgtgatctgc
     4861 gagatcgaag tcaggctact cccgaaaccg gaaggtctgc tgagcggtgt cgtgttcttt
     4921 gcgaacgaag cagacgtgct cgcactcgtt gccgatgcac gaaaacatgc cgacgcacgc
     4981 gcaattgagt tttttgacaa cgagtcgttg aacttcctgc gcgagaagta tccaaacatt
     5041 cctccgcaag ccgtcggggc gatctttttc gagcaggaga cgactgaagc gacggaagag
     5101 ttggtcttca gtgaatggat gtcgctcctc gatcagcatc atgcgctatc cgattcgtgg
     5161 tttgcgacga acgagcagga ccaggcaaag ctgcgcgaat ttcgtcatca acttccagtg
     5221 ttgatgaacg aatggtttgc gcactacaag cagcgcaaag tctcgaccga catggccgtt
     5281 cctgacgacg ccttcgcggg gttatttcgc ttatataagg acacgctttc ttcgagcggt
     5341 ctgcgctaca ccatcttcgg ccacatcggc gacaaccacg ttcacgtcaa catcctccca
     5401 cgcaacgaca ccgaaggcgc tcgcgcccgt gagttgtacg ttcaattctt aaagtatgca
     5461 gcctcggtcg gtggcacact ttctgccgaa cacggcgtcg gcaagttgaa acgcgactat
     5521 ctgcatttgt tctacaccga cgatgagctg cgcgcgatgg caacaataaa aaaattcctc
     5581 gatccaaacg gaatcctggg ccgcggaaac attttctctg aagaattgtt ataaattatc
     5641 tttgcgaaag gaacccgtaa catgttcacc catgttaacg tcctcactag ggagatcatc
     5701 ggtgcggcca tcgaggtcca tcgccacctc ggcccaggct tgttggagtc ggcttacttg
     5761 caatgtttga ctcgcgagtt atgtctgaaa gaaataccat ttaagcgcga atggccgttg
     5821 cctctagagt acaagggagt tcgcctgagc tgcagttatc gtctggacct actcgtcgct
     5881 gacgctgtgg ttgttgaaat aaaatctgtt gaggctttga catctattca tgatgcgcag
     5941 ctacttacct atctcaaact tggaggttgg aaagtaggac tgctgattaa tttcaatgtt
     6001 gaagttttga agacagggat tcaccgcaag gtgttaggat tggttgaata actctgcgta
     6061 acctctgcgt tctctgcgcc tctgtggtaa atatcaccgc agaggcgcag agaacgcaga
     6121 ggttacgcag agaagagaat acatgctacg tttcactact gcaggagaat cacacggacg
     6181 agcgctcgtc gcgatcgttg aggggttgcc cgccggagta cccgtcgatc aggaattgat
     6241 cgataaggag ctgcgacgac gacagctcgg atatggtcgg ggcgggcgga tgaagattga
     6301 gcgcgatcat gccgagatac ttaccggtgt ccgccatggg cttacgctcg gctcgccgct
     6361 taccttactg atcgagaaca aagattggcc caactggact gacgtcatgg cgtccgagcc
     6421 gcgcgatatt gcgccggaga agtctagacg tctgaaacgt ccacgacctg gccacgccga
     6481 tctgcccggt ggtctgaaat atgacgtgcg tgatctcagg aacattctcg agcgtgcctc
     6541 cgctcgcgag acaacggcgc gcgttgcgtg tggcgcgctc gcgaaacaac tgttggctgc
     6601 tttcggcgcc gaagtgcgca gtcatgtgat ccagcttggt ggaattcccg agttgccgct
     6661 cgagttgagc tgggaacaga tggccgccat ccctgacgac gcgccgctgc actgcgccga
     6721 caacgacgca caacaaagaa tgatcgatct gattgatgaa gtgcgtcaca agggcgacac
     6781 gctcggcggc gtcttcgaag ttgcggcgcg tggtgtgata cccggtttgg gctcgcacac
     6841 ttcgtgggac ttaaagcttg atggattact cgcgcaggca gtcatgtcga taccggcagt
     6901 gaaggcagtg gcgatcggcg ccggtgcgga agcgagtagt ctgccgggtt ctgaagtaca
     6961 cgacgagatt gcttacaaca acgacacgcg tgagttcatc cgcgagacga accgcgccgg
     7021 cggactggaa ggcggcatca cgaacggcga acagatacgc attcgcggtc atctaaaacc
     7081 gctttctaca ctacgacgcg cgctgcgatc agtcgatatc gatactaaag aagaagagac
     7141 cgcggcgttc gagcgttcgg atgtgacggc tgttccggcg gcgggcgtga ttggtgagtc
     7201 gatggttgcg cttgtgctcg cgggcgcgat gcgggagaag ttcggcgggg atagcctcgg
     7261 cgagatgaag cgcaatttcg aaggatatcg agagcagtta agagggtatt aggagacagg
     7321 attacaggat tttcaagatt aacaggacat agcgtagaat caggtcatgg ctttattgga
     7381 aatagttaag tggccggcgc cggtgctcga tacacccgca gacccggtga ctgagtttga
     7441 cgacgatctc aagaagctcg tcaacaacat gttcgagacg atgtatgcgg cgcccggtgt
     7501 cggactcgcc gccgtgcagg ttggagttcc gcagcgactc ttcgtgatgg actgctctgg
     7561 tggcaaggat ccgtcacagc gcgtggcgct gatcaatccg gaggtgctgc gcgtcgaggg
     7621 aaatcagaat ggcgaggaag gctgcctgtc gtttccgggc atcttcacgc cggtcgagcg
     7681 cagcttgcgt gcgatcgtgc gcgcgcagga tgtaaaaggc gagtggtttg aactcgacgg
     7741 tatggagcta acagcacgct gcatgctgca cgaaaccgat cactgtgacg gcatcgtgtt
     7801 tctcgacaaa atgagcgcct taaaacgcga gctcgtaaag cgcaagatca agaaattaca
     7861 aaaagctgga aagtgggact agcagaatag tccacagatt acgcagatta cgcagaggct
     7921 tatatctgtg taatctgcgt aatctgtgga taggagtatt aatatgccta acgaatttcg
     7981 taacgaacca ttcacagatt tcaccaaaga agagaacgcg caggcgatgc gcgcggcgat
     8041 tcagaaggtc cagagtcagc tcgcacgcga gtatccgctc gtgatcggcg gcgaacgcat
     8101 caacaccggt aactttctcg agagtatcaa tccggcgaat cgcacacagg tcgtcgggcg
     8161 cttcaacaaa gcaaccaaag agctcgcgaa tcgcgccgtt gaagaagccg caaaagcttt
     8221 tgagacgtgg aagaacacgc ctgctgcgga tcgcgctgcg ctgttgtttc gcgtcgctgc
     8281 gatcatgcgc gaacgtaaac atgagctgtc ggcgtggatg atccacgaag tggcgaagtc
     8341 gtggcccgaa gccgacggcg atactgccga agcgatcgac ttttgcgagt tctatgcgca
     8401 cgagatgttg cgttatgcga gcgaccagcc gttggttcac attcccggtg aacagaatca
     8461 tctcgaatat atcccgctgg gcgtcggcgc ggtgattcca ccctggaact tcccccttgc
     8521 gatcatggcc gggatgactg tcgcttccgt cgtcacgggt aacacagtcg ttttgaagcc
     8581 atcgtcggat gcgccaacga tcgcgttcaa gtttttcgaa atactcgaag aggccggttt
     8641 gcctgcgggc gtcgtgaact tcatgacggg ctcgggcgcg gaagtcggcg acgtcgtggt
     8701 ggaccacccg aagacgcgct acatcgcgtt caccggatcg atggagatcg ggctgcgcgt
     8761 caacgagcgc gccgcgaaag ttcataaggg acagatctgg atcaagcgcg tggtggcaga
     8821 gatgggcggc aaagatgcga tcattgttgc cgacgatgct gatctcgaag aggccgcgac
     8881 aggcgtggtg caatcggcgt ttggatttca gggacagaag tgttcggcct gttcacgcgc
     8941 gatcatcgac agccgagttt acgattcgat gctcgagaag atcgccgacc gcaccgccag
     9001 gatcaaactt gccgatccga ccgatcagtc gacgacgatg agcgccgtga tcaatgagaa
     9061 ggcgttcaag gcgatcaact cttacattga aaaaggaaag agtgaaggcg ggcgcgtttt
     9121 gattggcggc ggttccgatg gcgagcaggg gttctttatc gagccgacgg tgatcgcaga
     9181 cgtgaagccg ggcgcgacga tcgaacagga agagatcttt ggaccagtgc tcgcggtcat
     9241 caaggccgag aactacgacg acgcgctcaa gatcgcgaat gatacgcagt ttggtctgac
     9301 gggcgcggtt tacagcaatg acgaagcgaa gctggagcgc gcgcgcagag agtttcatgt
     9361 cgggaatctg tatctgaatc gtaagtgcac tggcgcgctg gttggtgtgc atccgtttgg
     9421 cggcttcaat atgtcgggta ctgattcgaa ggcaggcgga cgcgattacc tgttgttgtt
     9481 tctgcaggcg aaggtggcgt cggaaagagt gaccgcggat tagacggatt ttaaatccgg
     9541 atttgatccg tgtcatccgt ggctaataaa accatgaaaa tcgtattcat gggcacacct
     9601 caggcagctg ttccgacatt gcagcgctgt ctgaaagatg gtcatgaagt tgtggccgtt
     9661 tggacccagc ctgataaacc tgccgggcgc ggtaacaaag tagcctttac tccagtcaaa
     9721 gagtttgccc tcgagcacgg gctgagtgtc tttcaacccg ggcggatcaa gaacgacgaa
     9781 gccaaacaac tcttcgcctc acacgatgct gacgtcgcag tagtcgtcgc gtacggccgc
     9841 atcctgcctg acgagttttt gcgcgcgccg cgtcgtggtt gtatcaacgt tcatttctct
     9901 ttgctgcctc tttaccgtgg cgcggcgccg gcgaactggg ctgtggtcaa cggcgaaact
     9961 gaaacgggcg tgacgaccat gttcatcgag ccgacgctcg acactggtcc aatcctgctg
    10021 caacacaaaa cgccgatcgc ggagaaagaa acggcgccag agttgatgca acggctcgct
    10081 gagattggtg cggagcttct tggtgaaact ctcgcgagtc tcgacacact cacgccgcgc
    10141 ccacagcgtc atgctgatgc tacgttcgca ccagtactga aaaaagaaga cggtttgatc
    10201 gactggtcgc tgagcgcgtt ccagatcgag cgtcgcatca gaggttttca gccgtggccc
    10261 aacgcgtata cgaagttcga ttcaaagagc ctcattcttt gggaagcgca accatcaatc
    10321 tctgtaactt cgggcatttc cgagggcgag gtgatcgtgg cgcaaaagga tgagttggtc
    10381 gtgcagtgtg gccagcgaac cgcgttgcgc gtgctcgagg tccagccgga agcgcgaaag
    10441 agaatggcag cgcgcgattt catcaacggc atgcatttga aagtcggcga tcgctttggt
    10501 taagtcgaaa gagatctcac cggcgcgcgt cgcggctttc aacatccttc agcaggtcga
    10561 gaatggcgcg ttctcttcgg ttctgcttgc agtacaagag ccaaatctac aacctttgga
    10621 ccgagcactg tgtcacgagc tcgtactcgg cgtgctccgc tggcagttgt tcctcgacaa
    10681 gattgtcgag catttctcga gtagacgagt tgagagtctc gatccggcag ttcgcatcgc
    10741 actgcgactc gggctatacc agttacgatt tctcacgcgc attcctgcat ctgcagctgt
    10801 taatgaatcg gtcagtctcg tgcgagcggc gcgactgagc tcggcgaccg cgttcgttaa
    10861 tgccgtgttg cgtcgggcgg tgcgcgaacc tgaatacgac ccagcggctg agatttcaga
    10921 tccgctcgag aagatcgctg tgcagacatc gcatccggca tggttggttg aacgttgggt
    10981 gcgttcgttt ggtatcgaag aggcagaagc gtttgcacgt gcgaacaaca cggtgccgcc
    11041 gaccgcgttt cgagtcgtcg cgaatcgggc aaaccagtcg gagatcttgt cgagattgag
    11101 tgcggcgggc gcggcgctcg agtcgtctga catcgtgagt ggagcatggc gtgtttccgg
    11161 agcgacatcg ttgctgcgtg agatgagcag tgctggtgag atttatctac aggacgaagc
    11221 ctcgcaactc gtcgccgatg cgatggaggt caaacgcggt gaacgcgtac tcgatctgtg
    11281 cgcagcgccg ggcggtaaga cgaccttgat tgccgatcgc gcgcccgacg cggcattcat
    11341 agttgcggct gatcgatccg cgaccagaat ggcgaccgtt atctcaacga tgcgattgca
    11401 cgagttgaag agcatcaaac cactgatcct cgacgcaact gaaccgttgc cgtttgcgcc
    11461 cgggagtttt gaccgtgtgc tcgtcgatgc gccgtgttcc gggacgggaa cattacgcgc
    11521 caacccggaa atcagatgga gactcgcgcc cgcagatttt cagaatttcg cggagcagca
    11581 gaaacggata ctgagccgag cagtcgaggt cttgaagccg ggtggccgtt tgatttactc
    11641 gacgtgttca gtcgagaaag aagaaaatga ggaagtcatt gagagggttg atttgaaggt
    11701 attgaaaact gttcgtacgt ggccgcagcg cgaagggact gatgggttct ttatttcaat
    11761 acttgagaag tagacaggat ttacaagatt ttcatgatta acaggttcat cctgaaaatc
    11821 ttgtaaatcc tgtcccacaa actctgcctt gcccctgatc agggcggatg ctagacttcg
    11881 caacttagtg agtgttgtta ccagaattct gtccgtcttc cgtagcatcg caatcgtgat
    11941 tgcgatcggg atcgtttttc ttttcggagt cgcgaccaca gtctatctct cgctacgtag
    12001 cccggaagtg aaagtccccg acgtggtcgg taagaaccga ttcgacgccg aaaaaatcct
    12061 ggccgacgcc gatctgaagt ttcgcgtacg cgccacgagg cccagcaccg aagcacagcc
    12121 tgacacggtc atgtttcagt tgccgcgtgc cggcgaggta gtgaagaccg ggcagaccgt
    12181 ggccatggac gtcagccgcc tcgccaaagc gggtgagtcg tcggagaacg taacgcccga
    12241 cgagccggca gaaaatcgca ataccaattc agcggcaaac accaacgaga acaagccgaa
    12301 gcggccgaag cccaaaaaca ccaacgagaa cgcaaacgcc aacggcaata caaacggcaa
    12361 cgcaaacaaa tcaatcaatg cgaatcgcgc gaacgtaaat cgcgtcaaca caaatactgg
    12421 cgcaccaaat acgaacgcga ctccgcgcag caacataaac gcgaataccc gaccagcggc
    12481 agtaaacagc aaccgtccgg ctcctcggcc cacaccgaag ccgaaccagt gagtagtgaa
    12541 cagagaccgc cacatcgaca tcgctccatc gattctgtcg gctgactttt cgtgtctcgg
    12601 cgaccagatc aaagccgtcg agcgcggcgg cgcgggcgtg ttgcacgtcg acgtgatgga
    12661 tggtcacttc gtgccgaata tcacgattgg tttacctgtg gttaagtcgc tggccggctt
    12721 tactcgtttg ccgatcgaca cgcatttgat gatcactgat cccggcacct acgcgacgca
    12781 gtttgtgaaa gcgggcgcga gtatggtctc ggtccacgtt gaagcggacg cgcacctgca
    12841 ccggacgctg atgtcgatca aggatgccgg agcgctggcc ggagtggtat tgaatcccgc
    12901 gacgcccgta gttgcggtcg aggaggcatt actctttgcc gattatgttc tggtaatgtc
    12961 tgtcaatcct ggttttggcg gtcagaagtt tatcccgcag gctgtcgata aagtgaggcg
    13021 gctgcggcgc atgattgaag agcgacagtt gaacgtccgg atcgagattg acggcggcat
    13081 cgaccgcaaa aacatcgaaa ctgtcgtggc ggccggagcc gagatcatcg tcgccggctc
    13141 ggcaattttt aatacacccg acgcggaagc ggccgtgaag gagctccgtg acgcgaccgt
    13201 acaatgggtg taggagtggg tttaggcaca aaatgcacaa aaatcatcat gtgtcttttc
    13261 gagctaaagc caaccggacg gaaggaattt gaatccgata gcggtacagt ccggcccgaa
    13321 caggtaactg cccgctttct aaactatttt ttgtgcattt tgtgcctttt tgcggctaac
    13381 tttggaggtt gattttatga ggtctcgctt tcttatcctg gcggttttct gcgccctgat
    13441 ctttggggtg gcacaaaccg gattcgctca gaaatccggc ggccagacag agctctcgac
    13501 cgttcagagg ctggacgtca tgtcctctaa gctcgacttg ttgcgccgct cgctcaacgc
    13561 cgccatcaag gccctcgagc ccaaaaccga caaggacaag aaagccaacg ctgacgatcc
    13621 ggtcgcggtt ttgaagagtc tcgaaaagga agtcggctca gtaaccaacg aagtaaacga
    13681 catccgtaac aagaacgaaa aagccgaaaa attcgacact actgctctcg accgcctcga
    13741 ggctacggtc accgagttga acacgcgcgt cgagacgaac ctgcaacaaa ccgcgggact
    13801 cagaaccacg gccacagcga acaactcgtc cggcgataaa aagaagaaga aagggaagtt
    13861 gtttggactg tttggcggcg gcgacgacaa gtacgctgat ttaaccggcg ccgcggttgc
    13921 cggtcgtgac cgcgtgttgt ttgaagaagc cgcgaaggaa gtgcgcaaag gccatcacga
    13981 taccggtcgc ctgctcttcg ccacgattat caacacctat cccgattctc cgtttctgcc
    14041 actcgcaaaa ctggctatcg ccgactcgtt cttcctcgaa ggcaccacca gcgctctgat
    14101 ccaggcggcg caggcttacc aggattggct gacattcttc ccgacggatc cgcttgctga
    14161 cgacgcgagt ttgaaagttg cggaagcaga gatgaggcag atgggtctct cggatcgcga
    14221 catctcgcac gcccgcaaag ccgagcagcg cttgaaagtc ctactccagc agtatccgaa
    14281 gagccctttg cgagacacgg ttgaagcgcg cctacacgaa gtgcaggaaa atcttgcaat
    14341 gcacaacctg cagatcgcgc ggttctacta cgacgtgaaa tattcgactg gcaaaggcgg
    14401 actaaaaggc gcgcagtcgc ggttgaaaga gatcaccgat aagtatccgt gcttctctta
    14461 caacgacgag gtgttcttcc gtttggcgtc aacttaccag gccgaagaag agccggacga
    14521 agcggcgcgg tattaccaga aactcgtgca ggagtttccg gaaagcgagt acaacgacaa
    14581 agcccgggaa caactgaaca tcatcggagc tccaatccct gaaaagagcg cgccgaatcc
    14641 gtgttcgaag cgtacgcagc agagctttat gggtaacctg atgcaacagg tttcgggcaa
    14701 ggccgacgtc actgtcaaca agaacggaat tctgatcagc aaggacggaa aagaaggtac
    14761 tgatctgatc gacgaggcgc ttcgttacaa cggaacgcta ccggcgttga ctacgcccga
    14821 cgcgcccact cagcgcacag tgaaaccagc agcgacgccg gctgctgatt cgaagtccaa
    14881 gagtgcaaac ggcaacggcg gcgcgcccgc agcagtcgta aagccttaag gaggcttaca
    14941 caaggttgga acccacctcg ccagaagcgc aggcggagtt agcacgacgg cacaagtccg
    15001 cggcgacgac cgtggtgggc ttgctgatcg ccaccgtcct cctctcggtc gtggcttttc
    15061 tgtctgacgc ttacctcgta gagaaaccaa atccacccct cgatatcgcg gtccggatcg
    15121 tcattctgat attcggtttg ggagcggtcg tctggcggcg cacgaagttt cagccgatgc
    15181 ggcttcagga cattgccggg ctggcgggtg tctcgggact gataaagacg ctggaaaaaa
    15241 ccacagttca gatagccttg atcggtgccg ccatcgccgc catcggcttt atcgcgactc
    15301 tactcaccgg aaatgagcgt tacagctact gggcgggcgc gatcgcggtc gtagttttca
    15361 tctactccta tcccaccaaa tcctcctggc tcaagaccat gtaccgcttc accgaaaaat
    15421 aaacacggat tacacggatt gcatacggat tcacaacccg atccgtcttt tatccgtgta
    15481 atccgtggtt aaaaatatgt tgaactatgt ttgtctagtt tgatactctg gacgtggcta
    15541 tgttgcgtct actgccaatt accgggcttt tattggtatt tatcgctatt cctgccttcg
    15601 gtcaggaggc gcgtttgatt acggctctcg ggccaatggc ggtgatatcc acgccggcca
    15661 cgccggtagt cgagcccaac gacatcgaaa aacgagcttt cgagcagacc aatatcgtgc
    15721 gtgtgaagaa cggcctccgc ccgcttacct gggacgcgga tgtctgccgc atggcccgga
    15781 tccactccga gagcatgtcg cgcaaccact acttctcaca cgtcacaccc gaaggaaagc
    15841 gtctccctga gcgcgcccgt gctgccggca ttttgaagtt ttccacgctc ggcgaaaaca
    15901 tcgcctactc taacggctac gacgatccgg gagcctatat cgtcgaacag tggatgcaat
    15961 cttcgaagca ccgcgctaac atactgtcca acgattttcg cgcaatggcc attggtagtt
    16021 ttgtcggccc tgacggttcg atttatctga cgcagacttt catttcacgt tgatcacgac
    16081 gcccgccaag tttccctcct tcgcaaaaat caattggttt ctacgcattt taggtaaacg
    16141 acccgacggc tatcatgaag tcgtgactgt cctgcaaacg atctcacttc gcgacgagtt
    16201 gacgattcaa ctcagtgagg acggaccatt caagctcacg tgcgacgatc caacaattcc
    16261 gactgacaac tcaaatctga tcataaaagc ggcgcatctt ttggaggagt gccgtcagga
    16321 ttggtttgga gccagcattc acttgcggaa gcgaattccg gcacagggcg gacttggcgg
    16381 cgcctcgtct aacgcagcgg tggcgctgct ggcaataaat gagctctgga aaagtagttc
    16441 catcagtgtc gaagaactgc tggatccatc ggatttaggc gccgatgtcc cattcttcct
    16501 ccacggcggg cgctgtgccg caactggcac gggaacggat cttacgccgc tccccgatgg
    16561 tccaaaacag tacctgattg tcatcactcc aaacgctcga gtctcaaccg cacacgctta
    16621 cgcatctcta aatgctgctt ccttgacaac ttttaactcg gactctattc tttcaagttc
    16681 cttagcggac ctgaattccg ccgacagcag gcaatggcct ctgcgcaatg actttgagag
    16741 ggtgatcttt gagatagaac ctgaaatcga gcgcgtcaaa aacgcgctgg ttgatgcagg
    16801 tgcgtggggc gctttgttgg ctggaagcgg ttcgagcgtg tttggtgtct ttgacagcga
    16861 agccgcgaga gatcgcggtc tggagaacct gagatgtgaa gcaggatggc gagtttttcc
    16921 ctgcgaaacc atttcacgcg acgaatactg cagggcaatg aattcatcgg ggtttcccct
    16981 ttttacgctc tcttaaaaac tgcaaacaga aaacagatac tggggcgtag ccaagtggta
    17041 aggcaccggt ctttggaacc ggcattcgtt ggttcgaatc cacccgcccc agccaaattc
    17101 ttgtcactct gaaaagatat gagcactacc ggcggcatca agatcttttc tggaaatgcg
    17161 aaccccaagt tggcggaaga gatctgtcgt tcgctgactt gcgacatggg gaaagcgtac
    17221 ccggagcgtt tctctgacgg cgagttcaat tttcagatcg atgaaaacgt gcgcggcggc
    17281 gacgtcttca tcgtgcaacc gacttgccct ccgaccgatg cgcacctgat ggagctactg
    17341 gtgatgctcg acgcttttcg tcgttcatca gccgagcgca tcacggccgt gattccttat
    17401 tacggctacg gcaggtctga taaaaaggac cggccgcgcg ttccgatctc ggcgaagctg
    17461 gtggcgaatt tgatcaccgt cgcaggcgct gataggttat taacgatcga cctgcacgcc
    17521 gcgcagatcc agggcttctt tgatattccg acggaccacc tgtatgctgc accggttttt
    17581 atcggctatt atcaggagca cccgctgccg aatttgacgg tagtggctcc cgacaccggc
    17641 ggcgccgaac gcgcgcgcgc ctacgccaaa cggcttaatg ccgatcttgc tctttgcgac
    17701 aaacgtcgcg agaaggcgaa tgtggccgag gttatgaacg tggttggcga tgttcgtggc
    17761 cgcagttgtt tgatcgtcga cgatatgtgc gacaccggcg ggtcgttgac gaaagttgcg
    17821 cgagcgttga aagaagctgg cgccgaacga atacacgcgt gtttcacgca tccggtgttt
    17881 tccggcaagg cagtcgagtt gctgcgcgag tcggacattg aaaaagttat cgtaactaat
    17941 acgattccgc tccgcgatca tgcgtgcgag ctggataaaa taaaagtact aagtatcgcg
    18001 ccgcttctgg cgaaagcgat caagtcgatc cacgaggaga cttcagtttc ctcgctcttc
    18061 gtttagacga agggcaaaca cctgatggct taatgaagac catcagcgac ctgggccacc
    18121 aagtaatggc ggcccgggtc gctgaaagtt tgtgtaggga gaaaatcatg gcggagaaaa
    18181 aagaatacaa ggtgcaggca aaggtgcgcg atggtcgcgg caagaatgac gcgcggcgtg
    18241 cgcggcgcga ggggctagtg cccgttacgg tttatggcgg tggtgcggaa accgtcgccg
    18301 ccgtcgctcc gctccgtgaa ctggctgcaa tccttcgttc tgattccgga cgaaatacga
    18361 tcttcacgat cgacgtcgag ggtgtcggcg cgagtgaagt gatgtttcac gatcggcaga
    18421 tcgatccggt caagggtcgt ctgattcatg ccgatctgac gagactcgtt aagggacaga
    18481 agatcgaagt gacggtgccg ctacatctga ctggtgagcc cttcggtgtt cgtgagaagc
    18541 agggcgtgct ggaacagatc attcgcgagg tcgatattcg ttgcgagccc cgcgagattc
    18601 ccgacacgct cgaagttgat gtgaccaatc tcgacgttca cgagacgttg cacgtttcgg
    18661 atatcaaggt tgctgaagga gtcgagatcc tgaccgatgg cgagcaggta attgcgactg
    18721 tcggcatcgt gaaggaagaa gtcgcagccg cgcctgctga aggtgaagag cctgccgagc
    18781 ccgaagtgat tggtaagggt aagaaggaag acgaagaggg cgacgaaggc tagagaagtt
    18841 gttagacagg attacgggat ttccaagatt tacaagatct atcctgttaa tcctgaaaat
    18901 cgtgtaaatc ttgtgaagct tatcgtcggt ttaggaaatc ccgggaaaga gtacgagtcg
    18961 acgcgacaca atctcgggtt catgttgatt gacaagttgg cccacgacga gggcatcagt
    19021 gttaacagcc gcgagtgcag ctcgctcgtt ggtcgcggcg tgattgaagg cacgcggacg
    19081 aagttagtga agccgcaaac cttcatgaac ctgagtggac acgcggtcgc ctgtctgctc
    19141 aagaagatcg aaagcgagtc gcctatcgat catctggtag tgatttctga cgatctcgcg
    19201 ttgccggttg ggaaaattcg catacgcgag cgcggctcgg ctggtgggca taacggtttg
    19261 aagtcgatca tcgcgtcgat cggaacggat gagttcatcc gcttgcggat cgggattcag
    19321 ccggaacatc cgatctcgga ttcgaagcga tttgtgctcg acacgatttc acgcagtgag
    19381 cgagcaatct tcgaagagac gatcgcgcag agtgcagacg cgatacgaac gattatcagg
    19441 gatggcgctt tgaaagcgat gagtattttt aattgaattg ggtgaccttt gactggaggt
    19501 ttgagttgga tacaaagaga cagtacgaag tggtcttcat agtagacccg acggccgatg
    19561 atgaagtgcc gaaactcacc gagagcttct cgcagatcct taccgatcag ggcgcggtgg
    19621 tgatcaaggc cgagacgatg ggcaagcgcc agctcgcgta cgaaatccag caccataaag
    19681 acggcacgtt tgtgttgatc gagatggagg gctcgggcaa ggagattgcc gagcttgaac
    19741 gccgcatgcg cgttaatgat cgcgtgctgc gctacatcac ggtgcgcgtg gatgaagatc
    19801 gcaggcgtgc ggagaagttg aaggctcgtc gcgctcgtaa agctgcgaag cgccccgcgc
    19861 ctggtgagca ccggcgtcgt ggacccgcga tggagtttgc cgaagcttcc ggcgaataaa
    19921 ggatcgagac tttaagagga taagttatgg caaccaatta tgaagaccgc cagcgcggcg
    19981 gtggcggtgg ttacgatagt gacgatgatg gcgatagacg gccaatgggc ggcggccgcg
    20041 gcggtggtcg tcgtatgcac cgccggcgcg tttgccggtt ctgtatcgac aaagtagacc
    20101 ttatcgattt caaggacgtg aagttgcttc agaactactt accggagaga ggcaagatcc
    20161 tgccgcgacg catctccggc aactgtgcaa cgcaccagcg catgctggcg gaagcgatca
    20221 agcgcgcgcg aaacatcgcg ctgcttccgt acacgaccga ctaaagggag aacgagatgg
    20281 cacacacgaa agtactcttg cgcgaagatg ttgacgatct cggcgcgcgt ggtgagattg
    20341 tcagagttag atcgggctac gctcgcaact accttctgcc acgcaatctc gcagttgaag
    20401 cgaccgcggg caacgtcaag ggaatcgaat ccgagcgtgc ggcgttgttg aagaaggaag
    20461 cgaaggagcg ctcgagtgcg gaagcacagt cgcaacagat gggcgcgctc gagctcgagt
    20521 tcaagcgaaa agccggcgag cagggactcc tttacggatc agtcacttcg atggacgtgg
    20581 ctgaggcgtt gagagagcgt ggctacgaga tcgatcgaca tcgcattcac ttgcgcgagc
    20641 ctttgaagcg tgttggtgaa tacaccgtac cggtccgcct gcatcgtgaa gtcacgatcg
    20701 atctgaaggt cagagtcgct ccggaaggcg aagtgatcgt tgggcatttg tcgccggaac
    20761 aggaagctga attggcgaaa accaacgagg aagaatcaaa cgcggattaa cgcggatctt
    20821 taaaacacga tttagggatt tactactgaa aatccattgc tcattattga cgctgcatgt
    20881 taatacgacg tgcagcgttt tcttatggcc acccctgacc ctattcgcga acagcccttc
    20941 gaacgggctc tcccaaacag tgccgaagca gaacgagcca tactgggcgc cgtcctgctc
    21001 gataacggcc tgatcagcca ggcaatcgaa caactccgtc cggaagattt ctacgtgcct
    21061 tcgcatcgtc gcgtcttcgt cgcgatgatc gcgctcttcg agcgtggcgc cgagatcaat
    21121 ccgattctca taggcgaaga gctgaagaaa gagagctcgc tcgaagcagt cggcgggatc
    21181 agtttcatta ctaacctggc ttacggcctg ccacactcaa ccaacatcgc tcactatgcc
    21241 aaagtggtga gaggaaaatc gatgttgcgg cagctcatca aagcttccaa caagatcaca
    21301 caggaagcac tcgctgaaga agacgagccc gagctcattc tcgatcacgc cgagcatgca
    21361 atttttcaac tcgccgacga acgagtaaga caaggtttca tgcacgtgaa accaatcgcg
    21421 gagcaactgc tcgagaaagt gcaggagatg gaaggccgag cggctgtcct gaccgggctc
    21481 accaccggct tcgccgatct cgacaagatg acttcaggac tgcagaactc cgacctgatc
    21541 atcctcgccg cacgaccgtc gatgggaaag accagcttcg cgttgatgct ggcggagaat
    21601 gcggcgattc tttccggcgc gtgcgtcggc gtcttttcac tcgaaatgtc gaaagagtca
    21661 ctcgtgatgc gcatgctttg tgcgcagggc aacatcgatg cgcagcgttt ccgcaatggc
    21721 tttctctcgc gactcgagtg ggcccagatc gcgaagtcac tcggcgtgct ggctgacacg
    21781 aagatctttc tcgacgacac accgggactg agcgtcctcg agatgcgcgc gaaagcgcga
    21841 cgactcgcgg cggaacagaa aaaactcgat ctgatcattg ttgactactt gcagttgatg
    21901 agtggctcgt cgaagcgtac tgaatcgcgg cagcaggaag tgtcgcaaat ctcgcgcgag
    21961 ctgaaagggc tcgcgaagga gatgaacgtg ccgctcgtgg ccttgtcgca gctttcacgc
    22021 gctcctgaat cgcggtcgga tcaccgtccc caactggcag acctgcgtga atcgggcgcc
    22081 ctggaacagg atgcggacgt agtggccttc atcttccgcg aagagcagta caaaacagcg
    22141 gaagaacgcg aggccatgcc cgaagatcga aagaacatcg ccgagctaat cattgccaaa
    22201 caacgcaatg gtccaaccgg aaccgtcgac ctccggttca ccccctcgag catgaggttt
    22261 gataaccttt atcgagagta gggagactga tctccgaacc ttcctcacga gcacttgcca
    22321 cggtcgttga gatcagatca caatctttct ctccctacca aatcctatga aaactagaaa
    22381 atcgaccttg caactcgtct tcatactggc gctgctcgta tgcagcggtt cggctgcgtt
    22441 tgctcaggct ctcagcatca agctgagcgt agatgcgacg caggctgcgc gcaacatact
    22501 gcacaccaaa ttcacgattc cagtgcgacc gggaccgctg actctcttct acccgaagtg
    22561 gattccgggt gaacacagtc cgaccggtcc gatcaacgat ctcgttggct tgcgtttaag
    22621 cgcgggaggt aaaccgatcg cgtggcaacg cgatcaggtg gagatgtttg cgtttcattg
    22681 tgaagttccg cagggagtaa atgagcttga ggtttccttc gacgacgttt ctcaaccaga
    22741 aacaaccgcg agtgcaaaac tggcgcgcat caagtggaac agactgattc tttatccgag
    22801 agatttgaac agcgacacca ttcaagtacg tgcctcattg cagttgccgg ctggatggaa
    22861 gtttgcgagt gcgttgccga tgctgagcga gaacaaagat ggatttcaat tcaatgaagt
    22921 atcgctcacc gagctggtcg attctccggc gatcatcggc gcgaactttc gcaagtttgc
    22981 actaacgtcg acaggcatca cgcatgagat cgacgccgtg gctgatactc ctgcggcgct
    23041 cgtgatgaag ccggagacct taacgggtct gaaaaacctg gtgcaggaag cttacgcgtt
    23101 gtttggggcg cgccactacc ggagctacaa atttctggtt acgttcagcg accatggcgg
    23161 cagcgagggc ctcgaacatc acgaatcgag tgaggatggg gttggcgaga aggcattggt
    23221 tgatgaactg gagttgattg atttcgccga cttgatgggc catgaatatg cccactcgtg
    23281 gaacggcaag taccgccgtc cggcagggct caccacgcct gatttcgagc aaccgatgaa
    23341 gggcgagctg ctgtgggttt atgaaggatt gactgaatat ctcggccgcg ttttgccggc
    23401 ccgcagcgga ctgtggacgg cggatgactt ccatgaagcg atggctgccg tggctgctga
    23461 gttcgaaatg cagtcaggcc ggcaatggcg gccgctcgta gatacagcgg tggcagtgca
    23521 gtttacttac ccctcgccgc gcgcgtggat gaattaccgt cgccgcgttg actattacga
    23581 tgagggctcc ctcgtctggc tcgatgcaga tgtgctgatc aggcaacgca gtaacggcaa
    23641 actgtcgctc gatgatttct gtcgccgctt tcatggcgga tctgacacgt ctccgtcagt
    23701 caaaacttat accttcgatg acgtcgtgaa tacacttaac gaagttatgc cgtacgactg
    23761 gcgcggcttc ctgaccgaaa gaatctatca ggttgcgccg cacgctccac tggcaggcat
    23821 caccggcggc ggctggaaac ttgtctacac agataaaccg aacacactgg ttcgtttagg
    23881 agatcacgct cgcaagagtg tcgatcttat gtattcgatt ggggccatgt tgaaagaaga
    23941 cggcagcgtc atggatgtga atccaactct cgaagcagcc agagcaggaa tcgcgccggg
    24001 tatgaaaata atcacggtga atggccgagc atggtccacc gatgcgcttc acgaagcgat
    24061 tacagcggcg aagagcagta ctacacccat cgagttagtg gtcgagaacg gcagttttac
    24121 cggcgtgtac aaattgaact atcacggcgg cgaacgttac ccacatctcg agcgcgaccc
    24181 gacaaaaccg gacatgctgg gagaggtgat caagtcgcgg cgttaaggtt tcccaaagat
    24241 tttggcgtca atagggacgt tggtcttaat ttcgttcact gtgagcgtca tcgtccgctt
    24301 ctgaactgtg aaggtgatca catgcggcag tttaagtccg tcagcttcac gccaatcatc
    24361 aaagtaaatt tcagcgcgtg gggtgttcat acggaccggt aagtagcttt cagcatcaat
    24421 aaagagccga tccgaggcgc cactcaccgg cttcatatcg atcacgtaaa cctcccgata
    24481 gccgatcttg cttttgccga ccagggtgaa cttcgcatac ttatttttga ggttgataat
    24541 cgaatagaag tcggcatcgc cgctcatcac aaccggctca gctccagcag gttttatgat
    24601 gccgacagac ttattaggcc ggaccgtgta agtttcgaac gtcccacctc tgctcacacc
    24661 gactatgtcg accgtgcctt taaccacacg actggtcacc gcattcacgg ccgcagcgcc
    24721 cccaatggct tgcacatact tgtcgagtac ctcatcaatc gccggcaacg cgcctgagtt
    24781 gtcgaccgtt tgtccatcgt tgatagctac aggaccacca tcaccgccca gcatgcgcga
    24841 ggatgtcgtc cgggttggct cggcgatcgt gtcgcccttg atcgcttcgc gacgcccgcg
    24901 cgtgttcgtg ctgcccgcac ggtactgttt gatgtcctgg accagctcgg cgtagttcgc
    24961 attagctggt gacagttgct gcgccttttg ggccatctcg agcgcttcgt cagcctggtc
    25021 gctgaggaag tcgacgacgc cgagtaagta atacgccggt gcataattcg gagcgagctt
    25081 gactgattgc aggagctgtt cgcgcatcat cgcagcattt tcgcgcgagt attgcgagac
    25141 ctcgccgttt gcagagattc cttcacgact caaaacgtac gcgtaatagt agtggaccag
    25201 atagctctgc gttgaggagg tcgccttctg gagataattc ttcgcctccg cgtagcggcg
    25261 ttgctcgaca tacatggaac cgagcgccgc ataggcaggg agaaaccccg gatccatcgc
    25321 aatcgcttcc ttgagaccgc gctcggctac cgtgtagcgg cccatgtgta gcgatagatc
    25381 gctgaggtaa tagtacgctt cgctgtcagt cagcgctgac cgttgcgtgg ctgtgtaaga
    25441 acggtaagct tccggattag ccacacccgt aatacgttgc gtcgtcaggt tcccgcgccg
    25501 cacgtaaaat tgcagctctt gctcgatcac atcgagagtc gttccgtaag ttccttcgat
    25561 cgccttggcc gcatcatcgc cactggcgat acgttgcaga aatcgcttga actgatccgg
    25621 tcgcgcgcga tcgccgaaca tcaggtaatg cacgagggcc cacgactctc catagaagat
    25681 cccggcttta tcacgttcgt tgtagtgtgg cgagtcaagg ccgatcgaaa agagtgtttt
    25741 caacggcagc aattctgcct ggcgcagagt gtaaaggtaa ttgtggtccg gcatgccgat
    25801 gatggcgtcg ccaccggaga attgcagcgc ctcgtagagc tcggcgagtc cttcgttaag
    25861 ccaaactggc gcgttgggca cgttatcttt gacgtgcagg tgaacgtatt cgtgaaacgc
    25921 agtaccgtac ggatctccgc tcggatcgag cgagaatgcg atgtagttga cgtcatcgcc
    25981 gggcaggaaa aaaccggaaa tactggtcgg gcgtccgttg taaaccggct tgaacggcgt
    26041 aaacgaggcg tcgtcacgaa agacgataac tgttgttggg atggacgagt cgatcacatt
    26101 gcgcgagaca agacgagcga aggcggcatg gaaatactcg agccagaccg cgactcggcg
    26161 caggttttcc ggatcggcat tgccgataac gaagagatga ttggtgcgga cgctattcca
    26221 ccgggcgcct gtttgcgcgg tgatagtcga gaacgtaaag agaaaggcga taacgagggt
    26281 aatgggagtt tttaatttca taacgaggtt tgtgaggtag gagacagtct aagacaggat
    26341 tacgggatta acaagatttc aaatctcgta atcccgcaat cctgtctaca aacagtgtgt
    26401 gtgaaggaca aactcggaaa actactaaag aacctcttcc aatagcgcag ccaatctcca
    26461 gccacccttt tgaatctgtg ttctcaccac atcagccgcc cagactgcat aaaactggcc
    26521 gccgggtccg tgcttcttct caaacgcttt gccacgcctg acgagggttt gagcctgatc
    26581 cagttcgatg ttgtcgaacc tgagtcgctg gtgtgcctcg cgagcagtcg ggagaatgcc
    26641 gtttgctatt tgctctgccc aggtacttac gtcgccgttc aactgccaat tcgcaggcgc
    26701 ttttgagccc agtt
//
LOCUS       DASSFQ010000007        11887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_751181, whole genome shotgun sequence.
ACCESSION   DASSFQ010000007 DASSFQ010000000
VERSION     DASSFQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751181"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..575
                     /locus_tag="VFH96_00240"
     CDS             99..575
                     /locus_tag="VFH96_00240"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET6972413.1"
                     /translation="MSYTSFMPSVALVEPEIPPNTGNIARLCAATNVPLHIVGVTGFR
                     LDDRAVRRAGLDYWPEVQLTRHKDLAALYESLPQARFVYLTTKAEKTYTDWSFRPSDC
                     LVFGRETRGLPEDLLRANWSNCLKIPMQNPRIRSLNLATAVGIVLFEALRQLSRSA"
     gene            595..2415
                     /gene="typA"
                     /locus_tag="VFH96_00245"
     CDS             595..2415
                     /gene="typA"
                     /locus_tag="VFH96_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET6972414.1"
                     /translation="MQLRNDIRNIAIIAHVDHGKTTLVDAMLRQSGTFRENEQVRERV
                     MDSMDLERERGITIMAKNTAVRYHNFKINIVDTPGHADFGGEVERVLKMVDGVMLLVD
                     AAEGCLPQTRFVLRKALEARLPAIAVVNKIDRSDARPAEVVDEIYELFLDLDATDEQI
                     EFPILYAVSRDGTAKRKLEDQSANLQPLFEQIVESIPAPRELRTDALQLLVANLDYND
                     YVGRLAIGRIFSGEIKIGDQIAVVKPDRSIHKTRVSQLYAFEGLKREPVERAGFGEIV
                     ALAGIENINIGDTITSIENPQPLPSIAVDEPTIAMIFGVNNSPFAGRDGKYVTSRQVK
                     ERLDKEILGNVAIRVEETDSPDQFKVSGRGELQLAILIEMMRREGYELQVSKPEAITR
                     KVEGKTLEPIEAVVIDCPDEFIGVITEALGRRKGQMTKMVNHGTGRVRLEFEAPSRGL
                     IGFRNEFLTETKGTGLLNTMFLRWGEWQGALRGRSTGSLVADRLGDTTTYALFNLQER
                     GLLFVRPGTKVYEGMIVGENARAVDLDVNAVKEKKLTNMRASTADEAMRLVPPKELSL
                     EQALEFIADDELVEVTPQSIRLRKRVLASNMRPKKKEAAE"
     gene            2412..3068
                     /gene="thiE"
                     /locus_tag="VFH96_00250"
     CDS             2412..3068
                     /gene="thiE"
                     /locus_tag="VFH96_00250"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET6972415.1"
                     /translation="MIRVDPSFQCNPWLRLPRVYALTDVGLSGLSHAEQVELLSAGGA
                     TLIQLREKGMPAREFYAQAQEAVEVAVRRGVQLIVNDRVDVALAVGAHGVHLGQDDMP
                     PEAARRLLGPEKVIGYSTHNIDQAIAATKLPIDYLAIGPIFATTTKTDTAPVLGLEGL
                     RTVRQAIGPIPLVAIGGITSANAADVIAAGADSVAVISTLLSNSSQISEATHSLLQDL
                     "
     gene            3167..4381
                     /locus_tag="VFH96_00255"
     CDS             3167..4381
                     /locus_tag="VFH96_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET6972416.1"
                     /translation="MHSKGRLLSIPYLMSFFELPPIIERMLLVSDKISDLNFSVGQLP
                     QVEINGKLTPVQPLGMQKLSPYQTEIIALTLMNGNPDAAERLARTGTADLSYSLPSRA
                     RFRVNIFQQRGVYSIVMRVIPTDIPTLKSLGLPPHLTDVSELRNGLVLVTGPTGSGKS
                     STQAAIIDIINEKKYYHIVTIEDPIEYLHLHKRSTINQREVGHDTRDFPSALRATLRQ
                     APKVILIGEMRDFETTEIALEAAETGHLVLSTLHTIDASKTVDRIIGLYPKNEEPVIR
                     TRLAQTFRYIVSQRLIPRADGNGRIAAVEILRSSPRTREYIEVGEGDGKSLLDAMRDG
                     KLDGMQDFDSVIKGLIESKVVSLEDGLAFATNQNNLLLQLKGMTASEDFIRRDGVPPT
                     FSDSGSMLGMIE"
     gene            4385..5155
                     /locus_tag="VFH96_00260"
     CDS             4385..5155
                     /locus_tag="VFH96_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET6972417.1"
                     /translation="MIIVCPKCSTRLQVDDEKSPNRPFNVRCPKCSATVSSGPSSPAT
                     EQSALAVGGSPSTDHPRFEPTTARAYEPSTPGAPGVTSATPANDDTVRMLFDMLSKST
                     NPSSENPNARPSWDKRKALVCTSEPYRELVASKLSEVGCQVFVAEETRQAVETMRANK
                     MDVVVLEPQFDPAEQGSVFVMREINVLRPPQRRRLFFVLLSPSLRTMDAHAAFLNNVN
                     AIVNVTDIEELSRIMEVGLREYNELYREFYTSFGLTAL"
     gene            5268..6215
                     /locus_tag="VFH96_00265"
     CDS             5268..6215
                     /locus_tag="VFH96_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6972418.1"
                     /translation="MGQQEHTHGHQHKNGSWRRLSLVLLLTMLYMFAEVIGAWWTGSL
                     ALLADAGHMFTDVAALILALTAVWFGSRPATSRKTFGYYRLEIIAALVNGVALVVISI
                     FIFYGAYERWQSPPVVKTGPMILVAIGGLLVNLACAWILHSRQEVDLNMRGAWMHVMG
                     DALGSVAAILAGVCMALFGWYAADALFSVVISLLIIWGSVRLIRESTNVLLEGTPAHI
                     NLAAVENAILDTTGVSDVHDLHVWTITSGRDALSAHVIHTPSISQPDLLRELRTKLHD
                     RFGVDHLTIQMETPDFEDETFHFCHAGTACFRSDQNRAR"
     gene            6215..6457
                     /locus_tag="VFH96_00270"
     CDS             6215..6457
                     /locus_tag="VFH96_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972419.1"
                     /translation="MELNLLLTLDLREQAALQAALMTHGAPDSLVTLALTGACRMPSL
                     EEARQLRKWLAETRTAGATDFAAFHVIEKALIDFGL"
     gene            6483..7757
                     /gene="larA"
                     /locus_tag="VFH96_00275"
     CDS             6483..7757
                     /gene="larA"
                     /locus_tag="VFH96_00275"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HET6972420.1"
                     /translation="MAQLELSYGRRSLEFSFDEARFSVLTTDRIPKTPLKDFEIGLAL
                     DDPIASPPIDELVSSDDSVLIVVSDATRATASAQIVNLLSRRLVQAGVSPASMAVIFA
                     TGIHRPVTEKEKVELLTPFIVQRLRILTHDAYDQSKLHAFGKTESGVTVELNSALNEF
                     SRVFLTGGITFHYFAGFTGGRKSICPGLASAKTIEATHMLALDFASGGRKTGVRAGAL
                     DGNAVHEECERVASLVAPTFSINTIVNEDKQAVRLYAGDWRLAHRAACDYYLEHFSVP
                     IPSKRDIVIVSTGGFPHDINLIQAHKALDMAALACNDGGTIILLAECSDGLGRPDFLK
                     WFSKADARALAARLVNGYEVNGQTAWALLTKAERFRVCLVSELPPDDVRRMRMIPVRT
                     IEEALEQAGNGEGFVMPRGAAVLPLHRSVETT"
     gene            7754..8269
                     /locus_tag="VFH96_00280"
     CDS             7754..8269
                     /locus_tag="VFH96_00280"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET6972421.1"
                     /translation="MNLRLASCTVRNWEWRDRDAIVRYGNNRKVSINLRDRFPFPYTA
                     RDARNWLDMVVDAKPVTNFAIDVAGEAVGGIGFTPQHDVDRRSAEIGYWLGEPFWGRG
                     IATEALMAVTEYAFANFDLCRLYAHVFAWNAASARVLEKAGYEFEGRLRKSVTKDGQT
                     IDQLMFAKIRE"
     gene            8356..8925
                     /locus_tag="VFH96_00285"
     CDS             8356..8925
                     /locus_tag="VFH96_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972422.1"
                     /translation="MKMMAVGLMIVCSALLASAQELAGGLNEQRVALTQAAVALDASG
                     APALEATLRSTALNGAPETPVTNVRIVVKNRSPFPYAFVSGAVTFYDAAGVRCGEGVF
                     KADAIAVDESFESDLPGLRIRCEAATWRIIATSLLPRIPPNAPIPALARTPSNFIISI
                     DGETHPIQLDKPLTVTLGEKRRTIIVRAP"
     gene            8928..9509
                     /locus_tag="VFH96_00290"
     CDS             8928..9509
                     /locus_tag="VFH96_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HET6972423.1"
                     /translation="MLSTLIELQRLKRLDRTGWTLRGLPNGTESVAAHSFGVCVTAML
                     LTDCCKARGVAIDVEKVLRIALLHDWAETRVGDMPRTATLYFGSAARKQAETAAFADI
                     VDPLDADSSYANLYDDYERRESTEARLVKAADVLDLLIQVLALERAGARGLDEFWEVA
                     ENPEFKLEGVAEQIVLELLGSILKARSELQPNV"
     gene            9502..9930
                     /locus_tag="VFH96_00295"
     CDS             9502..9930
                     /locus_tag="VFH96_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972424.1"
                     /translation="MFDFLSDFFFSITWRKALIGAAIFLVSFFANLGVVTLILVKLRA
                     DHFSKSRKTKFWSGPRPWLHAAKVIGKNIAGVLLVALGIVLSLPGVPGQGLLTVLLGV
                     MLLDFPGRHRLEQKLLSRPSIVNAINKLRERFEKPPLQLN"
     gene            9978..11030
                     /locus_tag="VFH96_00300"
     CDS             9978..11030
                     /locus_tag="VFH96_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972425.1"
                     /translation="MLKYFNAHRIVPSRLLLSVLAVSILLFTAGCGLFGAKKKITVPQ
                     LLGPLAKADKARLIQEVNRLSTVKSIHGKVDIQFEDTSFASSGIADQYRLVDGTITLQ
                     RPGQIYLIIQFTFVDIAQMASDGEHFRVAVLKGDDKYKRFVKGTNSADYGKFDADGDA
                     QAQQTNQQKTEKVTINSLSNLRPQHLTDAFMIRPIDSNSSLVYAQSEFFNEEPDVRRQ
                     AKKDARVMRGYYFLDEFSTAAPGDAKLVRRFWFDRVSGIRLARVQSYDERGQLITDVS
                     YSNEKALGSGPTASLPSRVEITRPQDQYKLSISYQDSASVELNREYPPRAFVLENKWG
                     LPEVDLDSQNKKTANK"
     gene            11045..11737
                     /locus_tag="VFH96_00305"
     CDS             11045..11737
                     /locus_tag="VFH96_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972426.1"
                     /translation="MRTILQAEDLVKVYRIGKVDVPALRGVSLEVEEGEFVAIMGPSG
                     CGKSTMLHLLGGLLTPTSGRIVIDGEDITAASDAKRTAIRRKKIGFVFQRFNLFPTLT
                     AEGNLRLAERIHGNGGGNSQLRREVLRLLGLEDKLHHKPLELSGGEQQRVALARAVVT
                     RPAIVLADEPTGNLDSENSALVLNMFQELNYRFNQTIIMITHNPEAAAMCKRIVQMRD
                     GHIVEQTPTVRL"
BASE COUNT         2571 a         3271 c         3433 g         2612 t
ORIGIN      
        1 acagtactta ccttctaccg gcgcacacaa acatctgagg ccgacgaggc cttacaaaag
       61 aaacgacgag tccttcatgc gaagctcgtc gtttcttttt gtcctatact tccttcatgc
      121 cgtcagtcgc tcttgtagaa cctgagatcc ctcccaacac cggaaacatc gcgcgtctct
      181 gcgccgccac taacgtgccg ctgcacattg ttggtgtgac cggcttccgt ctagacgatc
      241 gcgcggtgcg acgagccggt ctcgactact ggcctgaagt gcagctcacg cgtcacaaag
      301 atctagctgc gctctacgaa tcgttgccgc aagctcgctt cgtttatctc acgacgaagg
      361 cagagaagac ttacactgac tggagttttc gtcccagtga ctgtctcgtc tttggccgcg
      421 agacgcgcgg cctgccggaa gatctgctgc gtgctaattg gagcaactgc ctgaagattc
      481 cgatgcaaaa tccgcgtatt cgcagcctga acctcgccac cgcagtcggc attgttctct
      541 tcgaagccct ccggcaatta tcacgatccg cctgattcgt ggctaaatag ctccatgcaa
      601 cttagaaacg acatcagaaa catcgccatc attgcgcacg tcgaccacgg taaaaccact
      661 ctcgtcgacg cgatgctgcg acaatcgggc accttccgcg aaaacgaaca ggtgcgcgag
      721 cgcgtgatgg attccatgga tctcgagcgt gagcgcggca tcacgatcat ggcgaagaac
      781 acggccgtcc gttatcacaa cttcaagatc aacatcgtcg acacgcctgg ccacgctgac
      841 ttcggtggcg aagtcgaacg tgtgttgaag atggtcgacg gcgtgatgct cctcgtcgat
      901 gcggccgaag gctgtctgcc gcaaacgcga ttcgtcttgc gaaaggcgct cgaggcgcga
      961 ctgcctgcga tcgccgtcgt caacaagatc gatcgcagcg acgcgcggcc tgcggaagtc
     1021 gtcgatgaga tctacgagct gtttctggat ctcgacgcga ctgacgagca gatcgagttt
     1081 ccgattctct acgccgtctc gcgcgacggc accgccaaac gaaagcttga agatcagtcc
     1141 gcgaatctgc agccgctctt cgaacagatc gttgaatcga ttccggcgcc gcgcgagttg
     1201 cgcacagacg cgctgcaact actcgtcgcg aatctcgact acaacgacta cgttggtcgt
     1261 cttgcgatcg ggcgcatctt ctccggcgag atcaagatcg gcgaccagat cgcggtcgtc
     1321 aaacccgatc gttcaataca caagacacgc gtgtcgcagc tctacgcttt cgaaggattg
     1381 aagcgtgagc cggtcgagcg cgccggcttc ggcgagattg tcgcgctcgc cggcattgag
     1441 aacatcaaca tcggcgacac catcacctcc atcgagaacc cgcagccgct gccgtcgatc
     1501 gctgtcgacg agccgactat cgcgatgatc ttcggcgtca acaactcgcc gttcgcagga
     1561 cgcgacggga aatacgtcac gtcgcgacag gtcaaggaac gactcgacaa agagatcctc
     1621 ggcaacgtcg cgatcagagt tgaagaaact gactcgccgg atcagttcaa agtgtccggc
     1681 cgcggcgagt tgcagctcgc gatcctgatc gagatgatgc gccgcgaggg gtacgagctt
     1741 caggtgtcga aaccggaagc gatcacgagg aaggtcgagg gcaaaacgct cgagccgatc
     1801 gaagcggtgg tgatcgactg tcctgacgag tttatcggtg tgatcacgga ggcactaggg
     1861 cgacgcaagg gacaaatgac gaagatggtg aatcacggga ctggccgcgt tcgtctcgag
     1921 tttgaggcgc cgtcgcgcgg gttgatcggg tttcggaatg agtttctgac ggagacgaag
     1981 ggaaccggtc tgctgaacac gatgtttctg cgttggggcg agtggcaggg agcgctacgc
     2041 ggacgatcga cgggttcgct cgtcgccgat cgactgggcg acacgacgac gtacgcgctt
     2101 ttcaacctgc aggagcgtgg gctgttgttt gtgagacccg gtacgaaagt ttacgaaggg
     2161 atgatcgttg gtgagaacgc gcgcgctgtc gacctcgacg tgaatgcagt gaaggagaag
     2221 aagttgacga acatgcgcgc gtcgacggct gatgaagcga tgcgactcgt gccgccgaag
     2281 gagttgtcgc tggaacaggc gctggagttt attgcggatg acgaactcgt agaggtgacg
     2341 ccgcaaagca ttcgtctgcg gaagcgcgtg ctcgcgtcga acatgcgacc gaagaagaag
     2401 gaagccgctg aatgatccgt gttgatccgt cttttcagtg taatccgtgg ctaagacttc
     2461 cgcgggttta tgcgctgacg gatgttgggt tgtcggggct ttcgcacgcc gagcaggttg
     2521 agttgttgag tgccggcggc gcgacgctga ttcaactccg cgagaagggt atgcctgcgc
     2581 gtgagttcta tgcacaggcg caggaggctg ttgaggttgc ggtgcggcgc ggagtgcagt
     2641 tgatcgtcaa cgatcgtgtt gacgtggcgc tggccgttgg tgcgcacggc gttcacctcg
     2701 gccaggacga catgccaccc gaggcggcgc ggaggttatt aggcccggag aaggtgatcg
     2761 gctactccac gcacaacatc gaccaggcca tcgcggccac gaaactcccg atcgactacc
     2821 tcgcgatcgg cccgatcttc gctacaacta ccaaaaccga cactgctccg gtactcggac
     2881 tcgaaggcct gcggaccgtt cggcaagcga ttggcccgat tccgcttgtg gcgataggcg
     2941 gtatcacgtc tgcgaacgcc gccgacgtca tcgctgcggg tgccgactct gtcgcagtga
     3001 tcagcacgtt gctatcaaat tccagccaga tctccgaagc cacccacagt cttctacagg
     3061 atttataaga ttagacagga tttacatgtc aatcccgaga aatcatgtaa atcctgtcta
     3121 aacataattt tcttgcgttt gtcattgtta tgcgcgaaaa tcgcccgtgc attcgaaagg
     3181 gcgcctcctt tcgatcccct acctgatgag tttcttcgag ttaccgccca ttatcgagcg
     3241 aatgttgctg gtgtcagaca aaatcagcga cctgaatttc tctgtcggcc agctcccaca
     3301 agtcgaaatc aacggcaagc tcactccagt ccagcctctc ggaatgcaga agctctctcc
     3361 ctatcagacc gagataatcg ccctcacact catgaacggc aacccggacg ccgccgaacg
     3421 cctcgcgcgc accggcaccg ccgacctgag ctactcgctc ccctcgcgcg cgcgttttcg
     3481 cgtcaacatc ttccagcaac gcggcgtcta ctcaatcgtc atgcgcgtca tcccgaccga
     3541 cattccgacg ttgaaatccc tcggtctgcc gccgcacctg accgacgtct ccgagctgcg
     3601 caacggcctc gtcctcgtga ctggcccaac cggttccggc aagagctcca cgcaggccgc
     3661 catcatcgac atcatcaacg agaagaagta ctaccacatc gtcaccatcg aggatccaat
     3721 cgagtatctt cacttgcaca aacgctcgac gatcaaccag cgtgaagtcg gccacgacac
     3781 gcgagacttt ccttccgccc ttcgcgcgac tctgcgacag gcgccgaaag tgattctcat
     3841 cggcgagatg cgagattttg agactaccga gatcgcgctc gaagcagcgg aaaccggcca
     3901 cctcgtgctt tccacgctcc acacaatcga cgcttcgaag actgtcgacc ggatcatcgg
     3961 actgtatccg aagaacgaag agcccgtgat tcgcacccgt ctggcccaaa cgttccgcta
     4021 catcgtgtcg caacgtctga ttccgcgcgc tgacggcaac ggccgaatcg cggcagtcga
     4081 gattctgcga tcgagcccac gcacgcgcga gtacatcgag gtgggcgaag gagacggcaa
     4141 gtcgctgctc gacgcgatgc gtgacggaaa actcgacggc atgcaggact tcgatagcgt
     4201 gatcaaggga ctgatcgagt cgaaggtggt gtctctcgaa gacggccttg ccttcgcgac
     4261 caaccagaac aatctgctgt tgcagctgaa aggcatgacg gcctcggagg atttcattcg
     4321 tcgcgacggc gtgccgccca cgttttccga ttccggctcg atgctcggca tgattgagta
     4381 gcgcatgatt attgtttgtc ccaagtgttc gacgcgtttg caggtcgacg acgagaagtc
     4441 gccgaatcga ccattcaacg ttcgctgtcc gaaatgcagt gcgactgtga gctccggtcc
     4501 gtcgagtccg gcaactgaac agagcgcact ggctgtcggc ggttctccgt caactgatca
     4561 cccgcgcttt gagccaacga ccgcgcgtgc atacgagcca tcgacgccgg gcgcacctgg
     4621 tgtgacatcg gcgacaccgg caaacgacga cacggttcgc atgttgtttg acatgctctc
     4681 gaaaagcacg aacccgagca gtgagaaccc gaacgcacgt ccctcttggg acaagcgcaa
     4741 ggcgctggtc tgcacgagtg agccttatcg tgagctcgtc gcgagcaagc tctcggaagt
     4801 gggttgccag gttttcgtgg cggaggagac gcgccaggca gttgaaacaa tgcgtgcgaa
     4861 caagatggat gtggtcgtgc tcgagccgca gtttgatcca gccgagcagg gatcggtgtt
     4921 tgtgatgcgc gagatcaacg tgctgcgacc gccgcagcga cgccgcttgt tctttgtgct
     4981 cttgtcgcct tcactgcgca cgatggatgc acacgcagcg tttctaaaca acgtcaacgc
     5041 gatcgtgaat gtcactgaca tcgaagagct gtcgcgcatc atggaggtcg ggctgcgcga
     5101 gtacaacgaa ttgtatcgag agttctacac ttcatttggt ctgacagcct tataagcgcc
     5161 ggatcaactt catctacgag tcatgacttg aaagttgacc cggcgctacc gcgctcggtt
     5221 ctgtgctatc cttctgagcc atctcaatcg aaagcgtgga caggctgttg gggcaacaag
     5281 aacacaccca cgggcaccag cataagaatg gaagctggcg acgactgagt cttgttctgc
     5341 tcctcacgat gctctacatg tttgccgaag tgatcggcgc gtggtggacc ggctcactcg
     5401 cactactcgc cgatgcgggc cacatgttca ccgacgtggc cgcactcatt ctcgcgctca
     5461 ccgccgtttg gtttggctcg cgtccagcca cttcacgcaa gactttcggt tactaccgcc
     5521 tcgaaatcat cgctgcgctc gtcaacggcg tcgcgctcgt cgttatctcg attttcattt
     5581 tttacggggc gtacgaacgc tggcagtcgc cgccagttgt taagactgga ccgatgatcc
     5641 ttgtggcgat tggaggtctg ctcgtgaacc tcgcgtgtgc gtggatactg cactcgcggc
     5701 aggaggtgga tctcaacatg cgcggcgcgt ggatgcacgt gatgggcgac gcgctcgggt
     5761 cggtagcagc gattctggcc ggcgtttgta tggcgttatt cggttggtat gcggctgacg
     5821 cactgttcag tgtggtcatc agtttattga ttatctgggg atcggtgcga ctgatcagag
     5881 agtcgacaaa cgtgctgctg gaaggcactc cggcgcacat caatctcgct gcggttgaga
     5941 atgcgatttt agacacgacc ggtgtctctg acgtacacga tctgcacgtt tggacaatca
     6001 cgtccggacg ggacgcgctg agtgcgcacg tgattcatac tccgagcatt tcgcagcctg
     6061 atctgttgcg agagttgcgc acgaagcttc acgatcgatt tggcgtggac cacctcacga
     6121 ttcagatgga aacccccgat tttgaagacg agacatttca tttctgccac gcgggcacgg
     6181 cgtgtttccg atccgatcag aaccgagcgc ggtgatggaa ttaaatctcc ttttgactct
     6241 cgacctgcga gagcaggcgg ctttgcaggc cgcattgatg acgcacggcg cgcccgactc
     6301 actcgtcacg cttgcgctca cgggcgcgtg tcgcatgccg tcgctcgaag aagcacgaca
     6361 actgcgaaag tggctcgccg aaacacgcac tgccggcgcc actgacttcg ctgcgtttca
     6421 tgtgatcgag aaagcattga tcgattttgg tttataaatc cgtgtattca tccgtgtaat
     6481 ccgtggccca acttgaactg tcatatggac gcaggtctct tgagttcagt tttgatgaag
     6541 cgcgtttctc tgtccttact acggatcgaa ttccaaaaac accgctgaaa gatttcgaga
     6601 tcggactcgc gctcgatgat ccgatcgctt cgccgccaat cgatgaactg gtcagtagtg
     6661 acgactcggt cctgatcgtt gtttctgacg caacgcgcgc caccgccagc gcgcagatcg
     6721 taaacctgct gtcgcgacgt ctcgtccagg ccggagtttc gcccgcaagc atggcggtga
     6781 tctttgcgac cggcattcat cgtccagtca ctgagaaaga aaaggtcgag ctgctgacac
     6841 cgttcatcgt ccagcgtctg cgaatcctga cgcacgatgc gtacgatcag tcaaagctcc
     6901 acgcgtttgg caaaacagag agtggggtca ctgtcgagct caatagcgcg ctgaacgagt
     6961 tctcccgcgt gtttctgacc ggcggtatca cgtttcacta cttcgctgga ttcaccggcg
     7021 gtcgcaaatc gatctgtccg ggactcgcat cggcaaagac gattgaagcc acgcacatgc
     7081 ttgcgttgga tttcgcgagc ggcggtcgca aaacaggtgt acgcgcagga gcgctcgacg
     7141 gcaatgcagt ccatgaagaa tgtgagcgcg ttgcgtcgct tgtcgcgccc acgtttagca
     7201 tcaacacgat cgttaacgag gataagcagg cggtcaggtt atacgcggga gattggcggc
     7261 tggcccatcg cgcagcgtgt gattattatc tcgagcattt ctctgttccc attccgtcga
     7321 aacgcgatat cgtgatcgtc agtaccggtg gctttccgca cgacatcaac ttgatccagg
     7381 cgcacaaggc cctcgacatg gccgcgttgg cctgcaacga tggcggcacg atcatcctgc
     7441 ttgccgaatg cagtgacggg ctcggacggc ccgatttcct gaagtggttc agcaaggctg
     7501 atgcgcgcgc gcttgcggcc cgactggtca acggttatga agtgaatgga caaaccgcgt
     7561 gggcgttgtt gacgaaagca gagcggttcc gtgtttgcct cgtttcggag cttccgcctg
     7621 atgacgtgcg acgcatgcgg atgatcccgg tgcggacgat cgaagaggcg ctcgagcagg
     7681 ccggaaacgg agaagggttc gtcatgccgc gcggtgcggc agtgttgccc ttacatagaa
     7741 gcgtggagac gacgtgaatc tgaggttggc atcgtgtacg gtgcggaact gggagtggcg
     7801 cgaccgcgat gcgattgtgc gctacggaaa caaccgcaaa gtctcgatca acctgcgcga
     7861 tcgttttccg tttccctata ctgcacgcga tgcacgcaac tggctcgaca tggttgtcga
     7921 tgcaaaaccg gtgacgaatt tcgcgattga cgtagccggt gaggcagttg gtggtatagg
     7981 cttcacgccg caacacgacg tcgatcggcg ctctgctgaa atcgggtact ggctgggcga
     8041 gccgttctgg ggccggggga ttgcgaccga agcattgatg gcagtgacgg agtatgcgtt
     8101 tgcgaacttc gacctatgcc gcttgtacgc gcacgtgttt gcctggaacg ccgcttcggc
     8161 gcgagtgctc gaaaaggcgg gatatgaatt tgaaggacgt ttaagaaaga gcgtgacgaa
     8221 ggatggtcag acgatcgacc agcttatgtt tgcaaagatc cgagagtagc cacaaaaggc
     8281 acaacaagca caaaatattt gtttgtgcct tttgtgaact tatgtgcctt ttgtgatctt
     8341 aaaaggagag aaagcatgaa gatgatggca gtcggtttga tgatcgtgtg cagcgcgttg
     8401 ttggcgagtg cgcaggagtt ggccgggggt ttgaatgagc agcgtgtagc tcttacacag
     8461 gcagcggtgg cgttggacgc gagtggtgcg ccggcgctcg aggccacctt gcgaagtact
     8521 gccctaaacg gtgcgccgga aacgcccgtc acgaacgttc gcatcgttgt gaagaatcgc
     8581 agcccgtttc cttacgcgtt cgtctccggc gccgttacgt tttacgacgc ggccggtgtg
     8641 cgttgtggcg agggagtgtt caaggctgac gccatcgccg ttgacgaatc gtttgagagt
     8701 gacttgccgg gactgcggat tcgctgtgaa gccgcgacgt ggcgcatcat cgcgacgagc
     8761 ttgttgccgc gaattcctcc aaacgcacca atcccagccc tcgctcgcac accatcgaat
     8821 ttcataatca gcatcgacgg tgagacgcat ccgattcaac tcgacaaacc actcaccgtc
     8881 acgcttggtg aaaagcgaag aacaataatt gtgcgagctc cctaagaatg ctctcaactt
     8941 taatcgagct tcaacgtctc aaacgcctcg atcgcaccgg ttggactttg cgtggtcttc
     9001 ccaacggcac tgaatccgtc gcggcgcact cgtttggcgt ctgcgtgacc gcgatgttgc
     9061 tcactgattg ttgtaaagcg cgtggcgttg cgatcgacgt cgaaaaagtt ttacgcatcg
     9121 ctttgcttca cgactgggcc gaaacgcgtg ttggagacat gccacggact gctactcttt
     9181 acttcggctc tgcggcgcgc aagcaggctg agactgctgc cttcgcagac atcgttgacc
     9241 cactcgacgc agatagctca tacgcaaatc tctacgacga ctacgaacgt cgcgaaagca
     9301 cagaggcacg gctggtcaaa gctgctgacg tgctcgattt gctgattcag gtgctggcac
     9361 tcgaacgcgc gggcgcccgc ggtctggacg agttctggga agtcgcggaa aacccggaat
     9421 tcaagctcga aggcgttgcg gaacagatag tgctagaact tctgggatcg attctgaaag
     9481 cccgtagcga actgcaacct aatgtttgat ttcttatcgg actttttctt ttccattacc
     9541 tggcgtaaag cactcatcgg agccgcgatt tttcttgtta gtttcttcgc aaacctcggc
     9601 gtcgtcacat tgatcctggt caagttgcgt gccgatcact tcagcaagag tcgcaaaacg
     9661 aaattctggt cgggaccgcg accgtggctt cacgccgcga aagtgatcgg gaaaaacatc
     9721 gccggcgtgt tgctggttgc gctgggaata gtgctgtcgc tgcccggtgt gcccggccaa
     9781 ggtttgctta cagttcttct cggtgtaatg ttgctcgact ttcccggaag acaccggttg
     9841 gaacagaagt tgttaagcag accaagcatt gtgaatgcga tcaacaaatt gcgagaaagg
     9901 ttcgagaaac cgccgcttca gcttaattga atttctcgat tcttcggcgt ttgacgtatg
     9961 attggcgcct aagattgatg ctcaaatact tcaacgccca cagaatcgtg ccctctcgtt
    10021 tactgctgtc agtcttagct gtctcgattc ttttgttcac tgccggctgt ggtttgttcg
    10081 gcgcgaagaa aaagatcacg gtcccgcagc ttctgggtcc gttggcaaaa gccgacaagg
    10141 cgcggttgat tcaggaagtg aaccggctct cgactgttaa atcgattcac ggcaaagttg
    10201 atattcagtt tgaagatacg tcgttcgcgt cgtcaggtat cgccgatcag tatcgactcg
    10261 ttgacggcac gatcacgctg caaagacccg gccagatcta tctgatcatt cagtttactt
    10321 tcgtcgatat cgcacagatg gcgtcagacg gcgagcattt ccgagttgca gttttgaagg
    10381 gtgacgataa atacaagcgg ttcgtgaagg ggactaacag cgccgactat ggaaagtttg
    10441 acgcagacgg cgacgcccag gctcaacaaa ccaaccagca gaagactgag aaggtaacaa
    10501 tcaactcgct ttcaaatcta cgtccgcaac atctgaccga cgcgtttatg attcggccga
    10561 tcgactcgaa tagttcgctg gtttacgcgc agagcgaatt ctttaacgag gaacctgatg
    10621 ttcgtcgtca ggcgaagaag gatgcgcggg tgatgcgagg ttattatttc ctcgatgaat
    10681 tttcgacggc ggctcccggt gacgcaaagc tggtgaggcg tttctggttc gatcgcgtga
    10741 gcggaattcg tcttgcgcgc gtgcaaagtt atgatgagcg aggccagttg atcacggatg
    10801 tgtcttattc taatgaaaag gcgctgggga gtggcccgac cgcttcgttg ccgagccggg
    10861 ttgagattac gcgtccgcag gatcaatata agttgagcat cagttatcag gattctgctt
    10921 cagtcgagct gaatcgcgaa tatccaccac gcgcgtttgt gttggaaaac aagtggggat
    10981 tgccggaagt cgatctcgac tcccagaata agaagacggc caataaataa taattacggt
    11041 atcgatgaga acgattttac aagctgaaga tctggtaaag gtttatcgca tcggaaaagt
    11101 cgatgtgccg gccctgcgtg gtgtttcgct cgaagttgaa gagggcgaat ttgtggcgat
    11161 catggggccg tcgggttgtg ggaagtcgac catgcttcat ttgctgggcg gtttgttgac
    11221 gccgacttcc ggccgcatcg tgatcgatgg tgaagacatc acggccgcct cagacgcgaa
    11281 acggaccgcg attcggcgca aaaagatcgg gtttgtcttt caaagattca atctttttcc
    11341 cacgctgacg gctgaaggaa atctccgact ggcggagcga attcatggca acggcggcgg
    11401 taattcgcag ctcagacgcg aagtgcttcg tctcctggga ctcgaagaca agctgcacca
    11461 caaaccgctc gaattgtcag gcggcgagca gcagcgtgtg gccctggcgc gtgcggtggt
    11521 gacgcgtccg gccattgttc tggctgacga gccgactgga aatctcgatt ccgaaaattc
    11581 agctctcgtc cttaacatgt ttcaggagtt gaattaccgc ttcaaccaga ccatcatcat
    11641 gatcactcac aacccggaag ctgcggctat gtgtaagcgg atcgtacaga tgcgcgacgg
    11701 gcacatcgtc gaacagactc ccaccgtcag gctttaaatg tgtccggaca aactttagtt
    11761 tgtcggcaca actcgacaag ctaaagctta tcggacatca atctgtggac aggactgtta
    11821 ttagaaagct agaatctatt gacccgccag ccacggctcg cgtggctgct tacttgcaag
    11881 cttgaga
//
LOCUS       DASSFQ010000008         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1049179, whole genome shotgun sequence.
ACCESSION   DASSFQ010000008 DASSFQ010000000
VERSION     DASSFQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049179"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFH96_00310"
     CDS             <1..1067
                     /locus_tag="VFH96_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6972427.1"
                     /translation="VSEVVALPQQISRDVAQRLQSRTVNMDHAQLTRNYSPDSEAYRL
                     YLQGRYNWNKRTVEGLTSGIDYFGKAIMRDQDYALAYAGLADCYVLLNVYNVTSADDS
                     CPKADAAARKALSINEDLAEAHTSLGFVSYRYYLKWAEAEEHFKKAIALNPNYATAHQ
                     WYASYLAAVGRLDEAVVDGKTAHELEPFSLTIYSDYIRDLYYAGRLDEARRESLKLVE
                     MDPSFARAHYELGLVLEEEGKLDEAIAEFKLGLKMLPDNIAALTALGHAQARAGRKAE
                     AEKVIERLQELSKQQYVSPFQTAVVYAGLDERKLALDGLEKSRAERFNWLPFIKVDPV
                     LKNLRSEPRFIELAKSLGLP"
     gene            1145..>2319
                     /locus_tag="VFH96_00315"
     CDS             1145..>2319
                     /locus_tag="VFH96_00315"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6972428.1"
                     /translation="MNLSRRNFISSTAKTAVAVTVLRSSVFGQAAAVQPLFARLDEFI
                     ARHMHETGAPGLTLALANREGLIRVSTYGYADTKAGLKVAPETMFEIGSISKSFVGLL
                     LLQLRDEGKLDLNKPIAEYLSWLKINSKFQAITTHHILSHTGGLPGAPLLLDALLGEL
                     WTAWEPGKRFLYSNTGYNILGFLIEAIDKRPFAESMRTRMLVPLGMTASSPIITNDLR
                     KLMAIGYEPLSEGKPFPVNGPLGEAQWLEVDIAAGSVASTPADMAKYMRMMLNRGALP
                     KGRLLSEEGFALFTKAAIDSPYRGEPASYGYGIWVSDIGGHTRLRHTGGMVAFSSSID
                     VDITGNIGAFASVNANLRGYRPVAVTKYAVELFNASAAGKSLPDPPAPPAAPDEVKNA
                     "
BASE COUNT          509 a          581 c          698 g          531 t
ORIGIN      
        1 aggtttccga ggtagttgcg ctgccgcaac agatctcgcg cgacgtcgcg cagcgtttgc
       61 aatcgcgtac ggtgaacatg gaccatgcgc aattgacgcg aaactattcg cccgattccg
      121 aagcgtatcg tctctacttg caaggtcgtt acaactggaa taagcgaacg gttgaaggtt
      181 tgacgagcgg catcgattac ttcggcaagg cgatcatgcg agatcaggat tacgcactcg
      241 cctacgccgg actcgccgat tgttatgtgc tgctgaatgt ctacaacgtc acttcggctg
      301 acgattcttg tccgaaggcc gatgctgctg cgcgaaaagc gttgtcgata aacgaggacc
      361 tggccgaagc acacacatcg ctcgggtttg ttagttatcg ttactacctg aagtgggccg
      421 aggcggagga gcatttcaag aaagcgatcg cgctcaatcc aaactatgcg acggcgcatc
      481 aatggtacgc gagttatctg gcggcggtgg gtcgactcga cgaagcagtc gtcgacggta
      541 agacagccca cgagctggaa ccgttttctc tcactatcta cagcgactac atccgcgatc
      601 tttattacgc cgggcgtctt gacgaagcac ggagggaatc gttaaaactc gtcgagatgg
      661 atcccagctt cgcgcgtgct cactatgaat tgggcctggt gctcgaggaa gaagggaagc
      721 tcgacgaagc aattgccgaa ttcaaactcg gattgaagat gttgcctgac aacatcgcgg
      781 cgctgactgc gctggggcac gcgcaggctc gagcgggtag aaaggccgag gcggaaaaag
      841 tgatcgagag actgcaggag ctttcgaagc agcagtacgt ttcgcctttt cagacggcgg
      901 tggtttatgc agggctcgac gaacgcaagc tggcgcttga cgggttggag aagtcgcgcg
      961 cagagcgttt caactggttg ccgtttatta aagtcgatcc ggtgttgaag aatttgcgtt
     1021 cggagccgcg atttatcgag ttagctaaga gtttaggttt gccgtaagaa tttctcgcgc
     1081 aaaggcgcaa aggcgcaaag gaagacttct ttgcgtcttt gcgcctttgc gggagaaatc
     1141 atctatgaat ctttcacggc gtaactttat ttcgagcact gcgaaaactg cggttgctgt
     1201 cactgttctg cggtcgagtg tgtttgggca ggcggcggcg gtccagccgt tgtttgcgcg
     1261 gctcgatgaa tttattgcgc gtcatatgca cgagacgggc gcgccggggc tgacgctcgc
     1321 gctggcgaat cgtgagggat tgattcgtgt ttcgacttac gggtatgcgg acacaaaggc
     1381 gggtttgaag gtggcgccgg aaacgatgtt tgagatcgga tcgatctcga aatcgtttgt
     1441 cggtctgctg cttttgcagt tacgcgacga aggaaaactc gatctcaaca aaccgattgc
     1501 tgagtatctg tcgtggctca agatcaattc gaagtttcag gccatcacga ctcatcacat
     1561 actgagtcac actggaggac ttccgggcgc gccgctgttg ctcgatgcgt tgcttggcga
     1621 gctctggacc gcgtgggaac ccggaaaacg attcctttat tcaaacactg gctacaatat
     1681 tctcggtttc ttaatcgaag cgatcgataa gcgcccgttt gccgaatcga tgcgcacgcg
     1741 catgctggtt ccgctcggca tgactgccag ttcgccgatc attacgaatg atctgcgcaa
     1801 gctgatggct atcggttacg aaccactttc cgaaggcaag ccatttccgg tgaatgggcc
     1861 actcggcgaa gcgcaatggc ttgaagtcga tattgctgcc ggaagcgtcg cttcaacgcc
     1921 agctgatatg gcgaagtaca tgcgcatgat gctcaatcga ggtgcgttac caaaaggccg
     1981 gctgctttcg gaagaagggt ttgcgctctt cacgaaggcg gcgatcgatt caccgtatcg
     2041 cggcgagccg gcttcgtatg gttacgggat ttgggtgagc gacattggtg gtcacacgcg
     2101 cctgcgtcac acgggtggaa tggtcgcgtt cagctcgtca atcgacgttg acatcacggg
     2161 taacatcggt gcgtttgcat ctgtaaatgc aaacctgcgc ggctatcgac cggtggcggt
     2221 gacgaaatac gcagtcgagc tcttcaacgc gtccgcagca gggaaatcgc ttcccgatcc
     2281 gccggcgcca ccggccgctc cggatgaagt gaagaacgc
//
LOCUS       DASSFQ010000009         5339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1795059, whole genome shotgun sequence.
ACCESSION   DASSFQ010000009 DASSFQ010000000
VERSION     DASSFQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795059"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFH96_00320"
     CDS             <1..295
                     /locus_tag="VFH96_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972429.1"
                     /translation="LREVVEPIFLKYGVDVVLTGHDHVYERMKPQKGIYYFVSGAGGK
                     LRTGDIDRQSGLTEKSYDRDLHFMLFEVAGDQMYFQAISRTGETIDSGVINRK"
     gene            complement(329..3106)
                     /locus_tag="VFH96_00325"
     CDS             complement(329..3106)
                     /locus_tag="VFH96_00325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6972430.1"
                     /translation="MKPDRWRKVDELFEAALDREPSHRAAFLDEACGSDRELRREVEK
                     MLDIDNQAQDFIKTDVFNVAAKLITERTPKKSIFTSDSIDDARFVPGDILSERYRIVG
                     LLGRGGMGEVYRADDLKLKQAVALKFLPASLNTDGAALARFYKEVSVARQISHRHVCR
                     VYDVAEYNGEHFISMEFVRGEELSSLLKRIGRVPQDKAVDVARQLCAGLAAVHERGVL
                     HRDLKPANIMLDEHGEVRITDFGIAALAVEDPREMSGTPAYMSPEQLDSQELTARSDI
                     YSLGLVLYELFTGKKAFQATSLPELLRLRRSDTTPTSPAEHVPDLDPLIERVIFRCLE
                     RDPSKRPASALQVAAALPGGDPLTAALAAGETPSPEMVAAAPKLGALKPAVAMSLLAA
                     VVVLLGTICFLSRYTTLYRRVPLNKSPEVLRDRAAELVRKFGYTTPAGDSAYGMGLDR
                     DYLDYIHDNDQSPTRWNRLSSGQPAAIYFWYRQSPRPFSMSTDGNISEEYPVRDFPGM
                     TTLTLDTLGRLRSFYVVPPAKSSGSDAATPDWSPLFIEGGLDQTKFQSVSPVWTPPHE
                     ATLRAAWEGSYPDQPDLKIHIEAGAFEGKPVYFEIFDAWDQPREAQVSVARFRQRALA
                     VLLLTIFITVMIGSGLLALRNVRLGRGDRKGAFRLAGFLVVVFSLRWLFSSHHVATEA
                     EAFNFITGVQTILYWTFFFWVVYLAFEPFVRRRWPGRIVSWSRLLAGGFRDPLVGRDI
                     LIGAVFGLGIILSNFYLASLAPQWLGYPPAIPWMDFPATQLLGIRSFPFGITQQIFGA
                     LFQSFILLFFLLLLYIILRRERLAAVAVWLIGSVALSLTHETPLGVPFSCVAVFLLVW
                     VLYRYGLLALVSTIFFLHLVIFFPITSDFTAWYAADFVLALIVSLTIAGFAFYTSLAG
                     QPLFRASLPDD"
     gene            complement(3075..3530)
                     /locus_tag="VFH96_00330"
     CDS             complement(3075..3530)
                     /locus_tag="VFH96_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HET6972431.1"
                     /translation="MRCRQPHVNEAYLKLAGQQGFDWQNRAHFFAVCAQVMRHILIDH
                     ARAHARDKRGGGAVKVSLNDALVVAEDQASHFIALDEALRALESLDPQKGKIVELRYF
                     GGLSVEEAAEVMNISPRTVRREWQRAKAWLYRMMTEGIEDETRPLAKSR"
     gene            3774..4232
                     /locus_tag="VFH96_00335"
     CDS             3774..4232
                     /locus_tag="VFH96_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HET6972432.1"
                     /translation="MKTLVFVTLLMMGFSGQTMHQDSRAADEAAMRESVKQLETGWNT
                     KSGALFAKPFAEDADYVVINGNYIKGRAVIESQHQRIFDTIFKDTNITLTVKQIRFLR
                     ADVAVVHVEGHRTSTTQALVQDAMLTLMMTKEKQGWVIAAFQNTAVAPRG"
     gene            complement(4324..4716)
                     /locus_tag="VFH96_00340"
     CDS             complement(4324..4716)
                     /locus_tag="VFH96_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6972433.1"
                     /translation="MSNLKSVKTVYEAFATGDIFTVLGTLSPDIAWTEAEGFPYGGTY
                     HGPKAVLEGVFMRLGSEWEGFAAVADEFIDGGDTVVVLGKYSGKYKKTGKSFQANFAH
                     VWKMQDGKAAQFIQHVDTLLVHRALQAD"
     gene            4812..>5339
                     /locus_tag="VFH96_00345"
     CDS             4812..>5339
                     /locus_tag="VFH96_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6972434.1"
                     /translation="MKDVDDIVRRAADPSSAPEARHEAFGELVIRFQDMAFACAFAVL
                     GDACLAQDTAQEAFVTAWQKLAQLREPAAFPGWFKRIVLTQCNRLTRCKRLQILPLEA
                     VDKPTDDPGPQTSAEQRDLITKVLEAINALPENERLVTTLFYVNGYTQADIGDFLEVP
                     VSTVNKRLYSARQRLK"
BASE COUNT         1213 a         1441 c         1569 g         1116 t
ORIGIN      
        1 actgcgagaa gtggttgagc cgatctttct caagtacggc gttgatgtgg tgctgaccgg
       61 gcacgatcac gtctacgagc gaatgaagcc gcagaagggg atttactatt ttgtttccgg
      121 ggcaggtggg aagctgcgca ccggtgatat cgacaggcag tcagggctca cggagaagtc
      181 gtacgatcgc gacctgcact tcatgttgtt tgaagtagcg ggtgatcaga tgtacttcca
      241 ggcgatctcg agaacgggtg agacgattga ttcaggtgtt ataaaccgaa agtaatttag
      301 cgcggattat acggattaaa tacacggatt agtcgtcagg caatgaggcg cggaagagtg
      361 gttggcctgc gagtgaagtg tagaaagcaa agccggcgat tgtgagtgag acgattagcg
      421 cgagtacgaa atcagccgcg taccacgccg tgaaatcaga agtgatcggg aaaaagatca
      481 ccaggtgcaa aaagaagatc gttgagacca gcgccagcaa accgtaacga taaagcaccc
      541 ataccaacag aaacaccgcc acacaagaga agggcacacc gagaggagtc tcatgcgtga
      601 ggcttaaagc gacgctccca atcaaccaaa ccgcgactgc cgccagtcgt tcgcgacgca
      661 ggatgatgta aagcaacagc agaaagaaca gcagtatgaa tgattgaaaa agcgcgccga
      721 agatctgttg cgtgatcccg aaaggaaacg aacgaatgcc gagtagttgt gtagccggaa
      781 aatccatcca cggaatcgcg ggcggataac caagccattg cggcgccaga ctggccagat
      841 aaaaattgga gagaatgatt cccagaccaa agaccgcgcc gatcagtatg tcgcgtccga
      901 cgaggggatc gcgaaaacct ccagccagca ggcggctcca cgaaactatc cgtccgggcc
      961 agcgtcgtcg cacaaacggc tcaaacgcga gatagaccac ccagaagaag aacgtccagt
     1021 agagaatcgt ctgcacgccc gtgatgaagt tgaaggcttc ggcctccgtc gccacgtgat
     1081 gtgacgagaa gagccatcgc aaagagaaca ccaccaccag aaaaccggcg agacgaaacg
     1141 ctcctttgcg atcaccgcgc ccgagtctca cgtttcgcag cgccagcagc ccgctaccaa
     1201 tcatcacggt gatgaagatc gttagcagca aaaccgccaa cgcgcgttgc cggaaacgcg
     1261 cgactgaaac ttgcgcctcg cgtggctggt cccatgcgtc aaagatctcg aagtaaacag
     1321 gcttgccctc aaacgcaccg gcttcgatgt gtatctttaa gtctggctgg tcgggatagg
     1381 agccttccca cgctgctcgt aaggttgctt cgtgtggcgg agtccaaaca ggtgacacag
     1441 attggaattt ggtttggtcc aacccgccct cgatgaaaag cggcgaccag tcgggtgtgg
     1501 cggcatcaga accagaactc ttcgcgggtg gtacgacgta gaaagaacgc aaacgtccca
     1561 gtgtgtcgag ggtcagcgtg gtcatgccgg gaaagtcgcg cactgggtac tcttcgctaa
     1621 tgttgccgtc ggtgctcatc gagaatggac gtggactctg acggtaccag aaatagattg
     1681 ctgccggttg tccgctgctt agtctgttcc atcgcgttgg cgattgatcg ttgtcgtgga
     1741 tgtagtcgag gtagtcgcga tcgagtccca tgccgtaagc agaatcgccg gccggcgtcg
     1801 tgtacccaaa ttttctgacc agctcagccg cacgatcgcg taaaacttcc ggcgacttgt
     1861 tcagcggaac gcgccgatac aacgtcgtgt agcgcgacag aaaacagatc gtgccgagca
     1921 gcacgacgac cgccgcgagc aacgacatcg cgaccgcggg tttcaatgcg cctagcttcg
     1981 gcgctgcagc aaccatctca ggcgacggcg tctcacctgc tgcgagcgcg gccgtgagtg
     2041 gatcgccgcc cggcaaagca gccgcgactt gcaacgctga cgccggacgc ttcgaaggat
     2101 ctctttcgag acaccggaag atcacgcgct cgatcagcgg atcgagatcc ggcacgtgtt
     2161 ccgccggact cgtcggcgtc gtatcgcttc gtcgcaaacg aagtagctca ggcaagctcg
     2221 ttgcctggaa cgctttcttg ccggtgaaca gctcgtagag cacgagaccg agcgagtaga
     2281 tgtcgctcct cgctgttaac tcctggctgt cgagttgttc gggagacatg tacgcgggcg
     2341 tgcccgacat ctcgcgcgga tcttccaccg cgagtgcggc gataccgaaa tcggtgatgc
     2401 gcacctcgcc gtgctcgtcg agcatgatgt tcgcgggttt cagatcgcga tgaagcacac
     2461 cgcgctcgtg taccgcggcg agtccggcgc agagttggcg tgcgacgtcg acggctttgt
     2521 cctgcggcac gcgtccgatg cgtttcagga gcgatgagag ctcctcgcca cgcacaaact
     2581 ccatcgagat gaaatgttct ccgttgtatt cggcgacgtc gtaaacacga caaacatgtc
     2641 gatgtgagat ctggcgcgcg acgctaactt ctttgtagaa acgcgcgagc gccgcgccgt
     2701 cagtgttcag tgaagcaggc agaaacttga gcgcgaccgc ttgtttgagt ttgagatcgt
     2761 cggcgcgata aacctcaccc atgccgccgc ggccgagtag tccgacgatg cgataacgtt
     2821 cggaaagaat atcgccggga acgaatcgcg cgtcgtcaat cgagtctgaa gtgaagatcg
     2881 actttttagg cgtgcgctct gtgatcaact tcgcagcgac gttgaagaca tccgttttga
     2941 tgaagtcctg cgcctggttg tcgatgtcga gcatcttctc gacttcgcga cgcagctctc
     3001 gatcgctgcc gcaagcctcg tcgagaaacg cagcgcgatg tgaaggctcg cgatcgagcg
     3061 cggcctcaaa gagctcatcg acttttcgcc agcggtcggg tttcatcttc gattccttcg
     3121 gtcatcatgc gatagagcca cgccttcgct cgctgccact cgcgtcgcac tgtccgcggt
     3181 gaaatgttca tcacttccgc tgcttcctcg acgctcaacc cgccgaagta acgtaactcg
     3241 acgatcttgc ctttctgtgg atcgagactc tcgagcgcac gcagcgcttc atcgagagca
     3301 atgaagtgag acgcctgatc ctccgctact actaaagcgt cattcagcga aactttgacc
     3361 gcaccgccgc cgcgtttgtc gcgggcgtgg gcgcgagcgt gatcgatcag gatgtgtcgc
     3421 atcacttgcg cacagactgc gaagaagtgc gcgcggtttt gccagtcgaa tccctgctga
     3481 ccggcgagtt tcagatacgc ctcgttgacg tgcggttgcc tgcaacgtat gaccggggcg
     3541 ctcctgactc atctggcgtt gagcgatgcg ccggagctcc gagtgtatga gcgggaagag
     3601 cttgtcgagc gcagaccggt cgcctttacc ccaatcggcg agtagttggg tgacttcctg
     3661 cggcgagggc gtgggcatcg gtcgcggatt ctatcatgcc tatatgggtg gccgcttttg
     3721 ttttcattcg tcgcttacag gtatggcaga ggttgatact ttaggaggac gtgatgaaaa
     3781 cactcgtatt tgtgacgttg ttgatgatgg gtttctcagg gcagacgatg catcaggaca
     3841 gtcgcgcggc ggacgaagcg gcgatgcgcg aaagcgtgaa gcagttggag accggttgga
     3901 acacgaaaag cggcgcgctc ttcgcgaagc cgttcgccga ggatgcggat tacgtggtca
     3961 ttaacggtaa ttacatcaag ggacgtgcgg tgatcgagag tcagcaccag aggatctttg
     4021 acacgatatt taaggacacg aatatcacgc tgacggtcaa acagatccgg ttcctgcgcg
     4081 ctgatgttgc ggttgtgcat gttgagggac accggacgag taccactcaa gcgctcgtgc
     4141 aggatgcgat gttgacgctg atgatgacga aggaaaagca gggctgggtg atcgctgctt
     4201 tccaaaatac agccgtggcc ccaagaggat gattagccac aaaaggcaca aaaagcacaa
     4261 aagataaaaa caagccccgt ttctgacttg ttttctcttt tgtgcttttt gtgccttttg
     4321 tggctaatct gcttgaaggg ctcggtgaac cagtaacgtg tcgacgtgct ggatgaactg
     4381 ggccgctttg ccgtcctgca ttttccagac gtgcgcgaag tttgcctgaa agctcttgcc
     4441 tgtcttcttg tacttgccgc tgtacttgcc taacacgaca acggtgtcgc cgccatcgat
     4501 gaattcgtca gccacggcgg cgaagccttc ccactccgag ccgagacgca tgaacacgcc
     4561 ttcgagcacg gcctttggac catgataagt gccgccatat ggaaaacctt cggcttcggt
     4621 ccaggcgatg tcggggctta gagttccgag gacggtgaaa atatctccgg tagcgaacgc
     4681 ttcgtaaacc gttttgactg atttgagatt gctcaagtgt tttcctcttc gtgaaaattt
     4741 ggcggcccat cttacaccaa ccttctgtga ctggtaagct ttgtcgtaga ttctggctct
     4801 tattaataga agtgaaagac gttgacgata tcgttcgccg cgcggccgat ccgagtagcg
     4861 ctccagaggc acgacacgaa gcctttggcg agcttgtgat ccgctttcag gacatggcgt
     4921 tcgcttgtgc gtttgccgtg ctcggcgacg cctgcctcgc acaggacacc gcccaggaag
     4981 cgttcgtcac tgcgtggcag aagctcgctc aactgcgcga gccggcggcg tttcccggtt
     5041 ggttcaagcg gatcgtcttg acgcagtgca atcgtttgac gcgttgcaag cgcttgcaga
     5101 tcctgccgct cgaggctgtt gataagccca cagacgatcc gggaccacaa acgtccgcgg
     5161 aacaacgcga cctcataacg aaagtgctgg aggctattaa cgcactacca gagaatgaac
     5221 gactggtgac gactctgttt tacgtcaacg gttacaccca ggccgatatc ggtgactttc
     5281 tcgaggtgcc ggtctcgaca gtcaacaagc gtctgtattc agcgcggcaa cgattgaag
//
LOCUS       DASSFQ010000010         5827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1199786, whole genome shotgun sequence.
ACCESSION   DASSFQ010000010 DASSFQ010000000
VERSION     DASSFQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199786"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFH96_00350"
     CDS             <1..377
                     /locus_tag="VFH96_00350"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HET6972435.1"
                     /translation="RKLPDETVAAADALINQIADDSALQARVAEVLSGESGTEIWSLV
                     MLSQQLEEVVGQCRNSAEPANLAKYTFSLARQFSRFYHDHRIITEQDEVRRGVLIAVT
                     KIVRDRMIAALGILGITVPEQM"
     gene            403..1419
                     /gene="aroF"
                     /locus_tag="VFH96_00355"
     CDS             403..1419
                     /gene="aroF"
                     /locus_tag="VFH96_00355"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6972436.1"
                     /translation="MLIVMKSDATDSQIDDVLRVIEALGFKGHPMPGATRTAIGITGN
                     QGAVDPSHFENLTGVAEAIRVSKPYKLITLDLRPEKTIVRVGDATIGGDELAIIAGPC
                     AIESRNQAFAIAEAVRRSGARFFRGGAYKPRTSPYAFQGLGEEGLRILAEVRDTYGLK
                     IVTEALDEAGVDLVERYGDMIQIGARNMQNFSLLRRAGKAKLPVLLKRGLSATLDEWL
                     LAAEYIMAEGNYQIVLCERGIRTFAQHTRNTLDLAAIPAVRRISHLPVLVDPSHGTGK
                     NFMVTPLARAGVAVGADGLIVEVHDQPEHALSDGAQALTLEQYEQLVSEVRSIHEVIA
                     PVSV"
     gene            complement(1389..1775)
                     /locus_tag="VFH96_00360"
     CDS             complement(1389..1775)
                     /locus_tag="VFH96_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972437.1"
                     /translation="MSVIVISVVDDMFFASKIRAVAEAAGVEISFPRGQEAVVAKARE
                     TKPGLIVVDLHNQKIDPVALARELKADDELRRIRLLGFFSHVQTELQRNALAAGFDQV
                     VPRSVFARDLPEILATDYTDTGAITS"
     gene            1774..2610
                     /locus_tag="VFH96_00365"
     CDS             1774..2610
                     /locus_tag="VFH96_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6972438.1"
                     /translation="MIIASYNIRYARGPYLISGGLRRKMGLMSLTRRPEHVARQISAA
                     ARAFSEGKLLPRVDVLALQEADMRTKRTGGHHVAEELAAELGMHWAHEAAGIPRGVKP
                     VKRQWWLDFEEPIDLHDSGDTGVALLSRLPLTNVTRIDLPWQECQWRPRLAIAATIKV
                     GTQDVRIFNAHVDPHAAVDGQLAQLEAIATQTEATTLPTVVLGDFNTLSKQKCIETRE
                     FLEARGFTTPFRTGTPTWRTTVLKMHADWIFVRGVKIERWGVAKPLNVSDHWPIWAEV
                     AV"
     gene            2610..3410
                     /locus_tag="VFH96_00370"
     CDS             2610..3410
                     /locus_tag="VFH96_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6972439.1"
                     /translation="MHITSRDNSLLREARSVRDGKIDDLIFVEGLRLCEEARRSNLEI
                     EAVIVSEELMRKERAAQAIAELSRVSKRTASVSEKLLESISYTKTPQGIVVLAGRPDS
                     SEERLTASLAASPLLVVLHQINNPVNVGAILRTAEAAGATGVIATRNTSDPFSPKSLR
                     GAMGSAFRLPIWTGPSYEEAVDWCRTRSIITVCSDAEADADYTDLDWTNATALLLGPE
                     STGFTAEELVYADRTVKITMRGKTESLNVSVAAGIFLFEAARQRAIAP"
     gene            complement(3397..4308)
                     /locus_tag="VFH96_00375"
     CDS             complement(3397..4308)
                     /locus_tag="VFH96_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET6972440.1"
                     /translation="MNGSRREFLQAACAGLGVTLLDRNISPEDFENVFKEIRKNILEM
                     INEERDVEKVPLVEMDDLATEVANKHAVDLVTNQFIGHWGSDGRKPYHRYSAAGGTHA
                     TEENVSAADGIRSTTEKDLKQDSAYLHVRMYQEKPPNDGHRRTILAPQHTHVGIGFAV
                     QGLRLRMVQLFVAKYVDVKPIKREARPGAQLSFSGKIVRQGYLFNNIEVFYEPLPTPP
                     ELEWLRQPRSYALPKESRVLKPRVPPPFTYTDGTTGIVEVYADKSFQTPVSLYKNKPG
                     IYTIVVWLRTAQSRVAFPATAVCIKAQ"
     gene            complement(4305..4919)
                     /gene="can"
                     /locus_tag="VFH96_00380"
     CDS             complement(4305..4919)
                     /gene="can"
                     /locus_tag="VFH96_00380"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HET6972441.1"
                     /translation="MKDLHRLLDQNRAWAENIKASEPDFFQNLAKQQSPRFLWIGCAD
                     SRVPSTQLAGMKPGDMFVHRNVANLVVHTDFNCLSVMQYAVDVLKVEHIIVCGHDGCG
                     GVKAAMSNLQFGLIDNWLRHVQDVFHEHEEQLAKISDENERVDRLCELNVIEQVRNVA
                     RTTIVQSAWQRGQDLVVHGWIYGLQNGLLRDLGVSIDNADERGL"
     gene            complement(4998..>5827)
                     /locus_tag="VFH96_00385"
     CDS             complement(4998..>5827)
                     /locus_tag="VFH96_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972442.1"
                     /translation="VLSHSTIAPAVVDPLLIKQLSDVLLPKSPIHFPLLSSHPQKKAL
                     APLIGLETGSIRMAKQIMSSKGVPFSIEDWPSVVLEGLRVLNENNWFPAMTLIVGNPG
                     ETDQDVRETIDLVYEIERRGLFAFLIPSIFTPLHDTRMEKMEGVTQTRQLTPLQWQLM
                     MKCWKMNLRPGQYSWWGPMAWRVGAIGMWLYKLRRLNGPNFTWPLWMFSGAISEKNLQ
                     RMGKIYRGKPLKVKTRAELIASLKPKDWQHLRADNGDMPLGYAPPLAAHAVASLART"
BASE COUNT         1270 a         1634 c         1638 g         1285 t
ORIGIN      
        1 ttcgcaagct gccggacgaa actgtcgctg ccgcggatgc gttaataaat cagatagctg
       61 atgacagcgc cttacaagca agagttgcgg aggtgttgtc aggtgaaagc ggcacggaga
      121 tctggtcgct cgtgatgttg tcgcaacagc ttgaagaggt cgttgggcag tgtcgcaatt
      181 ctgcggagcc tgctaatctt gccaagtaca ctttctcgct tgcccgacag tttagccgtt
      241 tctatcacga tcaccggatc attactgagc aggatgaagt gcgaagaggt gtgctaatcg
      301 cggtgacgaa gatcgttcga gatcgaatga tcgctgcctt gggaatactt ggaatcactg
      361 ttcccgagca gatgtaagta agacgcaaag aaggaaaaca ccatgttaat cgtaatgaaa
      421 tctgacgcga ctgacagtca aatcgatgat gtcctgcgcg tgatcgaggc actcggcttc
      481 aagggccatc cgatgcccgg cgccactcgc accgccatcg gcatcaccgg caaccagggc
      541 gcggtcgatc cgtctcactt cgagaacctc accggtgtcg ccgaagccat tcgcgtcagc
      601 aagccttaca aactgatcac gctcgacctg agaccggaaa agacgatcgt gcgcgtaggc
      661 gatgcaacga tcggcggcga cgagctcgcg atcatcgctg gtccatgcgc gattgagagt
      721 cgcaaccagg cgttcgcgat tgccgaagca gtcagacgca gtggcgctcg tttcttccgc
      781 ggcggcgctt acaagccgcg cacgtcacct tatgctttcc agggactggg cgaagaaggt
      841 ttgcgaatac tcgcggaagt ccgcgacacg tacgggctca agatcgttac cgaagcactc
      901 gatgaagcag gcgtggatct ggtggagcgt tacggcgata tgatccagat cggcgcgcgc
      961 aacatgcaga acttctcgct gctgcgacgg gcaggcaaag cgaaactacc ggtactgctc
     1021 aagcgtggac tatcggccac gctcgacgag tggctgctcg ccgcggagta catcatggcc
     1081 gagggcaact accagatcgt tctgtgtgag cgcggcattc gcacctttgc gcagcacacg
     1141 cgcaacactt tggaccttgc ggcgattcca gcggtgcgcc gcatctcgca tcttcccgtg
     1201 ctcgtcgatc cgtcacacgg gaccggcaag aattttatgg tgacgccgct cgcgcgtgca
     1261 ggtgtcgcgg ttggcgcgga cggtttgatt gtcgaagttc acgatcaacc agaacacgcg
     1321 ctctcagacg gtgcccaggc actgacactc gaacagtacg aacagttagt cagcgaggtg
     1381 cgctcaattc acgaagtaat cgcgcctgta tccgtgtaat ccgtggctaa tatttctgga
     1441 aggtctctcg caaacacgga tcttggtaca acttgatcga atccggctgc gagcgcgttg
     1501 cgttgcaact cagtctgaac gtgcgaaaag aacccgagca acctgatgcg ccgcagctcg
     1561 tcatcggctt tcaactcccg agccaatgcc accggatcga tcttttgatt gtgcagatcc
     1621 acaactatta accccggctt cgtctcgcgt gcctttgcaa ccacagcctc ctggccccgc
     1681 ggaaaactta tctccacacc tgccgcttcc gcgaccgcgc gaatcttcga cgcaaagaac
     1741 atatcgtcaa caactgatat aactatcaca ctcatgataa tcgcatcata caacatccgt
     1801 tacgcgcgcg gtccctatct gatctccggc gggcttcgtc gcaaaatggg attgatgagt
     1861 ttgacgcgac gacccgaaca tgtggcgcgc caaatctctg cagccgcacg cgcattcagc
     1921 gaaggaaaac tgctgccacg cgtcgacgtg ctcgctctgc aggaagctga catgcgtaca
     1981 aagcgaaccg gcggtcatca cgttgccgaa gagctcgccg cagagctcgg aatgcactgg
     2041 gcccacgaag cggctggaat tccgcgcggt gtgaaaccgg tcaagcgaca atggtggctc
     2101 gactttgaag agccaattga cctgcatgat agtggcgaca cgggcgtcgc gctgttgagt
     2161 cgtctgccgt taacgaacgt gacgcggatc gatctgccgt ggcaggagtg tcaatggcgt
     2221 ccacgactcg cgattgcggc gaccataaag gttgggacgc aggacgttcg gattttcaac
     2281 gcccacgtcg atccacacgc cgcggtcgat gggcagctcg cgcaactcga ggcgattgct
     2341 acacaaaccg aggcgacaac cttgccgacg gtcgtgcttg gtgatttcaa caccctctca
     2401 aaacagaagt gcatcgagac gcgtgagttt ctcgaggcgc gcgggtttac gacgcctttt
     2461 cggaccggaa ccccgacctg gcgtaccact gttttgaaaa tgcatgccga ctggatcttc
     2521 gtgcgcggag ttaagattga gcgctggggc gtggcgaagc cattgaacgt ttcggatcac
     2581 tggcccatct gggccgaagt cgcagtttga tgcatataac gagtcgtgac aattccctgc
     2641 tccgcgaggc gcgttcagtt cgcgatggaa agatcgacga tctgatcttt gtcgaggggc
     2701 tgcggctgtg tgaagaggcg cggcgctcga atttagagat cgaggccgta attgtttctg
     2761 aagagctgat gcgaaaggaa cgcgcggcgc aagcaatcgc cgagctttcg cgcgtttcaa
     2821 agcgaacggc ttcagtcagt gagaaactgc tcgaatcgat ctcctacacg aagacaccgc
     2881 agggtatcgt cgttctcgcc ggcaggcctg actcctccga ggaacgtcta accgccagtc
     2941 tcgccgcgag tccgctgctc gtcgtgctgc atcagatcaa caatcccgtg aatgtcggcg
     3001 cgatcctgcg caccgccgaa gcagcaggcg caactggcgt tattgcgacg agaaacacga
     3061 gcgatccttt ctcgccgaag tctttgcgtg gagcgatggg atcggcgttc aggcttccga
     3121 tctggaccgg tccatcgtat gaagaggctg ttgactggtg tcgcacacgt agcatcataa
     3181 ctgtctgttc cgacgcagaa gcagacgcgg attacacgga tctggattgg accaacgcca
     3241 ctgcgttgct gcttggacca gagtcgaccg gtttcactgc tgaagaattg gtttacgcgg
     3301 atcgtaccgt gaagatcacg atgcgaggaa aaacggagag tctcaacgtc tcagtcgctg
     3361 ccggtatttt cctgttcgag gccgctcgtc aaagagctat tgcgccttga tgcacacggc
     3421 ggttgcggga aaagcgactc tggattgggc ggtcctgagc caaaccacga tcgtgtagat
     3481 tcccggcttg ttcttgtaca gactgaccgg cgtttgaaaa ctcttatccg cgtagacttc
     3541 aactattccc gttgtcccgt ccgtataagt aaacggcggt ggcactcgcg gtttcagcac
     3601 tctcgactcc ttcggcaacg catacgatcg tggttgccgc agccactcca gctccggcgg
     3661 agtcggcaaa ggctcgtaaa atacttcgat gttattaaac aagtaaccct gtcgcactat
     3721 cttcccgctg aaagaaagct gggcgccggg ccttgcctcc cgtttaatcg gcttgacgtc
     3781 aacatacttc gccacaaaca actggaccat ccgcagtctt agaccttgca cggcaaagcc
     3841 aatcccgacg tgcgtgtgtt gtggcgcgag gatggtgcgg cgatgtccgt cgttcggtgg
     3901 cttttcctgg tacatccgca cgtggagata agcactgtcc tgcttcaggt ctttctctgt
     3961 tgtggacctt atgccatcgg cggcggaaac attttcttcg gtcgcgtgtg taccgccggc
     4021 ggcagaataa cgatggtaag gtttgcggcc atcactgccc cagtgaccga taaactggtt
     4081 ggtgacgagg tcgacggcgt gtttattagc aacctcagtc gcaagatcgt ccatctccac
     4141 gagcggaact ttctcaacgt cgcgctcctc gttgatcatt tcgagaatat tcttgcgaat
     4201 ctctttgaag acgttttcga aatcctctgg tgagatgttg cggtcgagta acgtgacccc
     4261 aagcccggca caagcagctt gcagaaattc acgtctcgat ccgttcataa tccgcgttca
     4321 tccgcgttat cgattgagac accgagatcg cgcagcagcc cgttctgcag tccgtagatc
     4381 caaccgtgta cgaccagatc ctgtccgcgt tgccaggcgc tctgcacgat cgtcgtgcga
     4441 gcaacgttcc gcacttgttc gatgacgttc agctcacaca agcgatcgac ccgctcgttt
     4501 tcatcggaga tttttgcgag ttgctcttcg tgttcgtgaa agacgtcctg tacgtgacgc
     4561 agccagttgt cgatcaggcc gaactggaga ttactcatcg ctgccttcac gccgccacag
     4621 ccgtcatgac cgcagacgat gatgtgctcc actttcaaca cgtcgaccgc atattgcatc
     4681 accgacaaac agttgaagtc ggtatggacc acgaggttcg cgacgttgcg gtgtacgaac
     4741 atatcaccgg gtttcatgcc ggcgagttgt gtggaaggca cgcggctatc agcgcagccg
     4801 atccagagga aacgtggtga ttgttgtttg gctaagtttt gaaaaaagtc aggctcgctg
     4861 gctttgatgt tttcagccca ggctcggttt tgatcgagaa gacgatgaag atctttcata
     4921 catttttaga caggattgac aggatttgca ggattgacaa gatgcatcat gaagtcttga
     4981 aaatcctgta atcctgtcta cgttcgtgct aaagaagcga cggcatgtgc agctaacggt
     5041 ggcgcatatc ccaagggcat gtcgccgttg tctgcgcgca gatgctgcca gtctttcggt
     5101 ttgagcgagg cgatcagctc cgcgcgagtc ttcaccttca agggcttgcc gcgatagatc
     5161 ttgcccatgc gttgcaggtt cttctcggag atcgcgccgg agaacatcca caagggccac
     5221 gtgaagtttg gaccattaag gcgccgcagt ttatacagcc acataccgat cgcgcccacg
     5281 cgccaagcca tcggtcccca ccaactgtac tggcctggcc gcaggttcat cttccagcac
     5341 ttcatcatca actgccactg taacggtgtg agctggcgcg tttgcgtcac gccctccatt
     5401 ttttccatgc gcgtgtcgtg gagtggagtg aagatcgatg ggatcaggaa cgcaaacaga
     5461 ccacgacgct cgatttcgta aacgaggtcg atcgtctccc tcacgtcttg atcagtctcg
     5521 ccgggattgc cgacgatcaa cgtcatcgcc gggaaccagt tgttctcgtt cagcacgcgc
     5581 agtccctcga gcacgacgct gggccagtct tcgatcgaaa acggcacgcc cttgctcgac
     5641 ataatctgct tcgccatgcg gatcgagccg gtttccaaac cgatcaaagg cgcgagcgct
     5701 tttttctgcg gatgactgct caggagagga aaatgaatcg ggctcttagg cagcaggacg
     5761 tccgaaagtt gtttgatgag taaagggtcg acaacggccg gcgcgatcgt gctgtgactc
     5821 agcacgt
//
LOCUS       DASSFQ010000011         6013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_157717, whole genome shotgun sequence.
ACCESSION   DASSFQ010000011 DASSFQ010000000
VERSION     DASSFQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157717"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..2485)
                     /locus_tag="VFH96_00390"
     CDS             complement(53..2485)
                     /locus_tag="VFH96_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972443.1"
                     /translation="METLFKDIRYGIRGLLKRPGFTVIALITLALGIGANTAIFSVVN
                     AVLLRPLPFQKPEELVIVWEDATFVGFPHNTPAPANYVDWKAQNQSFVDMAASHEDSF
                     NLTGDGEPERVSAYAVNANFFPLLGVQPLLGRGFLNEEDRPGANKVVVLSYSLWQSRY
                     GGDRNVLNRAILLNGEKHIVVGVMPASFQFFAKDVRLWVPIALSQEDWANRGGHYLAV
                     VARLKPGVSVAQAQADMNAVMHRISVDHPEETMEGKMGVVVVPVRDEFVGDARGSLIV
                     LLVAVAFVLLIACANVAGLLLARAVARRKEIALRLALGAGRPRVIQQLLTESLLLAVA
                     AGVLGSLLAYASFTFLQGLVPEQMALATSLKLDVRILVFTIAVSIVTGIIFGLVPALQ
                     SANFDLNDALKQSSGRVTSTGRLRSAMIVFEVALSLVLLVGAGLLIQTLFQLFRQYSM
                     LEPEKILTMRTILPLEKYKEPQQRDNYYQQVLQRVEHLPGVVAAGYSTSIPLAWKGGT
                     SGFYPEGLKAPLPGMAYDANHREVSTNYLKTMNIPLRHGRYFESSDNAQSVPVAIINE
                     TMARQYWPGENALGRRFKVGDPDEPGKQWKQIVGIVADIRQMGLDEPAKAEMYLPYQQ
                     ITDWPGYIPRDLAIRTTGDTSNLAGAVRQIIREIDPDQPVSNVATMAEVLGTEAGQRR
                     MGMIMLVGFASLALLLASLGIYGVLAYFVTQHTNEIGVRQALGATPRNILLLVLKKGM
                     GLTLVGVAIGLVASFALTRLMSSLLFGVTASDPLTFATVPLVLVMVALLACYIPARRA
                     TKVDPLVALRYE"
     gene            complement(2542..4947)
                     /locus_tag="VFH96_00395"
     CDS             complement(2542..4947)
                     /locus_tag="VFH96_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972444.1"
                     /translation="METLIKDLRFAVRSFLKRPGFLVIAVATLALGIGATTAMFTVVN
                     SVLLRPLQFPEPERIVLFYGINPRQGITQSNMSMPDIVDWQKQSQSFEQIAGFVSGGL
                     FLTAGDETERVRGTGVSGEFFPLFKTNPIQGRTLQAGDMQQGGEPVVVISYALWQRRF
                     GGATSVVNSKVMLNGKAATIVGVMPAGFNYPADSEAWTAFGLNEPDQPRDNRFISVVT
                     RLKPGVSMTQAQAEMDTINQRLAQNYGETNNGWTVRLLELRESLVGELRTSLLILLGA
                     VAFVLLIACANVANLMLARAVYRQKEIALRTALGASRLRIVRQLLTESMLLSVVSGVI
                     GFTLSLGLVRLLIAINPPNSPRVEEIGIDWRVFSVTLAVAVLAGLLFGLFPALQTSRP
                     NLNDTLKDSGQRGSQFGGRNRAGSLFIVSEIALSFILLAGAGLLIKSFLHLRQIDPGF
                     NADNVLAMRLSLPPGKYKQGEPRAQIYKQLIDHVKATPGVKSAGAVLSLPLGGDTFGV
                     WRGVVPEGRPQTLEEQTNAQNLPVTPDYFSTLQIPLQAGRLFTDQDDLNSTKVVIINE
                     TLARRLWPGENPIGRRFAVWRDEKFAREVVGVVGDTKETLDEDAGNQMYIPYAQDPTW
                     GSMSLVVRTNGEPAALAGAVREAIRSIDKAVPNYNLKTMNDVVSASAAPRRVPMLLLS
                     AFAGVAMLLAMLGIYGVTSYYVTQRTHEIGVRMALGAQIVDVLKLVLSRAMTLALIGI
                     GIGVVGAIALTRYLTTLLFGVKPIDVVTFVVVALILTLVVLVACLVPARRATKIDPLE
                     ALR"
     gene            complement(4990..>6013)
                     /locus_tag="VFH96_00400"
     CDS             complement(4990..>6013)
                     /locus_tag="VFH96_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6972445.1"
                     /translation="GYDEARGQQFYKQLQERLQALPGVESAAVMSYIPLSLNYNSNTI
                     FVEGKPAERGDNAPMAMTASVGPAYFKTMGTPLVQGREFTEADQAKTEQVAVVNEYFV
                     RRLMRELKTPAEAVGKRFSFEGAKGPWVEIVGVAKAGKYFNIAEEPRPFVWTPMTQNY
                     NSSGILVARTKGSPEGLFAAVRGQVQSLDANLPLFDVKTLTEHMRLALFPAKVAATVL
                     GVFGFVTLLLAAIGVYGITSYAVAQRTHEIGVRLALGAQLSDVLKLVLSHGLKLTIIG
                     AALGLFGAFLATRAITSVLYGVSATDPLTFTFVSLLLIGVALVASYVPARRATKVEPL
                     IALRNE"
BASE COUNT         1365 a         1937 c         1531 g         1180 t
ORIGIN      
        1 ccgttcgtat ttccggagac gggtggccac ggagggccac ccctacagtt ggctattcgt
       61 atctcaaagc aactaaagga tcgactttcg tcgcgcgccg tgccggtatg taacacgcca
      121 gcaacgccac catcacgagc acgagcggca cggtggcaaa cgtcaacgga tctgacgccg
      181 tcactccaaa caacagcgag ctcatcaaac gcgtaagcgc gaacgacgca accaacccga
      241 tcgccacacc aaccagcgtc agccccattc ccttcttcaa cacgagcaac agaatattac
      301 gcggcgtggc gccgagcgcc tgccgcacgc cgatctcatt cgtgtgttgc gtgacgaagt
      361 aagcgagcac gccgtaaatg ccgagcgacg cgagtaataa cgcgagcgac gcaaagccta
      421 cgagcatgat cattcccatg cgacgctgac cggcttccgt gccgagcact tccgccatcg
      481 tggccacatt cgaaaccggc tgatccggat ctatttcgcg aatgatctgt cgcactgcac
      541 cggcgagatt cgacgtatct ccagtcgttc gaatcgcaag gtcacgtggt atgtatccgg
      601 gccagtccgt gatctgctgg taaggcaaat acatctcagc tttagcaggc tcgtcgagtc
      661 ccatctgacg tatatccgcg acaatgccga cgatctgttt ccactgtttc cccggttcgt
      721 cagggtcgcc aaccttaaaa cgacggccca gcgcgttctc tcccggccaa tattggcgcg
      781 ccatcgtttc gttgatgatc gcgaccggaa cagactgcgc attgtcacta ctctcgaagt
      841 aacgcccatg tcgcagtgga atgttcatcg tcttgagata gttggtacta acctctcggt
      901 gattggcgtc gtaagccatg cccggaagcg gggctttgag cccttccgga taaaagccac
      961 tcgtgccgcc cttccacgca agcgggatag acgtgctgta accagccgcc actacgcccg
     1021 gcaggtgctc gacgcgttgc agcacttgct ggtagtagtt gtcgcgctgc tgcggctctt
     1081 tgtatttctc gagcggcagt atcgtgcgca tcgtgagtat cttttccggc tcgagcatcg
     1141 agtattgccg gaaaagttga aacaacgttt gaatgagcag acctgctccc acgagcaaca
     1201 cgagcgacag cgcgacttca aacacgatca tcgcgctacg caagcgaccc gtggaagtca
     1261 cacgcccact gctctgcttc aaagcatcgt tcaaatcgaa gttggcagac tgaagcgcgg
     1321 gcacgaggcc gaagatgatt cccgtgacga tcgaaaccgc gatcgtgaag acgagtatgc
     1381 gtacgtcgag tttcaaactc gtcgccaacg ccatctgttc gggaacaagt ccctgcagga
     1441 acgtgaaact cgcgtaagcc agcaacgatc cgagcacgcc agccgcaact gcgagcagca
     1501 aactctccgt caacaattgc tgaatcacgc gcggacgccc ggcgcccaac gccagacgca
     1561 acgcgatttc tttgcgacgc gcgaccgcac gcgccaacaa cagcccggcg acattcgcac
     1621 acgcaatcag cagtacgaac gcaaccgcga ccagcaacac gatcaacgag ccacgcgcat
     1681 cgcccacaaa ctcatcgcgc accggcacca cgacgacgcc catcttccct tccatcgttt
     1741 cctccggatg gtccacggaa atccggtgca tgacggcgtt catgtcagcc tgtgcctgcg
     1801 ctaccgaaac gccgggcttt aaacgcgcca ccacggccag gtaatgacca ccacgattgg
     1861 cccagtcctc ctgactgaga gcgatcggta cccacaggcg cacgtccttc gcgaaaaatt
     1921 gaaaactcgc cggcatcaca ccaaccacta tgtgcttctc tccgttgagc agaatcgcgc
     1981 gattgagcac attgcgatcg ccaccgtagc gactctgcca aaggctgtag ctgagcacga
     2041 cgactttgtt cgcgcccggc cggtcttctt cattcagaaa accgcgaccg agaagtggct
     2101 gcacgccgag cagcgggaag aaatttgcat tgactgcata cgcggaaacc cgctcaggct
     2161 caccatcacc ggtgagattg aaactgtctt cgtgactcgc agccatgtct acaaacgact
     2221 ggttctgagc cttccagtcg acgtagtttg ccggagcggg cgtgttgtgc gggaagccca
     2281 caaacgtcgc atcttcccaa acgatcacga gctcttcagg tttttgaaac ggcaacggcc
     2341 gcagcaacac cgcattgacg acactgaaga tcgccgtatt cgcgccaatg ccgagcgcga
     2401 gcgtgatgag agcaataacc gtaaaccccg gcctctttaa caggccacga atcccgtagc
     2461 gaatatcttt aaataaggtt tccattgcct attaacaccg ccgccacagc aaatctggct
     2521 ttaaatccgt gtcatctgtg gctaccgcaa cgcttccaat gggtcgatct ttgtagctct
     2581 tcgcgccgga accaaacatg caaccaacac gaccaaagtc aggatcagcg ccaccacaac
     2641 aaacgtcacc acatcgatcg gcttcacgcc aaacaacaac gtcgtcagat accgcgtcaa
     2701 cgcaatcgca ccaacaacac cgatcccgat tccgatcaac gcgagtgtca tcgcacgact
     2761 caagacgagt ttcagcacgt cgacgatctg tgcgccgagc gccatgcgca caccgatctc
     2821 gtgcgttcgt tgcgtcacgt agtaagaagt gacaccgtaa atcccgagca tcgccagcaa
     2881 catcgccacc ccggcaaaag cactcagcaa caacatcggt actcttcgcg gcgccgcaga
     2941 tgctgagacc acatcgttca tcgtcttcaa gttgtaattc ggcactgcct tgtcgatcga
     3001 acgaattgct tcgcgcactg cacccgcgag cgcagccggc tccccgttag tgcgcactac
     3061 gagcgacatg ctgccccacg tcggatcctg cgcgtagggt atgtacatct ggttgccggc
     3121 atcttcatcg agtgtctcct tcgtatcgcc aaccacgccg accacttcgc gcgcaaactt
     3181 ctcatcgcgc cagaccgcga agcgacgacc aatcgggttc tcgccgggcc acaagcgtcg
     3241 cgcgagcgtc tcgttgatga tcacgacttt cgtgctattt aaatcatctt gatcggtaaa
     3301 caaccgcccg gcctgcaacg gaatttgcag tgtcgagaag taatcaggtg tgaccggaag
     3361 gttttgtgcg ttcgtctgct cttcaagcgt ctgcggacga ccttcgggaa ccacaccgcg
     3421 ccacactcca aacgtatctc cgcccaacgg caaactgagc acagcgcccg cagacttcac
     3481 accgggcgtt gctttcacgt ggtcgatgag ctgcttgtag atctgcgcgc gcggttcgcc
     3541 ttgtttgtac ttgcccggcg gcagactcag acgcatcgcc agaacgttgt cggcattgaa
     3601 cccgggatcg atctgtctca ggtgcaggaa gctcttgatc agcagtcccg cgcccgcgag
     3661 caggatgaac gagagcgcga tctcagaaac gatgaacagg ctgcccgcac gattgcgtcc
     3721 accgaactgc gatccacgct ggccgctgtc tttgagtgtg tcgttgagat taggccgcga
     3781 cgtttgcagc gcgggaaaca gtccaaacag cagaccggca agcacggcca ccgcgagcgt
     3841 cacactaaac acccgccagt cgattccgat ctcttccacg cgcggcgaat tcggcggatt
     3901 gattgcgatc aacaatctca ccaacccgag actgagcgtg aaacctatca ccccactcac
     3961 gactgaaagc agcatgctct cagtcaggag ctggcgcacg atccgcaggc gactcgctcc
     4021 cagcgctgtt cgcagtgcga tctctttttg acgatagacc gcgcgcgcca acatgagatt
     4081 cgcgacgttc gcacacgcga tcagcaacac gaacgcaacg gcgcccaaca agatcagcaa
     4141 cgacgtcctt agctctccca ccaggctctc gcgcagctcg agcagccgca cggtccagcc
     4201 attgtttgtc tcgccgtagt tctgcgcgag ccgttggttg atcgtgtcca tttccgcttg
     4261 cgcctgcgtc atcgagacgc ccggtttcag gcgtgtcaca accgagatga agcggttatc
     4321 acgcggctgg tccggttcat tcaaaccgaa cgcagtccag gcttcggaat cggccggata
     4381 attaaatccc gcgggcatga cgccgacgat cgtggcggct tttccgttga gcattacctt
     4441 gctgttcacc acactcgtcg cgccgccgaa gcgtcgttgc caaagcgcgt aactgatcac
     4501 gactacgggc tcgccgcctt gctgcatgtc gccggcttgc agtgtgcgac cttgaatcgg
     4561 attggttttg aagagtggaa agaactcgcc ggacacacct gtgccgcgca cgcgctcagt
     4621 ctcgtcgccg gcagtcaaaa acagtccacc tgaaacaaac ccggcgatct gctcgaacga
     4681 ctggctctgt ttttgccagt cgacaatgtc cggcattgac atgttgcttt gcgtgattcc
     4741 ctgtcgcgga ttgatcccgt aaaacagaac gattcgctcc ggctccggga actgcaacgg
     4801 acgcagcagc actgaattca cgaccgtgaa catcgccgtc gtcgcaccaa taccaagcgc
     4861 cagcgtcgcc accgcaatca ccaaaaaccc ggggcgtttc agaaaactcc gcaccgcaaa
     4921 gcgtagatct ttaataagtg tctccatccc tataccccga tccgcgtttt caatccgcgt
     4981 taatccgcgt tactcgtttc tcaatgcaat taacggctca acttttgtgg cacgtcgagc
     5041 cggaacgtac gacgccacca gcgcgacgcc gatcaacagc aacgacacga atgtgaacgt
     5101 cagcggatcc gtcgcactca caccgtaaag cacactcgtg atcgcgcggg tcgcgagaaa
     5161 cgcaccaaac agtccgagcg cggcgccgat gatcgtcaac ttcaatccat ggctgagcac
     5221 gagcttcaac acatcgctga gctgtgcacc gagcgcgagt cgcactccga tctcgtgcgt
     5281 ccgttgcgcg actgcatacg acgtgatccc gtaaacaccg atcgccgcca gcagcagcgt
     5341 cacaaaccca aacacgccca gcacagtcgc agccaccttc gccggaaaca gcgcgagcct
     5401 catgtgctca gtcaacgtct tgacgtcgaa gagcggcagg tttgcatcga gcgattgcac
     5461 ctgcccgcgc accgcggcaa acaatccttc cggactgcct tttgtgcgag caacgagaat
     5521 cccactcgag ttgtagttct gagtcatggg cgtccaaaca aacggccgcg gctcttcagc
     5581 gatgttgaaa tacttgcccg ccttcgcgac gccgacgatt tcaacccatg gtccttttgc
     5641 accctcaaaa ctaaatcgtt tcccgaccgc ttcagcaggc gtcttcagct cacgcatcaa
     5701 acggcggaca aagtattcat tgacaaccgc gacttgctcg gtcttcgcct ggtctgcttc
     5761 agtaaattcg cgaccctgga cgagcggcgt gcccatcgtt ttgaagtagg ctggtccaac
     5821 actcgcggtc atcgccatcg gtgcattatc gccacgctcg gcgggcttac cttcgacgaa
     5881 gatggtgttg ctgttgtagt tgagactaag cgggatataa ctcatcaccg ccgccgactc
     5941 gactcccggt agcgcttgca agcgttcctg taactgcttg taaaactgct gcccgcgcgc
     6001 ttcgtcatag ccc
//
LOCUS       DASSFQ010000012         6913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1495690, whole genome shotgun sequence.
ACCESSION   DASSFQ010000012 DASSFQ010000000
VERSION     DASSFQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495690"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFH96_00405"
     CDS             complement(<1..519)
                     /locus_tag="VFH96_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HET6972446.1"
                     /translation="MTHNIHPSPIRYQGVMVSSTFEDLSEHRSALIKAIKGQGLTDVA
                     MENDSAKARVDLIDSSLQMVRDSAAYVGIISHKYGQIPKCPKRNPKNLSTTELEFDEA
                     QRLNRPTLLFIMGDKHQVMPGNVETNASKRKKLSGFRERAKKMTHDSDVHRVYATFES
                     LEEFKSQAIHAVA"
     gene            865..2448
                     /locus_tag="VFH96_00410"
     CDS             865..2448
                     /locus_tag="VFH96_00410"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HET6972447.1"
                     /translation="MPTRKAARRLWRKSKARLNLKRIYYSVELLLFIAIYFIVFSGNR
                     LRTLDTFGRRTDAIVSLLLVGAFILIHVIARRRLLPKIERFYAPAPYDERKIFFDLGQ
                     GSQNVTSIDQLYQRVAERVRNALDASNAAIFVRDDASGNFNLRILSAQGRTAQTDVDG
                     KRCQLGKRAFVVRRLMNLSTPLLIEPKEIETWSQALNSVLPETRAERAGEHEVLLAIK
                     STMLVQIRQKNELVGILSFGPRSRGFQYSVADRELLMSIAAQLALIIDNARLTERMVA
                     QERMRRELALAAEVQQRLLPSSAPKGVGMEVAGFCEPARGVGGDYYDFINFDNSQLGL
                     AIADVAGKGMPAALLMSTVQATLRSLTARNGSSSSASELSSIVGKLNRLLFNTTNGEH
                     YVTFFYATFDQSNQCLTYVNAGHNPPLYLQADEDFEVRQLTSGGLVAGAFEHAVYEQE
                     TVQMKSNDLLFLYTDGLSEALNTEGEEFGASRIMDTLKSIASLSADQIRDVVARRVKE
                     WCSGMSLYDDLTFVIMKVK"
     gene            2524..4899
                     /locus_tag="VFH96_00415"
     CDS             2524..4899
                     /locus_tag="VFH96_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972448.1"
                     /translation="MNFIQDLRYAIRMMAKRPGFTIIAALTLALGIGANTAIFSAVNA
                     VLLRPMPFPESEQLVDLAETFKPDGFGSVSVPYFEDWKNQSTVFAGMSAYSFTSFNLE
                     GGNTPQRIPGIRVSANYFDVLGVKPTLGRAFLPGEDVAGHQQVVVIGDDLWRRNFAAD
                     PNIVNQTIAVNGQKYTVVGVMPRELSSLYRTVQMWSPLVFPEQDRANRGDHKYLVIGR
                     LKPGVTLAQASEQMNGIAQRLEEQYKNGRGVRLRQIEELWTANVRSPLWMMMVAVGFV
                     LLIACTNVANLLLARATVRRREIGIRIALGAGRGRLIQQFLTEGLLLSVIGGALGIGL
                     AWWTMDVLGKLAFPFLPRSQEIGIDTRVLLFTLGVSMLTSVVFGLIPSLQAGKTDVQE
                     TLKEGGNTMSIGAVGAWLRPVLVVVEIAAAVVLLIGAGLMIRSVMRIREVQPGLQPQN
                     LLTAKISLPRDKYKDIDSTNHFYTTVLERLNTLPGVESAAVISHLPIEEMGKGKTYPP
                     NESPLVEFRVVSDNYFDTANIPLLRGRLFSKEAGDDKQSVVVINEAMAKQIWPNEDPV
                     GKRVGDDTQVTVIGVVGNVKNYGLLQPAAPEMYALYTLKTFWAEMRWNMRLMVRSSLD
                     DSNIAAAVRREVQAVDPGQPIYAVSTMNLVIENTVKDKSVNTTLLSVFAGVSLLLAVI
                     GVYGVMSYTVAQHTREIGIRMALGAQPRSILQLIVGRGLVLVGVGAVVGVFGSFGLTR
                     FIENMLFGVTPTDPLTFVMIVALLGFVALLACLIPAGRAMRVDPIVVLRHQ"
     gene            5033..5902
                     /locus_tag="VFH96_00420"
     CDS             5033..5902
                     /locus_tag="VFH96_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET6972449.1"
                     /translation="MSTDYSSTTTISDHDLTLAIPGDVGSVRARLVNAIQTLGYKVLG
                     DQPLYAKRGSQCSAKWDCSLNVLDYTTTLTISLKQTNDTAVLATFNYEVKSYMRMTKG
                     DRQTLAREAEAIAALAMERAAASACRTCGTQVTDESNFCRRCGAPLALDVPELEVLRL
                     TRNARSSYHNMFVGFIALLLAALTVVPWFVVNGARIVAPMLWVGIPLASYGLFLLLNG
                     ARQLHRTLNPATTKTIATAAHPAFKSAVTTTALPPAPVNSVTEVTTNLLSTKEPVPRK
                     NRNTSGLDSDQLM"
     gene            5943..>6913
                     /locus_tag="VFH96_00425"
     CDS             5943..>6913
                     /locus_tag="VFH96_00425"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6972450.1"
                     /translation="MRKTAAFLGVGALQNLVSDSMAIDMGSASTIIAVRGRGVVVDEP
                     SLVAIDTVTGDVIAIGREAQQKYGREERGVSVIAPMLNGVVADFERTREMLAHFVGEA
                     RGGLSHFSRRALMSVFADVTPVERRALLGAAQHAHVGRVGMIEEGLAAAFGAGLSVDD
                     VHASAVVDIGGGTTNVAIVANGKTIRARAERIGSSNIDAAISNHVRRHRGLLIGAKTA
                     ERLKLELASATVPTNLAQEIPVNGRDVLTRMPRSIEISAGEIYPVAQEVVRQIVAGVG
                     ATLGDLPPEVAGDIYDRGIILTGGGALFGGLDQYLRDETKLPVRVAD"
BASE COUNT         1462 a         1878 c         2033 g         1540 t
ORIGIN      
        1 tgcgacagcg tgaatagcct gcgacttgaa ctcttcgagg ctttcgaaag ttgcgtagac
       61 gcgatgcacg tccgagtcat gcgtcatctt ctttgcacgc tcacggaagc cactcagttt
      121 ctttcgcttt gacgcatttg tttcgacatt ccccggcatc acctgatgct tatcgcccat
      181 gatgaaaaga agagtgggtc gattcagtct ctgagcttca tcaaactcga gctcggttgt
      241 cgagagattc ttaggatttc gctttgggca ctttgggatc tggccatact tgtggctgat
      301 gattccgaca tacgcagcgc tgtcacgaac catctgtaac gaagaatcga tgagatccac
      361 ccgagccttt gccgagtcgt tttccatggc gacatcggta agaccctggc ctttgatggc
      421 cttgatcaat gcagaccggt gctcacttaa atcttcgaag gtgctggaga ccatcacgcc
      481 ctgatagcga atcgggctgg ggtgaatgtt atgcgtcatg gcgttgatcc ggctttagag
      541 taggaggaag cccttaatgg cgcttggcct caacgcttgt gagatcacta agaagtctgg
      601 cggataaagc ggcttaaaat ggtggaaaag ccgttatccg cggatgtact tttacataag
      661 ttgagttgaa atctcaaacc tcacatcggt ggtttgatgt ccgataagct ttagcttgtc
      721 ggaatgtcgc gacaaactaa aaagtttatc ggacacttgc agcgaatacc ttgccagata
      781 tctgcgcctg gtggtgtctc tcatattgaa gaagccaatt ctattcggag tctgaatctg
      841 atgcggatct tgccgcgcct caatatgcca acgcgtaaag cggcgcgccg cctgtggcgg
      901 aaatcgaagg cacgattgaa tctgaaacgg atttattact ccgtcgagct gcttctcttc
      961 atcgcgatct acttcattgt tttctccggt aaccgtctca gaaccctcga cactttcggg
     1021 cgtcgcactg atgcaatagt ctccctcctt ctcgtcggcg cgtttattct gattcacgtc
     1081 atcgcgcggc gccgtctgtt gccgaagatc gagcgcttct acgctcccgc gccgtacgac
     1141 gagcgcaaga tcttcttcga tctcggacaa ggctcacaaa acgttaccag catcgatcaa
     1201 ctctaccaac gggttgccga gcgagtcaga aacgcactcg acgccagcaa cgccgcgatc
     1261 ttcgtgcgcg acgacgcgag cggaaacttc aacctgcgca tactttccgc ccagggacgg
     1321 acggcgcaaa cggatgtcga cggaaaacgc tgtcagcttg gtaagcgtgc gttcgtcgtg
     1381 cgacggctga tgaatctttc aacgccgctg ctgatcgagc caaaagagat cgagacgtgg
     1441 tcgcaagcgc tcaactcagt gttgccggaa acacgagcgg agcgtgcggg cgagcacgaa
     1501 gtcctgctgg cgatcaagtc gacaatgctg gtccaaatca ggcagaagaa cgagctcgtc
     1561 gggatactct cgtttggacc acggagccgt ggctttcaat actcagttgc cgatcgcgag
     1621 ctgctgatgt cgatcgctgc acagctcgcg ctcatcatcg acaacgcgcg cctcacggaa
     1681 cgcatggtcg cgcaggaacg gatgcgacgt gagctcgcgc tcgcagccga agtgcaacag
     1741 cgactgcttc catcaagcgc tccgaagggt gtcgggatgg aggtggccgg gttttgcgag
     1801 ccggcgcgcg gcgttggcgg tgattactac gacttcatca acttcgacaa cagccagctc
     1861 ggtctcgcga tcgcagatgt ggcgggtaaa gggatgccgg ccgcgttgtt gatgtcgaca
     1921 gtgcaggcca ccttgcgaag tttgacggcg cgcaatggct cgagtagttc tgccagcgag
     1981 ttgtcgtcga tcgtgggtaa actcaaccga ctgctcttca acaccacgaa cggcgaacac
     2041 tacgtcacgt tcttctatgc aacgttcgat cagtctaacc aatgtctgac ctacgtgaat
     2101 gcaggacaca acccgccgct atacctgcag gctgatgagg attttgaagt tcgccagctc
     2161 acttcaggcg gactggtcgc gggcgcgttt gagcacgctg tttacgaaca agagactgtg
     2221 cagatgaagt cgaatgatct gctgtttctt tacacggacg gtctcagcga agcgttgaac
     2281 acggaaggcg aagagtttgg ggcgagcagg atcatggaca cattgaagtc gatcgcttcg
     2341 ctctccgcag atcagattcg cgacgtcgtg gcgcggcgcg tgaaggaatg gtgctcgggc
     2401 atgtcgctct acgacgatct cacttttgta atcatgaaag tcaaataggc cacaaaaggc
     2461 acaaaaagca caaaagatat tttttgtgct ttttgtgcct tttgtggcta aaaaggagaa
     2521 gcgatgaact tcatccagga tctgcgctac gcgattcgaa tgatggcgaa gcggccgggc
     2581 tttacgatca tcgccgcgct cacgctcgcg ctcggcatcg gcgccaacac cgcgatcttc
     2641 tccgccgtca atgccgttct tctcaggccg atgccgtttc ccgagtcgga acaactcgtc
     2701 gatcttgccg agacgttcaa accggatggt ttcggttccg tctccgtgcc atatttcgaa
     2761 gactggaaaa accagagcac agtcttcgcc ggcatgtctg cctactcgtt caccagtttc
     2821 aatctcgaag gcggcaacac tccacagcgc attcccggca ttcgcgtcag cgccaactac
     2881 ttcgacgtgc tcggagtgaa acccactctg ggccgcgcgt ttcttcccgg tgaagacgtg
     2941 gccggtcatc agcaagtggt cgtgatcggc gacgatctct ggcgtcgtaa ctttgccgcc
     3001 gatccgaata tcgtcaatca aaccatcgcg gtgaacggac agaagtacac cgtcgtcggc
     3061 gtgatgccgc gcgagctttc gtcgctgtat cgcacggtgc agatgtggtc gccgcttgtc
     3121 tttcccgagc aggaccgcgc gaatcgaggc gatcacaagt acctcgtgat cggacgtcta
     3181 aagccgggcg taacgctcgc gcaggcaagc gagcagatga acggcatcgc gcagcgactc
     3241 gaagagcaat acaaaaacgg ccgcggcgtc aggctgaggc agatcgaaga actgtggacc
     3301 gcgaatgttc gatccccact ctggatgatg atggttgccg tcggattcgt gctgctgatc
     3361 gcgtgtacga acgttgcgaa cctgctactc gcgcgcgcca cggtgcgtcg tcgcgagatc
     3421 ggcatccgta tcgcgctcgg cgccgggcgc gggagattga ttcaacagtt tcttaccgag
     3481 ggattgttgt tgtcggtaat cggcggcgcg ctgggaattg gactcgcgtg gtggaccatg
     3541 gacgttctcg gcaagcttgc ttttccgttt ctgccacgct cgcaggagat cgggatcgat
     3601 acgcgggtgt tgttgttcac gttaggtgtg tcgatgctga cgagcgtcgt gtttggtttg
     3661 attccgtcac tgcaagccgg aaaaaccgac gtgcaggaga cactgaaaga aggcggcaac
     3721 acgatgtcga tcggcgcggt cggtgcctgg ctgcgtcctg ttctggtagt ggttgaaatc
     3781 gcggcggcgg tagtcttact gatcggcgcc ggcctgatga ttcgcagcgt gatgcgcatt
     3841 cgcgaagtcc aacccggact acagccgcaa aacctgctca ctgccaaaat ctctctgccg
     3901 cgcgacaaat acaaagacat cgattcaacg aatcattttt acaccacagt gctggaacgg
     3961 ctcaacactt taccgggcgt cgagtcagcg gcagtgatct cgcatctgcc gatcgaagag
     4021 atggggaaag gtaaaacgta tccgccgaac gagtctccgc tcgttgagtt tcgcgtagtc
     4081 agtgacaact acttcgatac agcgaacatt cctttgctgc gtgggcggct ctttagcaaa
     4141 gaggcgggcg acgataagca gtctgtcgtc gtgatcaacg aagcgatggc gaaacagatc
     4201 tggcccaacg aagatccggt tggcaagcgc gtcggagatg atacgcaagt aaccgttatc
     4261 ggagtcgtcg gcaatgtgaa gaattatggt ttattgcagc ccgcggcgcc ggaaatgtat
     4321 gcgctctaca cgctgaagac tttctgggcc gagatgcgtt ggaacatgcg actgatggtg
     4381 cgatcgagtc ttgacgacag caacatcgct gcggctgtgc gacgcgaagt gcaggcagtc
     4441 gatcccgggc agccgattta tgcagtgagc acgatgaatc tcgtgatcga gaatacggtg
     4501 aaggacaaga gcgtgaacac gacgttgttg agtgtgtttg ccggcgtgtc attgttgctc
     4561 gcagtgatcg gtgtttacgg tgtgatgagt tacacggtgg cgcagcacac gcgcgagatc
     4621 ggaattcgga tggcgctcgg tgcgcagccg cgatcgattc tgcagctcat cgtgggtcgc
     4681 gggctcgtgc tggtgggcgt cggcgcggtg gttggcgtgt ttggctcgtt cggtctgacg
     4741 cgcttcattg aaaatatgct cttcggcgtt actccgaccg atccgctcac attcgtgatg
     4801 atcgtagcgc tgctcggatt cgtcgcgctg ctggcgtgcc tgattcccgc gggccgtgcc
     4861 atgcgcgtcg atcccatcgt cgtgctacgc caccaataag actttttagc cacaaaaggc
     4921 acaaaaagca caaaaagaga tattgaagtg agcggttatc tcttttgtgc cttttgtgcc
     4981 ttttgtggct tcttattaga aacccttttg cggctcgaat cgtacacgcc ctatgagcac
     5041 agattactca tccaccacca cgatcagcga tcacgattta accctggcaa tcccgggcga
     5101 tgtcggtagc gtacgggcgc gtctcgtcaa tgctattcag acgcttggtt acaaagtgct
     5161 cggcgaccag cctctctatg caaagcgggg cagtcagtgc tccgcaaagt gggactgttc
     5221 tcttaatgtt ctagactaca ccaccacgct cacaatctcc ttgaaacaga caaacgacac
     5281 cgcggtgctc gctacgttca actacgaagt gaagagctac atgcgcatga ccaagggcga
     5341 tcgccaaacg ctcgcgcgcg aagcggaagc gatagccgcg ttggcgatgg aacgcgcggc
     5401 ggcctcggcg tgtcgcactt gcggcaccca ggtgactgat gagtcgaact tttgtcgccg
     5461 ctgcggagcg ccgcttgcgc tcgatgttcc ggagctcgaa gtgctgcgtc tcacaagaaa
     5521 cgcgcgcagt agttatcaca acatgttcgt cggcttcatc gcactattac ttgctgcgct
     5581 gactgtggtg ccgtggttcg tcgttaacgg cgcaaggatt gtcgcaccta tgttatgggt
     5641 tggcattccc ctcgcgagtt acggattgtt tttattactc aatggggcgc ggcaactgca
     5701 ccgcacgctc aacccggcca caaccaagac catcgcaacc gccgcacacc ctgcattcaa
     5761 atcagcggtc acgacaactg cgctgcctcc cgcgccggtg aattcggtga ctgaagtgac
     5821 cacaaacctc ctatcgacta aggagccggt acctcgcaaa aaccgcaata cctcaggcct
     5881 ggatagcgac caattgatgt aacaccgcgc gctatgcgtt atcatggcgc gcgatggcgt
     5941 tcatgcggaa gacagcggcg ttcctcggtg tcggcgcttt gcagaatctc gtgtccgact
     6001 cgatggcgat cgacatggga tcggcgtcga cgatcatcgc ggtgcgcggg cgcggcgtcg
     6061 tagttgatga gccttcgctc gttgccatcg atactgtgac cggcgatgtg atcgcgatcg
     6121 gacgcgaagc gcagcagaag tatggtcgcg aagaacgcgg cgttagtgtg atcgcgccga
     6181 tgctcaacgg cgtcgtggca gactttgaaa gaaccaggga gatgctggcc cattttgtcg
     6241 gcgaggcacg cggtggtctc tcgcatttta gccggcgcgc gttgatgagc gtgtttgctg
     6301 atgtcacgcc ggttgagcga cgcgcgttac tgggtgcggc gcaacacgcg cacgtcggtc
     6361 gcgtcggcat gatcgaagaa ggtctggccg cggcgtttgg agcaggcctt agcgtagacg
     6421 atgtgcatgc ttcggcagtg gtggacatcg gcggcggcac caccaacgtc gcgattgtcg
     6481 cgaacggtaa gacgattcgc gcccgcgccg agcggatcgg aagctcaaac atcgacgctg
     6541 cgatcagcaa tcacgtgcga cgtcatcgcg gtttgctgat cggtgcgaag acggcggaac
     6601 gactcaagct cgagctcgct tcagcaactg tgcccacgaa tcttgcgcag gagattccgg
     6661 tcaacggtcg tgacgtgctc acgaggatgc cacgctcgat cgagatcagt gcaggtgaga
     6721 tttatccggt ggcgcaggaa gttgtgcgcc agatcgtggc gggcgttggc gcaacgctgg
     6781 gagatctccc gcctgaagta gcgggcgata tttacgatcg gggaatcatt ctcaccggtg
     6841 gtggcgcgct gtttggtggt ttagaccagt acttacgtga cgagacgaaa ctgccggtgc
     6901 gcgtcgctga cga
//
LOCUS       DASSFQ010000013         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_902915, whole genome shotgun sequence.
ACCESSION   DASSFQ010000013 DASSFQ010000000
VERSION     DASSFQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902915"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFH96_00430"
     CDS             complement(<1..1166)
                     /locus_tag="VFH96_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6972451.1"
                     /translation="MITALYVLLVLVLVLANGFFVASEFALVGVRRSRIQMQAVTGDV
                     RAQRLVALLDNLNAYISATQLGITMASLALGWVGEPVFAHLLEEPLRGRVSEVTLHTI
                     SFAIAFSIITFLHIVLGELAPKTLALERAEGVALAISWPLQAFYKVFQWPIRLLDWSG
                     TRVVRLFGLHPSGEHGSVYTEGELRHLIDVSRQSGHLESEEQQLIHKVFDFSDAEVKE
                     AMVPRPQVVSLPVTATLDDVRRAFLTGGYSRLPVYRKDLDDIVGLLFRKDLDMGRVTN
                     EEDFDLRRWVRPPVFIPSSASLGIALKRMQASRVHFVFVVDEHGGLEGILTLEDLLEE
                     IVGEINDEYDEETREQIRKDGETYILDGMLPVRDANKRFGIGLTENEGYTTIAG"
     gene            complement(1166..2185)
                     /locus_tag="VFH96_00435"
     CDS             complement(1166..2185)
                     /locus_tag="VFH96_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET6972452.1"
                     /translation="MVELFPFSEYWWFYAAFVGLVLVFLTVDLGIFHRKAHAVGFREA
                     ATWSVVWITLSLIFNFLLYQYALWKFPQDPRLMAILGFDPNAEAWRVALEFLTGYVIE
                     KSLSVDNIFVFVMVFSYFAIPAKYQHRVLFYGILGALIFRAIFVALGAALMQYHWVVV
                     FFGVFLIFTGLKIFFAKEKGVNPEKNLLIRLLRRVIPVTPNLQGGKFFANIDGRHFAT
                     PLLVALVFLEATDIIFAVDSVPAIFAVTKEPLIVFTSNIFAILGLRALFFMLADAVDK
                     FYLLKYGLGVVLVFVGLKMVWLNQLFGGHFPIWVSLGIICGVIGLSILLSFIFPRTQA
                     AETSN"
     gene            2496..3293
                     /gene="ccsA"
                     /locus_tag="VFH96_00440"
     CDS             2496..3293
                     /gene="ccsA"
                     /locus_tag="VFH96_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET6972453.1"
                     /translation="MAVLAAYLIAAIHSILAFVNKRRSLQRVAEWSTIIGFVLHTGAL
                     MADWAIDGHYPLFGLREALSFLAWALVALYALARYRYRTQAVGAFTMPLISALTFVAL
                     LMGTNPSTTPASFSATWLFPIHTTLLIFAYAAFFIVFMASVMYLVQERALKLKTFGAS
                     FHRLPALTTVNALATSAAALGLTLLTLGIATGMFWSAARSGRFWNNDPKEIFALLTWL
                     LYFGLTVYRSTANWRGRSAAWLGVVGFGLVIVTFIGAPFLGGYHVFK"
     gene            3335..>4522
                     /gene="hemA"
                     /locus_tag="VFH96_00445"
     CDS             3335..>4522
                     /gene="hemA"
                     /locus_tag="VFH96_00445"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET6972454.1"
                     /translation="MSILLIGVNHKTAPVEIRERLALNDEACATGLRQLVDGDIVREG
                     LIVSTCNRVEILSATASDQLGFGAERITQFLDTSGHLPAGFLEAHLYHHTDEEAVRHL
                     FRVASSLDSMVVGEPQVLGQVRHAYALGVEAGTVGRVLNRLVHHTFKVAKRVRSETGI
                     AANAVSISYMAVELGKKIFDSLKGRTVLLVGAGEMAELSARHLVNAGASRVIIANRTE
                     ETARQMATEFGGFAVSLDQLDQVLHEADIVICSTGAPDYVVTAEQTRKALVRRRRNRP
                     TCFIDISVPRNIDPAVSNIPNVFLFDIDDLESVISSNIREREREAERAELIVQSEVMQ
                     FQQKLRLMDIGPSIGALRQNMQDVARAELAKQRKRLGPLTKDQEEAIESLLMSTVNKI
                     SHPI"
BASE COUNT         1054 a         1301 c         1209 g          958 t
ORIGIN      
        1 aacccggcga tcgtcgtgta accttcgttt tccgtcagcc ctatgccgaa acgcttgttg
       61 gcatcgcgca cgggtagcat tccgtcgagg atgtacgtct caccatcttt ccggatctgc
      121 tcgcgcgtct cttcgtcata ctcgtcatta atctcaccga cgatctcttc aagcagatct
      181 tcgagcgtga gtatgccttc gagtccacca tgttcgtcca cgacaaacac aaaatggacc
      241 cgcgacgcct gcattcgctt caacgcgata cccagcgacg cagacgatgg aataaacaca
      301 ggcggccgca cccaacgcct cagatcgaaa tcctcttcat tcgtgacccg ccccatgtcg
      361 aggtcttttc gaaagagcaa accaacaatg tcgtccagat cttttctata aaccggcaga
      421 cgcgaatagc cgccggtgag aaaagctcgt ctaacatcgt cgagagttgc agtaaccggc
      481 aaggacacga cttgcggacg tggcaccatc gcttctttaa cttcagcgtc agagaagtcg
      541 aagaccttat ggatcaactg ctgttcttcg gattcgagat gaccactctg tcgcgacaca
      601 tcgatcaagt gtcggagttc accttccgtg tagactgatc catgctcgcc gctcggatgc
      661 agtccaaaca gacgaaccac gcgcgtgccg gaccaatcaa gcaatcgtat gggccattga
      721 aacactttgt agaacgcctg cagcggccag gagatcgcga gcgcaacccc ttccgcgcgt
      781 tcgagcgcca gcgttttcgg cgcgagttct cccaacacaa tgtgcagaaa cgtgatgatt
      841 gaaaacgcga tcgcaaacga gatcgtgtgc agcgtcacct ccgagacacg tcctcgcagc
      901 ggttcctcga gcaaatgtgc aaacaccggc tcgccgaccc agccaagagc cagcgacgcc
      961 atcgtgatgc ccaactgcgt tgccgaaatg tatgcattca gattatcgag cagtgcgact
     1021 aaacgttgcg cacgcacatc gccagtcacc gcctgcatct gaattcgcga ccgccgcaca
     1081 ccaaccaagg cgaactccga ggcgacgaaa aaaccattcg ccaacacgag caccagcact
     1141 aacagaacgt aaagcgcggt aatcatcaat tactagtctc agctgcctga gttctcggaa
     1201 aaatgaacga cagcaaaatc gacaaaccaa tcactccgca gatgatcccc agcgaaaccc
     1261 agatcggaaa atgcccgcca aacagttgat tcaaccagac catcttcaag ccaacgaaca
     1321 cgagcacaac gccgagtcca tacttcagca ggtaaaactt gtcgaccgcg tctgcaagca
     1381 tgaagaagag cgcacgcaag ccaaggatcg cgaagatgtt tgacgtgaag acgataagcg
     1441 gctctttggt caccgcaaag atcgcgggca ctgaatcaac cgcgaagatg atgtcagtcg
     1501 cttcgagaaa aacgagcgcg acgagcagcg gtgtcgcaaa gtgtctgcca tcgatgttcg
     1561 caaagaactt tccgccctgc agatttggcg tgaccgggat cacgcgacgc aacaacctga
     1621 tcagcaagtt cttttccgga ttgacgccct tctccttcgc gaagaagatc ttcagaccgg
     1681 tgaagatcag aaacacgcca aagaacacga ccacccagtg atactgcatc agcgccgcac
     1741 ccagcgcgac gaagatcgcg cggaagatga gcgctccgag aatgccgtag aacaagacgc
     1801 gatgctggta cttcgctggt atcgcgaagt aagagaagac catcacgaag acgaagatgt
     1861 tgtcgaccga cagcgacttc tcgatcacgt agccggtgag aaactcgagc gcgacacgcc
     1921 acgcttcggc attcgggtca aaccccagta tcgccatgag acgcgggtcc tggggaaact
     1981 tccagagcgc gtactggtaa agaagaaagt tgaagatcag cgagagcgtg atccaaacga
     2041 cgctccacgt tgcggcttca cgaaagccga cggcgtgcgc tttgcggtga aagatgccga
     2101 gatcgacggt gaggaaaacg agaacgagcc ctacgaaagc cgcgtagaac caccagtatt
     2161 cagaaaacgg aaaaagctca accatacctc atcctttcgc attcgaaagg tcttggtcgt
     2221 tgagctttga gcttttccca gtaagccggg cgccgtttcg gcaccgtgtt gacgacttac
     2281 cggacccgtg atgggtgacc tactcccctt taaaacctca gtcactctac agcaaaacca
     2341 ggaacaaagc cacaaaaggc acaaaaggcg ctgtaagtgt ccgataaact ttagtttgtc
     2401 gcgacattcc gacaagctaa agcttatcgg acatctcttt tgtgcttttt ctgccttttg
     2461 tggctaaaat gcgttctata ttgagatcct tacttttggc agttttagcg gcgtatctga
     2521 ttgccgcaat ccattcgatt ctggcgttcg taaataaacg ccgctctctg caacgtgttg
     2581 cggagtggtc gacgattatc gggttcgtcc tgcacacggg cgctttgatg gctgactggg
     2641 ccatcgacgg tcattacccg ctgttcggct tgcgcgaggc gctttcattc ctggcctggg
     2701 ctctcgtcgc gctttacgcc ctcgcgcgct atcgctaccg gacccaggcg gtcggcgctt
     2761 ttacgatgcc gctgatctcg gcgctcacat ttgtcgcgtt actaatggga acaaacccaa
     2821 gcaccacgcc ggcttcgttt tcggcgacgt ggttgtttcc gatacatacg acgttgttga
     2881 tctttgcgta cgcggcattt ttcattgtct ttatggcgag cgtgatgtac ttagtccagg
     2941 agcgtgcact gaaactcaag acgtttggcg cgtcctttca ccggctgccg gcactgacca
     3001 cggtcaacgc cctcgcaacg agcgccgcgg ctctcggact gacgctgctg actctcggga
     3061 tcgcaacggg aatgttctgg tcggcggcgc gctcgggccg cttttggaac aacgatccga
     3121 aagagatctt tgccctgctc acgtggttac tttatttcgg tttgactgtc tatcgttcga
     3181 cggcgaactg gcgtggtcgc agcgccgcat ggctcggcgt ggtgggtttt ggcctcgtga
     3241 tcgtcacatt catcggggca ccattcttag gtggatatca cgtgtttaaa taaagctctt
     3301 ttatgcgttt ttgtgccttt ttgtggctaa acaaatgtcc attcttctca tcggagtcaa
     3361 tcacaagaca gctccggtag agatccgcga gcgactcgcg ctcaacgacg aggcctgcgc
     3421 cactggcctg cgccaacttg tcgacggcga catcgtgcgc gaaggattga tcgtctcgac
     3481 ctgcaatcgc gtcgagattc tcagcgcgac cgcgagcgat caactggggt tcggcgcgga
     3541 acgcatcacg cagtttctcg acacatccgg ccacttgccg gcgggttttc ttgaagcaca
     3601 tctctatcac cacactgacg aagaagccgt gcggcacttg tttcgcgtcg cgtcgtcgct
     3661 ggattcgatg gtcgtcggcg agccgcaggt gctgggccag gtgcgacacg catacgcgct
     3721 cggcgtcgaa gccggcacag tcggtcgcgt actgaaccgt ctggtccacc acacgttcaa
     3781 ggtcgcgaaa cgagtcagaa gtgaaaccgg catagccgcg aacgccgtgt cgatcagtta
     3841 catggccgtc gagctcggca agaagatctt cgactcgctg aaaggccgta ccgtgttgct
     3901 cgttggcgct ggcgagatgg cagagctttc ggcgcgacat ctggtgaatg ccggcgcctc
     3961 gcgtgtgatc atcgctaatc gcactgaaga gacggcgcgg cagatggcaa ctgagtttgg
     4021 cggcttcgcc gtttcactcg atcagttgga ccaggtgctg cacgaagctg acatcgtgat
     4081 ctgttctact ggcgcgccgg attacgtggt tactgcggaa cagacgcgca aagccctcgt
     4141 gcggcggcgg cgcaatcgtc ccacgtgctt tatcgatatc agcgtcccgc gaaacatcga
     4201 cccggcagtc agcaacattc ccaacgtctt cctcttcgat atcgacgacc tcgagtcggt
     4261 gatttcttca aacattcgcg agcgcgagcg tgaagcggag cgtgctgagc tgatcgtgca
     4321 gtcggaggtg atgcagtttc agcagaagct tcgcttgatg gatattggac caagcatcgg
     4381 cgcgctgcga cagaacatgc aggacgttgc tcgtgccgag ctggcgaagc aacgtaaacg
     4441 acttggcccg ctgacaaagg atcaggaaga ggcgattgag tcgttgctga tgtcgacggt
     4501 caacaagatt tcacatccaa tc
//
LOCUS       DASSFQ010000014        20037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1496655, whole genome shotgun sequence.
ACCESSION   DASSFQ010000014 DASSFQ010000000
VERSION     DASSFQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20037
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496655"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFH96_00450"
     CDS             complement(<1..721)
                     /locus_tag="VFH96_00450"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HET6972455.1"
                     /translation="MRKTVALILLILLTQPLVFAQKQRSLTEQQRIIHVLNRLGFGAR
                     PGDVERVKAMGLENYINQQLNPEKISDTVAENRLRELSTLNMTTAELYEKYPQPGQLL
                     RQLQAKGMVPEEMPADPAKNPLDNEKNRKVIDEYYRENGLQRPQRIIAELQASRILRA
                     VYSERQLQEVMVDFWTNHFNIFANKGADRWLLPAYDRDTIRPNALGKFSTLLQATAQS
                     PAMLFYLDNFQSVSPNANQQRR"
     gene            complement(813..2177)
                     /locus_tag="VFH96_00455"
     CDS             complement(813..2177)
                     /locus_tag="VFH96_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET6972456.1"
                     /translation="MIFLLAANLVVAAQTKQQSRRAAPVRNRAISNIVRDIDARNIEA
                     TIRKLVSFGTRNTLSEQNDPKRGIGAARDWLYAEFLKAAEASNGRMTVEKQSYEQAKA
                     ARVPEPTMLTNVVATLKGSQPEAADRIYVVSGHYDSMCNSPTDAKCDAPGANDDASGT
                     AAVLEMARVMAKYEFDATIVFMVVAGEEQGLLGSTHFAEQAKQKNWNVDAMFTNDIVG
                     NSLGGNGVRDRGHVRVFSEGVPSNETPAEATTRRSVGGENDSASRQLARFIKETGDAF
                     VPQFKVTMIYRRDRYGRGGDHIPFLERGYPAVRFTEVNEEFRHQHQNVRVENGVQFGD
                     LPEFVDFGYVVNVARVNAASLAALAWAPSRPAGVTIVAQRLSNDTELRWDANKEPDLA
                     GYEIVWRETTEAVWTDSKPVGNVTSFTMKGMSKDNYFFGVRSIDKDGNKSPVSYPRPQ
                     TRNQ"
     gene            complement(2238..2717)
                     /locus_tag="VFH96_00460"
     CDS             complement(2238..2717)
                     /locus_tag="VFH96_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6972457.1"
                     /translation="MYCPTCGNEITVELKYCNRCGANLTLPTTSAPMMLAPPKLTVPS
                     IILGITILGGMGVIFGSATEFARQNMNWAAIVWMVLFSSATLFGCVGLLIRFLTKMMT
                     LQREIAPPQQQLRPTFNDRPAMPHLPPRMEPPLGSVTENTTRTFSPIYRDSADRGTR"
     gene            complement(2821..3933)
                     /locus_tag="VFH96_00465"
     CDS             complement(2821..3933)
                     /locus_tag="VFH96_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6972458.1"
                     /translation="MKQETNNEMDLLLRRLGRHDDAAVPEDHLDADELSAYAENALPA
                     AARARYTGHLAECSRCRSLIAQLSSSAGVVATTDNAAVPEPSGFRKFLASLFTPMVLR
                     YAAPALGLIVVAAIGFVVLRRNQPEAYVARNTAADTHQQPVASPAASSGVVFSVSNPS
                     PSPGRVDEEKKEKTAPAQPAGPPNMPPSVTSVEAQVSQDKAVAQPKPEEQTASANAPP
                     PVKSEPTATPEQSPRAAETEAAKNEVKVQPVAGSQARKLRGAQRDDKDSLARAKKPAD
                     AASAPASGEGAQLQIGSVDDATSNTRTVAGRRFRQQSGVWVDTAYDSSKDAVTLSRGS
                     EQYRALVADEPAIKTIADQLEGEIIVVWKGHTYRIR"
     gene            complement(3985..4899)
                     /locus_tag="VFH96_00470"
     CDS             complement(3985..4899)
                     /locus_tag="VFH96_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6972459.1"
                     /translation="MNTGIDKILNDGAGRMLARASDNRSLSREAMVPRITGAVEKYLL
                     KDDPDTPHAEISKFIDELQADDLCLIIACERGDEKAWNDLVERFTVTVRSAARAASKN
                     EDAAEDLAQSIWAELYGLRTRKDGMPASKLAYYSGRGSLGGWLRAVVAQLAVDVFRKQ
                     SRLVQTEEDTDLDRLARVAEGQPIVAAIPSPEESISNRFAQTDMQQALNQCVQELPAE
                     DRLLVKLYYFDNLRLREAGAVLGVHEATASRRLTRIQTDLRKRVTKILVDERGWTQAE
                     TERSFAEVAQHLDMDLESILSKEAADLR"
     gene            4985..5629
                     /locus_tag="VFH96_00475"
     CDS             4985..5629
                     /locus_tag="VFH96_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6972460.1"
                     /translation="MLRVTEIFRSIQGESTHAGRPCTFVRLTGCPMRCVWCDSEYTFT
                     GGEHYSIDNIMDQVAAFGCRLVEVTGGEPLAQKEAFQLIQRLCDDGYEVLIETGGYVS
                     TANVDHRAKIILDIKCPASGEEPRNDWSNLDRLRADKDEVKFVIADEGDWIYAKNLIV
                     EQALESRTKAVLISPAWGQVDLQQLADWIASSGLNVRMQLQLHKYIWGPDVKGV"
     gene            5629..6378
                     /gene="queC"
                     /locus_tag="VFH96_00480"
     CDS             5629..6378
                     /gene="queC"
                     /locus_tag="VFH96_00480"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HET6972461.1"
                     /translation="MGGSQEIETEELETTIENDGPQLAICLVSGGMDSCVTAAIANEE
                     NDELAFLHVSYGQRTEKREREAFEALADHYNVKSRLVISLEHLAKIGGSSLTDARIPV
                     TTADLSVPGIPSSYVPFRNAHLLSAAVSWGEVINATSIYIGAVAEDSSGYPDCRPEFY
                     DAFQNVLDIGTKPETRITIETPVIAMRKSEIVRRGIELGAPLHLTWSCYKESESACGN
                     CDSCALRLRAFREAGVTDPIPYLLDSSRFAY"
     gene            6476..7816
                     /locus_tag="VFH96_00485"
     CDS             6476..7816
                     /locus_tag="VFH96_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET6972462.1"
                     /translation="MIGKHFFGALAVAILVASASLTAVAQVGELRGHIWMQQADGQKV
                     PLADAQIDVFRTDMSAKYNTKTNKKGEFVFAGLPFIGTYTVAASHPTAAPNFQPGVKA
                     GRDIDVELTLSPGNGKRLTFEEIKGAGGGSATPPSGGTPQPSAEDKAKLEELRKKNEA
                     IEASNKKITEANEVIAKTFKAGNEALQAAGAASKANQSEEAIAKYTAAVAQYDEGLAA
                     DADQPAILTNKAVALKGRGVEKFNTTVRSKTLDDAGRTAGLQAAKDDFKAAAETSAKA
                     VTMIKALPAPTEAADLQRFNGNKYAAMLTQAESMRLYVSKADGTQADAGLAAYKDYIA
                     IETDPAKKAKAQLDMAQMLLDAGASDKALTEFKAILAENPNSPEANLGAGLAVYATGD
                     KAKFQEAANFLQKFVEVAPDGNAMKDDAKAILTELKNSENVSPEKTSRPARKKP"
     gene            8082..9881
                     /gene="pilB"
                     /locus_tag="VFH96_00490"
     CDS             8082..9881
                     /gene="pilB"
                     /locus_tag="VFH96_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET6972463.1"
                     /translation="MSAKLGEILVRENLISPQHLREALDYQREHGGRLGFNLVKLGLI
                     SDDMITAVLSRQYGIPSVNLDLFNIDPSVISLIPQEVASKHSVLPLSRVGATLTLAMV
                     DPTNVFAMDDVKFMTGLNVEPVVVAEGSVQQAIAKYYGSSREIELATVTTEELPKNGS
                     KNNGANGGITKDDLVSLDSIDFDHNQSEDVEVLEDNEEIDLSTLSKMSEDAPVVRLTN
                     VLLVDSLRRGASDIHIEPYEKELRIRFRIDGVLYDVMHPPLKMRDALISRIKIMSKLD
                     ISEKRLPQDGRIKIKVKVDSRSRELDFRVSTLPTLFGEKVVLRLLDKQNLMLDMTKLG
                     FEPESLTKFKRNISKPYGMVLVTGPTGSGKTNTLYSALQSLNTVETNIMTAEDPVEFN
                     LAGINQVQMKEQIGLNFAAALRSFLRQDPNIVLVGEIRDFETAEIAIKAALTGHLVLS
                     TLHTNDAPSTISRLMNMGIEPFLVATSVNLIQAQRLIRRVCKDCKQETSIPAEAFIEV
                     GFTIEEAKTMKTFKGKGCATCNNTGYKGRIGLYEVMEITDELRELILIGASALELRKK
                     AIDDGMITLRESGLHKIRAGVTTIEEVVRETVA"
     gene            9897..10739
                     /locus_tag="VFH96_00495"
     CDS             9897..10739
                     /locus_tag="VFH96_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972464.1"
                     /translation="MLRPIAIALILVLSVGIMLPFANEAHGLRQTVQVGQRRHHRYRS
                     RAWWRRYHARLRQKRLAAEFAAHRNATLSLPQNLSVADLQGLNGPQLPSLPSNSAAPA
                     TLPPPEMTAAPVAAISNAAHASSLSNGETRPRVVNPNHPAFKASANATPLPGTMNLAV
                     IAMSRPNPAFLTSREEKKMLGGVAVADMRRMVIDKMVSSGGWVVNDFVREVNGSRVFV
                     VTARTPKDALTPEKAWTFYFTEAGGRIYGLTTDAPVEFAERMTNEAERFIESLRAKAE
                     AGTR"
     gene            10842..12020
                     /locus_tag="VFH96_00500"
     CDS             10842..12020
                     /locus_tag="VFH96_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET6972465.1"
                     /translation="MRLNESVPQISLSELLRKLSELGGSDLHITTGSPPQIRVDGHLR
                     PLEQFRVLTSADTKQLAYSVLTDAQKHRFEENLELDFSFGVKGLSRFRANLFNQRGAV
                     GAVFRAIPYEIKAFEDLGLPAVVKQLCDKPRGLILVTGPTGSGKSTTLAAMIDKINRD
                     RHEHILTIEDPIEFLHNHKGCLINQREVNADTHGFAAALRTALRQDPDVVLVGEMRDL
                     ETIESALRIAETGHLTLATLHTNSASSTINRIIDVFPSDQQAQIRAQLSLVLEGILCQ
                     ALLPRAEGSGRAMALEIMVPNGAIRNLIREDKVHQIYSMMQTGQDKYGMQTFNQALAT
                     LYHKRQITLDTALQRSSNIDELRELIDRGAGINTNNGGKPHASPYAQGRPVGVRPNVR
                     "
     gene            12042..13271
                     /locus_tag="VFH96_00505"
     CDS             12042..13271
                     /locus_tag="VFH96_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6972466.1"
                     /translation="MPTFVFKGRNRLNEIVVGERVADNREALRQTLRREQVTLTSVKE
                     KGREIGIPKIGGRKKVKSKDLSIFTRQFSVMIDAGLPLVQCLEILAQQQDNKYFQQIL
                     LQVRQDVEEGSTLAAAMGRHPRVFDQLYSNMVEAGETGGILDLILQRLSTFIEKIVKL
                     KRDIISAMIYPAAVIVLAIVAVAVIMVVVIPQFQNIFLGLLGPGEQLPLPTRIVVGFS
                     NFLAGWGGLVILAVVIGVSVAIKFYYRTPAGRRNIDRILLKVPILGDIFRKIAVARFS
                     RTLSTLLSSGVPILQSLDITAKTSGNVIIEEAITKVRSGVERGESFVDPLKATEVFPH
                     MVAQMIGIGEQTGALDAMLGKIADFYEAEVDAAIANLLTLIEPLLIGFLGVTIGSIVI
                     AMYLPLFTLIGKLSTGH"
     gene            13595..16174
                     /locus_tag="VFH96_00510"
     CDS             13595..16174
                     /locus_tag="VFH96_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6972467.1"
                     /translation="MPKKRPATGSGNKVKNQLTNLQSPELAPYWLSALIESADDAIIS
                     KTLEGIITSWNAGAQRVFGYTADEVIGKPITILIPAGHEDEEPAILARLRAGERIEHY
                     ETIRVRKDGELINISLTVSPIIGPNGEIIGASKIARDVTEQRHARRALDEASERLKLA
                     STVARLGDWSWDAKTDVVTLSETAADILGVPPSPTPTRTKMRELLSKEDREATKRAVE
                     KALAEHTDYDLEYRVKRPDQSEAWVSSKGRGIYDKDGSVLGMLGLVQDVSIRKSNEET
                     LREQAEALRTLNEIGKTISAELDLHNTVQAVTDAATELTDARFGSFFYNVLNDEGASY
                     MLYTLAGVPREAFAHFPMPRATDLFGPTFRGEGVVRIDNVKLDPRYGKNSPYYGMPEG
                     HLPVTSYLAVPVISRSGEVLGGLFFGHPEPGIFTERDEIIVSGLASQAAVAMDNARLY
                     ETAKKARAEAERAAAEKERLYKQAEESSRLKEEFLATISHELRTPLSAILGWARMLRL
                     GQLSPENSAKALDTIERNARAQAQLVDDLLDVSRIITGKLRMDVRPADPNSFIDAAVE
                     SVRPAADAKGVRMQKVVDTNPISIPGDPVRLQQVVWNLLSNAIKFTPRGGRVQIRSER
                     VNSHLEIVVSDTGQGIAAEFLPHVFDRFRQADQKTSRNHGGMGLGLAIVRHLVEMHGG
                     TVRANSDGEGKGATFTVMLPIAPLYQVDASGGRIHPGARDLLPPPDSADRLDGLRILA
                     VDDEPDTRELLKQGLEFLGAKVKVVGTAAEAIDSLRGSPPDILISDIGMPGTDGYDLI
                     RQVRALPPDRGGKVPAIALTAYTRVEDRLQALRAGYDMHVPKPVELAELVAVAASVSR
                     RHS"
     gene            complement(16171..16947)
                     /locus_tag="VFH96_00515"
     CDS             complement(16171..16947)
                     /locus_tag="VFH96_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET6972468.1"
                     /translation="MNIDAYLERINYNGPRDVNAETLRALQVAHLMSVPFENLSIHSG
                     EPIVLNEDALYTKIVEERRGGFCYECNGLFAGLLRALGFDVAMLAAGVARADGSFGPI
                     FDHMALMVTLNERWLADVGFGESFLEPLLLDTRADQQQGTRSFRLIEHDDYLALMRRN
                     NGEDWQPQYRFTLQPYTFSDYEDMCRFHQTSPDSHFTKGLICSRATEDGRITLSDMLL
                     ITTSGPQRLRDEKTLSSREEYDRILRDEFGVVMKNPVRFV"
     gene            complement(16958..17464)
                     /locus_tag="VFH96_00520"
     CDS             complement(16958..17464)
                     /locus_tag="VFH96_00520"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6972469.1"
                     /translation="MTAPKQTRENDLQKLRELVKDIDFCMLTTVDEGGDLHSRPMSSN
                     GDIDADGDIWFFTNASSHKVSEIAKLPKVNVSFADPDNQRYISVSGAAQLVRDRAKID
                     ELWRPEFKIWFPKGKDDPEVALLRVSLEKAEYWDSPGSTIGYALSFVSSLVTGKEPDL
                     GENRKVNL"
     gene            17662..17994
                     /locus_tag="VFH96_00525"
     CDS             17662..17994
                     /locus_tag="VFH96_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6972470.1"
                     /translation="MPKNKADLLQGTLDMLILKSLSLGELHGYGVIQRLRQLSDEMLN
                     VEQGSLYPALYRIEQKGWVSARWDVHETGREAKFYKLTRAGRKQLETEEASWDRLCLA
                     IAKIRQAT"
     gene            18048..>20037
                     /locus_tag="VFH96_00530"
     CDS             18048..>20037
                     /locus_tag="VFH96_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972471.1"
                     /translation="MKWFNILRDRIRALRQREDVINDIDREMRLHLELQVDANIRAGM
                     SPAEAREKAMRSFGNLNRAVDAAYDVKGGGIFETLTQDIRYGARMLAKHKAFTSIAVI
                     TLALGIGANTAIFSVVNELLLQPLPYRDAERITTVWEVTPEGRHQNTTSRTNFRAWRD
                     QNTSFQNMAAFTDQRLNLTGTGEPEELSVQLATPELFKILGVEPLLGRTFLADDDKAD
                     QPPVAVLSYALWQRRFGGQVSVIGQPITLNSETFTIIGVMPPNFQFHIKQRSGTGRPA
                     ELWSILPMPVGGGANQRGRFLGTVARLKDGVTVDQAAAELRTIHARLSDEAPQFNKNY
                     TAEVLPLREQFFGNVRRPLWLMLGAVGFVLLIACANVANLLLSLATSREKEIAVRAAL
                     GARRIRIVRQLLTESVLLALVGSVIGLGFAWLGIKALVAISPRDLVSLQTVGLNFTVL
                     GWTLGVSLLTGIIFGLAPALHVSRLNLNDSLKDGGKSESGQASGSRKLRNVLVVSEIA
                     LAVVLLCSAGLLIRSFLRLQQVDRGFTTDNVLTMVIRLPEARYREDPQLVQFFSQALE
                     RVRQLPTARSAGIVNFLPLYGGLGSSTGFKILGQPEPPPGQGPSTDVRVADSGYFGTL
                     GIPLLRGRNFSDSEQREAKHVILINEALARKHFPNQDPI"
BASE COUNT         4564 a         5755 c         5437 g         4281 t
ORIGIN      
        1 cgcgccgttg ctggttcgca ttcggactca cgctctgaaa gttgtcgaga tagaacagca
       61 tcgccgggct ctgggccgtc gcttgtaaca acgtcgagaa cttacccagc gcgttcggac
      121 gaatcgtgtc gcgatcgtaa gcgggcagca accagcgatc ggcgcctttg ttagcgaaga
      181 tgttgaagtg gttggtccaa aagtcgacca tcacttcctg aagctgtcgc tcgctgtaaa
      241 ccgcacgcag aattcgcgac gcttgcagct cggcgatgat gcgttgcggt cgctgcaaac
      301 cgttctcgcg gtaatactca tcgatcactt tccgattctt ttcgttatcg agcggattct
      361 tcgctggatc agctggcatt tcctccggca ccatgccctt tgcttgaagc tgtcgcagca
      421 gttgaccggg ttgaggatac ttctcgtaaa gctctgcggt ggtcatgttg agggttgaca
      481 gctcacgaag cctattttct gcaaccgtgt cggaaatctt ctcaggattg agctgctggt
      541 taatgtagtt ctccaggccc atcgccttca cgcgctcgac gtcgcccgga cgcgcaccga
      601 atccaagccg attgaggacg tggatgatgc gctgttgttc ggttaacgag cgctgcttct
      661 gagcgaagac aagcggctgc gtcaacagga tgagtagtat taatgcaacg gtctttctca
      721 tggtcactta gacgcagggc ctgggagatt ggccgaaaag aaaccacgga taacacggat
      781 gaaatccgcg gattgatccg tgttatctgt ggttactgat tccttgtttg cggtcgtgga
      841 taggaaaccg ggctcttatt cccatcctta tcgatcgatc tgaccccgaa aaagtagttg
      901 tctttcgaca tccccttcat cgtaaaactc gtcacgtttc cgacaggctt cgaatcagtc
      961 cacacagcct cagtcgtctc gcgccaaacg atctcataac ccgcgagatc cggctccttg
     1021 ttcgcatccc aacgcaactc cgtgtcattc gacagacgct gcgccacgat cgtcacgcct
     1081 gccggtcgcg aaggcgccca cgctagcgcc gccagcgacg ccgcattcac gcgggccaca
     1141 ttcaccacat acccgaaatc aacaaactcc ggcaggtcgc caaactgcac gccattctcg
     1201 acccgtacgt tctgatgctg gtgccggaac tcctcgttta cctccgtgaa ccgcactgcc
     1261 ggatatccac gctccagaaa cggaatgtga tcaccgccgc gcccgtaacg atcgcgccgg
     1321 tagatcatcg tcactttgaa ctgcggcaca aacgcgtcgc cagtctcctt gatgaatcgc
     1381 gccagttgcc gcgacgcgga atcgttctcg ccaccaacac ttcgccgcgt cgtcgcttca
     1441 gccggcgttt catttgacgg cacgccttcg gagaacacac gcacgtgacc gcgatcgcgc
     1501 acgccattgc caccgagcga gttgccgacg atgtcgttgg tgaacatcgc gtcgacgttc
     1561 cagtttttct gtttggcctg ttcggcgaaa tgtgtcgagc cgagcaagcc ctgctcctca
     1621 cctgcaacca ccataaaaac aatcgtcgcg tcaaactcat acttcgccat cacgcgcgcc
     1681 atctcaagca cagcagcggt tcccgacgca tcatcgtttg cgccaggcgc gtcacacttc
     1741 gcatccgtgg gcgagttgca catcgagtcg tagtgaccac tcacgacata gatccgatca
     1801 gcagcctcgg gttgcgatcc tttcaacgtc gcgacgacgt tcgtgagcat cgtcggctca
     1861 ggaacgcgcg cggctttcgc ctgctcgtac gactgcttct cgacagtcat gcgaccgttc
     1921 gaggcttcgg ccgctttgag aaactcggca tatagccagt cgcgcgctgc gccgatgccg
     1981 cgcttcggat cgttctgttc tgacaacgtg ttgcgcgtac caaaggaaac gagctttctg
     2041 atcgtcgctt cgatattgcg cgcgtcgata tcgcgcacga tgttcgaaat ggcgcgatta
     2101 cgaacaggcg cagctctcct cgactgttgt ttggtttgag cggcaacaac gaggttcgcc
     2161 gccagtagaa aaatcattac gctttgaaaa actcggcaaa gttgatttcg aagcttcatg
     2221 gtgatctgta tttgatttta gcgtgttccg cgatcggcgg agtctctgta gatcggactg
     2281 aaggtgcggg tcgtgttttc agtaacgctt cccaatggtg gttccatgcg cggcggcagg
     2341 tgaggcatcg ccggtcggtc gttgaatgta ggccgaagtt gctgctgtgg tggtgcgatc
     2401 tcgcgctgca aggtcatcat cttcgttaag aaacggatga gtagaccaac gcagccgaac
     2461 agtgtggccg agctaaacag gaccatccaa acgatcgcgg cccaattcat attttgccgg
     2521 gcgaattccg tggcgctacc gaagatgacg cccatgccac cgagaatcgt tatgccaagg
     2581 ataatactgg gaacggtcag tttcggcggg gccaacatca ttggggcgct cgtcgtgggc
     2641 agcgttaagt tggcgccaca acgattgcag tatttaagct cgacagttat ctcgttaccg
     2701 caggtaggac aatacatagg acctccagtg cggtaactgt aacattaaag tgagccgcgg
     2761 atgaacgcgg atgaacgccg atctgcgttt atctgcgttc atccgcggct aattttttga
     2821 ctaacggatg cggtaggtgt gacctttcca gacgacgatg atctcgcctt cgagttggtc
     2881 tgcgatggtt ttgatcgccg gttcgtcggc cacgagtgcg cggtattgtt cagagccacg
     2941 actgagtgtc acggcatctt ttgaagaatc gtaagcagtg tcgacccaga caccgctttg
     3001 ttgacgaaaa cgtcgcccgg ctactgttcg cgtattcgac gttgcgtcat cgacgctacc
     3061 aatctgaagc tgtgcgcctt cacctgacgc tggcgcactc gctgcgtccg caggtttttt
     3121 cgcgcgggcc aaactgtctt tgtcatcccg ctgcgctccc cgaagctttc gagcctgtga
     3181 tccagcgacg ggttgcactt taacttcgtt cttggctgcc tcagtctcag cagcacgtgg
     3241 gctctgttcg ggagtagccg tcggctcaga tttcaccggc ggcggtgcgt tcgcggacgc
     3301 cgtttgttct tcaggcttcg gctgcgcgac tgctttgtct tgcgatacct gggcttcgac
     3361 actcgttact gacggcggca tattcggcgg cccagctggt tgcgctggtg cagtcttttc
     3421 cttcttctct tcgtcgactc gacctgggct cggtgagggg tttgaaacac tgaaaacgac
     3481 gcctgacgat gccgctggag atgcgaccgg ctgttgatga gtatcagcag cagtattccg
     3541 cgctacgtac gcttccggct gattacgacg caggaccaca aaaccgatag cggcaaccac
     3601 gatcagaccc aacgccggtg ctgcataacg caaaaccatc ggcgtgaaga gactcgctaa
     3661 aaacttcctg aaacctgacg gctctggaac tgcggcattg tctgttgtag ctacaacacc
     3721 agccgacgag ctgagttgcg caatgagact tcggcagcgc gagcactccg cgagatgtcc
     3781 tgtataacgc gcacgcgccg ccgcaggcag agcattctct gcatacgcgc tgagctcgtc
     3841 cgcatcgaga tgatcttcag gcacagctgc gtcgtcatga cgtccgagcc gtcgcagcaa
     3901 cagatccatc tcgttgtttg tttcctgttt cattgctttc ttaagaacgt aaccctctcc
     3961 ataccttgca tcaatccgcg ttcatcagcg caaatccgca gcttctttgc ttaaaatcga
     4021 ttccaaatcc atatctaaat gttgcgccac ctctgcaaat gaccgttcgg tttcagcctg
     4081 tgtccaacca cgttcatcaa cgaggatctt cgtgactcgt ttgcgcaaat cagtctgaat
     4141 cctcgtcaaa cgccggctcg cggtcgcttc gtgtacaccc agtacagcac ccgcctcacg
     4201 caatcgcagg ttgtcgaagt aataaagctt cactaacaat cgatcctctg cgggtaactc
     4261 ctgcacacac tggttgagcg cctgttgcat gtcagtctgc gcgaagcgat tcgaaatcga
     4321 ctcttccggc gacggtatgg cagcaacaat cggctggcct tccgcgacgc gcgcaaggcg
     4381 atcgagatcc gtgtcttctt ccgtttgcac gagccgcgac tgtttgcgaa acacgtctac
     4441 tgcaagttgg gccacaaccg cacgcagcca accgccaagc gagccgcgac ccgagtagta
     4501 ggcgagcttg ctggcgggca tgccatcctt gcgcgtgcgc agcccgtaga gttcagccca
     4561 aatcgactgc gcgagatctt cggcggcgtc ttcgttttta cttgccgcgc gcgcggcgga
     4621 acgcacggtc acagtgaatc gctcgaccag atcgttccag gccttctcgt caccacgctc
     4681 acaggcaatg atcaaacaca ggtcgtcagc ctgcaactcg tcgatgaatt tcgagatctc
     4741 tgcgtgaggc gtgtcggggt cgtcctttaa tagatacttc tcaaccgcgc cagtgatccg
     4801 cggaaccatg gcttcgcgac taagcgaacg attatcgctc gcccgcgcca gcatcctccc
     4861 agcgccatcg ttgagtattt tgtcgattcc agtattcacg gtgatccgtg tatttatccg
     4921 tggcaatccg tggccatcct accaccttgg cagtcatttg atgtagattc cacaactaca
     4981 gccaatgttg cgcgtcacag agatttttcg ctcgattcaa ggcgagtcaa cccacgccgg
     5041 acgcccgtgc accttcgtgc gcctgacggg ctgcccgatg cgttgtgtct ggtgcgacag
     5101 cgagtatacg ttcacgggcg gcgagcacta ctccatcgat aacatcatgg accaggtggc
     5161 ggcgttcgga tgcagactcg tcgaagtaac aggcggcgag ccgctcgcac aaaaggaagc
     5221 atttcagtta atccaaagac tctgcgacga cggctacgag gttctcatcg aaacgggcgg
     5281 ttatgtctcg accgcgaatg tagaccaccg ggcgaagatc attctcgata tcaagtgtcc
     5341 cgcttccggt gaagaacctc gcaacgactg gtcgaacctc gatcgcctcc gcgccgataa
     5401 agacgaggta aaattcgtaa tcgctgacga aggcgattgg atctacgcga agaacctgat
     5461 cgtagaacaa gctctggaaa gtcggacaaa agcagtcctg atctcgccag cctggggaca
     5521 agtcgatcta caacaactcg ctgactggat cgcgagcagc ggattgaacg tgcgcatgca
     5581 gttgcaacta cacaaataca tctggggacc tgacgttaaa ggagtgtgat gggcggctca
     5641 caggaaatcg aaactgaaga gctcgaaacg acgatagaaa acgatggacc acagctcgcc
     5701 atctgtctcg tttccggcgg catggactcg tgcgtcacag ccgcgatcgc caacgaagaa
     5761 aacgatgagc ttgcgtttct acacgtgtcg tacggacagc gcactgagaa acgcgagcgc
     5821 gaagcgtttg aagcactcgc cgatcattac aacgtgaaat cgcgcctcgt gatttcactc
     5881 gaacacctcg ctaaaatcgg cggctcctcg cttacggacg ctcgcattcc cgttaccaca
     5941 gcagatcttt cggtcccggg aattccctcg agttacgtcc cttttcgaaa tgcgcacctg
     6001 ctgtcagcgg ccgtaagctg gggagaagta atcaacgcca cgtcgatcta catcggcgcc
     6061 gttgccgaag actcgtcggg ttatcccgat tgccgtcccg agttttacga cgcttttcaa
     6121 aacgtgctcg acatcggcac gaagcccgaa acgcgcatca ctatcgaaac gcccgtgatc
     6181 gcgatgcgaa agtcggagat cgtgcgccga ggcatcgagc tgggcgcgcc gctgcacctg
     6241 acctggtcgt gttacaaaga gtcggaatcg gcctgtggga attgcgattc gtgcgccctg
     6301 cgactccggg catttcgcga ggccggcgtc accgatccca tcccttacct gcttgacagt
     6361 agtcgttttg cttattaagt ttagggctct cagagcgatt aattattaca accagtaaac
     6421 tgcgttggcg catcgtacga cggctgagcc aacacgacga cggaggaatc tttacatgat
     6481 tggcaaacat tttttcggag cgctagcggt agccattctc gttgcatctg cttcattgac
     6541 tgcggtcgcc caggtgggcg aactgcgtgg tcacatttgg atgcagcagg ctgatgggca
     6601 aaaggttccc cttgcggacg cgcagatcga cgtcttcagg accgacatga gcgcgaaata
     6661 caacaccaag acaaacaaaa aaggtgagtt cgttttcgcc ggactacctt tcatcggcac
     6721 gtacacagta gcggcgagcc atcccacggc ggcgccaaac ttccagcccg gcgtgaaagc
     6781 cggacgcgat atcgacgtcg agctcacgct gagtccgggc aatggaaagc gactcacgtt
     6841 cgaggagatc aagggtgctg gtggtggttc agcaacgcct ccgagtggcg gcacaccaca
     6901 acccagcgcc gaagataaag cgaagctgga ggagttgagg aaaaagaacg aagcgatcga
     6961 ggcctccaac aagaagatca cggaagcgaa cgaggtcatt gcgaaaacct tcaaggctgg
     7021 taacgaagcg ttgcaggccg cgggcgccgc gagcaaagcg aaccagtcag aggaagcgat
     7081 tgcgaagtac actgcggcag tagcccagta tgacgaaggt ctcgcagccg acgcggacca
     7141 gcccgcgatc ctgacgaaca aggcagtagc tttgaaggga cgcggcgtcg agaagttcaa
     7201 cacaaccgtc agatcgaaga ccctcgacga cgccggtcgc accgctggac tccaagccgc
     7261 gaaagacgac tttaaagccg cggcggaaac ctcggccaag gcggtgacga tgatcaaagc
     7321 cttgccggcg ccgaccgaag ctgcagattt gcaaagattc aacggtaaca agtacgccgc
     7381 gatgctgacg caagccgaat caatgcggct gtatgtgagt aaggctgatg gaacacaggc
     7441 tgatgccggc ctcgcagctt acaaggatta catcgcgatc gaaacggatc ccgcgaagaa
     7501 ggcaaaagcc caactcgata tggcgcagat gttgctcgat gcgggcgcct ctgacaaggc
     7561 actcacggag ttcaaggcaa ttctcgccga gaatccgaac agccctgaag ccaacctcgg
     7621 cgctggactg gcagtttacg cgacgggcga caaggcgaag tttcaggaag cagcgaattt
     7681 tctacagaag tttgtggaag tcgcgcctga cggcaacgcg atgaaggatg acgcgaaggc
     7741 gattctcacc gagttgaaga atagcgaaaa cgtctctccc gagaagacgt ccagacccgc
     7801 acgcaagaag ccataatcac ttagccacgg ataacacgga tgacacggat cgaaccgtgt
     7861 aaatccgtgt aatccgtggc tcaattcgtt agttttccta ccaaaactgc tggaaattcc
     7921 aaccttgaca cccttcttgt taaacttgta ctatgactgc acaacgatgt tagccccatc
     7981 gttgggatag ctagaccggt catgcctcgc gccacattcg gcgccaatcc accacccaga
     8041 gaatcgggga tctacctgct cccgagagct ttagtgcaca aatgtcagcc aaattaggcg
     8101 aaatattagt ccgcgaaaat ttaataagcc cgcagcacct gagagaggca ctcgactacc
     8161 agcgtgaaca tggtggtcga cttggcttca atctggtgaa gctcggtctt ataagcgacg
     8221 acatgattac tgccgtgcta tcacggcagt acggcattcc ctctgtcaac ttagatctct
     8281 tcaatatcga tccttccgtc atcagcctca ttcctcagga ggtcgcatca aaacactccg
     8341 tcctgccact ctcacgcgtt ggcgcaacac tcacgctcgc catggtcgac ccgaccaatg
     8401 ttttcgcgat ggacgacgtc aagtttatga ccggcttgaa cgtcgaaccc gtagttgtcg
     8461 ctgaaggcag tgtgcaacag gcaatcgcga agtattacgg cagctcacgc gagatcgagc
     8521 tcgccaccgt aaccaccgaa gaacttccca agaacggctc caagaacaac ggcgcaaacg
     8581 gcggtatcac gaaagacgat ctcgtttcgc tcgacagcat cgacttcgat cacaaccaga
     8641 gcgaagacgt tgaagtatta gaagataacg aagagatcga tctttcgaca ctctcgaaga
     8701 tgagtgaaga cgcaccagtc gtgcgtctca ccaacgtgct gctcgtcgat tcactgcgcc
     8761 gcggtgcttc tgacattcac atcgagccgt atgaaaaaga actgcgcatc aggtttcgta
     8821 tcgacggcgt gctttacgac gtcatgcatc cacccttgaa gatgcgcgat gcgttgatct
     8881 cgcgcatcaa gatcatgtcg aagctcgaca tctcggaaaa gcgtctcccc caggacggtc
     8941 gcatcaagat caaagtgaaa gtcgactcac gttcgcgcga gctcgacttt cgcgtttcta
     9001 cactcccgac tctcttcggc gagaaagtcg tgcttcgttt gctcgacaag caaaatctca
     9061 tgctcgacat gacgaagctc ggtttcgaac cggagtcatt aacgaaattc aagcgaaaca
     9121 tctccaagcc ctacggcatg gtgctggtga ctggtccaac tggaagcgga aagacaaaca
     9181 cgctttactc cgcgctccag tcattgaaca cagtcgaaac caacatcatg acggcggaag
     9241 acccggtaga gttcaacctt gctggcatca atcaggtgca gatgaaagag cagattggat
     9301 tgaacttcgc cgccgctctt cgatcattcc tacgccagga tcccaacatt gttctggtcg
     9361 gcgaaatccg cgacttcgaa acggctgaaa tcgccatcaa agccgcgctg actggtcacc
     9421 tcgtactctc taccctccac acaaatgatg ctccctccac catctcacga ctcatgaaca
     9481 tgggcatcga acccttcctg gtcgctacca gcgtgaacct catccaggct caacgtttga
     9541 ttcgtcgcgt ctgcaaggac tgcaagcagg aaacctcaat acccgccgag gcattcattg
     9601 aagttggttt cactatcgag gaagccaaga cgatgaagac tttcaaaggc aaaggatgtg
     9661 cgacctgcaa caacaccggc tacaagggac gaatcggact ttacgaagtg atggaaatca
     9721 ccgacgagct tcgtgaattg atcttgatcg gagcttccgc acttgaatta aggaagaagg
     9781 cgatcgacga cggcatgatc acgttacgcg aatcgggtct gcacaagatt cgcgcgggcg
     9841 tgaccacgat cgaggaagtc gttcgtgaaa cggtcgccta atcgaggaga acgaagatgc
     9901 ttagaccaat tgccatagcc ctgatcctgg tgctgtccgt aggcattatg ttgcccttcg
     9961 cgaacgaagc acacgggctc aggcaaactg tacaagttgg acagcgccgt catcaccggt
    10021 atcgttcacg cgcgtggtgg cgacgttatc atgcgcgcct gcgacagaag agattggctg
    10081 cggagttcgc cgcccatcgc aatgcaacac tgtcgttgcc gcaaaacctt tcggttgcgg
    10141 acctacaggg actcaacggt ccgcagttac cttcactgcc gtcaaactct gctgcgccgg
    10201 caaccctgcc gccgcccgag atgacagcag cgccggttgc ggctatttcg aatgcggcgc
    10261 atgcgtcgtc gctttcaaac ggcgaaaccc gtccgcgagt tgttaatcca aatcatcctg
    10321 ccttcaaagc ttcggcgaat gcgaccccac tgccgggaac gatgaatctc gcagtcatcg
    10381 cgatgagtcg accgaatccg gcgtttctga cttctcgtga ggagaagaag atgctgggcg
    10441 gtgtggctgt tgccgatatg cgacggatgg tgatcgacaa gatggtttcg tctggtggtt
    10501 gggtggtcaa tgacttcgtt cgcgaagtca acggcagtcg cgtgttcgta gttacggcgc
    10561 gaacgccgaa ggatgcactc acgccggaga aagcgtggac gttctatttc accgaagctg
    10621 gtggccgcat ctacgggctg accacggatg caccggttga gtttgccgag cgcatgacga
    10681 atgaagccga gcgcttcatc gaatcgttac gcgcgaaagc cgaagccggc acccgctaac
    10741 agcgtgcttt cttatccaca gattacgcag attgattaat aaatctgtgt aatctgtgga
    10801 tggattgctt ctttcaaacc ccgaccagga gaaacgcttc aatgcgactc aacgaatccg
    10861 ttccccaaat ctcactctcc gagctgctca gaaaactttc tgagctcggc ggttcggacc
    10921 ttcacatcac gaccggctcc ccaccgcaga tcagagttga cggacatctt cgtccgctcg
    10981 aacaatttcg cgtgctgaca tcagccgaca ccaaacaact cgcttactcg gtgttgactg
    11041 acgcgcaaaa gcatcgcttc gaagagaacc tggagctcga cttctcgttc ggcgtcaaag
    11101 gcctttcacg ctttcgcgca aacctcttca accagcgtgg cgccgtcggc gccgtgtttc
    11161 gcgcgattcc ctacgagatt aaagcattcg aagacctcgg cctgcccgca gtcgtcaaac
    11221 agctttgcga caagccgcgc ggactgattc tcgtcactgg tccaaccgga tccggtaagt
    11281 caactacact cgccgcgatg atcgacaaga tcaatcgcga tcgtcacgaa cacatcctga
    11341 cgatcgagga tccaatcgag tttctccaca accacaaagg ctgtttgatc aaccagcgcg
    11401 aggtcaacgc cgacacacac ggtttcgccg cagcgctccg caccgctcta cgtcaggacc
    11461 cggacgtggt cctcgtcggc gaaatgcgtg acctcgaaac gatcgagtca gcgttgcgta
    11521 tcgcagaaac gggtcacctt acactcgcaa cgctacacac gaactcagcc tcttcaacaa
    11581 tcaaccgtat catcgacgtc tttccttccg atcagcaggc acagattcgc gcgcagctct
    11641 cgctggtgct cgaaggaatt ctctgccagg cgctgctgcc gcgtgctgaa ggaagcggac
    11701 gcgcgatggc gctcgagatc atggtgccga acggcgctat tcgaaacctg atccgcgaag
    11761 acaaagtcca ccagatctat tcgatgatgc agaccggcca ggacaagtac ggcatgcaga
    11821 ctttcaacca ggcgctggcc acgctctatc acaagcgcca gatcacttta gacaccgccc
    11881 tgcaacgttc atcgaacata gatgagttgc gcgaattgat cgatcgcggc gccggcatca
    11941 acacgaacaa tggtgggaaa ccacacgcga gtccctacgc acagggccgt ccggtcggtg
    12001 tcagacccaa cgttagataa gccactctgg agcttacagc catgcctact tttgttttca
    12061 aaggccgcaa ccgactgaac gagatcgtcg tcggcgagcg cgtcgcggac aatcgagaag
    12121 cgctgcgtca gactttgcga cgcgagcaag tgacgctaac ctccgtgaag gagaaaggtc
    12181 gcgagatcgg cattccgaag attggcggac gaaagaaagt taagtcgaaa gacttgtcga
    12241 tctttacgcg ccagttttca gtgatgatcg atgcgggttt accgctcgtg caatgtctcg
    12301 agatcctggc ccagcaacag gacaacaagt atttccagca gatccttttg caggtgcgcc
    12361 aggacgttga ggaaggctcg actctcgccg ccgcgatggg ccgtcatccg cgcgtcttcg
    12421 atcagcttta ctcgaacatg gtcgaagccg gcgagacggg cggtattctc gatctgatcc
    12481 tgcagcgact atcgacgttc atcgagaaga tcgtgaagtt aaagcgcgac atcatctccg
    12541 cgatgatcta tccggcggcg gttatcgtgc tcgcgatcgt ggcggtggcg gtgatcatgg
    12601 tcgtcgtgat tccgcagttt caaaacatct tcctcggctt gcttggacca ggcgaacagt
    12661 tgccgttgcc gacgcgaatc gtggtcgggt tcagtaactt tctcgccggc tggggcggcc
    12721 ttgtgatact tgccgtggta atcggtgtct cggtggcgat caagttttat taccggacgc
    12781 ctgcgggcag gagaaacatc gatcgcattt tgctgaaagt gccgatcctc ggcgacatct
    12841 tccgcaagat cgcggtcgca cgtttttcgc gaacgctttc gacgttgttg tcttccggtg
    12901 ttccgatcct gcagtcattg gacatcacgg cgaagacctc gggcaacgtg atcatcgaag
    12961 aggcgatcac gaaagtgcga tcgggagttg agcgcggtga gagcttcgtc gatccgttga
    13021 aagcgacgga agtgtttccg cacatggtgg cgcaaatgat cggcatcggc gaacaaactg
    13081 gcgccctcga cgcgatgctc ggcaagatcg ccgacttcta cgaagccgaa gtcgatgccg
    13141 ccatcgccaa cctgttgacg ctgatcgagc ccttactaat cggcttcctc ggcgtcacca
    13201 tcggctcaat cgtcatcgcc atgtacctgc cgttgtttac gttgatcggc aaactttcga
    13261 caggtcacta gcctgcgaac cggacaaaac ttctagcccg cgaagcgggc gtgagcaaag
    13321 ctcgcgaagc gagcgacagc ataaagcctg gggcgcaagc cccaggataa cgatcaaaaa
    13381 aagcattgga agcccgcgaa gcggccgaca gcgcctgatg gttaaacgct gtcacccgct
    13441 tcgcgggctc gttcgctttc tttgatggct atcctggggc tggcgcccca ggctttatgc
    13501 tctcgcccgc ttcgcgggct gaaatgctat aatcctctca cccctgcgta cccctacagc
    13561 actctccttc gtacacgcgt gagatttgtg gagtatgccg aaaaaacgtc ccgcgaccgg
    13621 gtcaggtaac aaggtaaaga atcagctcac taatctgcaa agcccggaac tggcgccgta
    13681 ctggctctcc gctttaatag aatccgctga tgacgcgatc atcagcaaga cgctcgaggg
    13741 catcatcacg agctggaatg ccggagccca gcgcgtcttc ggttacaccg ctgacgaagt
    13801 aatcggcaag ccaatcacga ttctcatccc cgcaggccat gaagacgaag agccggcgat
    13861 cctcgcacgt ctgcgcgccg gcgaacgcat cgagcactat gaaactatcc gcgtcagaaa
    13921 agacggagag ctaatcaaca tctcattgac cgtctcaccg atcatcggtc caaacggaga
    13981 gatcatcggc gcgtccaaga tcgcacgcga cgtcaccgag cagcgacacg cgcgccgcgc
    14041 gctcgacgaa gcttccgaac gtctgaaact ggcttcgact gttgcacgac tcggtgactg
    14101 gtcgtgggat gcaaagactg acgtcgtcac gctatcggaa actgccgcag acattctcgg
    14161 cgttcctcct tcgccaacgc ccacccgcac caaaatgcgc gagctgctca gcaaagaaga
    14221 tcgtgaagcc acaaaacgag ccgttgagaa ggccctcgcc gaacacactg actacgatct
    14281 cgagtatcgc gttaagcgtc cggatcaatc tgaagcgtgg gtctcgtcaa aaggtcgtgg
    14341 aatctacgac aaagacgggt cagtgctagg gatgttagga ctcgtccagg acgtttcgat
    14401 tcgcaagtcg aacgaggaaa cgttgcgcga gcaggcggaa gcgctgcgca cactaaacga
    14461 gatcggtaaa acgatctccg ctgagctcga tctgcacaac acagtccagg cggttacaga
    14521 tgctgccact gaattgacgg acgcacgctt cggctcattc ttttacaacg tgctaaacga
    14581 tgaaggcgcg tcgtacatgc tctacacgct ggcgggcgtg ccgcgtgaag cgtttgcaca
    14641 ttttccaatg ccgcgtgcga ccgatctctt tggtccaact tttcgcggcg agggagtcgt
    14701 gcgcattgac aacgtgaaac tcgatccgcg ctacggaaaa aattcgccgt attacggcat
    14761 gccggaaggc cacttaccgg tgaccagcta tctcgcggtc ccggtgatct cacgctcggg
    14821 tgaggtgctc ggcggactgt tttttggtca tcccgaaccg ggaatcttta cggagcgtga
    14881 cgagatcatc gtttcgggtc tcgcttcaca ggcggctgtc gcgatggaca acgcgcgcct
    14941 ttatgaaaca gcgaagaagg cgcgcgccga agccgaacgc gcagcggccg aaaaggaacg
    15001 actgtacaag caagcggaag agtcgagccg tctgaaggaa gaattcctgg ctactatctc
    15061 gcacgagctg cgaacgccat tgagcgcgat tctcggttgg gccaggatgc tgcgactggg
    15121 ccagctctca cccgagaact ccgcgaaggc gctcgacacg atcgagcgca atgcacgcgc
    15181 gcaagctcag ctcgtcgatg acctgctcga cgtgtcgcgg atcatcaccg gcaagctgcg
    15241 catggatgtg cgtcccgcgg atccgaattc attcatcgat gcggcggtcg agtcagtacg
    15301 accggcggca gacgcgaaag gcgtgcggat gcaaaaagtg gtcgacacca atccaatctc
    15361 gattcccgga gatccggtgc gactgcaaca agttgtttgg aatttacttt cgaatgcgat
    15421 caagttcacg ccgcgtggtg gtcgtgtgca gattcgatcg gagagagtga attcacatct
    15481 cgagatcgta gtaagcgaca ccggtcaggg tattgcagct gagtttctcc cgcacgtgtt
    15541 cgatcgtttt cgtcaggcag atcagaagac cagtcggaac cacggcggca tgggactggg
    15601 tttggcgatc gtgcgacatc tggtcgagat gcacggaggc acggtgcgcg cgaacagtga
    15661 tggcgaaggc aaaggcgcga ctttcacggt gatgttgccg atcgctccgc tctaccaggt
    15721 cgatgcatcc ggcgggcgca ttcatcccgg cgcgcgtgat ctgctgcctc ctcctgactc
    15781 tgccgatcgc ctcgatggtc taagaattct tgctgttgat gacgaaccgg atacgcgcga
    15841 gctactgaag cagggcctcg agttccttgg cgcgaaagtt aaagtggtgg gaacggctgc
    15901 cgaggccatc gactcgctgc gaggatctcc tccggacatt ttgatcagcg acatcggcat
    15961 gccgggaaca gacgggtatg atttgatcag gcaggttcgc gctcttcctc cggatcgagg
    16021 cggcaaagtg ccggcaatcg cgctgacggc ttacacgcgt gtcgaagatc gtttacaagc
    16081 gttacgcgcg ggctacgaca tgcacgtgcc gaaaccggtg gaacttgcag agctcgtcgc
    16141 cgtcgccgcc agtgtgtctc gcaggcatag ttagacgaat cgcactggat tcttcatcac
    16201 tacgccgaac tcatcacgga ggatgcgatc gtattcttcg cggctcgata aggtcttttc
    16261 gtcgcgaagt cgttgcggac cggaggtggt aatcaagagc atgtcactga gtgtgatgcg
    16321 gccgtcttcg gtggcgcgcg agcagatgag acctttggtg aagtgtgagt caggcgatgt
    16381 ctggtggaaa cggcacatat cttcgtaatc ggaaaatgtg taaggctgaa gcgtgaaacg
    16441 atattgcggt tgccagtctt caccgttatt gcgacgcatt aaggcaagat aatcgtcgtg
    16501 ctcgattaaa cgaaacgatc gagttccctg ctgctgatcg gcgcgtgtgt cgagtagcag
    16561 aggctccaaa aacgattcac caaaaccgac gtccgccagc caacgctcat tcagcgtaac
    16621 catcagcgcc atgtgatcga agattggtcc aaagctgcca tcggccctgg ctacacctgc
    16681 cgcgagcatc gcgacgtcga atcctagtgc gcgcaacaag ccggcgaaca agccgttgca
    16741 ctcgtaacaa aacccgccgc gtcgttcctc tacgatcttt gtatacagcg cgtcttcgtt
    16801 aagaacgatc ggttcgccgg agtgaatgct cagattctca aaaggcacac tcatcaagtg
    16861 tgcaacctga agcgcgcgca acgtctccgc attcacgtca cgcgggccgt tgtaattgat
    16921 ccgctcgaga tacgcatcga tgttcatgcg ttggcgatca tagattcact ttcctgttct
    16981 cgccaaggtc cggctctttg cctgtcacca gtgacgagac aaaactcaac gcatatccaa
    17041 tcgtgctacc cgggctgtcc cagtactcag ctttttcgag actcacgcgg agcaacgcca
    17101 cttcgggatc gtctttccct ttgggaaacc aaatcttgaa ctcgggccgc cacaactcat
    17161 cgatcttcgc tcgatcgcgc acgagctgtg cagctccact caccgagatg taacgctgat
    17221 tgtccggatc cgcaaaactg acgttgacct tcggcagctt cgcaatctcg ctcaccttgt
    17281 gtgacgacgc gttagtaaaa aaccaaatgt cgccatcggc atcgatatca ccattcgacg
    17341 acatcggccg gctatgcaga tcgccacctt catcaaccgt cgtcagcatg cagaagtcga
    17401 tatctttaac cagctctcgc agtttttgca ggtcgttttc gcgcgtttgt tttggtgctg
    17461 tcatatatcc cctcgctcga tacatttatt ttttacgaac taagaatttg gacacagaac
    17521 ttcacagatt gatgtcaaaa agcacaagct ctcttttgcg cttttttgtg gctaaacttt
    17581 ttgcggcatg ctttgacaat ctaggtatcg atgcgtatga tgcctacctt ttctaggtat
    17641 cgatagcgaa ggggattgca cgtgcccaaa aacaaagccg atctgttaca ggggacgctc
    17701 gatatgctca tactcaagtc gctctcgctc ggagagttgc acgggtatgg tgttattcag
    17761 cgcctgcgcc agctttcaga cgaaatgttg aatgtggagc agggatcttt gtatcccgcg
    17821 ctctatcgca tcgagcaaaa aggttgggtc tccgcgcgtt gggacgtgca cgagactggt
    17881 cgtgaggcga agttttacaa actcacgcgc gcaggacgaa agcagctcga gaccgaggaa
    17941 gcgagttggg atcgactctg cctcgcgatc gcgaaaattc gacaagccac ataaaagccg
    18001 atcacaaaag gcacaaaaga aaaaaaggca caaaggaagc attactaatg aagtggttca
    18061 acattcttcg cgatcgaatc agagctttgc ggcagcgtga ggacgttatc aacgatatcg
    18121 atcgtgaaat gcgtctgcat ctcgagctgc aggtggacgc aaacatcagg gcgggaatgt
    18181 caccagcaga ggcacgcgaa aaagcaatgc gcagttttgg caatcttaac cgggccgtcg
    18241 acgcggctta cgacgttaaa ggcggcggca tcttcgagac gctcactcaa gacattcgct
    18301 acggcgcgcg catgcttgcc aagcacaaag cgtttacatc gatcgccgtc atcacactcg
    18361 cgctcggcat cggcgccaac acggcgatct tctcggtcgt caacgagctg ctgctgcaac
    18421 cccttcccta tcgcgatgca gaacggatca caactgtctg ggaagtcacg ccggaaggca
    18481 gacatcagaa caccacttcg cgtacaaact tccgcgcctg gcgtgaccag aacaccagtt
    18541 tccaaaatat ggccgcgttc accgaccaac gtttgaacct gacaggcact ggcgaaccgg
    18601 aagaattatc agtccagtta gcaacgcctg agcttttcaa gatcctcggt gtcgagccgc
    18661 tactcggccg cacgtttctg gccgacgacg ataaagcaga tcaacctccg gtcgcagtgc
    18721 tcagttatgc tttgtggcaa cgtcgctttg gtggtcaggt gagtgtcatt ggccagccaa
    18781 taactctcaa tagtgagaca ttcacgatca tcggcgtgat gccgccgaat tttcaatttc
    18841 acatcaaaca acgttcgggt acgggcaggc ccgcggagct ttggtccatc ttgccaatgc
    18901 cggttggcgg cggcgcaaat cagcgtggaa gatttcttgg taccgtggcg cgcttgaagg
    18961 atggcgtgac tgtagaccag gccgcggccg agttgcgcac aatccatgcg cgactttcag
    19021 acgaggcgcc gcaatttaat aaaaactaca ccgccgaagt gttgccgttg cgcgaacagt
    19081 tcttcggcaa cgtgcgccgc ccactctggc tgatgctcgg tgcggtcgga ttcgtgttgc
    19141 tcatcgcctg cgcgaacgtc gcaaacctgc tgctctcact cgccacctct cgtgagaaag
    19201 agatcgccgt gcgagccgct ctcggcgcgc gtcgtattcg catagtccgg cagttactaa
    19261 cggaaagcgt gttgctcgcg ttggtcggaa gcgtaatcgg tttaggcttc gcatggctgg
    19321 gaatcaaggc actcgtagcg atcagcccgc gcgatctcgt cagtttgcaa acggtgggtt
    19381 tgaacttcac cgtcttaggc tggacgctcg gcgtgtcgct gctaactgga attatcttcg
    19441 gcctcgcacc ggcgctgcat gtgtcgcgtc tcaatctcaa cgactcactc aaagatgggg
    19501 gcaagtcaga atccggtcag gcgagtggca gccgcaaact acgcaacgtg ctcgtcgttt
    19561 ccgagatcgc gcttgcagtc gtactgcttt gcagtgccgg tttgctgatc aggagtttcc
    19621 tgcgactgca acaggtggac cgtggcttca ctaccgacaa cgtattgacg atggtgatcc
    19681 gtttgcccga ggcgcgttat cgagaggatc ctcaactcgt acaattcttt tcccaggcgc
    19741 tcgagcgagt acggcagttg cccaccgctc gctccgcagg aatagtcaac ttcctgccat
    19801 tgtacggcgg actcggctca agtaccggct tcaagattct gggccaaccc gaaccgccgc
    19861 cgggacaagg accatccacg gacgtgcgcg tggctgactc aggttacttc ggcactctcg
    19921 gaattccgtt gctgcgcggg cgcaacttct ccgacagcga acaacgcgaa gcgaagcacg
    19981 tgatcctgat taacgaagca ctcgcccgta aacacttccc caaccaggat ccgattg
//
LOCUS       DASSFQ010000015         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1648700, whole genome shotgun sequence.
ACCESSION   DASSFQ010000015 DASSFQ010000000
VERSION     DASSFQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648700"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFH96_00535"
     CDS             <1..924
                     /locus_tag="VFH96_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HET6972472.1"
                     /translation="RIEARVGTTLVGSLPVMVRIRKNANTLTTAERDRFVAAFAQLNN
                     QGLGRFADFRDMHTNVSSPQAHGAPGFLPWHRAYLLDLERELQAIDPSVALHYWRFDR
                     PSPNIFTRDYLGESDNVGTVLFSGTNPLQFWVTDGVPGITRRPFFNTNNATGVRTEAQ
                     TLALGTTYPNFRTMEGNPHGSAHTSFGGSISSIGTAARDPLFFLLHCNVDRLWAKWQQ
                     INNRFNSAVAASYDSGAWPIGHNLVDTMWPWNGITGPPRPPTAPGGTLAPSPVVSAPG
                     LSPRVRDSLDFQGKINAAACMGFDYDDVPFS"
     gene            951..1844
                     /locus_tag="VFH96_00540"
     CDS             951..1844
                     /locus_tag="VFH96_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972473.1"
                     /translation="MTTKSKSTKKVTAAKKKSPAARTPRAAFAVATNEKKTTAERAKA
                     FAEAPLQTIDSDKNLQASLDVLRDRQQPIKVRLAALQSLQAASFSVIEFEPHREDYLA
                     TLREIVDDPDEELRQRVLGILARENDAYVQQRLLDGLKDTSKALVPPEKALQLLSYDV
                     HAEAYPVAREIVKAPPSPEAKREALRLLSADASSAPVFEKLLRDKDEDRDIRQISAAA
                     LQAVKPKRFQEQARELVLDDKEYDDIQATALTALAQFGDQKAVAKDQKLMKRVDELGK
                     GKSAKVKKTAKRFLSRYGSEK"
BASE COUNT          462 a          565 c          591 g          398 t
ORIGIN      
        1 aggatcgaag cccgggtcgg cacgacgctg gtcgggtcgc tgccggtgat ggtgcggatt
       61 cgcaaaaacg ccaataccct gacgacggcc gaacgcgacc gtttcgtggc ggcgtttgcc
      121 cagctcaaca accagggcct gggccgtttt gccgacttcc gcgacatgca caccaacgtc
      181 agctctccac aggcacacgg cgcgcccgga ttcctgccgt ggcatcgcgc ttacctgctc
      241 gatctcgaac gtgagttgca ggccatcgat ccgagtgtgg cgctgcatta ctggcgcttc
      301 gatcgcccgt cgccaaatat tttcacacgc gattatctcg gcgagtcgga taacgtcggc
      361 acggtgctct tcagtggcac caatccgtta cagttctggg ttactgacgg cgtgccggga
      421 atcacccggc gcccgttctt caataccaac aatgcgactg gcgtacgcac cgaagcgcaa
      481 acactcgcgc tcggtacaac ttatccgaac ttccgcacca tggaaggaaa tccacacggc
      541 tcagcgcaca caagcttcgg tggttctatt tccagcatcg gcaccgccgc tcgcgatccg
      601 cttttcttcc tcttgcactg caatgtcgat cgactgtggg ctaagtggca acaaataaac
      661 aatcgattca attccgcggt ggcagcatcg tatgacagtg gcgcatggcc gatcggtcac
      721 aacctggttg acacaatgtg gccctggaac ggcataaccg ggccaccgcg cccaccaacg
      781 gctcccggcg gcacgctcgc gccatctcca gtcgtgagtg cgcctggtct gagcccgcgc
      841 gtgcgtgaca gtctggattt tcagggcaag atcaatgcgg ccgcctgtat gggattcgat
      901 tacgacgacg tcccattttc ctaatgtggt tatagcagga gatcgaaatc atgacgacaa
      961 agagtaagtc gacaaagaaa gtaacggctg caaagaagaa gagcccggca gcgagaacgc
     1021 cgcgcgccgc attcgcagtt gctaccaacg agaagaaaac tactgccgaa cgcgcgaagg
     1081 cgtttgcaga agcgccgctt caaaccatcg actccgataa gaatctgcaa gcgtcgctcg
     1141 acgtgcttcg cgatcgacaa caaccgatca aggttcggct tgccgcgctt cagtcgctgc
     1201 aggcagctag tttttcggtg atcgagtttg aaccgcatcg tgaagactat ctcgccacgc
     1261 tgcgtgagat cgtcgacgat ccggacgagg agttgcggca acgcgtgctg ggaattttgg
     1321 cgcgtgaaaa cgatgcctac gtgcagcaac ggctgctcga tgggttgaag gacacgagca
     1381 aagctctcgt gccgccggag aaagcgttgc aattgctgag ttacgacgtg catgcggagg
     1441 cttatccagt ggcgcgtgag atagtaaaag cgccgccgag tccggaagcg aagcgcgaag
     1501 cgctgcggct gctttcggcg gatgcgagct cggcgcctgt gtttgagaag ttgctgcgcg
     1561 ataaggacga ggatcgtgac attcgtcaga tctcggccgc cgcgttgcag gcagttaagc
     1621 cgaaacggtt tcaggagcag gcgcgcgagt tagtgctcga cgacaaggag tacgacgaca
     1681 ttcaggcgac ggcgttgaca gcgttggcac agtttggtga ccaaaaagct gtcgcgaagg
     1741 atcagaagtt gatgaagcgc gtcgacgagc tcggcaaggg gaagtcggcc aaggtgaaga
     1801 aaacagcgaa acgcttcctc agtcgttacg gcagcgagaa gtgatcgaat cgttacgacc
     1861 tatccacaga ttacacagag ataaatctgc gtaatctgtg gatctggtga tgaatgctgg
     1921 aactacagtc acaagagttt tgtgatctcg tcgctcgcat ttcgatcgat gagttggtcg
     1981 atctcctcca cgaagacaac cccgtatacg atcagc
//
LOCUS       DASSFQ010000016         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_457764, whole genome shotgun sequence.
ACCESSION   DASSFQ010000016 DASSFQ010000000
VERSION     DASSFQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457764"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..2849
                     /locus_tag="VFH96_00545"
     CDS             72..2849
                     /locus_tag="VFH96_00545"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET6972474.1"
                     /translation="MDTCMPRLWSVDDQAARLAELTSHDRELKEAPLRRDVRSLGRLL
                     GEVLKEQVGDKLFSAVEELRLLLIEHRELHAQPGHDVDSEQRLIGRAETIVSRLDIAE
                     AHGMAKAFAIYFELTNLAETNHRKRRRRAAQTSPELLAQPGSFLGTLRRMRDAGINRQ
                     QALEWLAQIEVILVFTAHPTEVARRTVLFKRQRIAAELEQLDRLPLTERESDKHEQAI
                     IAEITALWQTDEVRRRQPRVRDEIRMGLDYYPSVLFETLPGLYDELADNFREAYGEEL
                     PANALPRVLRFGSWIGGDRDGNPLVTPECTRDALQIARETILGYYIDRVDDLIWQITP
                     STYQVSVSDELKAALSAYEATVTAPDLNPKRHPPQEVYRRFLDYVLARVILARDDPRN
                     ANAYRKAAEFCADLNLARESLSAHGGERVARLLFDPLLRQVDTFGFYLHTLDVREHAS
                     VHERALLEFGSVVASTDSALPAAPSAETVKLLDSLRMIGNLKRDFPPMAIRSYVISGA
                     QAASDVTGLVSLAELSGMHVASRDGNDPGVMPVPLFESIEDLRNCPSVCRSLWTSSDY
                     ARLLDSWERRQEVMLGYSDSNKDGGMLTSTWEIFKAHRALHRVAAECDVKLTLFHGRG
                     GTVGRGGGPTHHAIIAQPQGAFTGHIKITEQGEVMNWKYSDTVLAERNLELMIAASLE
                     ALSRPGDVAVLDQESEAALGQMSSDAFAFYREKIAENDEVLTYFEEATPVKELEHMRI
                     GSRPTRRSGRRGLGDLRAIPWVFGWMQSRHVVPAWFGVGFALERFSVVSSSNLRLLEK
                     LIQRFPFFSELVANVEMGLAKADLSIARLYAGLVSDVGLRERVFSMLFEEFERTKAVV
                     LRLTGQTTLLEKNPVLARSIRLRNPYVDPLSLIQVELLRRKRGGEGNDDLNYALAATI
                     NGIAAGLRNTG"
BASE COUNT          588 a          716 c          938 g          644 t
ORIGIN      
        1 cggtgccttg gcaccgcttt ggtaaacgta ccaaagcgcc aacaagttgg cgcactccaa
       61 ggaggcgtgt tatggataca tgtatgccac ggctctggag tgtagacgat caagcggcgc
      121 gactcgccga actaaccagc catgatcgcg agttgaagga agccccgttg cgtcgtgatg
      181 tgcgttcgct gggccggttg ctgggcgaag tcctgaaaga acaagtaggc gacaagctgt
      241 tttcggcggt tgaagagttg cgactgctgt tgattgagca tcgcgagttg catgctcaac
      301 cagggcacga tgttgatagc gagcaacggt taatcgggcg tgcagagacg attgtcagtc
      361 ggttggacat tgccgaagcg cacggaatgg cgaaagcgtt cgcgatctat ttcgaactca
      421 caaatctcgc tgaaactaat catcgcaaac gtcgtcgtcg cgctgcccag acgtcgccag
      481 agttgctggc ccaacccgga tcgtttctcg gcacgttgcg tcgcatgcgt gacgctggca
      541 tcaatcgaca acaagcgctc gagtggctgg cgcagatcga agtcatactt gtgttcactg
      601 cgcatcccac cgaagttgcg cgccgcaccg tgttgttcaa gcgacagcga atcgccgcgg
      661 agctcgaaca gttagatcgt ctgccgttga ccgagcgaga gtcggataaa cacgagcagg
      721 caatcattgc cgagatcact gcgctctggc agacggatga agtgcggcgc cgtcagccgc
      781 gagttcgcga cgagatcagg atggggcttg actattatcc gagtgttttg ttcgagacat
      841 tgcccggctt gtacgatgaa ttggcagaca attttcgtga ggcttatggc gaagaattgc
      901 cggcgaatgc gttgccgcga gtgttgcgat ttggttcgtg gatcggcggc gatcgcgacg
      961 gtaatccgct cgtcacaccg gagtgcacgc gagatgcatt gcagatcgca cgcgaaacga
     1021 ttctgggata ctacatcgac cgcgtggacg atctgatctg gcagatcact ccttcgacgt
     1081 atcaggtttc agtctcagac gaactcaaag cagcgctgag tgcctatgag gcaactgtga
     1141 cggcgcctga tctcaatccg aaaagacatc caccacagga agtctaccgg cgatttttgg
     1201 attacgtgtt ggcgcgtgta atcctggccc gcgacgatcc gcgcaacgca aacgcgtatc
     1261 ggaaggctgc tgagttctgt gcggatctga atctcgcgcg tgaaagcctt tccgcacatg
     1321 gcggcgaacg ggttgcgagg ttgctattcg atccgctgtt gcgtcaggtt gatactttcg
     1381 gtttttactt acacacgctt gatgttcgcg aacatgcgag cgttcacgaa cgtgcgttgc
     1441 tcgagtttgg aagcgtcgtc gcctccaccg atagtgcact tcctgcggcg ccgtcggcgg
     1501 agacggtgaa gttgctcgac tcactccgga tgatcggcaa cctgaaacgc gattttccac
     1561 cgatggccat tcgaagctat gtcatcagcg gagcgcaagc agcgtcggac gtgactgggc
     1621 tcgtttcgct ggcggagttg agcggtatgc acgtggcgtc acgcgatgga aacgacccgg
     1681 gcgtgatgcc ggtgccgctt tttgagtcga ttgaggattt gcgtaactgt ccgtcggtct
     1741 gccggagttt gtggaccagt tctgactacg ctcgcttgct cgattcatgg gaacgacggc
     1801 aggaggtgat gctcggttat tccgattcaa acaaagacgg cggcatgctc accagcacgt
     1861 gggagatttt taaggcccat cgtgcgctgc atcgagtcgc ggccgagtgt gacgtgaagc
     1921 tcacgttgtt tcacggacgc gggggaacgg tcgggcgcgg tggtggccca actcatcacg
     1981 cgatcattgc gcagccgcag ggcgctttca cgggccacat caagatcacg gaacaaggcg
     2041 aagtgatgaa ctggaagtac tcggatacgg tgttggccga gagaaatctt gagttgatga
     2101 ttgcagcgtc gttggaggcg ttgtcgcggc ccggtgatgt agcggtgctg gaccaggaga
     2161 gtgaagcggc gctgggacaa atgtcttcgg acgcgtttgc gttttatcga gaaaagatcg
     2221 cagagaacga tgaggtgttg acctacttcg aagaagcaac tccggttaag gagttggaac
     2281 acatgcggat cggatcgcgg cctacgcggc gaagtggacg gcgcgggctt ggcgatttgc
     2341 gcgcgattcc gtgggtgttt ggttggatgc agagccggca tgtggtgccc gcgtggtttg
     2401 gcgttgggtt tgcgctggag cgcttctccg tcgtgagttc gagcaattta aggttgctgg
     2461 agaaactcat acagcggttt ccgtttttca gtgagttggt cgcgaatgtt gagatgggat
     2521 tggcgaaagc tgatctgtcg attgcgcgct tgtacgccgg gctcgtttcc gatgtggggt
     2581 tgcgagagcg tgtgttcagc atgctcttcg aagagtttga gcggaccaaa gcggtcgtgc
     2641 tgcgtttgac tgggcagacg acgttgctcg agaagaaccc ggtgttggcg cggtcgattc
     2701 ggttgcggaa tccgtatgtc gatccgttga gtttgattca ggttgagttg ttgcgccgca
     2761 aacgtggcgg tgaaggcaac gacgacctga actacgccct cgccgcaacg atcaacggga
     2821 ttgccgcggg gctaagaaac accggttaag agaggctggg ccaagggcgg ccacggaggg
     2881 ccgccc
//
LOCUS       DASSFQ010000017         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_457933, whole genome shotgun sequence.
ACCESSION   DASSFQ010000017 DASSFQ010000000
VERSION     DASSFQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457933"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFH96_00550"
     CDS             complement(<1..202)
                     /locus_tag="VFH96_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972475.1"
                     /translation="MSFDKFLTKVFGSSNQRFLKSIMPLVDKINSLEPSVKALSDDEL
                     RGRSAQFKERLQKAVADVTDKEE"
     gene            complement(248..1318)
                     /locus_tag="VFH96_00555"
     CDS             complement(248..1318)
                     /locus_tag="VFH96_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972476.1"
                     /translation="MSELFEKFEVNREPRWQVLAKLAGASLVLHLVFVWMVIYVPAFR
                     DTLNIAALIASTKFVDKDYEATQIGDDVQMVQLSDKFHYPEGYFAQPLAAGQVPGQSG
                     AVDPFAPKIISQASKESHVDPEASPSPSPSASPVASPSPSAAAVAQASPSASPLTKEQ
                     AQVELEKTAEQNNVTLPEENEINKKVLKDFATYANDLKNQGKLDFEKPFEIEIEAKLD
                     EKGKLKDANVTRRSGDENLVELFRKLVAALNDSGFLAYLQPISKDNPGATVKITVKQG
                     EKDVLASVASEASSTEQAESLAKALNNLLYFGATTRLGHDEEVLMKNTNATPDGKRVM
                     INFSMPRQSVIDMIKKNLEPGV"
     gene            1422..2252
                     /locus_tag="VFH96_00560"
     CDS             1422..2252
                     /locus_tag="VFH96_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6972477.1"
                     /translation="MRRITRKNWDKRRSRGRRPPEKSARNRDHGANEPATRIVSPSRE
                     TKRLLEGIGTPRKTPFQPDPFQLEALASLEYEDVLVTAPTGSGKTWIAREEIRRLIDS
                     GRRAWYTSPLKALTNSKYHEFIDEFGAERVGILTGDRKENAEAPLIVGTTEIYRNQLF
                     DSLRGGNEVSADLVVLDEAHYLADEDRGHVWEEAIILTPPRIRLLLLSATIGNAHEFA
                     VWMEEIRGVRCGVITGPMKRPVPLRSALLLPDKRLLPLMNEAGRLNPEIEALAADKHG
                     "
     gene            2249..>3002
                     /locus_tag="VFH96_00565"
     CDS             2249..>3002
                     /locus_tag="VFH96_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972478.1"
                     /translation="MKLRMPEMPSATLLAALDGYNLLPAIVFLPTRRRCDQAASEAAL
                     TRRDPNESRREARRDFMRAFVEQHPEVRGHRHWDTIIRSGVASHHAGHIPAWKLVIEK
                     LMSAGLLDAIFATATVAAGVDFPARSVVLTGADARTGSGWRTLSASEMQQMTGRAGRR
                     GRDNVGFVIAAPGLHQDPERIAQLLKAPPDPLVSQFRATYTTLLNLLDAYGSFTSVRE
                     IAERSFAYRDFARQMAQLERSRSESEEKIHNAL"
BASE COUNT          642 a          867 c          822 g          671 t
ORIGIN      
        1 gctcttcttt gtcagtcacg tccgcgacgg ctttttgcaa gcgctcttta aactgagcgg
       61 agcgtccgcg cagctcgtcg tcggaaagcg ccttcactga cggttcgagc gagttgatct
      121 tgtcgaccag cggcatgatt gatttcaaaa accgctggtt gctgctgcca aaaactttag
      181 tcaggaattt atcgaaactc atagctcaat tagtttagcc aatcagtaat aatctgtgaa
      241 tcgctggtta aacaccgggt tctaaatttt tcttgatcat gtcgatgacg ctctgccgcg
      301 gcattgagaa gttaatcatc actcgcttcc cgtcaggcgt cgcattcgta ttcttcatca
      361 acacttcttc atcgtggccc aaacgcgtgg tcgctccgaa gtacagcagg ttgttcagcg
      421 ctttcgcgag cgactccgcc tgttccgtcg acgaagcctc tgacgcaact gaagccagca
      481 cgtccttctc accctgcttg actgtgatct tcactgtcgc gccgggattg tctttgctga
      541 tcggctgcaa gtacgcgaga aagccactgt cgtttagcgc cgccaccagc ttgcgaaaca
      601 actcaacgag gttctcgtcg ccggatctcc tcgtgacgtt cgcgtccttc agtttgccct
      661 tctcgtcgag cttcgcttcg atctcgatct cgaaaggctt ttcgaaatcg agcttgccct
      721 ggttcttcaa atcgttcgcg tatgtggcga agtctttcag gaccttcttg ttgatctcgt
      781 tttcttccgg cagcgtgacg ttgttctgct cggcggtttt ctccagctct acctgtgctt
      841 gttccttagt caacggcgaa gcggatggac tagcttgagc gacagccgct gccgacggtg
      901 atggactagc aaccggcgaa gcactcggac tcggtgacgg cgaggcttca ggatcgacat
      961 gcgattcctt actcgcctgc gaaattattt tcggcgcgaa tgggtctaca gcaccagatt
     1021 gtcctggaac ttgaccggcg gcgagcggtt gtgcgaagta gccctcgggg taatgaaact
     1081 tgtcactcaa ctggaccatc tgcacatcgt cgccgatctg agtcgcctcg taatctttgt
     1141 caacaaactt cgtgctcgca atcagtgcgg cgatgttcaa tgtgtcgcga aacgcgggaa
     1201 cgtaaatcac catccataca aagacgagat gcaggacgag cgaagcgccg gcgagctttg
     1261 ctaaaacctg ccaccgcggc tcgcgattta cctcaaactt ctcgaacaac tcagacatcc
     1321 tgcaaaacct gtcttaaaaa cctccgggtt agacgccatt gtaactctca gggttcgcgt
     1381 tagcacttcg caggccacac tctgaaagtt agaatggctt cgtgcgccga attacccgaa
     1441 aaaactggga taaacgccgc tcgcgtggtc ggcggccgcc ggaaaaatct gcccgcaatc
     1501 gcgaccatgg ggctaacgaa ccagccacac gcattgtttc accgtcgcgc gaaaccaaac
     1561 gactgctcga gggcattggt acacctcgta agacgccttt ccagcccgat ccgtttcaac
     1621 tcgaagcact cgcgagtctg gaatacgaag acgtgctggt cacagcgccg accggcagcg
     1681 gcaaaacatg gatcgcgcga gaagaaatac ggcggctcat cgatagcggc cggcgcgcct
     1741 ggtacacgtc gccgctgaaa gcgctcacaa actcaaaata ccacgaattt attgacgaat
     1801 tcggggctga gagagttgga atactgacgg gcgaccggaa agaaaacgcc gaagcgccgc
     1861 tgattgtcgg gacgactgag atctatcgca atcagctttt tgactcgctg cgtggtggga
     1921 atgaagtcag cgcggatctc gtggtgttgg acgaggcgca ctatctcgcg gatgaagacc
     1981 gcgggcacgt ttgggaagag gcgattatct tgacgccgcc gcgcattcga ttgttgttgt
     2041 tgtccgcgac gatcggcaac gcacacgagt ttgcggtgtg gatggaagag attcgcggtg
     2101 tgcggtgtgg agttattacg ggaccgatga agcgtccggt acctttgcgc tctgcactgt
     2161 tgctaccgga taagcgattg ttaccgttaa tgaatgaggc tggtaggctc aaccccgaaa
     2221 ttgaagcctt agccgcggat aaacacggat gaagttacgg atgcccgaga tgccgtcagc
     2281 cacactgctg gccgcactcg acggttacaa cttgctacca gccatcgtct tcctgccgac
     2341 gcgccgtcgc tgcgatcagg ccgcctccga agccgcactc acgcgtcgcg atccaaacga
     2401 gagccgccgt gaggcgcgcc gcgatttcat gcgcgcgttt gtcgaacagc atcctgaagt
     2461 tcgcggtcat cgtcactggg acacgatcat tcgcagcggt gtcgcgtcgc accatgccgg
     2521 acacattccc gcctggaaat tggtgatcga aaaactgatg agcgccgggc tattggatgc
     2581 catattcgct acggcgacgg tggctgccgg tgtcgacttc cccgcgcgca gcgttgttct
     2641 gaccggcgcc gacgcacgca ccggcagtgg ctggcgtacg ctttcagctt ccgaaatgca
     2701 gcagatgacg ggacgcgccg gacgccgtgg ccgcgacaac gtcggattcg tcatcgccgc
     2761 tccgggtctt catcaggatc cggaacgcat cgcacagctc ttgaaagcgc cgccggatcc
     2821 gctcgtcagc cagtttcgcg ccacttacac aacgctgctc aacctcctcg acgcttacgg
     2881 aagtttcact tcagtgcgcg agattgctga acgcagcttt gcgtatcgcg acttcgcgcg
     2941 ccagatggcc cagctcgaac gaagccgcag cgaaagcgaa gaaaagatcc ataacgcatt
     3001 ga
//
LOCUS       DASSFQ010000018         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_603283, whole genome shotgun sequence.
ACCESSION   DASSFQ010000018 DASSFQ010000000
VERSION     DASSFQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603283"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFH96_00570"
     CDS             <1..888
                     /locus_tag="VFH96_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6972479.1"
                     /translation="VDLGVETLAGSITNADDNKRAVEGISEIYHLAGKVSREREDARE
                     MYDIHVEGTRLLCDAAKAAGVKSIVLASTSGTIAVTKDGEVIPDETYPVPLDLISRWP
                     YYASKAYQEMAALERFHGKGLRLVIMNPSLLLGPGDDRLSSTKVILDFMARKIGAVPT
                     GGVSFVDARDAARTFRVAMKKGRHGERYLLGSANWTFSKFFGRLERLTKVSSPWLALP
                     SRLAITGANLVASFFKQWDMASPVEPGAIEMAQYFWYLNCAKASRELGFKPRDPGETL
                     HDTVSYLRENFLGSNAFGK"
     gene            1037..3103
                     /locus_tag="VFH96_00575"
     CDS             1037..3103
                     /locus_tag="VFH96_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HET6972480.1"
                     /translation="MNKRPALLPFLFCIFLTANAQGPATLQLGTPIERPLKPGQTQEF
                     TLNLTENTFVQIQVEQRGIDAIIRVFAPNGKSVGEYDSPNGADGPEHVAFVALATGPY
                     QISVSPFNPSDTATGQIEIRLVEMREANDQELKASKNQEVVKAKGVALLVEMDGLIAE
                     IKSPLTRIKAQLQAAQLIWSSDEKRASKFFADATIGFKEYLASLDPTGENYPQQFQAI
                     TDMRFQIIQILTERDPDAALSFIYSSKPPPNPYEDRRFNAQHEGVLELAIANRIVRTD
                     PNRALQIARQTLKTHYSVNLISTVGQLRQKNPELAAQFASEIASKLINDKILTKVEAA
                     SVVINLLNFAQNSRRNAVLNPDVKTAQIPLLTDDQYRDLLQKAVTEALSFPRPSAGTF
                     SPERDAALTLLYGLRQYGPDINNVSGGGTAAVQKKILELNPEGVGGMQYQNLIGNNSA
                     DAALDGIEKAPSDQREQLYLQLANREASNGDIVRARQIVNDRVTNPHQRRQAFANIEQ
                     QEMYRSLSKGKVEDALRMIGNLKTPRERARQLTRIAEQIGPGQKRANAITFLEQAKSL
                     LGASGQAQDDDQMSALIEIARAFGKYDAKRAFEILDPLIDQVNDLCAAARTMQGFGPE
                     YYEDDELSLNANSVGQVVTRMSNTIGSLALMNFERARATSDRLALPEVRLRVYMDIAQ
                     QTIQAK"
     gene            complement(3168..>4265)
                     /locus_tag="VFH96_00580"
     CDS             complement(3168..>4265)
                     /locus_tag="VFH96_00580"
                     /inference="COORDINATES: protein motif:HMM:NF037521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HET6972481.1"
                     /translation="GLLNDAADSLAKDDEEFARYLRNRARDLLSNDYESGDASWVTGH
                     FKNLNAQIGSYETYDDELFGVKTFFSFCLFLTRPAETTALRQAMKGLQALEDSLPYQN
                     HKKVREDIPVGVYDVIADFGQSRGGNTATILPNESYVARRYGRTILLRANIMRDPNIF
                     ASTSGTLAAAIGEQQAKELTPDGNFYRTLWHEVGHYLGVDRTRDGRELDEALQDNSNA
                     MEEMKADLVSLFVAEALHKQGYYSDAQLRSVYAGGILRVLQNVRPRRDQPYQTMQLMQ
                     WNFYLENGLLTFDPATKLMQVHYEKYHDVVGKMLEKTLAVQYEGDKAASDRYIDQYTK
                     WDENLHGVIAANIRAQQRYRYRVFEYAALGE"
BASE COUNT         1021 a         1197 c         1148 g          899 t
ORIGIN      
        1 gtcgatctcg gtgtcgagac attagccggc tccattacta acgccgacga caacaagcgc
       61 gcggtcgagg gcatcagcga gatctatcac ctcgccggga aagtctcgcg cgaacgtgaa
      121 gacgcacgcg agatgtacga cattcacgtc gaaggtacac gcctactctg cgacgccgcg
      181 aaagctgctg gcgtcaagtc gatcgtgctc gcgtcaacca gcggcacgat cgccgttacg
      241 aaagatggcg aagtcattcc tgacgagacg tatccggtcc cgctcgacct catctcgcgc
      301 tggccttact acgcaagcaa agcgtaccag gagatggccg cgcttgaacg ttttcatggc
      361 aaaggtttgc ggctcgtgat catgaatccg agtctgctgc tcgggcccgg cgacgatcgg
      421 ctcagctcga ccaaagtcat tctcgacttc atggcccgca agattggcgc cgtgccgacc
      481 ggcggtgtga gttttgtcga cgcgcgcgat gctgcccgca cgtttcgagt ggcgatgaaa
      541 aaaggccggc acggtgagcg ctacctcctc ggttctgcaa actggacctt ctcaaaattc
      601 ttcggacggc tcgaacgcct cactaaagtc tcgtcgccgt ggctcgctct gccgtcaaga
      661 ctcgcgatca cgggtgcgaa tctcgtcgct tcgtttttca agcagtggga catggcgtcg
      721 ccggttgaac ccggcgcgat cgagatggcc cagtatttct ggtatttgaa ctgcgcgaaa
      781 gcgagtcgcg agctcggctt caagccacgc gatcccggcg agacgcttca tgataccgtc
      841 agttatctca gggaaaactt cctcggcagc aacgctttcg ggaaatagcc acaaaaagca
      901 caaaaagaaa gttaaggcgc agtctgaagg ctgaactcta aactttttct tttgtgcttt
      961 ttgtgccttt tgtggctaag ctttagggaa ccgtttggta gtctcgggcg tcttaggaca
     1021 gtcaggagat ctcatcatga acaagcgacc cgcactcctt cccttcttgt tttgtatctt
     1081 cctaaccgca aatgcccagg ggccggcgac gctgcaactc ggcacaccga tcgaacgccc
     1141 actcaaaccc ggacagacac aggagttcac actcaatctc actgagaaca cttttgttca
     1201 aatccaggtg gagcagcgtg gaatcgacgc gattatcaga gtgtttgcgc ctaacgggaa
     1261 aagcgtcggc gaatacgatt caccaaacgg cgctgatggg ccggagcatg ttgcattcgt
     1321 agcactggcg accggccctt atcagatatc tgtgagtccg tttaacccca gcgacaccgc
     1381 gacgggacaa atcgagatca gattagtcga aatgcgggag gctaacgacc aggagctcaa
     1441 ggccagtaag aatcaggaag tggtgaaggc aaaaggcgtc gccttgctcg tcgagatgga
     1501 cggtctgatc gcagaaatca aatcgccgct aacgcggatc aaagctcaac ttcaggccgc
     1561 gcagctcatt tggagctccg atgaaaagcg cgcctcgaag ttttttgctg atgcgacgat
     1621 cggattcaaa gagtatttag cgtcgctcga tcccactggg gagaattatc cacagcaatt
     1681 ccaggccatc accgacatgc gcttccagat catccagata ctcacggaac gcgatccaga
     1741 tgcggccctc agttttatct attcgagtaa accgccgccg aatccttacg aagaccggcg
     1801 ctttaacgcg caacacgaag gtgtactcga gctggcgatc gccaatcgga ttgtgcgcac
     1861 cgatcccaac cgcgcgctac agatcgcgcg acagacgttg aagacacact actcagtgaa
     1921 tttgatcagc accgttggtc aactccggca aaagaacccg gagttggcgg ctcaattcgc
     1981 aagcgagatc gcgagcaagc ttattaacga caagatactc accaaagtcg aagccgcttc
     2041 agtggtcata aacctgctta acttcgccca gaattcacga agaaatgcag tcttaaatcc
     2101 cgacgtaaaa acagcgcaaa tcccacttct gactgacgac caatatcgag acctgctaca
     2161 aaaagccgtc acggaagctt tgtctttccc tcgaccatca gcgggcacgt tttcaccgga
     2221 acgagacgct gccttaacct tgctttacgg attgcgacag tatggaccag acatcaacaa
     2281 cgtctcgggt ggcggcacgg cagcagtgca aaaaaagatt ctggaactga acccggaagg
     2341 tgtgggtggg atgcagtatc agaatctcat cggcaacaac tcggctgatg ctgcactgga
     2401 cggaattgag aaagcgccgt cagatcaacg agaacaactc tacctacagt tagccaatcg
     2461 cgaagccagc aacggcgata tcgtacgggc cagacagatc gtgaacgatc gggttaccaa
     2521 tccgcaccag cgccgccagg cgttcgcgaa tatcgagcag caggagatgt accgctcgct
     2581 ttcgaaaggc aaggttgaag acgcactgag aatgatcggc aacctgaaaa cgccgcgcga
     2641 gcgtgccagg cagctaacgc ggattgcgga gcagatcggg cccggacaga aacgcgcgaa
     2701 cgcgataact tttctcgaac aggcgaagag tttgttgggt gcgtcggggc aagcgcagga
     2761 tgacgaccag atgagtgcct tgatcgagat cgcgcgtgcg tttgggaagt atgatgcgaa
     2821 gcgcgcgttc gagatactcg atccgttgat cgatcaggtg aatgatctgt gcgcggcagc
     2881 gcggactatg caaggctttg gtccggagta ttatgaagac gacgagctga gcctgaacgc
     2941 gaacagtgtg gggcaggtag taacgcgcat gtcgaatacg attggatcgc tggcgctcat
     3001 gaacttcgag cgcgcgcgag caacgtcaga ccggctggca ctgcccgagg tgcgattgcg
     3061 ggtctacatg gatatcgcac agcaaacgat ccaggcaaaa tgagccacgg atttacacag
     3121 atgaacacgg ctcaatcagt gtttatccgt gtaatccgtg gctaattcta ttcgcctaat
     3181 gccgcgtact cgaaaactct gtagcggtag cgctgctgag cgcgaatgtt cgcagcgatc
     3241 acgccgtgca ggttctcgtc ccacttcgta tactgatcga tgtatcgatc ggaagcggcc
     3301 ttgtcacctt catactgcac cgcaagcgtc ttctcaagca tcttcccgac cacgtcgtga
     3361 tacttctcgt agtgaacctg catcaacttc gtcgccggat cgaatgtcag taacccattc
     3421 tcgagataga aattccactg catcaactgc atcgtttgat aaggctgatc gcgccgtggt
     3481 cgaacgttct gcaacactcg cagaatgccg ccggcgtaga cactgcgcaa ttgcgcatca
     3541 ctgtaatacc cctgtttgtg cagtgcttca gcaacaaaca gcgataccag atccgccttc
     3601 atctcttcca tcgcgtttga attgtcctgc aatgcctcgt cgagctcacg gccatcgcgc
     3661 gtgcgatcca cgccgaggta gtggcccact tcgtgccaca acgtgcgata gaaattaccg
     3721 tcaggcgtca gctcctttgc ctgctgttcg ccgattgctg ccgcgagtgt tcccgacgtg
     3781 cttgcgaaga tgttcggatc gcgcatgatg tttgcgcgta acaggatcgt ccggccataa
     3841 cgacgcgcca cgtaactctc gtttggcaga atcgtggcag tgtttccacc acgcgattgt
     3901 ccgaagtctg cgatcacatc gtagacgccg actggtatgt cttcacgcac cttcttgtga
     3961 ttctggtaag gcagcgagtc ttcgagtgcc tgcaatcctt tcatcgcctg gcgcagtgca
     4021 gtcgtctcgg ctggtcgcgt caaaaacaag cagaacgaaa agaaagtctt cacgccaaac
     4081 aactcgtcgt cgtaagtctc gtaagatccg atctgcgcgt tgagattttt gaagtgtccg
     4141 gtgacccagc tcgcgtcacc cgactcgtaa tcgtttgaca acagatcgcg cgcgcgattg
     4201 cgcaggtaac gcgcaaactc ctcgtcatct ttcgcgagcg aatcagccgc atcgttcaag
     4261 agacc
//
LOCUS       DASSFQ010000019         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1349531, whole genome shotgun sequence.
ACCESSION   DASSFQ010000019 DASSFQ010000000
VERSION     DASSFQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349531"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..857)
                     /locus_tag="VFH96_00585"
     CDS             complement(117..857)
                     /locus_tag="VFH96_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972482.1"
                     /translation="MSPLEHFCEALAKARVELDPFPHFYLENVFPDDYYQELLRNLPS
                     TDVYDNLYEVTDLKLDHFRHRNQRDMDAGWIDRLPKDLQPFWRAFNDWFLGPDLAQAV
                     LDSFSSQRSSWPEVSVESQFIRHRAGYFLGPHSDLYTKLVVLLIYLAPDNSAEHLGTS
                     LYRPKDPSFSCPDSKHYPFADFIRVKTAPYKPNSMLVFMRSDISFHGLEPLSQTDVAS
                     EGRDVIQYVLHDKQVREAQLRAKERATT"
     gene            complement(854..1933)
                     /locus_tag="VFH96_00590"
     CDS             complement(854..1933)
                     /locus_tag="VFH96_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6972483.1"
                     /translation="MSLRFHWMMPKGGEVGMKTAQETSRVATTQAHSPAALPDMEGWL
                     QFARAAEEAGIESVLLSFSRYEPDTIFVACAMGQVTTKLGFIVAYRLGLMQPATFVQQ
                     INTLSGLINGRVSLNVVAGSSAVEQRGYGDYLDHDERYARAEEFLTVCHAFWADNGAV
                     SFSGKYCHVDKGKIFTPFLAPDRSEPEIYVSGHSEQAQHLALTQASCYLRLIDTPEKL
                     APIVGHFRENGVEVCLRLCVICRPTMDHALAAAQALMPAEEIEKQERKILSSSDSQTL
                     KEALRAADDVGWMNRNLYAGLVPYYGSSAITLVGTPEDLARAFMEYKEIGVTQFIIAG
                     WPKLDEMVIFGREVLPLVRELEQKA"
     gene            complement(1930..>2742)
                     /locus_tag="VFH96_00595"
     CDS             complement(1930..>2742)
                     /locus_tag="VFH96_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6972484.1"
                     /translation="LKFIIAYRSGLMQPTTFVQQMNTVSALVQGRVSFNIVAGSSSEE
                     QRGYGDYLSHDERYARAEEFLAVCNAFWKANGEVNFEGKYYRVEQAKLATPFVAPDKQ
                     APEIFVSGHSEQSEHLASSQGTCWLRVAESPSKLEPIVARTRARGTGVCLRCCLICRP
                     TRAEAIEAAQSLLPEDAAESTTTMKDDSQMYREGNELKRDAFWPEPWLWTGLVPHYGP
                     VWTTMIGTPDELASAFLEYKRIGVTEFIISGWPEVDTVDVFGREILPRINQA"
BASE COUNT          599 a          856 c          721 g          566 t
ORIGIN      
        1 ccctacagtt gcttccgaga tacctaataa ttccacgccg tctaaatttt tgagcgcgtt
       61 agcgctgcgc agactttctc cagtcgcact ccgacagacg aacacgacgc gccggctcat
      121 gtcgtggccc tctctttcgc gcgtaattgc gcctcgcgca cttgcttgtc atgcagcaca
      181 tactggatca catcgcgccc ctcgcttgcg acatccgtct gcgacagcgg ctcgagtccg
      241 tgaaacgaga tgtcagagcg catgaagacc agcatcgagt tcggtttgta cggcgccgtc
      301 ttcacgcgaa tgaaatctgc aaacggataa tgcttgctgt cgggacagct aaacgacgga
      361 tccttcgggc ggtagagcga agtaccgaga tgctcggcgc tgttatcagg cgccagatag
      421 attagaagca ccacgagctt tgtataaagg tcggaatgtg gaccgaggaa gtaaccagcg
      481 cgatgccgga tgaattgcga ctcgacagag acttccggcc acgacgatcg ttggctgcta
      541 aacgaatcga gcactgcctg ggcgagatca ggaccgagaa accagtcatt aaaagctctc
      601 cagaatggtt gcagatcttt cgggagtcga tcgatccagc cggcgtccat gtcgcgctgg
      661 ttgcgatggc gaaagtgatc gagtttcaga tccgtgactt cgtagagatt atcgtagaca
      721 tctgttgaag gtagattgcg taacaactcc tgatagtagt cgtcaggaaa aacgttttcg
      781 aggtagaagt gtggaaacgg atcgagctcg acgcgggctt tcgctaaagc ttcacaaaaa
      841 tgctccagtg gactcatgct ttttgctcca gctcacgcac gagcggcagc acttcgcgcc
      901 cgaagatcac catctcatcc agcttgggcc agcccgcgat gatgaactgc gtcactccga
      961 tctctttgta ctccatgaac gcgcgcgcca gatcctcagg cgttcccacg agcgtaatcg
     1021 ccgatgaacc gtagtagggc acgagccccg catagagatt tcgattcatc caacccacgt
     1081 cgtccgccgc ccgcaacgcc tccttcaacg tctgcgaatc gctgctcgac aaaatcttcc
     1141 gctcctgctt ctcgatctct tcagccggca tgagcgcctg cgcggccgcg agcgcgtggt
     1201 ccatcgttgg cctgcaaatc acacacaacc gcaaacaaac ttccacacca ttctcgcgaa
     1261 agtgcccgac gatcggcgcc agcttctccg gcgtgtcgat caatcgcagg taacacgatg
     1321 cttgagttaa tgcgagatgc tgcgcctgct ccgaatgtcc tgacacgtaa atctccggct
     1381 cgctgcgatc cggcgcgaga aacggcgtaa agatctttcc tttatcgacg tgacaatact
     1441 tgccggagaa actcacagcg ccattgtcag cccagaacgc gtgacacaca gtcagaaact
     1501 cttccgcgcg cgcgtatcgc tcatcgtggt ccaggtaatc tccgtacccg cgctgttcca
     1561 ccgccgagct gcccgccacg acattcaaac tcacacgccc attaatcaat cccgacaacg
     1621 tgttgatctg ctgcacgaac gtcgccggct gcatcaaccc cagacgatac gcgacgatga
     1681 acccgagttt cgtcgtcacc tggcccatcg cacacgcgac aaagatcgtg tcaggctcgt
     1741 agcgactaaa cgacagcaac accgactcga tgcccgcctc ctccgcagca cgcgcaaact
     1801 gcagccagcc ttccatgtca ggcaaagcgg cgggcgaatg cgcctgcgtc gtcgctacac
     1861 gcgaagtttc ctgcgcggtc ttcatgccga cctcgccgcc cttcggcatc atccagtgaa
     1921 atctcaggct catgcttgat taatgcgtgg caatatctcg cgtccaaaaa catctaccgt
     1981 atccacttcc ggccaaccgg aaatgatgaa ctccgtcacg ccgatccgtt tgtattcgag
     2041 aaacgccgac gccagctcgt ccggtgtgcc gatcatcgtg gtccataccg ggccatagtg
     2101 cgggaccagg ccggtccaga gccacggctc gggccaaaaa gcgtcgcgtt ttagctcgtt
     2161 gccttcgcga tacatctgcg aatcgtcctt catcgtcgtc gtgctttcgg cagcgtcttc
     2221 gggcaacaaa ctctgcgcag cctcgatcgc ttctgcacgc gtgggccggc atatcaaaca
     2281 acaacgcagg cagacccctg ttccacgcgc gcgcgtacgc gctacgatcg gttccaactt
     2341 cgacgggctc tcggcaacgc gcaaccagca agtgccctgc gacgaagcga gatgctccga
     2401 ctgttcggag tgccccgaca cgaagatctc cggcgcttgc ttgtccggcg cgacaaacgg
     2461 tgtcgcgagt tttgcttgct cgacccgata gtatttgcct tcgaagttga cctcgccatt
     2521 cgctttccag aaagcattgc agacggcgag aaactcctcg gcgcgcgcgt accgctcgtc
     2581 gtgtgaaaga tagtcgccgt aaccgcgctg ttcttcactc gagctgccgg caacgatgtt
     2641 gaatgacacg cgcccctgga cgagtgcgga gacggtgttc atctgctgga cgaaggtcgt
     2701 cggctgcatc aggcccgagc gataggcgat gatgaacttg ag
//
LOCUS       DASSFQ010000020        18994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_757010, whole genome shotgun sequence.
ACCESSION   DASSFQ010000020 DASSFQ010000000
VERSION     DASSFQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18994
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757010"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFH96_00600"
     CDS             <1..193
                     /locus_tag="VFH96_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972485.1"
                     /translation="QPGVDIFACTHVLAHLGLGRALAINGDTAGARKAYQDFFALWKD
                     ADHDLPVLVDARKEYETLK"
     gene            323..1066
                     /locus_tag="VFH96_00605"
     CDS             323..1066
                     /locus_tag="VFH96_00605"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6972486.1"
                     /translation="MSGHSKWHSIKHKKGALDAKRGKLFTKFIKEITVAARTGGGDPD
                     ANARLRKAILDAKAGNMPNETIDRAIRRGTGEEEGVNYEEITYEGYGPGGVALLIQSM
                     TDNRNRTVAEIRHLFSKNGGNLGESGSVGWIFEKKGYIVVSKSDKSEEELFDLVIEAG
                     ADDLRDDVDNFEIITSPEAFEGVQAAVKGAGIEPQVAEVEFVPQNYIKLEGSDARQML
                     KLMEALEDHDDVQKVSANFDISEADMAAV"
     gene            complement(1137..9899)
                     /locus_tag="VFH96_00610"
     CDS             complement(1137..9899)
                     /locus_tag="VFH96_00610"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET6972487.1"
                     /translation="MSEFIESVSSFLGKPTGYADEKPFQAEFFSVERLEQYAQTLAAE
                     HKTVTRKGRAQLLPRLEDNGRKLEAAYKALVNALREGRAISPAAEWLVDNYHIIEEQL
                     REIRQDLPTSYYHELPKLAEGELADYPRIYAVALALIAHTDSRLDTNTLQRFIASYQT
                     VAPLSIGELWAVAITLRLALVENLRRLAIAIARARLERDEADKLADKLLETASLEPAN
                     VMSSLNDRLGKREDLPHAFLVQLVQRLREQHPSVMPVMDWIEKEVANQGTTVEQIIHS
                     EHQRQAAAQVTVGNIITSMRLLSTLDWNDFFETVSLIERLLGNDPAGVYAQMEFAARD
                     RYRHVIERISKRTRTNELDIGQAAIDLAAHANERSRAQRHVGYYLIDAGLNQLETKFR
                     YQPGTTERLRRFLLRHCTTTYLGTLTVMTFLIMTPLLYFMSRYRVGWPFLILTALLAL
                     IPASDLAITVLNWDLTHFFPPRLLPRMNTSDGIPDDAATVVVVPTIFLNDSQVHELVE
                     RLEVHYLANQDEQIYFALLSDFPDADAEQTPADSSLLAIAQSGIDALNRNHGNERFHL
                     FHRKRQWNAGEHKWMGWERKRGKLEEFNRLLRGAQDTSFVVRTANDALLKSIRYVITL
                     DSDTSLPRDVARKLVGAAIHPLNQPKIDEATNRVTRGYGILQPRVSIALASASRSKFV
                     QVFSGYTGIDPYTTAVSDVYQDFFGEGNFTGKGLYDVDAFQTTLENRVPPNSLLSHDL
                     FESLFARAALTTDIELLDDYPASYEAYAKRSHRWVRGDWQISRWLFPGVPDGNRKKVH
                     NTLPLISRWKILDNLRRSLVAPALFLWLVASCALFPGSALLWSLFVFLVIAFPVYLHV
                     TTGLAIHPRGIPWTSHFWSRWGDFRTNTAQIALSFVFLPHQAWLMCDAIVRAVYRQLI
                     SRRKLLEWVSAAEAESSLRNNLQSFVRFMLPALILTVAALGLTLSFKPRALPEISTLF
                     VIWILSPLIAYFVSKPRPAERKLLNEEDKAFARLIARRTWRFFETFVGPEDNWLPPDN
                     YQEDPSPVVAHRTSPTNMGLLLLGNHSARDLGYLGALELLERQELTFATLQKLGRMHG
                     HYFNWYDTKTLEPLLPQYISTVDSGNLAGHLIAVKQACIEFPETRLFDVHVIEGLTDT
                     INAIDTEASALGSFRQRTDVVTVRQLHDEIAACRALLQVDPAESLSSWIALFDSLGRR
                     ASEIEDIVNALVHEHGEVSFKELRWWVGALQHQVKSLRRDAESLTGWGRLLSSVENEI
                     NSAEWIKLLQQVPALAEIPQLCDKALVQLAATHADASADASKLTKALEQSAGASSDML
                     SRLSRLARRCDEIVEQMDFSFLFDVERKLFTIGYNVTASRADESYYDLLASEARLASF
                     VGIAKGDVPQQHWFRMGRALTKVDGGRALISWTGTMFEYVMPLLVMRNYPMTLLAETY
                     KTIVARQIEYGNERGVPWGISEAAYNVRDLHFNYQYGPFGVPGLGLKRGLIEDLVVAP
                     YATMLAAGIDPDAAMDNLRRLQKEGALGPYGFYESIDYTAERLPEGQKSVIIRAFMTH
                     HQGMSLVSLANILHEDLGAKRFHSDPTVQATELLLQERIPVGVPAAHPRSEEVLTGRV
                     AQAMPGMITRVYESADLDTPRTQLLSNGSYNVMLTTAGSGYSQCEDNAVTRWREDVTC
                     DNWGAFIYLRDVRSGAVWSAGMQPTQRKPQSYHVAFSEDKADFRRVDSGISTRMEVVV
                     SAEDNAEVRRISLTNHGSRTREIELTSYAEIVLASQNADAAHQAFSNLFIETEFVPGE
                     NAIMAHRRQRSSDERPIWGVHVVVAEGEQVGAVQYETDRGRFLGRGRTAGNPIAVMED
                     RPLSNTTGAVLDPVFSLRRRVRIPPNQTVRCSFSTAVARSREEALALADKYHDPNIFE
                     RELRLAWTKAQVEMSHLKIDAEEAHLFQRLAARIVYSDPSLRPTPHVLALNTKAQSSL
                     WAYGISGDLPIIVVRINNGADMRTVKKLVRGHEYLHYKGLRVDLVILNDTPTDYLQLL
                     HGDLDTTIRTSGLQALQDKPGGVYLRRSDQMPEADRILLHAVARVVIVAERGSFEDQI
                     ERRRVEEPLPPPLVPRVPSQTYPELTAPQPDLTFFNGLGGFHQGGREYVTLLGAEQWT
                     PAPWSNVIGNNVDFGFLITETGSGCTWSVNSRENRLTPWSNDVVSDPPGEVVYLRDED
                     SGSVWSATPLPIRDREPYIVRHGQGYSVFEHTSHGISQELLVFAPLDAPVKISLLRLR
                     NRTARKRRITVTNYNELVLGVSRASSAPYIITEIDANNSTIFAKNPFNNEFAERVAFA
                     ATNERMLSATCDRKEFIGRNRSLETPAALRRLNLAGRDGAGLDPCAAIQTSVELAPNE
                     AREVIFLLGEAESKAAAQSVVSAFRQPGNVNAAFERVLAHWDKLLGTIEVRTPDAALD
                     TMLNRWLLYQSLSCRVWARTAFYQSGGAFGFRDQLQDVMALVYSSPDITRKQIVMAAQ
                     HQFKEGDVQHWWHPPSDRGVRTRISDDLLWLPFVTSFYINVTGDESVLQEVVPFLEQP
                     PLAPDQHEIYMQPTVSQETAPIYEHCARAIDRSLAVGKHGLPLMGAGDWNDGMNRVGI
                     GGEGESVWLGWFLYTTLATFTPYVDARNEKARGDRYRKHLDTLRKSLEEKAWDGDWYR
                     RAYFDDGTPLGSARNEECRIDSIAQSWAVISGASDPYRMGRAMAAVEEYLIRRGDGLV
                     ILFTPPFDKGNLDPGYIKGYVPGVRENGGQYTHAAIWTMIAYSMLGDGERAGELFSLL
                     NPINHSSTRAGLHKYKVEPYVAVGDVYAVPPHTGRGGWTWYTGSAGWMYRAGLESMLG
                     FKLQKDRLEVDPCVPRWWREFEIVYRRGRATYRIKVENPLGVSRGVVSVEVDGVAQNV
                     IELIDDEKMHTVRIVMGEQVKTEETSEPAQQAQA"
     gene            9998..10492
                     /gene="ruvC"
                     /locus_tag="VFH96_00615"
     CDS             9998..10492
                     /gene="ruvC"
                     /locus_tag="VFH96_00615"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET6972488.1"
                     /translation="MRVLGIDPGSQTTGWGVVEGDGRRYSLVAFGSIRASSSLRFSTR
                     LLKMCTGIEEVIAQHRPDACSLEDAFLATNVKVSMKLGQVRGVVLLVAERAGLDIHEY
                     SPRLIKQTVVGYGNAEKHQVQEMVRLLLALKSIPTPHDAADALAVAICHFHHAAFKER
                     IAAV"
     gene            10528..11241
                     /locus_tag="VFH96_00620"
     CDS             10528..11241
                     /locus_tag="VFH96_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972489.1"
                     /translation="MIPHKLTLCLLFILFLQITGFAQKKPPGGGRLAIVVDERLSPLR
                     ATPQLNGKLVRRLSRGRLVAIRSSKTNADGITFLLVNVTRRTHGWIQREAVVSPSRSG
                     DDRRLLTLIQRSEGFDRIARARIFLDNFPRSPLRPEVLLLLGDTAEELAAKLTIDAAR
                     RLKDDLGDAPAFSYFLNYTGLDRYNRQRVGFVFDKTTKRFHYDGAAWRELIRVYPRSS
                     AASEARKRLDLLFSQERIM"
     gene            11252..11662
                     /locus_tag="VFH96_00625"
     CDS             11252..11662
                     /locus_tag="VFH96_00625"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HET6972490.1"
                     /translation="MTLKLKPKPLGECKMGLTHVAVRLFNSASSDTYEADFLVDTGAT
                     DTMAPASDLKKLGIQPLGKDVYELANGELVEYEYGNAELRFMNEIVPIRIVFGPDGSE
                     PILGVIALEAAGFIVDPKNRMLRKLRARSLKRVA"
     gene            11781..12344
                     /locus_tag="VFH96_00630"
     CDS             11781..12344
                     /locus_tag="VFH96_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6972491.1"
                     /translation="MQAAVKPLRLVINEPASELENLFQAHHGRVFRTAQRITGSAADA
                     EDVLQTIFLRLVKGQDDYDWSRNPEAYLSRAAINASLDLLRSRTRSKSVSLDESEVDS
                     IAGGYESPEKVHADRELQSLVREAVSRLGKTAGEMFVLRYYEGFDNKDIATIMKTSPL
                     VVGVVLHRARTRLRKEIGHYLEKHHEE"
     gene            12334..15012
                     /locus_tag="VFH96_00635"
     CDS             12334..15012
                     /locus_tag="VFH96_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET6972492.1"
                     /translation="MKSNKEDQILDKVTAEIRNEKVDPRAVASAADRVWARVSNAAGA
                     TEFQLPTVDRIEGCADFQSLIPAHLAGKLSEARSLLLVDHTHECIPCRKAMNEARAKR
                     SVTIKPATRNKRYSMQPAVMRWAIAAAVVIGIGLLAVPFVQRFWPYGQFDATVQAAEG
                     QVYQVADTRTAAVATGAKLQQNEKVRTAKDGRAVVRLGDGSLIEMKDRSELYLTKAGK
                     NTTIHLSRGNIVVEAAKQKDGQLFVESGDSLVSVTGTIFSVNNGTKGSRISVIEGEVN
                     LTHAGSDHVLRPGDQATTNPSITTIPVKDEVAWSRNAAKYAAVLNGLANVSNALKSVP
                     QPGVRNSTHLLDLMPENTVVYAALPNFANTIVESHRVIQERISQNAALREWWEKEQAG
                     RAQNMDQVVETIRQFGSYLGDEIAVSVSMDNQGRPTEPLVLAELKNSQGFRQFLEQEI
                     AKYAGDKKGRPEIVFVENPMSAVSQSDDKHERLYVWIQENLLVASPKLEQLQGVGIAI
                     GNGNATSFTATPFRNRIAEIYQTGAGLVVAANLEKVVAATKAERSKGPNAEKQENALN
                     KLGILSVKYFVLDQKDTNGKTHTEASLSFNDANRGIPSWLAAPGPMGSLEYISPDANV
                     VAGFVVKNPATLVDELLGVLETVNPELRKNLDKLQSEHGLNIRDDIAAPLGGEFAFAI
                     DGPILPTPSWKIVFEVNDPAHLQQTLERVVADVNKQAAYFGKSGLTWEKADVGGRTYY
                     TLRSVDFGALQVNYVYANGYLILGPSRALVERALTSQENGLSLLRSAKFTAGLPADGN
                     ANFSAVFYHNIGGLVPAGVATAAQNLPSGPQQAIKSIASDMPPTLAYAYAQGDTITFM
                     ANTEGGPFGIGPATLLGMPNALEMQHIIGRGMGSNK"
     gene            15106..15477
                     /locus_tag="VFH96_00640"
     CDS             15106..15477
                     /locus_tag="VFH96_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972493.1"
                     /translation="MKVVLISLVSWLCGLLILSTFYVGDGKEISVTVTSMIMAALLFS
                     LAYAPSLFWLRQRMGGCKPALLFPLASAVVLNTPVFLIEFLAIGRTLAPSDALACIVA
                     FLLMGTVFGLGFVWNYQDAAL"
     gene            15487..16473
                     /locus_tag="VFH96_00645"
     CDS             15487..16473
                     /locus_tag="VFH96_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972494.1"
                     /translation="MALPASVPEEPIRSQAALTEPPVIDLHGLSVTFGHRPILKNLRG
                     DLRGKAIGLLGPNGAGKTTLIHTLLGFHAPSAGTAQIFGMDIIDQTKEIRSLIGYMPE
                     RDSFIAKMSAVHFVRLMGELSGLPSEAALERAHEALFYVGLGEARYRRLETYSLGMKQ
                     LAKLAQAIVHGPRLIFLDEPTNGLDPPARLRMIKLIREIRDSGHAHIVLSSHLLLDVE
                     ECCDEILILKDGEIAVYCNLEEERKSNRKFLMLETRGDQAKFVQAIQQLGCEFAVSGD
                     HRLKIVLQEGVEVRDLYRLAEEQQVQLRRLSYKRDSLEDIFLKAMENGYGGL"
     gene            16460..17380
                     /locus_tag="VFH96_00650"
     CDS             16460..17380
                     /locus_tag="VFH96_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972495.1"
                     /translation="MAVYEQTYKPYKGKLTPEWSRFLILPRHTLRGVFNSKLFTAFYV
                     ICFIPLLVEAVLIYLHHNVTALAIMNLNARQLIPIDGSFFQTFVVVQGALAFIVALLV
                     GPPLVSRDLRNNALPLYLCRPFSRTEYVMGKMSVLLILLSWITWVPQLLMFLFQSYLE
                     GFAWLRSNLWIASSIIISGLVWILLLALLSQTISALVKWRIVASGVMLGMVIIPSAFA
                     GIVNVLFQTRWANIITLSGLMYNVTAGLFGTFDRVSGRIQISDFDDNVVREVLLLEPP
                     LWCSWGALFIFSAVCLAILHWKVKAYEVVR"
     gene            17415..18359
                     /locus_tag="VFH96_00655"
     CDS             17415..18359
                     /locus_tag="VFH96_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972496.1"
                     /translation="MSTQLIIFDNVSKFYGEILGVNRVNLQIAPGITSLVGPNGAGKS
                     TLMNLMTGLLRPTRGSIKLLGIPTDEPERLFRKVGYCTQFDSFPRGATGREFITSFLL
                     VNGFDRKQANDLAQLAIERVNLVEAADRKVAGYSKGMRQRIRLAQSIAHQPAVLILDE
                     PLNGLDPMVRAETIALFRKLAAEGLHLIISSHILHEVDMMSDRVVLLNNGYIVAEGNI
                     HGVRDEMEEHPMQILIRCDQPAKLAAYVFAQNHVVEARLHDDRRGLFVKTRDADRFYL
                     LLNHAVAEGQVSVESVAPVDDDLSAVYQYLIGTTGGSA"
     gene            18371..>18994
                     /locus_tag="VFH96_00660"
     CDS             18371..>18994
                     /locus_tag="VFH96_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972497.1"
                     /translation="MSSAVELAQATSEQMPSRWGLWLRQIGAIFRLEIEKNFLSRRSI
                     LIYLIAFSPLIPLLLLAPFTPPGKEWRDFNQYAVIYSIIYNSLLVRTIVFFGSAWIFM
                     NLFRGEMVDRSLHYYFLSAVRREVLVVGKYLSGLVTSIVLFTTTTVIAMLLLYFPHFY
                     SQSARYFTEGRGLTQLLTYAGITILACVGYGAFFLVVGLFIRNPIIPA"
BASE COUNT         4061 a         5202 c         5542 g         4189 t
ORIGIN      
        1 ccagccggga gtcgacattt ttgcgtgcac gcacgttctc gcccaccttg gtctcggtcg
       61 cgccctcgct atcaacggcg acaccgcggg tgcgcgcaag gcgtaccagg atttctttgc
      121 actgtggaaa gatgccgatc acgacttgcc ggtgttggtc gacgcgcgca aagagtacga
      181 gacgctaaag tagattcttt ttatccacag attgcacaga tggcacagat gattaataga
      241 atctgtgtaa tctgtggatg agaagctgtg ttgtgatata tttcccgtcc gacccacaaa
      301 ctccagaagg atctgaaaca caatgtccgg acattcgaaa tggcacagta ttaagcacaa
      361 gaaaggcgcg ctcgatgcca agcgcggcaa actgttcacg aagtttatta aggaaatcac
      421 tgtcgcggcg cgcaccggcg gcggtgaccc tgacgccaat gcgcgtctgc gcaaagcgat
      481 tctcgacgcg aaggccggca acatgcccaa cgagacgatc gatcgcgcga ttcgccgcgg
      541 cacgggcgag gaagaaggcg tcaattacga agagatcaca tacgaaggtt atggtccggg
      601 cggcgtggcg ctgctcatcc agtcgatgac tgacaatcgc aatcgcacgg tcgcggagat
      661 ccggcacctg ttttcgaaga acggtggcaa cctcggtgag agtggctcag tgggttggat
      721 atttgagaag aagggttaca tcgttgtcag caagtctgac aaatcagagg aagagctttt
      781 cgatctcgtg attgaagccg gcgcagacga tctgcgcgat gatgtagaca atttcgagat
      841 catcacgtca ccggaagcgt tcgaaggagt gcaggcggcg gtgaaaggcg caggcatcga
      901 gccccaggtc gcggaagttg agtttgtacc gcaaaactat ataaaactcg aaggctcgga
      961 cgcgcgccag atgttgaaac tgatggaagc gcttgaagac catgacgacg tgcagaaggt
     1021 ctcagcgaac ttcgacatca gtgaagcaga catggcagct gtgtaaatcc tggaaaatct
     1081 tgtaaatcct gtagaagtct tagacaagat ttcaggattt tacaggattt acaagatcag
     1141 gcctgagcct gctgcgctgg ttcgcttgtt tcttcggttt tgacctgctc gcccattacg
     1201 atccgaacag tgtgcatctt ttcgtcgtcg attagctcga tgacgttctg cgccacacca
     1261 tcgacctcga cgctcactac accgcgactc acacccaatg ggttctccac tttgatccgg
     1321 taagtcgcgc gtccgcggcg gtaaacgatc tcgaactcgc gccaccagcg cggcacgcag
     1381 ggatcgacct cgagtcgatc cttttgcaac ttgaagccca gcatcgactc cagtcccgcg
     1441 cgatacatcc aacccgccga gcccgtgtac cacgtccacc cgccacgacc agtgtgcggc
     1501 ggtaccgcgt aaacatcacc gactgcaacg tacggctcga ccttgtactt gtgcaggcct
     1561 gcacgagtcg aggagtgatt gatcggattc aataacgaga acaactcacc ggcgcgctca
     1621 ccatcgccga gcatcgaata cgcgatcatg gtccagatcg ccgcgtgcgt gtattgcccg
     1681 ccgttctcgc gtacacccgg aacgtatcct tttatataac ccggatcgag attgcctttg
     1741 tcgaacggcg gtgtgaagag aatcacgagt ccatcgccgc gccggatcag gtattcctcg
     1801 actgcagcca tcgcccggcc cattcgatac ggatccgacg ctcccgaaat cacagcccac
     1861 gattgcgcga tcgaatcgat gcgacactct tcattgcgcg ccgaaccgag cggcgtaccg
     1921 tcgtcgaaat aggcgcggcg gtaccaatcg ccgtcccacg ccttttcctc gagcgacttg
     1981 cgcaaagtat cgaggtgttt gcgatagcga tcgccgcgcg ccttctcgtt gcgtgcatcg
     2041 acatacggtg tgaaagtagc gagggtcgtg tagagaaacc aaccgagcca gacgctctca
     2101 ccctcgccac cgatcccaac gcggttcatg ccgtcgttcc aatcgcccgc gcccatcaac
     2161 ggcagtccgt gcttacccac cgcgaggcta cgatcgatcg cacgcgcaca atgctcgtaa
     2221 atcggagctg tttcctgcga gacggtcggt tgcatgtaga tctcgtgctg atcgggagcg
     2281 agcggcggct gttcgaggaa tggcacaacc tcctgaagta cggattcgtc tccggtaaca
     2341 ttgatgtaga acgaagtcac aaaaggcagc cacaacagat cgtcggagat gcgcgtgcgc
     2401 acgccgcgat cgctcggagg atgccaccag tgttgcacgt cgccttcttt gaactggtgt
     2461 tgggcggcca taacaatttg tttgcgcgtg atgtcaggac tgctgtagac gagcgccatc
     2521 acgtcctgta actgatcgcg gaacccgaaa gcgccgcccg attgataaaa agccgtgcgc
     2581 gcccaaacgc gacatgagag cgactggtac agcagccagc ggttgagcat cgtgtccagc
     2641 gctgcgtcgg gcgtgcgcac ttcgatcgtt ccgagcagct tgtcccagtg cgcgagcacg
     2701 cgttcaaacg ctgcgttaac gtttccgggc tgacgaaaag cggagacaac ggattgcgct
     2761 gccgctttcg actctgcttc gccgagcagg aagatgactt cacgcgcttc gtttggtgcg
     2821 agctcgacgc tcgtttggat cgcggcgcac ggatcgaggc cggcgccgtc tcttccggcc
     2881 agattcaagc gtcgcagcgc ggcaggcgtt tcgagcgagc ggttgcgtcc gataaactcc
     2941 ttccgatcgc acgtcgcgct caacatcctt tcgtttgtcg ccgcgaaggc aacacgctcc
     3001 gcgaactcgt tgttgaaagg gttcttcgca aagatggtcg agttgttcgc gtcgatctcg
     3061 gtgatgatgt aaggcgcgga cgaagctcgc gacacgccga gcaccagctc gttgtagtta
     3121 gtgactgtga ttcgtcgttt tcttgccgtg cgattccgca aacgcaacaa cgagatcttc
     3181 accggggcat cgagcggtgc gaagacgagt agctcctgcg aaatcccgtg gctcgtgtgt
     3241 tcgaagacgc tgtaaccctg cccgtgcctg acgatgtacg gctcgcgatc gcggatcggc
     3301 agaggcgtcg cggaccagac actgcccgag tcttcgtcac gcaggtagac gacttcaccc
     3361 ggcggatcgc tgactacgtc gttggaccat ggcgtgagac gattctcgcg actgttcacc
     3421 gaccacgtgc agccgctgcc agtctcggta atcaggaacc cgaaatcgac gttgttgccg
     3481 atgacattgg accacggcgc cggcgtccac tgttccgcgc cgagaagggt gacatattca
     3541 cggccgccct ggtggaaacc gccgaggccg ttgaagaacg tcaggtcggg ctgcggcgcc
     3601 gtcagctccg gatacgtttg tgacggaact cgcggcacaa gcggcggtgg cagtggctct
     3661 tcaactcgcc gacgctcgat ctgatcttcg aacgacccgc gctccgcgac gatcacgacg
     3721 cgcgccactg cgtgaagcag aatgcgatcg gcctcaggca tttgatccga gcgccgtaag
     3781 taaactccgc cgggtttgtc ctgtaacgct tgcaagccgc tcgtgcgaat cgtcgtgtcg
     3841 agatccccgt gcagtagctg gagatagtcg gtcggcgtgt cgttgagtat cacgagatcg
     3901 actcgcaatc ctttgtaatg aaggtattcg tgaccgcgta cgagcttctt cacggtccgc
     3961 atgtccgcac cgttattgat gcgcacgacg atgatcggca ggtcgccact gatgccgtaa
     4021 gcccacaggc tcgactgcgc tttcgtattg agcgcgagca cgtgcggcgt cgggcgcaac
     4081 gacggatccg agtagacgat gcgcgctgcg agacgttgaa acaggtgcgc ttcttccgca
     4141 tcgatcttca ggtggctcat ttcgacctgg gccttggtcc aggcgagacg aagctcgcgc
     4201 tcgaagatgt ttggatcgtg atacttgtcc gcgagcgcga gggcctcttc acgcgaacgt
     4261 gcaacagcgg tgctgaacga gcaacgaacc gtttgattcg gcggaattct cacacgccgt
     4321 cgcaggctga aaacgggatc gagcacggcg ccggtcgtgt ttgaaagcgg gcgatcctcc
     4381 atcacggcga tcggatttcc ggcggtgcgc ccgcgaccga gaaaacgtcc gcgatcggtt
     4441 tcgtattgga ccgcgcccac ctgctcgcct tccgcaacga cgacgtgcac gccccagatc
     4501 ggacgctcgt ctgaagagcg ttgccggcga tgggccatga tcgcattctc gccgggcacg
     4561 aactccgtct cgatgaatag attgctgaac gcctggtgtg ctgcgtcggc gttctggctg
     4621 gccaaaacga tctcggcgta actggtcaac tcgatctcgc gcgtgcgtga accgtgattt
     4681 gtaagcgaaa tgcgacgcac ttccgcattg tcttctgctg aaaccacgac ttccatgcgc
     4741 gtcgagattc ccgaatcgac gcgacggaag tcggctttgt cttccgaaaa cgcgacgtga
     4801 tacgactgcg gtttgcgttg tgtcggttgc ataccggcag accacacagc gccgctgcgc
     4861 acgtcgcgca ggtaaataaa cgcaccccag ttatcgcagg ttacatcttc acgccagcgc
     4921 gtgactgcgt tgtcttcgca ctgtgaatag cccgaacccg cggtggtgag catgacgttg
     4981 taactgccgt tcgaaagcaa ctgggtgcgg ggcgtgtcga gatccgcgga ttcgtaaacg
     5041 cgcgtgatca tgcccggcat ggcttgcgcc actcgtccgg tcagcacctc ttcggagcgc
     5101 ggatgagccg ccggcacgcc gacaggtatt cgctcctgca gcaacaactc ggtcgcttgc
     5161 accgttggat cggagtggaa acgtttcgcg cccagatctt cgtggaggat gttcgcgagc
     5221 gataccagac tcatgccctg atggtgcgtc atgaacgcgc ggatgatcac tgatttctgg
     5281 ccttccggca gtcgctcagc cgtatagtcg atcgactcgt aaaagccgta tggcccaagt
     5341 gcgccttctt tctggagccg gcgcaggttg tccattgcgg catcaggatc gattccggca
     5401 gccagcattg tcgcgtatgg agcgacgacg agatcctcga tcagcccgcg tttgagaccg
     5461 agaccgggaa caccgaaggg gccatattga taattgaaat gcagatcgcg cacgttgtag
     5521 gccgcttccg agattcccca cggcacccca cgctcgttac cgtattcgat ctgtcgcgca
     5581 actattgttt tgtaagtttc tgcgagcaaa gtcatcgggt agttgcgcat gacgagaagc
     5641 ggcatcacat actcgaacat cgtaccggtc catgagatca acgcgcgtcc gccgtctact
     5701 ttcgtgagcg cacggcccat acggaaccag tgttgttgcg gcacatcgcc tttagcgatg
     5761 ccgacaaagc tcgcgagacg tgcttccgag gcgagcagat cgtagtaaga ctcatcggcg
     5821 cgggaggcgg taacgttgta accgatagtg aagagtttgc gctccacgtc gaagagaaac
     5881 gagaagtcca tttgctcgac gatctcatcg caacggcgcg cgagacgact cagacgcgac
     5941 aacatgtctg acgaagcgcc ggcggattgc tcgagcgcct tggtgagttt tgaagcgtcc
     6001 gctgaggcat ctgcatgtgt agccgcaagt tggaccagag ctttatcgca aagctgtggt
     6061 atttcggcga gtgccggcac ctgctgtagg agtttgatcc actcggcgct gtttatttcg
     6121 ttttcgaccg aagacagcaa gcgaccccag cctgtcagcg attccgcatc tcggcgcaat
     6181 gatttgacct ggtgttggag tgcgccaacc caccagcgca gctccttgaa gctgacctcg
     6241 ccatgctcgt gaacgagagc gtttacgatg tcttcgattt cagaggcacg gcgtccgagt
     6301 gaatcaaaga gagcgatcca tgacgataga ctctcggcgg gatcgacttg caacaacgcc
     6361 cgacacgcgg cgatctcgtc gtgcaactgt ctcaccgtca cgacgtcagt gcgttgccgg
     6421 aagctgccca acgcactcgc ttcggtgtcg atggcgttga tcgtgtcggt cagtccttca
     6481 atcacgtgca catcaaaaag acgcgtttcc ggaaactcga tacaggcttg tttgactgcg
     6541 attaagtgtc cagcaaggtt gccgctatcc accgtcgaga tgtattgcgg caggagtggt
     6601 tcgagagttt tggtgtcgta ccagttaaag tagtgaccat gcattcggcc gagtttctgt
     6661 aacgtcgcaa aggtgagctc ctgtcgctcg agtaattcga gcgcgccgag atagccgaga
     6721 tctcgtgccg agtggttgcc gagtagcaac aagcccatgt ttgtcggtga cgtgcgatgc
     6781 gcgacgacgg gtgacggatc ttcctggtag ttgtcgggtg gcagccagtt atcttcaggg
     6841 ccgacgaaag tttcaaagaa gcgccacgtg cgacgtgcga tcagtcgtgc gaacgctttg
     6901 tcttcttcgt tcaaaagctt tcgttctgcc ggtcgcggct tgctcacgaa gtaggcgatc
     6961 aatggcgaaa gaatccagat cacgaagagt gtgctgatct ccggcagggc gcgaggttta
     7021 aaactcaacg tgagtccgag cgcggctacc gttagtatca acgccggcaa catgaagcga
     7081 acgaacgact gcagattgtt tcgcaaagag ctttccgctt cggctgccga cacccattcg
     7141 agcaacttcc tgcgcgagat taactggcga tagacggcac gcacgatcgc gtcgcacatc
     7201 aaccatgcct ggtgtgggag aaacacaaac gacaacgcga tctgtgcggt gttggtgcgg
     7261 aagtcgcccc agcggctcca gaaatggctg gtccacggaa tgccgcgcgg gtgtattgcg
     7321 aggcccgtcg tcacgtgcag gtaaactgga aacgcaatga cgagaaaaac aaagagggac
     7381 caaagcaacg ccgagccggg aaataacgcg cacgaagcca cgagccacag aaacaacgcc
     7441 ggtgcgacaa ggctgcgacg aagattgtcg agaatcttcc agcgtgagat cagcggcagc
     7501 gtgttgtgga cttttttgcg attgccgtcc ggaacccccg gaaacaacca gcgactgatc
     7561 tgccaatcac cacgaaccca gcgatgcgac cgcttggcgt aagcttcgta cgacgcggga
     7621 tagtcgtcga gcaattcgat gtctgtggtg agcgcggcgc gcgcaaataa actctcgaag
     7681 agatcgtgac tcagaaggga attcggcggc acgcgattct cgagcgtcgt ttgaaacgca
     7741 tcgacgtcgt ataagccttt gcccgtgaag ttaccttcac cgaagaagtc ctggtaaacg
     7801 tctgacactg ccgtcgtata aggatcgatg ccggtgtagc cggagaagac ctgaacaaat
     7861 ttcgagcgtg atgcgcttgc gagagcgatg ctgacgcgtg gctgcaaaat gccgtagccg
     7921 cgggtgacgc gattcgtcgc ttcgtcgatc tttggttgat tgagcgggtg gatcgcggcg
     7981 ccgacgagct tgcgtgcaac gtcacgcgga agcgacgtgt cggagtcgag cgtgatcacg
     8041 taacggatcg atttaagcag ggcgtcgttg gcagtgcgca cgacgaagct cgtgtcctgc
     8101 gcgccgcgca gcagccggtt gaactcttcc agcttgccgc gtttgcgttc ccagcccatc
     8161 catttgtgct cgcctgcgtt ccactgtcgc tttctgtgga agaggtgaaa gcgctcgttg
     8221 ccgtggttgc gattcagggc gtcgataccg ctttgcgcga tcgcgaggag acttgaatca
     8281 gccggtgttt gttctgcgtc ggcgtcgggg aagtcgctca gcaatgcgaa atagatctgt
     8341 tcgtcctggt ttgccagata gtggacctcg agtcgctcga ccagttcgtg cacctgagaa
     8401 tcgttcagaa agatggtggg cactacgacg actgtcgccg cgtcgtcggg aatgccgtcc
     8461 gacgtgttca tcctcggcag cagacgtggc ggaaagaagt gcgtgagatc ccagttgagc
     8521 accgtgatcg cgagatcgct cgcagggatg agcgccagca acgccgtcaa gattagaaac
     8581 ggccaaccga ctctgtatcg agacatgaaa taaagcagcg gcgtcatgat caggaacgtc
     8641 atgacggtca gtgtgcccag atacgtggta gtgcagtggc gcagcaggaa acggcgaagt
     8701 cgctcggtag tgcccggttg ataacgaaac ttcgtctcga gttggtttaa gccggcgtcg
     8761 atcaggtagt aaccgacgtg tcgttgcgcg cgcgatcttt cgttcgcgtg ggcggcgaga
     8821 tcgatcgcgg cctggccgat atcgagctcg tttgtgcgcg ttcgtttgct gatgcgctca
     8881 atcacgtgtc gatagcgatc gcgcgctgca aactccatct gcgcgtaaac gccggcggga
     8941 tcgttgccca gcagccgctc gatcagactg actgtttcaa agaagtcgtt ccagtcgagg
     9001 gtcgagagca ggcgcatgct ggtgatgatg tttccgacag tgacttgcgc cgcggcttgt
     9061 cgctggtgtt ccgagtgaat gatctgttcg accgtcgtgc cctggtttgc gacctctttc
     9121 tcgatccagt ccatcacggg catcacggac gggtgttgtt cgcgcaggcg ttgcacgagt
     9181 tggaccagaa atgcgtgtgg caggtcttcc cgttttccga gacgatcgtt cagggacgac
     9241 atgacgttcg ccggttctag tgatgcggtc tctagcagtt tgtcggcgag cttgtcggct
     9301 tcgtcgcgct cgaggcgggc gcgagcgatg gcgattgcga gacggcgcag gttttcgacg
     9361 agtgctagtc gcagcgtgat agcgacggcc cacagctcgc cgatcgagag tggcgcaaca
     9421 gtttgatatg acgcgataaa tctttgtagc gtgttcgtgt cgaggcgcga gtcagtgtgg
     9481 gcgatcaggg cgagtgcgac ggcgtaaatt cttggatagt ccgctagttc gccttcagcg
     9541 agtttgggaa gctcgtggta gtagctcgtg ggcaagtcct gccggatctc gcgcagttgc
     9601 tcttcgatta tgtggtagtt gtctaccagc cattcggcgg ctggcgagat cgcgcggcct
     9661 tcgcgcaggg cgttgacgag agctttgtat gcggcttcga gtttgcggcc gttgtcttcg
     9721 aggcgcggca ggagttgtgc gcggcccttg cgcgttaccg ttttgtgttc ggcggcgaga
     9781 gtttgtgcgt attgttcgag gcgctcgaca ctgaagaact cagcctggaa aggcttctcg
     9841 tcagcgtagc ccgtaggttt acctaaaaat gaagacactg attcgatgaa ctcactcata
     9901 gagtgtgccc gagttacaca caagcagggg gactaaaggg gtttgaggat aacattttgt
     9961 cgttgtagtt ggtaacggcg ttgtatactc gcgcgcgatg cgcgttttag gaatcgatcc
    10021 gggaagccag acaacagggt ggggcgttgt cgaaggcgac ggacggcgtt actcactcgt
    10081 cgcgtttggt tcgattcgtg catcgtccag cctgaggttt tccacgcgct tgctgaagat
    10141 gtgtacaggg atcgaggaag tgatcgcgca gcatcgtccg gatgcgtgct cgctcgaaga
    10201 tgcatttctt gcgacgaacg tgaaagtcag catgaagctc gggcaggtgc gcggtgtggt
    10261 gctgctggtt gcggagcgcg cggggctgga tattcacgag tattcaccgc ggttgatcaa
    10321 gcagacggtg gtgggttacg ggaatgcaga gaagcatcag gtgcaggaga tggtgcgatt
    10381 gctgttggcg ctgaagagca ttcccacgcc gcatgacgcg gctgatgcgc tcgcggttgc
    10441 gatttgtcac ttccatcatg cggcatttaa agagagaatc gcggcggttt aagacgtaat
    10501 ttcaacctca cgattggaga acaaaacatg attcctcaca agctcacgct ttgtctgctt
    10561 ttcattctct ttcttcagat aaccggtttc gcgcagaaga aaccgccagg cggagggcga
    10621 ttggccatcg tcgtcgacga gcgcctgtcg ccacttcgcg caactccgca actcaacgga
    10681 aagctcgtgc ggcgtctcag ccgtggccga ctcgtcgcca tccgatcctc gaagacaaac
    10741 gccgacggca taacttttct tctcgtgaac gtgacgcgtc gcacgcatgg ctggattcaa
    10801 cgcgaggcag tggtttcacc ctcgcgctcc ggtgacgatc gaagattatt aactctgatc
    10861 caacgttcag aaggcttcga tcgcatcgcg cgcgcgcgaa tttttctcga caacttccca
    10921 cgttcaccgt tgcgtccgga agttctatta ctactcggcg acaccgcaga agagcttgcc
    10981 gcaaaactaa caatcgacgc cgcacgccgt ctcaaagacg acctcggcga cgcacccgcg
    11041 ttcagttact tcctgaacta caccggtctc gatcgctaca accgtcagcg cgtcggattt
    11101 gtcttcgaca agacgacgaa gcgctttcac tacgacggcg ccgcctggcg cgaactaatc
    11161 cgcgtttatc cgcgttcatc cgcggctagc gaagcgagaa aacgcctgga cctacttttc
    11221 tcccaagaga gaataatgta gtatccgcac catgactctc aaactcaaac caaaacctct
    11281 aggagaatgc aaaatgggcc tcactcatgt tgctgttaga ctattcaact cagcttcgtc
    11341 ggatacatac gaggcagatt tcctcgttga tacgggcgcg acggatacaa tggctccggc
    11401 atccgatctc aaaaaattag ggattcaacc actgggcaag gatgtttatg agctggccaa
    11461 cggtgaactt gtcgaatatg agtacggtaa tgccgaacta cgattcatga atgaaatcgt
    11521 ccctattcgt atagttttcg gccctgacgg cagtgagccg attttaggcg taatagcttt
    11581 ggaagccgcc gggtttatcg tagatccgaa gaaccgaatg ctccggaagc tccgagcgcg
    11641 ttcgctaaag cgggtagcct aagcaaaaaa ctatcgccgg cggggcgatc cacactaaac
    11701 ttccaaaaat tcgtgctggc gggtgttaat aacgccttaa cccactcgtc tatcagggtg
    11761 tagacgaagg agaaacaccc ttgcaggcag cagttaaacc cttgcgatta gtgataaacg
    11821 aacccgccag cgagctggaa aacctcttcc aggcacatca cggccgagtt tttcggaccg
    11881 cacagcggat tacgggcagc gccgccgacg ccgaagacgt gctccagacg atcttcctcc
    11941 gccttgttaa aggccaggac gactacgact ggtcgcgaaa tcctgaggct tatctttcgc
    12001 gcgccgccat caacgcctcg ctcgatttac tgaggagtcg cacccgctcg aagtccgtgt
    12061 cgcttgacga aagcgaagtg gattcaattg ccggcggcta tgaaagtccg gagaaggtcc
    12121 acgccgatcg cgagctgcag tcactcgttc gcgaggcggt gtcgcggctc ggaaagactg
    12181 ccggcgagat gtttgtcctg cgttattacg aaggttttga caacaaagat attgcaacga
    12241 ttatgaaaac ttcaccgctg gtggtcggcg tagtcctcca tcgcgctcgc acaagactca
    12301 gaaaagagat tgggcactat ctggagaaac atcatgaaga gtaacaaaga agatcagatt
    12361 ctcgacaaag ttactgccga gatccgtaat gagaaagttg atccccgcgc agtggcatcg
    12421 gcagcagacc gtgtttgggc gcgtgtctcc aatgctgcgg gcgcgactga gtttcagtta
    12481 ccgacagttg accgcattga aggctgtgca gacttccagt cactgattcc ggcacatctc
    12541 gccggtaagc tttccgaagc gcgctcgctg ctgctggtgg accacacgca cgagtgtatc
    12601 ccctgccgca aagcgatgaa cgaagcgcgt gccaaacgtt cggtcacgat caaaccagcc
    12661 actcgcaaca agcgctactc gatgcaacct gccgtcatgc gttgggccat cgcggctgcc
    12721 gtcgtgatcg gtatcggact tttagcagta ccgttcgtgc agcggttctg gccgtacggc
    12781 caattcgacg cgaccgttca ggcggccgag ggtcaggttt accaggttgc cgacacgcgc
    12841 accgccgcgg tcgcaaccgg agcgaagctg cagcaaaacg agaaggtgcg caccgcgaaa
    12901 gacggtcgcg ctgttgttcg tctgggcgac ggctctctga tcgagatgaa agatcgctcg
    12961 gagctctacc tgacgaaagc agggaagaac acaacgattc accttagccg cggtaacatc
    13021 gtcgtcgaag cagcgaagca aaaagacggg cagttgtttg ttgagagtgg cgactcgctg
    13081 gtttccgtca cgggcactat tttctccgta aacaacggca cgaagggctc gcgaatctcc
    13141 gtcattgaag gcgaagtgaa cctgactcac gcaggcagcg atcatgtgct gcgtccgggt
    13201 gatcaagcta ccaccaatcc ctcgatcacg acgattccgg tcaaagatga agtcgcatgg
    13261 agccgcaacg ccgctaaata cgcagcagtg ttgaatggcc tcgcaaacgt cagtaacgcg
    13321 ttgaagagcg ttccgcaacc cggcgttcgt aattccacgc acctgctcga cctgatgccg
    13381 gagaacaccg tggtttatgc cgcactaccg aacttcgcga acacgattgt ggaatcgcat
    13441 cgcgtgattc aggagcgcat cagccagaac gcagcgctgc gcgagtggtg ggagaaggaa
    13501 caagccggcc gcgcgcagaa catggaccag gtggtcgaaa cgattcgcca gtttggttct
    13561 tacctcggcg acgagatcgc agtatcagtc tcgatggata atcagggacg tccgaccgag
    13621 ccgctcgtgc ttgctgaact gaagaactca caggggttcc gccagttcct cgagcaggag
    13681 atcgcgaagt atgcgggcga caagaagggc agaccggaga tcgtgttcgt tgagaacccg
    13741 atgtcagcgg tttcgcagtc tgacgacaaa cacgaaaggc tctacgtttg gattcaggaa
    13801 aacttgctcg ttgccagtcc gaagctggag cagcttcagg gcgttggcat agcgatcgga
    13861 aacggaaacg cgacgagctt tactgccact ccgttccgca accgcatcgc agagatttat
    13921 cagacgggcg cgggtttggt agtcgccgcg aatctcgaaa aagtcgtggc tgctaccaag
    13981 gctgagcgca gcaaaggtcc gaacgctgag aagcaggaaa acgcgctgaa caagctcggc
    14041 atactgagcg tgaagtattt cgttctcgat cagaaagaca ctaacggcaa gacgcacacg
    14101 gaagcgtcac tgtcattcaa cgacgcgaac cgtggtattc cttcgtggct cgcagcgccg
    14161 ggaccgatgg gctcgctcga gtacatctcg cccgacgcga acgtcgtagc cggtttcgtt
    14221 gtgaaaaatc cggcgacact cgtcgacgag ctgctcggcg tgctggaaac ggtaaatccc
    14281 gagttgcgca agaatctcga caagctgcaa tcggagcacg gtctgaatat ccgcgatgat
    14341 atcgctgcac cactgggtgg agagtttgcc ttcgcgattg atggaccaat cctaccgacg
    14401 ccttcgtgga agatagtgtt tgaagtcaac gatccggcgc acctgcagca gacgctcgaa
    14461 cgtgtcgtcg ctgacgtgaa taaacaagcc gcgtacttcg gcaagtcagg attgacctgg
    14521 gaaaaggcgg atgtcggtgg ccgcacgtat tacacactgc gttccgtaga tttcggagcg
    14581 ctccaggtga actacgttta cgcgaacgga tacttgatcc ttggtccaag cagggctctc
    14641 gtcgaaagag ctttgacctc gcaggagaac ggtttgtcgc tgctgcgatc ggcgaagttc
    14701 acggcgggcc tgccggctga tggcaatgca aacttctcgg cagtgttcta tcacaacatc
    14761 ggcgggctcg ttccggctgg tgtggcgacc gccgcgcaga atcttccgag tggaccacaa
    14821 caggcgatca agtcgatcgc gtctgacatg ccgccgacgc ttgcttacgc ttacgcgcaa
    14881 ggcgacacca tcacgttcat ggcgaacact gaaggcggac cattcggaat tggtccggca
    14941 acgctcctgg gtatgcccaa cgcgctcgag atgcaacaca tcatcgggcg gggcatgggt
    15001 tcgaacaagt aggtcttttt aattggccgc agatgaacgc ggatgaacgc agatcagaaa
    15061 tgatcggcgt ttatccgcgt aaatccgcgt taagaaagta ctgcaatgaa ggtcgttctg
    15121 atttcacttg tctcctggtt atgcggtctc ctaatcctct cgactttcta cgtcggtgac
    15181 ggaaaagaaa tcagcgtcac cgttacctca atgatcatgg cagcgctgct gttctcgctc
    15241 gcctatgcgc cgtcgctctt ctggctgcgc cagcgcatgg gtggttgcaa gccggcgctg
    15301 ctctttcctc ttgcttccgc tgtcgtactc aacacgcctg tcttcctgat cgaatttctt
    15361 gcgatcggca gaactttagc accatcggat gcgttagcat gcattgttgc ttttctgctg
    15421 atgggcacag tcttcggact cggtttcgtt tggaattatc aggacgcagc gttataagaa
    15481 gagagtatgg cactccccgc ttcggttcca gaggaaccga tcagatctca agcggctctt
    15541 actgagccac cagtgatcga ccttcatggt ctcagcgtta ctttcggtca ccggccaata
    15601 ctaaagaacc tgcgcggcga tctgcgtggt aaagcgattg gtctgctcgg tccaaacggc
    15661 gccggcaaga cgacgctgat tcacacactc ctcggctttc acgctccgtc cgcaggcacc
    15721 gcgcagatat tcggcatgga catcatcgat cagacgaaag agatccgatc gttgattggc
    15781 tacatgcccg agcgcgactc gttcatagcg aagatgagtg cagttcactt cgtgcgtttg
    15841 atgggtgagc tgtcaggact accttcagaa gccgccctcg agcgtgcgca cgaagcgctg
    15901 ttctatgtag gactcggcga agcgcgttat cgacgactcg agacttattc gctgggcatg
    15961 aagcagctcg cgaaactcgc gcaggcgatc gttcatggtc caagattgat cttcctcgac
    16021 gagccgacaa acggtctcga tccgccggcg cgcctgcgta tgatcaagct gatacgcgag
    16081 attcgcgaca gcggtcacgc gcacatcgta ctttcgtcgc acttgctgct cgacgtcgaa
    16141 gagtgttgtg acgagattct gattctgaaa gacggcgaga tcgccgttta ctgcaatctc
    16201 gaagaagagc gtaaatcgaa tcgcaagttt ctcatgttgg aaacccgcgg cgaccaggca
    16261 aagtttgtgc aggcgattca gcaactcggt tgtgagtttg cagtgtcagg cgatcatcgc
    16321 ttgaagatcg ttttgcagga aggcgtggag gtgcgcgacc tttatcgtct cgccgaagag
    16381 caacaggtgc agctcagacg tttgagttac aagcgtgact cgctcgaaga catctttctg
    16441 aaggcaatgg agaacggcta tggcggttta tgagcagact tataagccgt acaaaggcaa
    16501 actgactccc gaatggagcc gctttttgat tctcccgcgg cacacgttgc gaggtgtctt
    16561 caactcgaag ctcttcacgg cgttctacgt catttgcttc ataccgttgc tcgtcgaagc
    16621 ggttttgatc tatttacatc acaacgtcac tgcgctcgcg atcatgaatc tcaatgcgag
    16681 gcagctgata cctatcgacg gttcgttctt ccagacgttt gtcgtagttc agggagcgtt
    16741 ggcattcatc gtcgcgctcc tggttgggcc accacttgtc tctcgcgatc tgcgaaataa
    16801 cgcgttgcca ctttatctct gccgcccgtt ctcgcgcact gagtacgtga tggggaaaat
    16861 gtcggtgttg ttgatcctgt tgtcctggat cacatgggtg ccgcagttgt tgatgtttct
    16921 gttccaatct tatctggaag ggtttgcatg gctcagaagt aacctgtgga ttgcaagctc
    16981 tattatcata agcggtcttg tttggatctt gctgctggct ttgctttcgc aaactatctc
    17041 agcactggtg aagtggcgca ttgtggcgag tggagtaatg ctggggatgg tgattatccc
    17101 gtccgcgttc gccggtattg tcaatgtact tttccagacg cgctgggcaa acatcattac
    17161 actgtccgga ctcatgtaca acgtaacggc cggtctgttc ggtactttcg atcgtgtcag
    17221 cggccgcatt cagatcagcg acttcgatga caatgttgtc agagaagtac tgctgctgga
    17281 gccgccgctg tggtgctcct ggggcgcact atttattttt tctgcggtct gcctcgcgat
    17341 cttgcattgg aaagttaaag cctacgaggt ggttcgataa tccgtggaga tcggtaaata
    17401 aatccgtgtc atccgtgtct actcagctca tcatcttcga taacgtctca aagttctatg
    17461 gcgagatact cggcgtgaat cgggtcaacc tccagatcgc gcccggcatc acgagcctcg
    17521 tgggaccgaa cggagcaggg aagtcgacgt tgatgaattt gatgacggga ctattgcgac
    17581 caacgcgcgg ctcgatcaag ctactcggta ttcccaccga tgaaccagag agactgtttc
    17641 ggaaagtcgg ttactgtacg cagttcgatt cttttccgag aggcgcgacg gggcgcgagt
    17701 ttattacgtc gttcctgctc gtcaacggtt tcgatcgcaa gcaggcaaac gatctggccc
    17761 agctcgcgat cgaacgcgtt aacctggtcg aggcggcgga tcggaaggtg gcgggttaca
    17821 gcaagggcat gcgacaacgc attcgcctcg cgcagtcgat cgcacaccag ccggcggttc
    17881 tgattctcga cgagccgctc aacggtctcg atccgatggt acgagccgag acgatcgccc
    17941 tgtttcgcaa actcgctgcc gaaggtttgc acctgattat ctcgtcgcac attctgcatg
    18001 aggtcgacat gatgtcagac cgcgttgtgt tgctgaacaa cggttacatc gtcgctgaag
    18061 gcaacattca tggcgtgcgc gacgagatgg aagagcatcc gatgcagatc ctgattcgtt
    18121 gcgatcagcc ggcgaaactc gcggcgtatg tttttgctca aaatcacgtc gtcgaagcgc
    18181 gattgcacga cgaccggcgt ggcttgtttg tgaagacgcg cgacgcggat cgattctacc
    18241 tgttattgaa ccatgctgtt gcggaaggcc aggtgagtgt ggaatcagtg gcgccagttg
    18301 atgacgatct cagcgcggtt taccaatacc tgatcgggac caccggcggc agcgcatagg
    18361 agtttagtga atgagttctg cggttgagtt agctcaagcc acatctgaac aaatgccttc
    18421 gcgttggggg ctctggctcc ggcagatcgg cgcgattttt cgtctcgaga tcgagaagaa
    18481 ctttctcagc cgtcgctcga tactgatcta cttgattgcg ttctcgccgc tcattcccct
    18541 cttgctttta gcccccttta cgccgccggg aaaagagtgg agagatttca atcaatacgc
    18601 cgtcatctat tcgattatct acaacagtct gctcgttcgc acgattgtct tttttgggtc
    18661 ggcgtggatc ttcatgaacc tgtttcgcgg tgagatggtc gaccgcagtt tgcactatta
    18721 ctttttgagt gcggtgagaa gggaagtgct ggtcgtcggc aagtatctct ccggcctggt
    18781 gacttcgatc gttctcttta cgacgacgac cgtgattgcc atgctgctgc tttatttccc
    18841 tcacttttat tcgcagagcg cgcgctactt caccgagggc cgtggtctga cgcagcttct
    18901 gacttacgcc ggcatcacga tactcgcgtg cgtaggttac ggcgcgttct tcctcgtggt
    18961 ggggttgttt attcgcaatc cgatcattcc cgcg
//
LOCUS       DASSFQ010000021         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_12535, whole genome shotgun sequence.
ACCESSION   DASSFQ010000021 DASSFQ010000000
VERSION     DASSFQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12535"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..2005)
                     /locus_tag="VFH96_00665"
     CDS             complement(20..2005)
                     /locus_tag="VFH96_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6972498.1"
                     /translation="MLFRLSEVYKSYGTQDVLRGASLQVNPGEHVGLVGRNGAGKSTI
                     FRLVRGDETADRGDVVRARGVKLGLLDQHVHFKPGSTVHESALAAFGRLQQIEHEMHE
                     LEHRMADAGDDLEKILERYSDLQHEFEHEGGFEYGAKAESILQGLGFDRDTWSMETEK
                     LSGGQQNRLGLACLLLAAPDVLLLDEPTNHLDVNAVEWLEEFLQTYPAAIVIISHDRY
                     FLDRACRRIIELENGRAASYTGNYSDYLVEREERREAQQRAYDNQQQLIAKTQEFIRR
                     NLAGQKTKQAKSRRKMLEKLERIDAVRHDQSSGDFRLGAIERTGNHVLTVNQLSIGYE
                     NNLLARDIAFILRRGECLGIIGPNGSGKTTFLKTILNKLAPLSGEVRWGTKVQIGYYA
                     QQLDDLDDRNEIIMELRRVAPATATAGELRSFLAKFLFTGDDVYKHVQDLSGGEKGRL
                     ALAKLIYSRVNVLVLDEPTNHLDIPSREALEEALSAYEGTIVTISHDRYFLDRVATQI
                     LALDGAGVAEHYDGDYTEYHDWKLGLREAAPAVKMIAEENLVTVAPPVVQPEQPRPAK
                     KPAPAPKKPGVKVVKKKKETRTMELVESDIARAEQRLNEISEQMGTPEVARDPDKLIA
                     LNNEYHQTENLLRNLYEEWDRVTEQSAVAPGLMSDKL"
     gene            complement(2103..>2632)
                     /locus_tag="VFH96_00670"
     CDS             complement(2103..>2632)
                     /locus_tag="VFH96_00670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6972499.1"
                     /translation="ADFATVARAKSEDINSLRAGGDIGFATEQDLKSNNFPESLIASL
                     FGSMQVGDYTQPVQFSGKWYIFKLEEKRLANENLTLESAGVRQQITQGLTNQRKAILN
                     AALLDTAMNEARIVNHLATNMLNNPGNLGLRPAAAGSAPTQAPSQAPAASPAASSPSA
                     TGSGSTSVKPASSPK"
BASE COUNT          514 a          758 c          735 g          625 t
ORIGIN      
        1 gtcgcgacca tacgacaagc taaagcttat cggacatcag acccggcgct actgcgctct
       61 gctctgtaac ccgatcccat tcctcgtaca ggttacgaag taaattttcg gtttggtgat
      121 attcgttgtt gagtgcaatc agcttatcgg gatcgcgagc gacttcaggc gtgcccatct
      181 gctccgagat ttcgttgaga cgttgttcgg cgcgagcaat gtcactctcg acaagctcca
      241 ttgtgcgcgt ttccttcttt tttttgacca ctttcacgcc gggttttttt ggcgcgggcg
      301 cgggtttctt tgccggtcgc ggctgttccg gttgcacaac cggaggagca acagtcacga
      361 gattctcttc tgcgatcatc ttcactgccg gcgccgcttc acgcaaaccg agtttccaat
      421 cgtgatactc agtgtaatca ccatcgtaat gttctgcgac gccagcgccg tcaagcgcaa
      481 gtatctgtgt cgccacccga tcgaggaagt aacggtcgtg actgatcgtg acgatggttc
      541 cttcatacgc tgacaatgct tcttcaagcg cttcacgcga aggaatgtcg agatggttgg
      601 tcggctcgtc gagtacgagc acgttcacac gcgagtagat caacttcgcg agggcgagtc
      661 ttcccttctc gcctccggaa agatcctgca cgtgtttgta gacgtcgtcg ccggtaaaga
      721 gaaacttcgc gagaaaggag cgtagctcac cagcggttgc agtcgcaggc gcaacgcgtc
      781 gcaattccat tatgatttca ttgcgatcgt cgagatcgtc gagctgctgc gcgtagtaac
      841 cgatttgaac cttcgtgccc cagcgcactt ccccactcag cggcgcgagc ttgttcaaga
      901 tcgttttcag gaacgtcgtc ttgccggagc cgttcggtcc gataatgccc aggcattcac
      961 cgcggcgcag tatgaacgcg atgtcgcgcg cgaggagatt gttttcataa ccgatcgaaa
     1021 gctggttgac cgtcagcacg tgattgccgg ttcgttcgat cgcgccgagt cgaaagtctc
     1081 cggacgattg atcgtgacgc acggcgtcga tgcgctcgag tttctcgagc atcttccggc
     1141 gcgatttagc ttgctttgtt ttttgcccgg cgaggttgcg gcgaatgaac tcttgcgtct
     1201 tcgcgatcag ttgctgctgg ttgtcgtaag cacgttgttg cgcttcacga cgctcttcac
     1261 gctcgacgag atagtcagag tagttaccgg tgtagcttgc agcacgcccg ttctcgagct
     1321 cgatgatgcg acgacacgca cgatcgagga agtaacgatc gtggctgata atcacaatcg
     1381 cggctggata ggtttggaga aactcttcca gccactcgac tgcgttcacg tcgagatggt
     1441 tcgtcggctc gtcgagtaac aaaacatcgg gcgcggcgag taagagacag gccagtccga
     1501 gacgattctg ctgaccgccg gaaagcttct ccgtttccat tgaccacgtg tcgcgatcga
     1561 aaccgagacc ttgaaggatc gactcagctt tcgctccgta ttcgaagcct ccctcgtgct
     1621 caaactcgtg ttgcagatcg ctgtagcgct cgaggatctt ttccagatcg tcgccggcgt
     1681 cggccatgcg gtgttccagc tcatgcatct cgtgctcgat ctgttgcaga cgaccaaagg
     1741 cagcgagggc cgattcatgg accgtcgagc cgggcttgaa atggacgtgc tggtccaaca
     1801 ggccgagctt cactccgcgc gcacgcacga cgtcgccgcg atccgctgtc tcatcgcctc
     1861 tgacgagacg gaagatcgtg ctcttgccgg cgccgttgcg gccgacgagt ccgacgtgtt
     1921 caccaggatt cacctgcagc gaggcaccgc gcagcacgtc ctgagtgccg taggatttat
     1981 aaacctctga gagacgaaac aacatatctg actttactta cgcaaagacg aaccacggat
     2041 gacactgatc aaagctgcgg attgatccgt gtcatccgtg gttacggcct tgcccgagaa
     2101 atttacttcg gcgagctggc tggcttcacc gatgtcgagc ccgagccagt ggctgaagga
     2161 ctactggccg caggacttgc cgcaggcgcc tgcgacggag cttgcgttgg tgcactgcct
     2221 gccgcagccg gacgcaatcc cagattcccc ggattgttca gcatgttagt agcgagatgg
     2281 ttaacgatcc tcgcctcgtt catcgccgtg tccaagagag ccgcgttgag aatcgcttta
     2341 cgctggttcg tgagaccctg cgtgatctgc tggcgcacgc ccgcgctctc cagcgtcaga
     2401 ttctcgttgg cgagtcgctt ctcttccagt ttgaagatgt accacttgcc gctgaactgc
     2461 actggctgcg tgtaatcacc aacctgcatc gagccgaaaa gacttgcaat cagactctcc
     2521 gggaagttgt tactcttcag atcctgctcg gtcgcaaaac cgatatcgcc gccggcccgg
     2581 aggctgttga tgtcttcact cttggcgcga gcgaccgtcg cgaagtcagc ct
//
LOCUS       DASSFQ010000022        14509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_161308, whole genome shotgun sequence.
ACCESSION   DASSFQ010000022 DASSFQ010000000
VERSION     DASSFQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14509
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161308"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFH96_00675"
     CDS             complement(<1..564)
                     /locus_tag="VFH96_00675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET6972500.1"
                     /translation="MTAGISAIIPTLGRPEMLKLCLESLTKQTVPISEVILVHCGDDK
                     ATQTLAKETRWSDAGLSVRYFHYPERNCAAQRNFAVARASYDNLLLIDDDVEVEACWT
                     EELFKPIWNDSKVGATMGSVVNQPMATPTFFWRIYRTVLHGKTEGFAPGRLVGAALPN
                     GFPTTARELMSCEWIRGGASALRREAFE"
     gene            complement(561..1424)
                     /locus_tag="VFH96_00680"
     CDS             complement(561..1424)
                     /locus_tag="VFH96_00680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6972501.1"
                     /translation="MKSLLDRIAHHLLFRAQSHPQLTDRWGYHIRPIHYYEPIPDFRA
                     ITLEQITRRREFPGIDFRWDEQLALLNELTAYRDELNTLEFKFDNGFFSSFDAAVYYS
                     LIRHLKPQRIIEIGGGYSTQLAAKALTRNGSGKLICIEPYPERLNGVGPSVELIQKRV
                     EEIDVDFFSCLEAGDILFIDSSHVVKFGSDVCYEFLQLLPRLAPGVWVHVHDIFFPHD
                     YPAEWLLERRTAWNEQYLLEAFLSFNEKFQVALANYWLCLDHRDEAARLWPNALSADY
                     GASSLWMKRIA"
     gene            complement(1432..2700)
                     /locus_tag="VFH96_00685"
     CDS             complement(1432..2700)
                     /locus_tag="VFH96_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972502.1"
                     /translation="MSNVTIRCEGLSKRYRIGASQERYKALRDVITDAATAPFRRIRG
                     ASRNGHASANGQAAIDNSYIWALDDISFEINTGDLVGIVGRNGAGKSTLLRILSRITK
                     PTRGRAEVHGRVGSLLEVGTGFHPELTGRENVFLNGAILGMRKLEISRKFDEIVAFAE
                     VEKFIDTPVKRYSTGMYMRLAFGVAAHMETEILIVDEVLAVGDAQFQKKCFQRMSEIG
                     RHGRTILFVSHNMSALRNICKQALVIDHGKLLAQGEINHTIDHYLAQVSDDSQDARVE
                     TPSFIVTSVEIRSETSPVIKTFDPVCISVSFISKTDIADPALFVSILTMDSRRLASLD
                     FRDFANAAPLQSGEGCELGFTIESLPLLPGSYQLEIYLKNSRDLVELVPRAYQFEIAE
                     TQVYGGRKLDNWFGRVGLKAKAWGSSSRDS"
     gene            complement(2700..3548)
                     /locus_tag="VFH96_00690"
     CDS             complement(2700..3548)
                     /locus_tag="VFH96_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972503.1"
                     /translation="MQSLRRPPLTVPSFHIEPSTRWPSLGLRELWDYRELLYFLTWRD
                     IKVRYKQTVLGAIWVIIQPLFMMLVFSLFFGRLARVPSDGVPYPVFTFCALLPWQLFA
                     HALTESSNSLVGNQNLITKVYFPRLVVPISAVLGGLVDFAIAFVILLVLMLIYGIVPG
                     WQIVFVPALVLLAVMAALGVGLWLSALNVQYRDVRYTINFMVQFWLFATPVAYPSSIV
                     PEKWRLLYGLNPMVGVVDGFRWALLGKPDSLGLPLLISVVVVLMLLVGGLYYFRRLEQ
                     QFADIV"
     gene            complement(3667..6114)
                     /locus_tag="VFH96_00695"
     CDS             complement(3667..6114)
                     /locus_tag="VFH96_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET6972504.1"
                     /translation="MSDNLFDLYKIAIMNPTDKLAKPEPRVEVLDRPIEPPQSYNYGV
                     DPNAEHEVHLLDYWRAIRKRLWLVIGIVALVTMLAVVYVARKPDLYEAQSRVQVDLEE
                     SGNLVNNTRPIYGPTDDPIYFNTQLQILVSPGLMRRVVRTLDLEHNPDFFKGAQRQST
                     WQTFLRMVGLGSKPPEAPKQPDQLPLTTSFAQATAREDLNEAKRLAPYVNTILAGLKV
                     DPVKETRGYYKETRLIDIKFTHTDPNVAEKVVNAIAEVYVFWNLEKRTEANSTTGDFL
                     QKRIAELQQQIRTSEERLVNYAKNNQIISLDPNQNTVVERLAGLNQQLLQAENERKTA
                     EAAYNAAKTPDAANALVDEAAKQNSDVEAKLIELRQKRALLLVDATEEAPEVKEVDQQ
                     ITELDKQLKDLRSRKSATLMTNLNTRYQQALEREQSLRKAFEQQRAQTLSQNEAAINY
                     RIIQQEIETNKSLLNGLLQGAKENDVVLAGKPNNISIVDYALTPDTPVGPNRTRTVIA
                     AFFLSIGLGLGLALFFEYLDDTVHSTEEVERVLHLPALAVIPSVGSATRRRVLPGKSK
                     TTALQKSSNGNGNSNSELLQNVDGRSPLAEAYRHLRTSVLLSTAGRAPKSLLVTSSLP
                     GEGKTTTAVNTAISLAQTGASVVIIDADMRRPRLQSIFDMHGQLGLSSILSSDASADD
                     MLSMVTADQESGLSVLTSGPIPPNPAELLGSDQMRRLIATLQANYTHVVVDSPPVSSF
                     TDGVLISTMVDGVLLVVHGGKSSRHIVRRSKQLLSDVGAKIFGVVLNNVNLQSHDYYY
                     YQSYYGQKYYQKAEGSN"
     gene            complement(6180..7082)
                     /locus_tag="VFH96_00700"
     CDS             complement(6180..7082)
                     /locus_tag="VFH96_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET6972505.1"
                     /translation="MKIIITLLFLLVPVQAQDDRYRIGPGDVLDIRVYNRPQLSREAV
                     RVEGNGMIRMPLVDNDIQAICLTEGELAKEISTRYARYYKNLQVDVFIKEYHSKQVAV
                     IGAVNEQSRFELQRRVRLLELLTYAKGPSAKAGQTINIVHSTAVSPCKQQTDDTTAFS
                     SYKLSDVLQGDPKSNPYLEAGDIVTLPEADQVYVVGNVFMPLTIALKEPITLTRAIAM
                     AGGLKQDTKKDKIRILRQEPGTTIRKEITVNLSAIEKKSSEDLALAPNDIVDVPTSAG
                     KSFLRSLVQGVVPGVGQLPVRVVP"
     gene            complement(7154..7417)
                     /locus_tag="VFH96_00705"
     CDS             complement(7154..7417)
                     /locus_tag="VFH96_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972506.1"
                     /translation="MKKKRKKKTALVICRDRKQFWTTQTQFWQWVREGIVVKTGEQPL
                     TGLFRREHEELMVLLSNTVLNLAHPNHMREALLSRRLGMSGHR"
     gene            complement(7575..8135)
                     /locus_tag="VFH96_00710"
     CDS             complement(7575..8135)
                     /locus_tag="VFH96_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HET6972507.1"
                     /translation="MQFLTNSLQEHLATIQNLLDASLPEIEQSGQMICEALSSGRKVL
                     LCGNGGSAADAQHIAAELVGCYEKQRRSWPAIALTTDTSALTAVSNDLGYEQVFARQV
                     AGLAQTGDVLIAISTSGKSINILRAAERARELGCKTIALTGATAGPLGNLCDVAVAVP
                     SSRTSRVQEAHITIGHLWCEMVDQIS"
     gene            8218..10836
                     /gene="mutS"
                     /locus_tag="VFH96_00715"
     CDS             8218..10836
                     /gene="mutS"
                     /locus_tag="VFH96_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET6972508.1"
                     /translation="MSNSNSTPMLRQYQELKQQHPGTLLFFRLGDFYELFFDDAVTGS
                     RELQITLTARHKESSNPIPMCGVPHHSAANYIARLVRKGYRVAICEQTEEAGKTKKLV
                     RREVVRIVTPGTPIDPQLLEAREPVFLAAVCSAGESIGAAFLDISTGEFRVTQETGRD
                     SWMRIRAALESYAPRELLFPASLGALIKASLTGKVQTAPLPLASSNGGAAHSSREDFG
                     FEVALTPLDDWQWTKDDCFTLLLEHFKVKTLDGYGLSRKDEAIRAAGACLRYARETQR
                     AAAEHVTDLVYFEPQDHLVLDSVTVRNLELVESLAGGSGRSLLQVIDETVTGMGARLL
                     RAWLLRPCVKRGEAEARLAAVAVLLASQLRRDKLRALLKEVSDLERVVGRISLGSATP
                     RDLFAMLRSLNQVPAIREVLSGLEASLIEVLVENTDELPDIRDLIERSINDDPPIKLS
                     DGDTIRAGYSAELDELRSVSRNAKQIIAALEATERARSGIGNLRIRFNGVFGYYIEVS
                     KANAARVPAEYERRQTLANAERFTTPELRDWEKKVLGAEERIVQIETELFTDVCRQIA
                     AETKRIQMTARALAALDVLASSAEVAARRRYTRPSLHDGDELEIVQGRHPVIEAFIDE
                     PFVPNNLYLNNSTDRLLIITGPNMGGKSTVLRQTAIISILAQTGCFVPAERARLPLLD
                     RIWTRVGASDDLTRGRSTFMVEMTETAAILHSATPRSLVLLDEIGRGTATFDGLSIAW
                     AVAEYLHNSADHAAKTLFATHYHELTELAERLPGAQNYQITATEREGEVVFLHRLERG
                     RASKSYGIEVARLAGLPPAVIATARDVLARLERYELDVFTADDELGKAARRRAAAQAT
                     LFDLTTTDDTDLKSGS"
     gene            complement(10853..14440)
                     /locus_tag="VFH96_00720"
     CDS             complement(10853..14440)
                     /locus_tag="VFH96_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972509.1"
                     /translation="MHKYGTTETLEKLKDGKDAVGLSCTSCHGGNPVSRLKDDAHVRP
                     MFPREWGRTGGNPERSNTLLVRESWEFVRFVNPGDLRVVGRTCAGSACHEVESKNVSR
                     SMMTHGAMLWGAALYNNGSFHLKDPRFGESYSESGKPQTLLQAATDEQRRTKGWLSLL
                     DPIPRWEISQPGNVLRVFERGGKRRLEVGLPDKDEDPGKPDKGLSARGFGTAQRTDPV
                     FLGLQKTRLLDPTLNFLGTNDHPGDYRSSGCSACHVIYANDRDPAHSASYHQAGNLGR
                     SQTADVSIQKSESGHPIKHQFTSQIPTSQCMVCHMHPGTNMVATYLGLTWWDNESDGA
                     KMYPSTQHDPSQDEEQQKLDRNPEAASLRGLWSSFDFLKDTGTPAFNALLRNTQFADF
                     HGHGWLFRKVFKRDRKGNLLDAQGNIVSASDPAKFSKAVHLNDIHAERGMHCVDCHFR
                     QDSHGDGNLYNEPRAAIEIACEDCHGTIKQKSTLFTSGPAAAVATGTAAERRKTQNQP
                     LAGQDLTRIRVRDAEGARIPLFQRVTRDRKRKDEKGVDVDLKTGDLMQNSMVVPGRWW
                     RVPQTLDTVTPGSRDYNEKSRYAKTIQKDNATWGDVPADNNRLAHRDANMTCVACHSS
                     WVTSCFGCHLSMQANRKMPNRHNEGGNTRNFTQYNFQVLRDDIFMLGRDGTVTGNRVA
                     PVRSSSAVLVSSQNQNREWIYSQQQTTSAEGFSGQTFNTHVPHTVRSTETKTCSDCHV
                     SQQNDNNAWLAQLLLQGTNFVNFMGRYVYIAADDALEVVVATEQSEPQAVFGSTLHKI
                     AYPMNYDRFLRGGSELKTFYEHVGNRKVLQVQLRGEYAYVAAGEGGLRVYDVAQIDQK
                     GFSERIVTAPVSRFGQKFWVKTKYAVAVAAPTTLAVDPARTKRPENEEQAIHPLYAYL
                     YVVDKYEGLILVNAATLLDGDPLNNYLKRALTFNPDGVLNDANNITIGGNQVYITTSK
                     ELVIVNINDPLNPRLINRIALKHPRSVAIQFRYAFVVDDDGLKVIDVTTPEQARVVEG
                     AFVPLKHAHDVYVARTYAYVANGPEGIAIVDVEQPEHPRLDQTYNADGKLNDVHQVKV
                     AMTNASLFAYVADGKNGMHVLQLTSPETMPEYAGFSPRPRPMLIATFKTKGEALAISK
                     PLDRDRAVDESGNQISVFGRRGARPFTYEEILRMLRTNDGKGDWFRVKQ"
BASE COUNT         3058 a         4106 c         4028 g         3317 t
ORIGIN      
        1 ctcgaatgcc tcgcgtcgca gcgcactcgc gccgccccgg atccactcgc atgacatcag
       61 ctcacgcgcc gtcgttggaa agccgttcgg aagagccgct ccgaccaggc gaccgggtgc
      121 gaagccttct gttttaccgt gcaacacggt tcgatagatt cgccagaaga atgtaggcgt
      181 cgccattggc tggttcacta cactgcccat cgtcgcgccc actttcgaat cgttccagat
      241 cggtttgaag agctcctcag tccagcaagc ttcgacttcc acatcgtcgt cgattagcaa
      301 aaggttgtcg taacttgcac gcgccacagc aaaattgcgc tgggctgcac aatttctttc
      361 cggatagtgg aaataacgca cggataatcc cgcgtcgctc cagcgggttt ctttggcgag
      421 agtctgtgtc gctttgtcat ctccgcaatg aacaaggata acttccgaga tgggaactgt
      481 ttgtttggtg agactctcga gacagagttt cagcatttcc ggtctgccga gtgtcgggat
      541 gattgcagaa attcctgctg tcatgcgatc cgtttcatcc aaagactcga agcgccgtaa
      601 tcagcagaaa gggcgttggg ccaaagacgc gcagcttcgt cccggtgatc aagacagagc
      661 cagtaattcg cgagtgcgac ctggaatttt tcgttgaagg acaagaacgc ttcgagcagg
      721 tattgctcgt tccaggctgt ccggcgctcg agcaaccact cagccgggta gtcgtgcgga
      781 aagaagatgt cgtgtacgtg tacccaaacg ccgggcgcga gccgtggcag taactggaga
      841 aactcgtaac aaacgtcgct gccaaacttc actacatgac tggaatcgat aaagaggatg
      901 tcgccggctt cgagacacga gaagaaatca acgtcgattt cttcgacccg tttttgaatc
      961 agctcgacgc tcggaccaac gccattgagt cgttccggat aaggctcgat acagatcaac
     1021 ttgccgctgc cgttccttgt taatgcttta gccgcgagct gggttgaata accgccaccg
     1081 atctcgatga tcctttgcgg cttgagatgg cgaatcagtg aatagtagac cgccgcatcg
     1141 aaactgctga agaaaccgtt gtcgaactta aactcgagcg tgttcagctc atcgcgatat
     1201 gcagtcaatt cgttgagaag cgctaactgc tcgtcccacc tgaaatctat ccccggaaac
     1261 tctcgacgcc gcgtgatctg ctccaatgtg atcgcgcgga agtccgggat cggctcgtaa
     1321 taatgaatcg ggcggatgtg atagccccag cgatcggtga gctgcggatg gctttgtgcc
     1381 cggaacaaaa ggtggtgcgc tatgcgatcg agaagtgatt tcacgcaaca atcatgaatc
     1441 tctgctggaa cttccccatg ccttagcttt caaaccaacc cggccaaacc agttgtccag
     1501 ctttcgtccg ccgtagacct gcgtttccgc aatctcaaat tgatatgcgc gtggaacaag
     1561 ctctaccaaa tctcgagagt tcttcaggta aatctccaac tgataagacc cgggcaagag
     1621 cggcaacgac tcaatcgtga aaccaagttc acaaccctca ccggactgca gcggcgctgc
     1681 gttggcaaaa tccctgaaat ctaggctggc cagtcgacgt gagtccatcg tgaggatcga
     1741 aacaaaaaga gctggatctg cgatgtcggt tttggagatg aaactgacgc tgatacacac
     1801 cggatcgaac gtcttgatta ccgggctggt ctcggatctg atctcaactg aagtcacgat
     1861 gaaagaagga gtctcgaccc gcgcatcctg agagtcgtca cttacctgcg ccagataatg
     1921 atcgatggtg tgattgatct caccctgggc caggagcttg ccgtggtcta tcaccagtgc
     1981 ctgtttgcag atattccgca gcgccgacat gttatgactg acaaacagga tcgtgcgacc
     2041 gtgcctccca atctcactca tgcgctgaaa acacttcttt tgaaactgcg cgtcgccaac
     2101 ggccagcact tcgtcgacga tcaaaatctc agtctccata tgcgccgcca cgccaaacgc
     2161 gagccgcatg tacattccgg tcgagtaacg ctttacgggt gtgtcaatga acttctcaac
     2221 ttccgcgaag gccacaatct catcgaactt gcgactgatc tccaactttc gcatgccaag
     2281 tatggcaccg ttgagaaaga cgttttcgcg gccggtcagc tcaggatgga aaccggtgcc
     2341 aacctccaac aacgatccga cccgtccgtg cacttctgct cgtccgcgcg taggtttggt
     2401 tatgcgcgag agaatcctga gcagcgtgct cttgccggcc ccgttgcgac caacgatgcc
     2461 gaccaggtcg ccagtgttga tctcgaacga gatatcgtcg agtgcccaga tgtaactgtt
     2521 gtcaattgcg gcctgtccgt ttgcgctcgc atgcccgttg cgcgacgcac cacgaatacg
     2581 acgaaacggt gctgtggctg catcggtgat cacatcgcgc agcgccttgt agcgctcctg
     2641 tgatgctccg atgcgatagc gcttgctaag accttcgcaa cgtatcgtga cgttcgacat
     2701 cagacaatat cggcgaattg ctgctcgagg cgacggaagt agtagaggcc acctacgagc
     2761 agcattaaga caacgactac ggaaattaac agcggcaggc caagcgagtc gggtttaccc
     2821 agcaaagccc agcgaaatcc atccacgaca ccgaccatcg gattcagtcc gtacagtagt
     2881 ctccatttct ccggaactat cgagctcgga tacgcaaccg gcgtcgcaaa caaccagaac
     2941 tggaccatga agttgatggt gtaacgtacg tctcggtatt gcacattgag ggctgagagc
     3001 caaagaccaa cgcccaaagc agccatcact gccagcagga ccagcgccgg aacaaacacg
     3061 atttgccaac cgggcacgat gccataaatc agcatcagca ccagcagaat cacgaaggcg
     3121 atggcaaaat cgaccaggcc gccaagcacg gcagaaatgg gcacgaccag gcgcggaaaa
     3181 taaactttag tgatcagatt ctgatttcct acgaggctgt tactcgactc cgtcaaggcg
     3241 tgcgcgaaga gctgccacgg caagagcgca cagaaagtga ataccggata aggaacgccg
     3301 tcagaaggca cgcgagccag ccggccaaag aacagactga atacaagcat catgaacaac
     3361 ggttgaatga tgacccagat ggccccgagt acagtctgct tgtagcgtac cttgatatcg
     3421 cgccaggtca ggaaatagag aagctcgcga tagtcccaca actcgcgcaa gccgagtgag
     3481 ggccagcgag ttgacggctc gatgtgaaaa gaagggacag tgagtggcgg tctgcgaagg
     3541 ctctgcacaa gattttaaga tcttctctgc ggttctctgc gtatctctgc gttctctgcg
     3601 tttaaatttt cttttaacgc agagggcgca gagatacgca gagagccgaa gagaagatcg
     3661 atcacgttag ttgcttcctt ctgctttctg gtaatacttc tgtccgtaat aactctggta
     3721 gtaatagtag tcgtgagatt ggagattgac gttgttcagc accacgccga agatcttcgc
     3781 gcccacatcg ctcaacagtt gtttagatcg ccgcacgatg tgcctcgagc tcttgccgcc
     3841 atgcaccacg agtaacacgc cgtcaaccat cgtcgagatc aacacaccgt cagtgaatga
     3901 gctcaccggc ggcgaatcaa cgacaacgtg cgtgtagttt gcctgcagcg tcgcgattaa
     3961 tctgcgcatc tggtccgagc cgagcagctc agccggattg ggcggtatcg gacccgaggt
     4021 cagcacactc aatccgcttt cctgatctgc cgtgaccatc gacagcatat cgtctgcact
     4081 cgcatcactc gaaagaatcg agctgagacc cagctgcccg tgcatgtcga agatcgattg
     4141 caggcgcggt cgacgcatgt cggcatcgat gatcaccacg ctcgcgcctg tttgcgcgag
     4201 actgatcgcc gtgttgaccg cagtcgtagt ctttccttca cccggtagac tcgacgtgac
     4261 gagcagagac ttcggcgcgc ggccggcagt cgagagcaac actgacgtgc gcaggtgtcg
     4321 ataagcttcc gcgagcggcg aacgaccatc gacattctgt agcaactccg aattgctgtt
     4381 gccgtttccg ttgctgctct tctgcaaagc cgtcgtcttg gattttccgg gaagcacgcg
     4441 gcgtcgcgta gcgcttccaa ccgagggaat cacagccagc gccggcaagt gcaacacgcg
     4501 ctcgacttcc tcagtcgagt gcacagtgtc gtcgaggtat tcgaagaaga gtgcgagacc
     4561 gagtcccagt ccgatcgata ggaaaaatgc tgcgatgacc gtgcgggtgc ggtttggacc
     4621 aacaggcgtg tcgggcgtga gagcgtagtc gacgatcgaa atgttgttcg gcttgccggc
     4681 gagtacgacg tcattctctt tcgcgccttg gagcaacccg ttcagcagag acttattcgt
     4741 ctcgatctcc tgttgaatga tgcgatagtt gatcgccgct tcgttctgcg agagcgtttg
     4801 cgcgcgttgt tgctcgaacg ctttgcgcag cgattgttcg cgctcgagtg cctgttgata
     4861 gcgagtgttc aggtttgtca tgagggtcgc cgacttgcga ctgcgcagat cctttagctg
     4921 tttatcgagc tcggtgatct gctggtcgac ttccttcact tccggagctt cttcggtcgc
     4981 atcgacgagc aacagcgcac gcttttgccg caactcgatc agtttcgctt cgacgtcgct
     5041 attctgtttg gcagcctcgt cgacgagcgc attcgccgca tcgggcgtct tggcggcgtt
     5101 gtaagcagcc tccgccgtct tccgctcgtt ttctgcctgc aacagttgct ggttcagacc
     5161 ggcgagacgc tcgaccacgg tgttctggtt cggatcgagc gagatgatct gattgttctt
     5221 ggcgtagttg acgagtcgtt cttcagaggt gcgaatctgt tgttgcagct cggcgatgcg
     5281 tttctggagg aagtcgccgg tcgtactgtt ggcttccgtg cgtttctcga gattccagaa
     5341 gacgtaaact tcggcgatcg cgttcacgac tttctccgcg acattcggat ccgtgtgcgt
     5401 aaacttgata tcgatgagac gtgtttcttt gtagtaacca cgcgtctctt tcaccgggtc
     5461 gactttcaaa cccgcgagga tcgtattgac gtacggcgcg agtcgcttcg cttcgttgag
     5521 atcttcacgt gcggttgcct gggcgaagct cgtcgtgagc ggtagctggt ccggttgctt
     5581 cggagcttcc ggcggcttgc ttccgagtcc gaccatgcgc aggaacgtct gccacgttga
     5641 ctgtcgttgc gcgcctttga agaaatcggg gttgtgttcg agatcgaggg tgcggacgac
     5701 gcgtcgcatc aatccggggc tgacgagaat ctggagctgc gtgttgaagt agatcggatc
     5761 gtcagttgga ccataaatcg ggcgcgtgtt gttgaccaga ttgcctgatt cttcgagatc
     5821 gacctgcacg cgtgactgcg cttcgtacaa gtccggcttg cgcgcgacgt acaccaccgc
     5881 cagcatcgtg acgagggcca cgatgccgat gacgagccag agacgtttgc gaatcgctcg
     5941 ccagtaatcg agcaggtgaa cttcgtgctc ggcgttggga tcgacaccgt aattgtaaga
     6001 ctgcggcggc tcgatgggcc gatcgagaac ttcgactctg ggctcaggct ttgcgagctt
     6061 gtcagttgga ttcatgatcg cgatcttata taggtcgaat aagttgtccg acatgcttca
     6121 gcttgtcggt atgtcgaggc cgacaagctg aagcatgtcg gacattcctc taggccctat
     6181 tacggcacca cgcgcacggg tagttggccc acgccaggca cgacgccctg gaccaggctg
     6241 cgcaggaagc tcttccctgc tgatgtggga acgtcgacga tgtcgtttgg cgccagcgcg
     6301 agatcctcag aactcttctt ctcgatcgcg gagagattga cggtgatctc ttttctgatc
     6361 gtcgtgccgg gttcctgacg caggatgcgg atcttgtctt ttttcgtgtc ctgcttgagc
     6421 ccgcccgcca ttgcgatcgc gcgtgtcaac gtgatcggct ctttcaaagc aatcgtgagc
     6481 ggcatgaaga cgttgccgac tacgtagact tgatcggctt caggtaacgt cacgatgtcg
     6541 ccggcttcga gatacgggtt tgatttcgga tccccctgga ggacgtcgct cagtttgtaa
     6601 ctgctgaatg cggtcgtatc gtcagtctgc tgcttgcagg gtgatacagc cgttgagtgg
     6661 acgatgttga ttgtctgacc ggctttcgcg gatggtcctt tggcgtaggt cagtagttct
     6721 agtaaacgca cgcgtcgctg tagctcaaag cggctctgct cgttgacggc gccgatgacc
     6781 gcgacttgtt ttgagtggta ttccttgatg aagacgtcga cctgaaggtt cttgtagtaa
     6841 cgagcgtaac gagtggagat ctctttcgct aattcacctt cagtgagaca gattgcttga
     6901 atgtcgttat cgaccagcgg catgcgaatc atgccgttgc cctcgacgcg aaccgcttca
     6961 cgcgaaagct gcgggcgatt gtagacgcgt atgtctagaa cgtcgcccgg gccgatgcgg
     7021 tagcgatcgt cctgcgcctg gacaggtacg agaagaaata aaagggtaat aattatcttc
     7081 atttagttag ccgcggatta cacggataaa aaaccggatt ggatccgtga aaatcggctt
     7141 tcgaaaatcc gttttatctg tgtcccgaca ttccgaggcg ccgggataga agggcttcgc
     7201 gcatgtgatt cggatgtgcc agattcaaaa ctgtgttact caacaacacc atcaactcct
     7261 cgtgctcgcg cctgaagaga cccgtgagcg gctgctcgcc cgtcttcacg acgatgcctt
     7321 ccctcaccca ctgccagaac tgcgtctgtg tcgtccaaaa ctgtttccgg tcgcggcaga
     7381 tcacgagcgc tgttttcttc ttccgctttt tcttcattca gacacacctc aacccttgcg
     7441 tgttgttctc acacagaaac ccgttgggga agcctctctc gcgaggcagc atccagttgt
     7501 caacttgtcg ggcgcgaaac agaattcatc acgcccgtat tactgtcaag ttagtaaatg
     7561 acaaaaccag ttgttcacga aatctgatcg accatttcgc accagaggtg tccgatggtg
     7621 atgtgagctt cctgtacgcg tgaggtgcgg cttgacggga cagccacagc gacatcgcac
     7681 aggtttccca gcggcccggc ggtcgcaccg gtcagtgcga tcgttttaca tcccaactcg
     7741 cgcgccctct cagccgcacg tagaatattt atcgacttgc cactcgtgct aatcgcgatc
     7801 agcacgtcgc ctgtttgcgc gagtccggcg acctggcgcg caaacacttg ttcgtagcca
     7861 aggtcgttgc tgacagccgt cagcgctgaa gtgtcggtcg tgagcgcgat ggccggccac
     7921 gagcgtcgtt gcttttcgta acaaccaacc agctcggcgg cgatgtgttg tgcatccgct
     7981 gcgctgccgc cgtttccaca caacaaaact tttcgaccgc tcgataatgc ttcgcagatc
     8041 atctgacctg attgttcaat ttccgggagt gatgcgtcga gtaagttttg aattgtggcc
     8101 aggtgctctt gcagcgagtt ggtcaaaaat tgcacgtgtg gaaattaaca cagttttccg
     8161 ccgttgccca cggatatccg tgtattttgt gttaccctgt gcagccactc acaacatatg
     8221 agcaattcaa attcaacgcc gatgctgcgt cagtaccagg aactgaagca gcaacatccc
     8281 gggacgctgt tgttttttcg tctcggcgac ttttatgaac tgtttttcga cgacgcagtg
     8341 accggctcac gcgagttgca aatcacactc acggcgagac acaaagagag cagcaatccg
     8401 attccgatgt gtggcgtgcc gcatcattct gcggcgaact acatcgcgcg cctcgtgcgc
     8461 aaaggatatc gcgtcgcgat ctgcgaacag actgaagagg cgggcaagac gaagaagctt
     8521 gtacggcgcg aggtagtaag aatcgtgacg ccggggacgc cgatcgatcc gcagttgctg
     8581 gaagcgcgcg agcctgtctt tctggcggcg gtttgcagcg caggtgaatc gattggcgcg
     8641 gcgtttctgg atatttccac aggtgagttt cgggtgacgc aggagacggg gcgcgattcg
     8701 tggatgcgca ttcgtgcggc cctcgaatcg tatgcgccac gcgagttgtt gtttccggca
     8761 tcgctcggcg cgcttattaa ggctagtctc accggaaaag tgcaaaccgc gccgctgcca
     8821 ttggcaagct cgaacggcgg cgctgcacat tcttctcggg aagatttcgg gtttgaggtg
     8881 gcgctgacac cgctcgacga ctggcagtgg acgaaagacg attgcttcac gctcctgctt
     8941 gagcatttca aggtcaagac gctcgacggt tacggactgt caaggaaaga cgaagcgatc
     9001 cgtgcagcgg gggcgtgttt gcgttacgcg cgcgagactc aacgggcagc cgctgagcac
     9061 gtcaccgatt tagtttattt cgagccgcag gatcatctcg tgctcgatag tgtcacggtt
     9121 cggaatctcg agttggttga gtcgctcgct ggcggcagtg gtcgatcgtt gttgcaagtg
     9181 attgatgaaa ccgtgactgg catgggcgcg cgattgttac gcgcgtggtt gctgcggccg
     9241 tgcgtgaaac gtggcgaggc tgaggcacga ctcgcagcag tagccgtttt actcgcatct
     9301 caactaagaa gagacaagct gcgtgcattg ttgaaggaag tttcggatct cgaacgggta
     9361 gttggtcgca tcagtctcgg ctctgctact ccgcgcgatc tgtttgcgat gttgcgctcg
     9421 ttgaaccagg tgccggcgat tcgcgaagtg ttgagtggac ttgaagcatc gctgatcgag
     9481 gtgctggtcg agaatacaga cgagctgcct gacatccgcg atctcatcga acgttctatt
     9541 aacgacgatc cgccaataaa gctcagcgac ggcgacacga ttcgcgcagg ttattcagcc
     9601 gagctcgacg agttgcgatc agtcagccgc aacgcgaaac agatcatcgc ggcgctcgaa
     9661 gccaccgagc gtgcgcgcag tggaatcggc aatctccgaa tccggtttaa tggcgtgttc
     9721 ggctactaca tcgaagtgtc gaaagcgaat gcggcgcgtg tgcccgcgga gtatgagcga
     9781 cgccagacac tcgcgaatgc agaacgcttc accacgcccg agctgcgcga ctgggagaag
     9841 aaagtgctcg gtgctgaaga acgcatcgtg cagatcgaaa cggagttgtt cacagatgtt
     9901 tgccgtcaga tcgctgcgga gacgaaacgc attcagatga ctgcacgcgc gctcgcggcg
     9961 ctcgatgtgc tcgcttcgtc tgcggaagtt gcggcgcggc gtcgttacac gcggccgtcg
    10021 ctgcatgacg gtgatgagct cgagatcgtt caggggcgac atccagtcat cgaggccttc
    10081 atcgatgagc cgttcgtgcc taataactta tatcttaaca actcgactga ccggctgctg
    10141 atcattactg gtccaaacat gggcggcaag agcaccgtgc tgcgccagac tgccatcatc
    10201 agcatactcg cgcaaactgg ttgctttgtt ccggctgagc gcgcccgact gcctctgctc
    10261 gatcggatct ggaccagggt tggcgcatcg gacgatctca cgcgcgggcg ttcgacgttc
    10321 atggtcgaga tgactgagac cgcggcgatc cttcacagcg cgacgccacg ctcgctcgtg
    10381 ctgctcgatg agatcggacg cgggacggcg acgttcgatg gactctcgat cgcgtgggcc
    10441 gtggccgagt acctgcacaa ctcagcggat catgcggcga agactctgtt tgcgacgcac
    10501 tatcacgagc tcactgaatt ggccgagcgt cttccgggcg cgcagaatta ccagatcacg
    10561 gcgacggaac gtgaaggcga ggtcgtcttt ctgcaccgcc tcgaacgcgg gcgcgcctct
    10621 aaatcttatg gaatcgaagt cgcgcgtctc gcaggattac caccggccgt aatcgccaca
    10681 gcccgcgatg tgctggcgcg tctggaacgt tatgaattgg atgtgttcac ggcagatgat
    10741 gaactcggca aggcagcgcg tcgcagagcc gccgcgcaag caactctctt cgatcttaca
    10801 accacggatg acacggattt aaaatccgga tcttgatccg tgttatccgc ggttactgtt
    10861 ttacccggaa ccagtcacct ttaccatcat tcgttcttaa cattctcaag atttcttcat
    10921 acgtgaacgg ccgagcacca cgccttccga acaccgaaat ctggttgccg ctctcgtcca
    10981 ctgcgcgatc gcggtccagt ggttttgaga tcgcgagcgc ttcgcctttt gtcttgaaag
    11041 tcgcgatcag cattggcctt ggccgtggac tgaagccggc gtactcgggc atcgtctcgg
    11101 gtgacgttag ttgcaggacg tgcatgccgt tttttccatc cgcaacataa gcaaacaaac
    11161 tcgcgtttgt catcgccacc ttcacctggt gcacgtcgtt gagtttgcca tcggcgttgt
    11221 acgtttgatc gagacgcgga tgctcaggct gttcgacgtc gacgatcgcg attccctctg
    11281 gaccattcgc gacgtaggca tacgtccgcg cgacgtaaac atcatgcgcg tgttttagag
    11341 gaacaaaagc cccttcaacg acgcgcgcct gctcgggtgt cgtcacgtcg atcactttca
    11401 atccgtcgtc atcgactaca aacgcatagc gaaactggat cgccaccgag cggggatgtt
    11461 tgagcgcgat tcggttgatt aaccgcggat ttaacggatc gttgatgttg acgatcacga
    11521 gttccttcga ggtcgtaatg taaacctgat ttccgccgat cgttatattg ttggcatcgt
    11581 tgagcacacc atcagggttg aaagtaagtg cccgcttcaa atagttgttc aacggatcgc
    11641 cgtcgagcag cgttgccgca ttcacgagga tcagtccctc gtatttatcg accacataca
    11701 ggtacgcata gagcggatga atcgcctgct cttcgttttc aggtcgcttc gtgcgtgccg
    11761 gatcaaccgc cagcgtcgtc ggtgcggcca cggcgactgc atactttgtc ttgacccaaa
    11821 acttttgacc gaagcgcgac acaggcgccg tcacgatccg ctcggaaaac cctttctgat
    11881 cgatctgcgc aacgtcgtag acacgcaacc cgccttcacc cgccgccacg tacgcatact
    11941 caccacgcaa ctgcacttgc agcactttcc gatttcccac gtgctcgtag aacgtcttta
    12001 actcgctccc gccacgcaga aaccgatcgt agttcatcgg atacgcgatc ttatgaagcg
    12061 tgctgccaaa caccgcttgc ggctccgact gctccgttgc caccacgact tcgagcgcgt
    12121 catccgccgc gatgtaaaca tagcgaccca tgaagttgac gaagttcgtg ccctgcaaca
    12181 acaactgcgc cagccacgcg ttgttatcgt tctgttgcga cacgtgacaa tcgctgcacg
    12241 tcttggtctc ggtcgagcgc accgtgtgtg gcacgtgcgt gttgaatgtt tggcctgaga
    12301 aaccctcagc agatgttgtt tgttgttgcg agtagatcca ctcgcgattc tggttctgcg
    12361 agctcaccag cacggccgac gaagaacgca ccggcgcgac gcgattgcca gttaccgttc
    12421 cgtcacgtcc gagcatgaag atgtcgtcgc gcaggacctg gaagttgtac tgcgtgaagt
    12481 tgcgcgtgtt gccgccttca ttgtgacggt tgggcatctt gcgattcgcc tgcatcgaga
    12541 gatgacaccc aaaacaactc gttacccacg atgagtgaca cgcaacgcac gtcatgttcg
    12601 cgtcgcgatg agccagccgg ttgttgtcgg caggaacgtc gccccacgtc gcgttgtctt
    12661 tctgaatcgt cttcgcgtaa cgcgacttct cgttgtaatc gcgactgccg ggcgtgaccg
    12721 tgtcgagcgt ttgtggcacg cgccaccagc gacccggcac gaccatcgag ttctgcatga
    12781 gatcgccggt cttcagatcg acatcgactc ccttttcatc tttccgtttg cgatcgcgcg
    12841 tgacgcgttg aaagagcgga atgcgcgcgc cttcagcatc gcgaacgcga atgcgcgtca
    12901 gatcctgtcc ggccagcggc tggttctgtg tcttgcgacg ctcggctgct gtgccggttg
    12961 caactgccgc tgccggacct gaagtaaaca gcgtcgactt ttgcttgatg gtcccgtggc
    13021 aatcttcgca cgcgatctcg atcgctgcgc ggggttcgtt gtagagattg ccatcgccgt
    13081 gtgaatcctg gcggaagtga cagtcgacac aatgcattcc gcgttcggcg tgaatgtcgt
    13141 tgagatgcac cgctttgctg aacttcgccg ggtcgctagc cgagacgata ttgccctgcg
    13201 cgtccagcaa gttgcctttg cgatcgcgtt tgaagacttt gcgaaacagc caaccgtgac
    13261 cgtgaaaatc tgcaaactgc gtgttgcgca agagcgcatt gaaagcgggc gtgccggtgt
    13321 ctttcagaaa atcgaagctg gaccagagtc cgcgcagact ggcggcttcc ggattgcgat
    13381 cgagtttctg ttgctcctca tcctgactcg gatcgtgctg cgtcgacgga tacatcttcg
    13441 cgccgtcgct ctcgttgtcc caccacgtta gaccgagata agtcgcgacc atgtttgtgc
    13501 cggggtgcat gtgacagacc atgcactgcg acgtcggaat ctggctcgtg aactggtgct
    13561 tgatcggatg tcctgactca gacttttgaa tcgacacgtc agcggtttgc gagcgcccga
    13621 gattacccgc ttgatgatac gaagccgagt gcgccggatc gcgatcgttt gcatagatca
    13681 cgtgacacgc agaacatcct gaagagcgat aatcaccggg atgatcgttc gtaccgagaa
    13741 aattcagcgt cggatcgagc agtcgagttt tctgtaatcc gagaaacacg ggatcggtgc
    13801 gttgcgccgt gccaaagccg cgcgcgctga gtcccttgtc aggcttaccc ggatcttcgt
    13861 ctttatcagg cagcccaact tcgagcctgc gcttcccgcc acgctcaaac acacgcaaca
    13921 catttcccgg ctgcgagatc tcccaacgcg gaatcggatc gagcaagctc agccaaccct
    13981 ttgtgcgtcg ttgctcatcc gtcgctgctt gtaaaagtgt ctgtggctta cccgactcgc
    14041 tgtagctctc gccgaaccgc ggatccttca aatggaaact gccgttgttg tagagcgctg
    14101 cgccccagag catcgcgccg tgcgtcatca tgctgcgcga cacgttcttc gattcaacct
    14161 catgacacgc cgatcccgcg cacgtccttc ccaccacacg cagatcaccc ggattcacaa
    14221 acctcacaaa ctcccaactc tcacgcacca acaacgtatt cgatctttcc ggattcccac
    14281 ccgtgcgtcc ccactcacgc ggaaacatcg gcctcacatg cgcgtcatct ttaagtcgcg
    14341 aaaccggatt cccaccatga caactcgtac aactcaaccc caccgcatcc ttcccatcct
    14401 tgagcttctc gagcgtctcc gtcgtcccat acttatgcat cggctcaatc tgcccatgac
    14461 acgtcacaca cccctccgtc ctcaccggct gctgcgcctg cacagtcca
//
LOCUS       DASSFQ010000023         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1949300, whole genome shotgun sequence.
ACCESSION   DASSFQ010000023 DASSFQ010000000
VERSION     DASSFQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949300"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VFH96_00725"
     CDS             <1..1507
                     /locus_tag="VFH96_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HET6972510.1"
                     /translation="LRDKAAPAFIDWCIKSVDWNRFGVIGFSVVFQQTLASIALAAAL
                     KKHYPHIPIMMGGATFEDDIAEEIMKGCPQVDYIHCGDADETFPKAIRRIYSGESMKG
                     MPGIMWRDNGRVAYNGRAPNLSDMNKTPTPEFDEYFYARKQGGYEYHDDAHGVLLPIE
                     TARGCWWGVKNHCTFCGLNRAGMEFRSKRVENVIEQLDELSRRYGILDFNAIDNIIEP
                     EYIDKLFGQLSEANTDIRLHYEVRPSLSRVQLKHMRQGGLFSIQPGVESFSTHILKLM
                     KKHTTGVRNLELIKWSTYYGINNLYNILLRFPGETAEDYRAQCDVISKIHHFQAPWAI
                     AKARADRGSPMYTDPESQSISSLVPSPCYDYLYPKDRFDLNRVSYYFEHVMGNTLQDR
                     EYDEIFEAVDVWQSRWKQRPRPYLRYKKAWATILIEDGRNCSPRVATYSDDCATLYEY
                     CADARSRKDIAARFEDASWVEGALAEFVEKDLMIHLDNRYLSLALPENQFI"
     gene            1526..>3923
                     /locus_tag="VFH96_00730"
     CDS             1526..>3923
                     /locus_tag="VFH96_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HET6972511.1"
                     /translation="MLREAHSTTIASLPENFQAARAELLELLQRRGILYRSESQPILS
                     RDGTSARWMLDSLAVTLSPRGAELAGRCVLELLKRFDGRQIATYGLTGVPILQSCILQ
                     DNRYRGLLVRKERKQHGSLKLIEGVIDPSEPVILIDDSVSSGTCMTEGTQRLEEAGLR
                     VEGGICLVRFGWQNGYALMQERGYHMEALYDIWTDFIAHMDDEEQPLPNPSKWFPKFE
                     WTNDKAPEHLHPAELARFVISEYLSSNKLLRPPESLDKKYDSSGGAWVSIRSRASIYQ
                     RHARGGFWHFPGETSRSAAEDVVMASLSTAAGLPAGEEGLKIVNESAFAVTFFSALER
                     CNPGQLDNDRYGIVVRSLERRERMGGALPRMPGIVNAWRQFQHARIKNAKLVSFEPYE
                     LFRHEVLKVVEPGEKWQPTGVPAPEEVPWQKDKKICGHIAERARDLVLGQLLGRGETT
                     SPLASDLLPENVESIYLTIYIDGHLRGCMGQRLRSLDDDLKKIAGAALNDERFEGRSA
                     INADDVAVSVSFLFDPLEIGHAPPEEVVNYYRHGDQTLMVYQGNRVGLLLPFVASFWN
                     LDAVSFAKSVIQKAELTEPPYNWCRFDSATWFAGSEGVWATVGGFAAPRNDLLPIEEL
                     IERHGKLHVRYLLKHLREDGSFFSGYQPFHNRLFESVDTARQAHGAWVLARAYKAFGG
                     DDLKSAANKAIDNLLPQTFDTVAEPSFLLLALSNLTSNDPRRAAMNDLAASLWQSIEL
                     PHGRISTHNNPDDPSPEIYQDYFPGQVLLALAVACEQNVTPIDEERLRRSFQYYRHRF
                     R"
BASE COUNT          822 a         1038 c         1182 g          881 t
ORIGIN      
        1 tctgcgtgac aaggccgcgc ctgccttcat tgactggtgt atcaagagcg tcgattggaa
       61 ccgcttcggc gtcatcggct tttctgtcgt ctttcaacag acactcgcaa gcatcgcgct
      121 ggctgctgcg ttgaagaaac attacccgca catcccgatc atgatgggcg gagccacgtt
      181 cgaagacgat atcgccgaag agatcatgaa aggctgtccg caagtggatt acattcactg
      241 cggcgacgcc gacgaaacat ttcccaaagc gatccggcgc atctacagcg gcgaatcgat
      301 gaagggaatg ccggggatca tgtggcgtga taatggtcgt gtagcttaca acggccgcgc
      361 gccgaatctc tccgacatga acaagactcc gacaccggag ttcgacgagt atttctacgc
      421 gcgcaagcag ggcggctacg aatatcacga cgacgcgcac ggcgtgctgt tgccgatcga
      481 gactgcgcgt ggctgctggt ggggtgtgaa gaaccactgc acgttctgcg gtttgaatcg
      541 tgcgggaatg gagttcaggt cgaagcgcgt cgagaacgtg atcgagcagc tcgacgagct
      601 ttcgcgccgc tacggcatcc tcgatttcaa cgcgatcgac aatatcatcg agcctgaata
      661 catcgacaag ttgttcggtc agctcagcga agccaacaca gacattcgtc tgcactacga
      721 agtgcgacca agcttgtcgc gcgtgcagct caaacacatg cgccagggtg gactgttttc
      781 gattcagccg ggtgtcgaga gtttttcgac gcacattctg aagctgatga agaagcacac
      841 gacgggcgtg cgcaatctgg agttgatcaa gtggtctacg tattacggca tcaacaatct
      901 ctacaacatc ctgctgcgat ttccgggtga gactgccgag gattaccgcg cgcagtgtga
      961 tgtgatctcg aagatccatc atttccaggc gccgtgggcg attgcgaagg cgcgcgcgga
     1021 tcgcgggtcg ccgatgtata ccgatcctga gtcgcagtcg atcagcagcc tcgtgccgtc
     1081 gccttgttat gactatttgt atccgaagga tcgtttcgat ctgaatcgtg tttcttatta
     1141 tttcgagcac gtgatgggca acacgctcca ggatcgggaa tacgatgaga tttttgaggc
     1201 ggtcgacgtt tggcagagtc gctggaagca gcgtccgcgg ccatatctgc gttacaagaa
     1261 agcgtgggcg acgatcctga tcgaggatgg acgcaactgc tcaccgcgcg tggccacgta
     1321 ttcggatgac tgcgcgacgc tttacgagta ttgtgcagac gcgcgcagtc gcaaagacat
     1381 tgcggcgcgg ttcgaggatg cgtcgtgggt cgaaggcgcg ctcgcagagt tcgttgaaaa
     1441 agatctcatg atccatctcg acaatcgcta tctcagcctg gctctccccg aaaatcagtt
     1501 tatttaatcc gtgtaatctg tggttatgct gcgcgaagcc cattcgacaa cgattgcgtc
     1561 gctgccggag aattttcagg ccgcgcgcgc ggagttgctc gagctgctcc agcgccgcgg
     1621 gattctttat cgcagcgagt cgcagccgat cctttcgcgc gacgggacct cggcgcgctg
     1681 gatgctcgac tcactcgccg tcacgctttc gccacgcggc gccgaactcg ccggccgctg
     1741 tgtgctcgag ctactcaaac gcttcgacgg ccgtcaaatc gccacgtatg gtctaacagg
     1801 cgttcccatc ctccaatcgt gcattcttca ggataatcgt taccgcggtc tactcgttcg
     1861 taaagagcgt aaacagcacg gctcattgaa actgatcgaa ggcgtgatcg atccgagcga
     1921 gccggtgatt ctgatcgacg actcggtgag ctccggaacg tgtatgaccg aggggactca
     1981 acgactcgaa gaagcggggc tgcgcgtgga aggcgggatt tgtctcgtgc gtttcggctg
     2041 gcaaaacgga tacgcgctga tgcaggagcg tggctatcac atggaggcgc tctacgacat
     2101 ctggaccgac ttcatcgcgc acatggacga tgaagaacaa ccgctcccaa acccgtcgaa
     2161 gtggtttcca aagttcgagt ggaccaatga taaagcgccc gagcatctgc atcctgcgga
     2221 gctggcgcgc ttcgtgattt ctgaatattt atcttcgaac aagcttttgc gtccgccgga
     2281 gagtttagac aagaaatacg attcgtcagg cggcgcgtgg gtgagcattc gctcgcgcgc
     2341 gagcatctat cagcgtcacg cacgcggcgg gttctggcat tttcccggtg agacttcgcg
     2401 ttcggcagcg gaagatgtcg tgatggcttc gttgagcacg gcggctggtc tgccggctgg
     2461 tgaagaaggg ttgaagatcg tcaacgagag cgcgtttgcg gttacgtttt tcagtgcgct
     2521 cgagcggtgc aatccgggtc agctcgataa cgatcgctac gggatcgtcg tgcgcagcct
     2581 ggagcgacgc gaacggatgg gcggtgcgtt gccgcgcatg ccgggcattg tgaacgcgtg
     2641 gcggcagttt cagcacgcgc gtatcaagaa tgcgaagctc gtgtcgtttg agccgtatga
     2701 gttgtttcgt cacgaagtgc tgaaggttgt ggagccggga gagaagtggc agccgacggg
     2761 agttccggcg cctgaagaag taccgtggca aaaagacaaa aagatttgtg gccacattgc
     2821 ggagcgtgcg cgcgatctcg tgttgggcca gttgcttggt cgtggtgaaa cgacgtcgcc
     2881 actggcgagc gatcttttgc ccgagaatgt cgagtcgatc tacctgacga tctacatcga
     2941 cggacatttg cgcggctgca tgggccaacg gcttcgtagc ctcgatgacg atctgaagaa
     3001 aattgccggc gccgctctca acgacgagcg attcgaaggt agatcggcga tcaacgccga
     3061 cgatgtagct gtttctgttt cttttctctt cgatccgctc gaaatcggcc atgcaccgcc
     3121 cgaagaagtt gttaactatt atcgccacgg cgaccagacg ttgatggtct atcaggggaa
     3181 tcgcgtcggg ttactgctgc cgttcgtggc aagtttctgg aacctcgatg cggtcagttt
     3241 tgcgaagtca gtaattcaga aggctgagct gactgagccg ccgtacaact ggtgccgctt
     3301 tgattctgcg acgtggttcg ctggtagtga aggtgtgtgg gccacggtcg gtggtttcgc
     3361 agcgccgcgt aatgatctgc tgccgattga ggaattgatc gagcgacacg gcaagttaca
     3421 cgtacgatac ctgctgaaac acttgcgcga agatggttca ttcttttccg gttaccagcc
     3481 ctttcacaat cgtctgttcg agtcggttga tacagcgagg caggcccatg gcgcgtgggt
     3541 gctggcgcgc gcgtacaaag cgttcggtgg tgacgatttg aagagcgctg cgaataaggc
     3601 gattgataac ttgttaccgc agacgttcga tacggtagct gagccttcgt tcctgctgct
     3661 cgccctcagc aatctgacta gcaacgatcc gcgtcgtgca gcgatgaacg atctcgccgc
     3721 gagtttgtgg caatctattg agttgccgca cggacgaatc tcaactcaca acaatcctga
     3781 cgatccatcg cccgagattt atcaagacta ctttccggga caggtgctcc tcgcgcttgc
     3841 ggttgcgtgt gagcaaaacg tgaccccgat cgacgaagaa cggttgcgac ggagttttca
     3901 gtactaccgc catcgctttc gct
//
LOCUS       DASSFQ010000024         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_460513, whole genome shotgun sequence.
ACCESSION   DASSFQ010000024 DASSFQ010000000
VERSION     DASSFQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460513"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFH96_00735"
     CDS             complement(<1..470)
                     /locus_tag="VFH96_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972512.1"
                     /translation="MKSTRLSAIDAFFVAYQESSGALMQVGVEVELKGRINREDVERM
                     LSHLVRRWPPLGQRLDKPFFGLAWDGAPRLGEMLHVGETREGLHEWRNKALNPFVEPP
                     FQVLWIASEDEHVLAFRAHHAVVDGEGFFGVCAAALRAVTNLSNERLFGVRGGGG"
     gene            complement(467..925)
                     /locus_tag="VFH96_00740"
     CDS             complement(467..925)
                     /locus_tag="VFH96_00740"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HET6972513.1"
                     /translation="MFELIRSISIDVEKGRASGEATVPADHPLFADHFPGYPLLPGSL
                     VIELAAQVCGPLAEEYARTTSEDEHWAILGMVRDARFLRPAFLPATLVINAEISRSQP
                     SSISMNVTVTSDDERIMQAELVLMLLEAAPEWSAAIEERNNRLARWKEAS"
     gene            complement(913..1311)
                     /locus_tag="VFH96_00745"
     CDS             complement(913..1311)
                     /locus_tag="VFH96_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6972514.1"
                     /translation="MSARDTSGEVATLVRQTLALPETEPISAPQLFFYDLGFTSMDLL
                     DLLFRIEQHFQIEIPEGTIYHLARGELAEAEFAQDAVLTETGRERLMDLLYDTPKELF
                     PARIHATTLPRFCTVGAITRLVDHKLACSN"
     gene            complement(1308..2015)
                     /locus_tag="VFH96_00750"
     CDS             complement(1308..2015)
                     /locus_tag="VFH96_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6972515.1"
                     /translation="MNDRVTLVTGATGYLGSALVKGFADAGASLFLTGRNEERLKEFS
                     ARSFAADLTEENAAASVVNRVVEEAGRIDTLVINAGTLADGLVAGLTDDQLAKVYEVN
                     VWAAFRMLRAALKQMILQRSGSVVVISSTAAQRPNAGQAAYASSKAALEVLVRTTARE
                     TAKRGIRVNAVAPGLLAGGMSARILDDHMDQARALIPMGRPGEAEEVVPVVLFLASPL
                     SSYITGQVWCVDGGVTA"
     gene            complement(2041..3198)
                     /locus_tag="VFH96_00755"
     CDS             complement(2041..3198)
                     /locus_tag="VFH96_00755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET6972516.1"
                     /translation="MSDKRRVVITGTGALSALGATTHELWNGLLANRSGVRRVKRLVD
                     SGINVTTGGEVDAISPQDVDRDHVIANRAIDDALAESKRDAAECAFIWSTGLDTFQLG
                     HEGFVHRSAGRCFSNLASRFSGPRRMIAAACASGTQAIGEAFRLIQKGRLDACVAGGS
                     SVMLTPFYLIGFAGLQAVAIDNGDDPSLACRPFDKRRKGFALADGAGAVVLETHESAV
                     ARGARVFAEVIGFGVSQDAFDLNRPCDDGAGAELCMRRALADAVLSSEEIDAVNAHAT
                     ATYSGDLAEAAALRKVFAERWQNVPVSGSKGAIGHAMAAAGVLEAIVAAQTCATGIVP
                     PTVNLQQVDDGCELDHVIGEPRDVNAKTVLSVSFGMGGQNAGVILRRVSHG"
BASE COUNT          770 a         1066 c          801 g          654 t
ORIGIN      
        1 cccccccccc cccgcactcc aaagaggcgc tcattgctaa gattcgtgac tgcccgaagg
       61 gccgcagcac aaacgccaaa gaacccttca ccatcaacaa cggcatgatg cgcgcgaaaa
      121 gcaagtacat gttcatcctc gctcgcgatc cagagcacct gaaacggcgg ctcaacaaac
      181 ggattcaacg ccttgtttcg ccactcatgt aacccttctc tcgtctcacc aacgtgaagc
      241 atctcaccca ggcgtggcgc accatcccac gccagtccga aaaatggttt gtcgagtcgt
      301 tgacccaacg gcggccaacg acgcacaaga tgcgaaagca ttctttcgac atcctcacgg
      361 ttgatccgtc ctttcaactc gacttcgaca ccaacctgca tcaacgcacc ggacgactcc
      421 tgatacgcca caaagaaagc atcaatggct gagagtcggg tcgacttcat gacgcttctt
      481 tccaacgtgc gagccggttg ttgcgttcct cgatcgccgc cgaccattca ggagccgctt
      541 cgagcaacat cagcaccaac tcagcctgca taatgcgttc gtcgtccgaa gtcaccgtga
      601 cattcatgct gatgctcgaa ggttgcgaac gtgagatctc tgcattgatc acgagagtcg
      661 ccggcagaaa cgcgggacgc aaaaagcgcg catcacgcac cataccgaga atggcccaat
      721 gctcgtcttc cgaggttgtt cgggcatact cctcggcgag cggtccgcaa acctgcgctg
      781 ctaattcgat aacgagtgac ccaggcagta acggatatcc aggaaaatga tcggcaaaca
      841 gtggatgatc cgcaggcaca gtcgcctcgc cggaagcgcg gcctttttcg acgtcgatag
      901 atatcgatcg gatcagttcg aacatgccag tttgtggtcc acgagacgcg tgattgcacc
      961 gactgtgcaa aaccgaggca aggtcgttgc atgaatgcgc gccggaaaaa gttctttcgg
     1021 agtgtcgtac agtaaatcca tcagacgctc cctgccggtt tcagtcaaca ctgcatcctg
     1081 cgcaaactca gcttcggcga gctcgccacg cgcaaggtga tagatcgttc cttccggaat
     1141 ctcgatttga aagtgttgct cgatgcgaaa cagcaggtcg agcaagtcca tcgaagtaaa
     1201 accaagatcg tagaagaaga gttgcggcgc tgaaatcggt tcagtctcgg gtaatgcgag
     1261 cgtctgtcgc acgagcgttg ccacttcgcc ggatgtgtcg cgcgcgctca agcagttacg
     1321 cctccatcga cacaccaaac ctgaccggtt atgtacgagc tcagcggtga cgcaagaaaa
     1381 agcaccacgg gcacgacctc ttcagcctca ccgggccgtc ccatcggaat cagcgctcgt
     1441 gcctggtcca tgtgatcgtc gaggatgcgt gcactcattc cgcctgcaag cagtcccggc
     1501 gcaaccgcgt tcaccctaat ccctcgcttg gcagtctcgc gtgcagtggt ccgaactaaa
     1561 acctcgagcg cggcttttga cgacgcatac gcagcctggc cggcgttcgg acgttgcgcc
     1621 gccgtcgagg aaatgacgac cacacttcca ctacgctgca aaatcatctg tttcagcgcg
     1681 gctcttaaca ttcgaaatgc agcccagacg ttgacttcat aaaccttcgc aagctgatcg
     1741 tctgtcaaac cagcgaccag tccgtcagcc aacgtgccgg cattgataac cagcgtatcg
     1801 atccgcccgg cttcttccac cactcgattc acaacacttg cggctgcatt ctcttccgtc
     1861 aaatccgccg caaaactgcg cgccgagaac tcttttaacc gctcctcgtt tcttcccgtc
     1921 aaaaacaaac tagcgcccgc gtcagcaaag cccttaacca acgcacttcc aagataccca
     1981 gtcgcgccgg taaccagcgt gactcgatca ttcaatcccc aatcggattt tctatccgtg
     2041 ttatccgtgg ctcactctcc ttagaatcac gccagcattc tgcccaccca tgccgaaaga
     2101 aacagacaac accgtcttcg catttacatc acgtggctct ccgatcacat ggtccaactc
     2161 acaaccgtca tccacctgtt gcaaattcac ggttggcggc acgattcccg tcgcgcaggt
     2221 ttgagcagcg acaatagctt cgagcacgcc ggccgcagcc atcgcgtgcc cgatggcccc
     2281 tttcgatccc gacaccggaa cattttgcca acgctcggcg aacactttcc tcagcgccgc
     2341 tgcctccgcg agatcgccgg agtaggtcgc ggttgcgtgc gcgttaacag catcaatttc
     2401 ttcagaactc aggacagcat cagccagcgc acgacgcata caaagctcag caccagcgcc
     2461 gtcgtcacac ggtctattca aatcgaacgc atcctggctg acgccgaaac caatcacctc
     2521 tgcgaacact cgtgcgcccc gtgcaactgc gctttcgtgt gtctccaaca caactgcgcc
     2581 tgccccatcc gcgagtgcaa atcccttccg gcgtttgtca aaaggacgac acgccaacga
     2641 cggatcgtca ccattatcga tcgccactgc ctgtaatccc gcaaacccga ttaaataaaa
     2701 cggcgtgagc atcacactcg aaccgccggc cacgcatgca tcaaggcgac ctttttgaat
     2761 caaacgaaac gcttcgccaa tcgcctgtgt gcctgacgcg cacgcagcgg caatcatgcg
     2821 tcgtggacca ctaaatctgg acgccagatt cgagaagcaa cgtcccgcgg agcgatggac
     2881 aaaaccttcg tggccgagct gaaaagtgtc gagaccggtg gaccagataa atgcgcattc
     2941 ggcggcatcg cgtttcgact ccgcaagcgc atcgtcgatc gcgcgatttg caatcacatg
     3001 atcgcgatcg acatcctgcg gtgagattgc gtcaacctcg ccgcctgtcg tcacattgat
     3061 gccggagtcg acgagtcgct tcacccgtcg cacacctgag cgattagcga gcagtccatt
     3121 ccacagctcg tgcgtcgttg cgccgagcgc cgagagcgcg cctgtgccgg tgatgactac
     3181 gcgccgcttg tctgacatta cgagacttcc agattaaccg tgacgcctga aaaaccgtct
     3241 gtgtgttcgg ccggtacaac gtacgacata ggcccacgat tgcattgcgc a
//
LOCUS       DASSFQ010000025         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2096629, whole genome shotgun sequence.
ACCESSION   DASSFQ010000025 DASSFQ010000000
VERSION     DASSFQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096629"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VFH96_00760"
     CDS             <1..1629
                     /locus_tag="VFH96_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6972517.1"
                     /translation="GLRSSAFNFRLASMKKCPKCGVEYPDTTTLCPADGIALEKDPDS
                     LVGATLAGKYRIDARLNEGGMGTVYRGTHVLMDKTVAIKVLRPSLAADEKIVARFSRE
                     ARAASRISHPNAISVTDFGEDESGHVFLVMEFLSGKTLKQLIRDEGPLPLARVVDITR
                     QVADALNAAHEQGVVHRDLKSDNIMLVDTMGGDHAKVLDFGIAKINEPDGNPVDTGLT
                     APNLVIGTPQYMSPEQCSQDGEIDARSDIYSLGVILFEMLVGHVPFSGDSPTMVMMKH
                     LQEPVPSVLEERSDLPASVARVVARAMAKVRDNRYQTVAELIEDLTIAAGMTIHRIGP
                     VTATNTGPTAPVQDDEVTMVRPRAEAPYVPPQPVVRREPVRVPVSGGMPPPQSASSFN
                     PLKIIIPSLVGLLVVFAAVFALTRNSGAGEANTNQQNQTLAADPNSQPVQPGQSPSGK
                     GEAGIPSGGTIVPPANVNASPNANAAVSPAAIVDASPAANANANENSNSNGNSNRKAP
                     ALPEPTKSVLPDTPPPPAPAASKPAEKPSPTATP"
     gene            complement(1727..2755)
                     /gene="lpxK"
                     /locus_tag="VFH96_00765"
     CDS             complement(1727..2755)
                     /gene="lpxK"
                     /locus_tag="VFH96_00765"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET6972518.1"
                     /translation="MKSIVLPPLSLLYGAVTRTRLSLYRRGTFQTTKLDRPVISIGNI
                     TTGGTGKTPLVEYVARLIAAQGKKVCILTRGYGRKDPHLQVIVSDGYGVLVSPSEAGD
                     EPYLLATRLKGLAAVISSADRIAAGREAIKDFGTECFVLDDGFQHLRIARDLNIVTID
                     ATNPWGGGRLLPHGRLRESLDGLRRADCVVITRCDQVDGVGSLREEISRLTNGRPVFE
                     SRMEMGRVSPLKNGGEVLAAPARVAAFCGVGNPGAFFAQLRQAGYEVVVEKAFHDHHV
                     YSQEEVEAVVKAAKEAQADALITTAKDAVKLRALSFSVPCYVMEIEIEIENGDEFTKL
                     VLTAASGE"
     gene            complement(2752..>3012)
                     /locus_tag="VFH96_00770"
     CDS             complement(2752..>3012)
                     /locus_tag="VFH96_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972519.1"
                     /translation="GVAIVTGAHTHNFQAIVALMNEANALIQLPPLGCPVGEELADAF
                     AKLLANTDERAELGRRAKQLVTDNQGAADRTIELISPLLATS"
BASE COUNT          670 a          892 c          829 g          621 t
ORIGIN      
        1 ggtttgcgtt catccgcgtt caactttaga ctggcttcaa tgaagaaatg tccaaaatgc
       61 ggagtcgagt atcctgacac gacgacgctt tgtcccgctg acggtattgc gctcgagaag
      121 gatcctgact cacttgtcgg cgccacactc gccggaaaat atcgcatcga cgcacgactc
      181 aacgaaggcg gcatgggaac tgtctatcgc ggcacacacg tgctgatgga caagaccgtt
      241 gccatcaagg tgctgcgtcc atcgttggct gccgatgaaa agatcgtcgc gcgattctca
      301 cgcgaagcgc gcgcagcttc tcgtatctca catccaaacg caataagtgt tactgacttc
      361 ggcgaggacg agagtggaca cgtcttcctc gtgatggaat ttctgagtgg caaaactctc
      421 aagcagttga tccgcgacga aggcccgttg ccgctggcgc gcgtggtcga tatcacgcgc
      481 caggttgctg atgcgctcaa cgctgcacac gaacagggcg ttgttcatcg cgatctcaag
      541 agcgacaaca tcatgctcgt cgatacgatg ggcggtgacc acgccaaggt gctggacttc
      601 ggcattgcca agatcaacga gccggatgga aatcccgtcg acactggttt gactgcgcct
      661 aacctcgtca tcggcacacc gcaatacatg tcgccggagc agtgttcgca ggacggggag
      721 attgatgcgc gttcggatat ctactcgctc ggtgtgattt tgttcgagat gctggtcgga
      781 cacgttccgt tttcaggcga ctcgccgacg atggtgatga tgaagcactt gcaggagcca
      841 gtgccttcag tgctcgaaga gcgatcggat ctaccggcgt cggtggcgcg agtggttgcg
      901 cgtgcgatgg cgaaggttcg tgacaatcgt tatcaaaccg tcgccgaatt aatcgaggat
      961 ttgacgatcg cagctggtat gacgatccac cggattgggc cagtaactgc gaccaatacc
     1021 ggaccaactg cgccagtaca ggacgacgag gtgacgatgg tgcggccgcg cgcggaagcg
     1081 ccgtacgtgc caccgcagcc ggttgtgcgg cgcgaacctg tcagagttcc tgtttcgggt
     1141 gggatgccgc cgccgcagtc tgcgtcgagt ttcaatccgt taaagatcat cattccatcg
     1201 ctggtggggt tgctagtggt gtttgcggcg gtttttgcgt tgacgaggaa ttcgggcgcc
     1261 ggtgaggcga acacgaatca gcagaatcag acgctcgcgg cagatccgaa cagtcagccg
     1321 gtgcagccgg ggcagtcgcc gagtggcaag ggtgaagcgg gaattccgtc gggtggaacg
     1381 attgtgccgc cagcgaatgt gaatgccagt ccgaatgcga atgcggcggt gtcgccggca
     1441 gcgattgtgg atgctagtcc ggcggcgaat gcaaacgcga acgagaacag taattcaaac
     1501 ggtaacagca atcgcaaagc acccgcattg cctgaaccga cgaagagcgt tttgccggat
     1561 acgccgccac cgccagcgcc agcggcgagt aagccggccg agaaaccctc accaaccgcg
     1621 acgccataga gatcgtttca cgcaaaggcg caaagaaagc aaaggcgcaa agaatggatt
     1681 caactcttct ttgcgccttt gcttcctttg cgcctttgcg tgaaacttac tctccgctag
     1741 cagccgtcaa gaccaatttc gtgaactcat cgccattttc gatctcgatc tcgatttcca
     1801 tcacataaca cggaaccgag aacgacaacg cccgcagctt cacggcatct tttgctgtcg
     1861 ttattaatgc atcggcctgc gcctctttcg ctgcctttac aaccgcctca acctcttcct
     1921 gcgaatacac atgatgatcg tgaaacgcct tttccaccac gacctcatac cccgcctgcc
     1981 tcaactgcgc aaagaacgcc cccggattgc caactccgca aaacgccgcc acacgcgccg
     2041 gcgccgcgag cacctcgcca ccattcttca acggcgacac gcgccccatc tccatccgcg
     2101 actcaaaaac cggtcgtcca ttcgtgagcc gcgaaatctc ttcccgcaaa ctgccgaccc
     2161 catcaacctg atcgcatctc gtaatcacca cacaatctgc tcttcgcaaa ccatcgagcg
     2221 actctcgcaa gcggccgtgc ggcaatagtc gcccaccgcc ccacggattc gtcgcatcaa
     2281 tcgtcacaat gttcaaatcg cgcgcgattc gcagatgctg aaagccgtca tcgagcacaa
     2341 aacactccgt gccaaaatcc ttaatcgctt cgcgtcccgc cgcgatccga tcggcgctac
     2401 tgatcaccgc cgccagacct ttcaatcgcg tcgccaaaag atacggctca tcacccgctt
     2461 cgcttggtga aacaagcacg ccatatccat ccgaaacgat cacctgaagg tgtggatcct
     2521 tgcgtccata gccacgcgtc aggatgcaaa ccttcttgcc ttgtgcggca atcagcctcg
     2581 cgacgtattc aacgagaggg gttttgccgg tgccgccggt ggtgatgtta ccgatgctga
     2641 tcactggacg atcgagcttc gtggtttgaa aagttccgcg acgatacaac gagagacgag
     2701 tgcgcgtgac ggcgccgtac aagagactca atggtggcag aacgattgac ttcatgaagt
     2761 cgccagaagt ggtgaaatga gttcgatagt gcggtcagcc gcgccctggt tatcggtgac
     2821 gagttgtttg gcccgccgac ccagttctgc gcgctcgtct gtgtttgcca gcagtttagc
     2881 gaaagcatct gcgagctctt cgccaacagg gcagcccaaa ggcggcaact gaattaatgc
     2941 gtttgcttcg ttcattagcg caacgatggc ctggaagtta tgcgtgtgcg caccggtcac
     3001 gatcgctact cc
//
LOCUS       DASSFQ010000026         5241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1501884, whole genome shotgun sequence.
ACCESSION   DASSFQ010000026 DASSFQ010000000
VERSION     DASSFQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501884"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1824
                     /locus_tag="VFH96_00775"
     CDS             <1..1824
                     /locus_tag="VFH96_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6972520.1"
                     /translation="LKSVLKEIENAQGAIILFIDELHTLVGAGAAEGAIDASNMLKPA
                     LARGELRCVGATTLAEYQKHIEKDAALERRFQQVYVGEPTVEDTIAILRGLKERYEVH
                     HGVRIKDSAIVAAATLSNRYITDRFLPDKAIDLIDEAASRLRIEIDSLPQEIDQLERE
                     IIQLEIERQALQREEDEKSKARLHEIEQRIADLKETSAGMKAKWQSEKEAIDHIRSAK
                     AELEQLRFQLDQARNNGELGRASEIQYGKIPELEQKLKGDQDKLANFQQDGVMLKEEV
                     DEEDVAEVVAKWTGIPVSRMLEGEMQKLVTMEGRLSNRVIGQEEALAAVANAVRRARA
                     GLQDPNRPIGSFIFLGPTGVGKTETARALAEFMFDDERAMVRLDMSEYMEKHAVARMI
                     GAPPGYVGYDEGGQLTEAVRRRPYSVILFDEIEKAHPDVFNVLLQILDDGRLTDSKGR
                     TVDFKNTVLIMTSNLGSREIQAAEGDEKQVREAVIQELRLHFKPEFLNRIDDIVVFHQ
                     LSRDQIGKIIDVQLERLTAMLHERNVTLVLEPSARELLMREGYDPTYGARPLKRAIQS
                     LIQNPLAVKLLQGEIQPGQTVRVSANGDVMEFKTDAAFATA"
     gene            1875..2285
                     /locus_tag="VFH96_00780"
     CDS             1875..2285
                     /locus_tag="VFH96_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6972521.1"
                     /translation="MLQDRMNRLFEDATQRRSQPDGGAGDEFERADWMPASDIYETDS
                     GYMIAMDLPGIDREALVIDIDDNRLVVKGTRAITESRASRTERPRGKFLRTYSVPGSV
                     EQGKIAAEYKDGVLQINLPKRNEQKPKKIDIKIS"
     gene            2299..2913
                     /locus_tag="VFH96_00785"
     CDS             2299..2913
                     /locus_tag="VFH96_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6972522.1"
                     /translation="MTNEENELRVTDRRRVYLDDQGTERATTETEQPSLKPSYVEELE
                     TRTKAAERQVLEVQARFDQLRQQLQRETDETRQRLNRSADERAAGEKAKFIASVLPAL
                     DNLNRAAASGDAPRETILEGIRSIATTFEAALTNAGVEPIQSVGEEFNPELHEAVDTE
                     ETGSEMDGKVIEEYSRGFRMGDRLLRPARVKVGRATEQSTKKSV"
     gene            2944..4575
                     /gene="groL"
                     /locus_tag="VFH96_00790"
     CDS             2944..4575
                     /gene="groL"
                     /locus_tag="VFH96_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6972523.1"
                     /translation="MPAKELKFREEARNAMLQGVDVLANAVRVTLGPKGRNVVLGKKF
                     GAPVITKDGVTVAKEIELKNKYENMGAQMVREVAVKTNDKAGDGTTTATVLAQAIFRE
                     GVKNVAAGANPMSLQRGIQLATDQVVAELERMSKKIKSKEELTNVATVSANNDRHIGE
                     LISEAMDKVGKDGVVTVEESRTLQTELDIVEGMQFDRGFLSPYFVTNSDRMEAILDEP
                     YVLLYDKKISAMRDLLPLLEQTVGAGRALLIIAEDIDGDALATLVVNRLRGTIKVCPV
                     KAPAFGDRRKAILEDLAILTGGRVITEDLGIKLENVTIEDLGTAKRVIVDKETTTIVE
                     GGGDSKAIQGRVAMIRRQIEETTSEYDKEKLQERLAKLAGGVAVVKVGAPTEMAMKEV
                     KMRVEDALNATKAAAQEGIVVGGGVALLRAAKVLENHDHADPDVRTGVKIVQRALQEP
                     LRRIAENAGTEGAVVVGEVEQLKGNKGYNAVTGEIVDLAAAGIIDPTKVVRTALQNAA
                     SIAGLLLTTEAAVTDAPEKRKAGPPMGGGGDDYDY"
     gene            4825..>5241
                     /locus_tag="VFH96_00795"
     CDS             4825..>5241
                     /locus_tag="VFH96_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET6972524.1"
                     /translation="MVDLGSYKDKFGDSGQRILEHALNESRRRDQNFVSVEHILQALT
                     VEEPDMFNSTMRELSLDPVVVKMAIEKRLTNSRQSHVGKGFRIGPDTTDLFKRAMERA
                     RLQGRKTIEATDIFEALSNHDSLFVDVLRNMGASPEA"
BASE COUNT         1380 a         1304 c         1556 g         1001 t
ORIGIN      
        1 ttgaaatccg ttctgaaaga gattgagaac gctcaaggcg cgatcattct tttcatcgac
       61 gagctgcaca cactcgtcgg cgcgggcgct gccgaaggcg cgatcgacgc gtcgaacatg
      121 ttgaagcctg cgcttgcgcg cggcgagctg cgttgcgttg gcgccaccac actcgctgaa
      181 taccagaaac atatcgagaa agatgcggcg ctcgaacgtc gcttccaaca ggtttacgtc
      241 ggtgaaccga ctgtcgaaga cacgatcgcg atcctgcgtg gcttgaaaga gcgttacgaa
      301 gttcatcacg gcgttcgtat caaagactca gccatcgtcg ccgccgcgac tctttcaaat
      361 cgttacatca cggatcgttt tctgcctgac aaagcgatcg atttaattga tgaagcggcg
      421 tcgcgattaa gaatcgagat cgattcactc ccgcaggaga tcgatcaact cgagcgtgag
      481 atcatccagc tcgagatcga gcgccaggcg ttgcaacgag aagaagacga gaagtcgaaa
      541 gcgcgtttgc acgagatcga acaacgcatt gccgatctga aggaaacttc ggcgggaatg
      601 aaggccaagt ggcaatctga aaaagaagcc atcgatcaca ttcgctccgc taaagccgag
      661 ctcgaacagc ttcgcttcca gctcgaccag gcacgcaaca acggcgagct cggtcgcgct
      721 tctgagatcc aatacggcaa gattcccgag ctcgagcaaa agctgaaagg cgaccaggac
      781 aaactcgcga acttccaaca ggacggcgtc atgctgaagg aagaagttga cgaagaagac
      841 gtcgcggaag tcgtcgccaa gtggaccgga attccggttt cgcgaatgct cgaaggcgag
      901 atgcagaagc tcgtcacgat ggaaggtcga ctcagcaatc gcgtgatcgg acaggaagaa
      961 gcactcgcgg cagtcgctaa cgctgtcagg cgtgcgcgtg cgggcttgca ggatccgaac
     1021 cgcccgatcg gctcgttcat ctttcttggt ccaaccggcg ttggtaaaac tgaaactgcc
     1081 cgcgccctcg cggaattcat gttcgacgat gagcgcgcga tggtccggct cgacatgtca
     1141 gagtacatgg agaaacacgc ggtcgcgcgc atgatcggag cgccgccggg atatgttggt
     1201 tacgatgaag gtggacagct tacggaagca gttcgtcgcc gtccgtactc agtaatcctc
     1261 ttcgacgaga tcgaaaaggc gcatccggac gtttttaacg tgctgctcca gattcttgac
     1321 gacggcagac tgacggactc gaaaggtcga acagtcgatt tcaagaacac tgttctgatc
     1381 atgacttcaa atctcggctc gcgtgagatc caggcggccg aaggtgacga gaagcaagtg
     1441 cgtgaagctg tcattcagga gctgcggttg catttcaaac cggagtttct gaatcgtatc
     1501 gacgacatcg tcgtctttca ccagctctcg cgcgatcaga tcggcaagat catcgatgtg
     1561 caattggagc gtctgacggc gatgttgcac gagcgcaatg tgacgctcgt gttggagcca
     1621 tcggcgagag agcttttgat gcgcgaaggt tacgacccga cgtacggtgc tcgaccattg
     1681 aagcgcgcga ttcagtcgct gattcaaaac ccgctagcgg taaaactctt gcagggcgag
     1741 atacagcctg gccaaaccgt gagagtgtca gcgaatggcg atgtgatgga gtttaagact
     1801 gacgcggcgt ttgcgaccgc ttgaggagct ttgaaaacat gtcacaacag tggaaccctt
     1861 tgcaggacct ggtaatgctg caagatcgaa tgaacagact tttcgaagac gccacccaaa
     1921 ggcgcagcca gccggatgga ggcgcaggcg atgagtttga gcgagcggat tggatgcctg
     1981 cttccgacat ctatgaaact gattcgggct acatgatcgc gatggacctg ccgggaatcg
     2041 atcgcgaggc tttagtaatc gacattgacg acaaccggct cgtggtaaaa ggcacgcgcg
     2101 ctattacaga gtcgcgggcg tctcgcaccg agagaccgag aggaaagttt ttgcgaacgt
     2161 acagtgttcc gggatcggtc gagcaaggca agatcgcggc ggagtacaaa gacggagttt
     2221 tgcagatcaa tctgccgaag cgaaatgaac agaaaccgaa gaagattgat atcaagatta
     2281 gttagggaca gacggactat gactaacgaa gagaacgagc taagggtgac ggatcgtcgt
     2341 cgcgtttacc tcgacgatca gggaactgaa cgcgcaacca cggaaaccga acagccaagt
     2401 ctcaagccga gttacgtcga agagctcgaa acacgcacga aggctgctga acgacaggtg
     2461 ctggaggtcc aggcacgttt cgatcagttg cggcaacaac tgcaacgcga gactgacgaa
     2521 acgcggcagc gattgaatcg ttcggctgat gagcgcgccg cgggtgagaa ggcgaagttc
     2581 atcgcgtcgg tgctgcccgc tctcgataac ttgaacagag cggccgcgtc cggagatgca
     2641 cctcgcgaaa cgatcctcga aggcattcgc agtatcgcga cgaccttcga agcggcactg
     2701 acgaatgccg gagtcgaacc gatccagtct gtcggcgaag agttcaatcc cgagctgcac
     2761 gaagctgtgg acactgaaga aacgggcagc gagatggacg gaaaggtaat cgaggaatat
     2821 agtcgcggtt tccgaatggg cgatcgtctg ctgcgaccag cgcgcgtgaa agtcggacgc
     2881 gcaactgagc aatccacaaa gaagtctgtg taatctgtgg ctaatactta aggagaaaac
     2941 agagtgccgg caaaagagtt gaagtttaga gaagaagcac gcaacgcgat gcttcagggc
     3001 gtagacgtgc ttgccaacgc agtgcgcgtg acgcttggac cgaaaggtcg caacgtggtt
     3061 ttgggaaaga agtttggcgc gccagtgatc accaaagacg gtgtcacggt cgcaaaagag
     3121 atcgagctca agaacaagta tgaaaacatg ggcgcgcaga tggtccgcga agtcgccgtc
     3181 aaaaccaacg acaaagcagg cgacggcacg accaccgcga cagtcctggc gcaagctatc
     3241 tttcgcgaag gcgtgaagaa cgtggctgcc ggtgcgaacc cgatgtcttt gcagcgtggt
     3301 attcagctag cgaccgatca agttgtcgcg gagctggagc gcatgtcgaa gaagatcaag
     3361 agcaaagaag agctgacaaa cgtcgccact gtttctgcaa acaacgatcg tcatatcgga
     3421 gaactgatct ccgaagcgat ggacaaagtc ggaaaagacg gcgtggttac ggtcgaagaa
     3481 tcaaggacac tgcaaaccga gctcgatatc gtcgagggca tgcaattcga tcgcggtttc
     3541 ctgtcgccct acttcgtcac gaactccgat cgcatggaag caattttaga cgagccatac
     3601 gttcttcttt acgacaagaa gatctccgcg atgcgcgatc tgctgccgct gctcgaacag
     3661 acagtcggcg ccggacgtgc actgctgatc atcgctgaag atattgacgg tgacgcgctc
     3721 gcaacgctcg ttgtgaatcg cctgcgtgga acgatcaaag tttgtccagt gaaagcgcct
     3781 gcgtttggcg atcgacgcaa agcgatcctc gaagatctcg cgatcctgac gggtggcaga
     3841 gtcatcactg aagatctcgg catcaagctg gagaacgtga cgatcgagga tctcggaact
     3901 gccaaacgtg tgattgtcga taaagagact acgacgatcg tcgaaggcgg cggcgacagc
     3961 aaagcgattc agggtcgggt ggccatgatt cgcagacaga tcgaagagac aacgtctgaa
     4021 tacgataaag aaaagcttca ggaacgactc gcaaagctgg cgggcggcgt tgcggtagta
     4081 aaagttggtg cgccgaccga gatggcgatg aaagaagtca agatgcgtgt tgaagacgca
     4141 ttgaacgcga ccaaggctgc agcgcaggaa ggaatcgttg tcggaggcgg cgtcgcgttg
     4201 ctgcgagcgg cgaaggtgct tgaaaaccac gaccacgcag atccggacgt tcgcacgggc
     4261 gtgaaaatcg tgcagcgcgc gttacaggag ccgctgcgaa gaattgccga aaacgccggc
     4321 actgaaggcg cggtggtcgt cggtgaagtc gaacagttga agggaaacaa aggctacaac
     4381 gcagtcactg gcgaaatcgt agatctcgcc gcggcgggaa tcatcgatcc aaccaaagtt
     4441 gtacgcacag cgctacaaaa cgctgcgtcg atcgctggat tgttacttac cactgaagct
     4501 gctgtgaccg acgcgccgga gaagaggaaa gctggcccgc cgatgggcgg cggtggcgac
     4561 gattacgatt actagaactt catccacaga ttacacagat tgatcctaaa aaaatctgtg
     4621 caatctgtgg atggttttcg ttcgaacggc agcaaatttg caacagagcg ctcgccagga
     4681 ttgttattgc attgaaacgc aacggcgctt atgctggcga catccaagtc cagcaacttg
     4741 gatagggccg agttgtttga aagttgagtg tatcgtcgca cttaattgct gatcaaaagg
     4801 agtacgacgg tcgtaaaaag agatatggtt gatttaggtt catacaaaga taaatttggc
     4861 gacagcggtc agcgcattct cgaacatgcg ttgaatgaat cgcgacgtcg cgaccagaat
     4921 ttcgtatctg tggagcacat cctgcaggcc ttaactgttg aggagcctga catgttcaac
     4981 tccacgatgc gtgagttatc gcttgacccg gtcgtggtca aaatggcaat tgagaaacgc
     5041 ctgaccaaca gccggcaatc acatgtcgga aaaggatttc gcatcggccc tgatacgact
     5101 gacctgttta agcgcgcaat ggaacgagcc cgattgcagg gccgcaagac gattgaagca
     5161 acagacatct ttgaagcatt gagcaatcat gacagtctgt tcgtagacgt actgcgaaac
     5221 atgggcgcca gccctgaagc t
//
LOCUS       DASSFQ010000027        19995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1952001, whole genome shotgun sequence.
ACCESSION   DASSFQ010000027 DASSFQ010000000
VERSION     DASSFQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19995
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952001"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..407
                     /locus_tag="VFH96_00800"
     CDS             12..407
                     /locus_tag="VFH96_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET6972525.1"
                     /translation="MNADLKYFKLTQKIIGVYFDVYNELGSGFLESVYQKSLALALES
                     AGLKVCSRIDIPVWFRGIQVGQFEGDLLVEQCVLLELKAARALGSAHQAQLLNYLRAT
                     EIEVGLLLNFGPKPEFKRVAYDNSRKRIR"
     gene            665..1378
                     /locus_tag="VFH96_00805"
     CDS             665..1378
                     /locus_tag="VFH96_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET6972526.1"
                     /translation="MPVTDSQTEHVSEIEAALLDAELFMKYQAPERAIKRLRTALEMS
                     GRSVKLRERLREICASYKQNEEAARQCLALASIYIEREDFDTAHDRLLEAKQLDGRIN
                     IAGGLEAIRRARRPDLKPVAAPEANFERRHFTLAGDLSTISVFDAIQVIENARLNGVL
                     TLSGEKHQGDVRFNDGRIVDAACGNSIGDPAFQKVVEITSGTFEFQKTAEQFPVRIQA
                     VSNTNLILDTLRMLDERNQ"
     gene            complement(1382..2143)
                     /locus_tag="VFH96_00810"
     CDS             complement(1382..2143)
                     /locus_tag="VFH96_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972527.1"
                     /translation="MSLEFALQYACPLRANYSERHLREWGRLSSLHAKLTTGDASAPS
                     IDKLEWVEKASPEIERMQEATIVCAHCPACLPLDTAGDGESVGCLGRINYPIEAQFEK
                     FLADRVQLSLDTLDPDDQPRLLRILIDPESPFDGEATKELRRVVTEDGLRFFELRVPI
                     VLTREAARLTTDNIFDLLAGFRSEDSDTTTYTRELPFEAAADYYDFLDLVLRSELSQS
                     EVARLTSRSRNYNQFLRLLAAIEKAEALNTRVLID"
     gene            2243..2413
                     /locus_tag="VFH96_00815"
     CDS             2243..2413
                     /locus_tag="VFH96_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET6972528.1"
                     /translation="MAVSQELLEILACPACKAKVELKPDGNALKCVECKRVYLIRDDI
                     PVMLIDEAIIEE"
     gene            2418..3545
                     /locus_tag="VFH96_00820"
     CDS             2418..3545
                     /locus_tag="VFH96_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6972529.1"
                     /translation="MNSLRDIVELSPQLRVIETQANYGLEAQPLAPARWDWSAVRKVL
                     LVRRRSIGDTVLATPSVFALKRFLPNATVDILVEDWVAPLLNNHPYVDNVIVLERGGF
                     MTRARVARELRAANYDVVYNLHGGTTATFLTRATGARHRVGFKSYQYAQLHNHQAPSP
                     LLLWGQQKTHSVEQQLALLGWTGVPVTDRPRTQLGISSESRAIVQTLLAESGLNDRKI
                     ALIHPAAAFATKQWATEHFARVAEFLSARGLAPVAIAAPNEQALLEKLCAEAAVKITT
                     LELSLPEVTALAAQSQLFVGNDSGIAHIAAAVGTPAVVIFGSSNIAHWRPWNSAPAEV
                     VFEEMPCQPCHGYFCEKFDQPECILRVPVTRVTAAIDRILS"
     gene            3549..4544
                     /gene="rfaE1"
                     /locus_tag="VFH96_00825"
     CDS             3549..4544
                     /gene="rfaE1"
                     /locus_tag="VFH96_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET6972530.1"
                     /translation="MPQLTKTRASEILKTLQDRYVLVLGDVMLDEFVWGDVTRISPEA
                     PVPVVDVRRESVHLGGAANVLANLVALGSRGSVVGVVGKDGPGERLQTELRGLGSQDG
                     CLVVDESRRSTTKTRIIAHSQLVVRADRESRVPVTSKIEDQIIACLKEALKQADAFVV
                     SDYDKGVVTPRILREILPVAYEQVPVLIDPKLRNFNEYRPATLVTPNHLEALRMSDSE
                     DHSDDGSHAAAKIIREKLDCDAVLITRGDRGMMLLEGDGGPVYVETAAREVYDVTGAG
                     DTVIAALACALACGATMVEAASFANRAAGIVVGKVGTATVSAEELIQTFAADEHG"
     gene            4551..6971
                     /locus_tag="VFH96_00830"
     CDS             4551..6971
                     /locus_tag="VFH96_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6972531.1"
                     /translation="MVGKTLRNYRITDKLGVGGQGAVYKATDTKLGRSVVIKVIPPEL
                     SAKQSNLKRFEREARLASSLDHPNICTIFDLDEVDGIHFIAMQFVEGKNVRQLVAGRP
                     LELKTALLIGLQVTDALAAAHSRGIIHRDIKSGNVMVTSTGQVKVLDFGLAKLLDDNE
                     ATTAGIHRTELTEVGVPYGTATYAAPEQARGDRVDKRADIFSLGVLLYEMLTGTWPFR
                     GKTTIDVRHAVLHDPPRPIAELRSSAIPPRLQQIIDKALEKEPKDRFQNMEDFRNELR
                     HVLHELDAGVTGYQSVTPEPPRHLAGANPVSRAVRWLKSITRTESPTSAPNVVSSSKS
                     GIHETPFTTVTDQEKKSLAILCFRNLSNDPAAAFYEFSLADAVITELARVRSLVVRPS
                     SVIARYQGQQRDPREIGQELAVSAVLTAGFIHSGDRFRVTAQLIDVATGELLWSDRID
                     TAASDIIVLQDTIAQRIVEGLSVELSPHEQVGTGKASTRNPAAYEQYLRGRDLFARFI
                     FRTLSAEDCDGAIEHFERAIQLDPNFALAHDGLGACYVNRVFKGFGGSEDYERAEAAF
                     TKALAIDPGIFEARMLMVFVYLWRGQKQKARDEVERTRREAPNEPVVHFVKALLHRLD
                     GEYGRALRSYDRLVRLDPAAHVVASYSRALVHMYMGHFDDTFRELNTSGDPDNALVRT
                     FRALALYYTGQTDEAAELMKDVVEKHPNMHGIRPFMAMFLSGQGKHDEALAQLTPDVK
                     RNGEVDADISYSIGSVYALEGLQGDAFEWLERSISLGNANRPCFENDPNLVSLRGDSR
                     FAELMEKL"
     gene            7006..8511
                     /gene="dacB"
                     /locus_tag="VFH96_00835"
     CDS             7006..8511
                     /gene="dacB"
                     /locus_tag="VFH96_00835"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET6972532.1"
                     /translation="MHKRKISLLAYLLLFCVSSAVAQQPKTVPELQTRIKEVLAKPEL
                     SSSMVGIKVVSLDNGRVLFEENATKLLRPASNMKLYTIAAALDRLSPDYRFSTSVYAN
                     TRPDAAGVVHGKLTIYGRGDPSIAARFNNGDYVKAIDDLATRIVAAGVKRVEGDIVGD
                     ESYFVGPKYGSGWNWEDLTWYYGAEITPLTVNDNALDLFIKPGRAIGQPALITTGPPD
                     PLLTIVNKVTTSAKGSKREFSIHRGLGENTITITGSIPLEDRGYTGGIGISHPGLLFV
                     YMLRSSLAQKGVTITGASRIDGLSTALENEIATLQSPPFSVVAAQTLKPSQNLYTELI
                     LRTLGKVSPPPPPSTTSNLPQTSEELGLEAVKTFLKTVGLRPEALVLDDGSGLSRNDM
                     ITADASVQLLTFMSRHRYATVFRDALPIGGVDGTLRNRFKGTPAENNVRAKTGSLSSA
                     ASLGGYMTTAGGEKLAFSIMVNNYPRDFDPRSSCIDPIAILLASFAGKATD"
     gene            8515..9375
                     /gene="dapF"
                     /locus_tag="VFH96_00840"
     CDS             8515..9375
                     /gene="dapF"
                     /locus_tag="VFH96_00840"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET6972533.1"
                     /translation="MGPQFTKFHGLGNDYLVIEADELREIGDLGEFARRICNRHYGAG
                     GDGIAIIAKVHTAEADFSCRIFNPDGSEAGLSGNGTRCAVAYLYYKQLWTKEELRLST
                     RTGLKRYFLRGQPGPGKFLFESELGQPEFDSKSIPMVTTPPLEKVINYPLPVNGESFP
                     VTAMQMGNPNCCIFVDDFNALDWRAIGKAIEVHPQFPDRTNVVFVRPVERDFIELRIW
                     ERGVGETTASGTCSCAGAVAAMVNDKADRDVRVLMEGGEVRIKWRPDGEVVITGTAEV
                     VYSGQWLQQL"
     gene            9407..10603
                     /locus_tag="VFH96_00845"
     CDS             9407..10603
                     /locus_tag="VFH96_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6972534.1"
                     /translation="MLKLSPIILALLLLSACGGKVITPTEAAKPPDPPKPPTSVPAWL
                     GNASRNFYGTGPWSGKKLEVVWEFETDWIKGRLHKDPWGGSSWPGQPSVDETRVYFGS
                     ADGRLYCLDKKDGRLIWSYKTEDSLKATPTIVGDRLISSGLDHYIYCIDARDGTLIWK
                     YKTGFEVDCSAAVINNRVYFGGEDGFFYSLNLDDGSLIYKTERLGSMEGSFSVVDDRA
                     YIGTEQGDLYSLNLSDGTTVWKARIGADSDSTPAVYEGKVYTAAENGVVYCFRQSDGE
                     LLWKYQAVGGTGKEKSGIWASPIVANGRVHIGSSNGYLYCLTADKGELVWRYKAKAAI
                     WGTSPLVDGRLIFGDKAGWIHAISADKGEAISELKIGDNINATPAILDGRIYIGAFNG
                     KLYCLK"
     gene            10603..11226
                     /locus_tag="VFH96_00850"
     CDS             10603..11226
                     /locus_tag="VFH96_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET6972535.1"
                     /translation="MLVYLVDNTVDGQGANPREIRAVLGRLIPGLEILTEPFHHVSLE
                     RVRSLRPSHIILSGQSHPWDQYSPESLAGVFDVIKHASQPILGVCGGHQQIAIAHGAE
                     VGLMGRLEPGEGYNGAKRERGFLAVENTGEGLFKGLPAEITVWHNHCDEVKELPDGFR
                     ATASNETCKIQAMQEKGRRVYGVQFHPELFDEEHPEGRQIVENFLKL"
     gene            11602..12075
                     /locus_tag="VFH96_00855"
     CDS             11602..12075
                     /locus_tag="VFH96_00855"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HET6972536.1"
                     /translation="MKVNYHFSGGAIIAVALLSFSLTFTATTLSAETLSSQEPQQPVV
                     SPKGSLGEQIAAEETYVMPPANYVATAYSLRGRTASGRPVAKGLIAADPRHLPLGSRV
                     RLEAGNYSGEYLVADTGSLVRGKRIDIWTPSSREAMRFGRRTVKLTVLSYGGRKK"
     gene            12201..13253
                     /locus_tag="VFH96_00860"
     CDS             12201..13253
                     /locus_tag="VFH96_00860"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET6972537.1"
                     /translation="MSQQIPLAEPLVEVRRGSITESRHRGHIVVVDTHGNIIASLGAP
                     ENVTYLRSSAKPFQAIPLLTTGAAEHFGFTDREVALACGSHNGEPIHTELAASMLRKI
                     GLGPEALRCGAHEPYGVQAALDLRVRGEEPSALHNNCSGKHAGMLAVALHLGASIDNY
                     ENPENPVQKAIVEVVSQFSGVAVTDMAIGVDGCAAPVFGITVKAMALAYARLIAPPET
                     FDKKTRDACERIVRVMSAYPELIGGTSDRLDTELMRAAPRRLVSKVGAEGVYTAGIKP
                     SEEWPQGLGIAIKIEDGDDKRARPTVVIESLRQLGVLRDESLEAVAKYAFFPVTNRRD
                     DVVGEIVASFDLKVKK"
     gene            13250..13774
                     /locus_tag="VFH96_00865"
     CDS             13250..13774
                     /locus_tag="VFH96_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972538.1"
                     /translation="MTSESDLQGPPLQRDAYGQVDVDSVPDVIEWFLNYDQRVAIIRH
                     PSVEEVFQWKQQHVQSQGEEIFQFNRAEDRLAIGIIQALADNSTERSLHEWIGQLLGA
                     LDTASKANETTADAYKLDLQSGGSIVKEAEKIPFQRGRTEFLLNCWIETLCTAEARVL
                     GWLYKEFYKGPYAP"
     gene            13823..14947
                     /locus_tag="VFH96_00870"
     CDS             13823..14947
                     /locus_tag="VFH96_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6972539.1"
                     /translation="MTPELRALFPITEHALYFNHAAVSPPPITAIRAVEAQLKDVHEN
                     GSINFRDWLAVKEQARELLAKLLGARSEQVAFVRNTSDALSSVANGLSWRNGDNIVTF
                     SREFPSNIYPWLRVREAFGVEVRMCEERDGRIDFAELSRLINHNTSVVAISHVQYASG
                     FRMDLERLGRLARRHDALFVVDVIQALGVVPTDVESEFIDVAAGAGHKWLLSPEGVGY
                     LYLSDRARDRIQPTLVGWISVPNPDDYFNFEQGWNRGALAWETGTGPASLLYGFKASI
                     ELLTRFGVQNVAKYLEELTDDLCERLRAKRYEVVSSRAPREKSQIVCVRHLDGLSAMQ
                     LYHQLSAKKIITAPRAGRLRIAPHFYNTPTEVEAFVNALP"
     gene            complement(15448..16182)
                     /locus_tag="VFH96_00875"
     CDS             complement(15448..16182)
                     /locus_tag="VFH96_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972540.1"
                     /translation="MSRSQSTPAPKGGIANDKTATSNDDDLRVLAKHLPPPEEAQKNA
                     VYLNSFTSLEKLGLQHPVFSAVQSGGDADAALGTYGPSKVLIIEFHTPQLASDNHQRI
                     IARIQELWRLGQPAPTAYRRVGNYSVFVFDAPDEQTAKQLIDQVKYEQVVQWLGDNPN
                     ILREAEKRYINTTLGVLVAVLKASGYAALLCLGVGGLIGGLLFTYRRSQQKAATAYSD
                     AGGMLRLNLDELSAETNPSRLLQDRN"
     gene            complement(16338..16919)
                     /locus_tag="VFH96_00880"
     CDS             complement(16338..16919)
                     /locus_tag="VFH96_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6972541.1"
                     /translation="MTCSSCGAYVPPSVRFCSNCGAPASDPEATRIARLQARSLEMSG
                     DQDLEHTVFTVRPTMMFVKAGYALAVVAGIALVFLLASLSVPASISIPIALALLLIPA
                     YYHIRRNMVRYTVTDSKLQIDTGLIARTTKNIPLSKVQDVTVSATIPQRLMGFGDIII
                     DNASELGGTIVMHNISKPRHYADLLLRELRRWH"
     gene            complement(17049..17756)
                     /locus_tag="VFH96_00885"
     CDS             complement(17049..17756)
                     /locus_tag="VFH96_00885"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6972542.1"
                     /translation="MRRTLYILAIFALAAGPAAAQYQVDNFDFPNAVKIQTPSKPATK
                     RLIQLTSMPLNPSLGGFTTGNSDVDNYIVESGKRNSVDPLLLYSIMHQESSFKPRAMS
                     NKGARGLMQLIPATASRFGVTNIWDPKQNIEGGARYMRFLLNLFNGDVRLALAGYNAG
                     EGAVMKYGYQVPPYSETREYVRRIGNRYSLIRDPEAIAQASSLPAGQNNPTPLNVYER
                     SVFMTRLPDGRLQLVSQ"
     gene            17917..18258
                     /locus_tag="VFH96_00890"
     CDS             17917..18258
                     /locus_tag="VFH96_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET6972543.1"
                     /translation="MKKILTLSLLLLCVFTVGVRAQNTTSTTAKSATKQGAKRGPIFR
                     ATKEQINQAQAILKERGFYGGEQLGKLDDATREGLRKYQQAEGLKVTGTLNKITLEKM
                     NINLTEKQKTM"
     gene            complement(18318..>19995)
                     /locus_tag="VFH96_00895"
     CDS             complement(18318..>19995)
                     /locus_tag="VFH96_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET6972544.1"
                     /translation="DFVVDFMKDVYPELETHREFVQKMVRLEEDRFRSTLTVGLNKLD
                     EVFATTKGAMPEYKTLARLYDTFGTPRDLIRVGLEERGFAIDEDEFNSAFDAALQELQ
                     QTTTSDKGETKAKANPVYANLAGRLSRSIFKGYETTNVTDAKVVAVIKGDEVESLSEG
                     EEGEIVLDHTPFYAESGGQVGDVGVIVGPTNELATVLDTYSPAQDLIVHKIKVEKGSF
                     KVGDTVSADVEVEKRNATRRNHTATHLMHAALREVLGTHVKQAGSVVAPTYLRFDFTH
                     YQPLTAAEKEEIERLVNYHILRNEPVQTDEMAIEEAMHSGAMALFGEKYGEQVRVLSI
                     KGVEGIFSKELCGGTHVRATGDIGLFKITSDESIASGVRRIRAITGMDAYERFREAEV
                     LVDGLSSGLRTSRNDLPLTVTRLQDELRKARRETDELKLKIASGAAGASSNGDESREV
                     AGVKVLAREASGLDAAAMRQLSDTLLARIKSGVVVLGRSSDGKVSLIVRTSADLTGKV
                     PAGQVIKELAPIIGGRGGGKADMAEGGGNQPENLQLALEKSFEVVKRLVS"
BASE COUNT         4429 a         5536 c         5730 g         4300 t
ORIGIN      
        1 gatgaacgcg aatgaacgcg gatctgaaat actttaagct gacgcagaag attattggtg
       61 tctactttga cgtttataac gagttaggga gcgggttctt agagtccgtt taccaaaaat
      121 ctcttgcgtt ggcgctcgaa tcggctggcc tgaaggtctg cagccgcata gatattcctg
      181 tgtggtttag aggtattcag gtcggccaat ttgagggaga tttactggtc gaacaatgcg
      241 ttcttcttga attgaaagcc gctcgtgcgc tgggctcagc tcaccaagcc cagctcctga
      301 attatttgag agctaccgag atagaggttg gtttgctgct caacttcggc ccaaagcctg
      361 aattcaagag ggtagcttac gacaacagca ggaagagaat tcgctgatcc gcgaaaatct
      421 gcgtttatcc gcggcttcta tacttttcgc accgagcccg tgagcgttcg attcttcttt
      481 tcatccaccg gttcattgta tttcgccaat cctttctgat gggggactac gatgagggtc
      541 cttcgcaggt tgcatgccaa tcgagtcttc ccagttgtaa cgcggattta gatgcagaaa
      601 aggaccatct agcgcaccat ttcgtgtcgc caggccgcaa atcgcttatg gtaaactcgc
      661 tccgatgccg gtcacggact cacaaactga gcacgtcagc gagatcgaag cagctttgct
      721 cgacgccgag ctcttcatga aatatcaggc cccggaacgg gccataaaac gcctccgaac
      781 ggccctggaa atgtccggcc ggtccgtcaa actgcgcgag cgcctgcggg aaatctgcgc
      841 cagttacaag caaaacgaag aggctgcccg ccagtgtctc gccctcgcca gcatctacat
      901 cgagcgcgag gactttgaca cggcccacga tcgcctgctg gaagccaaac agctcgatgg
      961 tcggataaac attgcaggcg gactcgaggc gatccgccgc gcccgccggc ccgatctgaa
     1021 acccgtagcc gcgcccgaag cgaattttga gcgccggcac ttcacgctcg cgggcgacct
     1081 cagcacgatc agcgtcttcg acgcgattca ggttatcgaa aacgcaagac taaacggcgt
     1141 actgacgttg agcggtgaaa agcaccaggg cgatgtgagg ttcaatgacg gacgaatcgt
     1201 cgatgcggcg tgcggtaata gcattggcga cccggcattt cagaaagtgg tcgagatcac
     1261 gagcggcacg ttcgaatttc aaaagaccgc agaacagttt cccgtccgca tccaggcagt
     1321 cagcaacaca aacctcatcc tcgacacgct ccgcatgtta gacgagcgca accagtaaga
     1381 cttaatcaat caaaacacgc gtattcaatg cttcggcttt ttcgatggcc gcgagcagcc
     1441 ggagaaactg gttgtagttt cgactgcgcg aagtgagtcg cgcgacttcc gactggctca
     1501 gctcgctgcg caaaaccaga tcgagaaagt cgtagtagtc ggcagcggct tcgaacggga
     1561 gctcgcgcgt gtaggtggtt gtgtcactgt cttccgatcg gaatccggcg agtagatcga
     1621 agatgttgtc ggtcgttagc cgggcggcct cgcgcgtgag aacgatcggc acgcgcaact
     1681 cgaaaaaccg caaaccgtct tccgtaacga cgcgccgcag ttccttcgtc gcttcaccat
     1741 caaacggcga ctccggatcg ataagaatac gcagcagtcg cggctgatcg tcgggatcga
     1801 gcgtgtcaag tgagagctgc acgcgatcgg cgagaaactt ttcaaactgc gcttcgatgg
     1861 ggtagttgat ccgaccaaga cagcccacgg attcaccgtc gccggcggta tcgagcggca
     1921 gacacgccgg gcagtgcgcg cagacgatcg tcgcttcctg catgcgctcg atttcagggc
     1981 tcgccttttc aacccattca agtttatcga tcgaaggcgc gctggcgtcg cccgtcgtga
     2041 gcttcgcgtg caagctgctg agccgtcccc actcgcgcaa gtgacgctcg gagtagttgg
     2101 cgcgaagggg gcaggcgtat tgcagtgcga attccaacga catgctctga agtataggtc
     2161 atatgagacg gataggtcat ataggtccta tactgctttg tgataacatt ttgtgcattc
     2221 cgcgcgaaag aaaggtttgg ccatggcagt cagtcaggaa ctcttggaaa tactcgcgtg
     2281 tccggcttgc aaggcgaaag ttgagctcaa gccggatgga aacgcgctga agtgcgtcga
     2341 gtgtaaacgc gtctatctca tccgcgacga cattccggta atgctaattg acgaggccat
     2401 catcgaagag tgatgcgatg aacagcctgc gcgacatcgt tgagctctct ccacagctac
     2461 gcgtcatcga aacacaggca aactacggat tggaggcgca gccgttggcg cccgcgcgct
     2521 gggactggtc agccgtgcgc aaggtgctgc tcgtccggcg gcgatcgatt ggcgacacgg
     2581 ttttggccac accttcagtc ttcgctttaa aacgatttct tccgaacgcc acggtcgaca
     2641 ttctggtcga agattgggtg gcgccgctgc taaacaatca tccgtacgtc gacaacgtga
     2701 tcgtgttgga gcgcggcggg ttcatgacaa gggcgcgtgt ggcgcgtgag ctacgggcgg
     2761 cgaattacga tgttgtttat aacctgcacg gcggaacgac agcgacgttt ttgacgcgag
     2821 caactggcgc gcgtcatcgc gtcgggttca agtcgtatca atacgcacaa cttcataatc
     2881 accaggcgcc gtcgcccttg ttgctctggg gacagcagaa gacacactcg gtcgagcaac
     2941 aactcgcgtt gttgggttgg accggcgttc ccgtgacgga tcgtccgcga acccagttgg
     3001 gtatttcgag tgaatcgcgc gcaatcgttc aaacactact cgctgagtcc ggtctaaacg
     3061 atcgaaagat cgcgctgatt catcccgcag ccgcattcgc aaccaagcaa tgggccacgg
     3121 aacacttcgc gcgcgtcgct gagtttctgt ccgcgcgcgg cctagctcca gtcgcgattg
     3181 cagcaccgaa cgaacaagcg ctgctcgaaa aactctgcgc ggaagctgcc gtgaaaatca
     3241 cgaccctgga actctcgctg cccgaagtga ccgcactcgc cgcccaatcg caactgttcg
     3301 tcggcaacga tagcggcatc gcgcacatcg cagcggctgt cggaacacct gcggtcgtga
     3361 tctttggctc ttcaaacatc gcgcactggc gcccgtggaa ttcggcgccg gccgaagtcg
     3421 tctttgaaga gatgccgtgc cagccttgcc acggttattt ttgcgagaag ttcgatcagc
     3481 ccgaatgtat cctgcgcgtg ccggtgacga gggtcacggc cgcaatcgat cgcattttgt
     3541 cgtaacgcat gcctcaatta acaaaaactc gtgcttcgga aatcctgaag acacttcagg
     3601 accgttacgt tctggtgctg ggcgacgtga tgctcgacga gtttgtttgg ggcgacgtca
     3661 cgcgcatctc tcccgaagcg ccggttccgg tcgtcgatgt gcgtcgcgaa tcggtgcatc
     3721 tcggcggcgc tgcgaacgtg ctcgcaaacc tcgtcgcgct cggctcgcgc ggatcggtgg
     3781 tgggcgtggt gggcaaagat ggaccaggcg agcgtttgca aactgagctg cgcgggctcg
     3841 gatctcaaga tggttgtttg gtggtggatg agtctcgtcg ctcgacaaca aaaacgcgca
     3901 tcatcgccca cagccagctc gtcgtgcgcg ccgatcgcga gtcgcgcgtt cccgtcacgt
     3961 cgaaaatcga agatcagatc atcgcgtgcc tgaaagaagc gctgaagcag gcagacgcgt
     4021 ttgtagtttc agactatgac aaaggtgtag tgacgccgcg catcctgcgt gaaatcttgc
     4081 ccgttgcata cgaacaggtg ccggtgctga tcgatccgaa gctgcgcaac ttcaacgaat
     4141 atcgcccggc gacgctggtc actccgaatc atctcgaagc attgcgcatg agcgacagcg
     4201 aagatcattc cgacgacggc tcgcacgcgg cggcaaaaat aattcgcgag aagcttgatt
     4261 gtgatgcggt gttaataaca cgcggcgatc gtggaatgat gttgctcgaa ggggacggcg
     4321 gacctgttta cgtcgaaaca gcggcgcgcg aggtctatga tgtcacgggc gctggcgata
     4381 ccgtcatcgc cgcacttgcg tgtgcacttg catgtggggc gacgatggtt gaggcggcgt
     4441 cgttcgcgaa tcgcgccgcc gggatcgtgg ttgggaaggt cggaaccgca actgtcagtg
     4501 cagaagaact aatacagact tttgccgcgg atgaacacgg ataaacgcgg atggtaggta
     4561 agacgctacg aaactatcga ataactgaca agctcggtgt tggcgggcag ggcgcggttt
     4621 acaaagccac cgacacgaag cttggccgct cggttgtgat caaggtcata ccgccggagc
     4681 taagtgcgaa acaatcaaat ctcaagcgtt tcgaacgcga ggcacgcctc gcctcctcgt
     4741 tggaccaccc aaacatctgc acgatcttcg atctcgatga agtcgacggc attcacttca
     4801 tcgcgatgca gtttgtcgaa ggtaagaacg tgcggcagct cgtcgccggc cgtccactcg
     4861 aactgaaaac cgccctcctg atcggcttgc aagtgaccga cgcgctcgca gcggcacata
     4921 gccgcggcat cattcatcgc gatatcaaat ccggaaacgt gatggtgact tcgacgggcc
     4981 aggtaaaagt gctcgacttc ggactcgcca agctgctcga cgacaatgaa gccacaaccg
     5041 ccggaattca tcgcacggag ctcacggaag tcggcgttcc gtacggcacc gcgacttacg
     5101 cggcgccgga acaggcgcgc ggcgatcgcg tcgataagcg tgcagacatc ttttcactcg
     5161 gcgtgttgct ttacgagatg ctgaccggca cgtggccgtt tcgcggtaag acgacgatcg
     5221 acgtgcgtca cgcagtgctg cacgatccgc cgcgaccgat tgcagagttg cgctcaagcg
     5281 ccataccgcc gcgactgcaa cagatcatcg acaaagcact cgagaaagag ccgaaggatc
     5341 gtttccaaaa catggaagac tttcgcaacg agctgcgcca cgtgctgcat gaactcgatg
     5401 cgggcgtgac ggggtaccag agcgtgacgc ccgagccgcc gcgtcacctc gcgggcgcga
     5461 atcccgtttc gcgagccgtc cgatggttga aatcgatcac acgcactgag tcgccgactt
     5521 cagcgccaaa cgtggtcagt tcgtcgaagt caggcattca cgaaacgccg ttcacgacag
     5581 tcacggatca ggaaaagaag agcctcgcga ttctttgttt ccgaaacctg agcaacgatc
     5641 cggccgcggc gttctacgag ttctcgctcg ctgatgcggt gattaccgag ctggcgcgcg
     5701 tgcgttcgct agtcgtcagg ccttcttccg tgattgctcg ctaccaggga cagcagcgcg
     5761 atccgcgcga gatcggacag gagctcgccg tttctgccgt tctcacggcc ggctttattc
     5821 attccggcga tcgctttcgt gtgactgcgc aactgatcga cgtggcgacg ggcgagttgt
     5881 tgtggagcga tcgcatcgat acagccgctt cagacatcat cgtgctccag gacacgattg
     5941 cgcagcggat cgtcgagggc ttgagcgtcg agctgagccc gcacgaacag gtcggaaccg
     6001 gcaaagcatc aacacgcaat cccgcggcgt acgaacaata cctgcgcggt cgcgacttgt
     6061 tcgcgcgttt catcttccgc acgctgtcag cggaggattg cgacggagcg atcgagcatt
     6121 tcgagcgcgc catccagctc gatccaaact tcgcgcttgc tcacgacggc ctcggtgcgt
     6181 gctacgtcaa tcgcgtcttc aaaggtttcg gcggctcgga agattacgaa cgcgcggaag
     6241 ccgcgtttac gaaagcgctc gcaatcgatc cgggcatctt cgaagcgcgg atgctgatgg
     6301 tttttgtgta tctgtggcgc ggtcagaaac agaaggcgcg cgacgaagtc gaacgcacgc
     6361 gacgcgaggc gccaaacgag ccggtggtcc atttcgtcaa ggcgctgctg catcgtttgg
     6421 atggcgagta tggccgggca ctacgcagtt acgatcgttt agtacggctc gatccggcgg
     6481 cgcacgtcgt ggcgagttat agtcgtgcgc tcgtgcacat gtacatgggc catttcgacg
     6541 acacgtttcg cgagctgaat acgtcgggcg atcctgacaa cgcgctcgtg cgaacttttc
     6601 gcgcgcttgc gttgtactac acgggccaga ccgatgaggc agcggagttg atgaaagacg
     6661 tcgttgagaa gcatccgaac atgcacggga tacggccgtt catggcgatg tttctgagtg
     6721 ggcagggtaa gcatgacgaa gcgctcgcgc agcttacgcc ggatgtgaag cgcaatggcg
     6781 aagtggatgc agatatcagt tactcgatcg gatcggttta tgcgctcgaa ggattgcagg
     6841 gcgatgcgtt cgagtggctc gagcgatcga tctcgctggg gaatgcgaat cggccgtgtt
     6901 tcgagaatga tccgaatttg gtgtcgttgc gcggggactc gcggttcgct gagttgatgg
     6961 aaaaactttg aacgcggatt agacggattt aaaaccggat tttatatgca taaacggaaa
     7021 atctcgttac tggcctacct tctgctgttc tgtgtttcgt ctgcggtggc gcagcagccg
     7081 aagacagtcc cggaactaca aacccggatc aaagaggtac tcgcgaagcc ggagttgtcg
     7141 tcgtcgatgg ttggaatcaa ggtggtttca ctcgataacg gtcgcgtgtt gtttgaagag
     7201 aatgcgacga agctcctacg gccggcttcg aacatgaagc tctacacgat cgctgctgcg
     7261 ctcgatcgtt tgtctcccga ttatcgtttc tcgacttctg tttatgccaa cacacgtcct
     7321 gatgctgcgg gtgtcgtgca cgggaaattg acgatttacg ggcgcggcga tccgtcaatc
     7381 gcggcacgct ttaataacgg tgattacgtg aaggcaatcg acgatctcgc cacacgcatc
     7441 gttgcggcag gtgtcaagcg cgtcgaaggc gatattgttg gcgacgagag ttattttgtt
     7501 ggaccaaagt atggctcagg ttggaactgg gaagatctca cgtggtatta cggcgctgag
     7561 atcacaccgc taacggtcaa cgacaatgca ctcgatcttt ttatcaaacc cggtagggca
     7621 atcggccagc ccgcgttgat tacgactggt ccacccgatc cgttactcac gatcgtcaac
     7681 aaagtcacca cctcagcgaa aggcagtaag cgcgagttct cgatccatcg cggtctcggc
     7741 gagaatacga tcacgatcac cggcagtatt ccactcgaag atcgcggcta caccggcggc
     7801 atcggtatct cgcatcccgg tctgcttttt gtctacatgc tgcgttcgtc actcgcgcaa
     7861 aaaggcgtca cgatcaccgg tgcttcgcga atagatggct tgtcgactgc tcttgaaaac
     7921 gagattgcga cgttgcagtc accgccgttt agcgtggtcg cagcgcagac gctgaagccg
     7981 agtcaaaatc tctacactga attgatcctg cgaacgctcg ggaaagtctc accaccaccg
     8041 ccaccgtcaa ctacgtcgaa ccttccgcaa acgtctgaag agctcggtct cgaagcagtc
     8101 aaaacgtttc tgaagaccgt gggcctcagg ccggaagcgc tggtgctcga cgacggctcg
     8161 ggtctttcgc gcaacgacat gatcacggct gacgcgtcgg tgcagttgtt gacgtttatg
     8221 agcaggcacc ggtatgcgac cgtgtttcgc gatgcgttgc cgatcggtgg tgttgatgga
     8281 accttgcgca atcggttcaa aggcacgcct gctgagaata acgtgcgcgc caagaccggt
     8341 tcgctcagct cagccgcaag cctcggcggt tacatgacga cagcgggcgg cgagaagctc
     8401 gcgttttcga tcatggtcaa caactacccg cgcgatttcg atccacgatc ttcgtgtatc
     8461 gatccgatcg caatcctgct cgcctcgttc gcaggcaaag ccaccgatta acagatggga
     8521 ccacaattca ctaaattcca cggcctcgga aacgactatc tcgtgatcga ggcggatgag
     8581 cttcgtgaaa tcggcgatct cggtgagttt gcacgtcgca tctgtaatcg tcactacggc
     8641 gccggcggcg acgggatcgc gatcatcgcg aaagtgcaca cagccgaagc tgatttcagt
     8701 tgtcgcattt tcaatcctga cggtagtgaa gcggggcttt ccggtaacgg cactcgttgc
     8761 gccgtcgcgt atctttacta caaacaactc tggacgaaag aagagctgcg actcagcacg
     8821 cgaaccgggt tgaaacgcta cttcctacgc ggtcagccag gcccggggaa attcctattc
     8881 gaatcagaac tgggccaacc ggagttcgac agcaagtcga taccgatggt gaccacgccg
     8941 ccgctcgaga aggtcattaa ttatccattg ccggtgaacg gcgagagctt tccggtgact
     9001 gcgatgcaga tgggtaatcc aaactgctgc atcttcgtcg acgacttcaa cgcgctcgac
     9061 tggcgcgcga ttggtaaagc gatcgaggtg catccgcagt ttcccgatcg cacgaacgtc
     9121 gtgtttgtgc ggcccgtcga gcgcgacttt atcgagctgc ggatttggga gcgcggtgtc
     9181 ggcgagacga cggcttcagg cacctgctcg tgcgcgggcg cggtggcggc gatggtcaac
     9241 gacaaggccg atcgcgacgt gcgtgtgttg atggaaggcg gcgaagtccg gatcaagtgg
     9301 cggccggacg gcgaggttgt gattacggga actgctgaag tagtctattc cggccagtgg
     9361 ttgcagcagt tgtaggcagg attacacgat ttgcaggatt cagaagatgc taaaactctc
     9421 ccccatcatt ttagcgctgc tattgctctc cgcgtgtggc ggaaaggtca taaccccaac
     9481 cgaggcggcg aaaccaccag atcccccgaa accgccaacc tccgtgcccg cgtggctcgg
     9541 caacgcctcc cgtaacttct acggcaccgg cccgtggtca ggcaaaaagt tagaagtcgt
     9601 ttgggagttc gaaaccgatt ggataaaggg ccgtttgcac aaggatccgt ggggcggctc
     9661 gagctggcca ggtcaaccgt cagtcgacga aacgcgcgtt tacttcggct ccgctgacgg
     9721 tcgtctttat tgtctcgaca aaaaagacgg gcgactgatc tggagttaca aaactgaaga
     9781 cagtctcaaa gccacaccga caatcgtcgg cgatcgcttg atctccagcg gtctcgatca
     9841 ctacatctac tgcatcgacg cgcgtgacgg cacgctcatc tggaaataca aaaccggatt
     9901 tgaagtcgac tgctccgccg ccgtcatcaa caaccgcgtg tacttcggcg gcgaagacgg
     9961 tttcttctat agcctgaacc tcgacgacgg ctcgctgatc tacaaaacgg agcggctcgg
    10021 ttcgatggaa ggtagttttt cagtcgtcga cgatcgcgcc tacattggca ctgagcaagg
    10081 tgatctttac agtctcaacc tgagcgatgg aacaacggtc tggaaggcgc gcatcggcgc
    10141 cgattcagat tcgacgcccg cggtttacga aggcaaagtc tacacagccg ctgagaatgg
    10201 cgtcgtctat tgtttccggc agagcgatgg cgaactgctt tggaagtacc aggcggtcgg
    10261 tggaacgggc aaagagaaga gtggaatctg ggcgagcccg atcgtcgcga atggaagagt
    10321 acacatcggc tcgagtaacg gttacctgta ttgcctgaca gccgacaagg gcgagctcgt
    10381 ttggcgatac aaggcgaagg cagcgatctg gggaacgtcg ccgctcgtcg atggtcgttt
    10441 gatcttcggc gacaaagccg gctggattca tgcaatctca gcagacaagg gcgaggcgat
    10501 cagcgagttg aagatcggtg ataatatcaa cgcaacgccc gcgatactcg acggacgaat
    10561 ttacatcggc gctttcaatg ggaagcttta ctgtttgaag tagtgctcgt ttacctcgtc
    10621 gacaacacgg tggatggtca aggcgcaaac ccccgcgaga ttcgcgcggt gctcggtcgt
    10681 ctgatccccg gcctcgagat tctcaccgag ccttttcatc acgtctcact tgagagggtt
    10741 cgcagcctgc gtccttcgca catcattctc agcggtcaga gtcatccgtg ggatcagtat
    10801 tcaccggagt cgttggctgg agttttcgat gttatcaaac atgcgagtca gccgatcctg
    10861 ggtgtgtgtg gtggtcacca gcagatcgcg atcgcacacg gcgctgaagt cggactgatg
    10921 ggacgactcg aaccgggcga aggctacaac ggggcgaaac gtgagcgcgg tttcctggct
    10981 gtagaaaaca ccggcgaagg tctcttcaaa ggcttaccag cggagatcac tgtctggcac
    11041 aaccactgtg atgaagtgaa agagctgcct gatggttttc gcgcaaccgc atcgaacgag
    11101 acgtgcaaga tccaggcgat gcaggagaag ggcaggcgcg tttacggtgt ccagttccat
    11161 cccgagctgt ttgacgaaga acaccctgaa gggcgccaga tcgtcgaaaa cttcctcaag
    11221 ctctaaaccc ccttccgatt ggttacaatt caaaaacaaa cctctgtcga tgtgaccgta
    11281 tcgatttttc ttaacttttg atatttcact aattaccaac ggcgttgtta tttttctcac
    11341 aaaaatcacg gacttctcac ggttgaacgc accttaaacg ctgtggtagg gtcaacccga
    11401 atcgcggaca ttactggaag taaccgtccc ttttcgcttt atctagacaa gacacttcag
    11461 cgcaccgcat caaggatgca agcccccaac ctcggctgac tttatagcag actcctagag
    11521 ccaggggacg agtcatagga aagaacggac tcgcgcagga cgctgaagag taagtaacaa
    11581 catcggacag gagacccgaa tttgaaagtt aattatcact tcagcggagg tgcgatcatt
    11641 gctgttgcac ttctgagttt ctctttgact tttaccgcca ccactctctc ggcggagact
    11701 ctctcttctc aagaaccaca acaaccagtc gtttctccca agggtagctt aggagaacag
    11761 atcgcagccg aagagacgta cgttatgcct ccggcgaatt atgtcgccac tgcttacagt
    11821 ctgcgcggac ggactgcgag cggacgacct gttgccaagg gattgattgc tgccgatcca
    11881 agacacttgc ctttaggctc gcgcgtgcgg ctcgaagccg ggaattacag tggtgagtat
    11941 ctggtggcag acacaggcag tctcgtgcgg ggcaaacgca ttgatatctg gacaccatcg
    12001 tcgcgcgaag cgatgcgctt cggacgacgc acagtgaagt tgaccgtact cagctacggc
    12061 ggccgcaaga agtaacttcc ttctttagcc acaaaaggca caaaaagcac aaaaggtttt
    12121 gttgagtttg cttctcaatt aacctttgtg ctttttgtgc cttttgtggc tactgctctt
    12181 tgtggcaaga tgcagagcaa atgtcgcaac agataccgct cgcggagccg ctcgttgaag
    12241 ttagacgagg ctcgataaca gagtctcgtc atcgtggaca catcgtcgtt gtcgatacgc
    12301 acggcaacat catcgcgtcg ctcggcgcac ctgagaacgt cacttacctg agatcttcag
    12361 cgaagccgtt ccaggcaata ccgcttttaa ctaccggagc cgccgagcat tttggcttca
    12421 ccgatcgcga agtcgcgctc gcgtgcggct cccacaacgg cgaaccgatt cacaccgagc
    12481 ttgctgcatc gatgttgcgc aagatcggtc ttggtccgga agcattgcga tgcggcgcgc
    12541 acgagccgta cggcgtccag gctgctctcg atcttcgtgt ccgcggcgaa gaaccgagcg
    12601 cgctccataa caactgctcc ggcaaacacg ccggcatgct cgccgtcgcg cttcacctcg
    12661 gcgcgtcgat cgacaactac gagaatcccg agaatcccgt gcaaaaagcg atcgtggagg
    12721 tggtgtcgca attctcgggc gtagcggtga cggacatggc tatcggtgtt gacggttgtg
    12781 cagcgccggt gtttggaatc actgttaaag cgatggcgct cgcgtacgcg cgattaattg
    12841 cgccgccaga gaccttcgac aagaagacgc gcgatgcatg tgagcgaatc gtgcgcgtga
    12901 tgagcgcgta tccggagttg atcggcggaa cctcagaccg gctcgacacg gaactcatgc
    12961 gcgcggcgcc gcgtcgttta gtttcgaagg ttggcgcgga gggagtttac acggcgggca
    13021 tcaagccaag tgaagagtgg ccgcagggtt tgggaatcgc gatcaagatc gaagacggcg
    13081 acgacaagcg cgcgcgtccc acagtcgtga tcgagtcgtt gcggcaactg ggagtgttgc
    13141 gtgatgagtc gcttgaagca gtggcgaagt atgcgttctt tccggtgacg aaccgtcgtg
    13201 acgatgtcgt gggtgagatc gtggcttcgt tcgatttgaa agtgaagaaa tgacaagcga
    13261 aagtgatctg caaggtccgc cactgcaacg agacgcatac gggcaggtcg acgtcgattc
    13321 cgttccggat gtgatcgagt ggttcctcaa ctacgatcag cgagttgcga tcatcaggca
    13381 tccttcggtc gaagaggtat tccagtggaa acagcaacac gtgcagtcgc agggcgaaga
    13441 gatctttcaa ttcaatcgtg ctgaggatcg actcgcgatc gggatcattc aggctctggc
    13501 tgacaactca accgagcgca gtctgcacga gtggataggc cagcttcttg gcgcgctcga
    13561 tactgcgtcg aaagccaacg aaacaactgc ggacgcgtac aagttggact tgcaaagcgg
    13621 cggctcgatc gtgaaggagg cggagaagat ccccttccaa cgcggccgca ccgagtttct
    13681 gctgaactgc tggatcgaaa cgctctgcac cgcagaagct cgcgtactcg gatggctgta
    13741 taaagaattc tacaaaggtc cctacgcgcc ctgaatccgc cgttctgatc cgtgttatcc
    13801 gtggctaaaa acgtgaggtc gtatgacgcc tgagctgcga gcgttatttc ccatcaccga
    13861 acacgcgctc tacttcaacc acgccgccgt ctcgcctcca ccgatcacgg cgatccgcgc
    13921 cgtcgaagcg caactcaaag acgtccacga aaacggttca atcaacttcc gcgactggct
    13981 cgcagtaaaa gagcaggcgc gtgagctact cgcaaaactt ctcggcgcgc gatccgagca
    14041 agtcgccttt gtgcgcaaca cctccgacgc gctctcatcc gtcgccaacg gtctgagctg
    14101 gcgcaacggc gacaacatcg ttaccttcag tcgcgagttc ccgtcgaaca tctatccatg
    14161 gctgcgggtg cgcgaagctt tcggtgtcga ggtgcgcatg tgtgaagagc gcgacggccg
    14221 catcgatttc gcggagctct cgaggctgat taatcacaat acgagcgtcg tggctatcag
    14281 tcacgttcag tacgcctccg gctttcgcat ggatctggaa cgactcggcc gcctcgctcg
    14341 ccggcacgat gcattattcg tcgttgacgt gattcaggcg ctcggcgtcg ttccgaccga
    14401 tgttgaatcc gagtttatag acgtggcggc aggtgcaggc cacaagtggc tgctgtcgcc
    14461 ggaaggcgtc ggttacctgt atctctcgga ccgcgcccgc gatcggattc aacccacgct
    14521 cgtcggctgg atcagcgttc cgaacccgga tgactacttc aacttcgaac agggctggaa
    14581 ccgtggggcc ctggcctggg aaaccggaac cggacctgct tcgcttttat atggttttaa
    14641 ggcgagtatt gagcttctaa cgcgctttgg cgtgcagaac gtcgcgaagt atctcgaaga
    14701 gcttacagac gacttatgcg agcgtctgag ggcaaaacgg tacgaagtcg taagttcccg
    14761 agcgccccgt gagaaatctc agatcgtctg cgtccgccat ctggacggtt tatcagcgat
    14821 gcagctctat catcagctca gcgcgaagaa aatcatcacg gcgccacgtg ctggtcgcct
    14881 ccgaatcgcc ccacatttct acaacacacc gaccgaagtc gaagctttcg tcaatgcttt
    14941 gccttagacc gagcgattat cgttcgctgg tacagtggtg gttgtccgat agttgtacaa
    15001 atccgccaca catttgtgcg ctaacggaca atctctcgcc atttgaccac aaagaaattc
    15061 cagcaaaaac gccatttttg aatttgtccg tttcatatat ccctgttggc acggtcgttg
    15121 ccaaaagagg aaaacagaac aagaagggta gataagggta aatcgggtca ggctctgata
    15181 actgcggtga gtggaggctt tgggggaggc atcctctcac agaatttaga agcaagcgtt
    15241 tgactctttt gggggaacgg agtggcgctt gtgacgcagt cgaagagcaa taatagcgga
    15301 gctgctgatt gacgtcttga cggggcttgg gggagcctcc caggaaaatc agcagcccgc
    15361 aatttttttg gcctgatctt tagccgcgga cttccgcaga taaacgcaaa taagcgttaa
    15421 tccgcgaaaa tcggcggcta aagtttttta gtttcggtcc tgcagcagac gggaaggatt
    15481 cgtttcagca ctcaactcat ccagattgag tcgcagcatt ccgcccgcgt ctgaatacgc
    15541 ggtcgctgct ttctgctgtg agcgccggta agtgaataac aatccgccga tcaaaccgcc
    15601 cacgcccaga catagaagcg cagcataacc ggatgctttc aaaacagcga ccagcacacc
    15661 gagagttgtg ttgatgtagc gcttctcggc ttccctgaga atgtttggat tgtcgccgag
    15721 ccactgcacc acctgctcgt acttcacctg gtcaattaac tgtttggcgg tctgctcatc
    15781 tggtgcgtca aagacgaaca cggaatagtt tccgacgcga cgatacgcag tcggcgccgg
    15841 ctgccccagg cgccagagct cctgtatgcg agcgatgatg cgctggtggt tatctgaagc
    15901 aagctgcgga gtgtgaaatt cgatgattag aaccttgctg ggaccgtagg ttccgagcgc
    15961 ggcgtcagcg tcgccacccg actgaactgc cgaaaagact ggatgctgca agcccagctt
    16021 ttcgagactg gtaaaactgt ttagataaac agcgttcttt tgagcttcct ccggcggggg
    16081 aaggtgcttc gcgagcactc gcagatcgtc gtcgttcgag gtcgcagttt tgtcgttcgc
    16141 aattccacct tttggcgcgg gagtggattg agacctcgac acgtccgctg ccgtgaaaag
    16201 tagagacact aaaactaaga ttttcagaaa caagcgcaaa atgacgactc ctcagtgcgg
    16261 cccttcgaaa tctatgttta gaaaccgctc acggctttga tgttccgtca caccggttgg
    16321 gcagcacgtt ggtaagttta atgccagcgt ctgagttcgc gcaaaagaag gtcggcatag
    16381 tggcgcggct tgctgatgtt gtgcatcacg attgtgccgc caagctcgct ggcgttgtcg
    16441 atgatgatat cgccgaagcc catcagtcgc tgcggaattg tggctgagac ggttacgtcc
    16501 tggaccttgc tcagaggaat gtttttcgtt gtgcgggcga tgaggccggt gtcaatctgg
    16561 agcttcgaat cggtgacggt gtagcgaacc atgttgcggc ggatgtggta gtaagccgga
    16621 atcagcagca gcgcgagcgc gatcggaatc gagattgacg cgggaacgct cagcgacgcc
    16681 aaaagaaaca cgagcgcaat gcccgcgacg actgccagcg cataaccagc cttgacgaac
    16741 atcatcgtcg gtcgaacagt gaataccgtg tgctcgagat cctggtcgcc gctcatctcg
    16801 agcgagcgtg cttgcaagcg tgcgatgcgc gttgcttcgg ggtcagaagc tggtgcgccg
    16861 cagttcgaac agaaacggac gcttgggggt acatatgctc cgcaactgga acaggtcatg
    16921 gtgccaatca tgccacagat atacgcagat tgataacgat cggatcaatt cgtgtccgat
    16981 aagctttagc ttgtcggaag gtcgcgacaa gctaaagctt atcggacatt aatccgtgtt
    17041 ttctgtagtt actggctgac tagttgcaga cggccgtccg gcaggcgcgt cataaagacg
    17101 ctgcgctcgt agacgttgag tggggttgga ttgttttgcc cggcaggtaa actgctcgcc
    17161 tgggcgatag cttccgggtc acgaatcagg ctgtagcgat tgccgatgcg gcgcacgtac
    17221 tcgcgtgtct cgctatacgg cggaacctgg taaccatatt tcatcacggc gccttcgccg
    17281 gcgttgtagc ccgcgagggc gagtcggaca tcaccgttga aaagattgag caggaagcgc
    17341 atgtagcgcg cgccgccttc gatgttctgt ttcgggtccc agatattcgt gacgccaaag
    17401 cggcttgcag tggcaggtat gagctgcatc agaccgcgtg cgcccttgtt ggacatagcg
    17461 cgcggcttga agctcgattc ctggtgcatg atcgagtaga gcagcagtgg gtcgactgag
    17521 ttgcgctttc cggattcgac gatgtagttg tcgacgtcgg aattcccggt ggtgaagccg
    17581 ccgagcgacg ggttcaacgg catactggtg agctgtatca ggcgtttagt ggcgggtttt
    17641 gaaggagtct ggattttgac tgcgttgggg aagtcgaaat tatcgacctg atactgcgcc
    17701 gccgcgggtc cggctgccag agcaaagatg gcgagaatgt agagcgttct tcgcactctg
    17761 gcatcctacc acggtagtta aactcttgcc aacattgttg accacgacag ctcttgacag
    17821 gcgcggtatc atagcggcga tttttcggac cgccagccct cacggttaaa ccataaaccc
    17881 gcgcgcttct gcgcgcctca caaaaggaag aacaacatga agaaaatcct tacactttct
    17941 ctactactgc tctgtgtctt caccgttgga gtgcgagccc agaacacaac ctcaaccaca
    18001 gcgaaatcag cgacgaagca gggcgccaaa cgcggcccca tctttcgcgc tacgaaggaa
    18061 cagatcaacc aggctcaggc gattcttaag gagcggggtt tttacggagg agagcaactc
    18121 ggcaagcttg atgatgcgac gcgagaagga ctgcggaaat atcagcaagc ggaagggctc
    18181 aaggtgaccg ggacgctaaa taagatcacg cttgagaaga tgaacatcaa cctcactgag
    18241 aaacagaaaa ccatgtaaca cggatggacg cggattggtg acggattaaa tccggatcca
    18301 atccgcgtca tccgcggtta actaaccaaa cgtttcacga cctcaaagct tttctcgagt
    18361 gctaactgga ggttttcagg ctggtttcca ccaccctcag ccatgtcggc cttgccgccg
    18421 ccacggccgc cgatgatcgg tgcgagctct ttgattacct gtccggccgg aactttcccg
    18481 gtcagatcag ccgacgtccg cacaatcagc gaaacctttc catcactgct ccgcccaagc
    18541 acgaccacgc ctgacttgat ccgcgcgagg agcgtgtctg aaagttgtcg catcgccgcg
    18601 gcatcgagtc cgctcgcctc gcgcgccaga actttgacac cagccacttc acgcgactcg
    18661 tcaccattcg acgaagctcc ggctgcgcca ctcgcgatct tcaacttcaa ctcgtccgtt
    18721 tcgcgacgcg cctttctcaa ctcatcctgc aaacgcgtca ccgtcaacgg caaatcgttt
    18781 cgcgacgttc tcaacccgct gctcaatccg tccaccagta cttcagcttc acggaaccgt
    18841 tcgtaagcat ccatgcccgt gatcgcgcgg atgcgtcgca cgcccgaagc gatcgattca
    18901 tcactcgtga tcttgaacaa cccgatatcg ccggtcgcac gaacgtgcgt gccgccgcag
    18961 agctctttcg aaaaaatgcc ctcgacgcct ttgatcgaaa gcacgcgtac ctgctcgcca
    19021 tacttctcac caaacagtgc catcgcgccc gagtgcatcg cctcctcgat cgccatctcg
    19081 tccgtttgca caggctcgtt gcgcaggatg tgatagttga cgaggcgttc tatctcttct
    19141 ttctcagcgg ctgtgagcgg ctggtaatgc gtgaagtcga aacgtaaata agtcggtgca
    19201 acaacagatc cggcttgctt cacgtgcgtt cccaaaacct ctcgtagcgc ggcatgcatc
    19261 aggtgtgtcg cagtgtgatt gcgtctcgtc gcattgcgct tctctacttc gacgtcagcc
    19321 gagaccgtat caccaacttt aaaacttcct ttctcgactt tgatcttgtg cacgatcaag
    19381 tcctgcgcgg gtgagtaagt atcgagaaca gttgccaact cgttcgttgg accaacgatg
    19441 acgccaacat ctccgacctg tccgccactc tcggcataga acggcgtgtg gtcgagcacg
    19501 atctcgcctt cttcaccctc gctcagcgac tcaacctcgt cgcctttgat aacagcgacg
    19561 accttcgcgt cagtgacgtt cgtcgtttca taacccttga agatcgatcg cgacagccgg
    19621 ccagcgaggt ttgcgtaaac cggattagcc ttcgctttcg tctcgccctt gtcagacgtt
    19681 gtggtttgct gaagttcctg aagcgccgcg tcgaaggcac tgttgaactc gtcttcatca
    19741 atcgcaaacc cgcgctcttc caacccgacg cgaatcagat cgcgcggcgt gccgaacgtg
    19801 tcgtagagtc gcgcgagtgt tttgtactcc ggcatcgcgc ctttggttgt ggcaaacact
    19861 tcatcaagct tgttcaatcc aaccgtcaac gtcgatcgaa aacgatcttc ctccagccgt
    19921 accatcttct gcacaaactc gcgatgcgtc tcgagctcgg gatacacgtc tttcatgaag
    19981 tcgacaacga agtca
//
LOCUS       DASSFQ010000028         4697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_312174, whole genome shotgun sequence.
ACCESSION   DASSFQ010000028 DASSFQ010000000
VERSION     DASSFQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312174"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFH96_00900"
     CDS             complement(<1..416)
                     /locus_tag="VFH96_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972545.1"
                     /translation="MSKRSYELARRLELGAVALEAFASSLTEFEWQSRVPGDGRKIGV
                     VVHHVASVYPLEIELAQTVAKGEAVEGVTMDVINKMNADHAKKFDGVTKAETVELLKQ
                     NSVAAATAIRLLTDDELDQAAAVSLYANAILTCQFVL"
     gene            complement(428..1267)
                     /locus_tag="VFH96_00905"
     CDS             complement(428..1267)
                     /locus_tag="VFH96_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972546.1"
                     /translation="MKRNFFALIVVLGLLAAFVVVPRLADAQDDQAGASAVASGSDPV
                     AVFQAAGPTAASIQGSVDQFRAAVSVPAGRREINWDGGNIANEATSVVGNPFTGFLVS
                     RGALFTTPDGTGFVQAPAGTDVPPGGLAGFFNNPTYKTSFRAFSASRLFAVVGGKVTD
                     TTFFVPGGGNVAATVNGFAAVFSDVDQPNGSGPAKKRGNRNASTLIEYYGTDGQLLFS
                     SFVPASPGDGGFSFFGVVFPDARIARVRITSGDVAPGPNDSEKDDVVMMDDFIFGEPH
                     QVQ"
     gene            1546..4431
                     /locus_tag="VFH96_00910"
     CDS             1546..4431
                     /locus_tag="VFH96_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6972547.1"
                     /translation="MFAIYQTEEENGPNVSLTVNDKRIVFDRFSLDLVNECLWRGSEE
                     IKIRPKAFALLNYLLNRSGQLVTKDELLNAVWPETFVGDAVLKVTVRQLREALEDDPK
                     APRYIETSHRRGYRFIGEIAPEPKKEPQPQYQDTPDTAFSGAFFVYTGVVGRDQALAR
                     MHRWLYRMMRGDRQIGFITGEAGIGKTALVDAFARIIPADGSIRVGRGQCLEQYGTGE
                     AYLPVLEAIGRLCREDPRVAEIVRAHAPMWLLQMPSLLTPAEREALSRDVSGATRERM
                     LREMGDALEALTADVPLVLILEDLHWSDYSTLDLISYLATRRQRAHLMLIGTFRNVEL
                     IVSGHPLQVVKQELLAKQLCRELPLEYLSEAAVYDYLAVRFPGNRFPAGLAGLIHQRT
                     DGNPLFMVNAANYLLESRLIVQRDDRWVLDVDLANVELGVPDNIKQMIERHVDHLDPD
                     TQRTLEAASVAGVEFSTPALAAGLEEDPGVVEARCNELARQKQYIQECGVQELPNGEV
                     VTRFGFVHALYQNVLYDRIPAARRVRLHRLIAERGEEVYGNRAVEISAELAMHFERGR
                     DYKRAAYYFKTGANNAIRRFAYQEAVDLARHGIELIGDLPDTREVLNEKLCLHLTLGV
                     PLIAIEGYASPNVGEVYMKARQFYDQLGDSPDVSEVLWGLWTFHTLSGELETARDIAG
                     EFLRLSERLPYPGLAMRGHWALEITNTHLGNFELAVDHYEKALALYDPALHRDDSFLY
                     ALNPGVAMPCFAAWALWFLGRTDEALKRMDEAVALALELSEPHGLAHAKLFASILYQL
                     CGDYLRAQQYAEAVSEVSRSHGLVLYGAMADVMKGWTLSEQGREQDGIDLMRDGLAAI
                     EATATAVVRPHFLALLAEALSKLERVDEASALLEEATTIVDNVGERYYEAELYRLKGE
                     LLLKKDKRADAERCFERSLEIAKSQKAKSLLIRTEMSRLRVSSGTLL"
     gene            complement(4382..>4697)
                     /locus_tag="VFH96_00915"
     CDS             complement(4382..>4697)
                     /locus_tag="VFH96_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972548.1"
                     /translation="FLERKIGVDTPPPITHPEFFYGFVCVAVAWQVLFLILSRDPIRY
                     RPMMIPAMLEKIGFPIAVVVLYLQNRVAPTIFIPASLDLVLLFLFIAAYRKTRADDSS
                     RS"
BASE COUNT         1035 a         1285 c         1376 g         1001 t
ORIGIN      
        1 agcacgaact ggcacgtaag aatcgcgttc gcgtagagcg acactgccgc cgcctggtcg
       61 agctcgtcgt ctgttaacaa acggatcgcg gttgcggctg cgacactgtt ctgcttcagt
      121 agctcgaccg tttccgcttt cgtgacaccg tcaaacttct tcgcatgatc ggcgttcatt
      181 ttgttgatga cgtccatcgt cacaccctca accgcttcgc ctttcgccac cgtctgggcc
      241 aactcgatct cgagcggata aacgctcgcc acgtgatgga ccaccacacc gatctttcgt
      301 ccatctccgg gaacacggct ttgccactcg aattcagtca gcgagcttgc gaaagcttcg
      361 agcgccacag cgcccagctc gagtcgacgt gcaagttcgt aactacgttt actcatcttt
      421 tctcccctca ttgaacctga tgcggttcac cgaaaataaa atcgtccatc atcaccacgt
      481 cgtctttctc ggagtcgttt ggaccaggcg cgacatcgcc gctcgtgatt cgcactcgcg
      541 cgatgcgtgc gtccggaaac accacgccga agaaagaaaa cccgccatcg cccggcgacg
      601 ctggtacaaa actgctgaac aacaactgtc catcagtgcc gtagtactcg atcaacgtgc
      661 tggcgtttcg attaccgcgt ttcttcgcag gaccgcttcc gttcggctgg tccacatcgc
      721 tgaaaactgc ggcgaaaccg ttgaccgtgg ctgctacgtt gccgccgccg ggcacgaaga
      781 aggtcgtgtc tgtgactttg ccgccgacaa cggcgaacag acgcgacgcg ctgaaagctc
      841 gaaagctggt tttgtaagtc ggattgttga aaaagccggc gagtccgccg ggcggcacat
      901 cggtgccggc aggagcttgc acgaaccccg taccgtctgg agttgtgaac agcgcgccgc
      961 gcgaaaccag aaatcctgta aacggatttc ccacgactga cgtggcctcg ttggctatat
     1021 tgcctccatc ccagttgatc tcacgacgac cagcaggcac gctgaccgca gcgcgaaact
     1081 gatcgactga tccctggatc gaagctgccg tcggtccggc tgcctgaaaa actgcaaccg
     1141 gatccgaacc gctggcgact gccgaagctc ctgcctggtc gtcctgagcg tcagccagac
     1201 gcggcaccac cacaaatgcc gccaataagc ccaaaacgac tattaatgcg aaaaaatttc
     1261 tcttcatatg tatcccttct agtctttaag tcggacgaga ggatagagat cgatggcaag
     1321 ccagtcataa acttccggta aatgtgcggt aacgtttcgg gaatgaaagc gtggaaaaat
     1381 cggtaaggaa caaaagatga gaaaaaccag taatgtcctg gtttgaacca atatattctc
     1441 tgcgtaacct ttgcgacctc tgcgcctccg cggtgaatac gcctcttaaa ccgcagagac
     1501 gcagaggtcg cagaggctac gcagagcccg gctaactccc ccgccttgtt tgccatctac
     1561 caaacagaag aggagaatgg tccaaacgtg agcctgaccg tgaacgacaa gcgtatagtt
     1621 ttcgatcgct tttccctcga tctcgtgaat gagtgtctgt ggcggggctc agaggaaatc
     1681 aagatcaggc cgaaggcctt cgccctactc aactacctgc taaatcgctc cggacaactc
     1741 gttaccaagg atgagctcct gaacgccgtc tggccggaga cgtttgtcgg cgacgccgta
     1801 ctcaaagtca ccgtgcggca gttaagagaa gcgctcgaag acgatcccaa agcgccgcgt
     1861 tacattgaga cttcacaccg ccgcggttat cgcttcattg gagagatcgc gccagaacca
     1921 aagaaggaac cgcagccgca ataccaggac acaccggaca ccgcgttttc cggtgcattc
     1981 tttgtttata caggcgtggt cggacgcgac caggcgttag cgcgaatgca tcgttggctg
     2041 taccggatga tgcgcggcga ccgccaaatc ggtttcatca ccggggaagc aggcatcggc
     2101 aagaccgccc tcgtcgacgc gttcgcgcgc ataattcctg ctgacggatc gattcgtgtc
     2161 ggtcgcggac agtgtctcga acaatacgga actggtgaag cttatttgcc ggttctcgaa
     2221 gcgatcgggc ggctttgtcg cgaagaccca cgagttgcgg aaattgtgcg cgcgcatgca
     2281 ccgatgtggc ttttgcagat gccttcgtta ctgactccgg ctgaacgcga agcgttgagc
     2341 cgcgacgttt cgggtgcgac gcgcgaaaga atgctgcgcg agatgggcga cgcgctggaa
     2401 gcactcacgg ccgacgttcc gctcgtgctg atcctggaag acctgcactg gagcgattac
     2461 tcgacactcg atctcatctc gtatctcgcg acgcgtcgac aacgcgctca cctgatgttg
     2521 attgggacat ttcggaacgt tgagctgatc gtgagtggtc atccactgca agtagtgaag
     2581 caggagttgc tcgcgaagca gttgtgtaga gagttgccgc tcgaatacct gagtgaagcg
     2641 gcggtttacg attatctcgc ggtcaggttt cccggcaatc gttttcccgc gggtttggcc
     2701 ggattgatcc atcagcgtac ggatggcaat ccgttgttta tggtgaatgc ggcgaattac
     2761 ctgctcgagt cgagactgat cgtgcagcgt gacgacaggt gggtgttaga cgtcgacctg
     2821 gcgaatgtcg agctgggcgt accggacaat atcaagcaga tgatcgagcg gcatgtagac
     2881 catctcgatc cggacacgca gagaactttg gaagcggcga gtgtcgctgg agttgagttt
     2941 tcgacgcctg cgcttgcggc cggtcttgaa gaggatccgg gcgtggtcga agcacgctgc
     3001 aacgagctcg cgcgacagaa gcaatacatt caggaatgtg gcgtacagga attaccgaac
     3061 ggcgaagtcg tgacgcggtt tggctttgtc cacgctcttt accagaacgt tttgtatgac
     3121 cggatacccg cagctcgacg cgtgcggctg caccggctga tcgcggagcg cggcgaggag
     3181 gtttacggca accgcgctgt tgagatctcg gctgagcttg cgatgcactt cgagcgcggt
     3241 cgcgattaca agcgcgcggc ctactacttc aaaaccggag cgaacaatgc gatccggcgc
     3301 tttgcttacc aggaggcggt agatctcgca cggcatggta ttgaattgat cggcgatctg
     3361 cctgacacgc gcgaagtctt aaacgagaag ctgtgtttgc acctcacact cggcgttccg
     3421 ttgattgcga tcgagggtta tgcgtcgccg aacgtcggcg aagtctacat gaaggcgcgc
     3481 cagttttacg atcaactggg cgactcacct gatgtttctg aagtgctctg ggggctttgg
     3541 acattccata ccttgagcgg ggagttagaa acagcgcgcg acatcgccgg ggagtttctg
     3601 cgactttctg agcggctgcc gtatccggga ctcgcgatgc gcggtcattg ggcgctggag
     3661 attaccaata cgcatctcgg taatttcgaa ctcgcggtgg accattatga gaaagcgctc
     3721 gcgctttacg atcccgcgct acatcgcgac gactcgtttc tgtatgcact gaatcccggc
     3781 gtggcgatgc cttgcttcgc tgcctgggcg ctttggtttc tcgggcggac tgatgaagct
     3841 ctgaagcgca tggacgaagc cgtggcactc gcgctcgagt tgtctgagcc gcacggactc
     3901 gcacacgcaa agttgttcgc aagcattctt taccagttat gcggtgatta cctgagagcc
     3961 caacaatatg ccgaggccgt cagcgaggtt tcacggtcgc atggactcgt gttgtacggc
     4021 gcgatggctg atgtgatgaa aggttggacg ctcagcgagc agggacgcga gcaggacgga
     4081 atcgatctca tgcgcgacgg actcgccgca atcgaagcta ctgcgactgc ggtcgttcgt
     4141 ccgcactttt tggcgttact ggcggaggcg ctgtcgaagt tggagcgagt tgacgaagcg
     4201 tcggcgttgt tggaagaagc gacgacgatt gttgataacg ttggcgagcg atattacgaa
     4261 gctgagctct atcgcctcaa aggcgagttg ttgttgaaga aagataagag agcggatgcg
     4321 gagcggtgtt tcgagcgatc gctggagata gcgaagtcgc agaaggcgaa gtcgttactg
     4381 atcaggaccg agatgagtcg tctgcgcgtg tcttccggta cgctgctatg aatagaaaca
     4441 gcaggaccag atcaaggctg gcgggtatga aaatggtcgg cgccacgcga ttctgaagat
     4501 agagcacgac aaccgcgatc ggaaaaccga tcttttcgag cattgcggga atcatcatgg
     4561 gccggtacct gattggatct cgcgagagga tcagaaatag cacctgccag gcgacggcga
     4621 cacagacgaa gccgtaaaag aactccgggt gcgtgatcgg cggcggcgta tccaccccga
     4681 tctttcgttc cagaaaa
//
LOCUS       DASSFQ010000029        45500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1802971, whole genome shotgun sequence.
ACCESSION   DASSFQ010000029 DASSFQ010000000
VERSION     DASSFQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 45500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802971"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VFH96_00920"
     CDS             <1..1366
                     /locus_tag="VFH96_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972549.1"
                     /translation="ESVNLIDDRLPLKTLAYRLFAQDMFIRWIREMRTAIRSNGNTRQ
                     LITVGQDEAGLGDNPNMQFFANEIDFGSLHNWWANDDLVWDSVLAKTPSKLNLMEETG
                     VMFYEKADGGAAWRSEQDVSRLLERKLAISFAADGAGFIEWIWNTNPYMNSTNEVGIG
                     FHRVDGSAKPELEPFLRIAEFMRQYGQYLKDRVPEPVAMVIPHSQMFLPRSFAQEATR
                     RCVRAMYYHCGVPMQAVGEYQVPEYLDQAKLIIIPSPVVIRSKSWSAVNQETTRGATI
                     AISGAIDIDMLPPSSFSTLPLRNVPVAQSETISIGGREYLVRYEGEKMQRLEKAVDQS
                     KAPPGQLFNLSFGSGRVMWSPLPLELGDSTAALVAFYNLALSTAGVSPIFTATPRTPA
                     VLILPSLFRDVVLYTFVSETDRDTQLQVTHLESRTPFPVTIPAQRTAMVLLERKTGTI
                     IGRM"
     gene            1793..2734
                     /locus_tag="VFH96_00925"
     CDS             1793..2734
                     /locus_tag="VFH96_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET6972550.1"
                     /translation="MESLNLDLVAIIWKVRHSFSSYLDAEIEKNLSIAPNLKLITFLI
                     LVLLIDSVVLGCRQPAATDEKNSNPWQLVWADEFNYTGLPDPQKWGYDVGGHGWGNKE
                     LQFYTDRRPENARVENGHLVIEARREPWQGSQYTSARLVSKGKGDWTYGRIEVSAKLP
                     SGRGTWPAIWMLPTDWEYGKWPDSGEIDIMEHVGFDPDVIHASVHTKSYNHSINTQKT
                     AQLGVSNARTGFNIYAIEWTPQEIRAFINKEHYFTFKNERLTNPSADFAQWPFDKKFH
                     LLLNLAVGGKWGGAQGVDDAIWPQRMEVDYVRVYRAN"
     gene            2785..5940
                     /locus_tag="VFH96_00930"
     CDS             2785..5940
                     /locus_tag="VFH96_00930"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972551.1"
                     /translation="MSKTLSLFLLLFFCVTPFAAQTTADDYNATFKPLKWRSIGPFRG
                     GRSVCGTGVVGDPKTYYMGTTGGGLWKTEDMGLSWRNVSDGFFKTGSVGAIAVAESDP
                     NVVYVGMGEHAVRGVMTHHGDGVYKSTDAGKTWKKIGLDASQHISRIIVDPKNPNIVY
                     VAAQGALYSHTKERGVYKSVDGGATWKSVLYVDDKTGAAELSMDMNNPRILYAAMWEH
                     GRLPWKVISGGPGSGLYKSTDSGETWDKLKDGLPEEMGKMSIAVSRSNPEKVYALIES
                     DSDKEAGGLFVSSNGGKKWSRITNDHRLVQRAWYYIELFIDPKDENTIYVLSASALRS
                     KDAGKTWETLSGTHGDYHDLWINPDNPNNFIISNDGGSAVTFNGGKIFSTQSNMPTAQ
                     FYRINVDNQFPYRIYAGQQDNTTVSIASRELGGGGIGPSSWMPSAGGESAFLAFDPND
                     VKYVLGGSYQGTIEALDVKAQASTNIMAAPIQYLGMDAKDIKYRFNWNSPIVWSKYEP
                     NTYFHGAQYVLKTTDMGRTWKEISPDLTRNEKEKQGKGGEPYTNEAVGAENYGTLAYI
                     MESPYEKGVIWTGSDDGFVYVTRDGGANWKNVTPKGLQECLINAIEVSPHDKATAYIA
                     TTRYKFNDHTPGLYKTTDYGNTWTKITNGLPANAFTRVVREDDGRRDLLFAGTELGLF
                     ISWNGGRDWSPFQLNLPITPITDLRVHKGNLIAATSGRAFWVLDDLSVLRQYKKDAPS
                     FAIYRPDGAYLVNGSSELDRTDNEFTGASTFRGVNPATGVVLYYQLPELKKDDEISLE
                     IKDTSGATVRSYSSKADEKFVTYDSGPRPDPTLPKGKGLNRFMWDMRYETMVGVPGIR
                     IEGGFAAHKAPPGRYTVVLKMGGQTVTTDAEILANPLYPTDASTYTQYHQTMLAMETE
                     LTAMHRMVNSLYEKQKQLESLLSSLPSGEKYTAIKKDGEALLKKMKAWDEDMVQRKSK
                     AYDDVENFPNKFTANYLFLINQTESDIPRINQPSLDRMKELNAQWTSLKARGTELLNQ
                     DLPAFNKRLWDAGLGAIWKDS"
     gene            6242..7144
                     /locus_tag="VFH96_00935"
     CDS             6242..7144
                     /locus_tag="VFH96_00935"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET6972552.1"
                     /translation="MFGKFTAHHDAGLLTGLEATKDQELLSYLLIHRDRPHSREALAS
                     LLWGDTSTEKSKKYLRQALWHLHAALNCDNGNNSEVLMVDHDWLSLNPCSNLWTDVAD
                     FESAFLAAEGIPGRQLDNEKARGLKDAVTLYNDDLLPGYYQDWILFERERLQNMYLLM
                     LDKLLVYLQFRGEYEVAQGYGATILRYDPARERTHRQLMHLYSLAGDRTSALRQFDRC
                     AHALKQELGVKPERKTVELYERIKSDQMGHTDVPENSHSSTSPDILTRLKQLQAIVSG
                     VQRRIQRDIKSIETLHSNDKTSRG"
     gene            7246..7479
                     /locus_tag="VFH96_00940"
     CDS             7246..7479
                     /locus_tag="VFH96_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972553.1"
                     /translation="MDLTESKVHSFIVKLWLEGDANSFVWHGHITHVPSGERRYFKKL
                     SGITDFVSEYLDGRKAQPDLKSRMKDWLKKRRA"
     gene            7486..8067
                     /locus_tag="VFH96_00945"
     CDS             7486..8067
                     /locus_tag="VFH96_00945"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HET6972554.1"
                     /translation="MGTFQAIAATGQAMLGLLSDAVPRDQFPNAQFELYQMSNFQQPM
                     EEGISLFLYRIAANTSRRNLPPTTGPDGRRFRPPIPVDLYYIATAWAPTAVRQQRLLG
                     WTIRMFEDVPILPTGLLNNYGPEPEIFKNGETVEVVLDSLTLQDLNNFWGVSKSSLQL
                     SVGYVARMLHIQSSMPLTEYAEVQTREFGVGKV"
     gene            8071..9027
                     /locus_tag="VFH96_00950"
     CDS             8071..9027
                     /locus_tag="VFH96_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972555.1"
                     /translation="MAAPFITLDRLTMVAPLGLRFHDSTTGTIVDDGLVVWAYPVGRP
                     TAKRQAIANRKGVYVLQHGYGLRARENGDGSRFYWDHPVPPNKDFVIEVTDLQRRFQS
                     FQFTASLPAEGIFKWLDSPLSTRTTVPLFPAPTQSVASGMAVIRADLWDTSIDAPAAW
                     AVIEAFTSGTGGKFLGRGIADDAGRIALIFAFPSPLSFAPVSPPGSPLGSPPIATSPP
                     LTEQVWPLELHAFYTPVRPVVSPPDFIEEVGPPLPDLRFTLSQPAATLWADADQTEIL
                     ETNLRFGRELILKSRPSPSSPPSPLSQPRDSVLFISPAVSPP"
     gene            9037..10899
                     /locus_tag="VFH96_00955"
     CDS             9037..10899
                     /locus_tag="VFH96_00955"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HET6972556.1"
                     /translation="MPEYLAPGVYVEETSFRSKSIEGVSTTTTGFIGPARYGPLDLEP
                     DIITSLGEFERTYGDRQQLNFSDAVGMDNYLWHAARAFFEEGGKRLYVSRVFRPAADD
                     DDGRASATLPTSGGAGAVTISARFPGEAGNMRVRFDLLVGQNVLGTDENGDARVAGLF
                     DGDVVWMADTASPIGSPLGTGQLFRASFLQETQAWVFRSSGSPGSELGLDQLNADGGD
                     VIRTITVSVTVIPNDPSTFPETWAALPVEPGHLQAGMPDSLSAKFAAHPNNLAQARSL
                     PIVIELGANVDTGIEVLTALFGLNATDFSNPDQPLFVEVSLTGGNDGARPTAGEYEGE
                     EEPGKTGLKIFEDIEDISIVAAPGSTFGYENGYRADANTITNLLITHASRMRYRIAVL
                     DSGNAQSISQVRAQRARFDSTYAALYYPWVRVLDPVTRKEINLPPSGFVAGIYARNDI
                     NRAVYKAPANEVVNLALGFEKLLNKSQQDVLNPEGINCFRFFEGRGFRLWGARTISSD
                     PEWKYVNLRRYFAYLEHSIDKGTQWAVFEPNGEALWGNVRRTIEDFMLNEWQMGALLG
                     DKPEKAFFVKCDRSTMTQNDLDNGRLVCLIGLAPLRPAEFVIFRIGQWTADRKV"
     gene            11027..11479
                     /locus_tag="VFH96_00960"
     CDS             11027..11479
                     /locus_tag="VFH96_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET6972557.1"
                     /translation="MAVLRDRPYVQFNFLVDLGTGNTDGPEAGFQEISNIGMEVTVSE
                     YRNGNSKENSVRKITGLNKATDVTMKRGVIGSLNLYQWLNDIRNGNQNAERTVTIQLQ
                     NEDHTAVVQTWKLLRAKIIKHVSGPVNAKGTDVAMEELTLAYERLEME"
     gene            11484..14417
                     /locus_tag="VFH96_00965"
     CDS             11484..14417
                     /locus_tag="VFH96_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972558.1"
                     /translation="MSARAPRRLPGFRFETQAPPLPEVLPRMDIAVFVGFAASGPLQT
                     PVVVESEAQFTAIFGDDTPLAWDLVRGEEIHAHLGPAVRAFFRNGGQRCWIIRVARQT
                     ASAPQPLNRARYNYFPLPYLARAAFNPNGQIASLTPAFARGRSEGSWSDALRVSTALL
                     SRPIQVNGALLQDGVGYALSIVRDPSNPLAAGDMIRLTYESAGVSVLLALQTITSVLS
                     SPLGAPLVNATVSRAVWLLSMSSDPSAPSPNTPVTAAVFTREQLSSPPNPEDDIAAFE
                     ITYNAVFVDKKITLKLLDCPLADAPPPGSIVRIDQGGNHWWMTVDSLDFTSGDEGVPV
                     LSGSASRIIDPPNPLPVATPAGERLSFEIWVRQAEEYSISLSDLGFAPDHERFWAPLP
                     TDEEVYRLTDTPTTENPATLLWKQVGDLFRFPLAGLGPADEIYFPLLMPSLAENYLGP
                     VALPGSERERDGLAEFDAALFLDPDLVDIGAANLATTADFLQYLSPRPRRLTGMHAAF
                     PLEEATIIAVPDAVHGGWVKHEREPLIDPQPSPPPLRPEWWHFLDCNPAPKKTPALGS
                     CDPEPPEPSPIKPVHEPEWGNFLSCSIQIIEPPQLFAFPQFSSDGNLSLRWELSPPQE
                     AHYVLEESSQPNFSDAVTVFEDTTSSFTLYGRRTGDYYYRVRAIIGADTSDWSNGVAV
                     RVEDEESRWIVDTKDYSPDVLLAVQRSLLRFCAARGDMFGVLSLPEHYREDKAIEHTS
                     LLRATPNVVPPTEGVSALSFGEVNVFSYGAVYHPWLIGRENQNDTVVSMPPCGAVSGS
                     IAESALTRGAWIAPANRPLRAIVALKPTLLPERRLELQDALVNIVRQEPRGFLVLDSD
                     TLSADEDLQEIGVRRLLILLRRQALQLGVTYVFEPNSPAFRRAVDRGFTEMLDGMFER
                     GAFAGANAATSYQVVTDSSLNTPQSVDLGRFIIELRVAPSLPLRFLTIRLVQSSDRTQ
                     ALEVR"
     gene            14421..14891
                     /locus_tag="VFH96_00970"
     CDS             14421..14891
                     /locus_tag="VFH96_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET6972559.1"
                     /translation="MANPEKEQVAYPFTAFNFAVEIKVDGVAMQVVDAAFSECDGLEM
                     TMDVKTIREGGNNGRQIRLTGPLTFGQVTMKRGMTSNLELWDWFNLMLSDQSLRADAE
                     VVIFASDGETERARFQLSRCVPVKLKSPALNAKDGAVAIEELQIAYEKLTLKKP"
     gene            14902..15648
                     /locus_tag="VFH96_00975"
     CDS             14902..15648
                     /locus_tag="VFH96_00975"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972560.1"
                     /translation="MPGSQKLEKAELRELDAEFKNEINTDKKCQVQFNPETLKVSFAN
                     QVATPSGAGDQKGTPARQFVGAGTTKLSLQLWFDVTAPMPPEQKKEQDVRKLTAKVAY
                     FITPKPEGDKFVPPAVRFIWGSFQFDGIMESMEESLEFFSSDGRPLRASVTINLTQQK
                     ITEFTFRATAGPGAKPSPGTRPLTPAPQGKSVQSMADSQGKGDNWQDIASANGIENPR
                     LLQPGQLLDLNASASVSVGGSFQAGGSISI"
     gene            15664..15828
                     /locus_tag="VFH96_00980"
     CDS             15664..15828
                     /locus_tag="VFH96_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972561.1"
                     /translation="MAVVVNEVEVVPAERAAPAEQEKGGGGEQAQPSEHEIRRMVEQQ
                     MSRCERVWAY"
     gene            15835..16899
                     /locus_tag="VFH96_00985"
     CDS             15835..16899
                     /locus_tag="VFH96_00985"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HET6972562.1"
                     /translation="MADETTGLKESRPTVVVNNEVNGPLGGGLLTLMIVENTNGLYRC
                     EAKFGNWGEKNSTTDFLYFDRSVLEFGKDFEIKLGNDSIFKGKIMGLEGNFPEGQSPE
                     ISVLAEDRFQDLRMTRRTRTFTDVTDADVIKQIATDHGLTPEVDLSGPTYKVLAQVNQ
                     SDLAFIRDRARSIDAEVWMSDKTLHAKAHTGRNGDKLQMTYGNQLREFNVLADLAMQR
                     TSVAVNGWNISNKAAMQFEATDSAISSELNGDTSGVSILQSALGERKEALAHTIPLND
                     QEAQHEAEAFFRMSARRFVVGHGIAETNKSLRAGSQLDLQGLGPLFNGKYYVAEVKHI
                     FDSTKGLRSEFRSERVGIGK"
     gene            16903..17616
                     /locus_tag="VFH96_00990"
     CDS             16903..17616
                     /locus_tag="VFH96_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HET6972563.1"
                     /translation="MEKPPLEMILDVRLPSGLGGRWYGAFPALVTDIVDPEGLGRVKI
                     ALPWSPDTAGARYETWARLATFMAGNNRGSWFIPDVDDEVLIVFEGGDPRRPYVIGSL
                     WNGKDKPPESMDGAGKNFKKVLRSRNGVKLTMDDTDGREQFILETPGGQKLSLKDGPG
                     AVEIVDSNGNSIKLETSGITITASAKVTINASQVAVSAGMVTVDAAMSKFSGVVQCDT
                     MISNSVISASYTPGAGNIW"
     gene            17788..19827
                     /locus_tag="VFH96_00995"
     CDS             17788..19827
                     /locus_tag="VFH96_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972564.1"
                     /translation="MAKDQVQWAAPSPLWPIAAGASDIALRRNALRKPVILRFAADTF
                     MDDFVNLLETDPSRMHELVATPETWRGPTAEPAPAKPAPLFARELSRLGLAAAKKKGL
                     VPSPSGKGVSSLLTLNTAAQPKKLKLYQPAHQRYYLVASSLVCGRAGLPDRAINAGRQ
                     EKVGYVVRRMFPPGPLDINTPLPAFDPATWEEYAYVATATGNRWQRIPKQSDGLLIDG
                     EDQLPLFAMNFTEDDGRGRRVVAGLVPVGKREAYMGAGMKQQAGDPAPVVEPQPISDP
                     RMILFWSQVTEPWKKLLEQAANGSKMNKGSKIPDDPNAAPPPTLNLNLDNTASTAQTL
                     REQIQTGSWYVLLDFANLLIEQTPRVWRLLNGQGPPAGEPAFNAAENALATAITGTTF
                     SLTDSQKDVFRANTAYSRPAIKSSLKDALLAVKTGSIEANLEAVKTSFDRKRASLDPL
                     WPDFLFPLADPLLPSPMTTTALLDQAKDSVDALAVKIQAALPAAPVAEVPNNPLVSQQ
                     PMDMREGWFVIRCVFTRPECGPIDPPLLSEPTAPFQMAGFFDPDAPARPIRIALPLDT
                     SPAGLRKFDKNTAFMISDMLCGQIERVKGLSLADLVLSVLPWPLHKDLSVPDGGPCKS
                     DSGIEFGMICSLSIPIITICALLLLMIIVFLLDLIFRWVPFFIFCFPIPKLKAKA"
     gene            19986..20312
                     /locus_tag="VFH96_01000"
     CDS             19986..20312
                     /locus_tag="VFH96_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HET6972565.1"
                     /translation="MSWSEGEANVRESIRVILMTEQPERLRTPKFGGSLGRFLFEPNT
                     VSTRRSIQDRITKELAQWEPRIRVESVNVDPDPSDPQAAIATIIYKLVATQAVERVSV
                     GVSLGA"
     gene            20315..22639
                     /locus_tag="VFH96_01005"
     CDS             20315..22639
                     /locus_tag="VFH96_01005"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HET6972566.1"
                     /translation="MPLQIPSIDDRRYQDLLNEALARIPVHNPEWTNFNKSDPGVTLL
                     ELFAFLTENLLYRSNQIPERNRRKFLQLLGVPLQPASSAQGLVSFNNDRGLPETITLN
                     SGLEVRAGQVPFRTDHGLDVLPIEAAVYYKQRIVSPDTAVTDYYKLLYASYNGQPPND
                     TEIQLYQSVPIDGREGNEVAIGQDATDRSVWVALMVRSTDKPVDDSEGAKEDLRDKVR
                     KAIAGKTISLGIVPAIEAASRVLAPGGSANPEGEPLLQYLIPNVRSDGLLPTDPAQRV
                     PQYRPLVASPLGNVLEMPGVVEITLPSAGELKLWSNLDPLEQGVADFPPSLEDSRLSD
                     RVITWLRIQASPGVGARLLWIGINTVTVTQRAHITNELLTDGTGAPDQVVVLSRKPVI
                     PNSVLLTVSPPASDTQTWQEVDDLLAAGPEVPVPDLRLPPGATPVPPLPSEVYVVDAE
                     SGQIKFGDGLRGKRPPAGAKLRASYDYGVGSAGNVAATSINSSPALPAGIKVTNPVRT
                     WGGTEAEQVSDGEKQIARYLQHRDRLVSADDFETITMRTPGVDIGRVEVLPAFNPELV
                     PNEAGDAPGAVTLLIIPRNDPNQPDAPVADAIFLDTICRYLDPRRLVTTELYLRGAVY
                     KPVWISVGINVVAGVATAEVREAVKRALLDFLAPISSVSGLTDSLTSLTTPAYASAQN
                     GWPLSKAVTARELLAVASRVSGVLLVNDVLIAEDTKPSSDQIPMNGLELPRVMGISVS
                     IGDPMDVDSLRGQAPAAAAPATRIVPVPVIPEEC"
     gene            22647..25439
                     /locus_tag="VFH96_01010"
     CDS             22647..25439
                     /locus_tag="VFH96_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET6972567.1"
                     /translation="MDANGTRFHLLLGRDDWGQCTVDTASGKKSLKELWGLLGSEPGL
                     FWNEERNELTLEPRLFKFTAAPKDTFPLLENRRGAGRDRYGNWFWIDETSLKIRVRSI
                     GSGLVTDFWPLEQKCLAPKSKGDFTPLNDAAPATLLKLSGLAITEDHYLVIGVTEPAG
                     LLIFDMHTGQEPRQILWPSSVDFEPFDMAPRPGGGVYILDRFNKSYWALDRHFNVIGP
                     EENGETILAEPREDDFQPVDESEVRGTKRRVFPRNFSLLQSPLALNDPIAIEALPDGT
                     VLILDFDPGHRFSRIFRYGFNQQLSDQLTTEAILSLIEEDAQSEFTLVGYDFAVVTNR
                     LYVVAADGNQSYVFRMCLRDEKVELQPIAEYLPMRLFGGKALAGTSDGAYYDFAEDWI
                     PLVQQRRPRYVPEATLETPLKTALDKPYFDSGETDCVWHRIMLDAHIPPDTTIEIWSR
                     AANEEIDLPLLPWQREPAPYLRGDGSEIPFLRATVSNACVGVANKKIGAGTWELLFQR
                     ARGRFLQLRIRLKGDERSTPRLRALRAYYPRFSYLSNYLPGVYREDTQSASFLDRFLS
                     NVEGLYTSLEDKIAAAQILFDVRSAPSEVLDWLAGWYGVALDPSWDEARRRMFIAHAM
                     DFFQYRGTIRGLTMALHLALDQCADETIFEATRPLEPIRIVERYRTRTLPGVIFGDPT
                     ESTGPRAVNTGTSWRPDQGRANLNQRYLDALGSTANPLTQYPLVPPTDAQENATWTQF
                     SLATLGFVPSAAVPGPGKLQLWQDFLARRYRRIKSLNTVYQTTYTGFESVPLPDQLPF
                     DGAPLADWFQFESVVLRMQSAAHRFTVLLPVPLSLAFNPEEHQRRMDLSRRIVELEKP
                     AHTVFDVKFYWAMFRIGEARLALDTLLAQGSRAPQLLPELVLDQGFVGESYLGAPVPE
                     NACDRFIMGRDPLQNS"
     gene            25455..26834
                     /locus_tag="VFH96_01015"
     CDS             25455..26834
                     /locus_tag="VFH96_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972568.1"
                     /translation="MSCSCFSSNTNSMTLAEPNPAKHVNFNVGMVLGVDDFTQEFAYL
                     SGRDQWLARDLIGYGTVRGLNVRIEVDAIKGPRVVVEAGVALNPRGQLICVPAAQCAY
                     LKDWIADHSADITPQVSSPPDSDLQLYVVLCYRNCPTDDVPIAGEPCRSEDKLMAPSR
                     LSDDFVLELRLERPNQREEDAVRDFMAWLKQVHISQVDPSTPLDQFLQAIRDAAAVWL
                     ASPLSSPPGDFMFGSPPGSLVINAADVSEYLRAAFRVWVTELRPRWIERWHGCAATHI
                     EGDAAGDEDCVLLAQLDVPLLPISPGTFDIPNAPIPVNQNDRPFIVHLRMLQEWMFSN
                     LAMTISAFTGTGGGGGLDFDVVSLKPPQGAPISNVDGPISFELKDEQIVIANSTNGVV
                     RMVLPPTAGQDGRLMVIKRISTGSQVQIGANAGDLIEGQASLILTAQNRFVQLVANEK
                     LKTWHVIAQ"
     gene            26882..28135
                     /locus_tag="VFH96_01020"
     CDS             26882..28135
                     /locus_tag="VFH96_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972569.1"
                     /translation="MDSQISLQQPILDGGIRSINFFNGRLLSARDLTREQSANREADR
                     RLGQAVGEGIAYGLEVSKSTASTQESPVITVEAGLAINRLGQTLMLSAKTEVALVRKA
                     TPGPDNTDPFSECVPLQSGTYIAGAGVYLLTIAPAQGTEGRASTHSLESTNASCNTDT
                     NVNAVQFRLIQLDQQISAQEMLDQTKLRNLIAYKCFGVDSTSKFPVNPFTTNLEKYGL
                     LDDLRPTWLTECEVPLAILYWTLVDGIKFIDLWSVRRRLARRLTDKTWSFLTSERTTR
                     EGEAMYLQFQSQLKDLQTGGVIASQVKARDHFKYLPAAGLLPVGGNGGFDYTTFFNEI
                     TIRQPIFIEGAQLRSLFQESFEYPPVETSSELVFYLYIVRENAQSSLGGALSTQSYVV
                     FASGHTLYRGEARYDVHHWNFGNFS"
     gene            28148..29383
                     /locus_tag="VFH96_01025"
     CDS             28148..29383
                     /locus_tag="VFH96_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972570.1"
                     /translation="MAQLLNHAQPGDVITADDWNLVVDAINELLQAGQTSGIKIDNVV
                     PAGSALDPFKIGSLVQVVGQNFGFSIGQSKVIFEAGGTSVTVLRAQMLGGSFDERLMF
                     MMPPIPGITPAGLAMTMKVDNGVASDNRSVFVMPVVISLIGDIFVGFRADVNPNPSPN
                     PVVSTQQADFAFRLQSGTNIPASYDLSADIINSSVAVPASLVSSIQFRENNATITSKK
                     IDMGKSEIRDITVRIPQIPVTFIAGTSFTLKLTATSGSVTGTDQRSITVGTALPVPDP
                     KIEVIQLQPQIFNVQTGNNEVDPANGRLEGSTIKLKTGRQMVIGFNVKLTQNGVYQVT
                     IQPRQGTTLTGWGPALVDTQTTITVPTNNDQTTRLLQFGVTTSTVQTPSPTGTVIFKI
                     KRDTATVEFNKEFGVELLP"
     gene            29444..31324
                     /locus_tag="VFH96_01030"
     CDS             29444..31324
                     /locus_tag="VFH96_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972571.1"
                     /translation="MTVSIPSAGGVPGASGPPDWLSGGAGQNFRFDDPRWRGATKLDF
                     AAGAAHGIFFRAIHDNSGQEFIYITLRAGFVPEMNTGRDFVYIGLQRHGTTDAIVIKI
                     QVHDSAFADAGPPSGSPPVNPFVIQVNQSSAGGPFSGNIVGNPNWINANARVWLQNNN
                     TVPSDPNFRWGVQIRIPVSGAGGILNTNGPQLGSDFDMWYLIRGSVMGMATILGEYRT
                     DGNNTSAFDLGNSNYPLPTGIWDEFLLTSGPASFGGVTIFGNGQNDVVVQNGLGTGTT
                     IQNGALNTFIARPRNYRAPGNDIASGDIAATFRIANWGSVATAAPWDFVDPAVTTPVA
                     STAVIPTLAAASNPPATSPIAQPVVMNLPAGKSTHQCVLCTLSSTGPVTLFLQDSVFR
                     NMNYDHASILEREAEIDTTGLPKDDAPDPRDVYIAIEKVGMEQNTPGADEGTFLSNRI
                     VRAMRRGGPIAEKLKRAQGLLDQIGRGAEGQNQRLEVLVNVLTEVGLTDDELDQVFPT
                     FRVHVYHDTGERLKEDGEEKPVLRAQSSFGLYMYHEGPVEGWQTSIQGATRISDNVYQ
                     MPVPKDGSAKIKVIVQAVDKGQDRIPEDPIMAPRGAGEIGTGPGDRKGCWLALWKLFG
                     FK"
     gene            31335..32951
                     /locus_tag="VFH96_01035"
     CDS             31335..32951
                     /locus_tag="VFH96_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972572.1"
                     /translation="MLGTFNDSTILRLRVKGSDLDPLALRGRLENVLSDVSLQPRLPP
                     SATLFIRKLDDPLPGSIQLDSPHARAPQAWRQAFQTRFDRLVSSAARPAHGYVADSAE
                     SVVFYDYAELLACLTADWCNGSALTRWWWQAFIKRGDLSWLIRKFWREQIEYVPAALE
                     HVVRKGVLVEFVSRLSDVEVREIVQRVVSTFGVTRILRIENADFFPVDEARQIEHVAQ
                     IRSEMPWSDVVPESDTPSLRPVQQFFVGIVLMVHRAPSRVRTLNFAREVEHWIQEHFV
                     EVQHAETILTEEIPEPELFTPAEPPTPIRVNPIHADPIAFKPPPPPAGSVKSVFRKAE
                     KITIANATFSYEEPNPVVIEQTPVSVIQSETTLPPEPIEVTTTTPESLDPATIETNLG
                     GLFYLINLAIFLEIYNDFTSPVEQLHDDLSIWDFVTIVGKELDPETDDPIWQLLSNLA
                     DREDELITPTWLTDLLPHIRARLHLALGIDDDLAEILLHHEAKITVSPTHLDVFFSLS
                     DHPIEIRLSGLDRNPGWVPAAGRFIAFHYD"
     gene            32956..34974
                     /locus_tag="VFH96_01040"
     CDS             32956..34974
                     /locus_tag="VFH96_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6972573.1"
                     /translation="MRKNLFDGLQPTPAEHFKLYFYGAVLSLFHHVVQVYGSSESAFK
                     EFPFLIGYNNELADGGLSGVHSTEAVSWWLESLQAWEEKATGHLPLRALRKSAQLDHS
                     ALILLTTVGLIEEDLRFGLVFETMQGSPGQHRATPSLLNTWGRDEAHWRDARTNLRQM
                     RELGLVRVVNPESPRLEWALEVPSALWDALRGEQNEIPGSGMRYRSPAELARMEDLIL
                     PPDVHEKVRAIPGLFRAGEAQTLIVRGAYRNGRRTIFSAIARELNLGLLEIKGIIGPH
                     DERFRVAGPLATALNALPVIVMDLEPRESIEIPRLSACDSPGGIVIGKQGGVSGEGAE
                     RALVITLDMPDPGMRKQHWQRACHESKLRDPETVSERFRITSGNIHRVARLAESYAAL
                     AKRTEITLVDAQRATRSLNRQILDALAIRLSASGDWSQLAVGGETRRELESLVSRCRH
                     RERLQSQADAGVRALFSGPSGTGKTLAARLLASTLQMDVYRLDLSSVVNKYIGETEKN
                     LNLILSRAEELDVILLLDEGDALLTQRTGVQSSNDRYANLETNFLLQRLESFEGILVI
                     TTNASERIDNAFQRRMDVVISFSPPSPPERWAIWQLHLPSDHAVDYSLLEEVAGRCVL
                     SGGQIKNAALHASLLALEDGGIVTSAMIGDAIEREYRKAGGVCPLRRN"
     gene            35173..39000
                     /locus_tag="VFH96_01045"
     CDS             35173..39000
                     /locus_tag="VFH96_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972574.1"
                     /translation="MLGKGLFRTHDGKIIELPKDITAEEAARLEAEALAAQKKIGKGP
                     PPQPVPDVKKLDKKEDKKTQLKALPKKKPGKGKGKRARGGGAAAMMKAAGTSKVAQYL
                     ASKAAPVVNKGFAKLDQLRTNQQTHDSADEKRAQSEQAQVIPDSEGQSKSNTGQVNVV
                     SDRPAPKVDPNKGKQTLQENLRENVPKSIEDVDNFKRDKKAQHTGADVLKVVQTDKSA
                     VVSTFQDMGTTPPPAPREFEPEPLPPPEAAPGTAALTLGKDGIAPLQKEHTDLSNFTK
                     EADGKLKEEGVTQEQLDMVDSGDLATANKEKKGMEKSAITEPLAVQQFAQQQNKKVEN
                     DLKQEESKKRSDMNAKRKGNLGVTGQKQKNTKSSLEKKREEVATKINGIYTAAQDKVK
                     KKLADLETAAMKRFDDGNNKATKDFEDTVNRELDAFKADRYSGWFGWARKAKDWLLGM
                     DDLPGVKAIFDRNREKFVATINKLVEDITADNKKVIQECKDELTKARTEIKDYVDKLG
                     PSLKDIGKKAAEEMNSKLDEMDKFIAKKEEDLQNQLKDKQTAAIKAIDEKIEKMKEAM
                     AGALAKLGKLLLWAAKKFFTWALEKFGFSLGDIENIINKGAAVLKAIFTKPIQFVKNL
                     MNAAITGFKNFGKNFLKHLQDAVFDWLTGSIEGFQLPTTWDFKGIISVALQLIGISYA
                     NIRRHMVSVMTEPVVVGLEKSFTLVKTLITEGPLAAWEQLKEMATDIKDAFIQAVKEF
                     IAQKIIEQAIQWIVGIFVPGAGIIKAIIGIYDTVVFFIKKAKDIIKMVGNFLGSVSEI
                     AAGNIGAAAQAMEDGLARGLLLVVNFLAALLRLGGVTAKIRAVLQKIRAKVDDVLLKV
                     AKWIATKAKAIINKVVGAVKGAVGSVTQWWKRRKKFKADDGQTHSLAFHGEGRAARMW
                     VESDPTPFETFIKDVKADVSPQKEAKARALITAKEMDELRNKASVTDGTLSDPDTVKL
                     QALWDKLADDVAILFAGGAPKEPKYGVPTSPAGWGSGMLILNLKNPPKGSRPTVSNPH
                     FIDLNIRRYGNVTSPSTESYYILGHLLNENLKGPGNIWENITPLSRKGNAQHVTQVET
                     DVKAAATSKIVRYEVQARYGRAASALLAQIPANTTDAALLNKRKVLLAEQYVPSTLLC
                     RAYTVDASGKPVGGFNLTKEVLNKVDDSSLDDYNVPGQAVQRLKKLAINDAIKKAHAG
                     EKKYEAALMQLPGIGPTRIVELINGGKPWGKWEDFWKGVNGVTKENTDDWRTNGIAGG
                     SVDVILNGTCEWV"
     gene            38997..42158
                     /locus_tag="VFH96_01050"
     CDS             38997..42158
                     /locus_tag="VFH96_01050"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HET6972575.1"
                     /translation="MTTATTKAHGPVQAQNVPSRVAAPKTKNVSRSGVTAQRKTHATL
                     RAFAPLRENPSTHVSLTISKKQPKNALIQTVQPPADVAPPTMTLGRPASQVVKDQTLK
                     KLESTTITSTGAPLPVAVKSPLTHSFSVDLTPIRVHTDARAQLTVRSFNTRAFAYGHH
                     IFLGPGESPSDLRLMAHEVAHVVQQSRGAVIQNFTTGKGDALEQEAERASGAAVRGEK
                     FKVQQRTSPRPQGLLGIDLGIPNPLDWLAGKANMIPGFRMLTIILGVNPINMSPVERS
                     AANILRALIEIMPGGGLITQALDNNGVFEKAGAFVEEQINALGLTGSMIKSAVTEFIS
                     SLSLPGDLLNPGAAWARAERIFTAPIDQIKSTAVNLVTGIIEIVKDAILKPIAKLAEG
                     TEGYNLLKGVLGKDPITGDPVDRSAENLLGPLLKMIGLGDVWQKMQESKAIPRAWAWF
                     QTTLSQLIGFVSEIPTLFLSAFKSLTLEDIILVPKAFAKLAAVFGGFMSKFVSWGVDA
                     MFKLLEIVFDVVSPGALGYVKQTASAMKSIFQNPLPFVGNLVKAAKLGFMNFGGNFLT
                     HLKKGLIDWLTGSLQGVYIPKALTLPEFGKLALSVLGITWAQIRGKIVKALGPNGEII
                     MQGLEAAFDIIKALVTGGPAAAWEVIKDKLTGLKDTIVSGIISFVVEAVVTKAIPKLI
                     AMFIPGAGFISAIISIYDTVMVFVNKISKIIQVVTAFINSIVAIASGNIGAAAAKVES
                     ILGGLLSLAISFLAGFVGLGKVTDKIMGVVNKVRGIVDKALDTAINFIIGKAKALFAS
                     LFGKKDKTPDSRTDAQKMADMKAAVSEGLTIVSDKSSSKKAKDKNLKKLKSKYKLTNI
                     ELMSDSKAKETETVHIHATINPTYDSNGIIIDAFPPAPVVKIVAIVGAPIPRQGMEEV
                     IEERAGYERAHLLGPILGHDLPQGVMYAPVEVNQKLQKSGIEGLIKAMYRQRFPGAEF
                     RVTLTATPHAGGGNMLASANYVLHGKLPSDSDWTFIFDYTIKVGLGASPSIVAKAGEP
                     PDMEAVNRFSTGVASLLARRERR"
     gene            42164..44191
                     /locus_tag="VFH96_01055"
     CDS             42164..44191
                     /locus_tag="VFH96_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HET6972576.1"
                     /translation="MYLDSVKLLQMQVGYGDLGMYGSLGYEGRQVTVRKQHYQHAFST
                     HPPARLHFHVDRRFASFACQVALNDDVAAGLSHADFSVVADGREVANEPYVQAGEPPR
                     PLVADISGAEYLELVVRTSRWENSHAVWLDPQVDESPAPVTHLFDSLSRAEMIIPERL
                     PRTERCIATVVSPGFEHLLDDMLGSLYANGCCNDALIVVFVLNGNNSVERVVAKYKAT
                     AVRCQSRAGLNPMSKAVMYSTARVVDAKHYLCLDADMVVLGDLRPIFATLEALPEGRI
                     LCCREGNSHNANHLGSAIGWIYGGGQADLSKLGVTPEEQAYSLVVNDGLFAGDQTALR
                     ALDGAIRAMPQACAWVDGHSKVKWRNQFIFNLALARLQCGVELDPLYNVQLHVQDVNF
                     FEQGSRLRATWRGREPRVLHFSGGAKRKYPQWQGKFSIADPLAGKTSGDSYAVFVEAL
                     RAWLGRHGVNALAWSFYGLTSGDSASVKDPSTLPLLATLHYLIRANGCVRVLETGTAR
                     GISAACLASAVAHREGGRVVTLDPFLHAGREELWAMLPEGYQSCIEARPVDSIAGMQM
                     ALDADEVYEAVLLDSLHEAEHVWAEFQVATEIVCPGGLILIHDATYAGGTVPATLARI
                     EAAGYNVVRLWAADGGTAEDDHLGLAVIENRRRRVPVILESQNEFAERVSA"
     gene            44163..44843
                     /locus_tag="VFH96_01060"
     CDS             44163..44843
                     /locus_tag="VFH96_01060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HET6972577.1"
                     /translation="MNSPNGFPPDFESSDIEIIRAVEPYTMTSTERIHALIHAVRHIV
                     RNRIAGDMVECGVWKGGSVMAMALTLLQLGERDRSLYLFDTFSGMTAPSDVDVDYQGQ
                     QAHVILDAVRCEASQQEVEKAVLSTGYDRERIHFVPGRVEETIPLSAPASIALLRLDT
                     DWYESTQHELLHLFPRLARGGVIIIDDYGHWRGARQAVDEYIAENQIPLLLHRIDYTG
                     RIAVKISV"
     gene            44870..>45500
                     /locus_tag="VFH96_01065"
     CDS             44870..>45500
                     /locus_tag="VFH96_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HET6972578.1"
                     /translation="MGYLEDSLNLTLGQIIPLMQQRIMNESTYFGVKTWKCPIDFWIY
                     QEMIFELKPDVIVEIGNHSGGSTLALAHICDLLDNGRVIGLDLSHAAVPHKVKEHQRI
                     KLIEGDACANFDRVAQLIKPEDHVLIIEDSSHTFENTLAVLDAFSRLIKPGGYFIVED
                     GICHHGLNGGPSPGPYEAAEAFVSRNNCFAIDRSREAFLITWNPKGYLRR"
BASE COUNT        10291 a        12647 c        13177 g         9385 t
ORIGIN      
        1 cgagagcgta aacctgatcg atgatcgctt gccattgaag acgctcgctt atcgactctt
       61 cgcgcaggac atgtttattc gctggatacg tgagatgcgg accgcgatca gaagtaatgg
      121 caacacccgg caactgatca ccgtgggcca ggatgaagcc ggactcggcg acaatcccaa
      181 catgcagttc ttcgcaaacg aaatcgattt cggctcgcta cataactggt gggccaacga
      241 cgatctcgtt tgggacagtg tgctcgcgaa gacgccgtcg aaactcaacc tgatggaaga
      301 gaccggcgtg atgttctacg agaaagctga cggcggtgcg gcgtggcgca gcgagcaaga
      361 cgtcagccgt ttgctcgaac gcaagctcgc gatctctttt gccgcagacg gagccgggtt
      421 catcgagtgg atctggaata ccaatcctta catgaactcg accaacgaag tgggcatcgg
      481 ttttcatcgc gtcgatggca gcgccaaacc ggagctcgag cccttcctgc gaatcgccga
      541 gttcatgcgg cagtacggtc agtatctgaa ggatcgggta ccggagcctg tagccatggt
      601 tattccgcac tcgcagatgt ttttgccgcg cagttttgcg caggaggcga cgcgacggtg
      661 cgtgcgcgcg atgtactacc actgcggcgt gccgatgcag gctgtcggtg agtaccaggt
      721 tcctgaatat ctggaccagg cgaagctgat tatcattccg tcgcccgtcg ttatcaggtc
      781 caagagctgg agtgccgtga accaggaaac gactcggggc gcaacaatcg cgatttcagg
      841 cgcgatcgat atcgacatgc ttccgccttc aagtttttcg acgctgccgt tgaggaatgt
      901 tccggtcgcg caatcggaga cgattagtat tggtggtcgg gaatatttag tgcgatacga
      961 aggggaaaag atgcagcggc tcgaaaaagc cgtggaccaa tcgaaagctc cgccggggca
     1021 gctgttcaat ttgtcttttg gttccgggcg tgtcatgtgg agcccgttac cgctggagct
     1081 cggcgactcc accgctgcac tcgtcgcgtt ctataacctc gcgctttcca ccgcaggcgt
     1141 ctccccgatt tttacagcga caccgcgaac gcccgccgtg ctcatcttgc cgtcgttgtt
     1201 tcgcgacgtg gtgctgtaca cattcgtctc tgaaaccgat cgcgacacgc agctgcaggt
     1261 cacccatctc gaatcccgaa ctcccttccc cgtcaccatc cccgcccaac gcaccgcgat
     1321 ggtcctgctc gaaagaaaga ctggaacgat catcggccgg atgtaacgtg aagcagcgtc
     1381 gcacttgaac gcggctccgt actggttagt ttgaaaatac gccgtaggcg ttggctaatt
     1441 ccagcccagg gttcgaggaa cgagaaccct gggacaacac atcaaagaaa agcgttctaa
     1501 ccctgaaaag ggttcgccaa ttgagaaacc cttttcaggg ttgaaacgaa tatttaaata
     1561 gctaacccag ggctaaaatt agcgaacgtc ttcggcgtta tttcaaactg gggcactacc
     1621 cgatttcgtt gacatcggcc gttgtcacaa attagcctct acccctcgca aacagctttc
     1681 tggaccatcc ttgcaaactc aaggtccggg cgattttctt tccacggtag atcgggctag
     1741 gtggtcacag cattagaact tcgcatttga ataattcctg tgaggtgaac aaatggaatc
     1801 gttgaacctc gacttagtag caatcatttg gaaagtccgc catagtttct ccagttacct
     1861 tgacgctgag atagaaaaaa acttgtcgat cgcaccaaat ttaaaactga tcaccttcct
     1921 tatactcgtc ttgctgatcg acagcgtggt gttgggttgc cgccaaccgg cagctacaga
     1981 cgagaaaaac tcaaacccgt ggcagcttgt ttgggctgac gaatttaact acaccggact
     2041 tcctgatccg caaaaatggg gttacgacgt tggtggtcat ggctggggta ataaagaatt
     2101 gcagttttac accgatcggc gcccggagaa cgcccgagtc gaaaacggcc acctcgtcat
     2161 tgaagcaaga cgagagccct ggcaaggaag ccagtacaca tcagcacggc tggtcagcaa
     2221 gggaaaaggt gattggactt atggccgcat cgaggtgagc gctaagttgc cttccggtcg
     2281 tggtacctgg cccgcgattt ggatgcttcc caccgattgg gagtacggga agtggccgga
     2341 cagtggtgag atcgacatca tggaacacgt cggtttcgat ccggatgtga tccacgcgtc
     2401 ggtgcatacc aagtcgtaca atcactcgat caatacacaa aagaccgcgc agttgggcgt
     2461 ttctaacgca cgcaccggat ttaatattta tgcgattgaa tggacgcctc aggaaattcg
     2521 tgcgttcatt aacaaggaac actacttcac ttttaagaac gaacgcctca caaatccttc
     2581 cgcagacttc gcccaatggc cattcgataa aaagtttcac ctgctgctga acctcgcggt
     2641 cggcgggaaa tggggcggtg ctcagggcgt tgacgacgcg atctggccgc agcgaatgga
     2701 agtcgattac gtccgcgtat atcgagctaa ttagtcgtat tttttgtgcc tctttgtggc
     2761 gaagcggtag tgtattatcc gcgcatgtca aaaacgctga gcctgttcct tctcctgttt
     2821 ttctgtgtca ctccttttgc cgcacagacc accgctgatg attacaacgc cactttcaaa
     2881 ccgctgaagt ggcgatcgat tggtccgttt cgtggtgggc gatcggtgtg cggcactggc
     2941 gtagttggtg atccgaagac ttattacatg ggaacgaccg gcggcggtct ctggaagacc
     3001 gaggatatgg gccttagttg gcgcaacgtc tccgatggtt ttttcaagac cggatcagtg
     3061 ggcgcgattg cagtcgccga gagtgatccg aatgtcgttt acgtcggcat gggtgaacac
     3121 gccgtgcgcg gtgtgatgac gcatcatggc gacggcgtct ataaatcaac ggatgccgga
     3181 aaaacgtgga agaagatcgg gctcgatgcg agtcaacaca tctcgcgcat catcgtcgat
     3241 ccgaaaaatc caaacatcgt atacgtcgcg gcacagggcg cgttgtacag ccacaccaaa
     3301 gaacgcggtg tttataaatc cgtcgatggc ggtgcgacgt ggaagtcagt tttgtatgtt
     3361 gacgacaaaa caggcgcggc cgagttgtcg atggacatga acaacccgcg cattctttac
     3421 gccgcgatgt gggaacacgg tcgtctgccg tggaaagtaa tcagcggcgg tcctggcagt
     3481 ggactctaca agtcgaccga cagcggcgag acgtgggaca agctgaaaga cggattgcct
     3541 gaagagatgg gcaagatgtc gatcgcggtc agccgttcga atccggagaa agtttacgcg
     3601 ctgatcgaga gtgattccga taaagaagcg ggtgggctgt ttgtttcgag caacggcggg
     3661 aagaagtgga gccgcatcac caacgatcac cggcttgtgc agcgtgcgtg gtattacatc
     3721 gagctgttta tcgatccgaa agacgagaac acgatctatg ttttgagtgc ttcggcgttg
     3781 cgttcgaagg acgcgggcaa aacgtgggaa acactttccg gcacgcacgg cgactatcac
     3841 gatctctgga tcaatcctga taacccaaac aacttcatca tctcgaacga cggcggctcg
     3901 gcagtcacct tcaacggtgg caagatcttc agtacgcaga gcaacatgcc gaccgcgcag
     3961 ttttaccgca tcaatgtcga caaccagttt ccgtatcgca tttacgccgg gcagcaggac
     4021 aacacgactg tttcgattgc gagtcgtgag ctgggcggtg gcgggattgg tccatcgagc
     4081 tggatgcctt cggcgggcgg tgagagtgcg tttctcgcgt tcgatccgaa tgacgttaag
     4141 tatgtgctcg gcggtagtta ccagggaacg atcgaggcgc tcgacgtcaa agcgcaggcg
     4201 agcacaaaca tcatggccgc gccgattcaa tatctcggca tggacgcgaa agatatcaag
     4261 tatcgtttca actggaactc accgatcgtc tggagcaaat acgagccgaa tacttatttt
     4321 cacggcgctc aatacgtttt gaagacgacc gacatgggtc gcacgtggaa agagatctcg
     4381 cccgatctca cgcgcaatga gaaggagaag cagggcaaag gcggtgagcc atacacgaac
     4441 gaagccgttg gcgcggaaaa ttacggcacg ctcgcttaca tcatggaatc gccgtacgag
     4501 aaaggcgtga tctggaccgg cagcgacgat ggttttgttt acgtgacgcg tgacggcggc
     4561 gcaaactgga agaacgtcac cccgaaagga ctgcaggagt gcttgatcaa tgcgatcgaa
     4621 gtctcgccgc acgacaaggc cactgcttac atcgccacca cgcgttacaa gttcaacgat
     4681 cacacgcccg gcctttataa aacgaccgac tacggaaaca cctggacgaa gatcacgaac
     4741 ggcttgcctg cgaatgcgtt tacgcgcgtt gtgcgtgaag acgacgggcg ccgcgatctc
     4801 ttgtttgcag gaacggaact cggcttgttc atttcatgga acggcggtcg cgattggtcg
     4861 ccgtttcagt tgaacctgcc gatcacgccg atcaccgatc tgcgtgttca caaaggtaac
     4921 ttgatcgcgg cgacgtcggg tcgcgcgttt tgggtcctcg atgatttgag tgtgctgcgt
     4981 cagtacaaaa aagacgcgcc atcgttcgcg atctaccggc ctgacggcgc gtatctcgtg
     5041 aacggctcga gcgagctcga tcgaacagat aacgagttta cgggagcgag cacgtttcgc
     5101 ggcgtgaatc ccgcgaccgg cgtcgtcctt tattatcaac tgcccgagct gaagaaagat
     5161 gacgagatct cgctggagat caaagacacg tcgggcgcga cggtccgcag ctactcttcg
     5221 aaagctgatg agaagtttgt gacgtacgat tccggaccgc ggcctgatcc gacgttgccg
     5281 aaaggcaaag gtctgaaccg ctttatgtgg gacatgcgtt acgagacaat ggtgggcgtg
     5341 ccgggtattc gtatcgaagg cggttttgct gcacacaaag ccccgcccgg gcgatacacc
     5401 gtggttttga aaatgggcgg acagacagtc acgactgacg ccgagatcct tgcaaacccg
     5461 ctctatccaa ccgacgcttc gacttacacg cagtatcacc agacgatgct cgcgatggaa
     5521 accgagttga ccgcgatgca tcgcatggtc aacagtctct acgaaaagca aaagcagctc
     5581 gaatccctgc tcagttcgct accgagcggc gagaagtaca ccgcgatcaa gaaagacggc
     5641 gaagccctct tgaaaaaaat gaaggcatgg gacgaagaca tggttcagcg gaagtcgaaa
     5701 gcctacgacg acgttgaaaa cttccccaac aagttcaccg ccaactacct cttcctgatc
     5761 aatcaaaccg aaagcgacat cccacgcatc aatcaaccct cgctcgatcg catgaaagaa
     5821 ctgaacgccc aatggacctc attgaaagca cgcggcaccg agctgctgaa tcaggattta
     5881 cccgcgttta ataaacgatt gtgggacgcc ggcttaggcg ctatttggaa agatagttag
     5941 ccacggatta cacggattta aaatccggat ttaaatccgt gtttatctgt ggcccgatct
     6001 acgtgtggtt tacgcgtatg taggtgacaa aagttgttat tgacacgatt cggaaatagg
     6061 catattgtca cgcgcacagt tggaaatttg tacccccaca aaacctgact gccgttgtgt
     6121 gttcctttat cggtgaaggt actcattagc cttcacgctt ctatcaatct aactcttggg
     6181 cgttccccat tgaggttttc ctcgcagaaa gcttcctccg gtgccaggcc tacggtttcg
     6241 attgtttggt aagttcacgg cacatcatga tgccgggctt ttaacaggac ttgaagcgac
     6301 caaggatcag gaactactta gttatctcct tatccatcgc gatcgtcccc actcacgcga
     6361 agcactcgct tcactgctct ggggcgatac ctctacggaa aagtccaaaa aatacctgcg
     6421 ccaggcgctt tggcacctgc atgccgcgct gaactgcgat aacggaaaca attcagaagt
     6481 gctgatggtg gaccacgact ggctcagtct gaatccgtgc agcaacttgt ggaccgacgt
     6541 cgcagatttt gaaagcgcgt ttttagctgc cgagggaatt ccgggtcgac agcttgacaa
     6601 cgagaaagcc cgtggattga aagacgccgt aacgctttat aacgacgacc ttttgcccgg
     6661 ttattaccag gactggatcc tcttcgagcg tgagcgtttg cagaacatgt acctgctgat
     6721 gctcgacaaa ctactcgtgt atctccagtt tcgaggcgag tatgaagtgg cgcagggcta
     6781 cggcgcgacg atactgcgat acgatcccgc ccgcgagcgt acgcatcgac agctcatgca
     6841 cttatattcg cttgcaggcg atcggacgtc ggcgttgcga cagtttgatc gttgcgccca
     6901 cgcgctcaag caagagctcg gagttaaacc ggagaggaaa acagtcgagc tttatgaacg
     6961 aattaaatcc gatcagatgg gccataccga cgttcccgaa aactctcact cctcaacatc
     7021 gcccgacatt cttacccgcc tgaaacagct tcaagcgatc gtgtctggag ttcagcgtcg
     7081 cattcaacgc gacatcaaat ccatcgaaac acttcattcc aacgacaaaa cgagccgcgg
     7141 ataatacgga tttaaatccg gatttatccg tgtaatccgt ggctagagac gatcccgaga
     7201 cgtgtgcgag acgcttggac agcaatgcgt tttgtataac gaaacatgga cttaacagag
     7261 tccaaagttc attcctttat cgtcaagctt tggcttgagg gcgatgctaa ttcgttcgtt
     7321 tggcacgggc acataacaca cgtgccgagc ggcgaacgtc gttatttcaa aaagctgagc
     7381 ggtattactg atttcgtcag cgaatacctc gacggcagga aagcgcagcc ggacctcaaa
     7441 tcgcgcatga aggactggct caaaaaacgc cgagcttaaa agaagatggg aacgtttcag
     7501 gcaatcgccg caacaggaca agccatgttg ggactgctgt ctgacgccgt gccgcgcgat
     7561 cagtttccca acgcgcagtt tgagctctac cagatgagca actttcagca gccgatggaa
     7621 gaaggtatct ccttgtttct ctaccgcatc gctgccaaca cgtcgcgccg gaacctcccg
     7681 ccaaccaccg gccctgatgg acgtcgcttc cgtccaccaa tcccggtcga tctctattac
     7741 atcgcgaccg cctgggcgcc cacggcagtc agacaacaac gactgctcgg ctggaccatt
     7801 cgcatgtttg aagacgtgcc gatactgccg acaggcttgc tcaacaacta cggtccggaa
     7861 cccgagattt tcaaaaacgg cgagacggtc gaggtcgttc tcgattcact cacgctccag
     7921 gacctgaaca atttctgggg cgttagcaaa agcagtctgc aactttcagt cggttacgtc
     7981 gcgcgcatgt tgcacatcca gtcgtcgatg ccgctcacgg aatacgccga agtgcagacg
     8041 cgcgagtttg gagtaggaaa agtttagacg atggcggcac cgttcatcac tctcgatcgt
     8101 ttgaccatgg tcgcgccgct cggtctgcgc tttcacgact cgaccactgg cacgatcgta
     8161 gacgacggtt tggttgtgtg ggcatacccc gtgggacgac cgacagcgaa gcgacaagcg
     8221 atcgccaatc gcaaaggcgt ctacgtgctg caacacggtt acggattacg tgcgagagaa
     8281 aacggcgacg gatcgcgttt ctactgggac catcccgttc caccgaacaa agatttcgta
     8341 atcgaagtca ctgatctgca gcggcgtttt caatcgtttc agttcactgc gagtcttcca
     8401 gccgagggaa ttttcaagtg gctcgattcg ccgctttcca cgcgcacaac cgtgcccctc
     8461 tttcccgcgc caacacaaag cgtcgcgtcc ggaatggctg ttattcgcgc cgacttgtgg
     8521 gacacgtcaa tcgacgcacc cgcagcatgg gccgtgatcg aggcttttac ttcgggaact
     8581 ggaggcaaat tcctcgggcg cggaatcgca gacgacgctg gccgtatcgc gttgatcttt
     8641 gcttttccat cacctttgtc atttgccccg gtatcaccac cgggatcgcc gttgggttcg
     8701 ccgccgatag caacgagccc gccgttgacg gaacaagtct ggccgttaga gctgcacgcg
     8761 ttttatacgc cagtgcgccc ggttgtgtcg ccgcccgatt tcattgaaga agttggacca
     8821 ccactaccag atcttagatt cactttatcg cagccggccg caacgttgtg ggccgatgca
     8881 gaccaaacag aaatattgga aacgaacttg cgattcggtc gcgagctgat cttgaaatcc
     8941 agaccatcac cgtcgtcacc gccttcgccg ctgtcgcaac cgcgtgattc ggtgttgttc
     9001 ataagcccgg cggtctcacc gccataagga ggagtcatgc ctgaatacct tgctccggga
     9061 gtttacgtcg aagagacatc gtttcggtca aagtcgatcg aaggagttag cacgaccacg
     9121 acgggtttca tcggcccggc tcgatacgga ccgctcgatt tggaacccga tatcattacc
     9181 agtttgggag aattcgagcg cacctacgga gaccggcaac agttgaactt ctccgacgct
     9241 gtcggaatgg ataactacct gtggcacgcg gcgcgagcgt tctttgaaga gggcggcaaa
     9301 cgtctctacg tttcgcgcgt gttccgaccc gcggccgacg atgatgacgg gcgtgcaagc
     9361 gcgactttgc cgacatcagg cggagccggc gcggtcacga tcagcgcgcg ctttccaggc
     9421 gaagcaggca acatgcgtgt tcgtttcgat ctgctcgtgg gccagaacgt cctcggcacc
     9481 gacgaaaacg gtgacgcgcg cgtggccgga ctctttgacg gcgatgtcgt ttggatggct
     9541 gacacggcga gcccgatcgg tagtccgctc ggcacgggtc aactgttccg cgcgtcgttc
     9601 ctgcaggaga cacaggcgtg ggtcttccga tcgtcgggca gtcccggtag cgagctcggt
     9661 ctcgatcaac tcaatgccga tggcggcgac gttatccgca ccatcaccgt gagcgtgact
     9721 gtgattccga atgatccgag tacttttccg gaaacgtggg ccgcgctgcc ggtcgagccg
     9781 ggtcaccttc aggccggtat gcctgactcg ctgtcagcga agtttgcggc gcatccgaac
     9841 aacctcgcgc aggcgcgttc gttaccgatc gttatcgagc tcggagctaa cgtcgatact
     9901 gggattgagg tgttaaccgc gctcttcgga cttaatgcta cggacttctc gaatcctgac
     9961 cagccgctgt ttgttgaagt ctctttgact ggcggtaacg acggtgcgcg tcccacagcg
    10021 ggcgagtacg aaggcgaaga agagcccggc aagactggtc tcaagatctt cgaagacatc
    10081 gaagacatct cgatcgtcgc ggcgcctggt tcgacgttcg gttacgagaa cggctatcgc
    10141 gccgatgcaa acacgatcac caacctgctg atcactcacg cgagtcgtat gcgctatcgc
    10201 atcgccgtgc tcgacagcgg caacgctcaa agcatttcgc aggtccgcgc acaacgcgcc
    10261 cgcttcgatt cgacgtacgc ggcgctttat tacccgtggg tccgcgtgct ggatccggtt
    10321 acgcgcaagg agatcaatct gccgccgagt ggattcgtgg ccggcatcta tgcacgcaac
    10381 gacatcaatc gggcagtcta caaagcgccg gcgaacgagg tcgtgaatct cgcgctcggt
    10441 tttgagaagt tgctcaacaa gtcgcaacag gacgttctca atccggaagg catcaactgt
    10501 ttccgcttct ttgaaggccg cggtttcaga ctctggggcg ctcgcacgat cagctccgat
    10561 cccgagtgga aatatgtgaa cctgcgtcgc tacttcgctt acctcgaaca ctcgatcgac
    10621 aagggcacgc aatgggcggt cttcgagccg aatggagaag cgctctgggg caacgtgcga
    10681 cggacgatcg aggatttcat gctcaacgag tggcagatgg gtgcgctctt gggcgacaag
    10741 ccggagaaag cgttcttcgt gaagtgtgat cgatcgacga tgacgcagaa cgatctcgat
    10801 aacggaaggc tcgtttgcct catcggcctc gcgccgctgc gccccgcgga gtttgtgatc
    10861 ttccgcatcg gccagtggac cgcggatcgg aaggtttagc agttggattt ctctgcgcag
    10921 cctctgcgtc ctctgtgcct ctgtggttag agttaagaga tcaccgcaga gacgcagagg
    10981 acacagaggt aacgcagaga agaactaaga tcttaaggag gactttatgg cagttctacg
    11041 cgatcgccct tacgttcaat tcaactttct cgtcgacctc ggcacgggca acacggatgg
    11101 tccggaagca ggcttccagg agatcagcaa catcggtatg gaagtcacgg tctccgagta
    11161 tcgcaacggc aactcgaaag agaacagcgt gcgcaagatc acgggcctga acaaagccac
    11221 cgacgtcacg atgaaacgcg gagtgatcgg ctcactcaat ctctaccagt ggctcaacga
    11281 cattcgcaac ggaaatcaga atgccgagcg gaccgtcacg atccagttgc aaaacgaaga
    11341 tcacacggcc gtggtccaaa cctggaaact gctgcgcgcg aaaatcatta agcacgtcag
    11401 tggcccggtt aacgccaagg gaactgacgt cgcgatggaa gagctcacac tcgcctacga
    11461 acgactcgaa atggagtagg taaatgagtg cgcgcgcgcc gagaagattg cccgggtttc
    11521 gcttcgaaac tcaggcgcca ccattgcccg aggttctgcc tcgcatggac atcgccgtgt
    11581 ttgtcgggtt cgctgcgtct ggaccattgc aaacaccggt ggtcgttgag tcggaagcgc
    11641 aattcacggc aatcttcggt gacgacacgc cactcgcgtg ggacctcgtg cgtggtgaag
    11701 agattcacgc gcaccttgga ccagcagtgc gcgcgttttt ccgtaatggc ggacagcgct
    11761 gttggatcat tcgcgtcgca cgacaaaccg cgagtgcacc tcaaccgctg aatcgcgccc
    11821 gctacaacta ctttccgctt ccctacctcg cgcgtgcggc gttcaacccg aatgggcaga
    11881 tagctagcct tacaccggcg tttgcgcgtg gaagatcgga aggcagttgg tccgatgcgc
    11941 tgcgggtgag cacggcgttg ctgtcgcgac cgatccaggt caacggcgct ttgctgcagg
    12001 acggtgttgg ttacgcgttg tcgatcgtgc gcgatccttc gaatccgctc gcggcgggcg
    12061 acatgatccg gctgacgtac gagagcgcag gtgtttcggt gttgcttgcg ttacagacga
    12121 tcacgagcgt gttgtcgagt ccgttaggcg cgccgttggt gaatgcgact gtgagtcgag
    12181 ctgtttggct gttatcgatg tcaagcgatc cgagtgcacc gtcgccaaac actccggtga
    12241 ccgcggccgt gttcacacgt gagcagctca gctcaccgcc gaacccagaa gacgacatcg
    12301 ctgcgtttga gattacgtac aatgcggtgt tcgtagacaa aaagatcacg ctcaagctgc
    12361 tcgactgccc gctcgctgac gcaccaccgc ccggctcgat tgtcaggatc gatcagggcg
    12421 gcaatcactg gtggatgacg gtcgatagcc tcgatttcac aagtggcgat gaaggcgtgc
    12481 cggtgttgtc tggcagcgcg tctcgcatca tcgatccacc gaatccgtta cccgttgcga
    12541 caccggccgg cgagcgtctc agcttcgaga tctgggtgcg acaagctgaa gagtattcga
    12601 tcagcctgag cgatctcggc ttcgcgccgg atcacgagcg cttctgggca ccgttgccaa
    12661 cggatgaaga agtctatcgc ctgactgaca cgcccacgac tgagaacccg gcaacgttgc
    12721 tttggaaaca ggttggcgat ctgtttcgtt tcccactcgc cggacttgga ccagccgacg
    12781 agatctactt tccgttgttg atgccgagcc tggctgaaaa ctatctcggt ccggtcgcgc
    12841 tgcctggcag cgagagagaa cgcgacggac ttgcagagtt tgatgcggcg ctgtttttag
    12901 atcccgatct cgttgacatc ggcgccgcaa atctcgcaac cacggccgac tttcttcagt
    12961 atctttcacc gcgtccacgt cgattgactg gcatgcacgc ggcgtttcca ctggaagaag
    13021 cgacgatcat cgccgtaccc gatgcagttc atggtggttg ggtgaagcac gagcgcgaac
    13081 cactcatcga tccacagcca tcaccgccgc cgctgcgtcc cgagtggtgg cactttctcg
    13141 attgcaatcc ggcgccgaag aaaacaccag cgctcggcag ttgcgatcct gagccgcccg
    13201 agccgtcgcc aatcaaaccg gtgcacgagc ctgagtgggg caacttcctg agctgctcga
    13261 tacagatcat cgaaccgcct cagttgttcg cgttcccaca gttcagcagt gatggaaact
    13321 tatcgctgcg ctgggagctc tcgccgccac aagaagcgca ctacgtgctc gaagagtcgt
    13381 cgcagccaaa cttttccgac gcggtgacag tcttcgaaga cacgacgagc agctttacgc
    13441 tctacggccg ccgcaccggt gattactact atcgcgtgcg cgcgatcatc ggcgctgaca
    13501 ccagcgactg gtccaacggc gttgcggttc gcgttgaaga cgaggagtcg cgctggatcg
    13561 tagacacaaa agactattcg cctgatgtgc tgcttgccgt ccaacggtcg ttattaagat
    13621 tttgtgcagc gcgcggcgat atgttcggcg tgttgtcgtt gcccgagcac tatcgcgaag
    13681 acaaagcgat cgaacacacg agcctgctgc gggcgacgcc gaacgtcgtg ccaccaaccg
    13741 aaggtgtttc cgcgctcagt tttggtgaag tcaacgtgtt cagttacggc gcggtttatc
    13801 acccctggct gatcggtcgc gagaaccaaa acgatactgt ggtctcgatg ccgccgtgcg
    13861 gcgcggtcag tggctcgatc gccgagtcgg ctttgactcg cggtgcgtgg atcgcgccgg
    13921 ccaatcgtcc gctgcgtgcc atcgtcgcgc tcaagcccac cctacttccc gagagacgtc
    13981 tcgaacttca ggacgcactc gtcaatatcg tgcgacagga accgcgcggc tttctcgtgc
    14041 tcgactctga cacgttgagc gccgatgaag acttgcagga gatcggtgtg cgccggctgt
    14101 tgattctgct gcgacgccag gctttgcagc tcggcgtcac ttacgtcttc gagccaaaca
    14161 gccccgcgtt tcgtcgcgca gtagatcgcg gtttcacgga aatgctcgat ggaatgtttg
    14221 agcgtggcgc gtttgcgggc gcgaatgcgg caacgtcgta ccaggtcgtc actgacagct
    14281 cgctgaacac gccgcagagc gtcgatctcg ggcgcttcat catcgagttg cgcgtggcac
    14341 cgtcgctgcc gctgcgcttt ctgacgattc ggctggtcca atcaagcgat cgaacgcagg
    14401 cattggaggt gagatagctc gtggccaatc ctgaaaagga acaagttgcg tatccgttca
    14461 ctgcgtttaa tttcgcggtc gagatcaagg ttgacggggt ggcgatgcag gttgtcgatg
    14521 ccgcgttttc cgagtgcgac gggttggaaa tgacgatgga cgtgaagacg attcgcgaag
    14581 gcgggaataa cggcaggcag atccgtctta ctggtccact cacgttcgga caagtgacga
    14641 tgaagcgcgg tatgacttcg aatctcgagc tgtgggattg gttcaatctc atgctcagcg
    14701 atcaatcgct gcgtgcagat gccgaagttg tgatctttgc ctccgatggc gagacggagc
    14761 gcgcacggtt tcagttgtcg cgctgcgtgc cggtgaagtt gaagtcgccg gcgttgaatg
    14821 cgaaggatgg tgcggtggcg atcgaagagt tgcagatcgc ttacgagaaa ttgacgttga
    14881 aaaaaccgtg aggacgcgaa gatgcctggc tcacagaaac tggaaaaagc agagcttcga
    14941 gagctcgacg ccgagtttaa gaacgaaatc aacaccgaca agaagtgtca ggtgcagttc
    15001 aatcctgaga cgctgaaggt cagctttgcg aaccaggtgg cgacgccatc gggcgccggc
    15061 gatcaaaaag gcacgccggc gcggcagttt gtcggtgcgg gcacgacgaa gctgagtttg
    15121 cagttgtggt ttgacgtcac cgcaccgatg ccgccggaac agaagaagga gcaggatgtt
    15181 cgcaagttaa cggcgaaagt cgcgtacttc atcacgccga aaccggaggg cgacaagttt
    15241 gtgccgccgg ctgtgcggtt catctgggga tcgtttcagt tcgacggaat catggaatcg
    15301 atggaggagt cgctcgagtt cttttcgagt gatgggcggc cgttgcgcgc gagcgtgacg
    15361 atcaacttga cgcagcagaa gatcacggag ttcacgttca gggcgacggc tggaccaggt
    15421 gcaaagccgt ctccaggcac gcgaccattg acacccgcgc cgcaagggaa gtcagtgcaa
    15481 agcatggccg acagtcaggg caaaggcgac aactggcagg acatcgcgtc ggcgaatggg
    15541 attgaaaacc cacggttgtt acagccggga cagttgttgg atctcaacgc cagcgccagt
    15601 gtctcggtcg gcggatcatt ccaggcgggc gggtcaatca gtatttaagg acaggagttt
    15661 caaatggcag ttgtagttaa cgaagttgaa gtagttcctg ctgagcgcgc ggcgccggcg
    15721 gaacaggaga aaggcggcgg tggcgagcag gcgcagccgt cggagcacga gatccgccgc
    15781 atggttgaac aacagatgag tcgctgcgag cgcgtttggg cctactaaag aacaatggct
    15841 gacgagacta caggactaaa agaatctcga cccacggtcg tcgttaacaa cgaagtcaac
    15901 ggcccgctcg gtggcggact actgacgctg atgatcgtcg agaacaccaa cggtctctat
    15961 cgctgtgaag ccaaattcgg caactggggt gagaaaaaca gcacgaccga ttttctctac
    16021 ttcgatcgca gcgtgctcga attcggcaaa gacttcgaga tcaagctcgg caacgattcg
    16081 atcttcaaag gaaagattat gggactcgaa ggtaatttcc ctgaaggaca gtcacccgag
    16141 atcagcgtgc tcgccgaaga ccgttttcaa gacctgcgca tgacgcgccg cacccggacc
    16201 ttcaccgacg tcaccgacgc cgacgtcatc aagcagatcg ccaccgatca cggactgacg
    16261 ccggaagtcg acctatccgg tccaacttac aaagtgctcg cgcaagtgaa tcaaagcgat
    16321 ctcgcgttca tccgcgatcg cgcgcgctcg atcgatgctg aagtctggat gtcagacaag
    16381 acgctccacg cgaaagcaca caccggccgc aacggcgaca agctgcagat gacttacggc
    16441 aatcaactgc gcgaattcaa cgtgctcgcc gatctcgcaa tgcaacgtac gagcgtcgca
    16501 gtgaacggct ggaacatctc gaacaaagcc gcaatgcagt ttgaggcaac tgactccgcg
    16561 atcagcagcg aactgaatgg tgatacgagc ggcgtcagca ttcttcagtc ggctttgggc
    16621 gaacgcaaag aagcactcgc gcacacaatc ccgctcaacg atcaggaagc gcaacacgaa
    16681 gccgaagcgt ttttccgaat gagcgcgcga cgttttgtgg tcgggcacgg gattgcagag
    16741 acgaacaagt cgttgcgcgc tggttcgcaa ctcgacttgc agggacttgg accactcttc
    16801 aacggcaagt attacgtcgc cgaagtgaag cacatcttcg acagtaccaa gggtttgcga
    16861 agtgagttcc gctcggaacg agtcgggatt gggaaatagg taatggaaaa gccaccactc
    16921 gaaatgatcc tcgatgtgcg tcttcccagc ggccttggcg gtcgctggta cggtgctttt
    16981 cccgcgctcg tgacggacat cgtcgatccc gaaggactcg ggcgcgtgaa gattgcgctg
    17041 ccatggtcgc ccgacactgc gggcgcgcgt tacgagacgt gggcgcggct cgcgacattc
    17101 atggccggaa acaatcgcgg tagctggttc attcccgacg tcgatgacga agttttgatc
    17161 gtgttcgaag gcggcgatcc gcgacggccg tacgtgatcg gcagtctttg gaacggcaaa
    17221 gataagccgc ctgagtcgat ggacggggcg ggcaagaact tcaagaaagt actgcgctcg
    17281 cgcaacggcg tgaagctgac gatggacgac accgacgggc gcgagcaatt cattctcgaa
    17341 acgccaggcg gccagaagct gtcgttgaaa gatggcccag gcgcagtcga gatcgtcgac
    17401 agcaacggca actcgatcaa gctggaaacg agtggcatca cgatcacggc ctcagcgaaa
    17461 gtgacgatca acgcgtcgca ggtcgcggtt tcggcgggca tggtgaccgt cgatgccgcg
    17521 atgtcgaagt tcagtggtgt cgtgcagtgc gacacgatga tcagcaactc ggtaatcagc
    17581 gcatcgtaca cacccggcgc aggcaatatt tggtgagtat gagtacacaa aaagcacaaa
    17641 ggaacgcaaa aggcacaaaa agagccatga aattttctga tcggctttct tctggtccgc
    17701 tttcttctga ttccggttct tccaatcagt ttttgtgcct tttgtccttt tgtgcttttt
    17761 gtgtaccccg ttttgtggct aaaaataatg gctaaggatc aagttcaatg ggcagcaccc
    17821 tcgccactct ggcccatcgc ggccggagcg agcgacatcg ctttgcgtcg taacgcgctg
    17881 cgcaagccgg tgatcctgcg cttcgctgct gacacgttca tggacgactt cgtgaacctg
    17941 ctcgaaactg atccttcgcg aatgcacgag ctcgttgcga ctccggaaac gtggcgtgga
    18001 ccaacagccg agcctgcgcc cgcgaaacct gcaccactct tcgcacgcga acttagccgt
    18061 cttggtctcg ccgcggcgaa gaaaaaagga ctcgtgcctt caccgagcgg caaaggtgtt
    18121 tcgtcactgc tgacactgaa cactgccgcg caaccgaaga agctcaagct ctaccagcct
    18181 gcgcaccagc gttattacct cgtcgcctcg tcgctcgtct gcggtcgggc cggtctaccg
    18241 gatcgtgcaa tcaatgccgg ccggcaggag aaggtcggct acgtcgtgcg ccggatgttt
    18301 ccgcctggac cactcgacat caacaccccg ctccccgcct tcgatcccgc gacctgggaa
    18361 gagtacgcgt atgttgcaac tgcaaccggc aatcgctggc agcgaattcc aaagcaaagt
    18421 gatggtctgt tgatcgacgg cgaggatcag ttgccactct ttgcgatgaa cttcacagaa
    18481 gacgacggcc gcggtcgacg tgttgttgcg ggactcgttc cggttggaaa acgtgaagct
    18541 tacatgggcg ccggcatgaa gcaacaggct ggcgatccgg cgccggtggt cgagccgcaa
    18601 ccgatttccg atccgcgcat gatcctcttc tggtcacagg tgacggagcc gtggaagaaa
    18661 ctgctcgaac aggccgccaa cggcagcaag atgaacaagg gatcgaagat tcctgacgat
    18721 ccgaacgcag cgccaccgcc aacgttaaac ctgaacctcg ataatactgc ctcaacagca
    18781 caaaccctgc gcgagcagat tcagaccggc tcgtggtatg tgctgctcga cttcgcaaac
    18841 ttgctcatag agcaaacacc gcgtgtgtgg cgtttgctta atggtcaggg accacccgcc
    18901 ggtgagcccg cattcaatgc tgccgagaat gcgctcgcca cagcgatcac gggcacgact
    18961 ttcagtctga cggattcgca gaaagatgtg tttcgcgcaa acacggctta ctcgcggccg
    19021 gcgatcaaga gctcgttgaa agacgcgttg ctcgccgtga aaacaggatc gatcgaagcg
    19081 aaccttgaag cagtcaagac gtcgttcgat cgcaaaagag cctcgctcga tccgttgtgg
    19141 cccgactttc tgtttccact cgctgatcca ttgctgccga gtccgatgac gaccacggct
    19201 ctgttggacc aggcgaaaga tagtgttgat gcactcgccg tgaagattca ggccgcgctg
    19261 ccggcggcgc ccgttgctga agttcccaac aacccgcttg tatcgcagca gccgatggac
    19321 atgcgcgaag gatggtttgt gattcgctgc gtgttcacgc ggcctgagtg tgggccgatc
    19381 gatccgccgt tgctgagcga gccgacagcg ccgtttcaga tggccggctt cttcgatccc
    19441 gatgcaccgg cgcgcccgat ccgcatcgct ctgccactcg atacgtcgcc ggcgggtttg
    19501 cggaagtttg ataagaacac ggcgttcatg atctccgaca tgctttgcgg gcaaatcgag
    19561 cgggtgaagg gactgtcact cgcggatctg gttttgtcag tgttgccgtg gccgctgcac
    19621 aaggatctgt ctgtgccgga tggtggtcca tgcaagagcg actcgggaat tgaattcgga
    19681 atgatctgtt cgttgtcgat cccgatcatc acgatctgcg cgctgctgtt gttgatgatc
    19741 atcgtcttct tgctcgacct cattttccgg tgggtaccgt tctttatatt ttgttttccg
    19801 ataccaaaac taaaggcaaa agcgtgagta cacaaaaggc acaaaaggac aaaaggcaca
    19861 taaaaatttc taacaagcat ttttgtgctt tttgtgcctt ttgtggctaa gaggatctta
    19921 tgaatgcagg gcagatattt gggcgaggaa taagttttcc accacgcgtg ggaccggatg
    19981 ggcgtgtgtc gtggtcggag ggtgaggcga atgtgcgtga gtcgattcgc gtgatcctga
    20041 tgacggaaca gcctgaacga ctacgaacgc cgaagtttgg cggcagtctc gggcgtttcc
    20101 tcttcgagcc aaacacggtc agcacgcgtc gctcgatcca ggaccgcatc acgaaagagt
    20161 tggcccagtg ggagccgcgc atccgcgtcg agtcagtcaa cgtcgatccg gatccgtccg
    20221 atccgcaggc cgcgatcgca acgattattt acaagctggt agcaacacag gccgttgagc
    20281 gcgtcagtgt aggcgtgagt ttgggagcat aacaatgcct ctacaaatcc catccattga
    20341 tgatcgtcgt taccaggatt tgctgaacga agcgctcgcg cgaattcccg ttcacaatcc
    20401 cgagtggacg aatttcaaca agagcgatcc gggcgtcacc ctcctcgagc tcttcgcgtt
    20461 tctcactgag aaccttttgt atcgctcgaa ccagatcccg gagcgaaacc ggcgcaagtt
    20521 tttgcaactg ctcggcgtgc cgctgcaacc tgcgtcgtct gcgcagggcc tcgtgagttt
    20581 caataacgat cgtggactgc ccgagacgat cacgctcaac agcggactcg aggtgcgcgc
    20641 gggccaggtt cccttccgca ccgatcacgg cctcgacgtg ttaccgatcg aagccgccgt
    20701 ctactacaag caaaggatcg tcagccctga caccgccgtc actgactact acaaacttct
    20761 ttacgcttct tataacggac agccgccgaa cgatacggaa atccagctct accagagcgt
    20821 gccgatcgac gggcgcgagg gaaacgaagt cgctattggc caggacgcga ccgatcgctc
    20881 cgtgtgggtt gcgttgatgg tgcgctcgac cgacaagccc gtggacgata gcgagggcgc
    20941 aaaagaggat ctcagagata aggttcgcaa agcgattgcg ggtaagacga tcagtctcgg
    21001 gattgttccc gcgatcgagg ccgctagtcg cgtgctcgca cccggcggca gcgcgaatcc
    21061 cgaaggcgag ccgctgcttc aatatctgat tccgaatgtt cgcagtgatg gtctgttgcc
    21121 gaccgatccg gcgcagcgtg tgccgcagta caggccgttg gttgcgagtc cgctcggtaa
    21181 cgtgctcgag atgcccggtg ttgttgagat cactttgcca tcagccggtg agctgaaact
    21241 ctggtccaat ctcgatccgc tcgaacaggg tgttgccgat tttccgccgt cactcgaaga
    21301 cagtcgcctg agcgatcgcg tgatcacgtg gctacggata caagcgtcgc ccggcgtcgg
    21361 cgcgcgactg ctttggatcg gcatcaacac cgtcacggtc acacagcgcg cgcacatcac
    21421 gaacgagctg ttgacggacg gcactggcgc gcccgatcag gtagtggtcc tttcgcgcaa
    21481 accggtgatt ccgaattcag tgctactgac tgtgagtccg cccgcgagtg acacgcagac
    21541 gtggcaggag gtcgacgatc tgctggcggc aggtccggaa gtgccggtgc cggatctgag
    21601 gctgccgcct ggcgccacgc ctgtgccgcc gctgccgtct gaagtttatg tagtggatgc
    21661 tgagtcgggt cagatcaagt ttggcgacgg cttgcgcggt aaacgtccgc cggctggtgc
    21721 gaagctgcgc gcgagttacg actacggtgt tggttccgcc ggcaatgtcg cagccacgtc
    21781 gattaattcg agtccggctt tgcctgcggg catcaaggtg acgaatccgg ttcgcacctg
    21841 gggcgggacg gaagctgagc aagtcagcga cggcgagaaa cagatcgcgc gctacctgca
    21901 acatcgcgat cgactcgtga gcgccgatga tttcgagacg atcacgatgc gcacgccggg
    21961 tgttgatatt ggacgcgttg aggtgttgcc cgctttcaat cccgagctcg tgccgaacga
    22021 agcgggcgat gcgccgggcg cggtgacgtt gctgatcatt ccgcgtaacg atccgaacca
    22081 gcctgatgcg ccggttgcag atgcgatctt tctcgacacg atctgccgct atctcgaccc
    22141 acgacgatta gtcacgactg agctttacct gcgtggtgcg gtttacaagc ctgtttggat
    22201 ctcggtgggt atcaacgtcg tcgccggtgt tgcgactgcc gaagtgcgtg aagcagtcaa
    22261 acgcgcgctg ctcgactttc tcgcaccgat ctcgagcgtg agtggtctaa ctgacagtct
    22321 gacgtcactc acaacacccg cttacgcatc agcgcaaaat ggctggccgc tgtcgaaagc
    22381 cgtgacggcg cgcgagctgc tggcggtggc gagtcgtgtt tccggtgtgt tgctcgtcaa
    22441 cgatgttttg atcgcggaag acacgaaacc gtcgagcgat cagattccga tgaatggcct
    22501 cgagctgccg cgcgtgatgg gtatcagcgt gagtatcggc gatccgatgg acgtcgattc
    22561 gttgcgcggg caagcgccgg ctgctgcggc tccggcgacg cgaattgttc cggttccggt
    22621 aattcccgag gagtgttagc gaagcaatgg acgccaacgg cactcgattt catctcctgc
    22681 taggtcgcga cgactggggc cagtgcaccg tcgacacggc ctcaggaaaa aaatctttaa
    22741 aagagctgtg gggcctgttg ggttcggaac ctggtttgtt ttggaacgaa gagcgtaacg
    22801 agctgacgct tgaaccgcgt ctgttcaagt tcactgctgc gccgaaggac acgtttccac
    22861 tgctcgagaa tcgacgtggt gcggggcgcg atcgttacgg caactggttt tggatcgacg
    22921 aaacgtcgct gaagattcgc gtgcgctcga ttgggtcagg actggtcaca gacttctggc
    22981 cgctcgagca gaagtgtctt gcgccgaaat caaaaggcga tttcacgcct ttgaatgacg
    23041 cagcaccagc cacgctgctg aagctgagcg gtctagcgat cacggaagat cattacctcg
    23101 tcatcggtgt taccgaaccg gccgggctac tgatcttcga tatgcacacc ggccaggaac
    23161 cgcgccaaat actttggccc agcagcgtgg actttgagcc gtttgatatg gcgccgcgac
    23221 cgggcggcgg cgtctacatt ctcgatcgtt tcaataagag ttactgggct ctcgatcgac
    23281 atttcaacgt gatcggtccg gaagaaaacg gcgaaacgat ccttgccgaa ccacgcgaag
    23341 acgacttcca gccggtggat gagagtgaag tgcgcggcac gaagcgtcgc gtgtttccga
    23401 gaaatttttc gctgctgcaa tcaccgctcg cgctcaacga tccgatcgcg atcgaagctc
    23461 tacccgatgg gacggtcctt attctcgatt tcgatcccgg acacagattt tcacggattt
    23521 ttagatacgg atttaatcaa caattaagcg atcaattaac tactgaagcg atcttaagtc
    23581 tgatcgaaga ggacgcgcag agtgagttca cgctcgtcgg gtacgacttt gcggtggtga
    23641 ctaatcgtct gtacgtcgtc gcggcggatg ggaatcagag ttatgtgttt cgcatgtgcc
    23701 tgcgcgatga gaaagttgag ctgcaaccga ttgcggagta cttgccgatg cgactcttcg
    23761 gcggcaaggc actcgccggc acatctgacg gcgcgtatta cgattttgct gaagactgga
    23821 tcccgctcgt gcagcaacgc cggccgcgtt acgtgcccga agcgacgctc gagacgccgc
    23881 tcaaaaccgc cctcgacaag ccttacttcg acagcggcga gactgactgt gtttggcatc
    23941 gcatcatgct cgacgcgcac attccacccg acacgacgat cgagatctgg agccgcgccg
    24001 caaatgaaga gatcgatctg ccgctcttgc cgtggcaacg cgagcccgcg ccgtatttgc
    24061 gaggcgatgg ttctgagatt ccattcctgc gcgcgactgt ttcaaacgcg tgtgtgggtg
    24121 tggcaaacaa aaagatcggc gcgggcacgt gggagctctt gtttcaacgc gcgcgcggtc
    24181 gttttcttca gctacgcatt cgcttgaagg gcgacgagcg cagcacgccg cgtcttcgcg
    24241 cattgcgtgc ttactacccg cgcttctctt acctctcgaa ttatttgccg ggtgtttatc
    24301 gcgaagatac gcagtcggct tcgtttctcg atcgttttct gtcgaacgtc gaaggtcttt
    24361 acacgtcgct cgaagacaag atcgctgccg cgcaaatcct gttcgacgtg cgtagtgcgc
    24421 cgagcgaagt tctcgactgg ctcgcgggtt ggtatggcgt ggcgctcgat ccgagctggg
    24481 acgaagcgcg tcgtcgcatg ttcatcgcgc acgcgatgga cttcttccag tatcgcggca
    24541 cgattcgcgg tctgacaatg gcactgcacc tcgcgttgga ccaatgcgct gacgaaacca
    24601 tctttgaagc tacgagacca ctcgagccga tccgcatcgt cgagcgctat cgcacacgca
    24661 cactgccggg cgtgatcttc ggcgatccga ctgagtcaac aggaccgcgc gcggtgaaca
    24721 ccggcacatc gtggcgaccg gatcagggac gcgcaaatct caatcaacga tatctggatg
    24781 cgctcggatc tacggcgaat ccgctgacgc aatatccgct cgttccgccc acagacgcgc
    24841 aggaaaacgc aacgtggacc cagttctcgc tagcaacgct cgggtttgtt ccctctgcgg
    24901 ctgtgcctgg tcccgggaag cttcaactct ggcaggactt tctcgcccgt cgatatcgac
    24961 gcatcaagtc gctcaacacc gtctatcaaa caacgtacac cggctttgaa tcagttccct
    25021 tgcctgacca attgccattc gacggcgcac cgctagccga ctggtttcaa ttcgagagcg
    25081 tggtgttgcg catgcaaagc gcggcacatc gcttcaccgt gttgctgccg gtgcctttgt
    25141 cgctggcttt caatcccgag gaacaccagc gccgtatgga tctctcgcgc cgcatcgtcg
    25201 aattggaaaa gccggcacac accgtttttg acgtgaagtt ttactgggcc atgtttcgca
    25261 tcggcgaggc gcggctcgcg ctcgacactt tactcgcgca aggcagtcgg gcgccgcaac
    25321 tattgcccga gctcgtgttg gaccagggct tcgtcggcga gagttacctc ggcgcaccgg
    25381 tacctgaaaa cgcttgcgat cgtttcatca tgggacgcga tccactccag aactcctagt
    25441 gaggagatat aaatatgagt tgcagttgtt tctcatcgaa cacaaacagc atgacgttgg
    25501 ccgagccgaa cccggccaaa catgtgaact tcaacgtcgg catggttctc ggtgtcgacg
    25561 acttcacgca ggagtttgcg tacctctcgg gccgcgacca gtggctcgcg cgcgatctga
    25621 tcggctacgg caccgtgcgc ggcttgaacg tgcggattga agtcgacgcg attaaaggcc
    25681 cgcgggtggt ggtcgaagcg ggcgtggctc tgaacccgcg cggccagttg atctgtgtgc
    25741 cggcggcgca gtgcgcgtat ctgaaagact ggatcgccga tcactctgca gacatcacgc
    25801 cgcaggtcag ttcgccgccc gattcggatc ttcagttgta cgtcgtgctc tgttatcgca
    25861 actgcccgac tgacgatgtg ccgatcgcgg gtgagccgtg tcgcagtgaa gacaaactga
    25921 tggcgccgtc gcgcttgagt gacgacttcg tgctggagct gcgtctcgag agaccgaatc
    25981 aacgcgagga agatgcggtt cgggatttca tggcgtggtt gaagcaggtc cacatcagcc
    26041 aggtcgatcc ctcgacgccg ttggaccaat ttctccaggc gatccgcgat gcggcggctg
    26101 tgtggctcgc atcgccgctc tcgtcaccgc cgggcgattt catgtttggc tcgccgccgg
    26161 gatctctcgt gatcaatgcg gctgatgtga gcgaatacct ccgcgcggcg tttcgtgttt
    26221 gggtgacgga gctgcggccg cgctggattg agcgctggca tggttgtgcg gcgacgcata
    26281 tcgaaggtga tgcggcaggt gatgaggatt gcgtgctgct ggcccagctc gacgtgccgc
    26341 tgctgccgat ctcgccgggc acgtttgata ttcccaacgc gccgattccg gtgaatcaga
    26401 atgatcggcc gttcatcgtg cacttgagga tgttgcagga gtggatgttc tcaaatctgg
    26461 cgatgacgat tagcgctttt accggtacgg gtggtggcgg cggactggac ttcgacgtcg
    26521 tgtcgctgaa gccgccgcag ggcgcgccga tctcaaacgt cgatggtcca atatcgttcg
    26581 agctcaagga cgagcaaatc gtcatcgcca acagcaccaa cggagtggtg cgaatggtgt
    26641 taccgccgac ggcgggccag gacgggcggc tgatggttat caagcgcatc agcaccggct
    26701 cgcaggtgca gatcggcgcg aacgcgggcg atctgattga gggacaagcg tcgctgatcc
    26761 tgacggcaca gaaccggttc gtgcagctcg tggcgaatga aaagctgaag acgtggcacg
    26821 tgatcgcgca gtaagaaata ggacctatag ggcctatcgg acctattagg aggaaacaag
    26881 catggattca cagatcagtt tgcaacagcc gatcctcgac ggtggtatcc gctcgatcaa
    26941 tttcttcaac gggcgtctgc tgagtgcgcg tgatctgacg cgcgagcagt cggccaatcg
    27001 tgaagcggat cgacgtttag gccaggcggt cggtgagggt attgcttacg gactcgaagt
    27061 ctcgaagtcg accgcctcaa cgcaagaatc acccgtcatc acagttgaag cgggcctagc
    27121 gatcaatcgg ctcggccaga ccctgatgct cagcgcgaag acggaagtcg cactcgttcg
    27181 caaagcaact ccaggtccgg acaacacaga cccgtttagc gaatgtgtgc ctcttcagtc
    27241 aggcacgtac atcgccggcg caggtgttta cctcttgacg atcgcgcccg cgcaaggcac
    27301 tgaaggccgc gcctcgacac acagtctcga aagcacgaac gcatcctgca acactgacac
    27361 caacgtcaac gccgtgcagt ttcgcttgat ccaactggat caacaaatct ctgcgcagga
    27421 gatgttggac cagacgaagc tccgcaatct gatcgcgtac aaatgcttcg gcgtcgacag
    27481 cacgagcaag tttccggtga atccattcac tacgaatctc gaaaagtacg gactacttga
    27541 cgatctccgc cccacgtggc tgacggaatg cgaagtgcca cttgcgattc tctactggac
    27601 gctcgtcgac ggcatcaagt tcatcgatct gtggtccgtc cgacgccgtc tcgcacgccg
    27661 tctcacagac aagacgtggt cgtttctcac ttccgagcgc accactcgcg aaggcgaagc
    27721 gatgtatcta cagtttcaga gtcaactgaa agatctgcag actggcggcg tcatagcttc
    27781 acaagtcaaa gccagggatc acttcaaata cttgcccgct gcgggtctgt tgcccgtcgg
    27841 cggcaacggc ggtttcgact acacgacttt cttcaacgag atcacgattc gccaaccgat
    27901 ctttatcgaa ggtgcacagc ttcgctcttt atttcaggag tcgtttgagt atccaccggt
    27961 cgagacgagc agcgagcttg ttttctacct gtacatcgtg cgcgaaaatg cgcagtcgtc
    28021 gctcggaggc gcactgtcca cgcaaagcta cgtggtcttc gcaagcggcc acacgcttta
    28081 tcgcggcgag gcgcgttatg acgttcatca ctggaacttc ggcaactttt cctaaaggag
    28141 tatcgagatg gcccaactac ttaatcacgc tcaacccggt gatgtgatca cggctgacga
    28201 ctggaatctg gtcgttgatg cgataaacga gctgttgcag gcaggccaga caagcggcat
    28261 caaaatagat aatgtcgtgc cggccggctc cgcgctcgat ccgttcaaga tcggcagcct
    28321 ggtccaggtt gtcgggcaga attttggctt ctcgatcggc cagtcgaaag tcatcttcga
    28381 ggctggtgga acgagcgtaa cggtgctgcg agcacagatg ctgggaggct cattcgacga
    28441 gcgtttgatg tttatgatgc cgccgattcc agggatcacg ccggcgggtt tggcaatgac
    28501 gatgaaggtc gacaacggcg tcgcgagtga taatcgatca gtgttcgtga tgccggtggt
    28561 aatcagcttg atcggcgata ttttcgtggg cttccgcgcg gacgttaatc cgaacccgag
    28621 tcccaatccc gttgtatcca cgcaacaggc ggacttcgct ttccggctac agagcggcac
    28681 caacatacct gccagctacg acctcagcgc tgacatcatt aattcgtcgg tggccgttcc
    28741 cgcaagtctg gtgagctcaa tccagtttcg tgagaacaac gcgacaataa caagcaagaa
    28801 aatcgatatg ggcaagagtg agatccgcga catcactgtc aggattccgc aaatcccggt
    28861 gacatttatt gctggaacaa gttttacgtt gaaactgact gcgacgtcag gaagcgtgac
    28921 cgggaccgat cagcgctcaa ttacggttgg aacggcactc ccggtacctg atccaaagat
    28981 tgaagtcatt cagctccagc cacagatctt caacgtccag accggaaaca acgaagtgga
    29041 tccggccaac ggcagacttg agggctccac gatcaaactc aagaccggcc ggcaaatggt
    29101 aatcggattc aacgttaagc tcactcagaa cggcgtttac caggtaacga ttcaaccgag
    29161 acagggcaca acattgaccg gatggggacc agcgctcgtt gatacgcaaa ctaccattac
    29221 agtgcctaca aacaacgatc agaccacgcg gttgttgcag tttggagtca ctacctcgac
    29281 cgtccagaca cccagtccga caggaaccgt gatctttaag ataaaacgag atacagcgac
    29341 ggtagaattc aacaaagaat ttggcgtgga gcttttgccg taacacattg cttcgaagca
    29401 acacattagc aggcccgtca gacgggctga taaggagaca gccatgacag tatctattcc
    29461 cagcgccggt ggcgtacctg gcgcaagtgg tccgcccgac tggctaagtg gtggcgccgg
    29521 acaaaacttt agattcgacg acccaaggtg gcgcggagcc acaaagcttg atttcgccgc
    29581 tggcgccgca catggaatct ttttccgcgc aattcatgac aacagcggac aggaattcat
    29641 ttacatcacc ttgcgcgccg gatttgtgcc ggagatgaat acaggccgcg acttcgttta
    29701 catcggtctc caaagacacg gcacaactga cgcaatcgtg atcaagattc aagtgcacga
    29761 ttcggcgttt gcagatgcag gaccaccgtc ggggagcccg ccagttaacc cctttgttat
    29821 tcaagtaaac caatcaagcg ccggtggccc attctcgggc aacattgtgg gtaatccgaa
    29881 ttggatcaat gcgaacgcaa gagtgtggtt gcaaaacaat aacactgtac ccagtgatcc
    29941 caatttccgt tggggagttc agattcgaat tccggtgagt ggcgcgggcg gcattctaaa
    30001 taccaacggc ccgcaactgg gtagcgattt tgacatgtgg tacctgattc gcggctccgt
    30061 catgggaatg gcgaccatcc tgggcgagta ccgcaccgac ggaaataata cgtccgcatt
    30121 cgatctcggt aacagtaact atcctctgcc aacgggcatc tgggatgagt tcctgcttac
    30181 ttccggaccc gcgagtttcg gcggcgtcac catttttggc aacggacaga atgacgtcgt
    30241 tgttcagaat ggtctcggca ctggcacgac gatccaaaac ggcgcgctga atacgttcat
    30301 cgcacgacca cgcaactatc gagcgcctgg aaatgacatc gcgtcgggtg atattgccgc
    30361 cacgtttcgc attgcgaact ggggctcagt cgccaccgcc gctccgtggg attttgtgga
    30421 tcccgcggtt actactccag ttgcgagcac cgctgtgatt ccgacgctag cggctgccag
    30481 caatccgccg gctacgagtc cgattgcaca acccgtggtg atgaatctgc ccgcgggtaa
    30541 gagtactcac cagtgcgttc tgtgcacctt gtcgagcact ggtccagtca cgctttttct
    30601 gcaggacagt gtgttccgaa acatgaacta cgatcatgct tcgatccttg aacgagaagc
    30661 ggagatcgat accactggcc tgccaaaaga tgatgctccc gatcctcgag acgtgtatat
    30721 cgcgatcgaa aaggtgggca tggagcaaaa cacgcccggt gcagacgaag gaaccttcct
    30781 gagtaaccgc atcgtcagag caatgcgaag aggcgggcct attgcagaga agctgaagag
    30841 ggcgcaaggc cttctggatc aaataggccg aggggccgag ggccagaacc agcgcctgga
    30901 ggtccttgtc aacgtgttga ctgaggtggg actgaccgac gacgagttgg accaggtatt
    30961 tccaacgttc cgcgtgcacg tgtatcacga cacaggggag cgactaaaag aagacggtga
    31021 agagaaacct gtcttgcgtg ctcaatcatc gttcgggctc tacatgtatc acgaaggccc
    31081 ggtggaagga tggcaaacga gcatacaagg cgcgacacgt atttctgaca acgtttacca
    31141 gatgccagta ccaaaggacg gcagcgccaa gatcaaagtg atcgttcagg ccgtcgacaa
    31201 gggccaggac cgcataccgg aagatccaat catggcgcct agaggcgccg gtgaaattgg
    31261 aactggtcct ggagacagga aaggttgctg gctcgcgctt tggaagttgt ttggtttcaa
    31321 gtaaggcgtg gtagatgctt ggtacattca acgattccac catcctgagg ctgcgggtca
    31381 agggcagcga tcttgacccg ctcgccttgc gtggacgttt ggaaaatgtg ctgagcgacg
    31441 tttcattgca accgcgtctg ccgccgtcgg ccacgctctt cattcgcaaa ctcgacgatc
    31501 cgttgccggg atcgattcaa ctcgacagtc cacatgcgcg tgcgccacaa gcgtggcggc
    31561 aagcatttca gacgaggttc gatcggcttg tgtcgagtgc ggcgcgtccg gcgcacgggt
    31621 acgttgcaga cagtgcggag tcagtcgttt tttatgacta tgcggagttg ttggcgtgtt
    31681 tgacggcgga ttggtgcaac gggtcggcgc tgacgcgttg gtggtggcag gcgttcatca
    31741 agcgcgggga tctgtcgtgg ctgatcagga agttttggcg cgagcagatc gagtatgtgc
    31801 cggcggcgct cgagcatgtg gttaggaagg gagtactggt cgagtttgtg agccgattaa
    31861 gtgatgttga ggtacgcgag atcgtgcagc gcgtggtttc taccttcggg gtaacgcgga
    31921 ttttacggat tgaaaacgcg gatttttttc ctgttgatga ggcgcgtcaa atagaacatg
    31981 tcgcacagat cagaagtgaa atgccgtgga gtgatgtggt accggaaagc gatacgccaa
    32041 gtctgcgccc ggtgcagcag ttcttcgtcg ggatcgtgct gatggtccat cgcgcaccgt
    32101 cgcgcgtgcg gacgctgaac tttgcgcgcg aagtcgaaca ctggatccag gaacatttcg
    32161 tcgaagtgca acacgccgaa acgatcctca ccgaagaaat acccgaaccc gaactcttca
    32221 cgcccgctga acccccgacg ccgatccgcg taaatccgat tcacgcggat ccaattgctt
    32281 tcaaaccccc tccgccacca gcgggatccg taaaatccgt gttccgtaaa gcggagaaga
    32341 tcacgatcgc caatgcaact ttcagctacg aagaacccaa cccggttgta atcgagcaga
    32401 ctccggtatc agttattcag agcgaaacaa cactgccacc agaaccaatc gaagtaacaa
    32461 ccaccacccc tgaatcactc gatcccgcca caatcgaaac aaacttaggc ggcctcttct
    32521 acctgatcaa cctcgcgatc ttcctcgaaa tctataacga cttcaccagc cccgtagaac
    32581 agctccacga cgatctgagc atctgggact tcgtcacaat cgtcggtaaa gaactcgatc
    32641 cagaaaccga cgatccgatc tggcagttac tcagcaatct cgccgatcga gaagacgaat
    32701 taatcacacc aacctggtta accgatctac tcccgcacat acgcgcacga ttacacctcg
    32761 ccctcggcat cgacgacgat ctcgcggaaa tcctcctgca tcacgaagcc aaaataaccg
    32821 tcagcccgac acatctcgac gtcttcttct ccctctccga tcacccgatc gaaatacgcc
    32881 tctcaggtct tgaccgcaat cccggctggg ttcccgccgc cggccgattc atcgcttttc
    32941 actatgacta aaaagatgcg aaaaaatcta ttcgacggtt tgcagccgac tccggccgag
    33001 cacttcaagc tgtactttta tggagccgtc cttagcctct tccatcacgt ggtccaggtg
    33061 tacggctcga gtgaatcggc cttcaaagaa ttcccttttc tcatcggcta caacaacgag
    33121 ctcgccgacg gcggcctctc cggagtccac tcgactgaag ccgtttcgtg gtggctcgaa
    33181 tcgctgcaag catgggaaga aaaagcaacc ggtcacctcc cgttgcgggc actacgcaag
    33241 tccgcacagt tggaccactc cgcgctgata ctgctcacga ctgtcggttt gatcgaagag
    33301 gatctgcgtt tcggtctggt gtttgaaacc atgcagggct cgcccggcca gcatcgtgcg
    33361 acgccgagtt tgctcaacac gtggggacgc gatgaagcac actggcgcga cgcgcgcacc
    33421 aacctgcgac agatgcgcga gctcggactc gtgcgcgtcg tgaatccgga gtcgccgcgg
    33481 cttgaatggg cgctggaagt gccgagtgcg ttgtgggatg cgttgcgcgg cgagcaaaac
    33541 gagattcccg gatctggtat gcgctatcgc agtccggctg agcttgcgcg gatggaggat
    33601 ctgattctgc caccggacgt gcatgaaaaa gtgcgcgcga ttcccgggtt gttccgggcg
    33661 ggcgaagcac agacgttgat cgtacgcggc gcttatcgaa acggacggcg cacgatcttc
    33721 agcgcgattg cacgcgaact caatctcggg ctgctcgaga tcaaaggcat cattggacca
    33781 catgacgaac gattccgcgt cgctggacca ctcgccacgg cgctcaacgc actgcccgtg
    33841 atcgtcatgg atctcgagcc ccgtgaaagc atcgagattc cgcgtttgtc cgcgtgcgac
    33901 tcacccggcg gcatcgtcat cggcaaacaa ggtggtgtta gtggcgaggg agccgaacgt
    33961 gcgctcgtga tcactctcga catgccggac cccggcatgc gtaaacaaca ctggcagcgc
    34021 gcgtgtcacg aaagcaaact ccgcgatcct gagactgtca gcgagcgctt tcgtatcacg
    34081 tcgggaaaca ttcatcgcgt cgcgcgtctg gctgagtctt acgccgcgct cgcaaaacgc
    34141 accgagatca cgctcgttga cgcacaacgc gcaacgcgtt cgttgaatcg ccagattctc
    34201 gacgcactcg cgattcgcct ttccgcatca ggcgactgga gtcagctcgc tgttggtggt
    34261 gagacgcggc gcgagctgga aagcctcgtc agtcgttgtc gtcatcgcga gcgcctgcaa
    34321 tcgcaggctg atgcaggtgt acgtgcgctc ttcagtggtc caagcggcac cggcaagact
    34381 ctcgctgcgc gactgctcgc ttccactctg caaatggatg tttaccgtct cgacctttca
    34441 agcgtcgtga ataaatacat cggtgagact gagaagaatc taaacctgat cctctcccgc
    34501 gctgaagagc tcgacgtcat tcttctgctc gacgagggcg acgcgctgtt gacgcaacgc
    34561 accggcgttc aaagctcaaa cgatcgttat gcaaatcttg agacaaactt cttgttgcaa
    34621 cgactggaat cgttcgaagg cattctcgtc atcacgacga acgcgagcga gcggatcgac
    34681 aacgcgtttc agcgccgcat ggatgtggtg atttcgtttt cacctccttc gccgccggaa
    34741 cgctgggcca tctggcagct ccacttacca tccgatcatg cagtggatta ctccctgctc
    34801 gaagaagtcg caggacgttg cgtgctctca ggcggccaaa tcaagaacgc cgcactgcac
    34861 gcgtcgctgc tcgcactcga agacggcggc atcgtcacat ccgcaatgat cggcgacgcg
    34921 atcgagcgcg aatatcgaaa ggcgggcggc gtttgccccc tgcgccggaa ctgaaaatcc
    34981 atccatagat tacacagatt aatctgcgca atctgcgtaa tctgtggacc aagttgaggc
    35041 atttatattt cagcaaacat ccgcacaacg aaaccagcac cgagcgcgcc gcctccggcg
    35101 cggcaagcga ctcccgcgcg cacgtcgacg cccgccacgt ctcaagcccc accagccgtg
    35161 cggctccctg gtgtgctcgg caaaggtttg tttcgtactc acgacggcaa aatcatcgag
    35221 ctgccgaaag acatcaccgc cgaagaagcc gcgcggctcg aagctgaagc actcgccgca
    35281 cagaaaaaga tcggcaaagg accaccgccg caacccgtcc ccgacgtaaa gaagctcgac
    35341 aaaaaagaag acaaaaaaac gcagctcaaa gccctgccga agaaaaaacc cggcaaaggt
    35401 aaaggcaaac gcgcgcgtgg cggcggtgct gcagcgatga tgaaagctgc cggcacgagt
    35461 aaagtcgccc agtaccttgc ctcgaaggct gcgcccgtcg tcaacaaggg cttcgcgaaa
    35521 ttagatcaac tgcgaacaaa ccagcagacg cacgacagcg ctgacgagaa acgggcgcag
    35581 tcggaacaag cacaagtaat ccctgacagc gaggggcagt cgaagagcaa caccggccag
    35641 gtcaacgtcg tcagcgatcg tccggcgccg aaagtcgatc cgaacaaagg caaacagacg
    35701 cttcaggaaa acctgcgaga gaacgttccg aagtcgatcg aagacgtcga caacttcaag
    35761 cgcgacaaga aagcacaaca caccggcgcc gacgtgttga aggtggtcca aacggacaag
    35821 agcgcggtgg tctcgacgtt tcaggatatg ggcaccacac cgccgcccgc gccgcgtgag
    35881 tttgaacccg aaccgctgcc gccacctgag gccgcgcccg gaaccgccgc cctcacgctc
    35941 ggcaaagacg gcatcgcgcc gctgcaaaaa gagcacaccg atctctcgaa cttcaccaaa
    36001 gaagccgatg gcaagctgaa agaagaaggc gtcacccaag agcagctcga catggtcgac
    36061 agcggtgatc tcgccactgc gaataaagaa aagaagggga tggagaagtc ggcgataacc
    36121 gagccgctcg ccgtgcagca gtttgcgcag cagcaaaaca agaaagtcga gaacgatctc
    36181 aaacaggaag agtcgaagaa gcgctcggac atgaacgcca agcgcaaagg gaatctcggc
    36241 gttaccggtc agaaacagaa gaacacgaag agctcgctcg aaaagaagcg cgaagaagtc
    36301 gccacgaaga tcaacggcat ctacaccgcc gcgcaggaca aagttaagaa gaagctcgcc
    36361 gatctcgaaa cggcagcgat gaaacgtttc gacgacggca acaacaaagc gaccaaagat
    36421 ttcgaagaca ccgtcaaccg cgagctcgac gctttcaaag cagatcgata ctcgggctgg
    36481 tttggttggg ccagaaaagc gaaagattgg ctgctcggca tggacgattt gccgggcgtg
    36541 aaagccatct ttgatcgcaa tcgcgagaag tttgtcgcca ccatcaacaa gctcgtcgaa
    36601 gacatcaccg ctgacaacaa gaaagtgatc caggagtgca aggacgagct aacaaaagcg
    36661 cgcactgaga tcaaagacta cgtcgacaag cttggtccaa gtctaaaaga catcggcaag
    36721 aaagccgccg aagagatgaa ctcgaagctc gacgagatgg acaagttcat cgcgaagaaa
    36781 gaagaggatc tacaaaacca gctcaaagac aagcagaccg ccgcgatcaa agccatcgac
    36841 gagaagatcg agaagatgaa agaggcgatg gcgggcgcgc tcgcgaaact cggcaagctg
    36901 ctgctatggg cggcgaagaa gttcttcacg tgggcgctcg agaagtttgg gttctcgctg
    36961 ggagacattg agaacatcat caacaaaggc gccgcggtgc tgaaagcaat cttcacgaag
    37021 cctattcagt ttgtgaagaa cctgatgaac gccgccatca ccggtttcaa aaacttcggc
    37081 aaaaattttc tcaaacacct gcaggatgcc gttttcgatt ggctcacggg atcgatcgaa
    37141 ggattccaac tgccgacaac ctgggacttc aaaggcatca tcagcgtcgc gcttcagcta
    37201 atcgggatct cttacgcaaa cattcgccgt cacatggtgt cagtgatgac tgaacccgtt
    37261 gtcgtcggtc ttgaaaagag tttcacgctc gtgaagacgc tgatcaccga aggcccgctc
    37321 gccgcgtggg aacagttgaa ggagatggcg actgacatca aggacgcgtt cattcaggca
    37381 gtcaaagaat tcatcgctca gaagattatc gagcaggcaa ttcaatggat cgtcggcatc
    37441 ttcgttcccg gcgccggcat tatcaaagcc atcatcggca tttacgacac cgtcgtgttc
    37501 ttcatcaaga aagcgaaaga catcatcaag atggtcggta actttctggg atcggttagc
    37561 gagattgccg cgggcaacat cggcgctgcg gcccaggcca tggaggatgg tctcgcgcgc
    37621 ggtcttttgc tggtggtcaa ttttctcgcg gcgctgcttc gtctcggtgg cgtgaccgcg
    37681 aagattcgcg ccgtgctgca gaagatacgt gcgaaggtcg acgacgtgtt gttgaaagtc
    37741 gcgaagtgga tcgcgacaaa agcgaaggcg atcatcaaca aggttgtggg cgccgtcaaa
    37801 ggtgcggttg gatcagtgac ccagtggtgg aagcgccgca aaaagttcaa agccgacgac
    37861 ggccaaacac actcgctcgc gtttcatggc gagggccgcg ccgcacgtat gtgggtcgaa
    37921 agcgatccga cgccgtttga aacgttcatc aaggatgtga aagctgatgt gagtccgcag
    37981 aaggaagcaa aagcgcgggc cctcatcacg gccaaagaga tggacgagct acgaaataaa
    38041 gcttccgtca ctgatggcac gctgagcgat ccggacaccg tgaagttgca ggcgttgtgg
    38101 gacaagctcg cggacgacgt ggctattctc tttgccggcg gagccccgaa agagccgaaa
    38161 tatggtgtac caacaagccc ggcgggttgg ggctcgggaa tgttgatctt aaatctgaag
    38221 aacccgccaa aaggttcccg gccaaccgtc tcgaatccgc acttcattga cctcaacatt
    38281 cgacgttacg gaaatgtcac ttcgcccagc acggagagtt attacattct cggtcacctg
    38341 ctaaacgaga atttgaaggg tcctggaaat atatgggaga acattactcc gctttctcga
    38401 aaaggaaacg cgcaacatgt gacgcaagtc gaaacagacg ttaaagccgc tgcaacgtcg
    38461 aagattgtcc gctacgaagt gcaggcgcgt tacggtcgag cagccagcgc gctgctggcc
    38521 cagatcccgg ctaacactac ggatgctgca ctactgaata agcgcaaggt gttgctcgca
    38581 gagcaatacg ttcccagtac tttgctgtgc cgggcttaca ccgtcgacgc cagtgggaaa
    38641 ccggtcggtg ggttcaacct aacgaaagaa gttctgaaca aggtcgacga tagctctctt
    38701 gacgattaca acgtgccggg tcaggcagtg cagcggctca agaagctggc cattaacgat
    38761 gcgatcaaga aggcgcacgc gggtgaaaag aaatatgaag cggcgttgat gcaattaccc
    38821 ggcattggac caacgcggat cgtagaactg atcaatggtg gcaagccgtg ggggaaatgg
    38881 gaggactttt ggaaaggcgt taacggtgtg actaaggaaa acacggacga ctggcgtaca
    38941 aacggcattg ccggcggctc agtggacgtg atcctcaacg gtacctgtga atgggtgtga
    39001 cgacggctac aactaaagcg catggtccgg ttcaggcgca aaatgtacct tcgcgtgtgg
    39061 ctgcgccaaa gacgaagaat gtctcccgca gcggcgtaac ggcgcaacgg aagacacacg
    39121 ctactcttcg cgcctttgcg ccgctgcggg agaatccgtc gacacatgta agcctcacga
    39181 tcagcaaaaa gcagccaaaa aacgctttaa ttcagacggt ccagccgcct gctgacgtag
    39241 cgccgccaac gatgacgctc ggtcgcccgg ccagtcaggt tgttaaagat caaactctca
    39301 agaaactcga atcaaccacg atcacgagca cgggtgcgcc gttgccggtt gccgtgaaga
    39361 gccctttgac gcatagcttc tctgtcgatc tcacgccgat tcgcgtccac accgatgcgc
    39421 gcgcgcaact caccgtcaga agtttcaaca cgcgcgcgtt tgcttacggg caccacatct
    39481 ttcttggacc aggcgagtcg ccgagcgact tgcgactgat ggcgcacgaa gtggcgcacg
    39541 tggtccaaca aagtcgcggc gctgtaatac aaaacttcac taccggcaag ggcgatgcgc
    39601 tcgaacagga agccgaacgc gcctcaggag ctgccgtgcg cggcgagaag ttcaaggtgc
    39661 aacagcgcac ctcgccacga ccgcaaggtt tactcggtat cgatctcggc atacccaatc
    39721 cgctcgactg gctcgccgga aaagccaaca tgattccggg cttccgcatg ttgacgatca
    39781 tcctcggcgt gaatccgatc aacatgagcc cggtcgagcg cagtgccgcc aacatcctgc
    39841 gcgcgctgat cgagatcatg cccggcggcg gattgatcac acaggcactc gacaataacg
    39901 gcgtcttcga gaaagccggc gcattcgtcg aagaacagat caacgcgctc ggcctcactg
    39961 gttccatgat caagtcggct gttactgaat ttatcagcag cctgagtctg ccgggcgatc
    40021 tgttgaatcc gggcgcggcc tgggcacgcg ccgaacgaat cttcacagcg ccgatcgatc
    40081 agattaagtc gacggccgtc aatctcgtca ccggcatcat cgagatcgtt aaagacgcga
    40141 tcctcaaacc catcgcgaag cttgctgaag gcacggaagg ttacaacctg ctgaagggcg
    40201 tgctcggcaa ggatccgatc acgggcgacc cggtcgatcg cagcgccgaa aatttacttg
    40261 gaccactcct caagatgatc ggtctcggtg atgtttggca gaagatgcag gagtcgaagg
    40321 cgattccgcg cgcgtgggcc tggttccaga ccacgctttc gcaactcatc ggtttcgtca
    40381 gcgagattcc gacgctgttc ctgtcggcgt tcaagtcgct cacgctcgaa gacatcatcc
    40441 tcgttccgaa agccttcgcg aaactcgcag ccgtgttcgg cggcttcatg agcaagtttg
    40501 tgagctgggg cgtcgacgcg atgttcaagc tgctcgagat cgtcttcgac gtcgtgagcc
    40561 ccggcgcgct cggttacgtg aagcagaccg cctcggcaat gaagagcatc ttccagaacc
    40621 cgttgccgtt cgtcggaaat ctggtgaagg cagccaagct cggcttcatg aacttcggcg
    40681 gaaacttcct cacgcatttg aagaaaggac tgatcgactg gctcacggga tcgctgcagg
    40741 gcgtttacat tccgaaggct ttgacgctgc ctgagtttgg caagctcgcg ttgagcgtac
    40801 tcggtatcac gtgggcgcag attcgcggga agatcgtgaa ggcgctcgga ccgaacggcg
    40861 agatcatcat gcagggtctc gaggccgcct tcgacatcat caaggcactc gttaccggtg
    40921 gtccagcggc agcctgggaa gttatcaaag acaagctcac gggcctgaaa gacacgatcg
    40981 tcagcggcat catctcgttc gttgtcgaag cggtggtgac caaagcgata ccgaagctga
    41041 tcgcgatgtt cattccgggc gcgggcttta tctcggcgat catctcgatc tacgacacgg
    41101 tcatggtctt cgttaataag atctcgaaga tcattcaggt cgtcactgcg ttcatcaact
    41161 cgatcgtcgc gattgcgtcg ggcaacatcg gtgctgctgc cgccaaggtt gaaagtattc
    41221 tgggcgggtt gctgtcactc gcaatcagtt tcctcgcggg ctttgtgggt ctgggcaaag
    41281 tcactgacaa gatcatgggc gtggtgaaca aggtccgcgg cattgtcgat aaagcgctcg
    41341 acactgcgat caacttcatc atcggcaaag cgaaggcact ctttgccagc ttgttcggca
    41401 agaaagataa aacgccggat tcgcgaactg acgcccaaaa gatggctgac atgaaagcag
    41461 ccgtctccga aggtctgacg atcgtttctg acaagagctc ttccaagaag gcgaaagaca
    41521 agaacctgaa gaagctcaag agtaaataca agctcacgaa cattgaattg atgtccgatt
    41581 cgaaggcgaa ggaaacggag acagttcata ttcatgccac gatcaacccg acatacgact
    41641 caaacgggat catcatcgat gcatttccgc ctgcaccagt cgtaaaaatc gtggcgatcg
    41701 tcggcgcccc aattccgcgg caaggcatgg aggaagtgat cgaggagagg gccggatacg
    41761 aacgcgctca cttgcttggt ccaattctcg gacacgacct gccgcaggga gttatgtatg
    41821 cgccggtgga agtcaaccaa aagttacaga agagcggcat cgaaggtctc atcaaggcga
    41881 tgtacaggca acgtttcccg ggcgcggagt tcagagtcac gctgacggca acgcctcacg
    41941 cgggcggggg gaatatgctg gccagtgcga attatgtgtt gcacgggaaa ctcccgtccg
    42001 actcggattg gacgttcatc ttcgattaca cgatcaaggt cgggctcgga gcatcaccgt
    42061 caattgtggc taaggcggga gagcctcccg atatggaggc ggtcaatcgc ttctcgaccg
    42121 gtgtggcctc actcttggcc agaagggaaa ggcgctaaca actatgtatc tcgactcagt
    42181 aaaactattg caaatgcagg tcggctacgg cgacctcggg atgtatggaa gcctcggtta
    42241 tgaaggccgg caggtcacgg tgcgcaagca gcactaccaa catgccttca gcacacatcc
    42301 gccggcgcga ttgcattttc atgtcgacag gcggtttgcg agtttcgcgt gtcaggtcgc
    42361 gctgaatgac gacgtggctg cgggtttatc gcacgcggat ttttccgtcg ttgctgatgg
    42421 gcgtgaagtc gcgaacgagc catacgtgca agccggtgag ccgccgcgtc cactcgtcgc
    42481 agacatcagc ggcgctgagt atctcgagct ggtggtgcgt acgagccgtt gggaaaactc
    42541 gcacgccgtt tggctcgatc cgcaggttga cgaatcgccc gcgccggtca cgcatctctt
    42601 cgacagtttg agtcgcgcgg agatgatcat tcccgagcgt ttgccacgta ctgagcgctg
    42661 catcgccacc gttgtctcgc ccggattcga acatctgctc gacgacatgc tcggctcgct
    42721 ttacgcgaac ggttgctgca acgacgcgct cattgtcgtc ttcgtgctca atggcaacaa
    42781 ttcagttgaa cgcgtggtcg cgaagtacaa agcgaccgcg gtgcgttgcc agtcgcgcgc
    42841 gggtttgaat ccgatgagca aggcggtgat gtactcgacc gcgcgcgtcg tcgatgcgaa
    42901 gcattacctg tgtctcgacg ctgacatggt ggtgcttggc gatctgcgcc cgatcttcgc
    42961 gacgctcgaa gcgcttcctg aaggacgcat actctgttgc cgcgaaggga atagtcataa
    43021 cgccaatcat cttggctctg cgatcggatg gatctacggc ggcgggcagg ctgatttgtc
    43081 gaagctcggc gtcacgccgg aagagcaagc gtattcactc gtggtgaacg atggcctctt
    43141 cgccggcgac cagactgcgt tgcgcgcgct cgacggtgcg atacgcgcga tgccgcaggc
    43201 gtgtgcgtgg gttgatggac acagcaaggt gaagtggcgc aatcagttca tcttcaatct
    43261 cgcgctcgcg cgtttgcagt gcggcgtcga gctcgatccg ctgtacaacg tgcagcttca
    43321 cgtgcaggac gtgaatttct tcgagcaggg atcgcgcttg agggcgacgt ggcgcggtcg
    43381 cgagccgcgt gtgttgcact tcagtggggg cgcgaaacgg aagtatccgc agtggcaggg
    43441 gaagttttcg atcgccgatc ctttggcggg caagacgagt ggagacagtt atgccgtgtt
    43501 tgtcgaagcg ttgcgtgcgt ggctcggacg tcatggagta aacgcgctcg cgtggtcgtt
    43561 ttatggtttg acgagtggcg acagtgcctc ggtcaaagat ccgtcaacac tgccgctgct
    43621 cgcgacgttg cattatctga ttcgcgcgaa tggatgtgtg cgcgtgctcg aaacgggaac
    43681 ggcgcgcggc atctcggcgg cgtgtctggc ttccgcggtt gcgcatcgcg agggcggacg
    43741 cgtggtcacg ctcgatccgt ttctgcatgc gggccgcgaa gagctctggg ccatgttgcc
    43801 ggaaggttat caaagctgca tcgaggcgcg gcctgtcgat tcgattgcgg gcatgcagat
    43861 ggcgttggat gctgatgaag tttatgaagc tgtgttgttg gattcgttgc acgaggccga
    43921 acacgtttgg gcggagtttc aagttgctac cgagatcgtt tgtccgggcg gtttgatctt
    43981 gattcacgat gcgacttacg ccggtggcac agtgcccgcg acgttagcgc gcattgaagc
    44041 ggctggttac aacgtcgtgc gattgtgggc tgcggatggc ggcacggctg aagacgatca
    44101 tctgggtctc gctgttatcg agaatcgtcg acgacgagtg cccgtgattt tggagagtca
    44161 aaatgaattc gccgaacggg tttccgcctg acttcgagtc gagcgacatc gagatcatcc
    44221 gcgcggtcga gccgtacacg atgacgagca cggagcggat ccatgcgttg atccatgcag
    44281 tcagacatat cgtgcgcaat cgcatcgcgg gcgacatggt cgagtgtggc gtgtggaagg
    44341 gcggaagcgt gatggcgatg gcgttgacgc tgttgcagct cggcgaacgc gatcgatcgc
    44401 tttatctgtt tgacacgttc tcgggtatga cggcgccgag tgatgtagac gtggattacc
    44461 agggacagca ggcgcacgtg attctcgatg cggtgcgttg tgaggcgtcg cagcaggaag
    44521 ttgagaaggc agtgcttagt actggctacg atcgggagag aatccacttc gttccggggc
    44581 gcgttgaaga gacgatccct ttaagcgcgc cagcatcaat cgcattgcta cgactcgaca
    44641 ccgattggta cgagtcgacc caacacgagc ttctgcactt attcccgcga ttagctcgtg
    44701 gcggcgtgat catcatcgac gattacggcc actggcgcgg cgcccgccag gccgtcgacg
    44761 aatacatcgc cgaaaaccaa atcccgttac tacttcaccg catcgactac accggccgca
    44821 tcgcggtgaa gatatctgtg taatctgtgg atggctttaa ggaggaaagg tgggttatct
    44881 cgaagattcg ctgaacctga cgttgggcca gatcattccg ctcatgcagc agcgcatcat
    44941 gaacgagtcg acgtacttcg gcgtgaagac gtggaaatgc ccgatcgatt tctggatcta
    45001 ccaggaaatg atcttcgagc tcaaaccgga cgtgatcgtc gagatcggta atcactcggg
    45061 cggcagcacg ctcgcgctcg cacacatctg cgatctgctc gacaacggtc gcgtgatcgg
    45121 acttgacctc tcacacgccg ccgtccccca taaggtaaaa gaacaccagc gaatcaaact
    45181 gatcgaaggc gatgcctgtg cgaacttcga tcgcgtcgcc caactaatca aacccgaaga
    45241 tcacgtactc atcatcgaag acagctcaca cacgttcgaa aacactctcg ccgtcctcga
    45301 tgccttttca cggctgatca aacccggcgg ttacttcatc gtcgaggacg gcatctgcca
    45361 tcacggcctg aacggtggtc cgtcgcccgg tccatacgaa gccgcagaag ccttcgtcag
    45421 ccgcaacaac tgtttcgcga tcgacaggag ccgcgaagct tttctgatca catggaatcc
    45481 gaagggctac ctgcggagat
//
LOCUS       DASSFQ010000030         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1502734, whole genome shotgun sequence.
ACCESSION   DASSFQ010000030 DASSFQ010000000
VERSION     DASSFQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502734"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFH96_01070"
     CDS             complement(<1..596)
                     /locus_tag="VFH96_01070"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET6972579.1"
                     /translation="MSATFATEEVLIIETPERVPLHFALASIGNRFLACAIDHTIQAL
                     ALGLITIACVTLMNFSFIEQTISSAPKWVLALMLILLFLVLAGYFTFFEWIWSGQTPG
                     KRWLKLRVIREDGRPITFWEASVRNLLRALDMFPVGSYSIGLIAVFASTRDQRIGDMV
                     GGTVVVREREAEAPEFAKVFATPVSDPAMRRSFKPVDFT"
     gene            complement(631..1839)
                     /gene="tyrS"
                     /locus_tag="VFH96_01075"
     CDS             complement(631..1839)
                     /gene="tyrS"
                     /locus_tag="VFH96_01075"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET6972580.1"
                     /translation="MNIDEQLSYLGKGTVEVIRQAELRAKLEKSAATGKPLRVKLGAD
                     PTAPDIHLGHTVVIRKLRAFQELGHIAIFLIGDFTGMIGDPSGKSTTRPQLTREEINA
                     NAETYKQQIFKLLDPERTVIDFNSRWMDTLGSAGFLRLAGHVTVRQILERDDFQQRLE
                     ANRPLALHEVMYPLVMAYDSVALEADVELGGTDQKFNLLMGRNLQREYGQESQVAMIT
                     PLLEGTDGVQKMSKSLGNYIGINEPPQEIFGKIMSITDELMWRYYELCTDLSVGEIAA
                     LRNSANSSGRNPRDIKAALAKHIVADFYSEAEACKAEEEFNAMFRNKQAPEDIEERTL
                     SSGSWKLPKLLVELALVPSMAEARRVIEQGGVYVDGERRTQTDFEIDLKSDKSVLIQV
                     GKRKFIRVRG"
     gene            1888..2745
                     /gene="proC"
                     /locus_tag="VFH96_01080"
     CDS             1888..2745
                     /gene="proC"
                     /locus_tag="VFH96_01080"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET6972581.1"
                     /translation="MADTNPLSNCRFAFIGCGVMAEVIIARLLRKKLVGPEQIIGSHP
                     RQTRREEMHVKYGIEMLESNREAVTETQPEGPEDSSIIVLAVKPQRLHVVLEELKGAL
                     VKEQLVVSIVTGAKIETIAEQLLHASVVRTMPNTPAQIGEGMTAWTASSEVNEKQESQ
                     VRAMLDALGKNARVENERQIDMATALSATGPTYIYLMMEALIDAGVHMGFSRRVAEDL
                     VHQMMRGAVLFAIESHKHPAELRNMVTSPGGTSAEAIYQMEKGSLRTVLSKAVWAAFQ
                     RAESLGKTI"
     gene            complement(2767..3336)
                     /gene="purN"
                     /locus_tag="VFH96_01085"
     CDS             complement(2767..3336)
                     /gene="purN"
                     /locus_tag="VFH96_01085"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET6972582.1"
                     /translation="MKKRIAILISGRGSNMLALSDAVRDGVIPDAEIAIVISDKPGAR
                     GLELARERGIEAVAIERRGRNREEHEHEIISALRERHVDLVCLAGYMRLLSPCFVEAF
                     RGKILNIHPSLLPDFPGLDAQRQALEHGVQVSGCTVHFVDETLDGGPIIAQRTVPVIE
                     GDTVDSLSARILEQEHQLYPEAVALVLSK"
     gene            complement(3333..>4097)
                     /gene="purM"
                     /locus_tag="VFH96_01090"
     CDS             complement(3333..>4097)
                     /gene="purM"
                     /locus_tag="VFH96_01090"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET6972583.1"
                     /translation="LVNHCVNDILVQGARPLFFLDYIATGKLSPDVVASIVEGVTNGC
                     RENGCVLLGGETAEMPGFYQDGEYDIAGFIVGVVDRAKIIDGKTIAPGDVLLALPSAG
                     LHTNGYSLARKLFFEVAGYRPDTHLPELSMTAGDALLLPHVSYLKPLDGLLDKGVIKG
                     LAHITGGGLTDNIPRILPEGTAVRIERGSWPIPPLFELLRKLGNVSDAEMYRTFNMGV
                     GMVIVCAASDAEAIESQLIESYRIGKVVSGSREVLI"
BASE COUNT          944 a         1189 c         1112 g          852 t
ORIGIN      
        1 gtgaaatcaa caggcttgaa agatcgtcgc atcgccggat cgctcacggg cgtcgcaaac
       61 accttcgcga actcaggcgc ttcggcttca cgttcacgaa cgacgaccgt gccgccaacc
      121 atgtcgccga tgcgctgatc gcgcgtcgac gcaaagaccg cgattaagcc aatcgaatac
      181 gatcccaccg gaaacatatc gagcgcacga agcaaattcc gcacggacgc ttcccagaac
      241 gtgatcggcc gcccatcctc gcgaatcacg cgcaacttca accagcgctt gcccggcgtt
      301 tgcccactcc aaatccactc gaagaacgtg aagtaaccgg caaggaccag aaacagaaga
      361 atgagcatta acgcgagcac ccacttcggc gcgcttgata tcgtttgctc gatgaacgaa
      421 aaattcatga gggtgacgca ggcaatagtg atgagtccca gcgccagcgc ctgaatcgtg
      481 tggtcgatcg cacacgcaag gaaacgattg ccgatcgacg cgagcgcgaa gtgcagcggc
      541 acacgctccg gcgtctcgat gattaatact tcctcagtcg caaatgttgc tgacatttag
      601 taaatccgta aaaatccgcg tttattcgcg ttaccctctt actcgaataa acttacgctt
      661 accgacttga ataagcaccg atttatctga tttcagatcg atctcgaaat cggtctgcgt
      721 ccgtcgctca ccatcaacat aaacgccgcc ctgctcgatc acgcgacgcg cttcagccat
      781 cgacggcacc agcgcgagct cgaccaaaag cttcggcagc ttccacgatc ctgacgatag
      841 cgttcgctcc tcgatatctt ccggcgcctg cttgttacga aacatcgcat tgaactcttc
      901 ctcggccttg cacgcttcgg cttcggaata gaaatcagcc acgatgtgtt tcgcgagtgc
      961 ggctttgatg tcacgcggat tgcgtccact actgtttgca ctattacgca acgccgcgat
     1021 ctccccaacg gaaagatccg tgcacagctc ataataacgc cacatcagct cgtcagtgat
     1081 cgacatgatc ttcccgaaga tctcctgcgg cggctcgttg atgccgatgt aattccccaa
     1141 actcttcgac atcttctgca cgccgtcagt accttcgagc aacggtgtga tcatcgccac
     1201 ctgtgattcc tgtccatact cacgttgcag attgcggccc atcaacaagt tgaacttctg
     1261 atcggtgcca ccaagctcga cgtctgcttc gagcgcgacc gagtcgtagg ccattacgag
     1321 cgggtacata acttcgtgca gcgcgagcgg ccggttcgct tccagtcgtt gttgaaaatc
     1381 atcgcgttcg aggatctgtc gcacagtgac gtgcccggcg agacgcagaa aacctgccga
     1441 gccgagcgta tccatccagc gtgagttgaa atcgataacc gtcctttccg gatcgagcaa
     1501 cttgaagatc tgctgtttgt aagtttccgc gttcgcgttg atctcctcgc gcgtgagctg
     1561 cgggcgggtg gtgctcttgc ccgaaggatc gccgatcatg ccggtgaagt cgccgataag
     1621 aaagatcgcg atgtggccca actcctggaa cgcgcgcagc ttgcggataa cgaccgtgtg
     1681 gcccagatga atgtcgggtg cggtcggatc ggctccgagt ttcacacgca gaggcttgcc
     1741 ggtggcggcg gatttttcga gcttcgcgcg gagctcggcc tggcgaatga cctccaccgt
     1801 gcctttgccg agatatgaga gttgttcgtc aatattcatc gctggataga gtagctcaac
     1861 tattaaagtg tgcgctatcc ttgttttatg gctgatacaa atcctcttag caattgtcgt
     1921 ttcgcgttta tcgggtgcgg tgtgatggcc gaggtaatta ttgccaggct actccgcaaa
     1981 aagctggttg gtcccgagca gattattggt agtcacccgc ggcagacgcg gcgtgaagag
     2041 atgcacgtga agtatggcat cgagatgctc gagagtaatc gcgaggctgt cactgaaacg
     2101 caacccgaag gaccagagga cagctcgatc atcgtgctcg ccgtaaagcc gcaacgtttg
     2161 catgttgtgc tcgaggagtt gaaaggcgcg ctcgtgaagg agcagctcgt ggtctcgatc
     2221 gtcacgggcg cgaagattga gacgattgcc gagcaactcc tgcatgcgtc ggtcgtccgc
     2281 acgatgccaa acacacccgc gcagattggc gaaggcatga cggcgtggac agcgagcagc
     2341 gaagtgaacg agaagcagga gagccaggtg cgcgcgatgc tcgatgcgct cggcaagaat
     2401 gcacgggttg agaatgagcg acagatcgac atggcgacgg cattgagcgc gactggaccg
     2461 acgtatatct atttaatgat ggaagcgctg atcgacgcag gcgtgcacat gggcttttcg
     2521 agacgcgtgg cggaggatct ggtgcatcag atgatgcgcg gagcagtgtt gtttgcgatc
     2581 gagtcgcaca agcatccggc ggagctgcga aacatggtta cgtcaccggg cggcacgtcg
     2641 gctgaagcga tttatcagat ggagaagggt agcttgcgga cggtattgtc gaaagctgtc
     2701 tgggcggcgt tccagcgcgc cgaatcgctc ggaaaaacta tataagtcct attcgtccta
     2761 taggacctat ttagaaagaa cgagggccac tgcttcagga taaagctggt gctcctgctc
     2821 gaggatccgc gcggacaaac tatccaccgt atcgccctca atcaccggca ccgtacgctg
     2881 cgcgatgatt ggaccaccat cgagcgtctc gtcaacaaag tgcaccgtac acccgctcac
     2941 ctgcacacca tgttcgagcg cctgtcgctg cgcatcgagc ccgggaaagt caggcaagag
     3001 actcggatga atattcagta tctttcctcg aaacgcctca acaaaacacg gcgacaacaa
     3061 ccgcatatat ccagccaggc aaaccagatc cacgtggcgc tcgcgcagag ctgagatgat
     3121 ctcatgctcg tgctcttcac gattacgccc gcgacgctcg atcgcaaccg cttcaattcc
     3181 acgctctcgc gcgagctcca acccgcgcgc acccggctta tcactgatca caatcgcgat
     3241 ctcagcgtcc ggtatgacac cgtcacgaac cgcatcactg agcgccagca tgtttgatcc
     3301 gcgaccggag atcaggatcg caattctctt tttcagatta gtacctcgcg actgccgcta
     3361 acgactttgc cgatccggta agactcgatt agttgtgact caatcgcctc cgcgtcgctc
     3421 gcggcgcaaa cgatcaccat gcccacgccc atattgaacg tgcgatacat ctccgcatcg
     3481 gaaacgttac caagcttacg caacagctcg aacaacggcg gtatgggcca cgagccgcgc
     3541 tcgatccgca cggcagtacc ttccggaagg atgcgtggaa tgttgtcggt gagtccgccg
     3601 cccgtaatgt gggccagtcc tttgatcaca cctttgtcga gcaagccgtc gagtggtttc
     3661 aggtaactga cgtgtggcaa cagcaatgcg tcaccggcag tcatactcaa ctcaggcaaa
     3721 tgtgtatcgg gtcgataacc cgctacttcg aaaaagagtt tacgggcgag cgagtagccg
     3781 ttcgtgtgta gtccagccga aggcaatgcc agcaaaacgt cgccgggtgc gatcgtctta
     3841 ccgtcaataa tcttcgcgcg atcgaccacg ccgacgatga agccggcaat gtcgtactcg
     3901 ccatcctggt agaaacccgg catctccgct gtctcgccgc cgagcaacac acaaccattt
     3961 tcacgacagc cattcgtcac gccttcgacg atgctcgcga caacgtcagg cgataacttt
     4021 ccggtcgcga tgtagtcgag aaagaagagg ggtcgcgcgc cctggaccag aatatcgttg
     4081 acgcagtgat tgacgag
//
LOCUS       DASSFQ010000031         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_313104, whole genome shotgun sequence.
ACCESSION   DASSFQ010000031 DASSFQ010000000
VERSION     DASSFQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313104"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /gene="argS"
                     /locus_tag="VFH96_01095"
     CDS             complement(<1..1788)
                     /gene="argS"
                     /locus_tag="VFH96_01095"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET6972584.1"
                     /translation="MTLNELQQKLRDRIGVAARETFGVELQQVNAEMPPRAELGDLAF
                     PVSFELAKLVKQATGEKQNPRNIAEKLKTQLEDIDEVARVEIAGAGYINVFYDRAKLL
                     GMFAGPTQTERALSDRPKKMVEHTSINPNKAAHIGHVRNAVLGDTFVNILKAAGDRIE
                     VQNYIDNTGVQVADVVVGFLHLEQMDLDQIKALDASLNKDYTFDYYCWDLYTKVGLFY
                     RDGSAAGAMNPEKVKLRNEVLHALEEGGTNPIAQLADYVATRNVECILDTMERLGIRY
                     DLLARESDILHLHFWERAFELMKNTGVIRFEPEGRHAGCWVMPFESHTGTDEHESDKI
                     IVRSNGTVTYTGKDIAYQLWKLGRLGLDFHYRVFRNYEDGHVLWSTQTEPEQSGIDRP
                     HFGGGVTVYNVIDSRQSYPQEIVARGVAAVVPEIGEDASIHFSYEMVALSPAACDELG
                     IELSDEDRARPYIEMSGRKGLGVKADDLIIQLEAGALTEVEKRNPELSENDKKATAHE
                     VAVAALRYFLLKFTRNTVIAFDFKEALSFEGETGPYCQYAAVRTNSIFRKLPDETVAA
                     SDTLIKQIAGDSALQTRVSEVLSGGGGTEI"
     gene            1941..2663
                     /locus_tag="VFH96_01100"
     CDS             1941..2663
                     /locus_tag="VFH96_01100"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET6972585.1"
                     /translation="MQISSELRRRFAEARRVVVLTGAGVSAESGVPTFRGGGNTTVWK
                     GIPFDVISSAEMLERNLPAVWEWFDYRRDFMESLLPNAAHYEIARWQEQFPEFTVVTQ
                     NIDGLHQKAGSRNVVELHGNIWRARCRTCRAVHDLSSTAKRPDGCLECGDWLRPDVVL
                     FGEFLPPGAFELAAERAASCDLCIVIGTSGLVYPAAGLPEIARSAGAFLCEVNPEPTP
                     LSRLCDETLIGKAGEVLPLLRI"
     gene            2660..>3621
                     /gene="larC"
                     /locus_tag="VFH96_01105"
     CDS             2660..>3621
                     /gene="larC"
                     /locus_tag="VFH96_01105"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET6972586.1"
                     /translation="MKTLYFDCFAGASGDMILGAMVAAGVDPNYLRAQLSSLPVTGFE
                     INFETVNRSGLSATYARVETAHEHKHRHLSDIKQIIEASGLSAPVKQRAIQIFTRLAE
                     AEARVHNEPVDHVHFHEVGALDAIVDVVGAAVCFDALKIDQFICSPLHVGSGMVKMAH
                     GQFPIPPPAVAELLKGVPFYATDIKGELLTPTGAAIITTVCSSYGPIPPLTTDITGYG
                     AGTREYQDFPNVLRVMIGETETNTATDDRLWMLETNLDDSSPQIIGHVMDRVLELGAL
                     DCFFTPVQMKKNRPGVLLSVLCRREEKEAVMKLLFMETTTLGVR"
BASE COUNT          740 a         1016 c         1034 g          831 t
ORIGIN      
        1 gatttccgtt ccgcctccgc cggacaacac ctctgacact cgcgtctgca aggcgctgtc
       61 gccggctatt tgttttatta acgtatctga cgcggcgaca gtttcgtctg gcagcttgcg
      121 gaagatcgag ttcgtgcgca cagccgcgta ctggcaataa gggccagtct caccttcgaa
      181 cgaaagcgcc tctttgaaat caaaagcaat cactgtgttg cgcgtgaatt tgagcaggaa
      241 atagcgcagc gcggcgacag caacctcgtg cgccgtagct ttcttgtcgt tttcggaaag
      301 ctccggattt cgtttctcaa cttccgtgag cgcgcctgct tcgagttgaa taattaagtc
      361 gtctgcttta acgccgagac ctttgcgccc gctcatctcg atataaggcc gcgcgcggtc
      421 ctcgtcgctc aactcgatac cgagctcgtc acaggcagca ggcgagagcg ccaccatttc
      481 gtagctgaag tgaatgcttg cgtcctcgcc gatttccggc actactgccg cgacgccacg
      541 agcgacgatc tcctgcggat acgactgccg cgaatcgatc acgttgtaaa cggtcacgcc
      601 gccgccgaag tgcggccgat cgattccgga ttgttccggc tcggtttgag tggaccagag
      661 aacgtgaccg tcttcgtagt ttcgaaaaac tcgataatga aaatcgagcc cgagcctgcc
      721 gagtttccag agttgatagg cgatgtcttt gccggtgtaa gtgacggtgc catttgaacg
      781 cacgatgatc ttgtctgact cgtgttcgtc agtgccagtg tgcgactcaa aaggcatgac
      841 ccagcaaccg gcgtgacggc cttcgggctc gaaacggatc acgcctgtat ttttcattag
      901 ctcgaaggcg cgctcccaga agtgcaggtg caaaatgtct gactcgcgtg cgagcaggtc
      961 gtatcgaata ccgagtcgct ccattgtgtc gagaatgcat tccacgttac gcgtagcgac
     1021 gtagtcggca agttgggcga ttggatttgt gccaccttcc tcgagtgcgt gcagcacttc
     1081 gttgcgaagc ttgaccttct cggggttcat cgcgccggcg gcgctgccat cgcgatagaa
     1141 gagaccgacc ttggtgtaca gatcccaaca gtagtaatcg aaggtgtaat ccttattgag
     1201 cgaagcgtcg agagctttga tctggtcgag atccatctgc tccaggtgca aaaagccgac
     1261 gactacatcg gcgacttgca cgcccgtgtt gtcgatgtag ttttgcacct cgatccgatc
     1321 gccggcagct ttcaggatgt ttacaaatgt atcgccgagc acggcattgc gaacgtggcc
     1381 gatgtgtgcg gccttgttgg gattgatact cgtgtgctcg accatcttct tcggccggtc
     1441 cgacagcgct cgctctgttt gagttggacc agcgaacata ccgagcagct tcgcgcgatc
     1501 gtagaagacg ttgatgtagc cggcgcctgc gatctcgacg cgtgcgactt cgtcgatatc
     1561 ttcgagttga gtcttgagct tttccgcgat gttacgtggg ttttgctttt cgccggtggc
     1621 ctgtttgacg agtttcgcca gttcgaatga gacgggaaag gccagatcgc cgagctcggc
     1681 acgcggcggc atctcggcgt taacctgttg cagctcaaca ccgaacgtct cacgtgcggc
     1741 gacgccaatt cggtctcgca gcttctgttg cagttcattt agggtcatag gacaaaagcg
     1801 cagagtatag caaaattttg gagtgcggcg gcccggcgcc gctttggcct cgattaggcg
     1861 cgatcaataa caaagcggcg ccaggccgcc cgcactccat agaggcgtcc gtttggacga
     1921 tctgctacca tcgccgtgta atgcagattt cctcagaatt gcgacggcgc tttgccgaag
     1981 cgcggcgggt cgttgtgctt accggcgccg gtgtgtccgc ggagtcggga gtgccgacat
     2041 ttcgcggcgg cggcaatacg acggtctgga agggaattcc tttcgacgtt atctcgtccg
     2101 ccgagatgct ggaacgaaat cttccggcgg tttgggagtg gtttgattat cgccgcgact
     2161 tcatggagag tttgctacca aacgccgcgc attacgaaat cgcacgctgg caggaacagt
     2221 ttcccgagtt caccgtcgtc acgcagaata tcgacggtct gcatcagaaa gccggctcac
     2281 gtaacgtcgt cgagcttcac ggcaacatct ggcgagcgcg gtgcagaacc tgccgcgcgg
     2341 tccacgatct gtcatcaact gcgaaacgac ccgacggctg tttggaatgc ggcgactggt
     2401 tgcggcccga tgtggtccta ttcggcgaat tcctgccgcc cggagcattt gaactcgcag
     2461 ccgaacgcgc ggctagttgc gatctgtgta ttgtgatcgg cacgtctggt ctcgtgtatc
     2521 ctgctgcagg tttgccggag atagcgcgat ccgcgggcgc gtttctctgc gaagttaacc
     2581 ccgagccgac accattatcg cgactttgcg atgaaacatt gatcggaaag gcgggagagg
     2641 ttttgccatt gttgcgaata tgaagactct ttacttcgat tgttttgccg gcgcgagcgg
     2701 cgacatgatt ctcggtgcga tggttgccgc cggagttgat ccaaactatc tccgcgcgca
     2761 actgtcatcg ctacctgtca ctggctttga aatcaatttc gagaccgtca atcgatccgg
     2821 tctgagcgcg acctacgcac gtgtagaaac ggcacacgaa cacaagcatc gacacctgtc
     2881 ggatatcaaa cagatcatcg aagcctccgg tctatccgcg ccggtgaaac aacgcgccat
     2941 ccagatcttc acgcgcctag ccgaagccga agcgcgcgtg cacaacgaac ctgtcgatca
     3001 cgttcacttt cacgaggttg gcgcgctcga tgcgatcgtc gacgtggtcg gcgctgccgt
     3061 ctgcttcgac gctttgaaga tcgatcagtt tatttgttcg ccactgcacg taggcagcgg
     3121 gatggtgaag atggcccacg ggcagtttcc aattccgcca ccggcagttg ccgagttact
     3181 caaaggcgtc ccgttttatg cgacagacat caaaggtgaa ctgctgacac caacaggcgc
     3241 tgcgatcatt accacggttt gcagctcgta tggaccaatc ccgccattga cgactgacat
     3301 caccggctac ggcgccggca cgcgtgaata tcaggacttc ccaaacgtcc ttcgtgtgat
     3361 gatcggtgaa accgaaacca acaccgcaac cgacgatcgc ctatggatgc tcgaaacaaa
     3421 tctcgacgac tcgtcgccac agatcatcgg acacgtgatg gatcgcgtgc tcgagctcgg
     3481 cgcgctcgac tgtttcttca cgcccgtgca gatgaaaaag aatcgtcccg gcgttctgct
     3541 ttcagtgctc tgccgccgtg aagaaaagga agcagtgatg aagttgttgt ttatggagac
     3601 gacgacgctc ggtgtgcgca g
//
LOCUS       DASSFQ010000032        10846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_464481, whole genome shotgun sequence.
ACCESSION   DASSFQ010000032 DASSFQ010000000
VERSION     DASSFQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10846
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464481"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2190
                     /locus_tag="VFH96_01110"
     CDS             <1..2190
                     /locus_tag="VFH96_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6972587.1"
                     /translation="YLATNPASAVLRVDSADDDLVKAILQNPEVSDAEPRRTISGQLK
                     AGPMQWRNLVLFVVQDYGHIRVSRLVPESGAWPPANGEILIERDAFQVARANIGEAVT
                     VKTANGVEQPLVISGSVHDVGQAQARMENIVYGYINLATLVQLGEQPQLNQINILVAQ
                     NRFDEAHIRRVVADVEALIRSRGHEVLRVEIPRPGKHPHSDLTGILLLAMSSFGLFVL
                     VLSGILVINLLTAMMASQVRQIGVMKAIGGTRRAIAGIYFSQALFLGIAAVIVSIPLG
                     ILGSRALCRYMAYFLNFDINSFAVPFWVYLLVGLVGIAAPLLAAAYPVWRGTVTPVRV
                     ALSDFGLSQTNFGASAFDRALTRIGGRFSLIALAIRNSFRRRARLVLTLLTLTAGGLF
                     FLAALNVRSSMINTLDHMFGLRKFDLTVGFANAYEVEKIQKAVSNTSGVRRAEGWFVT
                     EGAHGDDRLTIVALPPDTHLQEFDIVEGRRLMPGDTDAIVINNALAGRDPKMRVGELV
                     TLRIGTDEKVWRVAGIAREAFNPTPVGYIPLTGMVNSLRLALDNTDPDSIGALKTELD
                     RNLEQQGIRARGSSTKSESRFGFDQHMLMIYVFLIITSAIIGGVGGLGLMTTMSLNVL
                     ERRREMGVMRALGATPRLIWLMIVAEGIVIGLLSWTIAALLAWPISKLVGNFLVQIMF
                     RSGLDFTFEPVGLVIWLAVSISLSALASFLPAWKASRTTVREALAYE"
     gene            complement(2211..3131)
                     /locus_tag="VFH96_01115"
     CDS             complement(2211..3131)
                     /locus_tag="VFH96_01115"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET6972588.1"
                     /translation="MKTPYHAALGNSLELDSLLAGSRALVEVELNELLPSSATAPEKI
                     HQAIRWSVFAGGKRFRPALLLAVGQAFDVPFEALIGTACAFEMVHTYSLIHDDLPAMD
                     NDDLRRGRPTCHVRFDEATAILAGDALQTLAFQTIADDDCINFETRIMVISELARAAA
                     TPHGMVAGQALDIEGEARQVSGTQLEEIHRLKTGALIVAAARCGAIIGKASDEELDAI
                     TSYGSHLGLLFQITDDFLDVTATAEDLGKTPGKDARSNKATYPALHGLEETRVAAERA
                     HDNAIASLNQINRPTTVLQQIAEFILHRTG"
     gene            complement(3128..3391)
                     /gene="xseB"
                     /locus_tag="VFH96_01120"
     CDS             complement(3128..3391)
                     /gene="xseB"
                     /locus_tag="VFH96_01120"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET6972589.1"
                     /translation="MENSKEQGRTFEASLEALEQIVRQLEDGDLPLEKSLELFEQGIR
                     LSRECQERLSQAERRIEILLRDNQGKPVAAAYKEPPAEATGEE"
     gene            complement(3411..4121)
                     /locus_tag="VFH96_01125"
     CDS             complement(3411..4121)
                     /locus_tag="VFH96_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972590.1"
                     /translation="MRQRIVINLDGPKGGVAQRAGGKRRRWPRVLAILALLIVIGVAG
                     LAIGGFFWWRRYQSTPNYAVTLLVDAAQRNDVGAFGKLINDDEVAKNITASVSQKAAA
                     RYGYALNSSIQQRIDGLMPTLLPQLKKTIHDELVKDLTVFSAEVKPKGFIGHLFVLPK
                     FVTIKSEGDIATVEARMLDRAIELKLKRDAEQWQVVEVKDDVLVQGIVDNVMKELPAI
                     GSVDPLLKKPQRKRQRRK"
     gene            complement(4138..5409)
                     /gene="xseA"
                     /locus_tag="VFH96_01130"
     CDS             complement(4138..5409)
                     /gene="xseA"
                     /locus_tag="VFH96_01130"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET6972591.1"
                     /translation="MNQPLFNALFDETERKPLTVSQLTNSIRIALEGRFAAVWVEGEI
                     SNFKDHSSGHWYFTLKDENAQLRAKCFRSTNTRIRFRPANGLKVRARGKLSVYAPRGE
                     YELVVDALDPVGAGALQIAFEQLRDRLQKEGLFAKELKRPLPVFPRRVGVVTSPTGAA
                     IRDILNVISRRTRTVHVLFSPAKVQGDAAGPDIARAIRFINLYHERALREGRPEDAVD
                     VLIVGRGGGSTEDLWAFNQEEVARAIRKSAIPVISAVGHETDYTIADFVADFRAATPS
                     AAAEIVAAREDQICSTLDHTGRQLVRLTKFQIVNLRARVQQQALSHAFDEVKSRLNNA
                     RARLDAASSACDGAIAEKLQEGRERLGLAAASLDALSPLGVLQRGYAIAQDASGKLVR
                     DAASVSEGDVVNVRLQKGKLRTRIERIEATD"
     gene            complement(5418..7103)
                     /locus_tag="VFH96_01135"
     CDS             complement(5418..7103)
                     /locus_tag="VFH96_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6972592.1"
                     /translation="MSFQRLAQLLFLITGLCLSAFAQGFKSEHEAPEKVSLVVKNLDG
                     RVSVVASAEQEKKVTIEAKSAGLPIDSEDIKVQSKGGTFQIDVRPRGEKNRIDIVVTI
                     PIRSKVSVEGQAGAVDVVGSVESAVVKTDTGTIHADVPLEALKFNFVWEASKPRYMSD
                     VELPPIKEKAGGVYKLSGRLGEKDVKKEELVALDFRTQRGVVLLNIDPERAPADLRDR
                     PLTEAARAIVRGGDPSLVEAIRKVSPKMFGDYAKTLPAPDKEPSLLQRTTAPGKLVTA
                     VAPQLMRFNASVTDRNGRAIGGMRESDFTVWENGIERRVTNVAPANEPFNLVLLLDVS
                     GSIEERMDFIRKAARDFLRTTSPQDKISIISFRDDIQVISDFSTDRDLLSRKLDEIDA
                     GGGTALYDALGYILSEQIKRLRGERTAIVVMSDGDDNKSFLPFPAILDALSESGALVY
                     PLYVPSGLIPEGSVPKPEITIDPLRTRYLTLTTRADDEGRKLAAASGGIYYPIRRLED
                     LQKAYDDVVAQLRSAYTITYASNSVSAAPRVRVRTNRDGASVRLSPVVELNTP"
     gene            7235..8236
                     /gene="egtD"
                     /locus_tag="VFH96_01140"
     CDS             7235..8236
                     /gene="egtD"
                     /locus_tag="VFH96_01140"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET6972593.1"
                     /translation="MARTEPTRDSRLYIHNLVKRDPLTGLAEDVRKGLSAQPKRFLPK
                     YFYDELGSQLFEAICLLPEYYLTRAENEILEKYSDQIVAAVEGNKTLVEMGSGSASKT
                     RLIIEALLRRQPELLFMPVDISATALESSSRILLQSYPRLAIEAYAADYFAGLAELGK
                     KPRSRTLALFLGSNISNFDRDEALRFLRAMRSVLNRGDALLLGADLKKDPAILEAAYN
                     DALGVTSAFNLNVLARINRELNGSFDLHNFRHRAFYNESAGRIEIYIESLMDQRVRIE
                     KLDLDVDFAAGELIHTENSYKYDMNGIRALAGQTGFDLSRTWLDSQERFSSNLLLAS"
     gene            8368..9129
                     /locus_tag="VFH96_01145"
     CDS             8368..9129
                     /locus_tag="VFH96_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6972594.1"
                     /translation="MTDPAHKALVEFQNVSFTLSGTNIISDLNLTVERGETLVLLGES
                     GCGKTTTLKLVNRLFEPSSGEVRVEDKTTTTWDPIALRRRIGYVIQEGGLFPHFTIAR
                     NVGLVPALAGWESKRIDERVRELLSLVGLEPDRFAERYPRELSGGQRQRVGVARALAA
                     DPPLLLLDEPFGALDPLTRSSLQREFADLTKRLSKTAILVTHDVREALILARRIGLMH
                     KGKLLLLDTPAQFLASDHPQVRAYLDTLELKGGDQ"
     gene            9126..10679
                     /locus_tag="VFH96_01150"
     CDS             9126..10679
                     /locus_tag="VFH96_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6972595.1"
                     /translation="MSFWQFLQLNWSELLLLVRQHVVLVFIAILIAVAIGVPTGILLT
                     RYRSLRGPVLGLANVMQTIPSLALFGFLIPLPFIGGIGTRTALVALVVYSLLPIIRNT
                     VTGILGVDPNVREAAVAMGMTSSQVLRQVELPLAMGVIVTGIRVATVIAVGVTTIAAA
                     VGAGGLGVYIFRGLRQYDNNLLLAGALSAALLALAADFLLGLLQRQFSFEAKRSVASA
                     TVWKLVLAAAVLIVVVGGFALWRGGASNSGGATARVSVGSKDFTESALLAEIAAQMLE
                     ARGVLVERRFELGGNLPHEALVSGTLDLYPEYTGTSYTAILHHTPISDPRAVYEQVKQ
                     EYASKFNIEVSQPLGFENTFAILVRGDEARRLNLKTISDAAPHTSRWRAGFGQDFMSR
                     ADGYPGFSKSYGLKFAEVREMDLSLTYIALSSRQVDLVAGNSTEGRIATLDLFQLADD
                     RHYFPPYEAVYLVRQDSLTRVPALREVLAKLANGISTDEMRRLNYEIDGNKRDPNAVV
                     KEWLVKRGL"
BASE COUNT         2185 a         2996 c         3116 g         2549 t
ORIGIN      
        1 tacctcgcga ccaatccggc ttcggctgtg ctgcgcgtcg attcagccga cgacgatctc
       61 gtcaaagcga tcctgcaaaa tccggaagtc agcgacgcgg aaccacgacg aacaatcagc
      121 ggccaactca aggctggtcc aatgcagtgg cgaaatctcg tcctgtttgt ggtccaggat
      181 tacggccaca ttcgcgtcag cagactcgtg ccggaaagtg gcgcgtggcc gccggcaaat
      241 ggcgagattt tgatcgagcg tgatgccttt caagtcgcaa gggcaaacat cggcgaggcc
      301 gtgacagtta agacagccaa cggggtcgag cagccactcg tgatcagcgg cagtgttcac
      361 gacgtcggcc aggcgcaggc gcgcatggag aacatcgttt acggctacat caatctcgcc
      421 accctcgtgc aactcggcga acagccgcag ctcaatcaga taaatattct ggtcgcacaa
      481 aacaggttcg acgaagcaca catcagacgg gttgttgccg acgtcgaagc attgatcagg
      541 agtcgcgggc acgaagtgtt gcgcgttgag attccccggc ccggcaagca tcctcattcg
      601 gatctgaccg gcatattgct gctcgcgatg tcgagcttcg gactctttgt gctcgtgctc
      661 agcggtattc tcgtcatcaa cctgctcacg gcgatgatgg cgtcgcaggt gcggcagatt
      721 ggcgtgatga aagcgattgg cggcacgcgc agggcgattg cgggaatcta tttcagccag
      781 gcgctgtttc tcggaatcgc agcggtgatt gtgtcgatac cgttgggcat cctcggcagt
      841 cgcgctcttt gccgctacat ggcctacttt ctcaatttcg acatcaacag cttcgccgta
      901 ccgttctggg tttacctgct cgttgggtta gtcggaattg cggcgccgct gcttgccgcg
      961 gcctatcctg tttggcgtgg aacggtgacg ccggtcagag ttgcgctttc agattttgga
     1021 ctctcgcaga cgaacttcgg cgccagtgct ttcgatcgcg cgctgacacg catcggcggc
     1081 agattcagtt taattgccct cgctattcgc aacagttttc gacgtcgggc gcgactggtg
     1141 ttgacgcttt tgactctgac tgccggtggc ttgttcttcc tggctgcttt gaatgtgcgc
     1201 tcgtcgatga ttaacactct cgaccacatg tttggattga gaaaatttga tctcacggtc
     1261 ggattcgcaa atgcgtacga ggttgaaaag atccaaaagg cagttagcaa cacctcaggc
     1321 gtgaggcgtg ctgaaggttg gtttgtgact gaaggagctc atggcgatga ccgtttgacc
     1381 atcgtcgcat tgccgcctga cacacacctt caggagttcg acatcgttga gggaagaagg
     1441 ctgatgccgg gagacactga cgcgatcgtt atcaacaacg ctcttgccgg tcgagatccg
     1501 aagatgcgag ttggcgaatt ggtgacgctg cgaatcggca cagatgaaaa agtatggcgc
     1561 gtcgctggaa tagcgcgtga ggccttcaat cctacgccag ttggctacat accgctgaca
     1621 ggaatggtaa acagtttgcg gctcgcacta gataacaccg atcccgattc gatcggtgcg
     1681 ttgaagacgg agttggatcg aaacctggag caacaaggta tacgagcgcg tggcagctcg
     1741 acgaagagcg aaagccggtt tgggttcgat cagcacatgt tgatgatcta tgtgtttctg
     1801 attatcacgt cggcgatcat tggtggcgtc ggtggcttgg gtttgatgac gacgatgagt
     1861 ctcaacgtgc tcgagcgacg ccgcgagatg ggagtgatgc gagcgctcgg tgcaacgccg
     1921 cgactgattt ggttgatgat cgtggcggag ggaattgtga tcgggttact gagctggacc
     1981 atcgccgcac tgcttgcttg gccaatcagc aagttggttg gtaattttct ggttcagata
     2041 atgtttcgga gtggcttgga ttttacgttc gagccggtcg gactggtgat ctggttagcg
     2101 gtttcgatca gcttgagtgc gttggcgagt tttctgccgg cgtggaaggc gtcacggacg
     2161 acggtgcgtg aggctctcgc atacgagtaa cgcggatcga aagccggatt ttatccggtg
     2221 cggtgcagga tgaattcggc tatctgctgc aatacggtgg tggggcggtt gatctggttc
     2281 agggatgcga tggcgttgtc gtgcgcgcgt tctgcggcaa ctcgcgtttc ttccaggccg
     2341 tggagggcgg gataggttgc tttgtttgag cgagcgtctt ttccgggagt cttgccgagg
     2401 tcttccgcgg ttgcagtcac atcaagaaag tcgtctgtaa tctgaaataa caatccgaga
     2461 tgcgatccat aactcgtgat cgcgtcgagc tcttcgtcgc tggctttgcc gatgattgcc
     2521 ccgcagcgtg cggctgccac gattagcgcg cctgtcttga gacggtgaat ttcctccagt
     2581 tgcgtgccgc taacctgtcg cgcctcgcct tcgatatcga gtgcctgtcc ggccaccatg
     2641 ccgtgcggcg tcgcggccgc gcgggcgagc tctgagatga ccatgattcg cgtttcaaag
     2701 tttatgcagt cgtcgtcggc gatagtttga aacgcgagag tctggagtgc atcgccggcg
     2761 agaatcgcgg ttgcttcgtc aaaacgcacg tgacacgtcg gccggccgcg tcgcaaatcg
     2821 tcgttgtcca ttgccggcaa gtcgtcgtga atgagtgagt aggtatggac catctcaaac
     2881 gcgcacgccg ttccaatcaa tgcctcgaac ggcacgtcaa aagcctgccc gacggccagt
     2941 agcaacgcgg gacgaaagcg tttgccaccg gcgaacacac tccagcgaat tgcctggtga
     3001 attttttccg gcgccgtggc tgacgacggc agcagctcgt tgagttcgac ttcgacgagc
     3061 gcccgactgc cggccaaaag gctgtcgagc tccaacgagt ttccgagggc agcgtggtat
     3121 ggagttttca ttcttcgcct gttgcttctg cgggcggctc cttgtacgcg gccgcaactg
     3181 gttttccctg attgtcgcgc aacaggatct cgattcgtcg ttcggcctgg ctgagccgtt
     3241 cctggcattc gcgtgaaagc cggatgccct gctcaaacaa ttccaaactt ttctccaacg
     3301 gcaagtcgcc gtcttccaat tgccgcacga tctgttcgag tgcttcaagc gaagcttcaa
     3361 acgtccggcc ctgttctttg ctgttttcca tttttctatt acgacgctat ttatttacga
     3421 cgctggcgct tgcgttgcgg tttcttgagc aacggatcaa cgctgccaat cgcgggcagc
     3481 tctttcatca cattatcgac gatcccttgg accagcacat cgtctttcac ttcaactacc
     3541 tgccattgtt ctgcatcacg ctttagcttc aattcaatgg cccgatcgag catcctggcc
     3601 tcaacggtag caatgtcacc ctccgatttg atagtgacaa acttaggaag tacaaagagg
     3661 tgaccgataa atccttttgg ctttacctct gccgagaaga cagtcagatc cttgactaat
     3721 tcgtcatgaa tcgttttctt caactgcggc aacaacgtcg gcatcaaacc gtcgatacgc
     3781 tgttgaattg aactgttgag cgcgtaacca taccgtgcgg ccgccttctg actcacgctc
     3841 gcggtgatat ttttcgcgac ttcgtcgtcg ttgattagtt ttccaaatgc accgacatcg
     3901 tttcgctgcg ccgcatccac gagtagcgtc acagcataat tcggagtcga ttgatatcgc
     3961 cgccaccaga agaacccacc tatcgctaac cctgcaacgc caatcacaat caacagcgcg
     4021 agaatcgcga gcacacgcgg ccagcgccgt cgtttaccgc cagccctttg tgcgacccca
     4081 cccttcggcc cgtccagatt gattacaatt ctctgccgca tttttgctcg atccgcgtta
     4141 atccgtggct tcgatccgtt cgatccgcgt cctaagcttt cctttttgca accgcacgtt
     4201 cacgacatcg ccttcgctca ccgaagctgc gtcgcgcacg agttttcccg aagcgtcctg
     4261 cgcaatcgca tagcctcgtt gcaatactcc aagcggcgat agagcgtcaa gcgacgccgc
     4321 cgccaaacca agccgttcgc gtccttcctg caacttctcc gcgatcgctc catcgcaggc
     4381 ggacgacgct gcgtcaagtc gtgcgcgtgc gttgttcaaa cgcgatttta cttcatcaaa
     4441 ggcgtgtgag agtgcctgct gttgaactcg cgcacgtaag ttaacgatct gaaatttggt
     4501 gagcctgacg agttgacggc ccgtgtggtc caacgtcgag cagatctgat cttcacgcgc
     4561 tgcgacgatc tcggctgctg ccgaaggcgt tgctgcgcga aagtcggcaa cgaaatcggc
     4621 gatggtgtaa tcagtttcat gaccgactgc cgagatcacg ggaatcgcag acttgcgaat
     4681 cgcgcgcgcc acctcttcct ggttgaatgc ccaaagatct tccgtcgaac cgccgccacg
     4741 tccaacaatc aaaacatcga ccgcgtcttc tggccggcct tcgcgcaatg cccgctcgtg
     4801 ataaagattt atgaaacgaa tcgcacgcgc gatgtcaggg ccggccgcgt cgccctgcac
     4861 ctttgccggt gaaaacaaca cgtgaactgt ccgcgtacgc cgcgagatta cgttcagaat
     4921 gtcgcgaatc gcagcgcccg tcggcgacgt caccacgccg acacggcgcg ggaaaacagg
     4981 caacggccgc ttcaactctt tcgcaaagag tccttctttt tgaagacgat cgcggagttg
     5041 ttcaaacgcg atctgcaacg ctccggcgcc gaccggatcg agtgcatcaa ccacgagttc
     5101 atattcacca cgcggtgcat acacggagag tttcccgcgg gcacgaactt tcaaaccgtt
     5161 cgcgggtcga aaacgaatac gtgtgttggt tgagcgaaaa cacttcgcac gcagttgtgc
     5221 gttttcatct ttgagtgtga agtaccagtg tccggaggag tgatctttga aattcgagat
     5281 ttcaccttcg acccaaacgg cagcaaagcg tccctcgagt gcgattcgga tcgagtttgt
     5341 gagctgcgag acagttaacg gcttgcgctc ggtttcatcg aaaagcgcgt tgaaaagggg
     5401 ttggttcaca ggatttctca gggagtgttt agctccacaa cgggactcaa acgcaccgaa
     5461 gcgccgtcgc gatttgtgcg cactcgcaca cgcggggccg ctgaaaccga attcgatgcg
     5521 taagtaatcg tgtaggccga gcgcagttgg gccaccacgt cgtcgtatgc cttttgcaaa
     5581 tcctcgaggc gacgaatcgg ataatagatt ccgcctgaag ccgccgcgag cttgcgtcct
     5641 tcgtcatctg cgcgcgtggt cagcgtgaga tagcgcgtgc gcaaaggatc gatcgtgatc
     5701 tcgggttttg gcacactgcc ctcaggtatc aaacccgacg gcacatagag cggataaacc
     5761 agcgcgcccg actcgctcag cgcgtccaga attgccggaa atggcaaaaa cgatttattg
     5821 tcgtcgccgt ctgacatcac gacaatcgcg gtgcgctcgc cacgcagacg tttgatttgt
     5881 tcgctcagga tgtaaccaag cgcgtcgtag agtgccgttc cgccgccggc atcgatttcg
     5941 tcaagcttgc gcgagagtaa atcccggtcg gtgctgaagt ctgaaatcac ctgaatgtcg
     6001 tcgcgaaagc tgatgatcga aatcttgtct tgaggactcg tcgtgcgcag gaagtcgcgg
     6061 gcggctttgc gaatgaagtc cattcgctct tcgatactgc ctgacacgtc cagcaacaac
     6121 acgaggttga agggctcgtt cgccggggca acgttcgtaa cgcgtcgctc gatgccgttt
     6181 tcccaaaccg taaaatccga ctcgcgcatg ccgccgattg cgcgtccgtt acgatcggtg
     6241 acactggcat taaaacgcat caactgtggc gctacggcgg tcacgagttt acctggcgcg
     6301 gtagtgcgct ggagcaatga tggttctttg tcaggcgccg ggagcgtttt ggcgtagtcg
     6361 ccaaacatct ttggcgaaac tttgcggatc gcttcgacga gtgaaggatc gccaccgcga
     6421 acaatcgcgc gcgctgcttc agtcaaaggg cggtcacgaa gatccgcggg cgctcgctcg
     6481 ggatcgatgt tcaaaagcac taccccgcgt tgcgtgcgga agtccagtgc gaccagctct
     6541 tctttcttta cgtctttctc gccgagccgg cccgaaagtt tgtaaacgcc gccggctttc
     6601 tctttaattg gaggtagttc gacgtcgctc atgtaacgcg gtttgctggc ttcccaaacg
     6661 aagttgaact tcagtgcttc gagcggcacg tcggcgtgaa tcgtgcccgt gtcagtcttc
     6721 acaacggccg actcaacact tccaaccacg tcaaccgcgc ctgcctggcc ttctaccgag
     6781 accttcgaac gaatcggaat tgtgaccacg atgtcgatgc gattcttttc gccgcgcggg
     6841 cgcacgtcga tctggaaggt gccgcctttc gactgtactt tgatgtcttc cgaatcgatc
     6901 ggcaacccag ccgatttggc ctcgatggtc acctttttct cttgctccgc agacgcaact
     6961 acgctaactc gtccatcaag attcttgacg acgagcgaga ctttttcagg cgcttcgtgt
     7021 tcggatttaa acccctgggc aaatgctgaa aggcacaacc ctgttatcag aaacaaaagc
     7081 tgggcgaggc gttggaacga cattgccgag cgatcttgcc cgcctcagag ctacgggtca
     7141 agcggccacg gatttacacg gatcttttac ggattgatgc tgaattatgc gcgtctgtgt
     7201 agatccgtgg ctgataggct atcatgtccc ggctgtggca aggacagaac ccacccggga
     7261 ttcgaggctg tacatccata acctcgtcaa acgcgatccg ttgacgggcc tcgccgaaga
     7321 tgtgcgcaaa ggattatcgg ctcaacccaa gcgcttcttg ccgaagtact tctacgacga
     7381 actcggctcg cagctcttcg aagccatctg cctgctgcct gagtactact taacccgcgc
     7441 tgagaacgag atcctggaaa agtattcgga ccagatcgtc gctgccgtcg aaggtaacaa
     7501 aacgcttgtc gaaatgggca gtggtagtgc ttccaagaca cgtctgatca tcgaagcgct
     7561 gttgcgtcgc cagccggagc tgttgtttat gcccgtcgac atttcggcga cggcgctcga
     7621 gagtagttca cgaattcttt tgcaatcgta tccgcggctg gcaatcgagg cttacgcagc
     7681 agattatttc gccggcctcg cggagcttgg aaagaagccg cgttcacgaa cactcgcgct
     7741 gtttctcggt tcaaacatca gcaactttga tcgggacgag gcgcttcgtt ttctgcgtgc
     7801 gatgcgcagt gtgttgaatc gtggtgatgc gcttctgctc ggcgccgatc tcaagaaaga
     7861 tccggctatt ctcgaagcgg cgtacaacga tgcgcttggt gtgacctcgg cttttaatct
     7921 caacgtactg gcgcgaatta atcgcgagtt gaacggatcg ttcgatctgc acaactttcg
     7981 tcatcgcgcc ttttacaatg aatcagccgg tcgcattgag atctatatcg agagcctgat
     8041 ggaccagcgc gtgcgcatcg aaaagctcga tcttgacgtt gactttgcgg cgggcgaatt
     8101 aattcatacc gagaattcct acaagtacga catgaacggc atccgcgccc tcgccggtca
     8161 aaccggtttc gatctctcgc gcacctggct cgactcacag gaacgtttca gcagtaacct
     8221 gttgctcgcg agctgatttc tccgtgcaac ctctgtgtcc tctgtgtctc tgtggttagt
     8281 tagccccacg gttagagttc accacagaga cacagagaac acagaggttg cacagagaaa
     8341 tcagttagaa cagactattc aatcttgatg actgaccctg cccataaagc actcgtcgag
     8401 tttcagaacg tcagcttcac actctccggc accaacatca tttcggatct caacttgacg
     8461 gttgagcggg gcgagactct ggtcctgctt ggtgaatcgg gctgcgggaa gacgacgacc
     8521 ttgaagctcg tcaatcgctt gttcgaaccg tcgtcgggcg aagtcagagt tgaagataag
     8581 acgacgacta cctgggatcc gatcgcgttg cgacgtcgca tcggttatgt gattcaggaa
     8641 ggcggtctgt ttccgcattt cacgattgcg cgcaatgttg ggctcgtgcc ggcgctcgct
     8701 ggctgggagt cgaagcgaat cgacgagcgc gtgcgtgagt tgttgagtct ggtgggattg
     8761 gaaccggatc gttttgccga gcgctaccca cgcgagttat caggtggaca acggcagcgc
     8821 gttggagtag cgcgcgccct tgccgccgat ccgccgttgt tactgctcga cgaaccgttt
     8881 ggcgcgctcg atccactcac acgctccagt ttgcaacgcg agtttgccga cctgacgaag
     8941 cgtctaagca agacggcgat cctggtcact cacgacgttc gtgaagcgct gattctcgcg
     9001 cgtcgcatcg gtctcatgca caaaggaaaa ctgctgctgc tcgacacgcc ggcgcagttt
     9061 ctcgcctccg atcacccgca agtgcgcgcg tatctcgaca cgctcgaact caaaggaggc
     9121 gaccagtgag cttctggcaa ttcttgcagc ttaactggtc ggagttgctg ttgttggtgc
     9181 gacagcacgt cgtgctcgtc ttcatcgcga tcctgatcgc agtggctatc ggcgtcccga
     9241 cgggaattct attgacgcgt tatcgatcgc tgcgcggacc ggtattggga ttggcgaacg
     9301 tgatgcagac gattccgagt ctcgcgctat tcggctttct gattccgctt ccgtttatcg
     9361 gcggcatcgg cacccgcacc gccctcgtcg cgctcgttgt ttattcgttg ctgccgatca
     9421 ttcgcaacac agtgaccgga atactcggcg tcgatccaaa cgtgagagag gcggccgtgg
     9481 cgatgggcat gaccagcagt caggtcctgc gacaggtcga gttgccgctc gcgatgggtg
     9541 tgatcgtcac cggcattcgc gtggcaacgg tgattgcagt cggagtgacg acgattgccg
     9601 cggcggttgg cgccggcggg ttgggtgttt acatctttcg cgggctgcga cagtacgaca
     9661 acaatctgtt gctggccggt gcgttgtcgg ccgcgttgct ggcgctggca gcggatttct
     9721 tattggggtt gttgcagcgt caattcagtt ttgaagcgaa acgcagtgtg gcgagcgcga
     9781 ccgtttggaa gcttgtgctg gcagccgcag ttctgatcgt ggtggttggt ggttttgcgc
     9841 tgtggcgtgg tggtgcttcg aactccggcg gcgcgacggc gcgtgtcagt gtcggctcga
     9901 aagatttcac tgagtcggcg ctgctggccg agatcgcagc gcagatgctc gaggcgcgcg
     9961 gcgttttggt cgagcggcgt ttcgagctcg gcggaaatct tccgcacgaa gcgctcgtca
    10021 gtggcactct cgatctctat cccgagtaca ccggaacgtc ttacacggcg atacttcatc
    10081 acacgccgat cagcgatccg cgcgcagttt acgaacaggt caaacaggaa tacgcgtcga
    10141 aattcaacat cgaggtaagc cagccgctcg gtttcgaaaa cacgtttgcg attcttgtgc
    10201 gcggtgacga agcgcggcgg ctaaacctga agacgatttc agacgcagct ccgcatacgt
    10261 cgcgctggcg cgccggtttt gggcaggatt ttatgtcgcg ggctgatggc tatcccgggt
    10321 tttcgaagtc gtacgggttg aagtttgcgg aagtgcgtga gatggatctg tcgctcacgt
    10381 acatcgcact ctcttcacga caggtggacc tggttgccgg caactcgacc gaggggcgaa
    10441 tcgccacgct cgatctcttt caattggcag acgaccgcca ttactttcca ccctacgaag
    10501 ccgtttacct cgtgcgccag gattcgttaa cgcgggttcc tgcgttgcgg gaagtgttag
    10561 cgaagcttgc gaacggaatc tctaccgacg aaatgcggcg gctgaattac gagattgatg
    10621 gtaacaagcg tgatccgaac gcagtggtga aagagtggtt agtgaagcgg ggtttgtaat
    10681 ccacagatta cacagatggc actgtgtaat ctgtggacgt attcagttgt cagcaaggcc
    10741 ctgggatgcc ttggttgtca gtctgagctg ccgccgcctc cagttgttgg tggatcggta
    10801 gccgcccggg cttcggtcat ttcttccgac tcgccgttcg tagtac
//
LOCUS       DASSFQ010000033         7707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_911955, whole genome shotgun sequence.
ACCESSION   DASSFQ010000033 DASSFQ010000000
VERSION     DASSFQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911955"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFH96_01155"
     CDS             <1..209
                     /locus_tag="VFH96_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972596.1"
                     /translation="QGMAWLEQSIKVKETFQNLAAKVNALYKAGKKEEAIALGEQAVQ
                     KGKADKVNTANFEKRLADMKAGKI"
     gene            311..898
                     /locus_tag="VFH96_01160"
     CDS             311..898
                     /locus_tag="VFH96_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972597.1"
                     /translation="MGVAKLTDQNTIVPESKYARIERERRYLLRDLPEGVTRADPHLQ
                     ITDNYITGTRLRIRKVREPRTNKWTVKFTQKFAPDPNDLSRTIITNTYLNALEAEVLS
                     VFDTNEIRKNRYPFEFDGRKFSVDMFLGDLFGLVLAETSFETDEELDTFHKPPFALID
                     VTNDVLFSGGKLCELTFADVRKEILPRISADEKHG"
     gene            895..2046
                     /locus_tag="VFH96_01165"
     CDS             895..2046
                     /locus_tag="VFH96_01165"
                     /inference="COORDINATES: protein motif:HMM:NF025115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HET6972598.1"
                     /translation="MKISILVFCLIISVGCKRAAAPPAPSATTQAQASPGAVLDPAQG
                     GAAPVAQAKYFKGSIGSSLDLQMKLVRTGDQLAGSYFYQKVGTRIDLRGNVDKDGNLT
                     LEEFDKSGKQTGLFKGLWAVDANDGLITLAGNWSKPPNEKDSDKKTAFSVHEEPIAFT
                     GDVDLVAKQIKESNKKLNYEIDARYPQLTGGSNPNFEKFNQVVRASVTKRVAGFRKDL
                     APKEGEEPPPEGSMGSNLGVSYQVMLAQDDLISLEFDIENYYQGAAHPNTFTEVVNYD
                     LKNGKQLKLADLFKPGAKYLQAIANYCIADLKKQAKDKGLLDEEIQNGAAPNAKNYQS
                     WTITRKGLGINFDAYQVGPYAAGPQYVLVPYSALKDVINPEGPVGQFAK"
     gene            complement(2057..3295)
                     /locus_tag="VFH96_01170"
     CDS             complement(2057..3295)
                     /locus_tag="VFH96_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6972599.1"
                     /translation="MKRSPLIVIFTTVFIDLVGFGIVIPVLPFYAEGTAFNATPRMVG
                     LLFASYSIMQLIFSPILGGLSDKYGRRPVLLLSIIGTGIGFLILGLAKTIEMLFIGRI
                     LDGITGGNISTAQAYIADVTTEENRAKGMGLIGAAFGLGFILGPAIGGILSGWGIHVP
                     FIFAAAMCFANAMLLYFTLPETVTADHPAKNLAGGRSLRQVARALKQPKLAFVLVIYF
                     LFIVAFSIMTTSFSLYTMFRFGYDAQHTGYLFAYVGLIAVIIQGGLIGRLVKRFGELP
                     LVIFGALCFAISLFAVPFVGPAAGGLAGLLIGGGVFSMGNSLATPALTSLASKSVGPA
                     QQGIVLGVTQSTASLARAVGPTIAAVLISSSIAHLGADGLSHYMSDRSLFVTFWTGAA
                     IMLLATFLAFYYSKIRAVYP"
     gene            complement(3309..3770)
                     /locus_tag="VFH96_01175"
     CDS             complement(3309..3770)
                     /locus_tag="VFH96_01175"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6972600.1"
                     /translation="MFRRIIATSPAWFTVPVRLGLAAVMIAHGSQKVLGSFNGPGFKT
                     YVSGTTPIGFMRPAWLWLGAAAFSEFVGGILVGLGFLTRVGAFFIACVMVTAIAGVHW
                     PGGFFAANRGYEYPLTLLAMAIALLISGGGQASVDRALSGGGGGGRGRGRR"
     gene            3861..5714
                     /locus_tag="VFH96_01180"
     CDS             3861..5714
                     /locus_tag="VFH96_01180"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6972601.1"
                     /translation="MHKSLVLCAAALVLVFPILAAAQAPVDISFTVAMSKPHTHLFEV
                     DVAIKRTANGPQEELLVMPVWTPGSYLIREFERNVQDFAAADAAGQPLKWEKTNKNTW
                     RINTNGAREWHATYRVYANELSVRTSELNSEHAFWNNANLLMYLEGFLKSPSTVHVLA
                     PDVWKVATGLPAVAGQKNTFRAENFDVLYDSPFEASNFKTLVFNVKGVPHRIVIDGAG
                     NYDPERMRADVQKIVETEVEIMGGEIPYRDYTFILHLRQNAGGGLEHLNSTALGYPRF
                     GFHVRSGNRATSAAPNTIDTSERDYRGFLGLVSHEFFHLWNVKRIRPDALGPFDYTQE
                     NYTKVLWVAEGITDYYADLVLRRAGLISESEYLSAQARAMQSLQNTPGRLVQSAEESS
                     FDAWVKYYRQDENSVNSQVSYYDKGALLGLLLDLEIRKRSAGAKSLDDVMRYLYAEFF
                     KKNRNYGPADFQKAVELMAGGSLEEFFSKYVRGVQELDYNAALDAAGLKLDTTAATNA
                     KVYFGADVAEDEGRLMVRRVYAGSPAYEQGLNAGDEIVAMDNMRATRDFFNARMAEKK
                     PGDLVNLTIFRFDDLSSLLIRLGDKREMSFRIVPVTTATAVQKQIYANWLR"
     gene            5796..6563
                     /locus_tag="VFH96_01185"
     CDS             5796..6563
                     /locus_tag="VFH96_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6972602.1"
                     /translation="MAEHIHFRIDDRVARLTFAKPPLNVLNIAMMREITSTLAECPHR
                     ELVAIVIDADKNCRAFSAGVSIEEHAQETIFQMLDAFHSIFRLLEQLAKPTIAVVDGA
                     ALGGGCELVAACDIVIASDRSRFGQPEIKLGVFPPVAAVLLPRVIGEKRARELILTGE
                     IIDAVEAGRLGLCNHVVPGAHLEPKLLEVLAKLRELSGTSLAYARQSLDLGRGRSIEA
                     ALDEQENIYLQELMKTHDANEGVKAFMEKRKATWRHR"
     gene            6567..7418
                     /locus_tag="VFH96_01190"
     CDS             6567..7418
                     /locus_tag="VFH96_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972603.1"
                     /translation="MKKFFLVTLLLVCLSPAAAQTKSGQRAHASMSPEAKALLEQAIG
                     VVCTQAKLDPKSSIAIDEMQSRPSLPLHSPEARAGAERAQRLLPVAKDLVISSLRQLT
                     TEYGVQQSRSFNLRLTQAIARVNSVKRVRPDMDSRDNASVFLSRPHVITFGTIFLAGL
                     RSDEGMISVLAHELMHIADGDNDSLRTLVRAVGNRASDLTGLDIHGQRSEEITCDLIG
                     AMATRAYIVNSPDYESIARRLARSIQHNCVDLDEGDEDHLSPRNTIRALLALNPGLVR
                     ELINDRF"
     gene            7478..>7707
                     /locus_tag="VFH96_01195"
     CDS             7478..>7707
                     /locus_tag="VFH96_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972604.1"
                     /translation="MVLFLFIFVAGCKNVVERQNVTPRVLRDVPARNLAYRLSPDVTP
                     PSTEIDDTLDKFAAVANAFSTTRKDEALLRTVV"
BASE COUNT         1832 a         2038 c         2267 g         1570 t
ORIGIN      
        1 cgcagggcat ggcgtggttg gagcagtcga tcaaagtgaa ggagacgttc caaaacttag
       61 ccgccaaggt gaacgcgttg tacaaggccg ggaagaagga agaggcgatc gcgttgggcg
      121 aacaggccgt gcagaaaggc aaggccgata aagttaatac ggcaaatttc gagaagcgac
      181 ttgccgacat gaaggccgga aaaatctaga agtacacaaa aggcacaaaa gaacaaaagg
      241 cacataaaat cagaaaagct atttatgtgc cttttgttct tttgtgcctt ttgtgtactc
      301 ttttttgtct atgggtgtag cgaagcttac tgaccagaat acgattgttc cggagagcaa
      361 gtatgcgcgg atcgagcgtg agcgccgata tttgttgcgc gatttgcctg aaggcgtgac
      421 gcgcgcggat ccgcatctac agatcaccga caactacatc accggcacac gcctgcgcat
      481 tcgcaaagtg cgtgaaccgc ggaccaacaa gtggacggtc aagttcacac agaaattcgc
      541 gcccgatccg aacgacctct cgcggacgat aatcaccaac acctatctca acgcgctcga
      601 agctgaagtt ttgtcagtct tcgacacgaa cgagatacgc aagaaccgtt acccgttcga
      661 gtttgacggg cggaagtttt ccgtcgacat gtttctcggc gatctgtttg gactcgtgct
      721 cgccgaaacg agcttcgaaa ctgacgaaga gctcgatacc ttccataaac ctccgtttgc
      781 gttgattgat gtcactaatg atgtgttgtt tagtggtggg aaattgtgtg agctgacgtt
      841 tgccgatgtt cggaaggaaa ttttgccgcg gatttccgcg gatgaaaaac acggatgaag
      901 ataagcatac ttgtcttttg tttgatcatt tcggttggtt gtaagagggc ggcagcgcca
      961 ccggccccga gcgcaacaac gcaggcgcag gccagtccgg gggcggtgct cgatcccgcg
     1021 cagggcggcg cggccccggt cgcccaggcg aaatacttca aaggttcgat cggctcttca
     1081 ctcgacttgc agatgaaact tgtgcgcacg ggcgatcagc tggccggcag ttacttctac
     1141 cagaaggtcg gcacgcgtat cgatctgcgt ggcaacgtcg ataaagacgg aaacctgacg
     1201 ctcgaagagt ttgataagag cggcaaacag acgggcttgt tcaaagggtt gtgggctgtt
     1261 gatgcgaacg acgggctcat cacgctcgcg ggcaactggt ccaagccgcc aaatgaaaaa
     1321 gacagcgaca agaagaccgc gttctcggtt catgaagagc cgatcgcctt cacgggcgac
     1381 gtcgatctgg tagcgaagca gatcaaggaa agcaacaaga agctgaatta tgagatcgac
     1441 gcgcgctacc cgcagctcac ggggggcagc aatccgaatt tcgagaagtt caatcaggtg
     1501 gtgcgcgcgt cagtcacgaa gagggtggcg gggttcagaa aagacctggc gcccaaagag
     1561 ggtgaagagc ccccacccga aggatcgatg ggcagcaatt tgggagtgag ttaccaagtg
     1621 atgctggcgc aggatgatct gatcagtctc gagttcgata ttgagaatta ttaccagggc
     1681 gccgcgcatc cgaacacgtt tactgaggtg gtgaactacg atctcaagaa tggaaaacag
     1741 ctcaagctcg cggatctctt caagcccggt gcgaaatatt tgcaggcgat cgcaaactac
     1801 tgcatcgcgg atctgaagaa gcaggcgaaa gacaagggcc tcctcgacga agagattcag
     1861 aacggcgccg caccaaacgc gaagaattat cagagctgga ccatcaccag gaaaggtttg
     1921 gggatcaact tcgacgccta ccaggttggc ccatacgccg ccggcccgca gtacgtgctc
     1981 gtgccgtact cggcgttgaa agacgtgatc aatcccgagg ggcctgttgg tcaattcgca
     2041 aagtaagcca cggattttac ggataaacag cgcggatttt gctgtaataa aaagcgagga
     2101 aagttgccag cagcatgatg gcggcgccgg tccagaaggt cacgaagagc gagcggtcgc
     2161 tcatgtagtg cgataaaccg tcagcaccga gatgcgcgat cgagctgctg atcaatactg
     2221 ccgcgatagt cggtccgact gcgcgtgcga ggcttgcagt tgactgtgtg acgcctaaga
     2281 caatgccctg ttgcgccggt ccgacactct tcgacgccag actcgtcagc gccggtgtcg
     2341 ctaacgaatt acccatcgaa aacacaccgc cgccaatcaa caaccctgcc aacccgccgg
     2401 ccgctggtcc aacaaacggc actgcaaaca aactgatcgc aaaacacaac gcaccgaaga
     2461 ttacgagtgg cagctcacca aatcgcttca cgagtcgccc gatcaatccg ccctgaatga
     2521 tcaccgcgat caagccaaca taagcgaaga gatatccagt gtgttgcgcg tcgtaaccaa
     2581 agcgaaacat cgtgtacagc gagaacgacg tcgtcatgat cgagaacgcc acgatgaaca
     2641 ggaaatagat cactaacaca aacgcgagct tcggttgttt cagcgcgcgt gcaacttgcc
     2701 gcaggctgcg accaccggca aggttcttcg ccggatgatc cgccgtcact gtttcaggca
     2761 atgtgaaata cagcagcatc gcattcgcga agcacatcgc cgccgcaaat ataaaaggaa
     2821 catgaattcc ccagccgctg agtatgccgc cgatcgctgg tccaagaatg aagccgaggc
     2881 caaatgccgc gccgatcagg cccattcctt tggcccgatt ctcttcggtc gtgacgtcgg
     2941 cgatgtaagc ctgcgcggtc gagatgtttc cacccgtgat gccgtcgagg atacggccga
     3001 taaacaacat ctcgatcgtt tttgcgagcc cgagtatgag aaaaccgatg cccgtgccga
     3061 tgatcgatag caacaacacc ggcctgcgac cgtacttgtc agacaagccg ccgaggatcg
     3121 gcgagaagat gagttgcatg atggagtagg aggcgaagag cagcccaacc atgcgcggcg
     3181 tcgcgttgaa ggcggtgcct tcggcgtaga atggcagcac cgggatcacg atgccaaagc
     3241 ccacgagatc gataaagacg gttgtaaaga tgacgatgag cggtgagcgc ttcatgttga
     3301 tagcggattt atctgcgtcc cctgccgcga ccgccgccgc ctcccccgct cagggctcga
     3361 tcgactgaag cctggccgcc gccggatatc agcagcgcaa tcgccatcgc cagcagagtc
     3421 aacggatact cataaccacg gttagcggcg aagaagccgc cgggccagtg cactcccgcg
     3481 atcgccgtca ccatcacgca ggcaatgaag aacgcgccca cacgcgtgag aaaaccgaga
     3541 ccgacgagta ttccgcccac gaattcggaa aacgctgcgg cgccgagcca cagccatgcc
     3601 ggtcgcatga aaccgattgg cgtggtgccg ctgacgtagg ttttgaagcc cgggccgttg
     3661 aagctgccca gcactttttg cgagccgtgg gcaatcatta cggccgccaa tcccaaacgg
     3721 accggcacgg taaaccaggc tggagacgtg gcgatgattc gtctaaacat ttcgatctcg
     3781 tctttgatgt ggattacaag agcgcgaaga tagtaacatt cgttgacagg attacaagat
     3841 taacaagatg aacaagattc atgcataaga gcttagtact gtgcgccgcc gcgctggttc
     3901 tcgtattccc gattttggcc gcggcacagg cgcccgttga catctcgttt acagtggcga
     3961 tgtcgaagcc gcacacacac ctgtttgaag tcgacgtcgc gatcaagcgc acggcgaacg
     4021 gtcctcagga agagctgctc gtcatgcctg tctggacgcc cggttcttac ctgatccgcg
     4081 agtttgagcg gaacgtgcag gactttgccg ctgccgacgc cgcgggccag ccgctcaaat
     4141 gggaaaagac aaacaagaac acctggcgca tcaacaccaa cggtgcccgc gaatggcatg
     4201 cgacgtatcg tgtttacgcg aacgagcttt cggtgcggac gagtgagcta aactcggaac
     4261 acgccttctg gaacaacgcc aacctgctga tgtatctcga aggttttcta aagtcgccgt
     4321 caaccgtgca tgtgctcgcg cctgacgttt ggaaagtcgc gactggattg ccggcggttg
     4381 ccggacagaa gaacactttt cgcgccgaga atttcgacgt cctttacgac tcgcctttcg
     4441 aagccagcaa cttcaagacg cttgtcttca acgttaaagg tgttccgcat cggatcgtga
     4501 tcgatggagc ggggaattac gatccggaac ggatgcgagc cgacgtgcag aagatcgtcg
     4561 agacggaagt ggagataatg ggcggcgaga ttccgtatcg cgattacacg tttattctgc
     4621 atctccgaca aaacgcggga ggcggactcg agcatctcaa ttcgactgcg ctcggttacc
     4681 cgcgtttcgg atttcatgtc cgcagcggca accgcgccac gtctgcggcg ccgaatacca
     4741 tcgacacgtc cgagcgtgat tatcgcggct tcctcggtct cgtctcgcac gagttttttc
     4801 atctgtggaa cgtgaagcga attcgtcccg atgcgcttgg gccatttgat tacacgcagg
     4861 aaaactacac gaaggttctg tgggtcgctg aaggtattac tgattactat gcagatctgg
     4921 ttttgcgtcg cgcgggactg atcagcgaga gcgagtatct gagtgcgcaa gcgcgcgcga
     4981 tgcaaagctt gcagaacacg ccgggtcggc ttgtgcagag tgctgaggag tcgagttttg
     5041 atgcatgggt gaagtattac cggcaggatg agaactcggt aaactcgcag gtttcgtatt
     5101 acgacaaggg cgcgttgctc gggttgctgc tcgatctcga gatacgaaag cgaagtgctg
     5161 gagcgaagtc actggacgac gtgatgcggt atctttatgc ggagtttttc aagaagaacc
     5221 gcaactatgg accagcggat tttcagaaag ctgtagagct gatggccgga gggagtctcg
     5281 aggagttttt cagtaagtac gtgcgtgggg tacaggagct ggattacaac gcggcgctcg
     5341 atgcggcggg gttgaagttg gacacgactg cggcgacaaa tgcgaaagtg tatttcgggg
     5401 cggacgtagc cgaggacgaa ggccggttga tggtgcggcg tgtttacgcg ggctcaccgg
     5461 cgtacgagca gggattgaac gcgggcgacg agatcgtggc gatggataac atgagagcga
     5521 cgagggattt cttcaacgcg cggatggcgg agaagaagcc gggcgacctg gtgaatttga
     5581 cgatcttcag gtttgatgac ttgagctcgt tgctgatccg gttgggtgat aagcgtgaga
     5641 tgagttttag aatcgtgccg gtgacgacgg cgacggctgt gcagaaacag atctatgcta
     5701 attggttgag gtagttgtta gacaggattt acaagatctt tcaggattaa catgtaaatc
     5761 ctgctcaatc ttgtaaatcc tgtctgagac tgacgatggc agaacacatt catttcagaa
     5821 ttgacgatcg cgtagcgcgg ctcacgttcg caaagccacc gctgaacgtg ctcaacattg
     5881 ccatgatgcg tgagatcacg agtacgctcg cagagtgccc gcatcgtgag ctcgtggcga
     5941 ttgtgatcga tgctgacaag aattgtcgcg cgttcagcgc gggtgtttcg atcgaggagc
     6001 acgcgcagga aacgatcttc cagatgctcg acgcctttca ctcgatcttt cgtctcctgg
     6061 aacagttagc gaagccgacg atcgcggtgg tagacggcgc ggcgcttggt ggtggctgtg
     6121 aactcgttgc ggcatgcgat atcgtgatcg cgtccgatcg ctcgcgattc gggcagccgg
     6181 agataaagct cggcgtgttt cctcccgttg ctgccgtgct cttgccgcgc gtgatcggtg
     6241 aaaaacgcgc gcgcgaattg atcctgacgg gtgagataat cgatgcggtt gaagccgggc
     6301 gtttgggttt gtgcaatcac gtcgtccccg gcgcccatct cgaaccgaag ctgcttgaag
     6361 ttttagccaa attaagagaa ctgtcgggca cgtcgctggc gtatgcaaga cagtcactgg
     6421 accttggtcg cggccggtcg atcgaagccg cgctcgacga gcaggaaaac atttacctgc
     6481 aagagctgat gaagactcac gacgcgaacg aaggtgtgaa ggcgttcatg gagaagcgca
     6541 aggcgacctg gcgccaccgt tagacaatga agaaattttt cttagtcacc ctcctgctcg
     6601 tttgcctttc cccggctgct gcgcaaacca agtccggaca gcgtgcgcac gcctcgatgt
     6661 cgcccgaagc gaaggcgctg ctcgaacaag cgatcggagt tgtttgcaca caggccaagc
     6721 tcgatccgaa gagcagcatc gcgatcgacg agatgcagtc gcgcccgtca ctcccgctcc
     6781 acagtcccga agcacgcgcc ggcgccgagc gcgcgcaacg acttttgccc gtcgcgaaag
     6841 acctcgtcat cagctcgctg cgacagctca caaccgagta tggtgttcag caatctcgtt
     6901 ctttcaacct ccggctgaca caggcaatcg cgcgtgtgaa ttcagtgaag cgcgtgaggc
     6961 ctgacatgga ctcgcgcgac aacgcctcgg tctttctaag ccgtccgcac gtgataactt
     7021 ttggcacgat cttcctcgcg ggcttgcgtt ctgatgaggg aatgatcagc gtcctggcgc
     7081 acgagctgat gcacatcgct gacggtgata acgacagttt gcgtacgctc gtccgtgcgg
     7141 ttggcaacag agcttccgat ctaacagggc tcgacattca cgggcagcgc agcgaagaaa
     7201 tcacgtgcga tttgatcggc gcgatggcga cgcgcgcgta catcgttaac tcgcctgatt
     7261 acgagtcgat cgcgagacgt ttagcgcgct cgatccaaca caactgcgtc gatcttgatg
     7321 aaggcgacga agatcatctg tcaccgcgta acacaattcg cgcgttgctc gcgttgaatc
     7381 ccggtttggt gcgcgagttg atcaacgatc gattttgatg ccacaaagaa gcacaaatgg
     7441 aagatctttg ccgcggatct tcgcggataa acgcggattg gttctctttc ttttcatctt
     7501 tgtggccggg tgcaagaatg tcgtcgagcg acagaatgtg acgccgcgcg tcctgcgtga
     7561 cgtgccggcg cgtaatctcg cgtatcgact ctcgcccgat gtcacgccac cctcaactga
     7621 gattgacgac acgttggaca agtttgcggc cgtcgcgaac gccttcagca cgacgcgcaa
     7681 ggatgaagcg ttgctgcgaa cagtcgt
//
LOCUS       DASSFQ010000034         9814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2101095, whole genome shotgun sequence.
ACCESSION   DASSFQ010000034 DASSFQ010000000
VERSION     DASSFQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101095"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="VFH96_01200"
     CDS             <1..1959
                     /locus_tag="VFH96_01200"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6972605.1"
                     /translation="SYVETLSYVNGAYEFVFPMVVGPRYVPGSPKAQSQGAGFAPDTK
                     QVPDASRITPKSPPAGMRSGHDISLEIGLDAGLIIDNIDSKTHPTDIQRPDAHSARLR
                     LKDNETIPNKDFVLRYDVAGQAIQDALLTHRSDKGGFFTLMLQPPERVTAQDVTPKEL
                     VFVLDTSGSMEGFPIEKAKETMKLALDSLYPSDTFNLITFAGDTRILFKQPVPATRAN
                     LNKAQAFLRASTGEGGTEMMTAIKAALEPSDDQSHVRIVCFMTDGYVGNDMEIISEVQ
                     KHQNARVFSFGIGSSVNRFLLDKMAEAGRGEVEYVDLDDDGSAAARRFHERIRNPLLT
                     DIAIEWNNLPVADVYPQRIPDLFGAKPVVIKGRYTGAGRGTIRLKGKMAGGDFVRDIP
                     VEFPETMASNDVLATLWARARVDDLMGQDFKGAQSGSMRPDLKDTITQLGVEYRLLTQ
                     FTSFVAVEEMVVTDGGKPRRIDVPVEVPAGVKGDSDAEPDGRRYLSFALTRAGTNLVR
                     SKARGSGGGGSARVYPSPPNQPPTVNSVTASVGDVISSEQRTLPEPVSAELQSKLHRS
                     VLAVVQKLRKKELLLGSEGDFIRDGKAEVQVWLTDKSNETLAQLKELGFEIVLDAKSA
                     NMLVGRLPIEKLEMLAKLKSVKYVAPQR"
     gene            2089..3420
                     /gene="lysC"
                     /locus_tag="VFH96_01205"
     CDS             2089..3420
                     /gene="lysC"
                     /locus_tag="VFH96_01205"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HET6972606.1"
                     /translation="MKFGGTSVADATAIENVARIVARERAAFPVIVVSALSGVTDALL
                     AATHASVAAIFSRHLATAEELLGVSESFVALVKSAETQVRALLQSQSPSNRKAVQDSI
                     VSFGEILSSHLVAAVLNQRGVEARQVDARRCIITDEEHTSAAPLLRESFKRTEDELRA
                     LVESGVVPVLGGFIGATLQGATTTLGRGGSDYTAALIGAALKVDEIQIWTDVTGVLTA
                     DPRVVPEAQTIDRLSYSEAAELAYFGAKVLHPKTIQPAIENSIPVRICNSRLPQERGT
                     LVGPQSETSPRTIKAIAHKTGVTVVQVTSLRMLGAYGFLRALFEVFDRHRVVIDVVTT
                     SEVSVSLSLDEAGALPAIVEELQQLGTVGIEKGRAIICVVGEGLRGTAGIEARVFSTI
                     SDINVSLISQGASSINFTFVIEEERVKEAVTRLHEEFFSHEKAQKAQKEDL"
     gene            3421..4419
                     /locus_tag="VFH96_01210"
     CDS             3421..4419
                     /locus_tag="VFH96_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6972607.1"
                     /translation="MNVCELTRQLIDIPSVTGEELAVGEFLARHLEELGYRVERQEVA
                     TDRFNVIATTAALPHFVFSTHMDTVPPFIGSSEDEEFIYGRGSCDAKGIIAAQIFAAQ
                     RLRDGGMNNVGLLFTVDEEMASLGAQVANKHPIAKSCKFLINGEPTDNRLATGTKGSV
                     RVIVTTEGRAAHSAYPEAGESAIEKLLDILQDIRAIQWPGDSFFGTTTCNIGVLSGGT
                     RPNVIADKARAELQIRLGIDIEHIKRKLEDAVGTRGRPEYSSAHNPVRLFSVPGFEEC
                     VVRFTTDVPYLSEWGKPLLLGPGSIFDAHTDHERISKRELEQAVDLYLRLSKQLIP"
     gene            4416..5120
                     /locus_tag="VFH96_01215"
     CDS             4416..5120
                     /locus_tag="VFH96_01215"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET6972608.1"
                     /translation="MKLALFGYGAMGQLVAAGARKGGDEIGVILTSKDDLPVDQLAEK
                     LSGHDVAIDFSIGDAVIKNIEVCALAHVPLVEGTTGWKQHESTAKQLITEHSGAMVYG
                     ANFSIGVNLFYRITKYAAALFSAVDGYAPFIEEAHHNRKRDAPSGTALKLRDLMSERL
                     GPDIPTSSTRAGYIPGTHRVGFDSEADQILLTHTARSRQGFANGALLAAHWIVGRTGV
                     FEFSDVIEEIITTKST"
     gene            5174..6097
                     /gene="dapA"
                     /locus_tag="VFH96_01220"
     CDS             5174..6097
                     /gene="dapA"
                     /locus_tag="VFH96_01220"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET6972609.1"
                     /translation="MTIQLDWLRGCATALVTPFTASGAVDEPRLRDLIEYQITGGVRI
                     LVPCGTTGESVTMTDEENRLVISTAVELARGRAKVIAGTGSNSTAVTIQRSQNARALG
                     VDGVLTVAPYYNKPTQDGLYAHFRAIAEAVSDLPVVIYNVPGRTSSNISADTTLRLAR
                     DVENIVAVKEASGNLSQIMEILRSRPEGFRVISGDDSLTLALIALGGEGLISVASNEA
                     PEMMSRLNELALAGQWDEARALHYRLLPLMEGNFIESSPGPVKAGLALMGMIEENLRL
                     PLVPVQEKTRNRMREILTEIGLLKETSHAVA"
     gene            6084..6917
                     /locus_tag="VFH96_01225"
     CDS             6084..6917
                     /locus_tag="VFH96_01225"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET6972610.1"
                     /translation="MLLREQIEALAARADNQFSCADRAVFDELKRALNRGEVRAAEKN
                     SDGKWIVNSWVKQGILVGFRMGAIVDMSVGASFKFFDKDTYPGRPTAIEDNVRIVPGG
                     STIRDGVYIAPGVVCMPPMFVNAGAYVDEGTMIDSHALVGSCAQIGRRVHISAAAQIG
                     GVLEPVGAVPVIIENDVLVGGNCGVYEGTIVRERAVLASGTILTGSTPVYDLVRGEIY
                     QRASDGPLEIPAGAVVVPGARAVTTERGREWGLSLYAAVIVKYRDEKTDTAVRLEDYL
                     R"
     gene            6914..8065
                     /locus_tag="VFH96_01230"
     CDS             6914..8065
                     /locus_tag="VFH96_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6972611.1"
                     /translation="MNKFAPPKRLQGIEKSVIRQVFDRAQPGSLNLGLGEPDLPTPDV
                     IRRAAVAAIVEEQNGYTSHAGLPALREKVANEYPYLEGRPERVVITAGSQEALYLALL
                     TLVDEGDEVLLPNPGFVAYPTIVRMAGGTSRFYRLPREHGFAFDIDEFRRTLTPQTKV
                     VVCISPSNPTGRILTNEDLHAIANTLRDHGAYLISDEIYRDLYYTDERPGSLSSFSDR
                     TIVISGLSKSMSMTGWRLGWVCGAEELIKAMLVLHGYVTTCASTVSQKASLVAWSDEA
                     EEARAGFRDTFRARRDHLLGLVESELRLKAVTPDGAFYTMLDVSEYGSSMKVAEALLE
                     EKVITVPGAAFGSEGEGFLRVSFCADKEMLDEGVQRIKKAIHRLHRKNL"
     gene            8105..9151
                     /gene="asd"
                     /locus_tag="VFH96_01235"
     CDS             8105..9151
                     /gene="asd"
                     /locus_tag="VFH96_01235"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET6972612.1"
                     /translation="MKKYRVGILGATGMVGQRFIQLLEHHPQFEITAVAASDRSQGKT
                     YGEACTWRLNGEMPVAVRSLPVQPPQPPLDCDLVFSSLPGDIARETEGSFANAGYPVI
                     SNSSAFRMDEDVPLLIPEVNHQHLGLLARQRRTNTSGGFIVTNPNCSTIMIALALAPL
                     HATFGITSTVATTMQALSGAGYPGVASLDIIDNVLPHIGGEEEKIETETTRILGRFMN
                     DAIEPASMAVSAQCHRVNVADGHMAAVRVNFTRKPALPDLREALTSFRSLPQELGLHS
                     APANPIVVRDEQDRPQPKLDRDAGNGMTITVGRLKEDTVLDYRFVVLSHNTIRGAAGA
                     AILNAELLIALGQL"
     gene            9176..9337
                     /locus_tag="VFH96_01240"
     CDS             9176..9337
                     /locus_tag="VFH96_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972613.1"
                     /translation="MNKIRFLIPLLFLFSISVQAQTNLDDQVKPIVASFKGKVSLFAR
                     NSTQGRRTD"
     gene            9334..>9814
                     /locus_tag="VFH96_01245"
     CDS             9334..>9814
                     /locus_tag="VFH96_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6972614.1"
                     /translation="MNPDERVRTASTIKIAVMIEAFARVAEGKAKWTDEVILTKEKKV
                     SGSGILNELSDGLKLTLRDAVNLMMMISDNTATNLVLDVLTTDAVNARMESLGFKQIK
                     IMRKVGSGGESTAGKDPENKKYGLGMATPREMVLVMEKLERGEVISPAASKEMIDLMK
                     "
BASE COUNT         2206 a         2529 c         2972 g         2107 t
ORIGIN      
        1 agctacgtcg aaacgttgag ctatgttaac ggcgcgtatg aattcgtgtt cccgatggtg
       61 gttggaccac gttacgtgcc cggttcacca aaagctcaat cgcagggcgc tggcttcgct
      121 cccgacacga agcaggttcc cgacgcctcg cgcatcactc ccaagtcgcc tcctgccgga
      181 atgagaagtg gtcacgacat ctcgctcgag atcgggctcg acgccggttt gatcatcgat
      241 aacattgatt ccaaaactca ccccacagac attcaacgcc cggatgctca cagcgcgcga
      301 cttcgtctca aagacaacga aactattccc aacaaagact tcgtgcttcg ttacgacgtc
      361 gccggccagg cgatccagga cgcactgctc acgcatcgtt ccgacaaggg aggtttcttc
      421 acgctcatgc tgcaaccgcc cgagcgcgtg accgcgcagg acgtgacgcc aaaagagctc
      481 gtgtttgtgc tcgacacgtc cggctcaatg gagggatttc caatcgagaa ggcgaaggag
      541 acgatgaagc tcgcactcga tagtctctat ccgtctgaca cgttcaatct gatcacgttt
      601 gcaggcgaca cgcgcatatt gtttaagcag ccagtgccgg caacacgcgc gaacctcaac
      661 aaggctcagg catttcttcg tgccagcacc ggcgagggtg gcaccgagat gatgacggcg
      721 atcaaagccg cgctcgagcc gtctgacgat cagtcacacg tccgcatcgt ttgcttcatg
      781 actgacggtt acgtcggtaa cgacatggaa atcatttccg aagttcagaa gcaccagaat
      841 gcgcgcgtgt tttcgtttgg aatcggaagc tcagtgaacc ggttcctgct cgacaagatg
      901 gcggaagcag gacgcggcga agtggaatac gtcgatctag atgacgacgg ctctgccgcg
      961 gcgcgtcgct ttcacgaacg catacgcaac ccgctgctga ccgatatcgc gatcgagtgg
     1021 aataacctgc cggttgccga cgtctatccg caacgcattc cggatctctt cggcgctaag
     1081 ccggtggtta ttaaaggacg ttacacgggc gcgggccgcg gcacgattcg actgaaagga
     1141 aagatggccg gcggtgattt cgtgcgcgac attccggtcg agtttccgga aacgatggcg
     1201 tcgaacgatg tgctggcgac tttgtgggcg cgtgcgcgag ttgacgatct catgggccag
     1261 gacttcaagg gcgcgcaatc aggcagcatg cgaccggatt tgaaagacac gatcacacag
     1321 ctcggcgtcg agtatcgttt gctgacgcag tttacttcgt tcgtcgcggt cgaggagatg
     1381 gtggtaacag atggcggtaa gccacgacga atcgatgtgc cggttgaggt tccggccggc
     1441 gtaaaaggag actcggatgc agagcccgac ggtcgcaggt atttaagttt cgcgcttacc
     1501 agagcgggca cgaatttagt tcgaagcaag gcgagagggt ctgggggcgg aggcagtgct
     1561 cgggtatatc cgtcgccacc aaaccagccg cccactgtga attccgtgac cgcgtccgta
     1621 ggcgatgtga tcagttccga gcagcgtacg ctgccggagc cggtaagcgc tgagctgcag
     1681 tcgaaacttc atcgctcagt gctcgcagtc gtccagaagc tgagaaagaa ggaactactt
     1741 ttgggcagtg aaggagattt cattcgcgac ggtaaggcgg aagttcaggt ctggttaacg
     1801 gacaaatcta acgagactct ggcgcaactc aaagagctcg gttttgaaat tgtgctcgat
     1861 gcgaagagcg caaacatgct cgttgggcgt ttgccgatcg agaagctcga gatgttggcg
     1921 aaacttaagt ctgtgaaata cgtcgcgccg cagcgataga ttcaaagcaa atgtccgata
     1981 aacttcagtt tgtcgcgcaa tccgacaagc taaagcatgt tggacatcta agccacagag
     2041 ttcatggaca acacggatcc gtgtaatctg tggcgagtgt caatcgtgat gaaatttggc
     2101 gggacgtcag tagccgacgc cactgccatc gagaacgtcg cacgcattgt tgccagagag
     2161 cgtgcggcgt ttcccgttat cgtagtttcg gcgctgagcg gagtgactga tgctttgttg
     2221 gcagcaacgc acgcatcagt tgcggcgata tttagtcgac atttagcaac cgcagaagaa
     2281 cttctcggag tgtctgagtc ttttgtcgcg cttgtaaaaa gcgctgaaac tcaggtgcgc
     2341 gccttactgc aatcgcagag cccttccaat cgcaaagctg ttcaagattc gattgtctcg
     2401 tttggcgaga tcctctcgag ccatctggtg gccgcggtgt tgaatcaacg cggagtcgaa
     2461 gcgcggcagg ttgatgcgcg tcgctgcatc atcacggatg aagagcatac gtccgctgcg
     2521 cctctgctgc gggagagttt caagcgcacc gaagatgagt tgcgagcgct cgttgaaagt
     2581 ggagttgttc cggttctggg tggattcatc ggtgcgacat tgcaaggcgc gacgacgacg
     2641 ctcggacgcg gcggctctga ttacaccgca gcgctgatcg gcgccgcgct caaagtcgat
     2701 gaaatacaga tctggaccga cgtcaccggc gtgttgactg ccgatccacg tgtggttccc
     2761 gaggcgcaga cgatcgatcg tttgtcttac agtgaagcag ccgagcttgc ttacttcggc
     2821 gcgaaggtcc tgcatccgaa aacaattcag cctgcgattg aaaattccat tccggtgcgc
     2881 atctgcaatt cgcgcctgcc gcaggagcga ggcacgctgg ttggcccaca aagcgaaacc
     2941 tcgccgcgca cgatcaaggc gatcgcgcat aaaacgggtg tgacggtcgt gcaggttacg
     3001 tcgttgcgca tgctcggcgc gtatggtttt ctgcgcgcgc tgtttgaagt gtttgaccgg
     3061 caccgcgtgg tgattgacgt ggttacgacg tcagaagtaa gcgtgtcgct ttcattggat
     3121 gaggctggcg cgttgccggc gatagtcgag gagttacaac aactggggac ggtcgggatc
     3181 gagaagggaa gagcgatcat ctgcgttgtt ggtgaaggct tacgcgggac ggcggggatc
     3241 gaggcgcgcg tgttcagcac gatcagtgat atcaatgtgt cgttgatctc gcagggagcg
     3301 tcgagtatca atttcacgtt tgtgattgaa gaagaacgtg ttaaggaagc ggtcacgaga
     3361 ttgcacgagg agttttttag ccacgaaaag gcacaaaaag cacaaaaaga agatctatag
     3421 atgaatgttt gtgaattaac gcgacagttg attgatattc catcggtgac gggtgaagag
     3481 ttggcggtgg gtgagtttct cgcacgacat ctcgaggagc ttgggtatcg tgttgagagg
     3541 caggaggtcg cgactgatcg tttcaatgta attgcgacga cggcagcgct gccgcacttt
     3601 gtgttttcga cacacatgga cacggtgcca ccgttcatcg ggtcgagtga ggatgaggag
     3661 tttatttatg gtcgtgggtc gtgcgacgcc aaaggaatta ttgccgcgca gatctttgca
     3721 gcgcagcgtc tgcgtgatgg cggtatgaac aatgtgggac tgctgttcac tgttgatgaa
     3781 gagatggcca gtctcggtgc gcaggtggcg aacaagcatc cgatcgccaa gtcgtgcaag
     3841 ttcctcatca atggggagcc gactgataat cgcttagcaa ccggcacgaa gggttctgtg
     3901 cgtgtgatcg ttacgactga agggcgcgcc gcgcattctg cgtaccccga agcgggcgag
     3961 tcggcgatcg agaaactact cgatatactt caggacattc gtgcgattca atggcctgga
     4021 gatagcttct tcggtacgac gacgtgcaac atcggcgtgc tcagcggcgg tacgcggcca
     4081 aacgtgatcg cagataaagc acgtgcggaa ttgcaaattc ggctggggat cgatatagag
     4141 cacatcaagc gtaagctcga agatgcggtg ggcacgcgcg ggcgacccga atacagctca
     4201 gcgcacaatc cggtgcgatt gttttctgtt ccggggtttg aagagtgcgt ggtgcgtttc
     4261 acgacagacg tgccgtattt gtctgagtgg ggcaaaccgt tattgcttgg accaggatcg
     4321 attttcgacg cgcacacgga tcacgagcgg atctcaaaac gtgaacttga acaagccgtc
     4381 gacttatatt tgagattgag taaacaatta attccatgaa actcgcttta ttcggatacg
     4441 gagcgatggg ccagttggtt gctgccggcg cgcgcaaggg cggcgatgag atcggtgtga
     4501 tcttaacgag taaagacgat ctgcccgtgg atcaactggc ggagaagttg agcggtcacg
     4561 acgtcgcgat cgatttctca atcggcgacg cagtgatcaa aaacatcgag gtatgcgcgc
     4621 tcgcacacgt gccgctcgtc gaaggcacta caggttggaa acaacacgag tcgactgcga
     4681 aacaactcat caccgaacac tcgggcgcga tggtctacgg tgcaaacttc tcgatcggcg
     4741 ttaatctttt ttaccgcatc accaaatacg cggccgcatt gttttctgct gtcgacggct
     4801 acgcaccgtt tattgaagaa gctcatcaca accgcaagcg cgatgcgccg tcaggcaccg
     4861 ccttgaaact gcgcgacttg atgtcagaac gtctcgggcc tgacatcccg acttcatcga
     4921 cgcgcgcagg ctacatcccc ggcacgcatc gagtcggttt cgattccgaa gccgatcaaa
     4981 tcttgttaac gcacacagcc cgctcgcgcc agggtttcgc aaacggcgcg ttgctggcag
     5041 cgcattggat cgtcggacga accggcgtct ttgaattctc agatgtgata gaggaaatca
     5101 tcaccacaaa aagcacataa agaattcctc ttttgcgctt tttgagcctt tttgtggcta
     5161 aaggagatct actatgacaa tacaactcga ctggcttcgc ggctgcgcga cggctctcgt
     5221 tacacccttc acggccagcg gcgcggttga cgagccacgt ttgcgcgact tgatcgaata
     5281 tcaaattacc ggcggcgttc gcatcctcgt tccgtgcggc acaaccggtg agagcgtgac
     5341 gatgactgac gaagaaaatc gtctcgtcat cagcaccgcg gtcgagctcg ctcgcggtcg
     5401 cgcgaaagtg atcgccggca ccggcagtaa ctcaaccgcc gtcacgattc aacgctcgca
     5461 aaacgcgcgc gctctgggcg tcgatggcgt gctgacagtc gcgccttatt acaacaagcc
     5521 aactcaggac ggactgtacg cgcacttccg cgcgatcgct gaagcagtca gcgatttgcc
     5581 ggtcgtgatc tacaacgttc ccggacgcac atcgtcgaac atctccgccg acaccaccct
     5641 gcggctcgca cgcgacgtcg agaacatcgt cgccgtgaaa gaagcctcgg gcaatctctc
     5701 gcagatcatg gagatcctgc gcagccggcc cgaaggtttt cgagtgatct ccggcgacga
     5761 ctcactcacg ctcgctttga tcgcacttgg cggcgaaggt ctgatctcgg tggcttcgaa
     5821 cgaggctccg gaaatgatgt cgcgtctcaa cgagctcgcc ctcgccggcc agtgggacga
     5881 agcacgcgcg ctgcactatc gtctgctgcc gttgatggaa ggcaacttca ttgaatcgag
     5941 tcctggtcca gtgaaggcag gcctcgctct gatgggaatg atcgaagaga acctgcgttt
     6001 gccgctcgtg ccggtgcagg aaaagacgcg caatcgcatg cgcgagattt tgaccgaaat
     6061 cgggttactg aaggaaacgt cgcatgctgt tgcgtgaaca gatcgaggcc ctggcggcgc
     6121 gcgcagacaa ccagttctcg tgcgcagaca gggccgtatt cgacgagctg aagcgcgccc
     6181 taaatcgcgg cgaagtgcgt gctgctgaaa aaaacagcga cggaaagtgg atcgtaaact
     6241 cgtgggtcaa gcagggaata ctcgtcggct ttcgcatggg cgcgatcgtt gacatgtcag
     6301 tgggcgcgag ctttaagttt ttcgacaaag acacttaccc cggtcgcccc acagcgatcg
     6361 aagacaacgt gcgcatcgtt cccggcggct cgacgatcag agacggtgtt tacatcgcgc
     6421 cgggtgtggt ttgtatgccg ccgatgtttg tgaacgcggg cgcctatgtc gacgagggca
     6481 cgatgataga cagtcacgcg ctcgttggct cgtgcgcgca gatcggcagg cgcgtacata
     6541 tctcagcagc ggcacaaatt ggcggcgtgc tcgagccggt cggcgccgtg cctgtgatca
     6601 tcgaaaacga cgtgctcgtc ggcggcaatt gcggggttta tgaaggaacg atcgtgcgcg
     6661 agcgtgcggt gctggcgtca ggcacgatcc tgaccgggtc gactccggtt tatgatcttg
     6721 tgcgcgggga gatctaccag cgcgcttccg atggaccact cgagattccg gcaggcgcgg
     6781 tggttgttcc cggtgcgcgc gcagtgacga ccgagcgtgg acgcgagtgg ggactctcgc
     6841 tttacgccgc ggtgatcgtg aagtatcgcg atgagaagac cgacacggcg gtgcgcttag
     6901 aggattattt gcgttgaata aatttgcacc accgaaacgc ttgcagggaa tcgagaagag
     6961 cgttattcgc caggtcttcg atcgcgcgca gcccggcagt ctcaatctcg gattgggtga
     7021 acccgatctg cccacgccgg acgtgattcg tcgcgctgca gtcgcagcga ttgtcgaaga
     7081 gcagaacggt tacacgtcac acgctggact tccggcgctc cgtgagaaag tcgcgaatga
     7141 gtatccgtat ctcgaagggc gtcccgagcg cgtcgtgatc acggcaggtt cgcaggaagc
     7201 attgtatctc gcgctgctga cgctcgtcga tgaaggcgat gaggtgttat tgccgaatcc
     7261 cgggtttgtt gcgtacccga cgatcgtgcg catggcggga ggcacgtcgc gcttttatcg
     7321 tttgccgcgc gagcatggtt tcgcttttga tatcgatgag tttcgtcgca cgctaacgcc
     7381 gcagacaaag gtggttgttt gtatcagtcc atccaacccg actggccgga tcttaacaaa
     7441 cgaagatttg catgctattg ccaatacgtt gcgcgatcac ggtgcttacc tgatcagcga
     7501 tgagatatat cgcgatcttt actacaccga tgagcggccc ggatcgttgt cgtcgttttc
     7561 cgatcgcacg atcgtgatca gtggactatc gaagtcgatg agcatgaccg gatggcgact
     7621 cggttgggtg tgtggtgctg aagaattgat caaggcgatg ctggtgctgc acggctacgt
     7681 gacgacgtgt gcgtcgacgg tgtcgcagaa ggcgtcgttg gtggcgtgga gtgacgaagc
     7741 ggaagaggcg cgtgccggtt ttcgagacac gtttcgagcg cgtcgggatc atttgttggg
     7801 attggttgag agtgagttga gattaaaagc agtgacacct gatggtgcgt tttatacgat
     7861 gctcgatgtg agcgagtatg gatcgtcgat gaaggtggcg gaggcgttgc tcgaagagaa
     7921 ggtgatcacc gtgcccggcg cggcgtttgg gagtgaaggc gaggggtttt tgcgtgtttc
     7981 gttttgtgct gataaggaga tgttggacga aggtgttcag cgaataaaga aagccatcca
     8041 cagattacac agaaaaaatc tgtgaaatct gtggttaaaa aaagggttta tgaaaaaagg
     8101 gtttatgaaa aagtatcgtg taggcatttt aggtgcgacg ggtatggtgg gccagcggtt
     8161 tattcaactg ctggagcatc atccgcagtt tgaaatcacc gcggtcgctg cttccgatcg
     8221 ctcacaaggc aaaacttatg gcgaggcatg cacgtggcgg ctcaatggtg aaatgcccgt
     8281 cgccgtgcgc tcgttaccgg tgcaaccgcc gcaacctcca ctcgactgcg atctggtgtt
     8341 ctcgagtctg cctggtgata tcgcacgcga gacagaaggc agttttgcaa atgccggcta
     8401 tccggtcatc agcaactcat ccgcctttcg catggatgaa gatgtgccgt tactaattcc
     8461 cgaagtaaat caccagcatc tcggcttgct cgcacgccag cgacggacca atacatcagg
     8521 cggcttcatc gtcaccaacc cgaattgctc gacgatcatg atcgccctcg cgcttgctcc
     8581 actccatgcg acctttggta tcacgtcaac ggtggcgaca acaatgcagg cactctcggg
     8641 cgccggatac ccgggcgtcg cttcgctcga catcatcgac aacgtcctgc cgcacatcgg
     8701 cggcgaggaa gagaagatcg aaaccgagac gacgagaatt ctcgggcgtt ttatgaacga
     8761 tgcaatcgaa cctgcgtcga tggcggtgag cgcgcagtgt catcgtgtga atgttgctga
     8821 cggtcacatg gccgcggtgc gcgtgaactt cacacgcaaa cccgcgctgc ccgacctgcg
     8881 cgaagcattg acctcgtttc gatcgttgcc gcaggagctt gggcttcatt ccgcgccggc
     8941 aaatccgatc gtcgtacgtg atgaacagga tcgtccgcag ccgaaactcg atcgtgatgc
     9001 cggcaatggc atgacgatca cggttggaag attgaaggaa gatacggtgc ttgattatag
     9061 gtttgtggtg ttgagtcaca atacgattcg cggcgcagcg ggcgcggcga tactcaatgc
     9121 ggagttgttg attgctttag gacagttgta gacaggataa acacgattct tcatgatgaa
     9181 caagattagg tttctaatac ctctactgtt cctattcagc atttcagttc aggcgcaaac
     9241 aaatctcgac gatcaagtga agcccatcgt cgcgtcgttc aaaggcaagg tgagtctctt
     9301 cgccaggaac tcgacacagg ggagacgtac ggattgaatc ccgacgagcg tgtgcgcacg
     9361 gcgagcacga tcaagattgc cgtgatgatc gaggcattcg cgcgcgtcgc ggaaggaaaa
     9421 gcgaagtgga ctgacgaggt catactcaca aaagaaaaga aagtctccgg ttcgggtatt
     9481 ctcaatgagc tatcagacgg tctgaagcta acgctacgcg acgccgtcaa cctgatgatg
     9541 atgatcagtg acaacaccgc gacaaacctc gtgctcgacg tgttgacgac cgacgctgtg
     9601 aacgcgcgca tggagtcgct cggtttcaag cagataaaga tcatgcggaa ggtaggcagc
     9661 ggcggcgaaa gcactgccgg caaagatccg gagaacaaaa agtacggact cggcatggcg
     9721 acgccgcgcg agatggtgct cgtaatggaa aaactcgagc gcggtgaagt gatcagtccc
     9781 gctgcatcga aagaaatgat cgacttgatg aagc
//
LOCUS       DASSFQ010000035         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1210735, whole genome shotgun sequence.
ACCESSION   DASSFQ010000035 DASSFQ010000000
VERSION     DASSFQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210735"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1409)
                     /locus_tag="VFH96_01250"
     CDS             complement(78..1409)
                     /locus_tag="VFH96_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET6972615.1"
                     /translation="MALRERLIGNNNGGTGPVADGTGQHSFQEMKSRLHRTLINRMDL
                     TKLQTLTPEQVHAEVSRLAEAVLAQEAMPLSVSERERLVSDVQHELFGLGPLEPLLKD
                     PTISDILVNKHDTIYIERRGKIERTNVTFKDDEHLMRVIERIVSSVGRRIDESSPMVD
                     ARLQDGSRVNAIIPPLAIDGPVVSIRRFGADPLKMAMLIENKALTKDIADMLQMVVHA
                     RLNVLISGGTGAGKTTLLNALSAYIPENERIVTIEDSAELQLQQPHTVRLETRPPNIE
                     GRGEVTQRDLVRNALRMRPDRIVIGEVRGGEAIDMLQAMNTGHDGSLTTIHANTPRDA
                     LSRLETMIQMTGMRLSDRAMRQQVASAINIVLQVARMSDGSRRVTSISEITGMEGETI
                     TMQEIFQYERTGVDSQGTVLGRFRPTGIRPRFAERLKSCGLQLPRVFFEEM"
     gene            complement(1440..2669)
                     /locus_tag="VFH96_01255"
     CDS             complement(1440..2669)
                     /locus_tag="VFH96_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6972616.1"
                     /translation="MSEPISVVVLSTGLENFKDIRKALTAEPRVKLLAGGNDTEQLYE
                     EIVRLKPAAAIIALHGANADQAVKFIEKLNSECPETALISAAQDASPDLILRSLRAGA
                     REFLRIPISTDELRTVLDRISEFCTTKVEAPKKKGRMVAVFSSKGGCGTSFIATNLAA
                     ATNSKTILVDLNLQAGDLPLFLGLEAKYSISDMVEKRQRLDETLISSLVTPHSQHLSL
                     LAAPREADSADEIEPQHIFEVLEKLREHYDYVVLDPQHTFDSITLAALDQSDEIVLIL
                     TLDIPAIRSTQRALEIFDRLGYPRKKVRIVVNRWSKQIDLDLRQVEKFLGEPVIGFVP
                     SDYQTAVGSINLGTPLIQAEPTSKIALEIRRIAQQISLGLAPIDEHKQRRPFWAGLLK
                     KQAAQPNLKLQTSMEKV"
     gene            complement(2868..3305)
                     /locus_tag="VFH96_01260"
     CDS             complement(2868..3305)
                     /locus_tag="VFH96_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HET6972617.1"
                     /translation="MLRKKNNERGSTLVEFAIGVTVFVTAMFAVLEFGRALWVHNALS
                     DAARRGARYAVLHSSGSAAQVKNIVVYGNAAGTGQPMLNDLTTANVNVVYNNFGLNDG
                     TVSVSITGYQFQLVIPIIGRTITMPSYTTTLGAESAGFLPANV"
BASE COUNT          680 a          911 c          991 g          805 t
ORIGIN      
        1 ccgtgcgtag caagcaggta ggcgcccagc accaggaaca acgcaaacac gaatacgaga
       61 aacgatatga tcatcgatta catctcctcg aagaagacgc gcggcaactg caaaccgcag
      121 gatttcaacc gttcggcgaa ccgcgggcga attccggtcg ggcggaagcg tccgagcact
      181 gtgccctgcg aatcgacgcc ggttctctcg tattggaaaa tctcctgcat ggtgatcgtc
      241 tcgccttcca tgccggtgat ttcggaaatc gaggtgacgc ggcgcgaacc gtcagacatg
      301 cgtgccactt gcagcacgat gttgattgcg gacgccacct gctgtcgcat cgcgcgatcg
      361 gacagacgca taccggtcat ctggatcatc gtctcgagtc gtgacagcgc gtcgcgtggt
      421 gtgttggcgt gaatcgtggt caagctgccg tcgtggccgg tgttcatggc ttgcagcatg
      481 tcgatcgctt caccgccgcg cacctcgccg atcacgatgc gatcgggacg catacgaagc
      541 gcgttgcgca cgaggtctcg ctgcgtaact tcgccgcgac cttcgatgtt cggtggccga
      601 gtttcgagtc tgactgtgtg cggctgctga agctgaagtt ctgctgagtc ttcgatggta
      661 acgatgcgct cgttctcagg aatgtatgcc gacagcgcgt tgagcaaggt cgttttaccg
      721 gcgccggtgc cccccgaaat caaaacgttg aggcgcgcgt gaacgaccat ttgcagcatg
      781 tcggcgatgt ctttggtcag cgctttgttt tcaatcaaca tcgccatttt cagcggatcg
      841 gcgccaaagc ggcggattga aactactggt ccatcgatcg ccagcggcgg aatgatcgcg
      901 ttgacgcgcg aaccgtcctg aagtcgtgcg tctaccatcg gcgacgactc gtcgatgcgg
      961 cgaccgactg acgaaacgat gcgctcgatc acgcgcatca ggtgctcgtc gtctttgaag
     1021 gttacgttcg tgcgttcgat ttttccgcgc cgttcgatgt agatcgtgtc gtgcttgttg
     1081 acgagtatgt cggagatggt cgggtctttg agcaacggct cgagtggtcc aagtccgaag
     1141 agctcgtgct gcacgtcgct gacgagtcgc tcgcgctctg aaacggaaag cggcatcgct
     1201 tcctgggcca gaacggcttc ggccaaacgc gaaacttccg catggacctg ttccggagtc
     1261 agcgtctgga gcttggtcag atccatccgg ttgattaacg tccggtggag acgcgacttc
     1321 atttcctgaa atgaatgctg tccagtgccg tccgcaactg gcccggtacc gccgttattg
     1381 tttccgatta gtctttcacg aagtgccatt gtttttgttc atctgcgggg ctgccgctgt
     1441 tagacctttt ccatcgaggt ttgcagtttt aagttcggtt gggctgcctg ctttttgagt
     1501 agaccagccc agaacggacg gcgttgtttg tgttcgtcga tcggcgcgag accgagcgat
     1561 atctgctgcg cgatacggcg aatctcgagc gcgatctttg aagtcggctc cgcttgtatg
     1621 agcggcgtcc cgagattgat cgatccgact gcggtctggt agtcgctggg tacaaagcca
     1681 attaccggct cgccgagaaa cttctcaacc tgcctgagat cgagatcgat ctgcttgctc
     1741 cagcgattca cgacgatgcg cactttctta cgcgggtaac ccaggcgatc gaagatctca
     1801 agcgctctct gcgtgctgcg aatcgcggga atatcgagtg tcaggatcag gacgatctcg
     1861 tctgactggt ccaatgcggc gagcgtgatc gagtcgaacg tatgctgcgg atcgagcacc
     1921 acgtagtcgt aatgctcgcg cagtttctcc agcacttcga agatgtgctg cggctcgatc
     1981 tcgtcagcgg aatcggcttc gcgtggcgcc gccaaaagag acaagtgctg cgagtgcggc
     2041 gtcaccagac tgctgatgag tgtttcatcg agacgctggc gtttctcgac catgtcagag
     2101 atcgaatact tcgcttcgag tcccaaaaac agcggcagat ccccggcctg aagatttaga
     2161 tcgacgagaa tcgtttttga attcgtggct gctgcgagat tggtcgcgat gaaagaagtg
     2221 ccgcagccgc ctttgcttga aaagacggcg accatacgac cttttttctt cggtgcctca
     2281 accttcgtag tacaaaactc tgaaatccga tcgagcacgg tgcgcagctc gtctgtgctg
     2341 attggaatgc gcagaaactc acgcgcaccg gcgcgcaagc tgcgcagtat cagatcggga
     2401 gacgcatcct gcgcggctga aatgagggcg gtttccggac attcggagtt aagtttttca
     2461 ataaatttaa ctgcctgatc ggcattggct ccatggagcg cgatgatcgc ggccgcaggc
     2521 ttaaggcgaa cgatctcttc gtaaagctgt tctgtatcgt taccgccggc gagcagtttg
     2581 acgcggggct cagcagtcag ggctttgcgg atgtctttga agttttcgag tccggtactg
     2641 aggactacta ctgaaattgg ttcgctcatt ttttcctcgt gttaattcga atcccactac
     2701 cggcggctgt cgtgaattga tctagtgaca gagtgcctgg gggttacggt aggtccatcg
     2761 caatgttgca aggccgggtc aaccggagcg atgttcgaac ggctggcgaa atactggcgc
     2821 cgtccggcag tggattcgaa acttgagact ggcattttcc cattgttcta aacgttggcc
     2881 ggtaaaaagc cggcgctttc ggcgccgagc gtcgtggtgt agctcggcat cgtgatggtc
     2941 ctgccaatga tgggaatcac aagctggaat tgataaccag ttatgcttac tgaaaccgtc
     3001 ccatcgttga gaccaaaatt gttgtagacg acgttcacgt tcgcggtggt caggtcgttc
     3061 agcatcggtt gacctgttcc cgcggcatta ccgtagacga cgatgttctt gacctgagcg
     3121 gcgctgcccg aggaatgcag gactgcataa cgcgcccctc gacgggcggc gtcagacaaa
     3181 gcgttgtgga cccacaacgc tcgcccgaat tccaaaaccg caaacatcgc cgtcacaaac
     3241 accgtgactc caattgcaaa ctcaaccaga gttgagcctc tttcgttgtt ctttttgcgt
     3301 agcattgttc tttgcctctc agattgcagg cgttgttagc aggtaacgca tcgttacgct
     3361 cggcttgacg tcgacgttgg tcgagag
//
LOCUS       DASSFQ010000036         7198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1505679, whole genome shotgun sequence.
ACCESSION   DASSFQ010000036 DASSFQ010000000
VERSION     DASSFQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505679"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFH96_01265"
     CDS             <1..774
                     /locus_tag="VFH96_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HET6972618.1"
                     /translation="HKELDELPQADNRDERLARIVWTLQAIETRVAERMNPGGSPAEN
                     KQWAKTRLESILARPEYAADTEGSNALFRLIRDFFEWLAKFLPKPRAGNGSRVNLITR
                     IAQVVVIVLAVAVLLYVLKTLYVRLSGRSRKPKASNKREARIVLGERLEPEATATDLL
                     SEAEALARQGDLRAAIRKAYIALLVELGDRKMISLAHHKTNRDYLNSLRSVPQLHSRM
                     RGLTDSFERHWYGFASATPNDWQDFRAGYIAALQTETSN"
     gene            786..2120
                     /locus_tag="VFH96_01270"
     CDS             786..2120
                     /locus_tag="VFH96_01270"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HET6972619.1"
                     /translation="MRQRLTIILTFAVIIGLLALVSSIRYVRKEKEQDFEFAPNRSTY
                     HSGPTGVRAFFDFLHESGYKVIRWRDQPQKLLTESGKDIKTFVVVGQTKVHFTDAESE
                     ALTKWIAAGGCYVIIDRRPLSEEKAKTKTSWYIIAKQLEFPALEVNPANLEQMTGDVV
                     ALQPVQPTVFTHNIQSVLPSRFASRMTVVPPAEVEPQDDGAPPFAETAGKSPAPVIHI
                     ADDKGALLIDYTYGSGRVVLLSDPYIVSNSGIKLNDNLQLALNILTAREGLIAFDEFH
                     QGKGPIENSFAGYFAGTPVLLIAGQVGLIVLLVLWTKGKRFGRPLPLRQVDRRSSLEF
                     VASMAELQQRARAFDLAIENIYTRTRRVLARHAGVDYNSSRSEIASRIALRGTIDANR
                     LETLMRECEEVINGRPCTWRESIDLVRRLREVERDLGLRMRQREMRQAAENI"
     gene            2144..3097
                     /locus_tag="VFH96_01275"
     CDS             2144..3097
                     /locus_tag="VFH96_01275"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6972620.1"
                     /translation="MPETVASTVEHIRTELQKVIVGQDDAIEQILIALLAEGHALIEG
                     VPGTAKTLTVKTIARIINADFGRIQFTPDLMPSDITGTNVFNISNSTFTLRCGPIFTD
                     ILLADEINRTPPKTQAALLEAMEERQVTIDGEAHKLSPIFTVLATENPIEYEGTYPLP
                     EAQLDRFQLKILLTYPSQEHELEVVSRWNDGFNSRRLDQVDIRPLQDPDAILHCRAEI
                     ARARMEPGVQRYAVDLVRRTRAHPFIHYGASPRASVALLLCSKALAAIRGREFVTPDD
                     VRDVALPVLRHRLTLRAEAELDGVTTDSVITDILKTAEVPR"
     gene            3197..4516
                     /locus_tag="VFH96_01280"
     CDS             3197..4516
                     /locus_tag="VFH96_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6972621.1"
                     /translation="MRFVFTKLFYFLLALALIPLLLSWERPWLRWIALLYNVLLVVGV
                     VIESRFCRLPKGLMISREFGSRFAMGAETEVHIHVQNASSKPVSLMLKDEYPPQMSLN
                     GLREGQVDVDAQSTATLVYAVKPPKRGRFEFGQTALRFKSRSKLIWCQTKVVEPVTVK
                     VYPNMRRAREAELKALGARSVVSSHRKTSWRGEGREFESMRDYVRGDELRHISWTATA
                     RRGKLTTRQYQIERDQTILIALDAGRLMTARIEQETKLDSAVHATLALFSAAARAGDN
                     AGLVVFGRGIKSYLPPGRGRDHIESALEALYAVEPEMIEPSYPHAFEFIAGSSKRRSL
                     VVLLTDLVDEEGSKELLNSLHILRPRHLPLVVTIADRDLKAVVRQIPSSIKDVFTQSV
                     SEEIIHHREAALRMVESIGGLALDVTAAALAPALLETYLRVKERGLL"
     gene            complement(4556..5539)
                     /locus_tag="VFH96_01285"
     CDS             complement(4556..5539)
                     /locus_tag="VFH96_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET6972622.1"
                     /translation="MPQDRFVRQRKNAWQRLEDLLKLLDASSLKRLGREEVRELARIY
                     RRTASDLAIARAESRDPRLVNYLNSLVIRAHGRIYRADAQGGKRIRLFFTRELPRTFR
                     RTWRYTFLAFAVFALCSIVSFVGTYRDPDFSEVVGVSPQFRELSIETHTRWWEELNEA
                     NQAGASMIMTNNIMVTIYTFAYGATFGIGTLLYLAYNGLMIASVVALVYRAGYGNELV
                     TFMVGHGVVELSCIFIAGGAGLLIGSAMLMPGDLTRADALKTRGMEAVRLMIGVALFL
                     VVAGTIEGFISPAPIDPRFKYGIGAITGVAMFSYLLLVGRDSGQDLQNFQN"
     gene            5599..6486
                     /gene="ftcD"
                     /locus_tag="VFH96_01290"
     CDS             5599..6486
                     /gene="ftcD"
                     /locus_tag="VFH96_01290"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET6972623.1"
                     /translation="MSLVECVPNFSEGRRPDVIQQIADSITSVESACVLDTHIDPDHN
                     RSVITFVAPPETVVAAAVNAVRRASALIDMRTHQGEHPRLGATDVLPFVPVRGVTMDD
                     CVRIAHEAGAIIARDLSIPVYFYERAALREERVNLEDVRRGALELLREQISINPDRAP
                     DAGSAQVHESAGAIAVGARPFLIAFNVILRSNDIAAARQIARTIRARNGGLPFVKALG
                     FRLHTRGLVQVSMNLVNYEVTGMTAAYNAIRRESDRLGIEIDGTEIVGVVPRAAMDPT
                     APFFSEAKILEHQIRVICG"
     gene            complement(6483..>7198)
                     /locus_tag="VFH96_01295"
     CDS             complement(6483..>7198)
                     /locus_tag="VFH96_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972624.1"
                     /translation="LSPQEGEKALDLARDASTIRYRELYGFTHGDPKHVYRAELGRGV
                     ELFIMSLPQEKRLPLRSYHAVMIYKNGVAIGYFEGLSLFERMESGFNLYYTFRDGETA
                     WLYARVLNVMHHFAGVTAFSLDPYQIGFENEEGIESGAFWFYRKLGFRPTDPEILKVV
                     LKEEEKIRTRKGYRTSAATLRKLARSPMIFELDEKRIGDWDRFQIRKIGFRVQRGLSA
                     EEKQGLDERLRSVIIKLGS"
BASE COUNT         1638 a         2059 c         1965 g         1536 t
ORIGIN      
        1 cacaaggaac tcgacgagct cccgcaagcg gataatcgtg acgaaagatt ggcacgcata
       61 gtctggactc ttcaggcgat cgaaacgaga gtggctgaac gtatgaaccc cggcggcagt
      121 ccggcggaga acaagcaatg ggccaagaca cggttggaga gtattctcgc gcgaccggaa
      181 tacgctgccg acacagaagg ctcaaacgct ctcttccgat tgatccggga ctttttcgaa
      241 tggctggcga agtttttgcc gaagccgcgt gccggcaacg gaagcagggt caacttgatc
      301 acgcggatcg cgcaggtcgt tgtcatcgtc cttgctgtag cggtgctgct ttacgttctc
      361 aaaactctgt acgtgcggct ttcaggacgc tcgcgaaaac ccaaagcgag caacaaacgc
      421 gaggcgcgca ttgtcttagg cgaacgactc gagcccgaag cgacagcgac ggatctgctt
      481 tccgaagcgg aagcgcttgc cagacagggc gacctgcgcg ctgcgattcg caaggcttac
      541 atcgccttgc tggttgaatt aggcgaccgc aaaatgatca gtctcgcgca tcacaaaact
      601 aatcgtgatt atttgaactc gcttcgcagc gttccccagc ttcattcgcg tatgcgcggg
      661 ctcaccgata gtttcgaacg tcactggtac ggtttcgcga gtgcgactcc aaacgattgg
      721 caggactttc gcgccggtta catagcagcc ttacaaactg aaacaagcaa ctgacacaac
      781 cagcgatgcg ccagcgactc accatcattc tcactttcgc agtcatcatc ggcttgctgg
      841 ccctcgtcag cagcatcagg tatgtaagaa aagaaaaaga acaggatttt gagttcgctc
      901 caaatcgttc cacctatcac tccggaccaa caggggtgcg cgcatttttc gacttccttc
      961 acgagtcagg ctacaaggtg attcgctggc gcgaccaacc gcaaaaactt ctgactgagt
     1021 ccggcaaaga catcaaaacg ttcgtagtcg tgggccagac aaaagtgcac ttcactgacg
     1081 cggagtcgga ggcactaaca aagtggatcg ccgcgggagg ttgttacgtc attatcgatc
     1141 gccgtccgct cagtgaagag aaagcgaaga ccaaaaccag ttggtacata atcgccaaac
     1201 agcttgagtt tccggcatta gaagtaaacc cggctaatct cgaacagatg accggtgacg
     1261 tagttgcact gcaacccgtg cagccgacag ttttcacaca caacattcaa tcagtcttgc
     1321 cttctcgttt cgcatcacga atgacggtgg tgcctccggc ggaagttgag ccgcaagacg
     1381 acggtgctcc gccttttgca gaaacagcag ggaagtcgcc ggcgcccgtc atacacattg
     1441 ctgacgataa aggagcgttg ctgatcgatt acacttacgg ctcgggcagg gtcgtgcttc
     1501 tcagcgatcc ttatattgtt tcgaacagcg gcatcaagct aaacgacaac cttcaactcg
     1561 cactcaacat tctcaccgca cgcgaagggc tgattgcttt cgacgagttt catcagggaa
     1621 aaggcccgat tgaaaacagt ttcgccggtt atttcgccgg cacgccggtg cttttaattg
     1681 ccggacaagt gggattgatc gtgttgttgg tgctttggac aaaaggaaaa cgctttggac
     1741 gcccactgcc gttgcggcaa gtcgatcgtc gttcgagtct ggagtttgtg gcgtcgatgg
     1801 ccgagctgca gcaacgcgct cgtgccttcg atctcgcgat cgaaaacatc tacacgcgca
     1861 cgcgccgcgt gcttgcccgg catgcgggtg ttgattacaa cagctcgcga tctgaaatcg
     1921 catcacgcat cgctttgcga gggacgatcg atgcgaacag attagaaacg ttgatgcgcg
     1981 agtgtgaaga agtcatcaat ggccggccgt gtacctggcg ggaatcgatc gacctggtgc
     2041 ggcgtttgcg cgaagttgaa agagatctcg gcttgcgtat gcggcaaaga gaaatgagac
     2101 aagcagcaga aaacatttaa tcttgtgcta taactgataa ccgatgcctg agaccgtcgc
     2161 ctccactgtc gaacacattc gcactgaact gcagaaagta atcgtcggac aagacgacgc
     2221 gatcgaacag atcttgatcg cgttgctggc cgagggacac gcgctcatcg agggcgtgcc
     2281 cggcacggcg aaaacactga cagtcaaaac cattgcgcga atcatcaacg ctgatttcgg
     2341 ccgtattcaa ttcacgcctg acctgatgcc ttcggacatc accggcacga acgtcttcaa
     2401 catctccaac tcgacgttca ctttgcgttg tggaccaata ttcactgaca tcctgctggc
     2461 cgacgagatc aaccgcacgc cgccaaagac gcaagccgcg ctgttggaag cgatggaaga
     2521 acgccaggtg acgattgacg gtgaggcgca caagttgtca ccgatcttta cggtcctcgc
     2581 gacggaaaac ccgatcgagt atgaaggaac gtatccactg cccgaggccc agctcgatcg
     2641 tttccaacta aaaatattat tgacttatcc cagccaggag cacgagctcg aagtagtgtc
     2701 gcgttggaac gacgggttca attcgagacg cctcgatcag gtcgacatcc gaccgctcca
     2761 ggatccggat gcgattcttc attgccgcgc ggaaatcgcg cgcgcgcgca tggaaccggg
     2821 cgttcagcgt tacgcagtgg atctcgtgcg gcgcacgcgc gcgcatccgt tcattcatta
     2881 cggtgcgagc ccgcgcgcgt cagtcgcctt gttgctttgc tcgaaagcgc tggcggcgat
     2941 tcgtggtcgt gagttcgtaa cacctgacga cgtgcgcgac gtcgcgcttc ccgtccttcg
     3001 tcatcgcctg acactgcgag ccgaagccga gctggacggc gtcaccacgg attccgtaat
     3061 caccgacatc ttaaagactg ccgaagtccc aagataggcc acaaaaggca caaaatgaag
     3121 aatatggggt cgcgcctgaa cttttctttt atgtgctttt tgtgcctttt cgtggctaac
     3181 tctttttgag gctaccgtgc gttttgtttt cacgaaactc ttctacttcc tgctcgcgct
     3241 cgctttaatc ccgctactgt tatcgtggga gcggccgtgg ctgcgctgga tcgcactcct
     3301 ctacaacgtt ttgcttgtcg ttggtgtggt gattgagtcg aggttctgcc ggcttccgaa
     3361 aggcctgatg atctcgcgcg agtttggcag ccgcttcgca atgggcgccg aaaccgaagt
     3421 ccacattcac gtccaaaacg cctccagtaa accagtatcg ttgatgctga aagacgaata
     3481 cccgccgcag atgagtttga acggtctgcg tgagggccag gttgacgtcg atgcgcagag
     3541 cacggcgacg ttggtttacg cagtcaaacc accgaagcgc ggtcgttttg agtttggaca
     3601 aactgcgctg cgcttcaaat cgagatccaa actgatctgg tgtcaaacaa aagtggtcga
     3661 gccggtgacg gtgaaggttt atcccaacat gcgtcgcgct cgggaagcag agttgaaagc
     3721 actcggcgcg cgctcggttg tttcctcaca tcgcaaaact tcgtggcgcg gtgagggtcg
     3781 cgagtttgaa tcgatgcgcg attacgtgcg cggcgacgag ttgcgtcaca tctcgtggac
     3841 cgcgaccgcg cgacgcggaa aactcacgac gcgccagtat cagatcgaac gcgatcaaac
     3901 gattctgatc gcgctcgatg ccggtcgttt gatgacggcg cggatcgagc aggagacaaa
     3961 actcgattcc gcggtacacg ccacgctcgc gttgttttca gcggcggcac gcgcgggcga
     4021 caacgccggg ctcgtcgtct ttggacgcgg catcaagagc tacctgccgc cgggtcgcgg
     4081 gcgcgatcac atcgaatccg cactcgaagc gctgtatgcg gtcgaaccgg agatgatcga
     4141 gccgtcgtac ccgcacgcgt ttgagtttat tgcggggagc agcaagcggc gatcgctcgt
     4201 cgtgttgttg acagatctag tggatgaaga gggatcgaag gagttgctca attcgttgca
     4261 cattttgcgg ccgcgtcatt tgccgctcgt ggtgacgatc gccgatcgag atttgaaggc
     4321 ggtggtgcgg cagattcctt catcgatcaa ggatgtgttt acgcagtctg tctcggagga
     4381 gattattcat caccgagaag cggcgctgcg gatggttgag tcgattggtg gactggcgct
     4441 cgacgtcact gcggctgcgt tggcgccggc attgctcgag acctatttgc gggtcaaaga
     4501 gcgaggtttg ttgtagacat gatttacaag atttgcatga tttacaggaa tcttgttaat
     4561 tctgaaaatt ttgtaaatcc tgtccactgt ctcttccgac aagcagcaaa tacgaaaaca
     4621 tcgccacacc cgtaatcgcg ccaatcccat atttgaaccg cggatcgatc ggcgcgggcg
     4681 agatgaaacc ctcaatcgtt cctgccacca caagaaaaag cgctacgcca atcatcaacc
     4741 gcaccgcttc catgccgcgc gtcttcagcg catcggcgcg cgtcaaatcg ccgggcatga
     4801 gcatcgcact accgatcaac aaacccgcgc cgccggcgat gaagatacac gacagctcaa
     4861 ccacgccgtg gcccaccatg aaagtcacga gctcatttcc atagcccgcg cgataaacca
     4921 gcgctactac tgaagcgatc atgaggccgt tgtacgcgag atacaaaagc gtgccgatac
     4981 cgaaggtcgc gccatacgca aaggtgtaga tcgtgaccat gatgttattg gtcatgatca
     5041 tactcgcgcc tgcctggttc gcctcgttga gttcttccca ccagcgcgtg tgcgtttcga
     5101 tgctcagctc gcgaaattgc ggcgagacgc ccaccacctc tgaaaagtcg ggatcgcgat
     5161 acgtgcccac gaagctaacg atcgaacaga gcgcgaacac ggcaaacgcc aggaacgtgt
     5221 agcgccacgt gcgacgaaac gttcgcggca gctcgcgcgt gaagaacagg cgaatgcgtt
     5281 tgccgccctg tgcgtcagca cgataaatgc gaccatgcgc gcgaatgacg agactattca
     5341 ggtaattgac caaacgcgga tcgcgcgatt cggcccgcgc gatggctaga tctgaggccg
     5401 tgcgacgata gatgcgggcc agctcgcgga cctcttctcg tcccaggcgc ttcagcgatg
     5461 aggcatcaag cagcttgagc aggtcctcga gccgttgcca tgcgtttttg cgttgtctga
     5521 cgaatcggtc ctgaggcaca acaaacaaga tacaagcatc atgtgaatcc tgtaaatcct
     5581 gttatcctgt ttcagtatat gtctctggtg gaatgcgtcc ccaacttcag cgaaggccgg
     5641 cgacctgacg tgattcagca gatcgctgac tccatcacct ccgtcgaatc agcttgtgtg
     5701 ctcgacacgc acatcgatcc cgatcacaac cgctcagtca tcactttcgt tgcgccaccg
     5761 gaaacggttg tcgcggctgc agtcaacgcc gtcaggcgag cttcagcgct catcgacatg
     5821 cgcacacatc agggcgagca cccgcgactc ggcgcgaccg acgtgttgcc gtttgttccc
     5881 gtgcgcggcg tgacgatgga cgactgcgtt cggatcgcgc acgaagccgg tgcgatcatc
     5941 gcgcgcgatc tctcgattcc agtttacttt tacgaacgcg ccgctttgcg cgaggaacga
     6001 gtgaatctcg aagacgtgcg tcgcggcgca ctggaattac tgcgcgaaca aatatcaatt
     6061 aatcccgatc gcgctcctga cgccggttcc gcccaggtac acgaatcggc aggcgcgatc
     6121 gcagtcggtg cgcgaccatt cctgattgcc ttcaacgtga tcctgcgcag caacgacatt
     6181 gccgctgcac gccagatcgc acgcacgatc agagcgcgca acggtggttt gccgttcgtg
     6241 aaagccctcg gctttcgtct gcacacgcgc ggcctggtcc aagtctcaat gaatttagtt
     6301 aattatgagg taacaggaat gaccgcggct tataacgcaa tccgccgcga atcggatcgc
     6361 ctcggcatcg agattgacgg cacagaaatc gtcggcgtcg tccctcgcgc cgctatggat
     6421 cccaccgctc catttttctc cgaagccaag atcctcgaac accagatccg tgtaatctgt
     6481 ggctaagatc caagcttaat aatcacgctt cgcaacctct catcaagccc ctgtttctct
     6541 tccgcactca acccacgctg taccctgaac ccgatcttgc ggatctgaaa acgatcccaa
     6601 tcaccaatcc gtttctcatc gagctcaaag atcatcggac tgcgcgccag cttccgcagc
     6661 gtcgccgccg aagtccggta tcccttgcgc gtccggatct tctcttcttc cttcagcaca
     6721 acttttaaga tctctggatc agttggtcga aagccgagct tgcgataaaa ccagaacgcg
     6781 cccgattcaa tgccttcctc gttttcaaaa ccgatttgat acgggtcgag cgagaacgcg
     6841 gtgacgccgg cgaagtgatg catcacgttc agcacgcgcg catacagcca cgctgtttca
     6901 ccatcgcgaa acgtgtaata caaattgaat ccgctttcca tgcgctcgaa cagcgagagc
     6961 ccttcgaagt agccgatcgc aacgccgttc ttgtagatca tcacggcgtg atagctgcgc
     7021 aacggcagac gcttttcctg cggcaagctc atgatgaaga gctcgacacc acggccaagc
     7081 tcggctcgat agacgtgttt cggatcgccg tgcgtgaaac cgtaaagctc gcgataacgg
     7141 atcgtcgagg cgtcgcgagc caggtcgagc gctttctcac cttcttgtgg cgacaact
//
LOCUS       DASSFQ010000037        14537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1955932, whole genome shotgun sequence.
ACCESSION   DASSFQ010000037 DASSFQ010000000
VERSION     DASSFQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14537
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955932"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFH96_01300"
     CDS             <1..531
                     /locus_tag="VFH96_01300"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972625.1"
                     /translation="TVMLTAYPEGAASLIDEVAESEMQAIIELISRVRNIRSEMNIKP
                     GERIPIVVAAPDESLRKVFSAAREQISRLVRASEVAVTERLEAPKASARAVLSGGAEL
                     AVPLEGLIDFEQERRRLQREQEKLQAEAAKLEAQLSNPNFVERAPVERVNEVRDRIAA
                     IAQQSSQLQQTVENLQ"
     gene            528..1382
                     /gene="nadC"
                     /locus_tag="VFH96_01305"
     CDS             528..1382
                     /gene="nadC"
                     /locus_tag="VFH96_01305"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET6972626.1"
                     /translation="MNWIDEGALFSQISLFLREDLGRGDITTQSIIARNTRARARFVA
                     GENMIVAGLEAAEEVFLTLDSQQQLEAFVSDGEEVEAGKVIARMVGFADVLISAERVA
                     LNLLQRLSGIATLTNAYVKAVEGTGAQITDTRATTPGLRPLERYAVELGGGYNSRFGL
                     DDGVVVTSNHIAILGGVAGAVKSAKEKLGYLHKVAVQVSSENDLREAINAGADMLVIE
                     DLSAQEFARLASIGRELSSAITIECDGMITPENVREYAEAGAQLIRIEALTNAAPAAN
                     ISFKVQPF"
     gene            1419..2177
                     /locus_tag="VFH96_01310"
     CDS             1419..2177
                     /locus_tag="VFH96_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6972627.1"
                     /translation="MGLAEKTRHRLAQRALARQLAYQERKAEHLRGHEEEVISAMIQS
                     SRRVRDTLEAFQPIKPDARVIEVGSGAHGLIFYFGSQHGIGVDPLAVSYRSLFPRWQS
                     CAPTVAAVGESLPFRDRSFDVVICDNVVDHAESPARIVRELARILIPGGMLYFTVNVH
                     HPVYAVAAGVHSSWRAVGVPYEIGPFADHTTHLTVEAAEALFDGLPLRILSAKSNVDE
                     AKARARQKPPRHVGDRLKRVFFKNAVYEVVARRE"
     gene            2309..3100
                     /locus_tag="VFH96_01315"
     CDS             2309..3100
                     /locus_tag="VFH96_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972628.1"
                     /translation="MPVWIRNYIKVLIAVCLVVSFCLVEGNAQKRRKKHTRKSAPAAP
                     KPVITNPTIAPPDSTKAPAETGDVKIISTADQTKEQSSETLQGWKPKVTAPAPAPAEE
                     DDTQPSAIKDLTNQVNRLNNKLTQMQEDDRYQLDMERLSRAEQRAEQIRGQLMDIQGK
                     IADFEARLEQIDYALRPENIEAATQGYGSTRPEVARDTRRKQLESERGRVQAQLKLAE
                     TSRARLEVAVVNADAEVDNLRAKLEQRRAQMDAQQNDPPPKPRKP"
     gene            complement(3139..3885)
                     /locus_tag="VFH96_01320"
     CDS             complement(3139..3885)
                     /locus_tag="VFH96_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6972629.1"
                     /translation="MKKLVAVTLLLLLATTVSAQTADAAELTKLLNDFLAGASRNDVA
                     VHERFWADDLIYTRSAGRRVNKAEVMHDVRSAPAPKPSDPKTIYTAEDIRIQQYGDTA
                     VVAFRLVATTEKNVANLLNSGTFVKRNGKWQVVNWQSTRMPRTEAESKSEVTTADAAL
                     HEAIVSGDIDKLTALTDPTFVWTHGSLQSTRQQLFDDLRAKKVKYGKPDPAKVSISVY
                     GDTAVVRGESYTLTFINQLGVWRAVSLHTS"
     gene            complement(3905..4429)
                     /locus_tag="VFH96_01325"
     CDS             complement(3905..4429)
                     /locus_tag="VFH96_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972630.1"
                     /translation="MRRSYVCYIIACVIAAWTPVVTVFYPTRVTTAEVVPFPGWSNTF
                     EGKTLTPLPLNAIEKTFASDFPGRMMRFTDGEREIIVRWVTEATRMLHPASDCFQGLG
                     YTVKPLAAHRDQNGTLWASFAATKGDEHLLVYDRIVSDSGETWTDVSAWYWSALRHEG
                     SGSWWAITVAQRQR"
     gene            complement(4402..5205)
                     /locus_tag="VFH96_01330"
     CDS             complement(4402..5205)
                     /locus_tag="VFH96_01330"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HET6972631.1"
                     /translation="MKRLLLIVGLQVFAFWDVWRWYVSRAVYSWDQPWGVLALIAAIV
                     FLLASRQPGPPADRSLLLPTLLTIFYMATFLSFGPLARATVAFTALAVTTSSLRFGKS
                     FHPGLFGLFYLSLPTLPTLQFFGGYPLRVVVAEITAPILRMSGFAVIPEGTCLNWAGK
                     LIWIDAPCSGIKMLWVGLFLTFVVLCLYELPLLKTLVLLPLVGVVILVTNVFRAVALF
                     YIEAGVLNTPSWGHEYAGVIAFVLEAAGIIGIVLRLRRGNVCVVATSAT"
     gene            complement(5202..7598)
                     /locus_tag="VFH96_01335"
     CDS             complement(5202..7598)
                     /locus_tag="VFH96_01335"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HET6972632.1"
                     /translation="MEEERQYEPVQQYNPGPQYDPGPNRFTFFAGVIMPTISITVEAT
                     THICAGVFFDPIPTIWHLLFVIFVPLAQLQVWFAIRRRSPEHLALAGFLNAVAIGVSI
                     FYAIVYLPLVPLALLALLFGLGLLPLAPLLSLIASLIMRHQLKRLAAAAPRRSFALKA
                     RGLLAGLALTAAVIGMAELPATITTIGLYMATSSSPQTRANGIQFLRKYGNKDALLRS
                     CYDRTGWATDVISSAFAVQNPITPVEAQKIYYRVTGETFDASIPLERTGVRVATREII
                     NFDNNQGGEQVGGGQLAGLSLASSKLDGSVDADGGVGYMEWTLTFRNDSNVQREARAE
                     VQLPPGGVVSRLTLWVNGEEREAAFAGRGQVREAYQKVAIQQRRDPVLVTTTGRDRVL
                     VQCFPVPPGQGEMKIRFGITLPLVLEDETHAKLLLPHFVSKNFNIPEDVKHAVSVQGK
                     TAMWPINNAFLSGRREDLFSWAGTISDNELADNRSAIALNRYAVREMWTRDPFNPGFA
                     VTQSVTEYTPQYLRRIVVVVDTSAAMRVYVPQIYHAIESLPPGFDVKLVWADADDSGG
                     DVTFNGGADNAPALLQAWNLAAETAGNNAIVWIHNPQRVLLSPVDELRKRWERPYGPT
                     LYSVQIANGSDEVEKQLDGIDEVRTVARVGFLQKDLENLFSQLTGRVKTLQLVRSSKK
                     LDKRRQTFGGVETSDHLARLWANDEVTRILAPRDETLTAKARSLAIQYQLVTPVTGAV
                     VLENAAQYRASGLQPVDAGTVPTIPEPEMVALLIVAGAFMIWITYMKYRKKGRGSCTV
                     "
     gene            7778..8107
                     /locus_tag="VFH96_01340"
     CDS             7778..8107
                     /locus_tag="VFH96_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET6972633.1"
                     /translation="MSTIPQRDLYRLINDANGRGERVVVATVAHTRGSTPQQRGAKML
                     FFQNGEVAGTVGGGCIEAEVWAEAKAALRSGESRLHHFSLTADEASEEGMVCGGTMDI
                     FVEVLGN"
     gene            8113..8409
                     /locus_tag="VFH96_01345"
     CDS             8113..8409
                     /locus_tag="VFH96_01345"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HET6972634.1"
                     /translation="MTQDFIANYKAKHQHPLNRLFHSIGIPLIVISLPLFFVSWRWAL
                     ALFIAGWIFQFVGHAIEGNQPAFFKNPVYLLVGPLWLARRAASAIGFAKPKFSK"
     gene            8457..9197
                     /locus_tag="VFH96_01350"
     CDS             8457..9197
                     /locus_tag="VFH96_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET6972635.1"
                     /translation="MIEQILERGEIAVVVTLVSGPASIGAKLLVHENGTTSGSLGDLD
                     SWVIEKAPEFLASREETRMFTVAETSLLFERLQPAPRLVVCGAGHVGASLARLAAFTG
                     YHTTLIDDRAEFLKRERFPEEEIELLVADDWSSAVRKAVGNGRGVSVAVVTRGHNEDE
                     QCMHIAVDTNPDYLGLIGSKRRTSIVLDRLRDAGVSEEQLGKIHAPIGLDIGAVSPEE
                     VALAILAEIVADRRGGTGASLSTLRRKS"
     gene            complement(9399..9680)
                     /locus_tag="VFH96_01355"
     CDS             complement(9399..9680)
                     /locus_tag="VFH96_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972636.1"
                     /translation="MGHPHWNIFEWDHADRKNGNVQHFYDHGIEPHEAEECFFHEFFY
                     TRDYRRFDDVYLMDGRTNRGRKLGLVFQDKGNGVVRVFTGWDLSRKKKR"
     gene            9765..11036
                     /locus_tag="VFH96_01360"
     CDS             9765..11036
                     /locus_tag="VFH96_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6972637.1"
                     /translation="MPTEIAPVVTPSSPTARRSGMFRALSHRNYRLFWMGAFLSNVGT
                     WMQAVAQGWLVLQLTNSPFWLGLDAFMATAPGFVLTLLGGVFADRIDRRRLLLYTQVV
                     AGLAALGLAALVETSVVNRWMVLGFSFVTGCCMSLASPSYLALTYDLVGREDVANAIA
                     LNSTQFQLSRVVGPALAGVAFRVFGLAGCFFANGLSFIAVVAALWMVRMDRRPNALAV
                     SEKRRAIWRDLVEGLRYVRNRPRVSSLLLLAAVNSLFGAPYFSLVPIYARDIFHLGET
                     GLALLMGTAGAGAFLAALLVAYLGDFRRKGWFVLGGAIVFGICITGFALSSRLKLSLM
                     FLFGLGFALVCSIAITNTLLQKLVTDQMRGRVMSMFLLSFMGTMPIGNILAGTASNHF
                     GPQRTLAFGGFVVTMVATSVLIFNQRLRELH"
     gene            11110..13323
                     /locus_tag="VFH96_01365"
     CDS             11110..13323
                     /locus_tag="VFH96_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HET6972638.1"
                     /translation="MRIQTITRVLLTFSILCALQTIAGAQGVIVPGPCRRCPDPRPVT
                     LPRALPIKSIKIDTKISSQVATTHVEQVFRNDTPAILEGTYLFPIPESASIAEFAIWD
                     GDRRLVGEVRSREEARRIYDEIVRRQRDPGLLEYAGKDLFQASIFPIPPYSDKKLEIT
                     YTQVVRAEGGTVSYRYPLGTGRQLTQIGSVAGRVEVESKEPLRNVYSPTHVIDVKRSS
                     DRRSLVSFESESGKEPQDFQLFYTISKEDFGLTLLTHREAGKQGYFLLMISPKDYWSE
                     QEYSAKDVVFVVDTSGSMAEEGKMEKARSALLYGVRILRPQDRFNIISFAGEEHLMEA
                     GLIAADEQGRARGEAFVKKLKPVGGTNINQSLLASLRQFSETDRERPKMLVFMTDGLP
                     TVDETNVSRILDNVRKATRPGLRLFTFGVGYDVNTALLDKLAAENGGVADYVEPKEDL
                     EVKVSNFFSKVNYPVLTDLQLDMGGARTDLMYPRNIPDAFRGSQVTLIGRYSNEADLN
                     SIQLRLTGKAGGSTRTYTYTNLSFPLRTDANDYLPRLWATRRVGWLMEQVRSNGEQKE
                     LRDEIVDLGTRYGIVTPYTSYLALEDERMSVNAPRQSVTRRGVTGGGFGNLNSAPASA
                     PKVMNESVMVTGVDAVQQSKAQREQQDVIRIKDDDVRTDAVKRVAGKTFYLIDGVWTD
                     SEFKAESKLPETVLVFGSDEYFALLKQNTKLGSYFSLGERVVVVLDGRVYRVNAR"
     gene            13466..14446
                     /locus_tag="VFH96_01370"
     CDS             13466..14446
                     /locus_tag="VFH96_01370"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HET6972639.1"
                     /translation="MITRTLKLTRFSAALVVFLALAGSIRAQGWIPSRINTGGNDLNT
                     VYFLDSKRGWVGGDGGYLGATQDGGQTWVRQHVGTKAAINDIYFRDKDAGFLLAGNSI
                     FVTRDANNWVQSRIFLPEEFEGSDVELYSVRFSSKKKGWVVGSISTKERVVDSILVYT
                     DDGGETWRRQRTPSRVELIHIDFVSDKRGWIVGDAGTVLFTRDAGASWVKQNVGVSST
                     LYHVDFRDDRNGWVVGERGTLLRTTDGGETWTVIPTNTNVTLLSIQFLNDDEGWAIGR
                     SGTILRSSDEGKTWVPQESTTKQNLYSLNFNKKVGWVVGGAGVALRYERP"
BASE COUNT         3242 a         3926 c         4191 g         3178 t
ORIGIN      
        1 acagtgatgc tgactgcgta tcccgaaggc gcggcaagtt tgatcgacga agtcgcagaa
       61 tccgagatgc aggcgatcat cgagctgatc tcgcgcgtgc gaaacattcg ctccgaaatg
      121 aacatcaagc cgggggagcg aattccgatc gtcgttgctg cgcctgacga gagcctgcgc
      181 aaagtgttct cagcagcgcg cgaacagatc tcgcgtctcg ttcgtgcttc tgaagtcgcc
      241 gtgactgagc ggcttgaagc accgaaagcc tcggcgcgcg ccgtgttgtc tggcggagca
      301 gaactcgcag tgccgctcga aggtttgatt gacttcgaac aagagcgacg tcgtttacaa
      361 cgcgaacagg aaaaactgca agccgaagcc gcgaagctcg aagcgcaact cagcaatcca
      421 aactttgtgg agcgagcgcc agtcgagcga gtgaatgaag tgcgtgatcg catcgccgcc
      481 atcgcgcaac agtcgtctca acttcagcag acagtcgaga acctccaatg aactggatcg
      541 acgaaggcgc cctcttctca caaatcagtc tcttcctgcg tgaagatctc gggcgcggcg
      601 acatcactac acagtcgatc atcgcgcgca acacgcgtgc tcgcgctcgt ttcgtcgcgg
      661 gcgaaaacat gatcgttgcg ggtctcgaag ctgccgaaga agtgtttttg acactcgact
      721 cgcagcagca actcgaggcg ttcgtttcgg atggtgaaga ggtcgaggcc gggaaagtga
      781 tcgcgcggat ggtcgggttt gctgacgtgc tgatctctgc cgaacgtgta gcactgaatc
      841 ttttacagcg actctccggc atcgcgacgc tcacgaacgc atacgtcaaa gccgtcgaag
      901 gaactggcgc tcagatcaca gacacgcgcg cgactacgcc gggattgcgt ccactcgagc
      961 gatatgctgt cgaactcggc ggtggttaca acagcaggtt cggcctggac gatggtgtgg
     1021 tggtgacgtc gaatcacatt gcgattctcg gcggtgttgc cggagcagtg aagagtgcga
     1081 aagagaagct tggttacctc cacaaagtcg cggttcaagt gtcgagtgag aacgacttac
     1141 gtgaagcgat aaacgccggt gctgatatgc tcgtgatcga ggatctgagc gcgcaggagt
     1201 ttgcgcgcct cgcatcgatc gggcgcgaat tgtcgagcgc gataacgatc gagtgtgatg
     1261 gaatgatcac gcctgagaac gtgcgtgagt atgccgaagc aggtgcgcaa ctgattcgta
     1321 tcgaagcgct aacgaatgcc gcacccgccg cgaatatcag tttcaaagta cagccttttt
     1381 agccacaaaa aggcacaaca cataacaaaa agcaataaat gggtttggca gaaaagactc
     1441 gtcacaggct tgcgcagcgg gccctggcgc gccaacttgc ttaccaggag cgcaaggcgg
     1501 aacacttgcg cgggcacgaa gaagaggtca ttagcgcgat gattcaatcg tcgcgacgcg
     1561 tgcgcgacac tctcgaagcc tttcagccga tcaaaccgga tgctcgtgtg attgaagtcg
     1621 gcagcggtgc gcacgggctg atcttctact tcggttcgca acatggaatc ggagttgatc
     1681 cgctcgcggt cagttatcga agtctctttc cgcgctggca gagttgtgct ccgacagttg
     1741 ctgcggtggg tgagtcgtta ccgtttcgcg atcggagttt cgacgtcgtg atttgtgata
     1801 acgtcgtcga ccatgcggaa tcgccggcgc gaatcgtgcg tgagctcgcg cgtatcctga
     1861 ttccgggagg catgttgtat ttcacagtga atgttcatca cccggtgtat gcggttgccg
     1921 ctggtgtgca ctcgagctgg agggcggtgg gtgtgccgta tgagatcggg ccgtttgccg
     1981 atcacacgac gcacctcacg gttgaagctg cagaagcgtt gtttgacgga ctgccgctgc
     2041 ggattctgag tgcgaagagt aatgtcgacg aagcgaaggc gcgagcgcgc caaaagccgc
     2101 cgcggcatgt tggagacagg ctgaagcgcg tgttttttaa gaatgcggtg tatgaggttg
     2161 ttgcgcggcg tgaataacct gaatttggct gattttgctg gaacaagcgt aggaaactga
     2221 cgtggaaacc ttgaagcatc tggccagagc ttgatggcat ctggataccg agagtatctt
     2281 aggggcaggt ctgagaagga gcaccacaat gcctgtttgg attcgcaatt acatcaaagt
     2341 cctcatcgcc gtatgtctgg tcgtgagctt ctgcctcgtc gaaggtaacg cgcagaagcg
     2401 ccggaagaag catacgcgaa agtcagcgcc cgcagcgccc aagccggtga ttacaaatcc
     2461 gaccattgcg cctcctgatt ccaccaaagc tcctgctgaa accggcgacg tgaagatcat
     2521 cagcaccgct gaccagacga aagagcagtc atcagaaacg ctccagggct ggaagccaaa
     2581 agtcaccgct ccggcgccgg ctcctgctga agaggacgat acgcagccgt cggcgattaa
     2641 agaccttaca aaccaggtca acaggctgaa caacaagctg acgcagatgc aggaggatga
     2701 tcgctaccag cttgatatgg agcgactgag tcgtgctgaa cagcgtgcgg agcagatacg
     2761 cgggcagttg atggatatcc agggcaagat tgctgatttc gaagcacgac tcgagcagat
     2821 cgattatgcg ttgcggccgg agaacatcga ggcggccaca cagggttatg gaagcactcg
     2881 tccggaagtg gcgcgagaca cgcgcaggaa gcagctcgag agtgaaagag gaagagtgca
     2941 ggcgcagttg aaactcgcgg agaccagccg agcgcgtttg gaagtcgcgg ttgtgaatgc
     3001 ggacgcggaa gtcgacaacc tgcgagcgaa actcgagcaa cgtcgtgcgc agatggatgc
     3061 gcagcaaaat gatccacctc cgaagccgcg aaagccgtaa agaagtttta gccgcggatt
     3121 tacgcggatg aacgcggatt agctggtgtg gagggagacg gcgcgccaga cgcccagttg
     3181 gttgatgaag gttagtgtgt aggattcgcc gcgtaccacc gcggtgtcgc cgtaaactga
     3241 aatactcacc ttggccggat cgggctttcc atacttcact ttcttcgctc gcagatcgtc
     3301 gaacaactgt tgacgtgtgc tttgcagaga tccatgtgtc cagacgaagg ttggatcggt
     3361 tagtgcagtg agtttgtcga tgtcgcccga cacgattgct tcgtgcagcg cggcgtccgc
     3421 ggttgtgact tcgcttttgc tctcggcttc cgtgcggggc atgcgcgtcg actgccagtt
     3481 aactacctgc cacttgccat tccgtttgac gaacgtgccg ctgttcagca gattcgcgac
     3541 gttcttttcc gtcgtcgcga caagacgaaa ggccacgact gccgtgtcgc cgtactgttg
     3601 aatgcgaatg tcttcagcgg tgtagatcgt tttgggatcg cttggcttcg gcgcgggtgc
     3661 tgaacgcaca tcgtgcatca cctcggcctt gtttacacga cgaccagccg aacgtgtata
     3721 gatcagatca tcggcccaga agcgctcgtg aactgcgacg tcattgcgtg aagcgccggc
     3781 gagaaaatcg ttcaggagtt tcgtgagctc ggcagcgtcc gcggtttgcg cggaaacagt
     3841 ggtcgcgagc aaaagtaaga gtgtgacggc gacaagcttt ttcataggtg agacataata
     3901 gatctcagcg ctgtctctga gctactgtta tcgcccacca tgagccgctc ccttcgtgac
     3961 gcagcgccga ccagtaccac gccgaaacgt ccgtccacgt ttcaccggag tcagaaacga
     4021 ttcgatcgta tacgagcagg tgctcgtcgc ctttcgtcgc agcgaagctg gcccacaacg
     4081 ttccattctg atcgcgatgt gccgcgagtg gcttcaccgt gtaaccaaga ccctggaaac
     4141 aatcagacgc aggatgcaac attcgcgtcg cttcagtaac ccagcgcaca atgatttcgc
     4201 gctcgccgtc agtgaaccgc atcatccgcc cgggaaagtc agacgcgaac gttttctcaa
     4261 tcgcgttgag aggcaacggc gtcaaggtct tgccttcaaa agtgttggac caacccggaa
     4321 acggcaccac ttccgcggtc gttacccgag ttgggtaaaa gacagtcaca accggcgtcc
     4381 atgccgctat tacacaggcg attatgtagc agacgtagct acgacgcata cattccctct
     4441 tctcagccgc aacacgattc caataattcc cgccgcttcc aacacgaacg cgatcacacc
     4501 agcgtattca tggccccacg aaggcgtgtt caaaacaccg gcttcgatat aaaacaatgc
     4561 cacagcgcga aagacgttcg tcacaaggat cacaacgccg acgagcggca acagcacaag
     4621 cgtcttcagc aacggcaatt catatagaca caacaccaca aacgttaaga acagccccac
     4681 ccagagcatc ttgatcccgc tgcacggcgc gtcgatccag atcaacttgc ccgcccagtt
     4741 caaacatgtt ccttccggaa tcactgcaaa accactcatg cgcagaattg gcgccgttat
     4801 ctctgcgacc acgactcgca acggataacc accgaagaat tgcagtgtcg gcaatgtcgg
     4861 caacgagaga tagaagaggc caaacaagcc gggatggaat gactttccga aacgcagtga
     4921 gctcgtcgtc acggctaaag cagtgaatgc gaccgtggct cgcgcgagtg gaccaaacga
     4981 caaaaacgta gccatgtaaa agatcgtgag cagcgttggt agcaacagcg aacggtctgc
     5041 tggtggcccg ggctggcgcg acgcgagtaa aaacacgatc gcagcgataa gagcaagcac
     5101 gccccaaggt tgatcccacg aataaacagc gcgcgagacg taccagcgcc acacgtccca
     5161 gaacgcgaac acctgtaaac caacgatcaa taacagtcgc ttcatacggt gcaactgccg
     5221 cgaccttttt tgcgatactt catgtacgtt atccagatca tgaaggcgcc cgccacgatc
     5281 agtaacgcaa ccatctctgg ttctggaatc gtcggtacgg ttccggcgtc gactggctgg
     5341 agaccggacg cgcgatactg cgccgcattt tcgagaacaa cggcgccggt caccggcgtc
     5401 acgagttgat attgaatggc aagtgacctc gctttggcgg ttaacgtttc atctcgcgga
     5461 gctaagatgc gcgtgacttc gtcgttggcc caaagcctcg ccagatgatc ggaggtttca
     5521 actcctccaa acgtctgccg gcgtttgtcc aacttcttgc tggatctcac gagctgcaac
     5581 gtcttcacgc gcccggtaag ctgtgagaaa agattttcaa gatccttttg taaaaagccg
     5641 acgcgcgcga ccgtcctcac ctcgtcgatc ccgtcaagct gcttctcgac ttcatccgag
     5701 ccattggcga tttgaacgga atacagtgtt ggtccatatg gacgctccca acgctttctg
     5761 agctcatcta cggggctaag cagcacgcgt tgagggttgt ggatccagac gatcgcgttg
     5821 ttgccggccg tctctgccgc aagattccac gcttgtaaca gcgccggtgc attgtctgct
     5881 ccgccattaa acgtgacatc tccaccacta tcgtctgcat ctgcccaaac gagtttcaca
     5941 tcgaaaccgg gaggcagaga ttcgatcgca tgataaatct gcggaacgta aacccgcatt
     6001 gctgccgagg tatcgactac aacaacgatg cgtcgcaagt attgtggggt gtattctgtt
     6061 actgattgag tgaccgcaaa tcccggattg aaaggatccc tggtccacat ctctcttacg
     6121 gcataccggt tcaacgcaat ggccgagcga ttgtcagcca gctcgttgtc actaatcgta
     6181 ccggcccagc tgaaaaggtc ttctcgacgg ccggacagaa acgcgttgtt aatcggccac
     6241 attgcagttt ttccctggac cgagaccgcg tgcttaacgt cctcgggaat attgaagttt
     6301 ttggaaacga agtgtggcag caacagcttt gcgtgagtct catcttcgag cacgagcggc
     6361 agcgtgatgc caaagcgaat cttcatttcg ccctggccag gtggaacggg gaagcattgg
     6421 accagaacgc gatcgcgccc ggtagtggtg acgaggactg gatcgcgccg ctgttgaatc
     6481 gcgacttttt gataggcctc tctgacctgg ccacgtcctg caaacgcagc ttcacgctcc
     6541 tcaccattta cccacaaggt caaccgcgac acgaccccgc ccggcggtag ttgcacttca
     6601 gcgcgtgctt ctcgttgcac gttggaatcg ttgcgaaagg tgagtgtcca ctccatgtag
     6661 cccacgccac catcagcatc tactgaaccg tcgagttttg agcttgcgag tgacagccct
     6721 gctaactggc cgccgccaac ctgctcgcca ccctgattgt tatcgaagtt gataatttcc
     6781 cgtgtcgcta ctcttacgcc cgtgcgttcc agtgggatgg aggcgtcgaa ggtttcgcca
     6841 gtcacgcgat agtaaatctt ctgggcttct accggcgtga tgggattttg tactgcgaag
     6901 gcggagctga ttacatcagt ggcccaaccg gtgcgatcgt agcagcttcg cagcaatgcg
     6961 tccttgttgc cgtattttcg taagaactga attccgttcg ctcgcgtttg cggcgaagat
     7021 gaggtcgcca tgtataaccc gatcgttgtt atcgtggctg gtagttcagc cattccgatg
     7081 actgcggccg tcaacgccaa acctgcgagc agacctcttg ctttgagtgc aaaactgcgt
     7141 cgcggcgctg cggctgccag gcgtttcagt tgatgacgca ttatcaaact ggcgataagc
     7201 gacaggagcg gagctagcgg caacaagcca agaccaaaca gtagcgccaa tagcgcgagt
     7261 gggacgagcg gcaagtacac gatcgcatag aagatcgaga ccccgatcgc gactgcgttc
     7321 agaaaacccg cgagcgcgag atgttcaggg ctgcgtctgc gaatcgcaaa ccagacttga
     7381 agctgcgcga gcggcacaaa gatcacgaac aggagatgcc agatcgtcgg tatcggatcg
     7441 aagaacacac cggcgcagat gtgggtggtg gcttcaaccg tgatcgagat ggtgggcatg
     7501 atcacgcccg caaagaatgt gaagcggtta gggcctggat cgtattgcgg gcccgggttg
     7561 tactgctgta cgggctcgta ttgccgctct tcttccatcg tgccctcctg ttccggtaaa
     7621 gggaatgtgc gtgactttac attacaaagt aatttgccgc aagtaaaaag tgggccagcc
     7681 gtgctttctc tgcagcgaga agcaaacctt ttgtgcaaag tctcgcggtg acgtagactc
     7741 tccagcagaa aacacgaacg aagaagcaca aagttccatg tctaccattc cacaacgtga
     7801 tttgtatcgc ttgatcaatg acgctaacgg ccgtggtgag cgtgttgtcg tggccaccgt
     7861 cgcgcatacg cgtggctcga ctccgcagca acgcggtgcg aaaatgcttt tctttcaaaa
     7921 cggtgaagtc gcgggcacgg tgggcggcgg ttgtatcgag gccgaggttt gggccgaagc
     7981 gaaagcggcc ctgcgctctg gtgaatcgcg tttgcatcac ttctcactca ctgccgacga
     8041 ggcgagcgaa gaaggcatgg tctgtggcgg cacgatggat atttttgtag aggtattggg
     8101 taactaatcc tgatgaccca agatttcatc gccaactaca aagccaaaca tcagcatccg
     8161 ctgaaccgtc tcttccacag catcggcatt ccgctgatcg ttatttctct gccgcttttc
     8221 tttgtgagct ggcgctgggc gctggcgttg ttcatcgccg gctggatttt tcagtttgtc
     8281 ggacacgcga tcgaaggaaa tcaaccagcc tttttcaaga atcccgtcta tcttctggtt
     8341 ggaccgctct ggctcgcgcg ccgggcagcg tccgctatcg gtttcgccaa gccgaaattc
     8401 tcaaagtaac aacatcgatg accgaaacaa gcaatcaaac atcttccgtt acgaagatga
     8461 ttgagcaaat actggaacgc ggcgagatcg cggtggtcgt cacgctcgtc agtggtcccg
     8521 catcgattgg tgcaaaactg ctggtccacg agaacggcac gacttccggt agcctcggcg
     8581 atctggattc atgggtgatc gagaaggcgc ctgaatttct cgcctcgcgt gaagagacgc
     8641 gcatgttcac tgttgcggaa acgtcgctgc tgtttgaacg actgcaaccc gcgccgcgtc
     8701 tcgttgtttg cggcgccgga cacgtcggcg cctcactggc gcgactggct gcgttcaccg
     8761 gctatcacac gacgttgatc gacgaccgcg ctgagtttct caaacgcgaa cgcttccctg
     8821 aggaagaaat cgagctgtta gttgctgacg attggagttc cgcagtgcgt aaagcggtgg
     8881 gaaatggacg tggtgtttca gtcgcagtcg tgacacgagg tcacaacgaa gacgaacagt
     8941 gcatgcacat cgcagtggac acgaatcctg attacctcgg tctgatcggc agcaagcgcc
     9001 gcacgtccat cgtgctcgat cgactgcgcg atgcgggcgt ttcggaggag caactcggaa
     9061 agatccacgc tcccatcggt ctcgacatcg gcgccgtcag ccccgaagaa gtcgcactcg
     9121 ccatcctcgc cgaaatcgtt gccgaccgcc gcggcggcac cggcgcatct ctctcgactt
     9181 tgcggcggaa atcgtaggcg acggttattt cgacgcctgc ttcaatggcg tgtttgtctt
     9241 tcggctcgaa tttccaggga atttgggacg agacttggcc ccaggatggt gcttggatgc
     9301 acgagacatg atgtcatcaa actctcgagg actcatgttg tgatacgaag cctcgatttc
     9361 cgctcgctct gtttttgata acttggcaaa tcgctttttc atcgcttttt cttacgactc
     9421 aaatcccagc cggtaaagac gcgcacaacg ccgttccctt tgtcttgaaa aacaagtcct
     9481 agctttctac cgcgattagt tcttccgtcc atcaaataaa cgtcatcaaa gcgacgataa
     9541 tctctagtgt aaaagaactc atggaagaaa cattcttcag cttcatgcgg ttcgatgccg
     9601 tgatcataga agtgctggac attaccgttt tttcgatcgg catgatccca ttcgaagatg
     9661 ttccagtgag gatgtcccat ctttgccgcg attgtaatcg aatggggtaa aacctccaac
     9721 gagtgttagc gcttttgtaa gttcgatggt aaagattaac tttcatgccc actgagattg
     9781 cgcctgtagt cactcccagt tccccaaccg ctcgccggag cggaatgttt cgcgcgcttt
     9841 cccatcgtaa ttatcgtctc ttctggatgg gcgcgtttct ctcgaatgtc ggtacgtgga
     9901 tgcaggcggt ggcgcaagga tggctcgtgc tgcagttgac caactcgccg ttctggctcg
     9961 gactcgacgc gttcatggcg acagcgcccg gttttgttct gaccctgctt ggtggcgtct
    10021 ttgccgatcg aatcgatcga cgacgattgc tgctgtacac gcaagtcgtg gccggactcg
    10081 ccgctcttgg tcttgccgct ctggtcgaga cgtcggttgt gaatcgttgg atggtgctgg
    10141 gcttttcgtt tgtgactggc tgttgtatgt cgctggcgag tccttcgtat ctggcgttga
    10201 cttacgatct cgtcggtcgt gaagacgtcg cgaatgcgat cgcgttgaac tccacgcagt
    10261 ttcaactatc gcgcgtggtt ggaccagcgc tggccggtgt cgcgtttcga gtgtttggac
    10321 tcgcgggttg tttttttgcg aatggcttgt cgttcatcgc ggtggtggct gcgctttgga
    10381 tggtgcgaat ggaccgacga cctaatgctc tcgcggtttc cgaaaagcga cgcgcgatct
    10441 ggcgcgatct ggttgagggt ctgcgttacg ttcgcaacag gccgcgcgtt tcgtcgttat
    10501 tgctactcgc agcagtgaac agtctgtttg gcgcgcctta cttcagtctc gtgccgatct
    10561 acgcgcgcga catcttccat cttggcgaaa cgggactggc gttgttgatg ggtacggcag
    10621 gagcgggcgc gttcctcgct gcgttgctgg tagcttatct cggcgacttc agaaggaaag
    10681 gctggtttgt tctcggaggc gcgatcgtgt tcgggatatg tatcaccgga tttgcgctgt
    10741 cttctcggct gaagttgtcg ctgatgtttc tcttcgggct cggctttgcg ctggtatgct
    10801 cgatcgcaat caccaacact ctcctgcaaa agctcgttac cgatcagatg cgtggccgtg
    10861 tgatgagcat gtttctgctt tcgtttatgg gcacgatgcc gatcggaaac atcctcgcgg
    10921 gcactgcctc gaatcacttc ggcccgcagc gcacgctcgc ttttggcgga ttcgtcgtga
    10981 ccatggtggc cacgagcgtg ctgattttca atcaacggct acgggaactg cactaatcct
    11041 aaaatgcaag attgaccgaa agcgcccgtt ctacctccca gcaatgttaa ttttcccgga
    11101 gaatcctgta tgcgaattca aactatcacg cgggttttgt tgaccttttc gatcctctgc
    11161 gccctccaga caatcgccgg cgcgcaaggc gtcatcgttc ccggcccgtg ccgccgctgc
    11221 ccggacccgc gtccagtcac tctcccccgc gcgctcccca tcaagtcgat caagatcgac
    11281 accaaaatca gctcgcaagt cgccaccact cacgtcgaac aggtctttcg caacgacacc
    11341 ccggcgatac tcgaaggcac atatttgttt ccgattcccg aatcagcctc gatagccgag
    11401 tttgcgatct gggatggcga tcgacggctc gttggtgagg tcagatcgcg cgaggaagca
    11461 cgccgtattt acgacgagat cgtccgccgg caacgcgatc cgggattgct cgaatacgcc
    11521 ggcaaagact tgttccaggc cagcatcttt ccgatccctc cgtactccga taagaagctc
    11581 gagataactt atacgcaggt cgtgcgcgct gaaggcggga cggtttcgta tcgctatcca
    11641 cttggcactg gtcgccaatt aactcagatc ggctcggtag ctggtcgagt cgaagtcgaa
    11701 agcaaagaac ctttgcgcaa cgtttactcg cctactcacg ttatcgacgt gaagcgcagc
    11761 agcgaccgac gctcgctcgt gtcgttcgag agcgagagcg gcaaggagcc gcaggacttt
    11821 caactgttct acaccatctc gaaagaagac ttcggcctga ccctgttgac gcaccgcgaa
    11881 gccggaaaac agggttactt cctcctgatg atctcgccca aagactactg gtcggagcag
    11941 gaatactcgg ctaaggacgt cgtcttcgtc gtcgacacat cgggctcaat ggctgaagaa
    12001 gggaagatgg agaaagctcg cagcgcactg ctgtacggcg taagaatcct gcgcccgcaa
    12061 gatcgtttca acatcatctc gttcgcaggg gaagagcacc tgatggaagc gggtttgatc
    12121 gcagccgatg agcaaggtcg cgcgcgcggt gaagcgttcg tgaagaaatt gaagccggtg
    12181 ggcggaacaa acatcaacca atcgttactc gcgtcactgc gtcaattcag tgaaacagat
    12241 cgcgagcgac cgaagatgct cgtgttcatg actgacggtt tgccgacagt cgatgagacg
    12301 aacgtttcgc gcatactcga caacgtgcgc aaagcgacga gaccgggcct gcgattgttt
    12361 acgttcggtg tcggttacga cgtgaacacg gcattgcttg ataagctcgc agcggagaat
    12421 ggcggcgttg ctgattacgt cgagccgaaa gaagatctcg aagtgaaagt ctcgaatttc
    12481 ttttcgaagg tcaactatcc ggtgctcacg gatctacaac tcgacatggg cggcgcccga
    12541 acagacctga tgtatccgcg caacattccc gatgcgtttc gcggttcgca ggtgacgctg
    12601 atcggccgct acagcaacga agccgatctc aattcaatac aactgcgctt gactggtaaa
    12661 gcaggcggct caacgcggac ttacacatac acgaacctga gcttcccttt acgcaccgac
    12721 gcaaacgatt acctgccgcg cttgtgggcg acgcgccggg ttggctggtt aatggagcaa
    12781 gtgcgctcga atggcgagca aaaggagttg cgcgacgaga tcgtcgatct gggcacgcgc
    12841 tacggaatcg tgacgcctta cacgtcgtac ctggctttgg aagatgagcg catgtcggtc
    12901 aacgcgccga gacaaagtgt gacccgccgg ggagtcacgg gcggtggatt tggaaacctg
    12961 aattctgcgc ccgcgtcagc tccgaaagtc atgaatgaga gcgtgatggt gacgggtgtc
    13021 gatgctgtgc agcaaagcaa ggcgcaacgc gagcagcagg atgtgattcg tatcaaggac
    13081 gacgacgtgc gtacggacgc tgtgaagcgc gtcgcaggca agacgttcta tctgatcgac
    13141 ggcgtgtgga ctgattcgga gttcaaagct gagtcgaagc tgccggagac agtgctggtg
    13201 tttggcagtg atgagtattt cgctctgctg aagcagaata cgaagctcgg gagttacttc
    13261 tcgttaggtg agcgagtcgt tgtcgtgctc gacggacgcg tttaccgagt gaacgcacga
    13321 taaagcacag aaccgagcgc gatagcgccg ggtcaaagat cgcattcacg accgttgatg
    13381 catttgaccc ggcgctaccg cgctcggttc tgtttgtttg cctcggggcc tgttttaggg
    13441 tacaaaactg aaaacaaaat cagttatgat taccagaacc ctgaagctaa cacgcttttc
    13501 tgctgctctt gttgtgtttc tggctctggc tggatcaata cgcgcccagg gctggatacc
    13561 ctccaggatc aacactggtg gaaacgatct caacactgtc tactttctcg acagcaaacg
    13621 tggctgggtc ggtggtgacg gcggttacct gggtgccacc caggacggcg gtcaaacgtg
    13681 ggtgcgccag cacgtcggca cgaaagccgc catcaacgac atctactttc gcgacaaaga
    13741 cgctggcttt ctgctcgccg gcaattcgat ctttgtcact cgcgacgcca acaactgggt
    13801 gcagtcgcga atcttcctgc cagaggagtt cgaaggttcc gatgtcgagt tgtacagcgt
    13861 gcgcttttcg agcaagaaaa agggctgggt cgtcggctct atcagcacga aggagcgcgt
    13921 ggtcgacagc atcctcgtct acacggacga tggcggcgaa acgtggcgca ggcagcgcac
    13981 gccgagtcgc gtggagttga tacacatcga cttcgtcagc gacaagcgcg gttggattgt
    14041 gggcgacgcg ggaacggttc tcttcacgcg tgacgcgggc gcgagttggg taaagcagaa
    14101 tgtgggcgtc agcagcacgc tctatcacgt ggattttcgc gacgacagaa acggctgggt
    14161 cgttggtgag cgtggaacat tgttgcgcac tactgacggt ggtgagacgt ggacggtgat
    14221 ccctaccaac acgaatgtga cgttgttgag cattcagttt ttaaacgatg atgaaggttg
    14281 ggcaatcggc cggtcgggca cgattctcag gagcagtgac gaaggaaaga cgtgggtgcc
    14341 acaagaatcg acaacgaagc aaaaccttta ctcgctgaat ttcaacaaaa aggtggggtg
    14401 ggtagttggt ggtgccgggg tggcgctgcg ttacgagcgc ccatagttcg tgcaagcaac
    14461 ctgtgaagtg ctgaaagaca tctgaacgtc gaggttaacg gatcgtcgca agaatagtga
    14521 cgaccataac actcggt
//
LOCUS       DASSFQ010000038         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2102194, whole genome shotgun sequence.
ACCESSION   DASSFQ010000038 DASSFQ010000000
VERSION     DASSFQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102194"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2141)
                     /locus_tag="VFH96_01375"
     CDS             complement(<1..2141)
                     /locus_tag="VFH96_01375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET6972640.1"
                     /translation="MRTGFTRFFMINRISFLFILKYLVNPVFLLFLFSVPLVAQRDLT
                     RYVNPFIGTGGHGHTFPGAIVPYGMVQLSPDTRLTGWDGCSGYHYSDSKIYGFSHTHL
                     SGTGISDYGDILLIPSVGRNYSGRFQHRNETATPGYYSVKLDDENILVELTTTARVGM
                     HRYTFPATDDANIVIDLAHRDKVLDSDLRVTSKTTVVGWRRSQAWAKDQIVYFALEFS
                     QPFTAYGQADKPRKTFFRFDTRSGAPVLVKVGISAVDIDGALKNLRTEISHWDFDRVK
                     ADAKAAWNAELNKIAVSGGTDSQLTNFYTSLYHVMTVPNLFMDVDGRYRGRDFKTHVA
                     NGFSNYTVFSLWDTFRAAHPLYTIIDRKRTRDFIKTFLVQYEQGGRLPVWELAANETD
                     TMIGYHAVSVIADAAMKGVEGFDLNEAFIAMKHSAELREHRGLAAYIDHGFISTEDER
                     ESVSKVLEYAYDDWCIAQVARMLGKKEDYERYNRRAQSYKNVFDRSSGFMRPRSNGGW
                     VEPFDPREVTFAFTEANAWQYTFFAPHDISGLIALMGGRAQFASKLDRLFTAESRTTG
                     REQVDITGLIGQYAHGNEPSHHMAYLYDYVGQPWKTQSRVASIMDQFYKPEPDGLIGN
                     EDCGQMSAWYVLSAAGFYPVTPGSNVYAIGSPLFPEVRFNLENGKMFVVRTVNGSDRN
                     VYIQSARLNGKAYNKSFLSHQDLMNGGELLFT"
     gene            complement(2138..>3250)
                     /locus_tag="VFH96_01380"
     CDS             complement(2138..>3250)
                     /locus_tag="VFH96_01380"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6972641.1"
                     /translation="KEPQWVTVGAALRGRPSDNQYEGWPQRATPTVVSNLAEVLKTLA
                     DVPAVSGHERPVREAIIAALPAWARPLTRTDNEGNLILEVGPDRDPLMFIAHQDEVGF
                     EVTDVTADGIVSLRTRGGLFQSLWEGQPALLHFDRAGAAPLHGVFVPRETATNKQPEA
                     LTAWFGVDGAALKQLGVTSGLSVTAYKQATRIGNTRFTARALDDRAGSTALVLAAGRI
                     QPAVLKRKVIFAWSVREETGLEGARELAKQYASKVKRVYSIDTFVSSDSPAETTRFAH
                     APLGQGAVIRGLDNSAVAPPAELDRILNIARMQSIPIQVGATNGGTDGSDFVRYGVLH
                     VSLSWPGRYSHSPVEVLDLRDLQALERLVYAIATAQ"
BASE COUNT          731 a          925 c          884 g          710 t
ORIGIN      
        1 gtaaacagga gctcgccacc attcatcaga tcctgatgcg acaggaacga cttgttataa
       61 gccttcccat tcaatctcgc tgattgaatg tagacatttc gatctgaacc attaacagtg
      121 cgaactacaa acatctttcc attctccaaa ttgaatcgca cttcgggaaa caacggcgat
      181 ccgatcgcat atacatttga acccggcgtc accggataaa atcccgcagc gctcaacaca
      241 taccacgccg acatctgccc gcagtcttcg ttgccgatca aaccgtcagg ctcaggtttg
      301 taaaactggt ccatgatcga cgcgacgcgc gactgcgtct tccacggctg gcccacgtaa
      361 tcgtaaagat aggccatgtg atgactcggc tcattgccat gcgcgtactg cccgatcaga
      421 ccggtgatgt cgacctgttc gcgcccagtc gtccggctct ctgctgtgaa caaccgatcg
      481 agcttgctcg caaactgcgc acgcccaccc atcagtgcga tcaatccact gatatcgtgc
      541 ggagcgaaga acgtgtactg ccacgcgttt gcttcagtaa acgcaaacgt cacctcgcgc
      601 ggatcgaacg gctcaaccca gccgccgttg ctgcgcgggc gcatgaagcc ggacgaacga
      661 tcaaagacat tcttgtacga ctgcgcgcgc cggttgtagc gctcgtagtc ttcttttttt
      721 ccgagcatcc gcgcgacttg cgcgatacac caatcatcgt acgcatactc cagcactttc
      781 gagactgact cgcgctcgtc ttccgtcgag ataaatccgt ggtcgatgta cgcagccaac
      841 ccacgatgct cgcgcagttc cgcgctgtgc ttcatagcta taaatgcttc gttgagatca
      901 aaaccctcga cgcccttcat cgccgcatcg gcaatcacgg aaacagcgtg ataaccgatc
      961 atcgtgtcag tctcgtttgc ggcaagctcc caaacgggca gacgtccgcc ctgctcgtac
     1021 tgcacgagga acgtcttgat gaaatcgcgc gtgcgtttcc gatcgatgat ggtgtagagc
     1081 gggtgcgccg cgcgaaacgt gtcccagagc gagaagactg tgtaattcga aaaaccgtta
     1141 gcgacgtgcg ttttgaaatc tcgaccgcga tagcgcccat cgacgtccat aaaaagattc
     1201 ggcacggtca tcacgtgata gagcgaggtg tagaagttgg ttaactgtga atccgtaccg
     1261 ccgctcactg cgatcttatt aagctcagca ttccaggctg ctttcgcgtc agctttcacc
     1321 ctatcgaaat cccaatgact gatctcggtg cgcaggttct tcaatgcgcc gtcgatatca
     1381 acagcggaga tgccgacctt gacgagaaca ggcgcaccag agcgcgtgtc gaagcggaaa
     1441 aaagtttttc gtggtttgtc agcctggccg tatgcggtaa agggctgcga aaactcaagg
     1501 gcaaagtaaa caatctgatc tttggcccag gcttgcgaac gtctccatcc gacaactgta
     1561 gttttgcttg tgacgcgaag atctgagtcg agtactttgt cgcgatgtgc gaggtcgatg
     1621 acgatgttgg catcgtccgt tgcagggaat gtgtatcgat gcattccgac gcgcgcagtg
     1681 gtggtgagct cgacgagaat gttttcgtcg tcgagcttca ctgaatagta gccgggcgtg
     1741 gctgtttcat ttctatgttg gaagcgtccg gagtaattgc gtccaaccga cggtatcaac
     1801 aagatgtcac cgtaatcgga gatgcccgtg ccgctgagat gtgtgtgact gaatccatag
     1861 atcttcgaat cggagtagtg ataaccggag caaccatccc agcctgtcag tcgcgtatca
     1921 gggctgagtt ggaccatgcc gtacggcacg atcgcacccg ggaacgtgtg accgtgcccg
     1981 cccgtgccga tgaaggggtt cacatagcgg gtaagatcgc gttgtgccac gaggggcact
     2041 gagaacaaaa acagcaggaa gacaggattt acaagatatt tcaagataaa caagaacgaa
     2101 atcctgttaa tcatgaagaa tcttgtaaat cctgtcctca ttgcgctgtg gcaatcgcgt
     2161 agacgagtcg ctctaacgcc tgcaggtctc tgagatcgag gacttcgacg ggcgagtgac
     2221 tgtagcgtcc gggccacgac aaactcacgt gcaacacacc ataacgcacg aagtctgatc
     2281 catccgtgcc gccattcgtc gccccaacct ggatcggtat cgattgcatc cgtgcgatgt
     2341 tgaggatccg atcgagttca gcgggcggcg cgacggcgga attgtcgaga ccgcgaatca
     2401 ctgcgccttg tccaagcggc gcgtgcgcga accgcgtcgt ctcagccggt gagtcagacg
     2461 agacgaacgt atcaatactg taaacgcgct tcactttcga agcatattgt tttgcgagtt
     2521 cccgtgcccc ttcgagtccg gtctcttcac gcactgacca cgcgaagatc actttgcgtt
     2581 tgagcacggc cggctggatt cgtcccgcgg cgaggacgag cgctgtcgat ccggcgcgat
     2641 catcgagcgc gcgtgcagtg aagcgagtgt tgccgatacg cgtggcctgc ttgtatgcgg
     2701 taacagatag ccctgacgtt acaccgagct gtttcaaagc agcgccatcc acgccaaacc
     2761 atgccgtcaa tgcttccggt tgtttgttgg tcgcggtttc tctcggcaca aacacaccgt
     2821 gcaacggcgc ggcaccggcg cgatcgaaat gaagcaacgc cggttgacct tcccacagcg
     2881 actgaaatag tccgccgcgc gtgcgtagtg aaacaatgcc atctgcggtc acgtccgtca
     2941 cttcgaagcc gacttcatcc tggtgcgcga tgaacatcaa tggatcgcgg tctggtccaa
     3001 cttcgaggat gagattgccc tcgttgtcag tgcgggttaa gggacgcgcc cacgcaggca
     3061 gtgcagcaat gatcgcttcg cggaccgggc gctcgtgacc tgaaaccgcc ggaacatcag
     3121 ccagcgtctt gagcacctcc gccaggttgg aaacaactgt aggggtggcc ctctgtggcc
     3181 acccttcgta ttggttatcg gacgggcggc cacggagggc cgcccctaca gttacccatt
     3241 gaggttcttt
//
LOCUS       DASSFQ010000039         5661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2102243, whole genome shotgun sequence.
ACCESSION   DASSFQ010000039 DASSFQ010000000
VERSION     DASSFQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102243"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VFH96_01385"
     CDS             <1..758
                     /locus_tag="VFH96_01385"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6972642.1"
                     /translation="LEGMGSVVDRKLGRTVDPRSGLTTSKLIERMKRLIDERVRDEGN
                     KGRIAPHHMVLKVEWGTHSEAPPEILGDLKNEVLAAAIDHINDHRYRTLAPVTVDAEV
                     DIFTTGISVAPNFGEFEDDLRRQDEEMRAAKLGVPIPGAAPPMPDIQVIARVTLPNGT
                     REIPLIFKPGGRRLNVGRASDNDLTLNDTSVSKIHAALLMSGEGTLLVADTGSTNGTY
                     LNGRRISYGESRLIEDGDVVGFGDVEVRLRKSE"
     gene            767..2602
                     /locus_tag="VFH96_01390"
     CDS             767..2602
                     /locus_tag="VFH96_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET6972643.1"
                     /translation="MNLVTILLLLFQTPTPSPLPTPHVPERTPALFTLIYVGGLGLII
                     LLLLLGLLRSRKQNAPPIPEDLPKEVRKRLGSHSTNRGLRALRWLFVLLAIGLFSFHI
                     YWAHYASENNEKFQELSYKDLRARRLSESTLRGWIFDRSGRVDQPLAYYKRDSSGAIK
                     REYPMDKAMAHIFGSDRGDPGLERALFGVESGVLPEALEVVKGKEVEFRGNQDVRLTI
                     DRGLQQAAVDQLKGKHGAVVILNPQTGDVLALYSEPSYSLEEVDDEATWIRLEANERD
                     KPLVSRALGAYYIPGSTFKTVTMTAAFLAGEQDTEFTCSGGGYYAAPGANVIFDDGGP
                     GEVHGRIGIDHAYEVSCNQYFAQMGVKLGAEKLKRAAQLLGIGAYDTPSEALRGRKLP
                     QIWDASTDAVKRALAPREATIVTGKQVTRYDLALMGYGQGYAGQMTPFQMALAASAIG
                     NMEGKLMKPRIEMNVAPQPFNQVMPASTAARMRQIMGLVTGGASGTARGVFGPVKAAG
                     INTGGKTGTAQKVVPLYDPKTGEMKTRHKVEKDNKGNVIREYDEVIMDDEHPRIDGWF
                     LCLAPIERPQLAMAVIVEGGGYGSRSAAPIAAALVLKARDLGYFK"
     gene            2674..4365
                     /locus_tag="VFH96_01395"
     CDS             2674..4365
                     /locus_tag="VFH96_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET6972644.1"
                     /translation="MKNRASSHLLILFLILGLEITGYFAVHRAGLLRGYETSLIGAVR
                     DLMMFVPLVFLVLWLSRAKRFAGNWVLFTTAILLFSFGMLIQYRLYSDPEYNARNKAQ
                     AREEKMQALRTRYIMENYDPVKKQLMGLPPTPAQPIDINQLPKKESNYSLWNALTSSY
                     TWIPVLSFLAFAIAYSLCARDGFLLWLQRNSFIIVLFTLLPLAMAVVTSSAGKALGNM
                     TPWEPSKIPFLVGFAGILTARYKDLSETYWGIPRARDIVPLLVMAIMPFIPFFALKDF
                     GQMLIFSGAYTTLYLVAVRRWPQLLVFVGSVVIVIGILVVGALPRDIQEKVPLLPTIA
                     RPISAALPSRIQQRFHLWLDGFDPPSPEVSWWKKDYEDALSRDPGMKQLAEQSPAMQK
                     TVNVDIWFDRLAFQPAQAQFGIDSGGTTGRGFGLGFPEVIPIADSDYVYAAIAEETGL
                     AGGAVIILAIIIFVVAGVRTSLEARDMFTKLCAAGLTAFVGFQALVNIGGILRALPMT
                     GITLPFVSHGGFSLITSFAMLGMLMAFSNRNARDRVIADRPVAKSSSPLKLEAVQ"
     gene            4362..>5661
                     /locus_tag="VFH96_01400"
     CDS             4362..>5661
                     /locus_tag="VFH96_01400"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET6972645.1"
                     /translation="MSEETKTLEVQSAALSDRGLNERRPLNEDAFLHDPERTIFAVAD
                     GVGGAEAGEVASQTAIEVLDEAFRHQTSDADIEDLMELAIQRANASIHQMAQEHAKFS
                     MMATTIVALHIKGNIATFGHVGDSRLYRLTPDGQLHRETEDHSIVEEEVRAGRMTPEQ
                     AANHPSKNVISRALGAEQGVEVDMKTIEVEDGTEFLLCTDGITRHVPDNELRQLMVTS
                     NNLDELCSELKRRCYERGAEDNLTVIAVRVGEHLKAGERSADLEPTISPESNAVYAVP
                     SGNGSPLADTTFMPASRIAFPGPPSSPAAPAQVTPVSSEPLNVADPRAKSGGGGRVFV
                     ALIVLLLIAGAFYAGARYKERIPFLASKNPQPAQVVAPTATPAPEEPFVQFERARRKV
                     DQDPRAWLKDDIGKELVKAGVQNPLDSPNAEFLYLYGRANL"
BASE COUNT         1216 a         1594 c         1648 g         1203 t
ORIGIN      
        1 tgcttgaagg aatgggcagc gtcgtcgatc gcaagctcgg tcgcaccgtc gatccgagaa
       61 gcggactcac cacctcgaag ctcatcgagc gtatgaagcg cctcatagac gagcgcgtcc
      121 gagacgaagg taataaaggg cggatcgcgc cgcatcacat ggttttgaaa gtcgagtggg
      181 gcacgcattc agaagcaccg cccgagattc tcggcgatct caagaacgaa gtgctcgccg
      241 ccgccatcga tcacatcaac gatcatcgtt atcgcacact cgcgcctgtc accgtcgacg
      301 ccgaagtcga catcttcacg accggaatct cggtcgctcc gaacttcggc gagtttgaag
      361 acgatctcag gcgacaggac gaagagatgc gcgccgcaaa actgggcgtg ccgattcccg
      421 gcgccgcgcc gccgatgcct gacatccagg tgatcgctcg cgtcacgttg ccaaacggca
      481 ctcgcgagat accgctaatc ttcaagcctg gtggccggag gctcaacgtc gggcgcgcct
      541 ccgacaacga tttaactctg aacgacacga gtgtctcaaa gatccacgct gcactattga
      601 tgagcggtga aggcactctc ctcgtcgccg ataccggctc gaccaacggc acttatctca
      661 acgggcgacg catctcatac ggcgaatcca gattgatcga agacggtgat gtagttggct
      721 ttggagatgt agaagtacga ctgcgtaaaa gtgagtagcg aactggatga acctggtcac
      781 aattcttcta cttctcttcc aaactcccac gccttctccc cttccaactc cgcatgtgcc
      841 tgagcgcacg ccggcgctgt tcacgctgat ctacgtcggc ggcctcggac tcataattct
      901 gctgctgctg cttggacttc ttcgcagtcg gaaacaaaac gcgccgccga ttcccgaaga
      961 cctgccgaag gaagtcagaa aacgactcgg atcgcattcg actaatcgtg gtctgcgcgc
     1021 gctgcgctgg ctcttcgtac tgctcgcgat cggtctcttc tcatttcaca tttactgggc
     1081 gcactacgct tccgagaata acgagaagtt tcaggagctt agttacaaag acttgcgcgc
     1141 ccgtcggctg tctgaatcga ctctgcgcgg ctggatcttc gatcgcagcg gtcgcgtgga
     1201 ccaaccactc gcctattaca aacgcgattc cagcggcgct atcaagcgcg agtacccgat
     1261 ggacaaggcg atggcccata tcttcggatc cgatcgcggc gatccgggtt tggagcgcgc
     1321 gttgttcggt gtcgagagcg gtgtacttcc cgaagcgctc gaagtcgtta aaggcaaaga
     1381 ggtcgaattc aggggcaatc aggacgtgcg cctcacgatc gatcgcggct tgcaacaggc
     1441 tgctgtcgat cagttgaagg gcaagcacgg cgctgttgtc atactcaatc cgcagacggg
     1501 cgacgtgctc gcactctaca gcgagccttc ctacagtctc gaggaagttg acgatgaagc
     1561 gacgtggatt cgtctcgaag caaacgagcg cgacaagcca ctcgtgagtc gcgctctcgg
     1621 tgcttactac attcccggct cgacgttcaa aacggtgacg atgaccgccg cgtttctcgc
     1681 tggcgaacag gatacggagt tcacctgcag cggcggcggt tactatgccg cgcccggcgc
     1741 gaatgtgatc ttcgacgatg gtggaccagg cgaagtacac ggacggatcg gcatcgatca
     1801 cgcatatgaa gtttcgtgta accaatactt cgcgcagatg ggtgtaaagc tcggtgcgga
     1861 aaaactgaaa cgggcggcgc agttgctggg catcggtgct tacgatacgc cgtcggaggc
     1921 tttgcgtgga cgcaagctgc cgcagatctg ggatgcgagc actgacgcag tgaagcgcgc
     1981 gctcgcgccg agagaagcga cgatcgttac aggcaaacaa gtgactcgat acgatctcgc
     2041 cttgatgggt tatggacagg gttacgccgg acagatgacg ccgtttcaaa tggcgttggc
     2101 ggcgtccgcg atcggaaaca tggaaggcaa gttgatgaag ccgcgcatcg agatgaacgt
     2161 ggcgccgcag cctttcaatc aggtgatgcc ggcctcgacg gcagccagga tgcgacaaat
     2221 catggggctc gtgaccggcg gcgcgagcgg cacggcgcgt ggcgtgtttg gaccagtcaa
     2281 agcggcgggc attaacactg gcggcaagac cggaacggcg cagaaagtcg tgccgcttta
     2341 cgatccgaag acaggcgaga tgaagacgcg acacaaggtc gagaaggaca acaaaggcaa
     2401 tgtcatccgc gagtacgatg aagtgatcat ggacgacgag cacccgcgca tcgatggctg
     2461 gtttctctgt ctcgctccga tcgagagacc acagctcgcg atggcggtaa tcgtcgaagg
     2521 cggaggttac ggctcgagat cagcggcgcc gatcgccgca gcgctcgtgt tgaaagcgag
     2581 agatctgggc tatttcaaat agatttgagc cgcggatgaa cgcggattaa cgctgatctg
     2641 cgtttatccg cgaaaatccg cggctaaata aaaatgaaaa acagggcgtc atcacattta
     2701 ctaattctct tcttgatcct cggcctcgag atcaccggtt actttgccgt gcatcgcgcc
     2761 ggattgttgc gtggttacga gacgtcgctc atcggtgcgg tgcgcgacct gatgatgttc
     2821 gttccgttgg tcttcctcgt cctctggctt tcacgtgcca aacgatttgc cggcaactgg
     2881 gtgcttttca ctaccgcgat tctcctcttc tcgttcggca tgttgatcca gtatcgcctc
     2941 tacagcgatc cggaatacaa cgcgcgcaac aaagcccagg cgcgcgagga aaagatgcag
     3001 gcgctacgca cgcgctacat catggagaac tacgatccgg tgaagaagca gttgatggga
     3061 ctgccgccga cgcccgcgca gccaatcgat atcaaccagc ttccgaagaa agagtcgaac
     3121 tactcgctct ggaacgcgct cacttccagt tacacatgga tccctgtgtt gtcgttcctg
     3181 gctttcgcaa tcgcctactc gctctgcgcg cgtgatggct ttctgttatg gctgcaacgc
     3241 aatagtttca tcatcgtctt gtttaccctc ttaccgttgg cgatggctgt cgttacttca
     3301 agcgcgggta aagcgctcgg caacatgacg ccgtgggagc cttcgaagat tcccttcctc
     3361 gtcggcttcg caggcatcct gactgcgcgc tacaaagatc tctctgaaac ttactggggc
     3421 attccacgcg cgcgcgacat cgtgccgctc ctcgtgatgg cgatcatgcc gttcattccc
     3481 ttcttcgcgc tgaaagactt cggacagatg ctgatcttca gcggcgcgta cacgacgctc
     3541 tacctcgtcg cggtccgtcg ttggccgcag ttgctggtgt tcgtcggctc ggttgtgatc
     3601 gtgatcggca ttctcgtcgt tggggcactg ccgcgtgaca ttcaggagaa ggtgccgtta
     3661 ctgccgacga tcgcacgtcc catcagcgct gcacttccgt cgcgtattca acaacgcttt
     3721 cacctctggc tcgacggttt cgatccaccg tcgccagaag tttcgtggtg gaagaaagac
     3781 tacgaagatg cactctcgcg agatcctggt atgaaacaac tggccgagca gagtcccgcg
     3841 atgcagaaga ctgtcaacgt cgacatctgg tttgaccgtc tcgcgtttca accagcccag
     3901 gcgcagtttg ggatcgattc aggtggaact actggacgcg gctttggtct aggttttccg
     3961 gaagtgattc cgattgccga ctcagactat gtttacgcgg caattgcaga ggaaaccggc
     4021 ctcgcaggcg gcgctgtgat catcctcgcg atcatcatct tcgtggtcgc aggggttcgc
     4081 acttcgctgg aggcgcgtga tatgtttacg aagctgtgcg cagcaggtct gacagcgttt
     4141 gtaggttttc aagcgctggt gaacattggc ggtattttga gagctttacc gatgactgga
     4201 atcacgctcc cgtttgtgag ccatggtggt ttcagtctga ttacaagctt tgcgatgttg
     4261 gggatgctga tggcattttc aaaccgaaat gcccgcgatc gtgtcatagc ggataggccg
     4321 gtggctaaga gttcttctcc ccttaagctg gaggctgtgc aatgagcgaa gaaaccaaaa
     4381 ccctcgaggt ccaatctgcc gccctgtcgg atcgtggcct caacgagcgt cgcccgctga
     4441 atgaagacgc tttcttgcac gatccggagc gcacgatctt cgctgtagca gacggcgtcg
     4501 gtggcgctga agctggtgaa gtcgcgtcgc agactgccat cgaagttctc gatgaggcat
     4561 ttcgtcacca gacaagcgac gctgatatcg aagacttgat ggagctcgcg attcagcgcg
     4621 ccaatgcttc gatccaccag atggcccagg agcacgcaaa gttttcaatg atggcgacga
     4681 ccatcgtcgc gcttcatatc aagggcaaca tcgctacgtt cggccacgtt ggtgattcca
     4741 gactttaccg tctcacacct gacgggcaac tacatcgcga aactgaagac cactcgatcg
     4801 ttgaagagga agtgcgcgcc ggacgcatga cgccggaaca ggcggcaaat catccgagca
     4861 agaacgtgat cagtcgcgca ctcggcgccg agcaaggcgt cgaagtcgac atgaagacga
     4921 ttgaggttga agacggtacc gagtttttac tttgcactga cggcatcacg cgtcacgttc
     4981 cagataacga gctgcgacag ttgatggtca ccagcaacaa cctcgatgag ctgtgcagcg
     5041 agttgaaacg acgctgttac gagcgtggcg ctgaagataa tttgactgtg attgcggtgc
     5101 gtgtcggcga gcatctcaag gccggcgagc gctctgcgga tctcgagccg acgatcagtc
     5161 ctgagtccaa cgctgtttat gcagtgccgt caggaaacgg ctccccgctt gctgatacga
     5221 ctttcatgcc ggcatcgcgt atcgcgtttc ctggtccgcc gagtagtccc gcagcccccg
     5281 ctcaggtaac gccagtaagc tccgagccat tgaacgtcgc cgatccaaga gcaaagagtg
     5341 gtggcggcgg acgcgtgttt gtagctctca ttgtgttgct gctgatcgcg ggagcgttct
     5401 acgcgggtgc acgatacaag gaacgaattc ccttcctcgc gtcgaagaat cctcagccgg
     5461 cgcaagtggt agcgccaacc gcgacgcccg cgcccgaaga accgttcgta cagtttgaga
     5521 gagcgcggcg gaaggtcgat caggatccgc gtgcgtggct caaggatgac atcggcaaag
     5581 agctcgtgaa ggctggagtt cagaacccac tcgattcccc gaatgcggag tttttgtatc
     5641 tctacggtcg cgcaaatctg c
//
LOCUS       DASSFQ010000040         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2102717, whole genome shotgun sequence.
ACCESSION   DASSFQ010000040 DASSFQ010000000
VERSION     DASSFQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102717"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1237
                     /gene="gnd"
                     /locus_tag="VFH96_01405"
     CDS             227..1237
                     /gene="gnd"
                     /locus_tag="VFH96_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET6972646.1"
                     /translation="MQIGMIGLGRMGANMVRRLLRGGHECVVNDRSADAVEALLAEGA
                     AGATSLDGFIAKLKSPRTVWLMIPAALVDTILNDLTSVLSAGDIIIDGGNSYYIDDIR
                     RARELKAKGIHYVDVGTSGGVWGLDRGYCQMIGGEKEIVTYLDPIFKTLAPGSGSISP
                     TPGRAADAGTAGEGYLHCGPSGAGHFVKMVHNGIEYGLMAAYAEGLNVLHHANEGKGE
                     RAADAETTPLRYPDHYQYDFNLADITEVWRRGSVIASWLLDLTAIAFAESPQLEGFSG
                     RVSDSGEGRWTINAAIEEGVPVPVLSAALFQRFSSRGEADFANKILSAMRLQFGGHVE
                     RK"
     gene            1275..>2447
                     /gene="zwf"
                     /locus_tag="VFH96_01410"
     CDS             1275..>2447
                     /gene="zwf"
                     /locus_tag="VFH96_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6972647.1"
                     /translation="MSAIVVEPSPVQYEQPTLPQAEPCVVVIFGATGDLTKRKLMPAL
                     CRLLQMGCLESVRILGVGRSPMTEEEFQMVVHEALQSSKKTEHLEEHHWQKLAERLHY
                     LAGELDDENTYRHVSDRLTELAAAGASSNRLFYLATPPSLFSTIVKRLGEAGLNNEEK
                     YWSRIVIEKPFGRDLESAKALNATVAEVFEESQVYRIDHYLGKDTVQNILVFRFGNSM
                     FEPIWNRNYVDYVEITAAEQGGVGSRAGYYEEAGALRDMVANHLLQLLTLTAMEPPIA
                     FEADSVREEKVQVLRSIRRLKPEQVAERTVRAQYGPGEIEGEKVKGYKEEEGVAGSSV
                     TETYAAVEFVISNWRWSGVPFYVRTGKHLGRNLTEIAVHLKRTPQALFARTPTDEIE"
BASE COUNT          565 a          613 c          739 g          530 t
ORIGIN      
        1 agcttgaact gctctgcggt cggccagtaa acgctgtcgg cgtcaaacgc ataggtgaga
       61 tcgataatgc gatcgggcgg aagtatgggc gatttctgcg ccatcgccgg aatcataagc
      121 accaataaaa tgacaagcag ctgcaaacac ctcatctgga gtgctcctta gcgtagggtt
      181 tctgttatct tcaggtaaac aaggacacat aggcaaggag cttttaatgc aaatcggaat
      241 gattggctta ggacgcatgg gcgcgaacat ggtgcgacgc cttttgcgcg gggggcacga
      301 atgcgtggtc aacgatcgca gcgctgacgc cgttgaagca ctgctggctg aaggcgccgc
      361 cggcgccacg tcgctcgacg gttttatcgc aaaactcaaa tcgccgcgca cggtctggtt
      421 gatgataccc gcggcgcttg tcgataccat tcttaacgat cttacgagtg tgttatccgc
      481 cggtgacatc atcatcgacg gcggcaattc ttattacatc gacgacattc gccgcgcgcg
      541 cgaactcaaa gcgaaaggca ttcattacgt cgacgtggga acgagtggag gagtttgggg
      601 actcgatcgt ggttactgcc agatgattgg cggtgaaaag gagatagtca cttatctcga
      661 tcccatcttc aaaactctcg cgcccggaag tggctcaatt tcgccgacgc ccggtcgagc
      721 agccgacgcg ggaacagccg gcgaaggtta cctgcattgt ggtccatcag gcgccggcca
      781 cttcgtgaag atggttcata acggcatcga gtatggattg atggctgcat atgccgaagg
      841 gttgaacgtt ttgcatcatg cgaatgaagg gaagggcgaa agagctgcgg acgccgagac
      901 aacgccgctg cgctatcccg atcattatca atacgatttc aacctggccg acattactga
      961 ggtgtggcga cgcggcagcg tcatcgcttc gtggttgttg gatcttactg cgattgcttt
     1021 cgctgaatcg ccgcagttgg aaggattttc cgggcgtgtt tccgactccg gcgagggacg
     1081 ttggaccatc aacgcggcga ttgaagaagg cgtgccggtg ccggtgttga gtgcggcgtt
     1141 gttccagcga ttcagctcgc gcggcgaggc tgatttcgct aataagattc tgtctgcgat
     1201 gcgattgcaa tttggcgggc acgtggagcg gaagtaaagt ttcacaggat taagcaggat
     1261 tcacggggat tactatgtca gcaatagttg tagaaccaag cccagttcag tatgagcaac
     1321 caacactgcc gcaagccgag ccgtgtgtgg ttgtgatctt tggcgccact ggagatctaa
     1381 ccaaacgaaa gctcatgccg gcgttgtgcc gtctgttaca gatggggtgt ttggagagcg
     1441 tgcgaattct cggcgtcggc cgcagtccga tgactgaaga agagtttcaa atggtcgtgc
     1501 acgaagcgct tcaaagctcg aagaagacag agcatctcga agagcatcac tggcaaaaac
     1561 tcgccgaacg tcttcattac ctggccggtg agctcgacga cgaaaacacg taccgtcacg
     1621 tttccgatcg tctcactgaa ctggctgccg caggcgcgag cagtaatcgt ctgttttatc
     1681 tggccacacc gccgtcgctg ttctcaacca tcgtgaagcg actcggcgaa gccggactta
     1741 ataacgaaga aaagtactgg agtcgcatcg ttatcgaaaa acctttcggg cgcgatcttg
     1801 aatcggcgaa ggcgctgaac gcgactgtgg ccgaagtctt cgaggagagc caggtttatc
     1861 gcatcgatca ctacctcggc aaagacacgg tgcaaaacat tctcgtgttt cgttttggga
     1921 actcgatgtt cgaaccgatc tggaatcgta attacgtcga ctatgttgag atcaccgcag
     1981 ccgaacaggg tggcgtcggc agtcgcgctg gttactacga agaggccggt gccctgcgcg
     2041 acatggttgc taatcatctg ctgcaactgc taacgctaac tgcgatggag ccgccgatag
     2101 cgttcgaagc cgattcggtt cgcgaagaga aggtacaagt gctgcgatcg attcgtcgcc
     2161 tgaagccgga gcaggtcgcc gagcgcacgg tgcgcgcgca atatggacca ggcgagatcg
     2221 aaggcgagaa agttaaaggc tacaaggaag aggaaggtgt cgctgggagt tcagtgactg
     2281 agacgtacgc ggcggtcgag tttgtgatct caaactggcg ctggtcgggt gtgccgtttt
     2341 acgtgcggac gggaaaacat ctcggccgca acctgaccga gattgcagtg caccttaaac
     2401 gcacgccgca ggctctgttt gctcgtacgc cgactgacga gatcgag
//
LOCUS       DASSFQ010000041         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_317345, whole genome shotgun sequence.
ACCESSION   DASSFQ010000041 DASSFQ010000000
VERSION     DASSFQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317345"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFH96_01415"
     CDS             complement(<1..269)
                     /locus_tag="VFH96_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972648.1"
                     /translation="MKKRYLIAGTATAAATIAVAAKLLLRPRDADWLRYRHVVFHSDH
                     SRFVEIDGMRVHFQEAGEKDNPALVLIHGFASSTLVWSKVFLNLA"
     gene            complement(342..2279)
                     /locus_tag="VFH96_01420"
     CDS             complement(342..2279)
                     /locus_tag="VFH96_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HET6972649.1"
                     /translation="MQEQDGNKGFNISERLEDLRREHEALRWEGTFRDYFELVAQNPR
                     IAQLSHARINDMVHAAGVERLNEGTQHEVLSYNFFAPELFGIEEPIARIVEYFKSAAQ
                     RLEVRKRILLLMGPVGGGKSTIVNMLKRGLEDWTRTQGGSVYAIKDCPMHEEPLHLIP
                     HQLRAEIEKHYGIYIEGDLCPQCRYSLDRTYGGRHEDVKVHRVVFSEKERVGIGTFSP
                     SDPKSQDITELTGSIDLSTIGEVGVESDPRAYRFDGELNIANRGLMEFVEMLKVDEKF
                     LYSLLTLSQEQSIKTGRFAMIYADEVILSHSNENEYLAFAGNRKSEALQDRIILVRVP
                     YNLRVSQEERIYDKLLHQSEALRNVHLAPNTLRVAAMFAVMTRLEEPKRQNVDIVKKM
                     KLYDGEDVEGYKSKDVRELKEETIREGMDGISPRYIINRLSSALVRDGVTCINPIDAL
                     RAIKDGFEQHTGISAEQRERYLNHISQARKEYDELAKIEVQRAFVYSFEEMARTMCNN
                     YLDNVEAFCNKERIKDPITEEEMDPDEQLMRSIEEQIGISENAKNTFRQEILIRISSY
                     ARKGKPFEYNSHERLKEAIEKKIFADLKDVVKITTSAKTPDPDQLRRINEVVDRLVKE
                     HGYCPVCANELLTYVGTLLSR"
BASE COUNT          523 a          729 c          713 g          634 t
ORIGIN      
        1 tgggccaggt tgagaaacac cttgctccac acgagcgtcg acgaggcgaa gccgtgaatc
       61 agcaccagcg ccgggttatc tttttcaccc gcctcctgga aatgaacgcg catcccgtcg
      121 atctcgacga agcgcgaatg atccgaatga aaaacaacat ggcgataacg tagccagtca
      181 gcgtcgcgcg gtcgcagcag aagcttggca gcgactgcga tggtggcagc agccgttgcc
      241 gttccggcga tcaagtaacg tttcttcatc tcccatatca taacgcccgc gtccgaagag
      301 gagcgcgggc ggtatgtgtc tatacgttat ccgtgtcctg cctaccgcga caacagcgtg
      361 ccgacatagg tcagcaattc gttagcgcag accggacagt acccgtgctc cttaacgagg
      421 cggtccacca cctcgttgat tctccgcaac tgatccggat cgggcgtctt cgcggaggtc
      481 gtaatcttca ccacgtcttt caagtcggcg aagatcttct tctcgatcgc ttctttcagg
      541 cgctcgtgcg agttgtattc aaacggcttg cctttgcgcg cgtaagacga gatgcgaatg
      601 agtatctcct ggcggaacgt gttcttggca ttctcggaga taccgatctg ctcctcgatc
      661 gagcgcatca actgttcgtc cggatccatc tcctcttcag taatcggatc cttgatgcgt
      721 tccttgttgc agaaggcttc gacgttgtcg agatagttgt tgcacatcgt gcgggccatc
      781 tcttcaaacg agtagacgaa cgcgcgctgc acttcgatct tcgcgagctc gtcgtattct
      841 ttgcgcgcct gcgaaatgtg attcaagtat cgttcgcgct gttcggcgct gatgccggtg
      901 tgttgttcga agccgtcttt gatcgcccgc aacgcgtcga tcgggttgat acaagtcaca
      961 ccgtcgcgca ccaacgcgct cgacagtcga ttgataatgt aacgcggcga gattccatcc
     1021 atgccttcgc gaatggtctc ttctttcaac tcgcgcacgt ctttcgactt gtaaccttca
     1081 acgtcctcac catcgtaaag cttcatcttc ttcacgatgt cgacgttttg tcgcttcggt
     1141 tcctcgagcc gcgtcatcac ggcgaacatc gccgcaactc tcaaggtgtt tggcgccaga
     1201 tgcacattgc gcagtgcttc cgactggtgc aacagcttgt cgtaaattct ctcttcctgg
     1261 gaaacgcgaa ggttgtaggg gaccctcacg agaataatgc gatcctgcag cgcttcagac
     1321 ttgcgattgc cggcgaacgc gaggtactcg ttctcgtttg agtgcgaaag gatgacttcg
     1381 tccgcgtaga tcatcgcgaa gcggccggtc ttgatgctct gttcctgcga aagcgtcaaa
     1441 aggctgtaga gaaacttctc gtcaaccttc aacatctcga cgaattccat caagccgcgg
     1501 ttggcgatgt tcagctcacc gtcaaatcga tacgcgcgcg gatctgattc gactcccacc
     1561 tcgccgattg tcgatagatc gatcgagccg gtgagctccg tgatgtcctg cgacttggga
     1621 tcgctcggcg agaacgtgcc gatgccgacc cgctcttttt ccgagaacac cacacgatgc
     1681 actttcacgt cttcgtggcg gccgccgtaa gttctatcga gtgaatagcg gcactgcgga
     1741 cacaggtcac cttcgatgta gatgccgtaa tgtttttcga tttcggcgcg cagttggtgc
     1801 gggatcagat gaaggggttc ttcgtgcatc gggcagtctt tgatggcgta gactgagccg
     1861 ccctgcgtcc tggtccaatc ttccagtccg cgcttgagca tgttgacgat cgtggattta
     1921 ccgccaccga ccggacccat cagcaaaaga atgcgtttgc gcacttccaa acgctgcgcg
     1981 gccgatttga agtactcgac aattctggcg atcggttctt cgatgccaaa gagctcggga
     2041 gcaaagaaat tgtaggaaag gacttcgtgc tgcgtgccct cgttgagccg ctccacaccg
     2101 gcggcgtgca ccatgtcgtt gatgcgcgcg tgcgagagtt gcgcgatgcg tgggttctgg
     2161 gcgactagtt cgaagtaatc tcggaaggtc ccttcccagc ggagcgcctc gtgttcgcgg
     2221 cgcaaatctt ccaagcgttc actgatgttg aatcctttgt tgccatcctg ctcttgcata
     2281 gccttgaaac ctcttcggct ttcgcgcatt ggacagcgtg tggggagagg gccacgctta
     2341 gtaaagcaac cgcctgcgat cggggatgcg gttggtaaat cattactact tcgtgatacg
     2401 cctcacgcgc gcgtaagcgt gagctgcgtc ctcgaagcat cagttctcaa tgatcgtgtc
     2461 aacggaacta gcggaaggaa ctacttgttg gaagcggcgg tgcgctccct tgggattgca
     2521 gtatagcaca ccggtaaaga ccctaagtca atgttaaatt ttcatgtgag cactacgcgt
     2581 gggtgtaaga gttgcgcac
//
LOCUS       DASSFQ010000042         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_914358, whole genome shotgun sequence.
ACCESSION   DASSFQ010000042 DASSFQ010000000
VERSION     DASSFQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914358"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2214)
                     /locus_tag="VFH96_01425"
     CDS             complement(<1..2214)
                     /locus_tag="VFH96_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HET6972650.1"
                     /translation="MQDFEKLGVFYLGKNKSNDDLLLYDSKDLVTHAVCVGMTGSGKT
                     GLCISLLEEAAIDSIPAIIIDPKGDLCNLLLAFPNLAPQDFAPWVEEGVDPAQQATLW
                     KDGLAKWGEDGARIKRLQDAADFRIYTPGSNAGLPVSILKSFAAPPEAIRQDNESLAE
                     RISTTVTSLLALVGIEGDPVRSREHILISNILNQEWSNGKDLDIAGLIQKVQSPSITK
                     IGVMDLDSFVPADDRFQLAMALNNLLASPSFASWMEGEALDIAKFLHTNGGKPRHSIF
                     SIAHLGDAERMFFVSLLLNQVLGWMRTQSGTSSLRAILYMDEIFGYFPPVSNPPSKLP
                     LLTLLKQGRAFGLGVVLATQNPVDLDYKGLANTGTWFIGRLQTERDKARVLEGLEGIA
                     AGTGQKFDKQAMEQLLAGLDKRIFLLNNVHDDGPELFETRWALTYLRGPLTRAQIKLL
                     MDPLKQGGTPAPAPVASTAVPAAAPAPVASALAKNERALLPPDITQYFIPVRGSGDAN
                     SVLTYHPTLLGAAEVRYSDSKSVDMVQQVTLLATFGDGPVSLDWDTAVPVDLPVEDLE
                     SDAQAGAQFGEVPSDATKAKSYASWRKDLSGWLYRNQRLELFESPTLDIVSNPGESER
                     DFRVRLQQLGREQRDDAVEKLRRKYAPKLEQLEERKRRAEQAVAREAEQAKGQKMQTA
                     ISFGATLLSSFLGRKRTSLSTLGRATTAVRGVGRSMKESQDVDRAEETVSVISQKL"
     gene            complement(2297..3115)
                     /locus_tag="VFH96_01430"
     CDS             complement(2297..3115)
                     /locus_tag="VFH96_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6972651.1"
                     /translation="MNSIYPFADIELARRLERTEAQGNVDFIEARKKAFPERDATWIE
                     IAGTFAMFDGVGSPLTQTFGLGVSQPITTEQIETIEDFFQSRGAEVFHEVCPLADPSV
                     FDLIRERDYRPVEFSNVLYRPIDPELRLEAPRNEQIEVRVIEKNDIKIWSQTACEGWS
                     EFPEVADFLRELNQVYAHSDGPSFLAELKGAPIATGALTIAGDVALLAGASTVPTARR
                     QGAQLALLEERLRYAAAHGCTVAMMAAQPGSGSQRNAERHGFRIAYTRTKWSKS"
BASE COUNT          667 a          917 c          869 g          727 t
ORIGIN      
        1 gagcttctgc gagatcacgg acacagtctc ctccgcgcga tcgacgtcct gcgattcctt
       61 catcgatcga ccgacgcccc ggactgcggt tgtggctctg ccaagtgtcg aaaggctcgt
      121 tcgcttccga ccgaggaaag aagaaagcaa cgttgcgccg aacgagattg cagtctgcat
      181 cttctgccct ttcgcctgct cggcctcgcg cgctacagcc tgttcggcgc gacgttttcg
      241 ttcttccagc tgttctaact tcggcgcgta cttgcgtcgc agcttctcaa ccgcgtcatc
      301 gcgttgctct ctgccgagct gctgcaaacg gacgcgaaag tcgcgctcgc tctcgccggg
      361 attcgaaacg atgtcgagcg ttggactctc aaacaattcc aaacgctggt ttcgataaag
      421 ccagcccgat agatccttac gccagctcgc gtaactcttc gccttcgtcg catcactcgg
      481 cacttcacca aactgtgctc ccgcctgtgc gtccgactcg aggtcttcga cgggtaaatc
      541 cactggcacg gccgtgtccc agtcgagact aactggccca tcaccgaacg tggcgagcaa
      601 agttacctgt tggaccatat cgactgactt cgaatcgctg taacgaactt cagcggcgcc
      661 gagcagggtt gggtgatatg tcaggactga attggcatcg ccggatccgc gcacgggaat
      721 aaagtattga gttatatccg gcggcagcag cgcgcgctcg ttttttgcca gtgcgctcgc
      781 aaccggcgcc ggcgcagcgg caggaacagc agtgctggcg accggagccg gtgctggcgt
      841 tccaccctgc ttgagaggat ccatcagcaa cttgatctgc gcgcgtgtca atggaccacg
      901 caggtatgtg agtgcccagc gcgtttcgaa gagttcaggt ccatcgtcgt gcacgttgtt
      961 tagcaggaag atgcgcttat cgagtcccgc gagcagttgt tccatcgcct gcttgtcaaa
     1021 cttctggccc gtgccggccg cgatgccttc gagtccttcg agcacgcgcg ctttatcacg
     1081 ttccgtttgc aggcgaccga taaaccacgt accggtgttc gcgagtcctt tgtaatcgag
     1141 gtcgaccgga ttctgtgttg cgagcaccac gccgagacca aacgcgcgtc cctgtttcag
     1201 cagcgtcagc agcggcaact tcgacggcgg attcgaaacc ggcggaaagt aaccaaagat
     1261 ctcgtccatg tagagaatcg cgcgcaggct ggacgtgccc gactgtgttc tcatccaacc
     1321 gagcacctga ttcaggagca acgacacgaa gaacatgcgt tcagcatcgc cgagatgtgc
     1381 gatcgagaaa atcgagtgac gcggtttgcc tccgttcgtg tgcagaaact tcgcaatgtc
     1441 gagcgcttcg ccttccatcc aactggcaaa actcggcgac gcgagtagat tgttgagcgc
     1501 catcgcgagt tgaaagcgat cgtccgccgg gacaaacgaa tcaagatcca tcaccccgat
     1561 ctttgtgatc gatggtgatt gaactttctg aatcagaccg gcgatgtcga gatcttttcc
     1621 gttggaccac tcctggttga gaatgtttga gatcaggatg tgttcgcgac tgcggaccgg
     1681 atcgccttcg ataccgacaa gtgctaatag actcgtaaca gtcgtgctga ttcgttctgc
     1741 gagtgattcg ttatcctgcc tgattgcttc gggcggcgcg gcgaatgact tgagaatgga
     1801 gacaggcaag cccgcgttgc tgccgggcgt gtaaatgcga aagtcggccg catcctgaag
     1861 acgcttgatg cgcgcgccgt cctctcccca ctttgcgaga ccatctttcc agagagtcgc
     1921 ttgttgcgcc ggatcgacac cttcttcaac ccacggcgcg aagtcctgtg gcgcgagatt
     1981 gggaaaagcg agcagcaagt tacaaagatc gcctttggga tcgatgatga ttgccgggat
     2041 gctgtcgatt gcagcttctt cgagtaacga aatgcagagg ccggttttgc cactgccggt
     2101 catgccgacg cagacggcgt gtgtgacgag atctttcgaa tcgtagagca gcagatcgtc
     2161 gtttgattta tttttgccga gatagaagac gccgagcttt tcaaagtcct gcatgattcc
     2221 ccctatcctg aataatcttg ttaatcctgt ctaaattagt ctccaagttt tagctggaca
     2281 ggatttacaa gattgttcag gatttgctcc atttggtgcg agtgtatgcg atccggaagc
     2341 cgtgacgctc cgcatttctc tgcgaaccgc taccgggttg cgcggccatc atcgcgactg
     2401 tgcagccgtg cgctgcagcg tagcgcagtc gttcctcgag cagcgcaagt tgagcgcctt
     2461 gtctacgcgc agtcggaacg gtgctcgcgc ccgccagtaa cgcaacgtca cccgcaatcg
     2521 tgagcgcgcc ggttgcgatt ggcgcccctt ttaactcggc aagaaacgat ggaccgtccg
     2581 agtgagcata aacctggttt agttcgcgca ggaagtcagc aacttctgga aactcactcc
     2641 agccttcaca cgctgtctgc gaccagatct taatgtcatt tttctcgatg actcgaactt
     2701 cgatttgttc gtttcgcggc gcttcgagac gtagctcagg atcaatgggc cggtacagca
     2761 cgttcgaaaa ctcaacgggt cgatagtcac gctctcttat cagatcaaac accgacgggt
     2821 ccgcgagcgg acaaacctca tgaaaaactt ccgcgcctcg cgactgaaag aaatcctcga
     2881 tcgtttcgat ttgctccgtc gttattggtt gagaaacacc gagtccaaac gtctgtgtca
     2941 acggtgaacc aacgccatca aacatcgcaa acgtgcccgc gatctcgatc cacgtcgcat
     3001 cacgctccgg aaacgccttt tttcgtgcct cgataaaatc gacgttgccc tgcgcctcag
     3061 tccgttccag ccggcgcgcc agctcgatat ccgcgaacgg ataaatacta ttcatcgtgg
     3121 aagctttgcc tcaaggccgg gaaatcattc ttggatcggg tcagaacagg gagcggtagc
//
LOCUS       DASSFQ010000043         4602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1958327, whole genome shotgun sequence.
ACCESSION   DASSFQ010000043 DASSFQ010000000
VERSION     DASSFQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958327"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFH96_01435"
     CDS             <1..428
                     /locus_tag="VFH96_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6972652.1"
                     /translation="DQAVIPEKVMVAIASHGSAKKLLRTGSRIAGRLASDWYAVYVET
                     PREEMGRIRPEDYAALQENIQFAEQRGARVVRLKARSVADALIDFARREGITHVIFGQ
                     TSRTRWDIILHGSVINRFLDEVRDATVQVVPLDKRTNES"
     gene            418..2115
                     /locus_tag="VFH96_01440"
     CDS             418..2115
                     /locus_tag="VFH96_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6972653.1"
                     /translation="MSLRSKLLTGYAVFIAALVVLGAWSAWRLHEMGKVSRRIISENY
                     DSVIAAQEMKESLDLHDRARFDQNLQKAANNITEPGEAEAIAAIRHDRDIYYQRTDAV
                     TENQLRADCDHLLQLNQRAMLLKAETAAGVAQLWFYRTLLIAGILVAAGVFLAFVLAR
                     RIVGPLQRLTESTARIASGDLNARVTVDSTDEVGVLAAEYNRMADNLRQLRNSDMGKL
                     LVAQQTTEAAIDSLFDPVIVTDAEGRVTKLNPAAEEIFGSENENRGMHVGEVARDVRI
                     AGAVAETLESQRPVAGDGMSAFLPLAVAGSEKAFRLRTTPMRDNDHHLLGAVTLLEDV
                     THLREIDRLKSEFIATASHELRTPLTSVQMGVYLLLERAAGELTDKQVEILSACRDDC
                     ERLDKLMRDLLDLSRIEAGESKPNFESIRTRELINDATKELAPQVESKGLGFRVEAPV
                     DLPTVKVDRSQVERVLANLVVNALRYTKQGEIKISAQPRGNSVAVSVSDTGSGIPPEY
                     LPHIFDKFVQVPGAATGGAGLGLAISRLIVEAHGGQISVQSEPDKGSTFTFTLPIAA"
     gene            2221..2955
                     /locus_tag="VFH96_01445"
     CDS             2221..2955
                     /locus_tag="VFH96_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6972654.1"
                     /translation="MNERILVIDDEENIRRMMRLTLEAAGYHVGEAKDGADGLILYAD
                     GSRWDVVILDQRMPGMDGLETLRQLKSQNSHARVVMATAYASIELAVDAMKFGATDFV
                     RKPMTPKVLRNAISAALSKSTASAAPTTKPPQPLIETITMNGYTIIDDDETLALDERQ
                     RRFIVISPDRQKHDVLVQIDPEVIDYVARMIGRRLSAANTFWTAQARRLLSDFLWDER
                     GLPPMKRLVLKNLDPDRLPVAASWKG"
     gene            2971..>4602
                     /locus_tag="VFH96_01450"
     CDS             2971..>4602
                     /locus_tag="VFH96_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6972655.1"
                     /translation="MATAIKRLLVGRALRTEQAAHERLTKKTALAVFSSDALSSTAYA
                     TEEILLVLAAAAAATASGSFNYVVPVSIGIAVLLAIVATSYRQTIHAYPSGGGAYIVA
                     KENLGTTPGLIAGASLLVDYVLTVSVSIAAGVAAITSAVQGPRYAGLSEHKVILCLIF
                     IAFIATANLRGVRESGALFAAPTYVFVVSFLFMIVFGLFHYFIYGGAAPVPSAEELKI
                     AEGYRLQPMTLFLVLAAFSNGCAALTGIEAISNGVQAFKKPEAHNAATTLIWMAILLT
                     AMFLGTSVMAFLYHVHPKESETVISQFARIMFTGPLGWFYYVVQAATAAILVLAANTA
                     FADFPRLSSLLARDRFLPRQFANRGDRLVFSNGIVILAIFSGVLVTAFGGDTSRLIPL
                     YAVGVFLSFTLSQSGMVVHWLRVRRTSPKPVAKEEEIHRSYFVTDEVTAPSNWKKSLA
                     INAVGALATFVVLCVFIATKFIHGAWIVVVVIPLLVLLFRAIHKHYLMVAKQLSTEGL
                     EPLRPIKHTVIVPISGIHRGVVSALQYARSIASDRV"
BASE COUNT          968 a         1249 c         1386 g          999 t
ORIGIN      
        1 tggaccaggc cgtgattccg gaaaaagtga tggtcgcgat tgcttcgcat ggtagcgcga
       61 agaaattgtt gcgcaccggc tcgcgaatcg ccgggcgact tgcgtcggat tggtacgcgg
      121 tctatgtcga gacgccgcgc gaggagatgg ggcgaatcag gcctgaagac tacgccgcgt
      181 tgcaggagaa cattcagttt gcggaacagc gtggggcgcg cgtggtgaga ctcaaagcgc
      241 gcagtgtggc agatgcgctg atcgatttcg cccggcgtga agggatcacg cacgtgatct
      301 tcggacaaac gtcgcgcaca cgctgggaca tcatcctgca cggctctgtg atcaatcgct
      361 ttctcgacga ggtccgcgac gcgactgtgc aagttgtgcc cctggataag agaacaaatg
      421 agtcttagat ccaagttgct gacagggtac gcagttttta ttgccgcgtt ggtggtgttg
      481 ggggcgtgga gtgcgtggcg gctgcatgag atggggaagg tgtcgcggcg cattatctct
      541 gaaaattacg actccgtaat tgcggcgcag gagatgaagg agagtctcga cctccacgat
      601 cgcgcacgct tcgatcagaa cctgcaaaaa gccgcgaaca acattactga gcccggtgag
      661 gctgaagcaa tcgccgccat ccgccacgat cgagacattt attaccagcg cacagatgcc
      721 gtcaccgaga atcaactacg cgcggactgt gatcatctgc tgcagctcaa tcaacgcgcc
      781 atgctgctca aagccgaaac cgcggcaggt gtggcccaac tctggttcta tcgcacgctt
      841 ctgatcgccg gaatcctcgt ggccgccggt gtatttctcg cgttcgtgct ggcgcgaagg
      901 attgttggac cactgcaacg tctcacggaa agtacggcca ggattgcgag tggagatctc
      961 aacgctcgag taacagtcga ttcgactgac gaagtaggcg tgcttgctgc ggaatacaac
     1021 cgcatggccg acaatttgcg acaactgcgc aattccgaca tgggtaagct actcgtggcc
     1081 cagcaaacta ccgaagcggc gatcgactcg ctcttcgatc ccgtgatcgt aaccgacgct
     1141 gaaggccgcg taaccaagct caatcctgcc gccgaggaaa tattcggttc tgaaaacgag
     1201 aaccgcggga tgcatgtcgg cgaagtagcg cgcgacgtgc gtatcgccgg tgcagttgcg
     1261 gagacacttg aatcgcagcg tcccgttgcc ggcgacggca tgtcggcgtt cttaccgctc
     1321 gcagtcgcag ggtcggaaaa agcgtttcga ctgcgcacga cgccgatgcg cgataacgat
     1381 caccacctgc tcggcgcggt caccctgctg gaagacgtca cacatctacg cgaaatcgat
     1441 cgactcaagt cggagtttat cgccaccgct tcgcacgagt tgcgcacgcc gctgacgagc
     1501 gtgcagatgg gtgtctatct actgctcgag cgcgcggctg gagaacttac ggacaaacag
     1561 gtcgagatct tgagcgcttg tcgcgacgac tgcgaacgtc tcgacaagtt gatgcgcgac
     1621 ttgcttgacc tctcgcgcat cgaggcaggc gaaagcaagc caaacttcga gtcgatccga
     1681 acgagagagt tgattaacga cgccacgaag gaattggcgc cgcaggttga gtctaaaggc
     1741 ttgggtttca gggttgaggc gccggtcgat ttgccgacgg tcaaggtcga tcgatcgcag
     1801 gtggaacgcg tgttggccaa cctcgtcgtg aatgcgttgc gttacacaaa acagggcgag
     1861 atcaagatca gcgcgcagcc gcgtggaaat tcggttgccg tttcagtgtc tgacacgggt
     1921 agcggaattc cgccggagta tctgccgcat atcttcgaca agtttgtgca ggtacctggt
     1981 gctgcgactg gtggcgctgg attgggtttg gccatttcga gattgatcgt cgaagcacac
     2041 ggcggacaga taagtgtgca gtcggaaccg gacaaaggtt cgacgttcac atttaccttg
     2101 ccgatcgccg cgtgatttgg gcgactcctt ggagtgcgcc gacttgtcgg cgctttagta
     2161 ctgataccaa agcggtgcca aggcaccgca ctccaaagtg gcgctcgatg ttagagagtt
     2221 atgaacgaac gaatactggt catcgatgac gaagagaaca ttcgccgcat gatgcggctg
     2281 acgctcgaag ccgcaggcta tcacgtgggc gaggcgaagg acggcgcgga tgggctgatt
     2341 ctctatgctg atggatcgcg ttgggatgtc gtcattctgg accagcgaat gccgggaatg
     2401 gatggcctcg aaacgctgcg acaactcaag agtcagaact cccacgcgcg tgtagtgatg
     2461 gcgacagcct acgcctcaat cgagcttgcc gtcgatgcga tgaaattcgg cgccactgac
     2521 tttgtgcgca agccgatgac gccgaaagtc ctgcgcaacg cgattagtgc cgccttatcg
     2581 aagtcaaccg cctcggccgc tccgaccacg aagccaccgc agccgcttat cgaaaccatc
     2641 acgatgaacg gctacacgat catcgacgac gacgaaacac tcgcgctcga cgagcggcag
     2701 cgtcgcttca tcgtgatcag tccggatagg cagaagcacg acgtcctggt ccaaatcgat
     2761 ccggaagtaa tcgactacgt cgcgcgcatg atcggccggc gactgtctgc cgctaacacg
     2821 ttttggacgg cacaagcgcg tcgcctgctg agtgacttcc tgtgggatga acgcggcctg
     2881 ccgccgatga agcgtctcgt tctgaagaat ctcgatccag acaggttgcc ggtggcggca
     2941 agttggaagg gctgaagggg taaacgttaa atggcaactg cgatcaagcg gttactggtt
     3001 ggaagagcac tgcgaaccga acaagccgcg cacgagcggc tcacaaagaa aactgcgctc
     3061 gccgtttttt catccgacgc gctttcgtcg accgcatatg caaccgaaga aatcctgctc
     3121 gtccttgccg ccgcggcggc tgcgactgcg agtggatcgt tcaactacgt ggtgccggtt
     3181 tcgattggaa tcgccgtgtt gctggcgatt gtcgcgacga gctaccggca aacgattcat
     3241 gcgtatccgt caggcggcgg cgcttacatc gttgccaaag agaatctcgg gacgactccg
     3301 ggactgattg ccggcgcgtc gctactggtg gattacgttc tgactgtgtc ggtgtcaatc
     3361 gccgccggcg tggctgcaat cacatcggcg gtgcagggac cacgttacgc cgggctcagc
     3421 gagcacaaag taatactgtg tttgatcttt atcgctttca ttgcgactgc aaacctgcgc
     3481 ggtgtgcgcg aatcaggggc gctcttcgcg gccccaacgt atgtcttcgt ggtcagcttc
     3541 ctgttcatga ttgtcttcgg tctctttcat tacttcattt acggcggtgc tgctccggtc
     3601 cccagcgccg aagagttgaa gattgctgaa ggctaccgac tgcaaccgat gacactgttt
     3661 ctcgtgctcg cagcattctc aaacggctgc gccgcgctca ccggcatcga agcaatctcg
     3721 aacggcgtgc aggcattcaa gaagccggaa gcacataacg ccgcgacgac gttgatctgg
     3781 atggcgatac tgctgacggc gatgttcctt ggcacgagcg taatggcttt cttgtatcac
     3841 gtgcatccga aagaaagtga gacggtgatt tcgcagtttg cgcgcatcat gttcactgga
     3901 ccactaggat ggttttacta cgtggtgcag gctgcgacgg ctgcgattct cgtgctcgca
     3961 gcgaataccg catttgccga ttttccacgt ctctcgagtt tgctggcgcg cgatcgtttt
     4021 ctgccgcgcc agtttgcgaa tcgtggagac cggctggtct tttcaaatgg cattgtgatt
     4081 ctcgcgatct tctccggcgt gctggtcact gcgtttggtg gcgatacgtc acgattgatt
     4141 ccgctttacg cggtcggcgt gtttctttcg tttacgttgt cgcagtcggg gatggtggtg
     4201 cattggttgc gcgtacgaag aacatcgccc aaaccggtgg ccaaagaaga agaaatacat
     4261 cgatcatatt tcgtcactga tgaagtcact gcgccctcga actggaagaa gtcgctggca
     4321 atcaacgcag tcggcgcgct cgcgacgttt gtcgtgctgt gcgtcttcat tgcgacgaag
     4381 ttcatccacg gcgcctggat tgttgtcgtc gtaattccat tgctcgtgtt gttgttcaga
     4441 gcgattcaca aacactattt gatggttgcg aagcaactct cgactgaagg attggagccg
     4501 ctgcgaccga tcaagcacac ggtcatcgtc ccgatttccg ggatacatcg cggcgttgtt
     4561 tcggcgcttc agtacgcgcg ctcaatcgct tcggacagag tg
//
LOCUS       DASSFQ010000044         5989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2104028, whole genome shotgun sequence.
ACCESSION   DASSFQ010000044 DASSFQ010000000
VERSION     DASSFQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104028"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..501)
                     /locus_tag="VFH96_01455"
     CDS             complement(97..501)
                     /locus_tag="VFH96_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6972656.1"
                     /translation="MKPRILIVDDDSQITQQLYWTLCDEYDVVTANDLQTAIRRATFY
                     KPDISILDLQMPPEKDSTASGLRLLEYIKGHLANSRVLIMSSNATTETRRACFAAGAD
                     GFFAKPFEIQDMLASICKMSPLHRLELFAHIL"
     gene            complement(655..1713)
                     /locus_tag="VFH96_01460"
     CDS             complement(655..1713)
                     /locus_tag="VFH96_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972657.1"
                     /translation="MPARAQDKPLTNQEFVGLLYQLQRNPSVRDDLIEQIRTRGIGFP
                     LTDGMRGLVATKSGNDAVLRRTLEEAERRRVNPTASKLPPETEGKELLERTRNVTLAA
                     ANAMPDFIVKQIIRRSVAYGNTANWIPQDNLTIAVGYRANVGEEYKVLTVNGMAPSED
                     EGSSKDYSRNKSVPKGASSSGVEYISAQADIFKPESQTEFRMVDTDVILGKRTVVYEY
                     TVKRENSQLTLGLADTGARATVGSRGRLWIDRELDRVVRFEQIATEIPPDFPITAASS
                     LIDYDWVSINERKYLLPTHSEILLTTVNPKFVLQSRNDIKFRSYQKFGAELKVVDEVG
                     EDDDEPPPVPKPIKPPQN"
     gene            complement(1912..3018)
                     /gene="argE"
                     /locus_tag="VFH96_01465"
     CDS             complement(1912..3018)
                     /gene="argE"
                     /locus_tag="VFH96_01465"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HET6972658.1"
                     /translation="MIKTILADLVRIDSVSARSNVEIVEYLEGRCQALGLITSRLPYT
                     DQHGVQKYNLIALSHPTSDVELALVGHTDTVPYDPNWSEAINLTERDGKLYGRGSCDT
                     KGFIAAALTALETTKPQKPMALVFTADEEIGLVGAKMLAESQPLRVRYSIVGEPTSLQ
                     PIRAGKGYSLAEVIVKGREAHSAYPALGASAVMRAARLINRLEAIANELKNDQHPAFD
                     PPFTTLNIGLIHGGSAKNVLAGECRFTLEWRPIPTQSSEHLLNLFQEAIEEEKKLDPD
                     FQCEVDASRQDTGFETNAESPLIRLLEDASGKKSGTVAFGTEAAQMKLLGSESVVIGP
                     GDIREAHRTGEFVPVDELERCAEILRQAVEELCN"
     gene            complement(3015..4343)
                     /locus_tag="VFH96_01470"
     CDS             complement(3015..4343)
                     /locus_tag="VFH96_01470"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HET6972659.1"
                     /translation="MPAQSWLPDLIYVDGEFHSDRALVCDGDKIVEIVEAAKAPEAIR
                     LKRRAMLPGMVNSHSHAFQRIIRGRTEHRSQHSTDSFWTWREQMYAAANRLGPEEIYA
                     ASRLAFLEMAQTGITAVGEFHYIHHAPDGSTYDDPNLLAREVIRAARDVGIRIALLRV
                     AYARAGFETDPNPQQIRFIEASPDTYLKHLDQLLNAPELKDAFAWAGVAPHSVRAVPL
                     DYLKTIIQFAKDRDLPTHMHVAEQPAEVSACIKEYGRSPVALLDTEGLLSNRFTAVHA
                     IHVTPKAIAAMTRSGAMVCACPTTERNLGDGVVPVDSYFDAGVRVSLGSDSQIQIDLL
                     EDARELEYHLRLQKTARNVLAPADDTSSSSLARRLFDCATINGAASIGFDGGKLAPGA
                     PADFFTVDLDDLSIAGCAVEDLLANIVFSLSRTAIRDVIVAGKQVRGLDG"
     gene            complement(4432..5205)
                     /locus_tag="VFH96_01475"
     CDS             complement(4432..5205)
                     /locus_tag="VFH96_01475"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HET6972660.1"
                     /translation="MMLKPPLKWAGGKRWLVSHLKPIWEANTNRRYVEPFCGGLAVAL
                     GLQPQQSLLNDVNPHLINFYRQVQSGLRIRVRMSNDETLFYRHRERFNRLIRTGKANT
                     AEAAQLFYYLNRTCFNGLCRFNKSGDFNVPFGTYKAINYATDFSSYVDVFRQWEFSNK
                     DIEALELRPKDFVYADPPYDVEFTTYSAGGFSWNDQVRTARLLAKHRGPVVISNQATA
                     RIIELYEDCGFELSYLDAPRRISCTGDRTAAREVLAVKR"
     gene            complement(5202..>5989)
                     /locus_tag="VFH96_01480"
     CDS             complement(5202..>5989)
                     /locus_tag="VFH96_01480"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6972661.1"
                     /translation="LIKVRNFAHARVLLESLGYEMTPRLTPAQLTSHLNNNCEIQFMR
                     DDWFTVVDLHWDLAPRSFVFDLKADEVMSRLQSVSLAGSTVQTFGAEDLLLYQAMHGA
                     KHLWRRPEWISSLAESIRATPAVDWDTLINRAANAHATRMLALGLRLVEQFSDVPVSI
                     IASIDKDGSMQRMAAGIHAQIFNTFGYADSTETNLYNLRIMDRKRDALVSALRSIFVP
                     TLPDWQALALPSSLHPLYYAYRPLRLSKVYGASLWRKLRPRAS"
BASE COUNT         1336 a         1618 c         1655 g         1380 t
ORIGIN      
        1 acggcagcgg gggccatcga gcacaccgtc tgatgaagag ggtttcaccc acggtgcttg
       61 tacggacgtc aaagggagtg acgtccgaat cctttatcaa agaatgtgag cgaacaattc
      121 cagccgatga agggggctca tcttgcaaat acttgcgagc atgtcctgaa tttcaaacgg
      181 cttggcgaaa aatccatcgg cgccggcggc gaaacaggca cgacgcgttt ccgtcgtggc
      241 gttggagctc attatcaaaa ctctcgagtt cgccagatga cccttgatgt attccagtaa
      301 tcgcagaccg ctggcggtcg aatctttttc cggaggcatt tgcaagtcca atatagaaat
      361 atcgggcttg tagaaagtgg cccgccgaat cgcggtctgc aaatcgtttg ccgttacgac
      421 gtcgtactca tcgcacaacg tccaataaag ctgctgggta atttgtgaat cgtcgtcgac
      481 tatcaagatc cttggcttca tcgcttatca ccgggagcaa agactgtacc gcgcactcga
      541 tcacagtacg agatcagcaa acaaagcgga tttagcgttt tgcaggagat tttgcgtgtc
      601 tacagatgtg gacacgctgt agacattgag ccacagatcc gtgtgatccg tggcttagtt
      661 ttgcggtggc ttgatgggtt tcggaactgg tggtggctcg tcgtcgtctt caccaacttc
      721 gtcgacgacc ttcaactccg cgccaaactt ctgatagctg cgaaacttga tatcgttccg
      781 gctttgaagc acaaacttcg gattgaccgt cgtcagcaga atctcggaat gtgtcggcaa
      841 caaatatttg cgctcgttga tcgacaccca gtcgtagtcg atcaagctgc tcgcagcagt
      901 gatcggaaaa tcgggaggaa tctctgtcgc aatctgttcg aagcggacga cacgatccaa
      961 ctcgcgatcg atccacaggc ggccgcgcga tccgacggtt gcgcgcgcgc cagtgtcagc
     1021 gaggccgagc gtcagttgtg aattctctcg cttgaccgtg tactcgtaaa cgaccgtcct
     1081 tttgccgaga atcacatccg tgtcgaccat ccgaaattcg gtttgtgact ccggcttgaa
     1141 aatgtctgcc tgcgcggaga tgtactcaac gccgctagac gaagcacctt tcggcaccga
     1201 tttgttccgg ctgtaatctt tcgagcttcc ctcatcttcg ctcggcgcca ttccgttaac
     1261 agtcagaacc ttgtattcct cacccacatt cgcgcgatag ccgacggcga tcgtgagatt
     1321 gtcctgcgga atccagttcg ccgtattccc gtaggccaca gagcgccgga tgatctgttt
     1381 gacgataaag tcgggcatcg cattagcggc ggcgagcgtt acgttgcgtg tgcgttcgag
     1441 tagctctttt ccttcggttt ccggaggaag tttggacgcg gttgggttga cgcgacggcg
     1501 ttcggcttct tcgagcgtgc gtcgcagcac tgcgtcgttg ccgctcttgg tcgcaacgag
     1561 cccgcgcatg ccgtcagtca aaggaaaacc gatcccgcgc gtcctgatct gttcgatcaa
     1621 atcgtcgcgc acggatggat tacgctgtag ctgataaagg aggccgacga attcctggtt
     1681 ggttagtggt ttgtcctggg ctcttgcagg catccaccac agcaagaaga ctgtcgcaag
     1741 tatgagtaaa acagttttag tgcaagacat tggtatttcc gatggaattt aagcgctgac
     1801 cgttgcccgg aaaagcatct cacgcaaagg cgcaaagatg caaagcagca agaaagccct
     1861 tagaagcgcg gcagcgcttg cgcctttgcg cctttgcgtg agaaatcctt ttcaattgca
     1921 cagttcttca accgcctgtc tcaaaatctc ggcgcaacgc tcaagttcat ccaccggcac
     1981 aaattctccc gtccggtgtg cttcgcggat gtcaccgggt ccaatcacca ccgattcact
     2041 ccccaagagc ttcatttgcg cagcttcagt tccaaacgcg accgtgcctg acttctttcc
     2101 gctcgcatct tcgagtagcc gaattagcgg cgattctgcg ttggtttcga aacctgtatc
     2161 ctgccggctc gcgtcgactt cgcattgaaa gtcgggatcc aacttttttt cttcttcgat
     2221 cgcttcctgg aagagattga gcaaatgctc ggaagactgg gttggaatcg gacgccattc
     2281 gagcgtgaac cggcactcac cggcgagcac gttcttggcc gaaccaccgt ggatcaaacc
     2341 gatgttcaga gttgtgaagg gtggatcgaa agcgggatgc tgatcatttt tgagttcgtt
     2401 agcgatcgct tcgagtcgat tgatcaaacg cgcggcacgc atgacagccg aagcgccgag
     2461 tgcaggataa gcgctgtgtg cttcgcgtcc tttcacgatc acttcggcga gcgaatagcc
     2521 tttgcccgcg cgaatcggtt gcagcgaagt cggctcgccg acgatgctgt aacgcacgcg
     2581 cagtggctgc gactcggcca acatctttgc gccgacgagt ccgatttcct cgtcggcagt
     2641 gaataccagc gccatcggtt tttgtggttt agtcgtctcc agcgccgtca gtgctgcggc
     2701 gataaaacct ttcgtgtcgc atgagccgcg accgtagagt ttgccatcac gttcggtgag
     2761 attaatggct tcggaccagt tcggatcgta aggaacggtg tcagtgtggc ctacgagcgc
     2821 cagctcgacg tcgctggtgg gatgtgacaa cgcgatcaag ttgtacttct gaaccccatg
     2881 ttgatcggta tacggcaaac gactcgtgat cagtccgagt gcctgacatc ggccttcgag
     2941 atactcaaca atctcaacat tcgatcgcgc cgagaccgaa tcaatccgaa ccaaatccgc
     3001 gagaatcgtt tttatcatcc gtctaatccg cgtacctgtt ttccagcaac tatcacatca
     3061 cgaatcgctg ttcgcgaaag tgaaaagaca atgtttgcga gtaagtcttc aaccgcgcat
     3121 cccgcaatcg agagatcgtc gagatcgacg gtgaagaaat ctgcgggcgc gccgggtgcg
     3181 agtttaccgc cgtcaaaccc gatactcgca gcgccgttga tggtggcaca atcaaacagc
     3241 cggcgcgcca gtgacgagct cgaagtgtcg tcagccggcg cgagcacgtt gcgcgcagtt
     3301 ttttgcagac gcaggtgata ctcgagctcg cgtgcgtcct ccagcagatc gatctgaatt
     3361 tgactgtcgc taccgagcga cacacgcacg cccgcatcga aatacgaatc aaccggaacc
     3421 acaccgtcgc cgagatttcg ctcggtcgtt ggacacgcac ataccatcgc gccgctacgc
     3481 gtcattgcag cgatcgcttt tggcgtaacg tgaattgcat gaacagcagt gaagcgattg
     3541 ctgagcaagc cttcggtgtc gagcagcgcg accggcgagc gaccgtattc tttaatacac
     3601 gcgctgactt cagcgggctg ctcggcgacg tgcatgtgcg tcggcagatc gcgatctttt
     3661 gcgaattgaa tgattgtttt caggtagtcg agcggcaccg cgcgaacact gtggggcgcc
     3721 acgccggccc atgcgaatgc gtcttttagt tcgggtgcgt tcagtagttg atccagatgc
     3781 ttgagatacg tgtcaggcga ggcttcgata aagcgaatct gttgtgggtt gggatcggtt
     3841 tcaaaaccag cacgcgcgta agcgacacga aggagcgcga tgcgaatacc gacgtcacgc
     3901 gcggcgcgta ttacttcacg agcgagtagg tttggatcgt cgtacgtcga accgtcaggg
     3961 gcgtgatgga tatagtgaaa ctcgccgacg gcggtaatgc cagtctgggc catctcgaga
     4021 aacgccaggc gtgaagcggc gtaaatttct tccgggccga gacggttcgc ggcggcgtac
     4081 atctgctcgc gccaggtcca gaaactgtcg gtcgagtgtt gcgaacgatg ctcggtgcgg
     4141 ccacgaatga tgcgctgaaa cgcgtgtgag tgagagttga ccattccagg cagcatggca
     4201 cggcgcttca gacgaatcgc ttcaggcgcc tttgcagctt cgactatctc aacaatcttg
     4261 tcgccgtcgc acacgagtgc ccgatcggag tgaaactctc cgtccacgta gattagatct
     4321 ggtaaccagg attgtgcggg catgatttcc aagactctgt tggataaaga tgaacaagat
     4381 tattcaagat ttacatgtca atcctgaata atctttttca tctttatcta tctacctctt
     4441 gacagcaagc acctcgcggg cagctgtgcg atcgccggtg caactaatcc ttcgcggtgc
     4501 atcaagataa ctcaattcaa agccacaatc ttcatacaac tcaatgatcc gcgcagtcgc
     4561 ctggttcgag atcaccactg gcccacggtg cttagcaagc aatcgcgccg tgcgcacctg
     4621 atcgttccac gaaaaacccc cggcgctgta cgtcgtgaac tctacatcat acggtggatc
     4681 ggcgtagacg aaatctttcg gtcttaactc aagcgcctcg atatccttat tactaaactc
     4741 ccactgcctg aacacgtcca cgtaactcga aaagtccgtc gcgtagttaa tcgccttgta
     4801 cgtcccaaac ggcacattaa aatcgccgct cttattaaaa cgacagagac cattgaagca
     4861 tgtgcggttc agataataga acaactgcgc ggcttccgcg gtgtttgcct tgcccgttcg
     4921 aatcagccgg ttgaagcgct cgcgatgacg ataaaaaagc gtctcgtcgt tgctcatccg
     4981 cacgcgtatt cgcaaaccac tctgaacctg gcgatagaaa tttatcagat gaggattgac
     5041 gtcattcagt aacgactgct gcggttgcag accaagcgca accgcgagtc caccgcaaaa
     5101 cggttccacg tacctgcggt tcgtattcgc ttcccagatt ggtttcaggt gagacacgag
     5161 ccagcgcttg ccgccggccc atttcaaagg cggcttcaac atcatgatgc gcgcggcctg
     5221 agctttcgcc agagtgaagc gccgtagacc tttgataaac gcaacggtcg ataggcgtag
     5281 tagagcggat gcagcgacga tggcagcgcc agtgcctgcc agtcaggcaa agtggggacg
     5341 aagatcgagc gcagcgctga gacgagtgcg tcgcgtttgc ggtccatgat gcgcaggtta
     5401 tagagattgg tttcagttga atcagcgtat ccaaaagtat tgaagatctg cgcgtgaatt
     5461 cccgcggcca tgcgttgcat tgagccgtct ttatcgatag aggcgatgat agagactggg
     5521 acgtcagaga actgttcgac gagtcgtaat ccgagggcga gcattcgtgt cgcgtgcgcg
     5581 ttcgcagcgc gattgatgag tgtgtcccaa tcgacggcgg gagtcgcgcg tattgattcg
     5641 gccagcgaag agatccattc gggacgtcgc cagagatgtt tggcgccatg catcgcctgg
     5701 tacagcagca gatcctcagc gccgaacgtt tgtactgtac tgcctgccag tgagactgat
     5761 tgtagtcgcg acattacttc atccgctttg aggtcgaaga caaaactccg tggcgccaga
     5821 tcccagtgaa gatcgacgac ggtgaaccag tcgtcgcgca tgaactgaat ctcgcagttg
     5881 ttgttcaggt gcgaggtcag ttgcgcgggc gtcagtcgcg gagtcatttc atacccgagc
     5941 gactcgagta atacacgtgc gtgggcaaag ttgcggacct tgatcagca
//
LOCUS       DASSFQ010000045         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_171809, whole genome shotgun sequence.
ACCESSION   DASSFQ010000045 DASSFQ010000000
VERSION     DASSFQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171809"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1907)
                     /locus_tag="VFH96_01485"
     CDS             complement(57..1907)
                     /locus_tag="VFH96_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6972662.1"
                     /translation="MSEENLHFYQFGPFRLDPLKRRLSKDEETLRLTPKAFELLLVLV
                     EEHGRTVEKDELLERVWAGTIVEENNLNQNITALRKSLGDSRQESQYIATIPGVGYRF
                     VAEVRKEPAQRRHDAAEERATRPRPVFRYALLILVPLVLVAIAFAWYRREKPQPAISS
                     IMVLPLENLSGDVAEEYFADGMTDALIGDLAQIGGLHVISRTSSMHYKGTKKSLPQIA
                     GEINVDAVVEGTVQRSGERVVVRAQLIQAATDRHLWVQTYTRDVRNVLDLQSEIAQDV
                     ARQVQIQLTPGEQARFTARRAVQPKAYDSYLQGRYLYWNKRTEENLNKAIGLFQNAIK
                     EDPGYAPAYVGLADCYNGLSVVQISALPPVEGRRRAEEAASKALELDGSLADAYTALG
                     YINHYNWNWTAAEQRFKHALELNPNYANAHNFYASYLMSRGRVDESIAASNRARELDP
                     FSLAISVQRGFLLENARRYDEAIEQLRAVIAMDPNHYQAYWVLGHTYAANKQFDEAVR
                     AAEKAVELSERAPGALGILGLAYGLAGRKTEATRVLNELLELNKNRYVTPAALVNVYV
                     GLGDREQTFVWLEKAYAERSNFIAYLKVFPILDPVRTDRRFADLVGRVGL"
     gene            2057..3325
                     /locus_tag="VFH96_01490"
     CDS             2057..3325
                     /locus_tag="VFH96_01490"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET6972663.1"
                     /translation="MKLKGLKNIFLIFAVLICTQTSAWAAQEDVILQWNRVLMETVRT
                     PGQQPATIMPVRSYAIMHAAMFDAVNSIERSHTSYFVDVPGSPNASIEAAAAQAAHDV
                     LAGLYPTRVAIFNAELAISLQGIEENRAQQGIRVGQIVAETLLAERANDGWNVTPPAY
                     SLPATPGNWQPAPGAATFTHYPSVIPFGITSGLQFKPNAPPALTSAEYANDLNEVKEL
                     GSVSSLTRTADQTKVAQLWAGVNTPTNFLFVWNNVARTVSAARGISTVEKARLFALTN
                     IALHDALQTTFASKFHYGLWRPVTAIRRADEDGNASTAADLTWTSLIASPPYPSYAGN
                     NAAIGTSQATILALFFGRDDIAFQHTWEGAGGATRSYAGFSAMADEEARARVYGGIHF
                     TFDNVAGQSVGRNVANYIFANVMRPRQCVR"
     gene            complement(3545..>3769)
                     /locus_tag="VFH96_01495"
     CDS             complement(3545..>3769)
                     /locus_tag="VFH96_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HET6972664.1"
                     /translation="PGHPDVIAGNVFRDGFMESFNSEMAVKFRKYILTNRLPICNRCC
                     GLYMTNPARKFEQKARRNLGLSNNVTLHYP"
BASE COUNT          819 a         1137 c         1007 g          806 t
ORIGIN      
        1 gtttgtcgcg caatcccgac aagctaaagc atgtcggaca tcaactgcat ccctgtctac
       61 aaaccaaccc gcccaactaa atccgcaaac cgtcgatcag tacgtaccgg atccaggatc
      121 ggaaacactt tcaagtacgc aatgaaattg gaccgctccg cgtacgcttt ctccagccac
      181 acaaacgtct gctcccgatc accaagcccg acatagacgt tgacgagtgc agccggtgtt
      241 acataacgat ttttattgag ctccaacaac tcattcaaaa ccctcgtcgc ctcagttttc
      301 cgtccggcca gaccatacgc gagcccgaga atgccgagtg cgccgggtgc tcgctcagac
      361 aactcaactg ccttctcggc cgccctaacc gcttcgtcaa attgcttgtt tgcagcataa
      421 gtgtgaccga ggacccagta agcctggtaa tgatttggat ccatcgcaat cactgcgcga
      481 agctgctcga tggcctcatc gtagcgacgc gcattctcca gtaagaatcc tctttgtaca
      541 ctgatcgcaa gcgaaaacgg atccagctcg cgcgcacgat tcgacgccgc gatcgactcg
      601 tcaacacgcc cgcgcgacat caaatagctc gcgtaaaagt tgtgcgcgtt tgcgtaattc
      661 ggattgagct cgagcgcgtg tttgaaacgc tgctcagccg cggtccagtt ccagttgtag
      721 tgatttatgt aacccagcgc cgtgtaagca tcggcgagac tgccgtcgag ctcgagcgcc
      781 ttcgacgccg cctcctctgc gcgtcgtctg ccctcgaccg gcggcaacgc actaatctga
      841 accacgctga gtccgttgta gcaatcagca aggccaacat acgcgggcgc atagcccgga
      901 tcttccttga ttgcattctg aaaaagcccg atcgccttgt tgagattttc ttcggtgcgt
      961 ttattccagt agagataacg cccctgtaaa tagctgtcgt aggccttcgg ttgcaccgcg
     1021 cggcgcgcag tgaaacgcgc ctgctcgccg ggagtcaact ggatctgcac ctggcgcgca
     1081 acgtcctgcg cgatctcact ctggagatcg agaacgttgc gcacgtcacg cgtgtacgtt
     1141 tggacccaca ggtgccggtc cgtggccgct tgtattagct gcgcccgaac gaccacgcgc
     1201 tcgcccgatc gttgcacggt tccttcgacg acggcgtcga cgttgatctc accagcgatc
     1261 tgcggcagcg atttctttgt tcctttgtag tgcatcgacg aagttcgcga aataacatgc
     1321 agcccaccga tctgtgcgag gtctccgata agtgcgtctg tcattccgtc agcgaaatat
     1381 tcttccgcaa cgtcaccgga caagttctcg agcggcagca ccatgatgga cgagatcgca
     1441 ggctgtggtt tttctcgcct gtaccacgcg aaagcgatcg ccaccagaac taacgggaca
     1501 aggatcagca gcgcataacg aaacaccggt ctcggccgcg tcgcccgctc ttccgctgcg
     1561 tcgtggcgtc gctgcgcggg ctcttttcgg acttccgcga caaaccggta cccgacgccc
     1621 ggaatcgtcg cgatgtactg gctctcctgc cggctatccc caagcgactt tctcaacgcc
     1681 gtaatattct ggttgagatt attttcctcg acgatggttc ccgcccacac gcgctccagc
     1741 aactcatcct tctcgaccgt ccgcccatgc tcttccacga gcacgagcag cagctcaaac
     1801 gccttcggag tcagccgcag cgtctcttca tccttcgaaa gtcgacgctt caacgggtcg
     1861 agccgaaacg gcccaaactg gtagaaatgc agattttcct cagacattca ggctttcaga
     1921 aactctttca gaaaaaatca gcgggtctct aagtacattt tcgctcgccc tcgcgtagtt
     1981 tccgcaccgt tgagcgaccg actttcaagc agtttggaag gcctgctcat tctaacgctg
     2041 acggaaggaa acgaaaatga aactcaaagg tctaaaaaat atttttttga tattcgcagt
     2101 cttaatctgc acccagacaa gcgcctgggc cgctcaggaa gatgtcatcc tgcaatggaa
     2161 ccgtgtgttg atggagacgg ttcgcactcc gggacagcaa ccagcgacca tcatgcccgt
     2221 acgcagctac gccatcatgc acgcggcaat gtttgacgcc gtcaactcca tcgagagaag
     2281 tcatacgtct tactttgttg acgtacccgg atcgcctaac gcttcgattg aagccgccgc
     2341 ggcgcaggcc gcgcacgacg tattggcggg tttgtatccg acgcgcgtgg cgatctttaa
     2401 tgcggagctg gctatctcgc tgcagggcat cgaagagaat cgagcccagc aaggaatcag
     2461 agtgggccag atcgttgccg aaactttgct cgcggaacgc gcaaacgatg gttggaatgt
     2521 cacgccgcct gcctacagct taccggcaac gccgggaaac tggcagccgg cacccggtgc
     2581 ggcgacgttt acacattacc cctcggtcat acctttcggt atcacgtcgg gcctgcagtt
     2641 caaaccgaat gcaccaccgg cgctcacgag cgccgaatac gctaacgacc taaacgaagt
     2701 taaggagctc ggatcggtta gcagtttgac gcgcactgcg gatcaaacca aggtcgcgca
     2761 gttgtgggcc ggggtgaata cgccgactaa ctttctcttc gtgtggaaca atgtcgcgcg
     2821 cactgtttct gcagcacgtg gtatctccac agtcgagaaa gcacgattgt ttgcgttgac
     2881 gaacatcgcg cttcacgacg cgttacaaac cacgttcgcc agcaagtttc actatggctt
     2941 gtggcgaccg gtgacggcga ttcgacgtgc ggatgaagat ggcaatgcga gtacggcagc
     3001 tgacttgacg tggacttcgc tgatcgcgag tccgccgtat ccgagttatg cgggaaacaa
     3061 cgcggcgatc ggcacttcgc aagcgacgat actcgcgttg ttcttcggac gtgatgacat
     3121 cgcgttccag cacacgtggg aaggtgcagg cggcgcgacg cgttcgtacg cggggttcag
     3181 tgcaatggcc gacgaagagg cccgcgcacg tgtctacggt ggaattcact tcaccttcga
     3241 caacgtcgcc ggacaatcag tcggccgcaa cgtagcgaac tacatcttcg ccaacgtgat
     3301 gcgcccccgg cagtgtgttc gttaagcccg cgaagcgggc gacagcataa agcctggggc
     3361 gcaagcccca ggatcgaagt cgaaaataca atttgagccc gcagagtggg cgacagccca
     3421 aacatcgacg cgctgtcgcc cgctccgcgg gctcgttcgc attttgctaa tcgatcctgg
     3481 ggctcgcgcc ccaggcttta tgctgtcgcc cgctttcgcg ggctcggcgc tctgcttcac
     3541 ggctctatgg ataatggagg gtcacgttgt tgctgaggcc cagattccgc cgcgctttct
     3601 gctcaaattt gcgcgcgggg ttggtcatgt agaggccgca acatcgattg cagatcggca
     3661 gacggttcgt gaggatgtac ttgcgaaatt tgaccgccat ctcggaattg aacgactcca
     3721 taaatccatc gcgaaaaaca tttccggcaa taacgtcagg atggcccgg
//
LOCUS       DASSFQ010000046         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1508612, whole genome shotgun sequence.
ACCESSION   DASSFQ010000046 DASSFQ010000000
VERSION     DASSFQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508612"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFH96_01500"
     CDS             complement(<1..203)
                     /locus_tag="VFH96_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HET6972665.1"
                     /translation="MLSVALVEPEIPPNTGNIARLCAATNVPLHIVGVTGFRMDDRAV
                     RRAGLDYWPEVNLTRHKDLAALY"
     gene            complement(814..1542)
                     /locus_tag="VFH96_01505"
     CDS             complement(814..1542)
                     /locus_tag="VFH96_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972666.1"
                     /translation="MTNLKTIVDHKICGSSSIDSASSSVAVSVSRRRSEGGYTLVALL
                     AMMTVLALFAMAAAPSIQQQAQREREQEAIFRGEQVADAIRDYYRYRSGTIRAVGAQA
                     LPTSMDQLLQGIPIPGGSKTRQILRPSAARDPLTLEGEWRYILPRSEALIDFQQSIMF
                     YTDNVIPPTRDPQMAQLQQFAVPQILNTVNLGTQSATRDQSSVDDDATGPFVGVASRS
                     KRDSVLTYYGIERHEQWIFTPLFR"
     gene            complement(1529..2452)
                     /locus_tag="VFH96_01510"
     CDS             complement(1529..2452)
                     /locus_tag="VFH96_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972667.1"
                     /translation="MALVDLSKPGEKKKLIFAGVLGFGAILILYWALIGFDSRPTPQT
                     RPTASASPQRSTTTTTTQRPATAPPVAQQVVDVARFAPIDYEPSSYNAPEAKRNIFAY
                     YEPVKPAPVVVVTPTPTPTPPPPILLASVSPSNVYARTADFKLEAAGDKFTPEMRIFV
                     DGRELPTAYKSPQQLSTTMPASIITAPGARQIVVRTPDNRLFSNQLTVNVAAPPTPNY
                     TYIGIISPQNRVTDTAIVQDRSNRNVVSVLRGDVIGGRFRVTSISEKELVVVDTSLKI
                     KHILPMTEGDKTAGGPLSRPTPRVDSEDDEP"
     gene            complement(2458..3213)
                     /locus_tag="VFH96_01515"
     CDS             complement(2458..3213)
                     /locus_tag="VFH96_01515"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HET6972668.1"
                     /translation="MSAEIQTEPRSTGYKLRVSEFLESRRGKMFGVAEVVALAGSCLV
                     FALVLMSYIYFLIPARSRLASLKVDLKQTQTNLQTLDTLINKDQTTKQTVDRAAASLD
                     KFETDHLLRVTQGRMDLYEELNQLIVKNNVRNTSGPTYTTLDAAGTKSNSGKTVITKW
                     QSFYPGIAVMVTVEGEYQNVRRFIRDVERSRQFVVINEVELQRASQNNAPASAEEGGA
                     GSGTRGSLVTLQLNMATYFQRPGAADNVPASQE"
     gene            complement(3210..>3625)
                     /locus_tag="VFH96_01520"
     CDS             complement(3210..>3625)
                     /locus_tag="VFH96_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972669.1"
                     /translation="QAFTPDQQQALPAAHELVDRKSFSWSRLLADLESVLPANVKVSR
                     IAVRDVTRAGGQTVAQLDLAVFATDPSTISQMMTDWQQGGVFLAEIRVQNLQKGRGEK
                     GTEYELAVTYRAPSAYSSENVAAVDATRKESEVRR"
BASE COUNT          757 a          989 c         1008 g          871 t
ORIGIN      
        1 tcgtagagcg cagcgagatc tttgtggcgc gtgagattta cttccggcca gtagtcgaga
       61 cctgcgcgtc gcactgcgcg atcgtccatg cgaaagcctg tgacaccgac gatatgaagc
      121 ggcacgttgg tggcagcgca caaccgcgcg atgttgccgg tgttgggtgg aatctcaggt
      181 tcgacgagtg cgacggaaag cataggtcgt attagtccta tcagacctat atcatgaaat
      241 acaaaaaaga aacgacgagc ttcgcttgaa ggtctcgtcg cttcttttgt aaggcctcgt
      301 cggcctcaga tgtttgtgtg cgccggtgga aggtacgtac tgtaacctgc tggttcgggt
      361 tatctcttct tcgcggcctt cttgcctccg cccttctttg cggccttctt ggttccgccc
      421 ttcttacctc cggctttctt tccacctttt ttagttgcca tagcttctat gtgctccttc
      481 tttcgttatt gggttttact gtttcttcat gaccacaaga tactgtgctt acttcgttct
      541 tgtgggctag agtaaacggc gcacaacatc ttgctatata aacgcaatta tcacggatgt
      601 caataactta ttactccatt acagaaaaaa tttttgcagc agcaagcggc gagatccgcg
      661 tgcgcgagag tctcatcgac ccgcagcggc acacttggac catgcagatc gcgtcaacac
      721 ttcgcgcgat cggatctcat caccagccta tgtgtgcaac tgcgagtaaa gaattaattt
      781 gaagtggagt gaaagcagat ggtccaacta cgactagcga aacagcggcg tgaagatcca
      841 ctgctcgtgt cgctcgatgc cgtagtaggt caacactgag tcacgcttgc tgcgactcgc
      901 aaccccgacg aatggaccag tcgcatcatc gtcaactgac gactggtcgc gcgttgcgga
      961 ctgcgtgccg agattgaccg tgttaagaat ttgcggaact gcaaactgtt gtaattgcgc
     1021 catttgcgga tcgcgtgtgg gcggaatgac gttgtccgtg tagaacatga tcgactgctg
     1081 aaaatcgatc aaggcttcac tgcgaggcag gatataacgc cactcgcctt cgagggttaa
     1141 gggatcgcgc gccgccgaag gacgaagtat ttgtctcgtc tttgagccac cgggaatcgg
     1201 tattccctgt agcagttggt ccatcgatgt cggcaatgcc tgggcgccga cagcgcgaat
     1261 agttccggag cgataacgat aataatcacg gatcgcatcg gccacttgct caccacgaaa
     1321 gatcgcttcc tgctcgcgtt cgcgttgcgc ctgttgctgt atgctgggcg ctgctgccat
     1381 cgcaaagagg gcgagcaccg tcatcatcgc gagcagcgcg acgagcgtgt aaccaccctc
     1441 agatcgccgt cgcgacaccg aaacagcgac agacgaggac gctgagtcga tcgagctcga
     1501 cccgcatatc ttgtggtcga cgatcgtctt aaggttcgtc atcttcagaa tcaactcgtg
     1561 gtgttggacg cgacaaagga ccaccagcag tcttgtcgcc ttcagtcatt ggcagtatgt
     1621 gtttgatctt caaactcgtg tctactacca cgagctcttt ttccgagatg ctggtcacac
     1681 gaaacctgcc accgataacg tcgccgcgca gtaccgacac gacgttgcga ttgctgcgat
     1741 cctgaactat cgccgtatcg gtaacgcgat tctgcggact gatgatgccg atataggtgt
     1801 agttgggcgt tggcggcgcg gccacgttga ccgtcagttg attcgagaac aaacggttgt
     1861 cgggagtgcg aacgacgatc tggcgtgcgc cgggagcagt gatgatcgac gccggcatcg
     1921 tcgtcgaaag ttgttgcggg ctcttgtaag cggtgggcag ctcacgacca tcaacaaaga
     1981 tgcgcatctc gggtgtgaac ttgtcaccgg cggcttcgag tttgaaatcc gcagtccgcg
     2041 cgtaaacatt cgagggcgaa acagacgcga gcagaatagg cggcggcgga gtcggagtcg
     2101 gagttggcgt tactacaact acaggcgccg gctttaccgg ctcgtaataa gcaaaaatat
     2161 ttcgcttcgc ctcaggcgca ttgtatgacg atggctcgta atcgatcgga gcaaacctgg
     2221 caacgtccac gacttgctgt gcaaccggtg gggcagtagc cggccgctgc gtggtcgtcg
     2281 ttgtggttga tcgctgcggc gacgcgctcg cggttggcct ggtctgcgga gtcggacggc
     2341 tatcaaaacc aatcaatgcc cagtacagaa taaggatcgc tccgaagccg aggactccgg
     2401 caaagattag tttcttcttt tcacctggtt tgcttagatc gaccagtgcc atttcgttta
     2461 ttcctgactt gccggcacat tatccgcagc gcctggacgc tgaaagtaag tcgccatgtt
     2521 tagctgtaac gtcacgagcg aaccgcgcgt gcccgaaccc gcgccgcctt cttctgctga
     2581 cgccggtgcg ttgttctgac tggcacgctg aagctcaact tcgttgatga ctacaaactg
     2641 cctgcttcgc tcgacgtcgc gtatgaagcg acgcacgttt tgatattcgc cctcgaccgt
     2701 caccatcacc gcgatgcccg ggtaaaaact ctgccacttc gtgatcacag tcttgccgga
     2761 attcgacttc gtgcctgcgg catcgagcgt tgtgtaagtc gggcccgacg tattgcgcac
     2821 gttgttcttt acgattagct gattcaattc ttcgtacaag tccatgcgac cctgcgtcac
     2881 tcgcagcaga tggtcagtct cgaacttatc caggctcgcc gccgcacggt cgacagtttg
     2941 tttcgtggtt tggtccttgt tgataagagt gtcgagcgtt tgcaggttcg tttgcgtctg
     3001 tttcagatcg accttcaacg aagccagacg agaacgagcg gggatcagaa agtagatgta
     3061 actcatgagg acgagcgcga acacgaggca actgccggcg agtgcgacaa cttcagccac
     3121 tccgaacatc ttgccgcgac gtgactcgag aaactcgctc acgcgcaatt tataaccagt
     3181 cgatcgaggt tcggtttgta tttctgcgct catctccgca cctcgctttc tttcctggtt
     3241 gcgtcgactg cagcgacgtt ctccgatgaa taagcggagg gtgcacggta agtgacggcg
     3301 agctcgtact cagttccctt ttcgccacga cccttttgca ggttctgcac gcgaatctcg
     3361 gccaggaaaa cgcctccctg ctgccaatca gtcatcatct ggctgatcgt ggatggatct
     3421 gtcgcgaata ctgcgaggtc gagttgggcg acagtctgac cgcccgctcg cgtcacatcg
     3481 cgcactgcga tgcgcgagac tttaacgttc gccggcagca ctgactccag atcagccagc
     3541 aatcgagacc atgaaaatga tttgcggtcg accagctcgt gtgcggctgg aagtgcctgc
     3601 tgctggtcgg gcgtgaacgc ttgtt
//
LOCUS       DASSFQ010000047        14393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1660236, whole genome shotgun sequence.
ACCESSION   DASSFQ010000047 DASSFQ010000000
VERSION     DASSFQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660236"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFH96_01525"
     CDS             complement(<1..908)
                     /locus_tag="VFH96_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972670.1"
                     /translation="MVKFLVAFILFTSSVVAQQIGNPTTQQGVLPPRGTFAIRNARIV
                     TVSGPDIENGTVVIRDGKIEAVGTNVSVPAGAQTIDGRGLSVYPGMIDAGTNMGLVEV
                     PQGANGTVDLSEVGDMNPNAKAIIAVNPHSAHIGVTRVEGITNTLTAPTGGLISGQAA
                     LINLLGTAPKEMAVVPQAALVINYPRIGFGGGGFGGGFQQPANLADTLTANERQVEQI
                     RKMLRDAEAYGRAQDAYAKDKSLPRPDRNVVLEPLVPYVRGEQPVIFRADREAEIRGA
                     IRFADEMKLKPIILGGNDAWKVASLL"
     gene            complement(975..2360)
                     /locus_tag="VFH96_01530"
     CDS             complement(975..2360)
                     /locus_tag="VFH96_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6972671.1"
                     /translation="MKRILVLLAFIVVSVSAVQAQTETLIRNATVLTVTQGTLQNTDV
                     LLRNGKIAAVGKNLKASANARVIDATGKFVMPGIIDCHSHSMLDTINEGTLAVTSMVR
                     TQDVLNTTDVDLYRELAGGVTTLNLLHGSANPIGGLNTVVKIKFGRPASEFLFPGAMP
                     GIKFALGENVKRSSTPNLPGVPRRYPNTRMGVEETIRDAFTRARDYKRTWDEYREAVK
                     KDKNLLPPRRDLQLEPLVEILEGKRYVHAHCYIASEILMLINIADEFGFKIKTFQHVL
                     EGYKVAKEIAAHGAGASIFADSWGYKIEAYDAIPYNAAILTRAGVIVSMNSDSDERAR
                     RLNIEAAKAMHWGDLTEEQALKLVTLNPAIQLGIQERVGSIEVGKDADVAIWNGHPFS
                     VYSRVDTTFIDGDVYFDREKDLARRPELAKERATLEQAEPNKPPARGQSPLTPRRRPS
                     GHEDDAEVEKP"
     gene            complement(2393..4075)
                     /locus_tag="VFH96_01535"
     CDS             complement(2393..4075)
                     /locus_tag="VFH96_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6972672.1"
                     /translation="MPKVRSRLLVASLLLLLTVNPLHAQRSAIDTYAITNARIVTVSG
                     PVIERGTVVIRNGLIAAAGANVSAPSDARVIDGAGLTVYPGLIDSYTNLALPEPAPGP
                     SPGGGGGGGGFFLLQQLQQQRPPSGPNSTQPAGLQPEVMIEDVIRPGGNEIESWRSAG
                     VTTALTTPRSGIWMGQSALINLSGDTPQQMIVRSPVAMHVGFTPLRTGAYPGSLMGVF
                     STLRQMMLDAQRYRDAMQIYERNPRGTRRPDTDRSLAALIPILDGRMPVVMIANSERE
                     ISRALDLASEFKLKLIVAGGQEADKVADRLAKQNVPVLLSLNLPRRTTAALPEADPEP
                     MRVLRERVEAQQTAGKLAKAGVRFGFQSGSMANSSDLLSNANKTIENGLQPLDALRAF
                     TIWPAEILGVKDQLGSIEVGKIANLTVTRGDLFDRNSRIAHVFIDGRPVDLRAAAPGG
                     GPGQGRNSLAGQWTVVVNTGQGDRTISLTLQQEGERLTGSISGPLGAGEISNGSASNT
                     GEVRFTVTVNVEGQTKEATFTGNLANNQIRGNVAIVGSAPGTFTATRSGQPN"
     gene            complement(4217..4738)
                     /locus_tag="VFH96_01540"
     CDS             complement(4217..4738)
                     /locus_tag="VFH96_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6972673.1"
                     /translation="MRRRITGFIALAALAVLLPACDNTANQNTTANRNANAVATATAT
                     PTPVANTNSNRAPTRAEYEANKERYNREAKESGRKVGTGANDTWLWIKTRFDLAAADD
                     LRDSTINVDVDNDIVTLSGTVASAAQKTRAEAVAKSVAGVKSVRNQLKVQAGNANANA
                     NASPARGGTNRNR"
     gene            complement(4873..5157)
                     /locus_tag="VFH96_01545"
     CDS             complement(4873..5157)
                     /locus_tag="VFH96_01545"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET6972674.1"
                     /translation="MADTIKCTHCGEKHPALPFAPFPNELGQRIAAEICQPCWSGWLQ
                     KQTQIINHYGLDLTNPDAQTFLFDNIKGYLFGDVPTVAEIDTTKEGTVKW"
     gene            complement(5240..5707)
                     /locus_tag="VFH96_01550"
     CDS             complement(5240..5707)
                     /locus_tag="VFH96_01550"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET6972675.1"
                     /translation="MSNDAAVNTTAPDFTLKDGNGGEWRLSDHRGKVVVLLFYPGDET
                     PVCTRQMCSLRDRWDDYLATGAEVVGISSDSIESHKKFAAHHNLPLRLLSDSDGAVST
                     LYGARSLIPGRVARSVFVIDGSGIIRHRDVRPIGLFKPKDDDNIAAIKAADVR"
     gene            complement(5704..6849)
                     /locus_tag="VFH96_01555"
     CDS             complement(5704..6849)
                     /locus_tag="VFH96_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6972676.1"
                     /translation="MIANRHRDEFPTLASGIHLLSHSLGPVPRAAHESMLSYIDAWEH
                     HTSEDAWATSWWTLSRQVGDRIATILAADAGSVQIQPNASIAMATVASCFDFNKGRNK
                     VVTTALDFPSMEYFWDAQRQIGARVEVVPSSDNISVPLERILDAIDNETALVALAHTS
                     FCSSYRIAARAIVEHAHSKGALVLLDVYQSAGVVQLNAAGWNVDFLIGGTIKWLCGGP
                     SCGYLYVRPDLQRDLRPRLTGWVAHDSPFDFAHAPMRYARSVRRFAQGTPSIPALYSA
                     IPGLEIIEAVGVSAIAAESERRTQLMIDFASERGWTINTPREKSQRGGSVMIGVNEAP
                     AMVERLAERRVFVDSRPSAGLRMSPHFFNTDEEVEEALNILAELIES"
     gene            complement(6898..7617)
                     /locus_tag="VFH96_01560"
     CDS             complement(6898..7617)
                     /locus_tag="VFH96_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6972677.1"
                     /translation="MQIEGKRVLVTGASRGLGRALVTAFLDAGASEVIAGARKPEDRE
                     ALKSARVTPVQLDVTIADDVEELVHLGAIDILVNNAGIAGFGNPLTMDFAAVQEEIAV
                     NYLGVLRVTRAVAPGMIAQRDGVIVNVATAFAKVNLPIVGTYCATKAALLSLGQAMRA
                     YLSEHNVRVITVMPTTIDTDMSKGANVPKMTKEFVAGEILAAIREERHDPPIGDEAKG
                     VFENLSKDALGLEKVLQDYRM"
     gene            complement(7607..8998)
                     /locus_tag="VFH96_01565"
     CDS             complement(7607..8998)
                     /locus_tag="VFH96_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6972678.1"
                     /translation="MKLRRDLGTVESYATVIGILIGAGIFQVTSQAWALTGPSVILGY
                     LVLTPAILATSIPYAAYLSTPLGREPGGEYLHISRTVGGFRLAYIGCWLKIISYIGAG
                     AYLANAMAGYIIALSGNRIDAGTYRLPLALAALVVFYLIHVIGIRWFGRFQVLMSALK
                     CLALLVLIVPGLFVLHSENYVPFFTHGPSGFGASLLPLFFAYAGFESIAQTAGEVKDS
                     TRRLPKILLKGITITALIYVFTSIVSFGALPGGRLQASSAPMAEVASIYLPAGAAMFV
                     TVGAIAAIMTALNRTIFVPSRLAIMFVEDGLAPRWLGFINKATATPIRALTLTLICCA
                     ILLVSGQLALALNVAVFALVVLYFIHSLVFLLLPRLNPSLSSEIKISLSHRLQQAAAI
                     VSLLSMGLMIVIQLRQDAAVLSTQTLSQRISGQSLTSVELIVVWGVIGAALYQLARMQ
                     RGGEAAKLGAHAD"
     gene            complement(9025..10212)
                     /locus_tag="VFH96_01570"
     CDS             complement(9025..10212)
                     /locus_tag="VFH96_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6972679.1"
                     /translation="MIKIKSFDGGEFDGYLALPASGYGPGIVVLQEIFGINQYLRDVA
                     DWYAAHGFVALVPDLFWRIQPNIELTDKGDDWKRAVGLYQQIDEAKAVEDSAATMELL
                     RKHPACSGRVGAVGFCMGGKLAYLLSVRYKPDCAVGYYGVSIEKSLDEAENLDSPLLL
                     HIAGLDKFCPPEAQAQIHAALDAKATIHDYPDADHAFARTGGEHYDLAAAELANLRSL
                     KFFVNHLAGIGLASAQQILSQRWDDHVKYEFATRNTDDTLETMVVDAYVNHVPVMTGG
                     VGHDELREFYSTRFIPQMPPDTAMTPVSRTIGVDRVVDEMVFEFTHSIKMDWMLPGLE
                     PTGKHVKVALVVIVHFRDGKLAHEHIYWDQASVLAQLGLIDSTKLPVVGVESAEKVLN
                     PRN"
     gene            complement(10261..14091)
                     /locus_tag="VFH96_01575"
     CDS             complement(10261..14091)
                     /locus_tag="VFH96_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6972680.1"
                     /translation="MEQRVLLPEQAAAAYEIDKHISVTAGPGSGKTTVLVERYVHILR
                     EHSLSIDQIVAITFTNRAANEMHERLRSELNQMLRIANEEERRRWLNYKRTLDGAVIT
                     TIHGFCARLLREFPIEARVDPQFLLLDEHRAAMMLELIVEEVLSEFISSGHVEISRLT
                     LGVGRSRLAAALAQLYRQARGQGLALEDLALATARVHATEEDHVAALEELERTMSEFL
                     AVRRTTKAAMTNREEVLAAWTEAQKLLKSIPSHETLADFCRLVENFRAHRPQARASVA
                     DHVKALDTLVWEKGLQGRVPQVCLDLFARQYALEMVNLLLRVDQRLNEEKQKLSALDF
                     DDLELRTLELLQRPEVVARTSERYRFFLVDEFQDTNAVQRVLLERLALAKDRRPANLF
                     IVGDRKQSIYGFRGADVDVFREMTSILLAARGEEKPLQLNFRSQPPLIAFFNYLFARL
                     FQVPDDIPASEYKNLDQLGYVKHEKSEPKRELRDSDSLKGPLVELLVTTKPSLEDDPR
                     AEQDSRVLDAQQLARRILALTQGPDKLKYGDIALLFRAMTQVQIYESVFRRANIPYQT
                     VLGRGFYEREEITDLIQLLRFLDNKTDEIALAAVLRSPLCGLSDNALLGLRCAPLLNE
                     VETVHGFSATRPLYTALRHHEEIAFINPEEHELLDRAAKLIGELVDRRHHYPIQDLLR
                     YAVEESEYLTVVAANYDGAQRLANDERLFTLAARFESSGTHLIRDFVRYVEEFEAIGS
                     RESEGQIDQAADAVRLMTIHQAKGLEFPVVIIPDLQRLSRVATDNWVLLDRHQGLTLK
                     VPDGRGNLVAGCTFSNFEKRHAWREQFESMRLLYVAATRAEDRLIFSGVTEDVASLAS
                     KSDSWLKWIWQSLELHDRHASDLIDLDDDVQLQLTMNLADEPPDEFEPQPEQPPQTSM
                     HSADSLSEAFPLIKPFGQQTGNAIHRFSVTQLINYQRCPRQYYFDRVLQLPAADQMAV
                     WNNAEAPEPPSNLTATLKGSVIHRFCERYTPDQSVEGCLRTSFEEVLRLRQAQLSDRF
                     VEIDHERAIRAMMPLAKNYLASDVYKRVEAVPKIAEHYSPRPAGAEGLWSELSFRLRR
                     RLGVLNGIIDKLLITDSEIEIIDFKTNRVVYDKSETPREPPAPRIRAPKGGQFAFDFE
                     DEGPPQPVNRHPSLEEALKTAAEDYQLQMQAYALAVRELLPSLAGANVRVTLHFLDPN
                     VEVHLADELLEPARCEQAVDEGMQRTIFSSDPANFPVKTAPHCRMCNFLSVCEAGKKW
                     IAENWSTDSADYTE"
     gene            complement(14151..>14393)
                     /locus_tag="VFH96_01580"
     CDS             complement(14151..>14393)
                     /locus_tag="VFH96_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET6972681.1"
                     /translation="KGLYRASQLDYLNLRAKNSVFTDEDWTRIREQVTARIWEFLDGI
                     RSGRFIVDPSERLKTCRFCDYSAVCRYDRDRIERKK"
BASE COUNT         3063 a         4248 c         4014 g         3068 t
ORIGIN      
        1 ttcaacaggc tcgcgacttt ccatgcatcg ttcccgccga gaatgatcgg cttgagcttc
       61 atctcgtcgg cgaaacgaat cgcgccgcga atctccgctt cacgatcggc gcggaagatc
      121 actggctgct cgccacggac gtatggcacg agcggttcga gcacaacgtt tcgatccggg
      181 cgtggtaacg acttgtcttt cgcgtacgca tcctgcgcac gaccgtaagc ctcggcgtcg
      241 cgcaacatct ttcgaatctg ttcgacttgt cgctcgtttg cggtcagcgt atccgcgaga
      301 tttgcgggtt gttggaagcc gccgccgaaa ccaccgccgc cgaagccgat gcgcggatag
      361 ttgatcacga gcgcggcctg cggaacgacg gccatctctt ttggcgctgt gccgagcagg
      421 ttgatcagcg ccgcctggcc ggagattaga ccaccagtcg gagctgtgag cgtattagtt
      481 atgccttcca cgcgcgtgac gccgatgtgc gcactgtgcg ggttaacggc gatgatggct
      541 ttcgcgttcg ggttcatatc accgacttca gaaagatcca cagtgccgtt cgcgccttgt
      601 gggacttcga cgagacccat gtttgtgccg gcgtcgatca tgccgggata gactgacagg
      661 ccacggccgt cgatggtctg cgcgcctgcg ggaacggata cattcgtgcc gacggcttcg
      721 atcttgccgt cgcggatcac cacagtgccg ttctcgatgt ccggtccgct aactgtgacg
      781 atgcgcgcgt tacgaatcgc gaacgtgcca cgcggcggaa gcacgccctg ctgcgtagtt
      841 gggtttccga tctgctgcgc gacgactgaa ctggtgaaaa ggataaatgc aactaggaac
      901 ttgaccatgc tatctccatt tgggccacgg ataacacgga tcgaaatccg gatttttatc
      961 cgtgttatct gtggttacgg tttttcgact tccgcgtcat cttcgtgacc gctcggacgg
     1021 cgacgaggcg ttagtggtga ctgaccgcgt gccggtggct tgtttggctc agcctgctcg
     1081 agtgtcgcgc gttcttttgc gagctcgggg cggcgtgcga ggtccttttc acgatcgaag
     1141 taaacatcgc cgtcgatgaa cgtcgtatcg acacgcgaat agacactgaa cggatgccca
     1201 ttccagatcg cgacatccgc atccttgccg acttcgatcg agccgacgcg ctcctgaatg
     1261 ccgagttgaa tagcgggatt gagcgtcacg agtttcagcg cctgctcttc agtcaggtcg
     1321 ccccaatgca tcgccttcgc agcttcgatg ttcaaacgtc gcgcgcgttc gtcagagtcg
     1381 gagttcatcg acacgatcac gcccgcacgc gtcaggattg cggcgttgta aggaatcgca
     1441 tcgtaagcct caatcttgta gccccacgaa tcggcgaaga tcgaagcgcc cgcgccgtgg
     1501 gcggcgatct ctttcgcaac tttgtaaccc tcgagcacgt gctggaaagt cttgatcttg
     1561 aaaccaaact cgtccgcgat attgatcagc atcaggatct cactcgcgat gtagcagtgc
     1621 gcgtgaacgt agcgcttgcc ttcgaggatc tcgacgagcg gctcgagttg caaatcgcgg
     1681 cgcggcggca gcaggttctt gtctttcttc acggcctcgc gatactcgtc ccaagtgcgc
     1741 ttgtaatcac gcgcgcgcgt gaacgcatcg cgaatcgttt cctcaacgcc catgcgcgtg
     1801 tttggatagc ggcgcggaac gccgggcagg tttggcgtgc tcgatcgctt cacgttctcg
     1861 ccgagcgcaa acttgattcc cggcatcgca cccggaaaca aaaactcact cgcggggcgg
     1921 ccgaacttga tcttgacgac agtgttcaat ccaccgatcg gatttgcaga accgtgcagt
     1981 agattcagtg tcgtgacgcc gccggccagc tcgcgataga gatcgacgtc ggtcgtgttc
     2041 agcacgtcct gtgttcgcac catcgaggtg acggcgagcg tgccttcgtt gatcgtgtcg
     2101 agcatcgagt gagagtggca gtcgataatg ccgggcatga cgaatttgcc ggtcgcgtcg
     2161 atcacacgcg cgttcgcaga tgccttgagg ttcttaccga cggcagcgat cttgccgtta
     2221 cgcagtagca catctgtatt ctgaagcgtg ccttgcgtga cggtgagcac ggtggcgttg
     2281 cgaatcaacg tctcagtttg tgcctgtact gcactaacac tgacaacgat aaaggctaga
     2341 agaactaata ttcgtttcat tgttttttgt gccctttgtg cctttttgcg gctcaattag
     2401 gttgacctga acgtgttgca gtgaatgttc cgggcgcgga gccgacgatc gcgacgttcc
     2461 cgcgaatctg gttgttcgcg agattgccgg tgaacgtcgc ttccttcgtt tgcccttcga
     2521 cattcaccgt caccgtgaac cgcacctcac cagtgttcga cgccgatcca ttcgagatct
     2581 ctcccgctcc cagcgggccc gagatcgatc cggtgaggcg ctctccttcc tgttgcaacg
     2641 tcaaactgat cgtgcggtcg ccctggcccg tgttaacgac gacggtccac tggccagcta
     2701 aactgtttct tccctgaccc ggaccaccgc caggcgccgc cgccctgaga tccactggac
     2761 gcccgtcaat aaacacatgc gcgatgcgac tatttcgatc gaacagatcg ccacgcgtga
     2821 ccgtcaggtt tgcaatcttt ccgacctcga tcgatccgag ctggtccttg acaccgagaa
     2881 tctcagccgg ccaaatcgta aacgcgcgca aagcatcgag cggctggagt ccgttctcga
     2941 tggtcttgtt tgcattactc agcagatcgg aactgtttgc catcgatccc gactggaaac
     3001 caaaccgtac gccggccttc gccagcttgc ccgccgtttg ttgagcttcg acgcgctcgc
     3061 gcagcacacg catcggctca ggatcagctt cgggcaacgc ggccgtagtg cgtcgcggca
     3121 gattgagcga caacaacacg ggcacgttct gtttcgccag tcgatccgcg actttatccg
     3181 cctcctggcc gcccgcgacg atcagtttca acttaaactc actcgcgaga tcaagcgccc
     3241 gactgatctc gcgctcgctg ttcgcaatca tcaccaccgg catgcgtcca tcaaggatcg
     3301 gaatcagcgc cgcgagcgat cgatccgtgt cgggccggcg cgtgccacgc gggttacgct
     3361 cgtaaatctg catcgcatca cgataccgct gcgcgtcgag catcatctga cgcagcgttg
     3421 aaaacacgcc catcagcgat ccgggatacg ctccggtccg cagcggcgtg aaacccacgt
     3481 gcattgcgac aggcgaacgc acgatcatct gctgtggtgt gtcgcccgag agattgatca
     3541 gtgctgactg gcccatccag atgcctgagc gcggtgtagt caacgcggtc gtcacaccgg
     3601 cgctgcgcca cgactcgatc tcatttcccc ccggacggat cacgtcttca atcattactt
     3661 ccggttgcag accggccggc tgcgtcgagt ttggaccact cggcggtcgc tgctgctgta
     3721 gttgttgcag gagaaagaac ccgccgccac caccgccacc tcccggcgaa ggaccaggcg
     3781 caggctcggg caacgcgaga ttcgtgtacg agtcgatcaa acccggatag accgtcaagc
     3841 cagcaccgtc gatcacgcga gcgtccgaag gcgcgctgac gtttgcgccc gcggcggcaa
     3901 tcaacccatt gcgaatcacg actgtgccgc gctcgatcac tggtccggag acagtgacga
     3961 ttcgcgcgtt ggtgatcgca taagtatcga tagcgctgcg ttgagcgtgg agcgggttaa
     4021 cggtaagaag caacagcagg gaagcgacaa gaaggcgcga acggactttt ggcataggta
     4081 accaaatccc catcgttttg atttttgcga aaaacagagg aaagaaagtg aagattagca
     4141 tacggattaa acggatccaa agcacggatt tacgctgatc tgtgtaaatc cgtgtttgaa
     4201 atccgtctaa tccgcgttat ctattccggt tcgtgccgcc cctggcggga ctggcgttcg
     4261 cgttggcgtt cgcgtttccg gcctgcacct ttaactggtt cctaacgctc ttaacgcctg
     4321 caacactctt cgctactgct tcggcccgcg tcttctgggc tgcgctcgct accgtgccgc
     4381 taagcgtcac gatatcgtta tcgacgtcaa cattgatcgt cgaatcacga agatcgtcgg
     4441 cggcagcgag atcgaagcgc gttttaatcc aaagccaggt gtcgtttgcg ccagtaccga
     4501 ccttgcggcc cgactccttc gcttcgcggt tataacgttc cttgtttgct tcatactccg
     4561 cgcgcgtcgg cgctcggttg ctgtttgtat tggcaacagg cgttggcgtc gctgtcgcag
     4621 tggcaaccgc attcgcgttt cgattggcgg tcgtgttctg gtttgcagtg ttatcgcaag
     4681 cgggcagtag tactgcaagc gcggctaaag ctatgaatcc tgtgattctg cgtctcatca
     4741 aatccctcct tacaagtgaa aacaagtgaa tacacaaatc caagttcgga aaacatacgt
     4801 tggtggatca ggtgagcaaa cggcgagccg aagtgaggcg cgatgacagg attggaaaga
     4861 tttagaagat atttaccact tcactgttcc ttctttggtg gtgtcgatct cggcgaccgt
     4921 aggcacatcg ccgaagagat agcctttgat attgtcgaac aggaacgttt gcgcgtcggg
     4981 attggtgaga tcgagaccgt agtgattgat gatctgcgtc tgtttttgca gccaaccgct
     5041 ccagcacggc tggcagattt ctgccgcgat gcgctggccg agctcgttgg ggaaaggcgc
     5101 gaacggaagg gccgggtgct tctcaccgca gtgtgtgcat ttgattgtgt cagccatgag
     5161 ttctccttgg tcaataggac ctataggact tagtgtccga taaactttag tttgtcgcga
     5221 cattccgaca agctaaagct tatcggacat ctgcagcttt gattgccgca atattatcat
     5281 cgtccttcgg cttgaataag ccgatcgggc gcacgtcgcg gtggcgaatg attccacttc
     5341 catcgatcac gaagactgag cgcgccacgc gtcccggaat caacgaccgc gcgccgtaaa
     5401 gtgtcgatac tgcgccatcg gaatctgaca atagtcgcaa cggcagattg tgatgggccg
     5461 caaatttttt gtgcgactcg atcgaatcag acgagatgcc gaccacttca gcgccggttg
     5521 cgagatagtc gtcccagcga tcgcgcagcg aacacatctg tctcgtgcac accggcgttt
     5581 cgtcccccgg ataaaaaagc agcacgacga ctttgccgcg atgatcggac aagcgccact
     5641 cgccgccgtt tccatctttc agcgtaaagt cgggcgcagt tgtgttgacc gcagcgtcgt
     5701 tactcatgac tcaatcagct cggcgagtat gttaagcgct tcctcgactt cttcatcggt
     5761 gttgaagaaa tgtggcgaca tgcgcaggcc cgcgcttggc cgcgaatcga cgaaaactct
     5821 tctttcagcc aggcgttcaa ccatcgccgg cgcttcgttg acgccgatca taaccgatcc
     5881 gccgcgctgg ctcttctcgc gcggcgtgtt gatggtccaa ccacgctcgg acgcgaaatc
     5941 gatcatcaac tgagtgcggc gctctgattc ggcggcgatg gcagaaacgc cgacggcttc
     6001 gatgatctcc agtccgggaa tcgctgagta gagcgcggga atgctaggcg tgccttgcgc
     6061 gaagcgtcgc accgagcgcg cgtaacgcat gggcgcgtgt gcgaagtcga aaggtgaatc
     6121 gtgtgcgacc cagccggtga gtcgcggtcg cagatctctt tgcagatcgg ggcggacgta
     6181 caagtaaccg catgatggac caccacacag ccacttgatc gtgccgccga tcaggaagtc
     6241 gacgttccaa ccggcggcgt tgagttgtac gacaccggcg gattggtaga cgtcgagcaa
     6301 cacgagggcg ccttttgaat gtgcgtgctc gacgatcgcg cgggcggcga tacgatatga
     6361 gctgcaaaaa gacgtgtgcg cgagtgcgac gagggcggtt tcgttgtcga tcgcgtcgag
     6421 gatgcgctcg agtggcactg agatgttatc gcttgaagga acgacttcga cgcgcgcgcc
     6481 gatttgtcgc tgcgcatccc agaagtattc catcgaaggg aagtcgagcg ctgtcgtcac
     6541 gaccttgttg cggcctttgt tgaaatcgaa acacgaagcg acagtggcca tcgcgataga
     6601 tgcgtttggc tggatctgta ccgagccggc gtcagccgcg agaatcgtgg caatgcgatc
     6661 gccgacctgg cgcgatagtg tccaccagct cgtggcccat gcgtcttcgc tggtgtgatg
     6721 ttcccaggcg tcgatgtaac tgagcatcga ttcatgcgca gcacgcggca ccgggccgag
     6781 gctgtgactg agcagatgaa tgcccgaagc cagagttggg aattcatcac gatgcctgtt
     6841 tgcgatcata aatccatcca cagattacac agaaagaaaa taatctgcgg acccttctta
     6901 catcctgtaa tcctgtaaaa ctttctctaa accaagggcg tccttggata gattctcgaa
     6961 cacgcctttc gcttcatctc caatcggcgg atcgtgccgc tcttcgcgta tggcagccag
     7021 tatctcaccg gcgacgaatt ctttcgtcat cttcggcacg ttcgcgccct tactcatgtc
     7081 ggtgtcgatc gtcgtcggca tcacggtaat cacccgaacg ttgtgctcag acaagtacgc
     7141 tcgcatcgcc tggcccaacg acaacaacgc cgccttcgtc gcacagtaag tcccgacaat
     7201 cggcagatta actttcgcaa aagccgtggc cacattaaca atcacgccat cgcgctgtgc
     7261 gatcattccc ggcgccacgg cacgcgtcac ccgcaacacg ccgagatagt tcaccgcgat
     7321 ttcttcctgt acggcggcga agtccatcgt cagcggattg ccgaatcccg caatcccggc
     7381 gttgttaacg agaatatcaa tcgcgccgag gtgcacgagc tcttcgacgt cgtccgctat
     7441 cgtgacatcc aattgcaccg gcgtcacccg tgctgacttc aatgcttctc gatcttcagg
     7501 cttgcgcgcc ccggcaatca cctcgctcgc accagcatcg agaaacgccg tgacgagcgc
     7561 gcgcccaagc ccgcgactcg ctcccgtgac cagcactcgt ttgccttcaa tctgcatgtg
     7621 cgccaagttt cgcagcctca ccgccgcgct gcatgcgagc aagctgatac aaagccgctc
     7681 cgataactcc ccacaccacg atcaactcca cactcgtcaa cgactgtcca ctgatccgct
     7741 gcgagagcgt ctgcgtactc aacaccgccg catcctgtcg caactggata acgatcatca
     7801 gccccatcga caacagcgag acgattgcag ccgcctgctg caaacggtgc gacaggctga
     7861 tcttgatctc actcgaaagg gacgggttca gtcgagggag gagcaaaaat actaagctat
     7921 gaataaaata aagcacgacg agggcaaaaa ctgcaacgtt caacgcaagt gcaagctgtc
     7981 cgctgacgag caaaatcgca cagcagatga gcgtgagcgt cagcgcgcgt atcggcgtcg
     8041 cggtcgcttt gttgatgaaa ccgagccagc gcggcgcgag accgtcttcg acaaacatga
     8101 ttgcgagtcg tgagggaacg aagatcgtgc gattgagcgc ggtcatgatc gcggcgatcg
     8161 cgcctacagt aacaaacatc gccgcgcccg cgggtaaata gatcgatgcg acttcagcca
     8221 tcggcgcgct gctcgcttgc agtcgtccgc cgggcagcgc gccaaacgaa acgatcgaag
     8281 tgaaaacgta gatcagtgcc gtgatcgtga ttcccttcaa aagaatcttt ggcaaacgac
     8341 gcgtgctgtc tttcacttca ccggcggttt gcgcgatcga ctcgaagccg gcgtacgcaa
     8401 aaaagagcgg caacaggctc gcgccgaaac cactcggtcc gtgcgtgaaa aacggtacgt
     8461 agttttcgct atgcagcacg aataatccgg ggacgatcaa cacgagcagc gcgagacact
     8521 tcagtgcgga catcagtact tgaaatctac cgaaccagcg aatgccgatc acgtgaatca
     8581 agtaaaagac gacgagtgcg gcgagcgcga gaggcagtcg atacgttccg gcatcgatac
     8641 ggttgccgct gagggcaatg atgtaacccg ccatcgcgtt cgcaagatat gcgcccgcgc
     8701 cgatgtaaga aatgatcttg agccagcagc caatgtatgc gagtcgaaag cctcccacgg
     8761 ttcgcgagat gtgcagatac tcgccacccg gctcgcggcc gagtggtgtc gatagataag
     8821 cagcgtaagg gatcgacgtg gccagtatcg cgggtgtgag gacgagataa ccgagtatta
     8881 cgcttgggcc agtcagcgcc cacgcctggc tcgttacctg aaaaatgccc gcgccgatca
     8941 gaattccgat cacggtggca tacgattcaa ctgtacccag atcgcgacgc agtttcatga
     9001 gttattagcc accgattaca ctgattaatt acgcggattc aggactttct ccgcactttc
     9061 aacgccaacc accgggagct tcgttgaatc gatcaagccg agctgcgcaa gcactgacgc
     9121 ctggtcccaa tagatgtgct cgtgcgcaag cttgccgtca cggaagtgaa cgataacgac
     9181 gagcgccacc ttcacgtgtt tgcccgtcgg ctcaaggccg ggcaacatcc aatccatctt
     9241 aatcgagtgg gtaaactcaa acaccatctc atcaacaaca cgatcgacac cgatggtccg
     9301 cgacacgggc gtcatcgccg tgtcaggcgg catctgcgga atgaaacgtg tcgaatagaa
     9361 ctcgcgcagc tcatcgtgcc cgacgccgcc cgtcattacc ggcacgtgat tgacgtaagc
     9421 atcaacaacc atcgtctcga gcgtgtcgtc agtgttgcgg gtcgcgaatt catacttcac
     9481 gtgatcgtcc cagcgctgcg agagtatctg ttgtgctgaa gcgagaccta tccccgcgag
     9541 atgattgaca aagaatttga gcgaacggag attcgcgagc tctgcagcag cgaggtcgta
     9601 atgctcacca cccgtacgcg cgaaggcgtg atccgcgtca ggataatcgt gaatcgtggc
     9661 cttcgcatcg agcgccgcat gaatctgcgc ttgcgcttcc ggcgggcaaa acttgtcgag
     9721 cccggcgatg tgcagcagca acggactgtc caggttctcc gcctcgtcga gactcttctc
     9781 gatactgacg ccgtaataac caaccgcaca atcaggcttg tagcgcacgg acaacaggta
     9841 cgcgagtttt ccgcccatgc aaaaaccgac ggcgcccacg cgtccgctgc aggccgggtg
     9901 ctttcgcagc aactccatcg tcgcggcaga atcttcgacg gccttcgcct catcgatctg
     9961 ttgataaagc ccaaccgcgc gcttccaatc gtcgcccttg tcagtgagct cgatgttagg
    10021 ctggatgcgc cagaacagat ccggcacgag cgcgacaaaa ccgtgcgccg cataccagtc
    10081 agcaacgtcg cgcaggtact gattgatacc aaaaatctcc tggagtacga cgatgcctgg
    10141 tccataccca ctcgcaggca gcgccagata cccgtcgaac tccccaccat cgaaggattt
    10201 gattttgatc ataggtatcc acagattaca cagatcgcac agatgcagac aagaatcgac
    10261 ttactctgtg taatctgcgg aatctgtgga ccagttctcc gcgatccact tcttgcccgc
    10321 ctcgcacaca ctcagaaagt tgcacatgcg gcagtggggc gccgtcttga ccggaaaatt
    10381 cgccgggtcg gaagagaaga tcgttcgctg cattccttca tcgaccgcct gctcgcaacg
    10441 cgccggctcg agcagctcgt ccgcgagatg cacctcgaca ttcggatcga gaaaatgcag
    10501 cgtcacgcga acgttcgcgc ccgccagcga cggcagcagc tcgcgcactg caagcgcata
    10561 cgcctgcatt tgtaactggt aatcctcagc cgcagtcttc aaagcttctt ccagcgacgg
    10621 gtgacgattg accggctgcg gcgggccttc atcttcaaaa tcaaacgcga attgtccacc
    10681 cttcggcgcg cgaattcgcg gcgcgggcgg ctcgcgtgga gtttccgact tatcgtaaac
    10741 gacgcgattc gttttgaagt cgatgatctc gatctcagag tctgtaatca gcagcttgtc
    10801 gatgatgccg ttgagaactc cgagccggcg tcgcaaacga aagctcagct cactccacaa
    10861 accttcggca cccgccggac gcggcgagta gtgttcggcg atcttcggca cggcctcgac
    10921 gcgcttgtaa acatcactgg cgaggtaatt cttcgcgagc ggcatcatcg ctctgattgc
    10981 tcgttcgtga tcgatctcga cgaagcgatc cgaaagctgt gcctgtcgga gccttaagac
    11041 ttcttcaaaa ctcgtgcgca aacacccctc cacgctttga tccggcgtgt agcgctcaca
    11101 aaagcgatga atcactgagc cttttaaagt ggcagtcagg tttgacggcg gctcgggcgc
    11161 ttcagcattg ttccaaaccg ccatctggtc tgccgcgggt agttgcaaca cgcgatcgaa
    11221 ataatattga cgcggacagc gctggtagtt gatcaactgc gtgacactga aacgatgaat
    11281 cgcgttgccg gtttgctgac cgaacggctt gatcaaaggg aacgcctcgc ttaacgagtc
    11341 agccgaatgc atgctcgttt gcggcggttg ctccggttgc ggctcaaact cgtcgggcgg
    11401 ctcgtccgcg agattcatcg tgagctgtag ctgaacatcg tcatccagat cgatcaaatc
    11461 acttgcatgc ctgtcgtgca gctccaacga ttgccagatc cacttcaacc acgaatcgct
    11521 cttcgaagcg agactcgcga cgtcttcagt cacgcccgag aagatcaaac gatcttcagc
    11581 ccgcgtcgcc gcgacataaa gcagccgcat gctttcaaac tgctcgcgcc atgcgtgtcg
    11641 tttctcgaaa ttgctgaacg tgcaaccagc gacgagattg ccgcgaccat ccggcacttt
    11701 caacgtcagt ccctgatgac gatcgagcaa aacccagttg tcagtcgcga cccgagagag
    11761 tcgctgcaaa tcgggaatga tcacgactgg aaattcgagg cccttcgcct ggtgaatcgt
    11821 catcaatctg accgcgtccg ccgcttgatc tatttgtcct tcactctcgc gactgccgat
    11881 cgcttcaaac tcttcgacgt aacgcacaaa gtcgcgaatc aagtgcgttc ccgagctttc
    11941 aaaccgcgcc gcgagggtga acagtcgctc gtcattcgcg agacgctgcg cgccgtcgta
    12001 attagccgcg accacggtca ggtactccga ttcctcgacc gcgtaacgca gcaaatcctg
    12061 aatcggatag tgatgacgtc gatcgaccag ctcgccgatc aacttcgccg cgcgatcgag
    12121 cagctcgtgc tcttccggat tgatgaacgc gatctcctcg tgatgacgca gcgccgtgta
    12181 aagcgggcgc gtcgccgaga atccatgcac cgtctcgacc tcattcaaca gcggggcgca
    12241 gcgcagtcca agcaaagcgt tgtcagagag tccgcaaagc ggtgaacgca acactgccgc
    12301 gagcgcgatc tcgtcagtct tgttatctaa aaaacgcagc agttgaataa ggtcggtgat
    12361 ctcttcgcgc tcgtaaaaac cgcgaccaag cacggtctga tacggaatgt ttgcgcgacg
    12421 aaagaccgac tcataaatct gtacctgcgt catcgcacga aacaagagcg cgatgtcacc
    12481 gtacttgagt ttgtctggtc cctgcgtgag cgcgaggatg cgacgcgcaa gttgctgcgc
    12541 atcgagcacg cgcgagtcct gctccgcgcg cggatcgtct tcgagtgaag gcttcgtcgt
    12601 gaccaacagc tcgacgagtg gacccttcag tgaatcactg tcgcgcagct cgcgtttcgg
    12661 ttcgctcttt tcgtgtttca cgtagccaag ctggtccaga tttttatact cactcgcagg
    12721 aatgtcgtcc ggcacctgaa acagccgcgc gaagagatag ttgaagaacg cgatcagcgg
    12781 cggctggctg cggaagttca actgcaaagg cttctcttca ccgcgcgccg cgagcaggat
    12841 cgaggtcatc tcgcgaaaca cgtcaacgtc agcgccgcgg aaaccgtaga tcgactgctt
    12901 gcgatcgccg acgatgaaga gattcgcagg acggcgatct ttcgcgagcg cgagacgttc
    12961 gagcaacaca cgctgcacgg cattcgtgtc ctgaaattcg tcgacgagaa aaaagcggta
    13021 gcgctcagac gtgcgggcga cgacttccgg acgctgcaac agctcgagcg tgcgcaattc
    13081 gagatcgtca aagtcgagcg cggaaagttt ctgcttctcc tcattaagcc gctgatcgac
    13141 gcgcagcaac aagttgacca tctcgagcgc gtactgccgc gcaaacagat cgagacaaac
    13201 ctgcggcaca cgcccctgca gtcctttttc ccagacgagc gtgtcgagcg ctttcacgtg
    13261 atcggcaaca gacgctcgcg cttgcgggcg atgcgcacgg aaattctcga cgagacgaca
    13321 gaagtccgcc agcgtttcgt gcgacggaat cgacttcaaa agtttctgcg cttcggtcca
    13381 ggccgccagc acttcctcgc ggttcgtcat cgcggctttt gtcgtgcggc gcacagcgag
    13441 aaactcactc atcgtgcgtt cgagctcttc gagcgcggcg acgtgatctt cttcggtcgc
    13501 gtgtacccgg gccgtcgcga gcgcgagatc ttcgagggcg agcccctggc cacgcgcttg
    13561 gcgatagagt tgtgcgaggg ccgctgccaa acgactgcgc ccaaccccaa gagttaaccg
    13621 cgagatctcg acgtggccac tgctgatgaa ctcggagaga acttcctcga cgatcaattc
    13681 gagcatcatc gccgcgcgat gttcgtcgag taagagaaac tgcggatcga cgcgcgcctc
    13741 gatcggaaac tcgcgcagca gtcgcgcgca gaagccgtga atcgtcgtga ttactgcgcc
    13801 atcaagtgtt cgtttgtaat ttagccagcg acggcgctct tcttcgttgg cgatgcgcag
    13861 catctggttg agctcgctgc gcagccgttc gtgcatttca ttcgccgccc ggttggtgaa
    13921 ggtgatggcc acaatctgat cgatcgagag actgtgttcg cgaaggatgt ggacgtaacg
    13981 ctcgacgagc acggtggtct ttccagagcc cggccccgcc gtgacagaga tatgcttatc
    14041 gatctcgtac gcggctgcgg cttgttctgg taaaagtacg cgctgctcca taaaaaacca
    14101 tccacagatt tcacagattt tttgtctgtg taatctgtgt aatctgcgga ctacttcttc
    14161 ctttcaattc gatcgcgatc gtagcgacag acagctgagt agtcgcagaa gcggcatgtt
    14221 tttaggcgct cggatggatc gacgatgaag cgccccgatc ggatgccgtc gagaaattcc
    14281 cagatgcgtg ccgtcacttg ctcgcgaatt ctggtccagt cttcatcggt aaacaccgag
    14341 ttcttcgcgc gcaggttcag gtagtccaac tggctcgcac gatatagccc ttt
//
LOCUS       DASSFQ010000048         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_172120, whole genome shotgun sequence.
ACCESSION   DASSFQ010000048 DASSFQ010000000
VERSION     DASSFQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172120"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2699)
                     /locus_tag="VFH96_01585"
     CDS             complement(<1..2699)
                     /locus_tag="VFH96_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6972682.1"
                     /translation="MEPDQVSHYRILEKLGAGGMGEVYLAEDMKLGRKIAIKILSEEF
                     TTNKDRLHRFEQEASAASNLNHPNILTIHEMGIDEGRHYIATEYIDGVTLRRKMAASQ
                     LETHTILDIAVQVASALEEAHAAGIVHRDIKPDNIMVRRNGYVKVLDFGLAKLTETVD
                     RSPADGEAATRVLVHTDAGVVMGTSHYMSPEQARGKPVDARSDIWSLGVVIYELIAGR
                     TPFEGETSTDVIVAITQKEPPPLARFAPDVPAELEWIVMKALRKDRDERYQTIKELIT
                     DLKRLKQRLEFQTELERSAAPVSFTRSTISDFPGAPTTIAQQPVPTAEKTLSHVSSAE
                     YIVTGIKRNKLAAALIALVLIVVSASTFYFYKRKSHPLMTERDTVLLTDFVNTTGEPV
                     FDGTLKQALAVNLGQTPFLNLFPEDRVRETLRFMGRPPDDRITRDVGREICERQGIKA
                     MLTGSIVSLGSHYVITLEAVNPRSGDPIAREQIEAESKEKVLASLSTAAMNLRKKLGE
                     SLSSMQKYDVNIEQATTSSLEALKAYTMANDERAKGRSREALTLYQRAVDLDPNFAMA
                     YARIGVQYVNDEQLETAKEYVQKAYDLRNRVSERERLYIEEKYYSYITGEIDQTIETL
                     KTWVRLYPNDFIPHNNLSISYKMIGRNEDALREALEAVRLSPNNVAGHDNVVSGFMSL
                     GRFDEAEQAEREAQKINPDSLAAHVNNYHFAFLRQDQAAMDREVNWAKGKPEEAQFTA
                     MMSATALHNGKLKQSEELVKRAVEMFKQQNRRENASSILVGFANDLMYIGKCDQAKAD
                     AKAALDLLRGQMNLANSAAVYAACEDRDQAQKLLDEARAMYPKNTVINAIVPPIVTAG
                     IEEHRGNISQAIQLFESIRGYEGGMVLGLGTTFARGNLYLKQR"
     gene            complement(2767..>3059)
                     /locus_tag="VFH96_01590"
     CDS             complement(2767..>3059)
                     /locus_tag="VFH96_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET6972683.1"
                     /translation="RSGAVFRLIGFIISGLISIYILFITIRIIFSWGMISYTNRVMRF
                     LVDVTEPLLGPLRRTIPPLGWIDISPLVAILILWLFQQAVGGTLLSGPFRSF"
BASE COUNT          618 a          872 c          818 g          751 t
ORIGIN      
        1 atgcgctgct tgagatacaa atttccgcgc gcgaaggttg ttccgagacc aagtaccatt
       61 cccccttcgt atccgcgaat cgactcgaat aattgaattg cctgactgat attgccgcga
      121 tgctcctcaa tgcctgcagt cacaatgggc ggcacaatcg cattgatgac cgtgttcttc
      181 ggatacatgg cgcgcgcttc gtcgagcagc ttttgcgcct ggtcgcgatc ctcacaagcc
      241 gcgtagaccg ccgcgctgtt tgcgagattc atctggccac gcagtagatc cagcgccgct
      301 ttagcgtcgg ctttggcctg atcgcacttg ccgatataca tcagatcatt cgcaaacccc
      361 accaatatgc tcgacgcatt ctccctacgg ttttgttgct tgaacatctc aaccgcgcgc
      421 ttcaccagtt cttctgactg cttcagctta ccgttgtgca acgcagtcgc actcatcatc
      481 gccgtaaatt gcgcctcctc gggcttgcct ttggcccaat ttacttcgcg atccatggcg
      541 gcctgatcct gacgcagaaa ggcaaagtga tagttattga cgtgtgccgc taacgagtcg
      601 ggattgatct tctgggcctc tcgttccgcc tgctctgctt catcaaaacg tccgaggctc
      661 atgaaacccg agacgacatt gtcgtgcccc gccacgttat tcggactcag tcgcacagct
      721 tcgagcgcct ctctcaatgc gtcttcgttc cggccgatca ttttatagct tattgacagg
      781 ttgttatgcg gaatgaagtc gttcggataa agtcgcaccc aggtcttgag cgtttcgatc
      841 gtctgatcga tctcgcccgt gatgtatgag taatacttct cttcaatgta gagtcgctcg
      901 cgctcactga cgcgattgcg aagatcgtaa gccttctgca cgtattcttt tgcggtctcg
      961 agctgttcgt cgttcacgta ctgaacgccg atgcgcgcat aggccatcgc gaagtttgga
     1021 tcgagatcga ccgcgcgttg gtagagagtc aacgcttccc gcgatcgtcc tttcgcacgc
     1081 tcgtcgttgg ccatcgtata ggccttcagg gcttcgagtg acgacgtggt ggcctgctcg
     1141 atattcacgt cgtacttctg catcgagctc agcgattcac caagcttctt gcgcaggttc
     1201 attgccgccg tgctgagtga cgccagtacc ttttctttac tctcggcttc gatctgctcg
     1261 cgtgcgatcg gatcgccgga ccgcgggttc actgcttcca gagtgatcac gtaatgactg
     1321 ccgagactca caatcgatcc cgtcaacatc gccttgattc cctgtcgctc gcagatctcg
     1381 cgaccaacat cacgcgtgat gcggtcgtcg ggcgggcggc ccatgaagcg cagtgtctcg
     1441 cgcacgcgat cttcgggaaa cagattcaag aacggcgtct gtccgaggtt caccgcgagc
     1501 gcttgtttta acgtgccgtc aaaaaccggc tcgccggtgg tgttgacgaa gtcagtcagt
     1561 aaaaccgtgt cgcgctcggt catcagcgga tgcgacttgc gtttgtagaa gtaaaacgtg
     1621 gaggcgctca cgacgatgag cacgagcgcg atcaacgccg cggcgagctt gttgcgtttg
     1681 atcccggtga cgatgtactc agcgctcgaa acgtgactga gcgttttctc ggcagtcggc
     1741 accggctgtt gggcaatggt cgtcggcgcg ccagggaaat cgctgatcgt cgaacgtgtg
     1801 aaactgacag gcgccgcgga tctttccagt tccgtttgaa attcgagacg ttgtttgagc
     1861 cgtttcaaat ctgtgatgag ctctttaatt gtttgataac gctcatcgcg atccttacgc
     1921 agcgccttca tcacgatcca ttcaagctcg gcaggcacgt caggcgcgaa gcgagcgagt
     1981 ggtggcggtt ccttttgcgt aatggcgacg atcacgtcgg ttgacgtctc gccctcgaat
     2041 ggagtgcggc ctgcgatcaa ttcatagatc acgacgccga gactccagat gtcgctgcgt
     2101 gcatcgacgg gttttccgcg cgcctgctcc ggcgacatgt agtgagacgt acccatcacc
     2161 acaccggcgt ctgtgtgcac cagcactctc gtcgctgcct ctccatcggc gggactgcga
     2221 tcgacggttt cggtgagttt cgcgagaccg aagtccaaca ccttcacgta gccgttgcga
     2281 cggaccatga tgttgtcggg cttgatatcg cgatgaacga tgccggccgc gtgtgcttct
     2341 tcgagcgcgc tcgcgacctg gaccgcgata tcgagaatcg tgtgcgtctc gagttgtgag
     2401 gcggccatct tgcggcgcag cgtgacgccg tcgatgtatt cggtggcgat gtaatgccgg
     2461 ccttcgtcga tccccatctc gtgaatcgtg agaatgtttg gatgattcag gttcgacgcc
     2521 gccgaagctt cctgctcgaa acgatgtaga cgatctttgt tagtggtgaa ttcttcgctc
     2581 aggatcttaa tagcgatttt gcgtccgagc ttcatgtcct cggcgagata gacctcaccc
     2641 attccgcccg cgccgagctt ttcgagtatt cgataatgcg agacctgatc gggttccata
     2701 actgaggctc ttcatccaca gattacacag atttatttaa aatcatctgc gtaatctgtg
     2761 gatggttcaa aagctcctaa atggtccact caagagtgtt ccccccaccg cctgttgaaa
     2821 cagccacaga atcaaaatcg ctacgagcgg ggagatatca atccagccga gcggcggaat
     2881 cgttcgccgc agtggtccca gcaacggctc agtcacgtca accagaaaac gcatcacacg
     2941 gttcgtgtag ctgatcattc cccacgagaa aataatacgg atggtaatga acagaatgta
     3001 aatcgagatc aagccgctaa tgataaaacc gatcaggcga aacacggctc cgctgcgag
//
LOCUS       DASSFQ010000049         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_469895, whole genome shotgun sequence.
ACCESSION   DASSFQ010000049 DASSFQ010000000
VERSION     DASSFQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469895"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..2679)
                     /locus_tag="VFH96_01595"
     CDS             complement(25..2679)
                     /locus_tag="VFH96_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6972684.1"
                     /translation="MTHEAGTKLGRYEIRSKLGAGGMGEVYRARDEKLNRDVAIKVLP
                     AALSDDADRLHRFEQEAQAAGALNHPNILVIYDVGTNERAPYVVSELLEGESLRDRLD
                     HGPISSRKAIEYGTQIARGLAAAHARGIVHRDLKPDNLFITRDEHVKILDFGLAKLVQ
                     TTTEETAQTDVATRKVHTDPGAVMGTAGYMAPEQVRGRAVDHRADIFSFGAVLYEMLS
                     GRRAFRGDSAIETLNAILKEEPPAITDTNPNIAPALERVVWHCLEKTPEHRFQSASDV
                     AFALESLSGISTQASQQTLIRDAIPVRSRWTRERLVWLAVCLSLLAGVAALAIAHFSR
                     SEPNVRAVRLALTTPDKSTTPAHVTVSPDGTQVAFTASNAEGKRELWVRSLDADRAQP
                     LGGTEGALSPFWSPDSRSLGYFANGKLFKVEAARGRPQYLCDVRENAGGAWGREGVIL
                     FGGPEGLHRIAAQGGTPELATKIDAKEEAHRWPYFLPDGRHFIFLADAQTTENHHIRL
                     GSLDSPETQILFNAVTRVVYASPGYLLYVNQGALVAHPFDATGLKLTGDPITLAEHIA
                     EVGSNHEFDFSVSENGVLAYQIGGSKSQLVWFDRSGKKLETLGDPDNYAAVALSPDGR
                     RAVTGLLDADGRQSDLWLLDLSRGTKTRLTFNAQSDGDPLWSPDGKRLVFTSNRAGDG
                     HSHLYDMSVTASDDEQLLMQGDGDDIPTSWSRDGKYILFMRFQGGGRSGVWLLPLDDR
                     KPKPLLQSSAFELSAATFSPNGRFIAYTSDETGRNEVYVQPFPLSGEKWVISSGGGFL
                     PQWREDGKELFYVTTDGRVMSAEIKSEGAFDGLVTKPLFQIDIKRAPGYPYAVAPDGS
                     RFLVNTPADANNPVPMTVVLNWISTLKK"
     gene            2763..3140
                     /locus_tag="VFH96_01600"
     CDS             2763..3140
                     /locus_tag="VFH96_01600"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HET6972685.1"
                     /translation="MSKADTFAAGVVDAFRQAKIMGVRAGDEHRHTGVWVVVVEGRVF
                     ARSWNDKPAGWFQAFRKHASGAVQVGDLELPVRGRVVRSARIRDAVTATYGEKYPTKG
                     SRKWVEGFAEPERVVNTMEFIPG"
BASE COUNT          670 a          892 c          925 g          685 t
ORIGIN      
        1 ggccacggag ggccgcccct acagttattt tttcagggtt gaaatccagt taagcacgac
       61 ggtcatgggc acggggttgt tggcatcggc gggtgtgttg accagaaaac gtgagccgtc
      121 gggtgcgact gcatatggat agccgggcgc tcgcttgata tcgatctgaa agagcggctt
      181 cgtgacgagt ccgtcaaaag ctccttcaga tttgatctct gccgacatca ctctgccgtc
      241 ggtggtcaca taaaacaatt cctttccatc ctctcgccat tgcggcagga aacctccgcc
      301 ggaagagatc acccacttct cacccgacaa cggaaacggc tgcacgtaaa cttcatttcg
      361 ccctgtctca tcagatgtgt acgcgataaa acgaccattc ggcgagaagg ttgccgcact
      421 caattcgaat gcactcgact gcaaaagcgg tttgggcttg cgatcgtcca acggcaacag
      481 ccacacgccc gaacgaccac caccctgaaa gcgcatgaac aaaatatact ttccatcgcg
      541 cgaccaactc gtcggaatat catcgccgtc gccttgcatc aatagctgct cgtcgtcgct
      601 ggcggtcaca gacatatcgt aaagatgact gtgaccatca ccggcgcgat tggacgtgaa
      661 cacgaggcgc ttgccgtcag gcgaccagag tggatcgccg tccgactgcg cgttaaaggt
      721 caatcgtgtt ttggtacctc gtgaaagatc gagtagccaa agatcactct ggcgcccgtc
      781 tgcatcgagt agaccagtca cggctcgtcg cccgtcaggt gaaagcgcga ctgccgcata
      841 gttgtcagga tcaccgagtg tctcgagttt cttaccgctt cgatcaaacc aaaccaattg
      901 cgatttggag ccgccgatct ggtaagcgag tacgccgttc tcagaaaccg aaaaatcaaa
      961 ctcgtgatta gatccgactt ccgcgatgtg ttcggcaagc gtaatcggat cacctgtcag
     1021 ttttagccca gtcgcatcga acggatgggc taccagcgcg ccctggttca cgtagagcag
     1081 gtaacccgga gacgcataaa cgacacgcgt tacagcgttg aagagaatct gagtctccgg
     1141 tgaatcgagt gagccgagac gaatgtgatg gttttcagta gtctgtgcat cagcaagaaa
     1201 gatgaagtga cgaccatccg gcaggaaata gggccagcga tgcgcttcct ctttcgcatc
     1261 gatcttggtc gcgagctcgg gtgtgccacc ctgggcagcg atgcgatgca aaccttctgg
     1321 tccaccaaag agaatcactc cctcacggcc ccacgctccg cccgcgtttt ctcgaacatc
     1381 acacaaatac tgcggacgcc cgcgcgcagc ctcgactttg aagagttttc cgtttgcgaa
     1441 atagccgagc gaacgactat ccggtgacca gaatggcgag agtgcgcctt cggtgccgcc
     1501 caacggctgc gcgcgatccg catcgagtga acgcacccaa agctcgcgct tcccttcggc
     1561 gttgctggcg gtgaaagcga cctgcgtgcc gtcaggcgag acggtgacgt gcgccggagt
     1621 tgtcgacttg tccggcgtgg tgagcgcgag tcgcacggcg cgcacgttgg gctctgaccg
     1681 tgaaaaatgt gcgattgcga gtgctgccac gccggccagt agtgataaac atacagccag
     1741 ccagacgagt cgttctcttg tccaacggct ccgcaccggg atagcatcac gtatcaacgt
     1801 ttgctggctc gcctgtgtgc tgattcctga aagcgactct aacgcaaacg cgacatcact
     1861 cgctgattga aaccggtgct ccggagtttt ttcgagacaa tgccagacga cgcgctcgag
     1921 cgccggagcg atgtttggat tcgtgtctgt gattgcgggc ggctcttcct tgaggattgc
     1981 gttgagcgtt tcgatcgcag agtcgccgcg aaaggcacgc cggcctgaca gcatttcgta
     2041 gagcactgcg ccaaacgaaa aaatgtcggc gcgatggtcc actgcgcgcc cgcgtacttg
     2101 ttccggcgcc atgtaacccg ctgtgcccat caccgctccc ggatcggtgt gtactttgcg
     2161 tgtagcgaca tcagtctgcg cagtctcttc tgttgtagtt tggaccagtt ttgcgagacc
     2221 gaagtcgagg atctttacgt gctcgtccct ggtgatgaag agattgtcgg gtttgagatc
     2281 gcggtggacg atgcctctcg catgcgcggc cgcaagcccg cgcgcgatct gcgttccgta
     2341 ctcgattgcc ttgcgactcg aaattggccc atggtccaaa cgatcgcgca ggctctcgcc
     2401 ttcaagcagc tccgaaacga cgtaaggcgc acgctcgttt gtgccgacgt cgtagatgac
     2461 gaggatgttc ggatgattca gcgcgcccgc agcctgcgct tcctgttcga agcgatgtaa
     2521 acggtcggcg tcgtcagaca gcgcggccgg caggactttg atcgcgacat cgcgattgag
     2581 cttttcgtcg cgcgcgcgat agacttcgcc cattccaccc gcgcccagct tcgagcggat
     2641 ttcgtaacgg ccgagtttcg taccggcctc gtgagtcatt gttgggcgcg attctattac
     2701 gactcagccg gatttgcatg gattaatcct gattattttg tatattgaac accgaggtcc
     2761 atatgtcaaa ggcagacaca ttcgcggccg gcgtcgttga tgcgtttcgc caggccaaga
     2821 tcatgggcgt tcgtgccgga gacgagcatc gccatacagg agtgtgggtt gtagtagtgg
     2881 agggcagagt ctttgcccga tcgtggaacg ataaaccggc tggatggttt caggcgtttc
     2941 ggaagcatgc gagtggagcg gtccaggttg gtgatttgga actccctgtt cgcggccggg
     3001 tagttcgcag tgcacgtatt cgggatgcgg tgacagccac gtacggagag aaatacccga
     3061 cgaaaggctc gcgcaagtgg gtcgaaggat tcgccgaacc ggagcgagtc gtcaacacga
     3121 tggagttcat accaggttga ggtcgcaact gtaggggcgg ccctccgtgg cc
//
LOCUS       DASSFQ010000050         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_916421, whole genome shotgun sequence.
ACCESSION   DASSFQ010000050 DASSFQ010000000
VERSION     DASSFQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916421"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..871)
                     /locus_tag="VFH96_01605"
     CDS             complement(209..871)
                     /locus_tag="VFH96_01605"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HET6972686.1"
                     /translation="MRKLACLVLLTVLAAGCHGKFSSQIKGSGKRELQKREVAAFTSI
                     STEGAFTIEVTCQKNQSLEVEGDDNVLEYVTTEVGSNILRLKNTRNYSTSEPVKFKIS
                     VPNLEGLSVNGAGHVDVKGLNNDKFEIDTNGAATIVVSGNTKLIDVGANGAGKIDAEN
                     LRAPRAVVDSRGVARIDLGVTDQLDVTVSGPSTVTYKGDPVMNKTIHGPGKVERRGSE
                     GA"
     gene            complement(884..2470)
                     /locus_tag="VFH96_01610"
     CDS             complement(884..2470)
                     /locus_tag="VFH96_01610"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HET6972687.1"
                     /translation="MKLLIAIGIIVVATIHGYGAAWLAIWMLFHPYQSVKLLGITIWP
                     QGMIPRHREKLAQSIGNAVGNELVSQQTVFDALFETDFFQRKVEDFVGAYTNELLSRV
                     YPSFIDALPSQARAPILDTISALQYRLAEYIAEMLKSEETANAIEHFVDKQVDELLDR
                     RLGDMVSDSSLDHVLQFAEDQVHKLFATEGFETKVHEFVSGRMDDIARSNATLADTFT
                     PETIAFIKDRIDQQVPPIVHHLAEIATAQTTRKQIGGLIKLEVDEYYQQLSLFKKIFV
                     SRERIHREVDELMNKTLPRRVEEYLRGPAFEQEAEAFLNATIDKVMARPLNELIGQFD
                     SIRFEDLKSQISNRLVEILRSEALSTSVSMQVEETLDRLRPQTLGALLQQLDANSTTH
                     AKEFLTKSLLSLLSRDDTARTINAIMSTQIERLLISPIGRLGDHVSRNAMERASGALV
                     ERITLAARERLPVAIAEFDVGGLVRKKVSDYPLEKLEDLVLSVARDQLKTIEIFGAVI
                     GFFIGLAQAIYFWFTWVPGR"
     gene            complement(2573..>2900)
                     /locus_tag="VFH96_01615"
     CDS             complement(2573..>2900)
                     /locus_tag="VFH96_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6972688.1"
                     /translation="LPVDVLPQVSVAELNERLANNDMQIVDVRRPAEYENGHVPHAQN
                     APLASLDKSIGPLQLEKDKLTAVICAGGYRSSAAASLLQQQGFSNLLNVTGGTGAWIN
                     AGYPTE"
BASE COUNT          632 a          776 c          787 g          705 t
ORIGIN      
        1 aaatgcttca acaggacaaa cggctgcgca gtcggtgtac ttgcaatcga cgcacaattc
       61 aacaactacg taggtcattc gggaactagg ctcctttcga tgatggcaaa agttattcag
      121 tagcgagtgt aatgtcaacc aaaaagagct ttatccacag attacgcaga ttgtttatga
      181 catctgtgta atctgtggat aaagaagctc aggcgccttc actgccgcgg cgctcgactt
      241 tgcccgggcc gtggatggtt ttgttcatga ccggatcgcc tttgtatgta actgttgaag
      301 gtcccgagac ggttacatcg agctggtcag taacaccgag atcgatcctc gcaacgcctc
      361 tgctgtcgac cacggcacgc ggggctcgca gattctccgc atcgatcttg ccggcgccgt
      421 tcgcaccaac gtcgatcaac ttcgtgttcc ctgaaaccac gatcgtcgcg gcgccgttgg
      481 tatcgatttc aaacttgtcg ttattcaatc ccttgacgtc gacgtgtccg gcgccgttga
      541 cggaaagacc ttcgagattc ggaacagaga tcttgaactt caccggctca gaggttgaat
      601 aattcctggt gtttttgagc cgcaggatgt tgctgccgac ctcagtcgtg acgtactcga
      661 gcacattatc gtcaccttca acctcgagac tctgattctt ctgacatgtg acttcgatcg
      721 tgaacgcgcc ttcggtcgag attgatgtga acgctgcgac ctcgcgcttc tgaagctcgc
      781 gcttgcccga gccttttatc tgagagctga acttgccatg acagccagcc gccaacaccg
      841 tcagcaaaac caagcacgct agttttctca ttacagttct cctttatcgt ccgggaaccc
      901 aggtgaacca gaaatagatc gcctgcgcga gaccgatgaa gaaaccaatc accgcgccaa
      961 agatttcaat tgtcttcaat tggtctcgcg ccactgaaag cacgaggtct tcgagtttct
     1021 caagaggata gtctgatacc ttcttgcgca ccagcccgcc aacgtcaaat tcggcaatcg
     1081 ccaccggcag tcgctcgcgc gccgcgaggg tgatgcgctc gacgagagcc ccactcgcac
     1141 gctccatcgc gttacgcgaa acgtgatcgc cgagacgccc gatcggagag atcaacaggc
     1201 gctcgatctg cgtgctcatg atcgcattaa tcgtgcgtgc ggtgtcgtcg cgtgaaagca
     1261 ggctgagcag actcttggtc aaaaactctt tcgcgtgggt ggttgagttt gcatcaagct
     1321 gctgcagcag cgcaccgagt gtttgtggac gcagccgatc gagcgtttct tcgacttgca
     1381 tcgatactga cgttgacaaa gcttcactgc gaagtatctc gacgagtcga ttactgatct
     1441 gcgacttcag atcttcgaaa cgaatcgagt caaactggcc gatcagctcg ttgagcggac
     1501 gcgccatcac cttgtcgatg gtggcattca aaaacgcttc cgcttcctgt tcgaaggcgg
     1561 ggccgcgtaa atattcttca acgcgtcgcg gcagcgtttt gttcattagt tcatcgactt
     1621 cgcggtgaat gcgctcgcgc gagacgaaga tctttttgaa gagcgagagt tgctggtaat
     1681 actcatcgac ctcgagcttg atcaacccgc cgatctgttt gcgtgtcgtt tgggcggtgg
     1741 cgatctccgc gaggtgatgg acgatcggcg gcacttgttg atcgatgcga tctttgatga
     1801 acgcgatcgt ttcgggcgtg aacgtgtctg cgagtgttgc attgctgcgc gcgatatcgt
     1861 ccatgcgacc ggagacaaac tcgtgtactt tcgtctcgaa tccttccgtg gcgaacagct
     1921 tgtgaacctg gtcttcggcg aactggagga cgtggtcgag tgagctgtca ctaaccatat
     1981 cgccgagacg gcgatcgagt agctcatcaa cctgtttatc gacgaagtgt tcgatcgcgt
     2041 tcgcggtctc ttcgctcttc aacatctcgg cgatgtattc ggcgagacga tattgaaggg
     2101 cggagatcgt gtcgagaatc ggcgcgcgtg cctgcgatgg aagggcgtcg atgaacgacg
     2161 ggtagacacg gctgagcagc tcgttggtgt acgcgccgac aaagtcttcc accttgcgtt
     2221 gaaaaaaatc agtttcgaag agcgcgtcga agacggtttg ttgtgaaacg agctcgttgc
     2281 cgacggcgtt gccgatcgac tgggccagct tctcgcgatg gcgcggaatc atcccctgcg
     2341 gccagatcgt gatgccgaga agttttactg attggtacgg gtgaaagagc atccagatgg
     2401 cgagccacgc cgcgccgtag ccgtgaatcg tcgcaaccac aatgatgccg atggcgatta
     2461 aaagcttcat agacacttag acaggattta caagattcta caggattaca agatacttgt
     2521 tttgtaaacg tcttggttta tcctgaaaaa tcttgtaaat cgtgtctaaa acttattctg
     2581 tggggtagcc ggcgttgatc cacgcacccg ttccgccggt cacattgagg agattcgaga
     2641 acccttgttg ctgcaacaaa cttgccgccg cgctcgatcg ataaccaccg gcgcagatga
     2701 cggcagtcag tttgtctttc tcgagctgca gtggaccaat actcttgtcg agactcgcca
     2761 gcggtgcgtt ttgtgcatgc ggtacgtggc cgttctcgta ttccgcgggg cgtcgtacat
     2821 cgacgatctg catgtcgttg tttgcaagac gctcattcaa ctccgcgaca ctcacttgcg
     2881 gaagcacatc aaccggcaac
//
LOCUS       DASSFQ010000051         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_767368, whole genome shotgun sequence.
ACCESSION   DASSFQ010000051 DASSFQ010000000
VERSION     DASSFQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767368"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFH96_01620"
     CDS             <1..1307
                     /locus_tag="VFH96_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET6972689.1"
                     /translation="HREIGALEDEYQQVQAAIRKSSPQYSALTQPQPLDLAGIQQQLD
                     QDTTLLQYALGEERSYVWAVTRNSLQAYVLPKRADVEIVAREVYESLTARSEVKSLES
                     PVQRRTRIALADANFEQAAVKLSAMALAPAVAQLTRKRLVVVADGALQYVPFAALSVS
                     KGSYQPLIIEHEVVSLPSASAFAVQRQNLKDRALAPKSVAVVADPVFSTNDARFKNGA
                     LGQTASEPTTTRIIEHLQGTAKGQLSIPRLPFTRQEADQILAVAPSGFKALDFNASRS
                     IATDGELSKYRYVHFATHGYLDTSRANLSAIVLSLFDAQGKPQDGFLRTHDIYNLKLP
                     AELVVLSACETGLGKDVTGEGLEGLTRGFMYAGARRVIVSLWNVNDKATASLMQRLYV
                     GMLRSGKTPAAALRAAQIEMLRTRQWQSPYFWAPFVLQGEWK"
     gene            1304..>2139
                     /locus_tag="VFH96_01625"
     CDS             1304..>2139
                     /locus_tag="VFH96_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972690.1"
                     /translation="MKSILALVLLTLLAGSVLVAQNPDDMTRKLWDTAFVGSSPKKTT
                     RRRVNSYRIATPNVPTNNVAPETVVGVTLWRLRPARPSDSGERLIVHEDNADAEWLPE
                     RISASTRLVQGDKLRISVEAVRGGYLYVIDREQYADGSLGEPYLIFPTKRTAGGDNHV
                     GIGRLVEIPAQDDNPPYFTMKKSRADHVAEVLSVIVTPTKLEGVEITDKALKLTDAQV
                     LAWEKAWGSNVGNLEMSTAGQAWTKEEKDARTRALTASAPAPQLVFYRPGIKTTDSLL
                     VK"
BASE COUNT          479 a          577 c          632 g          451 t
ORIGIN      
        1 tgcatcgcga aatcggcgcg cttgaagatg agtatcagca ggtccaggcc gcaattcgga
       61 agagcagtcc gcagtactcg gcgttgactc aacctcagcc gctcgatttg gctgggatcc
      121 aacaacagtt ggaccaggac acgacgctgc ttcaatacgc gcttggcgaa gagcgcagtt
      181 acgtttgggc cgttaccagg aattcattgc aggcatacgt actaccgaaa cgagcggatg
      241 tggaaatagt tgcgcgcgag gtctatgaat cgctcacggc gcgcagcgaa gttaagtcac
      301 tggaatcacc tgtacaacga aggacgcgca tcgcgctggc cgacgcgaat ttcgaacagg
      361 cggcagtgaa gttaagcgcg atggcactcg cgccggctgt ggctcaactc acgcggaagc
      421 gattggttgt ggttgcagat ggcgctttgc agtatgtgcc attcgcggcg ttgagtgttt
      481 ccaagggctc ctaccagccg ctcattattg aacacgaggt tgtcagcctg ccgtctgcct
      541 cggccttcgc ggttcaacgt cagaatttga aagatcgtgc gctcgcgccg aagtcagtcg
      601 ccgttgtcgc cgatccggtg ttttcaacca acgatgcgcg gttcaaaaac ggcgcgctag
      661 ggcagactgc gtcggaacca acaacgacaa gaatcatcga gcacttgcaa ggcactgcta
      721 aagggcaact atcaattcca cgtttgcctt tcactcgcca ggaagccgat caaattctcg
      781 ccgtcgcgcc ttccggtttc aaagcactcg atttcaatgc aagccgatcg atagctaccg
      841 atggcgagct cagcaaatat cgttacgtgc acttcgccac gcacggctat ctcgacacgt
      901 cgcgcgcgaa tctatcggcg atcgtactct cgttgttcga cgcacaggga aaaccgcagg
      961 acggatttct ccgcacgcac gatatctaca atctcaaatt gccggcggag ttggtcgtgc
     1021 tgagtgcgtg tgagacgggt cttggtaaag acgtcaccgg tgaaggtctc gaagggttaa
     1081 cgcgcggatt catgtatgcc ggcgcgcgtc gtgtgatcgt gagtttgtgg aatgttaacg
     1141 acaaagccac cgcttcgttg atgcaacgtc tttacgtggg aatgttgcgc agtggcaaaa
     1201 cacccgcggc ggccttgcgc gccgcacaga tcgagatgct gcggacacgg caatggcagt
     1261 cgccgtattt ctgggcgccg tttgtgttgc agggagagtg gaaatgaaat caattttagc
     1321 cttggtcttg ctaacgctgc tcgcgggttc cgttttggtt gcgcaaaatc cggacgacat
     1381 gacgcgcaaa ctgtgggaca ccgcttttgt gggctcctcg ccaaagaaaa ccacgcggcg
     1441 gcgggtgaat agttatcgca tcgcgacacc gaatgttccc accaacaacg tcgcgcccga
     1501 gaccgttgtt ggcgtcacgc tctggcgtct gcggccggcg agaccatctg attcaggcga
     1561 gcgtttgatt gttcacgaag acaatgctga cgccgagtgg ttgccggaac gaatttcagc
     1621 gagcaccaga ctggtccagg gcgacaagtt gcgcatcagc gtcgaagccg tgcgtggtgg
     1681 ttatttgtat gtgatcgatc gcgagcagta tgcagacgga tcgcttggcg agccttatct
     1741 gatctttccg acgaagcgga ctgccggtgg cgataatcat gttgggatcg gcaggctcgt
     1801 ggaaataccg gctcaggacg acaatccgcc gtactttacg atgaagaaga gccgtgcgga
     1861 tcacgttgca gaggttttga gcgtgatcgt gacgccaacc aagctcgaag gcgtggagat
     1921 caccgacaaa gcgttaaagc tcaccgacgc tcaagtgttg gcatgggaaa aagcctgggg
     1981 ctcaaacgtc ggtaacctgg agatgtccac cgccggccaa gcatggacca aggaagaaaa
     2041 ggacgcccgc acccgcgccc tgactgcgag cgccccagcg cctcaattag tcttttaccg
     2101 tcccggcatc aagacaacgg attctttgct ggtcaagtt
//
LOCUS       DASSFQ010000052         4507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1354676, whole genome shotgun sequence.
ACCESSION   DASSFQ010000052 DASSFQ010000000
VERSION     DASSFQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354676"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFH96_01630"
     CDS             <1..658
                     /locus_tag="VFH96_01630"
                     /inference="COORDINATES: protein motif:HMM:NF028180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972691.1"
                     /translation="KWKQTSRLVMLLPHGYEGQGPEHSSARLERYLQLCAENNLQVCY
                     PTTPAQYFHLLRRQVRPGMERPLVVMTPKSLLRLPAAASSIEELASGGFQPLIDDAEI
                     ADREAVKRIVLCSGKVYYDLAEARKKSDDRSVSIVRLEQFYPFPSISLRDTLAQYPNA
                     KELVWAQEEPQNMGGWTFVRDRLENPRYVGRSASASPATGSYSIHQKEQAEIVTQALE
                     "
     gene            822..2078
                     /locus_tag="VFH96_01635"
     CDS             822..2078
                     /locus_tag="VFH96_01635"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HET6972692.1"
                     /translation="MTKRIIAIVFIFICTSVAWAILGATLFQRTYDSGSVSSDRVESL
                     WGTAQNQAPPSASFKTTTTKKQAAIEAGATVSKTISEDHITELPLESSKIDVDLDLQH
                     RQKGLLWYSTYKVKFSGVYGFRNTSDKEQTVDFKLPFPTAQAIYDDLIFTVDGNNVPL
                     TNEQNSAVTAFKVAAGKTAQLAVSYSSQGLSQWRYRFGDGEVAQVRNFSLRMKTNFKD
                     IDFPDNTISPTEKTETGDGWDLNWSYKSLLSGYQIAMVMPEKLQPGPLAGRISFFAPV
                     SLFFFFFLMLIITTMRGIDLHPMNYFFLAAAFFSFHLLLAYLVDHISIHAAFAVSSLV
                     SVALVVSYLRLVVGIGFASREAALAQIIYLVLFSYAFFLKGFTGLAITIGSILTLFVV
                     MQITGRVRWTERFGRSSTTQPAADLS"
     gene            2182..3423
                     /locus_tag="VFH96_01640"
     CDS             2182..3423
                     /locus_tag="VFH96_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972693.1"
                     /translation="MKAILSFYSDALKIALQSIFAHKLRAFLTLIGIIIGVASVVTVG
                     ASISGLNTYVIERISKVLGSNHFMIARMAFSGHMDDEEFERRNRRNKRLNWDDYEWVR
                     DHCPSCSEVGAGVGAGTDLRQDGIEFPGAQVFGHSANMVDIEDKTIEFGRFISEDEVQ
                     RSALVVVLGADIKEKFFPNQDALGKTLKVRGLPMRVVGVEEKRGSFFGDSFDRHIYIP
                     VTTHLQIFGRNGLQIHGKTRNRENFHAAIEDARVAMRIKHRLNGNEEDDFGLVNVEEL
                     NNQIDEFTGAIAKVVIPITFITLVVGGIVVMNIMLVSVTERTFEVGLRKAVGATKRQI
                     LMQFLIESGILCALGGVIGLLVSWGITTLITTLASITMTITIGYIILALGVSSIVGMI
                     AGIYPAFKAARLDPILALTKA"
     gene            3423..>4507
                     /locus_tag="VFH96_01645"
     CDS             3423..>4507
                     /locus_tag="VFH96_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972694.1"
                     /translation="MKLRLPQGILRETLKMAIDSVRGHKFRSFLTVLGIVIGVMTAIC
                     IASLLTGLRQNIVAMIEEYGTNNIYAFHLSTGPRTSEDRSERLRRPLTVADADAIKAA
                     SAVEDVAHVAPNVGYGGGQFDDNVTYQGRNYRWGNTSGVSPNYADITNISLREGRFIT
                     EADDSQRSNVMVIGVNAAEALFPGYNQIAGAQVRMGGYNFEVIGVLEKRKAGFFGENE
                     EDNAVYIPFRTAQKIAPAKGYMLLVIRGRTGKVQEALAQSEEILRRRRHVKFDEPNNF
                     DIKTADKFIEQFDSITAMVGLIAIAISSLGLLVGGIGVMNIMLVSVTERTREIGVRKA
                     IGARRRDIVRQFLFEAMMLTFLGGVLGV"
BASE COUNT          990 a         1255 c         1262 g         1000 t
ORIGIN      
        1 caagtggaag cagacctcgc ggctcgtgat gctcctgccg cacggttacg aaggacaagg
       61 accggagcat tccagcgcgc ggctcgagcg atacctgcaa ctctgcgcag agaacaatct
      121 acaagtctgt tatccgacga cgcccgcgca gtatttccat ctgctgcggc ggcaagtcag
      181 gccgggaatg gaacgtccgc tcgtcgtgat gacgccgaag agtctgctgc gattgccggc
      241 agcggcgtca agcatcgagg agctcgcgag cggaggtttt cagccgctga tcgacgatgc
      301 ggaaattgca gatcgtgagg cggtgaagcg aatcgtgctg tgcagcggca aggtctacta
      361 cgacctcgca gaagcgcgaa agaagagcga cgatcgaagt gtttcgatag tcaggctcga
      421 gcaattttat ccgttcccct caataagcct gcgagacacg cttgctcagt atccgaacgc
      481 gaaagagctg gtttgggcgc aggaagagcc gcagaacatg ggcggctgga ccttcgttcg
      541 agatcgactc gaaaacccga gatacgtggg acgctcagcc tccgctagcc ccgccaccgg
      601 ctcctacagc atccatcaga aagaacaagc cgagatcgtc acccaagcgc ttgaatagaa
      661 cttattttta gccgcggata aacgcggata aacgcggatc agaacttttt ctgtttttct
      721 gatccgcgtt tatctgtgtt catccgcggc taaatccaca tacgtttgct gtggctcacc
      781 acattgcccg ccggccgctc caggcccaaa atccccagac tatgactaag agaatcatcg
      841 ccatcgtgtt catcttcatc tgcacgtccg ttgcctgggc catcctcggc gccaccctct
      901 tccagcgcac ctacgattcc ggctccgtgt cttcagaccg ggtcgaatcg ttgtggggta
      961 cggcccagaa tcaggcccca cccagcgcgt cgttcaaaac aaccacgacg aaaaaacaag
     1021 ccgcaatcga ggcgggcgcc actgttagca aaacaatcag cgaagaccac ataaccgagc
     1081 tgccactcga gagtagcaag atcgacgtcg atctcgatct tcaacatcgc cagaaaggac
     1141 tgctctggta cagcacttac aaagtcaaat tcagtggcgt ctatggcttc cgcaacacga
     1201 gcgacaagga acaaactgtc gatttcaaac ttccgttccc caccgctcag gcgatctacg
     1261 acgaccttat cttcactgtc gatggcaaca atgtgccact gactaacgaa cagaactccg
     1321 ctgtcaccgc attcaaagtc gccgccggca aaactgccca gctagccgtc agctacagct
     1381 cgcagggact cagccagtgg cgttatcgtt ttggcgatgg cgaggtggcg caggtccgca
     1441 atttctcgct gcgcatgaaa acaaacttca aagacatcga tttccccgat aacacgatct
     1501 ctccgactga aaaaacggaa accggcgatg gctgggatct caactggtct tacaagagtt
     1561 tgctgtctgg ataccagatc gcgatggtca tgccggagaa gttgcagccc ggtccactcg
     1621 cgggacgaat cagctttttt gccccggtat cactcttctt ctttttcttc ctcatgctga
     1681 tcatcacgac gatgcgcggg atcgatctgc acccgatgaa ctactttttc ctcgccgcag
     1741 cgttcttctc cttccacttg ctgctggctt atctcgtcga tcacatctcg attcacgcgg
     1801 ctttcgcggt gtcgtcgctc gtgtctgttg cgctggtcgt gagttacctg cgtctcgtcg
     1861 tcgggatcgg cttcgcgagc cgggaagcgg cgctcgcgca gattatctat ctcgtgttgt
     1921 tttcctacgc gtttttcctc aagggattca ccgggctggc catcacgatc ggatcgatat
     1981 tgacgctttt tgtggtcatg cagatcacgg gtcgcgtgcg ctggacggag cggtttggac
     2041 ggagctcaac cactcagccc gctgcggatc tttcctgagc tgttaataac acggcggtgt
     2101 ctaacgtctt acccgtgtaa acccgaagct tcaatcgaac ttcacgggtt tacacggcgt
     2161 atacgccccc gactccgtgt tatgaaagcg atactttcct tttactccga tgcgctgaag
     2221 atcgccctgc agtcgatctt tgcccacaag ctacgcgcgt ttttgacgct gatcggcatc
     2281 attatcggcg tcgcgtcggt ggtgacagtc ggagcctcca tcagcggact caacacctac
     2341 gtcatcgaga gaatcagcaa agttctcggc agtaatcact tcatgatcgc ccgcatggcc
     2401 ttctccggtc acatggacga cgaagaattc gagcgccgca atcgacgcaa taaacgactg
     2461 aattgggacg actacgagtg ggtccgcgac cattgtcctt cctgctctga agtgggcgcg
     2521 ggtgtcggcg ccggaaccga tctgaggcag gacggtatcg agttccctgg cgcgcaggtc
     2581 ttcggccact ccgccaacat ggtcgatatc gaagacaaaa ctatcgagtt cggccgcttc
     2641 atctcggagg acgaagtgca acgctcggcg ctcgtcgtcg tgcttggcgc cgatattaaa
     2701 gagaagtttt tcccgaacca ggatgcgctt ggaaagacgt tgaaggtgcg cgggctgccg
     2761 atgcgcgtcg tgggcgttga agagaagcgc ggctcgtttt tcggcgactc cttcgaccgt
     2821 cacatctaca ttcctgtcac cacgcacctt cagatcttcg gacgcaacgg cctgcagatt
     2881 cacggcaaaa ctcgcaaccg cgagaatttt cacgccgcga ttgaagatgc gcgcgtggcc
     2941 atgcgcatca agcatcgact caacggcaac gaagaagatg attttggtct ggtaaatgtt
     3001 gaggaactga acaaccagat cgacgagttt accggcgcga tcgctaaagt tgttatcccg
     3061 attactttta ttaccctcgt cgtgggtggc attgtcgtaa tgaacatcat gctcgtgagc
     3121 gtgacggaac gaacgtttga agtcggactc agaaaagccg taggcgccac gaagcgacag
     3181 atcctgatgc agtttctgat tgaatcggga attctttgtg cactgggcgg tgtcatcgga
     3241 ttgttggtgt cctggggcat cacgacgctg atcaccacac tcgcatcgat cacgatgacg
     3301 atcacgatcg gttacatcat tttggcgttg ggcgtgtcca gcatcgtcgg catgattgcg
     3361 ggcatttacc cggcattcaa ggccgcgcga ctcgatccca ttctggcact gacaaaggcg
     3421 tgatgaagct tcgattgcca caaggtatat tgcgcgagac gctgaagatg gcgatcgaca
     3481 gcgtgcgcgg tcataagttc cgcagctttc tgacggtgct cggaatcgtg atcggcgtca
     3541 tgactgcgat ctgcatcgcg tcgctactta ccgggctgcg ccaaaacatc gtcgcgatga
     3601 tcgaggagta tggaacaaac aacatctacg cgtttcatct ttccactggt ccacgcacga
     3661 gtgaagatcg ctctgaacga ctccggcgac cgctgacagt cgctgacgcg gacgcgatca
     3721 aagccgcgag tgcagttgaa gatgtggctc acgtcgcgcc gaatgtcggt tacggtggtg
     3781 gtcaattcga cgacaacgtc acttaccagg gacgcaacta ccgctgggga aacacgtcgg
     3841 gcgtctcgcc aaattacgcc gacatcacga atatttccct cagggaaggc cgctttatta
     3901 cggaggctga tgattcgcag cgttcgaatg tgatggtgat tggcgtgaac gcggcggaag
     3961 cgctgtttcc tggttacaac cagatcgcgg gcgcgcaggt gcgcatgggt ggttacaact
     4021 tcgaggtcat cggcgtgctt gaaaagcgca aggcgggttt cttcggcgag aacgaagagg
     4081 acaatgcggt ctatattccg tttcgcaccg cgcaaaagat cgcaccagcg aagggttaca
     4141 tgttgctcgt gattcgtggt cgcacgggca aggttcagga agcgctcgcg cagtcggaag
     4201 agatcctgcg ccgtcggcgt cacgtgaagt ttgacgagcc gaacaatttc gatatcaaga
     4261 cggctgacaa gtttatcgaa cagtttgaca gcatcacggc gatggttgga ctgatcgcga
     4321 tcgcgatatc gagtttggga ttgctcgtgg gcggcattgg cgtgatgaac atcatgctcg
     4381 tgagcgtcac ggagcgaacg cgcgagatcg gtgtgcgcaa ggcgatcggc gcgcggcgtc
     4441 gcgacatcgt gcggcagttt ctgtttgagg cgatgatgct gacgtttctt ggtggtgtgc
     4501 tcggcgt
//
LOCUS       DASSFQ010000053         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_471850, whole genome shotgun sequence.
ACCESSION   DASSFQ010000053 DASSFQ010000000
VERSION     DASSFQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471850"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..2039)
                     /gene="glmS"
                     /locus_tag="VFH96_01650"
     CDS             complement(177..2039)
                     /gene="glmS"
                     /locus_tag="VFH96_01650"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET6972695.1"
                     /translation="MCGIVGYVGNKQVVPLIIDGLRKLEYRGYDSAGIAVVNDEHDLE
                     IRRAEGKLRNLEETIRLSPIDGTYGIGHTRWATHGRPTEENAHPHRDCTGRVVVVHNG
                     IIENYLQLKERLRKTDHHFVTETDTEIMAHLIEEYLKHDASFEQAVRSAVAELRGIFA
                     LAIINADEPDTIIAVRQGPPVVIGLGDREFFVASDIPPILQHTRDVFFLGDGEIAVIN
                     RDAVRVTDFEGNSVQPSIQRITWDPIMAEKGGFKHFMLKEIYEQPRSVRDTVQGRISL
                     DSGQVFLDEMANITEADFKRFTSIKIAACGTSWHAGLAGKYMIEQLARIPVDVDYASE
                     FRYRNPVMDDNSLLLVISQSGETADTIAALREAKELGARVLAICNVQGSMIVREADGT
                     ILTHAGPEIGVASTKAFTGQMIALYLLGLYLGQLRGVLSVEEARFHAQELAELPVKLE
                     HLLNDSDGIEELSKEFFRSTDFLYLGRGINFPVALEGALKLKEISYIHAEGYPAGEMK
                     HGPNALIDERLPVLFVNTREEGNRASELRYEKTHSNIVEVKAREGIVISVLTEGDSIS
                     SMVSDHVIEIPAASDLLSPILSIIPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(2076..3464)
                     /gene="glmU"
                     /locus_tag="VFH96_01655"
     CDS             complement(2076..3464)
                     /gene="glmU"
                     /locus_tag="VFH96_01655"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET6972696.1"
                     /translation="MSNHTASEKPLNVLVMAAGLGTRMKSNRAKVLHELGGSPLIAHV
                     VRAAQKLQPQAILVVVGHQAEEVERAALAEVGDLARFVTQAQQRGTGDAVESARGELE
                     NTDSLVLVLSGDVPMIKTETLRKLIDHHKEAGAACTILSVRLENPTGYGRIVRDANDS
                     FQKIVEHRDATDDQRQVKEINSGIYCFDAKELYRALSKVEPANDQGEYYLTDVAEIIL
                     ADGRTVSVYLHGDNREVSGINTRAELAEFENLIRRAAIRKLMIEDGVTFIDPSHAYIS
                     AEAQIGRDTIIYPNVTVEGKTVIGAGCVIRSGARITNSRLGDNVVVKDHSIVVDSQVD
                     SNCAVGPFAHLRMNATLEEKATVGNFVEVKKSRLKRGTKAMHLTYLGDATIGERTNIG
                     AGTVTCNYDGKNKHETIIEDDVKIGSDTMLVAPVRVGARSMTGAGSVVTKDVPPDSLV
                     TGVPATVKKTLK"
     gene            complement(3477..4523)
                     /locus_tag="VFH96_01660"
     CDS             complement(3477..4523)
                     /locus_tag="VFH96_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET6972697.1"
                     /translation="MYHSSRGVHLQQTTTTGGQKLVAMGGGTGLSTLLAGLKRLVRKA
                     EEEMWIQSLSAIVTVSDDGGSSGRLREELQMLPPGDIRNCMIALSEDSTLLSRLFRYR
                     FRGDGELGGHSFGNLFLAALTEVTGDFTAAIKLSSDILASKGHIFPATISDVRLAAEL
                     EDGTIVRGETSISASKLPIRRLRLEPEQCLPLPEALKAIRAADIITVGPGSLYTSILP
                     NLLVAQVARVIGQSRATKIFVNNLMTQPGETDGYSARKHLDTIKQYAPEIHFDFVVVN
                     NRRISQEQAELYAAEGAFQIGIDDPIDDALDQATEIVRADLLYDGEKVRHNSDRLADV
                     VLNCRKQALAIVPV"
     gene            complement(4635..5480)
                     /locus_tag="VFH96_01665"
     CDS             complement(4635..5480)
                     /locus_tag="VFH96_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6972698.1"
                     /translation="MSSSLTKGLRRSTSRSKAKPAKSAKRAVKAKSPSKPKAKRATAA
                     KTSARKAARPAAAKKTAAKSRPAIKSKKAAPKTVTRKAAPKKVTAVSRVKTTQKQAAV
                     RAIRPAPTAPPAPRPPTVDESAALIAFERAHKEFARGRFAEARNQFRILVDKYSQVSE
                     VAARARTYLAIAESRLRTESILPRDADSLYDRGVIELNRGEYVAAQEMFERALKREPE
                     AAHIHYGLAATRARLGSIDLALESLEKALNLQPNLRVRAQHDHDLNPLRNDPDFDRLI
                     FSNPS"
BASE COUNT         1119 a         1499 c         1565 g         1324 t
ORIGIN      
        1 ggccacggag ggccgcccct acagtacgta ctacgtgacc ctttttgtgt cttgattgcg
       61 cgtataggga aaggcggggg gcagacggct cgtagccaac cgcactgtag ggggatgcag
      121 tgccacacca gccgtttgcc cctgagggga catccgtgtt tgtcggtggt tgatacctac
      181 tcgacggtca cggatttggc caagttgcgc ggttggtcga cgtcacaacc gcgccgcact
      241 gcgatgtgat atgccaggag ttgcagcgga atgatcgaga gaatcggcga gagtagatcg
      301 ctcgcagcgg gaatctcgat cacgtgatct gaaaccattg aagagatcga atcgccttcg
      361 gtcagcacgg aaattacaat accctcacgc gccttaactt ccacgatatt cgaatgcgtc
      421 ttttcataac gcaactctga agcgcgattg ccttcttcac gtgtgttgac aaagagcact
      481 ggcaagcgtt cgtcgatcag cgcgtttgga ccatgcttca tctcgcccgc cggataacct
      541 tcggcgtgaa tgtacgaaat ctctttcaac ttcaacgcgc cttcgagcgc caccggaaaa
      601 ttgatgccgc gaccgagata cagaaaatcg gtcgagcgga agaattcctt cgagagttcc
      661 tcgatgccgt ctgagtcgtt gagcaggtgt tcgagtttca ccggaagttc cgcgagttcc
      721 tgtgcatgga agcgagcctc ttcgaccgaa agcacgccgc gtagttggcc cagatacaac
      781 ccaagcaggt agagcgcaat catctgccct gtgaaagctt tggttgaagc gacaccgatc
      841 tccggcccag cgtgcgtaag gatcgtgccg tccgcttcgc gaacgatcat cgaaccctga
      901 acgttgcaga tcgcgagaac tcgtgcgccg agctctttcg cttcacgaag tgctgcgatc
      961 gtgtcggcag tttcgccgga ctgagaaatc accagcaaca acgagttgtc gtccatcacc
     1021 ggattgcgat agcgaaactc ggaagcgtaa tcgacgtcga ccggtatgcg tgcgagttgc
     1081 tcgatcatgt acttgcccgc aagacccgca tgccacgacg tgccacacgc cgcaatctta
     1141 attgacgtga accgcttgaa gtccgcttcg gtaatattgg ccatttcgtc gagaaatacc
     1201 tggccgctgt cgagcgagat tcgtccctga accgtgtcgc ggactgagcg cggctgctcg
     1261 taaatctctt taagcatgaa atgcttgaag ccgccttttt ccgccatgat cggatcccac
     1321 gtgatgcgct gaatcgatgg ctgaacggaa tttccttcga agtctgtgac ccgcacggcg
     1381 tcgcggttga tgaccgcgat ctcaccgtcg ccgagaaaga agacgtcgcg cgtgtgttgc
     1441 agaatcggcg gaatatcgga cgcgacaaaa aattcgcggt ctcccaaacc gataacgacg
     1501 ggcggtccct gacgcacagc gatgatcgtg tcgggctcgt cggcgtttat gatcgcgagc
     1561 gcgaagatac cgcgcagttc agccacagca gaacgcacgg cctgctcgaa cgaggcatcg
     1621 tgtttgagat actcctcgat cagatgggcc atgatctcgg tgtcagtttc ggtgacgaag
     1681 tgatgatccg ttttgcgcag tcgctctttt agttggagat agttctcgat gatgccgttg
     1741 tgtacgacga cgacgcggcc ggtgcagtcg cggtgcggat gtgcgttttc ttcagtggga
     1801 cggccgtgtg tggcccaacg cgtgtggccg atgccgtagg tgccgtcgat aggactgaga
     1861 cggatcgtct cttcgaggtt gcgcagtttt ccttcagcgc ggcggatctc gaggtcgtgc
     1921 tcgtcgttga cgaccgcgat gccggctgag tcgtaaccgc gatattcgag tttacgaagg
     1981 ccgtcgatga tgagcggcac tacttgcttg tttccaacat acccgacgat tccacacatc
     2041 ctttatccaa ctcctctatc cacagattac gcagattact tcaaggtctt ttttactgtt
     2101 gcagggacgc ccgtgactaa cgaatccggt gggacgtctt tggttacaac tgatccggcg
     2161 cctgtcatcg aacgagcgcc gacgcgaacg ggtgcgacca gcatcgtgtc ggagccgatt
     2221 tttacgtcgt cttcgatgat tgtttcgtgt ttgtttttgc cgtcgtagtt acaagtcacg
     2281 gtgccggcgc cgatgtttgt gcgctcgcca atcgttgcgt ctccaaggta tgtgagatgc
     2341 atcgccttgg tgccgcgttt cagacgcgat tttttgacct cgacaaaatt tccgaccgtc
     2401 gctttctctt cgagtgtcgc gttcatacgc aggtgtgcaa acggtcccac cgcgcagttt
     2461 gaatctacct gcgaatcgac gacgatcgag tgatccttca cgactacgtt gtcacccaaa
     2521 cgcgagtttg tgatccgcgc gccggaacga atcacacagc cggcgccgat cacggtcttg
     2581 ccttccaccg tgacgttcgg ataaatgatc gtgtcgcgcc cgatctgtgc ttcggcgctg
     2641 atgtatgcgt gcgatggatc gatgaaagtc acgccgtctt caatcatcag tttgcgaatt
     2701 gccgcgcggc ggatgaggtt ttcaaactcg gcgagctcag cgcgggtgtt gattccggaa
     2761 acctcgcgat tgtcgccgtg gagataaaca ctgactgttc ggccgtcagc aaggatgatc
     2821 tcggcgacat cggtcagata gtactcaccc tgatcgttag ccggctccac tttgcttagg
     2881 gctcgataca gctctttcgc gtcgaaacaa taaatgcccg agttgatttc cttgacctgg
     2941 cgttgatcgt ccgtggcatc tctgtgctcg acgatcttct gaaacgaatc gtttgcatca
     3001 cgaacaatgc ggccgtagcc ggtcgggttt tccagacgaa cactcaggat cgtgcaggcc
     3061 gcgccagcct ctttgtgatg gtcaatcagt ttacggaggg tttcagtctt gatcatcggc
     3121 acgtcgcctg aaagaaccag cacgagtgag tcagtgtttt cgagttcacc gcgcgcagat
     3181 tcgacagcat caccagttcc tcgttgttgt gcttgggtca caaaccgagc gagatcgccg
     3241 acttccgcca gtgccgcacg ctcgacctct tccgcctgat gacccaccac caccagaatt
     3301 gcttgcggtt gcaacttctg agcggctctg acgacgtgcg cgatcaacgg cgagccgccg
     3361 agctcgtgca ggaccttggc gcggttcgac ttcattctcg taccaaggcc tgccgccata
     3421 acgagtacgt tgagtggttt ttccgaggcg gtgtggttgg acatcgataa gttatctcag
     3481 acgggaacaa tggcgagtgc ttgctttctg caattcagca cgacatcggc aagacgatcg
     3541 gagttatgcc ggactttttc accgtcgtac aacaagtcag cccgcacaat ttcggtagcc
     3601 tgatcgagcg cgtcgtcaat cggatcgtca atcccgatct gaaatgcacc ctcagccgca
     3661 taaagttctg cctgctcctg gctgatgcgc cggttgttga cgaccacaaa atcaaaatga
     3721 atctcaggcg cgtactgttt gatcgtatcg agatgtttgc gcgcgctgta gccgtctgtt
     3781 tcgcccggct gagtcatcag gttgttgacg aagatttttg tcgcgcgtga ttgaccgatt
     3841 acgcgtgcga cctgggcgac aagcagattg ggcaggatgc tggtgtagag tgaacccgga
     3901 cccacggtga tgatgtcagc ggcacgaatg gcttttagtg cttcaggcaa gggaagacat
     3961 tgctctggtt cgagccggag gcgacgaatc ggaagtttcg aagccgagat cgaggtttca
     4021 ccgcgcacga ttgtgccatc ttccaactcc gctgccaggc ggacgtcgct gatcgtcgcc
     4081 gggaagatgt gacccttgct cgccagaatg tcagacgaca attttatcgc ggcggtaaag
     4141 tctccggtta cttcggtcaa ggctgccaga aagaggtttc caaaactgtg tccacccaac
     4201 tcaccgtcgc cgcgaaagcg ataacggaag agacgcgaca agagagtcga gtcctccgag
     4261 agtgcgatca tgcaattgcg aatatccccg ggcggcaaca tctgcaactc ttcgcgcagg
     4321 cgtccggaac tgccgccgtc atcagaaaca gtcacgattg ccgaaaggct ttgaatccac
     4381 atttcctctt cggcttttcg aaccaaacgc ttgagcccgg cgaggagcgt cgagagcccg
     4441 gtgccgccac ccatggctac cagcttttga ccgcctgtag ttgttgtttg ttgcaaatga
     4501 acgcctcggc tcgaatgata cattaatctt gtgatttgtt tctcgcaaag gcgctaagac
     4561 agcaaagtaa actattcaat ctagaaattc ttctttgcgc cttaggagaa ctttgcgtct
     4621 ttgcgggaaa ctgcttaaga ggggttgcta aagatcagcc ggtcgaaatc ggggtcgttg
     4681 cgtagtggat tcaggtcgtg atcgtgttgc gctcgcacac gaagattggg ctggaggttc
     4741 aaagctttct cgagagactc cagcgccagg tcgatggaac cgagtcgagc gcgggtagca
     4801 gcaaggccat aatgaatgtg cgctgcttcg ggctcgcgtt tgagagctct ctcaaacatc
     4861 tcttgcgccg cgacatattc gcctcgattg agttcgatga cgccgcggtc atagagtgag
     4921 tcagcatcgc gtgggagaat actttcagtc cgcagacgcg actccgcgat cgcgagatac
     4981 gtgcgagcgc gagctgcgac ttccgaaacc tgcgagtact tatcgacgag aattcgaaac
     5041 tggttgcgcg cctctgcaaa ccggcctcgg gcaaattcct tatgtgcgcg ttcgaatgca
     5101 atgagtgccg ccgactcgtc taccgtcggt ggtcgtggcg ccggcggagc tgttggcgct
     5161 ggtctgatgg cgcgaacagc agcttgcttc tgcgtcgttt tgacccttga tactgcagta
     5221 actttcttcg gagcagcttt acgagtgact gtcttcggtg cagccttctt cgactttatt
     5281 gcgggccgcg acttggcggc ggtctttttg gccgcggctg gtctcgccgc ctttctcgca
     5341 gatgttttag cggcggtggc tcttttggct ttgggtttgg agggcgactt cgctttaaca
     5401 gcgcgtttcg cgcttttcgc tggttttgcc tttgaacgcg atgttgaccg tctcaatcct
     5461 tttgtgagac tgctgctcat gtgcggatct caaaaggctg ctcgctt
//
LOCUS       DASSFQ010000054         8297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_919448, whole genome shotgun sequence.
ACCESSION   DASSFQ010000054 DASSFQ010000000
VERSION     DASSFQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919448"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFH96_01670"
     CDS             <1..519
                     /locus_tag="VFH96_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6972699.1"
                     /translation="AITHGIYLIPEDRRLTGLIVDFNVRENISLPNLDHYSSAKIINF
                     AKEANAAREAVKAINIKTPSPEMRAANLSGGNQQKVVLARWLTFSPRVLIFDEPTRGI
                     DVGAKSEIYELIRKLASEGVSVIVISSEMEEVLGISDRIAVMHEGRLTGILDRAQFSE
                     EAVMRLATGTEG"
     gene            570..1547
                     /locus_tag="VFH96_01675"
     CDS             570..1547
                     /locus_tag="VFH96_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972700.1"
                     /translation="MKKELGIFVLLIVLCIIVSIINPNFLLAINLQNLARQIGAFGIF
                     SIGLGLVIITGGIELSVGSMMALLGVLLSMMLTEGNMGVALAVLACIGIAMALTLGHG
                     LLITRLNMQPFIVTLCGLLFYRGLARFITNDETKGFGTVAGLDFLRSLANGNLFGIPR
                     PFVLLLIIAAITWVVLHRSVYGRYLFATGRNAEAARYAGINTKWIVTFTYVIAGALTA
                     ISGVIFAFYTNSVSPANHGNAYELYGIAAAVLGGCSLRGGEGSVVGILIGTALLQVLR
                     NLVNLLGIPSSLDFAVMGAVILIGVMADQIFTERRNRRQIALMRESESH"
     gene            1565..2701
                     /locus_tag="VFH96_01680"
     CDS             1565..2701
                     /locus_tag="VFH96_01680"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HET6972701.1"
                     /translation="MRLHRKISRSLVAVLWLLAIVVVTQAQVTKQSFGKTETGENVDL
                     YTLKNTRGAEATITNFGGIVVSLKVPDRTGKFDDVVLGFSDLDSYLKPGPYFGALIGR
                     YGNRIAKGRFTLNSVEYKLAVNNGENHLHGGIKGFDKVVWTGKEMKTASGPAVVLTYL
                     SKDGEEGYPGNLNVRVVYTLTNNNELKIDYSATTDKDTVTNLTHHSYFNLAGEGNGDI
                     LNTRVTINGSRFVPTDAGSIPLGQLKNVAGTPFNFLTAHAIGERINQDDEQIKFGSGY
                     DHTWVINGRPGVMRLAATAYEPTSGRVMQVWTTEPGMQFYTGNFLDGTLTGKSGKIYP
                     RRSGFCFETQHYPDSPNQPSFPTTTLKKGATYKSTTIYRFSTRR"
     gene            complement(2791..4167)
                     /locus_tag="VFH96_01685"
     CDS             complement(2791..4167)
                     /locus_tag="VFH96_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6972702.1"
                     /translation="MSTPVKPPSFSPLDPEIKRGRLKFERRITILALLAGFPAVALSA
                     LLLWYDGYSAQVQWTVDLLLVLLWLGIAFNLKTRIVRPLQTLSNILAAIREGDYSIRG
                     RRALSGDALGEVMLEVNDLGQTLREQRLGAMEATALLRTVMAEIDVAVFAFDGEQRLQ
                     LVNRAGQRLLAQPSARLLGRTADELGLSACLNSSAGSAPQTMQMVFPGGVGRWDIRRS
                     TFREGGQQHQLLVLTDLSQTLREEERTAWQRLLRVLGHELNNSLAPIKSVAGSLADLM
                     GREPEPPDWRDDMHRGLAVISSRADSLARFVESYSKLARLPPPRLEPMKIGALVSRVA
                     SLETRLHVNVVAGPDVLVRGDDVQLEQLLINLVRNAVDASMETRGGVDVGWQQKNGQV
                     EVWVRDEGPGLASTANLFVPFFTTKAQGSGIGLVLSRQIAEAHGGTLTLENRQQLHGC
                     EARLRLPL"
     gene            complement(4273..5649)
                     /locus_tag="VFH96_01690"
     CDS             complement(4273..5649)
                     /locus_tag="VFH96_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6972703.1"
                     /translation="MPRILIADDQADVLAALGLLLKGERFQIESASSPEGVLKAIDVH
                     ELDVVLIDLNYARDTTSGNEGLDLLSRIRAVDPTLPVVVMTAWGSVDLAVEAMRRGAR
                     DFIQKPWDNARLLAVLRTQVELGEALRRGRRLEAENMALRDSVAEANDLPQVVADSPA
                     MKAVLNLVERIGPADANVLITGENGTGKEVITRLLHSISSRRAKPLISLNAGALAEGV
                     FESELFGHVRGAFTDAKSDRVGRFELADGGTLFLDEIGNVPLNLQAKLLRVLETGEFE
                     RVGSSKTQKVNVRLLSATNADLNAEVAQGRFRQDLLFRLNTVEIRLPPLRDRLDDIPV
                     LAEHFLKLHRERYRRPIMGFTPEALDALRHHLWPGNVRELDHVIERAVLMSPGSVVTA
                     FDLALEATPDARLSARLEEMSLEDAERLLIKKALARFEGNANRAAEALGLSRSALYRR
                     LQKYGLGS"
     gene            complement(5674..8100)
                     /locus_tag="VFH96_01695"
     CDS             complement(5674..8100)
                     /locus_tag="VFH96_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972704.1"
                     /translation="MANLRYAIRTLVKQPGFAAVVVITLALGIGASTAIFSVVNAVLL
                     RPVPLKHSDRLAVVWGNFRALNIQHLPAKTAEYLDYADQKQVFDSVAAYAGHSFNLST
                     GNEPERIRGTIVSGNLFPMLELQPAIGRLFASDAEHEVVLNDSFWQRRFGGDRSIINQ
                     RIELDGESYTVVGVMPAGFQFPHASFSWAEPADVWVPLNVRDVDQAGRRGPYYLNVLA
                     RLAPGVTLEQARTHMNALAQRFERELSGYRGPKGEDGGWHITVTPLQEEIVGSSRRAL
                     LVLLFSVSLLLLIACANVANLLLMRSARRHRELAIRAALGASRWQIARQLIIEGLLLS
                     LFAAALGLVFVNWGIDLLATLGPEIVPRAHEVSIDARVFGFMALAVVIVSVGFGLVAA
                     APVSRLDLQEALRNTRQTGGVHRGPWNNILVVAEVSLAVLLLVGSGLLIKSLLRLQQT
                     NPGIVANQLTSVEIDLSATTYTEPERASDFYRQLVAQVETLPGVQSATFSTRQPLSGS
                     GGSDPFAIEGRQLDPSNLTAAGWQVVGPNYLETLGISIVKGRDFTQQDTEPGATPVAV
                     VNEKLVTRFFPNEDPIGRRLTLGLPRPGNPWITIIGVARNVPHGALDSNAEPDWYTSK
                     VVSPQRHRFLFVRSALPAASLTAAIRKEMASIDPHQPFTSVKTMEEVISTTTAPRRFN
                     ALLLGVFAAVALLLAALGIYSVISYSIAVRTQEIGIRMALGASRRGILLLVMRKGLLL
                     TVIGTLIGLAGAFALTRWMSSLLFGVTASDPATYVFVLLVALGAAVLACLIPARRATR
                     VDPLVALRYE"
BASE COUNT         1953 a         2435 c         2220 g         1689 t
ORIGIN      
        1 gccatcacgc atggtattta cctcatcccg gaagaccgcc ggctcactgg actgatcgta
       61 gatttcaacg tgcgtgaaaa catctctctg ccaaacctgg atcactactc gtcggcaaag
      121 atcatcaatt tcgccaaaga agcgaatgcg gcacgcgagg cggtcaaggc gatcaatatc
      181 aaaacgccct cgccggagat gcgtgctgca aatctcagtg gcggcaatca gcaaaaagtg
      241 gtgctggcga ggtggctgac gttctcgcca cgagttctga tttttgacga gccgacacgc
      301 ggcatcgacg tgggcgcgaa gtcggagatt tatgaactca tccgcaagct ggcaagcgaa
      361 ggtgtctcag taatcgtcat cagcagtgaa atggaagagg tgctcggaat cagcgatcgc
      421 atcgccgtca tgcacgaagg acgactaacc ggaattctcg atcgcgctca gttcagcgag
      481 gaagccgtca tgcgcctcgc caccggcacc gaaggatagc cacaaaaagg cccaaaagtt
      541 gtgctttttg tggctaaacg aggctaatta tgaaaaaaga gcttggcatt tttgtactgc
      601 taatcgttct ctgcattatc gtctcgatca tcaacccaaa ttttctgctc gcgattaatc
      661 ttcagaacct cgcgcggcag atcggcgcct tcggcatctt cagcatcggg ttgggtctag
      721 tgatcatcac cggcggcatt gaactctcgg tgggttcgat gatggcgcta ctcggcgtcc
      781 ttttgtcgat gatgctgacc gaaggaaaca tgggcgttgc tttagcggtg ctggcatgta
      841 tcggaatcgc gatggcgctg acgttgggcc acggcttgct catcactcgg cttaacatgc
      901 aaccgttcat cgtgacgctg tgtggcctgc ttttctatcg cggtctcgca cgtttcatca
      961 ctaacgatga aacaaaagga ttcggaacgg tggcgggact cgacttcctg cgctctctcg
     1021 cgaacggaaa tctattcggc attcccaggc cgtttgtctt actgctaatc attgctgcga
     1081 ttacatgggt cgtgttgcat cgttcggtct acggccgtta tctgtttgcg accggccgta
     1141 atgccgaggc agcacgctac gcgggcatca acaccaaatg gatcgtcacc ttcacctacg
     1201 taatcgccgg agcgttgact gcaatctcag gcgtgatctt cgccttctac accaactcag
     1261 tctcgccggc gaatcacggc aacgcgtatg aactctatgg aatcgccgcc gcagtactcg
     1321 gtggctgttc gttgcgaggc ggtgagggct cggtcgtagg catcttgatc ggcactgccc
     1381 tgcttcaggt gctgcgcaat ctcgtcaacc tgctcggcat tcccagttca ctcgattttg
     1441 cagttatggg tgcggtgatc ttgatcggcg tgatggcgga tcagatcttc accgagcgcc
     1501 ggaatcgtcg acaaatcgcg ttgatgcgcg agagcgagtc ccattaatgg aaatggagaa
     1561 acgaatgaga ctacaccgca agatttcgag aagtctggtt gccgtattgt ggttgttggc
     1621 gatcgtcgtc gtcactcagg cgcaggttac caagcaaagc tttggtaaaa ctgaaaccgg
     1681 cgagaacgtc gatctctaca cactcaagaa cactcgcggc gccgaagcca cgatcaccaa
     1741 cttcggcggc attgtagtct cactgaaagt tcctgatcgc accggaaaat ttgacgacgt
     1801 cgtgctcggg ttcagcgatc tcgacagcta tctgaaaccc ggtccgtact tcggcgcact
     1861 cattgggcgc tatggcaatc gcatcgctaa aggacgtttc actttgaaca gtgtcgagta
     1921 caagctcgca gtcaacaacg gcgagaacca tcttcacggc ggcatcaaag gcttcgacaa
     1981 agtcgtctgg accggtaagg aaatgaagac ggcatccggg cccgcggtcg tgctgactta
     2041 tctgagcaaa gatggcgaag agggttatcc tggaaatctc aacgtcagag tggtctacac
     2101 cctaaccaac aacaacgagc tcaagatcga ctattcagcg acaaccgata aagacaccgt
     2161 cacaaacctg acgcatcact cgtacttcaa tctcgcgggt gaaggcaatg gcgacattct
     2221 caacacgcgc gtcacgatca acggcagccg gtttgtgccg actgatgccg gttcgatacc
     2281 cctcggtcaa ttgaaaaatg ttgccgggac gccgttcaac tttctcaccg cgcacgcgat
     2341 tggcgagcgt atcaaccagg atgacgagca aatcaagttc ggtagcggat acgatcacac
     2401 gtgggtgatc aatggccgtc cgggtgtgat gcgacttgcg gcaactgctt atgaaccgac
     2461 gagcggtcgc gtaatgcagg tgtggaccac cgaaccgggt atgcagtttt acacgggaaa
     2521 ctttctcgac ggcacattga ctggtaagtc cgggaagatc tatccgcggc gatcaggttt
     2581 ttgcttcgag acgcaacatt atcccgactc tccgaaccaa ccctcgtttc cgacaacgac
     2641 gttgaagaag ggtgcgactt acaaatcgac gacgatctat cggttcagca ccagacgtta
     2701 gacaggataa acaagatgtt ttcaggattt acaagatgaa tcctgtaaat cctgaaacaa
     2761 atcttgttca tcctgtccaa ccagacccca tcataaaggc aatcttagtc gcgcttcaca
     2821 cccgtggagc tgttgccggt tttcgagcgt caacgtccca ccatgcgcct ccgcaatctg
     2881 ccgcgataac accaacccaa ttccggatcc ctgcgctttc gtcgtaaaaa acggcacgaa
     2941 gagattcgcc gtactcgcga gtcccggtcc ttcatcacgc acccacactt ccacctgacc
     3001 gttcttctgc tgccaaccaa cgtccacgcc gccgcgcgtc tccatcgatg catccaccgc
     3061 attgcgaacc aggttgatca gcaactgctc gagttgcaca tcatctccgc ggaccagcac
     3121 atccggacca gcaacaacat tcacgtgcag ccgcgtctcc aaactcgcca cccgcgacac
     3181 gagcgcgccg attttcatcg gctcaagccg cggcggcggc aggcgcgcca gttttgaata
     3241 cgactcgacg aaacgtgcga gcgaatcagc acgcgacgaa atcaccgcca gaccacgatg
     3301 catatcgtcc cgccaatcgg gcggttccgg ttcccttccc attaagtctg caagacttcc
     3361 agccaccgac ttgatcggcg cgagcgagtt gttgagttcg tggcccaaca cgcgtaacag
     3421 acgctgccag gccgtgcgtt cctcttcacg aagcgtttgg ctcaagtcag ttagcaccag
     3481 caactgatgt tgttgtccgc cttcacgaaa cgtgctgcga cgaatgtccc agcggccaac
     3541 gcctcccgga aaaaccatct gcatcgtttg cggcgcagaa cccgcggatg agttgagaca
     3601 cgctgaaaga cccagttcgt cagcggtgcg accaagcaaa cgtgccgagg gttgggccaa
     3661 caatctttgt cctgcgcgat tcacgagttg caggcgctgt tcgccgtcga aagcaaagac
     3721 ggcgacgtca atctccgcca tgaccgtgcg cagcagcgcc gtggcttcca tcgcgccgag
     3781 acgttgctca cgcagtgtct ggcccaggtc gtttacttcg agcatcactt cgccgagcgc
     3841 gtcacccgaa agcgcacgcc ggccgcgaat ggagtagtcc ccctcgcgaa tcgcagccag
     3901 gatgtttgag agtgtttgca gtggacggac aatgcgagtt ttgagattga aagcgatgcc
     3961 gagccaaagc agcaccagca acaagtcgac ggtccactgg acctgagctg aatagccgtc
     4021 gtaccaaagc aacaatgcag acagagccac tgcaggaaaa ccggcgagga gcgcgaggat
     4081 cgtgatccgt ctttcaaatt tcagacgccc gcgtttgatt tccggatcga gcggagaaaa
     4141 cgatggcggc ttaacgggcg tactcacagc caccataata acagccacaa aaaggcacaa
     4201 gaagcacaaa actgattaat gaacgcctcg gctcagtaag aatctttttg tgctttttgt
     4261 gcctttttgt ggctaagatc cgaggccata tttctgtagg cgacgataga gtgcgctgcg
     4321 actgaggccg agggcttcgg cggcgcggtt ggcgttgcct tcgaaacgag cgagggcttt
     4381 cttgatcagg agacgttctg catcttcgag gctcatctct tcgagtcgtg ctgacaggcg
     4441 cgcgtcaggc gtggcttcga gtgcgagatc gaatgcggtc acgacgcttc ccggactcat
     4501 caacacagct cgttcgatga cgtggtccag ttcgcgcacg ttgccgggcc agagatgatg
     4561 acgcaacgca tcgagcgcct ccggcgtgaa acccatgatc ggacgccggt aacgctcgcg
     4621 atgcagcttc agaaaatgct cggcgagcac aggaatatcg tcgagccgat cgcgaagtgg
     4681 cggcaaccga atctcgactg tgttgaggcg gaaaagcaaa tcctgacgaa atcgtccctg
     4741 agccacttcg gcatttagat cagcattcgt agccgacaac aagcgcacat tgaccttctg
     4801 agtcttcgaa gatccgacgc gttcaaactc acctgtctcg aggacgcgca acagctttgc
     4861 ctgtagattc aatggcacat tgccaatctc gtcaaggaag agcgtgccac catcggcaag
     4921 ctcgaaacgc ccgacacgat cgctcttcgc gtcagtgaaa gcaccgcgca catgcccaaa
     4981 caactcgctt tcaaaaaccc cttcagccag cgccccggcg tttaatgaaa tcagcggctt
     5041 ggcgcgtcgc gacgagatcg aatgaagcaa acgcgtgatc acttctttgc cagtgccatt
     5101 ctcaccggtg atcaaaacgt tcgcatccgc cggaccgata cgctcgacca gattcaatac
     5161 cgccttcatc gccggcgaat cggccaccac ctgtggcaag tcgttcgctt ccgccacact
     5221 gtcgcgcaac gccatgttct cggcttccaa acggcgtccg cgcctgagtg cctcgccgag
     5281 ctcaacctgc gtgcgtaaca ctgcgagcag gcgcgcgttg tcccatggct tttggataaa
     5341 gtcgcgggcg ccgcgtcgca ttgcttcaac ggcgagatcg acgctgcccc aagcagtcat
     5401 gaccaccacc ggcaacgtcg gatccaccgc gcgaatgcgc gaaagcaagt ccaaaccttc
     5461 attgcccgac gtcgtatcgc gagcgtagtt caggtcgatc aaaaccacgt cgagctcatg
     5521 cacgtcgatc gctttgagca caccttcggg tgacgacgcg ctttcgatct gaaaacgctc
     5581 acccttcaat agaagaccca acgccgcaag gacgtcggcc tgatcatcag caattagaat
     5641 gcgaggcata gactggagat aatgccatgg atgtcattca tatcgtagcg ccacgagcgg
     5701 atcgacacgc gtagcccgtc gcgccggaat caaacacgcg agcactgcgg cgccgagcgc
     5761 taccaacaac acaaacacat aagtcgcggg atcagaggcc gtgacaccaa acaagagact
     5821 cgacatccag cgcgtcagag caaatgctcc tgccaatccg atcaaggtgc cgatcaccgt
     5881 aagcaacaat cctttgcgca tcactagcaa caaaatccca cgtctgctcg cgccgagcgc
     5941 catgcgaatg ccgatttcct gtgtccgcac ggcaatcgaa tacgagatca cgctgtaaat
     6001 cccaagcgcc gccagcaaga gcgcgaccgc ggcaaacacg ccgagcagca acgcgttgaa
     6061 acgacgaggc gcagtggtgg tgctgatcac ttcctccatc gttttcacgc tggtgaatgg
     6121 ttgatgcgga tcgatgctcg ccatttcttt ccgaatcgcc gcggttaaag acgccgccgg
     6181 caacgcactg cgtacaaaga gaaaacgatg tcgctgcgga gagactactt tcgacgtgta
     6241 ccagtcgggc tcagcattcg aatcaagcgc gccgtgcgga acgttgcgcg ccacgccgat
     6301 aatcgtgatc cacggattgc ccggacgcgg cagaccgagg gtgaggcgac gtccaatcgg
     6361 atcctcgttc gggaaaaagc gcgttactaa tttttcatta acgacggcaa cgggagttgc
     6421 gccaggttcg gtatcctgtt gggtgaaatc gcggcctttg acgatggaga taccaagcgt
     6481 ctcgaggtaa ttcggaccga caacctgcca gccggccgca gtcaagttcg atggatcgag
     6541 ttgccggcct tcgattgcaa acggatcact gccgccgctt ccgctcaaag gctgtcgcgt
     6601 actgaacgtc gccgattgca cgccgggcag cgtctcaacc tgggccacca gttgccggta
     6661 aaaatccgac gcgcgctcgg gctcggtata agttgttgca gacagatcga tttcaacact
     6721 cgttagctga tttgcaacga tgccgggatt cgtctgttgc aaacgcaata gactcttaat
     6781 cagtaatcct gatccaacca gcaacaacac ggcgagtgaa acttcagcca ccacgaggat
     6841 gttgttccat ggtccgcggt gcacgccgcc cgtctggcga gtgttccgca acgcttcctg
     6901 caagtcgagt ctcgaaaccg gtgcagcggc aactaaacca aaaccgacac tgacgatgac
     6961 caccgcgagc gccatgaagc caaacacacg cgcgtcaata ctgacttcgt gtgcacgcgg
     7021 cacaatctct ggtccaagtg tcgcgaggag atcgatgccc cagttcacaa acaccagccc
     7081 gagagctgcc gcaaacaatg agagcaacaa tccttcgatg attaactgtc gcgcgatctg
     7141 ccaacgactg gccccgagcg ccgcgcgaat cgcgagttcg cgatgacgtc tcgccgaacg
     7201 catcaacaac agattggcga cattcgcaca cgcgatcagc agtaacaaac tcactgaaaa
     7261 caacaacacc agcaacgcgc gtctgctgct gccgacgatt tcctcctgca aaggcgtgac
     7321 ggtaatgtgc cacccaccgt cttcgccctt tgggccacga taaccggaca actcacgctc
     7381 aaaacgttgc gcgagcgcat tcatgtgcgt gcgcgcctgt tcgagtgtga cgccgggtgc
     7441 aagccgcgcc aacacgttca aatagtacgg tccgcggcgg ccagcctgat ccacatcgcg
     7501 aacattcaac ggcacccaaa cgtcggccgg ctcagcccac gaaaaactcg cgtgtggaaa
     7561 ttgaaacccg gccggcatca ctcccaccac cgtgtaactc tcgccgtcga gctcgatccg
     7621 ttgattgatg atcgagcggt cgccgccaaa gcgtcgttgc cagaacgagt cgttcaaaac
     7681 cacttcgtgc tcggcatcac tcgcgaacag tcgcccgatc gccggttgca gttcgagcat
     7741 cggaaacaga ttgcctgaca cgatcgtgcc gcgaatgcgt tccggctcgt ttccggttga
     7801 taagttgaag ctgtgcccag catacgcagc gactgaatca aatacctgtt tctgatcggc
     7861 gtaatcgaga tactccgcgg tcttcgcagg gaggtgttga atgttcagcg cgcgaaagtt
     7921 tccccaaacc actgctaagc gatcggagtg cttgagcgga actggccgca gcaaaacggc
     7981 gttgacgacg ctgaagattg cagtggacgc gccgatgccg agggcgagcg tgatgacaac
     8041 cacggctgcg aagcccggtt gctttactag tgttcggatt gcgtatcgta ggttggccac
     8101 ggattttacg gatctctctt taaagatcga tttgcacgga ttttattcgt atctaagagc
     8161 ttcgattggg ttgaccttcg ttgctctgcg tgcgggtaca aagcaggcga ttagagcgac
     8221 gagggccagc aatagcgtca cgctcgcgaa tgtcagcgga tcagttgcgc tgaccggcaa
     8281 tggcaactca agttcta
//
LOCUS       DASSFQ010000055         4724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1512426, whole genome shotgun sequence.
ACCESSION   DASSFQ010000055 DASSFQ010000000
VERSION     DASSFQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512426"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFH96_01700"
     CDS             <1..949
                     /locus_tag="VFH96_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972705.1"
                     /translation="VYDASKTAVYDNKSFRIFQDAQCRVEVVLGEFYWKVETGEQVRG
                     VDYVKPPLMLSKEVSTVYVSDPNAKKKKRTTGEINWSLGTYMPVQEIEKRFSVTGLPR
                     PSNIAPNQPYKHWWIYKYWLAFIVLLGLAGVLAFVFAGSTSQAYSQTVNFPALPNEDG
                     TQVFFSEPFELKGRRNIRIVGQSPVQNTWVYLEGDLINDETGVVQSFPIDISYYQGVE
                     DGESWSEGGQSDSAFTSSLPAGRYVLRLEGQWEKWSQPAVVSVQVEQNVTRGFNFLIA
                     LIVLSIGPVLMIVYHIGFERRRWSESMFGGGDDSDDDDD"
     gene            1038..1214
                     /locus_tag="VFH96_01705"
     CDS             1038..1214
                     /locus_tag="VFH96_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972706.1"
                     /translation="MLKALYTIFAVGVVSLYSAAALLGWEISGGSRRQLPPDARQNGY
                     RSFHFWHYGLHGGK"
     gene            1214..1459
                     /locus_tag="VFH96_01710"
     CDS             1214..1459
                     /locus_tag="VFH96_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HET6972707.1"
                     /translation="MLDATSFGLFIPMENLLPVVVTTLVFVVIGLIVFALAFLVIAKA
                     APFSVRKEIEDDQNIALAIVIAAVILGSALIIAAAVH"
     gene            1485..2990
                     /locus_tag="VFH96_01715"
     CDS             1485..2990
                     /locus_tag="VFH96_01715"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET6972708.1"
                     /translation="MKRPPLLFLNVLIVATCGLIYELLAGTLASYVLGDSVTQFSLII
                     GIYLFAMGVGSWLSRFIDKGLARRFVDIELAVAVLGGFSAPILFLTFSRVSYFYVVLY
                     FIVFAIGVFVGLEIPLLLRILKDEIEFKELVSRVLAFDYVGALVASILFPILFVPRLG
                     LIRTSLIFGMLNAGVALWGTWMLRPLIKGRITGLRVRAIVVMVLLVIALVKANRITSL
                     AEDEMFADEIVYTKDTPYQRIVITRGRAGFQLFLNGNLQFSSTDEYRYHEALVHPAML
                     LSNNPRRVLVLGGGDGLALREILKYPSVEHITLVDLDPEMTKLSSAFPLLAKLNQNSF
                     NDPRVHVINEDAFIWLEKTNEPLYDAAIVDFPDPNSFALGKLYTTRFYRLLKSRLTEN
                     AGVSVQSTSPMFARNSYWCIIRTIEAAGFVVRPYYTAVPSFGLWGYALARSSSFEVPQ
                     NPPPGLKFLDEQTLAAMFTLSKDIEPVPVEINRLDNQVLVRYYEGEWRKFE"
     gene            3099..4706
                     /locus_tag="VFH96_01720"
     CDS             3099..4706
                     /locus_tag="VFH96_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET6972709.1"
                     /translation="MNRREILAAFLGVPFALAACRRNEELPLPDGEIVGASDVFGHRL
                     RDGLHVEIPQDAWSNVPLVIVGGGVAGLTAAWRLRNNGFTNFTLIELESAPGGTSRSG
                     SNRLISFPWGAHYIPAPLKENAALVSLLDEMGVVEGKDKDGEPIIGEQFLTRDPEERV
                     FYKGRWYEGLYLHAGESADDQQQFQKFNAEVDKWIAWRDGKGRRAFTLPVSACSDDAE
                     VTLLDRVSMSEWMGARGLTSARLRWWVDYACRDDYGMTLDHTSAWAGLFYFCSRAPAP
                     KTESQPLITWPEGNGRLVQHLFEKAKDNIQLDRAAVELIPTDNGVDVITIDHNGREPR
                     GFHAQRVIFAAPQFVARYVVRPYRDNPPPHIADFQFGAWMVANLTLKDRPKPGSQRDF
                     PLAWDNVFYESPSLGYVVATHQRGLDRGPTVLTYYYPLVDENPRVGRTRLLETDWRGW
                     AEVTLSDLSRAHQDIRGLVERLDVMRWGHAMIRPRTGFMWGAARREGAKPFRGIHFAH
                     SELSGVALFEEAFDNGLRVGNEILSIL"
BASE COUNT          973 a         1223 c         1379 g         1149 t
ORIGIN      
        1 cgtatacgac gcgtcgaaga ccgcggtgta tgacaataag agttttcgaa tttttcagga
       61 cgcacagtgc cgcgtcgagg tcgtgctcgg tgagttttac tggaaggtcg aaaccggcga
      121 gcaggtgcgc ggcgtcgatt acgtcaagcc gccgttgatg ctttcgaaag aagtttcgac
      181 tgtttacgtc agcgatccaa acgcgaagaa gaaaaagcgc accacgggtg agatcaactg
      241 gtcgctcggc acgtacatgc cggttcagga gatcgagaaa cggttctcgg ttacgggatt
      301 gccgcggccg tcgaacatcg cgcccaatca accttacaag cactggtgga tttacaaata
      361 ctggctcgcg tttattgtgc tactggggct ggcgggcgtt ctggcgttcg tctttgcggg
      421 atcgacgtcg caggcatatt cgcaaactgt aaacttcccg gcgttgccaa atgaagatgg
      481 cacacaggtg ttcttcagcg agccgttcga gctcaagggt aggcgcaaca tcagaatcgt
      541 gggccagtcg ccggtgcaga acacgtgggt gtatctcgaa ggtgatttga tcaacgacga
      601 gacgggcgtg gtccagtcgt ttccgatcga catctcttat tatcagggtg ttgaggatgg
      661 agaatcgtgg tcggaaggcg ggcagagtga ttcggcgttc acgtcatcgc tgccggccgg
      721 gagatatgtg ttgcggttag aagggcagtg ggagaagtgg tcgcaaccgg ccgtggtgtc
      781 tgtgcaagtc gagcagaacg tgacgcgtgg gttcaacttt ctaatcgctt taattgtgtt
      841 atccattggc ccagtgctca tgattgtcta ccacatcggg ttcgagcgac gacgctggtc
      901 ggaaagtatg tttggtggtg gtgacgattc agacgacgat gatgattaga caggatttac
      961 aagatttttc aggatttaca tgtttatcct gaaaaatctt gtaaatcctg tcaaaggctt
     1021 ttgttgagga gtatcaaatg ctcaaagcac tctacacaat cttcgctgtt ggcgtggtga
     1081 gtctttacag cgccgcggcg ttgctgggct gggaaatcag cggcggctcg cgacggcaac
     1141 taccacccga tgcacgccaa aatggctatc gctcgttcca cttctggcac tatggcttac
     1201 acggaggaaa atgatgcttg atgcaactag cttcggactg ttcattccga tggagaatct
     1261 gctgccggtg gtcgtcacga cgctcgtatt cgtggtcatc ggcctcattg ttttcgcgct
     1321 cgcgtttctg gtgattgcca aagcggcgcc gttctcggtc cgcaaagaga tcgaagacga
     1381 tcaaaacatc gcgctcgcga tcgtgatcgc agcagtgatc ctcggcagcg ccctgatcat
     1441 cgccgcagcc gtccactaag aagatttaat ttgaactgag aaagatgaaa cggccccctt
     1501 tactctttct caacgttctc atcgtcgcga cctgcggact gatctacgag ctgctggcgg
     1561 gcactcttgc aagctacgtt ctcggcgact ccgtcacaca attctcactc atcatcggta
     1621 tttatctgtt cgcgatggga gtcggttcgt ggctctcacg cttcatcgac aagggattgg
     1681 cgcgccgctt tgtcgacatc gaattggcag tcgccgtgtt aggcggcttt tccgcgccga
     1741 ttctgtttct cacgttctcg cgcgtcagtt acttctacgt cgttctttac ttcatcgtct
     1801 ttgcgatcgg cgtgttcgtc ggcctcgaaa tccctttgct gcttcgcatt ctcaaagacg
     1861 agatcgaatt taaagagctc gtctcgcgtg tgcttgcgtt tgattacgtc ggcgcactcg
     1921 tcgcttcgat tctgtttccg attctcttcg ttccacgact cggcctcatt cgaacgtcgc
     1981 tcatcttcgg aatgctgaat gccggtgtcg ctttgtgggg tacgtggatg ttgcgaccgc
     2041 tgatcaaagg acgcatcacg ggactgcgcg tgagagccat cgttgtcatg gtgttgttgg
     2101 tgatcgctct cgttaaggca aaccggatca cgtcgcttgc cgaagacgag atgtttgccg
     2161 atgagatcgt ctacacgaaa gacacgcctt accagcgcat cgtgatcacg cgcgggcgcg
     2221 cggggtttca gttgtttctc aacggcaatc tgcagttcag ctcgactgac gagtatcgct
     2281 atcacgaggc gctcgtgcat ccggccatgc ttttgtcgaa caatccgcga cgggtgctgg
     2341 tgttgggtgg cggcgacggg ctcgcgttgc gtgagatcct gaagtacccg tcggttgagc
     2401 acatcacgct tgtcgatctc gatccggaga tgacgaagct ctcgagtgcc tttcccttgc
     2461 tcgcaaagtt gaaccagaac tcattcaatg atccgcgcgt ccacgtgatc aatgaagatg
     2521 cttttatttg gctcgagaag acgaacgagc cgctctacga cgctgcgatc gtcgactttc
     2581 ccgatccaaa ctcgttcgca ctcggcaagc tctacacgac gcgcttttat cgtctgctga
     2641 aatctcgctt gactgagaac gcgggcgtct cggtgcaatc gacgtcgccg atgtttgctc
     2701 gcaattctta ctggtgcatc atccgcacga tcgaggcagc gggtttcgtc gtcaggccgt
     2761 actacaccgc agtgccgagt tttggcctct ggggctatgc gctcgcccga tcgtcgtcat
     2821 tcgaggtgcc gcaaaatccg ccgccgggat tgaagtttct cgacgagcaa acactcgcag
     2881 cgatgttcac actctcgaaa gacatcgagc ccgtgccggt cgagatcaac cgcctcgaca
     2941 accaggtgct ggtgagatac tacgagggcg agtggcgcaa atttgaatga agccacgaaa
     3001 aggcacaaaa agcacaaaaa aggctgttct caggctctta aactatcaga aatctttttt
     3061 gtgctttttg tgccttttcg tggcaagaaa tggctttgat gaataggcgt gaaattcttg
     3121 cggcgtttct cggtgtgcca tttgcgctcg cggcgtgtcg gaggaatgaa gagctgccgt
     3181 tgcccgatgg tgaaattgtc ggcgcgtcag atgtatttgg acatcggttg cgggatggat
     3241 tgcacgtgga gattcctcaa gacgcgtggt ccaatgttcc gttagtgata gttggcggcg
     3301 gtgtcgctgg cctaacagcc gcgtggcgac tgcgtaacaa cggctttaca aatttcactt
     3361 tgatcgagct cgaaagcgcg cccggtggca cgtctcgctc cggctcgaat cgactgatct
     3421 ccttcccgtg gggcgcgcac tacattcccg cgccgttgaa agagaacgcc gcgctcgtct
     3481 cactgctcga cgaaatgggc gtcgtcgaag gcaaagacaa agacggcgaa ccaatcatcg
     3541 gcgagcagtt tcttacccgc gatccggaag aacgtgtctt ttataaagga cgctggtacg
     3601 aaggtctcta tcttcacgcc ggtgaaagcg ccgacgatca gcaacagttt cagaaattca
     3661 atgctgaagt agataagtgg atcgcgtggc gcgatggaaa aggacgccgc gcgttcacgc
     3721 taccggtatc ggcgtgttca gacgatgccg aagtaacgtt actcgatcgt gtttcgatga
     3781 gcgagtggat gggcgcgcgc ggactcacga gcgcgcggct acgctggtgg gtcgattacg
     3841 cgtgtcgtga cgactacggc atgacgctcg atcacacgag cgcgtgggca ggcttgtttt
     3901 acttctgttc tcgagcaccc gcacctaaaa ccgagtcgca gccgttgatc acctggcctg
     3961 aaggtaacgg acgcctggtc caacacctgt ttgagaaggc gaaagataac attcaactcg
     4021 atcgtgctgc agttgagttg ataccgacgg acaatggtgt tgacgtcatc acgatcgatc
     4081 acaatggacg agagccacgc ggctttcatg cgcagcgcgt gatcttcgca gcgccgcagt
     4141 tcgtcgcgcg gtatgtggtg cggccgtatc gcgacaaccc gccgccgcac atcgctgatt
     4201 ttcagtttgg ggcgtggatg gtggcgaacc tgacgttgaa agatcggccg aaacccggct
     4261 cgcagcgtga ttttccgctt gcgtgggaca atgtttttta cgagagtccg tcgctcggat
     4321 atgtggtggc gacgcaccag cgcggcctcg atcgtgggcc aaccgtgctt acttattact
     4381 acccgctcgt tgatgaaaac ccgcgcgtcg gacggacgcg tttgttagag actgactggc
     4441 gcggctgggc tgaagtgacg ttgagcgatc tttcacgcgc acatcaggac attcgtgggc
     4501 tcgtggaacg tttggacgtg atgcgttggg gccacgcgat gatccggccg cgaactggat
     4561 ttatgtgggg cgccgctcgt cgtgaaggtg cgaaaccgtt tcgcgggatc cacttcgcac
     4621 actcagagct gagtggtgtc gcgctgtttg aagaagcgtt tgataatggt ctacgcgtcg
     4681 gaaatgaaat cctgtcaatc ctgtaaatcc tggctaacaa gatg
//
LOCUS       DASSFQ010000056        12584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1962859, whole genome shotgun sequence.
ACCESSION   DASSFQ010000056 DASSFQ010000000
VERSION     DASSFQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962859"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2751
                     /locus_tag="VFH96_01725"
     CDS             <1..2751
                     /locus_tag="VFH96_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6972710.1"
                     /translation="IVNLIGEGGMGEVYLATDTILGRRVALKVLPTFVSKDPERLRRF
                     TQEARAASRLSHPNVCVVHEIGETDDGRPFIAMEYVEGMTLRERIRNQAMKLGDVFDI
                     AIQIAEGLIAAHEAGIIHRDIKPENIIIRSEGYVKILDFGLAKLTERHQSATTTTMPT
                     LLFHSTPGVVIGTAAYMSPEQARGVAVDERTDIWGLGVVLYEMASGRAPFTGETPTDV
                     VVAIVERDQPPISQHVEGAPPELERIVKKTLRKDKNERYQIVKELAIDLRSLRRELEM
                     NSMLERSIMPATGSSDYARASSGRDKKVETNELKQARTTIHKPVGSRAWIGLAALAAI
                     VIALTGYGLYKMRARTMRIPFERVEVTKLTTNGNALMGAISPDGKYVAYVTGESGKES
                     LWLRQVDINSNAQLVAPREGHYLGVAFSPDNNYVYFGYAESARNDASQIFRLPVLGIG
                     AAAMKIEAHEALASLSHDRKRIAFIRFDRQAQTDSLIVANADRSGEQLIGTRKWPQHY
                     GWDPLSRPEWTTDDRTLMLPIVNAAPSYNGDTSSSYTAALLEKDLGNGAERTIPLSPQ
                     KFDELGRVTVHPDGNGVILLGKAHGAAFIQIWQLLRDGTQRTLTSDLSDYRELSLRAD
                     GSALITVQTQTLSRLWQLRPNESKPTPVGSGTSRYYDLTIAPDDKILYASDASGIADI
                     FEIAPTSGEARPLTSAGGRNYAPVVSPDNRYVVFHSNRTGVFQVWRINRDGSSPKQLT
                     FAETESTWPTFSPDGKWIIFQHSDGNNPYTLWRVSIDGSTPEKITDGMAIKPAVSPDG
                     KLIAFWYNDGKKNSRWRLQVINFEGGATFNTFDVAETVQVQWDTPLHWSLDGKYLVYV
                     DHAGGFDNLWGQPIEGGALKQLTKFDEGKIFAFGWLKDGSLVTSRGVITSDVVLIKDA
                     GR"
     gene            2959..4062
                     /locus_tag="VFH96_01730"
     CDS             2959..4062
                     /locus_tag="VFH96_01730"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET6972711.1"
                     /translation="MAEKRVGVLTGGGDAPGLNPAIKGLVYRGSELGLEVIGLFDGWR
                     SLLNPLPDTLPLNREAVRRWDRDGGTNLGSSRTNPFKAEGTTDRSQEVIENIERLKLD
                     ALVACGGEDTLGVAAGLAQKGVRVVGVPKTIDKDLAGTDYTLGFDTALRNVTEILERS
                     RTPAGSHGWVQVVEVMGRNAGHLALWSGVAGQAHMILIPEHPFRYQRVFELLSARLGD
                     SDPLRRGTQRPRYSVIVVAEGARVEGGEMVTLDDRRDAFGHERLGGVGEVLAKTIAAQ
                     TPYESRAVVLGHPQRGGAPSPIDRIMGMMFGARAAEAVADGRFGTMVSARGIAPACDL
                     SLVDLSNVQGKMTLVDVDRYYDTERYHLKGIGM"
     gene            complement(4163..5665)
                     /locus_tag="VFH96_01735"
     CDS             complement(4163..5665)
                     /locus_tag="VFH96_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET6972712.1"
                     /translation="MEEDSTDKIFAVNPPPPDYNPVKQAIARADELGEKQIATALGYF
                     STMPASTGIIANPVLHHTPVDCNGTTITLDIRSFTIRGTVPESDIVAGKIGPIDVIYT
                     GLFAAAGDTKELAAFIDQKYFQSLSGSLIEEVAAFVKKFPGLGPEVAMHHFSILAKAK
                     LGREEHQDRGMHDERDPESMLIEMISVHMANVAVGGISTYMNHLLFDNPQITEAQLAS
                     STRQFIDDESKEKRNAFQVSYSLILGHHANEHEREILERMGTIQIHHGSAGSSMVARY
                     LATLHTFSVADFFIASQMALDGARHFGAIHDMSDFVQELEPLSSEQRSERIKEKMLTG
                     GLPTFGHPEIAAAGRGNQVQQDPRPAIYIDPVFKAIDAGQLSISERQHERLAIARRIY
                     LTAFVNGVLKPGKEDSPPLRLTANTDFGAWCVQEALGVEEPDRTLLTYIFRGFGWMMD
                     AREQLQQKMIRPVIAPDPKIIPKSSDDPTIPDLVARVHKRLTQDEPFKAH"
     gene            5907..7334
                     /locus_tag="VFH96_01740"
     CDS             5907..7334
                     /locus_tag="VFH96_01740"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET6972713.1"
                     /translation="MPRASLIATLFEPPSSDGAELTALPEAVEWLEVRADRIGDIDPE
                     WLRDHFKGRLLYSFRTEDCTALNRSERLKTAARFYDRVEIEAETDLSKDLLEVIPAEK
                     RLLSWYGRVNDLSQLNDRFAQLSSVPAAAYKIVTKAETISEEFLPLSLLKSLNRTDAI
                     AYCKGPLGFWNRLVALHLGSPAIFGLVSGGSAADMEPSIGKLIADYGLPEVHPAKELF
                     AIIGNPIFHSLSPRLHNASYRALNHAALFVPLRVHSFAEFWREFVTSRKLDQFGMPIN
                     GMTVASPHKEEAVQTAKTVSGMVRQTEAANILVRNNGWWTADTTDPDIVYSVSVKEKR
                     AAVIGCGGAGRAIAAALVKSGAGVTLINRGAERGEHAAALLGLPYIPLRDFNAKGYDI
                     VVNATPVGRDTDEAPFAVEQLNNGAVVIDLVYGSRPTPLVDTTRARRQLAIDGRDVLV
                     TQVSQQFRLMTGKDMPVDLAREILL"
     gene            7513..8742
                     /locus_tag="VFH96_01745"
     CDS             7513..8742
                     /locus_tag="VFH96_01745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6972714.1"
                     /translation="MITTDSKNDAYAQLDLSAEQQDLINRVAVLARENFAARAAEYDR
                     AAAFPAEDFDDLFRAGLLAPVVPKEHGGHGLGPYKGEVFPLWMMTKEIARADMSLARC
                     WEGHVNSLVLLDGMATEEQKTRWFKGVVERGEKWVAWSGEPQARKPGEQARFGTNVEK
                     VDGGYIIDGNKAFATSAGGAQWAILLVNTAGPGGVRHVAAASGDSVLMMACNLSDPSV
                     EIDTSWWDPIGMRGTASHMVHFRNTFIPDDDLIGYPGQYLKEGWQTCFIPHYAATFLG
                     AAEAAYDYGLNYITTQNKINDPYVQHHLGQMSVNVETAHLWLRHVARLWETGKYLEAQ
                     IAGSRARHVVEHLAEDTVKQCIRACGARCLIRPSSLERVYRDLSFYVRHDNDDQILAT
                     IGKSILGLTHDPSFYKP"
     gene            8779..10338
                     /locus_tag="VFH96_01750"
     CDS             8779..10338
                     /locus_tag="VFH96_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972715.1"
                     /translation="MHITSQRNFKLQIWGLALGYFAFYAPYSFLIKIVTSKLWPGVGA
                     SVSGFRLLPAVVIGTAITLPLIITFKGWWKYARTRKFMGAGIPIPSGLVVLSGLGTAI
                     IIGTTTLAFSFAGVSILFALLLMRGGVLTIAPLVDSVFRRRVRWFSWVALGLTVPALL
                     IALIDVNNYRLTLVAALTIGAYLSGYLLRLPCVTRMAKVKDTHITYRYFVEEALVAII
                     FLVALPVIFSLIGRGELMTEVRQGFVEFFAGGVTIPALLIGALYACLYCFGTLIYLDC
                     RENTFCIPLNRGSSLLAGVLATVTLALLFGQKPPSAAQLSSAALIVIALLFLSPLHHF
                     DRYLPKLKEALLGLKPGVRIPTAQPATAIPRLILFVCSGNTCRSPMAAAIANDDIAKR
                     LGIPFKSLETVNVRALSAGVSARAGAPLTVEAAAVLRSLSVPVQPHAARNLTPELAAQ
                     SELIFCMTKAHRKAVVELLPEVAGKTYCLSTEVDVDDPIGKGMAAYVDCARQIQRLVQ
                     LRLNELRLVGT"
     gene            10340..12184
                     /locus_tag="VFH96_01755"
     CDS             10340..12184
                     /locus_tag="VFH96_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972716.1"
                     /translation="MLARVSPIIDTLGPLSFAEFQAYRSPGTVLWCNFDLLRELGFTV
                     PETNQLTPELNQQLLAAFSLRAVNGDDLNGHELLTLYADKYGGEGVSPALGAGRAGFL
                     AHGNLYVKGLGFTPLFRHNDPDDFAHSHGGVHLEDCLSEAVFGEVTHNLFALGSNRIV
                     AIIDQGKHVTEPSGRRRHIALAVRAGAQLRPGHLLARRARGSRSALETFVSITRATGQ
                     LVTTSDAPDVSATMLRVIDDHAQTAADSFRWRMIHGALSPSNMDISGAMLDLPTQSTQ
                     PRTAPIFLLDYASSHFGTEHKERGAHLAEMYRRVLRTTDATTRERFNAKWVNVPNVMD
                     EAYEKHVQVRLLAATGIKTEVARRIQAEHSDVGKSFAKLLMEMAALKNVGSACVARKV
                     VADVSVVDVFNLLGKAPPEYFSERLSRGRVVKHLKPVYKGNRFQVAKKRVYVDSLARE
                     FVRLYGDVMNLCVAYTGDYYDSVEGMKASIAARASFENEPIDALYCHSLYQKFNEAIV
                     QYKASGKTKRISEVIDTTIVQSLRSVDGLLAQGDWRILEDGGIEIERRTIRGVSYAVR
                     AWDDEAQTRRVCVNGVEFKASGIVGRLKGGYVFAIPDRQELRQFRDVR"
     gene            12261..>12584
                     /locus_tag="VFH96_01760"
     CDS             12261..>12584
                     /locus_tag="VFH96_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972717.1"
                     /translation="MWSIAIYTGISPFDLQPAPPVLTKAEVTDIPADFVADPFMVERD
                     QTWYMFFEVMHTETKLGEIGLATSNDALNWTYDRIVLKEPFHLSYPHVFEWQNQYYML
                     PEMLRA"
BASE COUNT         2733 a         3487 c         3563 g         2801 t
ORIGIN      
        1 atcgtgaacc tcatcggtga aggtgggatg ggcgaagtct atctcgcgac cgacacgatc
       61 ctcggccgtc gcgtcgcgtt gaaagtgctc ccaacattcg tcagtaaaga tccggagcgc
      121 ctgcgtcgtt tcacacagga agcgcgcgcc gcatcacggc tgagtcaccc gaacgtctgc
      181 gtggtccatg agatcggcga gacagacgac ggacgcccgt ttatcgcgat ggaatacgtc
      241 gaaggcatga ccttgcgcga gcgtattcgc aaccaggcga tgaaactcgg cgacgtgttc
      301 gacatcgcga tccagatcgc cgaaggcctg attgccgcac acgaagcggg tatcatccat
      361 cgcgacatca aacccgaaaa catcatcatc cgttccgaag gctacgtgaa gatcctcgac
      421 ttcggactcg ccaaactcac cgagcgacac cagagcgcga cgaccacaac gatgccgaca
      481 ctcttgtttc actccacacc gggcgtcgtg atcggaacgg cggcttacat gtcgccggaa
      541 caggcgcgcg gtgtcgctgt cgacgagcgc acagacatct ggggactcgg cgtcgtgctt
      601 tacgagatgg cgtcaggtcg tgctccgttt accggcgaga ctccgaccga cgtcgtcgtg
      661 gcgatcgtcg agcgtgatca accgccgatt tctcaacacg tcgaaggcgc gccgcccgag
      721 ttggaacgca tcgtcaaaaa gacgctgcgt aaagacaaaa acgagcgcta ccagatcgtg
      781 aaagagctcg cgatcgatct gcgcagtctg cggcgcgagc tggaaatgaa ctcgatgctc
      841 gaacgatcga tcatgccggc aacgggttca agcgactacg cacgcgcctc atctggtcgc
      901 gataaaaagg tcgagacgaa tgagctgaag caggcgcgca cgacgattca caagccggtc
      961 ggtagtcgcg cgtggatcgg tctcgctgca cttgcagcga tcgttatcgc actcacaggc
     1021 tatggccttt acaagatgcg cgctcgaaca atgcggatcc cgtttgagcg ggtcgaagtc
     1081 acgaaactga cgacgaacgg aaacgccctg atgggcgcga tctcgcccga cggcaagtac
     1141 gtcgcttacg taaccggcga gagcgggaaa gaaagccttt ggctgcgaca ggtagatatc
     1201 aatagcaacg cgcagctcgt cgcaccgcga gagggtcatt atctcggcgt cgctttctcg
     1261 cctgataaca actacgtcta ttttggttat gcagaatctg cgcgcaacga cgcgagtcag
     1321 atcttccgtc tgccggtact cggaatcggc gccgcggcga tgaagatcga ggcgcatgag
     1381 gccctggctt ccctctcgca cgatcgcaag cggatcgcgt tcattcgatt tgatcgccag
     1441 gcgcaaactg attcactgat tgtcgcgaac gctgaccgca gtggcgagca gcttatcggc
     1501 acacgcaaat ggccacagca ctatggctgg gacccactgt cgcgacccga atggaccaca
     1561 gacgatcgga cgttgatgtt accaatcgtc aatgcggctc cttcttacaa tggcgacaca
     1621 tcgtccagtt acacagccgc actgctggaa aaggatctgg gcaacggggc ggagcgcacg
     1681 attcccctgt cgccgcaaaa gtttgacgag ctgggccggg tcaccgtgca tccggatggc
     1741 aacggcgtga tcttgttggg aaaagctcat ggcgcggcat tcatacagat ctggcagctc
     1801 cttcgagatg gaacacaacg cacgctcaca agcgatctca gcgattatcg tgaattaagt
     1861 ttgcgcgcag atggcagcgc gctcatcacg gtgcaaacgc aaactctctc ccgactgtgg
     1921 cagcttcgtc caaatgagtc gaaaccaact ccggtcggtt ccggcacgag ccggtactac
     1981 gatctcacga tcgcacctga tgacaagatc ctttatgcgt cggatgcgag cggtatcgct
     2041 gacattttcg agatcgctcc aaccagcggt gaggcaaggc cgctaacgtc tgcaggcggt
     2101 cgaaattatg cgcccgttgt ttcgcctgac aatcgttacg tcgtcttcca ctcgaaccgc
     2161 accggtgtct ttcaggtttg gagaatcaat cgcgacggca gcagtccgaa gcaattaacg
     2221 ttcgcagaga ccgagagcac gtggcccact ttctctcctg acggcaagtg gatcatcttc
     2281 cagcactccg atgggaacaa tccgtacact ctctggcgtg tctcgatcga cggcagcacg
     2341 cctgaaaaaa ttactgacgg aatggcgatc aagccggccg tctcgccgga tggaaaattg
     2401 atcgcattct ggtacaacga tggaaagaaa aactcgcgct ggcgtttgca ggtgatcaac
     2461 ttcgaaggtg gtgcgacttt caacacattt gacgttgcgg agacagtgca ggttcaatgg
     2521 gacacgccgt tgcactggag tctcgatggc aagtatctcg tttacgtgga ccatgcaggc
     2581 ggtttcgaca atctctgggg acaaccgatc gaaggcggcg cactgaagca gttaacaaaa
     2641 ttcgatgaag gaaagatttt tgcatttggc tggttgaaag acggcagcct cgtgacctcg
     2701 cgcggcgtga tcacgagcga cgtggtgctg atcaaagacg cagggcgctg attgagcccg
     2761 cgaagcgggc gcaagcataa agcctggggc gcgagcccca ggttctcaaa ccaaaagagc
     2821 aacgagcccg cgaagcggcc gacagctgcc gcccgcctcg cgggctggaa aattgatttt
     2881 tcatcctatc cctagggctt acgccctagg ctttatgctg acacctgctt cgcaggttaa
     2941 gaaaaggaaa atttatagat ggcggaaaag cgtgtcgggg ttttaactgg cggcggtgat
     3001 gcgccgggtt tgaaccctgc tattaaaggt ttggtttatc gtggctcgga gctcgggctc
     3061 gaggtgattg gactattcga tggctggcgc agccttttga atccgttacc tgatacgtta
     3121 ccgctcaatc gtgaggcggt gcgtcgctgg gatcgcgatg gcggcacgaa tctcggttcg
     3181 tcacggacga atcctttcaa agcagaagga acaacggatc gctcgcagga agtaattgaa
     3241 aatattgagc gattgaaact ggatgcgctc gttgcctgcg gcggtgaaga cacactcgga
     3301 gttgccgctg gtctcgcgca aaagggtgtg cgcgtcgtcg gtgtgcccaa aacgatcgac
     3361 aaggatctcg ccggcacgga ttataccctc ggcttcgata ccgcgctccg caatgtcacg
     3421 gaaattcttg agcgatcgcg cacgccggcc ggatcgcatg gttgggtaca ggtcgtcgag
     3481 gtgatgggca gaaacgccgg gcatctcgcg ctgtggtcgg gagtcgccgg acaagcgcac
     3541 atgatcctta ttccggaaca tccgtttcgc taccagcgag ttttcgagtt gctcagtgcc
     3601 agactcggcg acagcgatcc tttgcgacga ggaacgcagc gtccgcgtta ttcggtgatc
     3661 gtcgtagctg aaggagcgcg tgtagaaggt ggcgagatgg taacgcttga cgatcgacgt
     3721 gacgcatttg gacacgagcg actcggcggc gtgggtgaag tgctggcgaa aacgatcgca
     3781 gcgcagactc cgtatgaatc acgcgcggtc gtgcttggtc atccgcaacg tggtggagca
     3841 ccaagcccga tcgatcgaat catgggaatg atgtttggcg cgcgcgcggc ggaggcggtg
     3901 gctgacggtc gtttcggaac gatggtctcg gctcgcggga tcgctccggc gtgtgatctc
     3961 tcgctcgtcg atctttcaaa cgtgcagggg aagatgacgc tcgtagatgt cgatcgttac
     4021 tacgacaccg agcgctatca cctcaaaggc atcggcatgt agtacacaaa aagcacaaaa
     4081 agcacaaaaa tgatcggagt aatcacgcgt cgtggtcctt ccgatcattt tttgtgcttt
     4141 ttgtgccttt tgtgtacagc ttttagtgag ccttgaacgg ctcgtcttgt gtcagtcgtt
     4201 tatgaactcg cgcgacgaga tcaggtatcg tcggatcgtc gctcgacttt ggaatgatct
     4261 tcggatcggg cgcgattacc ggtcggatca tcttctgctg taactgctcg cgcgcgtcca
     4321 tcatccatcc gaagccacgg aagatgtaag tcaacagcgt ccgatccggc tcttcaactc
     4381 ccaacgcctc ctgcacacac cacgcaccaa aatccgtgtt cgcagtgagc cgcaacggcg
     4441 gactgtcttc ctttcccggc ttcaacactc cattcacgaa cgccgtcaga taaatccgtc
     4501 gcgcgatcgc gagccgctca tgctgccgtt ctgagatact caactgaccc gcatcgatcg
     4561 ccttgaacac cggatcgata taaatcgccg gtcgcggatc ctgttgtacc tgattgcctc
     4621 tgcctgccgc agcgatctcc ggatgaccaa acgtcggaag tccgccagtc aacatcttct
     4681 ccttgatccg ttcactgcgc tgctcggacg acagcggctc gagctcctga acgaagtctg
     4741 acatgtcgtg aatcgcaccg aagtgccgcg cgccgtcgag cgccatctgc gatgcgatga
     4801 agaagtccgc gactgagaac gtgtgcaacg tcgccagata acgcgcgacc atgctcgagc
     4861 ccgccgagcc gtgatgaatc tgaatcgtac ccatacgctc gaggatctcg cgctcgtgct
     4921 cgttcgcatg gtgtccgaga atcaaactgt aactaacctg aaacgcgttt cgcttttctt
     4981 tcgattcgtc atcgataaac tgccgcgtcg agctggcaag ctgggcttca gtaatctgtg
     5041 gattgtcgaa cagcagatgg ttcatgtagg tcgaaatgcc cccaaccgcc acattcgcca
     5101 tgtggaccga gatcatttcg ataagcattg attcgggatc gcgctcgtca tgcatgccgc
     5161 gatcctggtg ttcctcacga ccgagcttcg ctttagcgag aatcgaaaaa tgatgcatcg
     5221 cgacttccgg accgagtccc ggaaacttct taacaaacgc ggccacctct tcgatcaacg
     5281 aaccagaaag actctgaaaa tacttttgat ctataaaagc cgcgagctct tttgtgtcgc
     5341 ctgctgcggc aaaaagtccc gtataaataa cgtcgattgg accaatctta ccggctacga
     5401 tgtctgactc gggcactgtg ccgcgaatcg tgaacgatct gatgtcgaga gtgattgtgg
     5461 ttccattgca atcgaccggt gtgtggtgca acaccggatt ggcgatgatg ccggtcgagg
     5521 cgggcatcgt ggaaaagtaa ccgagtgcag tggcgatctg cttttcacca agctcgtctg
     5581 cccgcgcgat cgcctgtttt acgggattat agtcaggcgg tggcgggttc accgcgaaaa
     5641 tcttgtccgt cgaatcttct tccatgttag gccgaattcc ttttttggtc taagaatagc
     5701 gattcttgcg cgtggcaaca actctctgcg gctctctgcg tatctctgcg ttctctgcgt
     5761 taagaagtat tcaacgcaga ggccgcagag atacgcagag ggccgcagag attttgtgga
     5821 ctaataactg caagcgaagt atagtagccg ctcgctttta acgtctgaac ttctcacgca
     5881 atcactagag taaagagtaa tgaactatgc cacgagcctc actaatcgcc actctttttg
     5941 agcctccgtc atcggatggc gcggaattga ctgcgttgcc ggaggcggtg gaatggttgg
     6001 aagtgcgcgc cgatcgcatt ggcgacatcg atcccgagtg gctgcgcgat cattttaaag
     6061 gccgactgct gtattccttc aggactgaag actgtactgc attaaatcgt tctgagcgtc
     6121 tgaagactgc cgcgcggttt tacgatcgcg tggagatcga ggccgagaca gatctttcca
     6181 aagatttgtt agaagtgatt ccggccgaga agcgtctgct ttcctggtat ggtcgagtta
     6241 acgatctatc acaactgaac gatcggtttg cgcaactctc gtctgttccc gcagccgcgt
     6301 acaagatcgt cacgaaggcc gagactatct ccgaagagtt tctcccgctc tcgttattga
     6361 aatcactgaa tcgcacagac gcgatcgcat attgcaaagg tccgctcggt ttctggaatc
     6421 gactggttgc gttgcacctc ggctcacccg cgatcttcgg tctcgtctca ggcggttccg
     6481 cggcggatat ggagccgtcg atcggcaagt tgatcgctga ctatggtctt cccgaagtgc
     6541 atccggcgaa agaattgttt gccattatcg gcaatccgat ctttcattcc ctctcgccgc
     6601 ggttgcacaa tgcgtcgtat cgcgcgctaa atcatgcggc gctgtttgtg cctttgcgcg
     6661 tccattcgtt tgcagaattc tggcgcgagt ttgtcacttc gcgcaagctt gaccaattcg
     6721 gcatgcccat caacggtatg actgtcgctt caccgcacaa ggaagaagca gtgcagaccg
     6781 caaagacagt cagtggcatg gtgcggcaaa ctgaagctgc gaacattctc gtcagaaaca
     6841 acggctggtg gaccgcagac acgaccgatc cggatatcgt ctacagtgtg agtgtaaaag
     6901 agaaacgtgc cgcggtgatc ggctgtggcg gcgccggacg cgcaatcgcg gcggcgctcg
     6961 tcaagtcggg cgcgggcgtg accctgatca atcgtggcgc ggaacgcggt gaacatgccg
     7021 cagcgttgct tggcttgccg tatattccct tgcgcgactt caacgccaag ggttacgaca
     7081 tcgtcgtgaa tgcaacgccc gtcgggcgcg acacggatga agcgccgttc gcagtcgaac
     7141 aactcaacaa tggtgcggtc gtgatcgatc tggtctacgg ctcgcgcccg acacctttag
     7201 tcgacacgac gcgcgcccgc cggcagcttg cgatcgacgg tcgcgacgtc ctcgtcacac
     7261 aggtaagcca gcagttccgt ctcatgacgg gcaaagacat gcctgttgat ttagctcgag
     7321 agatattact gtagggatgg ccctccgtgg ccacccgttc gttacgcaat cagcgcgggt
     7381 ggccacagag ggccacccct acagttcggc ccgaacatca aattcaatga tcagcatcga
     7441 atccaagtcg acccgagtgc caaattcaat aatcggtacc gattccaaag tcggtccaca
     7501 tggccaaaat caatgatcac taccgattca aagaatgacg cctacgcgca actcgatctc
     7561 agcgccgagc aacaggatct gatcaatcgc gttgccgtgc tggcgcgcga gaattttgca
     7621 gcgcgcgcgg ctgaatacga tcgcgccgcc gccttccctg ccgaagactt cgacgatctc
     7681 tttcgcgccg gactgctcgc tccggtcgtg ccgaaggaac acggcggaca cggtctcggt
     7741 ccatacaaag gcgaagtctt cccgctctgg atgatgacca aagagatcgc gcgcgccgac
     7801 atgtcgctcg cgcgttgctg ggaaggtcac gtcaactccc tcgtgctgct cgacggcatg
     7861 gccaccgaag aacaaaaaac gcgctggttc aaaggcgtcg tcgagcgtgg cgagaagtgg
     7921 gtcgcgtgga gcggtgagcc acaggctcgc aagcccggcg aacaggcacg cttcggcacg
     7981 aacgtcgaga aagtcgacgg cggttacatc atcgatggca acaaggcttt tgcaacgagt
     8041 gccgggggcg cgcagtgggc gatcttgctg gtcaatactg ctggaccagg cggcgtcagg
     8101 cacgtcgctg cggcgagtgg cgattcagtg ttgatgatgg cgtgcaacct ttccgatccg
     8161 agcgtcgaga tcgacacctc gtggtgggat ccgatcggca tgcgcgggac ggcgagtcac
     8221 atggtccact tccgcaacac gttcattccc gacgacgatc tcatcggcta tcccggacag
     8281 tatttaaagg agggctggca aacgtgcttc attccgcatt acgccgccac cttcctcggc
     8341 gccgccgaag ctgcttacga ttacgggctc aactacatca ccacgcaaaa caagatcaac
     8401 gatccgtacg tgcagcatca ccttggccag atgtcagtaa atgttgagac ggcgcatctg
     8461 tggctacgac acgtcgcgcg actgtgggag acgggcaagt atctcgaagc gcagatagca
     8521 ggcagtcgcg cgcgacacgt cgtcgagcat ctcgcggaag acacggtcaa gcaatgcatt
     8581 cgcgcttgtg gcgcccgttg cttgattcgc ccgagttcgc tcgagcgcgt ttatcgagat
     8641 ctgtccttct atgttcgtca cgacaatgac gatcaaattc tcgcaacgat cggtaaatca
     8701 atactcggtc tgactcacga tccatcgttc tataaaccgt agcggtggtt cgtgaactcg
     8761 ctcaccaggg ggtaagacat gcatatcact tctcagcgta actttaagct tcaaatttgg
     8821 ggcctggctc tcggctactt cgcgttttat gctccttaca gttttctaat caagatcgtc
     8881 acctcaaaac tctggcccgg tgtcggcgcg tctgtgtccg gcttccgctt gttgccggct
     8941 gtcgtcatag gtacggcgat tacccttcct ctcatcatca cgttcaaagg atggtggaag
     9001 tatgctcgta ctcgtaaatt catgggagcg ggcattccca tcccaagcgg actggtcgtt
     9061 ctttccggcc tgggaactgc catcattatt ggaaccacaa ctctcgcttt tagctttgcc
     9121 ggagtgtcaa tactattcgc gttactgctg atgcgtggag gagttttgac catcgcgccg
     9181 cttgtcgata gtgtatttcg tcgtcgagta cggtggttct cgtgggtcgc ccttggccta
     9241 acggtccctg ctttactgat cgctctcatt gatgtaaaca attaccgtct caccttagta
     9301 gccgcactca cgattggcgc ttatctttca ggttacctgc tgaggctgcc atgcgtcacc
     9361 cggatggcta aagtcaaaga tactcatatt acttaccgtt acttcgttga agaggccctc
     9421 gtggcgatca tctttctggt tgcgctacca gtaattttta gcctgatcgg tagaggtgaa
     9481 ttaatgacgg aggtccgtca gggttttgtg gaattttttg cgggcggcgt gactatcccg
     9541 gcattgttga ttggagcact ttacgcgtgt ctttattgtt ttggcacact catctatctc
     9601 gactgccgtg aaaacacctt ttgtatccct ttgaatcgcg gttccagtct gctcgccggt
     9661 gttttagcaa ctgtcacact ggccctgctc tttggacaaa agccccccag cgcagcacaa
     9721 ctcagcagcg ccgcactcat cgtcattgca cttttgttct tatcaccact ccaccatttt
     9781 gatcgatatc tgccaaagct aaaagaagct ctacttgggt tgaagcctgg agttcgtatt
     9841 cccactgctc agcccgcgac tgcaatacca cggttgattt tgtttgtatg cagcggcaac
     9901 acgtgtcgct caccgatggc ggcggcgatc gcgaatgatg acatcgccaa acgacttggc
     9961 attccgttca aatcactcga gactgttaac gtgcgcgcct tgagcgcagg agtgtctgcg
    10021 cgcgccggcg caccgctcac tgtagaggcc gccgcagtct tacgctcttt gagtgtaccg
    10081 gtgcagcctc atgcagcgcg aaacctgacg cccgagctgg cggcacaatc ggagttgatt
    10141 ttttgcatga caaaggcgca tcgtaaggct gtcgtcgagc tgctgccgga agtcgcaggc
    10201 aagacctact gtctcagcac cgaagtcgac gtcgatgatc ccatcggcaa aggtatggca
    10261 gcatatgttg actgtgcgcg acagattcag cgattggtcc aactgaggtt gaatgagcta
    10321 cgccttgtag gcacttgaag tgttagcaag agtttccccc atcatcgaca cgctcggtcc
    10381 actctcgttc gccgaatttc aggcctacag atctcccggc accgtgctgt ggtgcaactt
    10441 cgatctcctg cgcgagctcg gcttcaccgt tcccgaaacc aatcaactca ctcccgagct
    10501 gaatcaacaa ctcctcgctg cgttttcact gcgcgcagtc aacggcgacg acctcaacgg
    10561 ccacgagttg ctgactctat acgctgacaa gtacggcggt gaaggtgtga gtccagccct
    10621 cggcgccggt cgcgctggtt ttctcgcaca cggaaacctg tatgtgaagg gtctcggatt
    10681 cacgccgctc ttccggcaca acgaccctga cgatttcgcc cattctcacg gcggcgtgca
    10741 tctggaggat tgcctgtctg aagcagtttt tggcgaggtg actcataacc ttttcgcgct
    10801 cggttcgaat cgcattgtcg ccatcatcga tcagggaaaa catgtgactg agccgtcggg
    10861 ccgacggcgt cacatcgcgc ttgcggtgcg tgcaggtgca caacttcggc ccggtcacct
    10921 gctcgcgaga cgcgcgcgcg gcagtcgctc agcgctcgag acgttcgtta gcatcacacg
    10981 cgctacgggg caactggtta ctacatccga tgcgccggat gtgagcgcaa caatgctgcg
    11041 tgtcattgac gatcacgcac agaccgccgc ggattcgttt cgctggcgca tgattcatgg
    11101 cgcgctcagc ccgtcgaaca tggacatcag cggcgcgatg ctcgacttgc cgacgcagtc
    11161 gacgcagccg cgcacggcgc cgattttcct gctcgattat gcctcgtctc atttcggcac
    11221 cgagcacaag gagcgcggag ctcatctggc ggagatgtac cggcgtgtgt tgcgtacaac
    11281 agatgcgacc acacgcgagc gcttcaatgc gaagtgggta aacgtaccga atgtgatgga
    11341 cgaggcttat gagaaacacg tgcaagtgag actactggcg gcaaccggga ttaaaactga
    11401 ggtggcgcgt cggattcaag cggagcattc ggacgtgggc aagagttttg caaagctgct
    11461 gatggagatg gcggcgttga agaatgtggg ttcggcgtgc gtggcacgga aggttgttgc
    11521 ggatgtttcg gtggttgatg ttttcaatct gctgggcaag gcgccgccag agtattttag
    11581 tgagcgtctg tcgcgaggca gggtcgtgaa acatctgaaa ccggtttaca agggcaatcg
    11641 atttcaggtg gcgaagaagc gcgtctacgt tgattccctg gcgcgtgagt tcgtgcggct
    11701 ctatggcgac gttatgaatt tgtgcgtggc gtatacgggt gattactacg atagtgttga
    11761 ggggatgaaa gcgtcgatcg ccgcgcgggc gagttttgag aatgagccga tcgatgcgct
    11821 gtactgtcac agtttgtatc agaagttcaa tgaggcgatc gttcaataca aagcaagtgg
    11881 gaagacgaag cgcatcagtg aggttataga cacgacgatc gtgcaatcgc tgcggagtgt
    11941 tgatggattg ttggcgcagg gtgattggcg aatcctcgag gatggtggca tcgagatcga
    12001 gagacggacg atccgcggag tgtcgtatgc tgtacgagca tgggacgacg aggcgcagac
    12061 gcgtcgagtg tgtgtgaatg gggttgaatt taaagcctcc ggtattgtcg gcaggctcaa
    12121 aggtggatac gtgttcgcaa tccccgatcg acaggagtta agacaattta gagatgtccg
    12181 ataagcttta gcttgtcgcg accttccgac aagctaaagc ttatcggaca tcttttgtgc
    12241 cttttgtggc taagttctat atgtggtcaa tcgcaatcta caccggcatt tcgccgttcg
    12301 atctccaacc cgcgccgccg gtcctgacga aggctgaagt caccgatatc cccgcagact
    12361 tcgtcgccga tccattcatg gtcgaacgcg accaaacctg gtacatgttc ttcgaagtca
    12421 tgcacactga aacaaagctt ggcgagatcg gcctcgctac cagcaacgac gcactcaact
    12481 ggacttacga tcgcatcgtt ctcaaagagc cgttccacct ctcgtaccca cacgtcttcg
    12541 aatggcagaa ccaatactac atgctccccg aaatgctcag agcc
//
LOCUS       DASSFQ010000057         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_21924, whole genome shotgun sequence.
ACCESSION   DASSFQ010000057 DASSFQ010000000
VERSION     DASSFQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21924"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..2672
                     /locus_tag="VFH96_01765"
     CDS             45..2672
                     /locus_tag="VFH96_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6972718.1"
                     /translation="MSDFSFGEPQQLKPHRAFSIALASLAFCIGGAALMGWIFNVDAL
                     KRIHPSLVNMKANTAICLMLVAIAVFLIQDSPTSKVRRRLALLLAAIVATVGAITFSE
                     HLFGWNSGLDQLLFHESVAEAGESFPGRMGVASSLNLVFFGLAISFLDARSTRWFRVS
                     TISVLAILAATLLVFLYYFYGIEHAEPLARYFTIAIHTVVAFVSLCAAILLARSDRDL
                     VASLLGNSPGAMVARRMLPAFLVVILLGWIRTIARSNGWISQGFATASFVLAILLLLG
                     ILIWLTALSLNRTDRERRRVDLAQRRAENRLDVLFRVSELIRTLRDPYELSYAVAEQV
                     GIHLDLRRCLFNETDIDRDLEIVHRDYCDGVVSVAGEHRISDYSSVTSQEMQKGQTVV
                     NNDSKTDPRTAADYQRSYEATGERAYVAMPLLREGRWVASLWASVDQPRVWTKEEVAL
                     VQAVAERTWTAVEKLRAEAERERLLQSVQEARDTAEKANQLKDEFLATLSHELRNPLN
                     VILGYSELLLRMREIEQSPRLVQMGEALRRNALSQSQLINDLLDLSRLQRGKISLNQE
                     TVSLPAIIDNAVETVRTDAATKGVEIRLQASDQLLLVEGDRLRLQQIAWNLLNNAVKF
                     TPAGGRIEISLNNEGDRAALVVADTGQGIDPSFLPHVFEMFRQADGSNRRRHGGLGIG
                     LALVRQLVHLHGGTINAESGGPNKGSCFTVCLPLLRETAPLSIPAPVATELSMFSGTS
                     FLVLDDSEDSIMMLTELLKLAGAHVVTATNGVDALRIASENEFDVILSDISMPEMDGF
                     EFLRRLRKIDGRQDVPVVAITGFGRSDDINRARDAGFYSHITKPLNLQALNTVLEQIL
                     NGHKRQSTDYADGKKQKSA"
     gene            complement(2695..>4081)
                     /locus_tag="VFH96_01770"
     CDS             complement(2695..>4081)
                     /locus_tag="VFH96_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6972719.1"
                     /translation="VLDFSKIEAGKLQFEALDFVLTNAIEETIDLLAPRAHDKKLEFV
                     SLIYKDVPVGLRGDPGRLRQVLTNLIGNAIKFTESGEVVVRAEKESEDEKHVVIRFSV
                     VDTGIGISETAQHKLFQPFTQADGSTTRKYGGTGLGLAISRQLVELMGGEIGLKSEIG
                     GGSVFWFTARFEKQTQLLNSSPLDLNVLSGLRVLIVDDNRTNRKILSHQLDSRGVKHA
                     EAESGKRALALLRSAVQTGEAYDLAILDLMMPGMDGFELARVIKSDPVIAATPLVMLT
                     SYGDRGGSATAQQLGIAAYLAKPIRQSALFECLANVVQMPHAAKVVEQTITTRAPEIE
                     VKPTSNKLILIAEDNVVNQKVAIRQLRKLGYRADAVANGREVIEALARIPYDLVLMDC
                     QMPEMDGYEATAEIRRYEANSQRTPVIAMTAHALDGDRARCIAAGMDDYVSKPVKPEE
                     LQRVLERYLAA"
BASE COUNT          827 a         1095 c         1179 g          980 t
ORIGIN      
        1 cgtaagccgt ttccccgcgg cgctgaaatc ggttgcacat tgtgatgtct gacttttcat
       61 tcggggaacc ccaacagctc aaacctcacc gggccttctc gatcgcgctg gcgtcactgg
      121 cgttttgtat cggtggcgcg gcattgatgg gctggatctt caacgtcgac gcgctcaaac
      181 gaattcatcc gtcgctcgtc aacatgaagg caaacaccgc gatttgtctc atgcttgtgg
      241 cgattgcggt ttttctaatt caggacagcc ccacatccaa agttagacgc cggctcgcgc
      301 ttttgctggc agcgattgtc gcgactgttg gagcaattac ttttagtgaa caccttttcg
      361 gctggaactc cggcctcgat cagttgttat ttcatgagtc ggtggctgaa gccggcgaat
      421 cattcccggg tcgcatggga gtcgcctcgt cgctcaacct cgtcttcttt ggtctcgcca
      481 tttcattcct tgacgcacga tcaacacgat ggtttcgcgt ttcgacgatt tcggtgctcg
      541 cgattctagc cgccactctg ctggtatttc tttactactt ctatggtatc gaacacgccg
      601 agcctctggc tcgttacttc acgatcgcga tccataccgt ggttgcgttt gtgagtttgt
      661 gcgccgccat tctgctggca cgctccgatc gggatttagt tgcgtcgttg ctgggaaata
      721 gtcctggcgc catggtggcg cgtcgtatgt tgccggcttt tctagttgtg attctgctgg
      781 gctggatcag aacgattgcc agatcaaacg gatggattag ccagggtttt gcgaccgcct
      841 cgtttgtgct ggcgatactc ttgctcctcg gcatactgat ctggctgaca gcgctgtcac
      901 tcaatcgcac cgacagggaa cgccggcgtg tcgacctcgc ccaacggcgc gcggagaatc
      961 gtctcgacgt gctgttccgt gttagcgagc tgatccgaac gctgcgcgat ccgtacgagt
     1021 tgtcatacgc ggtggccgag caagttggca tacatctcga cttgcgtcgc tgtttgttca
     1081 atgagactga catcgaccgt gatctcgaga ttgttcatcg cgattactgc gacggagttg
     1141 tctcggttgc cggcgagcat cggatttcgg attacagcag tgtgacttcg caggaaatgc
     1201 agaaggggca aacagtcgtc aacaacgatt cgaaaactga tccgcgtacc gccgccgatt
     1261 atcaacgaag ttatgaagct accggcgagc gcgcctacgt tgccatgccg ttgctgcgcg
     1321 aaggacgctg ggtggcatcg ttatgggcca gtgtcgatca gccgcgggtg tggacgaaag
     1381 aagaggtagc gctagtgcaa gccgtcgccg agcgcacctg gactgcagtt gaaaaactgc
     1441 gcgccgaagc cgaacgcgaa cgactgcttc agagcgtgca ggaagcacgc gacacggcag
     1501 agaaagcgaa tcagctcaaa gatgagtttc tggcgacgtt gtcgcatgag ctgcgcaacc
     1561 cgctgaacgt cattctcgga tactcggagc tgttattgcg tatgcgagag attgaacagt
     1621 cgccgcgact ggtgcagatg ggagaagcgt taagacgaaa cgcgctttcg cagtcgcagt
     1681 tgatcaacga tctgctcgac ttgtcgcgtc tgcaacgagg caagatttca ctgaaccagg
     1741 aaacagtttc gctgccggcg atcattgata acgccgtcga gaccgtgcgg acggacgcgg
     1801 cgaccaaggg tgtcgagatt cgtcttcagg cgagcgacca gctcttgctg gttgaaggcg
     1861 atcgactgcg actacagcag atcgcgtgga acctgctcaa taatgcggtg aagtttacgc
     1921 cggccggtgg gcgcattgag atcagtttga acaacgaagg cgatcgggcg gcgctcgtgg
     1981 ttgcggacac cgggcaggga atcgatccca gctttctgcc gcatgttttt gaaatgtttc
     2041 gtcaggcgga tggtagcaat cgccggcgtc atggtgggct cggcattggt ctcgcactcg
     2101 tgcggcaatt agttcacctg cacggcggca caatcaatgc cgagtctggt gggccaaaca
     2161 agggttcgtg tttcacggtg tgtctgccgc tgctacgcga gacagcgccg ctgagtattc
     2221 ctgcgccagt ggctacggaa ttgagtatgt tctcggggac gagttttctg gtcctggatg
     2281 attccgaaga ctcaatcatg atgctgacgg aactgctcaa gcttgccggg gcgcatgtgg
     2341 tgacggccac gaatggcgtg gatgcgttgc gaattgcgag tgagaatgag tttgatgtga
     2401 ttctttccga catttcgatg ccggagatgg atggatttga gtttctaaga cgactacgaa
     2461 agatcgatgg gcggcaggat gtaccagttg ttgcgatcac cggcttcggc cgcagtgacg
     2521 acatcaaccg cgcgcgtgac gccggatttt attcgcacat cacgaagcca ttgaacttgc
     2581 aggcgcttaa cactgtgctt gaacagatcc tgaatggcca caaaaggcag tccacagatt
     2641 acgcagatgg aaaaaaacag aaatctgcgt aatctgtgga ccgccttttg gaggttacgc
     2701 ggcgaggtag cgttcgagta cgcgttggag ttcttccggt ttcaccggct tggatacgta
     2761 atcgtccatg ccggccgcga tacaacgggc acggtcgcca tcgagcgcgt gtgccgtcat
     2821 cgcgatcacc ggagttcgct gtgaattagc ttcgtatcgc cgaatctccg cagtggcttc
     2881 gtacccgtcc atctcgggca tctgacagtc catcaacacg agatcgtaag gaatgcgcgc
     2941 gagtgcttcg atcacttcgc ggccattagc gaccgcgtcg gcgcgatagc ccagcttccg
     3001 cagttgccgg atcgcgacct tttgattaac cacattgtct tcggcaatga gaatgagttt
     3061 gttcgacgtt ggtttgactt caatctccgg tgctctcgtc gtaatcgttt gctcgacaac
     3121 tttcgcagcg tgcggcattt gcaccacgtt cgcaagacac tcgaacaaag cagactgccg
     3181 gatcggtttt gccagataag cagcgattcc gagttgttgg gccgtagccg agccaccacg
     3241 atcgccatac gacgtgagca tcacgagcgg cgttgctgcg attaccggat cgctcttgat
     3301 cacgcgggcc agttcgaaac catccattcc cggcatcatc agatccagaa tcgcgaggtc
     3361 gtaagcttca ccggtttgca cagccgatcg cagtaatgcg agtgcgcgct tccctgattc
     3421 ggcttccgcg tgtttcacgc cgcgtgaatc gagttgatgc gacaggatct tgcgatttgt
     3481 tcggttgtcg tcgacgatca aaacgcgcag tcctgacagc acgttgaggt cgagtggcga
     3541 tgaattgagt aactgtgttt gtttctcgaa tctggccgta aaccagaaga cggaacctcc
     3601 gccgatctcg ctcttcaatc cgatttcacc gcccatcaac tcgactagct gtctcgaaat
     3661 cgcgaggccc aatccggtgc cgccgtattt tcgcgtagtt gagccgtcgg cttgcgtgaa
     3721 tggttggaag agtttgtgtt gagccgtttc gctgatgccg atgccagtgt cgacaacgct
     3781 gaaacgaatg acgacatgct tctcgtcttc gctttctttc tcagcgcgga cgacgacttc
     3841 gcccgactcg gtaaacttga tcgcattgcc gatcagattg gtgagcactt gtcgcagccg
     3901 gcctggatcg ccgcgcaatc cgacgggcac gtccttataa atgagcgaca caaactccag
     3961 cttcttgtcg tgcgcgcgag gcgccagcag atcgatcgtt tcctcaatcg catttgtgag
     4021 cacaaaatcg agcgcttcga attgcagctt gccggcttcg atcttcgaga agtcgagcac
     4081 g
//
LOCUS       DASSFQ010000058         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1513498, whole genome shotgun sequence.
ACCESSION   DASSFQ010000058 DASSFQ010000000
VERSION     DASSFQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513498"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..854)
                     /locus_tag="VFH96_01775"
     CDS             complement(228..854)
                     /locus_tag="VFH96_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6972720.1"
                     /translation="MRFAENTLKKLSSSFIGVQVDPINYMQATDKELLLSCRQGNELS
                     WQALVDRYQRLVYAVPRRAGLNEDQSGEVFQEVFVTLFEKMNEINEPDRLHAWLVTTA
                     RRKTWRLLSKERSRQSVQTGEDDEGLVVVDDSPLPDETLVRLEEQHRIRTALASLDDR
                     CQQLLTMLYYEAEPPPYSEIAAMLGMPEGSIGPTRARCLKKLLKLLGK"
     gene            948..2168
                     /locus_tag="VFH96_01780"
     CDS             948..2168
                     /locus_tag="VFH96_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6972721.1"
                     /translation="MKQATALIHPAVRPVKPARLTNPRILLYSHDTFGLGNIRRTLLL
                     AQEVIEQYPKAAVLLVTGSQMIHSFRIPEGVDYIKLPCLDRIDAERYESRFLLECSEA
                     VKRTRRAIIERTVLGFAPDLMIVDKRPAGVDGELTETLTALRHSRSPTRLVLGVRDIL
                     DEPRKTRETLRNNHSFEIIRDYYDEVWIYGSQTIFDTVSAYDFPEDVARITHYCGYLK
                     RPTVAPSRNDGPPHVLVTTGDGGDGSHMIETYLEGLSNLPRNVALKSTVVFGPQMSAD
                     KRATLINSFGYLTDVQFREFEADLTELYAEADLVVSMAGYNTVCELLSFGRNAILVPR
                     AQPVKEQLIRARLLAAAGLFEMVEPDDLSPDLLMTKVLASLKHASEQPVRVDLQGLPR
                     IRERIRDLLEEKER"
     gene            2165..3232
                     /locus_tag="VFH96_01785"
     CDS             2165..3232
                     /locus_tag="VFH96_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6972722.1"
                     /translation="MKLAVIVSGFPRRSETFMLGELLALEANGVLAGVFATKLGETGP
                     AQPGTERLTTYVEFLPDVSIEEQARAVRMMLARKRVDGIHAYFAHRPAEVAQQAARML
                     NVPFGFSTHARDSRKVAPEVLSQRARDAACVVACNTDVARDIRALGSEVQLLPHGVDL
                     QRFTPCAASANGVLRLLAVGRLVEKKGFHFLIAALAGVKIPFTLTIAGDGPERERLQQ
                     LVSLNNLENRVVFCGSVTHSELPRMYTDADVVVVPSVQDSSGDRDGLPNVVLEAMASG
                     RPIVASDAGAIASAIVDHENGLLVPPADPVALGQAIQRLAESRKLREQLGRNGRRRAE
                     IDYEVGQCSERFHRLLTTVYA"
     gene            3311..4630
                     /locus_tag="VFH96_01790"
     CDS             3311..4630
                     /locus_tag="VFH96_01790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6972723.1"
                     /translation="MIPETQGAVAYVLKGYPRLSEIFITSEIYRLEQLGVKLRLAVIK
                     HGDELHTNSLFDKIQARPNYLPQTSSLSQTSLRRWLSLHLKDFLPTVFNVFKRRPSGF
                     ARAAIAASAQAVRARKSFLAWPRKLYLKEFLQAVWTTRLILDAGDVSHIHAHFCHGST
                     TVAWLASIITGLPFSFTAHAKDIYCESLNPAGLLRRKMRAAQFVVTCTDANREYLLNI
                     EPNANVRCIYHGLNAEFADFLLDASNAPIKTDRLRALGVGRLVPKKGFDVLVEACAIL
                     KRRGFHFETAIVGEHGEHEPQLRRQIEAHGLKDIVRLVGPMEQSRLYTEYQRADIFCL
                     PCRVLDNGDRDGLPNVLMEAMACGLPVITTPVSGIPEIIKDGQNGALVPPEDAEALAN
                     AIQRISADRMLARNLGRAGRLTVLEHFDGDKTAMELAALFATATEGV"
BASE COUNT         1021 a         1346 c         1337 g         1070 t
ORIGIN      
        1 agatctcata aatccaatcg tctgcctgat cgcctgcaac tggctcgaac actcagaaca
       61 cactgcaagg tgagccatcg cttccggcga aggctgctct tccgccagcg cggccaactc
      121 tgaaaatggg atgtgcggtg agaagatgtt cataatttgg tcatccttaa gaggtggggt
      181 tcatctaccc gaccaaacag tgcctcaccg cacgaataaa atacacgtca tttccccagc
      241 aatttcagca atttcttcag acatcgcgcg cgcgtcggtc caatgcttcc ttccggcata
      301 cccagcatcg ccgcgatctc cgaatatggc ggcggctcgg cctcataata gagcattgtt
      361 aggagttgct ggcagcggtc gtcgagacta gcgagcgcgg tacggatccg gtgttgctcc
      421 tcgagacgca cgagtgtttc atcaggcagc ggcgaatcgt caaccaccac gagtccctcg
      481 tcatcttcgc cggtctgtac tgattgacga gatctctctt tgctcagcaa gcgccacgtc
      541 ttgcgtcgcg cagtcgtcac cagccacgcg tgcaggcgat cgggctcgtt gatctcattc
      601 atcttctcaa agagcgtgac aaaaacctcc tgaaaaacct cgcccgactg gtcctcattg
      661 agacccgcgc gccttggaac ggcataaacc aaacgctggt aacggtcgac gagcgcttgc
      721 caagacagct cattcccctg ccggcacgag agcagcagtt ccttgtcagt agcttgcatg
      781 tagttgatcg gatcgacctg aacgccaata aaacttgagg ataatttttt aagtgtattt
      841 tccgcaaatc tcatcgcact gtttcctcgc aacgagtcag tttatgactt gtctcccccg
      901 gcaaatcccg agactccaac acttcggttc tgaggcacat gctccagttg aaacaagcta
      961 ctgcgttaat tcatccggcc gttagacccg tcaagccagc tcgcttaacg aatccgagaa
     1021 ttcttctcta ttcgcatgac acgtttggac tcggcaatat ccgccgcacg ttgctgctgg
     1081 ctcaggaagt catcgagcaa tatccgaaag cggccgtgtt gctggtaacc ggctcgcaga
     1141 tgattcattc gtttcggatt cccgaagggg tcgactacat caagctgccg tgtctcgatc
     1201 gaattgatgc ggaacgatac gaatcacgtt ttctgctcga atgctcggaa gcggtcaaac
     1261 gcacccggcg tgccatcatc gaacgaactg tgttgggatt tgctcctgat ttgatgatcg
     1321 tcgataagcg accagcggga gtcgatggcg agttaactga aacgctcacg gccttacgac
     1381 acagccggtc tcccacgcgc ctggtcctgg gcgtacgcga tatcctcgac gagccacgca
     1441 aaactcgcga gacactcaga aacaatcaca gcttcgaaat cattcgcgac tactacgacg
     1501 aggtctggat ctacggctcc cagacgatct tcgacactgt cagtgcgtac gactttcccg
     1561 aagacgttgc gcgcattact cactactgcg gttatctgaa gcgaccgaca gtggctcctt
     1621 ctcgcaatga tggaccaccg cacgtcctcg tcacgaccgg agacggcggc gacggtagcc
     1681 acatgatcga aacctacctc gaaggccttt cgaacctgcc gcggaacgtg gcgttgaaat
     1741 cgacggtcgt gtttggaccg cagatgtctg ccgacaaacg cgcgacgctc attaacagct
     1801 ttggctactt gacggacgtg cagtttcgcg aattcgaggc tgacctgacg gagctctatg
     1861 ccgaagcgga tctggtcgtc tcgatggcgg gctacaacac cgtttgcgag ctgctgtcgt
     1921 ttggacgtaa cgcgattctt gttccgcgcg cacagccggt taaggaacaa ctgatccgcg
     1981 cgcgtttgtt agctgctgcc ggactgtttg agatggttga gccggacgat ctctcgcccg
     2041 atttgctgat gacgaaagtg ctggcgtcgt tgaagcatgc gtccgaacag ccggttcgtg
     2101 ttgatttgca gggtttgccg cgcattcgcg agcgcatccg cgatctcctg gaggagaagg
     2161 agcgatgaag cttgccgtca ttgtgagcgg ctttccgcga cgttccgaga cgttcatgct
     2221 tggcgagctg ctcgcgctcg aggcgaacgg cgtattggcg ggtgtgtttg ccacgaagct
     2281 cggcgagacg ggtccggcgc aaccgggcac tgagcggctg acgacgtacg tcgagttcct
     2341 gcccgatgtg tcgatcgaag aacaggcgcg ggccgtgcgc atgatgctgg ctcgcaaaag
     2401 ggtcgacggt attcatgcat atttcgcgca ccggccggct gaagtcgcgc agcaggcggc
     2461 gcgaatgttg aacgtgccat ttggctttag cacacatgcg cgtgactctc ggaaagtcgc
     2521 gcctgaagtt ttgagccaac gggcgagaga cgctgcctgc gttgtcgctt gtaataccga
     2581 tgtcgcgcgt gacattcgcg cacttggatc agaagttcag ctactgccgc atggcgtcga
     2641 cttgcagcgg ttcacaccat gcgctgcaag tgcaaatggc gtgttgcgct tgctggcggt
     2701 gggccggctg gttgaaaaga aggggttcca ctttctgatt gcggctcttg ccggggtgaa
     2761 gattcctttc acgctcacga ttgccggcga cggacctgaa cgagaacgcc tgcaacaact
     2821 cgtcagtctt aacaacctgg aaaatcgcgt cgtcttctgt gggtcggtta cgcattcaga
     2881 gctgccccgg atgtataccg acgccgacgt tgtagtcgta ccgtcggttc aggatagctc
     2941 gggtgatcgt gatggtttgc caaacgtcgt gcttgaagcg atggcgtcgg gtcgtccgat
     3001 cgtggccagc gatgcgggcg cgatcgcgag tgcgatcgtc gatcacgaga acgggttgct
     3061 cgtgccgccg gcggatcccg tcgccctggg ccaggcaatc cagcggctcg cggaaagcag
     3121 aaaacttcgc gaacaactcg gccgcaacgg aagacgccgg gctgagattg attacgaggt
     3181 gggccagtgt agtgagcgtt ttcacaggct gctgacgact gtctatgcgt aaacaggtag
     3241 ccacaaaaag gcacaaaaag cacaaaaact gactttgtgt cttttgtgcc tctttgcggc
     3301 aaatttaaaa atgatcccgg aaactcaagg cgcagtcgca tatgttctta agggctatcc
     3361 gcgcctttcg gagatcttta tcaccagcga gatatatcgt ctcgagcagc ttggtgtgaa
     3421 gctgcggctc gcagtgatca agcacggcga tgagctgcat acgaatagtc tcttcgacaa
     3481 aatacaggct cgtccaaact acttgccgca aacttcatcg ctgtcgcaaa catcgctgcg
     3541 acgctggctc agtctgcacc tcaaagattt tctgccgacg gttttcaacg ttttcaagcg
     3601 acgaccatca ggctttgctc gcgctgcgat tgctgcgtct gcacaagccg taagagcgcg
     3661 caagagtttc ctcgcgtggc cgcgtaagtt gtacctgaaa gaatttctcc aggcagtttg
     3721 gaccacacga ctgattcttg atgcgggtga tgtcagtcac atccatgcgc acttctgtca
     3781 tggctcgacc acggttgcat ggctcgcgtc gatcatcacc ggcttaccgt tctcgttcac
     3841 ggctcatgca aaagacatct attgcgagtc gctgaacccg gccggattgt tgcgacgaaa
     3901 gatgcgcgcg gcccagtttg tcgttacctg cactgacgcc aaccgtgaat acctgctcaa
     3961 cattgaaccc aatgcgaacg ttcgttgcat ctaccacggt ctgaacgcgg aattcgctga
     4021 cttcctgctg gatgcttcga atgcacccat aaaaacggac cgcctgcgcg cactcggtgt
     4081 cgggcggctt gttccgaaga aaggcttcga cgtgctggtt gaggcttgcg cgatcctgaa
     4141 gcgtcgcggc tttcatttcg agacagcgat cgtaggcgag catggcgaac acgaacctca
     4201 actccgccgg cagattgaag cgcacggact gaaagacatc gtacgcctcg ttggaccaat
     4261 ggagcagtcg cgcctctaca ctgaatacca gcgcgccgac attttctgcc tgccttgtcg
     4321 cgtgctcgac aacggcgatc gcgacggtct gccaaacgtg ttgatggagg cgatggcgtg
     4381 cggtttacct gtaatcacta cgcctgtttc cggcattccg gagattatca aagacggtca
     4441 gaacggcgcc ctcgttccgc cggaggatgc ggaagcgctt gcaaatgcga ttcagcgaat
     4501 cagcgccgat cgcatgctgg ccaggaatct cggtcgcgcc ggacgattga ccgtgctcga
     4561 acatttcgac ggcgacaaga cagcgatgga gctggctgca ctgtttgcta cggcaacgga
     4621 aggagtgtga agggtgcaag ttgcctttga acaaacgact tactcctttc ccacgatgac
     4681 caacaggtgg cgcgatgttt tctgcatcat cgagaacgcg tatcgcgaca tctcgatcgc
     4741 ggacaacgta tgtgatggcg tcttcacctt tgcg
//
LOCUS       DASSFQ010000059         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1812657, whole genome shotgun sequence.
ACCESSION   DASSFQ010000059 DASSFQ010000000
VERSION     DASSFQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812657"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..672)
                     /locus_tag="VFH96_01795"
     CDS             complement(79..672)
                     /locus_tag="VFH96_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972724.1"
                     /translation="MKSAAKKTPARRTEDQRWTMDIATLRQAAQELVQRRSEAPKSPT
                     NTQFDQIAELLDDPSAAVREKAVRDLYTLDPDRAATLVNDALRDGSPDERRRIGNALA
                     DSGLLYEAIDDLMAENHEHCYSAFSLLFLVAKAGVVEPLSEVIEKHPSLDLSLAVIRL
                     LASSGEPEVTTTLERLAANTALAPEIRSAAAESISHF"
     gene            complement(669..2135)
                     /locus_tag="VFH96_01800"
     CDS             complement(669..2135)
                     /locus_tag="VFH96_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972725.1"
                     /translation="MLFLESRTLNHQLDTAGRLGLLQELQARSVVPLIRSHRLRTFAD
                     VVLDADRIADMEAALAASTESTTGEPYVRGDLFSFSDYILFLVFEDDDDLIRAGIVYE
                     ANTPEPFRKLDSFCQDIRSFLNQQNAGWYPEWQTTSPPMPQGFRTFVERQDADSLYTS
                     LRKESMSKRILAASKLEDETARVFLRTARNAHQEGYAAKLLTGHSESRDVPIQRLEDA
                     GLVEREVQISCRKTGHALFRLPNPHALAVVTVSDATCSECGAPVADENVEEVIAPTQL
                     ASSLLEDGSWLVSRIHFLLREMGVPEREIALGPSEGTGYGQLMANICGESFLIVTRDG
                     DLTPAFARWTIDHEIDTEASHLVIVATGRIHKEAGVLLQNHSRRRISSGQDFEMIVAD
                     DAASAGRELEQALERVSQRVVAEQLCVLDGNFGFSVSRLVLTKFQLQRAAAPKEAEVV
                     TESEPEFVASAPLTLAAHASGGDVYEAPRDQEQPETLQ"
     gene            complement(2243..>2903)
                     /locus_tag="VFH96_01805"
     CDS             complement(2243..>2903)
                     /locus_tag="VFH96_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972726.1"
                     /translation="RSIEPSLQRAVSELFGCWDESAVQIEGKAEPVQLEGEAHSRNLV
                     GGRRSELLALPPGPSLLVQRRSWILAAAAIVVVVGLSSVIAMTGGFKNNQPETSLIQQ
                     PQAPVAPAAAPAESPKSQATLAAAVVKPKKQIKPEEKSTVEPAAETKPAETVKEPESS
                     SQPIRVVMQIENGRVLKASIANPKPGMDSYEALALRIARQRRYPATMNGGETITINVN
                     R"
BASE COUNT          586 a          854 c          803 g          660 t
ORIGIN      
        1 ggctgatccc cttttggttt gtattcagaa tgaagtttga aacgcactca tacatcttgg
       61 tcaatcttgt aaatcctgtc aaaaatgact gatggattca gcggcggctg agcgtatctc
      121 gggagcgaga gcagtgtttg cggcaagacg ctctaatgtt gtagtgactt caggttcacc
      181 acttgaggcg agcaatcgga taacggcgag actcaaatca aggctgggat gtttctcgat
      241 cacttcgctc agcggctcga ccacgcccgc ttttgcaacc agaaacaaaa gagaaaacgc
      301 gctgtaacag tgttcgtgat tttcagccat cagatcgtcg atggcttcgt aaagcagacc
      361 cgaatccgcg agggcattgc cgatgcgacg gcgctcgtcg ggtgatccgt cgcgcagggc
      421 atcgttcacg agcgtcgcag cgcgatcggg atcgagtgtg tacagatctc tgactgcctt
      481 ctcgcgcact gccgccgagg gatcgtcgag caattcggct atctgatcga attgagtatt
      541 agtgggcgat ttcggagctt cactgcgacg ctgcaccagt tcctgcgcgg cctgtcgcag
      601 agtagcaatg tccatggtcc agcgctggtc ctccgtgcgt cgcgccggcg ttttcttcgc
      661 ggcgctcttc attgcagcgt ttctggctgc tcctgatcgc gcggggcctc gtaaacgtca
      721 ccacccgacg cgtgggcggc aagcgtcagc ggagcgctcg ccacaaactc aggctcactt
      781 tctgtcacaa cttcagcttc cttcggcgct gccgcgcgct gcagttgaaa cttcgtcaac
      841 actaaacgac tgaccgagaa accgaagttg ccatccagca cgcagagctg ttccgccact
      901 acacgctgcg aaactctttc gagcgcttgt tccagctcgc gtccagccga cgcggcatcg
      961 tcggcgacaa tcatttcgaa atcctgccca ctgctgatgc gacgtcgcga gtggttctgc
     1021 agtaacactc cggcttcctt gtgaatccgg ccggtcgcga caatgacgag atgcgacgct
     1081 tccgtgtcga tctcgtgatc gatcgtccag cgcgcgaatg ctggagtcag gtcgccgtca
     1141 cgcgtcacga ttaagaatga ctcgccgcag atgttcgcca tcaactggcc gtaacccgtt
     1201 ccttcagatg gccctagcgc aatttctctt tccgggacac ccatttcgcg caacagaaaa
     1261 tgaatacgac taactaacca cgaaccatct tcaagcagcg acgacgcaag ctgtgtcggc
     1321 gcaatcactt cttccacgtt ttcatcagcc accggcgcgc cacactcgct gcaagtcgca
     1381 tcgctgacag tcaccacggc gagcgcgtgc gggttcggca gacgaaacag cgcgtggcca
     1441 gtcttgcgac aactgatctg cacttctcgc tcgaccagtc cggcgtcttc gagtcgctga
     1501 atcgggacat cgcgcgattc gctgtgaccg gtgagcagtt tggccgcgta accttcctga
     1561 tgcgcgtttc gtgccgtacg taaaaacacg cgcgcggttt cgtcttcgag tttcgaggcg
     1621 gcgagaatcc gtttgctcat cgattcttta cgcaggctgg tgtagagcga gtcggcgtcc
     1681 tgtctctcga cgaacgttcg aaatccctgc ggcatgggcg gactggttgt ctgccattcc
     1741 gggtaccaac cggcattctg ctgattaaga aagctgcgaa tgtcctggca gaaagagtcg
     1801 agtttgcgaa atggttcggg agtatttgct tcgtaaacga tgccggcgcg aatcaaatcg
     1861 tcgtcgtctt cgaagacgag gaagagaatg taatcgctga atgagaacag atcgccacgg
     1921 acgtaaggtt cgccggttgt cgactcggtc gatgcagcga gtgcggcttc catgtcggcg
     1981 atgcggtccg cgtcgagcac aacgtctgca aaggtgcgca gccggtgcga gcggatcagc
     2041 ggcacaacgc ttcgtgcttg cagttcctgg agcaacccaa gccgtccggc ggtgtcgagt
     2101 tgatgattga gcgtcctgct ttcgagaaat aacatcggga gtacctctgt tcaatcaaga
     2161 gtttcacgca aaggcgcaaa gacacaaagc aagaacactg cttcaacttt gctgctttgc
     2221 gcctttgcgg gaaaacaact tcttaacgat tgacattgat cgtgatcgtc tctccgccat
     2281 tcatcgtcgc tgggtaacga cgttgccggg cgatgcgcag tgcgagcgct tcgtaactat
     2341 ccatgcctgg tttgggattc gcaatcgagg ctttcaacac gcgcccattc tcgatctgca
     2401 tcacgactct gatcggttgc gagctggact ccggctcctt cacagtttct gcgggcttgg
     2461 tttcagccgc cggttccacg gtgctttttt cttccggctt gatttgcttc tttggcttta
     2521 caacagccgc cgcaagcgtc gcctggctct tcggcgactc agccggagcg gccgcgggcg
     2581 caacaggcgc ttgcggttgc tgtatcaagc tggtttcggg ttggttgttt ttgaatccgc
     2641 ccgtcattgc gataacgctc gaaagtccaa ccaccaccac gatcgccgcc gcagccaaaa
     2701 tccacgagcg acgctgaacc aacaacgatg gtccaggcgg gagcgccagc agttccgaac
     2761 gccgtcctcc caccagattc cgggaatgcg cttcaccttc gagttgcact ggttcggcct
     2821 tcccttcgat ctgcaccgcg ctctcgtccc aacaaccaaa cagttccgag accgcgcgtt
     2881 gcagcgaagg ctcgatcgat cga
//
LOCUS       DASSFQ010000060         6071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_769333, whole genome shotgun sequence.
ACCESSION   DASSFQ010000060 DASSFQ010000000
VERSION     DASSFQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769333"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VFH96_01810"
     CDS             <1..1408
                     /locus_tag="VFH96_01810"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET6972727.1"
                     /translation="VGPDVQDVYVEKFNPDNPKQYQTPSGWQDVVIRHEQIKVRKAFG
                     SSEYDTVTYDVTVTRHGPIIFEGDGKRYALRWTALDPTKNNPESTFVVNRARNWKEFN
                     TALESFTAPTQNIVYADVDGHIGYHAAGVVPIRKSGDGSVPYDGSTDAGEWTSYIPVS
                     KLPTLYDPPSGIIVTANQRIVGTDYPYFLTHSWAQPYRARRIFDLLSEKPKLSSDDFR
                     RILGDVYSIAGVSFAKEAVKLLRPKLTPADEKLKAHLDTFEKWDGRVNPESNVVPLLA
                     YLRIAFRSKILTAALGQELVKNYQWNNFDTTLDRVIKEQPASWLPKEYASYGDLLRAS
                     YDEAIAMLTKNLGADETKWTWGELAKARFPHPLGGAPLIGGQFTVPPFPQNGTGGFIG
                     ATVNVGQAVSMRLIADPSDWDKTQHGIALGESGLPKNPHWSDQLADWRAVTPREFPFT
                     AEAVLKATKETLVLEPAK"
     gene            1405..2004
                     /gene="thpR"
                     /locus_tag="VFH96_01815"
     CDS             1405..2004
                     /gene="thpR"
                     /locus_tag="VFH96_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET6972728.1"
                     /translation="MSHKEAQKAQKDWRVFCAVELPAEVRARLEEHILRLRREVPDAA
                     ASWSRVENIHLTLKFFGNVEVKRIEKISAAAERVAKQFSTFQIGVGETGVFPRPSRAQ
                     VLWIGISDPSGQLSALQERLENECAAEGFAKEDRAYHPHLTIARLRKPEGARHLADAH
                     LQMRFETIEVELKEIVVFRSELSPKGSKYTAISAADLRG"
     gene            complement(2013..3029)
                     /locus_tag="VFH96_01820"
     CDS             complement(2013..3029)
                     /locus_tag="VFH96_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET6972729.1"
                     /translation="MDAVGALLAFMFFGFIAIVLLVIGVKTIRIVPQATVMLIERLGS
                     FHHLAQNGLNILWPFIDRPRAVYWTNTRPGLTSIDLREQYIDLPPQPVITRDNVTIHV
                     DSVVYWQVTDPIKAVYEMNDLVGGIVQLTITGMRAVMGDMDLDHTLSQRDQINSKLRL
                     ILDEATDKWGVKVTRVDVKNINPPEDVRITMEKQMTAERNRRALVLQAEGDRQAAITR
                     AEGEKQAAVTRAEGEKQSAILAAEGAAQARLRNAEAESEALTRIAQSIPSGDPGHYLI
                     TMRYIESLRDMTRSNNSKVIFMPVEASSMLSSVGAIKEVFSQSGEKDDQTPPPPPRPR
                     ELPR"
     gene            complement(3071..4456)
                     /locus_tag="VFH96_01825"
     CDS             complement(3071..4456)
                     /locus_tag="VFH96_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6972730.1"
                     /translation="MKNADLIKETLGTIAAMIRKREVSPVEVVEAHLERIAELNPELN
                     AIVTLAPDAIERAREAEIAVMRGAGNGVLQGVPVTIKDTIETAGLRTTSGSKMRMDYV
                     PQTDAPAVARLKAAGAIVLGKSNAAEMAMDYTADNPVFGRTNHPLNHDLTPGGSSGGE
                     AVAIATFMSPGGLGSDLAGSVRIPAHFCGVCALKPTTGRVPGELQFPPSIGPYSLGAV
                     IGPLARTAADLRLLFAALSKDPFERKSHDLRGVRVAWYTDDGVAPVTDETARAVAAAA
                     RALSDAGLKVEEQRPPHVERGNELWLKVFSRASVVQLRGLYKGRENEGGSFVSWRLGT
                     ADHTPAPALDEYIAGWMERDRLREELVQWMETTPIIIAPVGATPAYPHDTLKVTVRDN
                     TMGTFKAFSYAQTFSVFNLPVVTIPAGKSNDGLPIGVQIAGGPFTEELILAAAEIVET
                     ALSTDYTDSKL"
     gene            4565..5239
                     /locus_tag="VFH96_01830"
     CDS             4565..5239
                     /locus_tag="VFH96_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HET6972731.1"
                     /translation="MAIRLSRTFVLRKLHQLSGIVPLGLFLLEHFYTNSKALTGANDF
                     NKSVVDLQSIPYILFVEIGGIFIPLIYHAVYGLVITMEARPNNLAYPYSRNWFYLIQR
                     ITGVILFFFITFHVLNFRFGMIPGLNTESVAHHADRAFEIVAREFRMIPIFLVYMIGI
                     TATVWHLANGIWLFLVDWGITIGERAQRLTGYACIAFGVVLLLVGINAAVAFIRPGGL
                     LGGIIY"
     gene            5239..>6071
                     /locus_tag="VFH96_01835"
     CDS             5239..>6071
                     /locus_tag="VFH96_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6972732.1"
                     /translation="MASNGLKIAIVGGGLAGLAAAMKIAEAGHAVDLFSVVPVKRSHS
                     VCAQGGINGAVNTKGEGDSTWEHFDDTVYGGDFLTNQPPAKAMCEMAPAIIYLFDRMG
                     VPFSRTPEGLLDFRRFGGTKHHRTAFAGASTGQQLLYALDEQVRRYEVNGKVRKFEHW
                     EMMSLVLDEHQVCRGLIAMDLQTLELKAFPADAVIMATGGPGLIFGKSTNSMVCTGSA
                     VSACYQQGARYANGEFIQVHPTSIPGEDKLRLMSESARGEGGRVWVPRKEGDVRPPSS
                     IP"
BASE COUNT         1296 a         1708 c         1686 g         1381 t
ORIGIN      
        1 tgtcggtcct gatgttcagg atgtgtacgt tgagaagttt aatcctgaca atccgaagca
       61 gtaccaaacg ccgagcggtt ggcaggacgt ggttatccgt cacgaacaga tcaaggtcag
      121 gaaagccttc ggcagctctg aatacgacac cgtgacgtac gacgtgaccg ttaccaggca
      181 tggtccaatc atcttcgaag gcgacggaaa gcgttatgcg ctgcgctgga cggcgctcga
      241 cccgactaag aacaaccccg agagtacttt cgtcgtcaat cgcgcgcgta actggaaaga
      301 gttcaataca gcgctcgagt cgtttaccgc gccgacgcag aacattgttt atgctgacgt
      361 cgatgggcac atcggttatc acgccgcggg tgtggtgcca atcaggaagt cgggtgacgg
      421 cagtgtgccg tacgacggat cgactgacgc aggcgagtgg accagttaca ttcccgtatc
      481 aaagctgccg acgctttacg atccaccatc gggaatcatc gtcactgcca atcagcgcat
      541 cgtcggcact gactatcctt atttcctaac gcatagttgg gcgcagccgt atcgcgcgcg
      601 ccggatattc gatctgctca gtgaaaaacc aaaactttcg tctgacgatt ttcgtcgtat
      661 cctgggagac gtttactcga tcgcgggcgt gtcgtttgct aaagaagcag tcaagttact
      721 gcggccaaag ttgacgcccg cagacgagaa gctgaaggcg catctcgata cgttcgaaaa
      781 gtgggacgga cgagttaacc ccgaatccaa tgtggtgcca ctcctggctt atctgcgtat
      841 cgcctttcgc tcgaagattc tgaccgcggc gctgggccag gagctggtga aaaactatca
      901 gtggaacaac tttgatacga cgctcgatcg tgtaatcaag gagcaaccgg cctcctggct
      961 gccgaaagaa tatgcaagtt acggcgattt gctgcgcgcg tcttacgacg aagctatagc
     1021 catgctcaca aaaaatctag gcgctgacga aactaagtgg acctggggtg agttggcgaa
     1081 agcgcgtttt ccacatccac tcggtggcgc gccgttgatc ggcgggcaat tcacggtgcc
     1141 gccgtttcca caaaatggga ccggtggttt catcggcgct acggtgaatg tcggacaggc
     1201 ggtgtcgatg cgcttgatcg cggatccgag cgattgggac aagacgcaac atgggatcgc
     1261 gctcggcgaa tcaggattgc cgaagaatcc gcattggagc gatcagcttg ctgactggcg
     1321 cgccgtaacg ccccgcgagt ttccgtttac ggcagaggct gtcttgaaag caacgaagga
     1381 gacgctcgtc cttgagccag cgaaatgagc cacaaagagg cacaaaaggc acaaaaagat
     1441 tggcgtgtct tctgtgccgt tgagcttccg gctgaggtcc gtgcgcggtt ggaagagcat
     1501 attttgagat tgcgcagaga agttccggat gcggcggcga gttggagtcg ggtggagaac
     1561 attcatctca cgttgaagtt ctttgggaac gttgaggtga agcggattga gaagatctcc
     1621 gcggctgcag agcgggtagc gaaacagttc tcgacgtttc agatcggtgt tggtgaaacg
     1681 ggtgtgtttc cgcggccgag tcgtgcgcag gtgctttgga ttggtatcag cgatccgtcg
     1741 ggccagctga gtgctttgca ggagagattg gaaaacgaat gcgccgcaga aggattcgca
     1801 aaagaggatc gcgcttatca tccacaccta acgatcgcac gactccgcaa accggaaggc
     1861 gcacgacatc tcgccgatgc tcatttgcag atgcggttcg aaacaatcga ggtggagctg
     1921 aaagagatcg ttgtctttcg gagtgagctc agccctaagg gctcgaaata cacagctatt
     1981 tctgccgcgg atctgcgcgg ataaacgcgg atttaccgcg ggagctcgcg cgggcgcggt
     2041 ggtggtggcg gcgtttgatc gtccttctcc ccgctctgcg aaaacacttc cttgattgcg
     2101 cctacactcg aaagcatcga cgaagcttca accggcatga agatcacttt cgaattatta
     2161 ctccgcgtca tgtcgcgcag gctctcgatg taacgcatcg tgatcagata gtggccagga
     2221 tcgccgctgg gaatcgactg agcgattcgc gtcaacgctt ccgactcagc ttcagcatta
     2281 cgcagacgcg cctgcgccgc accttcagcc gcgaggatgg ccgactgctt ttcaccttcc
     2341 gcacgtgtca ccgcagcctg cttctcacct tccgcgcgcg tgattgccgc ttgtcgatcg
     2401 ccctcggcct gcaacacgag cgcgcgccgg ttgcgttccg cagtcatctg cttctccatc
     2461 gtgatacgca catcttccgg cggattgatg ttcttcacgt cgacgcgtgt aaccttcacg
     2521 ccccatttgt cagtcgcttc atcgagtatc agacgcaact tcgagttgat ctgatcgcgt
     2581 tgtgaaagcg tgtggtccag gtccatgtca cccatcaccg cgcgcatgcc ggtaatggtc
     2641 aactgcacga taccgccaac cagatcgttc atttcgtaga cggcttttat cggatcggtg
     2701 acctgccagt acacgactga atcaacatga atcgtgacgt tgtcgcgagt gatgactggc
     2761 tgtggcggaa gatcgatgta ttgctcgcgt aagtcgatcg acgtcaggcc gggacgtgtg
     2821 ttggtccagt aaacagcgcg cggccggtcg ataaatggcc aaagaatgtt gaggccattt
     2881 tgcgcgaggt gatgaaagct gccgaggcgc tcaatcagca tgacggtggc ctgcggcacg
     2941 attctgatcg ttttcacgcc gattacgagc aaaacgatcg cgatgaaccc gaaaaacatg
     3001 aaggccagaa gtgctcctac tgcatccatt agtcaagact cctatccaca gattccacag
     3061 attgcgcaga ttatagcttg gaatctgtgt aatctgtgga taaagctgtt tctacgattt
     3121 cagcagcggc gagaattagt tcctcagtaa atggaccacc ggcaatctgc acaccgattg
     3181 gtagcccatc attcgatttc ccggccggaa tggttacgac aggcaggttg aaaacactga
     3241 acgtttgcgc gtagctgaac gctttgaatg ttcccatcgt gttatcgcga acggtgactt
     3301 tcagcgtgtc gtgtggataa gccggcgtgg cgccgactgg cgcgatgatg atcggcgtcg
     3361 tctccatcca ttggaccagc tcttcgcgaa gccgatcgcg ctccatccaa cccgcgatgt
     3421 attcatccaa cgcgggcgca ggtgtgtggt ccgcggtgcc gagccgccaa ctaacgaatg
     3481 atccaccctc gttctcacga cctttataca gtccgcggag ctgtacgaca gacgcgcgcg
     3541 aaaacacttt caaccagagc tcgttgccgc gctcgacatg cggcggccgc tgttcttcaa
     3601 ctttcaaacc cgcatcgctc aacgcccgcg ccgctgccgc aaccgcacga gccgtctcgt
     3661 cagttacagg cgcgacaccg tcatccgtat accacgcaac acgaacgcct ctcagatcat
     3721 gcgattttct ttcaaatgga tctttgctga gtgcggcgaa cagtagtcgc aagtcagccg
     3781 ccgtacgggc cagtggtcca atcacagcgc cgagcgagta ggggccaata ctcgggggaa
     3841 actgcaactc acccggaaca cgcccggtcg tcggcttcaa agcacaaacg ccacagaaat
     3901 gcgccgggat tcgcaccgat cctgcgagat cgctcccgag tccgccgggc gacatgaacg
     3961 tcgcgatcgc caccgcttcg ccaccgctcg atccgccggg cgtcagatcg tgattcaacg
     4021 gatgattggt gcgtccaaac acgggattgt cagccgtgta gtccatcgcc atctcggcag
     4081 cgttactttt cccgaggacg atcgcgccgg cggctttaag tcgcgccacc gccggagcat
     4141 cagtttgtgg aacataatcc atgcgcatct tcgagccgct cgtcgtgcgc aagcccgcag
     4201 tttcgatcgt gtctttgatc gtcaccggca caccttgcaa cacaccattg cctgctcccc
     4261 gcatcaccgc gatttccgcc tcgcgcgcac gctctatcgc gtcaggcgcg agtgtcacga
     4321 tcgcattcag ctcgggattc aactctgcaa tccgctcgag atgcgcctcg acaacctcaa
     4381 ccggcgacac ctctcgcttc ctgatcatcg cggcgatggt ccctaacgtc tctttaatca
     4441 aatccgcgtt cttcatccgt ctaatccgtg ttttgcattt cctgctcttc ccaatataat
     4501 tcaatttgct ttattcagac ccaaacaacc tgccccatcg tgctctcggc aaaggagtga
     4561 attcttggct attcgtttga gcaggacctt cgtcttgcgc aagctccacc agctttccgg
     4621 tatcgttcca ctcggacttt tccttttaga acacttttac accaattcaa aggcgttgac
     4681 gggtgcgaac gacttcaaca agtccgtggt cgatctacag tcgatcccat acatcctgtt
     4741 cgtcgagatt ggcggtatct tcattccgct gatttatcac gccgtgtacg gactggtaat
     4801 cacaatggag gcgcggccca acaacctcgc gtacccttat tcgcgcaact ggttttacct
     4861 gatccagcga atcactgggg tcatcctctt cttttttatc acttttcacg tgctcaattt
     4921 ccgtttcggg atgatccccg gactgaatac agaatcagta gcgcatcatg ccgatcgggc
     4981 gtttgaaatc gtcgcgcgtg agtttcgcat gattccgatc tttctcgttt acatgatcgg
     5041 tatcaccgcg accgtctggc atcttgccaa cggcatctgg ttgtttctcg tcgattgggg
     5101 catcacgatc ggtgaacgcg cgcagcgttt gaccggatac gcgtgcatcg cgtttggcgt
     5161 ggtgctgctg ctggtcggca tcaacgccgc agtggcattc atccgtcccg gcggactgtt
     5221 aggaggaatt atttattaat ggccagtaac ggattgaaaa tcgcgattgt cggaggtgga
     5281 ctggcgggcc tcgcggccgc catgaaaatc gcggaggccg gccatgctgt cgatctgttt
     5341 tccgtcgtgc ccgtgaagcg ctcgcattcg gtgtgcgcgc agggcggaat caacggcgcc
     5401 gtcaacacca aaggtgaagg cgattcgacg tgggaacatt tcgacgacac tgtttacggc
     5461 ggcgactttc ttacaaacca gccacctgcg aaagcgatgt gcgagatggc gcccgcgatc
     5521 atctacctgt tcgatcgcat gggtgttcca ttctctcgta ctcctgaagg actactcgac
     5581 tttcgccgtt tcggcggtac aaaacatcac cgcactgcat tcgccggagc ttcaaccggt
     5641 cagcaactgc tctatgcact cgacgaacag gttcgtcgtt acgaagtaaa cggcaaagtc
     5701 agaaagtttg agcactggga gatgatgtcg ctcgtgctcg acgaacatca ggtgtgtcgc
     5761 ggattgatcg cgatggactt gcagacgctc gagttgaagg cgtttccggc tgacgcagtc
     5821 atcatggcga cgggtggtcc aggtctcatc ttcggcaagt cgacgaattc gatggtgtgt
     5881 accggcagcg ctgtctcggc gtgttaccaa cagggcgcga gatatgcgaa cggcgagttt
     5941 atccaggtcc atcccacttc gattccgggc gaagacaagt tgcgcttgat gtctgagtcg
     6001 gcgcgcggtg aaggcgggcg cgtttgggta cctcgcaaag aaggcgacgt tcgtcctcct
     6061 tcttcgattc c
//
LOCUS       DASSFQ010000061         9865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_921976, whole genome shotgun sequence.
ACCESSION   DASSFQ010000061 DASSFQ010000000
VERSION     DASSFQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921976"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..636
                     /locus_tag="VFH96_01840"
     CDS             79..636
                     /locus_tag="VFH96_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6972733.1"
                     /translation="MNFEEAVMPHLDAAYNLARWLTRNEADAQDMVQEAYLRALRFFG
                     GFHGTDARAWLLTIVRNTCYTWLKRSRSPELSGDLDEVMLTKESDEPDPEVSHFLKVQ
                     SELISEAIEALPIEFREVVILRELEELSYKEIASITGIPIGTVMSRLSRARKRLFVCL
                     RGVAPKQPAIKCRIKLVQAGNNRVA"
     gene            697..1161
                     /locus_tag="VFH96_01845"
     CDS             697..1161
                     /locus_tag="VFH96_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972734.1"
                     /translation="MKNVVVKMGLLAVMIVIAASVSANAQSLNYRLTANIPFDFSVSG
                     KKLPAGQYLINRAQVSDGDMVVQIRSTDGHSNVTRLTIPVSAHRPMKNGTLVFNRYGD
                     EYFLSEIWPAGGETGRVLHKSRAERELARKAQDSGVAALNAPNVQTVTIQVN"
     gene            1349..1705
                     /locus_tag="VFH96_01850"
     CDS             1349..1705
                     /locus_tag="VFH96_01850"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6972735.1"
                     /translation="MILAFTNSALAQTSRGYFVGNVLEQNGAVVKITNSSTRVTHVAT
                     STDDGSFRFDAIDPGTYRIEISQPGFKTITRDGLIISAAQTTTANFLLEAGTQTDRKR
                     KSWTYRAVSAPVRFLF"
     gene            complement(1697..3163)
                     /gene="guaB"
                     /locus_tag="VFH96_01855"
     CDS             complement(1697..3163)
                     /gene="guaB"
                     /locus_tag="VFH96_01855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET6972736.1"
                     /translation="MITNDIFEGLTFDDVLLIPDRSDVLPSQTDTATQFTRNIRLQIP
                     LCSAAMDTVTEAALAIALAQQGGIGVIHKNMSIERQAEEVDKVKRSESGMIVDPVTIR
                     PDRPVREALQVMERFHISGVPVVDEDGHLVGIITNRDLRFETRFDIPVSEVMTPQPLV
                     TVPVGTTLDQAKTVLQKHRIEKLLVVDEDKHIKGLITVKDIQKAIKYPTAAKDNLGRL
                     RVAAAIGATGDYRERADELVRTRVDCLVIDTAHGHSSRVIEAVKEIKKLHPETDLIAG
                     NVGTTAGAQELIDSGVDAIKVGIGPGSICTTRVVTGAGVPQITAISSCVKAARGQNVP
                     VISDGGVKFSGDVAKAIAAGADVVMIGSLFAGTEEAPGEVILFQGRSFKTYRGMGSLG
                     AMREGSRDRYAQDQIETDSKLVPEGIEGRVPYKGTLAEMVTQLVGGLRSGMGYTGCRT
                     IPEFQERTRFLRITSAGLKESHVHDVIITKEAPNYRLE"
     gene            complement(3160..3714)
                     /locus_tag="VFH96_01860"
     CDS             complement(3160..3714)
                     /locus_tag="VFH96_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6972737.1"
                     /translation="MAASIGEQLRLAREERGIGLREICEQTRISVHYLEAIEANDYKR
                     LPGGVFNRSFIKAYAKCVGYDEREAIEGYTRYLREHGESSDDINTTPLHSKVYTDTPA
                     TRSPVLTVILAILILAILTAGALAALHWFQRRASVTPARDVVIASLRISAPSALQFEL
                     NAEIRREPLRALNQSESPMYNDAT"
     gene            3968..4234
                     /locus_tag="VFH96_01865"
                     /pseudo
     CDS             3968..4234
                     /locus_tag="VFH96_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            4431..6176
                     /locus_tag="VFH96_01870"
     CDS             4431..6176
                     /locus_tag="VFH96_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6972738.1"
                     /translation="MSVDKDLVSVQQARDLVEAAHRAQAQVAEFDQAKIDRICEAMAR
                     AALSEAARLGAMAVEETGFGVADDKREKNRFAAEDVWNYFRGLRTKGIVSETKDVVEI
                     ASPRGVVAGIIPSTNPTSTAIFKILISIKSRNTVVLSPHPSAAKCINETARVMRDAGV
                     KEGLPADAIGCMTSATIEGTETLMKHKQTAVILATGGIGLVRAAYSSGKPAFGVGPGN
                     VPVCVERSADVPKAVQDILTGKCFDNGTICASEQAVICDAPVEQAVREQFKLQGAHFL
                     SAAEADQLGKVVATQQRSLNPAIVGKSVEVIAKMAGLSVPPGTRCLLADVAGVGRDHP
                     LSMEKLSPILAFYVVDGLQACAKRAQEVLAYGGMGHTAGIHTRSREAAVKFGAEMPAS
                     RITVNTPTTHGAIGFSTALPPSMTLGCGSWGGNVTSDNVSPLHLMDIKRVAFETRPVK
                     SARPAVSTQPVAASTKEPVVAGGKIDRQESAAIVDRFLAGKRIEPTASPLPPPVIESE
                     AGSMYTAPAPPPAPREVKPTPPSNNGAKAVEFVSEDDVRRAIQKGEKIYITGKTIITP
                     AARDMGEPAEVFAKV"
     gene            6180..6485
                     /locus_tag="VFH96_01875"
     CDS             6180..6485
                     /locus_tag="VFH96_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET6972739.1"
                     /translation="MFLAKVVGTVWSTKKTPDLEGVRFLIVHPYDLDKEPTRNIVVVA
                     DRLGAGAGEMVMCAFGKAARTAIGNQDMSIEAAVVGIVDRVDINESLSESSIPSNGQ"
     gene            6475..6921
                     /locus_tag="VFH96_01880"
     CDS             6475..6921
                     /locus_tag="VFH96_01880"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET6972740.1"
                     /translation="MANEELIYRITRAVYDRLGSAVDDTTVEQLVTDIYKQVETPGRS
                     VANGAAQLDSTEGSSARMVISVFGLDHPGIVAGVSQILAAASCSIVDINQTVVQGKFA
                     MVIIADTTRARESTAELKDRLRSEGEKLGVRIYAQREDLFNAMHRI"
     gene            6993..7328
                     /locus_tag="VFH96_01885"
     CDS             6993..7328
                     /locus_tag="VFH96_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6972741.1"
                     /translation="MSEPEIARRCVSCGVSVRQHAMFCPQCGKAIPEAAVTTATTPAD
                     TIIDHAPQPPPPARHQDGVKGRVEKLRHVSSIVIDQAAYDPSLRFLLVAAAMFLLFLV
                     LLIMSKVIG"
     gene            7329..8690
                     /locus_tag="VFH96_01890"
     CDS             7329..8690
                     /locus_tag="VFH96_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET6972742.1"
                     /translation="MEYTQAEILQTVAMTEMEHLDVRTVTLGLSLLDCKSDSMAGVAQ
                     KANEKIRAVAAQLVPVVNEIGEQFGIRVANKRISVTPVSLVTEGLRGDLMQVARAIDD
                     AAGAVGVDFLGGYSALVQKGTTPSEIRFLDSIPEALATTVRLCSSVNAATTRAGINMD
                     VVAQIGRIIKEAAERTRDRDGLACAKFVCFANAVEDNPFMAGAFHGVSEAEAVINVGV
                     SGPGVVHAAMRQADRNLPLQDLAEQIKKLSFKLARAGELVGREAARRLGIPFGIIDLS
                     LAPTPVPGDSVANIIEAMGFERAGTHGTTAALALLTDAVKKGGAMASGSVGGMSGAFI
                     PVSEDAGMIEAAQVGALSLAKLEAWTSVCSVGIDMVAVPGSTSAETIAAIIADELAIG
                     VMNNKTTAVRIIPVPGRDVGEMVEFGGLLGSAPIMPVNAFSSAAFIRRGGRIPAPIHS
                     LRN"
     gene            8829..9155
                     /locus_tag="VFH96_01895"
     CDS             8829..9155
                     /locus_tag="VFH96_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HET6972743.1"
                     /translation="MPEALGMVECRGLVAMIEAADAMVKAANVRLVGWEKIDAGLVTA
                     IVRGDVAAVRAAVDAGAAAGKRVGEVVATHVIPQPHSDVDLGLPVLHTGETTRKKISG
                     SVRAKD"
     gene            9224..9697
                     /locus_tag="VFH96_01900"
     CDS             9224..9697
                     /locus_tag="VFH96_01900"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET6972744.1"
                     /translation="MPPKRNKISGFLEKFSHQATKATGTSVAFVTAVLVIVVWGITGP
                     LFHYSDTWQLVINTGTTIVTFLMVFLIQRAQNKDALAIHLKLNEIVAAMEGASNRLID
                     VEDLSEPEIDALRNYYKRLIELARKDDQLTQTHSIEEAASRHGFKYKKKAADKPK"
BASE COUNT         2130 a         2620 c         2950 g         2165 t
ORIGIN      
        1 acctgggacg catccgcgct gctttgcagg agagaacgct ggcgaaattg accgcgtgat
       61 tttttcacgg tggaaaccat gaacttcgaa gaagcagtta tgccgcatct tgacgccgcc
      121 tacaacctgg cgcggtggtt aacgcgtaac gaagccgacg cgcaggacat ggtccaggag
      181 gcttatctgc gcgcgctccg gttctttggt ggtttccatg ggactgacgc gcgcgcatgg
      241 ttgttaacga tcgtgcgaaa cacctgctac acgtggttga agcgtagtcg gtcgccggag
      301 ctgtcaggcg acctcgatga agtgatgttg acgaaagagt ctgacgagcc tgatccggaa
      361 gtttcgcatt tcttgaaagt tcaatccgaa ttgatcagcg aagctatcga ggcactgccg
      421 atcgaatttc gtgaagtcgt gatcctgcgt gagctcgaag agctttcgta caaagagatc
      481 gcctcgatca cgggaattcc gatcggcacg gtcatgtcgc ggctctcacg tgcgcgtaag
      541 cggctatttg tttgcctgcg tggcgtggca ccgaagcaac ccgctatcaa gtgccggatc
      601 aagttggtcc aggcggggaa taaccgagtc gcctgatcgg taattagatc agaagcgatt
      661 caaagacttg attgagagtt aaagaggaga gttgttatga agaacgtagt tgtaaagatg
      721 ggtttgttgg cagtgatgat cgtcatcgcg gcgagcgtgt cagcaaatgc gcagtcactt
      781 aattaccggt taaccgcaaa cattccgttc gatttttcag tgtccggcaa gaaactcccg
      841 gcgggtcagt atttgatcaa ccgtgcgcaa gtgagcgacg gagatatggt ggttcaaatc
      901 aggagtacag atgggcactc gaacgtaact cgtctgacga tcccagtctc cgcacacaga
      961 ccaatgaaga acggcacgct ggtgtttaac cgttatggag atgagtactt cctttcggaa
     1021 atctggcctg cgggtggtga aacgggacgg gtattgcaca agtcacgtgc ggaacgcgag
     1081 ctcgcgcgca aagcacagga cagtggagtc gcggcactta acgcgccgaa tgtgcagaca
     1141 gtgaccattc aggtgaatta accggccgta aagtttcgag tgactctctg aagtgcgccg
     1201 gcctaccgct gcgggggcgc atagcgagcg aggcggtcgc acttcagaag tcctcgaatc
     1261 aatccgcttt tatccgcgtg aatctgtggc taagaaaaag taaggagtgt tggttgtgag
     1321 gagatttgga tatacagcaa tcgttgtgat gattctggct tttaccaaca gcgctttggc
     1381 ccagaccagc cgaggatatt tcgtcggcaa cgttctcgag caaaatggcg cggtcgtaaa
     1441 gatcacaaac tcgagcaccc gcgtcacgca cgtagccaca tcaactgacg acggcagctt
     1501 tcgtttcgac gccatcgatc ccggcactta caggatcgag atcagtcaac ccggtttcaa
     1561 aaccatcaca cgcgatggcc tgatcatctc cgccgcccag accacaaccg ctaacttcct
     1621 gctcgaagcc ggcacgcaga cggatcggaa acgtaaatcc tggacctatc gagccgtttc
     1681 agctccggta cgtttcttat tctagtctgt aattgggcgc ctctttggtg atgatcacat
     1741 cgtgtacgtg agattctttc aaccctgctg acgtgatgcg gaggaagcgc gtccgctcct
     1801 ggaattccgg gatcgtccgg cagcccgtgt agcccattcc cgatcgcagt ccaccaacga
     1861 gctgcgtcac catttccgcc agcgtgcctt tgtacggcac gcgtccttca attccttccg
     1921 gaacgagttt cgagtcagtc tcgatctggt cctgcgcgta acgatcgcgc gagccttcgc
     1981 gcatcgcgcc gagcgagccc atgccacggt acgtcttgaa cgagcgtccc tggaatagaa
     2041 tcacttcgcc gggcgcttct tctgttccgg caaacaacga tccgatcatc acgacgtctg
     2101 cgcccgctgc gatcgccttc gccacgtcgc cggaaaactt cacgccacca tcggagatta
     2161 caggaacgtt ctgtccacgc gccgctttca cacaactcga gatcgcagtg atctgcggta
     2221 cacccgcgcc agttacaact cgcgtcgtac agatcgatcc cggaccaatt ccgactttga
     2281 tcgcatcgac tcccgagtcg atcaactcct gcgcgccggc agtcgtgcca acatttcccg
     2341 cgattaagtc cgtttccgga tgcagcttct tgatctcttt aaccgcctcg atgacgcgcg
     2401 atgaatggcc gtgtgcagtg tcgatcacga gacaatcgac gcgtgtcctc acgagctcat
     2461 ccgcgcgctc gcgataatcg ccggtggcgc cgatcgcggc cgcgacgcgc aaacgtccga
     2521 gattatcttt cgccgccgtt ggatacttga tcgccttctg aatgtctttg acggtgatga
     2581 gtccttttat atgtttatct tcatcgacga cgagtagctt ctcgatgcga tgtttttgca
     2641 aaacagtctt ggcctgatcg agcgtcgtgc ccacgggaac agtgacgagc ggctgcggcg
     2701 tcatcacttc ggagacggga atgtcgaacc gcgtttcgaa tcgcagatcg cgattagtga
     2761 tgatgccgac gagatgacca tcttcatcga ccaccggcac gccgctgata tgaaaacgct
     2821 ccattacctg cagcgcttca cggaccgggc gatccggtct aatcgtcacg ggatcgacga
     2881 tcattcccga ctcggatcgt tttactttat cgacctcttc ggcttgtcgc tcgatactca
     2941 tgtttttatg aatgacaccg atgccgcctt gctgcgcgag tgcgattgcg agcgctgctt
     3001 cagtcaccgt atccatcgct gccgaacaga gtggaatctg tagccgaatg ttgcgcgtga
     3061 attgagtggc ggtgtcggtt tgggagggta gaacgtcgga acgatctggt attaaaagta
     3121 cgtcgtcgaa ggttaagcct tcaaaaatat cattggtaat catgtggcgt cattatacat
     3181 aggtgactcc gattggttga gcgctctcag cggctctctg cgtatctcag cgttaagttc
     3241 gaattgcaac gcagagggcg cagagatacg cagagatgca atcaccacat ctcttgcagg
     3301 cgtcaccgag gcgcggcgtt ggaaccagtg cagggcggcg agcgccccgg cggttaagat
     3361 tgccagaatt aaaatcgcca ggatcacggt cagcacaggt gagcgtgtgg ccggtgtgtc
     3421 tgtgtagact ttcgaatgca gcggcgtggt gttgatgtcg tcactcgact cgccgtgctc
     3481 gcgcaaataa cgcgtgtaac cttcgatggc ttcgcgctcg tcgtagccca cacacttcgc
     3541 gtaggccttt ataaaactgc gattgaaaac tccgccgggc agacgcttgt agtcgttggc
     3601 ttcaatggcc tcgagataat ggaccgagat ccgcgtttgc tcgcagattt cgcgcagacc
     3661 aatgcctcgc tcctcgcgcg cgagtcgcaa ttgttcgcct atactcgctg ccatctccac
     3721 tcgctcgatt cctggtgtgt gtggtcgggg attgttaacc caggtctatc agcgcgccgc
     3781 ttgtatgaat gcgtacaact cccacttttt ctgcactata gcttgcgctg caaaacgttg
     3841 tcaaactttt gtatctcaaa agatatgcgt ctcttgcttc ttgccgcccg cggtggtacg
     3901 ttgacgccac agtgacccga tcaagtcgct tttaatatct acaatagata aaagtgagga
     3961 caaagagatg cccctggaag cactcggaat ggttgagacc aaaggattcg tgggtgcagt
     4021 tgaggccgcc gatgccatgg tcaaggcagc gaatgtacaa ctgctcggga aggagtacat
     4081 cggcgccggc tacgtaacga tctttgtgcg cggcgacgtt ggtgcggtca aagcggcaac
     4141 tgacgcaggc gcggcggcgg cccggcgcgt tggtgagctg atcagcgtac acgtaatccc
     4201 gaggccacac gcggaagtcg agcgcgtgct gccggtcaac ggtcgcactg gcctgagtcc
     4261 cgacgaacag gcgctgcaag gtggtggcgc acgcggccag ctgggccagg gagatcaggg
     4321 acgatcgctc ggcgcgggaa cgcgtgacgc gaacaaagag cgagcaagat agttcatcca
     4381 cagattacgc tgattacaca gaaacctgtg gataagatgt ggagtcagtc atgtccgtcg
     4441 ataaggattt agtttcagtg caacaggcgc gcgacctcgt cgaggcagcg caccgggcgc
     4501 aggcgcaagt tgcggagttt gatcaggcca agatcgatcg catctgtgag gcgatggcgc
     4561 gggcggcgct gagtgaagcg gcacggctcg gcgcgatggc cgtcgaggaa actggatttg
     4621 gcgttgccga tgacaagcgg gagaagaacc gcttcgcggc ggaagatgtt tggaactact
     4681 ttcgcggact acgtacgaag ggcatcgttt ccgagacgaa agacgttgta gagatcgcca
     4741 gcccgcgagg tgtcgtggcc ggcattattc cttccactaa tccaacctcg actgcaattt
     4801 tcaagatcct gatctcgatc aagtcacgca ataccgtcgt gctgtcgccg catccgtcag
     4861 ccgcgaagtg catcaacgaa acagctcgag tcatgcgcga cgcgggggtg aaggaaggtc
     4921 tgcccgccga cgcgattggc tgcatgacat ccgcgacgat cgaaggcact gagacgttga
     4981 tgaagcacaa gcagacggcc gtgatactag cgaccggcgg catcggactg gtgcgcgcgg
     5041 cgtattcgtc aggtaaaccg gcgtttggcg ttggaccagg caacgtgccg gtgtgtgtcg
     5101 agcgcagtgc cgacgtgccg aaggccgtgc aggatattct gactggcaag tgttttgaca
     5161 atggaacaat ctgcgcttcc gagcaggcgg tgatttgcga tgcgcccgtc gagcaggctg
     5221 tgcgcgaaca gttcaagctt cagggcgcac attttctttc cgccgccgaa gcggatcagc
     5281 tcgggaaggt ggttgcgact cagcagcgtt cgctgaatcc ggcgatcgtc ggcaagtcag
     5341 ttgaagtgat cgcgaagatg gcggggctca gtgttccgcc aggcacgcgc tgtttgctcg
     5401 cggacgttgc cggtgtcggc cgcgatcatc cgctctcgat ggagaagctg tcgccgatcc
     5461 tcgcgtttta tgttgttgat ggtttgcagg cgtgcgcgaa gcgtgcgcag gaagtgctgg
     5521 cgtatggcgg catgggccac actgcgggaa ttcacacgcg ctcgcgtgaa gcggcggtga
     5581 agtttggcgc cgagatgccg gcgtcgcgta taactgtgaa cacgccgacg acgcatggcg
     5641 cgatcggttt ttcaacggca ttgccgccgt cgatgacgtt gggttgcgga tcgtggggcg
     5701 ggaatgtgac gtcagataac gtgtcgccgc tgcatttgat ggatatcaag cgtgttgctt
     5761 ttgagacgag gccagtgaag agcgcgcgtc cggcagtttc gacgcagccg gttgccgctt
     5821 caaccaaaga accggtagtt gcgggcggta agatcgatcg acaagagagt gctgcgatcg
     5881 tagatcgttt cctggccggc aagcgcattg agcccacggc ttcaccattg ccaccgccgg
     5941 taatcgagag tgaagcggga agtatgtaca ccgcgcccgc gccgccacca gcgccacgtg
     6001 aggtcaaacc gactcctcca tcaaacaatg gagcgaaggc agttgagttt gtgagtgaag
     6061 acgacgtgcg ccgcgctatt cagaaaggcg agaagatcta cattaccggg aaaacgatca
     6121 tcacgcctgc cgcccgcgat atgggcgaac cggcagaagt ctttgccaaa gtgtaattaa
     6181 tgtttctagc aaaagttgta ggcacagtct ggtcgacaaa gaagactccc gatctcgagg
     6241 gcgtcagatt tctcattgtt catccttacg atctcgacaa ggagccgacg cggaatatcg
     6301 tcgtggtcgc cgatcgactc ggcgcgggcg ccggtgagat ggtgatgtgc gccttcggca
     6361 aggccgcgcg aacggcgatc ggtaaccagg atatgtcgat cgaggccgcg gtcgtcggca
     6421 tcgtcgatcg tgtcgacatc aacgaatccc tatccgaatc atccatccct tccaatggcc
     6481 aatgaagagc tgatttaccg aataacccgg gcggtttacg atcgcctcgg cagtgcggtt
     6541 gacgatacga ctgtcgagca gctagtcacc gatatctaca agcaggtcga gacgccgggc
     6601 cgcagtgttg ctaacggtgc ggcgcaattg gattcgacgg aaggttcgtc ggcgcgcatg
     6661 gtcatttcgg ttttcgggtt ggaccatccg ggaatcgtcg cgggcgtgtc gcagatactg
     6721 gcggctgcga gttgcagtat cgtcgatatc aatcagactg tcgtgcaggg aaaattcgcg
     6781 atggtgatca ttgctgacac gacgcgcgcg cgtgaaagca ctgcggagtt gaaagatcga
     6841 ttgcggagtg aaggggagaa actcggggtg cggatttacg cgcaacgcga ggatcttttc
     6901 aacgccatgc accggattta gacaggatgt caggatttgc aggattcatt ttgtaaatca
     6961 tgaaatcttt gtaaatcctg tccaaaggtt ttatgtcaga acccgaaata gctcgtcgtt
     7021 gtgtatcgtg tggcgtgtca gtgcgccagc acgcgatgtt ctgcccgcag tgtgggaagg
     7081 cgattccaga ggcggcagta acaacagcga ccactcctgc tgacacaatc atcgatcacg
     7141 ctccgcaacc accacctccg gcgcgacatc aagacggtgt caaaggccgg gtggagaagt
     7201 tgcgtcacgt ttcgagtatc gtgatcgatc aggcagcgta cgatccgagt ttgcgtttcc
     7261 tgctcgtggc tgcggcgatg tttctgctgt tcctcgtgct cctgatcatg agcaaagtca
     7321 tcggatagat ggaatacact caagccgaga tcctgcaaac agtcgcaatg accgagatgg
     7381 agcatctcga cgtgcgcacc gtcacgctcg gtctgagttt gctcgactgc aagagcgatt
     7441 cgatggcggg tgttgcgcaa aaagcaaatg agaagatccg cgcggttgcc gcccagctcg
     7501 tgccggtagt taacgagata ggcgaacagt ttggaatcag agtcgccaac aaacgcatct
     7561 ccgtcacgcc tgtgtcgctc gtcactgaag gactgcgagg cgatctcatg caggtcgcac
     7621 gcgcgatcga tgatgccgca ggcgcagtcg gtgttgattt cctcggtggc tactccgcac
     7681 tcgttcaaaa aggcaccacc ccttcagaga tccggtttct tgattcaatt ccggaagctc
     7741 tggccacgac ggtgcgtttg tgttcgtccg tgaacgccgc gacgactcgg gccggcatca
     7801 acatggacgt ggtggcccag atcggcagga tcatcaagga agccgccgag cggacgcgcg
     7861 atcgagacgg actcgcgtgc gcgaagtttg tctgcttcgc gaatgcggtc gaagacaatc
     7921 cattcatggc cggcgctttt catggcgtga gtgaagctga agccgtgatc aatgtcggtg
     7981 tgtcgggtcc gggcgtggtc catgccgcga tgcggcaggc ggatagaaat cttccgttac
     8041 aggatctcgc cgagcagatc aagaaactgt cgttcaagct ggcgcgtgcg ggcgagctcg
     8101 tcggacgtga agcggcgcgt cgactcggaa ttccgttcgg catcatcgat ctttctttag
     8161 caccgacacc tgttcccgga gattcagttg ccaacatcat cgaggcgatg ggcttcgaac
     8221 gcgcaggcac gcacggcacg actgcggcgc tcgcgttgtt gactgatgca gtgaagaaag
     8281 gcggcgcgat ggccagcggc tctgtcggcg gcatgagcgg cgcgtttatt ccggtgtcgg
     8341 aagatgcggg catgattgag gccgcgcagg tcggcgcctt atcgctcgcg aaactcgaag
     8401 catggaccag cgtctgctcc gtcggcatcg acatggttgc cgtgcccggt tcgacttcgg
     8461 ctgagactat cgcggcgatc atcgctgacg agctggcaat cggtgtcatg aacaacaaga
     8521 cgactgcggt gcggatcatt ccggtgccgg gtcgtgatgt cggcgagatg gttgagtttg
     8581 gcggtttgtt gggcagcgcg ccgatcatgc cggtcaacgc tttttcatcc gccgcattta
     8641 ttcgacgagg tggccgaatt cctgcaccaa tccactctct gcgaaactag accacaaaag
     8701 gcacaaagta gacacagaag gcacaaaggc agaaaccctt tttcagaaaa aacttttgtg
     8761 ctttttgtgg gcaaagcttt gtgccttttg tggtccggtt tgttactatt gcggcacctg
     8821 gagggtttat gcctgaagca ctcggaatgg tcgaatgccg tggcctggtg gcgatgatcg
     8881 aagctgccga tgcgatggtg aaagctgcga atgtgcgcct cgttggttgg gaaaagatcg
     8941 acgcgggcct ggttacagcc atcgtgcgcg gtgatgtggc ggctgttcgc gctgccgtcg
     9001 atgccggcgc tgctgcagga aagcgagtcg gcgaagttgt ggcgacgcac gtgatccctc
     9061 aaccccactc ggatgtggat cttggtttac cagtcctgca cactggcgaa accacgcgca
     9121 agaagatcag tggttctgtc agagccaaag attgatcccc tgaagtaatt ccccaaatct
     9181 ttccctcaat ctgttccgcc cggaaatttg tggagaatta tcgatgccgc caaaaagaaa
     9241 taaaataagt ggattcctcg aaaagttttc tcaccaggcc acgaaagcta caggaacatc
     9301 ggtcgctttt gtgactgcgg tattagtgat cgttgtctgg ggcatcactg gaccactctt
     9361 tcactattcc gacacctggc agctcgtgat caataccggc acgacgatcg tcactttcct
     9421 gatggtcttt ttgattcagc gcgcgcagaa caaagacgcg cttgccatac atctgaagtt
     9481 aaacgagatc gttgcggcga tggagggcgc gagcaaccgc ctgatcgatg tcgaggattt
     9541 gagtgagccg gagatcgatg cgttgcggaa ttactacaag aggttgattg aactcgcgcg
     9601 caaggatgat caactcacgc agacgcactc gatcgaggaa gcagcctcga gacacggctt
     9661 caagtataaa aagaaagccg cagataaacc caaatgaacg cggatctgcg attagggctt
     9721 gcttggagtg gtcggcatgc gcactgccgg tggacgcacg ttgccttgat tctctttgcg
     9781 tgcgagacgg ccggtgcgcg ttaaggcaat gcgcttgtct gaattggccg gctggtccac
     9841 cgtcactttg atcgcgcgat agctg
//
LOCUS       DASSFQ010000062         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1515643, whole genome shotgun sequence.
ACCESSION   DASSFQ010000062 DASSFQ010000000
VERSION     DASSFQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515643"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..863)
                     /locus_tag="VFH96_01905"
     CDS             complement(87..863)
                     /locus_tag="VFH96_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6972745.1"
                     /translation="MASTFQNIHFRIDDRVARITFARPPLNVFNIEMMREIASALSDC
                     NQREVVAIVFDADKNCRAFSAGVAVEDHVQDTIFQMLDEFHSIFRLLEQIAKPSIALI
                     DGAALGGGCELVAACDIVLASDRSRFGQPEIKLGVFPPVASVLLPLVIGEKRARELIL
                     TGEIIDAVEAARLGLCNHVVPGAHLEAKLNEVLAKLRELSGTSLSYARQSLDLGRGRS
                     IEDALNEQENIYLHELMKTQDANEGVKAFMEKRKAIWRHR"
     gene            complement(950..2800)
                     /locus_tag="VFH96_01910"
     CDS             complement(950..2800)
                     /locus_tag="VFH96_01910"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6972746.1"
                     /translation="MLRRRVVCAVAFLFVLPFLAAAQTAPVDISFTVAMSRPHTHLFD
                     VDVAIKRTANGPQEESLVMPVWTPGSYMVREFARNVQDFAANAAGQPLKWEKTNKNTW
                     RVVTNGATEWHATYRVYANELSVRTSELNSGHAFWNNANLLMYLDGFLKSPSTVRVLA
                     PEVWKVATGLPVVLGQKNTFRAENFDILYDSPFEASNFKTIVFNVKGVPHRIVIDGEG
                     NYDPERMRVDVQKIVETQVALMGEIPYKDYTFILHLRSNAGGGLEHLNSTALGYPRFG
                     FRIRTGERTTSAAPNAADNFERDYRGFLALVSHEFFHLWNVKRIRPDALGPFDYTQEN
                     YTKMLWVAEGITDYYADIALRRAGLVSEGDFLSATARSMWSLQNTPGRLEQTVEESSF
                     DAWIKYYRQDENSNNSQISYYDKGALLGLLLDLEIRKRTSGAKSLDDVMRYLYAEYFK
                     KNRNYGPSDFQKACELMAGASLDDFFNKYVRGIAELDYNAALDAAGLRLDTSAGAGSV
                     YFGADVAQDDDRLIVRRVYAGSPAYEQGLNAGDQIVAMDNMRVTRDFFNARMAEKKPG
                     DLINLTIFRFDDLSTLLIKLGARSEGAYRIVPLPNATDVQKRIYRGWLGV"
     gene            2929..3366
                     /locus_tag="VFH96_01915"
     CDS             2929..3366
                     /locus_tag="VFH96_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6972747.1"
                     /translation="MFRRLIATSPAWFTIPIRLGLAAVMIAHGAQKVLGSFNGPGFKT
                     FISGTTPYGFMRPAWLWLAAAALSELIGGVLVGLGFLTRLGAFFIACVMLTAVVGVHL
                     PGGFFAANRGYEYPLVLLAMSLALLISGGGQASVDRALSGGRR"
BASE COUNT          741 a          962 c          901 g          808 t
ORIGIN      
        1 cgcaaaggac cagaataaga cgccaagaag aagctttgcg cctttttctg gtcctttgcg
       61 cctttgcgtg aaatattctt catagactac ctatgccgcc agattgcttt ccgcttctcc
      121 atgaacgcct tcacaccctc gttcgcgtct tgagtcttca tcaactcgtg cagatagatg
      181 ttttcctgtt cgttgagcgc gtcttcgatc gaccgtccgc gtccgagatc cagcgactgg
      241 cgtgcgtaac tgagcgacgt gcccgatagt tctctcaact ttgccagcac ttcgttcagc
      301 tttgcttcga gatgtgcgcc gggcaccacg tggttgcaca gccccagtct cgccgcctca
      361 accgcatcga ttatctcgcc agtcaggatc aattcgcgag cgcgtttttc gccaatcacg
      421 agcggcagca acaccgacgc taccggcggg aatacgccca gctttatctc gggctgtccg
      481 aaacgcgatc gatcggatgc caggacgata tcgcatgcag ccaccagttc acagccacca
      541 ccaagcgcag cgccgtcaat caacgcaatc gacggcttag cgatttgttc gagaagacga
      601 aagattgagt ggaactcgtc gagcatttga aagatcgtgt cctggacgtg atcttcaact
      661 gcaacgccgg cactgaatgc acgacagttc ttatccgcgt caaaaacaat cgccactact
      721 tcacgttgat tgcagtcgct gagcgcgctc gcgatctcgc gcatcatctc gatgttgaag
      781 acattcagcg gcggtcgcgc gaaagtgatt cgggcaactc gatcgtcaat tcggaaatgg
      841 atgttttgga atgtggatgc catgcgtttg cttcacagga tgaacaagat tttattcagg
      901 attaacttgt aaatcctgct caaatctttt tcatcctgtc caactgcgac taaacaccta
      961 accagcctcg ataaatgcgc ttctgtacgt ccgtcgcgtt cgggagtggg acgatgcgat
     1021 atgctccttc gctgcgcgcg ccgagtttga tcaataaggt gctcagatcg tcgaagcgaa
     1081 aaatcgtcag attaatcaga tcgccaggct tcttctccgc catccgcgcg ttgaagaagt
     1141 ctctcgtcac gcgcatgttg tccatcgcaa cgatctgatc gccggcgttc aatccctgtt
     1201 cgtacgcggg cgagccggcg taaacgcgtc gcacgatcaa tcgatcgtca tcctgcgcga
     1261 cgtcggcgcc aaaatacacg ctacccgcgc cagcgctcgt atcgagccgt agccctgctg
     1321 catcgagcgc ggcgttgtaa tcaagctctg ctatgccacg cacgtacttg ttgaagaagt
     1381 cgtcaagact cgcacctgcc atcagttcgc acgctttctg aaaatccgac ggcccgtaat
     1441 tgcgattctt cttgaaatat tccgcgtaca ggtaacgcat cacgtcgtcc agcgacttcg
     1501 ccccactggt tcgtttgcga atctcaagat cgagcagcaa tccgagcaac gcgcctttgt
     1561 cgtagtacga aatctgagag ttgttcgagt tttcatcctg tcggtaatac ttgatccacg
     1621 cgtcgaagct cgactcttca acagtctgct ccagccgtcc cggcgtgttc tgcaacgacc
     1681 acatcgatcg cgctgtcgca ctcagaaagt cgccctccga gaccaatcca gctcgacgca
     1741 gagcaatgtc ggcgtagtaa tcggtaatac cttccgccac ccaaagcatc ttcgtgtagt
     1801 tttcctgcgt gtaatcaaac ggtccgagcg cgtcgggacg aattcgcttc acgttccaga
     1861 gatgaaagaa ctcgtgcgac acgagcgcga gaaaaccacg atagtcgcgt tcgaaattgt
     1921 cggcagcatt gggcgcggcg gacgtggtac gctcgccggt gcgaatgcgg aaaccaaaac
     1981 gcggataacc gagcgcggtg gaattcaaat gttcgagtcc accgcccgca tttgatcgca
     2041 gatgcaggat gaaggtgtag tctttgtaag gaatctcgcc catcaacgct acttgcgttt
     2101 cgacgatctt ctgcacgtcg actcgcattc gctccggatc gtaattgcct tcgccatcga
     2161 tcacgatgcg atgcggaacg cccttgacgt tgaacacgat cgttttgaaa ttgctggctt
     2221 cgaaaggcga gtcgtaaaga atgtcgaaat tctcggcgcg gaaagtattt ttctgtccca
     2281 gcaccaccgg caagcctgtc gctactttcc aaacctcagg cgcgagcacg cgcacggttg
     2341 aaggactctt cagaaatccg tcgagataca tcaacaggtt cgcgttgttc cagaacgcat
     2401 gacctgagtt caactcactc gtgcgcactg acaattcgtt cgcataaaca cgataagtcg
     2461 catgccattc agtcgcgcca ttggtgacaa cgcgccacgt gttcttgttt gtcttttccc
     2521 atttcaacgg ctgaccggct gcgttggcgg cgaagtcctg cacgttgcgc gcgaattcac
     2581 gcaccatgta cgagccgggc gtccacacgg gcatcacgag cgactcctct tgcggaccgt
     2641 tcgcggttcg tttgatggcg acgtcgacat cgaacaagtg cgtgtgtggc cgcgacatgg
     2701 ccacggtgaa cgaaatatcg accggcgctg tctgtgcggc tgcaagaaac ggaagaacga
     2761 acaggaaggc gactgcgcac actactcggc gtctgagcat tcggtaatca tctttcgaaa
     2821 agttgataac aagtacgatt gaatgttact acgaagcggt gcgatccggg caaggttact
     2881 gttactattc ccccgctcta ctgaaacaac ttcgggaaca caacatcaat gtttagacga
     2941 ctgattgcga cctctccggc gtggtttact attccgattc gcctggggct ggcggcagtg
     3001 atgatcgcgc acggcgcgca aaaagtgttg ggcagcttca acgggccggg cttcaagaca
     3061 ttcatttcgg gaaccacgcc gtatggtttc atgcgaccgg catggctctg gctcgcagcc
     3121 gccgcgcttt ccgagctgat aggcggcgtg ctcgtgggac ttgggttttt gacgcgcttg
     3181 ggagcgttct tcattgcgtg cgtgatgcta acggcggtgg tgggagtgca cttgccgggc
     3241 ggcttctttg ctgcaaaccg tggttatgaa tatccattag tgctgcttgc aatgtcgctc
     3301 gcgttgctga tctcgggtgg cggacaagcg tcagtagata gagcactgag tggaggtcgt
     3361 cgttaaattg agcgccacct tggagtgcgc caacttgttg gcgctttggt at
//
LOCUS       DASSFQ010000063         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1515736, whole genome shotgun sequence.
ACCESSION   DASSFQ010000063 DASSFQ010000000
VERSION     DASSFQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515736"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFH96_01920"
     CDS             <1..260
                     /locus_tag="VFH96_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6972748.1"
                     /translation="ENAGLQMNDEIVSLAGKPVTRDTWRRTLARFKPGDSVPITVKRD
                     RRTIKANIVLGQPERFDYRIEEKADATAEQKQLRAAWLNGK"
     gene            complement(279..1172)
                     /locus_tag="VFH96_01925"
     CDS             complement(279..1172)
                     /locus_tag="VFH96_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6972749.1"
                     /translation="MSPRKATLISNPKTGRYVARRRPVEELAAQLESLGVKVDLRLTH
                     GPGDATEIASRAARNGSSEVIVAGGDGTINEAIQGLAGTKARLAIIPRGTGNVLAHEL
                     GLPLDDEQALQIAAQGKTRQIYLGLAIDETSNESRYFILMAGIGLDASVVRRVQPRLK
                     KRIGKGAFWVSGLSHLATWNPRPFILEINGHNYTSTFAAIGKAAKYGGDLAITPGARL
                     DQPEFEVCIIDTRSRLRYLHLLSQAMRNGMPRDTPEVQFVNAVTIKAHGDAHVQIDGE
                     LIGRLPMRFEIAPHSLEIIVP"
     gene            1389..1730
                     /locus_tag="VFH96_01930"
     CDS             1389..1730
                     /locus_tag="VFH96_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET6972750.1"
                     /translation="MLTKRHFWLVISAALLTLAFTYGRGTGAQPADANEIVIQNFAFE
                     PATLTVKVGTKVTWINRDDEPHTATATDKRFNSKTLENGDRFAMEFNQPGIYKYYCAL
                     HPKMTGQVIVK"
     gene            1742..2674
                     /locus_tag="VFH96_01935"
     CDS             1742..2674
                     /locus_tag="VFH96_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6972751.1"
                     /translation="MKQDQIERRGFLKCMAWAGTGLVWAMNGGVLSSELLAAPKRKAG
                     FSFVQISDSHIGFNRDPNHDVTKTFQEAVDKINALEERPAFVIHTGDITHLATAEQFD
                     TAAQIMKSAKADRVYYVPGEHDVFTDDGKLYFERFGGGPGWRSFDFNGVHFIGLINVL
                     NLKQGTGTGLGGSGLGQLGQEQLAWLQKDVKGLSASTPIVVFAHIPLWTIYPDWGWGT
                     DESGQALSYLKRFGSVTVLNGHIHQVLQKVEGNIAFHTARSTAYPQPAPGTAPSPGPI
                     KDVSAEKLRSTLGVTDVSYMAKVGPLAVVDEALG"
BASE COUNT          644 a          704 c          844 g          605 t
ORIGIN      
        1 ctgaaaatgc gggattgcag atgaacgatg agatcgtttc gctagccgga aaaccagtga
       61 cgagagacac gtggcgtcgg acgctcgcgc ggttcaagcc aggcgactca gtgccgatca
      121 cagtcaaacg cgatcgacga acgatcaagg cgaatatcgt gttgggccaa ccggaacggt
      181 ttgattaccg gatcgaggag aaggcggatg cgactgccga gcaaaagcaa ttacgtgctg
      241 cgtggttgaa tggaaaatga gccgcggatg aaacggattt aaggcacgat tatttccaac
      301 gagtgcggcg cgatctcgaa gcgcatgggc aggcgaccga ttaactcccc atcgatctgc
      361 acgtgtgcgt cgccgtgggc tttgatcgtt acggcgttta caaactgtac ctcgggagtg
      421 tcgcgaggca tgccgttgcg catcgcttgc gatagcaggt gcaggtaacg caggcggctg
      481 cgcgtgtcga tgatgcaaac ctcaaactcg ggttgatcga gtcgcgcgcc cggcgtgatc
      541 gcaaggtcgc cgccgtattt tgccgcttta ccaatcgctg caaacgtcga cgtgtaattg
      601 tggccgttga tttcgagtat gaatggccgc gggttccagg ttgcgagatg actcaatcct
      661 gaaacccaaa acgcgccttt gccgattcgc tttttcaacc tcggttgcac gcgcctcacc
      721 accgacgcgt cgagaccgat gccggccatc aaaataaaat agcgactctc gttgctggtc
      781 tcatcgatcg cgaggccgag atagatctgc cgcgtcttac cctgcgccgc gatctgcaac
      841 gcttgttcgt cgtcgagcgg cagaccgagc tcatgcgcga gaacgtttcc ggtgccgcgc
      901 gggatgatcg cgagtcgcgc cttcgtgcct gctaaaccct ggatcgcttc gttgatggtg
      961 ccatcaccgc cagcgacgat cacctctgag ctgccattac gcgcagcacg cgaggcgatc
     1021 tcagtcgcgt cgccggggcc gtgcgtcaaa cgcaagtcga ctttgacgcc gagcgattcg
     1081 agttgtgcgg cgagttcttc aaccggtcgc cggcgtgcaa cgtagcggcc ggtcttcgga
     1141 tttgaaatca gtgttgcctt acgaggagac atcgatcacc agtgagcaac ggcaatcgaa
     1201 gtgccgcgcg cgtttgccaa tcgccatgcg catgacgttc cagcgttact gccaaaagca
     1261 gacagtagtc cgataaatca gtcacagaga aacagtacag ccgataccgg ttaagtacaa
     1321 cttcgggaat aactttcgtt gctgctcagt atgcatcgtc gggaaaataa tatttggagg
     1381 ttttcgcaat gcttacgaag cgacatttct ggctcgtgat atccgcggct ctgctaacgc
     1441 tggcttttac ttatggtcga ggaacgggag cacagcccgc ggacgcgaat gagatcgtca
     1501 ttcaaaattt tgcatttgaa ccagccacac tgacggtcaa ggttggtaca aaagttacct
     1561 ggatcaatcg cgacgacgaa ccgcacaccg cgactgccac tgacaaacgt ttcaactcaa
     1621 agactctgga gaacggcgac cggttcgcaa tggagttcaa tcagccgggc atttacaagt
     1681 actactgcgc gctacatccg aagatgacgg gtcaggtcat cgtcaaatag gagaaacgaa
     1741 agtgaaacaa gatcaaattg agcgacgcgg ttttctcaaa tgcatggcgt gggcaggtac
     1801 cgggctcgtt tgggcgatga atggcggcgt actgagctcg gagctgctcg cggccccgaa
     1861 gcggaaagcc ggcttcagct ttgtccagat cagcgatagt catatcggat tcaatcgaga
     1921 tccgaatcac gatgttacaa agacttttca agaggcggtc gataagatca acgcgctcga
     1981 ggagcggccc gcgtttgtga tccacacagg cgacatcacc catcttgcta cggccgaaca
     2041 gttcgatacg gccgcacaga tcatgaagag cgcgaaagcg gatcgggttt actacgtgcc
     2101 gggcgagcac gatgtgttta ctgacgacgg aaaactctac ttcgagagat tcggcggcgg
     2161 gccgggatgg cggagtttcg acttcaacgg cgtgcatttc atcggattga tcaatgtgct
     2221 gaatctgaag cagggaaccg ggaccgggct gggcggcagt gggctcggac aattgggcca
     2281 ggaacagtta gcatggttgc agaaggacgt taagggtttg tctgccagca cgccgatcgt
     2341 ggtgtttgcg catattccgt tatggaccat ctatcccgat tggggttggg gcacggatga
     2401 gagtgggcag gcgttgtcgt atttgaagcg cttcggatcg gtgacggtgt tgaatggaca
     2461 cattcatcag gtgctgcaga aggttgaagg gaacattgcg tttcatacgg cgcgttcgac
     2521 tgcgtacccg caacccgcgc cgggcaccgc gccctcgccc gggccgatca aagatgtgtc
     2581 cgcggagaag ttgcgtagta cgttgggcgt gacggatgtt agttacatgg cgaaggttgg
     2641 accgttagcg gttgttgatg aagcgctagg ttgaggtatg ttaggcagga ttacaggatt
     2701 tgcaagatga acatgcttca tgtgtaaatc ctgaaatcct gtctaaaatc agagatgaaa
     2761 tttgcgaaga cggtttttct ggtggcgggg atttacg
//
LOCUS       DASSFQ010000064         8809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_923039, whole genome shotgun sequence.
ACCESSION   DASSFQ010000064 DASSFQ010000000
VERSION     DASSFQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923039"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..1046)
                     /locus_tag="VFH96_01940"
     CDS             complement(354..1046)
                     /locus_tag="VFH96_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET6972752.1"
                     /translation="MISKSWSRKSIATAVAVAVLSVYSMVVLAAPGAKASGELSVSGQ
                     VTVNGQKVISGGTVFTDSTISTAAQSSASVSISKVGRVELAPNSNLHLSFAENTITAM
                     LETGSAQVSTLSGTTVNLTTKDGTVVVDGTQPTTFTVSASRGRTHVSTLAGVAQFKAG
                     GAVKQVAAGESATAGTPNPQGDDDDDGLSGGALAVLLLAVGGAVAGVLYAAFHNNDLN
                     FGGSVTVVSPTK"
     gene            complement(1347..1847)
                     /locus_tag="VFH96_01945"
     CDS             complement(1347..1847)
                     /locus_tag="VFH96_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972753.1"
                     /translation="MATIKGRFSALLLLVFCALTPVNATPRTQISDDDEFGPVVRAYL
                     GYLKNEQEVVDDRASRREVSTTYYRHNSNRIRALRQMAIKLARETHNDYLPELEAVSA
                     GEMGLLFGSNAPAPARLKTGEVLRNTFRFLGPIRVVGEVFYLFARLDPYEQADLNEKS
                     SAVRRP"
     gene            2005..3204
                     /gene="coaBC"
                     /locus_tag="VFH96_01950"
     CDS             2005..3204
                     /gene="coaBC"
                     /locus_tag="VFH96_01950"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET6972754.1"
                     /translation="MSVKSVALGVCGGIAAYKAVEVLRGLQRAGCAVRVAMTKRACEF
                     VQPLTFRALSGSHVIVDDYAPDNPDPIAHITFSQTVDLLVVAPATANIIAKFANGVAD
                     DFLSSTYLACTAPVLIAPAMNTTMWEHPATRRNLRQLRADGVYFVEPDAGEMACGTIG
                     PGRLSEPERIVRAALEILTGSNRPQDLAGERFLITVGATREEIDPVRFISNRSSGKMG
                     FALAEAAVRRGAEVTVVAGVTTVDPPVGVRVLKALSADEMLKAVASEAGKASVFIGAA
                     AIADYRPAQKATQKIKKSNESITLTLERTPDVLSQVAAARANGMLVVGFAAETENVLG
                     NAKEKLRSKKLDAIVANDVSRADAGFDATTNAITIVTNDDAVELPLMSKRDAADKILD
                     VIVALRK"
     gene            3314..4072
                     /locus_tag="VFH96_01955"
     CDS             3314..4072
                     /locus_tag="VFH96_01955"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6972755.1"
                     /translation="MQEDVRNIIAETKKQLEHLRVLGVEGIQVTAEPVIAKPPVPSEN
                     IPAAKSITSLFGEMAPAPVKLTPSTETFEQIHAEIGDCTRCPLHLERTHVVHTEGNRK
                     ARLMFVGEAPGADEDIQSRPFVGRAGQLLTKIIEAIGMKREEVLIGNVNRCRPPGNRA
                     PTTEEATTCKPYLLREIAIVQPKVIVVLGNTAMKNLLDTREGITRLRGRFQDYKGIKV
                     MPTFHPAYLLRDPSKKRETWEDLKKVRDYLDSHP"
     gene            4078..6312
                     /gene="priA"
                     /locus_tag="VFH96_01960"
     CDS             4078..6312
                     /gene="priA"
                     /locus_tag="VFH96_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET6972756.1"
                     /translation="MANTSYAEVAVPLHVFQTFTYRLSPEQSVQAEVGARLVVPLGRN
                     LVTGYVVSLHDQLPAELGEVDVKDAHTLVDSEPVCNPEILQLARWVSDYYACPLGEVI
                     RAALPPGMTPKSRSGAKFAKPKLRRFVRLTSNGGEQKLTDTQRRVLATLQDGPMSLQA
                     LLTTADVSASTITSLEKKSLLEIYDDAIRRDPLAETLGIADAVHTLTAAQSSVLTQIE
                     QQMNSGAYSAFLLHGVTGSGKTEIYMRAMSRALSLGRSAMMLVPEIALTPVFSRRLRA
                     RFGDQVAIFHSSLQKGERFDEWTRVRNGDARVVIGTRSAVFAPVKNLGLIVVDEEHES
                     SYRQQESPYYNARDVAIVRAQKESATVVLGSATPSLESFHNARKGKYHFVALPERIGA
                     RPMAEAKIIDMRNVFVRHGKPRVFSDELLEAIRETRERGEQSIILLNRRGYSSFILCR
                     SCGETVQCPNCDVTLTYHRSERVIVCHYCNHREAVPRVCPSCGKKYIYYVGEGTEQLE
                     EMLKLLFPALRVARIDRDTTARRKVFEQTLIDFSAGRIDTLVGTQMLAKGHDFPNVTL
                     VGVVSVDAGLALPDFRSAERTFQLITQVAGRAGRGDRPGRVLIQTYHPYHYALRHSCA
                     QDYEAFYDEELRYRQNHSYPPFVALASLLVHGPDLGKVRNDSLELRKQLDLVNQERKC
                     RVLGPAPAPLSRLKGEHRFQLLIKSRSRKDLREVADAAMRAVAESGVNLRSINLEIDP
                     VSIM"
     gene            6331..7356
                     /gene="lpxD"
                     /locus_tag="VFH96_01965"
     CDS             6331..7356
                     /gene="lpxD"
                     /locus_tag="VFH96_01965"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET6972757.1"
                     /translation="MKISELAERTGARVEGLTDDIEITGAAGMDEAGDGHVTFLANPR
                     YTPRVNTTRASAIYLSEEAQTDRQIAILRVKDPYLAYTRALRIFNPEAELEPFVHPSA
                     VIDPTARVAKRVAIGACVVIGRNAFIGDDVRIYPNVTIYDDVTIGRGSVIHSGVAIRE
                     RSVIGERVVIHNNSVIGCDGFGYAKDEEKRWLKIPQAGRVVIEDDVEIGAATTIDRAS
                     VGESRVGRGSKLDNLVQIGHSCTVGEDTLLCAQVGLAGSSHIGNRVILAGQAGVAGHL
                     TIGDDVVLTAKSATSHDIPAGKIISGIPAFDNKDWLRSTAAFRRLGEMQRTLRELQRK
                     IATDNTD"
     gene            complement(7414..8163)
                     /locus_tag="VFH96_01970"
     CDS             complement(7414..8163)
                     /locus_tag="VFH96_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6972758.1"
                     /translation="MSLLLEGKRAFITGGTRGIGAAICEIFAREGADVAFSYNSRDDL
                     AAATSEKIEGYGRKAFNAKVSVIDRVGMKKLTRELVEKWGGIDILVNNAAINRGDNFA
                     TTTDRAWDEVIHTNVDSLFAVTKPIYKQMIRQRKGIILNITSIGAMRALPTAVHYATS
                     KAAMIGFTKCLSREAATFGVRVNAIAAGIFDTDLAQALPDRLLQMHDFWTSAGRLGRP
                     EELAEYAAFMVSERNSFMNGEIVIVDGGAIT"
     gene            complement(8264..>8809)
                     /locus_tag="VFH96_01975"
     CDS             complement(8264..>8809)
                     /locus_tag="VFH96_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972759.1"
                     /translation="LALAIACEQNVTPIDEQRLRRSFRYYRHRYRYKRNFGQVTWLLQ
                     AFSKWSEITGDRDFADLVFEIADWLLGYQQDKTGAFINDHQSDTPGYTTAVYLEGIAA
                     ALEITDKTRYDIYFNSLKRGFGFLDQLIIQERDRSILPNIDYALGGLRQGLHYSEVRT
                     DFVQHALSAILNLRSSAFIRS"
BASE COUNT         1939 a         2389 c         2579 g         1902 t
ORIGIN      
        1 agtcgcgcga tcttgtcttc atccacatcg acgcaggtga cgtcgacacc aaactcggca
       61 aagcatgctc cggtaacaag acctacataa ccggtaccaa ttacggcgat gtgcatttag
      121 ctactttctt ctctgcgtaa cctctgcgac ctctgcggtg aactaacgat taaatcgcag
      181 aggcgcagag gacgcagaga cagcgcagag aaaagaacaa tactctttac agtcaaatcc
      241 ttgcgggtca cgcgtgtgaa ttcacgtgac ccgcaaggtg aatttgcctg accgcagcgc
      301 tggttaccag tgccacagtg tttttattca acccgaacga actctttgcg agcttacttg
      361 gtcgggctta ctacggtaac gctgccgccg aagttaaggt cgttattgtg gaacgctgcg
      421 taaagcacgc ccgccactgc gccgccgaca gccagcagaa gcacggccaa cgcgccaccg
      481 ctcaaaccat cgtcatcgtc gtcaccttgt ggattcggtg ttccagcagt tgcactctca
      541 ccagccgcaa cctgcttgac tgctccacca gctttgaact gcgccacgcc cgcgagcgtc
      601 gacacatgag tccgtccacg tgaagcacta accgtaaacg tcgttggctg ggttccgtcg
      661 acaacaaccg tgccgtcttt ggtagtcaag ttaacggtgg ttcctgataa cgttgacact
      721 tgagcactac cagtctcgag cattgccgtg atggtgttct ccgcaaagct aaggtggaga
      781 ttgctgttgg gtgcgagttc gactctaccg actttgctga tgctgactga agcgctgctt
      841 tgagccgcgg tgctgattgt tgaatctgtg aaaaccgtgc caccggagat cactttttgg
      901 ccgttcacag tgacttgtcc ggagaccgat agctcgcctg acgccttggc gcccggcgcc
      961 gccaatacca ccattgagta aacactcaaa acggcaaccg ccacagcagt cgcaatggac
     1021 ttgcggctcc aagatttgct gatcattaac tttcctcccg taatgtgaaa cacaaaggtc
     1081 ttaattgcgt accgcgacgg ccgaacagtc gtgggaaaac aatgagatgt tagcaactct
     1141 gcgaggcccg ttaatgcatt gggattaggg cctcgaaacc actaacatgg cttttattac
     1201 actaatacaa gccctctgtc aacaatcatt tggcttaaca ttgccacaaa actgtgtcgg
     1261 accacgtggg ccacaaaagg acacaaatcg agtttggagt gcagcctttc agtcggctgc
     1321 ttttgacctg aactctaaac ggacactcag ggccggcgga cggccgaaga tttttcattc
     1381 aagtcggcct gctcgtacgg gtcgagccgc gcaaagaggt agaaaacctc accaactacc
     1441 cggattggtc ccagaaaccg gaaggtgttt cgcagcactt ccccggtctt caaacgcgcc
     1501 ggagccggtg catttgaccc gaaaagcagc cccatctcgc ccgcagaaac agcttcgagc
     1561 tcaggtaggt aatcgttgtg agtctcgcgc gccagcttga tcgccatctg gcgtaaagct
     1621 ctaatacggt tggaattatg ccggtaataa gtcgtgctta cttctcttct gctggctcga
     1681 tcgtctacca cttcctgctc gtttttcagg tagccgaggt atgcacgcac cacgggacca
     1741 aactcgtcgt cgtcggaaat ttgagttcgt ggagtggcgt tgacaggcgt gagcgcgcag
     1801 aaaacaagca acagcaaagc ggagaatctt cccttgatag ttgccatcgt tttgctcctc
     1861 gcgggaggca atcgtctatt tttcttgcgg ccgcccgatt ataccaacac gtaagtcaac
     1921 taaattgcgc gcgtctttcg gcaatgttac attgatcttc gatttccgtt aagcagcatg
     1981 tcttcttcag atcctcaagc tcctttgtcg gttaagagcg tcgcgctggg tgtctgtggc
     2041 ggtatcgcgg cgtacaaggc agtcgaagtt ctccgcggtt tgcagcgggc gggctgtgcc
     2101 gtcagagtcg ccatgacgaa gcgggcctgc gagtttgtgc agcccctcac gttccgcgcg
     2161 ctttccggta gtcacgtaat tgtcgatgat tacgcgcctg ataatccgga cccgatcgcg
     2221 cacatcacgt tttctcagac ggtcgatctg ttggttgtcg cgcctgcgac cgcgaatatc
     2281 atcgctaagt tcgccaatgg agtcgccgac gactttctca gctcgactta cctcgcctgc
     2341 accgctccgg ttttgattgc gcccgcaatg aacacgacga tgtgggaaca tccggcgacg
     2401 cggcgaaacc ttcgccagct tcgagccgat ggggtttatt ttgtcgaacc tgatgcgggc
     2461 gagatggcgt gcgggacgat tggtccgggg cgtttgagtg agcccgagcg cattgttcgc
     2521 gctgcactcg agattctcac aggatcaaac cgtccgcagg acctggcggg cgagcgcttt
     2581 ttgataactg tgggggcgac gcgtgaagag atcgatccgg tgcgatttat ttcgaataga
     2641 tcgtcgggca agatgggctt cgcactcgct gaagcagcgg tgagacgtgg ggcggaggtg
     2701 actgtcgttg caggtgtcac gacggtcgat ccaccagtag gggtgcgggt cttgaaagcg
     2761 ttgtccgccg atgagatgtt gaaagcagtt gcgagtgaag ccggcaaagc gtcggtgttc
     2821 attggtgcgg cggcaatcgc agactatcgt ccggcgcaga aggctacgca gaagataaaa
     2881 aaatcgaatg agtcgataac attgacgttg gagcgcacgc ccgatgtgtt gtcgcaggtt
     2941 gcggcagcac gcgcgaatgg aatgcttgtg gtgggatttg cggcagagac ggaaaacgtt
     3001 ttgggtaatg cgaaagagaa actgcgcagt aagaagctcg atgcgattgt tgcgaatgat
     3061 gtgagtcgtg cggatgcggg tttcgatgcg acgacgaatg cgatcacaat cgtcaccaat
     3121 gatgatgcag ttgaattacc attaatgtcg aagcgtgatg ccgcggataa aatactcgat
     3181 gttatagtgg cgcttcgtaa ataactggac gcttcgtcaa taactgtagg ggcggcactc
     3241 cgtggccgcc cgttcgcgac aacattttgg tgatcaagcc gcgggcggcc acagagggcc
     3301 gcccctacaa aagatgcaag aagacgttcg caatattatc gctgagacaa agaagcagct
     3361 cgagcacttg cgtgtgcttg gggttgaggg aattcaggtg actgccgagc cggtgatcgc
     3421 aaagccacct gttccgagtg agaatattcc cgccgcgaag tcgataacat ccttgtttgg
     3481 cgagatggcg ccggcgccgg tgaaactcac gccatcaacc gaaacattcg aacagatcca
     3541 cgccgaaatc ggtgactgca cgcgttgccc gctgcatctc gagcgcacgc acgtggtcca
     3601 cactgaagga aaccggaaag cgcgattgat gtttgtcggc gaagcgcctg gcgctgatga
     3661 agacatccag tcacgcccgt ttgtaggtcg cgccgggcag ttgctcacca agatcatcga
     3721 agcaatcggg atgaagcgtg aagaagttct gatcggaaac gtcaaccgct gccgcccacc
     3781 aggcaatcgc gcgcccacaa cggaagaagc aacgacgtgc aaaccgtacc tgctgcgcga
     3841 aatcgcgatc gtgcaaccaa aagtgatcgt cgtgctcggc aacaccgcga tgaaaaacct
     3901 gctggacact cgcgaaggca tcacacgact gcgcgggcgc ttccaggatt acaagggaat
     3961 caaagtgatg cccaccttcc atcccgcgta cctgctccgc gatccttcga agaaacgcga
     4021 gacgtgggaa gacttgaaga aagtccgcga ttatcttgat agccatccgt gaaatctgtg
     4081 gctaatacct catacgctga agtcgcagtt ccgctgcatg tctttcaaac gttcacttac
     4141 cgcctgagcc ccgaacagag tgttcaggca gaggtgggcg cgcgcctcgt cgtaccgctc
     4201 ggccgcaacc tcgtcaccgg ctacgtcgtc agtcttcacg atcaacttcc ggctgagctt
     4261 ggcgaagttg atgtaaaaga cgcgcacaca ctggtcgact ccgaaccagt ctgcaatccc
     4321 gagatcctgc aactcgcacg ctgggtttcg gattactacg cgtgcccact cggcgaagtg
     4381 atcagagccg cgctaccgcc gggcatgact ccgaagagtc gctcgggtgc gaagtttgcg
     4441 aaaccgaaac tgcgccgctt cgttcgctta acatcgaatg gaggggaaca gaagctgact
     4501 gatacgcaac ggcgggtgct tgcaacgctc caggatggtc caatgtcgtt gcaggcttta
     4561 ctcaccacgg ccgacgtaag tgcctccacg atcacgtctc tcgaaaagaa atcgctgctg
     4621 gagatctacg acgacgcgat tcgacgcgat ccgcttgctg agactctcgg catcgcagac
     4681 gcagtacaca ccctcactgc ggcacaatca tcggttctca cacagatcga acagcagatg
     4741 aactccggtg cttattcagc gtttctgctg cacggcgtta ccggcagcgg caagacggag
     4801 atctacatgc gcgccatgag ccgggcgctg agcctcggta ggtctgcgat gatgctcgtg
     4861 ccggagatcg cactcacgcc agtcttctcg cgtcgtctgc gcgcacgctt tggcgatcag
     4921 gtagcaattt ttcattcatc gcttcagaag ggtgagcgat ttgacgagtg gacgcgcgtc
     4981 cgaaacggtg atgcacgggt cgtgatcgga acgcgttcgg ccgtgttcgc gcctgtgaaa
     5041 aacctcggac tgatcgttgt cgacgaagaa cacgaatcat cgtaccgcca gcaggaatcg
     5101 ccgtactaca acgctcgcga cgtcgcgatc gtgcgcgcgc aaaaggaatc ggctacggtc
     5161 gtgctcggct cagcaacgcc ctcgctcgaa tcttttcaca atgcacgcaa aggcaagtat
     5221 cactttgtcg cgctgccgga acgcatcggc gcacggccga tggccgaggc gaagatcatc
     5281 gacatgcgca acgtgtttgt gcgccatgga aagccgcgcg tgttttccga cgagctactc
     5341 gaagcgattc gcgagacgcg cgaacgtggc gagcaatcga tcatcctgct caaccgccgc
     5401 gggtattcga gttttatact ttgtcgctcg tgtggcgaga ccgttcagtg tccaaactgc
     5461 gacgttacgc tcacttacca tcgcagcgag cgcgtgatcg tctgtcacta ctgcaatcat
     5521 cgcgaagcag tgccgcgcgt ttgcccttca tgcggaaaga aatacatcta ctacgtgggc
     5581 gaaggaacgg agcaactcga ggagatgctc aagcttctgt tccctgccct gcgggtcgca
     5641 cgaatagatc gtgataccac cgcgcggcgg aaggtgtttg agcagacgtt gattgacttt
     5701 agcgcgggcc gcatcgacac gcttgtcggc acgcagatgc tggcgaaggg ccatgatttt
     5761 ccaaatgtga cgttggtcgg cgtggtttcg gttgatgcgg gactggcatt acccgatttc
     5821 cgatctgcgg agcgaacgtt tcaactcatc acgcaggttg cgggacgggc tggtcgtggt
     5881 gatcgaccgg ggcgagtctt gatccagacc tatcatccat accactatgc tctacgacac
     5941 tcgtgcgcgc aggattatga agcgttttac gacgaggagc ttcgctaccg gcagaaccat
     6001 tcgtatccac cgttcgtggc gctcgcgagt ttgctggtcc atgggccgga tttgggtaag
     6061 gtgaggaatg atagtcttga gttgcggaaa caactcgatc tggttaatca ggagcggaag
     6121 tgtcgcgtgc ttggtccggc gccggcgccg ttgtcgcgct tgaaaggcga gcatcgtttt
     6181 cagttgttaa tcaagtcgcg cagccggaaa gatctgcgcg aggtggcgga tgcagcgatg
     6241 cgagcggtcg cggagagtgg agttaacctg cgttcaatca atttggagat cgatccggtg
     6301 tcgatcatgt agagacagga tgtaaacaag atgaaaattt cagagctggc ggaaagaacg
     6361 ggcgcgcgcg tcgaaggatt aacggacgat atagaaatca ctggcgcggc ggggatggat
     6421 gaggccggtg acgggcatgt gacgtttctc gccaacccgc gctacacgcc gcgtgtgaat
     6481 accacgcgcg cgtcggcgat ctacctgagc gaagaggcgc agaccgatcg acagatcgcg
     6541 atcctgcggg tgaaggatcc ttatcttgcc tacacgcgcg cgctgcgcat cttcaatccg
     6601 gaagccgagc tcgagccgtt tgtgcatccg tccgccgtta tcgatccgac tgcgcgcgtg
     6661 gcgaaacgcg tcgcgatcgg cgcgtgcgtg gtgattggac ggaacgcctt catcggcgac
     6721 gacgttcgca tttatccgaa cgtgacgatc tacgacgacg tcacgatcgg tagaggctcg
     6781 gtcatacatt ccggcgtcgc gattcgtgaa cgcagcgtga tcggcgagcg cgttgtgatc
     6841 cacaacaact ccgtgatcgg ctgcgacggt tttggttacg cgaaagacga ggagaaacgc
     6901 tggctcaaga ttccgcaggc agggcgagtc gtgatcgaag acgatgttga gatcggggcg
     6961 gcgactacga tcgatcgggc gtcggttgga gagagccggg tcgggcgtgg atcgaagctg
     7021 gataatctcg tgcagatcgg tcactcgtgt actgtgggtg aggatacgtt gctgtgcgcg
     7081 caggtcggat tagcgggcag ctcacacatc ggcaatcgcg tgatactcgc gggccaggcc
     7141 ggcgtcgcgg gacatctgac gatcggcgat gacgttgtgc tgacagcgaa gagcgcgacg
     7201 agtcacgata ttccggcagg aaaaatcatc tcggggattc cggcgtttga taataaagat
     7261 tggctcagat cgacggcggc tttccggaga ttgggtgaga tgcaacggac gcttcgtgag
     7321 ctacaacgaa aaatagccac agataacacg gattgaaaaa cggattttcg ctgatctgtg
     7381 taaatcgcgt attgagatcc gtctaatccg tggctaagta atagcgccac catcgacgat
     7441 cacgatctcg ccgttcataa aactattccg ctcactcacc atgaaggccg cgtactccgc
     7501 aagctcttca gggcgcccga ggcgtccggc tgaggtccag aaatcgtgca tctgcaacag
     7561 tcgatcgggc agcgcctgcg cgagatccgt gtcgaagatt cctgccgcaa tcgcattcac
     7621 tcgcacgcca aacgtcgccg cttcgcgtga caggcacttc gtgaagccga tcatcgctgc
     7681 tttcgaagtc gcgtaatgga cggcggtcgg aagtgcgcgc atggcgccga tcgaagtgat
     7741 gttgaggatg attccctttc gttgccggat catctgtttg tagatcggct ttgtaaccgc
     7801 aaacagacta tcgacgttcg tgtgaatcac ttcatcccac gcgcgatcgg tggtggtggc
     7861 gaagttgtcg ccgcgattga tggccgcgtt gttgacgagg atgtcgatcc cgccccactt
     7921 ctcgaccagc tcgcgcgtca gtttcttcat gcccacgcga tcgataacgg agaccttggc
     7981 attgaacgcc ttgcggccgt agccctcgat cttttcgctc gtcgcggccg ccagatcgtc
     8041 acgcgagttg taactaaacg cgacatctgc gccctcgcgg gcaaagattt cgcaaatggc
     8101 cgcaccgatg ccacgcgttc cgccggttat gaacgcgcgc tttccctcaa gcaacagact
     8161 cacttagcta tgtttctcct agaagtttga cggggattag tttaaacgcc gcgcccgatt
     8221 aaaccgtgaa aacgggcgcc cagtcaattc ggggcgacgc tctttagctg cggatgaacg
     8281 cggatgaacg cagatttaga atggctgaca gggcgtgttg gacgaaatcg gtgcggactt
     8341 cgctgtaatg aagtccctgt cgcagaccgc cgagagcgta gtcgatgttg ggaagtattg
     8401 agcgatcgcg ctcctggatg atgagttggt ccaggaagcc gaagccgcgc tttagcgagt
     8461 tgaaataaat gtcgtagcga gttttatcgg tgatctccag ggcggcagcg atgccttcga
     8521 ggtagacggc ggtggtgtag cccggcgtgt cggattgatg gtcgttgatg aaagcgccgg
     8581 tcttgtcctg ttgataaccc aggagccagt cggcgatctc aaagacgagg tcggcaaaat
     8641 cacgatcacc ggttatttcc gaccacttgc tgaaggcttg cagcagccac gtgacctgac
     8701 cgaagtttcg tttgtagcga tagcgatgac ggtagtaccg aaagctccgg cgcaagcgtt
     8761 gttcgtcgat cggggtcacg ttttgctcgc acgcgatcgc gagcgcgag
//
LOCUS       DASSFQ010000065        18364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_180918, whole genome shotgun sequence.
ACCESSION   DASSFQ010000065 DASSFQ010000000
VERSION     DASSFQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18364
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180918"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VFH96_01980"
     CDS             complement(<1..1098)
                     /locus_tag="VFH96_01980"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET6972760.1"
                     /translation="MRTFRAPGRVNLIGEHTDYNDGFVMPAALDFFTWVRLAPLAKRK
                     LHVFSENFNEEVEVDLDDPNLAARGHWSDYPIGVAVVIERAGYRLRGARLRIRGEVPI
                     GSGLSSSAAIEVATACALAANSGLDIDRRELALLCQRAENEFVGARVGIMDQFVSLFG
                     QAQHALLLDCRSLEFKLLPLPDNVRLIICNTMVKHELASSAYNERRAQCEAGVGLLAQ
                     FYPNVKALRDVTIEQLEQHRSEMPEVVYRRCRHVVTENARVIAASAALAQQDLDRFGK
                     LMAASHVSLRDDYEVSSDELDLMVELADKIDGVYGARMTGGGFGGSTVNLVREENADE
                     FRARVAREYEIVTKLKPEIYIRSAGNGAEEVA"
     gene            complement(1190..2035)
                     /locus_tag="VFH96_01985"
     CDS             complement(1190..2035)
                     /locus_tag="VFH96_01985"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6972761.1"
                     /translation="MRLAAIIVLLSLSVSAQAQSALNDMVKTEQAFSQMAQDKGIHDA
                     FMTYIADDGLLFRPGAVNGKKWMSEHPVPSSDKNPVLVWQPAYAGMAVSGDMGFTTGP
                     WEAKADINDEKAERHGHFVTVWKKQADGTWKFVVDLGISHPQSGGPQTLWTPAETPKK
                     EFKPVDVPIALETLLERDRNYAAAVLSRGFGKSYAAYAARTVRLYRANNLPFIGREAS
                     IEQLSKTTGEVKWIPIGGDVSSAGDLGYTHGTYEAGDERGSYVRIWKKQNGLWRIVMD
                     VTNAH"
     gene            complement(2061..3557)
                     /locus_tag="VFH96_01990"
     CDS             complement(2061..3557)
                     /locus_tag="VFH96_01990"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6972762.1"
                     /translation="MALKLSKISKSFGPTVALDGVDLELREGEVHALIGENGAGKSTL
                     MNVIAGSLRPDQGTMEIRGKPYAPSNPLDARTHGIALIHQELSLCPHLSVAENVLMGI
                     ESSHLGWLDRDQLIARTNDVLKTFHHADIRPEKRVGDLSVAARQIVEISRALAANARI
                     ILMDEPTSSLQREDVVHLFTLIRKLSAEGISVIYISHFLEEVRQISDRFTVLRDGRSV
                     AGGEIANVSDDELIAKMVGRAQQNLFPLRSHTAAQIETILEVQNLAAPPLLKSASFEL
                     RRGEILGIAGLMGSGRTPLLRTIFGLERPESGTIKVNKKSLRPGSSTPAMRLIEGLGY
                     LSEDRKGDGLTLNQSIADNVTVTRFNSCSRWGWLDLGAQRKQTEKLVNALKIRTQGVQ
                     QEVGSLSGGNQQKVIVARLLHQDADVLLLDEPTRGIDIGSKAQVYETIAACAAQNKAV
                     LMVSSYLPELFGMCDRLAVMSRGRLSEVRPINEWTPESVLQTAIGAAD"
     gene            complement(3574..6948)
                     /locus_tag="VFH96_01995"
     CDS             complement(3574..6948)
                     /locus_tag="VFH96_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6972763.1"
                     /translation="MRALTKLLAVLLASSLAVSAQTDLTKDPTLYVVAYAHLDTQWRW
                     EYPRVINDYIPKTLHDNFALIDKYPHYIFNFSGANRYRMMREYWPADYARLKQYVAAG
                     RWFPAGSSMEEGDVNSPSAESIFRQILYGNRFFQREFGKTSAEYMLPDCFGFPASLPS
                     ILAHAGLKGFSTQKLTWNSAAPAGGPGSIEETPRGIPFNVGYWVGPDGRGIVAALNPG
                     SYGGQVREDISKSPAIAGNNTVDWPRRVERNGKASGLFTDYHYYGTGDTGGSPREDSV
                     RMMEAIVTRNNALVEAQAVVGDGPLKVVSATAEQMFLNIRPEHIARLPRYEGDLLLTD
                     HSAGSITSQAYQKRWNRKNELLADAAEKASVGAAWLGTRVYPQERLNNAWTLVMGGQF
                     HDILPGTATPKAFNFSWNDDVIAMNQFAGVLTSATEGIASALDTRGVGNPIVVYNPLN
                     IEREDVVEVEVARPGGTWSVFGPDGRKVASQVVGGKLLFPAKVSSIGYAVYDIRFMPY
                     GAAHAHQSADSELKVSPRSLENQRYRIALNVNGDVSSIFDKQLNRELLAAPIRLAIKN
                     DTPAQWPAWNMDWTDQQKEPRVYVRGNVRVKVVENGPVRVAVEVERETEGSKFNQTIC
                     LSTGDAGNRVEFSDVIEWKTANANLKATFPLTAANGKATYNWDIGTIERGNNDERKFE
                     VPSHQWFDLTDRSGTFGVTILSDCKYGSDKPDDKTLRLTLLRTPGIAARAGYADQSTQ
                     DWGRHEIVYGLAAHAGDYRRAQTDWHAQRLNQPLIAFKSSKHPGTLGKTFSILHTNSS
                     RVRVLALKKAEDSDELIVRIVELDGREQPDVHIAFAAPVIAARELNSQEFPIGKAAID
                     KGELATSLGRFQPRTFAVKLAPRSSKLTPPQSTPITLPYDLATADRDGERMKAGFDGE
                     GYALPGEMLPHDLPYGGITFHLGPPDKANALVARGQEISLPARVQRLYVLAASVGGDQ
                     DVAFRVGSEEMKVTIQDWSGFIGQWDNRQWKETEVRLQPRTPSPDIPADVAALLQRSR
                     TRLDPYGEMLGITPGFIKRTPVGWFASHRHTPAGTNEAYAYSYLFVYTFDVPVNARTL
                     TLPNNDRVRIMAITGSNERSTLKPAHPLYDTLEQNP"
     gene            7005..7283
                     /locus_tag="VFH96_02000"
     CDS             7005..7283
                     /locus_tag="VFH96_02000"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HET6972764.1"
                     /translation="MTISFSDRVTIPDDVLISQLQEESVILNLDSERYYGLDDVGTRF
                     LSVLTTSDSIETAYDQLRHEYNVDPQVLRDDLLSLVENLLDQGLLIRT"
     gene            7296..8819
                     /locus_tag="VFH96_02005"
     CDS             7296..8819
                     /locus_tag="VFH96_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HET6972765.1"
                     /translation="MANELTITANARLDARDDLNRSDAELILDAYEKWGDDCVKHLLG
                     DFAFTIWDGRRQRYFCARDHFGVKPFYFTHINNEFAFSSSLNELRPLVSNTLNEIAVG
                     DYLLFGVNQDLSTTIFKDIQRLKPGHTLTVDNGSIAVRPYWTPEPSTEVRYRDPLSYV
                     EHFSELLARAVKDRVTEDRVAISMSGGLDSTSLAALAHEQGKEVHGFAVVYDSLIPDE
                     ERHYSSLAAQHLGIPVTHLSADRYSLFDEQVPGDMDQPEPFQLSALTGQFNDLLRLAA
                     DFSTVALTGYDGDAFMNVPQRSRLGGVKKRIKQLIPGKRAATEGRPYSWLDEAFAKRT
                     HLVERWERPSIEQASPLNPIWTALFEGYDRSSTKLNLEVRHPFMDVRLVEFLSSIPVR
                     PWRVNKQILRAAMNDKLPSAVVTRRKTPLAGDPALQLVRRGSVRCLDSFEVSPQLRTF
                     VNLNRRRSVADEETSDGLWANLRIFALNYWLTNSQPGVRRTSQTQVTTVAATKTSNA"
     gene            8844..9014
                     /locus_tag="VFH96_02010"
     CDS             8844..9014
                     /locus_tag="VFH96_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972766.1"
                     /translation="MERKPNPTTQELETKKPYHKPEVISYGNIREITRNVGSKGVADG
                     GGGAGAGPKTGS"
     gene            9023..9988
                     /locus_tag="VFH96_02015"
     CDS             9023..9988
                     /locus_tag="VFH96_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972767.1"
                     /translation="MHYRCSIYGLGVISNRAIPGVPPSTIASEDVRISFGSLPAWLDE
                     VTALQVETSYVADYKSECGEPVLRVFRVRDGEYYRFCYADETEFVIDKVGTEVWAAWS
                     DPLTLADTATYLLGPVMGFVMLLRGVVCLHASAVAVGDEAIALLGPAGSGKSTTAAAF
                     AERGYSILAEDVVTLDDRGNQFLVRPGYGLIRLWPAAVKALYGSETHLPKLTPNWDKC
                     YLDLNDNFQREPLRLAAIYHLSERRHDATAPFVQPLDRAEGLMSLVANTYATKLMDKH
                     MRAREFELLTRVVSNVPVRRVTPHSDVTLIPELCTRIIADFKSGF"
     gene            10010..10954
                     /locus_tag="VFH96_02020"
     CDS             10010..10954
                     /locus_tag="VFH96_02020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6972768.1"
                     /translation="MYSLHFYGQMLADAPRMDAYAAALRHTVNRDSVVMDLGCGPGVF
                     ALLACKLGARRVYAVEPDNVIGLAREAAAPNGFADRIEFFEKLSTEITLPEPATIIVS
                     DLRGVLPWFEQNISSIIDARKRLLAPRGVLIPRRDILWAAVVEAPEQYAELVSPWANQ
                     FDLDLSAATRCITNNWRKTHIKPEHFLAEPVCWNTIDYEEVDSRDVRAEISWLAARNG
                     TAHGFAIWFDSELVDDIGFSNHPAAPRMIYGQGFFPFSQPVEVAAGDRIELRLAADFV
                     QDNYVWRWDTDFIDQERIKASFKQSTFYGMRFSTAQLK"
     gene            10954..12789
                     /locus_tag="VFH96_02025"
     CDS             10954..12789
                     /locus_tag="VFH96_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972769.1"
                     /translation="MFSSVKILRSKLSKALAQLPYLPRALKLVWEVARPWTTAWIALL
                     IVQGLLPAATVYLTKLVVDGVVAALRNGNSRPVLLPLLLLGGVLLLMEVVRNAINWVR
                     TVQAELLQDHITSLIHEKSVAVDLAFYELSDYYDHLHRARAEARYRPVALLGNLGALL
                     QNSITLVAMGAILIPLGPWLALALLLSTLPAFYVVVHYALAEYQWRQRTTADDRRAWY
                     YDWVMTTAEAAAEIRLFGLGKHFQSLHKTLRTRLRSERLHLTRRQGFAELAASLIALV
                     IMGAALALMVLKASHGLVTLGELALIYAAFNQGQGLMRTLLENAGQLYGNSLFLGNLF
                     EFLALQPNNCRGGPPWPPVLEIQRAITFNNISFSYPDASSKALDNFTMTIPTGNIVAI
                     VGPNGAGKSTLLKLLCRFYDPDSGSIAIDGKDLKDFPTDDLRRLITVLFQQPFHYNST
                     VRDNILYGDLKLDATDAEIKAALRAAGAEEIVSRLPEKEQTLLGRWFAGGTELSVGEW
                     QRIALARAFLRQAPIIILDEPTSALDPWAEADWLERFRQLAFGRTSIIITHRFTTAMH
                     ADMIHVMDRGRIVESGNHQRLINQNGLYAQSWSRQMTSTAYAKSV"
     gene            12805..13959
                     /locus_tag="VFH96_02030"
     CDS             12805..13959
                     /locus_tag="VFH96_02030"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6972770.1"
                     /translation="MANSLILYIARRDTDPDHLRSLIKHDLDWDYLLTVAFAHGLLPL
                     LHKHLLTDLVPAQILSRLKRESVANSQNVLHLTGKQLRVYKLFKENNVPVALFKGPLL
                     SQMAYGEMSLRQAGDIDLLINRNDFSRARLLLESLGYEMTPRLTPAQLSSHLNNHCEI
                     QFMRDEWLTVVDLHWDLAPRSFVFGVKPEDVMSRLQSVSLAGATIETFGPEDLLLYQA
                     MHGAKHLWRRLEWITCLAESLRATTTINWDTLVDRAARARATRILALGLRLVEEFSDA
                     PVPSEVFVSVDPDKIMQRMATQIRAQLFTTFGPAESTETNLYNLRIMDRKRDALVSVW
                     RSIFVPTLPDWQSLALPASLHSLYYAYRPLRLSKVYSASLWRKLTGVRDV"
     gene            13952..14722
                     /locus_tag="VFH96_02035"
     CDS             13952..14722
                     /locus_tag="VFH96_02035"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HET6972771.1"
                     /translation="MYKPPLKWAGGKRWLVSHLEPIWESNKQRRYVEPFCGGLAVVLG
                     LEPKQALLNDVNPHLINFYRHVQNGLRMRVRMRNDEELFYRHRERFNRLIRRGKAHTE
                     EGAQLFYYLNRTCFNGLCRFNKGGDFNVPFGTYKAINYARDFSSYAAAFRQWEFSNVD
                     VETLPLRAGDFVYADPPYDVEFTTYSAGGFSWDDQVRTAKFLAAHRGPVVISNQATPR
                     IIELYEGFGFQLSYLDAPRRISCTGDRTAAREVLAIKP"
     gene            14782..16116
                     /locus_tag="VFH96_02040"
     CDS             14782..16116
                     /locus_tag="VFH96_02040"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HET6972772.1"
                     /translation="MSISTWLPDLIYVNGRFEPNRALVCESGKILKISNDNGGIRLKN
                     RALLPGMVNAHSHAFQRVIRGRTEHRSHHTTDSFWTWREQMYAAANRLGPEEIYNASR
                     LAFLEMALTGITAVGEFHYIHHAPDGSTYDDPNLLAREVIRAAHDVGIRIALLRVAYA
                     RSGFGLEANPQQIRFIEQSPDTYLKHLEQLLDAPELKSDMAWAGVAPHSVRAVPLDYL
                     KTIVAFADERELPVHMHVAEQPAEVSACIEEYGRSPVALLDTEALLSKRFTAVHAIHV
                     TPKAIASMENAGAIVCACPTTERNLGDGVVPVDAYFDAGVRVSLGTDSQVQIDLLEDA
                     RELEYHLRLQKTARNVLAPVDDDSSSALARRLFECATINGAESIGFNGGKLEPGAPAD
                     FFTVDLSDLSIAGCAVEDLLANIVFSMARTTIRDVVVGGKQIVNNGKHGLDG"
     gene            16113..17216
                     /gene="argE"
                     /locus_tag="VFH96_02045"
     CDS             16113..17216
                     /gene="argE"
                     /locus_tag="VFH96_02045"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HET6972773.1"
                     /translation="MIKQILADLVRIDSVSTRSNAEITAYLERRCEALGLITRRFPYT
                     DEHGLEKINLIALTHDVQEVELALVGHTDTVPYDPNWSEATNLTERDGKLYGRGACDT
                     KGFIAAALTAVENVKPGKPLALIFTADEEVGLLGAKKLAQAKPLRVRYSIVGEPTSLK
                     PIRAGKGYSLAEVIVKGREAHSAYPALGASAVFRAARLINRLESIATQLKQDQYAAFD
                     PPFTTLNIGLIHGGSAKNVLAGECRFTLEWRPIPTQSSEHLLDLFNAAIAEEMKSDPD
                     FQCVVDASRQDTGFETSPDSPLVQLLENTTGNKSGTVAFGTEAAQMMLFGSEAVVIGP
                     GDIREAHRTGEFVPVSELERCAEVLRQSIHKLC"
     gene            17254..18354
                     /locus_tag="VFH96_02050"
     CDS             17254..18354
                     /locus_tag="VFH96_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972774.1"
                     /translation="MLRINKALLVVLLLWSCVPLSAQDSKPLTNQEFVKLLYQLQRSP
                     DTRDDLIEQIRKRGIGFPLTDGMRSLVATKSGNDPLLRRTLEEAERRRVNPTASTLPP
                     ENEGHDLLERTRNVTLAAANAMPDFLVKQIIKRSVAYGNTSNWILRDNLTIAVGYRAN
                     VGEEYKVLTVNGMAPPPGEDAEASKDFSKNKYVPKGASSSGVEYISAVADVFKPESKA
                     EFRMVDTDLVQSRRTVVYEYVVKKDNSQLTLGLADTGANALVGSRGRVWIDRELDRVL
                     RFEQIATEIPPDFPITAASSLIDYDWISINERKYLLPTHSEILLTTINPQMVVQSRND
                     IRFRNYQKFGAELKVVDEVGEDDEPTPPPKKP"
BASE COUNT         3999 a         5347 c         4935 g         4083 t
ORIGIN      
        1 ggccacttct tctgctccat ttccagcact acgaatgtaa atctcgggtt tgagtttggt
       61 cactatctca tattccctcg ctacgcgcgc acgaaactcg tcagcattct cttcccttac
      121 cagattaact gtcgatccac caaacccacc accagtcatg cgcgcgccat aaactccgtc
      181 gatcttatcg gcgagctcga ccatcagatc cagctcatca gaactgactt cataatcatc
      241 acgcaagctc acgtgtgacg cggccatcaa ctttccgaag cgatccagat cctgctgcgc
      301 cagtgccgcg ctcgccgcaa tcactcgcgc attctccgtc acaacgtgac ggcaacgtcg
      361 ataaaccact tccggcattt cactgcggtg ctgttcgagc tgctcgatcg tcacatcacg
      421 cagcgctttc acgttcggat aaaactgcgc caacaatccg acaccggctt cacactgcgc
      481 gcgtcgctca ttgtaagcgc tcgatgccag ctcgtgcttc accatcgtgt tgcaaattat
      541 caaccgaacg ttgtcgggca gcggcaacag cttgaattcg agtgaacgac aatcgagcag
      601 cagcgcgtgc tgtgcctgcc caaacaacga aacaaactgg tccatgatac caacccgcgc
      661 gcccacaaac tcgttttccg cacgctggca cagtaacgcg agctcgcgcc tgtcgatatc
      721 gaggccggaa tttgccgcga gagcacacgc tgtcgcgact tcgatcgcag ccgacgagct
      781 gagtcccgaa ccgatcggta cttcgccacg aattcgtaag cgtgcgccgc gcagacgata
      841 acccgcgcgc tcaatcacaa ccgcgacgcc aatcggataa tcgctccagt ggccacgtgc
      901 ggcgagattt ggatcgtcga gatcgacctc gacttcttcg ttgaagtttt ctgaaaagac
      961 gtgcagtttg cgttttgcga gcggcgcgag acgcacccac gtgaagaaat cgagcgcggc
     1021 gggcatgacg aagccgtcgt tgtaatcggt gtgttcaccg atgagattca ctcgacccgg
     1081 agccctgaac gtacgcactc gtcgcctcaa gaattaatta gccgcggatt aacgcggatg
     1141 aacgcagatc agcgtttatc cgcgttcatc tgcggctaaa tattcttagc taatgcgcat
     1201 tcgtgacgtc catcacgatg cgccacagac cattttgttt cttccagatg cggacgtagc
     1261 tgccgcgctc gtccccagct tcgtacgtgc cgtgtgtgta tccgagatcg ccggcgctcg
     1321 atacatcacc acctatcggt atccacttaa cctcgccggt cgtcttcgag agttgctcga
     1381 tggaggcttc gcggccgatg aacggaagat tattcgcgcg ataaagacgg accgtacgcg
     1441 cagcgtatgc ggcatagctc ttaccaaacc cgcgactcaa cacagccgca gcatagtttc
     1501 gatcgcgttc gaggagcgtc tcgagtgcaa tcggaacgtc aacaggcttg aattctttct
     1561 taggcgtttc agccggtgtc cacaacgtct gtggtccacc tgactgcgga tgcgagatgc
     1621 cgagatcgac gacgaacttc cacgtgccgt ccgcctgctt cttccagacc gtcacgaaat
     1681 gaccgtggcg ctccgccttc tcatcgttga tgtcagcctt agcttcccat ggtccagtgg
     1741 tgaaacccat gtcgcccgac accgccatgc cggcatacgc aggctgccat acgagcaccg
     1801 ggttcttgtc tgaagacggc accggatgct cgctcatcca cttcttgcca ttcaccgcgc
     1861 cgggccggaa cagcagtcca tcatccgcga tgtacgtcat gaacgcatca tgtatgccct
     1921 tatcctgggc catctgcgag aacgcctgct cagtcttcac catgtcgttc aatgccgact
     1981 gcgcctgcgc acttacactc aaagacaata gaactatgat tgctgctaat ctcatctgtg
     2041 tagatccccc cttcatccgt ctaatccgcg gccccgattg ctgtttgtaa cacagactcc
     2101 ggggtccatt cattgatcgg ccgcacttcc gatagtctcc cacgactcat caccgccagt
     2161 cgatcgcaca tcccaaacaa ctccggcaag taagagctca ccatcaacac cgccttgttc
     2221 tgcgccgcac acgcagcgat cgtttcgtaa acctgcgctt tgctaccaat gtcgatgcca
     2281 cgcgtcggct cgtcaagcag aagcacatcc gcatcctggt gcagcaaacg cgccacgatc
     2341 accttctgct gattgccgcc cgaaagcgat ccaacctcct gctgcacacc ctgcgttctg
     2401 atcttcagcg cgttcacaag cttctccgtc tgcttgcgtt gcgcgcccag atcgagccag
     2461 ccccaacgcg agcacgaatt aaatctcgtg accgtaacgt tgtcggcgat cgactggttc
     2521 aacgtcaagc cgtcgccttt gcgatcttcg ctgagatatc caagaccctc gattagacgc
     2581 atcgccggcg tgctgcttcc gggccgcaaa ctctttttat ttactttgat cgtaccactt
     2641 tcaggacgct cgagtccgaa gatcgtgcgc aacagcggcg tgcggcctga gcccatcaaa
     2701 ccggcgatac cgagtatctc gccgcgacgc aactcaaacg atgcactctt caataatgga
     2761 ggcgctgcaa gattttggac ttccagtatc gtttcgattt gcgctgctgt gtgtgaacgt
     2821 agagggaaga gattttgttg cgcgcgaccg accatcttcg cgatcaattc gtcatcgctg
     2881 acgtttgcga tctctccgcc tgctacgctg cgaccatcac gcagcactgt gaagcgatcc
     2941 gaaatctggc gtacttcttc aagaaaatga ctgatgtaga tcaccgagat gccttcagcg
     3001 ctcaacttac ggatcaacgt aaagagatgg accacgtctt cgcgttgcaa actgctcgta
     3061 ggctcgtcca tgagaatgat gcgagcgttt gcagcgagcg cgcgcgaaat ctcgacgatc
     3121 tggcgcgcgg caacagagag atcgccgaca cgtttttccg gacgaatgtc tgcgtgatga
     3181 aatgttttca gaacgtcgtt ggtgcgcgca atgagctggt cacgatcgag ccagccgagg
     3241 tgtgacgact cgatgcccat caagacgttt tcggcaacgc tcaggtgcgg gcagagggac
     3301 agctcctggt gaatcagtgc gatgccatgc gtgcgtgcgt cgagcgggtt tgagggagcg
     3361 tacggtttgc cgcggatctc catcgtgcct tgatcgggac gcaacgagcc ggcgatgacg
     3421 ttcatcaggg tgctcttgcc tgcgccgttc tcgccgatca gtgcgtgtac ttcgccttcg
     3481 cgcagctcga gatcgacccc gtcaagcgcc accgttggtc caaacgactt ggaaattttt
     3541 gatagtttca gggccacgga ttacacggat tttttacgga ttttgttcca gggtgtcgta
     3601 gagtgggtga gcgggcttca acgtcgagcg ctcatttgat ccagtgatgg ccatgattcg
     3661 tacgcgatcg ttgtttggca gcgtcagggt tcgcgcgttc accggtacgt cgaatgtgta
     3721 cacgaacaag tatgaataag cgtacgcttc gttggtgccc gccggtgtgt ggcgatgcga
     3781 tgcgaaccaa cccacaggcg tgcgtttgat gaaaccgggt gtgatgccca gcatctcgcc
     3841 gtacggatcc aatctcgttc gcgatctttg caacagcgcg gcaacgtcag cgggtatgtc
     3901 cggcgacgga gtgcgcggct gtaaacgcac ttccgtttcc ttccactggc gattgtccca
     3961 ctgtccgatg aagccgctcc aatcctgaat cgtcactttc atctcttcac tgccaacacg
     4021 aaaagcgacg tcctgatcgc cgccaaccga cgctgcgagc acgtacaacc gctgcaccct
     4081 cgcgggtaat gaaatctcct gtccgcgcgc gacgagcgca tttgctttgt ccggcggacc
     4141 gagatgaaaa gttataccac cgtatggaag gtcatgcgga agcatttcac ccggcagcgc
     4201 gtaaccttcg ccgtcgaagc cagccttcat acgctcgcca tctcgatccg cggttgcgag
     4261 atcgtatggc aaagtgatag gcgtcgattg cggtggagtg agtttcgatg atcgtggcgc
     4321 gagtttcacc gcaaacgtgc gcggttgaaa tcgtccgaga ctcgttgcga gttcgccttt
     4381 atcgattgca gctttgccga tcggaaattc ctgactgtta agttcacgcg ctgcgatcac
     4441 cggcgcggcg aaggcgatat gcacatcagg ctgttcgcgt ccatccagct cgacgatccg
     4501 cacgatcagc tcgtcgctat cttcagcttt cttcagcgca aggacacgaa cgcgactact
     4561 gtttgtatgc aggatcgaaa acgtcttgcc gagcgttcct ggatgcttcg agctcttaaa
     4621 tgcgatcagc ggctggttca aacgctgcgc gtgccagtca gtctgtgcac gacgataatc
     4681 gccggcgtgt gctgcgagac cgtaaacgat ctcgtgccgt ccccaatcct gtgttgattg
     4741 atccgcatag cctgcccgcg cagctatccc cggcgtacgc aggagagtca aacgaagtgt
     4801 tttgtcgtca ggcttatcag aaccgtactt gcagtcggaa aggatcgtga cgccaaacgt
     4861 accgctgcga tccgttaaat cgaaccactg gtgcgatggc acttcaaact tgcgctcgtc
     4921 gttgttgcca cgctcgatcg tcccgatgtc ccagttgtaa gtcgccttcc catttgccgc
     4981 agtgagtgga aacgtcgctt tgagatttgc gttcgcagtc ttccactcga tgacgtcgct
     5041 gaactcgaca cgattgccgg catctcccgt cgataggcaa atagtttgat taaacttcga
     5101 accttcagtc tcgcgctcaa cttccactgc tacacgcact ggaccatttt caaccacctt
     5161 cactctgaca tttcccctta cataaacacg cggctctttc tgctgatcgg tccaatccat
     5221 attccatgcg ggccactgcg cgggcgtatc gttcttgatc gcaagacgta ttggtgcagc
     5281 gagcagctcg cggttgagtt gcttgtcaaa aatactcgac acatctccat taacgttcag
     5341 cgcgatccgg taacgctgat tttcaagcga gcgcggcgac accttcaact ccgaatcagc
     5401 actctggtga gcatgcgcag cgccgtacgg cataaatcgt atgtcataca ctgcgtaccc
     5461 gatagacgaa actttcgccg gaaacaacag cttgccaccc acgacttgcg atgcaacttt
     5521 gcgcccatca ggaccaaaca cactccatgt cccaccaggt ctcgcaacct ctacttcaac
     5581 aacgtcttcg cgttcgatgt tcagcggatt gtatacaacg attgggtttc cgacgccgcg
     5641 cgtgtccaat gccgacgcaa ttccttcggt cgcgctcgtc aacacacccg caaactggtt
     5701 catcgcgatc acatcgtcgt tccacgaaaa gttaaacgcc ttcggcgtcg cggtgcccgg
     5761 caaaatgtcg tgaaactggc cacccatcac gagcgtccac gcgttgttca agcgctcctg
     5821 cggatacaca cgcgtcccca gccaggccgc gccgaccgaa gctttctctg ccgcatcggc
     5881 aagcagctcg ttcttacggt tccaacgttt ctgatacgcc tgcgaagtga tcgaacccgc
     5941 ggagtgatcc gtcaacaaca gatcaccttc atagcgcggc agtcgagcaa tgtgttccgg
     6001 acgaatgttg agaaacatct gctccgcggt cgccgagacg actttcagtg gtccatcacc
     6061 gactacggcc tgcgcttcga cgagcgcatt gttacgggtg acgatcgctt ccatcatgcg
     6121 cactgaatct tcgcgcggcg agccgccggt atcgccagtg ccgtagtagt gatagtcagt
     6181 gaagagcccg ctcgcctttc cgtttcgttc gacgcgtcgc ggccaatcga ccgtgttgtt
     6241 tccggcgatc gcagggctct tgctgatgtc ttctctaact tgtccgccgt aactgccggg
     6301 attgagcgcc gcgacgatgc cacgcccgtc tggcccaacc cagtaaccta cgttgaatgg
     6361 aatgccgcgc ggcgtttcct caatcgagcc ggggccgccc gcgggcgctg ccgaattcca
     6421 cgtgagcttt tgggtcgaga agcctttcaa tccggcgtgc gcgagaatgc tcggcagcga
     6481 tgccggaaaa ccaaaacagt caggcagcat atactcggcg ctcgtcttgc caaattcgcg
     6541 ttggaaaaag cgattgccgt agagtatctg gcgaaagatc gactccgcag acggagagtt
     6601 gacgtcgcct tcttccatcg acgagcctgc cggaaaccat cgacccgcgg cgacgtactg
     6661 cttcaggcgc gcgtaatcgg cgggccagta ttcacgcatc atgcggtaac gattcgcgcc
     6721 gctgaagttg aaaatgtagt gcggatactt gtcgatgagc gcgaagttgt cgtgcagcgt
     6781 cttgggaatg tagtcgttga tgacgcgcgg atactcccag cgccactgcg tgtcgagatg
     6841 cgcgtacgcg acgacgtaca gcgtcggatc tttggtgaga tcagtttgtg ctgagaccgc
     6901 cagagaactt gcgagtaaaa cagcaagaag cttggttaac gctcgcatgt gcgtgggctc
     6961 cttttgccgc gacagaataa cagagcgtgt tagaatgcgc agccatgaca atttcattct
     7021 cggatcgcgt gacgatccct gacgacgtcc tgatctcgca gctccaggaa gagtcggtaa
     7081 ttctaaatct cgacagcgaa cgctactacg gtctcgatga cgtcggcacg cgctttttat
     7141 cggtcctcac cacttcagac tcgatcgaga ctgcctacga ccaattgcgc cacgaataca
     7201 acgtcgatcc acaagtccta cgcgacgatc tgctctcgct cgtcgagaac ctcctcgatc
     7261 agggcctctt aatccgtact taatccgtca aatccgtggc taatgaatta acgatcacag
     7321 ccaacgcccg ccttgatgct cgcgacgatt taaaccgatc tgatgctgaa ttgattctcg
     7381 acgcgtacga gaaatggggc gatgattgcg tcaaacacct gctcggcgac tttgctttca
     7441 ccatctggga cggccgtcga caacgctact tctgcgcgcg cgatcatttc ggtgtgaagc
     7501 cgttttactt cacgcacatt aataacgaat tcgcgtttag cagcagcctt aatgaactcc
     7561 ggccactcgt ctcaaacaca ctcaacgaga tcgctgtcgg cgactacctg ctcttcggag
     7621 taaaccagga tctatcgacg acgatcttca aagacattca gcgactaaag cccggccaca
     7681 cactcacagt cgacaacggc tcgatagcag tgcggcctta ctggacacca gaaccgtcaa
     7741 ccgaagtgcg ctatcgagat ccgttatcgt acgtcgaaca cttctccgag ctgttagcgc
     7801 gggccgtgaa ggatcgggtg accgaagatc gtgttgcaat ctcgatgagt ggcgggcttg
     7861 actcgacgag tctggcggca ctcgcacacg aacaaggcaa agaggttcat ggtttcgcgg
     7921 ttgtttacga cagcctgatt ccggatgaag aacgtcatta ctcaagtctc gccgcgcaac
     7981 atctcggcat tccggtaacg catttgagtg ccgatcgcta ttcgctattt gatgaacagg
     8041 tgcccggcga catggatcag cccgagcctt ttcagttgtc cgcactaaca ggtcagttca
     8101 acgatctgct gagactggct gcggatttca gcacggtcgc gcttacgggt tatgacggtg
     8161 atgcgtttat gaacgtgccg caaaggtcgc gtctcggagg agtcaagaaa aggatcaagc
     8221 aactgattcc cgggaaacgg gcggccacgg agggccgccc ctacagttgg ttggacgagg
     8281 cgtttgcgaa acggacgcat ctggttgaga ggtgggaaag accgtcgatc gagcaagcga
     8341 gtcctttaaa tccgatctgg acggcgctct tcgaaggtta tgatcgcagt tctactaaat
     8401 tgaatctcga ggtgcgtcat ccgtttatgg atgtgcggct tgttgaattt ttgtcatcga
     8461 ttccggtgag gccgtggcgt gttaataaac aaattctgcg cgctgcgatg aacgataaac
     8521 taccgtccgc cgtagtcact cgacgaaaaa caccactcgc tggcgatccg gctcttcaat
     8581 tggtacggcg cggtagtgta agatgcctcg atagttttga ggtcagtccg cagctcagga
     8641 cttttgttaa cctgaatcgt cgacgatcag tggcggatga agagacatcc gacggcctct
     8701 gggccaatct ccgaatcttc gcgttgaact actggcttac gaactcccag cccggtgttc
     8761 gacgaacgtc gcaaacccag gtgactacag tcgcggcaac aaagacttca aacgcttaaa
     8821 ctggatgaag atggagatca tgcatggaac gtaaacccaa ccccaccacc caggaactcg
     8881 aaacgaagaa gccatatcac aagccggaag tcattagtta cggaaacatt cgcgagatca
     8941 caaggaacgt tggttcaaag ggcgttgcgg atggcggcgg cggcgccgga gccggaccca
     9001 agaccggaag ctaatccctg tcatgcatta ccggtgctcg atctacggtc ttggagttat
     9061 ctccaacaga gcaattcccg gcgttcctcc ttcaacaata gcatcggaag acgttcgcat
     9121 atccttcggc tcacttcctg catggctcga tgaagtaacg gccttacagg ttgagacctc
     9181 ctacgtcgca gattacaaaa gtgaatgcgg tgaacctgtc ttgcgcgtgt ttcgcgtgcg
     9241 tgacggcgag tattacaggt tctgctacgc tgacgagacg gagtttgtga tcgacaaagt
     9301 agggaccgaa gtctgggctg cgtggagcga tcctttgacg ctcgcagaca cagccaccta
     9361 cctgcttggt ccagtgatgg gatttgtgat gctgctgcgc ggcgttgttt gtttacacgc
     9421 gagtgcggtt gctgttggag acgaagcgat cgcgttgctc ggtccggcag gatcgggtaa
     9481 gtcgactaca gcggctgcgt ttgcggaacg cggttacagc attcttgccg aagatgtcgt
     9541 gacgttggat gatcgcggca atcaatttct cgtgcggccg ggttacggtt taattcgttt
     9601 gtggccggcg gcggtcaagg cgctttatgg atccgagacg cacttgccaa agctcacgcc
     9661 aaactgggac aagtgctacc tcgatttaaa cgataacttt cagcgggagc cattgcggct
     9721 cgcggcgatc tatcatctca gcgagcgacg tcacgacgcc accgcgcctt tcgtgcagcc
     9781 gctcgatcgc gcggaagggt tgatgtcgtt ggtggcgaac acgtacgcga cgaagttgat
     9841 ggacaagcac atgcgcgcgc gtgagtttga attgttgacg cgtgtcgtga gcaacgtgcc
     9901 cgtgcgacga gtaacaccac actcagacgt aacgctcatt ccggaactgt gtacacggat
     9961 tatcgcggat tttaaatccg gattttaaat ccgtgttatc cgtggctaaa tgtatagtct
    10021 tcacttctac ggccagatgc tcgctgatgc tccgcgtatg gatgcgtacg cggcggcgct
    10081 acgtcataca gttaatcgcg attcggttgt gatggatctc ggttgtggcc cgggcgtgtt
    10141 tgctctcctc gcgtgcaagc tgggggcacg gcgtgtttac gctgtcgaac cggataacgt
    10201 gatcggtctg gcgcgtgaag ctgctgctcc aaacggcttc gccgatcgaa tcgagttttt
    10261 cgagaagctc tcgactgaga tcacgttacc cgagcccgcg accattatcg tttcagattt
    10321 gcgcggtgta ctgccgtggt tcgaacaaaa tatttcttcg atcattgacg cgcgcaagag
    10381 actgctggcg cctcgcggtg tgctgatccc acgccgggat attctatggg ccgcagttgt
    10441 tgaggcgccc gagcaatacg cggagctcgt tagcccgtgg gcaaaccagt tcgacctcga
    10501 cctatccgcc gctacacgtt gcatcaccaa caactggcgt aagactcaca tcaaaccaga
    10561 acactttttg gcggaacccg tgtgttggaa cacgatcgat tacgaggaag ttgacagtcg
    10621 cgacgtgcgc gctgagatct cgtggctcgc agcacgaaat ggcacggcgc acggattcgc
    10681 catctggttt gattcagagc tggtggacga tatcggcttt tcaaatcatc cggcggcgcc
    10741 ccgaatgatc tacggccagg gcttctttcc tttttcgcaa ccggtggaag tcgcggcagg
    10801 cgatcgcatt gagttgcggc tggcggcgga ttttgtgcaa gacaattacg tttggcgttg
    10861 ggacactgat ttcatcgatc aggaacgtat aaaagccagc ttcaagcagt cgacgttcta
    10921 cggcatgcgg ttctcgacgg cgcaacttaa ataatgttca gctcagtcaa aattctccgc
    10981 tcgaaactaa gcaaggcgct cgcgcaactg ccttacctgc cgcgcgcgct gaagcttgtt
    11041 tgggaagtgg cgcgaccgtg gaccactgcc tggatcgcgc tgctgatcgt gcagggactt
    11101 ctgccggcgg cgactgttta tctcacgaag cttgtggtcg atggtgtggt cgctgcactg
    11161 cgcaatggaa attcgcggcc cgtgctgttg ccattgttgc tgcttggcgg cgtgttgttg
    11221 ctgatggaag tcgtgcgcaa cgcgatcaac tgggtgcgca cggtgcaggc ggagctgtta
    11281 caggaccaca tcacgtcgct gattcacgag aagtcagtag cagtcgatct cgctttttac
    11341 gagctgtctg attattacga ccacctgcat cgcgcgcgtg ctgaagcacg gtatcgcccg
    11401 gtagcgctgc tcggcaatct cggcgcgttg ctgcaaaaca gcatcacgct cgtcgcgatg
    11461 ggcgcgatcc tgattccgct tggaccatgg ctggcgctcg cactcctgct cagcacgttg
    11521 ccggcgtttt atgtcgtcgt tcattacgca ctcgctgagt atcaatggcg acaacgcacc
    11581 accgccgacg atcgacgtgc gtggtattac gactgggtca tgaccacggc tgaagcggcc
    11641 gcggagatcc ggctcttcgg tctcggcaag cacttccaat cactgcacaa aacattacga
    11701 acgcggttgc gcagcgagcg tcttcactta acgcgtcgac aaggcttcgc ggaattagca
    11761 gcaagcctga tcgctctcgt gattatgggc gccgctctcg ctttgatggt attgaaagcc
    11821 tcgcacggcc tcgtcacgct cggcgagctc gcgctgatct atgctgcctt caatcaaggc
    11881 cagggcctga tgcgcacgct cctcgagaat gccggccagc tctacggcaa cagcctcttc
    11941 ctcggcaacc tcttcgagtt tcttgcgctc caacccaaca actgtagggg tggccctccg
    12001 tggccacccg ttcttgaaat tcaacgcgcc atcactttca ataacatctc cttctcctat
    12061 cccgacgcgt cgagcaaagc cctcgacaac ttcaccatga cgatcccaac cggaaacatc
    12121 gtcgcgattg tcggcccgaa cggcgccggc aagagtacgc tgctgaaatt gttgtgtcgc
    12181 ttctacgatc ccgactcagg ctcgatcgca atcgacggca aagatctcaa agacttccca
    12241 accgacgatc tgcgacgact aatcaccgtc ttgtttcaac agccatttca ctacaacagc
    12301 accgtgcgcg ataacatcct ctacggcgat ctcaaactcg acgcgactga cgctgagatc
    12361 aaagctgcgc ttcgcgccgc aggcgccgaa gaaattgttt cgcgtctccc ggaaaaagaa
    12421 caaacccttc tcggccggtg gttcgccggc ggaacagagc tcagcgtcgg cgagtggcag
    12481 cgcatcgcac tcgcgcgcgc gtttctgcga caggctccga ttatcattct cgacgagcca
    12541 accagcgcgc tcgatccgtg ggccgaagca gactggctcg aacgcttccg ccaactcgcc
    12601 ttcggccgta cttcgatcat catcacgcac cgcttcacca ccgcgatgca cgccgacatg
    12661 atccacgtaa tggaccgcgg tcgaatcgtc gaatcaggca accatcaacg actaatcaat
    12721 cagaacggtt tgtacgccca atcctggtca cgtcaaatga cgtcaactgc ttacgccaaa
    12781 tccgtataaa aatccgtgca atccgtggct aacagtctga tcctctacat cgcgcgtcgc
    12841 gatacggatc ccgatcactt acgttctctc ataaaacacg acctggattg ggactatctg
    12901 cttaccgtcg ctttcgccca cggactgctc ccactcctgc acaaacacct cctcactgat
    12961 ctcgttcctg ctcaaatcct ctcgcgcctg aagcgcgaat ccgtcgcgaa ttcacagaac
    13021 gtactacatc tcaccggcaa acagcttcgc gtctacaagc tcttcaaaga gaacaacgtt
    13081 cccgttgcgc tctttaaagg cccgttgctc tcccaaatgg cttacggcga aatgtctttg
    13141 cgccaggccg gcgatatcga tctgctgatc aaccgcaacg acttctcccg agctcgactg
    13201 ctgctcgaat cgctgggcta cgaaatgacg ccgcgactaa ctcccgcgca gctatcgtca
    13261 cacctgaaca accactgcga gatccagttc atgcgcgacg agtggctcac ggttgtcgat
    13321 ctccactggg atctcgcgcc gcgcagcttt gtcttcgggg ttaaaccgga agacgtaatg
    13381 tcgcgactgc aatcagtctc gctcgcgggc gccactatcg agactttcgg acctgaggat
    13441 ctcctgctgt accaggcgat gcacggcgcg aaacatttgt ggcgacgttt ggaatggatc
    13501 acgtgtctcg ccgagtcgtt acgcgccaca acaacgatta actgggacac gctcgtcgat
    13561 cgtgcagcaa gagcacgcgc aacacgcatc ctcgcgcttg ggttgcgact cgttgaagag
    13621 ttttcagacg cgccggttcc atccgaagtc ttcgtatcag tcgatccaga caaaatcatg
    13681 caacgcatgg cgacgcagat tcgggcgcag ctcttcacca cgtttggacc agccgaatca
    13741 accgaaacca atctctacaa cctgcgcatc atggaccgca aacgcgatgc gctcgtctcc
    13801 gtctggcgct cgatctttgt cccaaccttg ccggactggc agtcactggc gttgcccgca
    13861 tcgctgcatt cgctctacta cgcgtatcgc ccgctgcgac tctcaaaagt ctacagcgcc
    13921 tcgctctggc gcaaactcac aggtgttcgc gatgtataag ccgcccttga agtgggccgg
    13981 cggcaagcgt tggctcgtct cgcatctcga accgatctgg gagtcaaaca agcaacgtcg
    14041 atacgtcgag ccgttttgtg gcgggcttgc ggtggtgctg ggtctcgaac caaagcaggc
    14101 actgctcaac gacgtcaatc cgcacctgat caatttttat cgtcatgtgc aaaatggttt
    14161 gcggatgcgc gtgcgcatgc gaaacgatga ggagcttttc tatcgacatc gcgagcgctt
    14221 caatcgtttg atccggcgcg gcaaggcgca cacggaggaa ggcgcgcagc tgttctacta
    14281 tctcaaccgc acgtgcttca atggtctgtg tcgtttcaac aaaggcggcg atttcaacgt
    14341 gcctttcggg acttacaaag cgatcaacta cgcgcgcgat ttctcaagtt atgcggcggc
    14401 gtttcggcag tgggagttta gtaatgttga tgtggagacg cttccgttgc gggcgggaga
    14461 ttttgtgtat gccgatccgc cgtatgatgt agagttcacg acgtacagtg cgggcggatt
    14521 ttcatgggat gatcaggtgc gcaccgcgaa gtttctggcg gcgcatcgtg ggccggtggt
    14581 gatatcgaac caagcgacgc cgcggattat cgagttgtat gaagggttcg ggtttcagtt
    14641 gagttatctg gatgcgccgc gaaggattag ttgtactgga gatagaactg ctgcgcgcga
    14701 ggtgttagcg ataaaacctt agacaggatt gacaagattt tttcaagatg aacatgtcaa
    14761 tcctgtcgaa tcttgtaaat catgtctata tctacctggc tgccggatct gatttatgta
    14821 aacgggcgct tcgagcctaa tcgcgcgctc gtctgcgagt ctggaaaaat tctcaagatc
    14881 tccaacgata atgggggcat ccgcctgaag aatcgcgccc tcctccccgg gatggtcaac
    14941 gcgcattcgc acgcattcca acgagtcatc cgcgggcgca ctgagcaccg ttcacatcac
    15001 acaacagaca gcttttggac gtggcgcgag cagatgtatg ccgccgcgaa ccgcctcggc
    15061 cctgaagaga tctacaacgc gtcacgcctc gcgtttctcg agatggcgct gacgggcatc
    15121 actgctgtcg gtgagtttca ctacattcat cacgcgcctg acggctcgac ttacgacgat
    15181 ccaaacctgc tcgcgcgtga agtgattcgc gccgctcacg acgtcggtat tcgcatcgct
    15241 ctcctgcgtg tcgcttacgc acgatcggga ttcggtctcg aagccaaccc acagcagatc
    15301 cgttttatcg agcaatctcc cgacacttat ctcaagcatc tcgaacaact actcgacgcg
    15361 cccgagttaa aaagcgacat ggcctgggcc ggcgtggcgc cgcatagtgt tcgcgccgtg
    15421 ccgcttgatt acctcaagac gatcgtggcg ttcgcagacg agcgtgagct tccggttcac
    15481 atgcacgtcg cggaacagcc ggcagaagtg agcgcgtgta ttgaagaata cggtcgctcg
    15541 cccgtcgcgt tgctcgatac cgaagctctg ctgagcaaac gcttcaccgc agttcatgcg
    15601 attcacgtaa cgccgaaagc gatcgcgtca atggaaaacg caggcgctat cgtgtgcgcg
    15661 tgcccgacga cggaaagaaa cctcggcgac ggtgttgtgc cggttgatgc gtatttcgat
    15721 gcgggcgtgc gcgtatcgct cgggactgac agccaagtcc agatcgatct gttagaagac
    15781 gcgcgcgagc tcgagtatca cctgcggtta cagaagactg cgcggaatgt gctcgcgccg
    15841 gttgacgacg actcgagctc agcactcgcg cgaaggttgt tcgaatgcgc gacaatcaac
    15901 ggcgcggaga gtatcggctt caacggaggg aaactcgagc cgggagcgcc ggctgatttc
    15961 ttcactgtcg atctcagtga tctctcgatt gcgggatgcg cagttgaaga tctactcgca
    16021 aacattgtgt tttcaatggc gcgtacgaca attcgcgacg tggtcgtcgg tggcaaacaa
    16081 atcgttaaca acgggaaaca cggattagac ggatgattaa acagattctc gcggatttag
    16141 tacggattga ttcggtctcg acgcgatcga atgcggagat cacagcttat ctcgagcgac
    16201 ggtgcgaggc gttgggatta atcacgcggc gctttccgta cacggatgag cacggactcg
    16261 aaaagattaa cttgattgcg ttgactcacg atgtgcagga agttgagctc gctctcgtgg
    16321 gccacacgga tactgttcct tacgatccta actggtccga agcgacaaac ctcaccgaac
    16381 gtgacggcaa actctacggc cgcggcgcgt gcgatacgaa gggtttcatc gcggccgcgt
    16441 tgactgccgt agagaacgtc aaaccaggca agccgctcgc actaatattt accgccgacg
    16501 aggaggtcgg attactcggc gctaagaagt tggcccaggc gaagccgttg cgcgtgcgtt
    16561 acagcatcgt cggcgaacca acttcgctga aacctattcg cgccggcaaa ggctactcgc
    16621 tcgcggaagt gatcgtgaaa ggacgtgaag cacacagcgc ttatcctgct ctcggcgcgt
    16681 cagccgtgtt tcgcgccgcg cgactcatta atcgactcga atccatcgcc acacaactca
    16741 aacaagacca gtacgccgca ttcgatccac ctttcacaac tctcaacatc ggcttgattc
    16801 atggaggctc agcgaagaat gtgttggctg gcgagtgccg gttcacactc gaatggcgac
    16861 caataccaac acaatcttca gaacacctgc tcgatctttt caacgcggcg atcgccgagg
    16921 agatgaagtc tgatccggat tttcagtgtg ttgtcgacgc gagccgccag gacacaggct
    16981 tcgagacatc gcctgactca ccactcgttc agctgctgga gaatacaaca ggcaacaaat
    17041 caggcaccgt agccttcgga acggaagccg cacagatgat gttgttcggc agtgaagcgg
    17101 tggtgatcgg gccgggagac attcgcgaag cacatcgaac cggggagttt gtgcccgtga
    17161 gcgagcttga gcgttgtgcc gaggttctgc gacaatccat ccacaaactt tgttgaggca
    17221 accgccagta gtaaaagtcc atcggaaggt tcaatgctcc gcattaataa agctctttta
    17281 gtagttctcc tcttgtggtc gtgtgttccg ctctcagcgc aggattcaaa gccactcacc
    17341 aatcaggaat tcgtcaagct gctctatcaa ctccaacgca gccctgacac acgcgacgat
    17401 ctcatcgagc agatcaggaa gcgcggcatc ggttttccgc tcactgacgg catgcgctcg
    17461 ctcgtcgcta cgaagagtgg taacgatcca ctactgcgac gcacgctcga agaagcggag
    17521 cgccgccgcg taaacccaac cgcgtccaca ctcccacctg aaaacgaagg acacgacctc
    17581 ctcgaacgca cacgcaacgt cacgctcgct gcggctaacg caatgcccga cttcctcgtg
    17641 aagcagatca tcaagcgctc tgtcgcttac ggcaacacgt ccaactggat tttgcgtgac
    17701 aatttgacga tcgctgtcgg ttatcgcgcg aatgtcggcg aggagtacaa agtcctgacg
    17761 gtgaatggaa tggcgccgcc gcccggtgag gatgccgaag cgagcaagga tttcagtaag
    17821 aacaagtacg tgccgaaggg cgcatcgtcg agcggcgtgg aatatatctc ggcggtcgcg
    17881 gatgttttca agcctgagtc caaagcagaa tttcggatgg tcgatacgga tctggttcag
    17941 agcagacgca cggtcgttta tgaatacgtg gtcaagaaag ataactcaca actgactctg
    18001 gggcttgccg atactggggc gaacgcgctg gtcggatcac gcggtcgcgt ctggatcgat
    18061 cgcgagctgg atcgcgtgct gcgtttcgag cagatcgcga ctgagattcc gcctgacttt
    18121 ccgatcaccg ccgccagcag tttgatcgac tacgattgga tcagcatcaa tgagcgtaag
    18181 tatctgctgc cgacgcattc agaaatcctt ctgacaacga tcaacccgca gatggtggtt
    18241 cagagtcgca acgatattcg cttccggaac tatcagaagt ttggagcgga gctgaaggtg
    18301 gttgacgaag tcggtgaaga cgacgagccc acacccccac caaagaaacc ataaatatta
    18361 gccg
//
LOCUS       DASSFQ010000066         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1517450, whole genome shotgun sequence.
ACCESSION   DASSFQ010000066 DASSFQ010000000
VERSION     DASSFQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517450"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2520)
                     /locus_tag="VFH96_02055"
     CDS             complement(<1..>2520)
                     /locus_tag="VFH96_02055"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HET6972775.1"
                     /translation="SKAISISSTYDHRIIQGAESGAFLARVHEFLIGKHDFYKNIFLN
                     LGIPHAPMRWNVDRNPFFLTEDRVREQTLKEARVIELINAYRVRGHLIADTDPLHALP
                     ILYHPELDIENYGLTIWDLDRVFITDGLANRESATLRDILDILQRAYCGKVGIEYRHI
                     QSKEEKQWIRERIRQQFVEPQPIPVDVKKRLLWKLISAEQFERFLHTKYLGQKRFSLE
                     GCETIIPVLDQLIDGAAVRGVEAITFGMAHRGRLNVLANVIGGLCERIFAAFEGSVHP
                     EFPADEGDVKYHQGAKGERVMADGRKVQLSLSPNPSHLEAVDPVVEGMVRATQDEMMG
                     QRGANREQVIDLALPVLLHGDAAFAGQGIVMETLNLAGLKGYRTGGTIHIIINNQIGF
                     TTSPEAGRSTIYSTDVARMTQLPIFHINGDDPEAAYRVLQIALDYRQEYNKDVVLDVI
                     GFRRLGHNETDEPSYTQPLMYSRVKAHPGVRTVYAKRLIKEGVITEEELNNLIAERVR
                     GYEAALANAKREVAEEPKHPYARVDEMDGSEVIPTGVPAETIRSIAHKIAVVPEGFHV
                     NPKMVGQLARRAKMGDGSLPLDWAFAEAIAYGSLVLEGNRVRLSGQDSGRGTFSQRHA
                     VLYDTQTGQTWAPLAEFRSDKNPDARFDVYDSSLSEEGVLGFEYGYTVVAQHALVLWE
                     AQFGDFGNGAQTIIDQYIAASEDKWKQTSRLVMLLPHGYEGQGPEHSSARLERYLQLC
                     AENNLQVCYPTTPAQYFHLLRRQVRPGMERPLIVMTPKSLLRLPAATSSIDDLESGGF
                     RPLIDDAEATDRNAVTRVVLCSGKVYYDLAEARNKTENRNVA"
BASE COUNT          526 a          720 c          687 g          587 t
ORIGIN      
        1 cgcgacgttc cgattctcag tcttgtttcg tgcttcggcg agatcgtaat aaaccttgcc
       61 actgcaaagc acgactcgcg tcactgcgtt gcggtcggtg gcctccgcat cgtcaatcag
      121 cggtcgaaaa ccgccggact cgagatcgtc gatgctggaa gtagcagccg gcaagcgcag
      181 caggctcttc ggtgtcatca cgatcagcgg gcgctccatg cctggcctga cttgtcgtcg
      241 cagcagatga aagtactgcg ccggtgtcgt tggataacaa acctggagat tgttctccgc
      301 acagagttgc aggtatcgct caagtcgcgc gctcgaatgt tccggtccct gaccttcgta
      361 accatgtggc agcaacatca ccagtcgtga agtctgtttc cacttgtctt cagaagcggc
      421 gatgtactga tcgataatcg tttgcgcgcc gtttccaaaa tcaccaaact gcgcttccca
      481 cagcacgagt gcgtgttgcg caactacggt gtagccatat tcaaatccga gcacgccttc
      541 ttccgacagc gagctatcgt aaacatcaaa acgcgcgtca ggatttttgt ccgaacgaaa
      601 ttcggctaac ggcgcccacg tctgtccggt ttgcgtgtcg taaagaacag cgtgacgctg
      661 gctgaacgtg ccacgtcctg aatcctgtcc actcaaacgc acgcgattgc cttcgagcac
      721 gagcgagccg taagcgattg cttcggcgaa tgcccaatca agcggcaaac tgccgtcgcc
      781 catctttgcg cgtcgcgcga gttggcccac catcttcgga ttgacgtgga agccctcagg
      841 cacgactgca atcttgtgcg cgatgcttct gatcgtttcg gcgggaactc cggttggaat
      901 cacttccgaa ccatccatct cgtccacacg cgcatacgga tgtttcggtt cttcagcgac
      961 ttctcgtttc gcgtttgcaa gggctgcttc ataaccgcgc acgcgctccg cgatcaaatt
     1021 gttgagttct tcttcggtga tgacgccttc tttgatcagc cgctttgcgt agacagtgcg
     1081 cacacccgga tgcgctttga cgcgcgagta catcagcggt tgcgtatagc tgggctcgtc
     1141 ggtttcgttg tgaccaagcc ggcggaaacc gatcacgtcg agcacgacgt ctttgttgta
     1201 ttcctgacga taatccagcg cgatctgaag cacgcgatag gcagcttcgg gatcgtcgcc
     1261 gttgatgtga aagatcggta gctgagtcat gcgggcgacg tcagtcgagt agatcgtcga
     1321 gcggccggct tcaggagaag tggtgaagcc gatttgattg ttgatgatga tgtgaatcgt
     1381 cccgccggtg cgatagccct tcaaaccggc gaggttgagg gtttccatca caataccctg
     1441 gccggcaaaa gctgcgtcgc catgcagcag caccgggaga gcgagatcga taacctgctc
     1501 gcggtttgcg ccgcgctggc ccatcatctc atcctgtgtc gcgcgcacca ttccttccac
     1561 caccggatcg acggcttcga ggtggctcgg attcggcgag agcgacagtt gcaccttgcg
     1621 cccgtccgcc ataacgcgtt caccttttgc gccctggtga tatttcacgt cgccttcgtc
     1681 agccggaaac tccggatgca ccgatccttc gaacgccgcg aaaattcttt cacaaagacc
     1741 gccaatcacg ttcgcaagca cattcaaacg cccgcgatgg gccataccga aggtgattgc
     1801 ttcgacaccg cgaactgcag cgccgtcgat caattggtcg agcaccggga tgattgtttc
     1861 gcagccttcg agtgaaaacc gtttttgtcc gagatacttc gtgtgcagaa aacgttcaaa
     1921 ctgttccgcc gatatcagct tccaaagcag acgtttcttc acgtcaaccg ggatcggctg
     1981 tggttcgaca aattgctggc ggatccgttc gcgaatccac tgcttttcct ccttgctctg
     2041 aatgtggcga tattcgatgc cgactttgcc gcagtacgca cgttgcagga tgtcgagaat
     2101 gtctcgcaag gtggcgcttt cacgattggc aagaccatct gtgatgaaca cgcgatcgag
     2161 atcccagatc gtcaagccat agttctcgat atccagttcc gggtgataaa gaatcgggag
     2221 ggcgtggagc ggatcggtgt cggcgatcaa atgaccacgc acacgatacg cgttgatcaa
     2281 ctcgatcacg cgtgcttcct tgagagtttg ttcgcgcacc cgatcttcgg tcaggaaaaa
     2341 cggattgcga tcgacgttcc aacgcatcgg agcgtgcgga atcccgaggt tcagaaaaat
     2401 gttcttgtaa aaatcgtgct tgccgatgag gaactcgtgc acgcgcgcta gaaacgcccc
     2461 cgattccgcg ccctgaatga tgcgatgatc gtaagtgctg ctgattgaaa tcgctttgct
//
LOCUS       DASSFQ010000067        16623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1361377, whole genome shotgun sequence.
ACCESSION   DASSFQ010000067 DASSFQ010000000
VERSION     DASSFQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361377"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1143
                     /locus_tag="VFH96_02060"
     CDS             145..1143
                     /locus_tag="VFH96_02060"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET6972776.1"
                     /translation="MISAKAGGINAITGQADVHPKGESEWQQLSITDDLDSGDRVRTA
                     NDGRVEILLNPGSYLRVGGDSEVELLNNTLANLEVRLLRGTAIVEATGADGLELNINI
                     STPHAKLAIVRQGLYRLNVIPNDATELIVRKGRVILNDDSHTKVKGGNKVVFSATNVS
                     VAKLTKEEKKVQEDVENWSKQRAETLAKANRRINNQMMTSAFATFRDWDPFSRSSGLW
                     FYNRIAGCYTFLPFYYGWGSPYGNSYSTSFYSPFGFPRAYWPRQPANTYPNGSNGTPS
                     GGTPSGGYTHPTRPVTTPTTNPSGSLGDPAGRRERMIDPDTGARMPRKNLEPSRPN"
     gene            complement(1245..1619)
                     /locus_tag="VFH96_02065"
     CDS             complement(1245..1619)
                     /locus_tag="VFH96_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6972777.1"
                     /translation="MFVTVDERDARPLYQQLVDEIKTLIARGELEEGTLLPPVRQVAS
                     DLGVNLNTVAFAYRRLQKEGLIRVKHGSGAVVISQKLKDKPDDRLRAQLRTALTQLIL
                     AGLKQTEIKALINEELEELQKN"
     gene            complement(1665..2369)
                     /locus_tag="VFH96_02070"
     CDS             complement(1665..2369)
                     /locus_tag="VFH96_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972778.1"
                     /translation="MMNWSRVCFGLALALLCVSSTTILAQNGDSSKATDEKLVYADFQ
                     KVENGRLVSSRGGRTQLNRWAQNMGAAPKMMGLENADPPGPAPARVTDQDTAAAFQYE
                     MRMPNEWAGVNMEVFGQPEKDGKPVADDISGYKYITVRIFGKGPQSVRVELVSRGLGL
                     NLESGYPQMTFRISPGFNTYKFKLDGFRQPEWATHVDFKRDILMKLTSVTIGVFCDKC
                     IVESGTVVVDNLAFEK"
     gene            complement(2497..3579)
                     /locus_tag="VFH96_02075"
     CDS             complement(2497..3579)
                     /locus_tag="VFH96_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET6972779.1"
                     /translation="MSAYDVVVVGGGIGGLTVAALLSARGVNTCLLERQSQVGGCIAR
                     VEFSGYDFEPGMGLYTSFGAGEVYDQLFSQLPVATPEVSLITSPYILRRADGSDISLE
                     TDDLYSIVGGPATLAERLAESIKQSGGNLRLNSPVLRLAYDEAGRAIGIDLLSGERVF
                     AKKAIVSNLTIWDTYGKLIGLNRTPAEIKKRLSSVHGAGVYVLYAGIEEAAVARLPAE
                     LMRVEDDEGEFTLAISSSGVAPPGKRPVTFTTRTEIDSWFAYQSSEEDYEQWDQDALE
                     HFWNRLHAALPELGSDIEVIETANPRTYYDSTRRKLGMVLGVEELVSVGYQTSVSNVF
                     MVGDTAISEAKVDAVVQSAFSLQSGI"
     gene            complement(3589..4491)
                     /locus_tag="VFH96_02080"
     CDS             complement(3589..4491)
                     /locus_tag="VFH96_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET6972780.1"
                     /translation="MTANNQAPKLIEVFTEESSQQLTPLPEEHAEPPEPRPSLAVQPT
                     KEETWVSAWRDVGAGMGVWIASVVLLLVVPLIYSLPYLVYRIAKYGPPTPESLATDKQ
                     LIFYSVIGIIPTHMLTFVIVWLLITYGGRRPFWKNIEFEWPSNASPVVVTLVSVLVAT
                     LLFLLALGVTSLYGEHKTDLDLLIESSMYTRVATAFVAVATAPLVEEIVYRGLLYRTL
                     EKAAGMGVAIALVSLLFAGVHVFQYRNNLAVIIVITLLSITLTVSRAVTGKLLPAFII
                     HLVFNGIQSVLIVLGGFIDKDIFK"
     gene            complement(4504..5070)
                     /locus_tag="VFH96_02085"
     CDS             complement(4504..5070)
                     /locus_tag="VFH96_02085"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6972781.1"
                     /translation="MTKELPKILSSQKVFDGRVFKVTVDTVVEGELTYQRDVVHHPGS
                     AVIVPVHDDGTVVLVRQYRHPAVRYLLEVPAGTLSDGERPEAGAARELEEELGLVAER
                     LEKLSEFFVSPGFLEEKMWVYLATGLTEGKPQPDEDEVLDVVRLPIGDALEMITSGEI
                     QDAKSIIGLILAAPRVGAPLLDIEYPAV"
     gene            complement(5063..6295)
                     /locus_tag="VFH96_02090"
     CDS             complement(5063..6295)
                     /locus_tag="VFH96_02090"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET6972782.1"
                     /translation="MNFEAAVEYLLSLGHETLAIKLGLRNTELLLNALDNPECAFQSV
                     QIAGTNGKGSTAAMLDSICRAAGIKTGLYTSPHLVSITERIKFSGTEITREEFAACAT
                     TVREIAEQLEARPTFFEQVTAIALLAFRKAHIELAILETGLGGRLDSTTAANAHIVAI
                     TQIAMDHQEYLGNTIASIAAEKAAIIRPGVRAVIGRQHYPEALEVLLERCKETGVTPI
                     LVDDDSYSNAARITLSLRGRHQIENAAVAIHLAELLDIPQAAIVRGLETAQHPGRLEL
                     IPHQPPFLIDGAHNPAGAESLRAYLDEFAQRPLTLVFGAMRDKQLEQIGEILFPVTDV
                     LVLTTVDNPRSATLEMLQPIAMRYAQGNVLQSGSSTIALQTALANTPANGMICVAGSL
                     YLIGELRPQILSLGKQND"
     gene            complement(6360..7268)
                     /gene="accD"
                     /locus_tag="VFH96_02095"
     CDS             complement(6360..7268)
                     /gene="accD"
                     /locus_tag="VFH96_02095"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET6972783.1"
                     /translation="MAWFSKKSKRIEAVPPEERVVNTQNVFVKCDGCEEHLFTGELEA
                     ALQVCKHCNYHFRLGARERLRMLFDDGKFEELDGEVISIDPLEFVDSKPYSERIELAK
                     ESSGLPEAVINGRGIVGDHPVLAAAMDMSFIGGSMGSAVGEKVTRLIERAMDEHSAVI
                     IFSASGGARMQEGTLSLMQMAKISAALAALDEARLPFISVLTDPTTGGVTASFAMLGD
                     VIIAEPKALIGFAGPRVIEQTIRQKLPKDFQRSEFLLDHGMIDAIVDRRELRDYLVKL
                     LSFMMNPEIHGESDIERARRAAKLKE"
     gene            complement(7320..7898)
                     /gene="cobO"
                     /locus_tag="VFH96_02100"
     CDS             complement(7320..7898)
                     /gene="cobO"
                     /locus_tag="VFH96_02100"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET6972784.1"
                     /translation="MSGIDKKYKWRRADYQEKTRGLLMVHTGDGKGKTTCALGLMMRA
                     AGRGLKCCMIQFMKARNDRYGEHVSAEKLGIEVHTMGDGFTWDTKNPEQDIKTARETW
                     ALCVEKLRSGDYDMLVFDELVYVLSYNMLPVAEVMEEIRAIRASQPALHIVVTGRDAP
                     PELIEAADLVTEMKEIKHPFTAGIRAQQGIEF"
     gene            complement(7985..8992)
                     /locus_tag="VFH96_02105"
     CDS             complement(7985..8992)
                     /locus_tag="VFH96_02105"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET6972785.1"
                     /translation="MLSQVVELIEAKRRFAITSHIRPDGDSLGSSLGLYWLLRALDKD
                     VEVIMRDPVPHAYQQLPGAGDVRVTPAVDPSYHAVFVIECSDITRPGLADLEKQFVVN
                     IDHHSTTALFGNINWIDSTASAVGEMIYNLCKATGVRVTKEIAECVYTALITDTGSFH
                     YSNTTERTFKVASELVRTGVKPAKTAEAVFASYPWSRIQLMGAVLSTARRDESGRVAC
                     MRQSLEMQQQAHASDEDADGFVNYPLTVGEVEAVAMLKESEPGVYRTSLRSKGDVNVA
                     KVAEKFGGGGHRNAAGCTLKGSWEEAETTIVALLQDAVKRANGARDLTEDALKETESV
                     I"
     gene            complement(9065..9430)
                     /gene="rbfA"
                     /locus_tag="VFH96_02110"
     CDS             complement(9065..9430)
                     /gene="rbfA"
                     /locus_tag="VFH96_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET6972786.1"
                     /translation="MRRAERTADTLREEIAQIVGYELEDPRLTMVTVTDVRLSADKRA
                     ARVYVTIAGDENEHKAALAALKHAAPFVRKQLGLSLNLPRTPEIHFVRDRVEEEGERV
                     DQLLQEIEREWESKGKEES"
     gene            complement(9446..12259)
                     /gene="infB"
                     /locus_tag="VFH96_02115"
     CDS             complement(9446..12259)
                     /gene="infB"
                     /locus_tag="VFH96_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET6972787.1"
                     /translation="MTVSSKVRIYDLAKELKLDTKRLIEEVRREGVDVSVPSNSVSKE
                     LAEKIRNKYFPKKETAVKRAVKVVKRAVLPEAPAIDAELEIHESEPPPPPAPVAAEPV
                     EAPIAATPVAPATSPGPGPQPAKPPITSTRLVKKLAPAARAEAPVIEETPPPPAPVEV
                     APVAEEPVIEEPVILPEAVAPTNGIEVKVTPPPAPAPPRTPPPPSRQVRVLRPTAAAL
                     NAGIRPGERAPAPPPPPPPPTREREREPQRPTDRNRLRERPSLPRSERLGTPGETATP
                     QTTYIPPPDSGRRRSRRSSPRGGRKIEGKAGRFDRGGDFIPPPKALSLEDRIAGRLEM
                     PGSGELKEVRLIEGSTVKEFAEKLGIKPKDIVTLLLQRGVFATINQPLMDDVAADLGK
                     RFGYDVSFVPFEEMVAEEEFEELIATDADDVELPRAPVITVMGHVDHGKTSLLDAIRT
                     TDVAGGEAGGITQHIGAYSVQVPNPDNPGETRRVVFLDTPGHEAFTMMRARGAKATDI
                     VVLVVAADDGVMPQTIEAIEHSRAAGVPIIVAINKVDKPDANPDRVKQELAQQGLSPL
                     EWGGETEMVAVSAKKRQNLDTLLETILLTSDILNLRASSTRLASGVVLEAKLDRGRGA
                     VATVLVQQGTLKIHEPFIVGQISGKVRAMFNDRGEPITEAGPATPVEVLGLQGVPQAG
                     ESFQVVSDITKAQSISQHRQMLSRQAALLQTTKRGIEALGESEVKELLVIIKADVQGS
                     VEVLKSTLQKLSTDRVKVKVIRSGVGAITESDVLLGSATQAGSSSSAVVIIGFNVRPE
                     ARAADVAKQEAVDIRLHSIIYKVEEEIRAAMIGMLEAIEKEKILGKAAVREVFRVPRA
                     GVVAGCMVIDGTIRRNAKARLIRDGVVSWEGNIASLRRFKDDVSEVREGFECGISLEN
                     FNDLKVGDQIEAYIVERVAATEL"
     gene            complement(12428..14155)
                     /gene="nusA"
                     /locus_tag="VFH96_02120"
     CDS             complement(12428..14155)
                     /gene="nusA"
                     /locus_tag="VFH96_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET6972788.1"
                     /translation="MSSSIAQNIEALCQEQGIDRDLVIEAMKEAVRAAAKKQFRSGED
                     IQVDWNAETGIELSASKVVVDEVENPATELSIDEARELAGDEVELGDALLLPLPMEEL
                     GRIAAQTAKQILFQKVRDAVRSNVYEQYIDKIGDLVNGYVKRFERGNIIVDLGNLEAI
                     LPRSQQSRGEQWNQGERIRVVINNVSKESKGPQVEVSRTSPELLLRLFEMEVPEIYDG
                     TVVIKSAVREPGERAKIAVMSNERDVDPVGACVGMKGSRVQAIIRELRGEKIDIIEWS
                     DEPSVFAANALSPAKVNQVRITDIENRQMEVIVNEDQLSLAIGKRGQNVRLATKLVGW
                     NIDIRSEEEIKREVTEQMGALIASGEAVPLSAIEGVTAAQAEALAEHGINDIDALAAT
                     TVDDLVEFLDLSLDEAEVILEASKAITALRDLSIGEDGEEIEEQHAVSAEFDESIDEE
                     VEPSLESTAEGYDEAVENGVPFSSEPEIPARDSADPVPVTEAEAIPADEIALQEAGRD
                     LRPDTITPSPDITSSGVEAFEHIDEVVQEMEDEEFASDATAEEAETSAPAVSEETASG
                     GSTDDDENH"
     gene            complement(14211..14696)
                     /gene="rimP"
                     /locus_tag="VFH96_02125"
     CDS             complement(14211..14696)
                     /gene="rimP"
                     /locus_tag="VFH96_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET6972789.1"
                     /translation="MGNESIAERVQELAERVAIDHGLELVHAEVAGPENKPIVRIFID
                     KPQGVTHEDCSAVSTHLGTILDVEDFIHAPYTLEVSSPGLERGLYKRADFERFAGNNA
                     KMKTRQPIDGQRNFRGRLLGVEGDAVLFEDRTSGQVRVPLDTITKANIEVDVSEELRR
                     H"
     gene            complement(14891..15340)
                     /locus_tag="VFH96_02130"
     CDS             complement(14891..15340)
                     /locus_tag="VFH96_02130"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET6972790.1"
                     /translation="MSLSKQIVADLTSAMKAQDANRTSTLRMVKAAMMNRQIEKGSEL
                     DDDDMQKLLRSLVKQRRDSIEQYEKAGRQELVDKEKAEIDVIETYLPQAVSQEEIEQV
                     VTAAIVETGATSMKDMGKVMKAAQAALAGKNADGRLVSEVVKARLNS"
     gene            complement(15384..16514)
                     /locus_tag="VFH96_02135"
     CDS             complement(15384..16514)
                     /locus_tag="VFH96_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6972791.1"
                     /translation="MRNQTATSNKIPLRRRLLYIGFIYVVFLLLLLSVELVTRLTMKR
                     VESLDLFVNTVQQKMQVASPQQSTIFEGDPLLLWRLKPNLDHVYWDFTSVSTNAQSFR
                     ADYPIGRKPAGTFRIVCLGDSVTFGYRVPPVWPEKPKEYDPEWQPYPMLLEKELRKAN
                     PNRSIEVFPMAVPGYTSHQGLAWLRRDIGYLRPDMVIASFGWNDASLSDAPDRELIDT
                     RWSSVTGRWLIDHSQAIAHAARWFRSRKDVKPVVHTPVSRVSQQDYVDNFNEIVRVAK
                     DHGAAVIVIGAPYRDSVTNPPEAQLMTQYRAWLKAEMQQSQTPYLEILELTEAAGSVN
                     QGFFGELIHPNHMGHRLIASELLEMMSERHLLGDLKVPVFVP"
BASE COUNT         3465 a         4941 c         4444 g         3773 t
ORIGIN      
        1 tagcgcccgc ccacaatctt tatcgcgaat tcggagggta tttcgatgag aaaagctttc
       61 agcctcctgg cgtcgttcac tctttcagtg gctttcatac tgtgctgctc gatcgtcaac
      121 tttgctcaaa atcgggaaaa attcgtgatt tccgccaagg cgggcgggat taatgccatc
      181 actggccagg ccgacgttca cccgaaaggt gagtctgagt ggcaacagct ctcgattacc
      241 gacgatttgg attccggcga tcgcgtgcgg actgcgaacg atggtcgggt cgagattctc
      301 ctgaatccgg gttcctacct gcgtgtgggc ggcgattctg aagttgagct actgaacaat
      361 acgctggcga atttggaggt tcgcctgctg cgcggcaccg cgatcgtgga agctaccggt
      421 gcggacgggc tcgagctcaa tatcaacatc agtactccgc atgcaaaact cgcgattgtg
      481 cgacaagggc tctatcgttt gaatgtgatt ccaaacgacg ccaccgagct gatcgtccgc
      541 aaaggtcggg tgatactcaa cgacgattcg cacacgaagg ttaagggtgg caacaaggtc
      601 gttttcagcg ctacaaacgt ctcggtcgca aaactgacga aggaagaaaa gaaggttcag
      661 gaagacgtcg aaaactggag taagcagcgc gcggaaacgc tcgcgaaagc gaatcgacgc
      721 atcaacaacc agatgatgac gtcggcgttt gcgacttttc gagactggga tccgttctcg
      781 cgctcttccg gattgtggtt ctacaacagg attgccggct gttacacgtt cctgccgttc
      841 tattacggat gggggtcgcc gtacggcaat tcttactcga cgtcatttta cagtccgttt
      901 ggtttcccaa gagcttactg gcctcggcaa ccggcaaata cttatccgaa cggctcgaat
      961 ggcacaccct caggcggaac accttcgggc ggctacacgc atccgacgcg tccagtcacg
     1021 acaccgacaa ccaatccatc aggctctctc ggagacccgg cgggccgccg cgaacgcatg
     1081 atcgatcccg acacgggcgc tcgcatgccc cgcaagaacc ttgagccatc gcgccctaat
     1141 taagaactag ccacaaaagg cacaaaaagc acaaaagaga aatggaatgg aatggggtca
     1201 acatcccaac accttctctt ttgtgctttt tgtgcctttt gtggctagtt cttctgtaat
     1261 tcttcgagct cttcgttgat aagggctttg atctcggttt gtttgaggcc ggcgaggatg
     1321 agttgggtca gggcggtgcg taattgagcg cgcaggcggt cgtcaggctt gtctttcagc
     1381 ttttgcgata tgacaaccgc ccctgagccg tgtttgacgc gaatcagccc ctctttttgc
     1441 aaacggcgat aggcgaaagc gactgtgttc agattcaccc caaggtccga cgccacctgc
     1501 cttactggcg gcaaaagcgt cccttcctcc agctcacccc gcgcaatcag cgtcttaatc
     1561 tcatctacca gttgctggta cagcggccgc gcgtctcgct catctaccgt tacgaacata
     1621 ttgtctgttg aatatataca ctagacaatc tgatctggga agtcttactt ttcgaacgcg
     1681 aggttatcca ccaccaccgt gcctgactcc acgatgcatt tgtcgcagaa cactccgata
     1741 gttacagagg ttagcttcat caatatatcg cgcttgaaat cgacgtgcgt ggcccactcc
     1801 ggctggcgaa atccgtccag cttgaacttg taggtgttga atcccggcga gatgcgaaac
     1861 gtcatttgcg gataaccgga ttcgagatta aggcccagac ctcgggatac tagctcaact
     1921 cgaactgact gtggcccttt gccgaagatg cgcacggtga tgtacttgta gccactaata
     1981 tcgtctgcga ccggcttgcc gtctttctcg ggctgaccaa agacttccat attcacccca
     2041 gcccactcgt ttggcatacg catctcgtac tgaaatgccg ccgcggtatc ctgatcggtg
     2101 actcgtgccg gagccgggcc tggagggtcg gcattttcga ggcccatcat tttcggcgca
     2161 gcacccatgt tttgggccca gcgatttagc tgagtgcggc caccacgcga actcaccagg
     2221 cgtccgttct cgaccttttg gaagtctgca taaaccaact tctcgtcggt ggccttcgag
     2281 ctgtcgccgt tttgggccag tatcgtagtg ctgctaacac acaacagagc aagcgctaat
     2341 ccaaagcaca cgcgcgacca attcatcatt gtcgtactcc tgaataggga tttggattac
     2401 cttgataagc cctagcatac gttctctgtc aagtttgatg tccgataagc tttagcttgt
     2461 cggaatgtcg cgacaaacta acgtttatcg gacaccctat atcccgcttt gcaacgagaa
     2521 cgctgattgc acgacggcat cgactttggc ctccgaaata gctgtgtctc ccaccataaa
     2581 cacattcgaa accgacgttt gatagccaac agaaaccagc tcctcgactc cgagcaccat
     2641 tccgagcttg cggcgagttg agtcgtaata agtgcgtggg ttcgcggttt cgattacttc
     2701 gatatcgctg ccgagctcgg gcagcgcagc atgcaatcgg ttccagaaat gctccagcgc
     2761 gtcctggtcc cattgctcgt aatcttcttc actagactgg tatgcaaacc aggaatcgat
     2821 ctctgtgcgc gtcgtgaagg tcaccgggcg tttcccaggc ggtgccactc cactcgacga
     2881 aatcgctaag gtgaactcac cctcatcgtc ttcgacacgc atcagttccg ccggtagtcg
     2941 cgccacggct gcttcctcaa tccctgcata taaaacgtag acacctgcgc cgtgaacgga
     3001 actcaagcgc ttcttgattt cagccggcgt gcgattcaga ccgatgagtt tgccgtacgt
     3061 atcccagatc gtgaggttgc tgacgatcgc ctttttcgca aaaactcttt ctccgctcag
     3121 cagatcgatt cctatcgcgc gtcctgcctc gtcgtacgca agccgcagca ccggggaatt
     3181 caaccgcagg ttaccgccac tctgcttgat cgattcagcc agacgctcag ccagggtcgc
     3241 tggtcctcca acaatcgagt aaagatcgtc agtttctaaa gagatatcac ttccatctgc
     3301 gcggcgcaga atgtaaggcg aggtgatgag agacacttca ggcgtcgcga ccggaagctg
     3361 agaaaagagt tgatcgtaaa cttcgccggc gccgaaggaa gtgtatagcc ccattcccgg
     3421 ctcgaagtcg taaccggaga actccacgcg cgcgatgcac ccgccgacct gcgactgtct
     3481 ctcgagcagg cacgtgttta ctccacgcgc agataacaac gccgcgacgg tcagaccacc
     3541 aatcccgcct cccactacga cgacgtcgta tgcgctcatg cccagtctct acttgaaaat
     3601 atctttgtcg atgaaaccgc ccagcacgat gaggactgat tgaatgccgt taaaaacgag
     3661 gtgaatgatg aaggcaggaa ggagtttacc cgtaacagcg cgcgacaccg tcagcgtaat
     3721 actcagcagc gtgatgacaa tgatcaccgc gagattgttg cggtactgaa aaacgtgaac
     3781 gcccgcgaac aatagcgaca cgagtgcgat ggcgacgccc atgcccgctg ctttttcgag
     3841 tgtgcgatag agcaggccac ggtaaacaat ctcttcaacg aggggcgcgg tagcgacggc
     3901 cacgaatgct gttgccacac gcgtgtacat agagctctcg atcagaagat cgaggtctgt
     3961 cttgtgttct ccgtaaagcg aagtgacccc gagagccagg agaaaaagca gcgtcgccac
     4021 gagtacgctc acgagcgtca caaccaccgg actggcattg ctgggccact caaactcgat
     4081 attcttccag aacgggcgac ggcctccata cgtgatcaga agccacacaa tcacaaacgt
     4141 gagcatgtgc gtgggaataa tgccgatgac agagtaaaag atgagctgct tgtcggtagc
     4201 gagcgattcc ggtgtcggcg gaccgtattt ggcgatgcga taaacgagat atggaaggga
     4261 gtagatcagc ggaacgacca ggagcaacac gacacttgcg atccacacac ccatgccggc
     4321 cccaacgtcg cgccacgccg agacccaggt ctcctccttc gtaggctgaa ctgctaagct
     4381 cggccgcggc tccggtggct cagcgtgttc ttcaggaagc ggtgttagtt gctgcgatga
     4441 ctcttcggtg aacacttcga tgagtttagg tgcttgattg tttgcggtca aaccttagac
     4501 gtctcaaacc gctgggtatt cgatgtccaa caatggtgct ccgacgcgtg gcgccgcaag
     4561 gatcaacccg atgatggact tcgcatcctg aatctcgcca ctcgtgatca tctcgagcgc
     4621 atcgccgatc ggcaaacgaa caacatccag cacttcgtct tcgtcaggtt gtggtttgcc
     4681 ttccgtcaat ccggtggcga gatagaccca catcttctct tcaagaaaac ccggtgacac
     4741 aaagaactcc gaaagtttct cgagccgttc ggccacgagt ccgagttcct cttcgagctc
     4801 gcgcgcggcg ccggcttccg gacgctcgcc atccgagagc gtgcctgccg gaacttcgag
     4861 caggtaacga accgcagggt ggcgatactg tctcaccagc acgactgtgc cgtcgtcgtg
     4921 gaccggcacg ataacggcgc tccccggatg atggaccacg tcgcgttgat aggtaagctc
     4981 accttcaacg acggtatcga ccgtaacctt gaacacgcgg ccgtcgaata ctttttgact
     5041 gctcaggatc tttggtagct ctttagtcat tttgttttcc taaactcagg atctgcgggc
     5101 gcagctcgcc gatgagataa agcgagccgg ccacacaaat cattccgttc gcgggcgtgt
     5161 tggcaagcgc tgtttgcaac gctatcgtac tgctcccact ctgcaaaaca ttgccctgag
     5221 cataacgcat cgcgatgggt tggagcattt ccagcgtagc actgcgcggg ttatcgaccg
     5281 ttgttagaac gagaacgtca gtgaccggaa acagtatttc accgatctgc tcgagttgct
     5341 tatcacgcat tgctccaaaa accaacgtca gcggtcgttg ggcgaattcg tcgaggtacg
     5401 cgcgcaatga ttctgcgccc gcaggattgt gtgcgccgtc gattaaaaac ggcggttggt
     5461 gtgggatcaa ttccagtctg cccgggtgct gtgcggtttc gagtccgcgc acgattgcag
     5521 cttgcggaat atcaagcaac tcggcgagat gaatcgccac cgcggcgttt tcgatctggt
     5581 gacggccgcg cagcgagaga gtaattcgcg cggcgttcga gtaggaatca tcgtcgacga
     5641 gaatcggtgt cacaccggtt tctttgcaac gctcgagcaa aacctccaat gcttcggggt
     5701 agtgttgtct cccgatcacc gcacgcacac ccggacgaat gatcgcggct ttttcagcgg
     5761 cgatactcgc aatcgtgttg ccgagatact cctgatggtc cattgcgatc tgtgtgatgg
     5821 cgactatgtg tgcgtttgcg gcggtagtgg agtcgaggcg gccgcccagt cctgtttcga
     5881 gaatggcgag ctcgatatgc gccttacgaa aagcgagcag ggcgatcgcg gttacttgtt
     5941 cgaaaaaggt tggccgagct tcgagttgtt ctgcaatctc gcggacggtt gtcgcgcagg
     6001 ctgcaaactc ttcgcgcgtg atctcggtgc cggaaaactt gatgcgctca gtgatcgaga
     6061 cgaggtgagg tgacgtgtat agaccggtct tgattcctgc cgcccgacag attgaatcaa
     6121 gcatcgcggc agtcgagcct ttaccgttcg tgccggcgat ctggactgat tgaaatgcgc
     6181 attcgggatt gtcgagagcg ttgagaagaa gttcggtatt gcggaggccg agcttgatag
     6241 cgagagtctc atgcccgaga ctcaaaagat actcaacagc cgcctcaaaa ttcataagaa
     6301 tagttagcca cagattacac ggattaaatc cggatctaat ccgtgtaatc tgtggctaac
     6361 tattctttca gttttgcggc gcggcgggcg cgttcgatgt ctgactcgcc gtgtatctcg
     6421 gggttcatca tgaagctgag cagcttgacg aggtagtcgc gcagctcgcg gcgatcgacg
     6481 atggcatcga tcatcccgtg atcgagtagg aactcggagc gctgaaaatc cttcggcagc
     6541 ttttgtcgaa tcgtctgctc gatcactcgc ggaccggcaa atccaatgag cgccttcggc
     6601 tcggcgatga tcacgtcacc aagcatcgca aaactcgccg tcacgccacc cgtcgtcgga
     6661 tcggtcagca ctgaaataaa cggcaggcgc gcttcatcaa gcgccgcgag cgctgcggag
     6721 atcttcgcca tctgcatgag cgagagcgtg ccctcctgca tgcgggcgcc gcccgacgcg
     6781 gagaagatga tcaccgcgct gtgctcatcc atcgcacgct cgatcagtcg cgtgactttc
     6841 tcgccgaccg cagaacccat cgagccaccg atgaaactca tgtccatcgc agcggcaagc
     6901 accggatgat cgccaactat gccacgcccg ttgatgaccg cttccggcaa gcctgacgac
     6961 tctttggcca attcgattct ttccgagtaa ggcttgctgt cgacaaactc gagcggatcg
     7021 atcgagatca cctcgccatc gagctcttca aacttaccgt cgtcgaaaag cattcgcagc
     7081 cgttcgcgcg cgccgagacg gaagtgataa ttgcagtgtt tgcaaacctg gagcgcggct
     7141 tcgagctcgc ctgtgaacag gtgctcttca cacccgtcgc atttaacgaa gacattttga
     7201 gtgtttacga cgcgttcttc gggcgggact gcttcgattc gtttgctctt cttactgaac
     7261 caggccatag gaatgagggg aagggtagca caaaccgaaa atctgtggta tccgcgcggc
     7321 taaaactcaa tcccctgctg cgcgcgaatg ccggccgtaa atggatgctt aatctccttc
     7381 atttccgtca cgagatcggc ggcttcgatc aactccggcg gtgcatcgcg gcccgtgacg
     7441 acgatgtgca gcgccggttg tgacgcgcgt atcgcgcgaa tttcctccat cacctcggcg
     7501 accggcaaca tgttgtagct cagcacgtag acgagctcat cgaaaaccag catatcgtaa
     7561 tcacccgagc gcaacttctc tacgcagagc gcccacgtct cgcgtgcggt cttgatgtcc
     7621 tgctcgggat tcttcgtgtc ccacgtgaag ccatcgccca tcgtgtggac ttcgattccg
     7681 agtttctcgg cggagacatg ctcaccataa cggtcgttgc gcgccttcat gaactgaatc
     7741 atgcagcact tcaaaccgcg tccggcggcg cgcatcatca aaccaagcgc acacgtcgtc
     7801 ttgcctttac cgtcaccggt atggaccatc agcagtccgc gcgtcttctc ctggtagtcc
     7861 gcgcgacgcc acttgtattt cttatctatt cccgacataa gccacaaaaa gcatagccgc
     7921 aaaaaggcac aaaaggcaca aaagttaaga aaaacatttt gtgctttttg tgccttttcg
     7981 tggcttaaat aactgactca gtttctttca acgcgtcttc ggtgaggtcg cgggcgccgt
     8041 tcgcacgctt cactgcgtcc tgtaggaggg caacgatcgt cgtctcggct tcctcccagc
     8101 ttcccttgag cgtgcagccg gctgcgtttc gatgcccgcc gccgccaaac ttctcagcca
     8161 ctttcgcgac attgacatcg cctttagatc tcaaactcgt acgataaact cccggctcac
     8221 tctctttgag catcgccaca gcttcgactt cgccaacagt caacggatag ttaacaaacc
     8281 cgtctgcgtc ttcgtcagaa gcatgcgcct gttgttgcat ctcgagtgac tgccgcatgc
     8341 acgccacgcg cccgctttca tcgcggcgcg ccgtcgatag caccgcaccc atgagctgaa
     8401 tcctgctcca cgggtagctt gcaaacactg cctcggcggt cttcgcaggt ttgacgccgg
     8461 tgcgcacgag ctcggaagcg actttgaacg tgcgctcggt cgtgttcgag taatgaaacg
     8521 atccggtatc ggtgatcagc gccgtgtaga cgcattcagc aatctctttc gtgacgcgca
     8581 cgccggtcgc tttgcagagg ttgtagatca tctcaccgac tgccgaagca gttgaatcga
     8641 tccagttgat gttgccgaag agcgcggtgg tcgaatgatg atcgatgttg acgacgaact
     8701 gtttctcgag atcagcgagg ccgggccgcg tgatatcgga acactcgatc acaaacactg
     8761 cgtggtaaga aggatcgact gcgggagtga cgcgtacatc gccagctcct ggcagttgct
     8821 ggtaagcgtg tggtactgga tcgcgcatga tcacttcgac atccttatcg agcgcgcgca
     8881 acaaccagta aagaccgagt gacgatccca gactgtcacc gtcagggcga atgtgtgagg
     8941 tgatcgcgaa acgacgtttt gcctcgatca gctcgactac ttgactaagc ataattgcga
     9001 aattcaaaaa gagattctaa taggccctta agacctatag gacctattac gtcctattaa
     9061 ctcttcaact ctcttccttt cctttacttt cccattcccg ctcgatctcc tgtagcagtt
     9121 gatcgacgcg ttcgccttct tcttcaacgc gatcgcgcac gaaatgaatc tcaggtgtgc
     9181 gcggaagatt caacgagagc cccagttgtt tgcgcacgaa cggcgcggcg tgcttcaacg
     9241 ctgcgagggc cgctttgtgc tcgttctcgt caccggctat ggttacgtaa acacgtgcgg
     9301 cgcgcttgtc agccgaaagc cgaacgtccg taaccgtgac catcgtcaac cgcggatctt
     9361 ccaattcata gccgacaatc tgagcgatct cttctcttag agtgtcggcc gttctctctg
     9421 ctcgacgcat aaaccttctg cgcctttaca actcagtggc cgcgactcgc tcgacgatgt
     9481 aggcctcaat ctggtcgccg actttcaggt cgttgaagtt ttccagactg ataccacact
     9541 cgaagccctc gcggacctcg gacacgtcat ccttgaaccg ccgcaacgaa gcgatgttgc
     9601 cttcccagct cactacgcca tcgcgaatca agcgcgcctt cgcattgcga cgtatcgttc
     9661 catcgatgac catgcagccg gcgacaactc ccgctctcgg tacgcggaaa acttcgcgga
     9721 ctgccgcctt gccgagaatt ttctccttct cgatcgcttc gagcatgccg atcatggccg
     9781 cgcgaatctc ttcctcgacc ttgtagatga tcgagtggag tcgaatgtcg actgcttcct
     9841 gtttcgcgac gtctgccgca cgtgcttcgg gtcgcacgtt gaaaccgatg atcacgactg
     9901 ccgacgacga cgagccggcc tgcgtcgcag aaccaagcag tacgtctgac tcggtaatcg
     9961 cgcccacgcc cgatcgaatc actttcacct tcacccgatc ggtcgagagt ttttgcaggg
    10021 tcgacttcaa cacttcgact gaaccctgca cgtcggcctt gatgatgacc agcagctcct
    10081 tgacttcgct ctccccgagc gcttcgatac cgcgcttcgt cgtttgcagc aatgccgcct
    10141 ggcgactgag catctggcga tgctgtgata tcgactgcgc tttcgtgatg tcggaaacga
    10201 cctggaagct ctcgccggcc tgcggcaccc cttgcaaacc aagcacttcg accggagttg
    10261 ccggtccggc ttccgtgatc ggctcgcccc gatcgttgaa catcgcacga accttgccgg
    10321 atatctgtcc gacaatgaaa ggctcgtgaa tcttgagcgt tccctgttgg accagaacgg
    10381 tcgctactgc accgcggccc cgatcgagtt tcgcttccag cacgacgccc gaagcgagac
    10441 gcgtcgagct cgcgcgcagg ttcagaatgt ctgatgtcaa cagaatcgtt tcgagcaggg
    10501 tgtcgaggtt ctgccgcttc ttcgcggaga ccgcgaccat ttctgtctcg ccgccccact
    10561 ccagtggcga tagaccctgc tgggccagtt cctgtttgac gcggtcagga ttcgcgtcgg
    10621 gtttgtcgac cttattgatg gcgacgatga tcggaacacc tgctgcacgc gagtgctcga
    10681 tagcttcaat cgtctgcggc atgacgccgt cgtccgcagc gaccaccagc acgacgatgt
    10741 ccgtcgcctt cgccccgcgc gcgcgcatca tcgtgaacgc ctcgtggccc ggcgtatcga
    10801 gaaacacgac gcgacgagtc tctcccggat tatccggatt cgggacctgc acgctgtacg
    10861 cgccgatatg ctgcgtgatg ccgcctgctt caccgccggc aacgtcagtt gtgcggatcg
    10921 cgtcaagcag cgacgtctta ccgtggtcga cgtggcccat gacagtaatg acaggagcac
    10981 gtggcagctc aacgtcgtca gcgtcagtgg cgatcagctc ttcaaactct tcttccgcca
    11041 ccatctcttc aaacggcacg aacgagacgt cgtaaccgaa tcgtttgccg agatcggccg
    11101 cgacgtcgtc catcaacggt tgattgatgg tcgcgaagac gccacgctgc aacaacaacg
    11161 tcacgatgtc tttcggtttg attccgagtt tctcggcgaa ctctttgacg gtcgaaccct
    11221 cgatgaggcg cacttctttc aactcgcctg agccgggcat ctcgagtcga ccggcaatac
    11281 ggtcttcgag tgacaaagcc ttcggcggcg gaatgaaatc accaccccgg tcaaacctcc
    11341 cggccttgcc ttcgatcttg cgaccgccgc gcgggctgct gcgtctcgaa cgacgacgac
    11401 cagaatccgg tggtggaatg taagtagttt gcggcgtggc cgtctcgccg ggtgttccga
    11461 gtcgttcgga gcgcggcaat gacggtcgct ctcgcaggcg attgcgatcc gtggggcgtt
    11521 gtggttctcg ttcccgctct cgagtcggag gcggcggtgg cggcggtggc gcgggcgcgc
    11581 gctcgccggg acgtatgccg gcgttgagcg cggctgcagt cggtctcaaa actctgactt
    11641 gccgcgaagg cggcggcgga gttcgcggtg gtgcaggcgc cggtggcgga gtgactttca
    11701 cttcaatgcc attcgtcggc gcaaccgctt ccggcaagat caccggctct tcgataacag
    11761 gctcttccgc caccggcgct acttcaaccg gcgctggcgg tggaggcgtt tcctcgatca
    11821 ctggcgcttc cgcgcgtgca gccggagcca gtttcttcac cagcctcgtg ctcgtgatcg
    11881 gcggcttggc gggttgcggc ccgggaccag gcgatgtcgc cggtgccacg ggtgtcgctg
    11941 caatcggagc ttcgacgggc tcggcggcta caggagccgg cggcggcggt ggctcagact
    12001 cgtggatctc gagctcagcg tcgatcgcgg gcgcttccgg cagcactgcg cgcttgacca
    12061 ctttgactgc gcgcttaacc gccgtttcct tcttcggaaa gtacttgttc cgaatcttct
    12121 cggcgagttc tttcgagacc gagttggaag gtacgctcac gtccacacct tcacgacgca
    12181 cctcttcgat gagtcgcttg gtatcgagct ttaattcttt ggcgagatcg taaatacgaa
    12241 ctttagagga aactgtcatg cactccttct cgacgcttct tcctgcgtcc tgacgaccta
    12301 agtgattggg caccgcatgc cccgtcactc agcgtcgctc agaagatcgg cgcctgacag
    12361 caggggcaat gcgggcaaca caaacggccc gaagagaccg catctgtgtt gttactatat
    12421 gactgcatca gtggttctca tcgtcgtctg tgctcccgcc tgatgcagtc tcttccgaaa
    12481 ctgcgggagc cgatgtctct gcttcttcag ccgtagcgtc tgaagcgaac tcttcgtctt
    12541 ccatctcctg tacaacttcg tcgatgtgtt cgaatgcctc gacacctgag gaagttatgt
    12601 caggagacgg cgtaatcgtg tcaggccgca aatcgcgacc tgcttcctgc aaagcaatct
    12661 cgtcggccgg gatcgcctcg gcttcagtca ccggtaccgg atcggccgaa tcgcgcgccg
    12721 gaatttccgg ttccgaagag aagggaacac cgttctcgac tgcttcgtcg tagccttccg
    12781 ccgtcgattc cagagacggt tccacttctt catcaatcga ttcgtcgaac tctgcagaaa
    12841 cggcgtgttg ctcttcaatc tcttcgccgt cttcaccgat actaagatcg cgcagggcgg
    12901 ttatggcctt ggaagcctcc aggatcactt ccgcttcgtc caaactcaga tcgaggaact
    12961 cgacgaggtc gtcgacggta gtggccgcga gagcatcgat atcgttgatg ccgtgttcgg
    13021 ccagcgcctc agcctgcgca gcggtgacac cttcgatagc ggagagcggc actgcctcgc
    13081 ccgacgcgat gagcgcgccc atctgctcgg taacttcgcg cttgatctct tcttcgctgc
    13141 ggatgtcgat gttccaacca acgagtttcg tagccaggcg cacgttttga ccgcgcttgc
    13201 cgattgccag cgagagttga tcctcgttga cgatgacttc catctggcgg ttttcgatgt
    13261 cagtgattct gacctgattg actttcgcag gtgaaagcgc attcgcggca aaaactgaag
    13321 gctcgtcaga ccattcgata atgtcgatct tctcgccgcg caactcgcgg atgatcgcct
    13381 gcacgcgtga acccttcata ccgacgcacg cgccgaccgg atcgacgtca cgctcgttcg
    13441 acatcacggc gatcttggcg cgctcgccgg gctcgcggac agccgatttg atcacgacag
    13501 tgccgtcgta gatttccggc acttccattt caaacaatct tagtaagagc tcgggagacg
    13561 tgcgcgagac ctcgacctgc ggtcctttcg actctttcga aacgttgttt atgacgacgc
    13621 gaatacgttc gccctggttc cactgctcgc cgcgtgactg ctgcgaacgc ggcagaatcg
    13681 cttcgagatt accgaggtcg acgatgatgt tgccgcgctc gaatcgtttg acgtaaccgt
    13741 ttacgaggtc gccgatcttg tcgatgtact gctcgtagac gttgctgcga acagcgtcac
    13801 ggactttctg aaacaggatc tgcttggctg tctgggcagc gatgcgtccc agctcttcca
    13861 tcggtaacgg caataaaagc gcgtcaccca attcgacttc gtcaccggcg agctcgcgtg
    13921 cttcgtcgat agagagttcg gtggcggggt tttcgacttc gtcgacgacg acttttgagg
    13981 ccgagagctc gatgccggtt tcggcattcc agtcgacctg aatatcttca ccggatcgaa
    14041 actgtttctt ggcagccgca cgtacggctt ccttcattgc ttcgatcacg aggtcgcggt
    14101 cgatgccctg ttcctggcaa agcgcctcga tattttgtgc tatcgatgaa ctcatcttct
    14161 attttgcccg atcttttata ggacctggac gtcctatatg acctattatt tcaatgccgg
    14221 cgcagctcct cgctaacatc cacttctata ttcgccttcg taatcgtatc cagaggaaca
    14281 cgcacctgcc cgctggttcg atcctcaaaa agcacggcgt cgccctcgac tccgagcaaa
    14341 cgacctctaa aattgcgctg tccgtcaatc ggctgccgcg tcttcatctt cgcgttatta
    14401 ccggcgaagc gttcgaagtc ggctcgtttg taaaggccgc gttcgagtcc cggggacgaa
    14461 acttccaacg tgtagggcgc gtggatgaag tcttccacgt ccagaatcgt cccgaggtgc
    14521 gtgcttactg cggagcagtc ctcgtgggtg acaccttgcg gcttatcaat gaatatacga
    14581 acaatcggct tgttctcggg gccggctacc tcggcgtgca ccagctcaag gccatgatcg
    14641 attgcgactc gttcagctag ctcctgaacc ctttctgcaa tcgactcgtt tcccattgtc
    14701 acctttcaaa aggttctcgc tccaaaagaa aaagtgggcg cgaaacccac cggacgagcc
    14761 cccgtgcctt cgagcaagct gtgaattata cggaaagggc ggggttcaag caaccgcgta
    14821 gagccagcca caaaaggcac aaaaagcaca aaagcagaaa gtgatttttg tgcctttgtg
    14881 ctttttgtgg ctatgaattc agcctcgctt taacgacctc gctgacgagg cgtccgtcgg
    14941 cgtttttgcc tgccaaagcg gcctgtgctg ccttcattac cttgcccatg tccttcattg
    15001 aggtcgcgcc tgtttcgacg atggcggctg tcaccacctg ttcaatctct tcctgtgaga
    15061 cggcctgggg caggtaagtc tcgatgacgt cgatctcggc tttctcttta tcgacgagct
    15121 cctgccgacc ggctttctcg tactgctcga tcgagtcgcg gcgctgcttc acgagcgagc
    15181 gcagcaactt ctgcatgtcg tcatcatcaa gctctgaacc cttctcgatt tggcggttca
    15241 tcatcgccgc cttaaccatc cgcagcgtcg acgtacgatt cgcgtcctgc gccttcatgg
    15301 cgctcgtcag atccgcgaca atctgcttac taagactcat atcctcactc ctaaccgtgt
    15361 aatcagtgta aatccgtggc taactaaggc acaaaaacag gaaccttcaa atcgccgagc
    15421 aagtgacgtt cggacatcat ctcgagcagc tcggacgcaa tcaaccggtg gcccatgtgg
    15481 ttcgggtgga tcagctcacc aaagaagccc tgattgacac tgccggccgc ttccgtcaat
    15541 tcaagtatct caaggtacgg tgtctggctc tgctgcatct ccgctttcaa ccacgcgcga
    15601 tactgcgtca tcaactgcgc ttccggcgga ttcgtaacgc tgtcgcgata cggcgcgccg
    15661 atcacgatca ccgccgcacc atgatcttta gcaacgcgaa cgatctcgtt aaagttgtcg
    15721 acataatcct gctgcgacac ccgcgacacc ggcgtgtgca caaccggctt cacatctttc
    15781 cgcgatctaa accaacgcgc tgcatgcgca atggcctggc tgtgatcgat caaccaacgc
    15841 ccagttaccg acgaccagcg cgtgtcgatc aactcacgat ccggcgcatc actcaaactc
    15901 gcatcgttcc aaccaaaact cgcaatcacc atgtccggtc gcaggtaacc gatatcacgt
    15961 cgcaaccacg ccagaccctg atgactcgtg tatcccggca ctgccatcgg aaacacttca
    16021 atgctccggt tcggattcgc cttccgcaac tccttctcga gcagcatcgg atacggctgc
    16081 cactccggat cgtattcctt cggcttctcg ggccaaaccg gcggcacgcg ataaccaaac
    16141 gtcaccgaat cacctagaca aacgatccga aacgtccccg ccggctttct tccgatcgga
    16201 taatcagccc gaaaactctg cgcgtttgtc gaaacactag tgaagtccca gtaaacgtgg
    16261 tccaggtttg gcttcaagcg ccacagcagc aacggatcgc cttcgaagat cgtcgactgc
    16321 tgcgggctcg cgacctgcat cttctgctgc accgtgttta caaacagatc gagactctcg
    16381 acgcgcttca tcgtgaggcg cgtgacgagc tcgaccgaca gcaagagcag caggaaaacg
    16441 acgtagataa atccgatgta aagcagtcgt cgccggagcg ggatcttatt tgatgtggcc
    16501 gtttgatttc gcaaggtagt cgtgcaaaaa ccgacccgtg tacgactcgg gaatgttcat
    16561 gatctcttcg ggcgtgccaa tgccgaccac gtaaccgccc gcgtcgccgc cttccgcgcc
    16621 gag
//
LOCUS       DASSFQ010000068         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_329027, whole genome shotgun sequence.
ACCESSION   DASSFQ010000068 DASSFQ010000000
VERSION     DASSFQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329027"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1259
                     /locus_tag="VFH96_02140"
     CDS             78..1259
                     /locus_tag="VFH96_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET6972792.1"
                     /translation="MSTSFSLTNAVTSGDESAVSALLAGGADVNESTNGGQTALILAV
                     IFGHTNVVRVLMNAGADPQLRDNLGLNAIEWAQRRGLTEAHNILTNKPETATRPKRIL
                     IPVEEPTKPLANTTPTPRIDEAKNSVSDEDKSRRWLAGVRQRLDEQAMRRLNRNEPPV
                     EPRFIREEPPSPPPPPIEVKKEAPVVETVETPVEKPRITGAPRILTPPVEAEKKGKRK
                     RCPQCNAIYNGDLVSYCAHHIVALVDADEPIVTEKPKSNAALFWILLVITLTGSVVAG
                     SLITTYVYKSKQAATRSAAEQQKIVQKGTPEVGVELAGKAVSLPEAECPVKGPDPVSG
                     TVVVRIMVDKNGQVYWARGSGGDWLMRGAATEAAMKSTFASDKLRGREVEGTITYTFT
                     P"
     gene            1352..>4158
                     /locus_tag="VFH96_02145"
     CDS             1352..>4158
                     /locus_tag="VFH96_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6972793.1"
                     /translation="MADSQQTRILPDELRTTVPLLGTVLRDRYRLDSELGRGGMGTVY
                     RATDLELQREVAVKVLSTTAQNSDARERLVREARAAAALNHPHIVTIHDVGEADGFPF
                     LVMELVAGPRLSKARPTEISRIVEIATQICDALEHAHTNQIVHRDLKPDNVLLSGTTD
                     SSNVKLADLGLALPAYGARISRAGVIVGTAAYMAPEQALGQTVDGRADLYALGVLLYE
                     LTTGRLPFTGDDPLTIVSQHVHAPIVPPRVLCTEIPPALETIIVRLLSKDPAQRFRTA
                     TETRTALLDSLKSKGEAVPAESAPAVAILDALSRGRLIGRNAELAEARELWQRAREGR
                     GHAVLLSGEPGAGKTRLAREINIQAAVDGALVLHGGCYEYEAATPYLPFVEAFRRLVR
                     EETNDERLRELLGDAATQIAKLAPEIETRLGPFPERQELAAHEERLLFFDAVVNVFSN
                     IARRQSLLFYADDLHWADRGTLWLLSHLLRQLRNERVLILGSYRETELDRAHPLAKSL
                     VDWNRERLVTRIILRRFNETETGDQLGALLGEQVSGEFAIAVHRETEGNPFFVEEVLK
                     ALIERGSVRRESGRWRRCDMDELLIPQSVKEAIGNRLDRVSQNTNEVLRVSAILGKVF
                     TFEELSAAAEQNEDALLDALDEATGAQLIAAGSGDSFSFTHDKIREVLYEELNPIRRR
                     RLHRHVAEGLERNCELFPCAVEKLAHHYIQAGDHQRGLEYAKQAAAEAARVFAFEEAI
                     AAYGRARDCAEALGLTEEQLAQEEAIGKAFMLHGDTILAAEHFERALALATDQSTRVR
                     LQSQAAASLVTTGDPRGGEYLREALLVLDPVTNPLETANALSTEARFHHLAGRHKKAI
                     EILLRAVELVAPTAAAENVSGFAATVISTIYPYTAGGYQHFGLYEESDHWARRAIAFG
                     EQHNILFAQASGIEFLGENSI"
BASE COUNT          958 a         1207 c         1164 g          829 t
ORIGIN      
        1 ctttctagac cgcggtgaca agattattca ggatgcacag ggcaatcctg tagaataacg
       61 cacgaggttt tcgtgcggtg agtacttcct tctcgctaac aaacgccgtt acatccggcg
      121 acgaaagtgc tgtgtctgca ctgctggctg gtggtgcgga cgtaaatgaa agcacgaacg
      181 gcggtcaaac ggcgcttatc cttgcggtca tcttcggaca cacaaatgtt gtgcgagtgt
      241 tgatgaatgc gggcgctgac ccgcagttgc gcgacaatct cggtctgaac gcgatcgagt
      301 gggcacagcg ccgaggacta acggaagcgc acaatatcct caccaacaag ccggaaaccg
      361 ccactcgacc aaaacgaatt ctcattccag tcgaagagcc tacgaagccg ctcgctaaca
      421 caactcctac tccacgcatc gacgaagcta agaattctgt ttcagacgaa gacaagagtc
      481 gacgctggct ggccggagta aggcaaaggc tcgacgagca ggcgatgcgg cgactgaatc
      541 gaaatgagcc gccagtggaa ccgcgtttca ttcgcgagga accaccgtcg ccgccaccgc
      601 caccgatcga agtcaagaaa gaagcgccag ttgtcgaaac ggttgaaacg ccggtagaga
      661 aaccgaggat cactggcgca ccaaggatac tgacgccgcc ggtcgaggca gagaaaaaag
      721 gcaagcgcaa acgatgcccg cagtgcaatg cgatctacaa cggcgatctg gtttcatatt
      781 gcgcacacca catcgtggcg ctcgtcgatg cagacgaacc gatcgtcacg gagaaaccaa
      841 aatcgaatgc agcgctgttc tggatactgc tggtcattac tctcacggga tccgtcgtcg
      901 caggctcact gatcactacc tacgtctaca aatcaaaaca ggcggcgaca cgcagcgcgg
      961 ccgaacaaca gaagatcgtg caaaaaggca cgccggaagt cggcgttgaa ctggccggca
     1021 aagccgtctc actacccgaa gcagagtgtc ctgtgaaagg acccgatccg gtttccggca
     1081 cagtggtagt gcgaatcatg gtagacaaga acggacaggt ttactgggcg cgcggttcgg
     1141 gcggcgattg gctcatgcgc ggcgccgcca ccgaggcggc aatgaaatca accttcgcct
     1201 ccgacaagct tcgtggccgc gaagtcgaag gaacgatcac ctacacgttc actccataat
     1261 cagccacaaa aaggcacaag aggcgcaaaa catttattta aatcaatttg tgccttttgt
     1321 gcttttttgt ggctagacct atccttgtgg catggccgac tcacagcaga ctcgcatcct
     1381 tccagacgag ttgcgcacga ctgttcctct gctcggcaca gtcctgcgtg atcgctatcg
     1441 actcgatagt gaactcggtc gcggtggtat gggaacggtt tatcgcgcca ccgatctcga
     1501 gctgcaaaga gaagtcgccg tcaaagttct ctcaaccacg gcgcagaact ctgacgcacg
     1561 tgaacgcctg gtgcgtgagg ctcgggcagc ggctgcactc aatcatccgc acatcgtcac
     1621 cattcacgac gtcggcgaag ctgatggctt tccatttctg gtgatggaac tcgtcgccgg
     1681 gccgcgtcta tccaaagcgc gtcccactga aatctcgcgc atcgtcgaaa tcgcaactca
     1741 gatttgtgac gcactcgaac acgcgcacac caaccagatc gttcatcgcg atctgaaacc
     1801 cgacaacgtg ctgctgtcgg gaacaaccga ttcgagcaac gtgaagctcg cggatctcgg
     1861 actcgctttg cctgcttatg gcgcgcgcat ctcgcgcgcc ggcgtcattg tcggcactgc
     1921 cgcatacatg gcccctgaac aagcgttggg ccaaacagtc gatggtcgcg cagatctcta
     1981 cgcgctcggc gttctgctct acgagttgac gaccggtcgc ctgcccttca ccggcgatga
     2041 tccgctgacg atcgtttcgc agcacgtcca tgcgccgatc gttccgccac gtgtgctctg
     2101 tactgaaatt ccacccgcac tcgaaaccat catcgtgcgg ctgctttcaa aagatccggc
     2161 ccaacgattc cgcacggcaa ctgagacgcg cacggcatta ctcgattcgc tgaagagcaa
     2221 gggtgaagct gtaccggccg aaagcgcgcc ggcagtcgcg attctcgatg cgctttcacg
     2281 cggccgattg attggtcgta acgccgaact cgccgaagca cgcgagttgt ggcaacgcgc
     2341 tcgtgaagga cgcggacacg ccgtgcttct cagcggcgaa ccgggcgccg gcaagactcg
     2401 tctcgcgcgt gagatcaaca tccaggcggc ggttgatggc gcgctcgtac tgcacggcgg
     2461 ttgttacgag tacgaagcag ctacgcctta ccttccgttt gtcgaggcat ttcgtcgttt
     2521 ggtgcgggaa gaaaccaatg acgaaaggct gcgcgagcta ctcggcgatg cagccactca
     2581 gatcgcaaaa ctcgctcccg aaatcgaaac gcggctcggt ccgtttccgg aacgacagga
     2641 actcgccgcg cacgaagaac gtctgctgtt cttcgacgcg gtagtcaacg tgttttccaa
     2701 catcgcgcgg cggcagtcgt tgctcttcta cgccgacgac ttgcactggg ccgatcgcgg
     2761 cacgctctgg ctgttgagtc atctgctgcg tcaactacgc aatgaacggg tcctgattct
     2821 cggctcttat cgagaaacag aactcgaccg cgcacatccg ctcgccaaat ccctcgtcga
     2881 ctggaatcgc gagcgcttag ttacccgaat cattttgcgt cgcttcaacg aaactgaaac
     2941 aggcgatcaa ctcggcgcgt tgctgggcga acaagtgagt ggcgagtttg cgatcgctgt
     3001 tcatcgtgaa accgaaggta atccattctt tgtcgaggaa gtcttgaaag ctttgatcga
     3061 gcgcggttca gtgcgtcgtg agagtggacg ttggcggcgt tgcgacatgg acgagctgct
     3121 gatacctcag agtgtcaagg aagcaatcgg caatcgtctt gatcgcgtca gtcaaaacac
     3181 aaacgaagtg ttacgcgtga gcgcgatact cggcaaagtc ttcaccttcg aagaattgtc
     3241 agccgctgcg gagcaaaacg aagacgcatt gctcgacgct ctcgacgaag ctaccggcgc
     3301 acaactgatc gccgccggct ccggcgattc gttcagtttc acgcacgaca agattcgcga
     3361 ggttctttac gaagagctaa atccgatccg gcgtcgccga ctgcaccgtc acgtggccga
     3421 aggattggag cgcaactgtg aactgtttcc gtgtgcagtt gagaaactcg cgcatcatta
     3481 cattcaggcc ggcgatcacc aacgcggtct cgaatacgcc aagcaggctg ccgccgaagc
     3541 ggcacgcgtg tttgcattcg aggaagcaat cgccgcttac ggtcgcgcgc gtgattgcgc
     3601 cgaagctcta ggactaactg aagagcaact cgcccaggaa gaagcaatcg gcaaagcctt
     3661 catgctccac ggcgacacga tcctcgctgc cgaacatttc gaacgagcgc tcgcgctggc
     3721 cacagatcag tcaacacgtg tgcgtctgca atcacaggcg gcggcgtctc ttgtgaccac
     3781 cggtgatccg cgcggtggtg aatacttgcg cgaagcactc ctggttttag atccggtgac
     3841 taatccgctt gaaacagcga atgcgttatc gacggaagcg cgtttccatc acctcgccgg
     3901 ccgtcacaaa aaggcgattg agatcctgtt gcgcgccgtg gaactggttg ctccgactgc
     3961 ggcagctgaa aatgtcagcg gctttgctgc gactgtgatt tcgacgatct acccttacac
     4021 agcaggcggt tatcaacact tcgggcttta cgaggagtca gatcattggg cgcgacgtgc
     4081 aattgcgttt ggtgagcagc ataacatttt gttcgcgcag gcgtccggca tcgagtttct
     4141 cggagaaaac agtattca
//
LOCUS       DASSFQ010000069        12339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_625409, whole genome shotgun sequence.
ACCESSION   DASSFQ010000069 DASSFQ010000000
VERSION     DASSFQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625409"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..750)
                     /gene="rplQ"
                     /locus_tag="VFH96_02150"
     CDS             complement(163..750)
                     /gene="rplQ"
                     /locus_tag="VFH96_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET6972794.1"
                     /translation="MRHLKAHRKLGRTSEHRNSMLRNLATSLINSREERIVTTLPKAK
                     ELRPFVERAITLSRRARSLADNGSDLQALHLRRQAAGFFHAGNTTVKEVTGKRGQLRP
                     ERTAGVAALRRLFTELGDRYKDRPGGYTRIFKLGHRDGDAAELAIIELVDNPREVEAA
                     EAAKKRKAATSKKRKGRDKKAAAEDEATGTESGEE"
     gene            complement(760..1815)
                     /locus_tag="VFH96_02155"
     CDS             complement(760..1815)
                     /locus_tag="VFH96_02155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET6972795.1"
                     /translation="MAMDQNNLWTGFQMPRRLAADAETLTERYGRFSAQPFERGFGTT
                     VGNSLRRALLSSIEGAAITAVKIEGVEHEFSSIKGVVEDATDVILNLKQVPFKLHTSE
                     PKTLRINKEEAGEVTAADIETDADVEILDPSVHIATVSDGGSLKVEMRLKRGRGYVSA
                     DRNFDEDLSLGYIPIDSVHTPVKKVNYNVEAARLGQNTEFDKLTIEVWTDGSVKPEDS
                     IGLAAKLIKDHMSIFINFEEDTDDFAYAKMERPPLPRNDQLDRSVDELELSVRSYNCL
                     KNADIRTIRDLVQRTEREMLATKNFGKKSLNEIKEILNGMGLDFGMEFDEQGNPIPGT
                     GGRDGADLSDFDGDDEN"
     gene            complement(1902..2543)
                     /gene="rpsD"
                     /locus_tag="VFH96_02160"
     CDS             complement(1902..2543)
                     /gene="rpsD"
                     /locus_tag="VFH96_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET6972796.1"
                     /translation="MARYRDAVCRLCRREGAKLFLKGDRCYKPACPIEKRGTQPPGQH
                     GNSLRRGKALVGYGQQLREKQKVKRIYFILEGQFRNYFERAARMKGITGENLLFLLER
                     RLDNAVYRAGFSTSRRQARQLVNHGHITVNDRKVDIPSYQVKPGDTIGVREASRKNSH
                     IEGAWQTASGRGRPSWISADGGADMTARVSGAPVRDDIDRTINEQLIVELYSK"
     gene            complement(2684..3082)
                     /gene="rpsK"
                     /locus_tag="VFH96_02165"
     CDS             complement(2684..3082)
                     /gene="rpsK"
                     /locus_tag="VFH96_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET6972797.1"
                     /translation="MAKAGGKKKVFRKKEKKNVPNGIVHIAASFNNTMISITDLEGNL
                     IAQSSAGARGFRGSRKGTPFAAQQAAYEAAKKAHEAGMHQCEVRVKGPGGGRESAIRA
                     IANAGIRVMTIRDTTPIPHNGCRPPKRRRV"
     gene            complement(3108..3491)
                     /gene="rpsM"
                     /locus_tag="VFH96_02170"
     CDS             complement(3108..3491)
                     /gene="rpsM"
                     /locus_tag="VFH96_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET6972798.1"
                     /translation="MARVAGVDLPPNKRAEIGLTYIYGIGRSRSSSVLKEAGVNVDTR
                     VRDLSEDELSRIRAILEAQGEIEGDLRKRVQMDIKRLMDIGCYRGLRHRRSLPVRGQR
                     THTNARTRKGPRRATIAKKKAPGKK"
     gene            complement(3503..3616)
                     /gene="rpmJ"
                     /locus_tag="VFH96_02175"
     CDS             complement(3503..3616)
                     /gene="rpmJ"
                     /locus_tag="VFH96_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET6972799.1"
                     /translation="MKVRASVKKMCDKCKIIHRKGVVRVICANPKHKQRQG"
     gene            complement(3633..3854)
                     /gene="infA"
                     /locus_tag="VFH96_02180"
     CDS             complement(3633..3854)
                     /gene="infA"
                     /locus_tag="VFH96_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET6972800.1"
                     /translation="MAKEEAIEVMAKVLETLPNAMFRVELEDSQHQVLAHISGKMRKH
                     FIRILPGDRVLVELSPYDLNRGRITYRYK"
     gene            complement(4002..4781)
                     /gene="map"
                     /locus_tag="VFH96_02185"
     CDS             complement(4002..4781)
                     /gene="map"
                     /locus_tag="VFH96_02185"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET6972801.1"
                     /translation="MIIGKSKKELEKMRAAGRLVGQVREHLRGMVKPGITTLEIDRVA
                     EKMIRDAGALPTFKGYNGFPYSICASVNEQIVHGFPSHYALQEGDIFSIDCGVTLEGF
                     VGDTATTVPVGEVDEEKLKLIRVTEECLERAIQKCRAGNHLGDIGWAVQEHAEANGYT
                     VVRDYVGHGIGRRMHEDPQIPNYGRPGLGPKIKTGYVFAIEPMVNQGTHFTKVLADGW
                     TVVTVDGKPSAHAEHTIAITEDGPEVLTLISSSAHLEPATV"
     gene            complement(4800..5462)
                     /locus_tag="VFH96_02190"
     CDS             complement(4800..5462)
                     /locus_tag="VFH96_02190"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET6972802.1"
                     /translation="MAKIIVLMGAPGAGKGTQARLLQERLHLPQISTGDIFRALKHAE
                     TPLAQEVRDIMDRGQLVPDELTIQVVKERTAQVDCKNGYILDGFPRTPAQARSLEKLA
                     AEQGNDIVAILIDVPLEMLEKRMTGRRNCPVCNEIYNIYFKPPRYDNVCDLHPEAQLV
                     QRADDNPETVRARLATYEEQTRPLIEYYKAAGLLQTVDGTREPEAIYEDIVRILPRMN
                     TD"
     gene            complement(5469..6887)
                     /gene="secY"
                     /locus_tag="VFH96_02195"
     CDS             complement(5469..6887)
                     /gene="secY"
                     /locus_tag="VFH96_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET6972803.1"
                     /translation="MEKFFAAVRNMFNVPDLRRRIFFTLGLLAVYRFGAHVGAPGINK
                     VRLDEVWRDVAGTLLGVLDLFSGGNFRTISVFALGVTPYITASIILQLMTVVSPQLKK
                     LQEEGEMGRQKINQWTRYLTVVLAGIQTSFVAHWLQVNGVGAPGWGFRLSTVLTLTTG
                     TMFVMWLGEQITERGIGNGISLLIFAGIVIGLPRGVQQVAERLSGGDTMQTIGVIAML
                     AALILIIAFIVYVEAARRKIPVSYAKRHVGRQVVGGQQTTMPLKINMGGVIPVIFASS
                     VLSMPQSLFAAFPPDPANPHTWWAKIYGFFTIFHPGDPYYELIFLSLIIFFTFFYVSI
                     VFNVEEVADNLRKHGGFMPGIRPGRATAEYLWTIMNRLTTVGAIYLALVAFVPQFMLS
                     GFKVGRLPLIGTWLESFVSTTPGLSWIPTGMGYNFYFGGTSLLILVGVAMDTVAQIEA
                     QLVMRNYEGFLGSGGRLRGRRS"
     gene            complement(6915..7364)
                     /gene="rplO"
                     /locus_tag="VFH96_02200"
     CDS             complement(6915..7364)
                     /gene="rplO"
                     /locus_tag="VFH96_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET6972804.1"
                     /translation="MSLNSLKPAQGSTHKKKRVGRGPGSGLGKTAGRGEKGQKSRSGY
                     SRKIGFEGGQMPLHRRLPKRGFTNIFKKQWVEISLAALDQHFEANEEITPEVLHVRGL
                     IKKAKHDVVVLGNGEVSKPLRVSAHRFTKSARQKIEQAGGAVVEIAG"
     gene            complement(7412..7621)
                     /gene="rpmD"
                     /locus_tag="VFH96_02205"
     CDS             complement(7412..7621)
                     /gene="rpmD"
                     /locus_tag="VFH96_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET6972805.1"
                     /translation="MPRRANSNTESQATIRIEYYRSFIHAPKTHKTIVRSLGLRKLHQ
                     VVERPDNDSMRGAVAKVPHLLRIVE"
     gene            complement(7625..8131)
                     /gene="rpsE"
                     /locus_tag="VFH96_02210"
     CDS             complement(7625..8131)
                     /gene="rpsE"
                     /locus_tag="VFH96_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET6972806.1"
                     /translation="MRQPKQRIPAQGLDLKDQVISINRVTKVVKGGKNLSFAALVVVG
                     DEGGHVGFGTGKAREVPLAIKKGVEAAKKNLIRVPLINNTLPHQLIGEYGAGRVLVKP
                     ASEGTGVIAGGAVRAVMQAVGVRDVRTKVLGSTNPHNVVRATFDALLRMKDPMELARL
                     RGKQVEEM"
     gene            complement(8237..8590)
                     /gene="rplR"
                     /locus_tag="VFH96_02215"
                     /pseudo
     CDS             complement(8237..8590)
                     /gene="rplR"
                     /locus_tag="VFH96_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
     gene            complement(8667..9227)
                     /gene="rplF"
                     /locus_tag="VFH96_02220"
     CDS             complement(8667..9227)
                     /gene="rplF"
                     /locus_tag="VFH96_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET6972807.1"
                     /translation="MSRIGKKVIAVPKGVTVTVHERELEVKGPKGTLKTPIPNGISFK
                     VEGEELKAERTSDEQAALHGLARALANNAITGVTEGFSKQMDVVGVGYKADVQGKKIV
                     FSLGYSHPIEFPLPPEVEAKAERLTTKGSIQQYQTTLTLTSIDKQKLGQVAAEMHKLR
                     KPDAYKGKGVRYAGVPIKLKPGKTGK"
     gene            complement(9255..9647)
                     /gene="rpsH"
                     /locus_tag="VFH96_02225"
     CDS             complement(9255..9647)
                     /gene="rpsH"
                     /locus_tag="VFH96_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET6972808.1"
                     /translation="MTDPIADMLTRIRNAIAAKHSRVDIPASKLKLEIARILKEEGYI
                     NNFVIKGEGLKRTVRIFLRYDARGTSSITHLQRISTPGRRVYVGSSDIPKVLGGYGIN
                     IVSTSRGLMSGKAARRENIGGELLAQIY"
     gene            complement(9675..9860)
                     /locus_tag="VFH96_02230"
     CDS             complement(9675..9860)
                     /locus_tag="VFH96_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET6972809.1"
                     /translation="MAKKSLIAKAARKPKFSVRAYTRCKRCGRPRGYLRKFNLCRICF
                     RELALQGQIPGVVKSSW"
     gene            complement(9895..10437)
                     /gene="rplE"
                     /locus_tag="VFH96_02235"
     CDS             complement(9895..10437)
                     /gene="rplE"
                     /locus_tag="VFH96_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET6972810.1"
                     /translation="MAARLKERYQKDVAPQIAKEFGITNPMAVPRIQKVVINMGMGEA
                     IANQKILDTAADELRAITGQKPVITKAKKSIASFKLRQGMPIGVVVTLRGDRMYEFLD
                     RLVSVALPRVRDFRGVSPKAFDGRGNYTIGIREQLIFPEIDFNKVDKLRGMNITIVTT
                     ARNDEQSRALLKGLGMPFRT"
     gene            complement(10465..10800)
                     /gene="rplX"
                     /locus_tag="VFH96_02240"
     CDS             complement(10465..10800)
                     /gene="rplX"
                     /locus_tag="VFH96_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET6972811.1"
                     /translation="MAGTKRSKIKKNDQVAIIAGRDKGRRGRVLEVLPSKGKIKVEGA
                     GMIKRHQKANPQANRGGGIVDKESFIDISNVQLIDPQSGKPTRVRYLVESDGSKTRTA
                     ASSGHALEK"
     gene            complement(10804..11172)
                     /gene="rplN"
                     /locus_tag="VFH96_02245"
     CDS             complement(10804..11172)
                     /gene="rplN"
                     /locus_tag="VFH96_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET6972812.1"
                     /translation="MIQMQTSLEVADNSGARRVEMIMPIGGSTGKIASLGDRIKVTVK
                     EASPDGTIKKGTVQDAVIVRTRKEVRRADGTYVRFDQNAAVLIKEDGTPIGTRVFGPV
                     ARELRDKNFMKIVSLAPEVI"
     gene            complement(11255..11566)
                     /gene="rpsQ"
                     /locus_tag="VFH96_02250"
     CDS             complement(11255..11566)
                     /gene="rpsQ"
                     /locus_tag="VFH96_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET6972813.1"
                     /translation="MAENQENESANAAEPAAAAKSRANRSQKVGTVSSDKMTNTVVVR
                     VDRLVRHRKYRRYVRRTSKFMAHDELGATIGDKVRIVETRPLSARKRWRVIEIVQKAA
                     K"
     gene            complement(11578..11775)
                     /gene="rpmC"
                     /locus_tag="VFH96_02255"
     CDS             complement(11578..11775)
                     /gene="rpmC"
                     /locus_tag="VFH96_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET6972814.1"
                     /translation="MKRREELDKYREMGEDELRAEAARLRESLFRLNFKLALGEVDAV
                     KRIRQEKKALARIQTIASQRS"
     gene            complement(11778..12206)
                     /gene="rplP"
                     /locus_tag="VFH96_02260"
     CDS             complement(11778..12206)
                     /gene="rplP"
                     /locus_tag="VFH96_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET6972815.1"
                     /translation="MLIPRRVKYRKQHHPGRSGHATGGTKVTFGEFGIQALTPAYVTN
                     RQIESARIAMTRHIKRGGKIWIRMFPDKPVTKKPAETRMGKGKGAPEYWVAVVKPGRI
                     LYEIEGVDETTARDAMRLAAQKLPIKTKFVTRADQEGGAI"
BASE COUNT         2440 a         3814 c         3175 g         2910 t
ORIGIN      
        1 tcctccatat tgacggactg cgctggtttt tgcacgttgc gtgtgcgctt cccggttgcc
       61 tcaggctgtt ctttgggtgt ggacattgat taagccacaa agaagcacat aagacacaaa
      121 ttcaggattt gtgtttttgt gcttctttgt ggcgatcctt tgttattcct cgccggactc
      181 agtgccggtg gcctcgtctt cggctgccgc tttcttgtcg cggcctttcc gtttcttcga
      241 cgtcgcggct ttgcgtttct tcgcggcttc ggcagcctcg acctcacgag gattgtcaac
      301 caactcgatg atcgcgagct cggcagcgtc gccgtcgcga tggcccagct tgaaaatgcg
      361 cgtgtaaccg ccgggtcgat ctttataacg atcgccaagc tcggtaaaca gacgtcgcag
      421 cgctgccacc ccggccgtac gctcgggacg cagttgacca cgcttgccag tcacttcctt
      481 gacggtggtg ttgcccgcgt gaaagaaacc cgcggcctgt cgtcgcaagt gcaacgcctg
      541 caggtcagag ccgttgtcag ccaggctgcg ggcccggcgc gacaaagtga tcgcgcgctc
      601 gacaaacggc cgcagttcct tcgccttcgg cagagtcgtg acgatgcgct cctcacgaga
      661 gttgatcaga gaggtcgcta aattgcggag catcgagttc cgatgctcgc tggtgcgacc
      721 gagttttcga tgtgccttta agtgtcgcat gactcaaacc taattttcgt cgtcgccgtc
      781 aaaatcggaa agatcggcgc cgtcacggcc gcccgtgccc ggaatcggat taccctgctc
      841 gtcaaactcc ataccgaagt cgagtcccat gccgttcagg atttccttga tttcgttgag
      901 cgacttcttg ccaaaattct tcgtggccag catctcacgc tcggtgcgtt ggaccagatc
      961 gcgaatggtg cggatgtcgg cattcttcag acagttgtaa gaacgaaccg acagctccag
     1021 ttcgtcaacc gagcggtcca actgatcgtt gcgcgggagg ggcggacgct ccatcttcgc
     1081 gtaagcgaaa tcatccgtgt cttcctcgaa gttgatgaag atcgacatgt ggtccttgat
     1141 caacttcgcc gccagcccga tcgagtcttc cggttttact gaaccatcgg tccagacttc
     1201 gatcgtgagc ttgtcaaatt ccgtgttctg gcccaagcgt gctgcttcga cgttgtagtt
     1261 aaccttcttg acgggggtgt ggaccgaatc gatcggaatg tatccgagcg acagatcctc
     1321 gtcgaagtta cgatcggcgg aaacgtagcc acgtccacgc ttcaagcgca tttcgacttt
     1381 cagcgatcca ccgtcactga cggtcgcgat gtgaacgctc ggatcgagga tctctacgtc
     1441 agcatcggtt tcgatatcgg cagccgtaac ttcgccggct tcctctttat tgatccgcag
     1501 cgtcttgggc tcgctggtgt gtaacttaaa aggtacttgc ttgagattga gaataacgtc
     1561 ggtggcgtct tccaccaccc ctttaataga tgaaaactca tgctcgacgc cttcaatctt
     1621 gacggccgtg atagcggcgc cttcgattga agaaagcaac gcccgacgga gcgaattacc
     1681 gaccgtcgtt ccaaagcctc gttcgaatgg ctgcgctgaa aagcggccat atcgttcggt
     1741 gagggtctct gcgtctgccg caagtcgtcg cggcatttga aatcctgtcc aaagattatt
     1801 ttgatccata gccatagcgt tcaggttcct aagaaaaatg catcggacca ggcagtgacg
     1861 atcggtgcac gttggccgaa gtcactgcct gctcgggtta attacttact ataaagctca
     1921 acaatcaact gctcgttgat cgtgcgatcg atatcgtcac gcaccggcgc gccgcttacg
     1981 cgggcagtca tgtcggcgcc gccgtctgcg gagatccacg acgggcgtcc acgaccagac
     2041 gcggtttgcc acgcaccctc gatatgagag ttctttctgc ttgcctctcg cacaccgatg
     2101 gtgtcgcccg gcttcacttg ataagaggga atatctactt tgcgatcgtt gaccgtaatg
     2161 tgaccgtggt taacgagctg tcgcgcctgc cggcgtgagg tcgaaaagcc ggcgcgataa
     2221 acggcattgt ccaggcgccg ctcgagcagg aacaaaaggt tctcgccggt aatacccttc
     2281 atgcgcgcgg cgcgttcgaa gtagtttcga aactggcctt cgagaatgaa gtagatgcgt
     2341 tttaccttct gcttctctct gagctgctgg ccatagccga ccagcgcttt gccgcggcgc
     2401 agcgaattac cgtgctgacc cggcggttgc gtaccacgtt tttcaatcgg gcaagcgggt
     2461 ttgtaacaac gatcgccctt caaaaacagc ttcgcgcctt cgcgacggca aaggcgacac
     2521 acagcatctc tatacctagc caattatttg cctccggtct attgcctcca acctgatgat
     2581 caggttggaa aagtttacag atcccgcgat aatgctggat ccttcttcct ttgggtttat
     2641 cgaataggac caatagggac ctattcgacc tattaaaata aaactaaact cttctacgct
     2701 tcggcggacg gcagccgttg tgcggaatcg gtgtcgtatc gcgaatcgtc atcacgcgga
     2761 tacccgcatt cgcgatcgcg cggatcgccg actcacgacc accgcctggt cctttcacac
     2821 gcacttcaca ctggtgcatg cccgcttcgt gcgccttctt cgctgcttcg taagccgcct
     2881 gctgggccgc gaacggcgtc cctttacgcg agccacggaa gccacgcgcg ccggctgacg
     2941 attgcgcgat caggttcccc tcgagatccg tgatcgagat catcgtgtta ttgaacgacg
     3001 ccgcgatatg cacgatgccg ttgggaacat tcttcttctc tttctttcga aagaccttct
     3061 tttttccgcc tgctttagcc ataacttttc agtcaaacaa tcaaaaacta cttcttgccg
     3121 ggcgccttct tcttggcgat ggtggcacgt cgcggacctt tgcgcgtgcg cgcgttggta
     3181 tgagttcgct gaccgcgcac cggcaacgaa cggcgatgcc tgagaccacg atagcaaccg
     3241 atatccatca agcgcttgat atccatctgg acgcgcttgc gcaggtcgcc ttcgatctct
     3301 ccctgcgctt ccagaatggc acggatgcgg ctcagctcgt cctcactcag atcgcgcacg
     3361 cgagtgtcga cgttgacgcc cgcttccttc agcacagaac tcgaacgcga gcggccaatg
     3421 ccgtagatgt aagtcagacc aatctcggct ctcttattgg gcggtaaatc tactcctgct
     3481 acacgtgcca ttgtatttct cgttatcctt gtctctgttt atgtttggga ttcgcacaaa
     3541 tcacacgcac cactcctttg cggtggatga tcttgcactt gtcgcacatc tttttcactg
     3601 acgcacggac tttcattacg taacctcgtt aactatttat agcgataagt gatgcggccg
     3661 cgattgagat cgtacggcga aagctccact aaaaccctgt cacccggaag tattcgaata
     3721 aaatgcttgc gcatcttccc tgatatgtgc gcaagcacct gatgctggct gtcttccaac
     3781 tcgacccgaa acatagcgtt aggcaaagtc tccagcacct ttgccatgac ttctatcgct
     3841 tcttccttcg ccatatcctc tctgggtgga attacgacac tgccgacaaa ctgcgaataa
     3901 tagcacgcag gttaattctt tgccaagctg aggtgtccga taaactttag tttgtcggga
     3961 catttcgaca agctaaagct tatcggacat caaatccgcg gctaaactgt tgccggctcg
     4021 aggtgtgcgc ttgaggaaat aagcgtcaac acttccggac catcttccgt aatcgcgatg
     4081 gtgtgctcgg cgtgggcgct cggcttccca tcgaccgtca caaccgtcca tccatccgct
     4141 aaaaccttcg taaaatgcgt tccctggttg accatcggct cgattgcgaa cacgtaaccg
     4201 gtcttgatct tcggtcccag gcctggccgg ccatagttcg gaatctgcgg atcctcgtgc
     4261 atccggcgcc cgattccgtg cccgacatag tcgcgcacca ccgtataccc gttcgcctca
     4321 gcgtgctcct gcacggccca accgatgtca ccgaggtgat ttcccgcccg gcacttctgg
     4381 atcgcacgct ccaggcattc ctcggtcaca cggatcagct tgagcttctc ctcatcgact
     4441 tcgccgaccg gaaccgtcgt ggctgtatcg cccacaaaac cctcgagcgt gaccccacag
     4501 tctatcgaga agatatcgcc ttcctgcaaa gcataatgcg acggaaaacc gtgcacgatc
     4561 tgttcattga ccgaggcaca gatcgaatac ggaaacccgt tataaccctt gaacgtcggc
     4621 agcgcaccgg catcacgaat catcttctcc gcgacccgat ctatctcgag cgtcgtgatc
     4681 cccggcttga ccatcccgcg caaatgctcc cgcacctgcc cgaccaggcg tcccgccgcc
     4741 cgcatcttct ccagctcttt ttttgacttc ccaataatca ttgcaaaaaa tccgcgtttt
     4801 taatccgtgt tcatccgcgg cagtattctg acgatgtcct catagatcgc ttccggctct
     4861 ctcgttccgt ccaccgtttg cagcaatccc gccgccttgt agtactcgat taacggtcgc
     4921 gtttgttcct cgtacgttgc gagtcgcgcg cgcaccgtct ccggattgtc gtccgcgcgc
     4981 tgcacgagtt gtgcttccgg atgcaaatca caaacgttgt cgtagcgcgg cggcttgaaa
     5041 tagatattgt aaatctcgtt gcaaaccggg caattgcgac gtccggtcat ccgcttttcg
     5101 agcatttcca gcggcacatc gatcagtatc gcgacgatat cgttgccctg ctcggcggcc
     5161 agcttctcaa gactgcgcgc ctgtgcgggc gtgcgcggaa aaccgtcgag aatgtagccg
     5221 ttcttacaat cgacctgcgc cgtgcgctct ttgaccactt gaattgtcaa ctcatcggga
     5281 accagttgtc cccggtccat gatatcgcga acttcctgcg cgaggggcgt ctcagcgtgt
     5341 ttgagcgcgc gaaaaatatc ccccgtcgag atctgaggca aatgcaaccg ttcttgaagc
     5401 aagcgcgcct gcgtgccttt tccggcacca ggcgcaccca tcaacactat gatttttgcc
     5461 ataaaggatc aggagcgccg cccgcgcagg cgaccgccac tgcccagaaa tccttcgtag
     5521 ttgcgcatca ccagctgcgc ttcgatctga gcgaccgtgt ccatcgcgac gccgacaagg
     5581 atcagcagcg acgtaccgcc aaaatagaag ttgtagccca tgcccgtcgg gatccatgag
     5641 agaccgggcg tcgtactgac aaacgattcc agccacgtgc cgatcagcgg taagcgaccg
     5701 accttgaaac cactcaacat gaactgcggc acgaacgcga cgagggccag ataaatagca
     5761 ccgacagtcg tcaaacgatt catgatcgtc cagagatact cagccgtcgc ccgcccggga
     5821 cgaatacccg gcatgaaccc gccgtgcttg cgcaggttgt cagccacctc ctcgacgttg
     5881 aagacgatcg atacgtaaaa gaacgtaaag aagatgatca gcgacaggaa aatcaactcg
     5941 taatacggat cgcccggatg gaagatcgtg aagaagccgt aaatctttgc ccaccacgta
     6001 tgcggattag ccggatccgg aggaaacgct gcaaagaggc tctgcggcat cgacaaaact
     6061 gacgacgcaa agatcacggg gatcacgccg cccatattga tcttcagcgg catcgtcgtt
     6121 tgctgtccac cgactacctg tcgaccaacg tgtcgtttcg cgtaactgac cggaatctta
     6181 cgtcgcgccg cttcgacata aacaatgaac gcaatgatca aaattaaggc cgcgagcatg
     6241 gcgatcacgc cgatcgtctg cattgtgtcg ccgccgctga gtcgctccgc cacctgctgc
     6301 acaccacgcg gcagaccaat cacgataccc gcgaagatca gcaacgagat accgttgccg
     6361 ataccgcgct cagtgatctg ctcgcccagc cacatgacga acatcgtgcc ggtggtcaga
     6421 gtcagaaccg tgctgagacg gaatccccag ccgggtgcgc cgacgccgtt gacttgcagc
     6481 cagtgcgcca cgaacgaagt ctggatccca gcgagcacaa ccgtcaggta gcgcgtccac
     6541 tggttgatct tctgacggcc catctcgccc tcttcctgca actttttgag ctgcggcgag
     6601 accaccgtca tgagctgaag aatgattgac gcggtgatgt aaggagtaac gcctaaagcg
     6661 aagactgaaa tcgtgcggaa gtttccgccg gagaagaggt cgagcacacc gagcagcgtt
     6721 cccgccacgt cgcgccaaac ttcatcgagg cgcaccttgt tgataccggg cgcgccgacg
     6781 tgcgcgccaa aacggtaaac agcaagcaga ccgagtgtaa agaaaatgcg ccggcgcaga
     6841 tccggcacat tgaacatatt gcgaaccgcg gcaaaaaact tttccattac tgttctcctg
     6901 ccggcgatcc caggctatcc ggcaatctcg actactgcac cgcctgcctg ctcgatcttt
     6961 tgacgagcac tcttggtgaa ccggtgggct gaaacacgaa gcggttttga gacttcgccg
     7021 tttccgagca ccaccacgtc gtgcttggct ttcttgatca gcccgcgaac gtgcaacact
     7081 tccggagtga tctcttcgtt cgcttcgaaa tgctgatcga gagcggcgag actaatctcg
     7141 acccactgct tcttaaagat attggtgaaa ccgcgcttcg gcaagcgccg gtgcaggggc
     7201 atctggccgc cttcaaaacc gatcttgcgc gaataacccg aacgagactt ctgtcccttc
     7261 tcaccgcggc ccgcggtctt gcccagacca gagcctggcc cacgcccgac acgcttcttc
     7321 ttgtgggtcg aaccctgcgc cggctttaga ctattaagac taatcgccat ctcttactcc
     7381 cttataggtc ctatacgtcc tataggtcct attattcaac tatccttaac aggtgcggca
     7441 ctttcgcgac cgcgccacgc atcgagtcgt tgtccgggcg ctcgacgacc tggtgcaact
     7501 ttcgcaaacc cagactgcgc acgattgtct tgtgtgtctt cggcgcatga ataaaactcc
     7561 ggtagtactc gatacgaatt gtcgcttgtg attctgtgtt gctgtttgct ctacgcggca
     7621 taccttacat ctcctcaacc tgcttgccac gcaagcgcgc cagctccatc ggatccttca
     7681 tgcgcagcaa cgcatcgaaa gtcgcacgca ccacgttgtg cggattcgtc gagcccagca
     7741 ctttcgtgcg cacgtccctg acgccgactg cctgcatgac cgcacgcacc gcgccgcccg
     7801 cgataactcc agtaccttcc gaagcaggct taacgagcac acgacccgca ccgtattcac
     7861 caatcaactg gtgcggcaaa gtgttattga tcagcgggac gcgaatcaga ttcttcttcg
     7921 cggcctcgac tccctttttg atcgcgaggg gcacttcgcg cgccttgccg gtgccgaaac
     7981 cgacgtgccc gccttcatca ccaaccacta cgagcgccgc aaacgagagg ttcttaccgc
     8041 ccttaacgac tttggtgacg cgattgatgg agattacctg atctttcagg tcgagcccct
     8101 gcgcgggaat gcgttgcttg ggttgtctca tagtctattc ttgtttcttc ttcttcgact
     8161 tcttttcttt tggctcggcg acttccgcca ccggctcttc tgccgctgct tcgggctcgt
     8221 cgacgacttc gttcttgttc aagcccgctg cccgcgccgc gtccgtcaaa gccttgatac
     8281 ggccgtgata gagatagccg ccgcgatcga aaacgacctg ctcgattccc ttcgcgagcg
     8341 cgcgctcggc aatcgcctgg ccgatacggc gggccgcatc gagattaccg ccagtgccgg
     8401 tacgcagatc cttctcggtg gacgacgccg aaacgagcgt ctggcccata cggtcgtcga
     8461 tcacctgcgc ggagatgtgt ttcaggctgc gatagatcgc gagtcgcggt ctctccgtgc
     8521 ttccttttac tttgcggcga atgcgcgagt gaatcgcggt tcgcacttct gctctatttg
     8581 tgtttgccat ctcgaatacc ttatccaccg attacgcaga ctacgcagat tttttatcgc
     8641 ctctgtgtaa tctgtggacg actacactat tttccggtct tgcccggctt caacttaatc
     8701 ggcacacccg cgtaacgcac gcccttgcct ttgtaagcgt cgggcttgcg aagcttgtgc
     8761 atctcggctg cgacctggcc cagcttctgc ttgtcgatac tggtcaaggt caacgtcgtc
     8821 tgatactgct gaatacttcc cttcgtcgtc agacgctcag ccttcgcctc gacttcaggc
     8881 ggcaggggaa actcgatcgg gtgcgaataa ccgagcgaga agacgatctt cttgccctgc
     8941 acgtcggcct tgtaaccgac cccgaccacg tccatctgct tgctgaagcc ttcggtcacg
     9001 ccggtgatgg cgttgttggc gagcgcccgc gccagaccat gcagggccgc ctgctcgtcc
     9061 gaagtacgct cagccttcag ctcttcgcct tcgaccttga agctgatccc gttcggaatc
     9121 ggagtcttca acgtgccctt cggccctttg acttcgagct cgcgctcgtg aacggtgacg
     9181 gtcacgccct tcggcaccgc gattaccttt tttccaattc gtgacataac gtacagtcct
     9241 gtttagtaga gcgtctaata gatctgggcc agcagctcgc cgccgatatt ctcgcgtcgc
     9301 gctgccttac cgctcatcaa acctcttgac gtggagacga tgttgatacc gtagccaccg
     9361 agcaccttcg gaatatcact cgagccgaca tacacgcgcc gtccgggagt cgagatacgc
     9421 tgcaggtgcg tgattgaaga cgtgccgcgc gcgtcgtaac gcaggaagat acgcacggtg
     9481 cgcttgagcc cttcgccctt gatgacgaag ttgttgatgt aaccctcttc cttcagaatg
     9541 cgggcaatct cgagtttgag ctttgaagcc ggaatatcta cacgcgaatg cttcgccgca
     9601 atggcgttgc ggattcgcgt caacatatcg gcaataggat cagtcatgaa ttaactccgt
     9661 caattaattg ccagttacca actcgactta acaacgccgg ggatctgtcc ctgcagcgcg
     9721 agttcacgga aacagatgcg acacaggttg aacttccgca aataacctcg cggccgtcca
     9781 cagcgtttac atcgagtgta ggcgcgcacc gaaaacttcg gtttgcgtgc cgccttcgca
     9841 atcagtgatt tctttgccat aagatcttct ttgttatagg ccctataggt cctattaagt
     9901 tctaaagggc attcccaggc ccttcaaaag cgctcgcgac tgctcatcgt tgcgcgccgt
     9961 cgtcacgatc gtgatattca tcccgcgcag cttgtcgacc ttgttgaaat cgatctccgg
    10021 gaagatcaac tgctcgcgaa ttccaatcgt gtagttgcca cgaccatcga aagccttggg
    10081 cgacacgccg cgaaagtcgc gcacgcgcgg gagggccacc gacacgagcc ggtcgagaaa
    10141 ctcatacatc cggtcgccac gcaaagtaac gaccacaccg atcggcattc cctgtcgcag
    10201 cttgaacgac gcgatcgact tcttcgcttt cgtgatcacg ggcttctgac ccgtaatcgc
    10261 gcgcagctca tcagccgcgg tgtcgagtat tttctggttg gcaatcgcct cgcccatgcc
    10321 catgttgatt acgaccttct gaatacgcgg cacagccatg ggattagtga tgccaaactc
    10381 cttggcaatc tgcggcgcca cgtctttttg atatcgctct tttaaccttg cagccatgtc
    10441 agatcctcga attttacttc tctactactt ttccaacgcg tggccgctgg aggccgccgt
    10501 cctcgtcttc gaaccatcgc tctcaaccag atagcgcacg cgcgtcggct tgcctgactg
    10561 cggatcgatc aactgcacgt tcgagatgtc gataaaagat tccttatcga cgatgccgcc
    10621 accgcgattc gcctgcgggt tcgccttctg gtgacgcttg atcatgcccg cgccttccac
    10681 tttgatcttg cccttgctcg gcaacacttc cagcacgcga ccacgcctgc ctttgtcgcg
    10741 cccggcaatg atcgctacct ggtcgttctt cttgatcttg gagcgctttg ttcctgccat
    10801 ctcttaaatc acctccggcg cgagcgaaac gatcttcatg aagttcttgt cgcgcagctc
    10861 acgcgctact ggtccaaaca cgcgcgtacc gatgggcgtg ccgtcttcct tgatcagcac
    10921 cgcggcgttc tgatcgaacc tgacgtacgt tccgtcagcg cggcgcactt ccttgcgcgt
    10981 ccgcacgatc accgcgtcct ggaccgtgcc cttttttatc gtgccgtccg gcgaagcctc
    11041 tttgaccgtc accttgatgc ggtctcccag actcgcaatc tttcccgtgg agccgccgat
    11101 cggcatgatc atctccacgc gtcgcgcccc tgaattgtca gcaacctcca gagaggtttg
    11161 catctggatc actgtcgtta cctccgacta ttgccttgaa gccatccgca gatttcacag
    11221 atttttcttc tgcgtaatct gcgcaatctg tggactactt cgcagctttc tgaacgatct
    11281 caattacacg ccagcgcttg cgcgcggaaa gtggacgcgt ttcaactatg cgcaccttgt
    11341 cgccaatggt cgcgccgagc tcgtcgtgcg ccatgaactt cgacgtgcga cgcacgtagc
    11401 ggcgatactt gcggtgccgg accaggcggt ccacccgaac cacgacggta ttcgtcatct
    11461 tgtcagacga cacggtcccc accttctgcg atcgattggc acggctcttc gcggcggctg
    11521 ctggttcggc agcgtttgcg ctctcgtttt cttgattctc tgccattctc tttagtccta
    11581 acttcgctgg ctcgcgatcg tctggatccg cgccaacgct ttcttttcct ggcgaatgcg
    11641 cttgactgca tcaacctcgc cgagcgcgag tttgaaattc aagcgaaaca acgactcacg
    11701 caaacgcgcc gcttcggcgc gcagctcgtc ctcgcccatc tcgcgatact tgtcgagctc
    11761 ttctcttctc ttcatcgtta gatcgcgccc ccttcctgat cggcgcgggt cacaaacttc
    11821 gtcttgatcg gaagcttctg ggccgcgagc cgcatcgcgt cgcgcgcggt cgtctcatcg
    11881 acgccttcga tctcgtaaag aattcgcccc ggcttgacca ctgctaccca gtattccggc
    11941 gcgcccttgc ctttgcccat acgtgtttcc gcgggcttct tcgtcaccgg cttatcggga
    12001 aacatgcgaa tccagatctt gccgccacgc ttgatgtgac gcgtcatcgc gatacgagcg
    12061 gactcgatct gacggttggt cacgtacgcg ggggtcaggg cctggatgcc gaactcgccg
    12121 aaggtgacct tcgtgccgcc ggtggcgtgg cccgaacggc cggggtggtg ctgcttgcgg
    12181 tacttgactc gacggggaat caacatggtt atgcctcaac tcctgctgcg acgggcgctg
    12241 cctcgggcgc gttggtacgc gcgccgcgac gcggacggtc gctgttccgc tcggggcgga
    12301 ccgacttggt gttggcctgc tcgcgagcga gttccttgt
//
LOCUS       DASSFQ010000070         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1670675, whole genome shotgun sequence.
ACCESSION   DASSFQ010000070 DASSFQ010000000
VERSION     DASSFQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670675"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFH96_02265"
     CDS             complement(<1..415)
                     /locus_tag="VFH96_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972816.1"
                     /translation="MNTFWQDIRYGARTLMKNPGVTIIVIIALALGIGANTAIFSVVN
                     TVLIRPLPYEESDRLVFLNEKSPLLDEMSISYPNFLDWREHNQTFEKMGVYNRASYNL
                     TGAGEAERIVTGQISADLFSVLRVNPLLGRVFTNDE"
     gene            complement(478..>2277)
                     /locus_tag="VFH96_02270"
     CDS             complement(478..>2277)
                     /locus_tag="VFH96_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6972817.1"
                     /translation="RARFDLITRDMQAAYPEEWLSSSESGRRRVSAITVLPESETRLH
                     PDMQSAAYGVFALVFVIVNLVLLIACINLAGMLLARAVTRRREMAVRLAIGASRSRIV
                     RQLLTESVLLSIIAGVAGILLAIWFLNAIVAAIPALPEGMRVAVDLHLDWRVVIYTIA
                     FSTITGILFGLAPALQSSKADVSTVLKDDSSLFTGFYRKSRTRMALVVVQVAFSLLLL
                     VGAGLVLRSLEKIRPSRLGFSTESMVVGLVRLDEAKYDRAKTQEFYRQLSERLAALPG
                     VQSASLVSEIPLSFMGGSRSSIEIEGYQPGANEDMQIAAVIAGPRYFTNMKVPFMQGR
                     DFEERDREGAPCVALVNEAFGEKYFRGSGSLGKHLMKYGGAENAPRIPCEVVGVIRDN
                     EWQSLEKQVHPFYALALQQSQRKQFMLVANAVGDPASLIGGVRNTIRELDPMIPVADV
                     QTLGDYFSLGLFPFRILAVVMGGCGLMALLLATLGIYGIISYSVVQRTRELGIRMALG
                     AVQRDILRMVIGQGMFLVLVGLGIGLLLSLALTRVLTSSLLELELPLPVSAIDPLTFA
                     GVTVLLAFVALAACYIPARRATKVDPIEALRYE"
BASE COUNT          552 a          711 c          569 g          445 t
ORIGIN      
        1 cttcatcgtt cgtaaacacg cgaccgagta gcgggttcac gcgcagtaca gaaaatagat
       61 cggcagaaat ctgaccggtg acgatgcgtt ccgcttcgcc cgcgccggtg aggttgtaac
      121 tggcgcggtt atagacgccc atcttctcga acgtttgatt gtgctcgcgc cagtcgagaa
      181 agttcggata cgagatcgac atctcatcga gcaacggact cttctcgttg agaaagacga
      241 gccgatcaga ctcttcgtaa ggcaacggac gaataagcac ggtattgacg acactgaaga
      301 tcgcggtgtt cgcaccgata ccgagcgcga gcgcaatgat cacgatgatc gtaacgcccg
      361 ggttcttcat caacgtcctc gcgccatagc gtatgtcctg ccagaatgta ttcatcgatt
      421 ctccttatcc acagattaca cagattattt tctgcgtaat ctgtgtatct gtggacccta
      481 ttcgtatctt aatgcttcga tcggatctac tttcgttgcc ctgcgcgcgg gaatgtaaca
      541 agccgcgagg gccacgaacg ccagcaacac cgtcacacct gcaaacgtca gcggatcgat
      601 cgcactgaca ggcagcggca gctcaagctc taacaacgag ctcgtcaaca ctcgcgtcaa
      661 cgccaacgac aacaacaatc cgattcccaa cccaacaagc acgagaaaca ttccctgccc
      721 gatcaccatc cgcaaaatat ccctttgcac cgcgccgagc gccatgcgaa tgccaagctc
      781 gcgcgtgcgc tggaccactg aataagaaat gattccataa atcccgagcg tcgccagcaa
      841 cagggccatc agcccgcagc cacccatcac gaccgccagg atgcgaaacg gaaaaagccc
      901 gaggctgaag taatcaccca acgtctgcac gtcagccacc ggaatcatcg gatcgagctc
      961 gcggatcgtg ttacgcactc caccgattaa acttgccgga tcgccaacag cgttcgccac
     1021 cagcatgaac tgttttcgtt gagactgttg caacgcgagc gcgtagaacg gatgcacctg
     1081 cttctccagc gattgccact cgttgtcgcg tatcacgccg acgacctcgc acggtattcg
     1141 cggagcattc tctgcgcccc cgtatttcat gaggtgcttc ccgagcgagc cggatccacg
     1201 aaagtatttt tcgccgaacg cctcattcac caacgccaca cacggcgcgc cttcacgatc
     1261 gcgttcctcg aaatcgcgtc cctgcatgaa cgggactttc atattcgtga aataacgcgg
     1321 gcccgcgatg acggcagcga tttgcatgtc ctcatttgcg ccgggctgat atccctcgat
     1381 ctcgatgctg ctccgcgagc cacccatgaa cgataacggt atctcactca ctaaactcgc
     1441 cgattgtacg cccggcaaag cagcaagccg ctccgacagt tgccggtaaa actcctgcgt
     1501 cttcgcgcga tcgtatttag cttcgtcgag ccgcacgagt ccaacgacca tactttccgt
     1561 gctaaagccc aaacgtgacg gtctgatctt ttcaagactg cgcaacacga gtcctgcgcc
     1621 aaccaaaagc agtaacgaaa aggcgacctg gaccaccacc agggccatgc gagtgcgcga
     1681 cttgcgatag aagccggtga acagactcga gtcgtctttc aacacagtcg aaacgtctgc
     1741 tttcgagctt tgcaatgcag gtgcgagtcc aaaaagtatt cccgtgattg tcgaaaacgc
     1801 gatcgtgtag atcacgacgc gccagtcgag atgaagatcg acggcgacgc gcataccttc
     1861 cggaagtgca gggatcgcgg cgacgatcgc attgagaaac caaatagcca gcagtattcc
     1921 ggctacgcct gcgatgatcg acagcaacac actttcagtc agcaactgtc gcacgatccg
     1981 cgaacgactc gcgccgatcg caagccgcac ggccatctct cgtcgtcgtg tgacggctcg
     2041 cgccagcaac atgcccgcga gattgatgca cgcgattagc agcacgaggt tcacgatgac
     2101 aaagaccaga gcaaacacgc cgtacgcggc tgactgcatg tctggatgaa gccgcgtctc
     2161 actctcaggc agcaccgtga tcgccgacac acgcctgcgt cccgattcgc tgctcgacag
     2221 ccactcttcc ggatatgcag cctgcatgtc gcgcgtgatc agatcgaaac gtgcgcg
//
LOCUS       DASSFQ010000071         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1520101, whole genome shotgun sequence.
ACCESSION   DASSFQ010000071 DASSFQ010000000
VERSION     DASSFQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520101"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFH96_02275"
     CDS             <1..576
                     /locus_tag="VFH96_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972818.1"
                     /translation="YKNSVPIGYFEGISLFERMESGFNLYYTFRDGETAWLYARTLNV
                     FHHALGVRAFSLDPYQIGHENEEGIESGAFWFYRKLGFRPTSKDVLRIALKEEEKIKT
                     REGYRTSPATLRKLAQSPMIFELDRERVGDWDRFQIRHVGFKAQRRLSAEKEQALAQI
                     AKAKASREEATYLRTIQRDEKLRSVIIKLGS"
     gene            complement(573..1484)
                     /gene="ftcD"
                     /locus_tag="VFH96_02280"
     CDS             complement(573..1484)
                     /gene="ftcD"
                     /locus_tag="VFH96_02280"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET6972819.1"
                     /translation="MQPRLVECIPNFSEGRRPEIVHQIAEAIAGIKPACVLDTHIDPD
                     HNRSVITFVAPPDKVVDAAVSAVRRASELIDMRTHRGEHPRLGATDVLPFVPLDGVSM
                     DECVRLAHEAGTIIARDLSIPVYYYERAALRPERVNLEGVRRGALELLREQITTNPDR
                     IPDAGLTRVHESAGAIVVGARSFLIAFNVILRSSDIAVARQIARKIRASNGGLPYLKA
                     LGFSLRTRELVQVSMNLINYEVTGMAAVYDAVKGEADLLGVEIESAEIVGLVPRNALD
                     RNAAYFSKLENFSESKILEHQIREICG"
     gene            1531..2493
                     /locus_tag="VFH96_02285"
     CDS             1531..2493
                     /locus_tag="VFH96_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET6972820.1"
                     /translation="MPPDRFINKRKNTWQRLEDLLKLLDSSSLGRLNRDEVRELGRIY
                     RRTASDLAIARAESRDPRLVNYLNSLVIRAHGRIYRANAQGGKRIQRFFTHELPQTFR
                     RTWRYTLLSFSIFAIFATFSFVATRYDPEFSELVGVPPTFRETYIETKTHWWERLNES
                     NQEGASMIMTHNILVTIYTFAFGATFGIGTLFYLAFNGANIASVLALTYRAGFGNDLV
                     TFMVAHGVVELSCIFIAGGAGLLIGSAMIMPGNLTRADALKTRGMEAVRLMIGVAILL
                     VLAGTIEGFISPVPMDARIKYSIAIVTGVALYSYLLLVGHERAE"
BASE COUNT          587 a          722 c          726 g          585 t
ORIGIN      
        1 tacaagaaca gcgtgccgat tggttacttc gaaggcattt cgctgtttga acgcatggag
       61 agtgggttca acttgtacta cacgtttcgc gacggcgaaa cggcgtggct gtacgcgcga
      121 acactcaacg tttttcacca tgctctcggc gtgcgcgcgt tctcgctcga tccatatcaa
      181 atcggacatg aaaacgagga aggcattgaa tcaggcgcgt tctggtttta tcgcaagctg
      241 ggtttcagac cgacaagcaa agacgttttg agaatcgcgc tcaaggaaga ggaaaagatc
      301 aaaacgcgtg aaggctatcg cacatcacct gcgacgctgc gtaagctcgc ccagagtcca
      361 atgatcttcg agcttgatcg cgagcgcgtt ggcgactggg atcgattcca gatccggcat
      421 gtcggcttta aggcacaacg ccggttgagt gcggaaaaag agcaggctct tgcacaaatt
      481 gctaaagcca aagcgagtcg tgaagaagca acgtatctca gaacgattca acgagatgaa
      541 aaactgcgaa gcgtgatcat taagctcgga tcttagccac agatttcacg gatctgatgt
      601 tcgaggatct tcgattcaga gaagttttcg agttttgaga aataagctgc gtttcgatcg
      661 agagcgtttc gcgggacaag accgacgatt tcggcgcttt cgatttccac acccaacaga
      721 tctgcttcgc cttttaccgc atcgtacacc gcagccattc ctgttacttc ataattgatc
      781 agattcatcg acacctggac caactcacgg gttcgcaaac taaagccgag tgccttgagg
      841 taaggcaaac cgccgttgct cgctcggatt ttgcgtgcga tctgtcgcgc aacggcaatg
      901 tcggaactac gcagaatgac gttgaaagcg atcagaaacg atcgcgcgcc aaccacgatc
      961 gcaccggctg attcgtgaac gcgagtcaac cccgcgtctg gtatgcgatc gggattggtt
     1021 gtgatctgtt cgcgcaacaa ctccaacgca ccacggcgaa caccttccag attcactcgt
     1081 tcgggacgca gcgccgctcg ctcgtaatag tagacgggga tcgaaagatc gcgtgcgatg
     1141 attgttccgg cttcgtgagc gagcctgaca cattcgtcca tcgagactcc gtcgaggggc
     1201 acaaaaggca gcacgtcagt ggcgccgagg cgcggatgct cgccccggtg cgtgcgcatg
     1261 tcaatcaatt ctgaagctcg cctcacggcg ctcactgccg cgtcaactac cttgtccggt
     1321 ggcgcgacaa aagtgattac cgagcgattg tgatcggggt cgatatgcgt gtcgagcacg
     1381 catgccggtt tgatcccggc gatcgcctca gcgatctggt gcacaatttc gggccggcgg
     1441 ccttcgctga agttggggat gcactcgacg agtcgtggct gcatacagat atcttagaca
     1501 ggattgacag gattttgtat cttcttcaat gtgcctccgg atcgattcat aaacaaacgg
     1561 aaaaacacct ggcagcggct cgaggatctg ctgaagttac tcgacagctc ctcgcttggt
     1621 cgtttgaacc gcgatgaggt ccgggagctc ggacggatct accgtcgcac ggcttcagat
     1681 ctcgccatcg cccgcgctga atcacgcgat ccgcgtctgg ttaattacct caacagtctg
     1741 gtaattcgcg ctcatggccg catttatcgc gccaatgcgc agggtggcaa acgcatccag
     1801 cgattcttca cccatgaatt gccgcagacg ttccggcgta cctggcgcta cacgctgctc
     1861 tcgttctcaa tctttgcgat cttcgcgacc ttcagctttg tagcaacgcg atacgatccc
     1921 gagttttccg aacttgtcgg cgtacccccg acatttcgcg aaacgtacat cgaaaccaaa
     1981 acgcactggt gggaacgact caacgagtcg aaccaggaag gcgcgagcat gatcatgacc
     2041 cacaacattc tggtcacgat ctacaccttt gcgttcggcg ccactttcgg aatcggtacg
     2101 ctcttttatc tcgccttcaa cggcgccaac attgcttcgg tgctggcgct aacttaccgc
     2161 gccggatttg gtaacgacct ggtgacgttt atggttgcgc acggagtggt tgagctgtcg
     2221 tgcatcttca tcgccggtgg cgctggtctc ctgataggta gtgcgatgat catgcccggc
     2281 aatctaacgc gcgccgacgc actcaagact cgcggcatgg aagcggtgcg gctgatgatt
     2341 ggggttgcga ttctgctcgt gctcgcgggc acgattgaag gcttcatctc gcctgtgccg
     2401 atggatgcga gaatcaagta cagcatcgcg atcgtcacgg gcgtggcgct gtattcctat
     2461 ctgctccttg tcggacacga aagagctgaa tagcgaagat gacgccatcg aacgtactgt
     2521 aggggcggcc ctccgtggcc gcccctgcgt ggaactcatc ggattcaaaa aggcaacgat
     2581 gttcggctcg gcgaacacga ttcgacgcag gggcggccac
//
LOCUS       DASSFQ010000072         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_28884, whole genome shotgun sequence.
ACCESSION   DASSFQ010000072 DASSFQ010000000
VERSION     DASSFQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28884"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFH96_02290"
     CDS             <1..477
                     /locus_tag="VFH96_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972821.1"
                     /translation="LRLAYNEKKKYLGEEELDPLEFKDLLKERAKVVEMDASFMTRSV
                     NENFSGGEKKRNEILQMAVLEPKLAVLDETDSGLDIDALRIVANGVNQLFTPENAIIV
                     VTHYQRLLNYIVPHFVHVLADGRIAREGGKELALELEAKGYDWIRTADKGAGVPAN"
     gene            480..1799
                     /gene="sufD"
                     /locus_tag="VFH96_02295"
     CDS             480..1799
                     /gene="sufD"
                     /locus_tag="VFH96_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET6972822.1"
                     /translation="MVTELVKSENSYQGAFRSARPTVAWLELVRNSAMDRFEQLGFPS
                     VKEEDWKYTNLATLAKEEFVPVTTGTTDVGEVEGYPETAETHLVVVDGFLREDLSTKT
                     GLGDVVEIDLFNAVEDARYNKIVRKYLARNAGYHNNGLTALNTAFLQSGVFLWIPKNV
                     KLERPLQITFVADAEHGASFPRLLVVAEENSSATLIESFVSSNGGKYFTNATAEIVLK
                     DGAQLEHYRVQRESHNAYHVSTTSAELGRASRYYTTSINLGAQLSRHDVSVVMDHEGA
                     ETAVDGLYMVGSDQHSDTHSVIDHKQPHCTSHQLYKGILDGNGRAVFNGKVFVREGAQ
                     KTDAQQTNKNLLLSDKARVDTKPQLEIYADDVKCAHGAAVGQIDPEELFYLEARGIGP
                     ELGRSLLTYGFAEEVIGKIKIESIRSQLDKFVLRQLVSTDYADFTEV"
     gene            1831..3057
                     /locus_tag="VFH96_02300"
     CDS             1831..3057
                     /locus_tag="VFH96_02300"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET6972823.1"
                     /translation="MANSWDIDRIRADFPVLQQTVNGKPLIYLDNSASSQVPQVVIDR
                     GSVYLEQEHSNIHRGVHYLSQKATTAYEGAREKVKRFINARDVRECIFVRGATEGINL
                     VMHSYGRKFIGAGDEIIISAMEHHANIVPWQMLCEEKGAKLRVIPMNDAGELLLDEYD
                     GLLNERTKLVAVMHVSNALGTINPIKDMIATAHKYGVPVLIDGAQAAPHMPIDMQDLD
                     ADFYVFSGHKMFAPTGSGVVYGKLELLEKMNPFQGGGDMIKTVTFEKTTYADPPNRFE
                     AGTPAIASQIGLGAAIDYLNSIGREAAAAYEMELLRYATAKVSAIEGVRIIGTAKNKA
                     SVLSFVIDDIHPHDIGTILDQEGIAVRAGHHCAQPVMQRFKVPATARASFAFYNTKEE
                     IDVLARTIERVIEIFS"
     gene            3057..3506
                     /locus_tag="VFH96_02305"
     CDS             3057..3506
                     /locus_tag="VFH96_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET6972824.1"
                     /translation="MSELSELYQQVILDHNKKPRNFRKLETANHTAEGFNPLCGDQLT
                     IYLNLEDDTVKEVGFEGSGCAISKAAASMMTQAVKGKSKEQAENLFQEFHSMVTGELN
                     EETEENSLGNLKIFAGVREFPVRVKCATLPWHTLHAALNKEAQVSTE"
BASE COUNT          912 a          970 c         1066 g          765 t
ORIGIN      
        1 ctgcgtctcg cgtacaacga aaagaagaaa tatctcggtg aggaagagct tgaccccctt
       61 gagttcaagg atcttttgaa agagcgtgcg aaggtcgtcg agatggatgc gagcttcatg
      121 acgcgctcgg tcaacgaaaa tttttcgggt ggcgaaaaga aacgcaacga gatcttgcag
      181 atggccgtgc tcgagccaaa gcttgccgtg ttggatgaga ctgattcagg tctcgacatc
      241 gacgcattgc gtattgtggc gaacggagtg aatcaactgt tcacgcccga gaatgcaatc
      301 atcgtcgtca cgcactacca gcgactcctg aactacatcg ttccacattt cgtgcacgtg
      361 ctggcggatg gtcgcatcgc gcgcgaaggc ggcaaggagc tcgcgctcga gctcgaagcg
      421 aagggatacg actggatcag gactgcagac aaaggagcgg gcgtgccggc gaactaggga
      481 tggttactga actcgttaaa tcagaaaata gttatcaagg ggcgtttcgc tcggcgcgtc
      541 ccaccgtggc gtggctcgag ctcgtgcgca acagcgcgat ggatcgtttc gagcaactgg
      601 gttttccgtc agtcaaagaa gaagactgga agtacacaaa ccttgcgacg cttgcgaagg
      661 aagagtttgt gccggtcacg actgggacca ctgacgtggg tgaagtcgag gggtatccgg
      721 aaacggccga gactcatctc gtagttgttg atggttttct gcgtgaagac ttatcaacaa
      781 agacaggact cggcgatgtg gtcgagatcg atttgttcaa tgcagtcgaa gacgcgcgct
      841 acaacaagat cgttcggaag tacctggcgc gcaacgccgg ctatcacaac aacgggctga
      901 ccgcgctgaa cacggctttc ctgcaatccg gtgtgtttct ctggattccg aagaacgtaa
      961 aactcgaacg accattgcag atcacgttcg tggctgatgc cgagcacggc gcgagttttc
     1021 cgcgactgct cgtggtcgct gaagaaaata gctcggcgac gctgatcgag agtttcgtgt
     1081 cgagtaatgg cggaaagtat ttcacgaatg cgactgcaga gatcgttctg aaagacggcg
     1141 cccagctcga gcactatcgc gtccagcgcg agagccacaa cgcgtatcac gtttcgacga
     1201 cctcggctga gcttggtcga gcgagccgtt actatacaac gtcgatcaat ctcggcgcgc
     1261 aattatcgcg acacgatgtt tcggtcgtga tggatcatga aggcgcggag accgctgttg
     1321 atggtctcta catggtcggc tccgatcagc acagtgatac gcattcggtg atcgatcaca
     1381 agcagccgca ttgcaccagt caccagttgt acaagggaat tctcgacggc aacggacgcg
     1441 cggttttcaa cggcaaggtg tttgttcgtg agggcgcgca gaagaccgac gcgcagcaga
     1501 cgaataagaa tcttttgctg tcggataaag cgcgcgtgga tacgaagccg cagttggaga
     1561 tttatgcgga tgatgtgaag tgtgcgcacg gcgcggcggt cggccagatc gatccggaag
     1621 aattgtttta tctcgaggcg cgcggcatcg gaccggagct tggccgcagc ttgttgacat
     1681 acggatttgc tgaagaagtg atcgggaaga tcaagattga gtcgatcagg tcgcagcttg
     1741 ataagtttgt gctgcgccag ttggtatcca cagattacgc agatttcaca gaggtctaaa
     1801 ttgagtagcg taataacatc agaggtcgct attgctaata gttgggatat tgatcgcatt
     1861 cgtgctgatt ttcccgtcct gcaacaaaca gtcaacggca aaccgcttat ctatctcgac
     1921 aactccgctt cctcgcaagt cccgcaagtc gtcatcgatc gcggcagcgt ctatctcgaa
     1981 caggaacact cgaacatcca tcgcggcgtt cattacctga gtcagaaagc cacgactgct
     2041 tacgaaggtg cgcgcgaaaa agtgaaacgc tttatcaacg cgcgtgacgt gcgtgaatgt
     2101 atcttcgtgc gcggcgccac cgagggcatc aacctcgtga tgcacagcta tggccgcaag
     2161 ttcatcggcg cgggcgacga gatcatcatc tccgcgatgg agcatcacgc caacatcgtc
     2221 ccgtggcaaa tgctttgcga agaaaaaggc gcgaagctgc gcgtgattcc gatgaacgac
     2281 gcaggcgagt tgctgctcga cgagtacgac ggcctgctca acgaacgcac gaagctcgtc
     2341 gcggtaatgc acgtctcaaa tgcgctcggc acgatcaatc cgatcaaaga catgatcgcc
     2401 accgcgcaca aatatggcgt ccccgtgctg atcgacggcg cgcaggccgc gccacacatg
     2461 ccgatcgaca tgcaggatct cgatgctgat ttttacgtct tctcgggtca taagatgttt
     2521 gcgccgacgg gcagcggcgt tgtttacggc aagctcgagc tgctcgaaaa gatgaacccg
     2581 tttcaaggcg gcggcgatat gatcaagacc gtcaccttcg aaaagacgac ttacgccgat
     2641 ccgcccaatc gttttgaagc cggcacaccc gcgatcgcgt cgcagatcgg tctgggtgct
     2701 gcgatcgatt acctgaacag catcggccgt gaagctgcgg ctgcatacga aatggagctg
     2761 ctgcgctacg ccaccgcaaa agtttccgcg atcgaaggcg tgcgtatcat cggcaccgca
     2821 aagaacaagg cgagtgtgtt gtcgtttgtg atcgacgaca ttcacccgca cgacatcggc
     2881 acgatcctgg accaggaagg cattgcggtt cgcgccggac atcactgcgc gcaacccgtg
     2941 atgcagcggt tcaaagtgcc ggcgactgcg cgcgcgtcgt ttgcttttta caacacgaag
     3001 gaagagatcg atgtgctcgc acgcacgatc gaacgtgtga ttgagatatt tagctaatgt
     3061 ctgaactgag tgaactctac cagcaggtaa tcctcgacca taacaagaag ccgcgaaact
     3121 tccgcaagct cgaaacggct aatcacaccg ctgaaggttt caatccactc tgcggcgatc
     3181 aactgactat ctatctgaac ctcgaagacg acacggtgaa ggaagtcggg tttgaaggat
     3241 cgggctgtgc gatctcgaaa gccgcggcca gtatgatgac gcaagccgtg aaagggaaga
     3301 gtaaagaaca agctgagaac ctttttcagg agtttcactc gatggttacg ggtgaactga
     3361 atgaagagac cgaggaaaac agtctcggca acctcaagat cttcgcgggc gtgcgcgagt
     3421 ttccggtgcg cgtcaaatgc gccaccctcc cctggcacac cctccacgcc gcactcaata
     3481 aagaagcgca agtctccacg gaataactaa cgccaaaggc gttcgctaat ttcagcccag
     3541 ggttggagcg ccagcgacaa ccctgggtat caatcaaata catgctaaac cctgaaaggg
     3601 ttaggcagtt ggcgaaccct tacagggtta ataccgagat taattggcga tcccagggtt
     3661 ctcgcaagct cgaaccctgg gctaaaattg gccaacgcct tcggcgtatt gcg
//
LOCUS       DASSFQ010000073         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2116807, whole genome shotgun sequence.
ACCESSION   DASSFQ010000073 DASSFQ010000000
VERSION     DASSFQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116807"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1156)
                     /locus_tag="VFH96_02310"
     CDS             complement(74..1156)
                     /locus_tag="VFH96_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6972825.1"
                     /translation="MIKKPAQRIRYLRTQDGVQLAWAEAGAGPLLIKAANWMTHLEYE
                     WESPVWRHWIRFFSEHFRYIRHDERGCGMTDWNVGDLSFARWIEDLEAVVEAVNPQEP
                     FALLGISQGAATCIAYAVKHPERVSRLVLYGAYARGTYRRGDPDKERLYRALIDMARL
                     GWGKDNPVFRQVFTSRFIPGATDEQIGWFNDLCRKTTSPEIAARLLETRATIDVMDLL
                     EHVQAPTLILHSRDDDVIPIAEGHILAAGIPGAQFVELDSKNHILLDSEPGWERFCAE
                     VLDFMNVKESAAEDPAFESLTAREREVLALITEGLGNAQIAERLSISEKTVRNHVTNV
                     FDKLGVWTRAQAMVFAHDRGFRTRSI"
     gene            1274..2005
                     /locus_tag="VFH96_02315"
     CDS             1274..2005
                     /locus_tag="VFH96_02315"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET6972826.1"
                     /translation="MLKRVLFFAYGTFSYLIFFGTFLYAIGFIGNFGVPRTLDGAATG
                     PLAVSLAIDVALLTLFAVQHSVMARKWFKEWWTRIVPKPIERSTYVLFSSLALILLFV
                     LWRPLGGVIWSVEDPAGRFVLRSLFAFGFGLVLLSTFLINHFDLFGLRQVWRHLLGHP
                     QGGLTFTTPGPYKLVRHPLYVGWLFAFWMTPVMTAAHLLFSIATTAYILLAIQFEERD
                     LVREHGETYETYRQSVPMLVPSLRK"
     gene            2195..3205
                     /locus_tag="VFH96_02320"
     CDS             2195..3205
                     /locus_tag="VFH96_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6972827.1"
                     /translation="MPARISVRDDESNEDFGIIGNNPKLRQVRTIIHTAAPSDASVLI
                     EGESGTGKELIATALHVCSERAARPFIRINCAAIPSTLIESELFGYRKGAFTGAHNDR
                     RGLIEATAGGTLLLDEIAEMPVYLQTKLLRVLQERTVRRLGDEREIPVDFRLIASTNR
                     DTSQMVKDGTLRTDLYFRVSTVKIVVPPLRERLDDLLLLSETFLLRLARKYKKPVRGI
                     APGTRSILMRYAWPGNVRELESVIEHAVLFARGEYLMPEDLPDQLSTSRKQNFLCVIP
                     PFLTKEDIEREAIAQTLERTGGNIKKTAQILNYNRPTLYRRLRQFGLRDPDSKPAKRA
                     TA"
BASE COUNT          742 a          955 c          974 g          773 t
ORIGIN      
        1 cgtttgcgcg tgcatcgcaa acggaagtaa gaaaaggaat aacagaatta cggcggatcg
       61 tttcataatt ctcctaaatt gatcgtgttc ggaagccgcg atcgtgcgcg aagaccatcg
      121 cctgtgcgcg cgtccagacg ccgagtttgt cgaagacgtt cgtcacgtga ttgcgcacgg
      181 tcttttcgct gatcgaaagg cgctctgcga tttgcgcgtt gcccaaacct tccgtaatca
      241 acgccagcac ttcacgctcg cgcgccgtta acgactcaaa cgcaggatct tcggcagcag
      301 attctttcac gttcatgaaa tcgagcacct cggcgcagaa gcgttcccac cccggctcac
      361 tatcgagaag aatatggttc ttcgaatcaa gctcgacgaa ctgtgcgccg ggaattcctg
      421 ccgcgagaat gtgaccttcc gcaatcggta ttacatcatc gtcacgcgaa tgcagtatca
      481 gtgtcggcgc ctgtacgtgt tcgagcagat ccatgacgtc gatcgtggcg cgcgtctcga
      541 gtagtctggc agcgatctcc ggcgaagtcg ttttgcgaca gagatcgttg aaccaaccaa
      601 tctgctcgtc ggtcgcaccg ggaatgaagc gcgaagtgaa aacttgccgg aacacgggat
      661 tgtccttgcc ccagccgagt cgggccatgt cgatcagcgc gcgatacaaa cgctccttgt
      721 ccggatcgcc gcggcgatac gttccacgcg catacgcgcc gtaaagcacg agccgcgaca
      781 cgcgttccgg atgtttcact gcatacgcga tgcacgtcgc cgcaccctgc gaaataccga
      841 gcagcgcgaa cggttcctgc gggttaactg cctcgacaac tgcttcgaga tcctcgatcc
      901 agcgcgcgaa tgacaggtcg ccgacgttcc agtcagtcat tccacaaccg cgctcatcgt
      961 ggcgaatgta tcggaaatgt tcgctgaaga agcggatcca gtgacgccaa accgggcttt
     1021 cccattcgta ttcgaggtga gtcatccagt ttgcggcttt gatcagcagc gggccggcgc
     1081 ctgcttccgc ccaggcgagt tgcactccgt cctgagtgcg cagatatcga attcgctgtg
     1141 ccggcttctt gatcatgctg agacaaaagt cctatgcgga agcccggaag tatagccaaa
     1201 ccgggactaa actcccatag cctgccggcg cgaatcgcgc gatcctgcgc ggcacgaaag
     1261 aggaggcaat accatgctga agcgtgtttt atttttcgct tacgggactt ttagttacct
     1321 gattttcttt ggaacgtttt tgtacgcgat tggcttcatc ggcaacttcg gagtgccgcg
     1381 cacgctcgac ggcgccgcga ccgggccgct cgccgtcagt ctcgcgattg acgtcgcact
     1441 gctcacgctt ttcgccgttc agcacagcgt gatggcgcgc aagtggttca aagaatggtg
     1501 gacgcgaatc gtgccgaagc cgatcgagcg ctcgacttac gtgctcttct cgagtctcgc
     1561 gttgattttg ttgtttgtgt tgtggcgacc actcggcggc gtgatctggt cggttgaaga
     1621 tccggctgga cgattcgtgt tgcgcagtct atttgccttt ggctttggac tggttctgtt
     1681 gtctaccttc ctgatcaacc atttcgattt gttcgggttg cgtcaggtgt ggcgccactt
     1741 gctgggtcac ccgcaaggcg gtttgacgtt tacgactccc ggtccataca agctcgtgcg
     1801 acacccactc tacgtcggct ggctgttcgc tttttggatg actccagtaa tgacagccgc
     1861 gcatttgctg ttttcgatcg caacaacagc ttacatcctg ctcgcgatcc aattcgaaga
     1921 acgcgatctc gttcgcgaac atggcgaaac ttacgaaact tatcgtcaat cagtgccaat
     1981 gcttgtccca tcactgcgca aataagaagt aggccgcgga ttttcgcgga tgaacgcaga
     2041 tctgatccgc gttcatccgc ggctaataaa ttctcttgac caaaatgccg caactttagc
     2101 atcgattcgt ctaacttaaa gcgcatcatc ccgcccttca ctcaaggtct cgactctcac
     2161 gcccagaaga ctaaggaagg aagggtgtgc ccacatgcct gcccgtatat ccgtacgtga
     2221 tgatgagagt aacgaagact tcggaatcat cggcaacaat ccgaaactgc gccaggttcg
     2281 cacgatcatt cacaccgccg cgccgtctga tgcttcggtt ctgatcgaag gcgagtcagg
     2341 cacgggtaaa gagttgatcg cgacggcgtt acacgtttgc agcgaacgag ccgcgcgacc
     2401 gttcattcgg attaactgtg ccgcgatccc gagcactttg atcgagtcgg agctgtttgg
     2461 ttatcgcaaa ggcgcattta ccggcgcgca caacgatcgc cgtgggctga tcgaagcgac
     2521 tgccggcggt acgctgctgc tggacgagat cgcggagatg cctgtttatt tgcagacgaa
     2581 gttgttgcgc gtcttgcagg agcgaacagt acgtcgactc ggcgacgagc gcgagatccc
     2641 cgtggatttt cgtctgattg cgtcaaccaa tcgcgacacg agtcaaatgg taaaggacgg
     2701 aacactgcgt acggacctgt attttcgcgt gagcaccgtg aagatcgtag tgccgcctct
     2761 gcgagagcga cttgacgatc tgcttttact aagcgagacg tttttgctgc gacttgcgag
     2821 gaagtacaag aagccggtgc gcggaatcgc gccggggacg cggtcgatac tgatgcgata
     2881 tgcgtggccg gggaatgtgc gtgagctgga aagtgtgatc gagcacgcgg ttctgtttgc
     2941 gcgcggggaa tatttgatgc cggaagatct gccggatcag ttgagcacgt cacgtaagca
     3001 gaattttctg tgcgtgatac cgccgttttt gacgaaggaa gacatcgagc gcgaggcgat
     3061 cgcccaaacg ctcgagcgca cgggcggcaa catcaaaaag accgcgcaga tcctcaacta
     3121 caaccggcca accctttatc gacgcctacg ccagttcggg ttacgcgatc ctgattccaa
     3181 gcccgcgaag cgcgcgacag cataaaggtt gataagcccc tgaagcgggc tacagcataa
     3241 aggttgataa gtccgtgaag cgcgcgacag catcaaggtt gataagcccg cgaagcgggc
     3301 gaaagcataa agccttctat gaagccgggt cgtgtcaaga gatgagttcg gggccgattg
     3361 gttcaccctt ctattcgagg tatccacgcc ggtgagccgg agctctgaac tttgtggtct
     3421 cactcaacgg tgcgacgagg ctga
//
LOCUS       DASSFQ010000074         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_29316, whole genome shotgun sequence.
ACCESSION   DASSFQ010000074 DASSFQ010000000
VERSION     DASSFQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29316"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1277)
                     /locus_tag="VFH96_02325"
     CDS             complement(258..1277)
                     /locus_tag="VFH96_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972828.1"
                     /translation="MGIEFKNVNKTFGGLVVVDDLSLKIDDGEFVVLLGPSGCGKTTT
                     LRMLAGLESISSGEILINGELVNDVPTQHRDLAMVFQSYALYPHMTIAENIGYPLRVR
                     KIEQTERTKRVKRVAEMLEIESLLERKPRQLSGGERQRVALARAIVREPRAYLMDEPL
                     SNLDARLRVQMRGELKHLQHQLGTTTIYVTHDQAEAMTLASRVAVMKKGRLQQFDTPM
                     NIYNYPANRFVAEFVGSPSMNFIEGSPLTIGIRPEHIQVLSQPQDGAIPAKVFVTEQM
                     GNETFVFLNVGTNRLIARAPADFRAEEESRVWITINKDKAHFFDPVSGERRATDNTDW
                     IRIGV"
     gene            complement(1278..>2129)
                     /locus_tag="VFH96_02330"
     CDS             complement(1278..>2129)
                     /locus_tag="VFH96_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6972829.1"
                     /translation="QSANKLAHSKATTMLRYVALLAALLFALAPAYWMLTISLKTEVE
                     QFAVPPPWFVFTPTLTHYQDAFVTRGFGNYLLTSAIVAVTSTACALVIGTLAAYALAR
                     FQLPWRLNRRLSLWILSTRMFPAIVTAVPLFLMMRDVQLLNTRLSLIIVYTGFNLPFV
                     VWMMRGFFADLPRDLEEAALVDGDSRIGALVRVVLPLVSPGLAATAVFCLIVSWNEFL
                     FALVLTQTDASMTLPVGIAGSVTQFEIKWGVMSAAATVAVVPILVFALSLQKYLVRGL
                     SMGAVKG"
BASE COUNT          507 a          569 c          589 g          464 t
ORIGIN      
        1 ctttttcatg cttgaaagtc ctttcttgct ttgcttttct cgactgaatg gaacgggtcc
       61 tgcagagagc gggataagca ggatcaggtg atcccaaatt gttttccgca cgtgtagctg
      121 ctgaggcacg taagcacaca gaaggaagtg ctactcacag ctgggccctg tctctcaagg
      181 agtgccgggg acattatgct gctggtagga taaagtcaag gaatcgagaa caactattat
      241 taatcgtatt ttggaaatta cacaccaatc cggatccaat ccgtgttatc ggtggcccgc
      301 ctttctcccg agacaggatc aaagaaatgg gctttgtctt tgttgatggt gatccagacg
      361 cgactttcct cctcagcgcg aaagtcggcc ggcgcgcgag caattaaccg gttagtaccg
      421 acgttaagaa aaacgaaagt ttcatttccc atttgctccg tgacgaaaac ctttgcgggg
      481 attgcgccgt cctgtggctg tgataacact tgaatgtgtt ccggtcgaat cccaattgtt
      541 aacggtgatc cctcgatgaa gttcatgctc ggactgccga caaactcggc gacaaatcga
      601 ttcgccgggt agttgtagat attcatcggc gtatcgaatt gttgtagccg gcctttcttc
      661 attaccgcta cgcgcgacgc cagcgtcatt gcttcggcct gatcgtgagt gacgtagatc
      721 gtggtggtgc cgagctgatg ctgaaggtgc tttaactcgc cacgcatctg aacgcgcaga
      781 cgcgcatcga gatttgaaag cggttcgtcc atcagataag cgcgcggctc acgcacgatt
      841 gcacgcgcca gcgcgacgcg ttgtcgttcg ccgcccgata gttgacgcgg tttgcgttcg
      901 agcagcgatt cgatttcgag catttccgcg acgcgtttta ctcgttttgt tcgttcggtt
      961 tgttcgatct tgcgaacgcg caacgggtaa ccgatgttct cggcgatcgt catgtgcgga
     1021 taaagcgcgt aactctggaa caccatcgcg aggtcgcggt gctgtgtcgg cacgtcgtta
     1081 acgagttcgc cattgattag aatttcacct gagctgatcg attcgagacc ggcgagcatg
     1141 cgcagagtcg tagttttgcc gcagccggat ggtccaagga gcacgacaaa ttcaccgtcg
     1201 tcgatcttca gcgacagatc gtcgacgacg acaaggccgc cgaacgtctt attcacattc
     1261 ttaaactcaa tacccatcta tccttttact gcccccatcg acaatccgcg cacgaggtat
     1321 ttctgcaacg agagtgcgaa cacaagaatc ggcacgaccg ccacggtagc cgcagcgctc
     1381 atcacgcccc acttgatctc gaactgagtg acgctgccgg caattcccac tggtaaagtc
     1441 atcgacgcat cagtctgagt caacacgagt gcaaacagga attcattcca cgaaacaatc
     1501 aagcagaaca cggccgttgc ggccagtccc ggcgagacca gcggcagcac cacccgcacg
     1561 agcgcgccga tgcgcgagtc gccatctacg agtgccgctt cttcgaggtc gcgcgggaga
     1621 tcggcaaaga aaccacgcat catccagact acgaacggca gattgaagcc ggtgtaaacg
     1681 atgatcagcg atagacgtgt gttgagcagt tgcacgtcgc gcatcatgag aaagagcggt
     1741 acggccgtaa cgatcgcggg gaacatgcgc gtcgagagaa tccacagcga gagacggcga
     1801 ttgaggcgcc atggcagttg aaatcgcgcc agtgcgtaag cggcgagtgt gccgatgacg
     1861 agtgcgcacg ccgtcgaagt aacggcgaca atcgcgctgg tgagtaaata attgccgaaa
     1921 ccgcgtgtga caaaagcatc ctggtaatga gtgagtgtgg gagtaaaaac aaaccacggt
     1981 ggcggaacag caaactgttc cacctccgtc ttcagagaaa tcgtcaacat ccagtaagcc
     2041 ggcgcgagcg caaacaacag cgcagcaagc aacgcgacgt accgtaacat ggtcgtcgcc
     2101 ttggagtgcg ccaacttgtt ggcgctttg
//
LOCUS       DASSFQ010000075         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_30263, whole genome shotgun sequence.
ACCESSION   DASSFQ010000075 DASSFQ010000000
VERSION     DASSFQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30263"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFH96_02335"
     CDS             <1..885
                     /locus_tag="VFH96_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972830.1"
                     /translation="SVELTVAPNAPFGDYTLRLQSNSGEIAYVPGAITIDPGVTLPVA
                     NPVDDSRFFIAQQYADLIGHDPDQTTVDRLLAQINACNSRSDCVRTRRVEISTNLLVE
                     NELPATGVFVYGLYSAAFARLPRFMELETDRAVTMNNKGEVEAVRLALASAFVERAEF
                     KSKFPATMKPSEFVDSILATLAQTSGVDFISERSLLINLLDDAANGRAMVLTRIASDQ
                     RVVDANYNQALVLYQYFTYLRRSPDEAGYNAWVNTLKNKPLRDPDAARSLVCNFLHSA
                     EYQNRFGMVATRHSRECN"
     gene            916..1908
                     /locus_tag="VFH96_02340"
     CDS             916..1908
                     /locus_tag="VFH96_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6972831.1"
                     /translation="MKKSLLAYLICPTCEGTLDLSVAKTEAEEIMEGELRCLSCSTTF
                     PIRSGIPRFANLDHVESDKLATATNFGWQWQHFTQEDERYAEQFLGWIAPVTPEFFRD
                     KVVLEGGCGKGRHTQLAARWGAREIIGIDLSGAVETAFAATRSLPNAHIVQADIYHLP
                     LARTFDYAFSVGVLHHLPDPRGGFLSLASKVKPGGHVSAWIYGAENNEWITRWVNPVR
                     EKITSRIDRRALLQLSKLPAACMYLATKLVYGPLNRGGSSLAPHLFYNDYLNAISGFG
                     WREQHTIVFDHLTAPTAFYISRDEFETWWRDINARDVEIGWHNKNSWRGRGMIQ"
     gene            1905..2738
                     /locus_tag="VFH96_02345"
     CDS             1905..2738
                     /locus_tag="VFH96_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972832.1"
                     /translation="MNSSVMQSTGHRNVWLPLWQLPSRAELIFSFAKRELLARYKGSA
                     LGIAWAVLTPVVMIAIFTFIFAGIFGARFGANDSHWDYALYLFCGLLPWTMFQESVQL
                     SANTIVAHSNLVKRVVFPLEALPAAQVFAALGTQLFGTMALLIGTIVIRQRLELTGLW
                     LPILLIPQLLFALGAGWLVASLGVFLRDITQGVTLLLMAWMYLTPIIYPESIIPEHFR
                     TFINFNPFTSLIRSYRRIFLDGSVPDWRGLLYFTIFAVIIFIFGYWWFARTRKSFADV
                     V"
     gene            2841..>3559
                     /locus_tag="VFH96_02350"
     CDS             2841..>3559
                     /locus_tag="VFH96_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6972833.1"
                     /translation="MNKARFIGGVIVLLFVATTGAEAQDWTQWRGPGRDGLVVSRYAP
                     ATWPASLKQTWHVEIGEGYSSPVVAGGKVFVHGRRDPEEIVAAFNLTDGKLLWQQKYQ
                     AEYKKNQYAVEMAKGPNATPLIAGNRLFTFGVSAILNAWDTATGKLLWTRDFSKSIDT
                     SKLFCGTAASPLIVNGRLIVQVGSDIHGGQVLAVNPATGATEWEWRGLGPGYASPVVI
                     ELGGSQQIVAMTEGSIVSLDG"
BASE COUNT          719 a          981 c         1003 g          856 t
ORIGIN      
        1 agcgttgagt tgacggttgc gccgaacgcg ccgtttggtg attacacgtt gcgtctccaa
       61 tcaaactccg gcgagatcgc gtacgtgccg ggcgcgatca cgatcgatcc gggagtgacg
      121 ttgccggtcg cgaatcctgt agatgattct cgtttcttta tcgctcaaca gtacgcagat
      181 ctgatcggac acgatcctga tcaaacgact gtcgatcgtt tgctggcgca gatcaacgcg
      241 tgcaattctc gcagcgattg tgtgcgcacg cggcgagtgg agatctcgac caatctgttg
      301 gttgaaaatg aactgccggc gaccggcgta tttgtttacg gtttgtattc tgctgccttt
      361 gccaggctgc cgcgattcat ggagttggag actgatcgcg ctgtgacgat gaataacaag
      421 ggcgaagtgg aagccgttcg actagcgctg gcgagcgcat ttgtggaacg cgccgagttc
      481 aaaagtaagt ttcctgccac catgaaaccg tctgagtttg tcgactcaat tctggcgacg
      541 ttggcgcaga cttcaggcgt cgactttatc tcggagcgga gtctgttgat taacctgctt
      601 gatgatgcag ccaacggacg cgcgatggtt ttgacgcgca tcgcttccga tcaacgcgtg
      661 gtggacgcga attacaacca ggcgctggtg ctctatcagt acttcactta cctgcgacgc
      721 agtccggacg aagcgggtta caacgcttgg gtcaacacct tgaagaacaa gccacttcgc
      781 gacccggacg cggcccgatc gctcgtctgt aatttcctcc actccgctga gtatcaaaac
      841 cgcttcggca tggtcgccac gcgccacagc cgcgaatgta attagatccg tggctaaatg
      901 ttatagtggc ttcgcttgaa gaagagtcta ctcgcgtatc tcatctgccc aacgtgtgaa
      961 ggaactctgg atctctcggt tgcaaagacc gaagctgaag agatcatgga gggtgagctg
     1021 cgttgtttaa gctgctcgac tacgtttccg attcgctccg ggattccgcg tttcgcgaat
     1081 ctcgatcacg tcgagagcga caaactggcg accgctacaa acttcggttg gcaatggcaa
     1141 catttcactc aggaagacga acgatacgca gaacagtttc tcggttggat tgcgcccgtt
     1201 acaccggagt tctttcgcga caaggtcgtg ctggaaggcg gctgcggtaa aggccgccac
     1261 acgcaactcg cggcccgctg gggcgcacgc gagatcatcg gtatcgattt gagtggcgcg
     1321 gttgaaaccg cgttcgcggc gactcgttca ctacctaacg cacacatcgt tcaggccgat
     1381 atctaccact tgccgctggc ccgaacgttt gattacgcct tttcagtcgg cgtgttgcac
     1441 caccttccgg atcctcgtgg cggtttcctg tcgctggcgt cgaaagtaaa gccgggcgga
     1501 cacgtttcag cgtggattta cggtgcggaa aataacgagt ggatcacgcg ctgggtgaat
     1561 ccagtgcgtg agaagatcac gtcgcgcatc gatcggcgcg cgcttttgca gttgtctaaa
     1621 ctgccggcgg cttgtatgta tctcgccacc aagctcgttt atggaccact caaccgcggt
     1681 ggctcatctc tggcgccgca tcttttttac aacgactacc tcaacgcgat ttctgggttt
     1741 ggttggcgcg agcagcacac aattgttttc gatcatctca ctgcgccgac cgcgttctac
     1801 atttcgcgtg atgaattcga aacgtggtgg cgcgacatta acgcgcgcga cgttgagatc
     1861 ggatggcaca ataagaacag ttggcgcggc aggggaatga ttcagtgaat agctcagtga
     1921 tgcaatccac cggtcatcgc aacgtttggc ttccgctctg gcaactcccg tcgagagcgg
     1981 agctaatctt ttcgtttgcg aagcgcgagt tgctcgctcg ttacaaaggc tcggcgctcg
     2041 gcatcgcgtg ggcggtgctc acccccgtcg tgatgatcgc gatcttcacg ttcatcttcg
     2101 ccggcatctt cggagcacgt tttggcgcca atgattcgca ctgggactac gcgctttatc
     2161 tcttttgcgg cttgttgccc tggacgatgt ttcaggaatc cgtgcaacta tcggccaaca
     2221 cgatcgtggc ccattcaaat ctcgtcaaaa gagtcgtctt tccgctcgag gcgttgccgg
     2281 cggcgcaggt attcgccgca ctcggcaccc aactcttcgg gacgatggct ttactgattg
     2341 gaaccatagt catccgccag cgactcgagc taaccggatt gtggctgccg atcctgctta
     2401 tcccgcaact gctgtttgca ctcggcgccg gctggctcgt tgcctcgctc ggtgtattct
     2461 tgcgtgacat cacgcagggg gtcacgttgt tgttgatggc gtggatgtac ctgacgccga
     2521 ttatctatcc ggaatcgatc atcccggagc atttccgcac cttcatcaac ttcaatccct
     2581 tcacctcact aatccgcagc tatcgtcgca tctttctcga cggctccgtc cccgactggc
     2641 gcggtctcct ctatttcacc atcttcgccg tcataatctt catcttcggc tactggtggt
     2701 tcgcccgcac ccgcaagagc ttcgccgacg tggtttagcc acaaaaaggc acaagatgca
     2761 caagcaggaa aacctattta attgtgcttt ttgtgccttt ttgtggcatg atctttcccc
     2821 tgtctttgga agaggtattc atgaataagg cgagattcat tggcggcgta atcgtcctac
     2881 ttttcgtggc aacaaccggt gcggaggcgc aggattggac ccagtggcgc gggccggggc
     2941 gcgatggcct cgtggtcagc aggtatgcgc ccgcgacgtg gccggcgtca ttgaagcaga
     3001 cgtggcacgt cgaaatcggt gaaggatatt cgtcgcccgt agtggctggt ggcaaggttt
     3061 ttgtccacgg gcgtcgcgat ccggaagaga tcgtggctgc cttcaatctc accgatggca
     3121 agttgctatg gcagcaaaag tatcaggccg aatataagaa gaaccaatac gccgtcgaaa
     3181 tggcgaaagg cccgaacgcg actccgctca tcgccggtaa tcgactgttc acctttggcg
     3241 tgtccgcaat tctcaacgcc tgggatactg ccaccggcaa gctgctttgg accagagact
     3301 tttcaaagtc gatcgacact tcaaaattgt tttgtggcac cgcagcgtca ccattgatcg
     3361 tgaatggtcg tctcatcgtc caggtcggca gcgatattca tggtggacaa gtgctcgcgg
     3421 taaatcccgc cacgggcgca accgagtggg agtggcgcgg acttggacca ggctacgcgt
     3481 cgccagtggt aatcgagctc ggcggtagtc agcaaattgt tgctatgacc gaaggctcga
     3541 tcgtgagtct cgacggaaa
//
LOCUS       DASSFQ010000076         8267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_928470, whole genome shotgun sequence.
ACCESSION   DASSFQ010000076 DASSFQ010000000
VERSION     DASSFQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928470"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..2656)
                     /locus_tag="VFH96_02355"
     CDS             complement(167..2656)
                     /locus_tag="VFH96_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6972834.1"
                     /translation="MPREITDRGRVVEAIQNLALTAELFEEQNGKQKYGTDLEVIVQG
                     RDYANGKRVGPYVRLLTYDSGEEIMRQGEWGGNTFYITVEGTLDVYVRDSPGNPVNIA
                     QKKIGQLEPGTCFGEMALLAGIARNATVSVPANQTALVLEVTRPALRLLRKLSTFGTI
                     LDTTYRTHGIDRLLEDLRLVTGKQLSEPLVAELHRTARYMVYSKNHALCKEGETIDCL
                     ILIKNGWIRRSHGVFFDAASPEVVLGMDEGIGVDFLGGGNCLGIEGVSKPEVWKYKAS
                     VMARTEVLEIPLDQFSTDPALRKQLVDTFAELCTAGSAIPSTIEDLPDLNALKAAEEE
                     ITTGIIDGTNVLVMDMDLCIRCGNCSLACHKMHGQSRLLRRGIQIERPTKIAKKRLQH
                     ALVPQVCMHCADPECLTGCPTGSIFRAPLGHIDIDPQTCIGCFDCATQCPYDAITMVP
                     RAAPPVSNGFASKLRKAFSLRNDRLVTAEPSDDPVAIKCNLCEDTPLNPPGARRKAYS
                     CEENCPTGALVRVDPIEYFSEIGPAQGFVFRDQTHAVGRNIHKSDPLARAWHIGGAVV
                     TILTAAVLLWALGKYGFDTVLAGTWLTLRWLTGIVGFAGVAVVMSYPLRKQVYRRRAG
                     ALRYWMLVHVYVGAIAGVVLLFHSGPRTGGLLTSLLYVAFDLTLLSGIVGIVSYLVMP
                     RIMTRIEGEPLLVEDLEARRKELRSDTRTILEKSDGWLKEEIRDRVYPRFLSWGFLWR
                     QVWRREELKALLAQARQEFKDRTTRLTTDEEREFLIDAVETAVMLRRVDALLTLHWLL
                     RFWIPMHIAATSVMLALMLVHIAQVVFFRVN"
     gene            2794..3939
                     /locus_tag="VFH96_02360"
     CDS             2794..3939
                     /locus_tag="VFH96_02360"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HET6972835.1"
                     /translation="MLPENLYALPLEQQIGQFLFIGLPGTEFDAEARALVEEVQPGGV
                     IIFGRNVAGPEQLRSLLDAVREVVPTPPLFGIDQEGGLVDRLRRIFTPMPSARTIRQH
                     GDLAAARALGRVTGEVLRMLGFNMNFAPVMSIMTEDRDLLSNGLYSRSFGRSPGEVLG
                     YTTVYMRGLQGTGMLGCLKHFPGIGAGEVDSHEEMPVVRLSHDDLIAQDLAPYIELFQ
                     RRDDRVRCVMVSHGGFPNIDIKGEMTGGLLEPASLNYNIVTTLLRQELGYKHLVVTDD
                     LEMGAIARHCEIEDAVVRATLAGEDMLLICASPDKIRRGYHGLLEAASSGKITEERIK
                     QTLERIARTKTIMEPPVPLDTARFQTLADEILKLNSKLNYTYGGTVK"
     gene            3936..5510
                     /locus_tag="VFH96_02365"
     CDS             3936..5510
                     /locus_tag="VFH96_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6972836.1"
                     /translation="MSKGKLLLLLVLTLLLIIPPACRRRSNAFVIALGDNVRTIDPIG
                     APAVEAASERVRTLMFNTLVKKDDKFDYVGELASDIKRSDDGLSYTFTLRDGVKFHNG
                     QALSSADVKYTLDLVFSSNFGKSASFYKGQGAEKRSYIKSVEAPDARTVVVTLTEPWV
                     GLLSNLVPIPMIPKDSYESQKTHPLGTGPFKFVHYDNAQQVCDVEAFPDYWEGAPKLQ
                     SVRVRVISDMNALQAELQSGRVDIAPLPTSLSPDAVKRLEGDPNLQVKVFNGSNVNLL
                     TINTASPPLNNVKVRQAIAYAIDRESLIKNLLLGLGKIANSIIPEESWAYSPGQTYSY
                     DPARAKQLLDEAGFRDPDGDGPRMRFDKPIVFKLSGSSIAGRQYAGVIQNSLKEVGIP
                     VEIETAEQNTFLDELKRGNFQMSYSQWVGGNQDPIFYKDLFASSEIPTETRSARNRSR
                     YSNKDLDALLNEALNTYDHQKARELYVRIQEIVSRDVPVFPLWYQSNIVIARKDVQNI
                     QVNASGDWGFVRNLSR"
     gene            complement(5593..6588)
                     /gene="tsaD"
                     /locus_tag="VFH96_02370"
     CDS             complement(5593..6588)
                     /gene="tsaD"
                     /locus_tag="VFH96_02370"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET6972837.1"
                     /translation="MSQDVILGIESSCDDTAAAVVDRDGNVLSSIIASQTHIHSHWGG
                     VYPEFASRAHALVVVPTIERALKEANVEGPDLKAIGVTRGPGLIGSLIVGVNTAAGLG
                     LGWNVPVLGINHLRGHLRSADLVEKRVELPAIVLLVSGGHTFLALMESLTSIQLLGST
                     RDDSVGEAYDKVARLLGLGYPGGPAVDKLAKTGRDSIPFPRPMLSEGFEFSFSGLKSA
                     VGRFLEKNPDVDHADVAASFVSSCMEILLTKCRRALEEYPAECLVVVGGVAASPQLRE
                     GAAALCDRMGVKLCLPPLKWSTDNAAMIGLAAWDYLNAGVTPPLSATASLKITEF"
     gene            complement(6585..7181)
                     /locus_tag="VFH96_02375"
     CDS             complement(6585..7181)
                     /locus_tag="VFH96_02375"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET6972838.1"
                     /translation="MKDGLINEIVECLGNGGVVLIPTDTVYGLAVSPEFDESIDRLFA
                     LKRRPRNVNLPIMVASDAELEALGFEVSESARRLLRSPLIPGSLTLAMGFSSDYRPAW
                     LAGRDEAAVRIPNDKRLLSVLRKTGPLLVTSANAHSAETPDNVADILAQLDGAPDLSI
                     DGGTLRTTASTLVNCRIDPPAIERIGVVPESEVLRYLA"
     gene            complement(7204..7884)
                     /gene="tsaB"
                     /locus_tag="VFH96_02380"
     CDS             complement(7204..7884)
                     /gene="tsaB"
                     /locus_tag="VFH96_02380"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET6972839.1"
                     /translation="MATGARCIEEPGMSLLLAIESSSNVYRVVLGRDGESIFDSAVND
                     CDQSLRTLSALLECGLESIGATASEIKGVAINVGPGSLTFVRAGISFVNALAFSLNVN
                     IYSFNWFEIIAEQTRETTELPVVCAVPASNDNAYVGLIRGESVEIMRFGRVSEALAKV
                     SDGLGDVAVAGRIRHRLAALLGEARVVDTGIENPDARTLLQLASRNGARSTRQVEALN
                     DQSDVFFE"
     gene            complement(7845..>8267)
                     /gene="tsaE"
                     /locus_tag="VFH96_02385"
     CDS             complement(7845..>8267)
                     /gene="tsaE"
                     /locus_tag="VFH96_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET6972840.1"
                     /translation="VAAALAPLLRPGDAMMLKGPLASGKTAFVQELVAALGSTAEVTS
                     PTFTIAQFYPIPTGTFLHIDAYRLSSVAEFRDLALEDFVAESITAVEWGDIVEPEFPT
                     ALSIDFEFVENEDNSRRLTFSATAEQWQPVLVALKNQV"
BASE COUNT         1913 a         2444 c         2195 g         1715 t
ORIGIN      
        1 ttgagtagca attcacactg cggcagccga ccaatcagca atccctcact gataatcgtc
       61 gccggatcga ccatcagatc gtcacgaatg atcacgtatt ggttcgtcgt catatttttt
      121 gccacagatt acacggataa cacagatcag cagtgtaaat ccgtggctaa ttcactctga
      181 aaaacacgac ttgcgcgatg tggaccagca tcagtgcgag catgaccgac gtggcggcaa
      241 tgtgcatcgg tatccagaag cgtaataacc agtgaagcgt caacaaggca tcaacgcgtc
      301 gcagcatgac cgcggtctcg accgcgtcaa tcaaaaactc tcgttcttca tccgtcgtga
      361 gtcgtgtcgt ccgatctttg aactcctgtc gcgcctgggc caaaagcgct ttcaactcct
      421 cacgccgcca gacctgtcgc cacaaaaatc cccaggacag gaaacgcggg taaactcgat
      481 ccctgatctc ctcctttaac catccatcgc tcttttcgag aatcgttcgc gtgtcgctcc
      541 gcaactcttt ccggcgggct tcgagatcct caaccagcaa cggctcgcct tcgatacgcg
      601 tcatgattcg cggcatcacg aggtaagaaa cgatgccgac gatccccgac agtaacgtca
      661 gatcgaatgc gacataaagc aggctcgtga gcaaaccacc cgtgcgcggc ccggagtgaa
      721 acaacagcac cacacccgct atcgcgccaa cgtaaacgtg gaccagcatc cagtaacgca
      781 aagcgcccgc tcgtctgcgg taaacctgct tccgaagcgg atacgacatc acgaccgcca
      841 caccggcgaa cccaacaatt ccggtgagcc agcgcagtgt tagccacgtg ccggcaagca
      901 cggtatcaaa tccatatttg cctaacgccc ataacaaaac cgcagcagtc agaatcgtca
      961 caacagctcc gccgatgtgc cacgcgcgcg cgagtggatc gctcttgtga atgtttcgac
     1021 caaccgcgtg agtctgatcg cgaaacacaa atccctgcgc cggaccaatc tcactgaaat
     1081 actcgatcgg atcgacacgc acgagcgcgc cagtcggaca attctcttca cacgaatacg
     1141 ccttgcgccg cgcacccggc ggattgagcg gcgtgtcttc gcaaagattg cacttgatcg
     1201 cgaccggatc gtccgacggc tccgctgtca ccagccgatc attccgcagg ctgaaagctt
     1261 ttctgagttt cgaagcgaaa ccgttcgaca ccggcggcgc agcgcgcggc accatcgtga
     1321 tcgcgtcgta cggacactgc gtcgcgcaat cgaagcagcc gatacacgtt tgcggatcga
     1381 tgtcgatatg gcccaacgga gcgcgaaaga tcgaacccgt cggacaacca gtcaaacatt
     1441 ccggatccgc gcagtgcata cagacttgcg gcacgagggc gtgttgtaaa cgcttcttgg
     1501 caatctttgt gggacgctct atttgaattc cccggcgtaa taagcgcgac tgcccgtgca
     1561 tcttgtgaca cgcgagcgag cagttgccac agcggatgca aaggtccatg tccatcacga
     1621 gcacgttagt gccgtcgatg atgccggtcg taatctcctc ttccgctgct ttcaacgcgt
     1681 tgaggtcggg aagatcttcg atcgtggatg gtattgcgct gcctgcagtg caaagttcgg
     1741 cgaacgtgtc tacgagttgt ttgcgaagcg cgggatcggt cgagaactga tcgagcggta
     1801 tctcgagcac ttcggtgcgc gccatcacgc tggctttgta tttccaaact tcaggtttac
     1861 tcacgccctc gatgccgaga cagtttccgc cgccaaggaa atcgaccccg atgccttcat
     1921 ccattcccaa cacgacttcc ggtgaggccg catcaaaaaa gacgccgtgt gagcgacgaa
     1981 tccagccgtt ctttataaga atgagacaat cgatcgtttc gccttctttg cagagagcgt
     2041 gattcttgct gtagaccatg taacgcgcgg tgcggtgcag ctcagcgacg agtggttcgc
     2101 tgagctgttt gccagtgacg agccggagat cttcgaggag acgatcgatc ccgtgggtgc
     2161 gatatgtcgt gtcgagaatg gtgccgaagg ttgagagctt gcgcagcagt cgcaaagcgg
     2221 gacgagtgac ttcgaggacg agggcggttt gattcgcggg caccgagact gtcgcattgc
     2281 gtgcgatacc ggcgagcaag gccatctcgc cgaaacacgt acccggttcg agttgaccga
     2341 tcttcttctg cgcaatattg acgggattgc cgggtgaatc gcgtacgtaa acgtcgagcg
     2401 taccttcgac agtgatgtaa aaggtgtttc cgccccactc gccctggcgc ataatctctt
     2461 cacccgaatc gtaggtgagc aggcggacgt aaggcccgac gcgctttccg ttcgcgtaat
     2521 cgcgaccctg aactatgact tcgagatcgg tgccatactt ctgcttgccg ttttgttctt
     2581 cgaagagttc agcagtaaga gcgagattct ggatggcttc aacaacgcga ccccggtcgg
     2641 taatttcgcg tggcatgaat cgtttataca tcatttagcc gcaaaaaggc acaaaaagga
     2701 caaaagcgaa attggagttc aaactcgaaa gtaccaaata agcttttgtg ctttttgtgc
     2761 ctttttgcag ctaaatgata aactcgcgaa cagatgctgc cagaaaatct ttacgcgctt
     2821 ccccttgaac aacaaatcgg acaattccta ttcatcggtc ttccgggaac cgaattcgac
     2881 gccgaagcac gcgccttagt cgaagaagtt cagcccggcg gcgtgatcat cttcggtcgc
     2941 aacgtcgctg gtccagaaca actccgctcg ctgctcgatg cggttcgcga agtcgttcca
     3001 acaccaccct tgttcggcat cgatcaggaa ggcggtctcg tcgatcgact gcgacgcatc
     3061 ttcacgccga tgccatccgc gcgaacgatt cgccagcacg gcgatctcgc ggcggcgcgc
     3121 gcgctcggta gagtcaccgg cgaagtgctg cgcatgctcg gcttcaacat gaacttcgcg
     3181 cccgtgatgt cgatcatgac tgaggatcgc gacctgctgt cgaacggtct ttattcgaga
     3241 tctttcggcc gctcgcccgg cgaagtgctc ggttatacga ccgtctacat gcgcggtctc
     3301 cagggcacag gcatgctcgg gtgtctcaaa cactttccgg gcatcggcgc aggcgaagtc
     3361 gattcgcacg aagagatgcc ggtggtacgt ctctcgcacg acgatctcat cgcacaggat
     3421 ctcgcaccgt acatcgagct ctttcagcgt cgcgacgatc gcgtacgctg cgtgatggtg
     3481 agtcacggcg gcttcccaaa cattgatatc aagggagaaa tgaccggcgg tttactcgaa
     3541 ccagcttcgt tgaactacaa catcgtgacg acgctgctgc gacaggagct cggctacaag
     3601 catctcgtcg tgactgacga cctcgagatg ggcgcgatcg cgcggcactg cgagattgaa
     3661 gacgctgtgg tacgtgcgac actcgcaggg gaagacatgc tgctgatttg cgcgagtcct
     3721 gacaaaatac gtcgcggcta tcacggactg ctcgaagcgg ccagcagtgg aaaaattaca
     3781 gaagagcgta tcaaacaaac gctcgaacgg atcgcgagga cgaagacgat catggagccg
     3841 ccagtgccac tcgacacggc gcgcttccag acgctcgcag acgaaatcct caaactcaat
     3901 tccaagctca actacaccta tggaggcacc gttaagtgag caagggcaag cttttactgc
     3961 tgctcgtttt gacattgctg ttgattattc ccccggcttg ccgccggcgc agcaacgcgt
     4021 ttgtgatcgc gctcggcgac aacgtccgca ccatcgaccc gattggtgcg cctgcggtcg
     4081 aggcggccag cgaacgcgtg cgcacgctga tgttcaacac cctcgtcaag aaggacgata
     4141 agtttgatta cgttggcgag ctcgcgtcag acatcaagcg ctccgatgat ggtctaagct
     4201 acacgtttac ccttcgcgat ggtgtgaagt ttcataacgg gcaagcgctg agctcggcgg
     4261 acgtgaagta cacgctcgat ctcgtcttct cgagcaactt cgggaagtcg gcgagttttt
     4321 ataaaggaca gggcgcggaa aagcgcagtt atatcaagag tgtcgaggcg cctgacgcgc
     4381 gcacggtggt cgtgacgttg accgagccgt gggttggatt gctctcgaat cttgttccga
     4441 tcccgatgat cccgaaagac agttacgagt cacaaaagac gcatccgctc ggcactggtc
     4501 cattcaagtt tgtgcattac gacaacgcgc agcaggtttg cgacgtcgag gcgtttccgg
     4561 attactggga aggcgcgccg aagctgcagt cggtgcgcgt acgtgtgatc tccgacatga
     4621 atgcgttaca ggcggaactg cagtcaggcc gcgttgatat tgccccgctg ccgacaagtc
     4681 tttcacccga tgcagtgaag cgactcgagg gcgatccgaa cctgcaagtg aaggttttca
     4741 acggttcgaa tgtcaatttg ttgacgatca atacggcgtc accgccgctc aacaatgtga
     4801 aggtgcgtca ggcaatcgct tacgcaatcg atcgcgagag tttgatcaag aacctgctgt
     4861 tgggactcgg aaagatcgca aactcgatca ttcccgagga gtcgtgggcg tattcgccag
     4921 ggcagacgta ttcgtacgat cccgcgcgcg cgaagcagtt gctcgatgaa gcaggatttc
     4981 gtgatcccga tggtgatgga ccacgaatgc ggttcgataa gccgatcgtc ttcaagcttt
     5041 ccggaagcag tattgcgggg cgacagtatg cgggtgtgat tcagaattcg ttgaaggaag
     5101 tcggaattcc ggtggagatc gagacggcgg aacagaatac atttcttgac gagctgaagc
     5161 gtggcaactt ccagatgtcg tattcgcagt gggtgggtgg aaatcaggat ccgattttct
     5221 acaaagatct tttcgcgagt tccgagatcc cgacagagac gcggtcggcg cgcaatcgca
     5281 gccgctacag taataaagat ctcgatgcgt tactgaatga agcactgaac acgtacgatc
     5341 atcaaaaagc tcgcgaactc tacgtgagga ttcaggagat cgtgagccgc gacgtgccgg
     5401 tgttcccgtt gtggtaccag tcaaacatcg tgatcgcgcg gaaggatgtg cagaacattc
     5461 aggtgaacgc gagcggcgac tggggattcg tccgcaatct aagccgttag tacacaaaag
     5521 gcacatagaa gaacacaaaa ggcacaaaaa ttttcttgtg ctttttgtgt tcttttatgt
     5581 gctttttgtg ggctaaaact ccgtgatttt gagcgaggcg gtggctgaga gaggcggggt
     5641 tactccggcg ttcaaataat cccacgccgc caatccgatc attgcggcgt tgtcagtcga
     5701 ccacttcagc ggcggcagac agagtttcac tcccattcga tcgcagagcg ccgcggcgcc
     5761 ttcgcgtagt tgcgggctcg ccgctacacc accaactacc accaaacatt ccgccggata
     5821 ttcttccagc gcgcgccggc acttcgtcaa cagtatctcc atgcatgagc tcacgaacga
     5881 cgccgccacg tccgcgtggt ccacgtcggg attcttttcg aggaaccttc ccaccgccga
     5941 cttcaacccc gagaatgaaa actcaaaacc ttcactcaac atgggacgcg ggaatggaat
     6001 cgaatctctt cccgtctttg caagcttgtc gaccgccggc ccacccggat atccgagccc
     6061 cagcagtcgc gcaaccttgt cgtaagcctc gccgactgaa tcgtcccgcg tcgagccgag
     6121 cagttggatc gatgtcagac tctccatgag cgccagaaac gtatggccac cggaaacgag
     6181 caacacgatt gccggtagct ccactcgctt ctcaacgaga tcggcgctgc gcaaatgccc
     6241 gcgcaggtga ttgattccga gcaccggaac gttccagccg agcccgagtc ctgccgccgt
     6301 attcacaccg acaatcaatg atccgatcag acccggaccg cgcgtcacgc caatggcttt
     6361 caaatccggt ccttcaacat ttgcttcttt cagcgcgcgc tcgatcgtcg gcacgaccac
     6421 cagtgcgtgc gcgcgactcg caaactccgg atacacgcca ccccagtgcg aatgaatatg
     6481 cgtttggctc gcgatgattg acgacaacac atttccgtcg cgatcgacga ctgcagcagc
     6541 cgtgtcgtcg cacgacgatt cgattcccag gatcacgtct tgactcatgc taaatacctt
     6601 aacacttcag attcgggcac gacgccgatg cgctcgattg caggcggatc gatgcgacag
     6661 ttcacgagcg tcgatgcagt cgtgcgcagc gttccgccat cgatcgaaag atccggcgca
     6721 ccgtcgagtt gcgcgagaat gtctgcgacg ttgtcaggcg tttccgcgct gtgtgcattc
     6781 gcgctcgtca cgagcaaagg cccggtcttg cgtaaaacgg aaagcagtcg cttgtcgttt
     6841 ggtatacgca ccgctgcttc gtcgcgtcct gctaaccacg ccggcctgta atcagagcta
     6901 aaccccatcg cgagcgtcaa actgcccggg atcaatggcg accgtagtaa tcgtctcgct
     6961 gactcgctaa cttcgaaacc aagcgcctcg agctcagcat ccgacgcgac catgatcgga
     7021 aggttcacat ttcgcgggcg gcgcttgagc gcaaacaatc gatctataga ctcatcaaac
     7081 tccggactca ccgcgagccc ataaaccgtg tctgtcggaa taagcacgac accaccgttt
     7141 ccaaggcact caactatctc attaatcaat ccgtctttca tccgtataat ccgcgtttaa
     7201 gttttactca aaaaacacgt ccgattggtc attgagcgcc tcaacttgcc tcgtcgatcg
     7261 cgcgccgttt cgcgaagcca gttgcaacaa cgtgcgcgca tccggattct cgattcccgt
     7321 atcgaccact cgtgcctctc ccaacaacgc cgcgagccgg tgtcgaatcc ttcccgccac
     7381 cgcgacatca ccgagcccgt cgctaacctt cgccagcgct tcagacacac gaccgaatcg
     7441 catgatctca accgactcgc ccctgatcaa gccaacgtac gcgttatcat tcgaggccgg
     7501 cactgcacat acaaccggca gctccgtcgt ctcacgagtt tgctcagcaa tgatctcaaa
     7561 ccagttgaac gaatagatgt tcacgttcaa gctaaacgcc agcgcattca caaacgaaat
     7621 tcccgcacgc acaaacgtca gcgaacctgg tccaacattg atcgccacac ccttgatctc
     7681 gctcgcggtg gcgccaatcg attcgagccc gcattcaagc aacgcgctca gagttctcag
     7741 cgattgatcg caatcattca cagccgaatc gaagatcgac tcaccatcac gtccaagcac
     7801 gacgcgatat acattcgaag acgattcgat tgccaacaac aaactcatac ctggttcttc
     7861 aatgcaacga gcaccggttg ccactgctcc gccgtcgcgg aaaacgtcaa cctgcgcgag
     7921 ttgtcctcgt tctcaacaaa ctcaaaatcg atcgacagcg cggtcgggaa ttccggctcg
     7981 acgatgtcgc cccactcgac cgccgtgatg ctctcagcca cgaaatcttc gagggccaga
     8041 tcgcgaaact ccgcaacact cgaaagccgg taagcatcga tgtgcaggaa cgtgcccgta
     8101 gggatcggat aaaactgtgc gatcgtgaaa gtcggactcg tgacttcggc agtggatccg
     8161 agcgccgcca ccagctcctg gacaaacgcc gtcttgccac tcgcgagcgg ccccttgagc
     8221 atcatcgcat cacccggccg caaaagaggc gcgagcgccg cggcaac
//
LOCUS       DASSFQ010000077         9580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1522294, whole genome shotgun sequence.
ACCESSION   DASSFQ010000077 DASSFQ010000000
VERSION     DASSFQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522294"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFH96_02390"
     CDS             <1..946
                     /locus_tag="VFH96_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6972841.1"
                     /translation="SDLMVVEADESDRSFLMLTPTIAVVTNIDREHMDYYHDMDDVRT
                     CFAKFVNSVPFYGSSVLCLDDPNVQAIIPLLERRRMTYGLSAQADVSAHGIRYNNNFG
                     STFDVWRGSDVIGELSLRVPGMHNVYNSLAAIAVGFELEVPFDKIVEGLSSFTGAGRR
                     FQAKGEVNGVLVVDDYGHHPTEVRATLAAAKLGSGGRRIVVLFEPHRYTRTHDLMQEF
                     ARSFNNADVLFISDIYAASEDPIEGVTAEALTAAIKRFGHKDANYIGSLDNAATVMRE
                     HVQPGDLVLTLGAGTVNRIADQLLGLLKEKEIGHGSNG"
     gene            924..1934
                     /gene="murB"
                     /locus_tag="VFH96_02395"
     CDS             924..1934
                     /gene="murB"
                     /locus_tag="VFH96_02395"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET6972842.1"
                     /translation="MATDLTDKNFTDRDHAVEEIVTRLGVRAERGKRFADLTSLRVGG
                     AIDWVITPETEEQAAAIVYELDKAGIGWRPLGSGSNVLADDADHHYIVLNLSNMKGEV
                     HIDGELVSVSAGYSLPRLCIDVARAGLGGIEGLGGIPGTVGGALWMNAGAFDHEIGTV
                     TEKVRVAREGKVVEIPGDSIEWNYRHTSFREGELLLGATLRLKPDDPEQIKARMDDAK
                     SRRLATQPHGSRSAGCFFKNPPASTIGTGKIIDEMGMKGQRRGSAVVSPVHANFIVTE
                     GENARAEDALALAEEIRERVRREQGIELEYEVELWQANPSTDYSEKTSVKSVESADRK
                     EC"
     gene            1938..2864
                     /locus_tag="VFH96_02400"
     CDS             1938..2864
                     /locus_tag="VFH96_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET6972843.1"
                     /translation="MREQVVAQKVGNRSGIGKSKSGVGQRPAKRGSNENISARLRSLL
                     TYVPLALRIGVIAIMALIAFVGYRAAASASFFQVRTIETRGASRASVDDIKGVVRRDV
                     SETGVWQADLQQLSTHLERLPWVRTAVVTRVLPDGIRIRITEREPKAVVRNGAGRFIW
                     VDDDAVSLGEMSPTDQMPAFFLRGWNEEDSNAARTENRDRVRKFLELQRDWGGQGISE
                     RVSEVNLLDLRDVRVQLAGDDSQIEVRLGSQEQSARLSKALSVLDAQRQTARGPLISY
                     IDLTQGKRAIVGLVTGNQLTEPSAVAPARPNN"
     gene            2879..4141
                     /gene="ftsA"
                     /locus_tag="VFH96_02405"
     CDS             2879..4141
                     /gene="ftsA"
                     /locus_tag="VFH96_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET6972844.1"
                     /translation="MAKRTQHTIGLDIGTSKVTCIAGEPGEGGLLEVVGIGEAEARGL
                     RKGVIVNPDAAVDAIKKAVEDAERMSGLEAEEVTINLAGSHILGFNGQAIVAVSGRDR
                     EIVAEDVRRAIDSACAIQLPAGREIVDRLPQEFIVDDQDGINDPVGMIGARLAVKVHI
                     VTSPVTARQNAINAVNRAGLVVADMVLEQLAAAEASLTEDDKEFGAALVDVGAETTGL
                     IIYQRGAVQHTAVFPLGGSHFTNDIAFGLRTPIPEAEKIKRTVGCACSSSLSEMERGE
                     LVDVPSVGGRPPRQLSRQILCDILQPRAEEVLMHVADEIKDAGWERQLSSGVVLTGGG
                     ALLHGMTEIAEQVFDAPVRIGYPERDRFGGLVEDIQSPAWTAAAGLALLAHRTQAAEI
                     RTGVSRRGSTGKLTHFVSRFKNRFGSIF"
     gene            4275..5429
                     /gene="ftsZ"
                     /locus_tag="VFH96_02410"
     CDS             4275..5429
                     /gene="ftsZ"
                     /locus_tag="VFH96_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET6972845.1"
                     /translation="MTPDTRQPGTGINIFIDDDPPLTGARIKVIGIGGGGSNAVNRMI
                     EAGIEGIEFLVANTDLQALKRSRAPIKIQLGSRLTKGLGAGADPNVGREAALEDTDKI
                     IEVLEGADMVFVTTGLGGGTGTGSAPIIASLATELDALTVAVVTKPFHFEGRRRMQQA
                     DNGLRELRECVDTVITIPNERLLHTVDRGVSLADSFKVADDVLRQAVQGISDLITVPG
                     LINLDFADVKSIMQGMGMALMGAGRATGENRAIEATQQAISSPLLEEATIQGAKGVLI
                     NITGGPDLTLFEVNAASSIIRESADDDANIIFGAVIDETLTDEMKITVIATGFDREIT
                     SVASAAATTAIPAPPPRYVARPADDLPRPMMGSPRVDDLDVPTFIRKKAD"
     gene            complement(5508..5729)
                     /locus_tag="VFH96_02415"
     CDS             complement(5508..5729)
                     /locus_tag="VFH96_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972846.1"
                     /translation="MFRTVSKLSQAAAHPRRGTNYTRNGISCKECRTCYTTKEEKAKE
                     KNLAAVKDLDARNAASKGAAAGVIACSKK"
     gene            5808..6206
                     /locus_tag="VFH96_02420"
     CDS             5808..6206
                     /locus_tag="VFH96_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972847.1"
                     /translation="MNRKLIRTTLAEVKGKARELPQPRERSNGSSRASNEGREQRAPQ
                     QHTPVQVKKRIPPPNETNAEIFYYKKQIDAHTVMVIVLLDGEEIEGTIEWYDRGALKV
                     NRKGAPNLLVLKRNIKYIYKAEERVESASA"
     gene            6260..7327
                     /gene="pdxA"
                     /locus_tag="VFH96_02425"
     CDS             6260..7327
                     /gene="pdxA"
                     /locus_tag="VFH96_02425"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET6972848.1"
                     /translation="MRHSEQSPTTSGPRPRRVLPRIGITMGDPAGIGPEVVLKAVAEE
                     EIRRVCVPVIIGDAQLLAHTARTLDLQSGYDIIRYEESFPDDFSEPVIYHLDNISGFI
                     EPGIESGAAGKAAAGYIEAAVELCAAGSIDAIATAPINKRALFLGGYSFPGHTEFLAH
                     LTGAEEYAMAFVAANLRIVLLSTHVPLAEAIRMVERDRLVKVISLTHRELQRWGIERP
                     RVAVAALNPHGAEGGLFGVEEASEIMPAIEASRRIDDINVQGPFSADTVFLRASRGEF
                     DAVVACYHDQAMIPVKCLSFGEAVNVTLGLPFIRTSVDHGTAFDIAGKGLAEHSSMVA
                     AIKLAADLSMQAGESGRAVEI"
     gene            7347..7760
                     /locus_tag="VFH96_02430"
     CDS             7347..7760
                     /locus_tag="VFH96_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6972849.1"
                     /translation="MENNDTLTNDNRRMLVADDDPAILRLIATILEKENFNVVTARDG
                     REAYKILQADPNFTAAILDVVMPHISGPELVRYMKSEERLKRIPVMMMTAEQDPKLSS
                     DSFSAGAVVFLPKPFTTAQLQIMLQMLIGKKTTDS"
     gene            7925..8710
                     /locus_tag="VFH96_02435"
     CDS             7925..8710
                     /locus_tag="VFH96_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HET6972850.1"
                     /translation="MLGWSLPGGAVRIQSIRSSGAESAEVSAEIQTVFRLRLNEGHWQ
                     LREIRTAPDRWEQLEVIARAAGAELPAGACDAPSEFARSKSETELTTKRARCLVASLF
                     GIGVPSDDVRIKEISPFGLSLGSESATLVEAFVRADFRLARDGKVWRVAEFKSGNRAW
                     VNVAGLAAAIDQVKRTAATDELSTIAKALGDFRRERGSFVVSESESVLIDHLSPRYLT
                     RVIRVDPWHRPYKYEGQPDHYSLRSVGPDGKPNTPDDIVVSGP"
     gene            8858..>9580
                     /locus_tag="VFH96_02440"
     CDS             8858..>9580
                     /locus_tag="VFH96_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HET6972851.1"
                     /translation="MCHRVGLRSSLRITLVLFIAVTLLPVPCVSLLVSAMGQGQGRGP
                     RTDPPPRPGKPEGALPDLDDMKHESSIKREPPAPIPSTIRSQRNSGKPWDGRRVGDPP
                     RDSDHAGGRGQTLRSHARRRMRVLPLLYEDQFTQNFFSVALGRSATSEETPYWNYQLR
                     AGYNESAASLNFAAIELGRTLFESAAYAARGRDAHGYVYDLYKTYLMRDPDAGGWATW
                     EGLVNTHGREYARRGFEESGEFA"
BASE COUNT         2131 a         2483 c         2880 g         2086 t
ORIGIN      
        1 cagcgatttg atggtcgtcg aagccgatga aagcgaccgc tcgtttctga tgcttacgcc
       61 gactatcgcc gtcgtgacca atatcgatcg cgagcatatg gattactacc acgacatgga
      121 cgatgttcgt acctgctttg caaagttcgt gaactcagtt ccgttttatg gttcctcggt
      181 cttgtgcctt gacgatccaa atgttcaggc aatcattccg cttcttgaac gacgcaggat
      241 gacctacgga ttgagcgcac aagcggacgt atccgcacac ggcattcgct acaacaataa
      301 ctttggatca acgttcgacg tctggcgcgg aagcgatgtt attggcgaac tgagcctgcg
      361 tgtaccgggc atgcacaacg tctacaactc acttgccgct atcgcggttg ggtttgagct
      421 ggaagtgcca ttcgacaaga ttgttgaagg actcagcagc tttacaggag caggacgaag
      481 attccaagcg aagggtgagg tcaacggcgt gttggttgtc gacgactatg gtcaccatcc
      541 gactgaagtt cgtgcgacgc ttgcagctgc aaaactcggt tcgggaggtc ggcgcattgt
      601 ggtgctcttt gaaccacatc gctacacgcg aacgcacgat ttgatgcagg agtttgcccg
      661 ttcattcaat aacgccgacg tattgtttat ttcggacatc tacgccgcga gtgaagatcc
      721 gattgagggc gtcacggcag aagcgttgac ggcggccatc aagcggttcg gacataagga
      781 cgcgaactac atcggctcgc tcgataacgc cgccacggtt atgcgcgagc acgtgcagcc
      841 gggcgatctc gtgttgacgc tcggtgcggg cacagtgaat cgaattgccg atcaactgct
      901 cggattgttg aaagaaaaag aaattggcca cggatctaac ggataaaaat tttacggatc
      961 gagatcacgc agtcgaagag atcgtgacgc ggttaggtgt gcgtgcggaa cgtgggaaac
     1021 ggttcgctga tttaacaagc ctgcgcgtgg ggggtgcgat cgattgggtc attacaccgg
     1081 aaacagaaga gcaggcggcg gcgatcgttt atgaacttga taaagcgggc attggttggc
     1141 gaccgctcgg ctccggcagc aacgtactcg ccgacgatgc cgatcatcac tacatcgttt
     1201 tgaacttgag caacatgaaa ggcgaagtgc acatcgacgg cgagcttgtt tcggtcagcg
     1261 cgggttactc gctgccgcga ctctgcatcg atgttgcgcg cgccgggctc ggcgggatcg
     1321 agggactcgg cggcattccc ggcacggttg gtggcgcgct ctggatgaat gccggtgctt
     1381 tcgatcacga gatcggaacc gtaaccgaga aagtgcgcgt ggctcgcgaa ggtaaggtgg
     1441 ttgagattcc cggtgacagt atcgaatgga actatcgtca cacttcgttt cgcgaaggcg
     1501 agttgttgct gggtgcgacg ctgcgtttga agccggacga tcccgaacaa attaaagcgc
     1561 gcatggatga tgcgaagagt cgtcgcctcg cgactcaacc gcacggctcg cgttccgcgg
     1621 gttgtttctt caagaaccca ccggcaagca cgatcggcac cggcaagatc atcgatgaga
     1681 tgggaatgaa agggcagcga cgcggcagtg cagtggtctc gccggtacac gcgaacttca
     1741 tcgtcactga aggtgaaaac gcgcgtgccg aagatgcgct tgcgctcgcg gaagagattc
     1801 gtgaacgcgt acgacgcgag cagggaatcg agttggaata tgaggttgag ctgtggcaag
     1861 caaatccatc cacagattac tcagagaaaa catctgtgaa atctgtggaa tctgcggata
     1921 gaaaggagtg ttgagcgatg agagaacagg tcgtggcgca gaaggttggt aatcgtagcg
     1981 gcatcggcaa gagtaagtca ggtgtgggcc agcgtccggc gaagcgtgga tcgaatgaaa
     2041 acatcagcgc gcggctgcga tcgctgttga cttacgtgcc gctcgcgctc cgaatcggcg
     2101 tgatcgcgat tatggcgctg attgcgtttg tcggataccg tgcggctgcg tcggcaagct
     2161 tctttcaggt gcgaacgatc gagactcgcg gcgcgtcacg cgcatcggtt gatgacatca
     2221 agggtgttgt acggcgcgat gtgagcgaga ccggcgtgtg gcaagccgat ctgcaacaac
     2281 tcagcactca tcttgaacgt ttgccatggg tgcgcacggc agtcgtcacg cgtgttcttc
     2341 ctgacggcat ccgcattcgt attaccgagc gcgagccgaa agcggttgtg cgtaacggag
     2401 ccggacgatt catctgggtc gatgacgatg cggtttcact cggtgagatg tcgccgaccg
     2461 atcaaatgcc cgcgttcttc ctgcgcggtt ggaacgagga agactcgaac gctgcgcgta
     2521 ctgagaaccg cgatcgagtg cgaaagtttc tcgagctgca acgcgactgg ggcgggcagg
     2581 ggatttcaga acgtgtgagc gaagtgaatc tgctcgatct gcgcgacgtg cgcgtgcaac
     2641 tcgcgggtga tgattcgcag atcgaagttc gactcggatc gcaggagcag agtgcaaggt
     2701 tgagtaaagc gttgagtgtt ctcgatgctc aacgtcagac ggcacgcgga ccactgatca
     2761 gctacatcga tctcacacag ggtaaaagag cgatcgttgg gttagtaacc ggcaatcaat
     2821 taacagaacc gagcgcggta gcgccggctc gtcctaacaa ctaaagagag cccccgacat
     2881 ggccaagcga acacagcaca cgatcggtct cgacatcggg acaagtaagg tgacgtgtat
     2941 cgccggcgaa cctggtgagg gtggattgct cgaagtcgtc ggcatcggtg aagcagaagc
     3001 gcgcggtttg cgcaaaggcg tcatcgtcaa tcccgatgca gcggtcgacg cgatcaagaa
     3061 agcagtggaa gacgcagagc gcatgagtgg actcgaagcc gaagaggtca cgatcaatct
     3121 cgccggctca cacatactcg gtttcaacgg ccaggcgatc gtcgctgtct ccggtcgcga
     3181 ccgcgagatc gtcgctgaag atgtacgacg cgcgatcgat tcggcctgtg cgattcaact
     3241 tccggccgga cgcgagatcg tcgatcgact gccgcaggaa ttcatcgtcg acgatcagga
     3301 tggcattaat gatccagtcg gcatgatcgg cgcacggctc gcggtgaagg tgcacatcgt
     3361 tacgagtcct gtgactgcac gccagaacgc aatcaacgcc gtgaaccgcg ccgggctcgt
     3421 cgtcgcagac atggttctgg agcaactggc agccgctgag gcgtcgctaa ctgaagatga
     3481 caaagagttt ggagcggcac tcgtcgacgt cggcgcggag actacggggc tgatcatcta
     3541 ccagcgtggc gctgttcagc acacggcagt tttcccgctc ggtggctcgc atttcacgaa
     3601 cgacatcgct tttggtctac gcacgccgat tccggaagcc gagaagatca agcggacagt
     3661 cggctgcgcg tgcagcagct ctttgagtga gatggaacgc ggtgagctcg tcgatgtgcc
     3721 ttctgtcggt ggacgtccac cacgtcaact ctcgcgtcag attctctgcg acatactgca
     3781 gccgcgtgct gaagaagtgt tgatgcacgt cgctgacgag atcaaagacg cgggttggga
     3841 acgacagctt tcgagtggtg tcgtgttgac tggcggcggc gcgctgttgc acggcatgac
     3901 tgagatcgca gaacaggtat tcgatgcgcc agtgcgcatt ggttatcccg agcgagatcg
     3961 ttttggtggg ttggttgaag acattcagag cccggcatgg actgccgctg caggtctcgc
     4021 gctgctcgcg catcgcactc aggcagcgga gatacgaact ggggttagca ggcgtggatc
     4081 aacaggtaag ctgacgcact tcgtgtcacg ttttaaaaat agattcggta gtattttctg
     4141 acaagttgtt gtatgcttgc gccccggttg cacaagatat tgtccgccag atatcgaggc
     4201 cagtggctca agaaccccaa catagaagga agcggccttg gaagccatcc cgttttggta
     4261 gagaggaact aactatgaca ccggacactc gtcaaccggg cacgggcatt aatattttca
     4321 ttgatgatga tccgccactc acaggtgcgc ggataaaagt gatcggcatc ggcggcggtg
     4381 gcagcaacgc tgtcaatcgc atgatcgaag ccggcatcga aggtattgaa tttttagttg
     4441 cgaatacaga cctgcaggcg ttgaagcgtt cgcgcgcacc aatcaagatc cagctcggca
     4501 gtcgtttaac taaaggattg ggcgcgggcg ctgatcctaa tgttggccgc gaagcagctt
     4561 tggaagacac tgacaagatc attgaagtgc tcgaaggcgc tgacatggtg tttgtgacta
     4621 ctggtctcgg cggtggcacc ggaaccggct ccgcgccgat catcgcgtca ctcgctacag
     4681 agcttgacgc gctgacagtc gcagtcgtaa cgaagccgtt tcatttcgaa ggtcgacgtc
     4741 gcatgcagca ggccgataac ggactgcgtg agttgcgcga gtgcgtcgac acggtgatca
     4801 cgatcccgaa cgagcgtttg ctgcacacag tagatcgtgg cgtgtcactc gctgactcgt
     4861 tcaaagtagc tgacgacgtg ttgcgacagg cagtacaagg catcagcgat ctgatcactg
     4921 tgcctggtct gatcaatctc gacttcgctg acgtgaagtc gatcatgcag gggatgggaa
     4981 tggctttgat gggcgcgggc cgagcgactg gtgagaaccg cgccatcgaa gcaacccagc
     5041 aggcgatctc gtcaccgctg ctcgaggaag caacgatcca gggcgccaaa ggtgttttga
     5101 tcaatatcac cggcggaccc gatctgacct tgtttgaagt gaatgctgcg tcgtcgatca
     5161 ttcgcgagtc tgctgacgac gatgcaaaca taatttttgg cgcagtgatc gacgagactc
     5221 tgactgacga gatgaagatc actgtgatcg cgaccggttt tgacagagaa atcacatcag
     5281 tagcgtctgc cgctgcgaca acggcgattc ccgcgccgcc accacgttac gtagccaggc
     5341 cagccgacga cctcccaagg ccgatgatgg gttctcctcg tgttgatgat ctcgacgtcc
     5401 cgactttcat tcggaagaag gctgactaga gatcattttg ggcaggatta ctgcttttca
     5461 tggttgtcgc ggccgaaccg tcaatcatga aaatactgta atcctgtcta tttttttgag
     5521 catgcaatca cgcctgcagc cgcgcctttg cttgcagcgt ttcttgcatc taaatctttg
     5581 acagcagcaa ggtttttctc ctttgctttt tcttcttttg ttgtgtagca tgtccggcat
     5641 tccttgcagg aaattccatt acgggtatag ttcgttcctc tcctcggatg ggccgcagcc
     5701 tgcgagagct ttgacactgt tctgaacatc aaaacctgtg tcctatacat tggtagttct
     5761 cgactgggcg taaacgcccg tcatagattt ttggaggtaa taatcacgtg aatcgtaaac
     5821 tcatcagaac cacactcgcg gaagtcaaag ggaaagcccg agaacttccg cagcccagag
     5881 agcgttcgaa tgggagttcc cgcgcctcaa acgaaggcag agagcaacgc gctccacagc
     5941 agcacacgcc ggtccaggtt aagaaaagaa ttccgcctcc caacgaaacc aacgcggaga
     6001 tcttttatta caagaaacag atcgacgcac atacagtgat ggtcattgtt ctacttgatg
     6061 gcgaagagat tgaaggcacg atcgagtggt acgatcgcgg cgcgctcaag gtcaatcgaa
     6121 agggtgcccc caatctgctc gtgctcaagc ggaacatcaa atacatctac aaggctgaag
     6181 agcgtgttga aagtgccagc gcatagttaa tgcctcgccc tgagttctcg ccctggacaa
     6241 cagtgcgtcg cctcaagcaa tgcgacactc agaacaaagc ccaacgactt cgggacctcg
     6301 accgaggcga gttttgccgc gcatcggcat cacgatggga gaccctgccg gcattgggcc
     6361 ggaagttgtt ctgaaagctg tcgccgaaga agaaatcaga cgagtttgcg tgccggtcat
     6421 aatcggcgat gcccagttgc tggctcacac ggcacgcact cttgatctgc aatccggtta
     6481 cgacatcatt cgttatgaag aatcgtttcc ggatgatttc tccgaacccg ttatctatca
     6541 cctcgacaac atcagcggct tcatcgagcc gggtatcgag tcaggcgcgg cgggcaaagc
     6601 tgcggctggt tacatcgaag cggcggttga gctgtgtgct gcgggcagca tcgatgcgat
     6661 tgcgactgcg ccgatcaaca agcgcgcact gttcctcggt ggttacagtt ttcctggcca
     6721 tacggagttt ctggctcatc tcactggtgc cgaggaatat gcgatggcgt ttgtcgcggc
     6781 aaacctgcgc atcgtgttgt tgtcgacgca cgttccgctg gccgaagcaa tccggatggt
     6841 cgagcgcgat cgactggtga aagtcatcag tctgacgcat cgcgagctac aacgttgggg
     6901 catcgagcgc ccgcgagttg cggtcgccgc cttgaaccca cacggagctg aaggcggact
     6961 ctttggagtg gaagaggcgt cggaaatcat gccggcgatc gaagcttcac gccggattga
     7021 tgatataaat gtgcaggggc cgttctcggc agacactgtt ttcctgcgcg cctcgcgagg
     7081 tgagtttgat gcagtggtcg cctgttatca cgatcaggcg atgattccgg taaagtgttt
     7141 gtcgtttggc gaggccgtga acgtaaccct cggattgcct ttcatcagga cttcggtgga
     7201 ccacggcacc gctttcgaca tcgccggcaa aggactcgcc gagcattcaa gtatggttgc
     7261 tgcgatcaaa ctcgcggcgg acctttccat gcaggctggt gagagtggtc gcgcagtgga
     7321 aatttgaaaa cgggaagaac actcacatgg agaacaacga cactcttacg aacgataacc
     7381 ggcgcatgct cgtcgctgat gacgaccccg cgattcttcg tctcatcgca acgattctcg
     7441 agaaagaaaa cttcaacgtg gtgacggcgc gtgacgggcg cgaggcttac aagatcctgc
     7501 aggcggatcc aaacttcacc gccgcgattc tcgacgtcgt catgccgcat atttccggtc
     7561 ccgagttggt ccgttatatg aaatcggaag agcggctgaa acgaattccg gtgatgatga
     7621 tgacggccga acaggatccg aagctctcgt ctgatagttt ttccgcgggc gcggtcgtgt
     7681 ttctgccgaa gccgttcacg acggcgcagc tacagatcat gttgcagatg ttgattggta
     7741 agaagactac cgactcctaa tgctgccacg aaattttagc cacgaaaagg cacaaaaagc
     7801 acagaaagag ttaggaaaag ctttcgtgtt ttttgtgctt ttttgtggcc tgcttttaac
     7861 tcttggaggc atcgaggcgc gcgccgatat cagccagaaa aacgctcaca aagtgataca
     7921 gacgatgttg ggttggtctc tgcctggcgg tgccgttcgt attcagtcga ttcgatcgag
     7981 tggcgctgag agtgcggaag tcagtgccga gattcagact gtctttcgac tgcgattgaa
     8041 cgaagggcat tggcagttgc gcgagatcag aacggcgccc gatcgctggg agcagttgga
     8101 agtgattgcg cgtgcggcgg gtgctgagtt gccggcgggc gcctgtgatg caccttctga
     8161 gtttgcgcgg agtaaatcgg agacggagtt aacgactaaa cgtgcgcgct gtttggtggc
     8221 gagtttgttc ggcattggag tgccttccga tgatgtgagg atcaaagaga tctcgccgtt
     8281 tgggttgtcg ctcggatcgg agtccgcgac gctcgtcgag gcgtttgtcc gcgcagactt
     8341 tcgtttagca cgagacggga aagtttggcg agtggcagag tttaagagcg gcaatcgtgc
     8401 ctgggtaaac gtcgcggggc tcgctgctgc gatcgatcag gtcaaacgca ctgctgctac
     8461 cgacgaactt tcaacgatcg ccaaagcgct gggcgacttc cgtcgcgaac gcggttcatt
     8521 tgtcgtttcc gaaagcgaat cggtcctgat cgatcacctc agcccgcgct atctcacgcg
     8581 cgtaatccgt gtcgatccat ggcaccgtcc gtataaatac gaaggccagc ccgaccatta
     8641 ttcgctccgc tcagtcggcc ccgacggcaa acccaacaca ccggacgaca ttgtggtctc
     8701 cggtccataa atttttgccc tcctaaaatc tcattgactt ttggtccata ggaggagtag
     8761 agtgcgcgac actgctccca aaatagttgt cgcgcgagcc cccctgcgac gaagagtaaa
     8821 taaaaaaaca gtttagaaac tcacaggagt cctcatcatg tgtcatcgtg ttggtttgcg
     8881 gtcctcgctg cgaatcactc tcgtattgtt catagctgta acgctgttgc cggtgccctg
     8941 cgtttcgctg ctcgtcagcg caatgggcca gggtcaggga cgtggtccgc ggaccgatcc
     9001 accaccgcgg cccggtaagc ccgagggtgc gttgcccgat ctcgatgaca tgaagcatga
     9061 gtcgtcgatt aaacgcgagc ctccggcgcc gattccttcg acgattcgtt cacaaagaaa
     9121 ctcgggtaag ccctgggacg ggcggcgagt tggcgatccg ccccgcgact cggaccatgc
     9181 tggtgggcgc gggcaaacgc tacgctcgca cgcgcggcgg cgaatgaggg ttctgccgct
     9241 actctatgag gatcaattca cacagaattt tttcagcgtc gctttagggc gcagcgcaac
     9301 ttcagaagaa acaccttact ggaattacca gttgcgcgcc ggttacaacg agagtgcggc
     9361 gtcgttgaat tttgccgcta tcgagctcgg caggacgctt ttcgagtcgg cggcctacgc
     9421 cgctcggggc cgtgatgcgc acggttatgt ctacgatctt tacaagactt acctgatgcg
     9481 cgacccggat gcaggcggct gggcgacatg ggaaggtctg gttaatactc atggacggga
     9541 gtatgcgcgc cgcgggtttg aagaatcagg cgagtttgcg
//
LOCUS       DASSFQ010000078         3518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_773956, whole genome shotgun sequence.
ACCESSION   DASSFQ010000078 DASSFQ010000000
VERSION     DASSFQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773956"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFH96_02445"
     CDS             <1..801
                     /locus_tag="VFH96_02445"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET6972852.1"
                     /translation="KDEDRQALADVVDSIKLNTGDTLFHAGDPGESLFIVRTGSVELF
                     IKDTVGQKIVLTISEAGDLFGELSLLDSGPRTATAVALSDTDLLMLDRDDLLLLFEKR
                     PDSALNMLAAMSAMTRKADELLRTRVSRNVNEEMEEHSTPLQRIADWIAWFSGSMPFL
                     IINAAWFIVWISINTLHLGIPAFDPYPFGLLTMIVSLEAIFLSCFVLVSQNRQAEKDR
                     VRADIEYEVNVKAELEVAHLHEKTDRLYEGMLARFAKLEKMIQPGRVA"
     gene            878..1723
                     /locus_tag="VFH96_02450"
     CDS             878..1723
                     /locus_tag="VFH96_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET6972853.1"
                     /translation="MRWRGERQSTNIEDRRGMSVGKVAVGGGLGTIVIMILALLFGIN
                     PQQLFEQLPSDQPGVQSSRPTSPEEEELKQFVSVVLAKSEDVWTDVFRQNGRQYREPT
                     LVLFTDRVQSACGITGAAVGPFYCPGDEKVYIDLSFYEQLRREFKAPGDFAQAYVVAH
                     EVGHHVQKLLGITDRIDSLRGRVGEVEANQMSVRLELQADFFAGVFAKYVQNQGMLEA
                     GDIEEALRAASAVGDDQIQRRTAGYVVPDSFTHGTSEQRLRWFKKGFETGDIRQGDTF
                     GARTL"
     gene            complement(1759..2496)
                     /locus_tag="VFH96_02455"
     CDS             complement(1759..2496)
                     /locus_tag="VFH96_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972854.1"
                     /translation="MSLFRALNAEVLKLKRTLAFRVIFVLPLLIAVLQFFIILRTKKY
                     APGFNLWQTHPTNSFQIWAVFMMPLLIALVTALLNGIEHSDKQWKHLFALPVPRSAVY
                     FAKLMVAQGLILTSTLVLAVFTAIVGVVGMYLRPELANAGPVPYVWLAKQVAFIWLAS
                     WLIIAIHTWISMRWAGFPIALGAGIGGTFFALFAASAALGKYYPWLLPMNIFVEGRFA
                     MALLLGIGGGILAALLGCFEFVRRDVM"
     gene            complement(2496..3413)
                     /locus_tag="VFH96_02460"
     CDS             complement(2496..3413)
                     /locus_tag="VFH96_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972855.1"
                     /translation="MTNNYLIETRGLTRRFGSQRAVDDLNLLVPAAGVYGFLGPNGAG
                     KTTAIRMLLGLIRPNAGDVQLFGQSLNGNHRSLMRRVGALVEAPSLYPHLTGRENLEV
                     TRRLLGSDRNLVELALATVKLTKDADRRVREYSLGMRQRLGLALALLNNPDLLILDEP
                     TNGLDPAGIHEMRDLIRRLPDEFGVTVFLSSHLLGEVEQIATHIGIIHEGSLLFQGTL
                     AELQSKQQTQLIVGVKQLEAATEFLVNTGWSVQRCVDGLLNVSTKAADDAMRINKLLV
                     DRGLDVFHLAVSQRSLEDIFLSLTNGRSS"
BASE COUNT          852 a          978 c          957 g          731 t
ORIGIN      
        1 aaggatgagg atcgacaggc gctcgcagac gtggtcgatt cgataaaact gaacactgga
       61 gacacactct ttcacgccgg cgacccgggt gaatcgttgt tcattgtccg taccggttct
      121 gtcgagctat ttattaaaga cacagtgggc cagaagatcg tgctcaccat ctctgaagca
      181 ggcgatctct tcggcgaact ctcgctgctc gatagtggtc caagaactgc gactgctgtt
      241 gctttgagtg atacagactt gctcatgctc gatcgcgacg atctgttgct gctctttgag
      301 aagcgacctg actcggcgct caacatgctc gcggcgatga gcgcgatgac gcgcaaggct
      361 gatgagctgc tacgcacgcg cgtctcgcgc aacgtcaacg aagagatgga agagcacagc
      421 acgccgttgc agcgcattgc cgactggatt gcgtggttca gcggcagcat gccgtttctg
      481 atcatcaacg ccgcgtggtt catcgtctgg atctcgatca acactcttca tctcggtatt
      541 ccggcattcg atccgtaccc gtttggtctg ctgacgatga tagtttcgct cgaggcgatt
      601 ttcctgtcgt gcttcgtact cgtcagtcaa aaccggcaag ctgaaaaaga tcgcgtgcga
      661 gcggatatcg aatacgaagt gaacgtgaaa gcagagctcg aggtcgcgca tctgcacgag
      721 aagaccgatc gtctctacga aggcatgctc gcgcgcttcg ctaagttgga gaagatgatt
      781 cagccgggca gagtggcgtg actaaaaaac aattccgctg acacaaagcg caggccgttc
      841 tcgtcgacaa tacactccat accggaggag cgaaactatg cgttggcgag gcgaacggca
      901 aagcacaaat attgaggatc ggcgtggtat gtccgtcggc aaagttgcgg tcggcggcgg
      961 cctcggaacg atcgtcataa tgatcctcgc gctgctattc ggtattaatc cgcagcagtt
     1021 gttcgagcaa ttaccttccg atcagccggg cgtgcaaagc tcgcgtccca ccagtccgga
     1081 agaagaagag ctgaaacaat ttgtctcggt ggtcctggcg aagtcggaag atgtttggac
     1141 cgacgtgttt cgtcagaacg ggcgacagta tcgcgagcca acgctcgtgc tctttaccga
     1201 tcgagtgcag tctgcttgtg gaattacagg cgctgcggtt ggtccattct attgtccggg
     1261 tgatgaaaag gtttacatcg atctctcgtt ttatgaacaa ctgcgacgcg agttcaaggc
     1321 gccgggagat tttgcgcagg cgtacgtggt cgcacatgag gttggacatc acgtgcagaa
     1381 gcttttagga atcaccgatc gcatcgattc gttgcgagga cgagtgggtg aagttgaggc
     1441 gaatcagatg tcggtgcggt tggagttgca ggctgatttc tttgcgggcg tgtttgcgaa
     1501 gtacgtgcag aaccagggaa tgctcgaggc cggtgacatc gaagaggcgt taagagcggc
     1561 gagtgcagtt ggtgacgatc agatccagcg acgcacggca ggatatgttg tgcccgactc
     1621 cttcactcac ggaacctcag agcaaaggtt gcgctggttc aagaaaggat tcgaaaccgg
     1681 agacatccgc cagggagaca cattcggcgc ccgcacttta tagtaaaaat ccgtgttttt
     1741 aatccgtgtt atccgtggct acataacatc tcggcgcacg aactcgaagc aaccaaggag
     1801 cgccgcaaga atcccccctc cgatacccag cagcaacgcc atcgcaaatc ttccctcaac
     1861 gaagatgttc atcggcaaca gccacggata atacttgccg agcgctgcac tcgccgcgaa
     1921 gagcgcaaaa aacgtcccac caattcccgc gcccagcgcg atcggaaacc cagcccagcg
     1981 catactgatc cacgtgtgaa tcgcgatgat caaccaactc gccagccaaa tgaacgcgac
     2041 ctgcttcgcc agccaaacgt acggcaccgg tccagcattc gcgagctcag gtcgcaagta
     2101 cattccgaca acaccaacga tcgcggtaaa cactgccagc accaacgtgc tcgtcaaaat
     2161 caatccctga gccaccatca acttcgcgaa gtacaccgca gagcgcggca cgggcagcgc
     2221 gaagagatgt ttccactgct tgtcgctatg ctcgatgccg ttgagcaacg ccgtgaccaa
     2281 agcgatcagc agcggcatca tgaacacggc ccagatctga aacgagttcg tcgggtgcgt
     2341 ctgccacaga ttgaatccgg gcgcgtattt cttcgttcgc aagatgatga aaaattgaag
     2401 caccgcaatc aacagcggca gcacgaagat aacgcgaaac gcgagcgtgc gttttagctt
     2461 caaaacttcg gcgttcagcg cgcgaaacag cgacatcaac tcgatctccc attcgtcagg
     2521 ctcagaaaaa tgtcttccaa cgatcgctgc gacactgcaa ggtgaaacac gtccaggcca
     2581 cgatcgacca gcagcttatt gatccgcatc gcgtcgtccg ctgctttggt cgaaacgttc
     2641 agcaaaccgt cgacgcaccg ctgtacactc caaccggtat tcaccagaaa ctctgtcgcc
     2701 gcctcgagct gcttcacgcc cacgatcaat tgtgtttgct gtttcgactg taactcagcc
     2761 agtgtgcctt gaaacaacaa actgccttcg tgaatgatgc cgatgtgagt agcgatctgt
     2821 tcgacctcgc ccagcaagtg actcgaaaga aaaaccgtca cgccaaactc gtcgggcaag
     2881 cgcctgatca agtctcgcat ctcgtgaata cccgccggat cgagaccatt cgtcggctcg
     2941 tcgaggatca gcaggtcagg attgtttagg agggcgaggg ccagtccgag tcgctggcgc
     3001 ataccgagcg agtactcacg cacacgacga tctgcatctt tcgtgagctt gacagttgcg
     3061 agtgcaagct cgaccaggtt gcgatccgaa ccgagaagac gtcgtgtgac ctctaggttc
     3121 tctcttccgg tcagatgcgg atagagcgac ggcgcttcaa cgagcgcgcc gacgcgacgc
     3181 atcaacgagc gatgattgcc gttgagcgat tgaccgaaca gctgcacgtc gccggcgttc
     3241 ggcctaatca gaccgagcaa cattcggatc gccgtcgtct tacccgcgcc atttggacca
     3301 agaaagccat agacacctgc tgcgggtacg agcaagttca aatcatcaac agcgcgctgg
     3361 gatccgaaac ggcgcgtcaa cccgcgcgtc tctatcaggt agttatttgt cactgaacaa
     3421 taaacgcacc cgaacccgat tcattaactt agccacaaaa ggcacaaaaa gcacaaaagt
     3481 ctgtgtttgt gctttttgtg ccttgtgtgg ctaatcag
//
LOCUS       DASSFQ010000079         7327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_334065, whole genome shotgun sequence.
ACCESSION   DASSFQ010000079 DASSFQ010000000
VERSION     DASSFQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334065"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1358
                     /locus_tag="VFH96_02465"
     CDS             96..1358
                     /locus_tag="VFH96_02465"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6972856.1"
                     /translation="MSRPTLVQRFITVGAIFLLGSAAIQAQTTRPRRTNRQPAQTTKT
                     PADDPLLKPEPQPSPTARRSSSNDSLLDVQPVRPVANTVASADTKHAFLLLDQKQFAA
                     ALKEAKELTASYPNDPEAWKIAGFAELNLKQYAEAVKDLEKAVELQRNAKQDDPNTES
                     ALGQAYVLSEKYQEALPLLTAAVNRNPAQPDSMLIYYRGISEYKTGKIADAEKSFNQV
                     LKLNPKDSLSVFYLAQIALAKNDIDGAIASLNRATVNDPRMTGAWALLTTAYLRRAAT
                     STDQAKADADYISAVRAGEGLIKLRTDAEAVTLFGQALIGSKQYARAAGVLERATVAP
                     DATGVTFYLLGVAQSRAQNFPKAIAALESAAKKTPDDVNIYRELGYAYEVTKQYAKAL
                     GAYQKGSSLAPSDADLKESIERVKPFAK"
     gene            1361..1621
                     /locus_tag="VFH96_02470"
     CDS             1361..1621
                     /locus_tag="VFH96_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972857.1"
                     /translation="MSGLPGTARAFATYSLVPYLGILFCPGAVVLGAVGVVRSYRSQQ
                     VVGDSSRGICYASTIVGVVVTAVQLLLWWVLYQAPMWANQLN"
     gene            1637..2209
                     /locus_tag="VFH96_02475"
     CDS             1637..2209
                     /locus_tag="VFH96_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972858.1"
                     /translation="MKIISALLLLGALLTGAYAQRPRSMDPQPATTPATVARKPAPAP
                     KTVKAKYEGGVFGYNKTMDGTLTFDDANNRLIFKDKKPPKEISIPYEAITSAFADSKK
                     VQPAAATVAQNVPSIYSLPARFIKHNVRYLTLQYSDPDSKISGITSFKISDKELLESV
                     LVTLAEKTNMTLRGDVYVKKKPEDSSKATP"
     gene            complement(2301..2900)
                     /locus_tag="VFH96_02480"
     CDS             complement(2301..2900)
                     /locus_tag="VFH96_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6972859.1"
                     /translation="MSKNQHLPLAGSNKKTDARVRRTRDALGDALIALMQEKPFETIT
                     VQEVLDRAHVSRSTFYMHYSDKDDLLMSDAEEFFEALSMALSEHDDKSDRVFPVKEFF
                     NHLADVQPFYKALVKSGKFQENMELARGHFARGIERRLSELPRGKSIPTSERSAIAFT
                     HAGALLSLLAWWLDRGMRERPEQMDELFHRMVWNGLSKE"
     gene            2990..3673
                     /locus_tag="VFH96_02485"
     CDS             2990..3673
                     /locus_tag="VFH96_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972860.1"
                     /translation="MISLNHVSKTYETPAGKFAALKDIDLEIRPGEFVGVVGKSGSGK
                     STLLNMVAGIDRPSSGSVAVAGTEIHALSENKLAAWRGRNVGFVFQFFQLLPTLTAAE
                     NVMLPMDFSKTIPLRERRKRAVELLELVAVGSHADKLPATLSGGEQQRVAIARALANE
                     PPLVLADEPTGNLDSITATEILDLFRNMANRGTTVVIATHEADIARVIDRKIEISDGK
                     IHPHISQMT"
     gene            3677..6016
                     /locus_tag="VFH96_02490"
     CDS             3677..6016
                     /locus_tag="VFH96_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6972861.1"
                     /translation="MTTIWKKAIRDFWQERARTTLVVLAIALGISAFAAVLSSYAILT
                     RELDRGYLETNPASAVLRVDSIDKDLVKATLQNPEVSDAEPRRTIGGQIKAGPVQWRN
                     LILFVVEDYGNIRVSKLVPESGAWPPATGEILIERDAFQVAKVKIGDAVTVKTANGVE
                     TPLVISGSVHDVGQAQARMENIVYGYINLATLVQLGEEPRFDQLNILVAQNRFDEDHI
                     RHVASDVEKLIEAHGHDVRRVEIPRPGKHPHSDLTGILLLAMSSFGLFVLVLSGILVI
                     NLLTAMMASQVRQIGVMKAIGGTRWAVARIYFAQALFLGIAAVLVSIPLGILGSRALC
                     RYMAIFLNFDINSFAVPIWVYLLVALIGVIAPVVAAAYPVWRGTKTPVKVALSDFGLS
                     QTNFKTGAVDRAVAQIGGPFRLLAFAIRNSFRRRARFVLTLLTLAAGGLFFLSALNVR
                     ASMVNTLDRMFAARKSDLSVSFAKPYDFEKIQKAVSNTAGVKRAEGWFNGEGSLGERR
                     FGVVALPADTQLLDFDIIEGRKLTPQDTDAIVINNALAGREPQMHVGETVALRIGAEE
                     KTWRVVGIAHEAFAPSPVAYIPLTGMVNSLRLALEDTDPDAVGALKTELDRNLEAQGV
                     RARGSSTKSESRFGFDQHMVMIYMFLIVMSAIVGGVGGLGLMTTMSLNVLERRREMGV
                     MRALGATPRIVWLMIVAEGVVIGLLSWTIAALLAWPVSKAIGDSFVRMLFHSGLDFTF
                     EPLGLVIWMLVSILLSAVASFLPAWKASRTTVREALAYE"
     gene            complement(6067..6978)
                     /locus_tag="VFH96_02495"
     CDS             complement(6067..6978)
                     /locus_tag="VFH96_02495"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET6972862.1"
                     /translation="MASNVVQNPLELDSLLASSRALVELELNKLLPLSGTAPEKIHQA
                     IRWSVFAGGKRFRPALLLAVGQAFSAPVEALIRTACAFEMVHTYSLIHDDLPAMDDDE
                     LRRGRPTCHVRFDEATAILAGDALQTLAFQTIAEDVALDAETRIALIAELARAAATPD
                     GMVAGQALDMEAETRQVSRAQLEEIHRLKTGALIKAAARCGAIIGRADESELTAVTSY
                     GSHLGLLFQITDDFLDVTATAEDLGKTPGKDARSNKATYPALHGLEETRAAAINAHRD
                     ACHALDQLDRSTDVLQKIADFILHRTG"
     gene            complement(6968..7231)
                     /locus_tag="VFH96_02500"
     CDS             complement(6968..7231)
                     /locus_tag="VFH96_02500"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET6972863.1"
                     /translation="MSNGAEQSRTFETSLEALEQIVRQLEDGDLPLEKSLELFEQGIR
                     LSRECQERLSQAERRIEILLRDNQGRPVAAAFKEPVGEEENGL"
BASE COUNT         1553 a         2045 c         2120 g         1609 t
ORIGIN      
        1 cggcacaact caaatccgca ttcgcgcatc cgaagggttt gatcggtagt ctgagctttc
       61 aaacctaacc ctctcccgca tacaatcatc gcactatgtc tagacctacg cttgttcaac
      121 gctttatcac cgtcggcgcc atatttttac ttggaagtgc cgcgatccag gcccagacaa
      181 cgcggccgag acgcacaaac aggcagcccg cgcaaacgac caaaacccct gccgacgacc
      241 cgctcctcaa acccgaaccg cagccctcgc cgaccgcgcg aagaagcagc tcgaacgatt
      301 cgttgctcga tgtgcagccg gttcgaccag tggcaaatac cgtcgcctcg gcagacacga
      361 agcacgcgtt tctgctgctc gaccagaaac agtttgcggc cgctttgaaa gaagctaagg
      421 agcttacggc ttcatatccc aacgatccgg aagcgtggaa gatcgcaggg tttgcggagt
      481 tgaacctcaa gcagtatgcg gaagcagtga aggatctcga gaaagcagtc gagttgcagc
      541 gcaatgcgaa gcaggacgat cccaacacgg agagcgccct cggacaggcg tacgttcttt
      601 ccgagaagta tcaggaagcg ctgccactcc tgaccgcagc cgtcaatcgt aatccggctc
      661 aacccgattc gatgttgatc tactatcgcg gcatttcaga gtacaagaca ggcaagatcg
      721 cggatgcaga aaagagtttc aaccaagtcc tcaaactcaa tccgaaggat tcgctgtcag
      781 tcttttatct ggcgcaaatc gcactcgcga agaacgatat tgacggagcg atcgcgagtc
      841 tgaatcgcgc caccgttaac gatccgcgca tgacaggtgc gtgggcgctt ctgacaacgg
      901 cttacctgcg tcgggcggcg acgagcaccg atcaggcaaa agccgatgct gactacatca
      961 gcgccgtgcg tgcgggcgag ggtttgatca aactgcgaac tgatgccgaa gcagtcacgc
     1021 ttttcggaca ggctttgatc ggctcgaaac agtatgcccg cgcggcaggc gtgctcgaac
     1081 gcgcgaccgt cgctccggat gcgactggcg tgacgtttta cctgctgggt gtggcccagt
     1141 cgcgcgcgca gaattttccg aaggcgatcg cggccctcga atcagccgcg aagaaaacgc
     1201 ctgacgacgt gaatatctat cgcgagcttg gctatgcgta tgaagtgacg aagcagtacg
     1261 cgaaggcgct gggtgcgtat cagaagggct ccagcttggc gccgtcagat gcagatttga
     1321 aggaatcgat cgaacgcgtc aaaccgttcg cgaaataaga atgagtggct tgcctgggac
     1381 agcaagggcg tttgcgactt attctctggt gccgtatctc ggcatcttgt tctgtcccgg
     1441 tgcggtcgtg ctcggcgctg tcggcgtggt tagatcttat cgttcacagc aagtcgttgg
     1501 cgactcgtct cgcggcatct gttacgcaag cacgattgtt ggagttgtgg taacggcagt
     1561 tcagttgttg ctctggtggg ttctctacca ggcgccaatg tgggcaaatc aactcaatta
     1621 gtctaaggag aatgcaatga aaatcatttc cgctctgctc ctgctgggag ctttgctcac
     1681 gggcgcatat gcgcagcgtc cacgctccat ggatcctcaa ccggcgacca cgccggccac
     1741 cgttgcgcgc aagccagccc cggctccgaa gacggtcaag gcgaaatacg agggaggagt
     1801 gtttggctat aacaagacga tggacggcac gctgacgttt gacgacgcca acaaccgcct
     1861 gatcttcaaa gacaagaagc caccaaaaga gatctcgatt ccgtacgagg caatcacttc
     1921 ggctttcgca gattcaaaaa aggtgcagcc agccgccgcc acggtggccc agaacgtgcc
     1981 gtcgatttat tcactaccgg cgcgctttat caaacacaac gttcgctacc tgaccttgca
     2041 atacagcgat ccggactcga agatttcagg aatcacgagc ttcaagatca gtgacaagga
     2101 actgcttgaa tcagtgctgg tcacgctagc ggaaaagacc aacatgacgc ttcgcgggga
     2161 cgtttacgtt aagaagaaac ccgaagactc cagcaaggcc acaccataat tagccacaaa
     2221 aggcacaaaa agcacaaaag agaaagttca gagttcttta gctctaaacc ttccttttgt
     2281 gctttttgtg ccttttgtgg ctattctttt gacaatccat tccagaccat gcggtggaag
     2341 agttcgtcca tctgctcggg ccgttcgcgc atgccgcggt cgagccacca cgccagcaac
     2401 gacagcagcg cgcccgcgtg tgtgaatgcg attgccgagc gttcgcttgt ggggatggat
     2461 ttgccgcgcg gtaattcgga gaggcgtcgc tctattccgc gtgcgaaatg tccacgcgcg
     2521 agctccatgt tttcctgaaa cttgcccgat ttaacgagcg ctttgtagaa cggttgcacg
     2581 tctgcgaggt ggttgaagaa ttccttcacg ggaaacacgc ggtccgattt gtcgtcgtgt
     2641 tcggagagcg ccatcgagag tgcctcaaaa aactcctccg cgtcgctcat cagcaggtcg
     2701 tccttgtcgc tgtaatgcat gtagaaagtt gaacgactga cgtgcgcacg gtccaacacc
     2761 tcctggaccg tgatcgtttc gaacggtttt tcctgcatca gcgcgatcag cgcatcgccc
     2821 agcgcgtcgc gcgttcgccg cacgcgagca tcagtcttct tgttcgatcc ggcaagaggc
     2881 aggtgctgat tcttggacat tcgttacctc tttgttcgat tacgacccgc gcggccatca
     2941 taccgctgga cagaatgctt ccgatctgaa aaatacacgc gagcaaacta tgatctcctt
     3001 aaaccacgtc tccaaaactt acgaaacacc ggctggcaag ttcgccgcgc tgaaagatat
     3061 cgatctcgag attcgaccgg gcgagtttgt cggcgtcgtc ggcaagtcag gcagtggcaa
     3121 gtcgacgctg cttaacatgg tggcaggcat cgatcgtccg tcgtccggtt cggtcgcagt
     3181 cgcaggcaca gagatccacg cgttgtcaga aaataaactg gcggcgtggc gcgggcgcaa
     3241 tgtcggcttc gtgtttcagt tctttcaact gctgcccacg ctcaccgcgg ctgagaacgt
     3301 tatgctgccg atggacttct cgaaaacgat cccattgcgc gagcggcgca aacgcgcggt
     3361 tgagctgctt gagctggtgg cggtcgggag ccatgctgac aaacttccgg cgacgctttc
     3421 cggcggcgaa cagcagcgcg ttgccatcgc acgcgcactc gcgaacgagc cgccactggt
     3481 tctcgcggac gagcccaccg gcaatcttga ttcgatcacg gcgactgaga ttctcgatct
     3541 gtttcgcaac atggcgaatc gcggtacgac tgtcgtcatc gcaactcacg aagccgacat
     3601 cgcgcgcgta atcgatcgca agatcgagat ctctgacggg aagatccatc cacatatttc
     3661 gcagatgact taaataatga ccacgatttg gaaaaaggct attcgtgact tctggcagga
     3721 acgtgcgcgt acgacgctcg ttgttttggc gatcgcgctc ggcatctcgg cgtttgccgc
     3781 ggtgttgtcg tcatacgcga tcctgacgcg cgagctcgat cgtggttatc tcgaaacgaa
     3841 ccccgcatcg gcggtgctgc gcgttgattc gattgacaaa gatctcgtca aagcgactct
     3901 ccaaaatccc gaagtgagcg acgcagaacc gcgccgcacc atcggcggcc agatcaaggc
     3961 tggtccagtg caatggcgca acctgatcct gtttgtagtt gaagactacg ggaacattcg
     4021 cgtcagcaag ctcgtgccgg agagtggcgc gtggccgcct gcgacgggcg agatactgat
     4081 cgagcgcgat gcgtttcaag tcgcgaaggt aaagatcggc gacgccgtta ccgtcaagac
     4141 tgcgaacggc gtagagacac cgctcgtgat cagcggcagc gttcacgacg taggccaggc
     4201 gcaggcgcgc atggagaaca tcgtctacgg ctacatcaat ctcgcaaccc tcgtccaact
     4261 cggcgaagag cctcgtttcg accagttgaa cattctcgtc gcgcaaaaca ggtttgacga
     4321 ggaccacatc aggcacgttg cgagcgacgt cgagaagttg atcgaagccc acggccacga
     4381 cgtgcgtcgc gtcgagattc cccggcccgg caagcatccg cactcggatc tcacgggcat
     4441 tctgctgttg gcgatgtcga gctttggact gtttgtgctc gtgttgagcg gcattctcgt
     4501 gatcaacctg ctgacggcga tgatggcgtc gcaggtgcga caaatcggcg tgatgaaagc
     4561 gattggaggc acacgctggg cggtagcgcg aatctatttt gcgcaggcgt tgtttcttgg
     4621 cattgcggcg gtgctcgtgt cgataccgct cggaattctc ggcagtcgtg cgctatgccg
     4681 ctacatggcg atatttctca atttcgacat caacagcttt gcggtgccga tctgggttta
     4741 cctgctcgtc gcgttgatcg gcgtgatcgc gcctgtggtc gcggcggctt atccggtttg
     4801 gcgtggcacg aagacaccgg tcaaggtcgc gctctcggat ttcggtttgt cgcaaacaaa
     4861 cttcaagacg ggcgcagtcg atcgtgcggt ggcgcagatt ggcggcccgt ttcgtttgct
     4921 tgcgttcgcg attcgcaaca gctttcgacg acgcgcgcga ttcgtgttga cgctgttgac
     4981 gctcgcggct ggcgggctgt ttttcctttc agcgttgaat gtccgcgcgt cgatggtgaa
     5041 cacgctcgac cgcatgtttg cagcaaggaa atcagatcta tccgtgtcgt ttgcgaagcc
     5101 atacgacttc gagaagattc agaaagcggt gagcaatacg gcgggcgtga agcgtgcaga
     5161 aggttggttc aacggtgagg gatcgcttgg tgaacgccgt ttcggtgtcg ttgctttgcc
     5221 ggctgacaca cagcttctcg acttcgacat catcgaggga agaaagctaa cgccgcaaga
     5281 tacagatgcg atcgttatca ataacgcgct tgctggtcga gagccgcaga tgcacgtggg
     5341 cgaaactgtt gcgttgcgca ttggagcgga ggaaaaaacg tggcgcgtgg ttgggattgc
     5401 gcatgaggcg ttcgcgcctt cacccgtcgc ttacatcccg ctcaccggaa tggttaacag
     5461 tctgcggctc gcgctggagg acactgatcc agatgcagtc ggcgcactga aaacggagct
     5521 cgatcgaaat ctcgaggcgc aaggcgtacg cgcgcgcggc agctcgacga agagcgagag
     5581 ccggttcggg ttcgatcagc atatggtgat gatctacatg tttctgatcg tgatgtcggc
     5641 gattgttggt ggtgttggtg gtctgggact gatgacgacg atgagtctca acgtgctgga
     5701 gagacgacgc gagatgggtg tgatgcgagc gctcggagcg acgccgcgga tcgtgtggct
     5761 aatgatcgtg gcggaaggcg tggtgattgg actgttgagc tggaccatcg cggcgttgct
     5821 cgcgtggccg gtcagcaagg caatcggaga ttctttcgtg agaatgctgt tccacagtgg
     5881 actggatttt actttcgagc cgctgggatt ggtgatctgg atgctcgtgt cgatcttgtt
     5941 gagtgccgtg gcgagttttc tgccggcgtg gaaggcttcg agaactacgg tgcgtgaagc
     6001 cttagcgtat gagtgaccac ggattacacg gattaaaaat ccgatttttt atccgtgtaa
     6061 tctgtgttac ccagtacggt gcaaaatgaa gtccgcgatt ttttgaagaa cgtcagtaga
     6121 tcgatcgagt tgatcgagtg cgtgacaggc atctcgatgt gcgttgatgg ctgcggcgcg
     6181 cgtttcttcc aaaccgtgta gcgctggata cgttgcttta ttcgatcgtg cgtctttacc
     6241 gggcgttttg ccgagatctt ccgcagtggc ggttacgtcg agaaagtcgt cggtaatttg
     6301 aaacaacaat ccgagatgtg atccgtaact ggtgacagcc gtaagctcgc tctcgtctgc
     6361 acgtccgatg atcgcaccgc aacgtgccgc tgctttgatt agggcgccgg ttttgagacg
     6421 gtgaatctcc tcgagttgcg cccgacttac ctgccgcgtt tcggcttcca tgtcgagcgc
     6481 ctgtcctgcc accatgccat ccggcgttgc tgccgctcgg gccagctccg cgatcaaagc
     6541 gatacgcgtc tcagcatcca aagcgacgtc ttcggcgatc gtttggaacg cgagcgtctg
     6601 caacgcatcc ccggcaagaa tcgcggtggc ttcatcgaaa cgcacgtgac acgtcggccg
     6661 cccgcgtcgc agctcgtcat cgtccatcgc cggcagatcg tcgtgaatca gggagtaagt
     6721 atggaccatc tcaaacgcgc acgcggtccg tatcaacgct tcgacaggtg cgctgaacgc
     6781 ctggcccaca gccagcaaca acgcgggccg gaaacgtttc ccacccgcaa acacactcca
     6841 acgtatcgcc tggtgaattt tctccggcgc agtgccagac aatggcagca gcttgttgag
     6901 ctcgagctca accagcgccc gactgctcgc cagcagactg tcgagctcaa gtgggttctg
     6961 gacgacgtta gaggccattc tcctcctcac ccactggttc cttaaacgcc gccgcaaccg
     7021 gccgtccctg gttatcgcgc aagaggatct cgatgcgccg ctcggcctgg ctcagtcgct
     7081 cctggcattc gcgcgagagc cttatgccct gttcaaacaa ctcgagactc ttttcgagcg
     7141 gtaagtcgcc gtcttccaac tgccgcacga tttgttcgag cgcttcgaga gaagtttcaa
     7201 acgtcctgct ttgttctgca ccgttactca tctttatctt ccacgacgcg atctcttcgg
     7261 ccttgccttc ttcatgaatg gaaattccgt gtcgccgctg ccgatcgccg gcagatcttt
     7321 catcaca
//
LOCUS       DASSFQ010000080         5193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_629125, whole genome shotgun sequence.
ACCESSION   DASSFQ010000080 DASSFQ010000000
VERSION     DASSFQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629125"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFH96_02505"
     CDS             complement(<1..465)
                     /locus_tag="VFH96_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6972864.1"
                     /translation="MPRKIPQILTVDDDAVTCDLLCEVFAREGFNAAFANSGEAALEV
                     VAQQPPDLLLSDIRMKTRLDGLSLLEIVRREYPSIPVVLMTAFGSIETAVRAVKEGAF
                     DYISKPFNIDELVAIVRRALTNGTSKQVASELDEDERSSGLIGRTPAMLEIYK"
     gene            complement(455..2029)
                     /locus_tag="VFH96_02510"
     CDS             complement(455..2029)
                     /locus_tag="VFH96_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6972865.1"
                     /translation="MRLRFNLQQKILLLVAGSMSLILLASSYLHSGRIRSLIEKDHYD
                     NAVSQTVALGNRIAQYDYFSNLEDLQQEMQVVVSSRPDFRQIDVYENQPTVPRLIATT
                     ATGTNDLLASWDSIGPSISSPTSSEINRNNGQYWLITTPVTNGQHSGFIKALVLKSYH
                     RNLVSNLHRDYNFLLFGAVIAAVLLLYLLFAYFFRKPVKEIVLTMAHARKGDLSARSP
                     VHRDDELGEIARGFNQMMDDIASRAREREVLLSQIGSLNGELVKKVHLATSELRAANG
                     NLIRTQQRLSHAERLAAIGQVTASLAHEIGTPLNAINGHLQLLARNHPSCGDTQRRLK
                     IINTQLTTIVQSVKSLLERTHRRPIHPQPTDINLIVEELLLLVAPIFESHNIRVSEQL
                     DEGLPFVLADRESLHQVFLNLVNNSLEAMPGGGEMKFVTNYLAGHGLIEVRLIDSGMG
                     ICDEAKKHLFEPMWTTKNSGSGLGLAIAREIIVEHGGKIEYVEVENGAEFRVVLPIVE
                     AKSVAAEFVEVSLDAA"
     gene            complement(2219..3610)
                     /locus_tag="VFH96_02515"
     CDS             complement(2219..3610)
                     /locus_tag="VFH96_02515"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6972866.1"
                     /translation="MKNILVVDDDQDSCDLLREIFCAEGWHVDTALSPSQAFTVAEKE
                     KIDLVVSDVNLEANQSGLDLLKDLRAQCPVILVTGFGTLDSAVEAAREGAWDFISKPF
                     KVQEVVAIARRALEHNRNNPDAERRAEQISAPYEQGGLLGRSPVMIELYKEIARVAPT
                     RSTILIVGESGAGKELVARSIHQHSTRSAGPFVAVNCGALTETLLEAELFGHVRGSFT
                     GAIGDRKGLWEEATGGTLFLDEVGETSPAFQVKLLRALQEGEIRRVGASKATQVDARI
                     LAATNRNLEQDVKAGKFREDLFYRLSVVTLRVPSLKERRTDIPLLAEHFLAIVLDREA
                     HKQLALSEETIRTLVAYNWPGNVRELESAIEYAVLHARGHAIVPEDLPEKLQSAQVRA
                     AARSPLSALFEDLPALDELERRYLLYILEVAGGNRTRAAEILGIDRRTLYRMIERYGI
                     DKATVAILPLDPN"
     gene            complement(3611..>5193)
                     /locus_tag="VFH96_02520"
     CDS             complement(3611..>5193)
                     /locus_tag="VFH96_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6972867.1"
                     /translation="HRGPPLQNVPQRQNHKKLKDKPNGKVASSLPSTSKVRMRTVKLH
                     SKTTLLSSVLIVVMLIAALVVTSAAIANIERTDDQTLAETQARDLAQHISDIGSRDPE
                     AVTRAADLIKGSRPNIISVRIWQVSQGEFTEKTAAAGSAPATPIPEETKNILRRGPGP
                     TVVNQPRTASNQSLYRAFAIIFQNGQPSGAVEIVQHFDSIWTVAVRYLKSVIWMSLIA
                     ITLVMIGTYFLFRQLQLTTKLETEQGILRDLVRDATTELEKRNQQLQETNLELWRSNR
                     RMNELGRLAAAGQTAAHFAHEVGTPLNLISGHVQLLKSDLDRDPRDAESRIRTISAQI
                     ERIERIVRRMLDKTRFETELAPLDLNSVLRKLCDAMSPAFDKRNIHLVENFADNLPLI
                     AGSSDRLQQLFLNLLNNSLDAMPEGGEIQIHTAIESKHGKSQRILVDFIDTGTGMTPE
                     VMSHIFDPLYTTKDRGHGTGLGLVIVSQIVSEHGGKVEVESELGRGTRFRLMFAAIPS
                     DVPALVEDHEPELEVGIH"
BASE COUNT         1104 a         1434 c         1425 g         1230 t
ORIGIN      
        1 cttgtagatt tcgagcatcg ccggcgtgcg tccgattagt cctgaactgc gctcgtcctc
       61 atcgagctcc gaggcgactt gtttgctcgt tccgtttgtg agtgcacgcc gcacaatcgc
      121 aaccagctcg tcgatgttga agggcttgct gatgtagtca aacgcccctt ctttgactgc
      181 gcgcaccgcc gtctcaatcg atccgaaagc cgtcatcaaa accaccggaa tcgacggata
      241 ctcgcggcgc acaatctcca gcaacgacag tccgtcaagc ctggtcttca tgcgaatatc
      301 gctgagcagc agatcaggcg gttgctgcgc gacgacttcg agcgcagcct cgccactatt
      361 cgcgaacgca gcattaaagc cctcgcgcgc gaaaacctcg caaagcaggt cacaagtgac
      421 cgcatcgtcg tcgactgtca ggatctgtgg aatcttacgc ggcatccaaa ctcacctcaa
      481 caaattccgc cgccactgat ttcgcttcca cgatgggtag cacaacgcga aactcggcgc
      541 cattttcaac ttcaacgtat tcgatctttc cgccatgttc gacaatgatc tcgcgcgcga
      601 ttgcgaggcc gagaccactg ccggaatttt ttgtggtcca catcggctcg aacagatgtt
      661 tcttcgcctc gtcgcaaata cccattccgg agtcaatcaa ccgcacctcg atcagtccgt
      721 gcccggcgag atagttagtc acaaacttca tctcaccgcc accgggcatc gcttcgagac
      781 tgttgttcac cagattgaga aaaacctgat gcaaactctc gcggtcggcc agaacgaacg
      841 gcaacccttc gtcaagctgt tctgacacgc gaatgttgtg cgactcaaag atcggtgcga
      901 cgagcagcaa caattcttcg acgatcaagt tgatgtctgt tggttgcgga tggattggac
      961 gccggtgtgt tcgctcgagc aagctcttca ccgattgcac aatcgtagta agttgggtgt
     1021 tgatgatctt cagacgacgt tgcgtgtcgc cgcaactcgg atgatttcgt gccagcagtt
     1081 gcaggtggcc gttgatcgca ttcaagggtg ttccgatctc atgtgcgagt gacgctgtta
     1141 cctggccgat ggctgcgagt cgctcggcat gcgagagtcg ctgttgtgtg cggatcaggt
     1201 ttccgttagc agctcgcaac tcacttgttg cgaggtgaac tttcttcacg agctcaccgt
     1261 tcaagctgcc aatctggctc aacaacactt cgcgctcccg agctctcgac gcaatatcgt
     1321 ccatcatctg attaaagccg cgtgcaatct cacccagctc gtcgtctcgg tgtaccggag
     1381 aacgagctga aagatcgcct ttgcgagcat gagccatcgt gagcacgatt tctttcactg
     1441 gtttgcgaaa gaaatatgca aataacaggt aaagcaggag caccgctgcg atcacagcac
     1501 caaacagtag gaagttgtag tcgcgatgaa gattgctgac gagattgcga tgataactct
     1561 tgagtacgag cgccttgatg aagccgctgt gctggccgtt tgtcacgggc gtcgtgatca
     1621 gccagtactg gccgttgttg cgattgatct cactcgacgt cggggaactg attgatggtc
     1681 cgatggagtc ccacgaggcc agaagatcgt ttgtgccggt tgccgttgtc gcaattagtc
     1741 gcgggaccgt tggctggttc tcgtaaacat ctatctgtcg gaagtcgggt cgtgaactga
     1801 cgaccacttg catttcctgc tgcagatctt cgaggtttga aaaatagtcg tactgcgcga
     1861 tgcggttacc gagcgccacg gtctggctga ccgcattgtc gtaatgatcc ttttcgatca
     1921 gcgatctaat tctgccactg tggaggtacg acgacgcgag caggatcaac gacatcgaac
     1981 ccgccacaag tagcagaatc ttttgctgca gattgaaccg caagcgcatg ccactcgctt
     2041 ggcaaattcg aagccacaaa aaaacacaga cgaaaaaggg atagccacaa aaaggcacaa
     2101 aaagcacaaa atgattattg attgagcggc gtccactaag tttctcgagt tcgggcgcgc
     2161 ttgatgaaca atcctttatg cgctttttgt gcctttttgt ggctgatctt ctttgtggct
     2221 aattagggtc gaggggcaaa atagccacag tggctttatc gatgccgtag cgctcgatca
     2281 tgcgatagag agtgcggcga tcgatgccga ggatttcggc ggcgcgggtg cgattgccgc
     2341 ccgcgacttc gaggatgtag agcaggtaac gtcgttcgag ttcgtcgagc gccggcagat
     2401 cttcgaagag ggccgagagt ggcgaacgcg cggcggcgcg cacctgcgcc gactgaagtt
     2461 tttccggcaa gtcttcggga acgattgcgt gaccgcgtgc gtgcaggacc gcatactcga
     2521 tcgctgactc aagctcgcgc acatttcccg gccagttata agcgacaaga gttctgattg
     2581 tttcttctga cagcgctaac tgcttgtgcg cttcccgatc gagcacgatg gcgagaaagt
     2641 gttcggcgag gagcggaata tctgtgcgtc gttccttgag tgacggtact cgcaacgtaa
     2701 ccacactcaa gcgataaaac aaatcctcgc gaaactttcc ggccttcacg tcctgctcga
     2761 gatttcggtt tgtagccgcc agaatacgcg catcaacttg cgttgctttc gacgcgccga
     2821 cgcgtctgat ttctccctcc tgtaacgcgc gcaacagttt tacctggaac gccggactgg
     2881 tctcaccaac ttcatcgaga aagagtgtcc cacccgtcgc ttcttcccac agacctttgc
     2941 gatcgccgat cgcgcctgta aacgatccac gcacatgacc aaagagctca gcttccaaaa
     3001 gcgtttccgt gagcgcgccg cagtttacgg ccacgaatgg acctgccgaa cgcgtgctgt
     3061 gctgatgaat cgatcgcgcc accaactcct tgccggcgcc agactcaccc acaatcaaga
     3121 tcgttgaacg agtcggcgcg acgcgcgcga tctctttgta aagctcaatc attaccggcg
     3181 aacgtcccag taatccgccc tgttcgtaag gcgccgaaat ctgctctgcg cggcgctcag
     3241 catccggatt atttcgattg tgttcgaggg cgcgtcgtgc gattgccact acttcctgca
     3301 ccttgaaggg cttggagatg aaatcccatg cgccttcgcg cgctgcttcc actgccgaat
     3361 cgagcgtgcc gaatccggtc acgaggatca ccggacactg cgcccgcaag tccttcagca
     3421 gatccagccc cgattgattt gcttcgaggt ttacatcgct gacaaccaga tcgatctttt
     3481 ccttttcagc gactgtgaaa gcctgcgaag ggctgagtgc agtgtcgacg tgccatcctt
     3541 ccgcacagaa gatctcacgc agcaagtcgc atgaatcctg atcgtcatca acaacaagaa
     3601 tgtttttcat ctaatgaata cctacttcca actcaggttc atgatcttca accagtgccg
     3661 gtacgtcgct cggaatcgcc gcgaacatca ggcggaagcg cgtgcctctc cccaactcac
     3721 tctcaacttc gactttgcca ccgtgttcag aaacgatttg actgacgatc acgaggccga
     3781 gccccgtgcc gtgacctcga tctttcgtcg tgtaaagcgg atcgaaaatg tgactcatca
     3841 cttcgggagt cattcccgtt ccggtatcga tgaagtcaac gaggatgcgt tgtgacttgc
     3901 cgtgtttgct ttcgatggcg gtgtgaatct gaatctcgcc gccttcgggc atcgcgtcga
     3961 gactgttgtt aagcagattc aggaataatt gttggagtcg atccgatgaa ccggcgataa
     4021 gcggcagatt atcggcaaaa ttctcgacga gatggatgtt gcgcttgtcg aacgcggggg
     4081 acatcgcgtc acacagtttg cgcaacacgg agttcagatc gagaggcgcc agctcggttt
     4141 cgaagcgcgt tttgtcgagc atgcgacgca caatgcgttc aatgcgttcg atctgcgcgc
     4201 tgatggttcg tatccgcgat tcagcgtcac gtggatcgcg gtccagatct gatttgagca
     4261 attgcacgtg accgctgatc aggttgagcg gcgtaccgac ttcgtgcgcg aagtgagcgg
     4321 cggtttgacc ggccgcagcc aggcgaccga gttcgttcat gcgccggttg ctgcgccaca
     4381 gttcgagatt tgtttcctga agctgttgat tgcgtttctc gagttcggtg gtggcgtcgc
     4441 gaacgagatc gcgcagaatg ccttgctcgg tctcgagctt tgtcgtcagt tgcagctgtc
     4501 gaaacaggaa atacgtgccg atcatgacga gcgtaattgc gatcagcgac atccagatca
     4561 cgctctttag ataacgaacg gccaccgtcc agatgctgtc gaagtgctgc acaatctcga
     4621 cggccccgct tggctggcca ttctgaaaaa taatcgcaaa cgcgcgatag agcgattggt
     4681 ttgaagccgt cctgggctgg ttgacgacgg ttgggccagg accacggcgc aggatgtttt
     4741 ttgtttcttc gggaatagga gtcgcgggag cgcttcctgc tgccgcagtc ttttcagtga
     4801 attcgccctg ggagacttgc caaatgcgaa cgctgatgat gtttggtcgc gagcctttaa
     4861 tcaggtcggc ggcccgagtt acagcttcgg ggtcgcgcga tccgatgtcg ctgatgtgct
     4921 gtgcgagatc gcgtgcctga gtctcggcga gagtctggtc gtcggtacgc tcgatgttgg
     4981 cgatcgccgc gctggtgacg acgagcgccg caatcaacat tacgacaatg agcaccgagc
     5041 ttagcagcgt ggtcttggaa tgaagcttaa ctgttcgcat cctcactttc gacgtacttg
     5101 gcaaggatga tgccaccttc ccgttcggct tatctttcag ctttttgtgg ttttggcgct
     5161 gcggaacgtt ctgtaggggc ggccctctgt ggc
//
LOCUS       DASSFQ010000081         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1076977, whole genome shotgun sequence.
ACCESSION   DASSFQ010000081 DASSFQ010000000
VERSION     DASSFQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076977"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="VFH96_02525"
     CDS             complement(<1..1727)
                     /locus_tag="VFH96_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET6972868.1"
                     /translation="MYILGINAYHGDAAAAIVCDGRLIAAAEEERFNRFKHSAGFPEQ
                     AIRYCLREAGIGVEELDHVGISRDPSAHLHKKILFAATRVATLARSQIKDRLGNAAKV
                     RDLKDEIARVFGVQKKQLRARFHNVEHHRAHLASCFFVSPFERAALLSIDGFGDFIST
                     MWATGEGNSIDVLGQVEYPHSTGIVYTATTQFLGFPHYGDEGKVMGLAPYGRPRFIDE
                     FCELIRTEENGQFRLNLDYFRHHAEGVEMTWDQGSPVIGRVYSDEFARLFGPPRAEGT
                     ALTDREHDIAASLQLRLEEVAFHVLKNLHERTGLTDLGLAGGVAYNSVMNGKILLNTP
                     FKRIFVQPAAGDSGTALGVCYQIYNGVLKRQRGEVMSGACTGPQFSNEEIRAALDHCE
                     MAFESYSDEELTKRAAQDIAAGLVVGWFQGRMEFGPRALGNRSIVVDPRLAEMKDILN
                     DRIKKREPFRPFAPSILEERVGDYFEQTHPAPTMLMVYQIKEERRQEIPAVTHVDGSG
                     RLQTVSRTVNECYYRLISDFNELTGVPLILNTSFNENEPIVCTPQEAIECFRKTKMDV
                     LYLGNFAVRR"
     gene            complement(1752..2507)
                     /locus_tag="VFH96_02530"
     CDS             complement(1752..2507)
                     /locus_tag="VFH96_02530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6972869.1"
                     /translation="MLTSITPLILTYNEAPNIARSLGALGWAQDIVVVDSFSDDNTLE
                     IVHSFPSVRVFQRAFDCHRNQWEFGLKETSIATPWVLALDADYVLSDELVAELAELTA
                     NAQTAGYRANFVYCIKGRKLRSGIYPPVTVLYRRGAATYVQDGHTQRVAVAGAIEDLR
                     APIFHDDRKSFKHWFGSQKRYTELEAQKLRAADPAELALPDRLRRWRIIVPPVMLVYC
                     LIVRGGILDGWAGFYYAFQRAMAEAMLSHRLDG"
     gene            complement(2508..>2966)
                     /locus_tag="VFH96_02535"
     CDS             complement(2508..>2966)
                     /locus_tag="VFH96_02535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6972870.1"
                     /translation="QLAALGYQVTGVDASASGIQIAQRAYPGVRFVHALIDRDLSLGQ
                     FDLAISSDVIEHLYRPSDLLEAAVAQLKPDGQLLLGTPYHGYLKNLVLAATGKMDTHF
                     SALHDGGHIKFFSVNTLSKLIRTHGFDDLGFTFYGRAPWLWKNMICHARK"
BASE COUNT          658 a          880 c          788 g          640 t
ORIGIN      
        1 cgccgcactg caaaattccc gagatacagc acgtccatct tcgtcttccg gaaacactcg
       61 atcgcttcct gcggcgtgca gacgatcggc tcattttcgt tgaacgacgt gttgagaatc
      121 aacggcacac ccgtcaactc attgaaatcc gaaatcaacc ggtagtaaca ctcattaact
      181 gttctcgaga cggtttgcaa acgcccggaa ccatcgacgt gcgtcacggc cggaatctcc
      241 tggcgccgct cttctttgat ctggtagacc atcagcatcg tcggcgccgg atgcgtctgt
      301 tcaaaataat ctccgacacg ctcttccaga atcgaaggcg caaacgggcg aaaaggctcg
      361 cgcttcttga tccggtcgtt gagaatatct ttcatctcgg caagtcgcgg atcgacgacg
      421 atcgatcgat tcccgagcgc gcgtgggcca aactccatgc gtccctgaaa ccagcctaca
      481 accaaacccg cagcgatgtc ttgcgccgcg cgcttcgtta actcctcgtc actataactc
      541 tcaaacgcca tttcgcagtg gtccaacgct gcgcgaatct cttcattcga aaactgcgga
      601 ccagtgcagg cgccgctcat cacctcgcca cgctgacgtt tcagcacgcc attgtagatc
      661 tgataacaaa caccgagcgc ggtgccactg tcgccggccg cgggctgaac gaagatacgc
      721 ttgaacggcg tgttcaggag tatttttccg ttcatcaccg agttataggc aacaccgccc
      781 gcaagcccca gatcggttag accggtgcgc tcgtgaagat ttttcagaac gtggaacgcg
      841 acttcttcga gacgcagttg caaactcgca gcgatatcgt gttcgcgatc ggtaagcgcc
      901 gtgccttccg cgcgtggtgg tccaaacagt cgcgcaaatt catccgagta cacccgtccg
      961 atgacgggcg agccctggtc ccacgtcatc tcgacacctt cagcgtgatg acggaagtaa
     1021 tcgaggttca ggcgaaattg cccgttttct tcggtgcgaa tgagctcgca aaactcgtcg
     1081 atgaagcgtg gccggccata tggcgccagc cccatcacct ttccttcgtc gccgtaatgc
     1141 ggaaagccga gaaactgcgt tgtagcggtg tagacgatgc ccgttgagtg tggatactcg
     1201 acctggccca gcacgtctat agagtttcct tcaccggtgg cccacatggt ggagatgaaa
     1261 tcgccaaaac cgtcgatcga caacagcgcc gcacgttcaa aaggagagac gaagaaacaa
     1321 ctggcgagat gcgcgcgatg gtgttccaca ttgtgaaatc gcgcccgcaa ttgttttttc
     1381 tgcacgccaa acacacgcgc aatctcgtcc ttcagatcgc ggaccttcgc agcgttgccg
     1441 agtcgatctt tgatctgact gcgtgcgagc gtcgccacac gtgtcgcggc gaagaggatc
     1501 ttcttatgca aatgcgccga cggatctctc gagattccaa catggtccag ctcctcgaca
     1561 ccgatccccg cctcgcgcaa acagtaccga atcgcctgct cgggaaaacc cgccgagtgc
     1621 ttgaaacgat taaaccgctc ctcctcagcc gcggcaatca atcgaccatc gcaaacgatc
     1681 gccgccgccg catccccgtg ataagcatta atccctagta tgtacactct gatccgttct
     1741 aatcggtttt tttatccgtc taatctgtgg ctcagcattg cctcagccat cgcgcgctga
     1801 aacgcgtaat agaaacccgc ccatccgtcc agtatcccgc cacgaacgat caggcaatag
     1861 accagcatga ccggcggcac gatgatgcgc cagcgtctta atctatccgg gagagcgagc
     1921 tcggccggat ccgcagcccg cagcttttgc gcttcgagct ctgtgtatct tttctgcgat
     1981 ccgaaccagt gcttgaacga cttccgatcg tcgtggaata tcggcgccct taaatcttca
     2041 atcgcgccgg caaccgcgac ccgctgcgta tgcccgtcct gaacgtatgt tgctgcgccg
     2101 cgccggtaaa gcaccgttac tggcgggtag atcccgctgc gaagttttct gcccttgatg
     2161 cagtagacga agtttgctcg gtagccggcg gtttgcgcat tggctgttag ctccgcgagc
     2221 tcagccacaa gctcatcgga aagaacataa tcagcatcga gcgcgagcac ccacggtgtg
     2281 gcgatgctgg tttctttcag gccaaactcc cactgatttc gatggcaatc gaatgctctt
     2341 tgaaaaacgc gcacactcgg aaacgagtgg acgatctcga gtgtgttgtc gtcactaaaa
     2401 ctgtcaacta caacgatgtc ctgcgcccag cccagcgcgc caagcgagcg cgcgatgttt
     2461 ggcgcttcgt tgtatgtgag tatcagtggc gtgatcgagg tcagcatcta tttgcgtgcg
     2521 tggcagatca tgttcttcca cagccacggc gcgcggccgt agaaggtaaa gccgagatcg
     2581 tcaaaaccgt gggtccggat cagtttcgag agcgtattga cgctgaagaa tttgatgtga
     2641 ccgccgtcgt gcaatgcgga gaaatgcgtg tccattttgc cggtcgccgc gagcacgaga
     2701 ttcttcaggt agccgtgata gggcgtgcct agcagaagtt gtccgtcagg tttcagttgc
     2761 gctactgcgg cttcgagtag atcggaggga cgatagagat gctcgatcac gtcgctggag
     2821 atcgcgagat caaactgtcc gagactgaga tcacgatcga tcaatgcgtg aacaaatcgg
     2881 acgcccggat aggcgcgctg agcgatctga ataccactcg cggaggcatc cactccggtg
     2941 acctgataac cgagtgcagc gagctg
//
LOCUS       DASSFQ010000082         8476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1524105, whole genome shotgun sequence.
ACCESSION   DASSFQ010000082 DASSFQ010000000
VERSION     DASSFQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524105"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1334
                     /locus_tag="VFH96_02540"
     CDS             150..1334
                     /locus_tag="VFH96_02540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET6972871.1"
                     /translation="MSKRRVVITGRGALSALGATTRDLWNGLLANQSGVRRVQRLVDS
                     GITVTSGGEVDAVSPANVDRDHEIANRAIDDALAESRRAAAECGFIWSTGLDTFQLGA
                     TGFVHRSAGRCFSNLASRFVGPRRMIAAACASGTQAVGEAFRLIQNGQVAACVAGGSS
                     VMLTPFYLIGFAGLQAVAVDNGDDPSLACRPFDKKRKGFALADGAGAMVLETLESAGE
                     RGATVFAEVIGFGVSQDAFDLNRPCDDGAGAELCMRRALADAELPVEQIDAVNAHATA
                     TYSGDLAEAAAIRKLFATRWRELPVSGSKGAIGHAMAAAGVLEAIVAAHTCATGIVPP
                     TVNLRHVDEGCELDHVIGAPRDVNAETVLSVSFGMGGQNAAIVIKRSSHKKAQNTQEL
                     HL"
     gene            1362..2093
                     /locus_tag="VFH96_02545"
     CDS             1362..2093
                     /locus_tag="VFH96_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6972872.1"
                     /translation="MDWGLEDRVVLITGATGYLGRALARGFGGAGATLFLTGRNADAL
                     AEIASELTAQGLAADLTVENAATSVVNRVVEEAGRIDTLIINAGTLADGLVAGLTEDD
                     LARVFELNVMAAFRMLRAALKPMILNRSGNIVVVSSTAAQRPGVGQGAYASSKSALEA
                     LVRTTARETAMRGIRINAVAPGLLAGGMSARILDDHMDQARAFIPMRRPGEAEEVVPV
                     VLFLASPLSSYVTGQVWSVDGGVTA"
     gene            2090..2488
                     /locus_tag="VFH96_02550"
     CDS             2090..2488
                     /locus_tag="VFH96_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6972873.1"
                     /translation="MSARDTAAEVATLVRQTLALPASEPITSLQLFFYDLGFTSMDLL
                     DLLFRIEQHFQIEIPEGTIYHLARGDLAEAEFAEDAVLTEAGRQRLMALLYDTPPELF
                     PARIHATTLPRFCTVGAITRLVDHKLSCSS"
     gene            2488..2934
                     /locus_tag="VFH96_02555"
     CDS             2488..2934
                     /locus_tag="VFH96_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972874.1"
                     /translation="MKSISIDAENGRATGVAFVPADHPLFADHFPGKSLLPGSLILEL
                     TAQVAGPLAEEFVQTQLSLERWAMLGMVRDARFLQPVFLPASLEITAVISRSQSSNVS
                     ANVVVTVADERVMQAELVMMMLESSAEWATAIEARNDRLARWKEAS"
     gene            2937..4154
                     /locus_tag="VFH96_02560"
     CDS             2937..4154
                     /locus_tag="VFH96_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972875.1"
                     /translation="MLSAIDAFFVAYQESSGVLMQVGVEVELKGRITRDDIERMLWHV
                     VKRWPPLGQRLRRDWLGLSWEGKPRVSEMLRVAENRDEWRNRPLNPFRETPFQVLWIE
                     EDQKNHLAFRAHHAVVDGEGFFEVCAEALRALAGKKTATDKTDLKNGSIRVVGVRDAL
                     QRVQQLREEARSDKSARIAVRSNAPGPIGIVERDLDRRHLDGWLCASAWMKAIHRWNL
                     LGDQTSTSLISLEVPVSLRRNRDSLTIGNQISPLVLYGDAKQPLEDLAQQLRQQMSRA
                     LRQQTHLALPSLAAPAKFLPWEVFRLIAASPELTGFATSHFAWLEHAQTIHDDVSRAS
                     NGALQLVDQQLYTPVCLHMGAAVAVLAWPERAQLFLTHRLNALSTSDANVLLDLMVQE
                     LGAQHLNRQQVAV"
     gene            4151..5230
                     /locus_tag="VFH96_02565"
     CDS             4151..5230
                     /locus_tag="VFH96_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6972876.1"
                     /translation="MIIIIGAGPAGLAAAETLAKRNIEYVILEKGPAVANALRRVDPE
                     MHLLSPQGLSLMPDMTPEPGTPTYLPFSVLVHELERYQQQHQLLVNFDCTVLGIRKEA
                     SGFSVRYRAGDGEKHELRGSHIINATGIISQPRLPDNFSLEPCRWRHSIDVRAEDLAA
                     ARKLLVVGGGASAAEVLENWLRVRQEDYRAWLSLRSKLVAIPHWIFGIDVHYFSWLPE
                     HIPTSLFGWRAGRLTEPMTGRAVVRAIRKGLIKRAPAVRSYKNGEAEFVNGERIEPDL
                     IVFATGFSYAVEHLKEVVDLDPDGRPLVKNCESTRTPGVFLLGYRFGRTFASPYLRGI
                     ARDAEFVADRINKMRQDLHVNPEKS"
     gene            5251..6207
                     /locus_tag="VFH96_02570"
     CDS             5251..6207
                     /locus_tag="VFH96_02570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET6972877.1"
                     /translation="MKAVHVKGFGDVDQLELVELPDPEPQPGQVRIRVEASGLNYADV
                     MQRAGLYPGGPKPPFISGIEAAGVVESGDGLPRGARVMVVSAGGCHAERLCVSAAACM
                     PIPESMSFVEAAAFPVQYLTAYHALTTVGRAVPGETVLIHAAAGGVGTAAVQIAKLLG
                     LHVVGTASSEEKRARVLELGADDVVSYEEFDQKLGGKSAPPLILEAVGGDVFRRSLAM
                     LPSLGRLVVFGAASRDVQPIDTLKLLFRSQGVFGLHLNAIFERPSLMGESVKRMFEWI
                     GQGKLKMQVGHVMPLAQIREAHELISSRKSYGKIVFADERGS"
     gene            6226..7467
                     /locus_tag="VFH96_02575"
     CDS             6226..7467
                     /locus_tag="VFH96_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6972878.1"
                     /translation="MFYRLTDYLHFARMYRRNQRRQRVWGRLFEEAVRTKSFKLPVAT
                     VVQLIPTEACNLRCPFCNQWGENGYFFDGVRRAQHMDVEGLKNLMRGISPRDSLISVH
                     GGEPFVYKHIDTLLELLSERQFDVIFTTNGTLLKSHLGQLAKIRNLNFLLSIDGDEPA
                     HDKVRGKGTFKAARDSMRTLFEMRREQGLPLPAVIMSSVVCEWTTDVLERVYDVARDF
                     HALAINYNFRWFLTEDVGQAYERHLQHEFGVKSSGAWRGWVSQNHEKHDYSHVAAALS
                     RVLQKKRRFSPPFVFTTPSQLRGADYQTYFTDYLNTFGNESCFMPFYWARIHASGELI
                     FCPGHPDIIAGNVFRDGLMGSFNSDVAIKFRKHMLTNRLPICNRCCGLYMTNPARPFE
                     QKARKRLALPKQVNTHWPLQS"
     gene            7478..>8476
                     /locus_tag="VFH96_02580"
     CDS             7478..>8476
                     /locus_tag="VFH96_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6972879.1"
                     /translation="MKHRYTPRDYLHFARFYWRGQLRMRQWSKDFEASVRGGTYKLPN
                     AAVVQLIPTEACNLRCPFCNQWGDAGYFLAGSRKVESIDEGAITALIRKLSPRYSMIN
                     VHGGEPFAYKQIDGLLEALAERDFDVLITTNGTLMQSHLHAVAGIRNLAFILSIDGDE
                     STHDGVRGRGTYARITESLECLFELRRELGLPLPLVVMSTVVSEWTRDVIERAYTTAK
                     ALGVFMLNYNLRYFMPETAGVAYEKHLQEEFGVSSSGAWRGWIAPSHEQHDYGETVER
                     LRRLLARTRHRVLPPYVVTGPSHMRGKDFDRWFSDYLDTFGNESCFMPFYWARIHAN"
BASE COUNT         1703 a         2164 c         2652 g         1957 t
ORIGIN      
        1 agcccggcga tagttgatgt tgttccgcac gctgcgaatg gacttgcggc gctcttgtcg
       61 tcgattgcta agggactgct gaggtacgtc gtaccaccgg aacacacaga cggtttttca
      121 actgtgaccg taactctgga aatctcgtga tgagtaagcg tcgcgtagtg atcaccggca
      181 ggggcgccct ctcggcgctc ggtgctacca cgcgcgactt atggaacgga ctgctcgcaa
      241 accaatccgg cgtgagacga gtgcagcgcc tcgtcgattc cggaatcact gtcacgagtg
      301 gtggtgaagt cgatgcagta tctccggcaa atgtcgatcg cgatcacgag attgcaaatc
      361 gcgccatcga tgatgcactc gcggaatcga gacgcgccgc ggcggaatgt ggttttatct
      421 ggtccaccgg actcgatact ttccagctcg gagctacagg gtttgtacat cgctcagccg
      481 gacggtgctt ttccaatctg gcgtcgaggt ttgttggtcc aagacgcatg atagcagcag
      541 cgtgtgcgtc gggaacgcag gccgtcggcg aagcttttcg tttgattcag aacggacagg
      601 ttgcagcgtg cgtggccggc ggatcgagtg tgatgctgac gcccttttac ttgatcggtt
      661 ttgcgggact gcaggcagtg gctgtagata acggtgacga tccgtcgttg gcgtgccgtc
      721 ctttcgacaa gaagcgcaaa gggtttgcgt tagccgatgg cgcgggcgcg atggttttgg
      781 agacgcttga gagtgcggga gaacgcggcg caacggtgtt tgcagaagtg attggattcg
      841 gtgtgagcca ggacgcgttc gatctcaatc gaccgtgtga cgatggcgcc ggcgctgagc
      901 tttgcatgcg ccgcgcgctg gctgatgctg agttaccggt cgaacagatc gacgctgtca
      961 atgcccacgc caccgctact tattcaggcg atctcgccga agcggcagcg atcagaaaac
     1021 tgtttgcgac caggtggcga gagcttccgg tgtcgggatc gaaaggcgcg atcggtcacg
     1081 cgatggccgc agccggcgtg ctcgaagcga tcgttgcggc ccacacttgt gcgacgggca
     1141 tcgtgccacc gaccgtgaac ttacgccacg ttgatgaagg ctgtgagctg gaccacgtga
     1201 tcggggcgcc gcgtgacgtg aatgctgaga cagtgttgtc tgtatcgttt ggcatgggtg
     1261 gacaaaacgc cgcaatagtt atcaaacgga gtagccacaa aaaggcacaa aatacacaag
     1321 agcttcattt gtgacttttg tgcctttttg tggctaaacc tatggattgg ggattagagg
     1381 atcgggtcgt tctcattacg ggtgcgacgg gatatctcgg gcgtgcgctg gcgcgagggt
     1441 tcggtggtgc gggcgcaacg ctgttcctta cgggacgaaa tgcagacgcg ttggcggaga
     1501 ttgcgagtga gttgacggca cagggtctcg cggctgatct cacagtcgag aatgctgcga
     1561 cgagtgttgt aaatcgggtg gttgaagaag cgggccgcat cgatacattg attattaacg
     1621 cgggcacact tgctgatggt ctggtggcgg ggctgactga ggacgatctt gcacgagtat
     1681 tcgaactcaa cgtgatggct gcgtttcgca tgttacgagc agcgttgaag ccgatgattt
     1741 tgaatcgtag tggcaacatc gtcgtcgttt cgtccacggc tgcgcaacgt cccggcgtcg
     1801 gacagggtgc gtatgcctca tcgaaatctg ccctcgaagc cctggttcgc accaccgcgc
     1861 gcgagaccgc catgcgcgga atcaggatca acgctgtcgc gccgggatta ctcgccggcg
     1921 gcatgagcgc acgcattctc gacgaccata tggaccaggc gcgcgcgttc attcccatgc
     1981 ggaggcccgg cgaggcagag gaagtcgtgc cggttgtgct cttccttgcg tcgccgctga
     2041 gctcatatgt aaccggacaa gtctggagtg ttgatggagg cgtaacggcg tgagtgcgcg
     2101 cgacacagcc gcggaagtgg ccacgctcgt acgccagacg ctcgcgctgc cggcgagtga
     2161 gccgatcacg tcgctgcaac tgttcttcta cgatctcggt ttcacgtcga tggacctgct
     2221 cgatttgctg tttcgtatcg agcaacactt tcaaatcgag attccggaag gaacgatcta
     2281 tcaccttgcg cgtggcgatc tcgctgaggc tgagtttgcc gaagatgcag tgttgactga
     2341 agccggacgg caacggttga tggcgttgct ttacgacacg ccgccggagc tttttccggc
     2401 gcgcatacac gcgaccacgt tgccgcgctt ttgcacggtg ggcgcaatca cgcgactcgt
     2461 ggatcacaag ctttcatgtt cgagttgatg aaatcgatca gtattgacgc tgagaacggg
     2521 cgcgctaccg gcgtggcatt cgttcctgcg gatcatccgc tgtttgcaga tcactttccc
     2581 ggcaagtctc tgttgcccgg atcgctcatt ctcgaactca cggcgcaggt tgccggacca
     2641 ctagcggaag agtttgtgca gacgcagttg agcctcgagc gatgggccat gctcggaatg
     2701 gttcgtgacg cgcgtttcct gcaaccggtg tttctgccgg cgtcactcga gataactgcc
     2761 gtgatctctc gttcgcagtc atcaaatgtc agcgcaaacg ttgttgtcac agttgctgat
     2821 gaacgtgtga tgcaggcaga gctggtcatg atgatgctcg aatcgtctgc tgagtgggcc
     2881 accgcgatcg aagcgcgcaa cgatcgtctc gcgcgttgga aggaggcgtc gtgacgatgc
     2941 tctccgcgat cgacgctttc ttcgtggcgt accaggagtc gtctggtgtg ctgatgcagg
     3001 ttggcgtcga agtggagctg aaaggacgta tcactcgcga cgatatcgag cggatgttgt
     3061 ggcacgtcgt gaagcgctgg ccgccgctcg ggcagcgact gcggcgtgat tggctcgggt
     3121 tgtcgtggga aggtaaaccg cgagtgagcg agatgctgcg ggtggcggag aatcgtgatg
     3181 agtggcgcaa ccgtccgttg aatccgtttc gtgagacgcc gtttcaggtg ctttggatcg
     3241 aagaagatca aaaaaatcat ttagcgtttc gtgcgcatca tgcggtggtt gatggtgaag
     3301 ggttctttga agtttgtgcg gaagctttgc gggcgctggc gggaaaaaaa acagctacgg
     3361 ataagacgga tcttaaaaac ggatctattc gggttgtagg cgttagagat gcgttgcaga
     3421 gggtgcagca gttgcgggag gaggcacggt cggataagag tgcgcgaatc gcagtacgca
     3481 gcaatgcgcc cgggccgatc gggatcgttg aaagagattt ggaccgaagg catttggatg
     3541 ggtggttgtg tgcgagcgcg tggatgaagg cgattcatcg ttggaatctt ttgggtgatc
     3601 aaacaagtac ttcgcttata tcgcttgagg tgccggtgag tctgcgacgc aatcgcgata
     3661 gcttgactat aggtaatcag atctcgccgc tagtgcttta tggcgacgcg aagcagccgc
     3721 ttgaagatct ggcgcagcaa ctgaggcagc agatgagccg ggcgctgcga caacaaacac
     3781 atctcgctct gccttcgttg gctgctccgg cgaagtttct gccctgggaa gtgtttcgac
     3841 tcatcgctgc gagtccggag ttgacaggct ttgccaccag ccacttcgcc tggctcgaac
     3901 acgcgcaaac gatccacgac gatgtgtcgc gtgcctcaaa cggcgcgctg caactcgtcg
     3961 atcagcagct ttacacaccc gtttgcttgc acatgggcgc ggcggttgcc gtgctcgcat
     4021 ggcccgagcg cgcgcagctt ttcctgaccc atcgtttgaa cgccctctcg acaagcgacg
     4081 cgaatgtcct gttggacctg atggtccagg agttgggtgc gcagcacctg aaccggcaac
     4141 aggtcgccgt atgatcatta ttattggcgc cggtcccgcc ggtctcgcag ccgccgaaac
     4201 gctggcgaag cgaaacatcg agtacgtgat ccttgaaaaa ggcccggctg ttgcgaacgc
     4261 tttgcgacgt gtcgatccgg aaatgcatct gttatcgcca caaggtttgt cgctgatgcc
     4321 cgatatgaca cccgagccgg gcacgccgac ttaccttccg ttcagcgtgc tcgtgcatga
     4381 gctcgagcga taccagcagc aacatcagtt gctggtcaac ttcgattgca cggtgctcgg
     4441 cattcgaaaa gaagcaagcg ggttctcggt tcggtatcga gccggtgacg gtgaaaagca
     4501 tgagttgcgt ggcagtcaca tcatcaatgc gaccggcatc atcagccagc cgcgcctgcc
     4561 ggacaacttt tcgcttgaac cgtgtcgctg gcgacacagc attgacgttc gtgcggaaga
     4621 tcttgcggcg gcgcggaaac ttctcgtggt cggcggcggc gcctcggctg ctgaagtgct
     4681 cgagaactgg ttgcgagtaa gacaagaaga ctatcgtgcg tggttgtcgt tgcgttcgaa
     4741 actcgtcgcg attccacact ggatctttgg catcgatgtt cactacttct cgtggctgcc
     4801 ggagcatatt ccgacgagtt tgttcgggtg gcgtgccggc cggctgactg aaccgatgac
     4861 tggacgagcg gttgttcgtg ccattcgcaa aggattgata aagcgtgcgc cggccgtgcg
     4921 tagttataaa aacggcgaag ctgagtttgt gaatggtgaa cggatcgagc ctgacttgat
     4981 cgtgtttgcc accgggttca gttatgcagt cgaacatctg aaggaagttg tcgatctcga
     5041 tccggatggc aggccgctgg ttaagaactg tgagagcacg cgcacgccgg gagtgttttt
     5101 gcttgggtat cgatttggga gaacgttcgc gtcgccttac ctgcgcggga ttgcacgcga
     5161 tgcggaattt gttgcagaca ggattaacaa gatgagacaa gatttacatg ttaatcctga
     5221 aaaatcttga aaatcttgtg agcaaatgag atgaaagcag ttcatgtaaa gggttttgga
     5281 gatgtggacc agctcgagct ggtcgagttg ccggatccgg agccgcaacc gggacaagta
     5341 cgaatacgcg tcgaagcaag cggcttgaac tacgccgacg tcatgcagcg cgccggtctt
     5401 tatcccggtg gaccaaagcc accgttcatc tccggcatcg aagcggcagg tgtggttgag
     5461 agtggcgacg gactgcctag gggcgcgcgc gtgatggtgg tttctgcagg tggctgtcac
     5521 gcggagcgcc tgtgtgtcag tgctgcagcg tgcatgccta ttccggaatc gatgtctttc
     5581 gtcgaggctg ctgcttttcc ggttcaatac ctcaccgcct atcacgcgct cacgaccgtg
     5641 ggcagggctg ttccgggcga aacggttttg attcatgcag cggcgggtgg cgtcggcacg
     5701 gcggcggtgc agatcgcgaa gctgctgggt ttgcatgtcg ttggcactgc gtcttcggaa
     5761 gagaagcgcg cgcgtgtgct cgagcttgga gctgacgacg tggtcagtta cgaagagttc
     5821 gatcaaaaac tgggcggcaa gagtgcgccg ccgttgatcc tcgaggcagt tggcggcgat
     5881 gtgtttcgtc gcagtctcgc gatgctgccg tcactcgggc gcctcgtcgt tttcggtgct
     5941 gccagcagag acgtgcagcc gatcgacact ttgaagttac tgtttcgatc gcagggcgtg
     6001 tttggattac atctgaatgc gattttcgag cggccgagtt tgatgggtga atcggtgaaa
     6061 cgaatgtttg agtggatcgg gcaagggaag ctgaagatgc aggtcggtca tgttatgccg
     6121 ctcgcgcaga tacgcgaggc gcatgagttg atctcgagtc gaaagagtta cggaaaaatc
     6181 gtgttcgcgg atgagcgcgg atcttaagac gcggatttgg ttgttatgtt ttatcgcctc
     6241 acggattatt tgcattttgc gaggatgtat cgccggaacc agcgacgaca gcgggtttgg
     6301 ggaagactgt tcgaagaggc ggtccgtact aaatcattca aattgccggt tgccaccgtg
     6361 gtccagttga ttccgaccga ggcttgtaat ctgcgttgtc cgttttgtaa tcagtggggc
     6421 gagaatggtt atttcttcga tggcgtgcgc cgcgctcagc acatggacgt ggagggattg
     6481 aagaatctga tgcgcgggat ttcgccacgc gattcactga tcagcgtgca tggtggcgag
     6541 ccgtttgttt ataaacatat cgatacgctc ctcgagttgt tgagtgaacg acaatttgac
     6601 gtcatcttca ctaccaatgg cacgttgctc aagtctcacc tgggccagct cgcaaagata
     6661 aggaatctta actttcttct atcgatcgac ggcgacgagc ccgcgcacga caaagttcgc
     6721 ggtaagggaa ctttcaaagc agcgcgagat tccatgcgca cccttttcga aatgcgtcgc
     6781 gagcaaggtc tgcccttgcc cgccgtgatc atgagctcgg ttgtgtgtga gtggaccact
     6841 gacgtgctgg agcgcgtcta cgatgttgcg cgcgacttcc atgcgctggc catcaattac
     6901 aactttcgct ggtttctcac ggaagatgtg ggccaggcat acgagcgcca tttgcagcat
     6961 gagttcggag tgaagtcgag cggcgcgtgg cgcggctggg tttctcaaaa tcacgagaaa
     7021 cacgattaca gtcacgtcgc tgctgcgctc agtcgcgtgc tgcaaaagaa gcgacgcttc
     7081 tcaccaccgt tcgtctttac aacgcccagc cagttgcgcg gcgcagacta tcaaacgtac
     7141 ttcactgact atctcaacac gttcggcaac gagagctgct tcatgccgtt ttactgggcg
     7201 cgcatacacg ccagcggcga gctgatattt tgtcccggac accctgacat catcgccggt
     7261 aacgtctttc gtgacggact catggggtcg ttcaactccg acgtcgcgat aaagtttcgc
     7321 aaacacatgc tcacgaaccg actgccgatc tgcaatcgct gctgtggact ttacatgact
     7381 aatccggcgc gtccgttcga acagaaagcg cgaaagcgac tcgcgctacc gaagcaagta
     7441 aacacccact ggcccttgca atcgtagtta ggatttaatg aagcatcgct acacgcctcg
     7501 cgactacctg cacttcgcgc gcttttactg gcgtgggcag ctccgcatgc gacagtggag
     7561 caaagacttt gaagcgtctg tgcgcggcgg tacttacaaa ctgcccaacg ctgctgtggt
     7621 ccaactcatt cccaccgaag cctgcaatct gcgttgtccg ttttgtaacc agtggggcga
     7681 tgctggttac tttctcgccg ggtcacgcaa agtggagagt atcgatgagg gcgcgatcac
     7741 ggccctgatt cgcaaactct cgccacgcta ttcgatgatc aacgttcatg gcggcgagcc
     7801 tttcgcgtac aagcagatcg acggtttact cgaagcactg gctgagcgcg atttcgatgt
     7861 gctgatcacg actaacggca cgctcatgca gagtcatctt catgccgtcg ccggtatccg
     7921 gaacctcgca tttattctgt cgatcgatgg cgatgaatcg actcacgatg gggtgcgtgg
     7981 ccgcggtact tatgcgcgaa tcacggagag tctcgaatgc cttttcgagt tgcgccgcga
     8041 gctcggattg ccgctgccgc tcgtggttat gagcaccgtc gtttcagagt ggaccaggga
     8101 cgtgatcgag cgtgcttata cgacggcgaa agctttgggc gtgttcatgc tcaattacaa
     8161 cttgcgttat ttcatgcctg agacggccgg cgtcgcgtat gaaaagcatt tgcaggaaga
     8221 gtttggcgtc agttcatcgg gtgcgtggcg cggttggatc gcgccgagtc acgaacagca
     8281 tgattacggt gaaacagttg aacgtttgcg tcgtttgctc gcgcgaacgc ggcatcgagt
     8341 gttgccgcca tatgttgtta ctggtccgag ccacatgcgt ggtaaggatt tcgatcgctg
     8401 gttctcggat tatctcgaca cgtttggcaa cgagagctgt ttcatgccgt tttactgggc
     8461 caggatccat gcgaac
//
LOCUS       DASSFQ010000083         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1816136, whole genome shotgun sequence.
ACCESSION   DASSFQ010000083 DASSFQ010000000
VERSION     DASSFQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816136"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFH96_02585"
     CDS             <1..1137
                     /locus_tag="VFH96_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6972880.1"
                     /translation="SSSWREEAKALLVVLGYKADVDQALTNENCEIKMLALQSLFQAD
                     QERAITIATEAIKANPQQCPGFPAAAVSMLGSHAGARAIPLLVDIARSNPDQKLRLTA
                     IKRLGEQRSDQVTDELIKIYDVDRTKEIRAQILRAFAESRTSRGIAKVLEIARAGDDL
                     AMRQYAIRYIGELDDSASLDELIRIYDADKTSEIRAQIMRALAERDDARARVKLFEIA
                     RQGDSPEMRLEAIRRLGDHQRIALDELMQLYTSETNQQIKQGLLRAFANSNDPRAQAK
                     LFEIARGNDPVELRGYAIRQLGEKDDEPSVSQLVSMYDSEQNPQMKLALMRAFGDSKQ
                     KVAVRKLMSIARNDPSVEMRKLAVRFLGESKDPEALKFLEDLLK"
     gene            1216..2802
                     /locus_tag="VFH96_02590"
     CDS             1216..2802
                     /locus_tag="VFH96_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6972881.1"
                     /translation="MRYSMLLFLAIIGIACAGGNHSAAVTPVAAAAADDLQTQSPDTT
                     IQGFTTVQGADLMARLEAAQTRARARQTPYWSAYTFDVRPGIAVDPAIREFNGTMNTM
                     GDTVVFIGTTSAGVTVETRSLAIFLLRDPASNQITRMEVYNLERKREYSGYPVYWLGR
                     ANNEESLNYMRALAAASPLDMLSERAVLGIALHDDARVAGMLKGFVTSSPNQRIRSSS
                     VYWLGQVGGETTFLAGLVRSETEDKKIRRSAAHAIGQSRERGTIPTLQGLYESVKDAE
                     IRKSVISAAGNSLDEQPAWSFLLGISKNDADWESRRTAVRQLGDFKRDDAAEELMKIY
                     NNDANVEVKRAALRSLAETKNPRGQARLLEIARTETNPELRKSAIRVMSDRGEAAVED
                     LLKLFDSEQSAEVRRSILQTLSDIKSTRVEDKLFEVAKGNDTIEVKRQAIRLLGERAT
                     KRSFEFLSATAQSSDGNTEVQVQAVRAISERRAEESVPLLIKIARTHPNQLVRKQAIR
                     SLGESGDPRAVDFFREVLTK"
     gene            complement(2882..>3630)
                     /locus_tag="VFH96_02595"
     CDS             complement(2882..>3630)
                     /locus_tag="VFH96_02595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6972882.1"
                     /translation="SFVVYPENKSKATAVIVIHEIFGMSDWVQSLTDQLAEAGYVAIA
                     PDLLSGMGPNGGGTSSLDRNGVGQAIRDLPPDQITADLNAVADYISKVPASNGKVVVT
                     GYCWGGSQSFRFATNNPNIKAAFVFYGSAPASADGTPDKSALGKIAAPVYGFYAGNDA
                     RINATLPKTTEAMTELKKTYDPVTYEGAGHGFMRAGEAPDPAAPQPKGDKAADDKAAE
                     DYQKNLTAWKGNKKARDEAWVRWKKLLAAI"
BASE COUNT          847 a         1011 c         1016 g          756 t
ORIGIN      
        1 agctcgagct ggcgcgagga agcaaaggct ctacttgtcg tactcggtta caaggccgac
       61 gtggaccagg cgctcactaa tgaaaattgc gagatcaaga tgctcgcatt gcagagtctc
      121 ttccaggccg atcaggaaag agccatcact atcgccaccg aagccattaa agcaaatccg
      181 caacaatgtc cgggtttccc ggcggcggca gtatcgatgc tcggctcgca cgccggcgcg
      241 agagcgattc cgcttctcgt tgatattgca cggtccaatc ccgatcaaaa acttcggctc
      301 actgcgatca agcgtttagg tgaacagcgt tcggatcaag tcaccgacga acttatcaag
      361 atctacgacg tcgatcggac caaggaaatc cgagctcaaa tactgcgcgc gtttgctgag
      421 agccgcacct cacgcggaat cgccaaagtg ttggagattg cacgcgcggg tgacgatctc
      481 gccatgcgtc aatacgccat tcgttacatc ggagaactcg atgattctgc gtcgctggac
      541 gaattgattc gtatctacga cgccgacaag accagcgaga ttcgggcgca gattatgcgt
      601 gcactcgccg agcgagacga cgcgcgcgcg cgcgtcaagc ttttcgaaat cgcacgccag
      661 ggcgactcac cggaaatgcg gctcgaagca attcgccgtc tcggtgatca tcagcgcatc
      721 gcgctcgacg agctaatgca gctctacaca agcgaaacaa accagcaaat caaacagggg
      781 ctgttgcgcg cttttgcaaa cagcaacgat ccgcgcgcgc aggcgaagtt gtttgaaata
      841 gcgcgcggca acgatccggt tgaactgcgc ggttatgcga ttcgacaact cggcgagaag
      901 gacgacgagc cgagtgttag tcaattggtg tcgatgtacg actcggagca aaacccgcaa
      961 atgaaattgg cgctaatgcg cgcgtttggc gattcgaaac aaaaagttgc cgtgcgcaaa
     1021 ttgatgtcga tcgctcgcaa cgatccgtca gtggaaatgc gcaaattagc ggtcaggttt
     1081 ctgggcgaaa gcaaagatcc tgaggcgctg aaatttcttg aagaccttct gaaataaggc
     1141 cgatcaatcc atccacagat tacgcggatt ggtttgtttt atctgcgtaa tctgtggata
     1201 cactcggagg attccatgcg ctattcaatg cttctcttcc tcgcgattat cggaatcgct
     1261 tgtgccggtg gaaatcacag cgccgccgtg acaccagtcg cggccgccgc tgccgacgat
     1321 ctgcaaacgc aatcgcccga cacgacgatt cagggtttca ccacggtgca aggcgccgat
     1381 ctgatggctc gtctcgaagc ggcgcagacg cgggcgcgcg cacggcaaac gccttattgg
     1441 tcggcataca cattcgacgt gcggcccggt atcgctgtcg atcccgcgat ccgtgaattt
     1501 aacggcacga tgaatacgat gggagacacc gttgtattta tcggcacgac ctcggcgggt
     1561 gtgacagtag aaacgcgaag tttagcgatc ttcctgctgc gcgatccggc gagtaatcag
     1621 atcacgcgca tggaggttta caacctcgaa cgcaaacgcg aatacagcgg ttatcccgtc
     1681 tactggctcg gccgtgcgaa caacgaggaa agtctcaact acatgcgcgc acttgccgca
     1741 gcctcgccgc tcgacatgtt gagcgaacgt gcggttcttg gcatcgcgtt gcatgacgac
     1801 gctcgagtcg ccggtatgct caagggattc gtcaccagtt cgccaaatca gcgcattcgt
     1861 tcgagctcgg tctactggct aggccaggtc ggcggcgaaa cgacattcct tgcggggctc
     1921 gttcgcagcg aaacggaaga caagaagatc aggcgctcgg cagcccatgc gattggacaa
     1981 agtcgcgagc gtggaacaat accgactctc cagggactct acgagagcgt caaggatgcc
     2041 gagattcgta aaagtgtgat ctcggcagcc ggtaacagcc tcgatgagca accggcgtgg
     2101 tcgtttctcc tcggtatctc gaagaacgat gcagattggg agtcgcgccg gaccgctgtg
     2161 cgtcaactcg gagatttcaa gcgagatgat gcggcggaag agttgatgaa gatttacaac
     2221 aacgacgcga acgtcgaggt gaagcgtgcc gcgttgcgtt cgctagcaga gactaagaac
     2281 ccgcgcggcc aggcgcgtct gctcgagatc gcgaggaccg aaaccaatcc tgaactgcga
     2341 aagtcagcga ttcgcgtaat gagcgatcgc ggtgaagcag cagtcgaaga cctgctaaag
     2401 ctgtttgatt cagagcagtc ggcagaggta agacgatcga ttctgcaaac gctttcggac
     2461 atcaagagta cgcgcgttga agacaagttg tttgaggtcg cgaagggtaa cgacacgatc
     2521 gaggtgaaac ggcaggcgat tcggctgctc ggcgaacgtg ccaccaaacg cagctttgag
     2581 tttctcagtg cgacggcgca gagttcggac ggaaacacag aagtgcaggt tcaagctgtg
     2641 cgtgcaatta gtgagcgacg cgctgaggaa tcagtaccgt tgctgatcaa gatcgcgcgg
     2701 acacatccga atcaactcgt ccgcaaacag gcaattcgtt cgctcggcga atcaggcgat
     2761 ccgcgcgcgg tcgatttctt ccgggaagtg ttaaccaagt agttttgttt cacgcaaagg
     2821 cgcaaagagg gcaaaggaaa ccttcttctt tgccctcttt gcgcctttgc gtgaaatatt
     2881 cttaaatcgc cgccaagagt ttcttccacc gcacccacgc ttcgtcgcgt gccttcttgt
     2941 ttcccttcca cgccgtcaga tttttctgat aatcctcggc tgccttgtcg tcggcggctt
     3001 tgtcaccctt cggttgcggc gctgccgggt cgggcgcttc accagcgcgc atgaagccgt
     3061 gtcccgctcc ttcgtaagtc accggatcgt aagtcttctt caactccgtc atcgcttcgg
     3121 tcgtcttcgg cagcgtggcg ttgatacgcg cgtcgtttcc ggcatagaag ccgtagaccg
     3181 gcgcagcgat cttgccgagc gctgatttgt ccggcgtacc gtcggctgaa gcaggcgccg
     3241 aaccataaaa cacaaacgcg gccttgatgt ttggattgtt ggtggcaaaa cggaacgact
     3301 gactaccacc ccagcaatat ccagtgacca caaccttgcc attcgacgcc ggcaccttcg
     3361 agatgtaatc cgcaacggca ttcaagtcag ctgtgatttg atccggcggc agatcccgaa
     3421 tcgcctggcc cactccgttg cgatcgagcg aactcgtgcc accaccattt ggtcccattc
     3481 ccgaaagcaa atccggcgct atggcaacgt aaccagcttc agcaagttga tcggtcaaac
     3541 tctgtaccca gtcgctcatg ccgaaaattt catggatgac gatcaccgcc gtcgctttgc
     3601 tcttattttc gggatagacc acaaaactat
//
LOCUS       DASSFQ010000084        46659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2120988, whole genome shotgun sequence.
ACCESSION   DASSFQ010000084 DASSFQ010000000
VERSION     DASSFQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 46659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46659
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120988"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFH96_02600"
     CDS             complement(<1..564)
                     /locus_tag="VFH96_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972883.1"
                     /translation="MRALTQKEQEVFRKDLQDKEREIQRYIGVYLSGLVLVTGWIIGP
                     QSRPLLVMALGNNAYNVFAFLIFVVLNVLFINFLIYKSILIHEIAQFIAYLSEPESGF
                     LYWESWRRSPQSASRRVRTTYTVLLAVLPISVSFLIMYGLWKLLHSDSEYLITQYRAF
                     HQLAASGTENYSDNFLTSEQIISVFSRA"
     gene            complement(602..1114)
                     /locus_tag="VFH96_02605"
     CDS             complement(602..1114)
                     /locus_tag="VFH96_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972884.1"
                     /translation="MNLVEIYISARSEIISNHVLMHWFSIIVAAMLLAGVFIVEKHEK
                     TILSVFLPLLSVAWAAAIVRFDFFIHRQAAYLRELEAKMTESGVPYSLWETWKASSRA
                     TPVVVPLADLIASTVIVIPTIYLLFGPAQRFFEGKGWKGKRAFAWSVTILMALLLSSL
                     AAIPKIAAYR"
     gene            1636..1989
                     /locus_tag="VFH96_02610"
     CDS             1636..1989
                     /locus_tag="VFH96_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972885.1"
                     /translation="MPLDQIIWFVKLEVFVFLVGLAAIIAYRLLTGQINTNYLLYGTK
                     KDGTKYFSPERVQLLLFTLGTAMFYLNTVAQNRTSGAIPDVPTQTLALLGGSHAIYLG
                     GKAYMMLFKRNSKGE"
     gene            1989..2639
                     /locus_tag="VFH96_02615"
     CDS             1989..2639
                     /locus_tag="VFH96_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972886.1"
                     /translation="MMLLLLLQTSQSTPQPSPYIEPWVGTMAAWVALGFLGLIGVAIL
                     YNVFTGKIDLSGLISEPNGDASMSRFQLLVFTFVIAASLFLIIASAGPPPAFPKEIPN
                     GILILLGISSSSYLVSKGIQSSSPEGLSGRGPQVTIKGDRSETTFGGPPVQFQANVVG
                     LSDNSVTWAIDPVNFGAIDDTGKYTPPKSGQPTTVTVTATAKEDVSVSDKETLKVS"
     gene            2650..3585
                     /locus_tag="VFH96_02620"
     CDS             2650..3585
                     /locus_tag="VFH96_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972887.1"
                     /translation="MKKKPTAKPPAKGKRTMSGGKAAAAMIPLALATVLPPALERANR
                     GAAPSTAAAPSGAVIFKPGCRLPFDSIKSSGLVIDAKCTADGNGGNDTAKVLENHAKN
                     NFCVEGTPVVITYDDFKGLQKVADDNDLKKGLKTSRDSLINIFPSSGGAKIGEGTLVQ
                     FVTFLLKAHPSNVGKGKGENVNCKLTEQDDNDIHIELTMDRSVDEPDPCDGVTAEMSP
                     HFRPAEWDRLTELDKFDRPVRVTGPMFFDDSHAPCHDGVRPNPKRISVWEIHPVYQFE
                     ICKAKAKDLKACDVKDNSVWVPLDQWFSSGQDEEP"
     gene            complement(3716..4666)
                     /locus_tag="VFH96_02625"
     CDS             complement(3716..4666)
                     /locus_tag="VFH96_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET6972888.1"
                     /translation="MPQIFLSYRRSESKYIAPMLSEKLEQRFGDDSVFLDVDNIPLGV
                     DFREFIGNAVGQCDVLIALIGEEWVRAVDDRGNRRLDDPSDFVRIEIESALKRSIPVI
                     PVLVDGVKMPAKDELPPSIQSLVYRNATELRAGRDLHQHIEGLIDGLASLFSPKNLPP
                     EKAISVKDPVPDSKQAKVSDRTDAPSDLLEEIQNTLAGFTDGSLFVGTIPTKKLKNAI
                     STYATDVASEDVLLLFDNTVFGSAKDGLILTADAVYWHDGEPDRLRYAEIRKVDTLKY
                     SLSASIVLNDKEIEIRISAENDKVAEAIANVIRLLRELSS"
     gene            4910..5296
                     /locus_tag="VFH96_02630"
     CDS             4910..5296
                     /locus_tag="VFH96_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972889.1"
                     /translation="MKIKLALALSVLVACIACSSQPNNKAFKLPSGKEIKITGMVKMD
                     FPNSDPALVLNYLTDIPINDRVALRKEIDEVWSVFRVDVEKAKLKAGVIRATHMEGSG
                     LVKSGAGYGYVFVKRDDGWHCTEDDK"
     gene            5319..5942
                     /locus_tag="VFH96_02635"
     CDS             5319..5942
                     /locus_tag="VFH96_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972890.1"
                     /translation="MTSLEHWQSAWRELGASSASEELYHQLVACYSEPHRKYHTIQHL
                     NECLTYLESARALADHADEVELALWFHDAIYDTSKKDNEQRSAEWARESVLAAGVSSE
                     KADRIYELIMATMHKAVPVGRDAELLVDIDLGILGAGVDRFDAYEIQVREEYSWVPES
                     LYRAARTKVLEQFVSREWVYSTELFRNKYETRARENITRSLARLRID"
     gene            6039..6374
                     /locus_tag="VFH96_02640"
     CDS             6039..6374
                     /locus_tag="VFH96_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972891.1"
                     /translation="MICDMRLVLVVIALALLLVESGGVFRPARTTNAAPANPPTASEP
                     KTRVDFDTQLKPIFQSQCMPCHFSGGKMYDRLPFDKPATIKKLGTRLFTRIKGEHDRK
                     LIEDFLAQD"
     gene            6420..6995
                     /locus_tag="VFH96_02645"
     CDS             6420..6995
                     /locus_tag="VFH96_02645"
                     /inference="COORDINATES: protein motif:HMM:NF022215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HET6972892.1"
                     /translation="MTTTKDRRPLAPIIATALMPLGMIVLLRAEGRMIWCACGHFALW
                     VSDWCSSNTSQQLFDPYSFTHVLHGFLFFWLMALLFKRMPRAWQFLLALLLESAWEVF
                     ENTSFVINKYRTETAALGYQGDTIVNSLGDLACALIGFVIARQLGVRRSLLVFVVIEA
                     VLIFWIHDSLLLQILMLIRPVEAIKLWQMCQ"
     gene            complement(7051..7644)
                     /locus_tag="VFH96_02650"
     CDS             complement(7051..7644)
                     /locus_tag="VFH96_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972893.1"
                     /translation="MRRILLVLSCAAVLVFQPAGAKPQTPEPVKHGGKIETRYDGFNY
                     ETVMRLRKMKVNCDGFKDKFKDACVSIEVMLHCPGTQVNYVKQVTLQVVFENKDWVHF
                     HPPDQRDLTLVTDTETLRIGRMSPVSKDTPGTWETKIEILEAKMPYAVFKKMAMAQSV
                     EIQVGRDAVELREKNIAALKDLNTRVIAPASTTTSSN"
     gene            7806..9254
                     /locus_tag="VFH96_02655"
     CDS             7806..9254
                     /locus_tag="VFH96_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972894.1"
                     /translation="MTASFSDGSLTDLADRLRQANEEFTRHYPGETGRRQPVHTVYGG
                     AHLFKSDSAKRLGSLARRSLDQFAPDFLTFARTIDLPGESELPESFDEATELLAFLNE
                     EPERARKEKKGAWLAHTIYRRVIEKLETEPVEDFRIDFEDGYGNRPDDEEDGHAVAAA
                     AETAEGSKKGSLPPFIGIRIKPFNEELRARSFRTLDIFVSTLVDLNRGRLPENFVITL
                     PKITIPEQVATLATVLARLEKHTDLPEGSLKFEMMIETTQSIINHQGQINLPLLLAAA
                     EGRCVAAHFGTYDYTAGCNITAAHQHMLHPACDFAKHMMQVSFAGTGIWLSDGATNIM
                     PVAPHRFVEGGAPLTQEQIEENREVVHRAWKLHYNHIQHSLTNAFYQGWDLHPAQLPT
                     RYAAVYSFFLQGLEAASERLKNFVEKAAKATLVGDVFDDAATGQGLLNYFLRAVNCGA
                     LTEEEAVQLSGLTLEELREASFVKILKNRQAL"
     gene            9295..9864
                     /locus_tag="VFH96_02660"
     CDS             9295..9864
                     /locus_tag="VFH96_02660"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET6972895.1"
                     /translation="MTTKLQTLTINDLDAMPARDGYHYEIIEGELFVSCSPGLTHQRV
                     LANLITLFVIFLRENPILEYVPSPGLVFDDYSGVIPDLVVFLDRRSDAIITGERLTGP
                     PDLVIEILSPGPANIRRDRIIKRQLYSKHGVPEYWIVDPSHPRVEQYVLHGSSLKLMQ
                     TLQREDILSSSAIPGFSCLVSEIFSRIGK"
     gene            9864..11534
                     /locus_tag="VFH96_02665"
     CDS             9864..11534
                     /locus_tag="VFH96_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972896.1"
                     /translation="MTSLKLIISIALTMALLSTTLIGQKPNGGDPDLARKIARFAPTT
                     LTADTSKLTSKDRQALEKIIAAAKLLDPLFLRQVWSGNDALEKKLQADKSATGQQRLH
                     YFYINDGPWSRLDEKEPFIDGVPREKPLNANYYPDDMSKDEFNSWVQTLSEADKQKAT
                     GFFWLIRRGSDQKLTIVPYSEAYKEYLAPAAALLREAAALTTNATLKNFLNKRADAFG
                     SDDYYESDVAWMDLDSPIDLTFGPYETYEDELFSYKAAFESYVTLRDDAETAKLTKFS
                     GHLQELENNLPIDPKYRNPKLGAASPIRVVNEVFASGEGNSGVQTAAFNLPNDERVVK
                     EKGSKRVMLKNVQDAKFNKTLIPISKVVLDPSEQSALAFDSFFTHILCHELMHGLGPH
                     NITAGGQETTVRKQLKELYSAIEEAKADMTGLWALQYMIDRGIIEKSMERTLYTTYLA
                     SMFRSVRFGITEAHGRGVAMQFNYLTDEGAIKFNEAKGTFSIDHAKIKDGVTKLTRDL
                     LTLEAEGSYDKAKAILDKFAVIRPPMQQALDKLKDVPVDIEPVFPLAK"
     gene            complement(11605..12627)
                     /locus_tag="VFH96_02670"
     CDS             complement(11605..12627)
                     /locus_tag="VFH96_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972897.1"
                     /translation="MSFWQNYMHRRERWYHQRDDNRLVRPFEWGLQYVFDHVNGNDPR
                     ELFRSHTARVLPASENFYALPPITDYRLEGDQLTWTSAVHTPSPENNTARARFFPTKD
                     KNSRKAIVVLPQWNAQPQSHVEACRIFNMIGMSALRLTLPYHLERKPPELERADHLVS
                     SNIGRTIQSMRQAVLDTRAAVQWLKSKGYEQIGILGTSVGSCIAFLAFAHEPDLKAGT
                     FNHVSGYVADVVWHGLSTAHVRAGFADHLTLDELRTYWAPISPLPFMRRVAQMGHRPI
                     RFLVARYDLTFPIDLTHQAIAEARRQQLPLDVVWLPCGHYTTGELPWKAIDAWKIATF
                     FRKQFK"
     gene            complement(12713..14344)
                     /locus_tag="VFH96_02675"
     CDS             complement(12713..14344)
                     /locus_tag="VFH96_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972898.1"
                     /translation="MKKIHALLMMVILCTVSFVPLTAQAQSEKALEHRAEISYPIKFD
                     KTTKPLREMFETGEKPEPARGGKDFEPGKPDKVSNNNTPFTDPLADKSVSAPSALAEI
                     KAATNGTPVDPAARVAPPDTTGDVGPNHYVQWVNLRYAVYNLTRDASNNITAFNLAPG
                     FPKQGNVVWQGFGGRCQSDNDGDPIVQYDQLADRWILTQFAVSATPFTQCVAVSTSPD
                     PTGTYFRYAFSYNRSFNDYPKMGVWPDAYYITYNMFRNGSTFQGNTVCAFERAQMLVG
                     GVARQACASTSTSHHSLEPADLEGTTLPPAGSPNFLLSITTTAVNFWRFAVNWGAGTG
                     TLTGPTAVAGVAAFSRACGGGTCIPQPGTTNRLDSLADRLMYRLSYRNLGTREALVIN
                     HSVTSGTGVGVRWYELTNAVGQTLASAAPVLRQQGTFAPTNDFRWMGSAAMDKTGGIA
                     IGYNISNASTIVPSIRYAYRGPLDPLGTLGNETTILNGSGIQTATLTRWGDYSTLSVD
                     PVDGCTMVFTTQYQPANGNFNWTTYIHSFKLTTCL"
     gene            complement(14579..16117)
                     /locus_tag="VFH96_02680"
     CDS             complement(14579..16117)
                     /locus_tag="VFH96_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6972899.1"
                     /translation="MLISVDPSTGEEIGRVPLLDETEVAAAVARARAAQPAWAKLSYH
                     DRARFILRAREVVLEQVDEIANLVSRETGKPVTEAIAMEIVPTLDLMHYFADNAKQIL
                     KRKRVGLGQYNYMARTSYIVYKPLGVVGIISPWNFPWATPLDEVVMALMAGNAVVVKP
                     SELTPLTALKIADVFKQAQLPDGLLEIVTGDGSTGAALVEAGVNKIMFTGSVNTGKRV
                     AEAAAKHLTPVVLELGGKDPMIVFEDANLENAARAAIWGAFCNSGQACASIERCYVHA
                     SIADKFVELVIKETKRLKQDKPSTGDIDVGAMTNQEQLEIVEDHVSDAIQRGAEVRAG
                     GHRLNESAGWFHEPTVVTGVDHSMKLMRDETFGPVLPIMTFRTDEEAVRLANDSIYGL
                     TASVFTRDLERGKRIAEQIDAGTVMINEVVYTHAVAQTPWGGVKQSGYGRTHGSLGLL
                     ELVSAQHIHVNAMPGLADVWWFPYSKRAGQLFHDFARRFTTGSVLNSLPLLPRILRRL
                     IEPR"
     gene            16110..17531
                     /gene="pepP"
                     /locus_tag="VFH96_02685"
     CDS             16110..17531
                     /gene="pepP"
                     /locus_tag="VFH96_02685"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF008131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HET6972900.1"
                     /translation="MSIAELYRTERGSAGSNEVTIMIGKPPPGATALGSVTMRPQGEF
                     VMIRPQLEEFMRRMAPKSVAIIPGAHDTTRSNDTHYRFRQNSDFFYLTGFEEPDALAV
                     IKDQKYTLFVRPRDPEREIWDGRRAGVEGAKSEFGASESFPIAEFESKLREFLDFTDV
                     LYYRLGDDRDLDNVILKEISNMRAINRKPVHPPQTIIDPATIVHEMRVLKSPEEIEIM
                     QRSADIAAEAHCEAMKAAKPGMQEYQLEALIEQVFRRHGASGPAYTSIVGAGPNATVL
                     HYINNDGELRDGDLLLLDAGAEYKGYASDITRTFPINGRYTKAQREIYDLVLKAQMEC
                     VEMVRPGTTHEQLKQHSIEVLTEGMVELGLLKGKPEELIKEKKYEQFYMHGLGHMIGI
                     DVHDVGRYYFGTESRALEPGVVMTVEPGLYIAPDTKDVPEQYLGIGVRIEDDVLCTNN
                     GPRVLTHKVPKQAEEIEELIERG"
     gene            complement(17551..18549)
                     /locus_tag="VFH96_02690"
     CDS             complement(17551..18549)
                     /locus_tag="VFH96_02690"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HET6972901.1"
                     /translation="MHAITLIPGDGIGPEVASNVVRIIEASGVEVQWETHYAGAQALE
                     KFGETLPKELLDSIVRNKVALKGPITTPVGKGFTSVNVGLRKTLDLYANLRPVRALPN
                     VPCRYPELDLIVVRENTESLYAGLEHIVVPGVVESLKIITEKASTRIARFAFEHARRE
                     SRKKITAVHKANIMKLSDGLFLDCFRRVSVDYPDIEADDKIVDNACMQLVMRPEQFDV
                     MLLENLYGDIVSDLCAGLVGGLGLVPGANIGEKGAVFEAVHGSAPDIAGQGIANPTAL
                     LQSGILMLRYLGEREAADRIETAMLNVFREGKVRTRDIGGTAKTAEFADAIIQKMT"
     gene            18745..19747
                     /gene="thiO"
                     /locus_tag="VFH96_02695"
                     /pseudo
     CDS             18745..19747
                     /gene="thiO"
                     /locus_tag="VFH96_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
     gene            19744..21051
                     /locus_tag="VFH96_02700"
     CDS             19744..21051
                     /locus_tag="VFH96_02700"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6972902.1"
                     /translation="MIKAVFLFVLLTAISTSAQGLAPAVKPDGKPDKRSAQELFEEAN
                     GYLGRRFQEFNRQKLPYDPKLEAQTKKEQKDLAVRNAATLQARGSLKGDDLYYLGMLH
                     HLSADADASLTTMRLLLKGDPDGQKAQAARNVVVLYAVKKDLISEATAAVETYRRHQP
                     QSADDLYRMEFLITDWYLRAKDYGPMTTHAKQMLVAAKKFIETNKDEVVKRDEMLLKS
                     SVLIADSYLKTDQKKLAISALADLRRIAIQLPSANLYKDATVRLMRLDPELNTAQLFD
                     DSLTSKNAVLPDLIANDWIEQQQKKLTDFRGQVVLVDFWATWCGPCRYTLPNLARWQN
                     AYKTKGLVILGVTKYYGRGGGKELTADEELAFLRDFRTRNKLPYGFLVEDGDKNEFNY
                     GVFSIPTSFLIDRKGVVRYISLGASEEEIARLGELIKQLVEEK"
     gene            21131..22372
                     /locus_tag="VFH96_02705"
     CDS             21131..22372
                     /locus_tag="VFH96_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HET6972903.1"
                     /translation="MDDNGITIFRILISFAAGILSFLSPCVLPLVPGYISLMSGVSID
                     NLKEGVSSRRAVIVNSLAFNAGLSVIFLVLGTTAGLVGSAITSNPWVRIIGGLVIIAF
                     GLQLIGLLKIGALYKDTRFFSSEKPRGVLGSAALGMAFAAGWTPCIGPILGGIIGLAA
                     TSGGWRSGLILSAFYSAGLAVPFLLTGLGINQFLGFYKNFRRHLHKVEVVSGVVLILV
                     GLLVMSGQSTLLASSKFMALVPNAEGLLNRWFKTETPPAKQTTATANTNFEMAPDVEF
                     QTLAGKPFRLKELQGQVVLLNFWATYCIPCREEIPALNALQHELQPQGLKIVGASLDD
                     SADGVNAYQQEVAKFEYDVLLGGSDAKVKFQQSVLPTTYLIDRQGRIRQKIIGARDKA
                     AWEAAVKPLLAETPATASAAN"
     gene            22385..23005
                     /locus_tag="VFH96_02710"
     CDS             22385..23005
                     /locus_tag="VFH96_02710"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6972904.1"
                     /translation="MKKLLFAVSILALAISPSVARAQAGHEYSPLTETTVNYKNWKLP
                     NLTAEKSEDLRELMAGKKLVMIVYFAPWCGNWKNEAPIAAKLYEKYKAQGFQVIGISE
                     YASKDDVRNYFGKDGPPYPVVTESETREDKQKTAHYGYRQLTGDKRNWGSPWNIFLEP
                     EKVNAKGDVLIEKAFVVNGELIEDEVDKFIASKVGGASAVIQPCKN"
     gene            23044..24003
                     /locus_tag="VFH96_02715"
     CDS             23044..24003
                     /locus_tag="VFH96_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972905.1"
                     /translation="MKLRILYHGHCFDGVASAATFTRFYKERIHPEAVVNYTGLLHRP
                     GNLFDLGMFDSDENAIVDFKYAASDKLTWWFDHHESAFLTPEDEAHFHADKSGRKFLD
                     PTRKSCTEFIADVAHERFGFNPEPIESLVHWAHIIDGALYESPAQCVELKEPALQLMQ
                     VIEADPDDAFIEQIIRELTTHSLEEVATSAEVQRRFQPILKQHLETLEVIRKKAISAH
                     GVVQFDLVDEGYEGFNKFIPYYLHPEATYSVALTRGSQRTKISVGSNPWAPVPRTHNI
                     AKICERYGGGGHAVVGAVSFKPEEVEEARQAVREIVEELSRKS"
     gene            24106..25506
                     /locus_tag="VFH96_02720"
     CDS             24106..25506
                     /locus_tag="VFH96_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6972906.1"
                     /translation="MNGATLPPGFTETSISGLNNPTAMEIAPDGRIFVCQQGGSLRVI
                     KNGALLSTPFLTLNVDPNGERGLLGIAFDPSFASNNFLYVYYTVPSNPRHNRVSRFTA
                     NGDVVVPGSEQIILELDNLSSATNHNGGAIHFGPDGKLYVAVGENANGSNAQTLSNRL
                     GKVLRINSDGSIPNDNPFFNTAAGDNRSIWAFGLRNPFTFAFQPGTGRMFINDVGEVT
                     WEEINDGIAGSNYGWNLSEGPTSNPQFRSPLFSYLHGFSATTGCAIAGGAFYNPATVQ
                     FPQSFVGKYFFADLCTGWIRLFDPSTGTASDFASNISNPVDLKVGADGSLYYLARGAG
                     AVFRVQFNEPMLISEAGSDSAVALDSVTFVRDPFPLTNIFNFSADQRTRLMLFGMNMA
                     LIAGENSSAVTARAEDAAVNVYPLTVEFVGPLPGADGVTQIVVRLPDNTPAGQTLFVS
                     ATLRGRTTNKVRVRMQ"
     gene            complement(25526..25831)
                     /locus_tag="VFH96_02725"
     CDS             complement(25526..25831)
                     /locus_tag="VFH96_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6972907.1"
                     /translation="MSSSIHNLDTLREAAITLLREVESLASQQEPAPARLGLQEEVQR
                     YESELIREALHRTRGNQRRAAKLLGVKVTTLNCKIKRLGIQAGFLSTDYTDVLNKSA"
     gene            complement(25836..26969)
                     /locus_tag="VFH96_02730"
     CDS             complement(25836..26969)
                     /locus_tag="VFH96_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6972908.1"
                     /translation="MKVLAVILLLCVVVLGQTKSFTPVDGANLKAKIDGAIVAGKANA
                     TNGRFWVGYQFEARPGVAIDYEVVDSAGGVYMSVDGTSVMFDPRHETRELGLFLLYDT
                     TRETFIRAEVYNLRRDREYGGYPVYWAGHIGNEESLNYLKSIVDAPAPEVNRLAERAT
                     FAIALHDDARVDAMLTEMIRRPIAEPMRSRAIYWLGNTPETAAKNTLFTEIVRNNQES
                     TEARNAALSALAMSRSAAVLPLLQNLFETMTTRDLKRRALTGIARNDNNDAAATYLIR
                     VAESEKDMELRKSAIANLGRIAGQKSLGALTSTVDCDADIEIQKQAVIAIGRRPKDEA
                     IPILIRTARNHPKMPVRKLAIQVLGQTGDERAVAFFRELLSKD"
     gene            complement(27005..28585)
                     /locus_tag="VFH96_02735"
     CDS             complement(27005..28585)
                     /locus_tag="VFH96_02735"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6972909.1"
                     /translation="MLKNLTSITATKRVALLFCVLVLMTAGIARGQQGTGSGSGSGSG
                     SGSGSGRSQGTGEATTVFRSARDLITDGDWARAQAKFDEYVSQFPNEKNIDAALYWKA
                     YAEQKLAKYDQCRSTIDRLLQKYPSTNWKDDARTLLAQVPGASAAAYADLVRTVQGQA
                     VIAPPAIEQPLIYSRAITVPQVTVAPATPIAPVEGIAIGVGPADDEDDDNSNDPCEFK
                     IVVLQALFQTDVQRGIMAASEWLKPGSTQTVRCKGAALTLLGRNGGKTVTPVILGVAR
                     NEADLKLRARAISALGATNDDSVVDSLRDFAMSSQENSIVEASLYALSKHTGERAITV
                     LSEFATNSSRTIPQRKVAIASIASRPGDPAVDALFRIYDGDQTVEIRKAVIFGFANRK
                     SERAGTKLFDIARSSDNVELRKAAISAISRRGGDKAIEFLLNLYDIEKNEELKDQIIN
                     SLGYGSGMWAPMVMDSGQGVYTTTARSRMEDQRVIRKMIEIARSPQAPMERRKRAIGW
                     LSRSKDPEVLKFLEELLK"
     gene            complement(28777..29640)
                     /locus_tag="VFH96_02740"
     CDS             complement(28777..29640)
                     /locus_tag="VFH96_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6972910.1"
                     /translation="MKCEVCSELLEEYLDGELAPHDQEQVDMHLSACIECSESFAALT
                     AEQEIFALYDREIEVPPFLWTRIAAHTNTPKRSRLASLFGKTSLASAIAVLLIALAVG
                     AIYLLSKPKTPVPTVATTHSTTPSPDPLPKSVQSAAPKRQKPRSRVKPPIVQTVTDQS
                     DVLSSELAYLDMDEQDTALHIEQSKMLLRSIKNVQISDTDEEIDVTYDKALSRRLLNE
                     NVVLRRDAEMKAKFPTKTLLADLEPFLIDIANLPDRARPQDVREIKERVQKTEIVAAL
                     LDYQDHGTNNR"
     gene            complement(29630..30223)
                     /locus_tag="VFH96_02745"
     CDS             complement(29630..30223)
                     /locus_tag="VFH96_02745"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6972911.1"
                     /translation="MTGAFAGTLMNTSADLVTRVCQGDADAFRLIFERYSRPVISFIY
                     DMVNDRGLAEELTQETFVRAFRAIHTMRAETKLSTWLFGIARNVARESLRARARAKNH
                     VDLADKSVMDLSDDKPVPVDGLLSKELNALVRRSLAALDEDKRLVFTLKVLHQCSYEE
                     IAAITGFSLAKLKTDLHRARAEMRKKISPYAGVGYEV"
     gene            complement(30352..32340)
                     /locus_tag="VFH96_02750"
     CDS             complement(30352..32340)
                     /locus_tag="VFH96_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET6972912.1"
                     /translation="MTERRPDHIKFSGRILFLTDDTSLIRRQLEATGDEAAALETELA
                     SRLSAGVLPLMSNISTDEITPGWVCFYYDETLGQYVYVGMREGAVKKDEVKNGGFAVV
                     VSGLSKGCGSSRETAPYAEKAAGIQLVIAQSIEKIYGQNSQNIGLLTSTDFNLIDRIR
                     RGEEIPLTEFTKGIDPISQDIVTHGGLFNYNKARLNGKVSPPPIETPARPMTIVEKII
                     ARHAFVTAGKIGVEAVKPGDALFALADVRFSHEYVTPMAESLFKQALGPDARVSEPES
                     VFAFRDHLTFLGRVMSPKHREMGLLEKAEGLATTQQSFTTQQQIRLYGESPEGGSEAI
                     CHNAVVEDLALPGQIVIGTDSHTCMAGVLGCFAFGVGSTDMANAWYTKDIRIRVPETV
                     RYVLKGKKRADVAAKDVMLFILASEYMKSSKGIGKVLEFAGEDLQNWAMDERATLTNM
                     AVEAGGTTGIIEPDEVTLDYVVRTRGLDPEQVRKGFVYSDEDAEYAAVFEIDLDAIGP
                     MVATPGDPRNGIPIEELDGEVRIDAAYGGSCTGGKMADMDMYASVLENALANGKRVKE
                     GVHLYIQFGSQKIKQYARQRGYIDLFERAGAELIDPSCGACINAGPGASPNAETVTVS
                     AQNRNFPGRSGPGKLYLASPYVVAASAIAGKIVDPKNI"
     gene            32499..33458
                     /locus_tag="VFH96_02755"
     CDS             32499..33458
                     /locus_tag="VFH96_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972913.1"
                     /translation="MKLGQRLSLAVMLAFSVLTLGARQTVLAQETDDPVKIEIYKRFV
                     DNRADRPAVAYQAARDYLQKYNKDKDQYTDYLNKWVMFYERDERKRNLPNLINEKKFA
                     EAYSTGAKILADEPNYLRAQIDLGYAGYLAATAKNESFNTTALEYARKAISEIESGKT
                     PTEWAPFKGKDDTLAYLNYAVGYLTLKTTPDQSIDALIKAAQYDSDIKKTASTYYFLA
                     VAYESGLYKPLSTDYQAKYANKPETPESKAALEKLNVVMDRIIDAYARAIATAGTDPK
                     TEQGRKDWLAAMTNYYKFRHGGTDTGMTQFIASVLQTPLPPKP"
     gene            33460..33972
                     /locus_tag="VFH96_02760"
     CDS             33460..33972
                     /locus_tag="VFH96_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6972914.1"
                     /translation="MSRIKWTDRRFSFDFPAGIYPEMIERIRGTPARLEDLFTGLPAE
                     TLTAQFDGRWSMQENAGHLLDLESLVSQRIDEYLAGNSALHAADMSNRKTYDANHNEV
                     AADSILRAFRAARHEIVNRLESLDAEVFERSALHPRLNVPMRLVDMLFFQAEHDDYHL
                     ARISEIKKAQ"
     gene            33988..35316
                     /locus_tag="VFH96_02765"
     CDS             33988..35316
                     /locus_tag="VFH96_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6972915.1"
                     /translation="MSRIVLVIAAVVLLAPTINAQQLDAMIDRDIASWLTTYRNLHAA
                     PELSHREEKTSAFVASELRKLGFTVTERIGKYENSQWTGFGVVAVMKNGPGPTVLVRT
                     ELDALPVEEKTGLPYSSSVKTKNDAGVDVSVMHACGHDIHMTSFLGTAKMLSELKSRW
                     SGTLVMLGQPAEETGDGANAMLRDNLYTNFPKPDFAIALHDKPELETGKVGYTPGYAM
                     ASATSIDVKIRGVGGHGSAPETTKDPIVVAAQVVMALQTIVSRENSPLDPAVVTVGSI
                     HGGTRYNIIPDEVNLQLTVRTYKEEVRKRVLASIERIVKGVAATAGIPEDRAPIVKVS
                     EGTGSTYNDPKLIERLVGTFKQALGDENVVQMPPMMASEDFGYFSLDHSIPATIIWLG
                     ASDPAKVKASRESGVALPGLHSALFAPVPEPTLRTGVKATTSAVLELMKK"
     gene            35413..36714
                     /locus_tag="VFH96_02770"
     CDS             35413..36714
                     /locus_tag="VFH96_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972916.1"
                     /translation="MNSTLRFLSLLLLLLVFTQTSRATEPTEVLARKAVSENAAASSA
                     AIEELRSLGPAGLQALMTQYAGAINTHIKNPSVAPDEEWQRISAALDAVAQQRNSYIA
                     GLYWYTDLDSAKKAAKALGKPILSLRLLGKLTDEFSCANSRFFRTVLYPNEEVSSVLH
                     DRFVLHWQSVRPVPTVTIDFGDGRKLERTLTGNSIHYILDSDARPLDALPGLYGPKAF
                     VRGLIDTEKLFKSLAGKDDAQRNLTLRIYYEEQHNKISAAWLSDTTKIGGKAPKGFMI
                     VKGRNGDALSIAPLAVTKAITETSILRAMNFATDELGKITDEAAWKKIAQLHPTDAEL
                     DDRSISLIRRQNPTITEDDLTHLTRKFQELVALDTVRNEYLMHTKLYVWLLNDPVRND
                     VEKLNTKVYDSLFLTPGSDPWLGLFSPEVYTALDNGGVKKN"
     gene            36804..38258
                     /locus_tag="VFH96_02775"
     CDS             36804..38258
                     /locus_tag="VFH96_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6972917.1"
                     /translation="MLRSLLLLTLLPISVFTQTQDKVREYHRANERQILKEFTTLLAI
                     PNVASDTQNIRRNAALIVEMMKQRGLNPRLLEGPTPNTPPAVYGEWNTPGAQRTILVY
                     AHYDGQPTDPKQWTETAPWEPVFRSGRIYARSASDDKAGVMAILTAFSALKDKGVALT
                     SNIKFFFEGEEEAGSPHLGEIIDRNKELLAADAWIIGDGPVHQSGRKQVVFGARGDTN
                     VDVTVYAAKRPLHSGHYGNWSPNPANLLARLLASMKDDDGRVTIEGWYSDAEPLGEAE
                     RRAIAEAPQYDEELKGQLGIKRTEGGGKSLLELINLPSLNINGFASGDVGALARNVIP
                     TTATAVLDLRLVKGNDYQRQVQRLVDHIRKRGYHVTDRDPTDAERAQYPLLARVNVRP
                     GGYNAERTRMDLPISKAVIDAVQSTSAEKIVLLSTSGGSLPLSIITERLKTVTISVPI
                     ANYDNNQHAENENIRLQNFWDGIEIWAAIMTMKN"
     gene            complement(38350..38694)
                     /locus_tag="VFH96_02780"
     CDS             complement(38350..38694)
                     /locus_tag="VFH96_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972918.1"
                     /translation="MSKFFLLIACFLIGLLVLACGAPTNRNAQAPSTVSEGEKVGIAE
                     CDNFIAAYETCVNSKVEEGARANVRASVARLRTDWKKMADDPQMRATLAAHCKAQRET
                     TMAAMKNYNCAW"
     gene            38784..40907
                     /locus_tag="VFH96_02785"
     CDS             38784..40907
                     /locus_tag="VFH96_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6972919.1"
                     /translation="MKNKAFPALLLSFCVLLPALAQTKPTAPQQPQKPADDQDEVVKI
                     TTNLVQVDAVVTKDGKLVTDLTADDFEIYEDGKKQAITSFAYISNVPGAALTATKEKP
                     GNGPQPPRPINPNDPHRTIAFVVDDLGISAESMSQVRKQLRKFVDEQLQPNDLVAIIR
                     TGGEMGALQQFTNDKRLLSRAADQLRWNMCNRVGITALPVLGSPSSQGLCGRYSMIST
                     INALRFIIDAMGYLPGRKSMVFMSDHLPVEDQDDLEFLRDIRSGGNDGVKDNANKAAA
                     ARASDEAADSDLGFNDARTYYAALRKIAEKAIRSSVVIYSVDTQGLLTTSLSAADRFY
                     GDGPQFKDVLRQRNFLIWNRREGSDLIAKQTGGFQIRNSNSFELDRIVQDQSGYYLLG
                     YRPTEETFNRKFHHIKAKVKRSGMSLRTRLGFYGVSEDESSKTRLTRRDVTNLALASP
                     FAAQDVEIHLASFFFNDKTYGSLIRSFLYITASDLEFTTVNGRHQASVEVHGVVFGDN
                     GAIVEQLRRGLTISLPENEYVAALKDGLGFNVDIPVKRSGAFQLRVAIRDRETQKIGS
                     SGEFVTVPDLKNKKPAVSGIVLGGGSRGRSEQEALVNTVARRFDLNTDLDFVYAIYNA
                     LQFAKPVMETKLFRDGKVVYSAPEVPIQTAGQPDPERMLVNGKVPLSRDLEPGFYYLQ
                     VVITDKDAKSKALPLVQWIDFEVVK"
     gene            40928..41818
                     /locus_tag="VFH96_02790"
     CDS             40928..41818
                     /locus_tag="VFH96_02790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6972920.1"
                     /translation="MRVTISLLVLMCLCSMTACAQDMNGNHNHGAPAAASPASTDNMV
                     SQEWARQRVEKSPRHREWVKVKNGTREVNSFVVYPENKNKATAVIVIHEIFGMSDWVQ
                     SLTDQLAEAGYVAIAPDLLSGMGPNGGGTSSLSGNAVGQAIQALPPDQITGDLNAVAD
                     YISKVPASNGKIVVTGYCWGGGQSFRYATNNPNIKAAFVFYGPAPASADGTPDKAALA
                     KIKAPVYGFYAGNDARINATLPKTIEAMTELKKNYEPVTYEGAGHGFMRAGEAPDANE
                     ANKKARDDAWARWKKLLAAI"
     gene            complement(41879..43459)
                     /locus_tag="VFH96_02795"
     CDS             complement(41879..43459)
                     /locus_tag="VFH96_02795"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6972921.1"
                     /translation="MRHSMLLFLAIIGIACAGGNRAGTVTVAAASDDLQTQSSSTTIQ
                     SFTPVDGADLMARLEAAQSRARGQQTPYWSAYTFDVRPGVAVDPSVRDFNGSIHTMGE
                     TTVFIGTNAAGVTIETRNLAIFLLRDPSSNQITRMEIYNLERKREYSGYPVYWLGRAN
                     NEESLNYLRAIAAATPLDMLSERAVLGIALHDDARVGGMLKTFISSSPNQRIRSTSVY
                     WLGQVGGEQAFLASIVRNDSEEKKMRTAAAHAIGQSRDRGTVATLQGLYESVKEPEIR
                     RSVISAVGNSIDEQPAFTFLLNVAKNDPDPESRRTAVRKLGNFQREDAVEELMKIYQN
                     DANIEVKRAALRSLSETKSPRAQTRLNEIARNDANPELRKTAIRVLSDRGEAAVDDLL
                     KLYDSEQAPEVKRTVLQTLSDIKSPRVEDKLLAVASGNDAMDVRRQAIRLLGERVSKR
                     SFEFLSQTAQSTDGNAEVQVQAVRAISERKAEESVPLLIKIARTHPNQLVRKQAIRSL
                     GESGDPRAVDFFREVLSK"
     gene            complement(43571..45079)
                     /locus_tag="VFH96_02800"
     CDS             complement(43571..45079)
                     /locus_tag="VFH96_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6972922.1"
                     /translation="MKSLVNYRPQIRSGLALLAVAAVVTVFSIYTQRTSAFDQELSKL
                     NKFVQTKANTASMQIFREGRDFIEAQNWQRAAEKFNDFITGYPKDKDLDAALYWYAYA
                     LQKQGRKDEAATVLVKLINKFPGSSWRQEAQAMLVVLGRKDAVDAALDRDDCEIKILA
                     LQSLFQADEERAIGIATEALRANPAKCQNFQAAVLSILGSHGGPRVTPILLEIARSNP
                     DPKLRLVAIRRLGELKTDQVTDELIKIYEADQTKEVRAQILRALVEGNTPRGTAKVME
                     IARTGTDISARQYAIRYIAEMDDAASLDTLIQIYDADKTKEIRSQVIRALAERDDPKA
                     RAKLFEIARQAEMPELRIEAIRRLGDNNRVTTDELLQLYTSETNVQIKMGLLRTFGDS
                     TDPRAVTKLFDIARGNEPVELRMYAVRQLGEKDDEQTVNQLVSLYDAEQNMQVRMALL
                     RGFGDSKQKVAVRKLMAIAKSDPSVELRKAAVRYLGESKDPEALKFLEELLK"
     gene            complement(45164..46066)
                     /locus_tag="VFH96_02805"
     CDS             complement(45164..46066)
                     /locus_tag="VFH96_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6972923.1"
                     /translation="MNCSFTEKISSLIDGELSPAEAREVERHLVGCNECQEVRADFLN
                     LRSQLAGFETSLEPAVQNRALKKILATPRRAPARGFTWGFGAQAAAFATLVIVAAIIG
                     FLIYRSSNTPTNDKHLAVQTPTPVPSASVQPEASPSPNKGDEQTAPRRSPSPAPKKPL
                     VREPKPGEQFAAIPSTPEPVRSADTQTMTALHFEKSETLLVAFRNVRLNEPGTTAEVA
                     YERKRAQQLVLQNMMLRREADAAGDVQVSSLLESLEPILIDISNLPDKPDKDAIRVIR
                     QRVERKNIVPLLRVNSTALARALD"
     gene            complement(46084..>46659)
                     /locus_tag="VFH96_02810"
     CDS             complement(46084..>46659)
                     /locus_tag="VFH96_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6972924.1"
                     /translation="NSRVLGDVAQLMASEDEEFTMLFQDFYPSLCRFLECLLGGRAPL
                     AQDLAQESFLQLHRTAWGTMPVGEARFWLYRVARNMAINEFRKSHTRYRLFDRVVETM
                     RPRARNPEQEYETQERQTLVLEMLQTLPEDQRTALLLREQEQLSYREIGQVLNVSESK
                     VKVDIFRARASLRERWTRISHVNPVNHVNPV"
BASE COUNT        10531 a        12697 c        12881 g        10550 t
ORIGIN      
        1 ggctcttgag aatactgaaa taatttgttc agaggttaga aaattatctg aataattttc
       61 tgtgccgctg gcggccaatt ggtgaaaagc acggtactgt gtgatcagat attcagaatc
      121 gctgtgaagc aatttccaga gaccgtacat tatgagaaag gagacactga taggtaacac
      181 tgcgagcagt acggtgtagg ttgttcgcac gcgcctggac gcactttgtg gacttcgccg
      241 ccagctttcc caatataaaa acccactttc gggctcactt agataagcga tgaactgtgc
      301 gatctcgtga atcagaatgc tcttataaat aaggaagtta atgaacagaa catttagtac
      361 tacaaaaatg aggaaggcaa agacgttata tgcgttgttg ccgagagcca tcactaatag
      421 aggtctggat tgaggaccta tgatccatcc tgtaactagc acgagacctg ataaataaac
      481 gccaatatat ctttgaatct cgcgttcctt gtcctgaagg tctttgcgaa aaacttcttg
      541 ctctttctga gtgagggctc tcatgaagtt gatcctaaac tctagaacgt aaaaaaatgg
      601 cctatcgata agctgcaatc ttcggaatgg ctgctaacga tgagagcaat agagccatca
      661 atatggtgac gctccaagca aacgcccgtt tacccttcca gcctttccct tcgaagaagc
      721 gctgtgctgg tccaaacaga agataaattg taggtatgac gattacagta gatgctatta
      781 aatcagccag aggaacaacg acaggtgtcg ctcgcgaaga tgccttccaa gtctcccata
      841 atgaataagg cacgccactt tctgtcattt tggcttcaag ttctcgcaaa taggctgctt
      901 gtctgtgaat gaaaaaatca aaccgtacaa tagctgctgc ccacgccacg gagagtaagg
      961 gaaggaacac gctaaggatc gttttctcat gcttctctac gataaagacg ccggccagta
     1021 gcatagccgc gactattatg ctaaaccaat gcatcagcac gtggttgctg atgatttcag
     1081 atctagcaga aatatagatc tcaacgagat tcaaccgaaa cctttaacta aaatcttttc
     1141 aattgacgag gagcctaagt ccctttaggt ctaatgtcaa cggagcattt tgctcgaatg
     1201 ctttcccgca cttcgtgaag gttacggcgc gcagttgagt cgcctgaccg tgaggaactt
     1261 acgccaactc aaggtagaat tcgctttttc aaaggacgaa aatgccctta gttgctaaac
     1321 cgcctcccgc aactgttgca agaaactgag cctttctgcc ggtggcgaca ggaaaaagtt
     1381 gcgaagattg tcccgtatgg gctaccaaca ccttcccctc taacaaatcg ccgcgtggga
     1441 ccggaactaa ggtctgggtg ttacaaccca ctatttagtt gcaaaaactt tagtcctgtc
     1501 agattttgca tgtcagattt gcattgacac gaattctaaa cgagagtacc tttcgcaccg
     1561 cctagcaatt ccgtttttgg cctttcacct cgcgcgagca gaccaccaat tagttacaac
     1621 ccggggattt cgacgatgcc tttggaccag atcatttggt ttgtgaaatt ggaagtgttt
     1681 gtgtttcttg tcggcttggc cgccatcatt gcctacagac tcctgactgg ccaaatcaac
     1741 accaattatt tgctctacgg cacaaaaaaa gacggcacaa aatacttcag tcccgaacga
     1801 gttcaactgt tgctgttcac actcggcaca gcgatgtttt acctgaacac tgtggcgcaa
     1861 aatcgcacct ccggtgccat accggatgtg cccactcaaa cattggcctt gcttggcggt
     1921 agtcacgcaa tctatttggg cggcaaggca tacatgatgc tgttcaagag aaacagcaaa
     1981 ggagaataat gatgctacta ctacttctgc agacctcaca aagcaccccc cagccgagcc
     2041 cctatattga accgtgggtc ggtaccatgg cggcgtgggt cgccttgggc ttcctgggac
     2101 tgattggggt cgcgatcctt tacaatgtat ttacaggaaa aattgatctt agtggcctca
     2161 tcagtgaacc gaacggcgat gccagcatgt ctcgctttca gcttctcgtg tttactttcg
     2221 tgattgccgc gagcttgttt ctaattattg cctccgctgg tcccccgccg gccttcccga
     2281 aagagattcc aaatggaata ttgatactcc tcggcatcag ctcaagctcc tacctggtca
     2341 gcaaaggtat tcagagtagt tcaccggagg gactttccgg tcgagggcca caagtcacga
     2401 tcaagggtga tcggagtgag acaactttcg gcggcccgcc tgttcaattc caagctaacg
     2461 ttgttggatt atcggataac tccgtgacgt gggcgatcga cccggttaat ttcggagcaa
     2521 tcgatgacac cgggaagtac accccaccga aatccggaca acccactacc gtaactgtca
     2581 cggctactgc gaaggaagat gtaagcgtgt cagataaaga aaccctcaaa gtgagctaga
     2641 ggaggcaaga tgaaaaagaa acccaccgca aagccccccg ccaaaggcaa acgaacaatg
     2701 agcggcggca aggctgcggc ggcgatgatt ccgctcgcgc tcgcaactgt tctgccgccg
     2761 gctttggaac gcgccaacag gggcgcagct ccttccactg cagcagcacc ttccggcgcc
     2821 gtgattttca aacccggctg cagacttcca ttcgacagta ttaagtcaag tgggcttgtg
     2881 attgacgcaa agtgcactgc ggacggtaac ggaggaaatg atacggccaa agtgctcgag
     2941 aaccatgcca agaacaattt ctgcgtcgaa ggaacgccgg tcgtgattac ttacgacgac
     3001 tttaagggat tgcaaaaggt ggcagacgat aatgacctca agaaaggttt aaagacaagc
     3061 cgcgatagtc tcattaatat ctttccctcc tccggcgggg ccaaaatcgg cgaaggcaca
     3121 ttggtccagt ttgttacctt tctcctgaag gctcatccct caaacgtggg gaaggggaaa
     3181 ggtgagaacg tcaattgcaa gttgactgaa caagatgaca acgacatcca catagaattg
     3241 accatggatc gatctgtcga cgaacccgat ccttgcgatg gcgtcacagc tgaaatgtct
     3301 cctcatttcc gtccggcaga atgggacagg ctaacggaat tggacaaatt tgatcgcccg
     3361 gtgcgagtga cgggaccgat gttttttgat gatagccacg ccccctgtca tgacggtgtg
     3421 cgtccaaatc cgaagagaat ctccgtctgg gagattcatc cggtttatca attcgagatc
     3481 tgcaaagcaa aagcgaagga tttgaaagca tgcgacgtca aagataattc agtctgggtt
     3541 ccgctggacc agtggtttag tagcggccag gacgaggagc cataaaaccc gcacccctgt
     3601 ggcttagatt gccaccggtg tcaatttgtt gaacgaaact gagcatttct gtcgttggcg
     3661 tcctaaacaa gttgcgaaga ttgtccactg ggggctgcca acttcgttca ataaactacg
     3721 agctaagctc acgaaggagg cggattacat tcgctatggc ttcagctacc ttatcgttct
     3781 cggcggatat tcttatttcg atttctttgt cgttgagtac aatcgaagcc gagagggagt
     3841 atttgagagt gtccaccttc cggatttccg cataacgaag tcgatctggt tcgccgtcgt
     3901 gccaatatac cgcgtccgcc gttaaaatga gaccatcttt cgcgcttccg aaaactgtat
     3961 tgtcaaagag caggaggaca tcttccgacg caacgtcagt ggcatatgtg ctaattgcgt
     4021 tcttcagctt tttagttgga atcgttccga caaacagaga accatcggtg aatcccgcaa
     4081 gagtgttttg aatttcttca agtaagtctg acggagcgtc ggtcctatcc gaaaccttag
     4141 cttgtttgct gtcgggaaca ggatctttta ctgagatcgc tttctctggc ggaagattct
     4201 taggactaaa gagtgatgcc agaccatcga taagcccctc gatatgttgg tgtaaatctc
     4261 gccccgcacg cagttcggta gcattccgat agacaagact ttgaatagac ggcggcagct
     4321 catccttcgc cggcattttc acaccatcga ccagaactgg aatgaccgga atactgcgct
     4381 tgagtgcgga ttcaatctcg attcgtacaa aatccgacgg atcatcaagg cgcctgttgc
     4441 ctcgatcgtc aactgcccga acccattctt ctccaatgag tgcaatcagc acatcgcatt
     4501 gcccaacagc atttccgatg aattcccgaa aatcaactcc aagcggaata ttatccacgt
     4561 ctaagaaaac agagtcgtct ccaaagcgct gttccagctt ctcgctcagc ataggggcga
     4621 tgtatttact ctcactgcga cggtaggaaa ggaatatttg tggcataaat ctaagctgtc
     4681 ctctttgtta actgaagtat gttgcttggc gctaaccagt gggagccgtg tatcgccact
     4741 tcgggtagcg agaaactgct tgagattcag agccagatat taatccagcg tcaagcaaaa
     4801 attccaggtt ttcggcgacc cttccttgaa aaaaataact ccggcaccct tttcttttgc
     4861 taagctgaat tggggttttt actcatagtc cgccatcctc ggagaacaaa tgaaaatcaa
     4921 actcgctctc gcactatctg tgcttgttgc ctgcatcgcc tgcagcagcc agccgaataa
     4981 caaagctttc aaactgccat cgggcaagga aatcaaaatt accggaatgg tgaagatgga
     5041 ttttcccaac agcgatccgg ccctggtctt gaactatctg actgacattc ccatcaacga
     5101 tagggtagcg cttcgcaaag aaattgatga agtttggagt gtgtttcgag tagacgttga
     5161 gaaagcgaaa ctgaaagccg gtgtcatccg tgccactcac atggagggct ctggcttagt
     5221 caaatccggg gccggttacg gttacgtttt cgtcaaacgc gacgacggct ggcattgcac
     5281 tgaagacgat aaatagattc agcagtgagc ttatgactgt gacttcactg gaacattggc
     5341 agtctgcgtg gcgagagctt ggtgcgtcga gtgcaagcga ggagctttat catcaactcg
     5401 tcgcgtgtta ttcagagccg caccgtaagt accacacgat ccagcatctc aacgagtgcc
     5461 tgacgtattt ggaaagtgcg cgcgcactgg cggaccacgc ggatgaagtt gagctggcgc
     5521 tctggtttca cgacgcgatc tacgacacca gcaaaaaaga caacgagcag cgcagtgcgg
     5581 agtgggcgcg cgagagtgtg ctggctgccg gtgtgtcgag cgaaaaggct gatcggatct
     5641 atgagctgat catggcgacg atgcacaagg cagttccggt cggcagagat gcagaacttc
     5701 tcgtcgatat cgatctgggc attctcggag caggagtcga tcggtttgat gcttacgaga
     5761 tccaggtacg tgaggaatat tcgtgggtgc ctgaatcgct gtatcgcgcg gcgcgcacaa
     5821 aagtattgga gcagtttgtg agtcgcgaat gggtctacag taccgagctt ttccgaaaca
     5881 aatacgagac tcgggcgcgt gagaatatca cccgttcgct tgctcgcctt cgcattgact
     5941 gaaactcaaa attcgtttca tccctcactg cttccgcctc aagactcggc aatcgctttt
     6001 gaacacagaa aacattgggt aatccttgcc acaggcgtat gatttgcgac atgcggctcg
     6061 tgcttgtcgt gattgcgctg gcattgctgc tcgtcgagtc gggcggcgtc ttcaggcctg
     6121 cgcgaacgac gaatgccgcg cccgcgaatc caccaactgc ttccgagcca aaaacgcgag
     6181 tcgatttcga tactcagctc aaaccaatat ttcaatcgca gtgcatgccg tgtcacttca
     6241 gcggtggaaa gatgtacgac cgcttacctt tcgataaacc cgcgacgatc aaaaaactcg
     6301 gcacacgcct cttcactcgc atcaaaggcg aacacgatcg caagctgatc gaagatttcc
     6361 tcgcgcagga ctgacaggca ttgtcagaac tcctgtcgat catgtaattc tttcggacca
     6421 tgacgacgac aaaagatcgg cgcccgctgg cgccaatcat cgccacggcc ctcatgccgc
     6481 ttggaatgat cgtgttgctg cgcgcggagg gacgaatgat ttggtgtgcg tgcgggcact
     6541 tcgcgttgtg ggtcagcgac tggtgcagct caaatacctc gcaacagttg ttcgatcctt
     6601 acagcttcac gcacgtgctg cacgggtttc tgtttttctg gctgatggcg ctgctgttca
     6661 agcgcatgcc gcgcgcgtgg cagtttttgc tggcgctgct gttggaaagc gcatgggaag
     6721 tttttgagaa cacgagtttc gtaatcaaca agtaccgcac ggagaccgcg gcactcggtt
     6781 accagggcga cacgatcgtc aactcgttgg gtgatcttgc ttgtgcgctg atcggttttg
     6841 ttatcgcacg acaactgggc gtacgtcgtt cgttgcttgt gtttgtagtg attgaagcgg
     6901 tcttaatatt ttggattcac gacagcctgt tgctacagat tctgatgttg atccggccag
     6961 tagaggcgat caagttgtgg cagatgtgcc agtagaagaa ctactgtagg ggtggctctc
     7021 cgtggccacc cgttcgtggg tgcgagatcc tcaattagac gaggttgttg tcgacgcggg
     7081 cgcaatcacg cgcgtgttga gatcttttag cgcggcgatg ttcttctcgc ggagctcgac
     7141 cgcgtcgcgc cctacctgaa tctcaaccga ctgtgccatc gccatcttct tgaacactgc
     7201 gtaaggcatc ttcgcttcca aaatctcgat cttcgtttcc catgtgcccg gcgtatcttt
     7261 gctgaccgga ctcatccggc cgatccgcag cgtttccgtg tcagtcacca gcgtcaaatc
     7321 ccgctgatct ggcggatgga agtggaccca gtctttgttt tcaaacacga cttgcagcgt
     7381 gacctgcttc acgtagttga cctgcgttcc cgggcaatgc agcatcacct cgatactcac
     7441 gcacgcatcc ttaaacttgt ctttgaaacc gtcgcaattg accttcattt ttcgcaggcg
     7501 catgacggtc tcgtagttga acccgtcgta tctcgtctcg atttttccac cgtgcttcac
     7561 gggctccggc gtttgcggtt tggctccagc gggttgaaac acaagcaccg cggcgcagct
     7621 caaaacaagc aggattcttc tcataacggc tctccaacac tcgaaattct gttttgagat
     7681 tgagtcaata cggggctcga accggtgaga aggctcacga gcaacatatc gaagcgggcg
     7741 aggtcatgtc aataaacaat ttcgggtctg ttagactgtc gccacactga cttgcgagga
     7801 cgaccatgac agcttctttt tctgatggtt ccctgactga tctggcagac cgcctccgac
     7861 aggcaaacga agaattcacg cgtcattatc ccggtgaaac cggtcgccgc cagccggttc
     7921 acacggttta cggcggcgcg cacctgttca aatccgactc ggccaaacgt ctcggctcgc
     7981 tggcccggcg ttcgttggat cagtttgcgc ccgactttct gacgtttgca cgcaccatcg
     8041 atctaccggg cgagagcgag ctgccggaat cgttcgacga agccactgag ctgctcgcgt
     8101 tcttgaatga agaaccggag cgcgcgcgca aagagaagaa aggcgcgtgg ctggcgcaca
     8161 cgatttaccg tcgtgtgatc gaaaagctcg agactgaacc ggtcgaagac tttcgcatcg
     8221 acttcgaaga tggttacggc aatcgtcctg acgacgagga agacggtcac gccgttgctg
     8281 cggctgccga aactgcagag ggaagtaaga aaggatcgtt gccgccgttc atcggcattc
     8341 ggatcaagcc tttcaacgag gagttgcgcg cgcggagttt tcgtacactc gacattttcg
     8401 tctctacgct cgtcgatcta aatcgtggac gtttacctga aaacttcgtg atcacgctac
     8461 caaagatcac gattcccgaa caggttgcga cgctcgcgac cgtactcgcg cgtctcgaga
     8521 aacacacgga tcttcctgaa ggtagtttga agtttgagat gatgatcgaa accacgcaat
     8581 cgatcatcaa tcaccagggc cagatcaatc tgccgttgtt gcttgctgct gcggaaggac
     8641 gttgtgttgc agctcacttc ggcacttacg actacactgc cggttgcaac atcaccgctg
     8701 cgcaccagca catgctgcat ccggcgtgcg acttcgcgaa gcacatgatg caggtttcgt
     8761 ttgccgggac gggtatctgg ctttcggatg gtgcgaccaa catcatgccg gtcgcgccgc
     8821 accgttttgt cgaaggtggt gcgccgttga cgcaggagca gatcgaagag aatcgcgagg
     8881 tggtccatcg cgcgtggaag ctgcattaca atcacattca gcattcgttg actaatgctt
     8941 tttaccaggg ctgggacctg catccggcgc agctacccac gcgttatgct gctgtgtatt
     9001 cgttcttcct tcaaggattg gaagcggcgt cagagcgttt gaagaacttc gtcgagaaag
     9061 ctgcgaaggc gacgctcgtg ggcgacgtgt ttgacgacgc cgcgacgggc cagggcttat
     9121 tgaattattt cttacgcgcg gtcaactgcg gcgcgctaac agaagaagaa gcggtccaat
     9181 tatcaggact gacgctcgag gaactgcgcg aagcgtcgtt tgtgaagatc ttgaagaatc
     9241 gacaggcgtt atgattgtgg taaactgctg aacactcacc agcatcggag ccttatgact
     9301 accaaactac aaactctcac catcaacgat ctggatgcca tgcctgcaag ggatggttat
     9361 cattacgaaa taatagaagg agaacttttt gtgtcctgct caccaggcct cacacatcaa
     9421 agagttttgg ccaatctcat aacgttgttt gtaatatttt tgcgggagaa tccgatcctg
     9481 gagtatgtac catcacccgg tttggtcttc gacgattaca gcggtgtcat tcccgatctc
     9541 gtcgtttttc ttgaccggag gagtgacgcg attattacgg gggagcgatt aactggacct
     9601 cccgacttgg tgatcgagat cctttcaccc gggccagcca atattcgacg agatcggata
     9661 attaaacgcc aactttattc taagcatgga gtgccggagt attggatcgt agatcccagc
     9721 catccgaggg tcgaacagta tgtcttgcac ggttcttctt tgaagctgat gcagactctt
     9781 cagcgcgaag atatcctgtc gagttccgca attccaggct tttcgtgtct agtaagtgaa
     9841 atctttagtc gcattggaaa gtgatgactt cattgaaact aatcatctcg atcgcattaa
     9901 caatggcact actctccacc acgttgatcg gacaaaagcc caacggcggc gatccggatc
     9961 tcgcgcgcaa gatcgcgcgt tttgcgccga ccacattgac cgctgacacg tcgaagctca
    10021 cgtcgaaaga ccggcaagcg ctcgagaaga tcatcgcggc ggcgaagctg ctcgatccgt
    10081 tgttcctgcg tcaggtctgg agtggtaacg acgcgctcga gaagaagctg caggccgata
    10141 agtccgcaac cggccaacag cgactccatt acttctacat caatgatggt ccatggtcgc
    10201 gtctcgacga gaaagaacca ttcatcgatg gcgtgccgcg tgagaagcca ctcaacgcga
    10261 attattatcc ggacgacatg tcgaaggacg agtttaattc gtgggtccag acactctccg
    10321 aagcagacaa gcaaaaagcg accggtttct tctggttaat ccggcgtgga tcagatcaga
    10381 aactgacgat cgtcccgtac agcgaggctt acaaagaata tcttgcaccc gcagcagcgt
    10441 tactccgtga agcggctgcg ctcacgacca acgcgactct aaaaaacttc ctgaacaagc
    10501 gtgccgatgc gttcggctcg gatgattact acgaaagcga cgtcgcctgg atggatctcg
    10561 attcaccaat cgatctcact tttggtccat acgagactta cgaagacgag ttgtttagtt
    10621 acaaagccgc cttcgaatct tacgtgacgt tgcgcgacga cgcggagaca gcaaagctca
    10681 caaaattcag cggtcacttg caggagctgg aaaacaatct tccgatcgat ccgaaatacc
    10741 gcaatccgaa gctcggggcc gcgtcgccga ttcgcgtcgt gaatgaagtc ttcgcgtcag
    10801 gcgaaggcaa tagcggcgtg caaacggctg cgttcaatct gccgaacgac gagcgcgtcg
    10861 tgaaggaaaa aggctcgaag cgcgtcatgc tgaagaacgt gcaggacgcg aagttcaaca
    10921 agacgctgat cccgatctcg aaagtcgttc tcgatccgtc agaacagtcg gcgctagctt
    10981 ttgactcgtt cttcacacac atcctctgtc acgagctgat gcacgggctt ggaccacaca
    11041 acatcaccgc cggcggccag gaaaccaccg tgcgcaagca gttgaaggag ctttactcgg
    11101 ccatcgagga agcgaaagcc gacatgacgg gactgtgggc gctccagtac atgatcgatc
    11161 gcggcatcat cgagaagagc atggagcgaa cgctctacac gacgtatctt gccagcatgt
    11221 ttcgttcggt gcgattcgga attaccgagg cgcacgggcg cggcgtcgcg atgcagttca
    11281 actacctgac tgatgaaggc gcgataaagt tcaacgaagc gaagggaacg ttcagcatcg
    11341 atcacgcgaa gatcaaagat ggtgtgacca agctcacgcg cgacctgctc acgctcgaag
    11401 ccgaaggttc gtacgacaag gcgaaagcga tactggataa gtttgcggtg atcagaccac
    11461 cgatgcagca ggcgctcgac aagctgaagg acgtgccggt cgatatcgag ccggttttcc
    11521 cgctcgcaaa gtagagccac aaaaggcaca aaaagcacaa aaagattttc ttcgtctttt
    11581 gtgctttttg tgccttttgt ggctctattt aaattgcttc ctgaagaacg tcgcgatttt
    11641 ccaggcgtcg atcgctttcc aggggagttc gccggtggtg tagtggccgc acgggagcca
    11701 gacgacgtcg agtgggagtt gctggcgacg cgcctccgca atcgcttggt gcgtgaggtc
    11761 gataggaaac gtgagatcgt aacgcgcgac gaggaagcgt atcggccggt gtcccatctg
    11821 cgccacgcgc cgcatgaacg gtagcgggct gatcggcgcc cagtaagtac gcagctcgtc
    11881 gagtgtcaaa tgatccgcaa agcccgcccg cacgtgcgcg gtcgagagtc cgtgccagac
    11941 cacatccgcg acgtagcctg aaacgtgatt gaaagtccct gctttaagat caggctcgtg
    12001 tgcgaaagcg agaaacgcta tacatgagcc gacgctcgtt cccagaattc cgatctgctc
    12061 ataccccttg ctctttaacc actgaacagc ggctcgagtg tcgagcactg cctgcctcat
    12121 cgattggatg gtgcgcccga tattgctgct gacgagatga tctgcgcgtt cgagctccgg
    12181 cggttttcgc tcgagatgat acggaagcgt cagacgcaac gccgacatac caatcatatt
    12241 gaagatccga catgcctcga cgtgactctg cggctgcgcg ttccactgcg gcagcacgac
    12301 aatggctttg cgtgaatttt tgtctttggt gggaaagaaa cgcgctcgcg ccgtgttgtt
    12361 ctctggcgag ggtgtgtgaa ctgcgcttgt ccaggttagc tgatcgcctt cgagacgata
    12421 gtcggtaatt gggggcaggg cgtagaaatt ttcactcgca ggcagtacgc gcgcggtatg
    12481 gctgcggaag agctcacgcg gatcattgcc attcacgtga tcgaagacgt attgcaaacc
    12541 ccattcaaag ggacggacga ggcgattatc atctctttgg tgataccagc gctcgcgccg
    12601 gtgcatgtag ttctgccaga aagacataaa aacggccacg gattcacaca gattatcacg
    12661 gttcagtccg atccgtgttt atccgtgcaa atccgtggct tattcttcgt ccttacaagc
    12721 aagtcgtcag cttgaagctg tgaatgtaag tcgtccagtt gaagtttcca ttcgccggtt
    12781 gatactgcgt cgtgaaaacc atcgtgcagc catcgaccgg gtcgacactg agcgtgctgt
    12841 aatcacccca acgcgtcagc gtggcggttt gaatcccgct gccgttcagg atcgtggtct
    12901 cgttgcccaa agtacctagc ggatcgagcg ggccacgata cgcataacga atactcggca
    12961 cgatcgtgct ggcattcgag atgttgtagc cgatagcgat accgccggtc ttgtccatcg
    13021 cagcgctgcc catccaacga aaatcattgg ttggtgcgaa cgtgccctgt tgtcgcagca
    13081 ctggcgccgc cgacgcgaga gtttgcccga cggcgtttgt cagctcatac cagcggacgc
    13141 ccacgcccgt gccactcgtt accgaatggt tgatcacgag cgcttcacgt gtaccaaggt
    13201 tgcgataact caagcgatac atgagtcgat cggccaggga atcaagcctg ttggtcgttc
    13261 ccggctgcgg aatgcaagtg ccgccgccac acgctctact aaaagccgca actccggcca
    13321 ctgccgttgg cccggtcagt gttcctgtgc ccgcgcccca gttaaccgca aacctccaga
    13381 agttgacggc tgtcgtcgtt atactcaaca ggaagtttgg tgaaccggcg ggcggcaatg
    13441 ttgttccttc gagatcggca ggctcgaggc tgtgatgcga cgtgctggtg ctggcgcatg
    13501 cttgcctcgc cacaccgcca accagcattt gcgcgcgttc aaacgcacaa actgtgttgc
    13561 cctggaaggt gctgccattg cggaacatgt tgtaggtgat gtagtaggcg tcaggccaaa
    13621 cgcccatctt cggataatcg ttgaaactcc ggttgtacga aaacgcatac cggaaatatg
    13681 tgccggtcgg atccggcgag gtcgaaacgg ccacgcattg cgtgaacggc gttgccgaca
    13741 ccgcaaactg cgtcagtatc caacgatcgg ccaactgatc gtattgcacg atcggatcgc
    13801 cgtcgttatc ggactggcag cgaccgccga agccctgcca taccacgttt ccctgttttg
    13861 ggaatccggg cgcgagattg aaagcggtaa tgttattgct tgcatcgcgg gtcaagttgt
    13921 agaccgcgta acgcaggttg acccactgca cataatgatt tggtccaacg tcgccagtag
    13981 tgtctggtgg cgcaacgcgt gcggctggat cgacaggtgt gccgtttgtt gcggccttga
    14041 tctcggcgag cgcagacggc gcgctgacgc ttttatctgc cagcgggtcg gtgaagggcg
    14101 tgttgttgtt tgaaaccttg tccggtttgc caggttcgaa gtctttgccg ccgcgagctg
    14161 gttccggttt ctcgccggtc tcaaacattt cgcgtagagg ttttgttgtt ttgtcgaatt
    14221 tgatggggta actgatctcg gcccggtgtt ccagagcctt ctcgctctgg gcttgggccg
    14281 tcaggggtac gaagctcact gtacacagga tcaccatcat tagtaaggca tgaatctttt
    14341 tcactgaatc ctccaaaaat tacagggcat gtgagttacg ggtagcagta gtacttggga
    14401 gtggactaca gcgtctgatt tgcgaaatta cagcaacaac agagcgagaa ggtattttgg
    14461 gaggcggaca gtatacaact attttggttc gtagtgtaaa gacgcttgat gtccgacatg
    14521 ctttagcttg tcggattgcg cgacaaactg aagtttatcg gacatttcac tactgcgctt
    14581 aacggggctc gatgaggcgc cgaagtattc ttggtagcag ggggagggaa ttcaagactg
    14641 agccggtggt gaagcgtcgg gcgaagtcat ggaaaagctg gcccgctcgt tttgaatatg
    14701 gaaaccacca cacatccgct aagcccggca tcgcgttgac atgaatatgc tgtgcagaaa
    14761 cgagctccaa cagtccgaga ctgccgtgcg tccgcccgta accgctttgc ttcacgccgc
    14821 cccacggcgt ttgcgccacc gcgtgtgtgt aaacaacctc gtttatcata actgtgcccg
    14881 cgtcgatctg ttcggcaatc cgctttccgc gttcgagatc acgggtgaag acgctcgccg
    14941 tgagcccgta aatactgtca ttcgccaggc gcactgcctc ttcgtcggtt cggaacgtca
    15001 tgatcggtaa caccggaccg aaggtttcat cacgcattag tttcatcgag tggtcgacac
    15061 cggtgacgac ggtcggctcg tgaaaccaac ccgctgactc attcaggcga tgaccaccag
    15121 cgcgcacctc ggctccgcgt tgaatcgcat cgctcacgtg atcttcaaca atctctagct
    15181 gctcttgatt ggtcatcgcg ccgacgtcga tatcgccagt cgaaggcttg tcctgtttca
    15241 gccgcttcgt ttctttaata accagctcga cgaacttgtc ggcgatggac gcatgcacgt
    15301 agcaacgctc aatcgaggcg caggcctgac cggagttgca gaacgcgccc cagatcgctg
    15361 cgcgcgcggc gttctcgaga tttgcatctt caaaaacgat catcggatcc ttaccgccga
    15421 gctcgagcac gaccggcgtc agatgttttg ccgcagcttc ggcaacgcgc ttgcccgtgt
    15481 tcacactacc ggtgaacatg atcttgttca caccagcttc gacgagcgcc gcgcctgtcg
    15541 agccatcgcc ggtgacgatc tcgagcagtc catccggcag ttgagcttgc ttgaagacgt
    15601 ctgcgatctt gagggcagtt aaaggtgtca actcgctcgg tttcacaacc acagcattgc
    15661 ctgccatcaa cgccatcacg acttcgtcga gcggagtggc ccagggaaaa ttccacggcg
    15721 agatgattcc cacaacgccg agcggtttgt agacgatgta tgacgtacgg gccatatagt
    15781 tgtactggcc caaaccgacg cgtttgcgtt tgaggatctg tttcgcgttg tcggcgaagt
    15841 agtgcatgag atcgagcgtg ggcacgatct ccatcgcgat tgcttcagtg actggtttac
    15901 cggtttcacg tgagaccagg tttgcgatct cgtcgacttg ttccagcacg acctcgcgcg
    15961 cacgcaggat gaagcgagcc cgatcgtggt aggagagctt ggcccatgcc ggttgcgccg
    16021 cgcgcgctct cgcgacagca gcagcaactt ccgtctcgtc cagcaacggc acacgaccaa
    16081 tctcttcacc ggtcgatgga tcaactgaaa tgagcatcgc cgaattatac agaaccgagc
    16141 gcggtagcgc cgggtcaaac gaagttacga ttatgatcgg gaaaccacca cccggcgcta
    16201 ccgcgctcgg ttctgttaca atgcggccac aaggagaatt tgtgatgatc aggccccagc
    16261 tcgaggagtt tatgcgccgt atggcgccca aatccgtcgc tatcattccc ggtgcacacg
    16321 acaccacccg ctcgaacgac acgcattatc gcttccgcca gaactccgat ttcttctatc
    16381 tcaccggctt cgaagagccg gatgcgctcg cagtaatcaa agaccagaag tacacgttgt
    16441 ttgtgcggcc gcgagatccg gaaagagaga tctgggacgg gcgtcgggcg ggagtcgaag
    16501 gcgcgaagag cgagtttggc gcgtcagagt catttccgat cgcggagttt gaatccaagc
    16561 ttcgcgagtt tctcgacttc acggacgtgc tttactaccg gctcggtgat gatcgcgatc
    16621 tcgacaacgt cattctcaaa gagatctcaa acatgcgcgc gattaatcgc aagcccgtgc
    16681 atccgccgca gacgatcatc gacccggcga cgatcgtgca cgagatgcgc gtactcaagt
    16741 cgcctgaaga gatcgagatc atgcaaaggt cagccgacat tgctgccgaa gcacattgcg
    16801 aggcgatgaa ggcagcgaag cctggcatgc aggaatatca gctcgaagcg ttgatcgaac
    16861 aggtattccg gcggcatggc gcatcgggtc cggcttacac gtcgatcgtc ggggctggtc
    16921 caaacgcgac cgtgctgcac tacatcaaca acgatggtga gctgcgcgac ggcgacttgt
    16981 tgttgctcga tgcgggtgct gagtacaagg gatatgcttc cgacatcacg cgcacatttc
    17041 cgatcaatgg tcgttacacc aaagcgcagc gcgagattta cgacctcgtg ttgaaggcgc
    17101 agatggagtg tgtcgagatg gtgcggccgg gcacgacgca cgagcagttg aagcagcact
    17161 cgatcgaggt gttgacggaa gggatggtcg agctcgggtt gctgaaaggc aaaccggaag
    17221 agctgatcaa ggagaagaag tacgagcagt tttacatgca cgggctcggg cacatgatcg
    17281 gcatcgacgt gcatgatgtc gggcgatatt acttcgggac agagtcacgc gcgctcgagc
    17341 cgggagtggt gatgacggtc gagccggggt tgtacatcgc gcctgatacg aaggacgtgc
    17401 cggagcagta tctcgggatc ggtgtgcgga tcgaggatga tgtgctgtgt acgaacaatg
    17461 gaccaagggt gttgacgcac aaagtcccca agcaggccga ggagattgaa gagttaattg
    17521 aacgcggata acgcggattg aaaacgcgga ttaagtcatc ttctggatga tggcgtccgc
    17581 gaattctgct gttttggcgg tgccgccgat gtcgcgtgtg cggacctttc cttctcggaa
    17641 gacgttgagc atcgcggttt cgattcgatc ggcggcttcg cgttcgccga ggtagcgcag
    17701 cattagaata ccggattgca gtaatgctgt tgggtttgcg atgccctggc cggcgatgtc
    17761 gggtgcagag ccgtggaccg cttcgaagac cgcgcctttc tcgccgatgt tcgcgcccgg
    17821 cactaatccc aggccgccaa caagtcctgc acacagatcc gagacgatgt cgccgtaaag
    17881 attctcgagc agcatgacgt caaactgctc cggtcgcatc acgagctgca tgcatgcgtt
    17941 gtcgacgatc ttgtcgtcag cctcgatgtc gggataatcc acactgacac gtcggaagca
    18001 gtcgagaaac agaccatccg acagcttcat gatgttcgcc ttgtgaaccg ctgtaatttt
    18061 cttgcgcgac tcgcgtctcg catgctcaaa cgcaaaacgc gctatgcgcg tcgaagcctt
    18121 ctcggtgatg atcttcaaac tctcaaccac gcccggcacg acgatgtgct ctaaacccgc
    18181 atacaaactc tccgtgttct cacgcacgac gatcagatcg agctcgggat aacgacacgg
    18241 cacattcggt aacgcgcgca ccggacgcag gttcgcgtaa agatccaacg tcttacgcag
    18301 tccaacgttg acggaagtga agcctttgcc gaccggcgtc gtgattggtc ccttgagcgc
    18361 gaccttattg cgcacgatcg aatcgagcag ctctttcggt agcgtttcgc cgaatttttc
    18421 gagcgcttgt gcgcctgcgt aatgagtctc ccactggacc tcaacacctg acgcttcgat
    18481 gattcgcact acgtttgagg cgacttcagg gccaatgccg tcgcccggaa tcagtgtgat
    18541 tgcatgcatg gtcacagcat acaataatag gtcatatagg cctacaggac ctattgatgg
    18601 gttttgctgc aagtgtccga taaactttat agtttgtcgc gacattccga caagctaaag
    18661 cttatcggac atcaagtgag agatcggtac gacattgtca tcgttggagg cggcgtcatt
    18721 gggctaacca ttgcgcgtgc gctggtgctg cgtggtgtgc gtgatgtttg cctcgttgag
    18781 cgtggaaacc tcggcaaaga agcgtcgtgg gcggcagccg ggatgttgct gccgcaggtc
    18841 gaagccgaca cgagcgacga gtttttcaat ctcgcttgtc agagccgcga tctatatccg
    18901 gcttttgcgg cggcgttgcg cgaagagact ggaatcgacg tcgagctcga catcaccgga
    18961 acgctttatc tcgccttcac ggaaacggat cgcgaagaaa ttgaaaaacg atacgagtgg
    19021 caaacacgcg cgggattcgc tgttgaacga ctcagcggcg cagagggcgc gtgaattgga
    19081 accgtgcatc tccgaagcga cgcgcggcgc gttgcgattt ccgaaagaca ttcaagtcga
    19141 gaatcgtcga ctgctcagcg ctctcgcaaa cagcgtaacg aagctcggcg tcgatattgc
    19201 aacccaaaca accgtcgagt ctgtaaccat tgagggaaat cgcgtgcgtg gtgtgcagac
    19261 atctcgtggc gcgatcattt gtccagtagt ggtaattgct gcgggaacgt ggtcgagttt
    19321 cattaagagc gcgccggttc cctcgatcga gcccattcgc ggccagatga tctgtctcga
    19381 agcaaagccg cagctaacac gtcacgtgat ttacagcccg cgcggctaca tcgtgccgcg
    19441 ccaggatgga cgtttactgg ccggctcgac gagtgaacac gccggtttcg caaagtgcgt
    19501 cacagccggt ggaatcgcgg ggattctgcg gaactcgttc gaaatttcac ccgcaacatc
    19561 ggatttgccc gtgatcgata cgtgggctgg tctccggcca cgggccgctg acggtttgcc
    19621 tgtcatcggc ccttgtggtg aaattgacgg gctcttttat gcaaccgggc attaccgaaa
    19681 cggaatccta ctcgctcctt taacgggtga actgatttcc aaagcaattg tttcctctca
    19741 aagatgatta aagctgtttt tttatttgtt ctactgactg ctatctcgac gtctgcgcag
    19801 ggcctcgctc cggccgtcaa acccgacggc aagcctgaca agcgttcggc gcaggaatta
    19861 tttgaagaag ccaacggata cctcggacgg cgctttcagg aattcaacag acaaaaactt
    19921 ccgtacgatc caaaactcga agcccagacg aagaaagagc aaaaagatct cgcagtgcga
    19981 aacgcagcga cactgcaggc gcgcggctct ttgaagggcg acgatcttta ctacctcggc
    20041 atgcttcatc atctctctgc tgacgctgac gcttcactca ctacgatgcg tctgctgcta
    20101 aaaggcgacc ccgacggtca aaaagcgcag gctgctcgta acgtcgtcgt tctttacgcg
    20161 gtcaaaaagg atctcatctc ggaagcgacg gcagcagtcg aaacgtacag acgtcaccag
    20221 cctcaaagcg cggacgatct ttatcgcatg gagtttctga tcaccgactg gtacctgcgc
    20281 gcgaaagact acgggccgat gaccacgcac gcgaagcaaa tgctcgtcgc ggcgaagaag
    20341 tttatcgaga cgaataaaga cgaggtcgtc aagcgcgatg agatgttgtt gaagtcgagt
    20401 gtgctgatcg ctgattccta tttgaaaaca gatcagaaga agttagcgat tagcgcgctg
    20461 gcagacttgc gacgcatagc gattcagttg ccgtcggcga atctctataa agacgccacg
    20521 gtgcgtttaa tgcggctcga tcccgagttg aataccgcac agcttttcga cgatagtctg
    20581 acgtcgaaga atgctgtatt accggactta atcgcgaatg actggatcga gcagcagcag
    20641 aagaagctga cagactttcg cgggcaggtc gtgttggtag atttctgggc gacgtggtgt
    20701 ggtccatgcc gttacacgct gccgaatctg gcgcgctggc agaatgcata taagaccaag
    20761 ggactcgtga tcctgggcgt gacgaagtac tatggacgcg gtggcgggaa agagttgact
    20821 gccgacgaag agcttgcctt tttgcgcgac tttcgcacgc ggaacaagct gccgtacgga
    20881 ttccttgtcg aggacggcga taagaacgaa tttaactacg gcgtattttc gatccccacg
    20941 tcgtttttga tcgatcgcaa gggtgtggtc aggtacatct cgctgggtgc gagtgaagag
    21001 gagattgcgc gactaggtga gttgatcaag caattagttg aagagaaata gttttagaca
    21061 ggatttacaa gatttttcat gataatcatg tagcatcttg taaatcctgt taacaactgc
    21121 tttaccaaac atggacgaca acggaattac aatttttcgc attttgatct cgttcgcggc
    21181 gggaattctc tcgtttctct cgccgtgcgt tttaccgctc gtccccggct acatctcgct
    21241 gatgtcgggc gtcagtatcg acaatcttaa agaaggcgtg agcagtcgcc gcgcggtgat
    21301 cgtcaactcg ctcgcgttca atgccggcct gtcagtcatc tttctcgtgc ttggtacaac
    21361 tgcgggtctc gtcggctctg cgatcacgag caatccgtgg gtacggataa tcggcggcct
    21421 cgtcatcatc gcgtttggcc tgcaactgat cgggctacta aagatcggcg cactctataa
    21481 agacacacgt ttcttctcga gcgagaagcc gcgtggtgtg ctcggctccg ctgctctggg
    21541 gatggcgttc gccgccggct ggacgccgtg cattggtcca attctcggcg gcatcatcgg
    21601 tctcgccgcg accagcggtg gctggcgcag tggcctcatc ctttcagcgt tttactcggc
    21661 gggcctcgcc gtgccgtttc tgctgacggg cctcggtatc aatcagtttc tgggtttcta
    21721 taaaaacttc cgccggcacc tgcacaaggt cgaggtcgtg tcaggcgtcg tgctaattct
    21781 tgtcggactg ctggtgatga gtggtcaatc gactttgctg gcgagctcga aattcatggc
    21841 gctcgttccg aatgcggaag gtttgctgaa tcgctggttc aagactgaga ctccgccagc
    21901 caaacagacc accgcaactg caaacactaa cttcgaaatg gcgcccgacg tcgagtttca
    21961 aacactcgca ggcaaaccgt ttcgactcaa agagcttcag ggccaggtcg tgctgctgaa
    22021 cttctgggcc acttactgca ttccgtgtcg cgaagagatc cccgctctca acgcattgca
    22081 gcacgagctc cagccgcagg gattgaagat cgtcggtgcg tcgctcgacg acagtgccga
    22141 cggtgtgaac gcttaccagc aggaagtcgc gaagtttgaa tacgacgtct tgcttggtgg
    22201 tagtgatgcc aaggtaaaat tccagcagtc ggtgttgccg acaacttatc tgatcgatcg
    22261 ccagggacgc attcgccaga agataatcgg ggcccgcgac aaggccgcgt gggaagccgc
    22321 ggtcaagccg ctgctggctg aaacgccggc aactgcgagc gccgcaaatt aatggagtag
    22381 taccatgaag aaactcttat tcgccgtctc tattcttgca ctcgccatct cgccttcggt
    22441 tgcgcgtgcg caggccggtc acgagtattc gccgctcacc gagacgacgg tgaactacaa
    22501 aaactggaag ctgccaaatc taacggctga gaaatcggaa gacttgcgcg agctgatggc
    22561 gggcaagaag ctcgtgatga tcgtttactt tgcgccatgg tgtggaaact ggaaaaacga
    22621 agcgcccatt gccgcgaagt tgtacgagaa gtataaggcg caagggtttc aggtgatcgg
    22681 catcagcgaa tacgcgtcga aggacgacgt ccgcaactat ttcggcaagg atggtccacc
    22741 gtatccggtt gtgactgagt cggagacgag agaagacaag cagaagactg cgcattatgg
    22801 gtaccgtcaa ctcaccggcg acaaacgcaa ctggggctcg ccgtggaaca tctttctcga
    22861 gcccgagaag gtcaatgcca aaggcgacgt gctcatcgag aaggcgtttg ttgttaacgg
    22921 tgaattgatc gaagacgagg tcgacaagtt catcgcgtcg aaagtcggtg gcgccagcgc
    22981 cgtcatccag ccttgtaaaa attaatccgt gtttatccgt gaaatctgtg gcaaaaaaac
    23041 cgaatgaagc ttcgtatcct gtatcacggt cactgctttg acggtgtcgc ctcggcggcc
    23101 acgttcacgc gcttctacaa agaacgcatc catcctgaag ctgtcgtcaa ctacaccgga
    23161 ctgctgcacc ggccaggcaa tttgtttgac ctcgggatgt ttgattccga cgaaaacgcg
    23221 atcgtcgatt tcaaatatgc agcgtcagac aaactgacgt ggtggttcga tcatcacgag
    23281 tcggcattcc tcacgcctga agacgaagct cactttcacg ccgacaagtc aggacggaag
    23341 tttctcgatc ccacgcgcaa gtcgtgcacg gagtttattg ctgacgtcgc gcacgagcgt
    23401 ttcgggttta atcccgagcc gatcgagtcg ctggtccact gggcacacat catcgacggc
    23461 gcgctatacg agtcgccggc gcagtgcgtc gagttgaaag agccggcgtt gcaactcatg
    23521 caggtgatcg aagccgatcc tgacgatgcg ttcatcgagc agatcattcg cgagctgacg
    23581 acgcattcgc tcgaggaggt ggcgacgagt gcggaagtgc aacgtcgttt ccagccgata
    23641 ctgaaacaac atctcgaaac gctcgaagtc atcaggaaga aagcgatcag cgcgcacggc
    23701 gtggtccagt ttgatctggt tgacgagggt tacgagggct tcaacaagtt catcccgtat
    23761 tacctgcatc cggaagcgac ttactcggtt gcactcacgc gtgggtcaca gcgcacgaag
    23821 atctcagtgg gctcaaatcc gtgggcgccc gtgccgcgca cgcacaacat cgcgaagatc
    23881 tgtgagcgat acggtggtgg cgggcacgcc gtggtgggcg ccgtttcgtt caagcccgaa
    23941 gaagtcgaag aagcacgcca ggccgtgcgc gaaattgtag aggagttatc aaggaagtct
    24001 tagggtatgt tttaccaaag ttttagccgc ggatttacgc ggataaacgc agatctgcgt
    24061 tcatccgcgt ttatctgcgg cttacttctt ttttgtgcat gtgtagtgaa tggggcgacg
    24121 ctgccgccgg gattcactga aacatcaatc agcggtctca acaatccgac cgcaatggag
    24181 atcgcgccgg acggccgtat cttcgtctgc cagcagggcg gaagcctgcg tgtgatcaag
    24241 aacggggcgt tgctctcgac gccgtttctc acgctgaacg tcgatccaaa cggcgaacgc
    24301 gggttgctcg gcatcgcttt cgatccgagc ttcgcgagca acaattttct ctacgtctat
    24361 tacaccgttc cctccaaccc acgacacaac cgcgtcagtc gcttcactgc aaacggcgac
    24421 gtcgtcgtcc cgggtagcga acaaatcatt ctcgagctcg acaatctaag ctcagcaacg
    24481 aatcacaacg gcggcgccat tcacttcgga ccggatggga agttgtatgt cgcagtcggc
    24541 gagaacgcga acggatcgaa cgcgcaaacg ttgagcaacc ggctcggcaa agttttgcgt
    24601 atcaactcag acggctcgat cccaaacgat aatcctttct tcaacaccgc cgcgggagac
    24661 aatcgatcga tttgggcgtt tggcctgcgc aatcccttca cgttcgcttt ccaacccgga
    24721 acgggacgaa tgttcattaa cgacgtcggc gaggtcacgt gggaggagat taacgacggt
    24781 atcgccggat caaattacgg ctggaacctg agcgaaggtc cgacgagcaa cccgcaattt
    24841 cgatcgccgc tgttttctta tctacacggg ttcagtgcga cgacgggctg cgcgattgca
    24901 ggcggcgctt tttacaatcc ggcgactgtt caattcccgc agagtttcgt agggaagtat
    24961 ttcttcgcgg atctgtgtac tggctggatc cggctcttcg atccatcgac gggaacggcg
    25021 tcggattttg cgtcgaatat ttcgaatccg gtggatttga aggttggcgc tgatggcagt
    25081 ctttattatc tcgcacgcgg tgcgggtgcg gtgttccgcg tgcaattcaa cgaaccgatg
    25141 ctgatctcag aggccggttc ggatagtgcg gtggcgctcg actccgtgac gttcgttcgc
    25201 gatccgttcc cgttgacgaa tattttcaac ttcagcgcgg atcagcgaac gcgcttgatg
    25261 ctgtttggga tgaacatggc attgatagcg ggtgagaata gctcggcggt gacggcacgt
    25321 gcggaggacg cggcggtgaa tgtttatccg ttaactgtcg agttcgttgg gccgttaccc
    25381 ggggccgatg gcgtcacgca gatcgtcgtt cgattgccgg ataacacgcc ggcaggtcag
    25441 acgttgtttg tgagcgcgac gttgagaggc aggacgacca acaaggtccg cgtgcgaatg
    25501 caatagaaat ttagttatcc gcagattacg cagatttgtt taaaacatct gtgtaatctg
    25561 tggataaaaa accagcctgg atccctaatc tcttgatctt gcaattgagc gtcgtcactt
    25621 tcactcccag cagtttcgcc gcacgccgtt gattgccgcg cgtccggtgg agcgcctcac
    25681 gaatgagctc actctcgtag cgctgcacct cttcctgcag cccaagccgc gccggtgccg
    25741 gttcctgctg gctcgcaagc gactcaactt cgcgcagtaa cgtgatcgca gcttctctaa
    25801 gcgtgtcgag attgtgaata gaactactca tcacattaat ccttgctcaa aagttctctg
    25861 aagaacgcca ccgcgcgttc atcgccagtt tgcccgagca cctgaatcgc gagcttgcgc
    25921 accggcatct ttggatgatt gcgcgccgtt cgaatcagaa tcgggattgc ttcatccttc
    25981 ggacgacgcc caatcgcaat caccgcctgc ttttgaattt caatatccgc atcacaatcg
    26041 accgtgctgg tcaacgcgcc gagactcttc tgtcctgcga tgcgaccgag gttggcaatc
    26101 gcgctcttgc gcagctccat gtccttctca gattcggcaa cgcgaatcag ataagtcgca
    26161 gcagcgtcgt tgttgtcgtt acgcgcgatg ccggtgagtg cacgtcgttt gagatcgcgc
    26221 gtggtcatcg tttcgaagag gttctgtagc aacggcaaca ccgcagcgga gcggctcatc
    26281 gcaagcgccg acagcgccgc gtttcgtgct tcagtgcttt cctggttgtt gcgaacgatc
    26341 tcagtgaaga gcgtgttttt tgcggctgtt tcgggcgtgt tgccgagcca gtaaatcgcg
    26401 cgggagcgca tcggctcagc gatgggccgt cgaatcatct cggtcagcat cgcatcgaca
    26461 cgcgcgtcgt cgtgcaacgc aatcgcgaac gtggcgcgct cggcgagacg attcacttcc
    26521 ggcgcaggcg cgtcgacgat cgacttcagg tagttcagac tctcttcgtt accgatgtgt
    26581 ccggcccagt aaaccggata accgccgtac tcgcgatcgc ggcgcaggtt gtaaacctcg
    26641 gcgcgaatga acgtctcgcg cgtcgtgtcg taaagaagga agagaccgag ctcgcgcgtt
    26701 tcgtggcgcg ggtcgaacat caccgacgtg ccgtctacag acatgtaaac accgccggca
    26761 ctatcgacca cctcgtaatc gatcgcgaca ccgggcctgg cttcgaactg gtatccgacc
    26821 cagaaccggc cattcgtcgc attcgcttta ccagcgacga tcgcgccatc gatcttggcc
    26881 ttcagattgg cgccgtcaac cggagtaaaa ctcttcgtct gccccagcac gacaacgcat
    26941 agcaacagaa ttacagctaa tactttcata actgaatccg tatttaatcc gtgtaatctg
    27001 tggtctattt aagcagctct tccaaaaact tgagcacttc aggatcttta ctgcggctca
    27061 accagccaat cgcgcgtttg cgacgctcca tcggtgcctg gggactgcgt gcaatctcga
    27121 tcatcttgcg gatcacgcgc tggtcttcca ttctgcttcg ggcggtagtc gtgtagacac
    27181 cttgacctga gtccatcacc attggcgccc acatcccgct gccgtaaccg agcgagttga
    27241 tgatctggtc tttcagctcc tcgttttttt cgatgtcgta gagattgagg agaaactcga
    27301 tcgctttatc cccaccgcga cgcgagatcg cgctgatcgc tgctttgcga agctcgacat
    27361 tgtcagagct gcgagcaatg tcaaaaagct tcgtgcccgc gcgctcgctc ttgcgattag
    27421 cgaaaccgaa aatcactgcc ttgcgaatct caactgtttg atcgccgtcg tagatccgga
    27481 agagcgcatc gaccgcggga tcgccgggac gactggcgat gctggcgatg gccaccttac
    27541 gttgcggaat cgttctgctg ctgttcgtgg caaactcgct cagcaccgtt atcgctcgct
    27601 cgcccgtgtg tttgctcagt gcgtaaagcg acgcttcgac gatcgagttc tcctgcgagc
    27661 tcatggcgaa gtcgcgcagt gaatcaacca cactgtcgtc gttggttgcg ccgagcgcgg
    27721 agatagcgcg agcacgcagt ttcagatccg cttcattgcg cgcgacaccg aggatcacgg
    27781 gcgtgactgt cttgccgcca ttgcgaccga gcaacgtcaa cgccgcaccc ttgcaacgca
    27841 cagtctgcgt cgaacccggc ttgagccatt cgctcgccgc catgattccg cgttgcacgt
    27901 ctgtttgaaa aagtgcctgg agtacgacga tcttgaactc acatggatcg ttgctgttgt
    27961 cgtcgtcttc atcgtcggcg ggaccaaccc cgattgcgat accttcaacc ggagcaatgg
    28021 gagtggccgg cgctaccgtt acctgaggaa ccgttatcgc tcgtgagtaa attagtggtt
    28081 gttcgattgc gggcggcgcg atcactgcct ggccctgcac tgttctgacg agatctgcgt
    28141 aagcggccgc ggaagctccg ggcacctgcg ctaacaacgt gcgcgcatcg tctttccagt
    28201 tcgtgctcgg atacttctga agcagccggt cgatcgtcga gcgacattga tcgtacttcg
    28261 ctaacttctg ttcagcgtac gctttccaat aaagcgcggc gtcgatgttt ttctcgttag
    28321 gaaattgcga gacatactcg tcaaacttag cctgcgctct ggcccaatcg ccgtcagtga
    28381 tgaggtcgcg tgcgctgcga aagacagtcg tagcttcacc ggtgccttga gaacggccag
    28441 aacccgagcc gctaccgcta cccgaacctg agcccgaacc agttccctgt tgaccgcgcg
    28501 cgatacccgc ggtcatcaaa acaagcacgc aaaagagcaa cgcgacgcgc ttcgttgccg
    28561 taatcgatgt aaggttcttc aacatttctt tagcctctac tctcattaag gtaacaatat
    28621 acttggcgca tgaatgtccg acatgcttta gcttgtcgtt gagatttaca acactcaaga
    28681 aatgatcctg gttgtcagcc acacactcga gagctccatc agacgacaag ctgaagcatg
    28741 tcggacattg atgcgccagg tatatcattg ctctcattag cgattgttgg tgccgtggtc
    28801 ctggtaatcc agtaacgctg ccacgatttc ggtcttttgc acacgctcct taatttcccg
    28861 cacgtcttga gggcgtgcac gatcgggcag attcgcgatg tcaatcagga acggttccaa
    28921 atccgcgagg agtgttttag tcggaaactt tgctttcatc tcggcgtcac ggcgcaggac
    28981 gacgttttcg ttaagcagtc gacgcgagag tgctttgtcg taagtgacgt cgatctcttc
    29041 gtcagtgtcg gagatctgaa cgtttttaat cgagcgcaac agcatcttgc tttgttcgat
    29101 gtgcaaggct gtatcctgct cgtccatgtc gaggtaagcg agctctgatg aaagaacatc
    29161 agattgatcc gttacggttt gaacgattgg tggtttgact cgtgatcttg gtttctggcg
    29221 ttttggtgcc gcggattgga cggattttgg aagcggatct gggcttgggg tagttgagtg
    29281 tgttgttgct actgtcggaa ctggagtttt gggtttcgat aacaggtaga tggctccgac
    29341 tgcgagcgcg attaacaata ctgcgatcgc gcttgcgagt gatgttttgc cgaacagact
    29401 cgcgagccga cttcgttttg gcgtattagt gtgcgcggcg attctcgtcc agaggaacgg
    29461 aggtacttcg atttcgcgat cgtaaagcgc gaagatctcc tgttcggctg tcagcgctgc
    29521 gaagctttca gaacactcaa tgcaggcaga taaatgcatg tcgacctgct cctgatcgtg
    29581 cggcgccagc tcaccgtcca gatactcttc caacaactca ctgcaaactt cacacttcat
    29641 aaccaactcc agcgtaggga ctgatctttt ttctcatctc cgcgcgggcg cggtgcaaat
    29701 cagtcttcaa cttcgcaagc gagaaacctg tgatggcggc gatctcttcg taactgcact
    29761 ggtgcagcac cttgagcgtg aacaccagtc gcttgtcttc atcgagtgcg gccagcgagc
    29821 gccggacaag cgcgttcaac tccttgctca atagcccatc gaccggcacc ggtttgtcgt
    29881 cgcttaaatc catgaccgac ttatccgcga gatcgacgtg attcttcgcg cgggcgcgtg
    29941 cccggagcga ctcgcgcgcg acgtttctcg caatgccaaa cagccaggtt gagagtttgg
    30001 tttcggcgcg catggtgtgg atcgctcgga aggcgcggac gaaggtttcc tgcgtcagtt
    30061 cttcggccag cccacgatcg ttgaccatgt cgtaaatgaa actaatgacg gggcgcgaat
    30121 acctctcgaa aatcaggcgg aaggcatccg catcgccctg gcacacgcga gttaccaggt
    30181 ctgctgaagt gttcatcaga gttcccgcga acgcgccagt catcgctctc ggtaggtaag
    30241 tgccaggaaa gggctaaagg tttcaggaaa aactatccac agattacaca gatcacacag
    30301 atagcaagca aagcaacatt tgaaaatctg tgtaatctgt gcaatctgtg gctaaatatt
    30361 ctttgggtcc acgatcttgc cggcgatcgc ggaggcggcg actacgtacg gtgaggcgag
    30421 gtagagcttg ccgggaccgc tgcgacccgg aaaattccgg ttttgcgcgc tgacggtcac
    30481 cgtctccgcg ttcggcgaag cgcccggacc ggcattgatg catgcaccgc acgacggatc
    30541 gatcaactcc gcgcccgccc gctcaaaaag gtcgatataa ccacgctgcc gcgcatactg
    30601 cttgatcttc tgcgagccaa actgaatata gaggtgtaca ccttccttca cgcgcttccc
    30661 attcgcgagc gcgttttcga gcaccgaggc gtacatgtcc atgtccgcca tcttcccgcc
    30721 cgtgcatgag ccgccatacg ccgcatcaat acgcacctcg ccgtcgagct cctcgatcgg
    30781 aatgccattg cgcggatccc ccggcgtcgc gaccatcggc ccgatcgcgt cgagatcgat
    30841 ctcgaaaacc gccgcatact ccgcatcctc gtcgctataa acaaatcctt tgcgaacctg
    30901 ttcaggatcg aggccgcgtg tacgcaccac gtaatcgagc gtcacttcat caggctcgat
    30961 gattccggtc gtgccgccgg cttcgaccgc catgttcgtc aacgtcgcac gctcgtccat
    31021 cgcccagttc tgcaaatcct cgcccgcaaa ctccaacacc ttcccgatgc ctttgctcga
    31081 cttcatgtac tccgacgcga gtatgaaaag catcacgtct ttcgccgcga cgtcggcgcg
    31141 cttctttcct ttcaaaacgt agcgcacagt ttcaggcaca cggatgcgaa tgtctttcgt
    31201 gtaccacgcg tttgccatgt cagtcgatcc aacaccgaac gcgaaacagc cgagtacgcc
    31261 ggccatgcac gtgtgtgagt cagtgccgat aacgatctga cccggaaggg cgagatcttc
    31321 tacgaccgcg ttatgacaga tcgcctccga gccgccttca ggactctcgc cgtaaagcct
    31381 gatctgctgc tgcgtagtga aactctgctg cgtcgttgcc aaaccttcag ccttctcgag
    31441 cagacccatc tcacgatgct tcggcgacat cacgcgcccg agaaacgtca ggtgatcgcg
    31501 aaacgcaaac accgattcgg gctcggaaac acgggcgtca gggcccagcg cctgtttgaa
    31561 cagcgactcg gccatcggcg tcacgtactc gtgcgagaag cgcacgtctg cgagcgcgaa
    31621 caacgcgtcg ccgggcttca ctgcttcgac gccgatcttt ccggccgtca cgaatgcatg
    31681 ccgcgcgatg atcttctcga cgatcgtcat cggacgcgcg ggcgtttcga tcggcggtgg
    31741 cgatactttt ccgttgagcc gcgctttgtt gtagttgaag agcccgccgt gcgtaacgat
    31801 gtcctgcgag atcggatcga ttcctttagt gaactcagtc agcgggatct cctcgccgcg
    31861 tcgaatacga tcgatgagat tgaaatccgt tgaagtcaac agaccgatgt tttgcgaatt
    31921 ctgcccgtag atcttctcga tactctgcgc gatgacgagc tgaatgcccg cagctttttc
    31981 ggcgtacggc gcggtctcgc gcgacgagcc gcagcctttt gaaagccccg agacgacgac
    32041 tgcgaagccg ccgttcttca cttcgtcttt cttcacggcg ccttcgcgca tgccgacgta
    32101 aacgtattgc ccgagcgttt cgtcgtagta gaaacacacc catccgggcg tgatctcgtc
    32161 agtcgagatg ttgctcatca acggcaggac gcccgcgctc agacgcgagg cgagctcggt
    32221 ttcgagggcg gcggcttcgt cgcccgtagc ttcgagttgc cggcggataa gtgatgtatc
    32281 gtcggttaga aatagaatgc ggcccgagaa tttgatatgg tcagggcgtc tttcggtcat
    32341 cggtttaact ggcgtctccc tgggtctgaa attcttatca tcctgtgata ttttctggga
    32401 aagcactctg attataacct cagcatgata aaacccttag tgggcccgaa agcgtctaag
    32461 cccacgtgtt gccgataaag gagcggagga gagtggatat gaagttggga caaagattga
    32521 gcctggcggt aatgctggca ttttcagtgc tgacactcgg cgctcgccag acagtgctgg
    32581 cgcaggagac ggacgatccc gtcaagatcg agatctacaa acggtttgtc gataaccgtg
    32641 cggaccgtcc ggcagtggct taccaggccg cgcgcgacta tcttcagaag tacaacaaag
    32701 acaaagatca atacacggat tacttaaaca agtgggtcat gttctacgaa cgtgacgagc
    32761 gtaagcgcaa cctcccgaat ttgatcaacg agaagaagtt tgcggaagct tacagcacgg
    32821 gcgcgaagat cctcgccgat gagccaaact acctgcgcgc gcagatcgat ctcggttacg
    32881 ccggttacct cgctgcgacc gctaaaaacg agagcttcaa cacgaccgct ctcgaatatg
    32941 cgcggaaggc gattagcgag atcgagtcgg gtaaaactcc gaccgagtgg gcgccgttca
    33001 aaggcaaaga cgacacgctc gcttacttga actacgccgt cggttacctg acgctgaaga
    33061 ccactcccga ccagtcgatc gatgcattga taaaagccgc acagtacgat agcgacatca
    33121 aaaagacggc ttcgacctat tacttcctgg ccgttgctta tgaatccggt ctctacaagc
    33181 cgctgtcgac ggactatcaa gctaaatacg cgaacaagcc cgagacgccg gaaagcaaag
    33241 ccgcgctcga gaagctaaac gtcgtgatgg atcgcatcat cgacgcttac gcgcgtgcga
    33301 ttgcgacagc gggtacggat ccaaagacgg agcagggtcg caaagattgg ctggcggcga
    33361 tgacaaacta ttacaagttt cgtcatggcg ggactgacac cgggatgact caattcatcg
    33421 cgtcggtgtt gcagacgcca ttaccgccga agccatagaa tgtctagaat caagtggacc
    33481 gatcgccgct tcagttttga ttttcctgcc ggcatttatc cggagatgat cgaacggatc
    33541 cgcgggacgc cggcacgtct cgaggatctg ttcaccggtc ttccagccga aacattaacc
    33601 gctcaattcg atggccgctg gtccatgcag gagaatgcgg gccacctgct cgatctggag
    33661 agccttgtct cgcaacgcat tgacgagtat ctggcaggca attcagcgct gcacgccgct
    33721 gacatgtcga atcgcaagac ctacgacgct aatcacaacg aggtcgctgc cgactcaatc
    33781 ctgagagcat ttcgcgctgc gaggcacgag atcgtcaacc gcctcgagag tctcgacgct
    33841 gaggttttcg aacgttctgc gcttcacccg cgactaaacg tcccgatgcg cctcgtggat
    33901 atgttatttt tccaggcgga acacgacgac taccatctcg cccgaatcag tgagattaaa
    33961 aaagctcagt gagattaaaa aaatcaaatg tctagaatcg tgctagtcat cgcagccgta
    34021 gttctgcttg ccccgaccat caacgcccaa caactcgatg caatgatcga tcgcgatatc
    34081 gcgtcgtggc tgacgacgta ccgcaatctc cacgcagcgc ccgagctatc gcatcgcgag
    34141 gaaaagactt ctgccttcgt cgcgtccgag ctgcgcaaac tcggtttcac cgtcaccgag
    34201 cgcatcggta agtatgagaa ttcgcagtgg acgggcttcg gcgtcgtggc agtgatgaag
    34261 aatggaccag gcccgacagt cctcgtcaga acagagctcg acgccttacc cgtcgaagaa
    34321 aaaacaggac taccttattc gagcagcgtg aagacgaaga acgacgcggg tgtcgacgtg
    34381 agtgtgatgc acgcgtgtgg tcacgacata cacatgacca gctttctcgg cacggcgaag
    34441 atgttgagcg agttgaagag tcgctggagt ggaacgctcg tgatgctggg ccaaccagcc
    34501 gaagaaaccg gcgacggcgc gaatgcgatg ttgcgcgaca acctctacac caatttcccg
    34561 aaacccgatt tcgcgatcgc gctgcacgat aaacccgagc tcgaaaccgg caaggtgggc
    34621 tacacgccgg gttatgcgat ggcgagtgcg acttcgatcg acgtcaagat tcgaggtgtc
    34681 ggcggtcacg gttccgcacc cgagacgacg aaggatccca tcgtggttgc ggcgcaggtt
    34741 gtcatggcct tgcagacgat cgtgagccgc gagaattcgc cgctcgatcc ggcagtggtc
    34801 acagtcggct cgattcacgg cggcacgcgt tacaacatca ttcccgatga agtgaacctg
    34861 caattgactg tacgcactta caaggaagag gtgcgcaagc gcgtgttggc gtcaatcgag
    34921 cggatcgtga aaggcgtggc ggcgaccgcg ggcattcccg aggatcgtgc gcccatagta
    34981 aaagtctctg aaggaacggg ctcgacgtac aacgatccga agttgattga gcggctcgtg
    35041 ggtacgttca agcaggcgct cggcgatgag aacgtggtgc agatgccgcc gatgatggcc
    35101 agtgaggatt tcggttattt cagcctggac cacagcattc cggcgacgat catctggctc
    35161 ggcgcatccg atccggcgaa ggtgaaggcg agccgcgaat caggtgtcgc ccttcccggc
    35221 ctccattccg ccctcttcgc gcctgttccc gaacctactc tgagaaccgg cgtcaaagcc
    35281 accacctccg ccgtcctcga actgatgaaa aaataatccg cgttcatccg cgtaaatccg
    35341 cggctaaaac ctctcgggca acccaggttc tcggcgcacc ctcaaaagct caacactcgc
    35401 ctggaggtcc cgatgaactc gacgttgcgc ttcctgtcgt tgctcctgct gttactggtt
    35461 ttcacgcaaa cttcccgcgc cactgagcct actgaagtac tggcccgcaa ggccgtttcc
    35521 gaaaacgccg ccgcatcatc ggccgcaatc gaggagttgc ggtcgctcgg ccccgccggt
    35581 ttgcaggccc tgatgactca gtacgccggc gcaattaaca cccacataaa aaacccgtca
    35641 gtcgcgccgg acgaggaatg gcaacgaatc tcggcagcgc tcgatgccgt cgcgcagcag
    35701 cggaacagct acatcgccgg gctttactgg tacacggatc tcgacagtgc gaagaaggct
    35761 gcgaaggcgc tcggcaagcc gattctctcg ttacgccttt tagggaagtt gacggacgag
    35821 tttagctgcg ccaacagtcg cttcttccgc accgtgctct atccgaatga agaagtatcg
    35881 tctgttttgc acgatcgttt cgtgctgcac tggcaatctg tgcgaccagt gccgacggtc
    35941 acgatcgact tcggcgatgg ccgcaagctc gagcgaacac ttaccggcaa cagcattcac
    36001 tacatcctcg actccgacgc gcgcccgctc gacgcgctgc ccggtttgta tggtccaaag
    36061 gcatttgttc gcggcttgat cgacaccgaa aaactcttca agtcgcttgc gggcaaagac
    36121 gacgcccaaa gaaatctgac gctgcggatc tattacgagg agcagcacaa caagatctct
    36181 gccgcatggc tgagcgacac gacgaagatc ggcggcaagg cgcctaaagg tttcatgatc
    36241 gtaaaaggca gaaacggtga tgcgttgagt atcgcgccac tcgcggtgac aaaagcgatc
    36301 actgaaacat cgatcctgcg tgcgatgaac ttcgcgacag acgagctcgg caagatcact
    36361 gatgaggccg cgtggaagaa aattgcgcag cttcatccaa ccgatgcgga gcttgacgat
    36421 cgcagcatct cgttgatccg gcgccagaac ccgacgatca cagaagacga tctcacgcac
    36481 ctcacgcgaa agtttcagga gctggtcgcg ctcgatactg tccgcaacga gtacctgatg
    36541 cacacgaagc tgtacgtgtg gttgctgaac gatccggttc gcaatgacgt cgagaagctg
    36601 aacacgaagg tttacgattc attattcctg actccaggtt ccgatccgtg gctcgggctc
    36661 ttctcacctg aggtctacac cgcgctcgac aatggtggag tgaaaaagaa ttagccgcgg
    36721 atgaacgcag atgaacgccg attcagataa ataatgtctg atccgcgttc atctgcgttt
    36781 atctgcggct ataatctccg tccatgttgc gcagcttatt gcttttgacc cttctcccca
    36841 tctcggtttt cacgcaaacg caggacaaag tgcgtgagta tcaccgcgcg aacgagcgtc
    36901 agatcttaaa agagttcacc acgctgctcg cgatcccgaa cgtcgcttct gacacgcaga
    36961 acatacgccg caacgccgcg ctgatcgtcg agatgatgaa gcaacggggc ttgaacccgc
    37021 gtctgctcga aggcccgaca ccaaacacgc caccggcagt ttacggcgaa tggaacactc
    37081 ccggcgccca acggaccatc ctcgtttacg cacattacga cggccagcca accgatccca
    37141 aacaatggac cgaaacagcg ccgtgggaac ctgtcttccg ctcggggcgc atctacgcgc
    37201 gttccgcgtc ggacgacaag gcgggcgtga tggcgattct caccgcgttc agtgcgctga
    37261 aagataaagg cgtcgcgctg acttcaaaca tcaagttctt ttttgaaggt gaggaagagg
    37321 ccgggtcacc gcatctcgga gaaatcatcg accgcaacaa agaactgctc gccgctgatg
    37381 cctggatcat cggcgatgga ccagtacacc agtcgggacg caagcaggtt gtgtttggcg
    37441 cgcgtggcga tacgaacgtc gatgtcacgg tttacgccgc gaagcgcccg ttgcatagcg
    37501 gccattacgg caactggtcg cccaacccgg ccaacctgct ggcgcgtttg ctcgcgtcga
    37561 tgaaagacga cgatggacgt gtgacgatcg agggttggta ctccgatgct gagccgctcg
    37621 gtgaagcgga gcgccgcgcg attgccgaag cgccgcaata cgatgaggag ctgaagggcc
    37681 agctcggtat caaacgcacg gaaggcggcg gcaagtcgct gctcgagttg atcaacctgc
    37741 cgtctttgaa catcaatggt tttgcaagtg gtgatgttgg cgccctcgct cgtaacgtca
    37801 tccccaccac agccacagcc gtactggatc tacgactcgt gaagggtaac gattatcagc
    37861 gccaggtgca gcggctcgtg gaccacatcc gcaagcgggg ttatcacgtt acggatcgcg
    37921 atccaactga cgctgaaagg gcacaatatc cattgctggc gcgcgtcaac gttcgacccg
    37981 gaggctataa cgccgagcgc acgcgcatgg atctgccgat ctcgaaagcc gttatcgatg
    38041 cggtgcagtc gacctctgct gagaagatcg tgttgttgtc gacgtcgggc ggaagcctgc
    38101 cgctctccat catcactgag cgactcaaga ccgtgacgat ctccgtcccg atcgcaaact
    38161 acgacaacaa ccagcacgcc gagaatgaga acatccggtt acagaacttc tgggacggga
    38221 tcgagatctg ggccgcgata atgaccatga aaaattagcc acaaaaaggc acaaaaggca
    38281 caaaatagaa acccattcaa gggaaaccat tttgtgcttt ttgtgccttt ttgtggctaa
    38341 ctttcgtggc taccaggcgc agttgtaatt cttcatcgcg gccatcgttg tttcgcgttg
    38401 cgccttgcag tgagcggcga gggtggcgcg catttgcgga tcgtcagcca ttttcttcca
    38461 gtctgtgcgc aaccgggcta ccgaggctct cacgttcgcg cgagcacctt cctcgacctt
    38521 gctgttaaca cacgtctcgt acgcggcaat gaagttgtcg cactcagcga taccaacctt
    38581 ttcaccttcg ctaaccgttg acggcgcctg agcattgcgg ttcgtcggcg ctccacaggc
    38641 gagtacgagc agaccaatca gaaaacatgc gattaaaaga aagaatttag acattttggt
    38701 cctccttttg tgaaaaagag ttgtaatata cactcattca aatctctctc cctagacgcc
    38761 cgatcgttaa ggagattacc ccaatgaaga acaaagcatt cccagctctg cttttgtcat
    38821 tctgtgtgct tttaccggcg ttggcccaga ccaagcctac cgctccacag caacctcaaa
    38881 aaccagccga cgatcaggac gaagtcgtca agatcaccac taacctcgtc caggtcgacg
    38941 cggttgtcac caaagacgga aagctggtga cggatctcac cgcggacgat ttcgagatct
    39001 acgaagacgg caagaaacag gcgattacga gttttgcgta catctcaaac gttcccggcg
    39061 ccgcgctcac cgcaactaaa gaaaagcccg gaaacggtcc acaaccaccg cgcccgatca
    39121 atcccaatga cccacatcgc acgatcgcct tcgtcgtaga cgatctcggg atttcagcgg
    39181 aaagcatgag ccaggtgcgg aaacagttgc gcaagtttgt cgacgagcaa ctacaaccga
    39241 acgacctcgt ggccatcatt cgcaccggtg gagaaatggg cgcgctccag cagttcacga
    39301 acgacaagcg actgctaagt cgcgcggcgg accaattgcg ctggaatatg tgtaatcgcg
    39361 tcggcattac ggcgttacct gtccttggct cacccagcag ccaggggtta tgcgggcgtt
    39421 actcgatgat ctcaacgatt aatgctctcc ggttcatcat cgatgcaatg ggctatctcc
    39481 ccggtcgcaa gtcaatggtg ttcatgtcgg accacttgcc ggtagaggac caggacgacc
    39541 tcgagttcct tcgcgatatt cgcagcgggg gtaacgatgg cgtaaaggac aatgcgaaca
    39601 aagcggctgc agcacgcgcg agtgatgaag ccgctgattc ggaccttggc ttcaatgacg
    39661 ccagaactta ttacgccgca ctgcgaaaga tcgccgagaa ggcgattcgc tcgtccgtgg
    39721 tgatttattc ggtcgatacg cagggtttac taacgacctc actcagtgcg gcggacaggt
    39781 tctatggcga tgggccacaa ttcaaggacg tactcagaca gcgcaatttt ttgatttgga
    39841 atcgtcgaga aggaagtgat ctgatagcca aacaaactgg cggcttccag attaggaact
    39901 caaacagctt tgagctcgat cgcatcgtgc aggaccagag tggttattac ctccttggtt
    39961 atcgtccgac agaggaaacc ttcaaccgca agttccatca catcaaggcg aaggtgaagc
    40021 gatccggaat gagcttgaga acgcggctcg gattttatgg cgtcagtgag gacgaatcga
    40081 gcaagacgcg gctcaccagg cgtgacgtga cgaacctggc gctggcctcg ccctttgccg
    40141 ctcaggacgt tgaaattcac ctggcctctt ttttcttcaa cgacaagact tacggatcgc
    40201 tcattagatc tttcttgtat atcactgcca gtgatcttga attcaccacc gttaacgggc
    40261 gacatcaagc gtcggttgag gtgcatggag tcgtcttcgg cgataacggc gcgatcgttg
    40321 agcaactccg gcgtggctta acaatcagtt tgcccgaaaa cgaatacgtg gctgcattga
    40381 aggacgggct cggattcaat gtcgacatcc ccgtgaagcg aagtggtgct ttccagctgc
    40441 gcgttgcgat cagagacagg gaaacgcaga aaatcggatc ttccggcgag ttcgtcaccg
    40501 tgcctgattt gaagaacaag aaaccggcgg tgtcaggaat tgttttaggc ggtggctctc
    40561 gtggtcgttc tgagcaggag gcactggtta acaccgtcgc ccggcggttt gacctgaaca
    40621 cggaccttga ttttgtttac gctatttaca acgcgttgca gtttgcgaag ccggtgatgg
    40681 aaacgaaact gtttcgcgac ggcaaggttg tttattcagc tccggaagtg ccgattcaaa
    40741 cagccggcca gccggatccg gagcgaatgc tggtgaatgg caaagtaccg ttgagtcgcg
    40801 acctcgagcc cggattttat tacttgcaag ttgtaatcac agataaagat gcaaagagca
    40861 aggctctgcc gcttgtgcag tggattgact tcgaggtagt caaataacga gtaaacgggg
    40921 gaggaaaatg agagtaacga tcagtttgtt ggtgttgatg tgtctgtgct cgatgacggc
    40981 ttgtgcacag gacatgaacg ggaaccacaa tcacggcgcg cctgccgctg cgtcgccggc
    41041 gtcgactgat aacatggtgt cgcaggagtg ggccaggcag cgggttgaaa aatcgccgcg
    41101 acatcgcgag tgggtgaaag tgaagaacgg cacgcgcgag gtgaatagct tcgtggtcta
    41161 tcccgagaac aaaaacaaag cgacggccgt gattgtgatc catgaaattt ttggcatgag
    41221 cgactgggtg cagagtttga ccgatcaact cgccgaagcg ggttacgtgg cgatcgcgcc
    41281 cgatctgctt tcgggaatgg ggccgaatgg cggcgggacc agctcgctca gtgggaatgc
    41341 agtgggccag gcgattcagg ctcttccgcc ggatcagatc actggtgact tgaatgcggt
    41401 ggcggattac atctcgaagg ttccggcttc gaatggcaag atcgtggtta cgggttattg
    41461 ctggggtggc ggacagtcgt tccgctatgc gacgaacaat cccaacatca aagcggcgtt
    41521 tgtcttttat ggtccagcgc cggcgtctgc tgacggcact cctgacaagg cggcgctcgc
    41581 taagataaag gcgccggtct acgggttcta cgccggcaat gacgcacgca tcaacgcaac
    41641 tctgccgaag acgattgagg cgatgacgga gttgaagaag aactatgagc cggtgactta
    41701 tgaaggcgcg ggccacggct tcatgcgagc cggagaagca cccgacgcca acgaggctaa
    41761 caagaaagca cgcgatgatg cgtgggcgcg gtggaagaaa ttactggcag caatttagcc
    41821 acggattacc acggatgaac accggatcga tccgtgttca tccgtgttca tctgtggctt
    41881 attttgataa gacttcccta aagaaatcaa ccgcccttgg atcgcccgat tcaccgagag
    41941 atcgaattgc ctgtttgcgg acgagctggt ttggatgtgt gcgtgcgatc ttaatcagca
    42001 gcggcactga ctcttcagct ttgcgttcac tgatcgccct cacagcctga acctgcacct
    42061 ccgcgttacc atccgtgctc tgcgccgtct gactcagaaa ctcgaagcta cgtttactaa
    42121 cacgctcgcc cagcaaccgg attgcctgtc gtcgcacgtc catcgcatca ttgccgctcg
    42181 caactgccag tagcttgtct tccacgcgcg ggctcttgat atccgaaagc gtctgcagca
    42241 ccgtgcgctt aacttccggc gcctgctctg aatcatacaa cttcagaaga tcgtcaacgg
    42301 ccgcttcacc gcgatcgctc agcacgcgaa tcgccgtctt ccgcagctca ggattcgcat
    42361 cgtttcgcgc gatctcgttc agacgcgttt gcgcgcgcgg gctcttcgtt tccgagagcg
    42421 aacgcaacgc cgcgcgtttc acctcgatgt tcgcgtcgtt ctggtagatc ttcatcaact
    42481 cttcgaccgc gtcttcacgc tgaaaatttc cgagcttacg cacggccgtc cgtcgtgatt
    42541 ctgggtctgg gtcgttcttc gccacgttta gcagaaacgt gaacgccggc tgctcgtcga
    42601 tactgttgcc gactgccgaa atcacactcc ggcgaatctc cggttccttc acgctttcat
    42661 aaagtccctg gagcgtcgcg accgttccgc gatcccggct ctggccaatc gcgtgcgcgg
    42721 cagccgtgcg cattttcttc tcttcggagt cgttgcgaac gatagacgcg agaaatgcct
    42781 gttcgccgcc gacctggccc aaccagtaaa cagaagtcga gcgaatgcgt tggttcggtg
    42841 agctgctgat gaaggttttg agcatgccgc cgacgcgcgc gtcgtcatgc aacgcgatgc
    42901 cgagcacggc gcgttcgctc aacatgtcga gcggtgtcgc ggcggcgata gcgcgcaggt
    42961 aattgagact ctcttcgttg tttgcgcggc cgagccagta aaccggatag ccgctgtact
    43021 cgcgtttgcg ctcgaggttg taaatctcca tgcgcgtgat ctgattactg gatggatcgc
    43081 gcagcaggaa gattgcgagg ttacgtgttt cgatcgtgac tccggccgcg ttcgtgccga
    43141 tgaagacggt cgtttcgccc atcgtgtgaa tggagccgtt aaaatcgcga acactcggat
    43201 cgactgcgac tccgggacgc acgtcgaaag tgtacgcaga ccagtaaggc gtttgttgac
    43261 cgcgagcgcg tgactgcgcg gcctcgagcc gggccatcag atctgcacca tcgactggag
    43321 tgaaactctg gatcgtggtc gagctggatt gtgtttgcag atcgtcggac gcggcggcga
    43381 ctgtgacagt gcccgcgcga tttccaccgg cgcaagcgat cccgataatc gcgagaaaga
    43441 ggagcattga gtggcgcatg aattcctccg gatgatttag aacaaacagg tagttgtcta
    43501 caggattttc aagattttca ggattaacat gtaaatcttg ttaatcctga aatcctgtaa
    43561 aggactgact ttacttcaac aattcttcca aaaacttcag cgcctcagga tctttgctct
    43621 cgccgagata cctgaccgct gctttgcgca attccactga cggatcgctc ttcgcaatcg
    43681 ccatcagctt gcggacggca accttctgct tcgagtctcc aaagccacgt agcagagcca
    43741 ttctgacttg catgttctgt tcagcgtcgt agagcgacac gagttgattg acggtctgct
    43801 cgtcgtcctt ctcgcctagc tgtcgaacgg catacatacg cagctcaacc ggctcgttac
    43861 cgcgcgctat atcaaacagc ttcgtaactg cacgaggatc ggtgctgtct ccaaaagtgc
    43921 gcagcagccc cattttgatt tgaacgttgg tctcgctcgt gtagagctgc aacaactcat
    43981 cggtcgtgac gcggttgtta tcgccgagac ggcgaatcgc ttcaatgcgc agctccggca
    44041 tttccgcctg gcgcgcgatt tcgaaaagct tcgcgcgggc cttcgggtcg tcacgctctg
    44101 cgagtgcgcg aatgacctgc gaacgaattt cttttgtctt gtcagcatcg taaatctgga
    44161 tcagcgtatc gagcgaagcc gcatcatcca tctcggcgat gtaacgaatc gcatattgtc
    44221 tggcagagat gtcggtgccg gtgcgcgcga tttccatcac cttcgcggta ccgcgcggtg
    44281 tgttgccttc taccaacgct cgcaaaatct gggcacgaac ttctttggtc tgatcggctt
    44341 cgtagatttt gatgagctcg tccgtaactt gatcggtctt tagctcgccc aggcgcctga
    44401 tcgctacgag acgcagtttc ggatcgggat tgctgcgcgc gatttccaaa agaatcggtg
    44461 tgactcgcgg tccaccatgc gaaccgagga tcgataaaac cgcagcctgg aaattctgac
    44521 atttggctgg attggcgcgg agtgcttcgg tggcgattcc gatcgcgcgt tcttcatccg
    44581 cctgaaacaa actctgcaac gcgagtattt tgatctcgca atcgtcacga tcgagcgccg
    44641 cgtcgaccgc atctttgcga ccgagcacaa cgagcattgc ctgcgcttcc tgccgccagc
    44701 tcgagccggg aaatttattg ataagcttga cgagcacggt agcggcttcg tcttttcgac
    44761 cctgcttctg caacgcatag gcgtaccagt aaagcgcggc atcgagatct ttgtctttgg
    44821 gataaccggt gatgaagtcg ttgaatttct cggcggctcg ctgccagttt tgtgcttcga
    44881 tgaagtcgcg gccttcgcga aagatctgca tcgaggccgt gtttgctttg gtctgcacaa
    44941 acttattgag tttcgagagt tcctgatcga aagcggaagt acgttgcgtg taaatcgaga
    45001 aaacggttac gaccgcggcg acagccaaca gcgcgagtcc ggatctgatt tgggggcgat
    45061 aattaactaa gctcttcata gtggtcgttt gtccttaaaa aatagttatc tacagattac
    45121 gcagattaca cagaatcgaa tctgcgcaat ctgtggatga aacttaatca agagcgcgtg
    45181 cgagtgcggt cgagttgacc cgcaacagcg gcacgatgtt tttgcgttcg acgcgttggc
    45241 gaattacgcg gatcgcgtct ttgtccggtt tgtccggaag gttagaaata tcgatcaaga
    45301 tcggttccag gctctcgagt agcgacgaga cctggacgtc gccggctgcg tcagcttcac
    45361 gacgcaacat catgttttgc agaacgagtt gttgggcgcg cttgcgttcg taagcgactt
    45421 cagccgtcgt gccgggttcg ttcaagcgca cgtttctaaa cgcgaccagc agcgtttcag
    45481 acttttcgaa gtggagcgcg gtcattgttt gcgtgtcggc agatcgcacg ggctcgggag
    45541 tcgatggaat cgcggcgaac tgctcaccgg gtttcggctc acgcaccaat ggtttcttcg
    45601 gcgccggtga cggtgaacga cgtggagccg tctgttcgtc acctttgttt ggtgaaggtg
    45661 atgcttctgg ttgtacggaa gcggacggca caggtgtcgg ggtttggacc gctaaatgtt
    45721 tgtcgttcgt tggtgtgttt gagctgcgat agatcaggaa accgatgatc gctgcgacga
    45781 tcacgagcgt tgcgaaagcg gcggcttgag cgccaaaccc ccatgtgaat ccacgtgccg
    45841 gtgcgcgtcg gggcgtggcc aggatcttct tgagcgcgcg gttttggact gccggttcga
    45901 gcgacgtttc gaagccggcg agctggctgc gcaggttaag aaagtcagcg cgcacttcct
    45961 ggcactcgtt acagccgacc aggtgacgct cgacttcgcg tgcttctgcg ggtgatagct
    46021 cgccgtcgat tagggatgag atcttttcag taaagctaca gttcatgatt gtcttcgtca
    46081 gtcttagaca ggattcacat gatttacagg attgacatga gagatcctgg tccagcgttc
    46141 tcgcagcgac gcacgcgcgc gaaagatgtc gacctttact ttgctctcag agacgttcag
    46201 cacttgtccg atctcgcgat agctcaactg ttcctgttcc cgcagcagca acgcggtgcg
    46261 ctggtcttcg ggcaacgtct gtaacatttc aagcacgagc gtttggcgtt cctgagtctc
    46321 gtattcctgc tccgggttgc gtgctcgcgg tctcatcgtc tcgacaactc gatcaaacag
    46381 ccggtagcgc gtgtgtgatt tgcgaaactc gttgatcgcc atgttgcgcg cgacgcggta
    46441 gagccagaag cgcgcctcgc cgaccggcat agttccccat gccgtacgat ggagttgcag
    46501 gaaactttcc tgtgccagat cctgcgccag cggcgcgcgg cctcccaata aacactccag
    46561 aaagcggcat aggctagggt agaaatcctg aaacagcatg gtgaactctt cgtcttcgct
    46621 cgccataagc tgcgctacgt ctcctaaaac acgtgagtt
//
LOCUS       DASSFQ010000085         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1675418, whole genome shotgun sequence.
ACCESSION   DASSFQ010000085 DASSFQ010000000
VERSION     DASSFQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675418"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="VFH96_02815"
     CDS             <1..1645
                     /locus_tag="VFH96_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6972925.1"
                     /translation="FPCFSAFAQGYKSEIEAPEKVSLVVKNLDGRVSVIASEEQQKKV
                     TIDAKSTGAPVAPEDVKVESKGSDIHIDVRPRGEKNRIDIVVTIPTRSKVEVEGQAGA
                     VDVIGSVESAVVKTDTGTIHADVPLDAVKFNFVWEASKPRYMSDVELPEIKEKAGGVY
                     KLSGRLGEKDVKKEDGLVALDFRTQRGVVLLNIDPERAPSDLRERPLTEAARAIVRSG
                     DSELVQAIRKVSPKMFGDYAKTLPPPEKEPELVRRAPPGRLVSAVAPQLMRFNASVTD
                     RNGRAIGGMRESDFTVWENGIERRVTNVSPANEPFNLVLLLDVSGSVEERMDFIRKAA
                     RDFLRTTSPQDRISIISFRDDIQVISDFSTDRGMLSRKLDEIDAGGGTALYDALGYVL
                     SEPLRKLRGDRTAIVVMSDGDDNKSFLPFPAILEALLESGALVYPLYVPSGLIPESSV
                     PKPEITIDPLRTRYLTLTTRAEDEGRKLATASGGIYYPIRRLEDLQKAYDDVVAQLRS
                     AYTITYASNSVSTSPRVRVRTNRDGASVRLSPVVELNTP"
     gene            1716..2987
                     /gene="xseA"
                     /locus_tag="VFH96_02820"
     CDS             1716..2987
                     /gene="xseA"
                     /locus_tag="VFH96_02820"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET6972926.1"
                     /translation="MNQPLFNALFDETERRPLTVSQLTNSIRIALESRFVSVWVEGEI
                     SNFKDHSSGHWYFTLKDENAQLRAKCFRSTNTRIRFRPANGLKVRARGKLSVYAPRGE
                     YELVVDALDPVGAGALRIAFEQLRDRLQKEGLFAKELKRPLPVFPRRVGVVTSPTGAA
                     IRDILNVISRRTRTVHVLFSPAKVQGEAAGPDIARAIRWINLYHEQALREGREADLVD
                     VLIVGRGGGSTEDLWAFNQEEVARAIRKSAIPVISAVGHETDFTIADFAADVRAATPS
                     AAAEIVAAREDQICSALNNMGRQLVRLTRFQIVNLRARVQEQALSHAFDEVKSRLAKA
                     RGRLDAATNACDAIIDTKVQDGRERLALAAASLDALSPRGVLQRGYAIAQDAAGRLVR
                     DAASVSPGDELNVRLAKGKLGTRIERIEATD"
     gene            3090..>3656
                     /locus_tag="VFH96_02825"
     CDS             3090..>3656
                     /locus_tag="VFH96_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972927.1"
                     /translation="MLGILALLVLLFVVVAAVGGYFAVRRYQSSPTYALALIFDAAQR
                     NDVAEFQKRVDDEEIAKNMTAAVSQKAAARYGQAIDSSMQQKIDSTMPSLLPRVKQTV
                     HDESLNAMKSFAASSEPRSFISIVSAVQSMMTVTTNGDSAKATGKLAGRDIELGMRRE
                     ADGWKVIDVKDDVIVQRVVDSVMKDLPAI"
BASE COUNT          808 a          967 c         1122 g          759 t
ORIGIN      
        1 attcccctgt ttttcagcat tcgcccaggg ctataaatcc gaaatcgaag cgcccgagaa
       61 ggtgtcgctc gtcgtcaaaa atctcgacgg tcgcgtgagt gtcatcgcct ctgaagagca
      121 acagaaaaaa gtgacgattg acgcgaaatc caccggggcg ccagtcgctc cggaagacgt
      181 gaaagtcgaa agcaaaggca gcgacattca tatcgacgtg cggccgcgcg gcgagaagaa
      241 tcgcatcgat atcgtcgtca cgattccgac gcgttcgaaa gtcgaagttg aaggacaggc
      301 aggcgcagtt gacgtgatcg gcagtgttga gtccgccgtc gttaagaccg ataccggaac
      361 gattcacgcc gacgtgccgc tcgacgcagt caagtttaat tttgtttggg aagccagcaa
      421 gccgcgctac atgagcgacg tcgagctgcc cgagatcaag gaaaaggcgg gcggcgttta
      481 caagctttcg ggacggctcg gcgagaaaga cgtaaagaaa gaagatgggc tggtagcgct
      541 cgatttccgc acgcagcgcg gcgttgtgct tttgaacatc gatccggaac gcgcgccaag
      601 cgatctgcgc gagcgtccgc tgactgaagc ggcgcgtgcg attgttcgta gtggcgacag
      661 tgagctcgtc caggcgatcc gcaaagtgtc gccgaagatg tttggcgact acgcgaaaac
      721 actgccgccg cctgagaaag aaccggagct cgtgagacgc gcgccgccgg gacgactcgt
      781 cagcgcggta gcgccgcagt tgatgcgctt caatgcgagt gtgactgacc gcaacggacg
      841 cgcgatcggc ggtatgcgcg agtcagactt cacggtttgg gaaaacggca tcgagcgacg
      901 tgttacgaat gtttcgcccg cgaacgagcc gttcaatctc gtgttgctgc ttgatgtgtc
      961 aggcagtgtt gaagaacgaa tggattttat tcgcaaagcc gcacgcgatt ttctgcgtac
     1021 gacgagtcct caggatcgca tctcgatcat cagtttccgt gacgacattc aggtgatttc
     1081 cgatttcagc actgaccgcg ggatgctgtc gcgcaagttg gacgagatcg atgcgggcgg
     1141 tggcacggcg ctttacgatg cgctcgggta tgttttgagt gagccgctcc ggaagctgcg
     1201 tggcgatcgc acggcgatcg tcgtgatgtc agacggcgac gacaacaaat cgtttctgcc
     1261 gtttccggcg attctggaag cgttgctcga atcgggcgcg cttgtttacc cgctgtacgt
     1321 gccttccggt ttgataccgg agtcgagcgt gccgaagccg gagatcacga tcgatccttt
     1381 gcgaacgcgc tatctcacct tgacgacgcg cgccgaggat gaaggacgca aactcgcgac
     1441 ggcgtcaggc ggtatttact atccgatccg tcggctggag gatttgcaga aagcgtacga
     1501 tgatgtagtg gcgcagctcc gctccgccta cacgatcact tacgcatcaa attctgtctc
     1561 tacatcgccg cgtgtgcgcg tgcgcaccaa tcgtgatggc gcgtctgtga gattgagtcc
     1621 ggtagtagag ttgaacacgc cgtaacaact cttatccaca gattacacag attgttttag
     1681 aacatctgtg taatctgtgt aatctgtgga cgaaaatgaa ccaacctctt ttcaacgcac
     1741 tattcgacga aacagaacgc cgcccgctaa ccgtctccca actaacaaac tcgatccgga
     1801 tcgcactcga atcacgcttc gtttccgtct gggtcgaggg cgagatatca aactttaaag
     1861 atcactcttc gggtcactgg tacttcacgc tcaaagacga aaacgcacaa ctgcgtgcga
     1921 agtgctttcg ctcaaccaac acgcggattc gttttcgtcc cgccaacggt ctgaaagttc
     1981 gcgcgcgcgg caagctctcc gtttacgcgc cacgcggcga gtatgagctc gttgtcgacg
     2041 cgctcgatcc ggtcggcgcc ggcgcattgc gcatcgcgtt cgagcagctc cgcgatcgtc
     2101 tgcaaaaaga aggtctgttt gcgaaagagc tcaagcgtcc gttaccggtg tttccgcgtc
     2161 gcgtgggcgt cgtaacttca ccgacaggcg ccgcgattcg cgacattctc aacgtgatct
     2221 cacgtcggac gagaacggtg cacgtgctct tttcccccgc taaggtgcag ggtgaggccg
     2281 ccggtcctga tatcgcacgc gcgatacgtt ggatcaatct ttatcacgag caagcactgc
     2341 gcgaaggacg cgaggcggat ctcgtcgatg ttttgatcgt cggtcggggc ggtggctcga
     2401 cagaagatct gtgggcgttt aatcaggaag aggttgcgcg cgcgattcgc aagtctgcga
     2461 ttcccgtgat ctctgctgtg ggtcacgaaa cagatttcac gatcgctgac ttcgccgcgg
     2521 acgtgcgcgc ggcaacacca tccgccgccg ccgagatcgt cgctgcgcgt gaagatcaga
     2581 tctgctcggc gctgaataac atgggccgac agctcgtacg actgacgcgt tttcagatcg
     2641 tcaacttgcg cgcgcgtgtg caggagcagg cgctgtcaca cgctttcgat gaagttaaat
     2701 cgcgtctggc aaaagcacgc ggacggctcg acgcggcgac aaacgcgtgc gatgcgatca
     2761 tcgacacaaa ggtgcaggac gggcgcgaac ggctggcgct ggcggccgca tcgcttgacg
     2821 cgttgtctcc gcgtggagta ttgcaacgag gctacgcgat agcgcaggat gctgccggta
     2881 gactggtgcg ggatgcggct tcagtgtcgc caggtgatga attgaacgtg cggttggcaa
     2941 aaggaaaact gggaacgcgg atcgaacgga tcgaagccac ggattagcgc ggatcgagcc
     3001 aaatgcggca aagaattgta attaatctcg acgggccggc ggcagaggga acaaagcctg
     3061 tcagcaggaa acgcaggcga tggccacgtg tgctcgggat tctggcgttg ctggtgttgc
     3121 tgttcgtggt ggtggcggcg gtgggtggtt atttcgcggt gcggcgttat cagtcgtcgc
     3181 cgacttacgc actcgcgctc attttcgatg ccgcgcaacg gaatgatgtc gccgagtttc
     3241 agaaacgcgt tgacgacgaa gaaatcgcaa agaatatgac tgctgccgtc agtcagaaag
     3301 cagcggcgcg ttacggccag gcaatcgata gctcgatgca gcaaaagatc gacagcacaa
     3361 tgccgtcgct gttacctcgt gtgaagcaaa cggttcatga tgaatccctg aacgcgatga
     3421 agtcgttcgc ggcttcgtcg gagccacggt cgttcatatc tatcgtcagt gcagttcagt
     3481 caatgatgac agtcactacc aacggtgatt ctgcaaaagc aaccggaaag ttggcagggc
     3541 gtgatataga actcgggatg cgacgcgagg cggatggctg gaaagtaatc gacgtgaaag
     3601 atgatgtcat cgtgcaacga gtggttgata gtgtgatgaa agatctgccg gcgatc
//
LOCUS       DASSFQ010000086         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1816565, whole genome shotgun sequence.
ACCESSION   DASSFQ010000086 DASSFQ010000000
VERSION     DASSFQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816565"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..825)
                     /locus_tag="VFH96_02830"
     CDS             complement(28..825)
                     /locus_tag="VFH96_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6972928.1"
                     /translation="MMQITSRDNSLLREARAVRDGKIDDLIFVEGLRLCEEAFRSKLE
                     IEAVIASDELLRKDRAAIAIAELSHIAKRNASVTEKLLASISYTKTPQGIVVLARRPM
                     ASENRLSESLDPNSLLVVLHQINNPVNVGAILRTAEAAGANGVITTANTSDPFSPKSL
                     RGAMGSAFRLPIWIGPTYTKTIEWCKARGISTVCADVGGAMAYTEVDWTGARALFLGP
                     ESTGFTLEELMQADKAVTIQMRGSAESLNVSVAAGILLFEAARQRQL"
     gene            complement(822..1676)
                     /locus_tag="VFH96_02835"
     CDS             complement(822..1676)
                     /locus_tag="VFH96_02835"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6972929.1"
                     /translation="MIPPKLVIASYNIRYARGPYLIWGGLRRKLGLMNLKGRPAHVGN
                     LISGAARAFSDNKLLPRVDVLALQEADKGTLRTGGHHVAKELAEQLEMYWEHAPAGIP
                     RGVKPVKRQWWLDFEEPIDLNDEGDTGVALMSRFPLSDVTRIDLPWHECPWRPRLAMA
                     ATISVGPHQLRIFNAHVDPHAAIDGQLEQLEVIAAQADATTLPAIILGDFNTLSKQKC
                     IDTRAFLESRGYATPIPTGTSTWRGGGLQMHADWIFSRGVKIERWGVARPLSVSDHWP
                     IWAEIIIA"
     gene            1675..2031
                     /locus_tag="VFH96_02840"
     CDS             1675..2031
                     /locus_tag="VFH96_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972930.1"
                     /translation="MSGVIIAVVDDMFFKSKIRAVAEAVGTEISFPRNQDALFQKARD
                     AKPQLIIVDLHNQKIEPATLAAELKADDELRAIPLLGFFSHVQTELQRNALAAGFDQV
                     IPRSVFARDLSKILSV"
     gene            complement(2043..3050)
                     /gene="aroF"
                     /locus_tag="VFH96_02845"
     CDS             complement(2043..3050)
                     /gene="aroF"
                     /locus_tag="VFH96_02845"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6972931.1"
                     /translation="MKHDATDSQVEAVLRVIEALGFKGHPMPGATRTAIGITGNQGAV
                     DPSHFENLAGVADAIRVSKPYKLISLDLRPEKTIVRVGDATIGGDELAIIAGPCAIES
                     RAQAFAIAEAVRRSGARFFRGGAYKPRTSPYAFQGLGEEGLKILADIREAYGLKIVTE
                     ALDEAGVDLVERYGDMIQIGARNMQNFSLLRRAGKAKLPVLLKRGLSATLDEWLLAAE
                     YVMAEGNYQIVLCERGIRTFAQHTRNTMDLAAIPAVRRISHLPVIVDPSHGTGKNFMV
                     TPLARAGVAVGADGLIVEVHDQPEHALSDGAQALTLDQYDQLVSEVRSIYEVIAPAAV
                     G"
     gene            complement(3182..>3407)
                     /locus_tag="VFH96_02850"
     CDS             complement(3182..>3407)
                     /locus_tag="VFH96_02850"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HET6972932.1"
                     /translation="DEVIVQCRNSAEPANLAKYTFSLARSFSRFYHDHRILTEQDEAR
                     RGVLIAVTKIIRTQLTTALGILGINVPEQM"
BASE COUNT          751 a          979 c          906 g          771 t
ORIGIN      
        1 accgcactcc aaggaggcac ccaaacctca taactgtctt tgacgagccg cttcgaacag
       61 gagaataccg gcggccacag agacgttcag actttcagca ctccctcgca tctggattgt
      121 caccgcttta tcggcctgca ttaattcctc gagcgtgaag ccggtcgatt ccggccccag
      181 aaagagagcg cgggctccgg tccaatccac ctcagtgtag gccatcgccc ctccgacatc
      241 agcacaaaca gtcgagatgc cacgggcttt acaccattcg atcgtctttg tgtaagtcgg
      301 tccgatccaa atcggtaatc taaacgccga ccccatcgca ccacgcagag actttggcga
      361 gaatggatcg ctagtgttcg cagtcgtaat aacgccgttc gcgccagcgg cctcggcggt
      421 gcgcagaata gcgccgacat taaccggatt gttgatttga tgaagcacta ccagcagcga
      481 attcggatct aggctctcag agagacgatt ctctgaagcc atcggtctac gggcgagaac
      541 cacgatgcct tgcggtgtct tcgtgtaaga gatcgaagca agcagttttt cagtgaccga
      601 agcgttgcgt ttcgcgatgt gcgatagttc agcaatcgcg atggctgcgc gatctttgcg
      661 cagaagctca tccgatgcga ttaccgcctc gatttcgagc ttcgagcgaa atgcttcttc
      721 acacagccgc aacccttcga caaaaatcag atcgtcgatc ttcccatcgc gaaccgctcg
      781 ggcttcgcga agaagggaat tgtcgcggct ggtgatctgc atcatgcgat tatgatttca
      841 gcccagatgg gccaatggtc ggaaacactc aaaggtcgcg ctacacccca gcgctcgatc
      901 tttactccac ggctgaagat ccaatcggcg tgcatttgca gtccaccgcc tcgccacgtc
      961 gaggtgcctg tcggtatcgg cgttgcatag ccgcgtgact ccaggaacgc gcgcgtgtcg
     1021 atgcatttct gttttgagag cgtgttgaag tcgccgagga tgatggcagg caatgtggtc
     1081 gcgtcggcct gtgccgcaat tacttcgagc tgctcgagtt ggccatcgat tgcagcatgc
     1141 ggatcgacat gcgcgttgaa gattctgagc tggtgtggcc caaccgagat cgtggctgcc
     1201 atcgcgagtc gtggtcgcca tgggcactcg tgccacggca agtcgattcg cgtcacgtcg
     1261 ctgagaggaa aacgactcat aagcgcaacg cctgtgtcgc cctcatcgtt tagatcaata
     1321 ggttcttcaa aatcgagcca ccattgccgt ttcaccggct taactccacg cggaattcct
     1381 gccggagcgt gctcccaata catttccaat tgttcggcca gctcttttgc gacgtgatgt
     1441 ccgcccgtgc gtagagttcc tttatctgct tcttgcaacg ccagcacatc gacacgcggt
     1501 aacaacttgt tatcggagaa ggcgcgtgcg gcgccagaga tcagattacc gacgtgtgcg
     1561 ggccgtcctt ttaagttcat caatccgagt ttgcgtcgca gtccgcccca gatcaggtat
     1621 gggccacgtg cataacgaat gttgtatgat gcgatgacaa gcttaggagg aatcatgagc
     1681 ggcgtgatta tagcagttgt cgacgatatg ttttttaagt cgaagatccg cgcggtggcg
     1741 gaggcggtgg gaactgaaat cagttttccg cgaaatcagg atgcgttgtt tcagaaagcg
     1801 cgcgatgcaa agccacaact aataatcgtc gatttacaca accaaaagat cgaacccgcg
     1861 accctggctg ccgagctgaa ggccgacgac gaactccgcg ctatcccgtt gctcggcttc
     1921 ttctctcacg tccaaacaga actacagcga aacgcacttg ccgccggctt cgatcaggta
     1981 atcccgagat ccgtgttcgc tagagatcta tcgaaaatac tatccgtgta atccgtggct
     2041 aactaaccaa ctgccgctgg cgcgattacc tcgtaaattg acctcacttc gctcaccaat
     2101 tgatcgtatt ggtccaacgt cagcgcctgc gcgccatccg agagcgcatg ctctggctga
     2161 tcgtgaacct cgacgattag tccatccgca ccaaccgcaa caccagcgcg cgcgagcggc
     2221 gtaaccataa aattctttcc ggttccatgt gacggatcaa cgatcacggg caggtgtgaa
     2281 atgcgtcgca cggccgggat cgcagccagg tccatcgtgt ttcgtgtgtg ttgagcaaac
     2341 gtgcggatgc cgcgttcaca gagcacgatc tgatagtttc cttcagccat cacatactct
     2401 gccgccagca accactcgtc gagcgtggcc gatagcccgc gcttgagcag caccggcaac
     2461 ttcgccttgc ctgcccgtcg cagcaaagag aagttttgca tgttgcgtgc gccaatctga
     2521 atcatgtcgc cgtagcgttc gacgagatcc acgcccgctt cgtcgagcgc ttccgttacg
     2581 atcttcaaac cgtaagcttc acgaatgtcg gcgagtatct tcaatccctc ttcgccgaga
     2641 ccctggaaag cgtaaggcga cgtgcgcggc ttgtaagcgc cgccacggaa aaagcgcgca
     2701 ccactgcgtc gcactgcttc ggcaatcgca aaagcctggg cgcgactctc aatcgcgcat
     2761 ggaccagcga tgatcgccag ttcgtcaccg ccaatcgtcg cgtcgccgac gcgcacgatc
     2821 gtcttttccg gtcgcaggtc gagactgatt agcttgtaag gcttgctgac gcgaatcgca
     2881 tcggcaacgc cggcaaggtt ttcaaagtgt gacggatcga cggcgccctg gtttccggtg
     2941 ataccgatgg ctgtacgcgt cgcgcccggc atcggatggc ccttgaagcc caatgcctcg
     3001 atcacgcgca ggacagcttc gacctggctg tcagtggcgt cgtgtttcat tacgattaac
     3061 atggtagttg gtccttaatg aagaattctc gcgcaaaggc gcaaaggaaa gcagcaaaga
     3121 agtggttagg tttctttgcg ttctcctttg cgcctttgcg cctttgcgca agaattcttc
     3181 tttacatctg ctcgggtaca ttgattccaa gaattcctaa agctgtagtc aactgagttc
     3241 tgatgatctt cgtcactgca atcagcacgc ctcgtcgcgc ttcatcctgc tccgtaagaa
     3301 ttcgatgatc gtgatagaag cggctaaaag agcgggcaag cgagaatgtg tacttcgcaa
     3361 gattagcagg ctctgcggaa tttcgacact gcacaatgac ttcatcc
//
LOCUS       DASSFQ010000087         7023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_336400, whole genome shotgun sequence.
ACCESSION   DASSFQ010000087 DASSFQ010000000
VERSION     DASSFQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336400"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFH96_02855"
     CDS             complement(<1..499)
                     /locus_tag="VFH96_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET6972933.1"
                     /translation="MSSIKRIGIVLKPHQPEALKTICELVVWLAERDIKLIGGPEIER
                     EQIEHETGCPVDRSETERLAEDVDLMLVLGGDGTMISTARMIGDQEVPVLGVNYGGLG
                     YLAEFRIEELYTALESILSGNYRLDRRVMLEVQLNRAGEASKTSRVLNDVVINKSALA
                     RIIEIE"
     gene            complement(598..1338)
                     /locus_tag="VFH96_02860"
     CDS             complement(598..1338)
                     /locus_tag="VFH96_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET6972934.1"
                     /translation="MTRERIDKLLVERGLAESRTKAQAMVMAGIVLVDEQRVEKPSHQ
                     FETNSTIRIKGGDDPTTRYVGRGGLKLEAALREFQINVSGLVCLDVGASTGGFTDCLL
                     QHGATEVFAVDVGHNQIDWRLRNEPRVEVREGVNARYLTPEDFPNKFDLVVMDVSFIS
                     VTKVLPAIVPLLATGGSIVTLIKPQFEVGRGEVGGGGIVRDETKRLRVVEEVNSAARE
                     LGLDVEQIIESPITGAEGNVEYLVLYRV"
     gene            complement(1366..1617)
                     /locus_tag="VFH96_02865"
     CDS             complement(1366..1617)
                     /locus_tag="VFH96_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972935.1"
                     /translation="MTRQMYRERECIHREDGADGDFYNGVFYIQALQRLRVNEAVQMA
                     SKVTSFFWADAPHILVWLCDDCAAELRLTETPRAMTRRA"
     gene            complement(1782..2375)
                     /locus_tag="VFH96_02870"
     CDS             complement(1782..2375)
                     /locus_tag="VFH96_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972936.1"
                     /translation="MKDRFEPNFDDERTLLAAQQVVPLEKINAKVRHRKQWFLGGAFA
                     VAMMLGAASALLASYFKMRNVQPVPVEVTQEITPVEEPAATVAAVEEPEEAVEEPKEE
                     VIPEESTPKKEAPVKRRTVVAAAKPNDSISEPSDTRQLGEEDQLHQIRDAVLYDEWQE
                     RRARRVWRRERRRAERYNHRDLSNLDEIFEGRRRPNP"
     gene            complement(2525..4444)
                     /gene="dxs"
                     /locus_tag="VFH96_02875"
     CDS             complement(2525..4444)
                     /gene="dxs"
                     /locus_tag="VFH96_02875"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET6972937.1"
                     /translation="MTLLESINSPADLRTLSPEQLRTVADEIRAYILETMSRVGGHTG
                     ASLGAVELAVALHYAFDTPRDRLVWDVGHQAYAHKILTGRRDLLPTIKQYGGISGFLR
                     RDESEYDTFGAGHASTSLSAALGMAIARDRKGEDHHVVALIGDASLAGGMAMEAINQA
                     GHLKSRLIVVLNDNEMSIAPAVGALTGYLNRIRGAHGYHRFKDEVEEKLLAIPSVGER
                     LHHAAKTMKDVIAAAVLPGALVSELGFKYIGYVDGHNPRALVAALKEAKQIKDGPVIV
                     HALTTKGKGFPQTEPDYYRWHATGPFDLKTGKPQSSSSKTPTYTKVFGDTLAHLMEKD
                     PKVVALTAAMPDGTGICTPLEKFPERSFDVGIAEQHCVTFAAGMSCEGLKPVCAIYST
                     FLQRGFDQLVHDVCIQDLNVKFCLDRGGVAGGDGQTHHGLLDIAYLRPIPNIVLMAPK
                     DEGEMRDMLFTMLEHTGPAAMRYPRGNGVGVNIDREPELLEIGKGEIMRDGGEVAIVA
                     YGAMVHPSLAAADRLAKDRIETTVVNARFVKPLDTPLLLALARTKRVIVTVEEAYLAG
                     GFGSAVMELLEENGLQDKVHVVRMGIPDRLITHGDAKLLLAKYGLDADGIYNRVKETI
                     DVLDDRRTKARRTVS"
     gene            complement(4674..5006)
                     /locus_tag="VFH96_02880"
     CDS             complement(4674..5006)
                     /locus_tag="VFH96_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972938.1"
                     /translation="MQRRDVVEACFVTLHLWFIRQPRAQFAEDSGNVCVGWLREAIVG
                     PFAIATSGDESGAAQVGEMTRNLWLISLQNLDTGANAQLIIAQKMNQPQPSVVREGFE
                     ECFQISMH"
     gene            complement(4993..5832)
                     /locus_tag="VFH96_02885"
     CDS             complement(4993..5832)
                     /locus_tag="VFH96_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6972939.1"
                     /translation="MPSRRQFLKGLVAAPLVGLSATAAYSRLIEPYNYLVSETDIFVR
                     DLPAAFEGFRITQLTDVHHSRILGLEVVQRVVNLAQQTKPDMFVLTGDYSTTYRRYIE
                     PCAEALGALSAPEGVWAVLGNHDHYTDPELTTRALQRHHVAVLNNAHTTLQRGSDAIQ
                     LSGIDDFTWNATDWTRAFSGLKTGTPTILLSHQPTVLDFEQTKDVSLIVSGHTHGGQL
                     DFPLLGAPARFATPDLKYARGLFRRGDTQLYVSAGTGVIGLPLRFGVRPEIAVLRLRR
                     AAT"
     gene            5917..6007
                     /locus_tag="VFH96_02890"
     tRNA            5917..6007
                     /locus_tag="VFH96_02890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5951..5953,aa:Ser,seq:gct)
     gene            6070..>7023
                     /gene="gltX"
                     /locus_tag="VFH96_02895"
     CDS             6070..>7023
                     /gene="gltX"
                     /locus_tag="VFH96_02895"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET6972940.1"
                     /translation="MTVRVRFAPSPTGYLHIGAARSALFNWLYARKTGGKFLLRIEDT
                     DLQRSTDESTRSIIEGLEWLGLDYDEDLVFQSTNAPAHRAAANRLLEEGKAYRDFTPK
                     EQRSDNNVKEEIAERARTQGLEGVDQRSNPYRDLSKEESDRRAAAGESFAIRLKVPNE
                     GQTRFDDIVYGTQERSYAEIEDLVLLRSDGHPLYNLSVVIDDITMGITHVIRGQDHLT
                     NTHKQILLYQALGASVPQFAHLPLILAPNKGKLSKRKHGEVVSLTTYRDRGFVPAAFR
                     NFLALLGWAPPEGKEVLSKEELVEYFSLEGIGRANAIFNFQE"
BASE COUNT         1593 a         1989 c         1847 g         1594 t
ORIGIN      
        1 cttcgatttc gatgatgcgg gcaagagctg acttattaat cactacgtcg ttcagcacac
       61 ggctggtctt cgacgcctcg ccggcacggt ttaattggac ctcgagcatc acgcgtctat
      121 cgagacggta gtttccggac aggatcgact cgagcgccgt gtacaactct tcaatacgaa
      181 actcagcgag ataaccaagg ccaccgtagt taacgccgag aacaggaact tcctgatcgc
      241 caatcatgcg ggcggtggag atcatggtgc cgtcgccgcc aagcaccagc atcaaatcga
      301 cgtcttctgc gagtcgttca gtttcgcttc gatcgactgg gcagccggtt tcgtgttcga
      361 tctgctcgcg ctcgatctcc ggtccaccga tgagcttgat gtcgcgctcg gccagccaca
      421 ccaccagctc gcagatggtt ttcagggctt caggttgatg cggtttaagg acgatgccta
      481 tccgcttgat tgacgacatg gtttgcgcgg gaagcgtaaa gcatcctcac gcaaaggcgc
      541 aaaggaaaaa gaggtatttt caactgtact tagccgtctt tgcggctttg cgtgaaacta
      601 aactctgtaa agaaccaaat actcgacgtt gccttctgcg ccggtgatgg gcgattcgat
      661 tatttgctca acgtcgaggc caagctcacg tgcagcacta tttacttcct cgaccacccg
      721 caatcgcttc gtctcatcgc gcacgatccc gccaccacca acctcgccgc gaccaacttc
      781 aaactgcggc ttgatcagcg tcacgatcga accgcccgtc gccagcaacg gaacaatcgc
      841 cggcaacacc ttagtcacgg aaataaacga gacgtccatc acgaccagat cgaatttgtt
      901 tggaaagtct tcaggcgtga gatagcgcgc gttcactcct tcacgaacct cgacacgcgg
      961 ctcgttacgc agtcgccaat cgatctggtt gtggcccaca tcgaccgcaa aaacctccgt
     1021 cgcgccgtgt tgcaacagac agtcagtgaa accaccggtc gaagcgccca cgtcgagaca
     1081 cactaacccc gagacgttga tctgaaattc acgcaacgct gcttcgagtt tcagaccgcc
     1141 acgaccgacg taccgagtag ttggatcgtc gccgccttta atacgaattg ttgagttggt
     1201 ctcgaattgg tgggaaggtt tttcaaccct ttgctcgtcg acaagcacga tcccggccat
     1261 caccattgcc tgggcttttg ttcgggattc tgcaaggcct cgctcgacga gcagcttgtc
     1321 gattcgttca cgcgtcacgc gacaagatta acaagaaatc ctgtctcaag cgcgccgcgt
     1381 catcgctcgc ggtgtttcag ttagcctgag ctctgccgcg cagtcatcac agagccaaac
     1441 gagaatgtgt ggagcatcgg cccaaaagaa tgacgtaacc ttactagcca tttgcacagc
     1501 ctcgtttacg cggagtcgtt gcaaagcctg gatgtagaac acgccattgt aaaaatcgcc
     1561 atcggcgccg tcttcgcggt gaatacactc tcgttcgcgg tacatctgtc tagtcatgat
     1621 gattcaccat atccccgcgt ccgcctgacg cgaaaaacag atccattaat caaaatgtcg
     1681 gggattgcta tgaaatagtg tgagttgaaa acagggcgaa taacgtagtg aggagatgat
     1741 gccactcgat tacgttcacg tcaatagatg tattttttgc actatgggtt aggtcttcgg
     1801 cgaccttcga agatctcgtc gagatttgaa agatcgcgat ggttgtagcg ttcagcgcgg
     1861 cgacgctcac gtctccaaac acgtcgcgca cgacgttcct gccattcgtc gtaaagcacg
     1921 gcatcgcgaa tctgatgcaa ctgatcctct tcgcccagtt ggcgagtatc gctgggctca
     1981 ctaatcgagt cgttaggctt ggcagcggcc accacggttc gtcgctttac tggtgcttcc
     2041 tttttcggag tgctctcctc gggaatcact tcttcttttg gttcttcgac cgcttcttct
     2101 ggttcttcaa ccgcagctac tgttgcagca ggctcctcaa cgggtgtgat ttcttgcgtc
     2161 acttccaccg gaacaggctg cacgtttcgc atcttgaaat acgaggctaa caacgccgac
     2221 gccgccccca gcatcatcgc gaccgcgaac gcgccgccga gaaaccactg tttgcgatgc
     2281 cgcaccttcg cgttgatctt ctccagcgga accacctgtt gcgccgccaa taacgtacgc
     2341 tcatcatcaa aatttggctc gaatctatct ttcatcactt gttcggatcc tctttgcaat
     2401 cacgaggcca aacagatcac aactattttt ggggagagat ttacgggaga ggaacggaaa
     2461 tggtgcactg atgccacaaa tcggcacaaa gccacagttt gtgcattttg tgcctttttt
     2521 gtggctaaga aactgtgcgg cgtgctttag tgcggcgatc gtcaagcacg tcgatggttt
     2581 ctttcacgcg attgtagatt ccgtcagcgt cgagaccgta tttcgccagc aacaattttg
     2641 cgtcaccatg agtgatgaga cggtccggaa ttcccatgcg caccacatgg accttgtcct
     2701 ggagaccatt ctcttccagc aattccatca ctgccgatcc gaaaccaccg gcgagatatg
     2761 cttcttcgac ggtgacgatc acacgtttgg tgcgcgcgag agcaagtaac aacggcgtgt
     2821 cgagtggctt aacaaatctc gcgttcacca ccgtggtctc gatgcgatcc ttcgcgagtc
     2881 gatcagccgc cgcaagtgac ggatggacca tcgcgccgta ggcaacaatc gcgacctcgc
     2941 caccgtctcg cattatctcg cctttgccga tttcgagtag ttccggttcg cgatcgatgt
     3001 taacgccgac gccattgcca cgcggataac gcattgccgc cggcccggta tgctccaaca
     3061 tcgtgaacag catgtcgcgc atttcgcctt cgtctttcgg cgccatcaat acaatgtttg
     3121 gaattggtcg caggtacgcg atgtcgagca gaccgtgatg cgtttgtccg tcaccacctg
     3181 cgacgccgcc acggtcgaga caaaacttca cgttgagatc ctgaatacag acgtcgtgca
     3241 ccaactgatc aaaaccacgc tggaggaaag tcgaatagat cgcgcagacg ggcttcaaac
     3301 cttcgcacga catgcccgcg gcaaaagtga cgcagtgttg ctcggcaata ccgacgtcga
     3361 aagatctttc gggaaacttc tcgagtggag tgcaaatgcc cgtgccgtcc ggcatcgccg
     3421 ccgtcaaagc gacgactttt ggatccttct ccatcaggtg tgccagcgtg tcgccaaata
     3481 ctttcgtata agtcggcgtc ttggacgagc tcgactgcgg ctttccggtc ttcagatcaa
     3541 atggtccggt ggcgtgccag cgatagtagt cgggttcggt ttgcggaaaa ccttttccct
     3601 tcgtcgtcaa agcgtgaacg ataactggtc catccttgat ctgtttcgct tctttaagag
     3661 cggcgacgag tgcgcgcggg ttgtggccgt ccacatagcc gatgtacttg aagcccagtt
     3721 cggatacgag tgcgccaggt aaaaccgcgg ctgcgatcac gtccttcatc gtcttcgctg
     3781 cgtgatgcag tcgttcacca actgatggaa tcgcgagtag cttttcttca acttcatctt
     3841 tgaagcggtg ataaccgtgc gcgccgcgga tgcgattgag ataacccgtt aacgcgccga
     3901 ctgcaggcgc gatcgacatt tcgttgtcgt tgaggacgac gatcaagcgg ctcttcagat
     3961 gtccggcctg gttgatcgct tccatcgcca tgccgcccgc gagcgaagca tctccgatta
     4021 atgcgacgac gtgatgatct tcgcctttgc gatcgcgtgc gatagccatt ccgagagctg
     4081 ccgagagaga cgttgaagcg tgtcccgcac cgaacgtgtc gtattcagac tcgtcgcggc
     4141 ggagaaaccc gctgatgcca ccgtactgtt tgattgttgg gagcagatcg cgtctgccgg
     4201 tcagaatctt gtgcgcgtag gcctggtgac cgacgtccca aacgagccga tcgcgcggcg
     4261 tgtcgaacgc gtaatgcagc gccactgcaa gctcgacggc gcctagtgaa gcgccggtat
     4321 gaccgccgac gcgcgacatc gtttcgagga tgtaagcgcg aatttcgtcg gcgacggtgc
     4381 gaagttgttc gggtgaaagc gtgcgcaggt ctgcgggtga gttaatactc tcgagaagcg
     4441 tcatcttggc cgcattgtac tccaatcgtt ggcttttgct atctcgcaca tagccgctac
     4501 ttcacggcat aaagtactct gttgtactcg aaattgtcaa tagtttcacg caaaggcgca
     4561 aagggagcaa aggctgggaa gaaagagcgg gcttaacttt gcggctttgc tctctttgcg
     4621 cctttgcgtg aaatacgaac tgaactgcct ctcaaataag atcgtaatcg gattcaatgc
     4681 atggagatct ggaaacactc ttcaaagcct tcgcggacca cactcggctg cggctgattc
     4741 atcttctggg cgatgatgag ctgtgcgttt gctcctgtgt cgaggttctg aagactaatc
     4801 agccaaagat ttcgcgtcat ctcgcctacc tgcgccgcgc cggactcgtc gccgctcgtc
     4861 gcgatggcaa atggacccac tatcgcctca cgcaaccatc cgacacacac attgccagaa
     4921 tcttcagcga actgcgcgcg cggctggcga atgaaccaca aatgcaacgt gacaaagcac
     4981 gcctcgacaa catcacgtcg ctgcacgccg taaacgcagc acagcaatct ccggacgcac
     5041 accaaaacgt agcggcaaac caataacacc cgtccccgct gacacgtaaa gctgtgtgtc
     5101 gccacgacga aataaaccac gcgcatattt cagatcaggc gttgcaaaac gtgccggcgc
     5161 accaagcaac ggaaaatcca attgtccacc gtgcgtgtgt ccactaacga tcaacgacac
     5221 gtctttagtc tgctcaaaat cgagcactgt tggttgatgc gacaataaga tcgtcggcgt
     5281 gcctgtcttc aaccctgaaa acgcacgcgt ccaatcggtt gcgttccacg taaaatcgtc
     5341 gattccagac aattgaatcg catccgatcc gcgttgaaga gttgtatgag cgttattcag
     5401 cactgcgacg tgatggcgtt gcagcgcgcg agtggttaat tccgggtcgg tgtagtgatc
     5461 atgattaccg agcacggccc aaacgccttc cggcgcactg agcgcaccga gagcttcggc
     5521 gcagggctcg atgtaacggc gataagtcgt actgtaatca ccggtgagta caaacatatc
     5581 gggtttcgtc tgttgagcca gatttactac acgttgcacc acttccaaac cgagaatgcg
     5641 actatgatgg acatcggtca gctgcgtgat gcgaaagcct tcgaaggcag ccggcaagtc
     5701 gcgaacgaag atgtctgttt cggatacgag ataattgtaa ggctcgatca accgcgaata
     5761 agccgccgtt gctgaaagtc cgacgagtgg cgcggcaacc aagcccttga gaaactgccg
     5821 gcgtgatggc atccgcgaat cttaactcaa accgaacctg gcttccttga caatccgcgt
     5881 ctcgcgacta gaattggtct tcgccgattg cggcacggag atgtggccga gtggctgaag
     5941 gcggcggttt gctaaaccgt taagggtcaa aagcccttca caggttcgaa tcctgtcatc
     6001 tccgccagat ttttaagcca caaagaagca caaaagactt gacgtgcttt tttgtggctt
     6061 acaactttta tgactgttcg ggttcgattt gctccgtctc cgacgggcta ccttcatatt
     6121 ggcgccgccc gctccgctct ctttaactgg ctctacgcgc ggaagactgg cggcaagttt
     6181 ctgctgcgca tagaagatac cgacctgcaa cgttcgactg acgaatcaac gcgctcgatt
     6241 atcgaaggac tcgagtggct cggactcgac tacgacgaag atctcgtgtt ccagtcaacc
     6301 aacgcgcccg cacatcgcgc tgccgccaac cgtttgctcg aggaaggcaa ggcgtatcgc
     6361 gacttcacgc ccaaagaaca gcgtagcgac aacaatgtaa aggaagagat cgccgagcgc
     6421 gcgcgcacac agggactcga aggtgtagac caacgcagta acccgtatcg cgacttgtcg
     6481 aaggaagaat ccgatcgacg tgctgcagca ggcgagtcgt tcgcaattcg tttgaaagtt
     6541 ccgaacgaag gccaaacgag attcgacgac atcgtttacg gaacgcagga gcgcagctac
     6601 gcagagatcg aggacctcgt gctgctacgt tctgatggtc atccgcttta caacctctcc
     6661 gtggtgatcg acgacatcac gatgggtatc acgcacgtga ttcgcggcca ggaccatctg
     6721 acgaacacac acaagcagat ccttttgtat caggcgctgg gagcttcggt gccgcagttt
     6781 gcgcacctgc cgctgatact cgcgccgaat aaaggcaagc tctccaaacg caaacacggc
     6841 gaggttgttt cgttaacgac gtatcgcgat cgcggttttg tgccggcggc atttcgcaat
     6901 ttcctcgcgc tgcttggttg ggcgccgccg gaaggcaaag aggtcttaag caaagaggag
     6961 ttggtcgaat acttctcgct cgaaggtatc ggtcgcgcga atgcgatctt taatttccag
     7021 gag
//
LOCUS       DASSFQ010000088         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_777341, whole genome shotgun sequence.
ACCESSION   DASSFQ010000088 DASSFQ010000000
VERSION     DASSFQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777341"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFH96_02900"
     CDS             complement(<1..690)
                     /locus_tag="VFH96_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6972941.1"
                     /translation="MNWKTFWALLARDAHVARRNFVPLILQTFLQPMMFVFIFGRVMV
                     RSGYMPAAYKSLLLPGIMAISMIFTGVWAVAMPLIGEFQFTHEIEDRLLAPIENSWLA
                     IEKVLFGAAQAVVAGLAVIPAAWLLLRPVELHLFHPFAFLGITVLVALLSATGGLALG
                     CSVSQTQIGLMFSMVLTPMIFFGCTYYPWSALENFPILQKVVLVNPLVYASEGLRGTL
                     VPQFPHLPVLAV"
     gene            complement(718..1743)
                     /locus_tag="VFH96_02905"
     CDS             complement(718..1743)
                     /locus_tag="VFH96_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6972942.1"
                     /translation="MPAGIRTNELRKVFTTPPPLGAAGGFGPRRSKSKDQPAREVVAL
                     DGLSLNVQGGEIFGLLGPNGAGKSTTVGILTTRVRPTNGQAWIGDFDVWAQQVDVKRL
                     IGVVAQRPNLDFTLTAREILLFHGAYFGIDSRTRSARSTELLDRFKLTDRADQLARGF
                     SGGMMQRLSIARAMMHDPQVLFLDEPSAGLDPQTRLLLWEIIREYNRSGKTILLTTHN
                     MEEADALCQRLAIIDHGRNIALGTPAELKASVPGGYLVRVRFGSHSEDLIERLKTLTG
                     VREVRAQTFDDGDSGVDIYADRGGSLVSEIASLTAQSSVELTDVKISEPSLENLFLHH
                     TGRSLRD"
     gene            complement(1768..2661)
                     /locus_tag="VFH96_02910"
     CDS             complement(1768..2661)
                     /locus_tag="VFH96_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET6972943.1"
                     /translation="MPEGPEVRKYADALDSALAGRAIVSLAARTKDARKWLHDNEQRL
                     KGQRVERVVSHGKHLLGYIEGDFFFHSHLMMWGRWQTFGPTELPDKDRRERARIVVDG
                     GAAILLSAPIFNVGAGDPYEKIEILNTLGPDALPYRGRFNRAEFLRRLHEHESEAIGA
                     ALLNQRIVAGLGNYLRAEVLFDCKLNPWRTVGELTQRKLSCLARTVPRLTRDAYERGA
                     TAAEADRKRMASDPSLVYQPGREYGTRHMVFRRTNLPCLRCGEKIRQLRQRTMQNEDD
                     EEERTRIVYFCAKCQAADLRG"
BASE COUNT          610 a          852 c          689 g          565 t
ORIGIN      
        1 caccgccagc acaggcaaat gtggaaactg cggcacaagt gtcccgcgca gtccctcgct
       61 cgcatacacc agcggattca cgagcacaac cttttgcaga atcggaaaat tttccagcgc
      121 actccacgga taataagtgc agccaaagaa gatcatcggc gtcagcacca tgctgaacat
      181 caacccgatc tgcgtttgac ttacgctgca accgagcgcc aggccaccag tcgccgatag
      241 caacgcaacg agcactgtga tgcccagaaa cgcaaacggg tggaacaggt gcaactccac
      301 cggccgtaac aacaaccacg ctgccggtat cactgcgagt cccgccacga ctgcctgcgc
      361 ggcgccaaac aaaaccttct cgatcgcgag ccacgaattc tcaatcggcg ccagcagtcg
      421 atcttcaatc tcgtgtgtaa actgaaactc gccgatcaat ggcatcgcca cggcccacac
      481 acccgtgaaa atcatgctga tcgccatgat gcccggtaac aacagactct tgtacgcagc
      541 cggcatgtaa ccgctgcgca ccatcacgcg tccgaagata aatacaaaca tcatcggttg
      601 cagaaacgtc tgtaatataa gcgggacaaa attgcgtcgc gcgacatgag catcacgcgc
      661 caatagagcc caaaaagttt tccaattcat aatgtgtctt atgtgcttct ttgtggctta
      721 gtcgcgcaag cttcgtccgg tgtgatggag aaaaagattc tctagactcg gttccgagat
      781 ctttacatcc gtcaactcca ccgacgactg cgccgtaagg ctcgcaatct ctgaaaccaa
      841 cgagccacca cgatcggcgt aaatatctac gccgctatcg ccatcgtcga acgtttgcgc
      901 cctaacctct ctcacgcctg tcaacgtttt gagtcgttcg atcagatcct ccgagtgtga
      961 gccaaagcga acgcgcacca gataaccgcc gggaaccgac gccttcaatt ccgcgggtgt
     1021 gccgagcgcg atgttgcgtc cgtgatcgat aatcgcgaga cgctggcaca aagcgtccgc
     1081 ttcttccatg ttgtgcgtcg tcaacaggat cgtcttacca ctgcggttgt attcacgaat
     1141 gatttcccac aacagcagac gcgtttgcgg atcgagaccg gcactcggct cgtccagaaa
     1201 cagcacctgc ggatcgtgca tcatcgcgcg cgcgatcgag agacgttgca tcatgccacc
     1261 cgagaagcca cgcgccagtt gatcggcgcg atcggttagt ttgaaacgat cgagtagttc
     1321 tgtggagcga gcagaacgtg tacgcgaatc aatgccaaag tacgcaccgt gaaacagcag
     1381 tatctcgcgc gccgtgagcg tgaagtcgag attcggacgc tgtgcgacga cgccgatgag
     1441 tcgtttcacg tcgacctgtt gcgcccagac atcgaaatcg ccgatccacg cctggccatt
     1501 cgtcggtctc acgcgcgtag tgagaattcc gaccgtggtt gatttcccgg cgccgtttgg
     1561 accaagcagc ccgaagatct cgccgccctg tacgttgagc gacaagccat cgagggcaac
     1621 aacctcacgc gccggttgat ccttactctt gctgcgccgc ggaccaaagc ctcccgccgc
     1681 gcccaagggt ggcggcgttg taaagacctt acgaagttcg tttgtacgta ttccagcagg
     1741 catggcccaa ttctagccga tccgcgttta tccgcgtaaa tccgcggcct gacatttcgc
     1801 acagaagtaa acgatccttg tccgttcctc ctcgtcatcc tcattctgca tcgtcctctg
     1861 cctgagctgt cgaatcttct cgccacaccg caaacacggc agattcgtgc gccgaaacac
     1921 catgtgcctt gtcccgtact cacgtcccgg ctgatacacc agcgacggat cgctcgccat
     1981 tcgcttgcga tctgcttccg ccgccgtcgc cccacgctcg taggcatcgc gcgtcagccg
     2041 cggaactgtt ctcgctaaac aactcaactt gcgttgtgta agctcgccaa cggtgcgcca
     2101 cgggttgagc ttgcagtcga atagcacttc tgcgcgcaag tagtttccga gtcccgcaac
     2161 gatgcgttgg tttaagagcg ccgctccgat cgcttcgctt tcgtgttcgt gcaatcgtcg
     2221 cagaaactcc gcacgattaa agcgcccacg atacggcagc gcgtctggtc caagagtgtt
     2281 gaggatctca atcttctcgt aaggatcgcc tgcgccgaca ttgaatatcg gcgccgagag
     2341 taggatcgcc gcaccaccat cgaccacaat tcgcgcgcgt tcgcgtcgat ctttgtcagg
     2401 cagttcagtt ggtccaaacg tttgccatcg accccacatc atgaggtgtg agtgaaagaa
     2461 gaaatcgcct tcgatgtaac cgagcagatg ctttccgtgt gagacgacgc gctcgacacg
     2521 ctggcctttc aagcgctgct cgttgtcgtg cagccacttt cgcgcgtctt ttgtccgcgc
     2581 tgcgagcgaa acgatcgcac gtccggctaa cgccgagtcg agcgcgtctg catacttccg
     2641 cacttccgga ccttcgggca tttattcaaa aggaatcgtc ggctttgatc ccggcacgac
     2701 aacaccgccg cgatcg
//
LOCUS       DASSFQ010000089         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_34693, whole genome shotgun sequence.
ACCESSION   DASSFQ010000089 DASSFQ010000000
VERSION     DASSFQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34693"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..1370)
                     /locus_tag="VFH96_02915"
     CDS             complement(336..1370)
                     /locus_tag="VFH96_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972944.1"
                     /translation="MTFEPRKKLRIIVLVHEDLVPPDSLNGLSDKEKLEIKTEFDVTS
                     TLKKMGHEVYPIGLYNQLNVIGNSLLEHKPHIAFNLLEEFHGYPLYDQHVVSYLELMK
                     QAYTGCNPRGLTLAHDKALTKMILSYHRLLVPNFAVFPMNRKVKRPKRLKFPLLVKSI
                     SEEGSVGIAQASIVHDDEKLAQRVEFIHRQNKTAAIAEEYIKGREIYVGVIGNQNIQT
                     YAPWELVMEKLPEGAENIATLKVKWDPAYQEKVGVKTRAADLTPEQHKTLERLSKRIY
                     RYLFLSGYARLDYRMTEEGQFYLLEANPNPQIAHSEDFADSAEHSGVPYDQLLQKILT
                     VGLNYAGRSV"
     gene            complement(1367..2386)
                     /locus_tag="VFH96_02920"
     CDS             complement(1367..2386)
                     /locus_tag="VFH96_02920"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET6972945.1"
                     /translation="MAYRFPWQRLTDEQLLDLRICDLPIKIKGSFLEPHIKRLYSELN
                     SRGIRFKPHVWLAEEWFSPDGVPGIAIPFYLAHPRLMKLERRQMLEVEGGTDKECMRI
                     LRHEAGHALDTAFRLHFKRQHRELFGSFAQPYPDFYKPRPKSRRYVLHLPAWYAQAHP
                     AEDFAETFAVWLTPRLQWRRRYRGWPALEKLEYIDELMEQIAGTKPRNRTRTKVEPLS
                     ALRITLREHYRRKRERYDFEWPAVYDRDLRRIFSDDPRHKSKPSAASFLRKVRREVRQ
                     IVAEGTGVHQYTIDHVLQNMIDRCKELKLRLTTPSREARRRVMIVLTVQVMNVLHSGY
                     HRIAV"
BASE COUNT          519 a          654 c          737 g          584 t
ORIGIN      
        1 tgtttattgc ttgagtgagg agggcaccgc taaaagactc gggcatctta gcggctgaaa
       61 atggggcttt caactggcaa tgtgactgtt tcgtgacgaa acgcggaccg cgaatttagt
      121 ccgcatcgaa agaagtcacg aattcgttgc gagcgcggtc aatctgaagg attgtcgccg
      181 cccggcgact cacgcacgct gcagcttgaa atacagattc agggtaaaaa gcagagtaag
      241 aaccaacgtc ataagggcaa acactcctga cggtccaaac cgaaaataat ttgtatggtt
      301 tatttaaagt gggaatcggt ggggagtacc aggattcaca cagagcggcc cgcgtagttt
      361 agaccaaccg tgagtatctt ctggagcaac tgatcgtagg ggacgccgct gtgctcggcg
      421 gagtcagcga agtcttcgct gtgggcgatc tgcgggtttg ggttggcctc caataaatag
      481 aactggccct cttcagtcat gcggtagtcg aggcgcgcgt agccgctcag gaacaggtag
      541 cgatagattc gctttgagag tcgctcgagg gtcttgtgtt gttcgggtgt gaggtcggcg
      601 gctcgcgtct tcacgccgac tttctcctgg taagccggat cccatttcac cttgagagtc
      661 gcgatgttct ccgcgccctc gggaagtttt tccatcacca gctcccacgg cgcataagtc
      721 tggatattct gattgccgat cacgccaacg tagatctcgc ggcccttgat gtactcctcc
      781 gcaatcgccg cggtcttgtt ttgccggtga ataaactcca cgcgttgcgc gagcttttcg
      841 tcgtcgtgaa ctatggatgc ctgcgcgatc ccgactgatc cttcctcgct aatcgatttt
      901 acgagcaacg gaaacttcaa acgcttcggg cgtttcactt ttcggttcat cggaaacacc
      961 gcgaagttcg gcacgagcag acggtgatac gacaggatca tcttcgtcag tgccttgtcg
     1021 tgcgcgagcg tgagtccgcg cgggttgcag ccggtgtacg cctgtttcat cagctcgaga
     1081 tagctgacga cgtgctgatc gtagagcgga taaccgtgaa actcttcaag caggttgaac
     1141 gcgatgtgcg gcttatgctc cagcaacgag ttgccgatca cgttgagttg gttgtagaga
     1201 ccgatgggat aaacctcgtg gcccatcttc ttgagcgtcg aggtcacgtc aaactcagtt
     1261 ttgatctcca gcttttcttt gtcggaaaga ccgttcaacg aatcgggagg cacgaggtct
     1321 tcgtgaacaa gtacgatgat gcgaagtttc tttcgcggct cgaaggtcat acggcgatgc
     1381 gatgataacc tgaatggagc acgttcatta cttgcactgt cagcactatc attacacgac
     1441 gacgtgcttc tcgtgaaggt gttgttaagc gaagtttcaa ttctttgcag cgatcgatca
     1501 tgttctgcag cacgtgatcg atcgtgtact ggtgcacgcc ggtgccttcc gcaacgatct
     1561 gtcgcacttc acggcgcacc ttgcgcagaa aactcgccgc gctcggtttt gacttgtgcc
     1621 ggggatcgtc ggagaagatc cggcgcagat cgcgatcgta aacagcgggc cattcaaagt
     1681 cgtaacgttc gcgtttgcga cggtagtgtt cgcgcagagt gattcgcaac gctgaaagag
     1741 gttcgacttt cgtgcgtgtg cggttccgtg gtttcgtgcc cgcgatctgt tccatcaact
     1801 cgtcgatgta ctcgagcttt tccaacgcag gccagccgcg ataacggcgt cgccactgca
     1861 aacgcggcgt gagccacacg gcgaatgtct cggcgaagtc ttccgccggg tgcgcctgcg
     1921 cgtaccacgc gggcaggtgc aaaacgtagc ggcggctctt cggccgtggt ttgtagaagt
     1981 cgggatacgg ctgtgcgaac gagccgaaca gctcgcggtg ttggcgtttg aagtggagcc
     2041 gaaacgccgt gtcgagcgca tggccggctt cgtggcgcag gatgcgcatg cactccttgt
     2101 cggtcccgcc ctcgacttcc agcatctggc ggcgttcgag cttcatcagc cggggatggg
     2161 cgaggtagaa cgggatcgcg atcccgggca cgccgtcggg cgaaaaccac tcttccgcca
     2221 gccaaacatg gggtttgaag cggatgccgc gggagttgag ctcgctatag agccgcttga
     2281 tatggggctc gagaaacgag cccttgattt tgataggcaa atcgcagatg cgcagatcta
     2341 gcaactgctc atccgtgagt cgttgccagg ggaatctgta tgccacggcg gcaaattgta
     2401 aacgcctcgc gtaatccgtt caaacgtggt acttttcctg tccgccttgc gcgtttgaga
     2461 tcgagatcaa accgggagtc ccaatgctaa aaac
//
LOCUS       DASSFQ010000090        13147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1676656, whole genome shotgun sequence.
ACCESSION   DASSFQ010000090 DASSFQ010000000
VERSION     DASSFQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676656"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="VFH96_02925"
     CDS             complement(<1..1835)
                     /locus_tag="VFH96_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET6972946.1"
                     /translation="MAPSALAQDITTLEKIGDVVSLTKTDKSVTLICKDNSQVQLTIL
                     APDLVRVRASFTKPIPARDHSWAIAKNDWSTPRWTVTESPTTITITTDELEVTIQRSP
                     LLISFRDARDHQLLNTDERPMSYDAKASLTGMMFDPKAGPFVAASKKLGFDEHFYGLG
                     EKASRLDKRRSSFANWNSDTPGYIEGRDPIYQTIPFYIGLQRGIAYGIFFDNSYRSYF
                     DFGKSSQQRAWFGAEGGELNYYFFHGPSIKKILGRYADLTGHMPLPPLWALGNQQSRW
                     SYYPDTMVEEVVNEYRKRDLPLDVVHLDIDYMHGYRVFTFNKDRFPDPKALTDKLASQ
                     GVKVVTIVDPGIKHPVDKNERYHAFDEGLEKNYFQRRRNGDLFVPRVWPGESVFVDYT
                     MPEARRWWGDLHRVYTDNGIAGIWNDMNEPSDFVDQTGKNQLDVVSYDEGEKSTHAKN
                     RNTFALLMSRATYEGLERLRPDRRPYVITRAAYAGIQRYATMWTGDTNSTWEALALNV
                     PMFTSLGLSGEPFVGSDVGGFIGRGDGELLVRSYQVSFLAPFCRNHKVIDGYDQEPWR
                     FGKYYEDIIRKYLKLRYALMPYLYTALEEAHRTGVPPFRPLLLNY"
     gene            complement(1939..2886)
                     /locus_tag="VFH96_02930"
     CDS             complement(1939..2886)
                     /locus_tag="VFH96_02930"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET6972947.1"
                     /translation="MVAADKTDSGLIFAADLGGTHLRGATVDQKGRIQSRFKQNTPRG
                     KEAIVDAIVAAVHDFQKQAGISAVSLSVPGTVKVEEGLIVKAPNLPCLDGFRLAAALS
                     SRLDVPAVLENDANAAAVGEMWQGAAVGCKTIICLTLGTGVGGGIILDGKLWRGVDGA
                     AAEIGHMCVDPFGGVACPCGSRGCLEVFASATAIVRMTREASPRYPDSILQAKDDLTA
                     AAIFEAGKQGDELALEIFRRMGVYLGIGLANLINILNPEMIVIGGGVVNGWDLFEKHM
                     HQQVEERAFPLLAERVKIVRAKCGDDAGLLGAARLALRD"
     gene            complement(2870..4735)
                     /locus_tag="VFH96_02935"
     CDS             complement(2870..4735)
                     /locus_tag="VFH96_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET6972948.1"
                     /translation="MIRIVLLILFGCQVVMAQGGDAYWATAAKNGFGTSNTLASKVWF
                     TLAEGVMTEVFYPRADMPSVRSLQFLIITGGQTENEAEDTVHRLELPNPASLTFRQIN
                     RAKSGAYAITKTYIADPRRSSVLIRVDFTTSLNAQLYINDVPLNDKSVLVSNCGLPKL
                     QRKQCTLAVGFGETIAKATATARASLARGFAAVMREYELGWQQYVARLPRVEKHQKQF
                     NMAAMVLKGLEDKTFRGAVIASPSTPWGGGQKPTQPPVSGYHAVWARDLYHMATAFDA
                     IGDRATANRLLDYVFRVQQRGDGSFPQNSWVDGRPIGNGLQMDQVALPLVLAYQFDRK
                     DRQSWLSHIKPGADFIVRNGPRTDQDRWEERSGYSPSSLAAQIAGLVCAAEIAKANRD
                     DASAKRYLDTAGNWAQNVEKWTVTKPDGGSGYYLRITANNDPNDGTKMEINSSSLVVD
                     ERKVLDAGFLELVRLGVKAPRDPLIVESLKLIDQLIKVKTPVGDAWYRYNHDAYGETP
                     DGGKYDGHNGVGRLWTLLTGERGEYELAAGDIASARKRLDTLAGFANDGLMIPEQVWD
                     REESPTPSLRFGAGTGSATPLAWSMAQFIRLAMSIERGRNVETPRVVWERYGSRG"
     gene            complement(4742..6028)
                     /locus_tag="VFH96_02940"
     CDS             complement(4742..6028)
                     /locus_tag="VFH96_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6972949.1"
                     /translation="MLKPRPRITRIVCGLIACLVAVSGTPAQRPVRDWVRDGVIYEIY
                     PRAFSQQGNFNAITARLDELKDLGVTILWLMPIHPIGQEKKKGTIGSPYAVRDYYGIN
                     PDYGTAEDLKRLVREAHARGLKVIIDIVANHTSWDSVLMKHPEFYKRDAKGNITYPHD
                     WYDIAALNYGNQELRRYMTDMLKYWIREFDLDGFRCDVAGEVPTDFWENARRELEQIK
                     PDIVMLAEAHKAELQVKAFDLDYSWPLHSALTNVVQGRGRASDLREEWEKEVREWPKG
                     ALHMRFSDNHDERRAIARFGEPAALAASAFVFTLDGVPMIYNGMEVGDTTESGAPALF
                     EKMPIFWTFAERRKEFPKFYKDMMARRRSSMALRRGTLEWVPNSDEARVVTFVRRAGN
                     EEVLVAINFSSRPFVGSVAGRKVSLDAWGYRIFEKK"
     gene            complement(6015..7316)
                     /locus_tag="VFH96_02945"
     CDS             complement(6015..7316)
                     /locus_tag="VFH96_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6972950.1"
                     /translation="MNKPRLSFWQIWNMSFGFLGIQFGWGLQLANMSPIYKYLHAEES
                     SLPYLWLAGPITGLIIQPIIGSMSDRTWNKLGRRRPYFLAGAILASVALFFMPDSSAV
                     WMAAGLLWILDASINITMEPFRAFVADKLPEEQRTLGFVMQSFFIGIGQTLANGLPYL
                     FTALGVVGVMASGIPLSVDYAFKIGAIVFLVAVLWTVLTTKEYPPEDMEAFERMRREK
                     KGCFGGFAEIFSSFAEMPSTMKQLAVVQFFTWFALPCMWQFFGIAVARHVFGAPSEAS
                     PLFAQGTEWGGLCFAVYNVVCFLVAFMLPSLANATSRKTVHIIALLCGGIGLISVLFI
                     TNKFMLFASMAGVGIAWASILSMPYVMLSTAVPPARMGVYMGVFNLFIVIPQIVMSLI
                     VPRIYNNVLGGDPRNAVVLGGISLLIGAVTVWGVREVHAET"
     gene            complement(7346..9208)
                     /locus_tag="VFH96_02950"
     CDS             complement(7346..9208)
                     /locus_tag="VFH96_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6972951.1"
                     /translation="MTRIFKNRSALIRLFCVHLWLCFGVAAQAPEILKVDPPSWWTGS
                     TVNPVRLMIHGRNLHGARVEVQGLRIVNAPKISGTYIFADVFIPPNTRAGKRSISVTT
                     PRGSVNSTFDVLPSLNRAGRFQGFSPNDVIYLIMIDRFADGDPSNNGGIYDRKNKFYY
                     HGGDLQGVIDHLPYLKELGVTAIWLTPWYDNYDRLNQIELKEDKPSTGFHGYNPQDFY
                     AVEEHFGTLAKLRELVEAAHRSGIKIIQDQVMNHTGPYHPWVDDPPTPTWFNGTKSKH
                     LKNVFQTWALHDPRAVESLKRETMEGWFLDFLPDLNQHDEEVSRYLIQNTLWWIGTTG
                     LDGIRMDTWQYVPNSFWHDWTRALKREFPNFKVVGEVKDGDVVHTSFFQGGRVRFDGV
                     DSGLDSLLDFPLFYPIRHAFAEGKPIDEIPKTLAKDYLYTNPEILVTLLGGHDDGRFM
                     SEKGATIAGLKLANAFVLTTRGVPQLYYGDELAMEGGDEPTTRGDFPGGFGGDARNAF
                     SAGGRTKDEQELFEYIKRLTTLRREYEVLRTGELLNLYVSEQQYAYARRGTRGAVIVV
                     INNENDAASIEFDVSRVASGGVFKDRLGVSQPAQINNGKMQVTLPKRSAAIFVR"
     gene            complement(9215..10738)
                     /gene="malQ"
                     /locus_tag="VFH96_02955"
     CDS             complement(9215..10738)
                     /gene="malQ"
                     /locus_tag="VFH96_02955"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET6972952.1"
                     /translation="MINKKRLSGVLLHPTSFPGPYGIGDLGPEAYKFVDFLVAAGQSL
                     WQVLPLGPTGYGDSPYACYSAFAGNTLLVSPERLVNEGLLSHRRDAEDAEVTQRINFG
                     EVHQYKQQVLREAYEGYTRTTDTSLRSAFETFAQQHSNWLEDYALFRALKDAHGGVAW
                     NEWEPALVRRTPAALEHARDELREEVEAHMFYQFLFFRQWFALKAYANDRGISVVGDL
                     PIFVAQDSADVWTNPEQFKLDKNGKPIVVAGVPPDYFSSTGQLWGNPLYNWERMLADG
                     FKWWIERVRATLTVVDIARVDHFRGFAACWEIPGGDKTAERGQWVEAPGKELFTAIRK
                     TLGELPIIAEDLGVITPDVVALREEFGFPGMRILQFGFGSDSKNIDLPHNYVPNVVAY
                     TGTHDNDTTVGWFQSVAGEGSTRTAEQIERERKFCMDYLNTDGKEIQWDFIRGVLGSV
                     ANTAIVPLQDLLGLGTEARMNLPNSTEGNWAWRYKAGALTDEIGTRLKQLSELYGRN"
     gene            complement(10750..12549)
                     /locus_tag="VFH96_02960"
     CDS             complement(10750..12549)
                     /locus_tag="VFH96_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6972953.1"
                     /translation="MAGSCSPVYAQTPMVTKVDPPSWWAKHTISPVRLLVRGANLRGG
                     HVRPLNPEMRASAISINERGTYLFVDVEISPSLRPGAYPLLVESASGSTMIPFRLETP
                     LDAQSNFQGITTDDVIYLIMTDRFADGDPSNNAPKDSPHEANDRNNPRSFHGGDLRGI
                     INHLPYLKDLGVTALWLTPWYDNWNGVNRCDKPWCPNTYYHGYHAIDYYAVEDRFGDL
                     KTLRELVQKAHALGIKIIQDQVANHVGSQHPWVKDPPLANWFHGTLSQHQLNNFHNAA
                     LLSPHSNLRDVRNTLDGWFSDDLPDMNQDEPEVARYEIQNALWWVGITGIDGIRQDTI
                     QYMPRFFIRDLSNALHRQYPKLWMVGEVFERDAAHTAFFIGGHTGWDGVDTKLDSVFD
                     FALWNASLSVFTNKAPLRLLPHQLKYDALYPDPSKVTVLANNHDTMRFMSLEGATMAG
                     AMMHVAFVLSVRGIPQLYAGEEIAMEGKEDPDNRRDFPSDAFRAEGRKPKEREMFEWT
                     RSWIKLRRDHAAMRAGKLIDLFVDDETYVFARQLGEEKIVVAFNRESKPKQVVIPVEM
                     RLRPLIGDTSARVEKGETTLMLPAQTAVALRAF"
     gene            complement(12645..>13147)
                     /locus_tag="VFH96_02965"
     CDS             complement(12645..>13147)
                     /locus_tag="VFH96_02965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6972954.1"
                     /translation="LLGYAARAEGQYDVAVEAFQKALKSQPDNPDVLGNLGYIASQRG
                     ENVEAERLLRRAIELDPKSFPPYHDLGRLLVKLKRYEEAVPLLTRGIELNAKDPGVHY
                     QLFLAYSRLKRKDEADKELAEFKRLDEVNQHGATPLTPMKDGETLPPLPVVAAGDSAK
                     PRTPNN"
BASE COUNT         2999 a         3990 c         3323 g         2835 t
ORIGIN      
        1 tggtagttga gcagcagggg ccgaaacggc ggcacgcctg tgcgatgcgc ttcttccaaa
       61 gccgtataga gataaggcat gagggcgtag cggagtttta gatacttgcg aatgatgtct
      121 tcgtaatact ttccaaagcg ccagggctcc tgatcgtacc cgtcgatcac tttatgatta
      181 cgacaaaacg gcgcgaggaa actcacttga tacgatctca ccagcagctc gccatcccca
      241 cgaccgataa atccaccaac atcgctgcca acgaacggct cgcctgacaa accaagcgat
      301 gtaaacatcg ggacgttgag cgcgagcgct tcccacgtac tgttcgtatc gcccgtccac
      361 atcgtcgcgt aacgctgaat gcccgcatac gccgcgcgcg tgatcacgta cggccggcgg
      421 tcgggtcgca gtcgctctaa tccttcgtac gtcgcgcgcg acatcaacaa agcaaacgtg
      481 tttcgattct ttgcgtgggt tgatttctcg ccttcatcgt agctgaccac gtctaactgg
      541 ttcttgccgg tctggtcgac gaagtcagac ggctcattca tatcattcca gatgccggcg
      601 ataccgttgt cggtgtaaac gcgatgcagg tcgccccacc aacgacgcgc ctcaggcatc
      661 gtgtaatcaa caaaaacact ttcgccgggc cagacgcgcg gcacaaacag atcgccattg
      721 cgacgtcgct gaaagtagtt cttctccaat ccttcatcaa atgcgtgata gcgctcgttc
      781 ttgtccaccg gatgcttgat gccgggatcg acgatcgtca cgaccttcac gccctgactg
      841 gcgagtttgt ccgtcagtgc tttcggatcg ggaaaccgat ctttgttgaa agtaaaaacg
      901 cgatacccgt gcatgtagtc tatgtcgaga tgcacgacgt cgagcggcaa gtcgcgtttg
      961 cgatactcgt tcaccacttc ttcaaccatc gtgtcgggat aatagctcca gcgactctgc
     1021 tgattgccga gcgcccagag cggcggcagc ggcatgtggc ccgtgagatc ggcgtagcgg
     1081 ccgagaatct ttttgattga tggaccatgg aagaagtagt agttcagctc gccgccttca
     1141 gcgccaaacc acgcgcgctg ctgcgacgac ttcccgaaat caaaatagct gcgataactg
     1201 ttgtcgaaaa agatcccgta agcgatgccg cgctggagac cgatgtaaaa cgggatggtc
     1261 tgatagatcg gatctctgcc ttctatataa ccgggcgtgt ctgagttcca gttggcgaac
     1321 gagctgcgac gcttgtcgag acgcgaagcc ttttcgccga gaccgtagaa atgttcgtcg
     1381 aagccgagct tcttcgaggc ggcgacgaat ggtccggctt tgggatcgaa catcatgccg
     1441 gtgaggctag cctttgcgtc gtaggacatt gggcgctcgt cggtgttgag gagttgatgg
     1501 tcacgcgcgt cgcggaagga gattaataaa ggtgaacgct ggatcgtgac ttcgagctcg
     1561 tcggtagtga tggtgatggt cgttggtgat tcggtgactg tccagcgtgg tgtggaccag
     1621 tcgtttttgg cgatcgccca cgaatgatcc cttgcgggga tcggcttggt gaatgaggcg
     1681 cggacacgaa cgagatccgg agcgaggatt gttagttgga cctgcgagtt atccttacag
     1741 atgagggtaa cggatttatc ggttttggtg agactcacca cgtcgccaat cttctcgagt
     1801 gtggtgatgt cctgcgcaag ggcactggga gccaacagca atataagtat gagaggacga
     1861 agcatagttt tttacaagat tacaggattt acaagatgaa tcctgtcaat tctgtaaatc
     1921 ctgtcatcct gtataaaatt aatctcgaag cgcgaggcgc gccgccccca acaaacccgc
     1981 atcatcacca cacttcgcac gcacgatctt cacgcgctca gccagtaacg gaaacgcgcg
     2041 ctcttcaacc tgctggtgca tgtgtttctc aaacagatcc caaccattca ccacaccacc
     2101 accaatcacg atcatctctg gattgaggat attgatcaga ttcgccagac caatcccaag
     2161 ataaacaccc atgcgtctga aaatctccag cgccagctcg tcaccctgct ttccggcttc
     2221 gaagatcgcc gccgcggtga gatcatcttt cgcctgcaag atcgaatccg gatagcgtgg
     2281 actcgcctca cgcgtcattc gcacgatcgc cgtcgccgac gcaaaaacct ccaggcaacc
     2341 acgactgcca cacggacacg caaccccacc aaaaggatcg acgcacatgt gtccaatctc
     2401 agccgctgca ccatccacac cacgccacaa cttgccgtcg agaatgatcc cgccgcccac
     2461 acccgtcccg agcgtcagac agatgatcgt cttgcaacca acggccgcac cctgccacat
     2521 ctcaccgact gccgcagcgt tcgcatcgtt ctccaacacc gccggaacat cgagacgact
     2581 cgacaaagcc gcagccagcc gaaacccatc tagacatgga aggttcggcg ccttcacgat
     2641 caaaccctcc tcaaccttca ctgtcccggg cacagacagc gatacagcgg agatgccggc
     2701 ctgtttctga aaatcatgaa ccgcagcgac aatcgcatcg acaatcgcct ctttgcctcg
     2761 tggcgtgttc tgcttaaacc gcgactgaat tcgccccttc tgatccaccg tggccccgcg
     2821 caagtgcgta ccgccaagat cggcagcgaa aatcagtcct gaatccgttt tatccgcggc
     2881 taccataccg ttcccacacg actctcggtg tctccacatt ccgcccgcgc tcgatactca
     2941 tcgccagtcg aataaactgc gccatggacc aggctaacgg cgtcgccgat ccggttcccg
     3001 caccaaaacg aagactcgga gtgggactct cctctcgatc ccaaacctgc tccggaatca
     3061 tcaatccatc atttgcgaaa cccgcgagcg tatcgagtcg cttgcgcgca gacgcgatat
     3121 cacccgcggc gagctcatac tcaccgcgtt cgcccgtcaa caaggtccaa agccttccga
     3181 caccgttgtg cccgtcgtac tttccgccgt cgggagtttc gccatacgcg tcgtgattgt
     3241 agcggtacca cgcgtcacca accggcgtct taacttttat taattgatcg ataagcttca
     3301 acgactcgac gatcaacgga tcgcgcggcg ctttgacacc aagccggacc agttcgagaa
     3361 aacccgcgtc aaggaccttt cgctcatcga caacgagact gctgctgttg atctccatct
     3421 tcgtgccgtc gttcggatca ttgttagcag tgattcgcag gtaataacca ctgccgccat
     3481 caggtttggt aacagtccac ttctcgacgt tctgcgccca gttgcctgcg gtatcaagat
     3541 accttttggc tgacgcatcg tcgcgattgg cctttgctat ctctgccgca cacacaagcc
     3601 cggcgatctg cgctgccaga ctcgaaggcg aatagccaga cctctcttcc caacgatcct
     3661 gatccgtccg cggcccatta cgcacgatga aatcggctcc gggtttgata tgactgagcc
     3721 acgactgccg atctttacga tcgaactgat aagccagcac gagcggaagg gccacctggt
     3781 ccatctgtaa tccattgccg atcgggcgtc catcgaccca cgagttttgc ggaaagctgc
     3841 catcgcctct ttgctgcacg cggaacacgt agtcgagcaa acgattcgcg gtagcgcgat
     3901 ctccgatggc gtcaaaagct gtcgccatgt gataaagatc gcgagcccag acagcatggt
     3961 agccactgac gggcggttgc gtcggttttt gaccaccacc ccagggcgtc gaaggcgaag
     4021 cgatcacagc accccgaaaa gtcttgtctt cgagtccttt taggaccatc gccgccatgt
     4081 tgaattgctt ctgatgcttc tcgacacgtg gcaacctcgc gacgtattgc tgccagccga
     4141 gttcatattc acgcatgact gcggcgaaac cacgcgcgag cgatgcacgc gctgtcgccg
     4201 tcgcttttgc tatcgtctca ccgaacccaa ccgctaacgt acactgtttc cgctgtagct
     4261 ttgggagacc gcaattcgac accagtacag acttatcatt caaaggaacg tcattgatat
     4321 acaactgcgc attgaggctg gtcgtgaaat cgacccggat tagcactgag ctacgccgcg
     4381 gatccgcgat gtaagttttt gtgatcgcgt aagcaccact ctttgcacgg ttgatttgcc
     4441 ggaaggttag cgacgccgga ttcggtagtt cgagcctgtg cactgtgtct tcggcttcgt
     4501 tttcagtctg cccgccagta atgatcagaa actgaagact gcgcacgctc ggcatatcgg
     4561 ccctcggata aaagacctcc gtcatcacac cctcagcaag cgtgaaccaa accttcgacg
     4621 cgagcgtgtt cgacgtccca aacccattct tcgccgcggt ggcccaatac gcatcaccgc
     4681 cctgcgccat caccacctgg cagccaaata gaatcaataa aacaatccgt atcatccgtg
     4741 gctacttttt ctcaaagatt cgataacccc aggcatccag cgagaccttc cgtccagcga
     4801 cagatccaac gaacgggcgg ctcgaaaagt taatcgccac caacacttcc tcattccctg
     4861 cccgacgaac aaaagtcacc acgcgcgcct catccgagtt tggtacccat tcaagcgttc
     4921 cccgccgcag cgccatactc gatcgccgcc gcgccatcat gtctttgtaa aacttcggaa
     4981 actccttgcg acgctcggca aacgtccaga agatcggcat cttctcaaac aacgcgggcg
     5041 cgcccgactc agtcgtatca ccaacctcca tcccgttgta gatcatcggc acgccgtcga
     5101 gcgtgaacac aaacgcagac gccgccagcg ccgccggctc gccaaaccgc gcaatcgcac
     5161 gtcgctcatc atggttatca gaaaacctca tgtgcaacgc gcctttgggc cactctctga
     5221 cttccttctc ccactcttca cgcagatcgg acgcacggcc acgcccctgc acgacgttcg
     5281 tcaacgcgct gtgcagcggc cacgagtaat cgagatcgaa tgccttcact tgcagctcgg
     5341 ctttgtgcgc ttccgccagc atcacgatgt caggcttgat ctgctccagc tcgcgacgcg
     5401 cgttctccca gaaatccgtc ggcacttcac ccgctacatc acaacgaaac ccatcgagat
     5461 caaactcgcg gatccagtac ttgagcatgt cggtcatgta gcgtcgcaac tcctggttgc
     5521 cgtaattcag cgcagcgatg tcataccagt cgtgcgggta agtgatgttg ccctttgcgt
     5581 cgcgcttgta aaactccggg tgcttcatca atacgctatc ccacgacgtg tgattggcga
     5641 cgatatcgat gatgaccttc aacccgcgcg cgtgtgcctc acgcacgagt cgcttgagat
     5701 cctcagcagt gccatagtcc ggattaatcc cgtaataatc acgcaccgcg tacggacttc
     5761 caatcgtccc cttcttcttc tcctgcccga tcggatggat cggcatcaac caaagtatcg
     5821 tgaccccgag atccttaagt tcatcaagcc gcgccgtgat cgcattaaaa ttcccctgct
     5881 gcgaaaacgc gcgcggataa atctcataga tgactccatc acgcacccaa tcacgcacgg
     5941 gccgctgcgc aggagtaccc gagaccgcca ccaaacatgc gatcaatccg caaacaatcc
     6001 gtgtaatccg cggcctaggt ttcagcatga acctcacgca ctccccacac tgtcaccgcc
     6061 ccgatcaaca gactgatccc accaagcacg accgcattcc tcggatcccc acccaacacg
     6121 ttgttataaa tccgcggcac gatgagcgac atcacgatct gcggaatcac gatgaagaga
     6181 ttgaacacgc ccatgtaaac acccatacgc gccggcggca ccgccgtcga taacatcacg
     6241 tacggcatcg acagtatcga cgcccaagcg attccaacgc ccgccatgct cgcgaacaac
     6301 ataaatttat tcgttataaa caaaactgag atcagaccga ttccaccaca caacaacgcg
     6361 atgatgtgca cagtcttacg actcgtggcg ttcgcgagtg acggcaacat gaacgcgact
     6421 aaaaaacaaa ccacgttgta aacagcgaaa cacagcccgc cccactccgt tccctgcgca
     6481 aacagcggcg acgcctcact cggcgcacca aacacgtgtc gcgcgactgc gatcccaaag
     6541 aactgccaca tacacggcaa cgcgaaccac gtgaaaaact ggaccaccgc tagctgtttc
     6601 atcgtcgaag gcatttctgc aaatgaagaa aagatttccg cgaagcctcc aaaacatccc
     6661 ttcttctcac gtcgcatgcg ctcgaacgct tccatgtctt ccggtggata ttcttttgtc
     6721 gttaaaacag tccagagcac tgcgacgaga aacacgatgg cgccgatctt gaacgcgtaa
     6781 tcgactgaaa gcggaattcc actcgccatc acacccacga caccaagcgc ggtaaacaag
     6841 taaggcaatc cattcgcgag tgtctgaccg atgccgatga agaaactctg catcacgaag
     6901 ccgagcgtgc gttgttcttc cggcagcttg tcggcgacaa acgcgcgaaa cggttccatc
     6961 gtgatgttga tgctcgcgtc gagtatccag agcaggcccg ccgccatcca cacggcggac
     7021 gaatcaggca tgaagaagag cgccacactg gcgagaatcg caccggcgag aaaataagga
     7081 cggcgacgac caagcttgtt ccaggtgcgg tcgctcatcg agccgatgat gggttgaatg
     7141 atcagaccag tgattggacc agcgagccag agatacggaa gcgagctttc ctcggcgtgc
     7201 aggtatttgt aaatcgggct catgtttgcg agctgcaaac cccagccaaa ctgaatgccg
     7261 agaaatccga aactcatgtt ccagatctgc cagaagctca agcgtggttt gttcattggc
     7321 gattccttaa cacggattta gcggattagc ggacaaagat tgccgcggat cgtttcggta
     7381 aagtcacctg catcttgccg ttattgatct gcgcgggttg gctcacaccg agtcgatctt
     7441 tgaaaactcc acccgatgcg acgcgtgaga cgtcaaactc tatcgaggcc gcatcatttt
     7501 cattgttgat tacgacgatc accgcacccc tcgtcccgcg acgcgcgtag gcgtattgct
     7561 gctcagacac gtagagattc aacagctccc cggttcgcag gacttcatat tcgcggcgaa
     7621 gagttgtgag acgcttgatg tattcaaaca actcctgctc gtctttagtg cgacccccgg
     7681 cggagaaggc atttcttgca tcgcctccaa acccgccggg gaagtcgcct cgcgtggttg
     7741 gttcgtcacc accttccatc gcaagctcat ctccgtagta aagctgtggc acaccgcgcg
     7801 tcgtcaacac aaacgcattc gccagtttca atcctgcaat cgtcgcgcct ttttcactca
     7861 tgaagcgacc atcgtcgtga ccaccgagca aggttacgag aatctcggga ttcgtgtaga
     7921 gatagtcttt cgcgagcgtc ttcggaatct catcgatcgg cttgccctcg gcaaacgcgt
     7981 gtcgaatcgg gtagaagagc gggaagtcga gcagcgaatc gagtccggaa tcgactccgt
     8041 caaaacgaac tctgccgccc tgaaagaacg acgtatggac cacgtcgccg tctttcactt
     8101 cgccgacgac cttgaaattc ggaaactcac gcttcaaagc cctggtccaa tcgtgccaaa
     8161 aggaattcgg cacgtattgc cacgtgtcca tgcgaatacc gtcgagtccg gtcgtgccga
     8221 tccaccaaag tgtgttttga atcaaatatc gtgagacttc ttcgtcgtgc tggtttagat
     8281 ccggcaggaa atcgagaaac cagccctcca tcgtttcgcg tttcaacgac tcaaccgcgc
     8341 gtgggtcgtg tagcgcccac gtttgaaaga cgttcttcag atgcttcgac ttggtgccgt
     8401 tgaaccacgt gggcgtcggt ggatcgtcga cccacggatg ataagggccg gtgtgattca
     8461 tcacctggtc ctggatgatt tttatgccgg agcgatgcgc agcctcgacc agctcacgca
     8521 gtttcgcgag cgtgccgaag tgttcctcga ccgcgtaaaa atcctgcggg ttgtagccgt
     8581 gaaatccggt actcggttta tcttccttca gctcgatctg gttgaggcga tcatagttgt
     8641 cataccacgg cgtcagccag atcgcggtaa cgccgagctc ctttaaatat ggcaagtgat
     8701 cgattacgcc ctgaagatcg ccgccatggt aataaaactt gttcttgcga tcgtagatgc
     8761 caccgttgtt tgaaggatcg ccgtcagcga aacgatcgat catgatcagg tagatcacgt
     8821 cgttgggcga gaacccctga aagcgaccgg cgcgattcag gcttgggagc acgtcgaagg
     8881 tagagttaac cgaaccgcgc ggcgtcgtga ctgatatcga gcgcttacca gctcgtgtat
     8941 tcggtggaat aaagacgtcc gcgaagatgt acgtgccgct gatcttcggt gcgttgacga
     9001 tacgaagtcc ctgaacctct acgcgcgcac catgtaaatt acgcccatga atcatgagcc
     9061 gcactggatt aaccgtactg ccggtccacc aactcggcgg atcgacttta aggatctccg
     9121 gcgcctgcgc agcaacacca aaacataacc acagatgaac acagaaaaga cggatcagcg
     9181 ctgatctgtt tttaaaaatc cgtgtcatcc gtggctagtt cctaccgtag agttctgata
     9241 actgcttgag tcgagttccg atctcatctg ttaacgcgcc ggccttgtat ctccacgccc
     9301 agtttccctc ggtactgttc gggaggttca tgcgtgcttc cgtccccagg cccaaaaggt
     9361 cttgtaaggg aacgatcgca gtgttggcga cggaccctaa aactccgcga atgaagtccc
     9421 attgaatctc tttgccgtca gtattcagat agtccatgca gaacttccgc tcgcgctcga
     9481 tctgttccgc agtacgcgtc gagccctcgc ctgcgacact ctgaaaccag ccgacagtcg
     9541 tgtcgttatc gtgcgtgccc gtgtaagcga cgacgttggg aacgtagttg tgcggcagat
     9601 cgatgttctt cgaatcacta ccgaagccga actgaagaat gcgcattccc ggaaagccaa
     9661 actcctcacg caacgcgacc acgtcgggcg tgatcacgcc gagatcctcg gcgatgatcg
     9721 gcagctcacc aagcgtcttg cgaatcgcgg tgaacagttc ctttcccggc gcttcgaccc
     9781 actggccgcg ctcggcagtc ttgtcgccgc cgggaatttc ccaacacgcc gcaaagccgc
     9841 gaaagtggtc cacgcgcgcg atgtcgacga cagtcagcgt ggcgcgcacg cgctcgatcc
     9901 accatttgaa accgtcggcg agcatgcgtt cccagttgta gagtggattg ccccagagct
     9961 gtccggtgct gctgaagtaa tccggcggca caccggcgac gacgatcggt tttccgtttt
    10021 tgtcgagctt gaactgctcg gggttagtcc aaacgtcagc cgagtcctgc gcgacgaaga
    10081 tcggcagatc gccgacaacg ctgatgccac gatcgttcgc gtatgccttc aaagcaaacc
    10141 actgccggaa gaacagaaac tggtagaaca tgtgcgcttc gacttcttcg cgcagctcgt
    10201 cacgcgcgtg ctcgagtgcc gcgggcgtgc gacggacaag cgcgggttcc cactcgttcc
    10261 aggcaacacc gccgtgcgcg tctttgaggg cacgaaagag cgcgtaatct tcgagccagt
    10321 tggaatgttg ttgagcgaac gtttcgaacg cgctgcgcaa agacgtgtca gtagtcctgg
    10381 tatacccctc atacgcttcg cgtaatacct gttgtttata ttgatgcact tccccgaaat
    10441 tgattctctg cgtaacctcg gcgtcctctg cgtctctgcg gtgagaaagc aatccttcgt
    10501 tgaccaaccg ttccggactg acgagcagtg tattgccggc gaaagccgaa tagcacgcgt
    10561 aaggcgagtc gccatagccc gttggtccaa gcggcaaaac ctgccagaga ctctgacccg
    10621 ctgctaccaa aaaatcgacg aacttgtacg cctccggtcc cagatcgcca atcccgtacg
    10681 gccccggaaa cgacgtcggg tgcaacaaga ctccgctcaa cctcttcttg ttaatcatga
    10741 aaatcctgtc taaaacgctc tcaatgccac cgcggtctgt gccggcaaca tgagcgttgt
    10801 ctcacccttc tcaactcgtg cgcttgtgtc accgatcagc ggtcgcaacc tcatctcaac
    10861 cggaatcacc acctgcttcg gtttactctc acgattaaac gcaaccacaa tcttctcttc
    10921 tccaagctgc cgcgcgaaca cgtacgtctc atcatcaaca aacagatcga tcaacttccc
    10981 cgcgcgcatc gcggcatgat cacgccgcaa tttgatccac gaacgcgtcc actcaaacat
    11041 ctcgcgctct ttcggcttgc gcccctcagc ccgaaacgca tcactcggaa aatcacgacg
    11101 gttatccgga tcttccttcc cttccatcgc gatctcttca cccgcataga gctgcggaat
    11161 cccgcgcaca ctcaagacaa aagcgacatg catcatcgcc cctgccatcg tcgcgccttc
    11221 cagcgacatg aagcgcatcg tgtcgtgatt gttcgcgagc actgtgactt tcgacggatc
    11281 gggatacagc gcgtcgtact tcaactgatg cggcaacaaa cgcagcggcg ccttgttcgt
    11341 gaacacagac agcgacgcat tccacaacgc aaaatcgaaa accgaatcga gcttcgtatc
    11401 cacaccatcc cagcccgtgt gaccgccgat gaaaaacgcc gtatgcgcag cgtcgcgctc
    11461 aaacacctcg ccaaccatcc acagctttgg atactgccgg tgcagcgcgt tggacagatc
    11521 gcgtatgaag aaacgcggca tgtactggat cgtgtcctgg cgaatgccgt caatgccggt
    11581 gatgcccacc caccacaacg cattctggat ctcgtagcgc gcgacttcgg gctcgtcctg
    11641 gttcatgtcc ggaagatcgt cgctgaacca gccgtcgagc gtgttgcgta catcgcgtag
    11701 gtttgagtgc ggcgaaagca gcgcggcgtt atgaaagttg ttgagctggt gctgcgagag
    11761 tgtgccgtgg aaccagttcg cgagtggtgg atctttaacc cacggatgct gcgagccgac
    11821 gtggttcgct acctggtcct gaatgatctt gatgccgagc gcgtgcgctt tttggaccag
    11881 ctcgcgcaac gtcttgaggt caccgaaacg atcttcgaca gcgtagtaat cgatcgcgtg
    11941 ataaccgtga tagtacgtgt tcgggcacca cggtttatcg caacggttca cgccattcca
    12001 gttgtcatac cagggcgtta accagagcgc ggtgacgccg agatctttca gatatggaag
    12061 gtggttgata atgccgcgca gatcgccgcc gtgaaaactg cgcgggttgt tgcgatcgtt
    12121 tgcttcatgt ggagaatctt tcggagcgtt gtttgatgga tcgccgtcgg caaaccgatc
    12181 ggtcataatc aggtagatca cgtcgtcggt ggtgatgcct tgaaagttgc tttgcgcgtc
    12241 gagcggtgtt tcaaggcgga acggaatcat cgtgctacca ctcgcactct cgacaagcag
    12301 tggatacgcg cccggacgca gcgacggact gatctcaaca tcgacaaaaa gatatgtacc
    12361 tctttcgtta attgagattg ccgacgcgcg catctccgga ttcaacggac gcacgtgccc
    12421 cccgcgaaga ttcgccccac gcaccaaaag cctgaccgga ctgatcgtat gtttggccca
    12481 ccatgaaggc ggatcgactt tcgtcaccat cggcgtttgc gcgtagaccg gcgaacaaga
    12541 accagccaca aaaaggcaca aaaagcacaa aagacgtaag gaaaaccttt tgtgcttttt
    12601 gtgccttttt gtggccagct tttggtgcct ttttgtggct gtcattagtt gtttggggtg
    12661 cgaggttttg cactatcgcc tgcagctacg accggcagcg gcggtaaagt ctcgccgtcc
    12721 ttcatcggtg ttagtggtgt cgcgccgtgc tggttaactt catccagacg cttgaactcc
    12781 gccagctctt tgtcagcctc gtcctttcgc ttcaatcgtg agtacgcgag aaagagttga
    12841 tagtgcacgc ccggatcctt cgcattcaat tcaatcccac gtgtaagtaa cggcactgcc
    12901 tcttcatatc gcttcaactt caccagcagc cgcccgagat cgtgatacgg cggaaagctc
    12961 ttcggatcga gctcgatcgc gcgtcttaat aaacgctcag cctcgacatt ttcgccacgc
    13021 tggctcgcga tgtaaccgag attcccaagc acatccggat tgtcaggctg cgacttgagc
    13081 gccttttgaa acgcctcaac tgccacgtca tactgtccct cagcacgcgc tgcgtagccg
    13141 agcagaa
//
LOCUS       DASSFQ010000091         6068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_35350, whole genome shotgun sequence.
ACCESSION   DASSFQ010000091 DASSFQ010000000
VERSION     DASSFQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35350"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFH96_02970"
     CDS             <1..342
                     /locus_tag="VFH96_02970"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HET6972955.1"
                     /translation="LFTLNWLDAQLTVLWIRLGVATEGNALMARILQHGDLTFLGAKL
                     AIGAFAAYILYRCAHLPIAKRGLNFVLGIYVILMLVHVATGCVALGWKTPIVVVDYLG
                     SLPNAFLSLFA"
     gene            550..1626
                     /locus_tag="VFH96_02975"
     CDS             550..1626
                     /locus_tag="VFH96_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6972956.1"
                     /translation="MILDRINNRFQPSQAAFSETPPAGISDPATSTDEQNNIEVYRTL
                     SPGVVFIHSTSYARDFFGFVEPQEGSGSGSILDQQGNILTNYHVIEGAQKLSVSMGGK
                     KDYAAEVVGGDPDTDLAVIRLTEKPAGGLTVVPMGDSDRLVVGQKVLAIGNPFGLDRT
                     LTTGVISGLQRPLRARNNRLIEGAIQTDASINPGNSGGPLLDSHGRMIGINSQILSPS
                     GSSAGVGFAVPVSIAKRVVPQLLQNGRVRRPKLGISTRDITSLRNQIDLPVDDGVLIY
                     QVARGGGAATAGLRGVQQTEMGDVELGDIIVGIDNDKVANSDDLFRVLDKHQIGDTVQ
                     VQIWRDGRRMSVPVRLMESPDSRR"
     gene            1648..1854
                     /locus_tag="VFH96_02980"
     CDS             1648..1854
                     /locus_tag="VFH96_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972957.1"
                     /translation="MHQTAHFFNLGYGWLCKHCSAEAAERKHHGHHHHGAQSPQGPAL
                     AKWVDEEHWRGLVCPRCGISERLA"
     gene            complement(1851..2498)
                     /locus_tag="VFH96_02985"
     CDS             complement(1851..2498)
                     /locus_tag="VFH96_02985"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HET6972958.1"
                     /translation="MNKQLFQAHPWHGVTPGPRTPELVNAYIEIVPTDAVKYELDKWS
                     GHLRVDRPQRFSSMSPTLYGFIPQTYCDREVAELCATRTGRTGIEGDKDPMDICVLSE
                     KSFTHGSFFLHARPIGGLRMIDGNEADDKIVAVLEADLAYGRITEIRDVPQGLIDRLK
                     HYFLSYKQLPSDKEKKVEIVDVYDRTEAQDVITRSLRDYEAEFGDADERGLKPRI"
     gene            2545..3600
                     /locus_tag="VFH96_02990"
     CDS             2545..3600
                     /locus_tag="VFH96_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972959.1"
                     /translation="MAASAEIITKPVASAISLRWIISARDDAIWFIGSVVSSYVLLIL
                     YVSGIVPLVPMVAAWAILIDAPHVFGTFSRTYFDRTERKNRARLLWGSLLFFAIGPVF
                     VLAGAGFVFFFVAALWAYYHLVKQHYGFMVLYKKKNGDLARVDNILDRGLLLFAFNYP
                     FVEFIARDPEAMVRVPSLLRGGVSGFSKLLLAGTIMFFLVWLGRQVQRAIAGEPLNVP
                     KYLLLAAAIPMHWIVLLTPMPHKPIAIVAILTIYHNFQYHRLIWFHNQKYANAGEKYG
                     AAELISRRLLFYIGFGILFGIIYQGPRQLLNNLGPGLATYLGISFLWGYAFVHYYLDS
                     KIWRVRRDPSVSKALNI"
     gene            complement(3629..5047)
                     /locus_tag="VFH96_02995"
     CDS             complement(3629..5047)
                     /locus_tag="VFH96_02995"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET6972960.1"
                     /translation="MEDKEIKELERAVEQIWEVARGFGLDPYPTNFEIVPATVMYEVG
                     SYALPGRYSHWTFGKTYHRMKTMYDFGLSKIYEVVINTNPAYGFLLETNSPIQNKMVI
                     AHVLGHVDFFKNNSYFSKTNRRMVESVSTHAQRMMEYEFKHGRKTVERFLDAVLAIEE
                     HIDPYFFIKRDISIAEEKRRAEHRVKDGPYDDLWLLDKSARVAKPPETIDDDKPRERL
                     PEKDLLYYLMKNSPSLAPWQRDAIAMVHEEMEYFVPQMQTKIMNEGWASIWHARIMRE
                     LDLSDNDHIEFANLHSGVVSPQKGQLNPYYLGFKIYEDIERRWNEPSKEEREKFGRAG
                     NEGREKIFEVRELDNDVSFLRNYLTEELCEELDLFVFELVEEEEWTITEKRWEKVRDQ
                     LVSNMTNFGFPYLQVEDGDYNGNRELYMKHNYEGTELDMRYARKALEHVFTLWGRPVH
                     LETVIDEESTVLHFNGDEHGED"
     gene            complement(5092..>6068)
                     /locus_tag="VFH96_03000"
     CDS             complement(5092..>6068)
                     /locus_tag="VFH96_03000"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HET6972961.1"
                     /translation="NYQKGKHVGQGDGKTRVGDVIGRESRPGSGQGTGQAGQDAGADY
                     YEAEVNIDELAALIFEDLHLPYLEEKAKKAVQSRKTQFNEIRRTGVIANLDKRRMILE
                     NIRRNAREGKARFGDIKKEDLRFKTWEEVMRYESNAVVIAMIDVSGSMGEFKKYIARS
                     FYFWMVRFLRTKYDNVEIVFISHHTEAKEVTEEQFFTQGESGGTVVSSAYQLALDIIK
                     QRYRPSDWNIYPFHFSDGDNYYSDNEEAVRLADELIATCNLFGYGEIGEEGSSSYRRS
                     SGALLSIFSDRLQHKDHFVGVRIDDKEDVYPALKQFFGKRDLAADERR"
BASE COUNT         1241 a         1701 c         1624 g         1502 t
ORIGIN      
        1 ctttttactc tcaattggct cgacgcgcag ttaactgtcc tctggattcg tctcggcgtc
       61 gcgaccgaag gcaatgccct catggcccgt atcctgcaac acggcgacct gacttttctc
      121 ggcgcgaaac tcgccatcgg agccttcgct gcttacattc tttatcgctg tgcccatttg
      181 ccaatcgcaa aacgcggcct caatttcgtg ctcggcatct acgtcatact gatgctcgtt
      241 cacgttgcga ccggctgtgt ggccctgggc tggaagacgc ccatagtcgt cgttgattac
      301 ctcggaagcc tgcccaacgc cttcctgtct ctcttcgctt aagagctttt gaaactcgcc
      361 gccgccgttc tacgttgata caatctgctc gcaacggcgc ccgttctgag agcatcctaa
      421 aagcgatgtt agccacctct tgcccaattt tagaaatact agagaggcac gacctttatg
      481 tataaaattt ccgcacgcca gattttaatg attgctctta tctcggggat ttttgcggcc
      541 ggagcagttg tgattttaga ccgcatcaac aatcgttttc agccgtcgca ggctgcgttc
      601 agcgaaactc cgccggcggg tatcagcgat ccggcgactt caactgacga acaaaacaat
      661 atcgaggttt accgcacact cagtcccggc gttgttttca ttcactcaac gtcttacgca
      721 cgcgacttct tcggcttcgt tgagccgcag gaaggctcgg gctccggatc gatcctggac
      781 cagcagggaa acatcctgac aaactatcac gtgatcgaag gcgcgcagaa actctcagtc
      841 agcatgggcg gaaagaaaga ctatgctgcc gaagtcgtcg gtggcgatcc tgacaccgac
      901 ctcgcggtga ttcgcctgac ggagaaaccg gctggcgggc ttactgtcgt gccgatgggc
      961 gattcagaca ggctggtcgt cggtcagaaa gtgcttgcga ttggaaatcc gttcggcctg
     1021 gatcgcacgc tgaccaccgg tgtgatcagt ggactacagc gaccactccg cgcgcgcaat
     1081 aaccgactga ttgaaggcgc gattcaaaca gatgcatcga tcaatcccgg taactccggt
     1141 ggaccgttgc tcgactcgca cgggcgcatg atcggtatca actctcagat tctttcgccg
     1201 tccggcagct cagccggggt cgggtttgcc gtgccggtga gtattgccaa gcgcgttgtg
     1261 ccgcagttgc tgcaaaacgg tcgggtgcgg cgtccgaagc tcggcatcag cacgcgcgac
     1321 attacgtcgc tgcgaaacca gatcgatttg ccggttgatg atggtgtgtt gatttaccag
     1381 gtcgcgcgcg gtggtggcgc agccacggca ggtttgcgtg gcgttcagca aaccgagatg
     1441 ggcgacgtgg agctgggcga tatcatcgtc ggtatcgaca acgacaaggt ggcgaattca
     1501 gacgatctct tccgagtact cgacaagcat cagatcggcg acacggttca ggtgcaaatc
     1561 tggcgcgacg gcaggcggat gtctgtgccg gtgcgtttga tggaatctcc tgactcaagg
     1621 cgctgatcgc atgtaaaatg cggctctatg caccagacgg cccacttctt caatttgggt
     1681 tatggctggc tctgtaaaca ttgcagcgcg gaggccgcag agcgcaaaca tcacggtcac
     1741 catcatcacg gcgcccaaag tccgcaaggc cccgcgctgg cgaagtgggt tgacgaagaa
     1801 cactggcggg ggctcgtttg cccccgctgc ggcatttccg aacggctcgc ctaaatccgc
     1861 ggttttaatc cgcgttcatc cgcgtcccca aactccgcct cgtaatcgcg taagcttctg
     1921 gtaatcacgt cctgcgcttc cgtgcggtcg taaacgtcca cgatctcgac cttcttctct
     1981 ttatcactcg gaagctgctt gtaactcagg aagtagtgct tcaagcgatc gatcaatccc
     2041 tgcggcacgt cgcggatctc ggttatgcgg ccataggcca gatctgcctc cagcaccgcg
     2101 acgatcttgt cgtcagcctc gtttccgtcg atcattcgca agccaccgat cggacgcgcg
     2161 tgcagaaaaa agctgccatg cgtaaacgac ttctcggaaa ggacgcagat gtccatcgga
     2221 tccttgtcgc cttcaattcc cgtgcggccg gtgcgtgtgg cacataactc ggccacctcg
     2281 cggtcgcagt atgtttgcgg tatgaagcca tatagtgttg gcgacatcga tgagaaacgc
     2341 tgcggacgat cgactcgcaa gtgccccgac cacttatcaa gctcatattt aactgcgtca
     2401 gtcggaacga tctcgatgta ggcgttgacg agttcgggcg tgcgcggccc cggtgttacg
     2461 ccgtgccatg gatgcgcctg gaagagttgt ttgttcatct cgacacgatg ataaaatggc
     2521 gcacgtccaa acgaaagcct ccttatggct gccagtgctg aaatcatcac caagccggtt
     2581 gcgagcgcga tctctttgcg ctggattatc agtgcgcgcg acgacgcgat ctggtttatc
     2641 gggtcagtcg tctccagtta tgtgctgctg attctttatg tgagcgggat cgttccgctc
     2701 gttccgatgg ttgcggcgtg ggcgatcctc atcgatgcgc cacacgtttt cggaacgttt
     2761 tcgcgcacct acttcgatcg cactgaacgg aagaatcgcg cgcggctgct ctggggctcg
     2821 ctgctgtttt tcgcgattgg accagtgttc gtactagccg gcgccggctt tgtctttttt
     2881 ttcgtggcgg cgttatgggc ctactaccat ctcgtcaaac agcactacgg cttcatggtg
     2941 ctttacaaga agaagaacgg tgatctcgcg cgtgtcgata acattctcga tcgcgggttg
     3001 ttgttgtttg cgttcaatta tccgttcgtc gagtttatcg cgcgcgatcc ggaggcgatg
     3061 gtgcgtgtgc cgtcgttgtt gcgcggcggc gtgagtggtt tttcgaagct gttgctcgcg
     3121 gggacgataa tgttcttttt ggtttggctt ggacggcagg tgcagcgcgc gattgcaggc
     3181 gagccgttga atgtgccgaa gtatctgttg ttagcagcgg cgattccgat gcactggatc
     3241 gtgttgctga cgccgatgcc gcacaagccg atcgccatcg tggcgatact cacgatctat
     3301 cacaacttcc agtatcaccg gctgatctgg tttcacaatc agaagtacgc gaatgcgggt
     3361 gagaaatatg gtgcggcgga gctgatcagc cggcgactgc ttttctatat cgggttcggg
     3421 attttgtttg ggatcattta ccagggaccg aggcagttac tgaataatct cgggccgggt
     3481 ttagcgacgt atctcgggat ctcgtttctg tggggatacg cgtttgttca ttactatttg
     3541 gattcgaaga tctggagagt gagaagggat ccgtcggtta gcaaagcgtt gaatatttag
     3601 ccacggatga cacggataaa atccggattt aatcttcgcc gtgctcgtcg ccgttgaaat
     3661 ggagcacggt tgattcttcg tcgatcacgg tctcgaggtg aacggggcga ccccagagcg
     3721 tgaagacgtg ttcgagggct ttgcgggcgt agcgcatgtc gagctcggtg ccctcgtagt
     3781 tgtgcttcat gtagagctcg cggttgccgt tgtagtctcc atcctcgacc tgcaaatacg
     3841 gaaaaccgaa attcgtcatg tttgaaacaa gctgatcgcg caccttctcc cagcgcttct
     3901 ccgtaatcgt ccactcctct tcctcaacca gctcaaacac aaacaagtcg agctcttcac
     3961 acaactcctc agtcaggtag ttgcgcagga acgagacatc gttatcaagc tcacgcactt
     4021 cgaagatctt ctctcgtcct tcattgcctg cacgaccaaa cttctcacgc tcttctttgg
     4081 acggctcgtt ccaacggcgt tcgatgtctt catagatctt gaagccgagg tagtacggat
     4141 tgagctggcc tttctgcggc gagacgacac cggaatgaag atttgcaaac tcgatgtgat
     4201 cgttgtcact gagatcgagc tcacgcatga tgcgcgcatg ccagatgctg gcccaacctt
     4261 cattcatgat cttcgtctgc atctgcggga cgaagtactc catctcctcg tgcaccatcg
     4321 cgatcgcatc gcgttgccag ggcgcgagtg acggcgaatt tttcatgagg taatacagca
     4381 gatccttttc gggcaaacgc tcgcgcggtt tgtcgtcgtc aatagtctca ggtggtttag
     4441 cgacccgcgc gctcttgtcg agcaaccaga gatcgtcgta tggcccatct ttcacgcgat
     4501 gctcggcgcg tcgcttttcc tcagcgatcg agatatcgcg tttaatgaaa aaatacggat
     4561 cgatgtgctc ttcgattgcc aacaccgcgt cgagaaatcg ctcaacggtc ttgcggccat
     4621 gcttgaactc atactccatc atgcgctgcg cgtgcgtcga gacactctcc accatgcggc
     4681 gattcgtttt tgagaagtaa gaattgtttt tgaagaaatc gacgtgaccc agcacgtgcg
     4741 cgatcaccat cttgttctga atcggactat tcgtctcgag tagaaacccg taagccgggt
     4801 tcgtgttgat aacgacttcg tagatcttcg agagtccgaa gtcgtacatc gtcttcatgc
     4861 gatggtaggt tttgccaaac gtccagtgcg aataacgacc cggcaacgca taggagccga
     4921 cctcatacat caccgtcgcg ggcacgatct caaaattcgt gggatacgga tcgagcccaa
     4981 acccgcgagc aacttcccag atctgctcga cggctctctc gagttccttg atctctttat
     5041 cttccataat attttagccg cggattaacg cgaatgaacg cagatctgcg tttatctgcg
     5101 ttcatccgcg gctaaatctc tcttgccaaa gaattgcttc aatgcgggat agacgtcttc
     5161 cttatcgtcg atccgcacac caacaaaatg atccttgtgc tgtaatcgat cgctgaagat
     5221 cgacagcaac gcgcccgaag aacgcctgta actcgacgat ccttcctcac cgatctcgcc
     5281 atacccaaaa agattgcacg tcgcgatcag ctcgtcagca agccgcactg cttcttcgtt
     5341 atcggagtag tagttgtcgc catcggagaa gtgaaacgga tagatattcc aatccgaggg
     5401 acgatagcgc tgcttgatga tgtcgagcgc gagctggtac gcggaagaaa cgaccgtgcc
     5461 gcccgattcg ccctgcgtaa agaactgctc ctccgtcacc tctttagctt ccgtgtgatg
     5521 cgagatgaaa acgatctcga cgttgtcgta cttcgttctc aaaaaccgca ccatccagaa
     5581 gtaaaaactg cgcgcaatgt atttcttaaa ctcgcccatc gaacccgaaa cgtcgatcat
     5641 cgcaatgaca accgcgttcg attcgtagcg catcacttct tcccacgtct tgaatctcag
     5701 atcctctttc ttgatgtcgc cgaagcgcgc cttgccttcg cgcgcattgc gacgaatgtt
     5761 ttcgaggatc atgcggcgct tgtcgaggtt tgcgatcaca cccgtgcgac gaatctcgtt
     5821 gaattgagtc ttgcgcgact gcaccgcctt cttcgctttc tcttcgagat acggcaggtg
     5881 aagatcttcg aagatcagtg cggccagctc gtcaatgttt acttccgctt cgtagtaatc
     5941 cgcgccggcg tcctgccctg cctgacctgt gccctgacca ctgcccggcc ggctctcgcg
     6001 tccaatcacg tcgccaacgc gcgtctttcc gtcgccttgg cccacgtgtt tacccttttg
     6061 atagttga
//
LOCUS       DASSFQ010000092         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2122907, whole genome shotgun sequence.
ACCESSION   DASSFQ010000092 DASSFQ010000000
VERSION     DASSFQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2122907"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFH96_03005"
     CDS             complement(<1..839)
                     /locus_tag="VFH96_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972962.1"
                     /translation="MKLLLRSAFIRVHLRLTILAFFAIVGPITVRADLNQKQARKAIQ
                     TIGGWSLPGSAVRLQIRSNSAESAEVSAEIQAVFRMRLYEGHWQLSDIRSAPDRWERL
                     EIIARAAKVDLPGDECDAPPQFARTKFATELTTKRARCFVASLLGVALPSDDVRIKDI
                     DVFGFSIGSESSALLTALIRVDFRFARDKSGWQVTEFKSGRRDWVNIASLPAAINAAK
                     RATASDDLSTIARALGEYRRDRGYFVVSDKESVLIDHLSPRYLARVIRVDPWHRPYRY
                     DGQ"
     gene            complement(847..1260)
                     /locus_tag="VFH96_03010"
     CDS             complement(847..1260)
                     /locus_tag="VFH96_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6972963.1"
                     /translation="MENNNLTNGTEDRRMLVADDDPAILRLIATILEKENFSVVTARD
                     GREAYKILQADPNFTAAILDVVMPHISGPELVRYMKSEERLRKIPVMMMTAEQDPKLS
                     SDSFAAGAIVFLPKPFTTAQLQIMLQMLIGKKTAE"
     gene            complement(1280..2347)
                     /gene="pdxA"
                     /locus_tag="VFH96_03015"
     CDS             complement(1280..2347)
                     /gene="pdxA"
                     /locus_tag="VFH96_03015"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET6972964.1"
                     /translation="MRHSEQSSTNPGPRPRRVLPRIGITMGDPAGIGPEVVLKAVAED
                     EIKRVCVPVIIGDAPLLAHTARTLDLQSGYDIIRTGESFPDQLSEPVIYHLDNISGLI
                     EPGIESGAAGKAAAGYIEAAVELCAAGSIDAIATAPINKRALFLGGYSFPGHTEFLAH
                     LTGAEEYAMAFVAANLRIVLLSTHVPLSEAIRLVERDRLIKVINLTHRELQRWGIERP
                     RLAVAALNPHGAEGGLFGVEEASEIMPAIESAQRLDDIDVHGPFSADTVFLRASRGEF
                     DAVVACYHDQAMIPVKCLSFGEAVNVTLGLPFIRTSVDHGTAFDIAGKGLAEHSSMVA
                     AIKLAADLSTQAGESCRAVEV"
     gene            complement(2419..>2665)
                     /locus_tag="VFH96_03020"
     CDS             complement(2419..>2665)
                     /locus_tag="VFH96_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972965.1"
                     /translation="KKRVPPPNETNAEIFYYKKQIDAHTLMVLVLLDGEEIEGTIEWY
                     DRGALKVNRKGAPNLLVLKRNIKYIYKADERVESASA"
BASE COUNT          606 a          780 c          692 g          587 t
ORIGIN      
        1 tgttggccgt cgtatcgata gggacggtgc catgggtcga cgcggatcac gcgcgccagg
       61 tagcgcgggc tgaggtgatc gatcaggacg gattctttat cggaaactac aaaataaccg
      121 cggtcgcggc gatattcacc gagcgccctc gcgatggtag aaagatcatc agacgcagtc
      181 gcgcgcttgg ccgcattaat cgctgcggga agactcgcga tgttcaccca atcacgccta
      241 ccgctcttaa actcagtcac ctgccagccg ctcttatcac gcgcaaaacg gaaatctacg
      301 cgaatcaacg ccgtcaacag tgccgacgat tcagatccaa tcgagaaacc aaacacatcg
      361 atatctttga tccgcacatc gtccgaagga agcgcgacgc caagcaaact cgccacgaaa
      421 caacgagctc gtttcgtcgt caactccgtc gcaaacttcg tgcgggcaaa ctgcgggggc
      481 gcgtcacatt cgtctcctgg caaatcgacc ttcgcggcgc gggcaatgat ctccaatcgt
      541 tcccaacgat cgggcgcgct tctgatatcc gataactgcc agtgaccttc gtacagtcgc
      601 atccgaaaga cggcctgaat ctcggcgctg acttccgcgc tctccgcact gttcgatcgg
      661 atctgaagtc gaaccgcgct accgggcaat gaccagccgc cgatcgtctg gatcgctttg
      721 cgcgcttgtt tctgattgag atctgcgcgg accgtaattg gaccaacgat cgcgaagaag
      781 gcaagaattg taagccgcag atgaacgcgg ataaacgcgg atctgagtaa cagtttcatc
      841 agcaacctac tcggcagtct tcttaccaat caacatctgc aacatgatct gcagttgcgc
      901 ggtcgtgaac ggcttcggca aaaagacgat cgcgccggct gcgaaactgt cagacgacaa
      961 cttcggatcc tgttccgcgg tcatcatcat caccgggatc tttcgcaggc gctcttcgga
     1021 tttcatgtag cgcacgagct cgggaccgga aatgtgcggc atgacaacgt cgagaatcgc
     1081 cgccgtaaaa ttcggatccg cctgaagaat tttgtaagcc tcgcgcccgt cacgcgcggt
     1141 cactacactg aaattctctt tctccaaaat cgtggcgatc aatcgcagaa ttgccggatc
     1201 gtcatccgcc accagcatcc gccggtcctc ggttccgtta gtaaggttgt tgttctccat
     1261 gtgagtgttc ttctctagtt taaacttcta ctgcccgaca gctctcgccc gcttgcgtgg
     1321 aaagatctgc agccagtttg attgctgcca ccatgctcga atgttcagcg agccctttgc
     1381 ccgcaatgtc aaaagccgtg ccgtggtcga cggacgtcct gatgaatggt aaaccaagtg
     1441 tcacgttaac ggcttcacca aacgacaaac actttaccgg aatcattgcc tgatcgtgat
     1501 aacacgcaac cactgcatcg aactcgccgc gcgaagcacg caggaataca gtatcagccg
     1561 agaatggacc atgcacgtcg atatcgtcga gccgctgcgc cgattcgatc gccggcataa
     1621 tctctgaagc ctcttcaact ccaaacaatc cgccttccgc tccatgcgga ttcagggctg
     1681 cgactgccaa gcgcgggcgc tcgatgcccc accgctgaag ttcacgatgc gttagattga
     1741 tcaccttgat cagccgatcg cgctcaacca gacgaatcgc ttcagagagt ggcacgtggg
     1801 tcgacaacaa cacgatgcga aggttggcgg cgacaaacgc catcgcatac tcctcggcgc
     1861 cagtcagatg agcaagaaac tccgtgtgac ccggaaaact gtagccgccg agaaacaacg
     1921 ctcgtttatt aatcggcgcg gtcgcgatcg catcgatgct tccggcagca cacaactcca
     1981 ctgccgcttc gatgtaaccc gcggcagctt tacctgcggc gcctgactcg atgccgggtt
     2041 cgatcaaacc actgatattg tcgagatgat aaatgaccgg ttcggaaagc tgatcgggga
     2101 acgactctcc cgtgcgaatg atgtcgtagc cggattgaag atcgagagtg cgtgccgtgt
     2161 gcgcgagcaa tggagcatcg ccgataatca cgggcacgca aactcgtttg atttcatctt
     2221 ccgcaacggc tttgagaacc acttccggtc caataccggc agggtctccc atcgttatgc
     2281 cgatgcgcgg caaaactcgc ctgggacgag gaccaggatt ggttgagctt tgttctgagt
     2341 gtcgcattgc ttgaggcgac gcactatttc agcgagaact gtgggcgagg cagtacagtt
     2401 tcgctctcga ttcccctttt aggcgctggc tgattcaacc cgctcgtcgg ccttgtagat
     2461 gtacttgata ttccgtttga gcacgagcag attcggggcg cctttgcggt ttaccttcag
     2521 cgcaccacga tcgtaccact cgatagtgcc ttcaatttcc tcgccgtcaa gcagcactag
     2581 caccatcagt gtatgagcgt cgatctgttt cttgtaataa aagatctccg cgttagtttc
     2641 gttgggaggc ggaactcttt tcttt
//
LOCUS       DASSFQ010000093         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1228931, whole genome shotgun sequence.
ACCESSION   DASSFQ010000093 DASSFQ010000000
VERSION     DASSFQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228931"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..966
                     /locus_tag="VFH96_03025"
     CDS             202..966
                     /locus_tag="VFH96_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET6972966.1"
                     /translation="MKAELQKLIALQNLDTTIRKLEKEQKAIPERRAEIEGEFDQRAF
                     EIRALENRRDEAKHTRARLENEVMEQKGRAERAERNLMSSKKPDEYTAAIREADAARK
                     QISGLETQILEQMEQLEQAEGSLKERADEVASMNSDREAKLKAFDDETGTIGDRLATA
                     RKEREEVFASLPKPMSAQYSRIGARIRDGVAVAEARNRSCSACFMSLRPQMMAEIRRG
                     DEIITCDNCGRILYVAPPAEPVKADAATTQPNVAVS"
     gene            991..1965
                     /gene="galE"
                     /locus_tag="VFH96_03030"
     CDS             991..1965
                     /gene="galE"
                     /locus_tag="VFH96_03030"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET6972967.1"
                     /translation="MRVFVTGGAGYIGSVVTEELVNDGHEVVVYDNLSKGHRGAVVDG
                     ARFVEGDLFEADKLRQTLNDNRIDAVIHMAASSLVGESVEQPAKYYQNNVVAGLVLLD
                     AMRDCGVRRIVFSSTAATYGEPEAQPIFESAPTNPTNTYGETKLAFERAMHWYEKAHG
                     LRYASLRYFNAAGASEKCGEDHDPETHIIPITLQAATGKRAHVEIFGDDYPTSDGTCI
                     RDYIHVIDLARAHILALDVLSERSAIYNLGCGGEGYSVRDVIETAKRVTGKEIKVRMG
                     PRRAGDPAVLIASSDKIKSELGWQPQFQDLGVIIESAWKWMVNHGSYG"
     gene            complement(1978..2583)
                     /locus_tag="VFH96_03035"
     CDS             complement(1978..2583)
                     /locus_tag="VFH96_03035"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6972968.1"
                     /translation="MRITLLFLILVAFIAAPPQKVTYNLDASQSKFMAHANRSGLLWF
                     KGKSHHLAASDFSGQVELTPDTITPASLRLVVKAASLHETGADFTEPQKQIIDKELKD
                     IVLHPDQYPDITFQSTNVTAKGLGGGRYDVKINGNLTLHGVTKPVMIPATVTLNGSTL
                     RAVGEFSIDRDDYGVKATSAFHGMVRVDDDIKFEFDIVGRR"
     gene            2598..3563
                     /gene="ligD"
                     /locus_tag="VFH96_03040"
     CDS             2598..3563
                     /gene="ligD"
                     /locus_tag="VFH96_03040"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET6972969.1"
                     /translation="MTKDEVKRTKQKSGTVVTVEEFLRLDRPKGDLIIKAGREQVAVT
                     SLERIYWPDEKLTKFDLLSYYLKVADYIMPYLQDRPAILQRYPRGIKAPMFFQQDLES
                     APEFIKTARLTNQEGRQLDYGVYSTIGSVLHFVTLGTIEQHPWHSTIKRLDKPDWIAI
                     DLDPKKAPWENVLQVALVVKEVADEIGLKAFPKTSGSSGIHIYVPLKPANEYEKVAEF
                     SRLFATEVARRAPQIATVERTIAKRKSTQVYVDWMQNARGKSLAAVFTARAKPKAPVS
                     MPLTWPQIAKGVKIDDFTIVNVPELLKKKGDAWAEFFSSRQSLKF"
BASE COUNT          879 a          925 c         1082 g          745 t
ORIGIN      
        1 cgagattcca accgtgccaa cggtcaatat cgagatgccg cgcattcgcg tcatgccgcg
       61 catcagggtc tcgcgaggcg tttacggccc gatctgaacc cttcagtgac accgcttgca
      121 ccaactactc ggattgtgtg atgatcgcaa gcgatgggaa tcgtcataaa cagccctgat
      181 cttttgagga gagaataaag agtgaaagcg gaattgcaga agttaatcgc tttgcagaat
      241 cttgatacga ccatacgaaa actagaaaaa gagcaaaaag cgataccaga aaggcgcgcc
      301 gagatcgaag gagagttcga tcagcgcgcc tttgaaatta gggcgctcga aaaccgccgc
      361 gacgaagcca aacacacgcg agcccgtctc gaaaacgaag tcatggagca gaaaggccgc
      421 gctgagcgag ccgagcgaaa cctgatgtcg tcgaagaagc cggacgaata caccgccgcg
      481 attcgtgagg cagatgcagc gcgcaaacag atctcaggcc tggaaacaca gatcctcgaa
      541 caaatggagc aactcgaaca agccgaagga tctttgaagg aacgtgctga cgaagtcgcc
      601 agcatgaatt ccgatcgcga ggcgaagctg aaagcgtttg acgacgagac tggcacgatc
      661 ggtgaccgtt tggctactgc tcgcaaagaa cgcgaagaag tgtttgcgag tctgccgaag
      721 ccgatgagtg ctcaatattc gagaatcggg gcgcgcatcc gcgacggagt ggcagtggcg
      781 gaggcgcgca atcgttcgtg cagcgcgtgt ttcatgtctt tgaggccgca gatgatggcc
      841 gagatccggc gtggggacga gatcattacg tgtgataact gtggtcgcat cctttacgtc
      901 gcaccgcctg cagaaccagt caaggcggat gcagcgacga cacagccgaa cgttgcggtc
      961 agctagcgaa aaattatcaa ggggtcattc atgcgggttt tcgtaacggg cggcgccgga
     1021 tacatcggta gtgtcgtcac tgaagaactg gtaaacgatg gccacgaagt tgtggtctac
     1081 gacaatttat ccaaaggaca caggggcgcc gttgttgacg gcgcccgttt tgtcgaaggc
     1141 gatctttttg aagcagacaa gcttcgccaa acgctgaacg acaatcgcat cgatgcagtc
     1201 attcacatgg ctgcgtcgtc gctcgtcggt gagtcagtcg agcagcccgc gaagtattat
     1261 caaaacaacg ttgtcgcggg attggttttg ctcgatgcga tgcgcgactg tggggtccgt
     1321 cgcatcgtct tttcttcaac ggcagcgacg tatggcgagc ccgaggcgca gccgatcttc
     1381 gaatcagcgc cgaccaatcc caccaacact tacggcgaaa caaaactcgc attcgaacgc
     1441 gcgatgcact ggtatgagaa agcgcacggg cttcggtatg catcgttgcg ttatttcaat
     1501 gctgcgggag cgagtgagaa gtgtggtgag gatcacgatc cggagacgca catcattccg
     1561 atcacattgc aggcagcgac cgggaaacgc gctcacgtcg agatctttgg cgacgactat
     1621 ccgacgtctg atgggacgtg cattcgcgac tacattcacg tgatcgatct ggcgcgcgca
     1681 cacattctcg cgctcgacgt tttgagtgag cgcagcgcga tctacaacct cggatgcggt
     1741 ggcgagggat acagtgtccg cgacgtgatc gagacagcga agcgggttac cgggaaagag
     1801 attaaggtgc ggatgggacc acggcgcgca ggtgatccgg cagtgttgat tgcaagctca
     1861 gacaagatca agagtgagct cgggtggcag ccgcagtttc aggatctggg cgtgattatc
     1921 gagtcagcgt ggaagtggat ggttaaccac ggatcatacg gataaaaagc acggatttta
     1981 tctgcgtccc acgatgtcga attcgaattt aatgtcgtcg tctacgcgca ccattccgtg
     2041 aaaggctgat gttgccttca ccccatagtc atcgcgatcg atgctgaact cgccaaccgc
     2101 acgcaacgtg ctcccattca gcgtgacggt tgccgggatc atgacgggct tcgtgacgcc
     2161 atgcagcgtc aggttgccgt tgatctttac gtcgtatcga ccacccccca aaccctttgc
     2221 agtcacgttc gtgctctgaa aagtgatgtc cggatactgg tccgggtgca gcacgatatc
     2281 cttcagctct ttatcgatga tttgcttctg cggttcggtg aagtcagcgc cagtctcgtg
     2341 cagcgatgcg gctttgacga cgagtcgcag cgatgcgggc gtgatcgtgt caggcgtaag
     2401 ctcgacctgc ccgctgaaat cacttgcagc aaggtgatgg ctcttaccct tgaaccacag
     2461 cagtccgctg cggttggcgt gggccatgaa cttgctctgg ctggcgtcga gattgtaggt
     2521 taccttttgc ggtggtgctg cgatgaatgc gacgagaatc aggaacagta atgtaattct
     2581 catggtccga atttagcatg actaaagacg aagtaaaacg aacgaaacag aaaagcggca
     2641 cggttgttac agttgaagaa tttctgagac tggatcggcc caaaggcgac ttaattatca
     2701 aggccgggcg cgagcaggtc gcagttacga gcctcgagcg tatttactgg cccgatgaga
     2761 aactcacgaa gtttgacctg cttagttact acctgaaagt cgcggattac atcatgcctt
     2821 acttgcagga ccggcccgcg attctgcaac gctatccgcg cggcatcaag gcgccgatgt
     2881 tctttcagca ggacctcgaa agcgcacctg agttcatcaa gacggcgcgg ctcacgaatc
     2941 aggaaggccg gcaactcgac tacggagttt attcaacgat cggatcggtg ctgcacttcg
     3001 tgacgctcgg cacgatcgaa cagcatccgt ggcactcaac gatcaaacga ctcgacaagc
     3061 ctgactggat cgcgatcgat ctcgatccga aaaaggcgcc gtgggaaaac gtgttgcagg
     3121 tggcgctcgt cgtgaaggaa gtcgcggatg agatcggatt gaaggcgttt ccgaagactt
     3181 ccggatcgtc gggcattcat atctacgtgc cgctcaaacc ggcgaacgag tatgagaagg
     3241 tggcggagtt ttcgcgactg ttcgcgacgg aagtggcgcg acgtgcgccg cagatcgcga
     3301 cggtcgagag gacgattgcg aagcggaaga gcacgcaggt ttacgttgac tggatgcaga
     3361 acgctcgcgg caagagtttg gcagcggttt tcacggcgcg tgcgaagccg aaggcgccgg
     3421 tatcgatgcc gctgacgtgg ccgcagatcg cgaagggtgt aaagatcgac gacttcacca
     3481 tcgtaaacgt accggagctg ctaaagaaaa agggcgacgc gtgggctgaa ttcttctcat
     3541 cgcgccaatc tctaaagttt taaagtagcc gcggatttac gtgtccgaca tgctttagct
     3601 tgtcggtcat tccgacaagc taaagcatgt c
//
LOCUS       DASSFQ010000094         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1527367, whole genome shotgun sequence.
ACCESSION   DASSFQ010000094 DASSFQ010000000
VERSION     DASSFQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527367"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1889
                     /locus_tag="VFH96_03045"
     CDS             72..1889
                     /locus_tag="VFH96_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6972970.1"
                     /translation="MSTAVKQYHEEEAIGKTYDLQVARRLLRYLKPYRRLLTAALALT
                     LVLNLLGVLQPKFTEYAIDWYIVPRKTDGLTLFVVLYLVSQLLRLVFSYFQTMLVESI
                     GQYVMFDIRKELYSKLQNQEVAYYDRNPVGRIMTRLTTDVDSLNQLFTDGVTDLLGDL
                     VMIGAIITVMAWTDPKLTLISLLTVPMLFAATTWFRRGARKGYDLVRTRIARINAFLQ
                     EHFAGAQTVQIFNAETKSLSKFDHINEQYRKANIDTIFYYAVFFPLVDLIGAIGIALI
                     IWYGGYRVMQNTPAHTVLTLGALVAFIQYSQQLFQPIRDISDKYNILQAAVVASHRIF
                     KTLDQPIAVLSPEQPLKAERARGRIEFQNVWFAYQDEDWVLKDVSFTVEPGQSIALVG
                     HTGSGKTTITNLLMRFYDIQRGRILLDGVDLRDWDLRALRKNFAVVLQEVFLFSGSIE
                     GNIRLGREDINEDRVRWAAQEVHADSFIRRLKGEYEAEVRERGAGLSVGQKQLISFAR
                     ALAFDPAILILDEATSSIDTETEQLIQRAIERVMRDRTSIVVAHRLSTVQNADRIIVL
                     HHGEIREQGTHQELLAERGLYWKLYKLQYADSRSVLATD"
     gene            complement(1922..3172)
                     /locus_tag="VFH96_03050"
     CDS             complement(1922..3172)
                     /locus_tag="VFH96_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972971.1"
                     /translation="MNTRTRLALFVVAVLFAAVEMKAQESAVRPRVVTPVTQEKQAPS
                     LPTAAPQAKPADLGPTLPSPEMDAQKKVAAPITHLTPSIIQGRISEAQRTFKTRPKPT
                     AMAVGQIDFVTIAALDRDTARTHFITVLKKTFLTKGSVINTTTSEGASVTVNIVRANG
                     VNTAMTIFTPDGKSLAPLTIEYPIERGGKFLETAYYTSAHPALLSPDLVRSGQGYVRN
                     MLDLAAKRLRDKGVAISPMLVDVAERLCVVEHVDHQRFREENRIALYDEIYSLFALNQ
                     IDTYRFSVSTAGAGGMVQMIPWAYNLERERYPGVGLIPDFVTGMRNHNNALQAMLLYM
                     HDTWNEMTANDEVKYALNSKTATPSELLAAGYNSNAAKLPGYLKRGGDNWRVLIPRET
                     QMYLQIYKSFESLVPMQTRDADKR"
     gene            3417..>4197
                     /locus_tag="VFH96_03055"
     CDS             3417..>4197
                     /locus_tag="VFH96_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972972.1"
                     /translation="MIKTNDYPAARKSDQTDDYHGVKVADPYRWVEDLDSAETRAWVE
                     AENKLSFGFLESIPERTAIKERLTKLWNYEKYGIPFREGNRYFYTRNSGLQNQAVLYT
                     VTSLDAQPQQVLDPNTLSADGTVALSGLQVSPDGKLLAYSLSASGSDWQEWKVRDVET
                     SKDLSDDLKWVKFSGASWTRDGKGFFYSRYDEPKSDALKGTNYFQKVYYHKLGTPQSE
                     DVLVYERPDQKDWLFNGTVTEDGNYLIITVFQGTDVKSRIYY"
BASE COUNT          923 a         1098 c         1198 g          978 t
ORIGIN      
        1 aaaagccatc cacatattac acagatcaac ctctgtgtaa tctgtgcaat ctgtggatgg
       61 atctgaaata gatgtctact gcggtaaagc aatatcacga ggaagaggcg atcgggaaga
      121 cttacgatct tcaggtggcg cggcggctgc tgcgttatct caagccttac cggcgtcttt
      181 taacggccgc gcttgcgctc actctcgtgc tgaacctgct cggagttttg cagccgaaat
      241 tcacagagta cgcgatcgat tggtacatcg tgccgcgaaa gactgatggg ctgacgctgt
      301 tcgtcgtttt atatctggtg tcgcagctct tgcgtctggt cttctcttac tttcagacga
      361 tgctcgttga gtcgatcggg cagtatgtga tgttcgatat ccgcaaggag ctttacagca
      421 aactgcagaa ccaggaagtc gcgtattacg atcgcaatcc agtcggccgc atcatgacgc
      481 gactgactac agacgtcgat tcactcaacc agctattcac cgacggcgtc accgacctgc
      541 tcggcgatct cgtgatgatc ggcgcgatca ttaccgtgat ggcgtggacc gatccgaagc
      601 tcacgctcat tagtttgctg acggtaccga tgctgtttgc tgcgacgacg tggtttcgac
      661 gtggcgcacg caaggggtac gatctcgtcc gcacgcgcat cgcccgtatc aacgcatttt
      721 tgcaggaaca ctttgccggc gcgcagacgg ttcagatctt caacgcggaa actaagtcgc
      781 tgagcaagtt cgatcacatt aacgaacagt atcgaaaagc gaacatcgac acgatctttt
      841 attacgcggt cttctttccg ctcgtcgatc tgatcggcgc gatcggcatc gcccttatca
      901 tctggtacgg cggttatcgg gtgatgcaga acacgccggc ccacacggtg ctcacactcg
      961 gcgcgctcgt cgcgttcatc cagtactcgc agcagttgtt tcagccgatt cgcgacatct
     1021 ctgacaagta caacatactg caagctgcgg ttgtcgcttc gcatcgcatc ttcaagacac
     1081 tggaccagcc gattgcagta ttgtcgccgg agcagccgct caaagccgag cgtgcgcgcg
     1141 gtcgcatcga gtttcagaat gtttggtttg cttaccagga tgaagactgg gtgttgaagg
     1201 acgtgtcgtt cacggtcgag ccgggacagt cgatcgcgct cgtcggtcac actggttcag
     1261 gcaaaactac gatcacgaat ttgttgatgc gtttttacga tattcagcgt ggacgaattc
     1321 tgctcgacgg tgtcgatctg cgcgactggg atctgcgggc gctgcgaaag aactttgcgg
     1381 ttgtgcttca ggaagtgttt ctgttcagcg ggtcgattga agggaacatt cgtctcggtc
     1441 gcgaagacat caacgaagat cgtgtgcgct gggcggcgca ggaagttcat gctgatagtt
     1501 ttattcgtcg actgaagggt gaatacgagg ctgaggtgcg tgagcgtggc gcggggttat
     1561 cggtggggca gaagcagttg atctcgttcg cgcgagcgct cgcgtttgat ccggcgattc
     1621 tgatcctcga cgaagcgacg agctcgatcg ataccgaaac cgagcagtta atccaacgcg
     1681 ctatcgagcg cgtgatgcgt gatcgcacat cgatcgtggt cgcccacagg ctctccacag
     1741 ttcaaaatgc ggaccggatc atcgtcctgc atcacggtga gattcgcgag cagggaacgc
     1801 accaggaatt gctggcggag cgtggtctct actggaagct ttataaacta cagtacgcgg
     1861 attctcgatc agtattagcc accgattaac acggaaaata cggctcgatc cgtgttttta
     1921 atcagcgttt atccgcgtcc cgggtttgca tcggcacgag gctttcgaac gatttgtaga
     1981 tctgcaggta catctgcgtc tcgcgcggaa tgagtacgcg ccagttgtcg ccgccacgtt
     2041 tcagatagcc cggcagcttg gcagcatttg agttgtaacc cgccgcgagc aattcactcg
     2101 gcgtcgcggt cttcgagttg agcgcgtact tcacttcatc gttcgcagtc atctcgttcc
     2161 aggtgtcgtg catgtagagc agcatcgcct gaagcgcgtt gttgtgattg cgcatccccg
     2221 tcacaaaatc agggatgaga ccgacgcccg gataacgctc gcgctcgagg ttgtaagccc
     2281 acgggatcat ctgcaccatg ccgcccgctc ccgccgtgct cactgaaaaa cgatacgtat
     2341 cgatctgatt gagcgcaaac aacgaataga tctcatcgta aagcgcgatt cggttctcct
     2401 cgcggaagcg ttggtgatcc acatgttcga ccacgcacag acgttcagcc acatcgacga
     2461 gcatcggcga gatggctaca cccttgtcgc gcaaacgctt cgcagccaag tcgagcatgt
     2521 tgcgcacgta accttgtcct gagcgcacga gatccggtga aagcagagcc ggatgtgccg
     2581 aagtgtaata agccgtttcg agaaactttc ctccacgctc gatcggatat tcgatcgtga
     2641 gcggcgcgag tgatttgccg tcaggcgtga agatcgtcat cgcggtgttg acgccgttcg
     2701 cacggacgat gttgaccgtg acgctcgccc cctcagatgt cgtggtattg atcacagagc
     2761 ctttggtcag aaaagtcttc ttcaaaaccg tgatgaagtg tgtgcgcgcg gtatctcgat
     2821 cgagggccgc gatcgtgacg aaatcgatct gtccgacagc catcgcggtt ggcttcgggc
     2881 gcgttttgaa cgtgcgctgc gcttccgaga tgcggccttg aatgatcgac ggcgtcaggt
     2941 gcgtgatcgg cgcagcgacc tttttctggg cgtccatttc tggcgaaggc agcgtcggtc
     3001 cgaggtcagc cggcttggcc tgcggcgccg ccgttggcag cgaaggcgct tgcttttcct
     3061 gagtaacagg ggtgacaacg cgtgggcgaa ctgcgctctc ctgagccttc atctcgacgg
     3121 cagcaaaaag aacggcgacg acaaataggg cgagacgggt acgggtattc atatgtttta
     3181 tctcaggaag agttacagga ctaaagccac gacgcgtcga ggggcgacaa cgtggcgtta
     3241 gattcgcgca gactcaaggg agttgtcaac ctctttttgg ccgcaaaaag gcataagaag
     3301 cgcaaaattt gtatactgtc gggttcgaaa ctagataggg agcgtttgtt atgtcttttt
     3361 acccacccat tcgctttgct gtcattgtgt gtttagtggc aggtataacg cagcagatga
     3421 ttaaaacgaa tgattacccc gccgctcgaa agagcgatca gaccgacgat tatcacggcg
     3481 ttaaagtagc cgacccgtat cgctgggtgg aagatctcga ttcagcggag acgcgcgcat
     3541 gggtcgaggc tgagaataag ctctcgtttg gttttctgga gtcgattccg gagcgcacgg
     3601 cgatcaaaga gcgcctgacc aagctctgga actatgagaa gtacggcatt ccgtttcggg
     3661 aaggcaatcg ctatttctac acgcgtaaca gcggcttgca gaatcaggcc gtcctctaca
     3721 cggtgacttc gctcgatgcg cagccgcagc aagtgctcga tccgaataca ctttcggctg
     3781 atggaacagt tgcgctctcc ggtttgcaag tcagtcctga cggaaaattg ctggcttaca
     3841 gtttgtcagc gtctggttcc gactggcagg agtggaaagt tcgcgacgtc gagacgagca
     3901 aagacctttc agatgatttg aagtgggtga agttttccgg agcttcatgg acgcgtgacg
     3961 gcaagggctt cttttacagc cgttacgatg agccgaaaag cgatgcgctc aaaggcacga
     4021 actactttca aaaagtctat taccacaaac tcggcacgcc gcagtcagaa gacgttctcg
     4081 tctacgagcg tcccgatcag aaagactggt tattcaacgg cacggttact gaagacggca
     4141 actacctgat catcaccgtc tttcagggca cggacgtgaa gagccgtatc tactaca
//
LOCUS       DASSFQ010000095         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1229738, whole genome shotgun sequence.
ACCESSION   DASSFQ010000095 DASSFQ010000000
VERSION     DASSFQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229738"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFH96_03060"
     CDS             complement(<1..698)
                     /locus_tag="VFH96_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6972973.1"
                     /translation="MNLEIFHPAVARWFARSFPAPTDPQLQAWPEIKKQRHTLIAAPT
                     GSGKTLAAFLSAIDDLVRLGVEGNLDDSTHVIYVSPLKALSNDVQRNLQVPLEGIQEE
                     LKALGLPEVNIRTLVRTGDTPASERTAMTKRPPHIVVTTPESLYILLTSEGGRRMLQT
                     ARTLIVDEIHAVVGDKRGSHLALSIERLEQLVRSNNASLTRIGLSATQRPIEEVARFL
                     VGTENLDASNTPNCA"
     gene            complement(803..1450)
                     /locus_tag="VFH96_03065"
     CDS             complement(803..1450)
                     /locus_tag="VFH96_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HET6972974.1"
                     /translation="MTTFLTDIETRVLGSLIEKQVTTPEYYPLTLNALTLACNQKNNR
                     NPVTTYPENQVADAVESLREKNLAYVFYGSTSRVPKYKHVMPEVMHLTHPEVALMCAL
                     MLRGAQTLGELRGNAGRLHDFASLEEIETTLNGLISHDSEPLVARLPRQPGQKEGRFA
                     HLLNGEIDLEALAETERVAAASPSRRAGLEQKVDELAAEVEKLKEQFEQFRKQFE"
     gene            complement(1519..>2976)
                     /locus_tag="VFH96_03070"
     CDS             complement(1519..>2976)
                     /locus_tag="VFH96_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972975.1"
                     /translation="DMLLDTQYYNVSDPAPYDDTGWTMGAMRNVKTVRVVDKSVLDAP
                     ATLLTQDAKVTGQLAGPASAVAYLINHNTDNTLATLRFRLKDVKMSAAEDSFKVGDQQ
                     YNTGSFIIKKDGNPADLRARLESAATELGLKAVGVDKLPEVKTHELAVPRIAIVHTWI
                     NTQNEGWYRIEFDKYQIPYTYISDQVIGNTPNLREKFDVLIFPPVGGSAQTIVNGMPK
                     RGDPIPWKASAVTPNFGNSPDQTDDMRGGMTVAGVSNLQKFIEDGGLFITIGGVSQVP
                     IDYGITTGVSIQPADKLQARGSIYNSTFADRKSPITYGYDAGLPIYFSQSPLFQVQTG
                     GGPGGGGGGAASRASGRGGLNDPDVIQAMPQPRPADPNNRPDPDADLRESPFFVPPQL
                     RPRVVLRFASDEKNLLISGMLAGGNELANRPAIVDVPVGRGHVVMFATNPMWRHQTQG
                     EFFLVFNAALNYDNLNVGRSEPRGQRPSTADGDDQ"
BASE COUNT          585 a          857 c          862 g          672 t
ORIGIN      
        1 gcgcagttgg gtgtgttgct cgcatcgagg ttttcagtgc cgacgagaaa gcgcgcgact
       61 tcttcgatcg gacgctgcgt tgctgagagc ccgatgcgcg tcaacgaagc attgttcgaa
      121 cgcacgagtt gttcgagtcg ctcgatcgag agcgcgagat gcgagccacg cttgtcaccg
      181 acaacagcat ggatttcgtc gacgatcagc gtgcgcgcgg tttggagcat ccggcggccg
      241 ccttcgctgg tcaaaagaat gtagagcgac tcgggcgtgg tgacgacgat gtgcggcgga
      301 cgctttgtca tcgctgttcg ttccgaggcg ggggtgtcgc cggtgcgaac gagcgtgcga
      361 atgttgactt cgggcagacc gagcgctttc agctcttcct gaattccttc gagtggcacc
      421 tggagattgc gctgtacgtc gttgctgagc gctttgagtg gtgagacgta gatgacgtgc
      481 gtggagtcgt cgagatttcc ttcgacaccg aggcggacca gatcatcgat cgctgacaaa
      541 aacgcggcga gagtctttcc cgatccggtg ggcgcggcaa tcagcgtgtg ccgctgcttc
      601 ttgatctcag gccatgcttg taactgcggg tccgttgggg cgggaaagct gcgcgcaaac
      661 cagcgcgcca ctgctggatg gaagatttct agattcataa gggacggaca ttatacttcc
      721 gtcacccgtg acttgcatag ctgaatcacg agcccaacca tgaaacaagc aatccgcgtt
      781 ttcaatccgc gtaaatccgc gtttactcaa actgttttct gaattgctca aactgctctt
      841 tgagcttctc gacttccgcc gcaagctcgt caaccttctg ttccagaccc gctcgccgtg
      901 acggactcgc cgcagccacg cgctcggttt cagccagcgc ttcgagatcg atctcgccat
      961 taagcaagtg cgcgaaccgg ccttccttct gtcccggctg acgcggcagt cgcgcgacga
     1021 gtggctccga gtcgtgcgaa atcagcccgt tgagcgtcgt ctcaatttct tccagactcg
     1081 caaagtcgtg caaacgtccc gcattgccac gcaactcacc gagcgtctgc gcaccgcgca
     1141 acatcaaagc acacatcagc gccacttccg gatgagtcag gtgcatcact tccggcatca
     1201 cgtgcttgta cttcggcacg cgactcgtgc tgccgtaaaa aacgtacgcg aggtttttct
     1261 cacgcaggct ctccaccgcg tcagccacct ggttctcggg ataagttgtg acggggttgc
     1321 gattgttctt ctggttgcaa gcgagcgtca gcgcgttgag cgtgagcgga tagtattcgg
     1381 gtgtcgtgac ctgcttctcg attagcgagc cgagaacgcg ggtttcaatg tcggttagga
     1441 atgtcgtcat tgcagtctca aaaaatatag gtcctatagg actaatagga cctatatgtt
     1501 ttcaaattaa actcgctttt attgatcgtc gccgtcagca gtgctcgggc gttgcccgcg
     1561 cggttcggag cgtccgacgt tcaggttgtc gtaattcaac gccgcattga acaccaggaa
     1621 aaactcacct tgcgtttggt gccgccacat cgggttcgtc gcaaacataa caacatgccc
     1681 gcgcccgacc ggcacatcaa caattgccgg acggttcgcg agctcattgc cgcccgcaag
     1741 catgccgctt atgagcaggt tcttttcatc tgacgcaaaa cgcagcacga cgcgtggtcg
     1801 cagctgcggt ggcacgaaga acgggctctc acgcaggtcg gcatccggat ccggccggtt
     1861 gtttggatct gcaggtcgcg gttgcggcat cgcctgaatc acgtccggat cgttaagacc
     1921 gccgcgtccg gaagcgcgcg aggcagcgcc accaccgcca ccgcccggac cgcctcctgt
     1981 ctggacctga aacagcggcg actggctgaa gtaaatgggc aaaccagcat cgtaaccata
     2041 agtaatcggg ctcttccgat ctgcgaacgt cgagttgtaa atcgagccgc gcgcctgaag
     2101 cttatcagcc ggttgaatcg aaacgccggt cgtgatgccg tagtcgatcg gcacttgcga
     2161 gacgccacca atcgtgatga acagacctcc gtcttcgatg aacttctgca agttggagac
     2221 gccggcgacc gtcattccac cgcgcatgtc gtcggtttga tccggcgagt taccaaagtt
     2281 gggcgtaacc gccgacgcct tccacggaat cgggtcgcca cgtttcggca tgccattaac
     2341 gatcgtctgc gctgagccac cgacaggcgg gaaaatcagc acgtcaaact tctcgcgcag
     2401 gttcggtgtg ttgccgatca cttgatcgga gatgtacgtg taaggaatct ggtacttatc
     2461 aaactcgatc cgataccagc cttcgttttg cgtgttgatc catgtgtgaa caatcgcgat
     2521 gcgcgggacc gcgagctcgt gcgtctttac ttccggcagc ttatcgacac caactgcctt
     2581 cagcccgagc tcagtagcag cagactccag ccgcgctctt agatccgcgg gattgccgtc
     2641 tttcttgatg atgaacgagc cggtgttgta ttgctgatcg ccaaccttga acgagtcttc
     2701 cgcggcgctc atcttcacat ctttcaaacg gaagcgcagc gtcgcgagcg tgttgtcggt
     2761 gttgtgattg atcaggtaag cgaccgcaga ggctgggccg gctagttgtc cggttacttt
     2821 tgcgtcctgt gtgagcaagg tcgcaggcgc gtcgagcacg ctcttgtcga cgacgcgcac
     2881 ggttttgacg ttgcgcatcg cacccatggt ccagccggtg tcgtcatacg gcgccgggtc
     2941 gctgacgttg taatactgcg tgtcgagcag catgtc
//
LOCUS       DASSFQ010000096         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_340305, whole genome shotgun sequence.
ACCESSION   DASSFQ010000096 DASSFQ010000000
VERSION     DASSFQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340305"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFH96_03075"
     CDS             <1..505
                     /locus_tag="VFH96_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6972976.1"
                     /translation="TLPVARMGQKSVPQVEQVEIVAPAVTQAAPPVPATERPRTRMLV
                     VDDEDFVRELLSEILEGEHCDVYVAANGSEALSMFRDMEFDAVFTDVGMPGMSGWEFA
                     REIKQINGNIPIAVITGWGEAVGSHEQKAAGVDWVIAKPFTADRIAELVRDIDLARQK
                     SVATAAA"
     gene            complement(583..948)
                     /locus_tag="VFH96_03080"
     CDS             complement(583..948)
                     /locus_tag="VFH96_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6972977.1"
                     /translation="MADILVVDDDDVIRDTLCELLSTDYSCQTAHTAEEALAKLEAQG
                     FDVVLTDISMPGLNGKELLKKVVELYPGTTVIVVSGHSDQIEAETLLSHGAFDFLLKP
                     FRLEMVEESVKRALISKVH"
     gene            1097..1540
                     /locus_tag="VFH96_03085"
     CDS             1097..1540
                     /locus_tag="VFH96_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6972978.1"
                     /translation="MTTPGVKATVKFVSDDLFLGVTPSGHALPLDTDGQRSTAPSPVE
                     LLLVALGTCTATDVAGILAKKRLDVTSYVVEVTGTRRDDFPRKYTAMKVHHILTGKSL
                     SPTAVAHAIELSDTKYCSVAATLRPTVEIQTSFEIIEAPRDEPRE"
     gene            1547..2956
                     /locus_tag="VFH96_03090"
     CDS             1547..2956
                     /locus_tag="VFH96_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6972979.1"
                     /translation="MLKIFVAVFLFLLCALPTAANQSASETTLAAVRRSDAADRDAQG
                     NLRKLTPAEHMRRAAIYLSVRAFAEARAHWQTLIDNYPQDPRVPEALLGIGRSYFLGK
                     VYQDGYVVFNQLAQQYNGTKEGREALNFSAASLLRMGKFSEAAARYAEYVDRFPGGER
                     IDTAHLNVIDTLREANKPAEALQWVTRTRQRFAGTAVETNAMFAALRLYVAEEQWTDA
                     VNMAEQLLKRSFPKAVQTSPSEVAYLKAYSLEHGGRKNEAFTAYLTVPDSIESYYGWL
                     ATQRLEALAGPSRAEIVAQREERVNTAIDSVADTYPAPYRQAILSAAKSRKLDPRFIL
                     ALIRQESVFKPLAKSPAGARGLLQLTMDAAQKYGPGAGLNTLRENQLYQPETSITVGA
                     EYIEHLTSLFPHMLEPVAASYNGGEDNVARWLERSKRTDPGVFTAEVGFDETKDYVQK
                     VMSNYRVYVRLYTVDLSRR"
     gene            complement(3001..>3323)
                     /locus_tag="VFH96_03095"
     CDS             complement(3001..>3323)
                     /locus_tag="VFH96_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972980.1"
                     /translation="AKLGQTANLGVIAAVFFRERSRPVPITPEYPITRDKDRMEAPQQ
                     SAQSVRRSDDEKAATGIGRNVRNDVRWVDMDLDSRAAGEMTIRYEYRRWREDSRGFCP
                     EPKY"
BASE COUNT          752 a          944 c          909 g          718 t
ORIGIN      
        1 caccttgccg gtcgcaagga tgggccagaa gagcgtgccg caggttgaac aggtcgagat
       61 tgttgcgccg gcagtgacac aggctgcgcc tccggtaccg gcgactgaac gaccgcgtac
      121 gagaatgctc gtggtggatg atgaagattt tgtgcgcgag ttactgagcg agattctcga
      181 aggtgagcac tgcgatgttt atgtcgcggc gaatggcagc gaggcgttgt cgatgtttag
      241 agatatggaa tttgatgctg tgttcacaga tgtcggcatg ccgggcatga gtggctggga
      301 gttcgcgcgt gagatcaagc agatcaatgg gaacatcccg atcgccgtga tcacgggctg
      361 gggcgaagcg gtcggatcgc atgagcagaa agctgcgggt gtcgattggg tcatcgcgaa
      421 gccgtttacg gctgatcgaa ttgccgagct cgtgcgcgat attgatttgg cgcgccaaaa
      481 atccgttgcg accgcagcag cctgatagca gttagacaag attgcaggat ttgcaaaatt
      541 aacaagacaa ttctgttaat tctgagaatc ctgtaatcct gtctaatgta cttttgaaat
      601 cagtgcgcgc ttaacgctct cctccaccat ctcgagccgg aaaggtttca acaaaaaatc
      661 aaacgcacca tgactcagca gggtctccgc ctctatttga tcgctgtgtc cggaaacgac
      721 gatcaccgtt gtgcccggat acaactcaac caccttcttc aacagctcct tgccattcaa
      781 acccggcata gaaatgtccg tcagcacaac atcgaaacct tgcgcttcca acttcgctaa
      841 cgcttcttct gcggtgtgtg ctgtttgaca ggaatagtcc gtcgataaca actcgcagag
      901 cgtatcgcgg atcacgtcgt catcatcgac gaccaggatg tcagccatag ttctcctcca
      961 acaccgtaac ttaggcatct cctgggcgac gatatcgatt ttccttcgaa tctaattcgt
     1021 gatgatgagg tccaactcca acgtctgaat cctaacatag tatcgcgtcg aacagcgtat
     1081 gttattcttt tgcgcgatga ctacgcctgg cgtgaaagca accgtgaagt tcgtgagcga
     1141 cgatttgttt ctcggcgtca cgccgtcagg tcacgcacta ccactcgaca ccgacggcca
     1201 gcgatcgaca gcgccctcgc cagtagagtt gctgctcgtc gcactgggta cgtgtactgc
     1261 aaccgacgtc gcgggcatcc tcgcgaagaa gcgcctggac gtgacgagtt atgtggtcga
     1321 ggtgacagga acgcgccgcg atgactttcc acggaagtac acggcgatga aagttcatca
     1381 catcctcacc ggcaaatcgc tttcgccgac agccgtcgcc catgcaatcg aactctccga
     1441 cacgaagtac tgtagcgttg cggcaacgct gcgacccaca gtagaaatcc aaaccagctt
     1501 cgagatcatc gaagccccca gagatgaacc tcgggagtaa ttgagggtgt taaaaatctt
     1561 cgtcgctgta tttctttttc ttttatgcgc gcttcccact gctgccaatc agtctgcgag
     1621 tgagacgact ctcgcggccg tgcgacgcag tgacgccgcc gatcgcgacg cgcagggaaa
     1681 tctccgcaag cttacacctg ccgagcacat gcgccgtgcc gcgatttatt tgagcgtgcg
     1741 tgcgtttgct gaagcgcggg ctcactggca aacactcatc gacaactacc cgcaggatcc
     1801 acgcgtgcct gaagcgctgc tgggcattgg tcgttcttat tttctcggca aggtctacca
     1861 ggacggttat gtcgtgttca atcaactggc ccagcaatac aacggcacga aggaaggacg
     1921 tgaagcgttg aacttcagcg ccgcttccct tctccgcatg ggcaagtttt ccgaagcagc
     1981 cgcgcgctac gctgaatacg tcgatcgctt tccgggtggc gagcgcatcg acaccgcgca
     2041 tttgaacgtg atcgacacgc ttcgtgaagc aaacaaacct gccgaagcac tccagtgggt
     2101 gacgcgcacg agacaacggt tcgcaggcac ggcagtcgag acgaatgcga tgttcgccgc
     2161 gctgcgtctc tacgttgcgg aagaacaatg gaccgacgcg gtgaacatgg cggaacagtt
     2221 gttgaagcga tcgtttccga aagcagtgca gacctcgcct tctgaagtgg catatctcaa
     2281 ggcgtacagt ctcgaacacg gcggacggaa aaacgaagcg ttcacggctt atttgacggt
     2341 gccggacagt atcgagtcgt actacggctg gctcgcaacc cagcgtctgg aagcactcgc
     2401 tggaccatcg cgcgcggaaa tcgtcgcaca gcgtgaggaa cgtgtgaaca ctgcgatcga
     2461 ttcggtggct gatacttatc ccgcgccgta tcgacaggcg atattgagcg ccgcgaaatc
     2521 tcgtaaactc gatccgcgtt ttattctcgc gctgattcgc caggagagtg tgttcaagcc
     2581 actcgcgaaa tcaccggcgg gcgcgcgcgg gttgttgcag ttgacgatgg atgcggccca
     2641 gaagtatgga ccaggcgcgg gcttgaatac gttgcgcgag aaccagctat accagcctga
     2701 gacttcgatc acagtcggcg ccgagtatat tgagcacctg accagtctgt ttccccacat
     2761 gctcgagccc gttgccgcgt cgtacaacgg cggtgaagac aacgtagccc gctggctgga
     2821 gcgctcgaag cgaaccgacc ccggcgtgtt cacggccgaa gtcgggttcg atgaaacaaa
     2881 agattacgtc caaaaggtga tgtcaaatta ccgcgtctac gttcgactct atacggttga
     2941 tcttagccgc agatgaacgc ggatgaacgc tgatctgcgt ttatccgcgt gcatccgcgg
     3001 ctaatatttc ggctcgggac aaaaccctct cgagtcttca cgccaacgcc gatactcata
     3061 cctgattgtc atttcccccg cggcacgtga atcaagatcc atatcaaccc aacgaacatc
     3121 atttcgcaca ttcctcccaa tccccgtcgc cgccttttca tcatcactcc ggcgtacgga
     3181 ctgcgccgac tgctgcggcg cttccatccg atccttgtca cgcgtaatcg ggtattccgg
     3241 cgtgatcggc actgggcgac ttcgctcacg gaagaacaca gcagcgatca ccccaaggtt
     3301 cgcagtctga ccgagcttcg ccc
//
LOCUS       DASSFQ010000097         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_489450, whole genome shotgun sequence.
ACCESSION   DASSFQ010000097 DASSFQ010000000
VERSION     DASSFQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489450"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFH96_03100"
     CDS             complement(<1..530)
                     /locus_tag="VFH96_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972981.1"
                     /translation="MRNIKQLTFGGENAEAYFSSDGKQLIFQSTREGHGCDQIYTMNV
                     DGSNVKMISNGDGRTTCSYFFPGQKQVLYSSTYLGDKQCPPRPDYSKGYVWAVYPAFD
                     IFTANPDGSNLRQLTNSPGYDAETTITLDGKKLVFTSMRDGDLDIYTMDANGKNVRRL
                     TNELGYDGGPFWSYDG"
     gene            complement(632..1468)
                     /gene="lgt"
                     /locus_tag="VFH96_03105"
     CDS             complement(632..1468)
                     /gene="lgt"
                     /locus_tag="VFH96_03105"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET6972982.1"
                     /translation="MYPEIFHIGSFPINTYGVFLALAFLCAILITVRLAKRDGLPHEK
                     IYDLSLWMLLAALVGSKILMLFTEPEYRENPAQLLSLDFLRSGGVFYGGLLGAVVAGY
                     FLMKRYKLPWWKTADACAPGIAIGNFFGRQGCFAAGCCWGKPTTLPWGVKFTELGHQI
                     TGVPTDTYLHPTQLYESFAMLLVFLFLLWLHKHKRFSGQVILAYAVLYSAIRFSIEFV
                     RDDPRGDVFGLTSLTGLSTSQMISLVIGITALILLIVRRRRSSHPVNLENNPVILSKT
                     AV"
     gene            complement(1458..1997)
                     /gene="lspA"
                     /locus_tag="VFH96_03110"
     CDS             complement(1458..1997)
                     /gene="lspA"
                     /locus_tag="VFH96_03110"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET6972983.1"
                     /translation="METSIEKATDIRARFAWRAGYLLAAFGIYMSDQASKAWAVRTLR
                     FGDERVVIPGFFQFIYTENPGIAFGQLQEGGSFGRWFFVVLAVLAALAVLFYFMRTPR
                     NDDRVLGACALLLAGISGNLTDRARLGYVIDFIVLHAGDYHWPTFNIADASITVGALL
                     LAYDLIFSHRSSRIKTDVS"
     gene            complement(2009..>2732)
                     /locus_tag="VFH96_03115"
     CDS             complement(2009..>2732)
                     /locus_tag="VFH96_03115"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6972984.1"
                     /translation="DRWALAELNRVVVEVLAAYEAFEFHNVYRALFNFATVTLSARYF
                     DIIKDRLYTFAPRNKARRSAQTALLQIGDALARMLAPLLVFTADEIWENLPGRDEPSI
                     HLALLPKADEQSHDELLTEWERLFAIRDNVLRALEEARIAKQIGSSLEAKVTLRAAGS
                     ALELLQRHQNDLRYLFIVSQVELGNSDASEGVSITISNADGQKCERCWNYSTRVGEWD
                     RYPTVCERCVAALKEIEEGAAD"
BASE COUNT          684 a          755 c          738 g          555 t
ORIGIN      
        1 ttgccgtcgt acgaccagaa tggaccgcca tcgtaaccga gctcattagt cagccgccgc
       61 acgttcttgc cgttagcatc catcgtgtag atatcgagat cgccgtcgcg catcgaagtg
      121 aagacgagtt tcttgccgtc gagagtgatc gttgtctcag cgtcgtatcc gggcgagttg
      181 gtaagttgcc tcagattgga accgtcaggg ttcgccgtga agatgtcgaa agccggatag
      241 accgcccaca cgtagccttt cgagtagtct ggccgaggtg gacactgctt atcgccgaga
      301 taggttgacg agtaaagtac ctgcttctga cccgggaaga agtacgaaca agttgtgcgg
      361 ccgtcgccgt tcgagatcat cttcacattc gatccgtcga cgttcatcgt gtagatctga
      421 tcgcagccat gtccttcgcg tgtcgattga aagatgagct gtttaccgtc agacgagaaa
      481 tacgcctcgg cgttctcgcc gccgaaagtc agttgtttga tattgcgcaa gtgcttttcg
      541 cctgggagaa cgagttgatt atcttgtgac gtggccggag agatgcagaa gagtactgcg
      601 acgaccaaaa gagggattag tcttagtgag ctcatacggc tgttttagac aggattacag
      661 gattattttc aagatttaca ggatgagagc ttcgacggcg acggacgatc aacagtatca
      721 atgcggtgat gccgatgacg agactgatca tctgcgaagt tgaaagaccg gtcagactcg
      781 tcaaaccgaa aacatcgcca cgcggatcgt cacgcacgaa ttctatagag aagcgaatcg
      841 ctgagtacag cacagcgtaa gcaagaatga cctggccgct gaaacgtttg tgtttgtgca
      901 gccagagtaa aaacaaaaag accagtagca ttgcgaacga ttcgtagagc tgcgttggat
      961 gaaggtaagt atcagtcggc acacccgtaa tctggtggcc aagctccgta aacttcacac
     1021 cccacggcag tgtggttggt ttaccccaac agcaaccggc cgcaaaacat ccctgccgtc
     1081 cgaagaaatt gccgatcgcg attcccggcg cacaggcatc ggccgtcttc caccacggca
     1141 gtttgtaccg cttcattaag aagtagccgg cgacaacagc accgagcagt ccgccgtaga
     1201 agacgccgcc ggaacgcaga aaatcgagtg acaaaagctg cgcgggattc tcgcggtatt
     1261 cgggttcggt gaacagcatg aggatcttcg aaccaacaag cgcagccagc agcatccaca
     1321 aactgaggtc gtaaatcttt tcatgcggca aaccgtcgcg cttcgccagt ctgactgtga
     1381 tcaaaatcgc acagagaaac gcgagcgcga gaaagacgcc gtaagtatta atggggaaac
     1441 tgccgatatg aaaaatctca ggatacatct gtctttatcc gcgacgatct atggctaaaa
     1501 atcaaatcgt aagcaagcaa tagcgctccc accgtgatgc tcgcgtcggc gatattgaat
     1561 gtgggccaat gataatcgcc ggcgtgcaaa acgataaagt cgatcacata tccgaggcgg
     1621 gcgcggtcag taaggttgcc ggaaatgccg gccagcaaga gcgcgcacgc gccgagcacg
     1681 cgatcgtcgt tacgcggcgt gcgcatgaag tagaaaagga ccgccagggc ggcgagcacg
     1741 gccagcacga cgaagaacca gcgcccaaac gaaccgcctt cctgtaactg gccaaacgcg
     1801 atgccgggat tctcggtgta aataaactga aagaagcccg gaatcacgac gcgttcgtca
     1861 ccgaaacgaa gtgttctgac cgcccacgcc ttcgacgcct ggtcggacat gtagataccg
     1921 aacgcggcca gcaaataacc ggcgcgccac gcgaaacgcg cgcgaatatc agttgccttt
     1981 tcgatgctgg tttccaagag tgtccgcgtt aatccgcggc cccttcttcg atctccttca
     2041 atgcggcgac gcagcgctcg caaacagttg gataccgatc ccactcacca acacgcgtgg
     2101 aatagttcca acagcgttcg cacttctggc cgtcagcgtt ggagatcgtt atcgaaacgc
     2161 cttcgctagc atccgagttc ccaagctcta cctgcgaaac aataaacagg tagcgcaagt
     2221 cgttttgatg ccgttgcaat aactccaacg cacttcccgc cgcccgtaga gtcaccttcg
     2281 cttccagcga gctaccgatc tgcttcgcaa ttctcgcctc ttcgagcgct cgcagcacgt
     2341 tgtcgcgaat cgcaaacaat cgctcccact ccgttaataa ctcgtcgtga ctttgctcat
     2401 cagctttcgg caacaaagcg agatggatcg acggctcgtc acggcccggg aggttttccc
     2461 agatctcgtc ggcagtaaaa acgagtagcg gcgccagcat gcgcgcgagt gcgtctccga
     2521 tctgaagcaa cgccgtttgc gccgagcgac gcgccttgtt gcgcggcgca aaggtgtaaa
     2581 gccgatcctt gatgatgtcg aaataacgcg ctgacaacgt caccgtcgcg aagttgaaca
     2641 acgctcgata gacgttgtga aactcgaaag cttcgtacgc ggccaacact tctacgacga
     2701 ctcggttgag ctccgcgagt gcccagcgat cg
//
LOCUS       DASSFQ010000098        16125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_38608, whole genome shotgun sequence.
ACCESSION   DASSFQ010000098 DASSFQ010000000
VERSION     DASSFQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16125
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38608"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1056)
                     /locus_tag="VFH96_03120"
     CDS             complement(94..1056)
                     /locus_tag="VFH96_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972985.1"
                     /translation="MSERVPEHLVIWEFIAAGACFAVAVIAIALGFVFTTQWLLDDHV
                     HPVLHTLGLVLLIIGIPIMMLGAHFMDVQEKKLDESRKNSKSVHVVGLVSVIGVLFIP
                     SSVVHAQQTIFNVPSTDVLDKGKVYAELDASLKPTDGSDVPKFSSFVPRVVIGAGSNI
                     EFGLNVTGNIQPGPDSTTLVPTIKWRPYKGENGWTIVLGNNLFVPVRNRAYNAASYFY
                     AETSKTFKNGTRITFGGYDFTKNAVASANRAGGQFGFEQPLNKKITFAADWFTGKHAS
                     GYFTPGIIFKAGPKFTGYAGYSIGNQNVSGGNHFFLLELGYNFN"
     gene            complement(1193..1909)
                     /locus_tag="VFH96_03125"
     CDS             complement(1193..1909)
                     /locus_tag="VFH96_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6972986.1"
                     /translation="MTNQARILVVDDEPQLTRVLRTGLKSRGYDVRAAADGLAGFEAF
                     NDWHPDLVITDLAMPNVDGLELCRRLRAVSTVPIIVLSAKGEEKTKVEALDLGADDFV
                     TKPFGIDELLARVRASLRRAKAPATNEATQTMLDSGDFHVDLETREITVRGKSIHLTP
                     KEFDLLVYFIKHSGKVLTHRTLLAALWGGNYVEQNEYLRVFVGNLRKKIEPDAASPRY
                     ILTEPWIGYRFDPDQVISHR"
     gene            complement(1906..3111)
                     /locus_tag="VFH96_03130"
     CDS             complement(1906..3111)
                     /locus_tag="VFH96_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6972987.1"
                     /translation="MQRSVLDQKWAGYVAAALGVGLLTLLLKTVGAHVNAATVSLALL
                     LTVLFIATRWGSLPALAASILAMLCFNFFFLPPLGTFTIAATDNWIALLAFLITAVTA
                     GQLSGNAKRRAEEAETGRREIERLYAELRDAFGRASHAEALRQSEKLKSALLDAVTHD
                     LRTPLTSIKASITTLLDEVRDNEPPVKLDAESRVEMMEVIDEESDRLSRFINGLIELA
                     RIEAGELQLRRRWGAVEEIISTALSRAEPITHSHRVEVDVEKELPGVRVDERAVSEVV
                     YTLIDNAAKYSTVGSTICITARRSGDDLIEMGVEDDGPGIPADLRERVFDKFFRATRD
                     GDVRSRQPTGTGMGLAIAKGIVEAHYGKIWIEPGRDGKGTRVLFTLPIGDDDVIEEPR
                     YTTATVDAQ"
     gene            complement(3212..4045)
                     /locus_tag="VFH96_03135"
     CDS             complement(3212..4045)
                     /locus_tag="VFH96_03135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6972988.1"
                     /translation="MTENMLDAWRESAKYWTEHSETIHQMFVPLTRALIERAGIHQGQ
                     KVLDVAGGAGEPSLTIAETVGPEGSVTFTDGVAQMVETARARAQQRGLTNIQFHQSVA
                     ESLLFADESFDVVVSRLGVMLFADPDLAMREMLRVLKPGGRLAFAVWGKSDINPFCYL
                     VTRVMDQHVKSPAADPDAPSAFRFAEPGKLTSVMTQAGAIDVAEDVVTFDLEAPISPR
                     QFWTMRSQMSDTLRDKLTTLSADEQAQIAVEVEQAVKEFFPAGRMKFPAQFIIATGNK
                     R"
     gene            4069..4773
                     /gene="pepE"
                     /locus_tag="VFH96_03140"
     CDS             4069..4773
                     /gene="pepE"
                     /locus_tag="VFH96_03140"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HET6972989.1"
                     /translation="MNDKRLLLISNSTLHGSGYLDHAESEIRSFLGDAKRVLFVPYAL
                     FDRDKYAANARQRFQKMEYELTSVHTAADPVAAVNETDAVFIGGGNTFRLLKALYEFD
                     LIEAIRKRVSKGMPYIGSSAGSNVAAPTIKTTNDMPIVQPPSFNALGLVSFQINPHYL
                     DPDPNSKHMGETREERIVQFLEENDTPVVALREGALLRIENGETILRGSTGARIFRKG
                     FETVERAPGARLDDLF"
     gene            complement(4792..6075)
                     /locus_tag="VFH96_03145"
     CDS             complement(4792..6075)
                     /locus_tag="VFH96_03145"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET6972990.1"
                     /translation="MTDVDLARLQRDTFKYFADEVNPENGLVRDSTRRGSPSSIAAVG
                     FALTSYPIAVERKYLTRAEAVQRTLATLRFFHDAPQGKQRDATGYKGFYYHFLDMETG
                     RRANKCELSTIDSTYLLAGALAASCYFDADTPAEREVRELAEALYKRADWHWAQHGAL
                     TVSHGWRPERGFIRYRWSGYSEALILYVLGLASATFPLPAESYQAWTRTYKWKKLYEQ
                     EFVYAGPLFIHHLSHMWIDFRGIQDDYMRGKASDYFENTRRAIYAQQTYAIRNPKKFA
                     GYGRFSWGITASDGPGPAEQRVNGRRVRFFDYKARSIPYGPDDGTLAPWAVAASLPFA
                     PEIVLPSLFRLNKDYPEITNEYGFKCSYNPTFSSGGQKGWISQGYYGLDQGPIIMMIE
                     NYRTGFIWRLMQRCPFIVSGLRRAGFRGGWLENPP"
     gene            complement(6099..6668)
                     /locus_tag="VFH96_03150"
     CDS             complement(6099..6668)
                     /locus_tag="VFH96_03150"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HET6972991.1"
                     /translation="MKIEVNMRDNDSNLLKGMVAGLAGGLLASWTMNQFQAAWTRVAE
                     GTEKSHGAQSMQPAEGSQGEQAQDTAEQDDATVETAKAISKNVLGHELQESEKESAGA
                     AVHYAFGTLSGGLYGALAEVTPQVTTAGGLPFGAGFWLLADEISVPLLGLSKGPTEYP
                     VSTHVYALASHLVYGVTAELGRRAVRQVL"
     gene            complement(6834..7259)
                     /locus_tag="VFH96_03155"
     CDS             complement(6834..7259)
                     /locus_tag="VFH96_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6972992.1"
                     /translation="MSTKAGTSPGRKKASNMFKEAKTFSSFSVNDLQKAKQFYSQTLG
                     LDVKESPEGLELHLDDNAVFLYPKPNHTPASFTVLNFAVDDIDAAVDELTGLGVKLEH
                     YDLPDIKTDERGIARGPHGPMIAWFKDPAGNILSVLEEK"
     gene            7423..8334
                     /locus_tag="VFH96_03160"
     CDS             7423..8334
                     /locus_tag="VFH96_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6972993.1"
                     /translation="MLNQYTYQATLAASESIHWRVEDIIGGEKRLNFTQPFMPEALAN
                     TEPLTFLSREEKRILNQIRGNAYLCIFGLVEEFILPFVLDHARPQLRGDDYRVRALLR
                     FASEEAKHIQLFKRFRTEFEDGFGTLCNVIGPPDAIAAAILVHDPLAIAITTLHIEWM
                     VQSHYLDSIKDDQELDAQFKSLLKHHWVEEVQHAKLDTLMVEALAADRTEEQIMSAVE
                     EYLEIGGFLDGGLQQQVEFDLTSFEVASGRRLTDAEANEFRRVQVRANRWTYLGSGMT
                     HAKVIETLESLTPKARQRVESVSLAFC"
     gene            complement(8388..9017)
                     /locus_tag="VFH96_03165"
     CDS             complement(8388..9017)
                     /locus_tag="VFH96_03165"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6972994.1"
                     /translation="MRRCIAITILLGLIVFTACNDPAADTTKARTGEAARVASPAAAP
                     GQKYQITSQNSKIEFIGSKVTGKHNGSFGDFSGQVDYTGNPETSRVNITIKTDSITTD
                     TPDLTKHLKTADFFDVAKFPEASFVSTAIKAGGEKGATHTITGNLTMHGVTKAVTFPA
                     TINVTPDAATVESSFSINRKDFGINYAGAADNLIRDDVVMTLHIRATKS"
     gene            complement(9048..9896)
                     /locus_tag="VFH96_03170"
     CDS             complement(9048..9896)
                     /locus_tag="VFH96_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET6972995.1"
                     /translation="MRAQSLVVALLMMSFAAYGQTGRSMSTDQRVLTGIDWRLVSLGP
                     AGAETNVVAGTTVTLRFGEDNRASGSTGCNSFSGTYQVRGDNISFSRLISTRRACLDQ
                     NANEQEQRFLSTLGAANRFRLTRGRLTILSDRGRSVLNFVNNSISEPQDGPREDRSDP
                     VATLAAYYSAINARDYRSAFRFWDSPPSSYEQFSRGFNDTDQVRLLVDPSTRVDGAAG
                     SVYAEIPAIVVSTMRNGSERVFAGCYTMRRSNVQDRGWQIYRADVSPVSANIRLSRIL
                     SQGCRN"
     gene            9972..11105
                     /locus_tag="VFH96_03175"
     CDS             9972..11105
                     /locus_tag="VFH96_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6972996.1"
                     /translation="MFYDVAVIGAGVFGAWSAYQLRLTGASVLLLDAYGPGNSRASSG
                     GESRIIRLGYGPDEIYSRSSQRSFTLWQQLFEKTEPLFHKTGVLWLAREHDAYCEATL
                     KTLQRLNANFERLDQDELIRRFPQLELGATKWAILESDSGVLMARRAVQAVVAQAQAN
                     GVEYVEKAIAPPQGRLDSIRTVSGEAIEAGQFVFACGPWLPKLFPELLKDLIYVTRQE
                     VIFLGVPPGDDSFNRGSMPAWIDFNDLVYGLPNVDGRGFKIAIDAHGAEFDPDRGERV
                     ISVDGLKAMREYVAKRVPRLAHAPVLETRVCQYENTSNGDFLIDRHPAFENVWIVGGG
                     SGHGFKHGPVVGEYVARMIAGEQVREPRFGLESKQRVQNRAVY"
     gene            complement(11192..11923)
                     /locus_tag="VFH96_03180"
     CDS             complement(11192..11923)
                     /locus_tag="VFH96_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET6972997.1"
                     /translation="MKSKLCSLTCMAAILLLAPVCVADSNKGQNRLLKQQERYTLSLE
                     LEFTKKEQNPLEQAISVLFDVGPNGYATGFLVGNGLVMTAYHVISGNLSTTKKVMLGF
                     KGTDELSVKVFVDGCRAKVIKVDKEADLALLEMCRNSKSAKAPKFQTAPNRDDKLFLI
                     ARPHGDKVVSHGSFYGNYMLGNQEYWSVKIDSRDGFSGSPVYNSEAEVVGVFSGYDWS
                     QKLALISPSIRAQKLLEDYNATAKP"
     gene            complement(12172..13503)
                     /locus_tag="VFH96_03185"
     CDS             complement(12172..13503)
                     /locus_tag="VFH96_03185"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET6972998.1"
                     /translation="MKLLIANGYVIDPAQNLNTGGRGVLIEDGRIAGLLERGEPVPEQ
                     AHVIDATGLIVAPGFIDMHTHLREPGQEYKETVATGASAAVAGGWTSICAMPNTDPVN
                     DNPAVTRFIIDQGRAAQLANVFPIGAVTKSSQGKELAEMGEMKNAGIVAVSDDGRSVP
                     TSSMMRRALEYARGFDLTLVDHCQDQSLSAGGVMHEGRWSLILGLRGMPAAAEDVDVA
                     RDCVLAKLTKAKVHIAHVSTRGALEAVRRAKHEGLNVSCEVTPHHWTLTDEAVSEYDT
                     NTKMSPPLRSREHVDAVLEAMKDGTIDAIATDHAPHHLDEKGLEFDQAPFGITGLETA
                     VGLAFDLVHAGLIDLERVVQMCSTNPARIFGLTDRGSLKKDAHADVTLLDPNLEWVFD
                     VSRSKSKSRNTPFHNRAMHGAAVATIVGGRLVYLHPDYTRINQPRQKTASV"
     gene            complement(13540..14511)
                     /locus_tag="VFH96_03190"
     CDS             complement(13540..14511)
                     /locus_tag="VFH96_03190"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET6972999.1"
                     /translation="MEATFNRRHLLGIRELEAGEIVHLLDTAESFREVSKREIKKVPA
                     LRGRTVINLFFESSTRTRTSFEIAAKRLSADAINISVSTSSVTKGETLLDTARNLEAM
                     APDCIVIRHSSAGAPQHLARMTNAPIINAGDGSHEHPTQALLDALTIREHKERIEGLK
                     VAIVGDILHSRVARSNIYLLTKLGAHVSVGGPGTLVPDEFAELVESGLHIEKHFEDAI
                     DGADVVMILRIQRERQDSAFFPSMREYAVHYGLQPRHLERAAPDAIVMHPGPMNRGVE
                     ISSEIADGSRSLILDQVTNGVAVRMAVLYLLAGGQEIKNHPQITQMS"
     gene            complement(14551..15135)
                     /gene="pyrR"
                     /locus_tag="VFH96_03195"
     CDS             complement(14551..15135)
                     /gene="pyrR"
                     /locus_tag="VFH96_03195"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET6973000.1"
                     /translation="MVVSPAHMDFVEKARIMDGPRINRALARLSSEIVEENHGAGDLF
                     LVGIRRRGVPLAERMAQKIADLEGYRPPVGVLDITLYRDDLSTVGAKPVVNRTDLPGD
                     IENRNIVLIDDVLYTGRTIRAALDQLIDFGRPRRVQLAVLIDRGKEHRELPIQADYIG
                     KLVPTKKSEIIKVMLQECDEEEGVVIVERPSETA"
     gene            15179..>16125
                     /locus_tag="VFH96_03200"
     CDS             15179..>16125
                     /locus_tag="VFH96_03200"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET6973001.1"
                     /translation="MSDKLKHVGHQTVPFVPFVANLYDVDPEMVYRSLVRPLLFRLPA
                     ETAHELALHSLSLLPASLITKRVDNPALSSKRFGLTFANPIGLAAGFDKNGIALQPLA
                     ALGFGFIEAGTVTYHAQPGNPRPRLFRLSEDQALINRAGFNNEGAAAFSRRVSNHRPD
                     CVLGVSIGKSKITPLENATEDYLASFELVHEVADYIAINVSSPNTPQLRELQQSQPLE
                     SLLSALQSRRRELQKHVPLLVKLSPDLDRRELETIVEVIERLNIDGIIATNTTVRREN
                     LRTNMKRVAACGEGGLSGRPLKDTSTRMIATLYELTGGR"
BASE COUNT         3589 a         4491 c         4379 g         3666 t
ORIGIN      
        1 gccacccgtc gtttgacatc gcgccgagac gggtggccac ggagggccac ccctacagtt
       61 aggtccaact agcattgttg atctccatgc acctcagttg aagttgtaac caagctcgag
      121 cagaaagaag tgattaccgc ccgacacgtt ttgattgccg atcgaatagc ccgcgtaccc
      181 ggtgaatttt ggaccagctt tgaagatgat tccgggcgtg aaatagccgc tggcgtgttt
      241 gccggtgaac caatcggcag caaacgtgat tttcttgttc agcggttgct cgaaaccaaa
      301 ctggccgccg gcacggttcg cagacgccac cgcattcttc gtaaagtcgt aaccgccgaa
      361 agttatcctc gtgccgttct taaacgtctt gcttgtttct gcgtagaagt agcttgcggc
      421 gttgtaggcg cgattgcgca ccggaacgaa gagattattg cccagaacga tagtccaacc
      481 gttctctcct ttatatggcc tccacttgat cgtgggaacg agcgtcgttg aatcaggacc
      541 aggttgaatg ttgccggtga cgttcagtcc aaactcgatg ttgcttcccg caccgataac
      601 gacgcgagga acaaacgaag aaaacttcgg aacatccgag ccgtccgtgg gcttcagact
      661 cgcatcgagc tccgcgtaaa ctttgccttt gtcgagtacg tccgtagagg gaacattgaa
      721 gatggtttgc tgcgcgtgaa caacagaaga aggtataaaa agaaccccta tgacactcac
      781 cagcccaaca acgtgaaccg acttactgtt cttgcgtgat tcatccagtt tcttctcctg
      841 cacatccatg aagtgggcgc cgagcatcat gatcggaatc ccgatgatga gaagaaccag
      901 ccctaaggtg tgcagaactg gatgaacgtg gtcgtccaac aaccactgcg tagtgaatac
      961 aaacccgagc gcaatcgcga tcacggccac agcaaagcac gcacccgccg cgatgaattc
     1021 ccaaataacc aaatgctccg gaactctttc actcatgcac ccaacttacg ccgccgcgcg
     1081 taaaaagggc gtaaagggtt tataaaaaga tcataaagaa ggggccacgg ataaacacgg
     1141 attacataca cggatctaaa cagataaaaa aatccgtgta tgaaatccgt gtttatctgt
     1201 ggctaataac ctgatcagga tcgaagcggt agccaatcca gggctcggtg aggatgtagc
     1261 ggggactcgc agcgtcgggc tcaatcttct tgcgcaagtt gccgacaaac acacgcaggt
     1321 actcattctg ttcgacgtag ttgccacccc aaagagcggc cagcaacgtc cgatgcgtca
     1381 acacttttcc ggaatgcttg atgaagtaga ccagcagatc aaactctttc ggcgtcagat
     1441 gaatcgactt gccacgcaca gtgatctcgc gtgtctcgag atcgacgtgg aaatcgccgg
     1501 agtcgagcat tgtctgcgtg gcctcattcg tggcgggagc tttggcacga cgcaatgaag
     1561 ccctgacgcg cgccagcaac tcatcgattc caaacggctt cgtcacaaag tcgtcagcgc
     1621 cgagatcgag cgcctccacc ttcgtcttct cttcaccctt cgctgacaac acgatgatcg
     1681 gcacggtgga aaccgcccgc aaacgccggc acaactccag cccatcgaca ttcggcatcg
     1741 ccagatccgt gatcacgaga tcgggatgcc agtcgttgaa agcctcgaag cctgccagcc
     1801 catcagcagc ggcgcgcacg tcgtaaccac gcgacttcag tcccgtgcgc aaaacgcggg
     1861 tgagctgcgg ctcgtcatca actacgagaa tgcgtgcctg gttcgtcatt gcgcgtccac
     1921 agtcgcagta gtgtatcgcg gttcctcgat cacgtcgtcg tcgccgattg gcaacgtaaa
     1981 caacacgcgc gtgcccttgc catcgcggcc tggctcgatc cagatctttc cgtagtgcgc
     2041 ttccacgatg cctttcgcga tcgcgaggcc catgcccgtg cccgtgggtt ggcgactgcg
     2101 aacgtcaccg tcgcgcgtcg cccggaagaa cttgtcaaaa acacgctcgc gcaagtcagc
     2161 cggaattccc ggcccgtcgt cttcaactcc catttcgatc aaatcatcac ccgagcgacg
     2221 tgcagtgatg cagatcgtcg agcccaccgt tgaatacttt gcggcgttgt caatgagtgt
     2281 ataaacaact tcgctgacgg cacgttcatc gacacgcacg cccggcaact ccttttcaac
     2341 atcgacctcc acacgatgac tatgcgtgat cggctcggcg cgggacaatg ccgtggaaat
     2401 gatctcttcg acggcgcccc aacgccggcg cagctgcagc tcgccggctt cgattctcgc
     2461 aagctcgatc agtccgttga tgaaacgact caaacgatcg ctctcttcgt cgatcacttc
     2521 catcatctcg actcgcgact ccgcatcgag cttgaccggc ggttcattat cacgaacctc
     2581 atcgagcagc gtggtgatcg aagctttgat cgacgtgagc ggtgtgcgca gatcgtgtgt
     2641 tacggcgtcg agcaacgcgg atttcagctt ctcactctgc cgcaaagcct ccgcatgact
     2701 ggcacgccca aacgcatcac gcagctccgc gtatagtcgc tcgatctcgc gtcttccggt
     2761 ttcggcttct tcggcgcgtc gtttggcatt gcctgagagt tggcccgcag tcacagcggt
     2821 gatcagaaag gcgagcagtg cgatccagtt gtcagttgcg gcgatcgtga aagtgcctaa
     2881 cggtgggaga aagaagaagt tgaaacagag catcgcgagg atcgatgcgg caagagccgg
     2941 caggctgccc cagcgagttg cgatgaacaa gacggtcagc agcaatgcga gcgagacggt
     3001 agccgcattg acatgcgcgc cgactgtctt cagcagcaac gtcaacaaac cgacacccaa
     3061 cgccgccgcc acataacccg cccacttctg gtccaacacc gaccgctgca tgacggctat
     3121 tgtatgacag tttttagcca cgaaaaggca caaaagtcac aaaacagaaa aacaactttt
     3181 gtgctttatg tgccttttgt ggctaaagag ctcatcgctt gttgccggtg gcgataatga
     3241 attgcgccgg aaatttcatc cggccggccg ggaagaactc ttttaccgcc tgctcgactt
     3301 caaccgcgat ctgcgcctgc tcgtccgcag atagcgttgt caatttgtct cgcaacgtat
     3361 cggacatttg cgaccgcatg gtccaaaact gtcgcggcga gatgggcgct tcgaggtcaa
     3421 acgtgacaac atcctctgcg acgtcgattg cgcccgcctg cgtcatcacg ctcgtgagct
     3481 tgcctggctc cgcgaaacga aacgcactcg gcgcgtcggg atcggcggcg ggtgatttca
     3541 cgtgctggtc catcacgcga gtcacgagat aacaaaacgg attaatgtcg ctttttcccc
     3601 agactgcaaa cgccagcctg ccgccgggct tgagcacgcg taacatctcg cgcatggcca
     3661 ggtcaggatc ggcaaataac atcacgccca gcctgctcac gactacgtca aatgactcgt
     3721 cggcaaacag caacgactcg gccacgctct ggtgaaactg aatgttggtg agaccacgct
     3781 gttgggccct ggcgcgagcc gtttcaacca tctgagccac gccgtcagta aacgttaccg
     3841 atccttctgg tccaaccgtc tcggcgatcg tgagcgacgg ttcacctgcg ccaccggcaa
     3901 cgtcgagcac cttctgccct tgatgaattc cggcacgctc gatcaaggca cgagtcagcg
     3961 gcacaaacat ctggtgaatg gtttcgctgt gttccgtcca gtacttcgcc gattcacgcc
     4021 acgcgtctaa catgttttcg gtcataatct aatgctaccc taatctctat gaatgacaag
     4081 cgcttacttc taatcagcaa ctcgacactc cacggcagcg gttatctcga tcatgcagaa
     4141 tccgagatcc gcagtttcct gggcgacgcg aaacgagtgc tgttcgtgcc gtacgcactt
     4201 ttcgatcgag ataagtatgc ggcaaacgcg cgacagcgtt ttcagaagat ggaatacgag
     4261 ctcacgtcgg tccacactgc tgctgatcct gtagcggcgg ttaatgaaac ggacgcggtc
     4321 ttcattggag gtggaaatac attccgtttg ttgaaggcgc tttacgaatt cgatctgatt
     4381 gaggcgattc gcaagcgtgt gtcgaaggga atgccgtaca tcggatcgag tgccggttcg
     4441 aatgttgccg cgccgacgat caaaaccacg aacgacatgc caatcgtgca gccgccgtcg
     4501 ttcaacgcgc tcgggttggt ttcatttcag atcaatcctc actatctcga tccggatccg
     4561 aattcaaagc acatgggaga gacgcgcgaa gagcggatcg ttcagtttct cgaggagaat
     4621 gacacgcctg tcgtggcttt gagagaaggc gcgctgctgc gaattgagaa tggtgagacc
     4681 atcttgcgtg gatcaacggg cgcgaggata tttcggaagg gatttgaaac agtcgaaaga
     4741 gctcctggtg cacggttgga cgatttattt tagccacgga tgacacggat tttaaggcgg
     4801 attttccaac cagccgccgc gaaagcccgc gcggcgcaga ccgctgacga tgaacggaca
     4861 tcgttgcatc aggcgccaga tgaaacccgt gcggtagttt tcgatcatca tgatgattgg
     4921 tccctggtcc agcccgtaat agccctgtga tatccaacct ttttgacctc cggaactgaa
     4981 cgtcgggtta tagctgcatt tgaatccata ctcattcgtg atttccggat agtctttgtt
     5041 caagcgaaac agagacggca gcacgatttc cggagcgaac ggcaaggatg ctgcgactgc
     5101 ccacggtgcg agcgtgccat catcaggtcc gtacggaatc gagcgcgctt tgtaatcgaa
     5161 gaagcgcacg cgacgaccgt tgacgcgttg ctctgccggt ccaggaccat cgctcgccgt
     5221 aatgccccag ctaaagcgac cgtaaccggc aaacttcttc ggattgcgta tcgcataggt
     5281 ttgctgtgca tagatcgctc ggcgggtatt ttcaaaataa tcgctggcct tgccgcgcat
     5341 gtaatcgtcc tggatgccac ggaagtcgat ccacatgtgc gacaggtgat gaataaacag
     5401 cggaccagcg taaacaaact cctgctcgta gagtttcttc cacttgtacg tcctggtcca
     5461 tgcctggtag ctttcggcag gcagtggaaa cgttgctgaa gcgagaccga gaacatagag
     5521 tatcagcgct tcactgtagc cggaccaacg atacctgatg aatcctcgct ccggtcgcca
     5581 accgtgtgaa accgttagcg cgccgtgctg cgcccagtgc cagtccgcgc gtttgtaaag
     5641 cgcttcggca agctcgcgaa cttcacgctc agcgggagtg tcagcgtcga aatagcacga
     5701 cgcggccaga gcgccggcga gaagataggt gctatcgatc gtcgacaatt cacatttgtt
     5761 cgcgcgacgc ccggtctcca tgtcgaggaa gtgataatag aagcctttgt aaccggtggc
     5821 gtctcgctgt tttccctgtg gcgcgtcgtg aaagaatcgg agggttgcca gagttcgctg
     5881 cactgcttcg gcacgggtca ggtacttccg ctcgaccgcg atcggataag acgtaagcgc
     5941 aaagccgacc gctgcgattg aagacggcga tccgcgacgc gtgctgtcac gcacgagccc
     6001 gttctcggga ttcacttcgt cggcgaagta tttgaaggtg tcgcgttgca gacgggccaa
     6061 gtcaacgtct gtcaacgacg gctctgacaa ctcgctcctc acagaacctg tcgcaccgcg
     6121 cgacgaccga gttcagccgt cactccatac acgagatgtg aagcgagcgc ataaacatgg
     6181 gtcgagaccg gatactcagt tggtcctttc gagagaccca acaaaggtac ggagatctca
     6241 tcagcgagta gccagaagcc ggcgccgaac ggcaagccgc ccgcagtcgt cacctgtgga
     6301 gtgacttcgg cgagtgcgcc atacaatccg cccgagagag tgccgaaggc gtaatgaaca
     6361 gcagcgcctg ctgattcctt ttcactctcc tgcaactcgt gacctaacac gtttttcgag
     6421 attgctttcg cagtttcgac ggtggcgtcg tcctgctcgg ctgtgtcctg ggcctgctcg
     6481 ccttgcgaac cttcggcggg ttgcatcgac tgtgcgccgt gcgatttctc ggtgccttca
     6541 gcgacgcgag tccaggcggc ttgaaactgg ttcatggtcc atgaagcgag gaggccgccc
     6601 gcgagtccgg ccaccatgcc tttcaaaagg tttgagtcgt tatcacgcat attcacctca
     6661 atcttcatcc ggatctcctg tgttcgagcg tagagtttct cgctgtgcaa cttcgttgcc
     6721 acgtcgattt tgttgagaaa tttagcagtt gaaggcgagg ggctgtacgt gactggtaca
     6781 aaggcgcaaa gaagagcaga ctcttctttg cgccttttgt gctttttcgt ggcttacttc
     6841 tcttctaaca ccgacagaat attccctgcc ggatctttga accacgcgat cattggacca
     6901 tgtgggccac gcgcgattcc gcgttcgtca gtcttgatgt cgggcaaatc ataatgttcg
     6961 agcttcacac ccaatccggt gagctcgtct accgctgcat cgatgtcgtc cactgcaaaa
     7021 ttcaggacgg tgaaagaggc cggggtatgg tttggtttgg gatacaagaa aaccgcgttg
     7081 tcgtccaagt ggagctcgag tccttccggt gactccttaa cgtcgagccc gagtgtttga
     7141 ctgtaaaact gttttgcctt ctgcaaatca ttcacagaaa aactcgagaa cgtctttgct
     7201 tccttgaaca tgtttgacgc tttctttctc cccggcgatg tgcctgcttt tgtactcata
     7261 caaatccccc ttcagtaatt atgtgagcta ttaaacaccc gaacgtttac aggcatgtcg
     7321 cagttatttg atttcgttat tactttttgg tggcatgccc ctttcgccgc gctttttacg
     7381 cgttagggta catgcagagg aaccgaagga ggtgttcccg acatgcttaa tcaatataca
     7441 taccaggcga ccctcgcggc gtcggagagc attcactggc gcgttgaaga catcatcgga
     7501 ggcgagaaac gtctcaactt cacgcagcct ttcatgcccg aagcgctggc gaatacggaa
     7561 ccgctgacgt ttctttcccg ggaagagaaa cgcattctta accagatcag aggcaatgcg
     7621 tacctctgca tcttcggact cgtagaagaa ttcatactgc cattcgtatt ggaccatgct
     7681 cgtccacagt tgcgcggtga cgattatcgc gtgcgcgcgt tgttacgatt cgcgagtgaa
     7741 gaagcgaaac atatccagct tttcaaacgc tttcgcacgg aatttgaaga cgggtttggc
     7801 acgctctgca acgtcattgg accacccgac gcgatcgcag ccgcgatcct ggtccacgat
     7861 ccactcgcga tcgcgatcac cacgctacac atcgaatgga tggtccagag tcattacctc
     7921 gacagcatca aggacgatca ggagctcgac gcgcagttca agagcctgct caagcaccac
     7981 tgggttgaag aagtgcagca tgcaaagctc gacacgttga tggttgaagc actcgcagca
     8041 gatcgcactg aagagcagat catgagtgcg gtggaagagt atctggagat cggcggtttt
     8101 ctcgatgggg gactccagca gcaagtcgag tttgatctca ccagctttga ggtggcgagt
     8161 ggtcgcaggt taactgacgc ggaagcgaac gagtttcgtc gcgtccaggt gcgggcaaat
     8221 cgttggactt atctcggcag cggtatgacg cacgcgaaag tgatcgagac gctcgagtcg
     8281 ttgacgccga aggcgcggca aagagtggaa agcgtctccc tggcgttctg ctaaaagcca
     8341 tccacagatt acacagattg aatctgtgta atctgtggat acaagcttta ggacttggtg
     8401 gcgcgaatgt gtaacgtcat cacgacgtcg tcgcggatga ggttatcagc agcgccggca
     8461 tagttgatcc cgaagtcttt tcgattgatg gaaaaactac tctcgacggt cgctgcatcc
     8521 ggcgtcacat tgatcgttgc cgggaatgtg accgccttcg tcacgccgtg catcgttaga
     8581 tttccagtga tggtgtgagt cgcgcctttt tcaccgcctg ccttgatggc ggtcgagacg
     8641 aacgacgctt caggaaactt cgccacgtca aagaaatcag cagtcttcaa atgcttcgtc
     8701 agatccggcg tgtcggtcgt gatcgaatcg gtcttgattg tgatattcac acgactcgtc
     8761 tccggattac ccgtgtagtc gacctgtccg gagaaatctc caaacgagcc attgtgcttg
     8821 ccggtaactt tcgatccgat aaactcgatc ttcgagttct gcgatgtgat ctgatacttc
     8881 tggcccggcg cggccgcagg cgaggccacg cgtgccgctt cacccgttct agccttcgtt
     8941 gtgtcggccg cgggatcgtt gcaagccgtg aaaacgatca accccaagag aattgtaatc
     9001 gcgatacatc tgcgcatcct gtatttcctc cttcttaaaa tggacgttta attgcgacaa
     9061 ccctgcgata atattcgtga gagtctgatg ttggccgaca caggagaaac gtcggctcga
     9121 tagatctgcc agccacggtc ctgcacattc gatcggcgca tagtgtaaca accggcaaat
     9181 acgcgctcgc tgccattgcg catcgtagac acaacgattg ccggaatttc agcataaaca
     9241 ctgcctgccg ctccatcgac gcgcgtcgat ggatcaacca gcaagcgaac ttgatccgtg
     9301 tcgttaaaac cgcgggagaa ctgttcatac gacgaaggcg gcgaatccca gaaacgaaaa
     9361 gcactacgat agtcacgcgc attgatcgcg ctgtaatacg ccgcgagtgt tgcgaccgga
     9421 tcgctgcgat cctcgcgtgg accatcctga ggttccgaaa tcgaattgtt cacaaaattc
     9481 agaacgctac ggccacggtc ggataggatc gtgagtcgac ccctggtaag acgaaacctg
     9541 ttggcggcgc cgagtgtgga taggaatctc tgttcctgct cgtttgcgtt ctggtccaaa
     9601 catgcacgtc tggtactgat gagccggctg aacgaaatat tgtctccacg cacctggtaa
     9661 gtaccgctaa aggagttgca gcctgtcgat ccactcgctc gattgtcttc tccaaacctt
     9721 agcgtcaccg tcgttcccgc aacgacgttg gtttcggcgc ctgctggtcc gagtgaaacc
     9781 agtcgccagt cgattccggt gagcacgcgc tgatctgttg acatactcct ccctgtttgc
     9841 ccgtaagctg caaaggacat catcagcaaa gccaccacga gactttgagc gcgcatatgc
     9901 ttacctccaa ccggaacccg gaagaataga acaaagccaa ccagtactga tcttgcgtta
     9961 caattacgtg catgttttat gacgttgctg ttattggtgc gggcgtcttt ggcgcctgga
    10021 gcgcttatca actcagactc accggcgcat cggtgctgtt gctcgacgct tacggaccag
    10081 gaaacagtcg cgcgagctca ggcggcgagt cgcggataat tcgtcttggt tatggaccag
    10141 acgagatcta ttcgcgctcg tcacaacgct ccttcacgct ctggcagcaa ctcttcgaaa
    10201 agaccgagcc gctgtttcat aagacaggcg tgctctggct cgcacgcgag cacgacgctt
    10261 actgcgaagc tacgctcaag accttacaac gtctcaacgc taactttgaa cggctggacc
    10321 aagatgaact aatccggcga tttccgcaac tcgaacttgg ggcaacgaag tgggcgatac
    10381 tcgagtcgga ttcgggcgtg ctgatggccc gacgtgccgt gcaagcggtg gtagcgcagg
    10441 cgcaggcgaa tggggttgag tacgttgaga aagcaatcgc gcctccgcaa ggacgactcg
    10501 attcgattcg tacggtttca ggtgaagcga tagaagcagg gcagtttgtg tttgcgtgtg
    10561 gaccatggct gccgaagctt tttcccgagt tgctcaagga tctgatctat gtgacgcgac
    10621 aggaagtgat ctttctcggt gtaccgccgg gagacgacag ttttaatcgc ggctcgatgc
    10681 cggcctggat cgatttcaac gatcttgttt atggattacc gaatgtggat ggtcgcgggt
    10741 tcaagatcgc gatcgatgcg catggcgcgg agttcgatcc ggatcgcgga gagcgagtga
    10801 ttagtgttga tggattgaag gcgatgcggg agtatgtcgc gaaacgagtg ccgcggctgg
    10861 cgcatgcgcc ggtgctggag acgcgcgttt gccagtatga gaacacatca aatggtgact
    10921 ttctgatcga caggcatccg gcgtttgaga atgtttggat tgttggtggt ggatcggggc
    10981 atggattcaa acatggaccg gtcgtcggtg agtacgtggc gcggatgatt gcaggcgagc
    11041 aggtacgaga acctcgcttt ggtcttgaaa gcaagcagcg ggttcaaaat cgagcggtgt
    11101 attagacagg attacaagat tcgcaggatt aacaagataa cctgttactc atgcaaatcc
    11161 tgtaatcctg taaaaagcga tcaccctgaa tctaaggctt ggcggtggcg ttgtagtctt
    11221 cgagcagctt ttgcgcgcga atactcggac tgatcagcgc cagcttctgc gaccagtcgt
    11281 agccactgaa aactcccacc acctcggcct ccgagttgta aaccggactt cccgaaaaac
    11341 cgtcgcggct atcgatcttg actgaccaat actcctggtt gccgagcatg tagttgccgt
    11401 aaaaactgcc gtggctgacg actttatccc cgtgcggccg cgcaatcaga aacagcttgt
    11461 cgtctctgtt cggcgctgtc tggaatttgg gggctttggc tgatttcgag ttacggcaca
    11521 tttcgagcag ggccagatcc gcttctttgt cgaccttgat tactttcgcc cgacagccgt
    11581 caacgaatac ttttacagaa agctcatcag tccctttaaa ccccagcatc acttttttgg
    11641 tggtgctgag attgccggag atcacgtgat aggcagtcat caccagacca ttgcctacca
    11701 ggaaaccggt cgcatacccg ttgggtccga catcgaacag aactgaaatc gcctgttcca
    11761 acgggttttg ctccttcttc gtgaactcca actccagcga caatgtatac cgctcctgtt
    11821 gcttgagcag cctgttttga cccttattac tgtcagccac acatacaggt gcaagcagaa
    11881 gtatggcggc catacaggta aggctgcaaa gcttactctt catcgttgac ctccgttcag
    11941 gttctaaaag tttgacgact tcgtccgcgt ggagggaaga cgaagtaaac caattgttat
    12001 tggactagaa agaaattgtc cgggactaag accggagcat tttaggcctc agcctgagaa
    12061 taggcaatga ttttagtgcg cggactgtga atcgtgagaa ttcagaaaca gtttacgcgg
    12121 atgaacgcgg attagaagac gatttaaatc cgatctgcgt taatacgcgg ctcaaactga
    12181 tgctgtcttt tgtctgggct gattgatgcg tgtgtaatcg ggatggaggt agaccaggcg
    12241 gccgccgact atggttgcca ctgcggcgcc gtgcatcgcg cgattgtgaa acggcgtgtt
    12301 gcggcttttt gatttcgagc gacttacatc gaagacccat tcgagatttg gatccagcaa
    12361 cgtcacgtcg gcatgcgcgt cctttttgag cgatccgcga tcggtgagtc cgaagatccg
    12421 ggcgggattc gtcgaacaca tctgcaccac gcgctcgaga tcgatcaacc cagcgtgtac
    12481 gagatcgaac gctaacccga ctgcagtctc caaacccgtg atcccgaacg gcgcctgatc
    12541 aaactcgagc cctttttcat caagatgatg tggcgcatga tccgtggcga tcgcgtcgat
    12601 tgttccgtct ttcatcgcct cgagaacggc gtcaacgtgt tccctgctcc gcaacggcgg
    12661 actcatcttt gtgtttgtgt cgtactctga aacagcctcg tctgtgagcg tccagtgatg
    12721 cggcgtcact tcgcagctca cattcaatcc ttcatgcttt gcgcgacgca ctgcttcgag
    12781 cgctccgcgc gtcgagacgt gggcgatatg aactttggct ttcgtcagtt tcgcgagcac
    12841 acaatcgcga gcaacatcta cgtcttcagc cgccgccggc ataccgcgca ggccgagaat
    12901 caatgaccag cgtccttcat gcattacgcc gcccgcgctc agtgactgat cctggcaatg
    12961 atcgaccagc gtcagatcga agccacgcgc gtattccagc gctcgtcgca tcatcgagga
    13021 agtaggcacc gaacgaccat cgtcagacac tgcgacgata ccggcgttct tcatctcgcc
    13081 catttccgcg agctcttttc cctggctcga ctttgtgacg gcgccgatcg ggaaaacgtt
    13141 tgcgagctgc gcggcgcgtc cctggtcgat gatgaagcgt gttactgcgg gattgtcgtt
    13201 cacgggatct gtgttcggca tcgcacagat gctggtccag ccgcccgcca ctgccgccga
    13261 tgcgcccgtc gccaccgtct ctttgtactc ctgacccggc tcgcgcaggt gtgtgtgcat
    13321 gtcgatgaag cccggcgcga cgatcaaacc ggtggcatca atcacgtgcg cttgttcggg
    13381 caccggctca ccgcgctcga gcagtccggc gatgcggccg tcttcaatca gcacgccgcg
    13441 cccgcccgtg ttcagattct gtgcggggtc gatcacataa ccatttgcaa tcagcagctt
    13501 catagtttcg atactttatc cagagatgac gcagattgtt taagacatct gtgtaatctg
    13561 tggatggttt tttatttcct gtccgccggc caaaaggtaa agcactgcca tgcgcaccgc
    13621 gacgccgttc gtcacctgat cgagaatcaa cgagcggctg ccgtctgcaa tctccgaaga
    13681 gatctcgacg ccgcgattca tcggacccgg gtgcatcacg atcgcgtcgg gcgcggcgcg
    13741 ctctagatgg cgcggttgta agccgtagtg aactgcgtat tcgcgcatcg aggggaaaaa
    13801 cgctgagtcc tgtcgctcgc gttgaatgcg caggatcatc accacgtccg cgccgtcgat
    13861 cgcgtcttca aagtgttttt cgatgtgcaa cccgctttcg accagctcgg caaactcgtc
    13921 cggcacgagc gtgcccggac caccaacact cacgtgcgcg ccgagttttg taagcaggta
    13981 gatattcgag cgcgccacgc gtgagtgcag gatgtcgccc acgatcgcga ctttcagacc
    14041 ttcgatccgt tccttgtgct cgcgaatcgt gagcgcgtcg agcagcgctt gtgtcggatg
    14101 ctcatgactc ccgtcgcccg cgttgataat cggcgcgtta gtcatgcgcg ccagatgctg
    14161 tggcgcgccg gctgacgagt gacgaatcac gatgcaatcg ggcgccatcg cttcgaggtt
    14221 gcgcgcggtg tcgagcagag tctcgccttt cgtgacgctg gatgttgaga cactaatatt
    14281 aattgcgtct gccgaaaggc gcttcgcggc gatctcgaaa gacgttctgg tgcgtgttga
    14341 cgactcgaag aagaggttga ttacggtgcg gcctcgcagc gcgggcacct ttttgatttc
    14401 tcgtttagaa acttcgcgaa aactctctgc ggtatcgagc aggtgaacaa tctctccggc
    14461 ttcaagctcg cggatgccaa gcagatggcg gcgattgaaa gttgcttcca tgtttatctg
    14521 gcacaaaaaa agcggcccga ggccgctttg ttaagccgtc tctgacggcc gctcgacgat
    14581 taccacgcct tcttcctcgt cacactcctg aagcatcacc tttatgatct cgctcttctt
    14641 tgtcgggacg agtttgccga tgtaatccgc ctggatcggc agctcgcgat gttctttgcc
    14701 gcgatcgatc agcacggcga gttgcacgcg acgcgggcga ccgaagtcga ttagttgatc
    14761 gagtgccgcg cgaatcgtgc gaccggtgta gagcacgtcg tcgatcagaa cgatgttgcg
    14821 gttttcgatg tcgccgggaa gatctgtgcg attgacgacg ggtttagcgc cgacagtcga
    14881 gagatcgtcg cgatagagtg tgatatcgag tacgccgacg ggcggtcgat agccttcgag
    14941 atcggcaatc ttctgcgcca tgcgctcggc gagtggaaca ccgcgacgtc tgattccaac
    15001 gagaaacaag tcgccggcgc cgtgattttc ttcgacgatc tcggacgaca ggcgcgccaa
    15061 tgccctattg atgcgcgggc catccatgat gcgggctttc tctacaaagt ccatgtgtgc
    15121 tggggatact accatagcca caaaaaggca caaaaggcgt aaagtgtatt tttgataaat
    15181 gtccgacaag ctaaagcatg tcggacatca aactgtgcct tttgtgcctt ttgtggcgaa
    15241 tctgtatgat gtcgatcctg aaatggtcta ccgttcactg gtacgcccgc tcctcttccg
    15301 tctccctgcc gagaccgctc acgaattggc tcttcattct ctttcgttat tacccgcgag
    15361 cttaatcacg aagcgcgtcg ataacccggc gcttagtagc aaacgattcg gcctaacatt
    15421 cgcgaatcca atcggactcg cagcgggttt cgataagaac ggcatagcgt tacaaccgct
    15481 cgcggcgctt ggttttggtt ttattgaagc tggcacagtt acatatcacg cacaacccgg
    15541 taatccacga ccacgactct ttcgactctc cgaagatcag gcgttgatta atcgcgccgg
    15601 cttcaataac gaaggcgccg ccgcgttctc gcgcagagtc tcaaatcatc gacccgactg
    15661 tgtgctcggc gtcagcatcg gtaaaagcaa gatcacacca ctcgaaaacg cgaccgaaga
    15721 ttatctggcg agcttcgagc tcgttcacga ggtcgccgat tacatcgcga taaatgtcag
    15781 ctcaccaaac acgccacagt tgcgcgagct acagcaatcg cagccactcg aatcactact
    15841 ttcagcgctc cagagccgcc gccgagagtt gcagaaacac gttcctttac tcgtcaaact
    15901 ctcacccgat ctcgatcgtc gcgaactcga aaccattgtc gaggttattg aacgtttgaa
    15961 tatagacggc atcatcgcga ctaacacgac tgtccgtcgc gaaaatttac gaactaatat
    16021 gaagcgtgtc gcagcgtgtg gtgaaggggg attgagtggc agacctttaa aggacacatc
    16081 cacacggatg atcgcgacgc tctatgagct gaccggcgga cgcat
//
LOCUS       DASSFQ010000099         5805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_936253, whole genome shotgun sequence.
ACCESSION   DASSFQ010000099 DASSFQ010000000
VERSION     DASSFQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936253"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1661
                     /locus_tag="VFH96_03205"
     CDS             <1..1661
                     /locus_tag="VFH96_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6973002.1"
                     /translation="MAHLKITDASGRQWQFSFLPNVACTIGRAPDNTVVLDDPRASRY
                     HAHINQSENGNFTIVDGAVINGQLRRSANKVFINGDPRFDHQLKNGDRITIGASTLRF
                     EQPVEERTTDVRYDDKPLGHTQLLISANDVMSTVLRSKDEITTSPQRDKVLESLQRKA
                     NILSALYEMSKTLGSVFDLNAIFEKATDIIFRSTPADRVVALIAEDGREDANLTPIAM
                     RARDNKLEAHARKLSIGRTITRKVMKERVALLSQDAASDEQFAGVDSIVSQGVRSTIC
                     APLVAESRVHGALYADRLDPFAAFKPDDLELISAVAAQTAIAVENARAHERLAREEVA
                     RANYSRFLPEYVVKQMLENPESFKLGGVSQTITILFADIRGFTRISEHAPPEKIVSLL
                     NRYFSAMTDIIFAHGGTLDKYLGDGLMALFGAPTATPDDASNALNAAVAMQRRLLGIN
                     RELRDEGIPEIGVGMGLHTGEVVVGYIGSERRSEYTAIGDTVNTSSRLESNARGGEIL
                     ISDATAQAAHSRYKLKPREAIMVKNRQQPVNLWEVDWQRASGSF"
     gene            1789..2892
                     /locus_tag="VFH96_03210"
     CDS             1789..2892
                     /locus_tag="VFH96_03210"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6973003.1"
                     /translation="MSFIGKTKTLLGLNALKNLVSDSMAIDMGSASTIISVRGRGIVV
                     DEPSVVALNKVTGEVIAVGEEAHKMQGREARDVTVIAPLVDGVVADFERTRTMLDHFV
                     KAARSGTSHFSRRAIMSVLSEVTQVEQRALLAAAEEARIRTVYMIEEGQAAAIGAGVP
                     ISDERAAAVVDIGGGTTNVAAIVSGSIIASRAERMGSSDIDVAIMDRLRRHRGLSIGV
                     QTAERLKLELSSAIEPADPDRKINVRGRDVQTGSPGAVDISAGEVYTITVPIIRRIAE
                     EVRTLLGDLSPEVAGDIFDRGLILTGGGAQLEGLPSYLQKELDLDVSVAEDPRFAIVR
                     GLAQLYDEPLLLRRVARTEPHPLIDTEATAFET"
     gene            2928..4070
                     /locus_tag="VFH96_03215"
     CDS             2928..4070
                     /locus_tag="VFH96_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6973004.1"
                     /translation="MNVLDFSKRQITVAHIFGIPVRFDYRWFIVFFFSVWLIAVNLSR
                     GGMWVGSYKLMPVGVAAAWVLAIITTVGLFLSVLGHELSHALMGRAEGIEIEEIVLHP
                     FGGLARLRNEPDSPRAEFRIAVAGPAASFLFAAVSFVLMLPAMMTGYTIVGGVLLLLC
                     AGNLLLAIFNLFPGYPLDGGRVLRAILWRRTGDIKEATRLAGICGMLIAAILIIFGVY
                     MAIAPGFRSYFMGFWSVLVGLFLFDAAYSVVKHVRGRVRNIVSEAMSAPFAIEPDLLI
                     SKLIDSVLPMHRQVAFPVAQNQRLHGMLSLEDLKGLPRDRWHLTKARDIMRPIAPRFF
                     VEPNATLDYAQELMKRNGIGSVAVVGKTGELVGFLQSGKIKRKKRS"
     gene            4132..5079
                     /locus_tag="VFH96_03220"
     CDS             4132..5079
                     /locus_tag="VFH96_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6973005.1"
                     /translation="MLAVIGALFANGLIAILKLAAALITGSSGMMAEALHSVADTTNQ
                     VFLLLGLRFYKRPASEKHPFGYGKERFFWSFIAAIFIFGVGATYAIYEGIEKLAHPHA
                     PTNLGWAYGVLAISFVLESGSIALAIYQEVKEAHHEGLTFGEYLRESKDPTAKTVLFE
                     DSAALIGIVIAATGLVLTDYQVGGAAGAYWDGLASIIIGLVLAVVAFVLARSSRGLLL
                     GEAANPKVLAAIKQAIESHENVERVVELLTMHLAPKHILINAHVKLRPELRTDDIVKS
                     IEEIEDRIKSAEPKVEMIFLEAARDDQADKHQAVAEHIG"
     gene            5135..>5805
                     /locus_tag="VFH96_03225"
     CDS             5135..>5805
                     /locus_tag="VFH96_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HET6973006.1"
                     /translation="MLLLLPYTEYLRRMIEPRNVADIVFVFLLVYAVLKLLRGTRAAP
                     MAAGIGFIIFLYWLSIKQDLATLEFVLSNGMLYIGIVIIVLFQSEIRQALITFGNRFR
                     VPFSRHRGQFGEGVYDEVILAATTLASTKTGALVVIERNVGLKNVTDGGVQLDAELSY
                     DLLISIFNTASPLHDGAVVVRRHRIAAASCFLPLTLNPRLSRDLGTRHRAAIGVTEDT
                     DAVAVV"
BASE COUNT         1254 a         1579 c         1644 g         1328 t
ORIGIN      
        1 ccatggctca tctaaagatc accgacgcta gcgggcgaca gtggcagttc agttttctgc
       61 caaacgtcgc ttgcacgata ggtcgggcgc ccgacaacac ggtggtcctg gatgatccac
      121 gcgcttctcg ctatcacgca cacatcaatc aatccgagaa cggcaatttc acaatcgtcg
      181 acggcgcggt tatcaacggc cagctgcgac gcagcgccaa caaggtcttc atcaacggcg
      241 acccgcgctt cgatcaccag ctcaaaaacg gcgatcgcat cacgatcggc gccagcacac
      301 tgcgttttga gcagcccgtc gaggaacgaa ccaccgacgt ccgctacgac gacaagccgc
      361 tgggccacac gcagttgctg atctctgcaa acgacgtgat gtcaaccgtg cttcgttcta
      421 aagacgaaat aacaacgtcg ccacaacgtg acaaagttct cgaaagtctg caacgtaaag
      481 ctaacattct tagtgcactc tacgagatga gcaagacgct cggctcggtc ttcgatctca
      541 acgcgatttt tgaaaaggcc accgacatca ttttccgctc gacgccggcg gaccgagttg
      601 tggccctaat cgccgaggac ggacgcgaag acgcgaatct gacgccgatt gcgatgcgcg
      661 cgcgtgacaa caaactcgag gcacacgctc gcaagctttc gatcggtcgc acgatcacgc
      721 gcaaggtgat gaaggaacgc gtcgcgctgc tctcgcagga cgcggcttcc gatgaacaat
      781 ttgccggggt tgattcgatc gtctcgcagg gagtgcgctc gacgatctgt gcgccgctcg
      841 ttgcggaatc gcgtgttcac ggtgcgttgt atgccgatcg gctcgatccg tttgcggcgt
      901 tcaagcctga cgacctcgag ttgatcagcg ccgtcgctgc tcaaaccgcg atcgcagttg
      961 agaacgcgcg cgcacacgaa cggctcgcgc gtgaggaagt tgcgcgagcg aattacagcc
     1021 ggtttttgcc cgagtatgtg gtgaagcaga tgttggagaa ccccgagtcg ttcaagctcg
     1081 gcggcgtcag tcagacgatc accattctgt tcgccgacat tcgcggattc acgcgcatct
     1141 cggaacatgc gccgccggag aagatcgtca gtctgctcaa tcgttacttc tcggccatga
     1201 ctgacatcat ctttgcgcac ggcggcacac ttgataagta tctcggcgat ggtctgatgg
     1261 ccctcttcgg cgcgcccacg gcaacgcccg acgacgcatc caacgctttg aatgccgcag
     1321 tagcaatgca acgccgcctg ctgggaatca atcgcgagct gcgcgatgag ggtattccgg
     1381 agattggcgt cggcatgggc ctgcataccg gcgaagtggt cgtgggttat atcggctcgg
     1441 agcgacgctc ggagtacacg gcgatcggag atactgtgaa cacttcgtcg cgattggagt
     1501 ctaatgcacg tggcggcgag attctgatca gcgatgcgac cgcgcaggca gcgcacagcc
     1561 gttacaaatt gaagcctcga gaggcaatca tggttaagaa tcgccagcaa ccagtgaatc
     1621 tttgggaggt tgattggcaa cgagcaagcg gttcgttttg atttctctgt gcaaggtctg
     1681 tgttctctgt gtctctgtgg ttaggggaaa cgcagcatac aacaaccaca gagacacaga
     1741 gaacacagag gttgcacaga gaagtccttc atagggtcat tttttgctat gtcgttcatc
     1801 gggaaaacta aaaccctcct tggcctcaac gccctcaaga atcttgtctc ggattcgatg
     1861 gcaatcgata tgggttccgc cagcacgatc atttccgtgc gcggtcgcgg gattgtcgtc
     1921 gacgagccat cggtcgtagc gctcaataaa gtcactggtg aagtcatcgc ggtgggcgag
     1981 gaagctcaca agatgcaggg acgcgaagca cgcgacgtta ccgtgatcgc gccgctcgtc
     2041 gacggcgtcg tcgccgattt tgaacgcacg cggacgatgt tggaccactt tgtcaaagcg
     2101 gcccgcagcg gtacgtccca tttcagccgt cgcgcaatca tgagcgtgct ctcggaagta
     2161 acgcaggtcg agcaacgagc tttgctcgcc gctgctgaag aggcgcgtat caggactgtg
     2221 tacatgatcg aagaaggaca agcggcagct atcggcgccg gggttccgat cagtgacgaa
     2281 agggctgcgg cagtggtgga tatcggcggc ggcacgacaa acgtcgctgc gatcgtgagc
     2341 ggttcgatca tcgcgtcgcg cgccgaacga atgggtagtt ccgatatcga cgttgcaatc
     2401 atggaccgtt tgcgtcgcca tcgtggactc agcatcggag tgcaaacagc cgaacgactc
     2461 aagctcgaac taagctctgc gattgagcct gccgatccgg atcggaagat caacgttcgt
     2521 ggacgtgatg tgcaaaccgg cagccccggc gcggtagaca tctcagctgg tgaggtctac
     2581 acgatcacag tgccgatcat tcgacgtatt gctgaagaag tgcgaacgtt gctgggagat
     2641 ctgtcgcctg aagtcgcggg agatattttc gatcgcggat tgattctgac cggcggcggc
     2701 gcgcagctgg aaggactgcc gagctatttg caaaaagaac tcgatctcga tgtttcagtt
     2761 gctgaagatc cgcgcttcgc gatcgtgcgc ggattagccc aactctatga cgagcctctg
     2821 ttacttcgcc gcgttgcgcg aaccgagccc caccccctga tcgacactga agcgaccgct
     2881 ttcgaaactt agtcttggac aggattcaaa agatttttca ggattaaatg aacgttctgg
     2941 atttctctaa acgtcagatt acggtggcgc acatcttcgg gattccggtg cgtttcgact
     3001 atcgctggtt catcgtcttc tttttcagtg tttggctgat cgcggtgaac ctcagccgcg
     3061 gcggcatgtg ggttgggtct tacaaactga tgccggtcgg cgtcgcagct gcgtgggtac
     3121 tcgctataat aacaaccgtg gggctgtttc tttccgtgct tggacacgaa ctctcacacg
     3181 cgttgatggg ccgggccgag ggaatcgaga tcgaagagat cgtcctgcat ccatttggcg
     3241 gactcgcccg tctgcgcaac gaaccggata gtccgcgcgc tgagtttcgc atcgctgtcg
     3301 ctggaccagc agcgagtttc ctttttgcgg ctgtaagttt tgtgttgatg ctgccggcaa
     3361 tgatgacggg atatacgatt gtcggcggcg tgctgttact actttgcgcc ggcaatctgt
     3421 tgctcgcaat cttcaatctc tttcccggtt atccactcga cggcggacgc gtgctgcgtg
     3481 cgattctgtg gcgtcgcacc ggtgacatca aggaagccac gcgactcgcc ggaatttgcg
     3541 gcatgctgat cgccgcgatt ctgatcatct tcggcgtcta catggccatt gctcccggtt
     3601 tcagatccta cttcatgggg ttctggtctg tcctcgttgg cttgtttctt ttcgacgccg
     3661 cctacagtgt ggtgaaacac gttcgcggac gagtgcgaaa catcgtcagt gaagcgatgt
     3721 cggcaccttt cgcaatcgaa ccggatctcc tgatcagcaa attgatcgat tccgtcctgc
     3781 cgatgcaccg ccaggttgcg tttccagtcg ctcagaacca gcggctgcac ggaatgttgt
     3841 cgctcgaaga tctcaaagga ttgccacgcg atcgctggca cctcaccaaa gcacgcgaca
     3901 tcatgcgccc aatcgccccg cgtttctttg tcgaaccaaa tgcaaccctc gactacgcac
     3961 aggagttgat gaaacgaaac ggcattggct cggtcgctgt ggttggaaaa acgggcgaac
     4021 tcgtcggttt tctgcaaagc ggaaaaatca aacgcaagaa acgttcataa acttttcaca
     4081 aattacatag actgttttac agaagctgag aaatctgtgt aatctgtgga tatgctggct
     4141 gtcatcggcg cgctctttgc caacggattg atcgcgatac taaagctggc agctgccctg
     4201 atcacgggct cctccggcat gatggctgag gcgctgcact cggttgccga caccacaaat
     4261 caggtgtttc tgttgcttgg tttgcggttc tacaaacgac cggcctccga gaagcatcca
     4321 ttcggttacg ggaaagaacg gtttttctgg tctttcatag cagctatttt catttttggc
     4381 gttggcgcga cgtacgcgat ctacgagggc atcgaaaaac tcgcgcatcc gcacgcacca
     4441 acgaatcttg gttgggctta tggagtgctc gccatctcct ttgttttgga gagcggctcg
     4501 attgcactgg ccatttacca ggaagttaaa gaggcgcatc acgaaggcct gacctttggc
     4561 gaatatctgc gcgagtcgaa agatccgaca gccaagacag tgctctttga agattcggcc
     4621 gcgctgatcg gaatcgtgat cgctgcgacc ggacttgtgt taaccgacta tcaggtcggt
     4681 ggcgccgccg gagcgtattg ggacggactg gcttcgataa ttatcggcct ggtgctggca
     4741 gtcgtcgctt ttgtactggc gcgttcttcg cgtggcttgt tgttaggtga agcagccaac
     4801 ccaaaagtgt tggcggccat caagcaggcc atcgaaagtc acgaaaacgt cgagcgcgtg
     4861 gttgagcttc taacgatgca cctcgcgcct aaacacatct tgatcaacgc tcatgtgaag
     4921 ttacgacctg agctgcgtac tgacgatatt gtgaagtcga tcgaagaaat tgaagaccga
     4981 atcaagagcg ccgagccaaa agtggaaatg atctttctcg aggcggcacg cgacgatcag
     5041 gctgataaac atcaagccgt tgcagaacac ataggttgaa gtttttgcac ggatttcacc
     5101 ggatgaacac cgataacaga aatctgcgga agagatgtta ctgctactgc catacaccga
     5161 gtatttaaga cggatgattg agccgcggaa tgtcgcggac atcgtcttcg tcttcttgct
     5221 cgtgtatgca gtcttgaaac tcctgcgcgg tactcgcgcc gcgccaatgg ccgccggcat
     5281 cggcttcatc attttcctct actggctttc catcaaacaa gatctcgcga cgctggagtt
     5341 tgtgctcagc aatggaatgt tgtacatcgg catcgtgatc atcgttctgt tccagtctga
     5401 aattcgacag gctttgatta cgtttgggaa ccgctttcgc gtcccgttct cgcgacatcg
     5461 aggacagttt ggcgagggtg tctacgacga agtgattctg gctgcgacca cgctcgcttc
     5521 gaccaagacc ggcgcgctcg tcgttatcga acgaaatgtc ggtttgaaga atgtgaccga
     5581 cggcggcgtg caactcgatg ccgagctcag ttacgacttg ttaattagta ttttcaacac
     5641 tgccagtccc cttcacgatg gagcggtggt cgttcgccga catcgcatcg ccgccgcttc
     5701 ctgcttcctt ccattaacgt tgaatccgag gctctcaaga gatctcggca cgcggcatcg
     5761 cgctgcaatt ggtgttaccg aggacactga tgccgtggct gtagt
//
LOCUS       DASSFQ010000100         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_635380, whole genome shotgun sequence.
ACCESSION   DASSFQ010000100 DASSFQ010000000
VERSION     DASSFQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635380"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="ccmI"
                     /locus_tag="VFH96_03230"
     CDS             complement(<1..771)
                     /gene="ccmI"
                     /locus_tag="VFH96_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HET6973007.1"
                     /translation="MILFWVICALFIVIALAFVLPPALQRGEESNVVSDEERKQANIA
                     VYRDQLSELEADRRNGIVAEDQYAQDRDEIERRLLEDTATARSKKTTGAAPINARNTA
                     YLLGIGIPLVAIVFYLRIGEPNLITNPAPIGAPPSAASAAPPERTQEQIEANVAKLAE
                     RLQSNPNDAQGWTMLARSYSSMEKYSEAANADAKATELNPKDADLLAEYAFVTGMAGG
                     RSLEGKPTEIINRALKVDPENPKALQLAGSAAFQAKDYK"
     gene            complement(768..1253)
                     /locus_tag="VFH96_03235"
     CDS             complement(768..1253)
                     /locus_tag="VFH96_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HET6973008.1"
                     /translation="MTRLRLLPILLIFLLAPFVKAQVQEEEVPSDDPVIEQRLQNLSK
                     ELRCLQCQNQTLADSPAGLAADLRREIRIQMKAGKSDKEIIAFLTQRYGDFILYKPRV
                     TYTTYLLWFGPFVLLVGGLYLLFHYIKQRRDLITEKPLSADEHRRAEELLASLPGKET
                     T"
     gene            complement(1250..1786)
                     /locus_tag="VFH96_03240"
     CDS             complement(1250..1786)
                     /locus_tag="VFH96_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET6973009.1"
                     /translation="MLRYLIPLVIFIVLAVFLAIGLTRDPHELKSVLINKPTPAFRVP
                     QLKAADKMISNEDMRGKVWLLNVWASWCVACREEHPYLIEYAKSGVVPIYGLNYKDRR
                     EDALATLDELGDPYVVSAVDLDGRVGIDFGVYGAPETYIIDQGGTIRFKYVGPMMPDV
                     WKEKILPVVQELNRGTTK"
     gene            complement(1822..>2098)
                     /locus_tag="VFH96_03245"
     CDS             complement(1822..>2098)
                     /locus_tag="VFH96_03245"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HET6973010.1"
                     /translation="SGNVMTETAIDSGILRHLYISLGEPVGGGAWSVRVYYKPFVGWI
                     WFGAVLMALGGGLAVSDRRYALAARKEREAREIAKPIPSTVAASVTE"
BASE COUNT          443 a          574 c          600 g          481 t
ORIGIN      
        1 cttgtaatct ttcgcctgga acgcggcact gccggcgagc tgcaacgcct tcgggttttc
       61 cggatcgact ttcaaagcac gattaatgat ctcggtgggc ttaccttcga ggctcctgcc
      121 tccagccatg cccgtgacga atgcatactc agcgagtaga tcggcgtcct tcggattcaa
      181 ctcagtagct ttcgcatccg cgttggcagc ctcgctgtac ttctccatcg agctgtaaga
      241 acgcgccagc atggtccaac cctgggcgtc gttaggattc gactgcaaac gctcggcaag
      301 cttcgcgacg tttgcttcga tctgttcctg cgtccgctct ggtggggcag ccgaggcggc
      361 actcggtggc gcgccgatgg gagcaggatt cgtgatcagg tttggctcgc cgattctcag
      421 gtaaaagacg atagcgacga gaggtattcc gatacccagc aagtatgctg tattgcgagc
      481 gttgatgggc gcggcgcccg ttgtcttttt cgatcgcgcg gtggccgtgt cttcgagcag
      541 gcgacgctcg atctcgtcac gatcttgtgc gtactgatct tcagcgacaa tgccgttgcg
      601 ccggtcggct tcaagctccg agagttgatc gcggtagact gcgatgttcg cctgtttccg
      661 ctcttcatca gaaacgacgt tcgattcttc accgcgctgt aatgcaggcg gcaacacgaa
      721 cgcgagcgcg atcacgatga acagcgcgca gataacccag aaaagaatca tgtcgtctcc
      781 tttccgggaa gagaagcgag cagctcttcg gcgcggcgat gctcgtcagc gctcagaggc
      841 ttttcagtaa tcaaatcgcg tcgttgcttg atgtagtgga agagcaggta caagccgcca
      901 accagtaata caaaaggtcc gaaccagagc aggtaagtcg tgtaggtaac gcgcggtttg
      961 tacaggatga agtcgccgta acgctgtgtg agaaaggcga tgatttcttt atcgctctta
     1021 ccggctttca tctgtatgcg aatctcgcgg cgcaagtctg cggccagtcc ggccggcgaa
     1081 tcagcaagag tctggttttg gcattgaaga catcgcagtt ctttcgagag gttttggagt
     1141 cgctgctcga tcaccggatc atcgctcgga acttcttcct cctgcacttg cgccttaaca
     1201 aatggcgcga gcaggaagat cagcaatatc ggaagtagac gcaagcgagt catttagttg
     1261 ttccccgatt taactcctga accacgggga ggatcttttc tttccagacg tcgggcatca
     1321 ttgggccaac gtatttaaat cgaatcgtgc cgccttgatc gatgatgtaa gtctcaggcg
     1381 ctccataaac tccgaaatca atgccgacac gaccgtcgag atcgaccgcg ctcaccacgt
     1441 aaggatcgcc cagttcgtcg agcgttgcga gtgcgtcttc gcgacggtct ttgtagttga
     1501 gaccgtaaat cgggacgacg cccgatttgg cgtactcgat caaatacggg tgctcttcac
     1561 gacaggctac acaccacgac gcccagacgt tgagcagcca cacctttccg cgcatgtctt
     1621 cgttggaaat catcttgtcc gcggctttca gttgcgggac gcgaaacgcg ggtgtgggtt
     1681 tgttaattaa gactgacttg agctcgtgcg gatcgcgcgt cagcccgatc gcgagaaaga
     1741 ctgcgagaac gatgaatatg actaacggaa ttagataacg aagcataact ttagccgcgg
     1801 atctacgcgg atgaacgcgg attattcggt tactgatgcg gcgactgtgc tcgggattgg
     1861 cttcgcaatc tcacgggctt cgcgttcttt gcgtgcggcg agggcgtatc gtcgatcgct
     1921 gactgcgagt ccgccgccga gcgccatcaa aaccgcgcca aaccagatcc agccgacgaa
     1981 cggtttgtaa tagacgcgca cgctccatgc gccgccgcca accggctcac cgagagagat
     2041 gtagagatga cgaaggatgc cgctgtcgat ggccgtctcg gtcatgacgt taccgctg
//
LOCUS       DASSFQ010000101        13122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1818471, whole genome shotgun sequence.
ACCESSION   DASSFQ010000101 DASSFQ010000000
VERSION     DASSFQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818471"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFH96_03250"
     CDS             complement(<1..229)
                     /locus_tag="VFH96_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET6973011.1"
                     /translation="MKRRHKRIAFIVVGLAGLSLAAYLAASAFRNNLVFFFSPTQVAA
                     KEAPVGRTFRIGGLVQEGTVKREPDGLTVRFI"
     gene            complement(213..410)
                     /gene="ccmD"
                     /locus_tag="VFH96_03255"
     CDS             complement(213..410)
                     /gene="ccmD"
                     /locus_tag="VFH96_03255"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HET6973012.1"
                     /translation="MNWPDLSMGGYGFYVWGSYIASLIFFVWEVVSLKQRKKALTQQQ
                     QQSSKPTSTPVLRGSINETTS"
     gene            complement(413..1138)
                     /gene="ccmC"
                     /locus_tag="VFH96_03260"
     CDS             complement(413..1138)
                     /gene="ccmC"
                     /locus_tag="VFH96_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HET6973013.1"
                     /translation="MNLYKYASPAMFYPLAGKLIPWFAIPAALLFATGLFVGFFIAPT
                     DFQQGDAYRIIFIHVPAAWMGMFLYVLMAIYAGIGWAFNARLSSMMASSLAITGAMFT
                     AISLITGAFWGKPTWGTWWVWDARMTSTLILLFLYIGFISLQAAIDDPRRADRAGAVL
                     ALVGVINVPIIYFSVQWWNTLHQGATIRLLQKPTIRGSMLAALLIMLAACWLYSVAVV
                     LIRLRCIILEREQNAARFAEVGV"
     gene            complement(1183..1866)
                     /gene="ccmB"
                     /locus_tag="VFH96_03265"
     CDS             complement(1183..1866)
                     /gene="ccmB"
                     /locus_tag="VFH96_03265"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET6973014.1"
                     /translation="MNAPSSLSMLRWIVWRDLMLAWRRRADMLSTVFFFVIVVSLFPL
                     GIGPETQLLRTIAPGVVWVAALLASMLSLGRLFANDYQDGTLEQLLLTPQPLFLVVLG
                     KILAHWIVAELPLVLIAPLVGVQFDLSRETLWILFVSLLVGTPVLSLIGSVGAALTLG
                     LRGGGVLISLLVLPLYIPALIFGAGAVEASVMGTSPSAHLSLLGAFAIVSLLFSPWAT
                     AAALKISLE"
     gene            complement(1863..2546)
                     /gene="ccmA"
                     /locus_tag="VFH96_03270"
     CDS             complement(1863..2546)
                     /gene="ccmA"
                     /locus_tag="VFH96_03270"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HET6973015.1"
                     /translation="MSRERKLTNRPQSVTIAAEDDKILEVSKLGCVRGDRRLFSELDL
                     SVSPGTFIQLTGPNGSGKTSLLRILCGLLTPAEGKISWNDADIRSLGEEYVAQVTYLG
                     HRHGIKDELSAVENLRISNALNGVSVSKERAAEALAQMGLAGRESLPARMLSEGQRRR
                     AGLARLLVCDTKLWLLDEVLTSLDKGAVSLVRSLIENHVAGGGIAIVATHQDLELPAG
                     HTKRLELTT"
     gene            complement(2521..2958)
                     /locus_tag="VFH96_03275"
     CDS             complement(2521..2958)
                     /locus_tag="VFH96_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6973016.1"
                     /translation="MSTVKHSILALIIVGVTAIVAHAQDASNIAMISADELKTKVTSN
                     HPVTIIDVRSSEGYAASSTTIKGALHFKVRKLKSRLTYPPFKDLPKDREIITYCACPK
                     DQSSIAAAQILQAAGFKRVKVLQGGWQEWLRVSGPVEPRAKTD"
     gene            complement(2976..3668)
                     /locus_tag="VFH96_03280"
     CDS             complement(2976..3668)
                     /locus_tag="VFH96_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973017.1"
                     /translation="MMRIVILLIALTFCACSSAKRPAVEAPPVAYQPSPQSTPPVQLT
                     SVAGPKQSDVQEAVKRVFKDAAVIDTNYNPSFLSGDFNGDGSQDLAVILKPAKLDLMN
                     QELPPWLVRQPRNHKASRAAAPIEKDETFLAVIHGFGANHWRDPDATQTFVLKDVVGQ
                     NLKVHSLNEFVTANSGRKLPRPQGDLIGETVGGTPGYLYFAQATYSWYDPKTFDDAQN
                     APGAFHKSRTMK"
     gene            complement(3822..5156)
                     /locus_tag="VFH96_03285"
     CDS             complement(3822..5156)
                     /locus_tag="VFH96_03285"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET6973018.1"
                     /translation="MRKVLPVLTLVLFAVALVVPLSEPQPVIGQAATEALTTDMDAKT
                     DDLFNGFGVKGTPIVECVNEPVAPTARGNGRFEDNKFIFSERETIEDGLGPTYNDVGC
                     VECHQSVDVGAFGQQMEFRAGHITNGAFVDAPGGQLIHARGTDSDVVEHISTAETVKA
                     FRVTLSTLGDGYVEALANQTLQDNVAAQPVAQRGTLTTVPVTEAGNALRIGRFGWKAQ
                     QASLLSFSGDAYLNEMGITNPFDGFGGRSSDAADAGTHENPASTAEGVINVTFPSAFD
                     PVPDPEDDGDDVLAFADFMAATRAPGRQNPIPAAATRGDSLFNAVGCNVCHTRTFVTA
                     APGTLINGGAFTVPAALGNKIIHPFSDFALHNIGTGDGIVQNGGQGSANQIRTPPLWG
                     IRARNRLMHEGLNVTIFDSVQLHAGQATTARNNFNALTAAQRNDLIAFVLSL"
     gene            5528..7513
                     /gene="bchI"
                     /locus_tag="VFH96_03290"
     CDS             5528..7513
                     /gene="bchI"
                     /locus_tag="VFH96_03290"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0010007 - magnesium chelatase complex
                     [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HET6973019.1"
                     /translation="MGRLNRKTYPFTAIVGQEEMKLALLLNVVDPLIGGVLIMGHRGT
                     GKSTAVRALADLLPQIDVVAGCPYNCDPKDDLCDQCRGSEITSKKMLVPVVELPLGAT
                     EDRVCGTIDIERALSAGRKAFDPGLLARANRGFLYIDEVNLLEDHLVDLLLDVAVTGI
                     NKVEREGVSVEHPASFVLIGSGNPEEGELRPQLLDRFGLHAEVTTENYLQNRIDIIER
                     RDGYDRDREAFCKSYEADQEQLRKRITRARANLTKITVERPVLERIAQLCADLKVDGH
                     RGELTIMRAARALAAFEGRRAVTDDHVKRVSAMALRHRLRRDALDETATSEQIQQAVD
                     EVFPNPPQPSQSPGNGGGDTQNLDRPGKVNKDAPRQRRSASGASSRPNDADVLSPPAV
                     ERKSGEVKLEEQMRSTDRADKSRLLSRRASGGKAALVQQRGRYTRAVTFKSSGARIAL
                     DATLRALVSLEKRALPVSTQALRYKLLKHKRGTLFVFAIDASGSMAANRIARAKSTIL
                     KLLRKSYLNRDSVAIVSFHGTTANVDLPPSRSILRARRVLDSLRMGGSTPLGLGLVTT
                     IELLELVGNKFGETVVLLFTDGRSNVPLRRGGLNLRAFRQVKIESELRELTVALQRTK
                     ARVVVVDTQREFESAEETRRLASILNARFVKIAPANP"
     gene            7519..9153
                     /locus_tag="VFH96_03295"
     CDS             7519..9153
                     /locus_tag="VFH96_03295"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HET6973020.1"
                     /translation="MAKSANEERDAKVFALYEQLVEIEQRLIPTGLHVFGRPSSAGEK
                     TDLLKMIASFDRPETGARAIPEELLDDSVREFTANGVAAAVSFLEQKGLSAAATRPVF
                     TLLESVSTKLKTNNEIDSLLRALRGEFIEPGPGADIIQNPDILPTGRNTHAINPYGVP
                     SAAAFARSENVASSLLERYKNENGRYPEAMALVLWGLDNIKTQGEGVAQALWLLGTRP
                     VRDALNRATSVEAIPLAELGRPRIDVVMTVSGIFRDLFSPTVQLLDKAVRLVAQLDEP
                     LELNYVRKHVLQQVEETPCDLDEAAVRVFSNAPGNYGTNVNFMVMDSQWDREETLGDL
                     FVTRKCFAYGRDSKGRAFEGREARVAMDRALSRVEATYQNIDTFEIGLTDVDHYFEYL
                     GGVSKAVETRAKARPAIYLSDSLSREVKIRSLEETVRLETRAKTLNPKWYEGMLKHGF
                     RGVAEIENHVSNTFGWSATADAVDDWVYTEVANTFLFDEQMLERLRDLNPHSAHSLAQ
                     RLLEANGRGYWDVDASVLDKLREISRDLEDQLEGVA"
     gene            9170..11209
                     /locus_tag="VFH96_03300"
     CDS             9170..11209
                     /locus_tag="VFH96_03300"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HET6973021.1"
                     /translation="MKITILYVGSSLLAPLKQAEREINRAYKLDLSVAAYNFGSPLSD
                     SEWVEVEEDLKKSNIVFVIHVMDGENATRLVSALDKVRNDAVVVINCMPELMRRTRMG
                     RLDIATFGKQNGKGGKLLRSVGSWVGNHARRGKSKDKHTQYLKLVDRLPGILKFVPTA
                     GALTDAKNYLYLFCYFLQPTPANIQSMLLYSLTQYVRDERLKKIRVTKPEQMPSVAIY
                     HPNAKALFQSFAEYRKWYRKPLDPDATIGLLLMRPQVISKTTKHYDALINAIEAEGLS
                     VVPAIATLMDNREAVSKFFVENGNARVSQIVSLTGFSFVGGPAMNDSAAASEFLRELN
                     IPYRSAVSLDTQTIEAWSDSQTGLNPVQAGMQIAIPEIDGATEPFIYGGIPTRGQEPV
                     ALDDRCERLARRLKRWHTLQTAPRKDVKLAIVLFCFPPNKGNIGTAADLDVFPGVFDV
                     LKRLDTEGYQVELPDSAGDLRDRLNDNISYRMNVQEYRRLCPYVREVENDWGTAPGAI
                     NTFGKDLLIQGLELGNVYIGVQPTFGFEGDPMRLMMARGGSPHHGFMAFYTFLSKVFK
                     ADGVIHFGTHGAMEFMPGKQIGLSGECWPDRLIAELPNFYIYSVNNPSEGSIAKRRSY
                     AELISYLTPPIENAGLYKELAGLKDLVMAYRQSTNESERDQLYQSIEALRQQANF"
     gene            complement(11217..11483)
                     /locus_tag="VFH96_03305"
     CDS             complement(11217..11483)
                     /locus_tag="VFH96_03305"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET6973022.1"
                     /translation="MSKPHVIIYSRPGCHLCDDAKAAIMSAGCSDQFTLEEVNIESDE
                     ELLRKYKYDIPVIAIEGVESFIHRVNPKEFETRIKRIKSGFKAV"
     gene            11723..12331
                     /locus_tag="VFH96_03310"
     CDS             11723..12331
                     /locus_tag="VFH96_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6973023.1"
                     /translation="MDSQADQLSAPSVSGVSAATPRAAAAAAKPAASAAVVETSSDPP
                     VNKMRRRIVWATVAGSLITFFLMFVRFFLPRSILEPSSSFRIGPPADYAIGVDTKWQQ
                     QYRIWVTRTSDRLFVIYARCTHLGCTPDWKASENKFKCPCHGSGYDSEGINFEGPAPR
                     PMDRAKVSLDAEGQILVDTGKLFSWPKGETSHFDDKDAFISV"
     gene            12349..>13122
                     /locus_tag="VFH96_03315"
     CDS             12349..>13122
                     /locus_tag="VFH96_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET6973024.1"
                     /translation="MDTDPKDKKQLTAGEEDDNRSLIEKVKETGGSLKERAIEEAKEY
                     KDPQDTQLWKSIFRVSHDRSDPRNRSLAILSNVFLHLHPAKINRDAVRYPFTWGMGGI
                     TFYLFIVLTFTGVLLMFYYHPTKADAFRDILYLEHDVPFGKLLRNMHRWAAHLMVITT
                     WLHMFRVVLTGSYKRPREFNWCVGVVLLVLTLLLSFTGYLLPDDQLGFWAVTVGTNMA
                     RATPMVGHEGPFGPQLGLTAYNDVRFGLLGGSIVDSNALL"
BASE COUNT         2952 a         3573 c         3756 g         2841 t
ORIGIN      
        1 cgatgaagcg caccgtcaga ccgtcaggct cgcgtttcac tgtaccttcc tgaacgagcc
       61 cgccgatacg aaacgtgcga ccaaccggcg cttctttggc cgcgacttgt gtcgggctaa
      121 agaagaacac gagattattg cgaaaagcac tcgcggccag gtaggcagcg agcgacaaac
      181 ccgcaagccc gacaactata aaagctatcc gcttatgacg tcgtttcatt gatacttcct
      241 ctcagtaccg gcgtcgaggt gggtttggat gactgctgct gttgttgcgt taacgctttc
      301 tttctctgtt tcaggctgac gacttcccaa acgaaaaaga tgagtgaagc gatatacgat
      361 ccccaaacat agaagccgta gccgcccatc gataaatctg gccagttcat gattagactc
      421 ctacctccgc gaatcgcgca gcgttctgtt cgcgttcaag aataatgcat cgcagccgaa
      481 tcagcactac ggccacggag tagagccagc aagccgcgag catgatgagc aacgccgcca
      541 gcatcgatcc gcgaatcgtt ggtttctgaa gcaggcgaat cgtggcgccc tggtgcagcg
      601 tgttccacca ctgcacagag aagtaaatga tcggaacatt gatcacaccg accagcgcga
      661 gtacagcgcc cgcgcgatct gcgcgacgcg gatcatcgat cgctgcctgc agtgaaatga
      721 atccgatgta gaggaagagc aggatcaagg ttgacgtcat gcgtgcgtcc caaacccacc
      781 acgtgcccca cgtcggcttg ccccagaacg cccccgtgat cagcgatatc gctgtgaaca
      841 tcgcgcccgt gatcgcgagt gacgatgcca tcatcgatga gaggcgcgcg ttgaatgccc
      901 aaccaattcc cgcgtagatc gccatcagga cataaagaaa cattcccatc cacgctgccg
      961 gaacgtgaat aaagatgatc cggtatgcgt cgccctgctg gaagtctgtt ggtgcgatga
     1021 agaacccgac aaacaggccg gtagcgaaga gcagtgcagc gggaatcgcg aaccacggga
     1081 tcagcttccc cgcgagcggg tagaacattg ctggagatgc gtatttgtag agattcatga
     1141 tttttaagaa taggacctat cggacctata ggacctattt cctcactcca aagagatctt
     1201 tagcgcggct gcggttgccc agggcgaaaa caacagcgag acgatcgcaa atgcgccgag
     1261 cagcgacaga tgcgccgacg ggctcgtccc catcacgctc gcttcaaccg cacccgcgcc
     1321 gaagatcaac gccggaatgt aaagcggcaa cacgagtaac gagatcaaca cgccgccccc
     1381 acgcagtccg agtgtcaatg ccgctcccac cgatccgatc aaactcaaca ccggcgtgcc
     1441 caccagtaaa ctcacgaaca gaatccacaa cgtctcacga ctgagatcga attgcactcc
     1501 cacgagcggt gcaatcaaca cgagcggcag ttccgccacg atccagtggg cgagtatctt
     1561 tcccagcacg acgagaaaca gcggctgcgg cgtcagtaat aactgctcga gggtgccatc
     1621 ctgatagtca ttcgcaaaca gccgtccgag cgagagcatc gacgccagca acgctgccac
     1681 ccaaaccacg cccggcgcga tcgttcgcaa caactgcgtt tcgggtccga tgccgagcgg
     1741 aaaaaggctc acgacgatca cgaaaaagaa aaccgtcgac agcatgtcag cgcggcggcg
     1801 ccacgccagc atcagatcgc gccaaacgat ccagcgcaac atcgataagc tcgaaggtgc
     1861 gttcacgttg tgagttcgag tcgtttggtg tggcctgccg gcaactccag gtcctggtgt
     1921 gttgctacga tcgcaatacc gccacccgct acgtggtttt caatcaaaga tctaacaagc
     1981 gaaacggcgc ctttgtccaa tgaggtcagg acctcatcca acagccagag cttcgtgtca
     2041 caaacaagaa gacgcgcgag ccccgctctt cgtctctggc cttctgacaa cattcgtgcc
     2101 ggaagcgact cacgaccggc cagtcccatc tgtgcgagag cctctgccgc gcgttcttta
     2161 ctgaccgaaa caccattcaa tgcgttcgaa attctcaggt tttcaacggc gctcagctcg
     2221 tcttttatgc cgtgacgatg cccgaggtaa gtcacctgcg cgacatattc ctcacctaat
     2281 gagcgaatat ccgcatcgtt ccagctgatt ttgccttcag ccggagtgag aaggccgcac
     2341 aagatccgga gcaaactggt tttgccgctg ccgtttggtc cggtgagctg gatgaacgtt
     2401 cccggagaca ctgacagatc gagttcgcta aaaagcctgc gatctccgcg cacgcagccg
     2461 agcttactga cctctaatat tttgtcgtcc tccgccgcga ttgtaacgga ttgagggcgg
     2521 ttagtcagtt ttcgctcgcg gctcaacggg accgctcacg cgcagccatt cctgccaacc
     2581 gccttgcaaa accttcacgc gcttgaatcc ggcggcttgc aggatctgcg ccgcggcgat
     2641 actcgattga tctttagggc aagcgcaata agtgatgatc tctcgatcct ttgggagatc
     2701 tttgaagggc gggtaggtga gccgcgactt caactttcgc accttgaagt gaagcgcgcc
     2761 tttgatggtg gttgagctgg ccgcataacc ttcggagctg cgaacgtcga tgatcgtgac
     2821 cggatgattg ctcgtgactt ttgtcttgag ctcgtcagca ctaatcatcg cgatgtttga
     2881 cgcgtcctgc gcatgggcca cgatcgcagt cacgccgacg attatcaatg cgaggatcga
     2941 atgtttcaca gtgctcatta tcagcctcct tgtactcatt tcatcgtgcg tgacttgtga
     3001 aacgcaccgg gcgcgttttg agcgtcgtcg aacgtcttcg ggtcgtacca cgaataggta
     3061 gcctgcgcga aataaagata gccgggcgtg ccgccaaccg tttcgccgat cagatcgccc
     3121 tgtggtcgcg gtaacttcct gccgctgttt gcggtgacaa actcattcag actatgcact
     3181 ttcaaattct ggcccaccac gtctttcaga acgaacgttt gcgtggcgtc gggatcgcgc
     3241 cagtgattag cgccaaagcc gtgaatgacg gcgagaaacg tttcgtcttt ctcgatcggt
     3301 gcggcagctc gcgacgcttt gtggttgcgt ggttgtcgca cgagccaggg cggcaattcc
     3361 tggttcatca ggtcgagctt cgccggtttc aggatcactg cgagatcttg tgaaccgtcg
     3421 ccgttgaagt cgccggagag gaagcttgga ttgtaattcg tatcgatgac ggcagcgtct
     3481 ttgaagacgc gcttgacggc ctcctggacg tcggattgtt tcgggcctgc gaccgaagtt
     3541 aattgtactg gcggtgtcga ttgaggactg ggctggtaag cgactggcgg cgcttccacc
     3601 gccggacgct ttgctgagct acatgcgcaa aacgttaacg cgataaggag gataacaatt
     3661 ctcatcatca tgaccctctt gttacttcca cagattacac agattacaca gactgtaatc
     3721 aatctgcgtg atctgtgtaa tctgtggata aacgaagccc gccggacatg atccggcgag
     3781 cctcgggata gtgcctcgtc agttcggctg ccgccagtag atcataacga caacacgaaa
     3841 gcaatcaggt cgttccgttg cgcagcggtc agggcattga agttattcct ggccgtcgtt
     3901 gcctgtccgg cgtgcagttg gaccgagtca aatatcgtca cgttcaggcc ttcatgcatg
     3961 agccggttac gtgcgcgaat gccccatagc ggcggcgttc gaatttgatt ggccgatccc
     4021 tgtcccccgt tttgcacaat gccatcaccc gtgccgatgt tgtgtagggc aaaatcgctg
     4081 aacggatgga tgatcttgtt gccgagagct gccggaaccg tgaacgcgcc accattgatg
     4141 agcgttcccg gagctgccgt aacgaaagtg cgcgtgtggc aaacattgca accaaccgcg
     4201 ttgaagagcg aatccccacg cgtcgccgcc gccggaatcg ggttctgacg accgggagca
     4261 cgcgttgccg ccatgaaatc ggcgaacgcc aacacatcgt caccgtcgtc ttcaggatcc
     4321 ggaaccggat cgaaggctga cgggaacgtc acgttgatca caccctcagc cgtgctggcc
     4381 gggttttcgt gtgtgccggc gtctgcggcg tcggaacttc tgccgccgaa accgtcaaac
     4441 gggttggtga tgcccatttc gttcaggtag gcatcgcccg agaacgaaag caggctcgcc
     4501 tgttgagcct tccagccgaa ccggccgata cgaagcgcgt taccagcttc ggttacggga
     4561 actgttgtga gagttccacg ttgtgcgacc ggctgggcgg ccacgttatc ttgcagtgtt
     4621 tggttggcaa gagcctctac gtagccatcg cccagtgtgc ttagcgtgac gcggaaagct
     4681 ttgacggtct cagccgtgga gatgtgttcc actacgtctg aatccgtccc gcgagcatga
     4741 atcaattgcc cgccgggtgc gtcaacgaaa gcaccgttag taatgtgacc ggccctgaat
     4801 tccatttgct ggccgaaggc gccgacgtca actgactggt ggcactcaac gcaaccgacg
     4861 tcgttgtacg ttggtccaag gccatcttcg atggtctcgc gttccgagaa gatgaatttg
     4921 ttgtcttcga atctgccatt cccgcgcgcg gtgggtgcta caggttcatt gacgcattcg
     4981 acgatcgggg tgcccttaac gccgaagccg ttgaacaggt catcggtttt ggcatccatg
     5041 tcggtggtca gtgcctcggt tgcagcttga ccaatgacag gctggggctc gctcaggggt
     5101 acgacgagcg cgaccgcgaa tagaactaga gtaaggacag gtagaacttt cctcatttga
     5161 ctttgacctc ctaggagcta ctaacgggtt gcgacacaaa acctacagct tgcccgtcgg
     5221 caaactgcac agttctgctc tagttttggt gaaaaaaccc tgcttggact tgatatagag
     5281 gcagctcaag tcttaggggg ttcgaccaga gttatgagtc ccctcacgaa acaagcaacc
     5341 aaggcttagc cctagttgtg agagagagag tagaggcgcg gtctctgctc gaaaagtaaa
     5401 tcgaatttag ctgggcagta ctttgaaagt cgggagggcc gcactctatt ccggtttagg
     5461 agcaaagtca agaaaattgt atccgcagat tacacagata tttgaaatca ggtaatctgt
     5521 gtaacctgtg ggtagactaa accgaaaaac ttatccgttt acggcgatcg tcgggcagga
     5581 ggaaatgaag ctcgccctgc tgctgaacgt cgtcgacccg ctcatcggcg gtgtcttgat
     5641 catgggacat cgcggcacag gaaagtcgac tgcggtgcgc gcgctcgcag acttgttgcc
     5701 gcagatcgac gtcgttgccg gttgtcctta taactgtgat ccgaaagacg atctgtgcga
     5761 tcagtgtcgt ggctcagaga tcacttcgaa aaagatgctg gtgcccgtgg ttgagcttcc
     5821 actcggcgcg actgaggatc gtgtttgtgg gaccatcgat atcgagcgcg cgctgtcggc
     5881 cggtcgcaaa gctttcgatc ccggactgct ggcacgcgcg aatcgcggtt ttctctatat
     5941 cgatgaagta aacctgctcg aagatcatct cgtcgatctt ttactcgatg tggctgttac
     6001 cggcattaat aaggtcgagc gtgaaggtgt ttcggtcgaa catcccgcga gctttgtgct
     6061 gatcggatcg ggaaatcctg aagaaggcga gcttagacca cagcttctcg atcgtttcgg
     6121 attgcacgct gaagtcacga ctgaaaacta tttacagaac cgcatcgata ttatcgagcg
     6181 tcgtgacggt tacgatcgag atcgtgaggc tttctgtaag tcttacgaag cggaccagga
     6241 gcaactgcgc aaacgcatca cacgcgcacg cgcgaatctc acgaagatca cagtcgaacg
     6301 gccggtgttg gagaggatcg cgcagctttg tgctgatttg aaggtcgatg gtcatcgcgg
     6361 tgaactgaca atcatgcgcg ccgcacgtgc gctggcggcg tttgaaggcc gtcgcgcggt
     6421 tacggacgat cacgtgaagc gtgtgtcagc gatggcgttg cgacatcgtt tgcgtcggga
     6481 tgctttagat gagaccgcga cgagcgaaca gatccagcag gccgttgatg aagtgtttcc
     6541 gaacccgccg cagccctcac aatcgcctgg gaatggcggc ggcgatactc aaaatctcga
     6601 tcgacccggc aaagtaaaca aagatgctcc gcgacagcgt cgttctgcat ccggggcaag
     6661 ctcgcgaccg aacgatgccg atgtgttatc gccacctgcc gtcgagcgca aatcgggcga
     6721 agtgaagctc gaggaacaga tgcgatcgac cgatcgagct gataagtcgc gcttgttgag
     6781 ccggcgcgcg agtggaggaa aggcggcgct ggtccaacaa cgcggccggt acacgcgtgc
     6841 ggtcacgttt aaaagctcag gcgctcgtat cgcgctcgac gcgaccctgc gcgcgctcgt
     6901 gagtttggag aagcgtgctt tgcctgtgag cacgcaagca ctgcgctaca agctcctcaa
     6961 acacaagcgg ggcaccttgt ttgtctttgc gatcgatgcg tcgggaagca tggcggcgaa
     7021 tcgtattgcg cgggcgaaga gcacgatcct caagttgctg cggaagtcgt acctgaatcg
     7081 cgacagcgtg gcgatcgttt cgtttcatgg cacaacagcg aatgttgatc tgcctccgtc
     7141 gcggagtatt ctgcgcgcgc ggcgcgtgct cgactcgttg cgaatgggtg gcagcacgcc
     7201 actcggacta gggctcgtga cgacgatcga gttgctggag ttagtgggca acaaatttgg
     7261 tgagacggtg gtgttgctgt ttactgacgg ccgttcgaac gtgccgctca ggcgcggtgg
     7321 tcttaacctg cgcgcgttcc ggcaagttaa gatcgagtcg gagttgagag agctgaccgt
     7381 cgcgctgcag cgaacgaaag cgcgagtggt ggtggtcgac acgcaaagag aattcgaatc
     7441 cgctgaagaa acgcggcgtc tggcaagtat tctgaacgcg cggtttgtaa aaatcgctcc
     7501 ggccaatccg tgaaatccgt ggcaaaaagt gcaaacgaag agcgcgacgc aaaagtcttc
     7561 gcgctttacg agcaactcgt tgagatcgag cagcggttga taccgaccgg tctgcacgtt
     7621 tttggccgcc cttcatcagc gggtgagaaa acggatctgc tcaagatgat cgcgtcgttc
     7681 gatcgtcctg aaacgggcgc gcgtgccata ccggaagaat tgctcgacga ttctgtgcga
     7741 gagttcaccg cgaatggcgt cgctgccgcg gtgagctttc tcgagcagaa ggggttgagc
     7801 gccgcagcca cacgacctgt tttcacgctg ctggaatcag tctcgacaaa actcaaaacg
     7861 aataacgaga tcgattcact cctgcgtgct ctgcgtggtg aatttatcga gcctggtccc
     7921 ggcgccgata tcattcaaaa cccggacatc cttccgaccg ggagaaacac gcacgcgatc
     7981 aatccgtacg gtgtgccatc ggccgccgcg tttgcgcgca gtgagaacgt cgcaagcagc
     8041 ctcctcgagc gatataaaaa cgagaacggc cgctatccgg aagcaatggc gctcgtgctc
     8101 tggggactcg acaacatcaa gacgcagggc gaaggcgtgg cccaggcgct ctggctgctg
     8161 ggcactcgtc cagttcgcga tgcacttaat agggccacga gcgtcgaagc gatcccactt
     8221 gccgagctcg gccgtccgcg catcgacgtc gtgatgacgg tctccggcat ctttcgcgat
     8281 ctgtttagtc cgactgtgca actactcgac aaggcagtgc gactggtggc ccaactcgac
     8341 gagccgctcg agctcaacta cgtccgcaag cacgtcctgc agcaggtgga agaaacgccc
     8401 tgtgatctcg atgaagctgc cgtacgcgtg ttttcaaatg ccccgggaaa ctatggcaca
     8461 aacgtgaact tcatggtgat ggattcgcag tgggaccgcg aggagacgct cggcgatctc
     8521 ttcgtcacgc gcaagtgttt tgcgtacggg cgcgactcaa agggccgtgc gtttgaggga
     8581 agggaagcac gcgtagcaat ggaccgcgcc ctctcgcgtg tcgaggcgac gtatcagaac
     8641 atcgacacgt tcgagatcgg tttgaccgat gtggaccact attttgagta tctcggtggg
     8701 gtgtcgaagg cggtggagac gcgggcgaag gcgcggcccg cgatctatct gtcagatagc
     8761 ttgtcgcgtg aagtgaagat tcgttctctg gaagagacgg tgcgtttgga aacgcgggcc
     8821 aagacgctca atccgaagtg gtatgaggga atgttgaagc atgggtttcg cggagtggct
     8881 gagatcgaga accatgtgtc aaacacgttt ggatggagcg cgacggcgga tgcagttgat
     8941 gattgggttt acacggaagt ggcaaatacg tttttgtttg atgagcagat gctggagcgt
     9001 ttacgcgatc tgaatccaca ctccgcgcac tcgcttgcac aaagattgtt ggaggcgaac
     9061 gggcgcgggt actgggacgt tgatgcgagc gtgttggata aattgcggga gattagcagg
     9121 gacctcgagg atcaattaga aggggttgct tagccacaga ttacacggat tgaaaatcac
     9181 gattttatat gtcggctcga gtttattggc gccgctcaag caggcggagc gggagattaa
     9241 tcgtgcctac aagctcgatc tgagtgtcgc ggcgtacaac ttcgggtcgc cgctgagcga
     9301 tagtgagtgg gtcgaggttg aagaggattt aaagaaatca aatatcgttt ttgtcattca
     9361 cgtaatggat ggcgagaatg cgacgcgtct cgtttcggca ctcgataaag tgagaaacga
     9421 tgcggttgta gttatcaact gcatgcctga actcatgcgc cggacgcgta tgggccgcct
     9481 cgatatcgca acgttcggca aacaaaacgg caaaggtgga aagctgctgc gctcggtcgg
     9541 ctcctgggtc ggcaaccacg cgcggcgcgg taagtctaaa gacaaacaca cgcagtattt
     9601 gaaactcgtc gatcgcttgc cgggaattct caagtttgta ccgactgcgg gcgcattaac
     9661 cgacgcgaag aattacctgt atctcttctg ttacttcctt cagccgacac ccgcgaacat
     9721 ccagtcgatg ctgctctact cgctcacgca gtacgttcgc gacgagcgac tgaagaagat
     9781 tcgcgtaaca aaacccgagc agatgccttc ggtagcaatc tatcacccga acgcaaaagc
     9841 cctctttcaa tcgttcgctg aatatcggaa gtggtatcgc aagccgctcg atcccgatgc
     9901 gacgatcggc ttgctgctga tgcgtccgca agtgatcagc aaaacgacga aacactatga
     9961 cgcattgatc aacgcgatcg aagcagaagg cttgagcgtg gtccccgcga tcgcgacgct
    10021 catggacaat cgcgaagcag tctcgaagtt tttcgtcgag aacggaaacg cacgcgtcag
    10081 ccaaatcgtt tcgttaaccg gcttcagttt tgtcggcggg ccggcgatga acgacagcgc
    10141 tgcggcgtcg gagtttttgc gcgagctgaa tattccttac cgctcggccg tgagcctcga
    10201 tacacaaacg atcgaagcat ggtccgattc acagaccggc ttgaatccgg tccaggcagg
    10261 catgcagatc gcgattcccg agatcgacgg tgcgacagag ccgttcatct acggtggcat
    10321 tcccacgcgc ggtcaggagc ccgtcgccct cgatgatcgt tgcgagcgat tagcacgacg
    10381 cctgaagcgc tggcacacat tacagaccgc acctcgcaaa gacgtgaagc tcgcaatcgt
    10441 gttgttctgt tttccgccaa acaagggaaa catcggcaca gccgctgacc tcgacgtgtt
    10501 tccgggtgtg ttcgacgtgc tcaaacgtct tgacacggaa ggttatcaag tcgagcttcc
    10561 ggattccgcc ggcgatctgc gcgacagact caacgacaac atctcgtatc gcatgaacgt
    10621 tcaggagtac cggcggcttt gtccgtatgt gagggaagtc gagaacgact ggggcacagc
    10681 gccgggcgca atcaatacct tcggtaagga cttactaatc cagggcctcg agttagggaa
    10741 cgtctatatc ggagtgcaac cgacgtttgg ttttgaaggc gatccgatgc ggctgatgat
    10801 ggcgcgtggc ggctcaccgc atcacggctt catggccttt tatactttcc tgtcaaaggt
    10861 cttcaaggcc gatggcgtga ttcacttcgg gacgcacggc gctatggagt tcatgccggg
    10921 caagcagatt ggactgtcag gcgagtgctg gccggaccgg ttgatagccg agcttccgaa
    10981 tttttacatc tacagcgtca acaacccctc cgaaggatcg atcgccaagc gccgctccta
    11041 cgccgagctg atctcgtatc tcactccgcc aatagagaac gccggattgt acaaagaact
    11101 cgccggccta aaagatctcg tgatggcgta tcgccaatca acaaacgaat cagagcgcga
    11161 ccagctatat caatccatcg aagctctccg ccaacaagca aatttctaaa cacggattac
    11221 acggctttaa atccggattt tatccgttta atccgcgttt caaattcttt cgggttaacg
    11281 cggtgtatga aggattcaac gccctcgatc gcgatcacgg gaatgtcgta cttatatttt
    11341 ctgagcagct cctcatcaga ttcaatattc acctcttcaa gggtgaactg atcgctgcag
    11401 ccggcgctca tgatcgcggc ctttgcgtca tcacaaagat ggcatccggg acgcgaataa
    11461 atgattacat gtggttttga catcgatact tcttgccaga agttcaggaa attatgtata
    11521 agtagcgtcg ccgaccatat tatatttgtt ttttgttagg tgcggcgctc ttcatgttta
    11581 agctaataat tcgttttact gtgcagagag gaatccctgt aggcgaagtt gtataagtag
    11641 ccctctgtta gtaaagtcga agtgtatccg cggaagttcg cacacctggc aattcggaat
    11701 tgattttgac ggaggtctgg acttggatag tcaagccgat caattgtctg cccccagtgt
    11761 aagtggcgta agtgcggcaa caccgcgcgc cgcagcggcg gcggcaaaac ctgcggcttc
    11821 tgctgccgtg gtggaaacaa gctctgatcc tcctgtcaac aagatgcgcc gtcgcatcgt
    11881 gtgggccacc gtcgccggct cactaataac gttcttcctg atgttcgtgc ggttcttcct
    11941 gccgcgctcg attctcgaac cttccagcag ctttcgtatc gggccgcccg ccgattacgc
    12001 tatcggtgtc gacacaaaat ggcagcaaca ataccgcatc tgggtaacga gaacgtctga
    12061 ccggctcttc gtcatttatg cgcgctgcac ccacctcggc tgcacgcccg actggaaggc
    12121 cagtgaaaac aaattcaaat gcccgtgcca tggcagtggc tacgacagcg agggcatcaa
    12181 cttcgaagga cccgcgccgc gaccaatgga tcgcgcaaag gtctcgctcg atgccgaagg
    12241 gcaaatcctg gtcgataccg gcaagctctt ctcgtggcca aaaggtgaaa caagccactt
    12301 tgacgataaa gacgctttca tcagcgtata gcgcaactgg gaacgtggat ggatactgat
    12361 ccaaaagaca aaaaacaact gaccgcgggc gaagaagacg acaatcgcag cctgatcgag
    12421 aaggtcaagg aaaccggcgg ttcgctgaaa gagcgcgcga tcgaagaggc gaaggagtac
    12481 aaggatccgc aggacacgca gctctggaag tcgatcttcc gcgtctcgca cgatcgttcc
    12541 gacccgcgca accgctcgct cgcgattctt tcaaacgtct ttctgcacct gcatccggcg
    12601 aagatcaatc gcgacgcggt gcgctaccca ttcacgtggg gcatgggtgg catcacgttc
    12661 tatctcttta tcgtgttgac gtttaccggg gtgttgttga tgttttacta ccaccctacg
    12721 aaggccgatg cgttccgcga cattctctat ctcgagcacg acgtgccgtt cgggaagtta
    12781 ctacgcaaca tgcatcgctg ggccgcgcac ctgatggtga tcacgacctg gctgcatatg
    12841 tttcgcgtcg tgttgacggg ttcttacaag cggccacgcg agtttaactg gtgcgtgggc
    12901 gtggtgctgc tcgtgttgac gttgctgttg tccttcacgg gttacctgct gccggacgat
    12961 cagcttggat tctgggcggt aacggtgggt acaaacatgg cgcgcgcgac gccgatggtc
    13021 ggtcacgaag gtccgttcgg gccgcagtta ggactgactg catataacga cgtgcgcttc
    13081 ggactgctcg gcggatctat cgtcgactcg aatgcgctgt tg
//
LOCUS       DASSFQ010000102         4565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_40784, whole genome shotgun sequence.
ACCESSION   DASSFQ010000102 DASSFQ010000000
VERSION     DASSFQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40784"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..675
                     /gene="ribA"
                     /locus_tag="VFH96_03320"
     CDS             52..675
                     /gene="ribA"
                     /locus_tag="VFH96_03320"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET6973025.1"
                     /translation="MTLRLIHSACQTVERVAEARLPTETGEFRIIGFRSLDSEEEFVA
                     LVKGDLRSDRSSLVRIHSQCMTGDVFGSVKCDCGRQLQAAMKQIEQEGHGAIVYQQQE
                     GRGIGIINKIRAYALQDEGADTIEANERLGFAADMRRYEQCAEILLDLGLRAVRVMSD
                     NPAKIQALRQAGIKVVERVPLEVEPHDAFVNYLKTKKEKMGHLVSVA"
     gene            684..2285
                     /locus_tag="VFH96_03325"
     CDS             684..2285
                     /locus_tag="VFH96_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6973026.1"
                     /translation="MSLKEVHLANTLDLHDGEMKEISVAEVKILLARVGDNFHAVSAT
                     CPHYGGPLAEGTLCGARVMCPWHHAAFNVVNGNLEDPPALDSLVSYPLRIDGERIMLS
                     LPEDTADRRMPAMSKRDTSVDSRQFVIIGAGAAGYAAAQTLREEGFRGSVVMITREDR
                     APYDRPNLSKDYLHGHADPEWMPLRGEDFFKEHDITLLLNREVTSVDARNRTITFESG
                     ETMYYDALLVATGGAPVRLNIHGSDLKNVCVLRSFADADSIIETAKHCPRAVVVGASF
                     IGMEAAYSLRERGLEVTVIAPSQEPFEATLGAEVGALFRRVHESHGVRFKLGNIVYGF
                     VGNHTVEAVALDNGELIETDMVVVGVGVRPVTQFLEGVELDQGGAVVVDSRLRAADGL
                     FAAGDIASFLDARTGEYVRIEHWRTAQQQGRTAARNMIGNNFTFDAVPFFWTRQFDMG
                     LLYVGHAAGWDEIIYRGDVAAQDFLAFYVKDNRVLAVAGMNRDREMAAAEELLRLDRM
                     PTREQLQLNERSLVEMLQMPELISA"
     gene            2369..3082
                     /locus_tag="VFH96_03330"
     CDS             2369..3082
                     /locus_tag="VFH96_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6973027.1"
                     /translation="MGVAASPIVKFPGPRHNRRVQLFSGLESDEMSRVVAHARSLRKT
                     RGEFIYMPGDRADFVYILKHGRVKLSVLSESGKEIAIDIIQSGEIFGEFALVDESQRS
                     NMTQALDDVLMWVFSKHDFTKLLTSLPMLSLSYIKLVGDRRRRMEKKLSDITSKPVSG
                     RICELLHELATGSAEFESAAPDYIVPLTHQDVASLIGAARQTTTTVLNDLERRSIIEL
                     GRGSIRVKRLKELQTYAAF"
     gene            3291..4190
                     /locus_tag="VFH96_03335"
     CDS             3291..4190
                     /locus_tag="VFH96_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973028.1"
                     /translation="MKNRKTSNWTIWLAVVALALTMVASLNFSTSVSADGGSDLGTSP
                     FDFSDATYRAHGIIPENIVLRVGTPSRTGDFVVDNTNTDPNRANVRTIETTPGTTGSS
                     GLTYANIFGVLNSNSFEHDAAGNLTARAQASFDTAEHFRVFIFPKASNGSILDPFLPN
                     KRQDNMFDTRDGYFSNDPLGMWVAVWVVYTPKAFTKEGHKELDPIAATNGRDLDGTAI
                     LTSPSQIDNLASKGFIELRTRPVNDGVFRWVLCPIPEDPRNGFIRPDAFIRDVKRADG
                     TPVFPALGNNFRCLQQTGDWCHN"
     gene            4303..>4565
                     /locus_tag="VFH96_03340"
     CDS             4303..>4565
                     /locus_tag="VFH96_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6973029.1"
                     /translation="MSDQKQFAGRVAIVTGGTRGIGRAIALELGKRGADIAFNYAKSA
                     EAAESLKSELEALGVRSLAVQCDVASTDAAAAMVQQVKEAFGR"
BASE COUNT         1013 a         1260 c         1294 g          998 t
ORIGIN      
        1 ccacggaggg ccacccctac agttgatgca agtgaggaga ccaacaaaaa gatgacactc
       61 agactaattc actccgcgtg ccagacagtc gagcgagtgg ctgaagcgcg actacccacc
      121 gagacgggtg agtttcgcat catcgggttc cgctcgctcg actccgagga agagtttgtt
      181 gcgcttgtaa aaggtgacct gagatctgat cgttcgagcc tcgtgcgcat ccactcgcag
      241 tgcatgacag gcgatgtctt tggctcggtc aaatgtgact gcggtcgaca acttcaggcc
      301 gccatgaaac agatcgagca ggaaggacac ggcgccatcg tctaccagca acaggaagga
      361 cgcggcatcg gcatcatcaa caagattcgc gcgtacgcac tgcaggacga aggcgctgac
      421 actatcgaag ccaacgagcg gctcggcttc gcggcagaca tgcgccgtta cgagcagtgc
      481 gctgaaatcc tgctcgatct cggtttgcgc gcggtgcgtg tgatgtctga caaccctgcg
      541 aagatccagg cgctgcgaca agccggaatc aaagtcgtcg agcgcgtgcc tctcgaagtg
      601 gagccgcacg atgcattcgt caattacctc aaaaccaaga aggagaagat gggccatctc
      661 gtcagtgtgg cctaactact gcaatgtctt tgaaagaagt tcatctcgcg aatactctcg
      721 atcttcacga cggcgagatg aaagaaatct cagtcgctga agtaaagata ctgctggcgc
      781 gtgttggtga taactttcac gcagtcagcg cgacctgtcc tcactacggc ggaccactcg
      841 ctgaagggac gctgtgcggc gctcgtgtta tgtgcccgtg gcatcacgcg gccttcaacg
      901 tcgtaaacgg caatctggag gatccacccg cgctcgactc gctcgtttct taccctctac
      961 gcatcgatgg cgaacgcatc atgctgagtc tgccggaaga cacagcagat cgacgcatgc
     1021 ctgcgatgag taagcgcgac acgtccgtcg actcgcgaca gttcgtgatc atcggtgcgg
     1081 gcgcggccgg ttacgcggcg gcgcagacgt tacgcgaaga aggtttccgc ggcagcgtag
     1141 tgatgatcac gcgcgaagac cgcgcgcctt acgatcgacc caatctcagc aaagattatc
     1201 tgcacggcca tgccgatccg gagtggatgc cgctgcgtgg tgaagacttc ttcaaagagc
     1261 acgacatcac gctactgctg aatcgtgagg ttaccagtgt tgatgcacgc aacaggacga
     1321 tcacgtttga aagcggcgag acgatgtact acgacgcgct actcgtcgct accggcggtg
     1381 ctcccgtgcg cctcaacatt cacggctcgg atctgaagaa cgtttgcgtg ttacgcagct
     1441 tcgccgatgc cgactcgatc atcgagacgg ccaagcactg tcctcgcgcg gtcgttgttg
     1501 gggcgagctt catcggcatg gaagcggctt atagcctgcg tgagcgcggt cttgaagtaa
     1561 ctgtgattgc gccgtcgcag gaaccgtttg aagccacact cggggcggaa gtcggcgcgt
     1621 tgttccggcg tgtgcatgaa agtcacggcg ttcgtttcaa gctcggcaat atcgtctacg
     1681 gctttgtagg caatcacaca gtcgaagcag tcgcgctcga caatggcgag ctcatcgaga
     1741 cggacatggt tgtggtcgga gtcggagtaa gaccagtcac gcagtttctt gagggagtgg
     1801 agttggacca ggggggagca gttgttgttg attcgcggct acgtgccgct gacggacttt
     1861 ttgcggcggg tgacatcgcc agctttctcg atgcgcgcac gggggagtat gtgcggatcg
     1921 agcactggcg cactgcgcag cagcaaggac gtacggcggc acgtaatatg attggaaaca
     1981 atttcacgtt cgatgcggtc cctttcttct ggacgcgaca gttcgacatg ggtctgcttt
     2041 acgtcggaca tgcggccggc tgggatgaga tcatctaccg tggcgacgtc gcagcgcagg
     2101 actttcttgc tttctatgtg aaggacaatc gcgtgctcgc ggtcgccggc atgaatcgcg
     2161 atcgcgagat ggcagcagca gaggagttgt tgcgtctcga tcgcatgcct acgcgcgagc
     2221 agcttcagtt gaatgaaagg agtctggtgg aaatgcttca gatgcccgag ttaataagcg
     2281 cctgagaagt cgtcaggatg caagctacct cactccatgg agttcgccca gtgctccaaa
     2341 aacaacctcc cgttaggagt tacgtttcat gggagtcgca gcatccccga tagttaagtt
     2401 tccgggtcct cgacataacc ggcgagtgca actcttctcc ggtctggaat cagacgagat
     2461 gagtcgcgtc gtggcacacg cccgcagtct gcgtaagacg cgcggtgagt ttatctacat
     2521 gccgggcgat cgcgctgact tcgtttacat tttgaaacac ggcagagtga agctttccgt
     2581 gctttcagaa tcaggcaaag agatcgcgat cgacatcatt cagtccggcg agatctttgg
     2641 cgagtttgcg ctcgtcgacg agtcgcagcg cagcaacatg acccaggcgc tcgacgacgt
     2701 gttgatgtgg gttttctcga aacacgattt cacgaaactc ctaacgtctt tgccgatgct
     2761 gtctttgagt tacatcaagc tcgtcggcga tcgcagacga cgaatggaga agaagctttc
     2821 tgacatcacg tcgaaaccgg tgtcgggccg catctgcgag ctgttgcacg agctagcaac
     2881 gggctctgct gaattcgaaa gtgctgcccc tgattacatc gtgccgctca ctcaccagga
     2941 cgttgcgagt ctcatcggcg ccgcgcgtca aactactacc accgtactca acgatctcga
     3001 acgccgcagc atcatcgagt tgggccgcgg ttcgattcgt gtcaagcgcc tcaaagagct
     3061 tcagacgtac gccgcttttt aggaagtaca caaaaggcac aaaaagcaca aaatggttac
     3121 ttagtttcga cggcgctcgc tccgcctgga tgcagatttt attttgtgct ttttgtgcct
     3181 tttgtgtact tgcttttttc ttgcactgtc gccgcgctga cattccttct aacactcttc
     3241 atacatgatg cgcacctcgt ttgagagaca tatgaaggag ctcaccgatc atgaaaaata
     3301 gaaaaacttc aaattggacc atctggctcg ctgtcgtcgc actcgcgctg acgatggtag
     3361 ccagtcttaa cttcagcacg agcgtttccg cggatggcgg cagcgatctc ggcacatcac
     3421 cattcgactt cagcgatgcc acctaccgcg cccacggcat cattcccgaa aacatcgtgc
     3481 tgcgtgtcgg cactccgtca cgcaccggcg acttcgtcgt cgataacacc aacaccgacc
     3541 cgaaccgcgc caatgttcga actattgaaa ccacaccggg aactaccggc tcgagtggtc
     3601 taacttacgc gaacatcttc ggcgtgctca attcgaacag cttcgagcac gacgccgcgg
     3661 ggaatctgac tgcgcgcgcc caggcctcgt tcgacactgc cgaacacttt cgcgtgttca
     3721 tctttccgaa agcgtcgaac ggctccatcc tcgatccgtt tcttccgaac aagcgacagg
     3781 acaacatgtt cgacacgcgc gatggttact tctcaaacga tccgctcggc atgtgggtcg
     3841 ccgtttgggt cgtctacact ccgaaagctt tcactaaaga gggacacaaa gaactcgatc
     3901 cgatcgcagc gacgaacggt cgtgacctcg acggcacggc gatcctgacg agcccgtcgc
     3961 agatcgataa tctcgcctcc aaaggtttta tcgagctacg cacgcgtccg gttaacgatg
     4021 gagtgtttcg ttgggttctc tgcccgatcc ccgaggatcc ccgcaacggt ttcattcgtc
     4081 cggatgcttt cattcgtgac gtgaagcgag ctgatggcac gcctgttttc ccggcgctcg
     4141 gcaacaactt ccgttgtttg cagcagaccg gcgactggtg ccacaactaa accattaata
     4201 gccacaaaaa ggcacaaaag gcacataaaa agtcggtcaa aacttttatg tgccttttgt
     4261 gcctttttgt ggctaaagct cttatgtgtt atttagtggc tcatgtcgga tcaaaaacaa
     4321 tttgcaggtc gcgtggcgat cgttaccgga gggacgcgcg gcattgggcg agccatcgcg
     4381 ctcgaattag gaaaacgcgg cgctgatatc gctttcaatt atgcgaagag cgctgaagcg
     4441 gcggagagtt tgaagtcgga gctcgaagcg ttgggcgtgc gcagtttggc agtgcagtgt
     4501 gatgtggcaa gcactgacgc cgcggccgcg atggtccaac aagtgaaaga agcttttgga
     4561 cgcat
//
LOCUS       DASSFQ010000103        12887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_492309, whole genome shotgun sequence.
ACCESSION   DASSFQ010000103 DASSFQ010000000
VERSION     DASSFQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492309"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1073)
                     /locus_tag="VFH96_03345"
     CDS             complement(93..1073)
                     /locus_tag="VFH96_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET6973030.1"
                     /translation="MDALGGSKWLWIGFSLFIFTMLSLDLGLFNRKAHTIKYREAWLW
                     SGVWVTLAMIFAGIVFYYLGTQPGFEFLTGYIIELSLSVDNLFVFLLIFSYFKVPAKF
                     QHRVLFWGVMGALFMRLTMIFVGAALINRFHWIIYIFGAFLVYTGIKMFRQDEIDVQP
                     EKNPLVRLVTRFLPISRHYEEEKFFTKANGKRTGTLLLLVLMVVEVTDLVFAVDSIPA
                     IFAVTTNTFIVYTSNVFAILGLRSMYFLLAGVVEKFRYLRFGLAIVLTFIGLKMLVGA
                     IGFHIPIKISLVFVALVLIGSVVASLVVSPEGERTITVDLPPDYDLPPDK"
     gene            1277..3292
                     /locus_tag="VFH96_03350"
     CDS             1277..3292
                     /locus_tag="VFH96_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6973031.1"
                     /translation="MLKQLAKWERTSKYIVIIFAALMAISLVIFYAPGRNSGNNIDPT
                     RNTEVVAKVGSASITVADVARIRENYAQMFGNRINLAQLGGNKRFLDGLISKQVISQE
                     AARLGLSASDGELRERIRKQFSDAAGNFVGFDRYKESVTARYGDIEKYENDIRDEIAQ
                     EKLRAFVSASVNVSDAEVEEEYKRRNTTFDVNYVALTSDKLATKIQPSDDELRAYYDS
                     HKTDYRYLEPQRKIRYVFVDTEKVGSKLQIPDTDLKAEYDKLDPEHKEAGIEIQQILL
                     KVARKDLDAQVEQKAKDLITKLRGLTGQATKEQFAEVAKGNSEDPATARNGGMLEKPF
                     KKNENKVNGLYDRTVDMRPGDVSDIPIRYAGNWYILRRGESVPKTFEQAKPDLLVSLR
                     NRKAYGQAFQLAQQAQTRLQETKDPQKVAQELAAKANMTPAEMVRETPFVKPGDDVPQ
                     IGSNQQFEQAIETLNNPNDVGNVTGVKGGFAIPMLAEKKDPRIPDFDEVKTKVAEEIK
                     KQRAKEQLEQKAKDLVASLTSADGIKAAGEKEGYEADTEESFKLGQSLGKAGTSAALD
                     DLIYSLKPGEFSKTPIKVDDKWVIVGVTKRNDADMNALAAQRDTLKSSMMSERQEQVF
                     DDYVAGVEQRMKKDGKIEIYPKVLAQLEEDEPAAEPGLPAGLNFPTK"
     gene            complement(3325..4329)
                     /locus_tag="VFH96_03355"
     CDS             complement(3325..4329)
                     /locus_tag="VFH96_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973032.1"
                     /translation="MKTRLLTVAIILVALFQLEAAAQTRSRNNAPAAPTLMSSLPQSD
                     AVAQVKVKQLLSEAMPRILAGNPAKLNEVNASLDRFKDRTGLDPRMFQQVALGIRFTY
                     PSEGVTKVQTVALAEGTFSAAAMVAAGRVASNGKYREEKYQDKTIYIFSIDEHVKLLG
                     LFDVRIGELAATPLDTNTLALGDPTGVRSVIDASRSRKRANAELIALANRDPNAVIGF
                     GSNITEQLVANLDIGNAPIGADLKSLRQVYGSVATTETDLQLFLAARAVNAEAAKNFG
                     DTLEGLKQFGALFVGRLSGAKGVLAKSALSNMKISSAANELQIRTSVAQAEVGPLLGN
                     "
     gene            complement(4504..5220)
                     /locus_tag="VFH96_03360"
     CDS             complement(4504..5220)
                     /locus_tag="VFH96_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973033.1"
                     /translation="MIVLRFVVVCVVLLVIVGCRSQTDTRTNATASPDTTVSSTPPFQ
                     TKEPEHYSAVRTITASADGRTVVTKAFIARDGESSRYESTGTLKNTIYLATPEGRFFL
                     LPDDKVYADVTDESKIADAQDDEELKSSPDALLHTDAGATSYQKLGNEVIAGRNTNKY
                     RIVVNSPAPANVSQSETLMWIDEALQMPIRSETKSSGTHVTMEISEIKLEVDKSLFAI
                     PHDYRKVTFTELRKRLAVND"
     gene            complement(5217..6398)
                     /locus_tag="VFH96_03365"
     CDS             complement(5217..6398)
                     /locus_tag="VFH96_03365"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET6973034.1"
                     /translation="MKNIYLAGAVRTPIGKFGGTLASWTAADLGVAVAKESLRRAGVA
                     ADQITDSIWGCARQAGGGPNVARQIIFRAGVPQTTPAATINQACGSGLKAIMLAAQEI
                     MLGRADAVLAGGTESMSRVPYFAEGARWGARLGNMQLVDGMYRDGFSDPLSGLLMGQT
                     AEKLARQYEITRQEQDEFALRSQQKAEATIASGRFNDEVMSLEISGKKGETTSFAKDE
                     HCRAGTTLADLAKLAPVFYKDGTVTAGNSSGITDGAAAVVVLSEEGIKRFGTTPQARI
                     VDYEITGVDPEIMGIGPVPAVRTLLKRQNLSLSDIDLIELNEAFAAQVIACDRELGFA
                     ADRLNVNGGAIALGHPIGCTGVRITTTLLYEMQKRAARFGLATLCVSGGMGLALLLER
                     V"
     gene            6483..7988
                     /locus_tag="VFH96_03370"
     CDS             6483..7988
                     /locus_tag="VFH96_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973035.1"
                     /translation="MLRRLTTTAQVESAYQEISALLREYAEWLFVCDGVSESLRREEI
                     EVLVERERLLVSCWTEKGTRLWRVVAWDWNGQLLELELSRRMRIQLIPRTAARVITAT
                     IRAARQMRCERLARLVAPVCFGSVVERMSLSRGPRPGQPGRYAQVLLRTRRERIAVVG
                     PVVSSAPAAVDAFLSSSLLWFRRTSVQQLWLVVSPELLKPLLYRIVLLRPGLKEIIRV
                     FTVDEDLTLLTEIAAPEKEDLWRRKVARFPPVAVATASELSLAIVAAAPEAIDIVHSR
                     HGETLRYFGLPFARVRSLLGHEKVWFGINRAHRCLLDESTANDWQNLLNDLRQHRSPS
                     ALDHRHAFYRAAPEAWLESLLRRDITKLDPGLIIAPLHAQFRTARGAKLGIRPIDLLA
                     LRQDGRLVVIELKVSEDREHVLQGADYWRRVEAHRRRGHIARAKLFGARTIRDEAPLV
                     YLVAPTLRVHPAFQTLAQCISSDIEIYRFDINEDWRTGVRVMRREKAQKNF"
     gene            complement(8068..9243)
                     /locus_tag="VFH96_03375"
     CDS             complement(8068..9243)
                     /locus_tag="VFH96_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET6973036.1"
                     /translation="MALKIDDLLRVACSHNASDLHLKVGAFPVMRIGGELHPVENAPR
                     LKPEDTLDMAFSMMSNRQKQRFKEVSEVDIGYGVSGLGRFRANIFQQRGTVSIVLRVI
                     PDHNKSVAQLGLPPVIEKIAEERRGLILVTGATGSGKSTTLASMIDCINSTRSGHIVT
                     IEDPIEFLHRDKQSFVTQREVDVDTRSFAEALRGALRQDPDVILVGEMRDHETIETAL
                     TAAETGHLVLSTLHTLDATETITRIVSSFPAHQQRSVRIQLAGILKAVISMRLVRAAK
                     GAGRVPAIEVLVSTAFIRDHIINEEKTYLIREAIAAGTSQYGMQTFDQSLFHLLHAGL
                     ITQEEALHNATNPDEFKMRVSGIYSKDDTLSHMTTPATAKVTPPPPSNEPDSIFVKH"
     gene            complement(9398..10447)
                     /locus_tag="VFH96_03380"
     CDS             complement(9398..10447)
                     /locus_tag="VFH96_03380"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET6973037.1"
                     /translation="MFAKDGKIKDVKTPRICDVVWHDGEFIKWNDARVHVMSHVLHYG
                     SSIFEGIRCYNTKQGPAIFRLREHIQRLLNSAKIYRMDHPWTLEQLCDASIELVKRSG
                     MDQCYIRPILFRSLDEEHPAFGVNPFPNPLACYIGAWDWGKYLGDEAIEQGVDVQVST
                     WNRLTANSMPAMAKSGANYMNSQLIKMEALLNGFAEGIALDDRGYVSEGSGENIFLVT
                     GDIVLTPPLSSSILPGITRDSVIQICEELGITVKERTIQRAALYVADELFFSGTAAEV
                     TPIRSVDRIPVGSGGRGPITAKIQKAFFEITKGERTAPGNWLTYVNSGKAAAADNNGF
                     STAALEADTAVITVK"
     gene            10769..11941
                     /locus_tag="VFH96_03385"
     CDS             10769..11941
                     /locus_tag="VFH96_03385"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET6973038.1"
                     /translation="MNHENQNTPVAETSATDPLSIHQKAIAIDMHVDTAQRLLDEHVD
                     IEQQLSDGHFDAVRAKAGGLDAQFFSIWVEPQLFGGGGAKCVKRADDQIAAVRALAEQ
                     HPETWVLATSAADIRRAASEGKIAALLGLEGGYAIDERLENVKRYYDLGVRYMSPAWS
                     VSTSWAGSSGDDVGATRGLNQFGKDVVREMNRLGMMIDVSHVSDPTFWDIVQTSTAPV
                     VATHSACRAIADVPRNLTDDMIRALAKTGGVVNVIFYPEHLEPGWSQKKKQVDAEIAS
                     AVQDASAAEPGDAVHKKLARDRVRQREYVRRLPPVTVARIVDHIDHIVKLVGVDHVGI
                     GSDFDGVQITTSDLATVADLPNLTKELLRRGYSASDVSKILGGNMLRVMEQLGGKR"
     gene            12043..>12887
                     /locus_tag="VFH96_03390"
     CDS             12043..>12887
                     /locus_tag="VFH96_03390"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET6973039.1"
                     /translation="MPRIGFHAPIKGGVHNALIVAKDTGCDTVQLFSRNPRGWMAKPL
                     TAEDVLLFKKTRKQTKISPVLIHCNYLVNLAAVDDVFLQKSRASFREEVERALLLGVD
                     YLVVHPGSSRGACEADGLGLCAASLQIACKGLRFGKLKILLENTAGQGDCIGHRFEHL
                     QELMRACPKLPLGVCLDTAHAFTAGYDIREEDGLQALIQRVQKTVKTKNVHAIHFNDS
                     RAAYNSRVDRHWHIGEGHIGADALRRVARHPKLKHAAFILETPYDDPRADLKNLEMLR
                     SFVEP"
BASE COUNT         2895 a         3437 c         3702 g         2853 t
ORIGIN      
        1 atttagcaca caggcgccgc agatggaaga agatttttaa acagatttgc acggatttta
       61 cgttatccgt taaaaatccg tgtaatccgt ggctatttat ccggaggcaa atcataatcg
      121 ggtggcaggt cgacagtgat cgtccgctcc ccttccgggg agacgaccag cgaagcgact
      181 accgacccaa tcagcacgag tgcgacgaaa acgagtgaaa tctttattgg aatgtggaat
      241 ccgatcgctc cgacgagcat tttcagcccg ataaaggtga gcacgatggc gaggccgaac
      301 cgcagatagc gaaacttctc caccacgccc gccagcagaa agtacatcga gcgcaggccc
      361 agaatcgcaa agacattcga cgtgtaaaca atgaatgtgt tggtcgtgac cgcaaaaatc
      421 gccggaatcg aatcaactgc gaacaccagg tcagtgactt cgacaaccat caggacgagc
      481 aaaagcagcg tgccggtccg ttttccgttt gctttggtga aaaatttctc ctcttcgtag
      541 tgccgggaga tcggaaggaa ccgcgtgact aaccggacga gggggttttt ctcaggttgg
      601 acatcgatct cgtcctgccg gaacatcttg attcccgtgt aaacgaggaa ggcgccgaaa
      661 atatagatga tccagtgaaa gcgattgatg agcgccgcgc cgacgaaaat catcgtcagg
      721 cgcataaaga gtgctcccat cacaccccag aagaggacgc ggtgttgaaa cttcgccggg
      781 actttgaagt aagaaaagat cagcaggaaa acgaaaaggt tatcgacaga gagcgataac
      841 tcgatgatgt agccggtgag aaactcgaac ccgggctgcg ttccgagata gtaaaaaact
      901 atcccagcga aaatcatcgc gagcgtcacc cacacgccgc tccagagcca cgcttctcga
      961 tatttaattg tgtgagcttt gcggttaaag agccccagat cgaggctcag catcgtgaaa
     1021 atgaatagac tgaatccgat ccacaaccac tttgagccgc caagtgcgtc catgaagtgg
     1081 attaaaatag catccggatc gtattccggc aaagcaagaa gtagccgcgg atgaacgcgg
     1141 atgaacgccg atcagacaag ccaattttga tccgcgttta tccgcgtcca tccgcggctc
     1201 taatttagtc cagtcccacc gctaccgcgc ttgggtcggt tgttgtacaa tccgcagttt
     1261 ctgaaaggta acaacaatgc tcaaacaact cgccaaatgg gaacgaacca gcaaatacat
     1321 cgtcataata tttgccgccc tgatggctat cagcctcgtg attttctatg cccccggacg
     1381 caactccggg aataacatcg atcccaccag aaacacggaa gtggtagcga aagtgggttc
     1441 cgccagtatc accgtcgctg acgtcgcgcg catcagggaa aactacgcgc agatgttcgg
     1501 caaccgcatc aacctggccc aactgggcgg taacaagcgc ttcctcgacg gcctgatcag
     1561 taagcaggtg atttctcagg aagccgcgcg cctcggtctc agtgccagcg atggcgagct
     1621 ccgcgagcgg atccgcaaac agttctccga tgcagctgga aactttgtcg gcttcgatag
     1681 gtacaaggag tccgtcacgg cgcgctacgg cgacatcgag aagtatgaga acgatattcg
     1741 cgacgagatc gctcaggaaa aactgcgcgc gttcgtgagt gcgagtgtca acgtttccga
     1801 tgctgaagtc gaagaagagt acaaacgtcg caacactacg ttcgacgtga actacgtggc
     1861 gctcacgagc gacaagctcg cgacgaagat tcagcccagc gatgacgagc tgcgggctta
     1921 ttacgacagt cacaaaaccg actatcgcta tctcgagcca cagcgaaaaa ttcgctacgt
     1981 ttttgttgac actgaaaagg tcggctcgaa gcttcagatt cccgatacgg acctgaaagc
     2041 ggagtacgac aagctcgacc cggagcacaa ggaagccggt atcgagatcc agcagattct
     2101 tttgaaagtc gcgcgcaagg atctggatgc gcaggttgaa cagaaagcga aagacctcat
     2161 caccaagttg cgtggcctga cgggccaggc gaccaaagaa caattcgccg aagtcgccaa
     2221 aggaaactca gaagatccgg cgaccgcgcg aaatggcggc atgctcgaga agcctttcaa
     2281 gaaaaacgaa aacaaagtta acggccttta cgatcgaact gttgacatga gaccgggaga
     2341 tgtcagcgac attccgattc gttatgccgg aaactggtac atcctccgcc gtggcgagag
     2401 tgttccgaag acttttgagc aggcgaagcc cgacctcctc gtctctttgc gcaaccgtaa
     2461 agcatatggc caggcgttcc aactggcgca gcaggcccag acgcgcttac aggaaaccaa
     2521 ggatccgcaa aaagtcgcgc aggagctggc tgctaaagcc aacatgactc cggcggagat
     2581 ggtgcgtgaa acgccgtttg tgaagcctgg cgacgacgtg ccccagatcg ggtccaatca
     2641 gcagtttgag caagccatcg aaacgttgaa caacccgaac gacgtcggta acgtgaccgg
     2701 tgtcaaaggc ggttttgcga ttccgatgct ggctgaaaag aaagatccgc gtattcctga
     2761 tttcgatgaa gtgaagacaa aggtcgccga agagatcaag aaacaacgtg cgaaagagca
     2821 gttggaacag aaagcaaaag acctcgtggc cagtctgact agtgccgacg gcatcaaggc
     2881 cgcgggcgag aaagaaggct atgaagccga cacggaagag tcgttcaaac tcggacaatc
     2941 gctcggcaaa gctggtacga gtgcagcgct tgatgatctg atctacagtt tgaaacccgg
     3001 cgagtttagc aagacgccga tcaaggttga cgataagtgg gtgatagtcg gcgtcacgaa
     3061 gcgaaacgat gctgacatga acgcccttgc cgcccagcgc gatacgttga agtcgtcgat
     3121 gatgagcgaa cgccaggaac aggtgtttga tgattacgtt gccggcgttg agcagcggat
     3181 gaagaaggac ggcaagatcg agatctaccc gaaggtgttg gcgcaactgg aagaggatga
     3241 gccggcggcc gagccgggtc tccccgccgg tttgaatttc ccaacgaaat aaatccgcgt
     3301 tttcaatccg cgtgaatccg cgttctagtt tccgagtagt ggcccaacct cagcctgggc
     3361 cacgctggtt ctgatttgca gctcgttcgc agccgaactg atcttcatat tcgacaaggc
     3421 gctcttcgcc agcacgcctt tcgctccgga taaacgtccc acgaacaacg cgccaaactg
     3481 cttcaaacct tcgagtgtgt ctccaaagtt cttcgcggcc tctgcattga ctgcgcgtgc
     3541 ggcaaggaac aactgcagat cggtttccgt ggtggcaacg gagccgtaaa cttgtcgcag
     3601 cgatttcaag tcagcaccaa tcggagcgtt gccgatatcg agattcgcca cgagctgttc
     3661 ggtgatattg ctgccaaagc cgatgactgc gttgggatcg cgattggcga gcgcgatcaa
     3721 ctctgcattc gctcgtttgc gcgaacggct cgcgtcgatc acactgcgca cgcccgtggg
     3781 atctccgagc gcgagggtgt ttgtatcgag cggcgtcgca gccagctcac cgatccggac
     3841 gtcgaataga ccaagtagtt ttacgtgctc atcaatcgag aaaatgtaga tggtcttgtc
     3901 ctgatatttt tcttcacggt actttccatt tgaagctact cgaccggcag cgaccattgc
     3961 cgcagcgctg aacgttccct cggcgagtgc gacagtttgc accttggtca cgccttcgga
     4021 gggatacgtg aaacgaatac cgagcgccac ctgctggaac atgcgcggat cgagaccggt
     4081 gcgatctttg aagcggtcga ggctcgcgtt gacttcgttg agcttcgcgg gattgccggc
     4141 gagtattcgt ggcatcgctt cgctgaggag ttgtttcact ttcacctgcg cgaccgcgtc
     4201 agattgcggc agtgaggaca tcagcgtcgg agctgcgggc gcgttgttgc ggctacgcgt
     4261 ttgcgccgca gcttcgagtt ggaaaagtgc gaccagaata atcgccaccg ttaaaagtcg
     4321 tgttttcact acaaacagcc tttctgcgaa gattttgaga gagttagtgg atgcctcgat
     4381 ggttcagtgg gttgttttca gcggaggcaa gcccctcttc ctgacttgtt ctttttcagt
     4441 tgttcattcc ttgagtcgcc gttggcaaga acaagtcagg aagaggggct tgcccccgct
     4501 gccctaatca ttcacggcca agcgtttccg aagctcagta aaagtgacct ttctataatc
     4561 atggggaatt gcaaatagac tcttatcgac ctcgagcttt atttccgaaa tctccatcgt
     4621 tacgtgtgtg ccgctggact tcgtctcgga tctgatgggc atctgcagtg cttcatcaat
     4681 ccacatcaag gtctcactct ggctgacgtt cgccggcgcc ggactgttaa caacgatgcg
     4741 atatttgttt gtattgcgac cagcaatcac ctcgttcccc agcttctgat aactcgtggc
     4801 gcctgcgtcc gtgtgcaaca gggcatcggg agaggatttc aattcctcat cgtcttgagc
     4861 atcggcaatc ttgctttcat ccgtgacatc cgcatacacc ttatcgtcgg gtagcaagaa
     4921 aaacctgcct tccggagtag cgagataaat ggtgttcttc agtgtgcctg ttgattcgta
     4981 acgactcgac tcgccatcac gcgcgatgaa ggcctttgta acgacggtcc ggccatcggc
     5041 actggcggta atggtgcgga cggcgctgta atgctcgggc tcctttgttt gaaatggtgg
     5101 agtcgacgag acggttgtgt cgggcgatgc ggttgcattc gtgcgggtat cggtttgcga
     5161 acgacagcca acgatgacga gcaggaccac acaaactacg acgaaccgca ggacgatcat
     5221 actcgctcca gcagcagtgc gagtcccatg ccgccgctga cgcacagcgt ggcgagccca
     5281 aacctggctg ctcgcttctg catctcgtaa agcagcgtcg tcgtgatgcg cacccccgtg
     5341 cagccgatgg gatggcccaa cgcgatcgcg ccgccattca cgttcaaacg atcggcagca
     5401 aagccgagct cgcgatcgca cgcgatcacc tgcgccgcga aagcttcatt caactcgatc
     5461 agatcgatgt cggaaagcga aagattttgt cgtttcagca gcgtacgcac ggcgggcact
     5521 ggaccaatgc ccatgatctc cggatcgacg ccggtgatct cgtaatcgac gatccgcgct
     5581 tgcggcgtgg ttccgaatcg tttgattcct tcctcgctca acacgactac agccgccgcc
     5641 ccgtctgtaa tacctgatga gttgccggct gtgacagtac cgtctttgta aaagacaggc
     5701 gccagctttg cgagatctgc gagtgtggtc ccggcgcggc agtgttcgtc tttcgcgaag
     5761 ctggtggtct cgcctttctt gccgctgatc tcgagcgaca tcacctcgtc gttgaatctt
     5821 cctgacgcga tagttgcttc tgctttctgt tgcgatcgca aagcaaactc gtcctgttcc
     5881 tggcgcgtga tttcatactg gcgcgcgagc ttctcagcag tttggcccat cagcagtcct
     5941 gaaagcggat cgctgaatcc gtcgcgatac atgccgtcga cgagttgcat gttgcccagg
     6001 cgcgcgcccc aacgagcgcc ctcggcgaag tatggcacgc gcgacatgct ttccgtcccg
     6061 ccggcgagca ccgcgtcggc gcgtccgagc attatctcct gcgccgcgag catgattgct
     6121 ttcagtcctg agccgcatgc ctggttgatg gtggcggcag gtgtcgtttg cgggacgccc
     6181 gcgcgaaaaa tgatctgccg cgccacgttt ggaccaccgc cggcctgacg cgcgcagccc
     6241 cagattgagt ctgtgatttg atcggctgcg acccccgcgc gacgcaggct ttcttttgct
     6301 actgcgacgc ctagatccgc ggctgtccat gatgcgagtg tgccgccaaa cttgccgatc
     6361 ggtgttctga ctgctccagc gagataaatg ttcttcataa ttcagttgac caaaaatgta
     6421 gcacaaggta gaagtaggcc acaaaaaggc acaaaaggca caaaaaaggc ggttgacctc
     6481 acatgcttcg acgcctcaca acgacggcac aagttgaatc tgcgtatcag gagatttccg
     6541 ctctcttgcg ggagtatgcg gaatggctgt ttgtgtgtga cggtgttagt gagtcgttgc
     6601 gcagggagga gattgaggtc ttagtcgagc gcgagcggtt gctggtttcg tgttggacgg
     6661 agaaggggac gcgattgtgg cgcgttgttg cgtgggattg gaatgggcag ttactggagt
     6721 tggaattgtc gcgccggatg cggattcagt tgatcccgcg gaccgccgcg agggtgataa
     6781 cggcgacgat tcgtgcagcg cgtcaaatgc gttgcgagcg gctggcccgg ctcgtggcgc
     6841 ccgtgtgttt cggaagtgtt gtcgaacgga tgtcgttgag tcgtggacca aggccgggac
     6901 agccgggacg ctatgcgcag gttcttttga gaacgagacg tgagcgaatc gctgttgttg
     6961 gtccagttgt gtcgagtgcg cctgcggcag ttgatgcgtt tctgtcgtcg tcgttgttgt
     7021 ggtttcgtcg cacgtctgtc cagcaactct ggttggtcgt atcgcccgag cttttgaagc
     7081 cgctgctata ccgcattgtg ctcttacgcc cggggttgaa ggagatcatc agggtgttca
     7141 cggtcgatga agatttaact ttattgacgg aaattgctgc tcctgaaaaa gaagatttgt
     7201 ggcgcaggaa ggtggcgcga tttccgcccg tagcagtcgc gactgcaagc gagcttagtc
     7261 tcgcaatcgt ggctgcagcg cccgaggcga tcgatatcgt gcattctcgt catggtgaga
     7321 cgttgcgata tttcggtctt ccattcgcgc gcgtgcgttc gctgctcgga cacgaaaagg
     7381 tctggttcgg aatcaaccgc gcacatcgtt gcttgcttga tgaatcaact gcgaatgact
     7441 ggcaaaacct gcttaacgac ttgcgtcagc atcggtcgcc gtcggcactc gatcatcgtc
     7501 atgcgtttta tcgtgctgcg ccggaagcgt ggctcgagtc gttgctgcgt cgagacataa
     7561 ccaaactcga tcccggtttg atcattgcgc cactgcatgc acagtttcgt actgcacgcg
     7621 gcgcgaaact cggcatcagg cccatcgatc tgctggcctt gcgacaggac ggccgactgg
     7681 ttgtgatcga attgaaagtc tcggaagacc gcgagcacgt gttgcagggg gccgattatt
     7741 ggcggcgcgt cgaagctcat cgccgccgtg gtcacatcgc gcgcgcaaaa ctcttcgggg
     7801 cacgaacgat cagagacgag gcgccgctcg tatatctcgt cgcgcccacc ttgcgcgtcc
     7861 atccggcatt tcaaacactg gcccagtgca tctcgtctga tatcgaaatc tatcgcttcg
     7921 acatcaacga agattggcgc acgggcgtcc gcgtcatgcg ccgcgaaaag gcacagaaga
     7981 atttttagcc acgaaaaggc acaaaagtca caaaagagaa atcctgaaga aaaccttttt
     8041 gtgacttttg tgccttttgt ggctaaatta atgcttgacg aagatgctgt cgggttcgtt
     8101 gctgggtggc ggtggggtga ctttcgctgt ggctggcgtg gtcatgtggc tgagggtgtc
     8161 gtccttcgag tagatacctg agacacgcat cttgaactca tccggattgg tcgcgttgtg
     8221 tagcgcctct tcctgcgtga tgagacctgc atgaagtaaa tgaaacaacg actgatcgaa
     8281 cgtctgcata ccgtactgcg aggtgccggc cgcaatcgcc tcgcgaatca aatacgtctt
     8341 ctcttcgttg atgatgtgat cgcgaatgaa cgcggtcgag acgagcactt cgattgccgg
     8401 cacccggccc gctcccttgg cggcacgcac gagtcgcatc gagatcacgg ccttcaaaat
     8461 tcccgcgagc tgaatacgca cagatctctg ctggtgtgca ggaaacgacg agacgatacg
     8521 cgtgatggtt tccgtggcgt cgagtgtgtg gagcgttgag agaacgaggt gtccggtttc
     8581 ggcagccgtc agcgcggtct cgatcgtctc gtgatcgcgc atttcaccga cgagtatcac
     8641 gtccggatcc tgtcgcaagg cgccgcgcag cgcctccgca aacgagcggg tgtctacgtc
     8701 aacttcacgc tgcgttacaa acgactgctt atcgcgatgc agaaactcga tcggatcctc
     8761 gatcgtgacg atgtgtccac tacgcgtcga gttgatgcaa tcgatcatcg atgcgagtgt
     8821 ggttgatttt cctgagccgg tggcgccggt gacgaggatc agtccccgtc gctcttcggc
     8881 gatcttttcg atcactggag gcagaccgag ttgcgcgacg gacttattgt gatcgggaat
     8941 gacgcgcagc acgatactga ccgtgccgcg ttgctgaaag atgtttgcgc ggaagcgtcc
     9001 gagaccggaa acgccgtaac cgatatcgac ttccgagact tccttgaatc gctgtttttg
     9061 ccggttcgac atcatcgaga aggccatgtc gagcgtgtct tccggtttca atcttggcgc
     9121 attttcgacg gggtggagct cgccgccgat acgcatcacg gggaaagccc ccaccttgag
     9181 gtggaggtcg gaggcgttat gactgcatgc aactcgaagt aaatcgtcga tttttagggc
     9241 catgatttta aggtggggtt agcgcgagat gtcgcgcttg ggctttttga atatagcttg
     9301 gcggtgtgag gtccgtcaat aaattctgat ccacagatta cgcagactac gcagattatt
     9361 ctatgaatta gtctgtgcaa tctgtggaat ctgtgaatta cttgaccgtg atgacggccg
     9421 tgtcggcttc aagcgccgcg gttgagaagc cgttgttatc ggctgcggcg gccttgccgg
     9481 agttcacata agtcagccag ttgccgggtg cggtgcgctc gcccttggtg atctcgaaaa
     9541 aggctttctg gatcttcgcc gtgatcggac cacgtccgcc gctgccgacc gggatgcgat
     9601 cgacggaacg aattggcgtg acttccgcag ccgtgcctga gaagaagagc tcgtcggcaa
     9661 cgtagagcgc cgcccgctgg atcgttcgct ctttaactgt gataccaagt tcttcgcaga
     9721 tctgaatcac gctgtcgcgg gtgatacccg gcagaatcga cgacgaaagt ggcggcgtca
     9781 aaacgatgtc gccggtgacg agaaagatat tctcgcccga gccttccgag acgtaaccgc
     9841 gatcgtcgag tgcgatacct tcggcgaatc cattcagcag cgcttccatc ttgatcagct
     9901 gcgagttcat gtagtttgcg ccgctcttgg ccatcgcggg cattgagttc gcggtcagtc
     9961 tgttccaggt ggaaacctga acgtccacac cctgctcgat cgcttcgtcg ccgagatact
    10021 tgccccagtc ccacgcgccg atgtaacaag cgagcgggtt cggaaacgga ttgacaccaa
    10081 acgccggatg ttcttcgtcg agactgcgga aaagtatggg ccggatgtag cactgatcca
    10141 tgccgctgcg cttgacgagc tcaatagacg cgtcgcacaa ctgctcgagc gtccaggggt
    10201 ggtccatgcg ataaattttt gccgagttaa gaagacgctg aatatgttcg cgcaggcgga
    10261 aaatggctgg tccctgtttg gtgttgtaac atctgatgcc ttcaaaaata ctggagccgt
    10321 agtgcaacac gtgcgacatc acgtgtaccc gcgcatcgtt ccatttaata aactcgccat
    10381 cgtgccaaac aacgtcgcaa atgcgtggtg ttttaacgtc cttaattttg ccgtcttttg
    10441 caaacatgtt gttactcctt caggaattac tgagatagag ggggaaggcc ctcatcttcg
    10501 ccgcgcagtt acacacttcg aacaaaaagg tacttctcga catgggctaa gagggttaat
    10561 caacaacgca caatacagta gtttcgagcc gcttggcaag aacgcttgcc tgctgtaaac
    10621 atgcttgcgt aacatgaaac ttgcgacacc ttaatttaga gattactaat tcgtgggctt
    10681 acccaaaaac cgttcgaaaa ctctcacaat tgctgtcgta tttatcattc tgccctcgat
    10741 acttcttccc ggctgtacgc ttaaaaacat gaatcacgaa aaccagaata cgccagtggc
    10801 ggaaacgtct gccacggacc cgctttcaat ccaccaaaag gccatcgcca tcgacatgca
    10861 cgtcgatacg gcgcagcgct tactcgacga gcacgttgat atcgaacagc agctcagcga
    10921 cggccacttc gacgcggtac gcgcgaaagc cggcgggctt gatgctcagt ttttctcgat
    10981 ctgggtcgag ccgcaattgt ttggcggcgg tggcgcgaag tgtgtgaaga gagccgacga
    11041 ccagatcgct gccgtgcgcg ccctcgcgga gcagcatccc gagacgtggg tgctcgcgac
    11101 ttccgctgcc gacatccggc gcgcggcgag tgagggaaaa attgcggcct tgctggggct
    11161 tgaaggtggt tacgcgatcg acgagcggct cgaaaatgtg aagcgctatt acgatctcgg
    11221 ggtgcgctac atgtcaccgg cctggagcgt gagcacgagt tgggcgggct cgtccggcga
    11281 cgatgtgggc gcgacgcgcg ggctgaatca gtttggcaag gatgtcgtgc gcgagatgaa
    11341 tcgtctgggg atgatgatcg acgtttcgca cgtttccgat ccgactttct gggacatcgt
    11401 gcagacgtct acggcgcccg ttgtcgcgac gcattcggcg tgcagggcca ttgcggacgt
    11461 tccgcgaaat ttaaccgacg atatgattcg cgcgctggcg aagaccggcg gcgtggttaa
    11521 cgtgatcttt tatcccgagc atctcgagcc tggttggagt cagaagaaga agcaggtcga
    11581 tgcggagatc gcgagcgcgg tacaggatgc gtcggctgct gagccgggtg atgcggtgca
    11641 taagaagctc gcgcgcgatc gagtgcggca acgggagtat gtgcgacgac taccgccggt
    11701 gacggtcgca cgcatcgtgg accacatcga tcacatcgtg aaactcgtcg gtgtggatca
    11761 tgtcggcatc ggatctgatt ttgatggcgt acagatcacg acctcggatc tcgcgacagt
    11821 agctgacctg ccgaacctga caaaggagct cttgcgccgc gggtatagcg cgagtgatgt
    11881 gagtaagata cttggtggga atatgttgag agtgatggaa cagcttggag gtaagaggta
    11941 gtttttttag acaggattta caagattaca gaattaacaa gaaagaatct tgcttatcct
    12001 gaaatcttgt aaatcctgtc taaaactatc gtcgtcgtaa tcatgcccag gattggtttt
    12061 catgctccta tcaaaggagg cgtgcacaac gcgctgatcg tggcgaagga cacgggttgc
    12121 gatacggtgc agcttttctc gcggaatccg cgtggctgga tggcgaagcc tcttaccgcc
    12181 gaagacgttc tcctatttaa aaagacacgc aagcaaacaa agatctcacc ggtgttaatc
    12241 cattgcaatt acctcgttaa cctcgccgcc gttgacgatg tgtttttgca gaagtcgcgc
    12301 gcatcgtttc gcgaggaagt cgagcgggcg ttgttgttgg gtgttgatta tctcgtcgtg
    12361 catccgggaa gttcgcgtgg tgcgtgtgaa gcggacggcc tcggtttgtg cgcggcgagt
    12421 ttgcagatcg cgtgcaaggg cctgaggttt ggcaaactca aaatccttct cgaaaacact
    12481 gccggacaag gcgactgcat cggccaccgt ttcgagcatc ttcaggagct catgcgcgct
    12541 tgtcccaagc tcccgctcgg tgtttgtctc gataccgcgc acgccttcac cgcgggctac
    12601 gacattcgtg aagaagacgg ccttcaagct ttgatacaac gagtgcaaaa gacagtgaag
    12661 accaagaacg tccacgcgat tcacttcaac gactcgcgcg cggcgtacaa ctcacgcgtc
    12721 gatcgccact ggcacatcgg cgagggccac atcggcgccg acgccctgcg ccgcgtcgcc
    12781 cgccacccaa aactaaagca cgcggccttc atcctcgaaa ctccctacga cgacccgcgc
    12841 gccgatctca agaatctcga gatgctacgt tcctttgttg agccaca
//
LOCUS       DASSFQ010000104         5072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1682351, whole genome shotgun sequence.
ACCESSION   DASSFQ010000104 DASSFQ010000000
VERSION     DASSFQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682351"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFH96_03395"
     CDS             complement(<1..428)
                     /locus_tag="VFH96_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6973040.1"
                     /translation="MVAELERDVEARSAVFKKELGLFDLVLAQVVFVVGTIWVGWAAK
                     LGYDQNVFWLLAIATYYLPLAAAVIFLNRLAPLEGGLYQWAKLAFNDFVAFMVAWNLW
                     VFAISILAGIGLVVTTNFSYAIGPAGVWMREHRILITVIS"
     gene            complement(413..1087)
                     /locus_tag="VFH96_03400"
     CDS             complement(413..1087)
                     /locus_tag="VFH96_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6973041.1"
                     /translation="MADPNIDRTLTARINLYYDLHRPSAAPAPLLIALHGYGANKRQM
                     MREAQQMAPDGFAIASLQGFHQHFREPREPGGPARFGFGWLTSYRSDESVAIHHQALL
                     DLISQLVEEGVADPTRIFLLGFSQSCALNYRFAFTHPERLRGVIGICGGIPSDWDENP
                     SYKVSQLDVFHLAGSRDNFYPPVRVRDYERQLATRARSVKFKTYDATHDFVPPMREDV
                     KQWLQN"
     gene            1230..1547
                     /locus_tag="VFH96_03405"
     CDS             1230..1547
                     /locus_tag="VFH96_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET6973042.1"
                     /translation="MKFPGGFNMQAMMKQAQQMQEKMQKEMEELRVDASAGGGVVTVQ
                     MKGNHEIISIKIDPEAVKDGDVEMLQDMIVAAANEANRKVDEAIKGKIGGMLPPGLGG
                     LFG"
     gene            1557..2171
                     /gene="recR"
                     /locus_tag="VFH96_03410"
     CDS             1557..2171
                     /gene="recR"
                     /locus_tag="VFH96_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET6973043.1"
                     /translation="MLEYAEPVTKLIDELKRLPSIGHKSAQRLAFHIMRIQDSDLERL
                     IAAMRDVKLRIVLCSVCNNLTEVDPCRFCASPSRDHSVICVVEEPHSLVAVEKTRSFK
                     GVYHVLHGSLSPIRHIGPSELHLANLLPRLKPENNDGVKVTEVILATNPTTEGEATAN
                     YISRLLKPLGLRVTRIAMGMPVGSDLEYVDEVTMDKALANRHEI"
     gene            2207..3034
                     /locus_tag="VFH96_03415"
     CDS             2207..3034
                     /locus_tag="VFH96_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973044.1"
                     /translation="MKPTILLLLACVLAVSISAQRVDSLDRTAESSIKVGRDKDGNRV
                     LTTINRRFTLTESVRPLLLLEEFRHDLEAGHEQAKGVVKVDAWRQPDLNKKAWSIEQS
                     GDEGRVADEFYVVTKYGCCMSPTTSVYFNIDNGERIVSATDKLSSVIIPNTDQYRYVA
                     WHSAYASIPPTETHETDLQGVLQYVSYKAPLWKLAIYSKRDVNVRIKLRYEGKLVESE
                     SLMLWGVDGKRDKSSLSNFEIVLSMDSAGEIVLPITNDEVDLTRATIPPLFRVEVVK"
     gene            complement(3037..3729)
                     /locus_tag="VFH96_03420"
     CDS             complement(3037..3729)
                     /locus_tag="VFH96_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET6973045.1"
                     /translation="MRPRMEQMGVIDVCSRRATTKHKLENDMVELIPSQEAVMQILKR
                     TGAFREGHFIYPNGKHSPHYFQMPLAFRYYDTARVLAVALSRKFRLEKDISSQLPKVS
                     IISPSSGGIPVAFGVRDALNAEQIYWAEQERTGERMFRQYVNQGEVNPCIIVDDIIRS
                     GNAIEETVDLVKSLGARIIGCGAIVRFDSAPSEVEGVPIKSLVEFDVRMYDTGAECVE
                     CKDGASPETVRY"
     gene            complement(3695..4246)
                     /locus_tag="VFH96_03425"
     CDS             complement(3695..4246)
                     /locus_tag="VFH96_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6973046.1"
                     /translation="MYTKSAADLHGQTQTGKPVYARCVLQKFIARVWRALPPRVRRWS
                     MRATQTRFTVTAGALIFNDAGQILLLKHRFRAGSGWGIPGGFLEYSEQPEQALRRELR
                     EEIGLEVSQVEIFTARSFEKPRQVEILFRCHANASAAQVKPHTMEVEHAEWFSPQKLP
                     AGLPTDQRLLIERATAYGANGRD"
     gene            4261..>5072
                     /locus_tag="VFH96_03430"
     CDS             4261..>5072
                     /locus_tag="VFH96_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973047.1"
                     /translation="MAATETIAVPKRITNAQTVCNEKNEKGKLCNGHLKQLRTAGEPA
                     QVHLRGDDVLFKCQTCGTLYMGPPLGHVRDPNKQVRFVEAELTALLQAAGGTLPVIKK
                     DEQRGVFVMVETVAGDHGAHAAKPAAAKPAAPAAPKAAAAAPAAQKPAAPAAPKAGEH
                     KTLGQNPNRQLFRRSTYVGVKDTGPVAGETFEQKVARLKAVSVAAKQRADEGGGESAS
                     LAHDASSEAGLASPTSAAPSSPDTNAADAVVQSAEITAPPPAPASSPAHKPTG"
BASE COUNT         1156 a         1423 c         1450 g         1043 t
ORIGIN      
        1 aaactgatca cggttatcag aatgcggtgc tcgcgcatcc agactcccgc tggtccaatc
       61 gcgtacgaaa agtttgtcgt gactacgaga ccgataccgg cgaggatcga gatcgcaaac
      121 acccaaagat tccacgcgac catgaacgcg acgaaatcgt tgaacgccag cttggcccac
      181 tgatacagac cgccctcgag cggcgcgaga cgattgagaa agatgactgc cgcggcgagc
      241 gggagatagt aagtggcaat ggcgagcagc cagaaaacgt tctggtcgta gccgagcttc
      301 gcggcccagc cgacccagat cgtgcccacc acgaacacga cctgcgccaa aacgagatca
      361 aacagtccga gctctttctt gaagactgcg ctacgagcct caacatcgcg ctctagttct
      421 gcaaccattg cttcacgtcc tcgcgcatcg gtgggacaaa gtcgtgcgtc gcgtcatagg
      481 tcttgaattt aacgctgcgc gctcgcgtgg cgagttgtcg ttcgtaatcg cgcacacgca
      541 caggtggata gaagttgtct ctgctgcccg ctaaatgaaa gacgtccagt tgtgaaacct
      601 tgtagctggg attctcgtcc cagtcgctcg gtatcccgcc gcaaatgccg atcacgccgc
      661 gcaagcgttc cgggtgagtg aacgcaaatc gatagttgag cgcgcacgac tgcgagaaac
      721 cgaggagaaa gattcgagtc gggtcggcaa cgccctcttc gaccaactgt gagatgagat
      781 ccaatagagc ctggtgatgt atggcaacgg attcgtcgct gcggtagctc gtcaaccagc
      841 caaaaccaaa ccgcgctgga ccacccggtt ctcttggttc tcggaagtgc tgatgaaagc
      901 cctgcaacga cgcgatcgcg aaaccgtcgg gcgccatctg ttgcgcctcg cgcatcatct
      961 gtcgcttgtt tgcgccgtag ccgtgcaacg cgatcaatag cggcgcgggc gcagcagagg
     1021 gacggtgcag gtcgtaataa agattgatcc gagccgtcag ggtacggtca atatttggat
     1081 cagccatagt tctgggctct cattctgtgg attctggcca aggtgtcaat agctgacgct
     1141 tgaattgaaa actgttggcg gctaggatgg cttcaatttc ggtcttcaat cagagcgagt
     1201 ttggttaatt ctttcgcaga ggtgtcgcaa tgaaatttcc gggcggattt aacatgcagg
     1261 cgatgatgaa gcaggcgcag cagatgcagg agaaaatgca gaaagagatg gaagagctgc
     1321 gtgtcgatgc ttcagcggga ggcggcgtcg tcaccgtaca gatgaaaggc aatcatgaga
     1381 tcatttcgat caagatcgat cccgaagcgg tgaaagacgg cgacgtcgag atgcttcagg
     1441 acatgatcgt ggcggcggca aatgaagcga accgcaaagt ggacgaagcg atcaaaggga
     1501 agatcggcgg gatgttgccg cccggactcg gaggcctctt cggttaatag ccagcgatgt
     1561 tggagtacgc agaaccagta accaagctca tcgacgaact gaaacgcctg ccgagtatcg
     1621 gacataagtc cgcgcagcgt ctggcgtttc acatcatgcg cattcaggat tctgatttgg
     1681 aacgtttaat cgccgcgatg cgcgacgtaa aactgcggat cgttctttgt tccgtctgca
     1741 acaacctcac ggaagtcgat ccgtgccggt tttgtgcgtc accgagccgc gatcactcgg
     1801 tgatctgtgt ggtcgaagag ccgcacagcc tggtggccgt ggaaaagact cgcagcttca
     1861 aaggcgtcta tcacgtgctg cacggcagtc tctcgccgat tcgccacatc gggcccagtg
     1921 agctgcatct cgcgaatttg ttgccaagat tgaaaccgga gaacaacgac ggcgtgaaag
     1981 tgacggaagt aattctcgct acgaacccaa ccaccgaagg cgaggccacg gcgaactaca
     2041 ttagtcggtt gctgaagcct ttgggattgc gcgtgacgcg aattgcgatg ggaatgccgg
     2101 tcggcagcga cctcgaatac gtggatgaag tcacgatgga taaagcgctc gccaaccggc
     2161 acgagatctg aagcaactct actaacttca tgaggggtaa actgccatga agccaactat
     2221 tcttctcctg cttgcttgtg tcctcgctgt ttctatttcc gcccaacgtg tggattcact
     2281 cgatcgcact gccgagtcgt ccatcaaggt tggccgcgac aaagacggca atcgcgtgct
     2341 cacgacgatt aaccggcggt tcactctcac cgaatccgtg cgaccgctgc ttttgctcga
     2401 agaattccgc catgaccttg aagccgggca cgagcaggca aaaggtgtcg tgaaggttga
     2461 tgcctggcgt caaccagacc tgaataagaa agcatggtcc atcgaacaga gcggcgatga
     2521 gggtcgcgtg gctgatgaat tctacgtcgt taccaagtat gggtgttgta tgtcacccac
     2581 aaccagcgtc tacttcaaca tcgacaacgg tgagcgaata gtctcggcga ctgacaaact
     2641 gtcgagtgtg attattccta ataccgatca ataccgatac gtcgcctggc attccgccta
     2701 cgcatcaatc ccgccgacgg aaactcacga aactgatctg caaggggtgc ttcagtatgt
     2761 ctcatacaaa gcgccgcttt ggaagctcgc gatctattcc aaacgagacg tcaacgtacg
     2821 tatcaagctt cggtacgaag gcaagttggt ggagtctgag tcgctgatgt tgtggggtgt
     2881 ggacggcaag agagacaaat cgtccctctc caattttgag attgtgctgt cgatggattc
     2941 cgccggcgag atcgtgctcc cgatcaccaa cgatgaggtc gatctaacca gggccacgat
     3001 tccgccgttg tttcgcgtcg aggtcgttaa gtgatcttaa taacgaaccg tctcgggtga
     3061 cgcgccgtct ttacattcga cacattcggc gcccgtgtcg tacatccgca cgtcaaactc
     3121 caccagcgac ttgatcggca cgccttcgac ttcactcggc gccgaatcaa aacgaacgat
     3181 cgcgccgcaa ccgatgatgc gcgcgccgag cgacttcacc aagtcaaccg tctcttcgat
     3241 cgcgttgccg ctgcgaataa tatcgtcgac gatgatgcac ggattcactt cgccctgatt
     3301 gacgtactgg cggaacatcc gctcgccggt gcgttcctgt tccgcccaat aaatctgctc
     3361 cgcgttgagc gcatcacgca caccaaacgc gaccggaatt cctcccgagc tcggactgat
     3421 aattgagact tttggcaact gactggagat gtctttttca agcctgaact tgcggctcag
     3481 cgccactgcc agcacgcgcg ctgtgtcgta gtagcgaaaa gcgagcggca tttggaagta
     3541 gtgtggcgag tgtttgccgt tgggataaat gaagtgacct tcgcgaaacg cgccggtgcg
     3601 tttcagaatc tgcattacag cttcttggga aggaatcagc tcgaccatgt cgttctccag
     3661 tttgtgtttg gtagttgcgc gccgagagca tacatcaatc acgcccattt gctccatacg
     3721 cggtcgcacg ttcgatgagc aggcgttgat cggtcggcaa accggcgggg agtttttgtg
     3781 gtgaaaacca ctcggcgtgt tccacttcca tcgtgtgcgg cttcacttgt gcggctgatg
     3841 cattggcgtg acagcgaaaa agaatctcga cctgacgcgg cttctcaaac gagcgcgcag
     3901 tgaagatctc cacctggcta acttccagac cgatctcttc gcggagctca cgccggagtg
     3961 cctgttccgg ctgttcgctg tattcgagaa agccgccggg aatgccccaa ccgctcccgg
     4021 cgcgaaagcg atgtttgagc agtaatatct ggccggcgtc gttgaagatc agcgcgccgg
     4081 cggtgacggt gaaacgcgtt tgcgtggcgc gcatgctcca gcggcgcacg cgcggcggca
     4141 gtgcgcgcca gaccctggcg atgaactttt gcaacacgca ccgagcatac actggttttc
     4201 cagtctgtgt ttgaccgtgc aaatctgcgg ctgacttcgt gtacaatgcg attcaccacc
     4261 atggcagcca ccgaaaccat cgcggttccg aaacgaatca ccaacgcgca gaccgtctgc
     4321 aacgagaaaa acgagaaggg caaactctgc aacggtcacc tgaaacagtt acgcacggca
     4381 ggtgagccgg cgcaggttca cttgcgcggc gacgacgtgc tcttcaagtg tcaaacgtgc
     4441 gggacgcttt acatgggtcc gccgttgggc cacgtgcgcg atccaaacaa gcaggttcgt
     4501 ttcgttgaag cggagttgac tgcgttgttg caggctgcag gtggaacgct gccggtgatt
     4561 aagaaagacg agcagcgcgg tgtgtttgtg atggtcgaga cggtcgccgg cgatcacggc
     4621 gctcatgctg cgaaacccgc ggctgcgaaa ccggccgcgc ctgcagcgcc taaagcggca
     4681 gccgccgcac ccgctgcgca gaaaccggct gcgcctgctg cgccgaaagc gggcgaacac
     4741 aagactctcg ggcagaatcc gaaccgccaa ctcttccgtc gctcaactta tgttggcgtg
     4801 aaagacaccg gccccgtcgc tggtgagact ttcgaacaaa aagtcgcgcg cttaaaagca
     4861 gtctcagtcg ccgctaaaca acgcgctgac gaaggtggtg gtgaatcggc gtccttagcg
     4921 cacgacgctt catctgaagc gggattggcg tccccaactt cagccgcgcc ctcctcgccg
     4981 gacacgaacg ctgcagacgc ggtcgttcaa tccgctgaaa ttaccgcacc tccgccagcg
     5041 ccggcttcat ccccggcaca caagccgacc gg
//
LOCUS       DASSFQ010000105         6001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_783655, whole genome shotgun sequence.
ACCESSION   DASSFQ010000105 DASSFQ010000000
VERSION     DASSFQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783655"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1461
                     /locus_tag="VFH96_03435"
     CDS             238..1461
                     /locus_tag="VFH96_03435"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HET6973048.1"
                     /translation="MPDLHELHRHARAIFHHALTSVDPRPITKDAITNLNISTPIYSI
                     AIGKAATSMALGLDDAISDRLSAGVIVSTSLHQSTRWQSFIGGHPLPNESSLAAARAA
                     FALLDRANNEQVTIIFLISGGGSAMIEWPISDDISLDDLRSANQNLVLCGASISEINS
                     VRRAFSAVKGGGLARRASHTRMFTLIVSDTNPGDETSVASGPTLNPPAPKAIEVVEHY
                     HLETVLPESIMKAVRAQHSPEPITGSSQVLLDNQTALDAAAQKARELGYECATLDHIR
                     EQPIEKGCELLLEHTRDTCTISGGEFSCPVHGDGRGGRNLETTLRCAININTRSHLVV
                     LSAGTDGIDGNSPAAGAIADETTLQRARNLNLDAKQYLARSDSYTFFEQLNDLIVTGP
                     TGTNVRDLRIVLRSV"
     gene            1506..2036
                     /locus_tag="VFH96_03440"
     CDS             1506..2036
                     /locus_tag="VFH96_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HET6973049.1"
                     /translation="MPAVSTLTFTDLIERLNSQASPPTLEQLNDWVSSVEITDLDLEP
                     YCGFKEGNYWRHRVCRNAAVEMLVICWRPGQKTPIHDHNGSHGVVKVQKGLMWETIFT
                     YQEDRGLCYDTGRECPTGTVTGADLPDIHRLGNPDDSGQDLITVHIYAPPLGVLKTYK
                     VGSSQIDLYTPNDFPT"
     gene            2020..2805
                     /gene="modA"
                     /locus_tag="VFH96_03445"
     CDS             2020..2805
                     /gene="modA"
                     /locus_tag="VFH96_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6973050.1"
                     /translation="MISRLETRLAPLAAFVLALACVTACKDKTPATPIASSGGELTVA
                     AASDLTPVFEELGREFESATKTKVVFSFGSTGTLTRQIENGAPFDLFAAANVSYIDEL
                     DQKGLIIPDSRAIYARGRITLWTANESPVRFQGIADLAQPEIQRIAIANPDHAPYGLA
                     AKQALQSAGIWDRVQPKLVYADNIRQTMQYAQTGNVEVAIVSLTLSINSNGRWTLIPE
                     ELHQPINQGLAIMKSTKNEQAARAFITFLSSPQGKAIMKKYGF"
     gene            complement(2905..5508)
                     /locus_tag="VFH96_03450"
     CDS             complement(2905..5508)
                     /locus_tag="VFH96_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET6973051.1"
                     /translation="MRPHSNLFTQHPLVHLAVAFSAGICTASYLSLRFSLAAGIASTT
                     VMLILFVRQRLRLAVLSLLSALFFTGVTLAELERRVDQARALKNLVEQSEGEPFTLTG
                     WLDGPPEFARDRVYLSLRVENVAGRVSLLVTLRDINEFRALQLRYGTRIQVITTLDRD
                     GNYRNPGVSSLAEFLDRNGYDASGLIKSPTAITRLGDTRVFPPLAWLYDWRSRLQQQI
                     DTSFQPETAGVLDATLLGNRYNLSTTASERFREGGTFHVLVISGLHISFIGAVVFLIV
                     KRLTRRRLLQFVLPALIVWAYSFAVGADASVVRAALMFTFAGVATILFRQSSSLNALG
                     GAALVLLVHRPKELFDPSFQLTFLSVLAILVIAWPLLSNMSAIGAWYPTRYSPYPPMC
                     ARGFKYICEVLFWREKRWAQEIARSTHDYHLFKAPVALRLERYYVQPVLRYTFGAVVV
                     SASVQLMLLPLMIVYFHRLSLASLVLNIVVSILLAVLVVVALLALMIGQVPLFKLADA
                     INWLMVHSVDPFSDFGWAGFRLPEYSGPAALIYAIYYLPLLILIIALLHWRPMALQAE
                     RRCKLHAYVLPLTCAQLLLLTVLILHPFSSRSDARLRIDFLDVGQGDSALVTMPDGTT
                     LLVDAGGNTMDSTRRIGETVVSEYLWRRGLSEIDYVLATHADADHIDGLNDVLKNFTV
                     RSALIARSPADDPEFAKFAQTLARTNTHSETIEAGDVIYFGDVIVSVLWPSAGGEKST
                     NNDSIVLRIQYGERSILLTGDIEQAAERALIASPQQLHADVIKVPHHGSKTSSTDAFV
                     SATNPQFAIISVGRTSRFGHPHKEVVERWQSSGATVLTTGHSGTITITTDGHDLSLKT
                     LLQPQKGTKNF"
     gene            complement(5596..>6001)
                     /locus_tag="VFH96_03455"
     CDS             complement(5596..>6001)
                     /locus_tag="VFH96_03455"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HET6973052.1"
                     /translation="VGLFTLPLSRQFQRVTAVEANPVAVRFARRNLQDARLANARVIA
                     AGVADWFRSAPVREVKFVLLDPPRAGAESAVIKGILDLHPPQISYVSCDPATLARDLR
                     KLVAGGYTISSIRGFDLFPQTHHVETVVRLTV"
BASE COUNT         1482 a         1748 c         1563 g         1208 t
ORIGIN      
        1 ttgcagcgtc gtctgcgcat cggctacggc cgcgcggcag caatcctgga catgatggaa
       61 cgcgaaggct tgatcgggca ggcagacggc tcgcgtcctc gtcctgtgct tggccgcgcc
      121 tacgagatgg tcgccgactg ggacgagatg aacaccgctg acgtgtgagg ttctttatcc
      181 acagattaca cagattcaat taaacaatct gtgtaatctg tggatgggtt tctgactgtg
      241 cccgatctac acgaactgca ccggcacgct cgcgcgatct ttcatcacgc cctcacctca
      301 gttgaccctc gcccgataac aaaagacgct atcaccaatc tcaacatctc cactcccatc
      361 tactcaatcg ccatcggtaa agccgccacc tcgatggccc tcggcctcga cgacgctatc
      421 agcgacaggc tatctgccgg ggttatcgtc tcaacctcgc ttcaccaatc aacacgctgg
      481 caaagcttca tcggcggcca tccacttcca aacgaatcaa gcttagctgc cgcacgcgcc
      541 gcttttgcgt tactcgaccg cgccaacaac gaacaagtaa caattatctt cctgatctcc
      601 ggcggcggct cggcaatgat cgagtggccc atcagcgacg acatctcact cgacgatctt
      661 cgttcagcaa accaaaatct cgtcttgtgc ggcgcaagca tctcggaaat caactcagtg
      721 cgtcgcgcct tctcagcagt aaagggcggc ggactcgccc gccgcgcctc acacacacga
      781 atgttcacgt tgatcgtctc cgatacgaat cccggcgatg aaacgagcgt cgcctccggc
      841 ccaacactca acccaccggc accgaaagcg atcgaagtcg tcgaacacta tcatctcgag
      901 acagtactgc cggaatcgat catgaaagcc gtacgtgcac agcactcacc ggaacctatc
      961 accggctcat cccaggttct tctcgacaac caaacagccc tcgacgccgc cgcacaaaaa
     1021 gcacgcgagc tcggctacga atgcgcaacg ctggaccaca tccgcgaaca accaatcgaa
     1081 aaaggctgcg aattgcttct cgaacacacg cgagacacct gcacgatctc aggcggcgaa
     1141 ttctcctgtc ccgtccatgg cgacgggcgc ggcggccgaa accttgagac tacgctgcgc
     1201 tgcgccatca atatcaacac acgctcacat ctcgtcgtcc tcagcgccgg cactgacggt
     1261 atcgacggta acagccccgc cgccggcgcc atcgccgacg aaacaactct ccaacgcgct
     1321 cgaaacctga atctcgacgc caaacaatat ctcgcacgca gcgactccta caccttcttc
     1381 gaacaactca acgacctgat cgtcaccggc cccaccggca caaacgttcg cgatctcagg
     1441 atcgtactca gatccgtgta aatctgcttt ttaaaatccg tccaatccgt gttagattcc
     1501 aaacgatgcc agccgtatcg acactgacat ttaccgacct aatcgaacgc ctgaacagcc
     1561 aagccagtcc gccaacgctc gaacagttga acgactgggt ttccagcgtc gagattaccg
     1621 atctcgacct cgagccttac tgcggtttca aggaaggcaa ttactggcgc catcgagttt
     1681 gccggaatgc agccgtcgag atgctggtga tctgctggcg cccaggccag aagacgccga
     1741 ttcacgatca caacggctca cacggtgtcg tgaaagttca aaaaggcttg atgtgggaaa
     1801 cgatttttac gtaccaggaa gacaggggtt tgtgttacga cacgggtcgc gaatgtccga
     1861 ccggcaccgt cacgggcgcc gatttaccgg acattcatcg actgggaaat cccgacgata
     1921 gtggtcagga cctcatcact gttcatattt atgcgccgcc gctcggtgtg ttgaaaactt
     1981 acaaggtggg tagttcgcag atagaccttt acacgcccaa tgatttcccg acttgagacg
     2041 cggctggcgc cgctcgccgc gtttgtacta gcgctggcgt gcgtcaccgc gtgcaaggat
     2101 aaaactcccg cgactccaat tgcttcttcc ggtggcgagt tgactgtcgc cgcagcttca
     2161 gatctcacac ccgtattcga agagctcgga cgcgagttcg aatcagctac caaaacaaag
     2221 gtcgtgtttt cgtttggctc gactggaacg ctcacgcgcc agatcgaaaa tggtgcgccg
     2281 tttgatctct tcgcggcggc caatgtgagc tatatcgatg agttggacca gaagggactg
     2341 atcattccgg actccagggc gatctacgcg cgcgggcgca tcactctgtg gacggcgaat
     2401 gagagtcctg tacggttcca gggtatcgct gatctcgcgc aacctgagat acagcgcatc
     2461 gcgatcgcga atccggatca cgcgccgtac ggacttgccg cgaaacaggc actgcaaagc
     2521 gcgggcatct gggatcgtgt gcagccgaag cttgtttatg ccgacaacat tcgccagacg
     2581 atgcagtatg cgcagacggg aaacgtcgaa gtcgcgatcg tttcgttgac gctgagcata
     2641 aacagcaacg gccgctggac gctgatcccg gaagagttgc accagcccat caaccagggc
     2701 ctcgcgatca tgaagagcac taagaacgag caagccgcac gcgcctttat caccttcctc
     2761 agctcgccac aaggcaaagc catcatgaag aagtacgggt tttagccaca aaaaggcaca
     2821 aaaagcacaa aataagaaag cagcaagagg ttcacgcgac tccctctcag aaaacctttt
     2881 tgtgcttttt gtgccttttc gtggctaaaa attctttgtg cctttttgtg gctgaagaag
     2941 ggttttgagg ctgaggtcgt ggccgtcggt ggtgatagtg atcgtaccgc tatgaccagt
     3001 cgtcaaaact gtggcgccgc tggattgcca gcgctcgaca acttctttat gtggatgtcc
     3061 gaagcgcgag gtgcgtccga ctgagatgat tgcgaattgt gggttagtcg cactcacgaa
     3121 agcgtctgtt gaagaggtct tgctaccgtg atgtgggacc ttgatcacat cagcgtgcag
     3181 ttgttgtggc gaagcgatta gtgctcgctc ggcagcttgt tcgatatcgc ccgtgagtaa
     3241 tatcgagcgc tcgccatact gaattcgaag cacgatcgaa tcgttgttcg tcgacttctc
     3301 accaccagca gacggccaca gcacactaac tatgacgtct ccgaagtaaa ttacatcacc
     3361 ggcttcaatc gtttccgaat gtgtgttcgt gcgcgcgagt gtttgcgcga acttcgcgaa
     3421 ctcgggatcg tcagcagggc ttcgcgcgat caacgcagat ctgacagtga agtttttgag
     3481 aacatcattc aggccgtcga tgtgatcggc atcagcgtga gtcgcgagga cgtaatctat
     3541 ctcgctcaag cctcgccgcc agagatattc cgacacgaca gtctcaccaa tcctgcgtgt
     3601 tgaatccatc gtgtttccac cagcgtcgac cagcagcgtg gttccatcag gcatcgtcac
     3661 gagcgctgaa tctccctggc ccacgtcgag gaagtcaatc cgcaaacgag catcagatcg
     3721 gctgctgaat ggatgcagga tgagaacagt aagcaggagc agttgcgcac aagtgagtgg
     3781 gagaacgtac gcgtgcagtt tgcaacgacg ttcagcttgc agggccatcg ggcgccagtg
     3841 caataacgcg atgatcaaaa tgagtaatgg taagtaataa atagcgtaga tcagtgcggc
     3901 gggacctgaa tactccggca gcctgaatcc cgcccaaccg aaatccgaaa acggatccac
     3961 actatgcacc atcagccagt tgatcgcatc tgcaagcttg aataacggaa cttgtccaat
     4021 catcagcgcg agtagcgcaa ctacaaccag cactgccagt aagatactca caacgatatt
     4081 cagcacgagt gatgctaatg agagccggtg aaagtagacg atcatcagcg gcagtagcat
     4141 cagttgcaca ctcgccgaca ccacgacagc gccaaacgtg taacgcaaca ccggctgcac
     4201 gtaataacgc tccagtcgta acgcgaccgg cgctttgaac agatggtagt catgcgttga
     4261 gcgcgcgatt tcctgcgccc accgtttctc gcgccagaac agcacctcgc aaatgtattt
     4321 gaatccacgc gcgcacatgg gcgggtaggg actatagcgc gtcggatacc atgcgccgat
     4381 ggccgacatg ttcgacaaca acggccaggc gatcacgagg atcgcgagca cagacagaaa
     4441 cgttagctga aatgacggat cgaagagctc tttcggacgg tgaaccagca agaccagcgc
     4501 tgccccgccg agggcattca gcgaactcga ttgtcgaaac agaatcgtag ccacacctgc
     4561 aaacgtgaac atcaacgctg cgcgcaccac tgacgcgtct gcaccgaccg cgaacgagta
     4621 cgcccaaacg atcagtgccg gaagcacgaa ttgcagcagc ctgcgcctgg tcagacgctt
     4681 cacgatcaaa aacacaactg cgccgatgaa gctgatatgc aagccgctga tgacgagaac
     4741 gtggaatgtg ccgccctcac gaaatcgttc cgacgccgtg gtcgaaaggt tgtagcgatt
     4801 gccgagcaac gtcgcgtcga ggacccctgc ggtttccggt tggaaactcg tatcaatttg
     4861 ttgttgcaat ctcgaccgcc agtcgtagag ccacgcgagt ggtggaaata cacgcgtgtc
     4921 gccgagacgt gtgatcgcag tgggactctt gattagcccg ctagcgtcat aaccgttgcg
     4981 atctagaaat tccgcgagtg atgaaacgcc ggggttgcgg taattgccgt cgcgatcgag
     5041 tgttgttatt acctgaatgc gtgtgccgta acggagttgt aaagctctga attcgtttat
     5101 gtcacgaagg gttacgagta acgacacacg gcctgccacg ttctcaacac gcaacgaaag
     5161 atacacacgg tcacgcgcaa actctggtgg tccatcaagc cagccggtaa gcgtgaacgg
     5221 ctcgccttcg gactgctcaa caagattctt gagcgcacgc gcttggtcta cgcgtcgctc
     5281 cagctcggcc agcgtgacgc cagtgaagaa cagtgctgat aacaatgaca gcaccgccaa
     5341 ccgaagtcgc tgcctgacga acagaatcag cattacggtt gtagacgcta ttccggcggc
     5401 taatgaaaat cttaacgaaa gataactcgc tgtgcagatg ccggcggaga atgctaccgc
     5461 gaggtgaacg agcggatgct gagtgaacag attgctgtga ggacgcattg ttgtgagtaa
     5521 gttttagaca ggatttacaa gattttacaa gattacatcc tgtcaatctt gagacatctt
     5581 gtaaatcctg tctaactata ccgtaagtcg cacgaccgtc tccacatgat gagtctgcgg
     5641 aaacagatca aaccctcgga ttgaactgat cgtgtagccg ccggcaacaa gcttccttag
     5701 atcgcgagca agcgtcgccg gatcgcatga cacataagaa atctgcggcg gatgaagatc
     5761 caaaatgccc ttaatcacgg ctgattccgc acctgcacgc ggtggatcga gcagaacaaa
     5821 tttcacctca cgcaccggtg cacttctgaa ccagtcagcc acaccggcgg cgatcacgcg
     5881 cgcattagca agtctcgcat cctgcaagtt tcgacgcgca aaacgcaccg cgaccggatt
     5941 cgcttccaca gcggtcactc gctggaattg ccgcgataac ggcaacgtaa acagcccgac
     6001 g
//
LOCUS       DASSFQ010000106        22469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1234806, whole genome shotgun sequence.
ACCESSION   DASSFQ010000106 DASSFQ010000000
VERSION     DASSFQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22469
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234806"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1484)
                     /gene="glnA"
                     /locus_tag="VFH96_03460"
     CDS             complement(60..1484)
                     /gene="glnA"
                     /locus_tag="VFH96_03460"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET6973053.1"
                     /translation="MNPKTVLKYAADQGAKFVSVRFTDLPGSWQHLTFPIAELGEDQF
                     EDGFGFDASSIRGWAAIHESDMLLVPDASRFWMDPFAEQPTLCLIGSAVDPITKEGYA
                     FDPRSVAQRAESYLKFTGLADTAYFGPEAEFFVFDQVKFHNDQHSAGFTVDSDEAHWN
                     SGRDANEFAGTNHGYRIRPKEGYVPCAPTDSLTDLRSEISLTLGKCGVTVECHHHEVA
                     TAGQCEIDFRYSTMLGTADNLQIFKYVVRNTAHQFGKTATFMPKPLYGDNGSGMHCHQ
                     SLWKSEKPLFAGDGYAGLSDTARYYIGGLLKHAAAIVAFAAPTTNSYKRLVPGFEAPV
                     NLAYSARNRSAAIRIPMFSTNPKLKRLEFRPPDPSCNAYLAFSAMLLAGLDGVQNRID
                     PGEPLEKDIYDLSPEELKDVPTLPGSLDDSLKALEADHDFLLKGDVFSPQLIDRWITY
                     KRDREITPLRMRPHPLEFSLYYDI"
     gene            1906..3843
                     /gene="speA"
                     /locus_tag="VFH96_03465"
     CDS             1906..3843
                     /gene="speA"
                     /locus_tag="VFH96_03465"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET6973054.1"
                     /translation="MTTAIEQTLETYGIENWAAGYFDVNRKGNLIVRPVEGDPRSADL
                     KEIIDDLAKRGITTPVLLRFPQLVAAQVRKLQRAFSRSIREFEYQGAHMCVYPMKVNQ
                     QRSVVEEYLREGSRYDFGIEAGSKAELYAALALEQPSDSLLVLNGFKDQEFIQLAFAG
                     AKVGKKVVIVIEKLNELDHVLNVVEHHEGALPMLGMRVKLYSKGSGRWEKSGGEAAKF
                     GLTTTELLEVIRRLEEADRMDMLKLLHFHIGSQLTDIKRIKNAMKEAARVYAKVYQMK
                     VPIELLDVGGGMAVDYDGSKTAFDSSANYTAQEFANDVIYTIKQVCDDENVPHPTIIQ
                     ESGRFLAAYHAILVTNVLDEIETVVEDITPIEIGEDDPQVVIELGDLRESISVKNYRE
                     YYHDALEHREELFTLFDLGLISLEDRAKGEVLFWDVCERADKYAQQAKYVSEEFGDLR
                     RLLCAKYLTNFSVFRSVPDHWALDQLFPIVPIHHLNKAPTEYATLCDITCDSDGIVDK
                     FVDLHDVKQVLELHHLSPEEPYYLAFLLVGAYQEVMGNNHNLFGAPHEAHIHIDEEGF
                     LIKKVIRGTTVGAAADRARYDRGFLHDGFRRLINRRVKDSELSEAEGYELAQFYESRY
                     EAYTYLSVNGVKSRSRRKTDY"
     gene            3905..4963
                     /locus_tag="VFH96_03470"
     CDS             3905..4963
                     /locus_tag="VFH96_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET6973055.1"
                     /translation="MVTQRKPKASKYLTTPTRPVQVDRDRSVAGLLEKMEGTGFGGRQ
                     LAEAHRIWLDMLGDNTTIMVAASGALIPAGMRRLLAYVIKNRFVDVLVLSGSLLVHDL
                     HETLGRYHFQAHPSMTDAELESAQISRMWDLLASDEEYREAKEWIGGFTNQLDQTRAY
                     SVREFLHLLGRELAEIASEDGVLTSAYKAKVPIFCPAIADSGLAVGIAGARFEKKNAF
                     LFDTIQDVLDMMNVMVNARVSGILNLGGGTTKSFIQQATLSSFLQKEHHHGHKYAIQI
                     STDAPHPGGRSNAVSFDEGLIYGKLARGAHTAYVPCDATIALPILITSLSQTAAKYMK
                     GRKKPNFTFGRELIVEAP"
     gene            complement(5043..6230)
                     /locus_tag="VFH96_03475"
     CDS             complement(5043..6230)
                     /locus_tag="VFH96_03475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6973056.1"
                     /translation="MDSNVGLAIQCVGILLITLLSLFMQSSIKSNALRYWTASWVSLS
                     LSLISLFAAFRLASTHTIFYSCYFLAEYLFGLLFIAGCRSQVTGKSLSRNHSYFLIPA
                     IVVSVVLPYVSRDFNDLFMIHAAVLATLFMIAFFTLRKSGERSPGIQLMLVALLLMTL
                     DFLHYVPAFGARKGLLGITVPATYFRYTSVFDLLFEILLGFGTVMVLMEGVRREVELT
                     NRKLTDARDQLELLVQMDPLTEALNRHAFHSLLRRPESGTGNTATSGSVAVLDIDNLK
                     PINDTHGHTVGDKAIRAVARAMRSLVRADDMLFRWGGDEFLVLMFKLPQPDAARRLEK
                     LNKILDENCERWIGEPVKVTVSFGVAGFTSLTDLGQAIEQADKAMYAQRNRVRGVPQK
                     AQI"
     gene            complement(6405..7202)
                     /locus_tag="VFH96_03480"
     CDS             complement(6405..7202)
                     /locus_tag="VFH96_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973057.1"
                     /translation="MSAASPAVETFQLVKQFSDFVAVDHINLRVNRGSFFGFLGPNGA
                     GKSTTIKMLTGLLAPTSGTLRVLDLDISKQPLEVKRLIGVVPEDLNLFERLTGAEMLS
                     FTGRMYGLRAEEIAQRSKELLELMELQDEPRKLIIEYSHGMKKKLSLACALIHRPEIL
                     FLDEPFEGVDAIASRTLKDLLSRLTARGLTVFLTSHVLEIVERLCSDIAIIAQGKLLA
                     AGSLNELRKGIRVEGDGDGPVSLEEYFIHVVGGTRSSNEEEVLQWLT"
     gene            complement(7227..8402)
                     /locus_tag="VFH96_03485"
     CDS             complement(7227..8402)
                     /locus_tag="VFH96_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6973058.1"
                     /translation="MKGQTRPRRLLLCLDGVPHKLIEGERSRGLFDSFKSPARLLSPF
                     PTMTNVALSAMLGATPPAGYESLYFDRKARELRGGISKYLGRRTPDKVPSSYMEELDY
                     QEPLPFEFLIYVAPEKVWRADMQRFRERFRAAPQNRDYFAFLKATDGLLHAQGPQRLA
                     VALESLDKILKEIRDYCGDETEIVMFSDHGMNLEENRRIPLASTLRRRGFKVVIPAFG
                     LCSYAALYCDEDVIPEVARTSAEVAGVDFAVYKDAAAVVIESSRGQARVEYEAATDSY
                     RYVIVSGDPLQLGSFPNTFATDATWFERTAGHRYPNAVANVYKSLFTPRVKHTADVLI
                     SMNDGYYYGMTAFGRFVRLRATHGNALQSSSNAFLMSTHRNFPAFVRADDAETLLRN"
     gene            complement(8438..9697)
                     /locus_tag="VFH96_03490"
     CDS             complement(8438..9697)
                     /locus_tag="VFH96_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973059.1"
                     /translation="MKKLAVVVVMLLGAVTVFAQQPPWLTDAERSRFEELRRSGLDAL
                     YNIDYDKANRDFNEIIKLYPNHPGGYQLLAARLWIKTLYESRRLQVSLYSSESFYTNG
                     DDKVDPKIVTEFRNLTREAKRLAEAKLKQEPKNIEALDFLANTEGLKASFEEAVERRH
                     FAALRDGNDAVDHHREVLKLDPKYIDAQITIGLYEYVVGSLPLPVKILAGATGYRGSK
                     KRGLAMLEQVAKEGRWSQDDAKTVLILLYTREKRFADALVFTRELSVKYPRNYLLRLE
                     TADALVSLAGVKRKEKDLTGAVQAEKEAFATFDELLRDRNTAARALDLIHFKYGEALL
                     IAGYHDRAAKEFLAATKVDRGEPGLITMAHLYAARSYDVAGKRDEALAQYKEVLARPN
                     VYDAHDEAKRGLRQAYKADQSKPDAEQ"
     gene            complement(9731..10909)
                     /locus_tag="VFH96_03495"
     CDS             complement(9731..10909)
                     /locus_tag="VFH96_03495"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6973060.1"
                     /translation="MNVTHLECGLCGTRHEAHRLHNLCTACGKPLLVRYDLERAKMFM
                     KKESLAARRPDMWRYREMLPVERDEDIVSLGEGWTPLLRAVRLGRRVGVPDLFIKDES
                     QNPTQSFKARGMAAAVSMAKELGVKKLAVPSAGNAAGALAAYAARAGLKSYIFMPRDT
                     PRANVVECQQTGAHVTLMDGLITDCGAEVGRRKEKEGWFDVSTLKEPYRIEGKKTLGY
                     ELAEQMEWRLPDVIIYPTGGGTGLIGMWKAFDEMEQLGWIGSKRPRMVTVQAEGCAPI
                     VDAFEEGNRFADEFPDAATVASGLRVPKAIGDFLILDALRASGGTAVAVSDEELIEAV
                     RDIGAVEGVFCAPEGAACLPALRKLVESGEVNKHERVVLFNTGSGVKYMEAFTADQTD
                     "
     gene            complement(11099..11620)
                     /locus_tag="VFH96_03500"
     CDS             complement(11099..11620)
                     /locus_tag="VFH96_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET6973061.1"
                     /translation="MIALVSAGSIAFAQGFKRISEILSGYEETPAPISTTGNGSFNAR
                     ISNDESRIDWELSYSDLEGAVQQSHIHFGTKGITGGITVFLCTNLGNGPAGTQPCPAP
                     PATISGTIVAADVSPNIPATAAARAQGLNTGEMDELIKAIRAGATYVNVHSTTWPGGE
                     IRSQIDGNAGHDH"
     gene            11795..12676
                     /locus_tag="VFH96_03505"
     CDS             11795..12676
                     /locus_tag="VFH96_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET6973062.1"
                     /translation="MPISLLALDLDGTLLDARGIISERNRIAIDHARRQGVHVALVTG
                     RRFRDSRPVALELGLDVPLISHNGALTKHAATLQTVSVLPLPLDAAHASLKIGRKADA
                     DALLSDDHEGLGVLVYDHIRGGNTAAHKYISWASRIHGDEEGTNAVQQVASLEDYLDH
                     DPIHLSFSGGCAEMDRLEETLKSELGATVKVLGTKYLEQDFTLLDIVNAAASKGAGVA
                     AAAAELGVMREEIMAIGDNFNDLEMLLFAGTGVVMANAPLLLREIAGLHPTASNRDDG
                     VALAIEQFILNPQLINQ"
     gene            12686..13165
                     /locus_tag="VFH96_03510"
     CDS             12686..13165
                     /locus_tag="VFH96_03510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6973063.1"
                     /translation="MANRTAVIQTNKGTIKVELLETDAPKTTENFITLAERGYYDGII
                     FHRVIKNFMIQGGDPTGTGRGGESAWGGRFNDEIDAKSPVYQRGYKAGTMAMANAGPN
                     TNGSQFFIMHVDYPLPPSYTIFGKVIEGQDVVNAIATSQTMNDKPVSEVKMEKVTIE"
     gene            complement(13226..14011)
                     /gene="thiD"
                     /locus_tag="VFH96_03515"
     CDS             complement(13226..14011)
                     /gene="thiD"
                     /locus_tag="VFH96_03515"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET6973064.1"
                     /translation="MTRPVVLSIAGLDPSGGAGLVADIKTIAALGCFPAAALTSITFQ
                     NTTGVFGAEHQSAATLRAQVEPIVQDLTVAAAKTGMLPTAEIVAEVARLFAEENLPAP
                     VVDPVVVATSGDVLIDDEAFEILKTKLFPLARVVTPNIPEAEKLAGLSIKSETDMRRA
                     AEAIQSIGARSVLVKGGHRSVGGQALDILLDENGIFTEFQTDYIEIGDVHGSGCTLSA
                     AIAANLAKDLTLEQAVAAAKKYVTDAIRALKTAPRIGHGAKPL"
     gene            complement(14008..14616)
                     /gene="thiE"
                     /locus_tag="VFH96_03520"
     CDS             complement(14008..14616)
                     /gene="thiE"
                     /locus_tag="VFH96_03520"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET6973065.1"
                     /translation="MSLPVPIIYPITSGKTSPHDFSEILQFLRAAVDAEVPLFQIREK
                     SLPARVLYELVARATEITHGSRTRLLVNDRSDIARAAGADGVHLTTQSLPVEVVRNIC
                     GPEFLIGVSTHSLEEARAARAGGADFVVFGPVFETESKRAYGEPQGLSKLAEVARELG
                     EFPVVAIGGITLDNADECFHAGARGIAAIRMLNDIENLTRWK"
     gene            complement(14573..14932)
                     /locus_tag="VFH96_03525"
     CDS             complement(14573..14932)
                     /locus_tag="VFH96_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973066.1"
                     /translation="MSAKLSVIFYIILCLEIGIFLTVAPWWPHGMWGISDWGNNYFLL
                     YAARKTGIQGLQAVVASGWVRGAVSGVGLLNLGIAFWEIFNFKRTVTALQNETSARKS
                     STEDVASSSDHLSNNER"
     gene            complement(15004..17268)
                     /locus_tag="VFH96_03530"
     CDS             complement(15004..17268)
                     /locus_tag="VFH96_03530"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973067.1"
                     /translation="MAFDKAKVLKAAEKFLSQGKFNAAIKEYRQIVDNDADDLTTLNM
                     LGDLYVRSNKKEEAIQCFERIAEHYSAQEFNLKAIAMYKKIERLRPRDPVVALKLADL
                     YANQGLVHDARAQYLVVAEAYTKSGDNKRSLDILHKIADLDPNNTEIRLKLADGYLKE
                     NMRRESAAAFVQAANRFHQIGSHDQALDAYNKALQLVRDDREAIRGMLESHIARGTAD
                     EAAEVLDRIVETNEDDKELVSMLGRAYLEAEDPKGAERATSLLMAQDATTYPQFLPVT
                     RLYLKTGEVDEVIRILSTIIERMLAGREERELLEIVNQVLEQNPDHVAGLRMLVRIHW
                     WQRDMDALRSSLERLAESAEASELVDEERYALTQLVRLAPDEQRYLDRLNLLGGLQEE
                     TPEEFAAIPEPESGETDVPQFETFAVVEDEDEAGDVAPVISDEFETNAAPDSTFSDPT
                     ASFADLNEDESFATSATDFQEVDFSIVSTPETPVAAAEEAPVAEERHENVMRQELESV
                     DFYIAQGYSDIAIDTLEMLERQFGQHPEIQSRREKLTQRDQPVEEATVFEFGGVEEVA
                     TATPVPAESITFDADSAYASLVDDGNNVGAAKPVAMGIDAGLAELFEEFRAAEEGDEV
                     REDYETHYNMGTAYKEMDLMDEAIQEFQTAASLVKPGDNTSRYLQCCNMLGHCFIQKG
                     MPEAAVLWFKKGLQAPGHSEDEYQALRYELASAYEQLGDLKQARDFYTEVYGVDVSYR
                     EVAEKLSQLRQKQD"
     gene            17441..18904
                     /locus_tag="VFH96_03535"
     CDS             17441..18904
                     /locus_tag="VFH96_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6973068.1"
                     /translation="MITTATNGKTLRGKAGQQIALVGLGIYVVFAPHSVAASVIGVAL
                     AGLGWVIRTLQTRSLGLRWSKFDLIIVLSLLWTFASALLSEEPHISVAKLQASWCVFI
                     FYLARATLTRRSALILVALLIVSASAGALYSAFDLLRGRGVVVESIAPGSPFNQIGIR
                     PGDTIWQINRRRVYSTTEIDQILAQVPANTAVTAGLITQGEQVERSFTLAQPALSGVI
                     GSESNHRFRASGWTRHYETFAELIQMIAQLALGFALAHLRNHGPNKIFRIAIAAAALL
                     TAALVFTAMRTVIIAFVIGASVMALRSLRATMRVAFTFALFAVLAFAAVVVWQTRDRN
                     ALLLGDPSSSLRYEVARNGLSRILIHPIFGHGMDAMQLHWNEWGFPGKDMLHLHSTPL
                     QLAFDRGLPMLALWLWMMILFWLTIARAEKRAADLSDTNSYGILLGTLGALTGFLMSS
                     LVNYNYGDAEVAMMFWFLMGIGLIGPIGHIGPITSSD"
     gene            complement(18906..19463)
                     /locus_tag="VFH96_03540"
     CDS             complement(18906..19463)
                     /locus_tag="VFH96_03540"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6973069.1"
                     /translation="MKTNNLVSKWAIGIFAMLLLAVAPGVALAQQRASNPQLERKVRK
                     ELVTLPYYGVFDNLAYKVDGGTVTLYGEVVRPSTRSDAAGRVKRLAGVTRVVNNIRVL
                     PLSSFDDNIRRATYRSIARMGGLYRYLQGTNPSLHIVVDNGHVTLEGVVSGSGDRNLA
                     YMAANRVSGVFSVKNNLRVEGEGAQ"
     gene            19563..20072
                     /locus_tag="VFH96_03545"
     CDS             19563..20072
                     /locus_tag="VFH96_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6973070.1"
                     /translation="MTRPQTTEAADYYFRYIDLVTSDDIVPAIEKQMGEMLQFLSGIN
                     EEQSLHSYAPGKWTIREVLNHVNDGERVFAGRGFWFARGFNDALPSFEQDIAVQNAHA
                     NNTSWADLVEEFKNVRLATISLFKSLPEEAWDRTGVASDNPVSVRAIAYIIAGHVAHH
                     IGVLREKYL"
     gene            20247..20777
                     /locus_tag="VFH96_03550"
     CDS             20247..20777
                     /locus_tag="VFH96_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973071.1"
                     /translation="MKRCPKCEFIYEDDQSLCDMDGVLLVLDSRKLPNHGALQTVPAA
                     KTAPKRNRAVPAFATVILALVLGLVYYVSVHRKATQNNYTPPPPSAASTSNPVPAEVN
                     VNPTPEAQPPAAEEPKPEPAKPTAAAAAPAPVKKVATKPTPTPDKPKVEEKKDESKVN
                     SILKKTGRILKKPFKF"
     gene            20796..21368
                     /locus_tag="VFH96_03555"
     CDS             20796..21368
                     /locus_tag="VFH96_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973072.1"
                     /translation="MKRCPQCLFLFPDSDSKCDFDQTPLEHVDDSAIDGAIRKPKRRA
                     LPIAAAIGLLLGVLIIAIYYGLTRQQQKASAATETPVTTVEPVAAPAATPSPSVEVSP
                     SPSPSIAPSPRSSPEKLSAAHTRATSDPVSTSGPGMGNRQGGKPVIMLTSGGKIEADE
                     VWRTKDGVWYRRNGMVTLLKRSQVKTIVTR"
     gene            21379..21858
                     /locus_tag="VFH96_03560"
     CDS             21379..21858
                     /locus_tag="VFH96_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973073.1"
                     /translation="MKASASLLVLVLVLGVAISCKLSERLGGDKNAGTVSELWPDVPP
                     FQGATKAEVEIPLGVRLLIRGMSQGRVNFISFRTDKTAQEVKDFYSNDRMKAAGWTAN
                     DKGCTGDMEDKKNHGAMCMFSRRDGGKEEALAIVIAQDEKLPDTNIVYARIDTTKTK"
     gene            21995..>22469
                     /locus_tag="VFH96_03565"
     CDS             21995..>22469
                     /locus_tag="VFH96_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973074.1"
                     /translation="MTCRRIVLLALTIALALASVLVMNPGKVNSMDAQQLPNNLPHPN
                     PGGKAATFSTQGNVDLTGEFFQAQGTNGRSCASCHIAEEAWSINPGTLQDLFDETGGT
                     HPVFNLLDANNPNMDVSTPAARLSAYSMMLSHGVFRRGGAPRPNSDWELIAVEDPH"
BASE COUNT         5101 a         6434 c         5985 g         4949 t
ORIGIN      
        1 tttctctttg cgttgcgccg ctgcgccgct gcgtgaggaa tccaccctac agttgctcct
       61 taaatgtcgt aatacagcga gaactccagc gggtgcgggc gcatgcgcag cggcgtgatc
      121 tcgcgatcgc gcttgtaagt gatccagcga tcgatcagtt gcggactgaa cacgtcgccc
      181 ttcaacagaa agtcgtggtc ggcttcgagc gccttgagcg aatcgtcgag cgatcccggc
      241 aaggtcggca cgtccttcaa ctcttccggt gaaagatcgt agatgtcttt ctcgagcggc
      301 tcacccggat cgatccggtt ctgaactcca tcgagtcccg ccagcagcat cgccgaaaac
      361 gcgagatacg cattgcacga cggatccggt ggacgaaact ccagtcgctt caacttcgga
      421 ttcgtcgaga acatcggaat tcgaatcgcc gccgaacgat tgcgcgcgga gtacgcgaga
      481 ttcaccggag cttcaaagcc aggcacgaga cgtttgtagc tgttcgtcgt cggcgcggca
      541 aacgcgacaa tcgccgccgc gtgcttcaac aagccaccga tgtaatagcg agcggtgtcg
      601 gaaagtcccg cgtaaccatc gccggcaaag agcggcttct cgctcttcca caacgactgg
      661 tgacagtgca ttcccgaacc gttatcgcca taaagcggct tcggcataaa cgtcgcggtc
      721 tttccaaact ggtgagcagt attgcgaact acatatttga atatctgaag attgtctgcc
      781 gtccccaaca ttgtcgagta gcgaaaatcg atttcacatt gtccggcggt agcaacttcg
      841 tggtgatgac actcgacagt cacgccacac ttgcccaacg tcagcgagat ttcagatcgc
      901 aaatccgtca gcgaatctgt cggcgcacac ggcacgtaac cttccttcgg gcgaatgcga
      961 taaccgtgat ttgttccggc aaactcgttc gcatcgcggc cgctgttcca atgtgcttcg
     1021 tctgaatcga ccgtgaatcc ggctgagtgc tggtcgttgt gaaacttcac ctggtcgaac
     1081 acgaaaaact cagcttcggg accgaagtaa gcggtgtcgg cgagtccggt gaatttcaga
     1141 taactttcgg cgcgctgtgc gaccgaacgt ggatcgaagg cgtatccttc cttcgtgatc
     1201 ggatcgacgg cactgccgat caagcagagc gttggttgtt cggcgaatgg atccatccaa
     1261 aagcgggacg cgtcggggac gagtagcatg tctgactcgt gaatggcggc ccagccacga
     1321 atcgaagacg catcgaagcc gaaaccatct tcgaattggt cttctccgag ttcagcaatc
     1381 gggaaggtca ggtgttgcca ggagccgggc aaatccgtga agcgaacgga aacgaatttg
     1441 gcgccctggt cggcagcgta tttcaagaca gtcttagggt tcattttctc tcctgaacaa
     1501 agattaaggg agtgttgggg gtgctcttcg cccctgtgcg gtaattggtg cgaagtaatt
     1561 tggcgtgctt aacgacttta ggaaaacagt cgtacggcat tgaaggcgtc gcgtaaaaaa
     1621 ctgtgattat aatcgcgggc tgaatagtgt caaggggaaa cgcgtggtat aggtcgaata
     1681 ggacctgtat gacctataaa aaccctaatg ttttgaagta gattggagca tttgttgaaa
     1741 caaaaaagtt atagaccgca acggccattt tctgttacat tccccgacta aagcgttcca
     1801 cccaaacaaa aaactccgca actcgattcg gaaaatgtac atctgcgttt cagctaaagg
     1861 gattcatatc gttcacgttt tatttttgag gcaggagacc gccaattgac cacggcaatc
     1921 gagcaaacgc tcgaaactta tggaatcgaa aactgggccg ctggctactt tgatgtaaac
     1981 cggaagggaa accttatcgt ccgccccgtc gagggagatc ctcggtccgc ggacctcaaa
     2041 gagatcatcg atgacctcgc caaacgtggc atcacgacgc ccgtgctgct gcgtttcccg
     2101 caattggtcg cagctcaagt aagaaaactt cagcgcgcgt ttagccgctc tatccgcgag
     2161 ttcgaatacc agggcgcgca catgtgtgtt tacccgatga aggtaaacca gcagcgctcg
     2221 gtcgtcgagg agtacctgcg cgaaggctcg cgctacgact tcggcatcga agccggctcc
     2281 aaagccgagc tctacgctgc actcgcgctc gaacaaccga gtgacagcct gctggtgctc
     2341 aacggtttca aggaccagga gtttatccag ctggcgttcg cgggcgcgaa agtcggaaag
     2401 aaagtcgtga tcgtgatcga aaagctcaac gagctcgatc acgtgctcaa cgtcgtcgag
     2461 catcacgaag gcgcactgcc gatgctcggt atgcgcgtca agctctattc gaaaggctca
     2521 ggccgctggg aaaagtcggg tggcgaggcc gcaaagtttg gactaacgac gaccgagctg
     2581 ctggaagtga tccgccgtct cgaagaagcc gatcgcatgg acatgttgaa gctgcttcac
     2641 ttccacatcg gctcacaact caccgatatc aagcgcatca agaatgcgat gaaggaagcg
     2701 gcgcgcgtct acgcgaaggt ctaccagatg aaagtgccga tcgagctgct cgacgtgggc
     2761 gggggtatgg ctgtcgacta cgacggctcg aagaccgcgt tcgactcttc cgccaactac
     2821 accgcgcagg agtttgccaa cgacgtcatc tacacgatca agcaggtgtg cgacgacgag
     2881 aacgttccac atccgacgat catccaggag tcgggacgct tccttgccgc ttatcacgcg
     2941 attctcgtga cgaacgtgct cgacgagatc gaaacagtcg ttgaagacat cactccgatc
     3001 gagatcggtg aagacgatcc gcaggtagtg atcgagctcg gcgatctgcg cgagtcgatc
     3061 agcgtgaaga actatcgtga gtattatcac gacgcgctcg aacatcgcga agaattgttc
     3121 acgctcttcg atctcgggtt gatctcgctc gaagatcgtg cgaaaggcga agtgcttttc
     3181 tgggacgttt gcgagcgcgc tgacaagtac gcgcaacagg caaaatacgt gtcagaagag
     3241 tttggcgacc tgcgccggct gctctgcgcc aaatacttaa ccaacttttc tgtgtttcgt
     3301 tcggtgcccg atcactgggc gctcgatcaa ctgtttccga tcgttccgat ccatcatctc
     3361 aataaagctc cgactgaata cgcgacgctc tgcgatatca cctgcgactc agatggtatc
     3421 gtcgacaagt ttgtagacct gcacgacgtc aagcaggtgc tcgagttgca ccacctcagt
     3481 cctgaagagc cttactacct cgccttcctg ctggtcggtg cgtatcagga agtgatggga
     3541 aacaatcaca atctcttcgg cgcgccgcac gaggcacaca tacacatcga cgaagaagga
     3601 ttcctgatca agaaggtgat caggggaacg actgtcggcg ctgcggccga ccgtgcccgt
     3661 tacgaccgcg gttttttgca cgacggtttt cgacgtctca tcaaccggcg cgtcaaggat
     3721 agcgagctga gcgaagccga gggctacgag ttggcccagt tctacgagtc acgttacgag
     3781 gcttatacgt atctctctgt gaacggtgtg aagtcgcgtt cacggcggaa gactgattac
     3841 tgattatgta atacctgaaa tctgatctaa tcgaacgttc aatccaagag gtttgacaac
     3901 ctgcatggtt actcaacgaa aaccaaaagc atcgaagtac ctgaccactc ccacgcgtcc
     3961 agtccaggtg gaccgcgatc gttctgtggc gggacttctc gagaagatgg aaggaacagg
     4021 ctttggaggc cgccaacttg ccgaagcgca tcgcatctgg ctcgacatgc ttggcgataa
     4081 cacaaccatt atggtcgctg cttcaggggc tctcattccc gcgggcatgc gccgcttgct
     4141 ggcgtatgtg atcaagaacc gcttcgttga tgtcctcgtt ctctcgggtt cactcctcgt
     4201 ccacgatcta cacgaaacac tcgggcgtta tcacttccag gcccatccca gcatgactga
     4261 cgctgagctt gaatcagccc agatcagtcg catgtgggac ctgctcgcga gcgacgagga
     4321 gtatcgcgaa gcgaaggaat ggattggcgg gtttactaat caactcgatc aaacgcgggc
     4381 gtactcggtg cgtgagttct tgcatcttct gggacgtgag ctggcggaga tcgcgtcgga
     4441 agatggggtg ttgacgtcgg cgtacaaggc gaaggtgccg atcttctgtc cggcgattgc
     4501 tgatagtggg ttggcggtcg ggattgcggg tgcgcgtttc gagaagaaga atgcttttct
     4561 cttcgacacg attcaggacg tgctcgacat gatgaacgtg atggtcaacg cgcgcgtctc
     4621 gggaattctc aatctcggtg gcgggacgac gaagagtttc attcaacagg cgacgctctc
     4681 gtcgttcctg cagaaggaac atcatcacgg gcacaagtac gcgattcaga tctcgaccga
     4741 cgctccacat ccgggtggta gatcgaatgc ggtgtcgttt gatgagggat tgatttacgg
     4801 aaaactcgcc cgcggcgcgc acaccgctta cgttccctgc gacgccacga tcgcattgcc
     4861 gatactgatc acgtcgttgt cacaaaccgc agcgaagtac atgaagggcc gtaagaaacc
     4921 aaatttcacc ttcggtcgcg agctgatcgt cgaagcaccg tagccacaaa aggcacaaaa
     4981 agcacaaaag aatttatgct ctcagagggc ataaattctt ttgctttttg tgccttttgt
     5041 ggctaaattt gtgccttttg tggcacccct ctgacgcgat tgcgttgcgc gtacattgct
     5101 ttatccgcct gttcgattgc ctgtcccaga tcggttaacg acgtaaaccc ggcgacgccg
     5161 aacgagaccg tcacctttac cggctctcca atccagcgct cgcagttctc atcgagtatc
     5221 ttgttcagct tctcgaggcg ccgtgcggcg tcaggctgtg gaagcttgaa catcaacacc
     5281 agaaactcat caccgcccca acggaacagc atatcgtccg cacgcacgag ggagcgcatc
     5341 gcccgcgcga ctgcacggat cgctttgtca ccgactgtat gaccatgcgt gtcgttgatc
     5401 ggcttcaggt tgtcgatgtc gagcaccgca acgcttcccg aagtcgcggt attgcctgtt
     5461 ccggattcag gccggcgcag gagggagtga aacgcgtgac gattcaatgc ttccgtgagc
     5521 ggatccatct ggaccaacag ctcgagctga tcgcgcgcat ctgttagctt tcggttcgtg
     5581 agttcgactt cgcggcgcac accttccatc aacaccatca ccgtcccgaa cccaagcagt
     5641 atttcaaaca gcagatcgaa gactgacgtg tagcgaaagt acgtcgccgg cacggtgatg
     5701 ccgagcagtc ctttgcgtgc tccaaacgcc ggcacgtagt gcagaaagtc gagcgtcatc
     5761 agcagcagcg ccaccaacat caactgaatt cccggactcc tttctccgct tttgcgcaga
     5821 gtaaagaacg cgatcatgaa cagcgtcgcc agtaccgcgg cgtgaatcat gaatagatcg
     5881 ttgaagtcgc gcgagacgta cggcaggaca accgacacaa cgatcgcggg tatcaggaag
     5941 taactgtggt tcctgcttaa cgatttgccg gtcacctggc tgcgacagcc ggcgatgaac
     6001 agcaaaccga agagatactc ggccaggaag tagcaggagt aaaaaatcgt gtgggttgaa
     6061 gcgagccgga acgcggcaaa gaggctaatc agcgacagcg ataggctcac ccatgaagca
     6121 gtccaatacc tgagcgcgtt gctcttgatc gaagactgca tgaacaatga aagcagcgta
     6181 atgaggagta tgccaacaca ttgaattgca agaccgacat tcgaatccat actggggacc
     6241 tcgtagggag aacgagttta atcaggaaag ctccgggcag tgaggctgat aggattctag
     6301 cccaccggag ggcttgaggg aaagactttt ttagccacag attacacgga taacacagat
     6361 aagcagttaa gccgtgttat ccgtgtaaat ccgtggcaaa atccttacgt cagccactga
     6421 agcacttctt cttcattcga gctgcgcgtg ccgccgacga cgtgaatgaa atactcctct
     6481 agcgacacag gaccatcgcc gtcgccctcg actcttattc ccttgcgcag ctcgttcagc
     6541 gatccggctg ccaataactt cccctgcgcg atgatggcga tgtcgctgca aaggcgctcg
     6601 acgatctcga gcacgtgcga ggtgagaaac accgtgaggc ctctcgccgt taagcgcgac
     6661 agcagatcct tcaacgtgcg tgaagcaatc gcgtcaacgc cttcgaacgg ctcatcgaga
     6721 aacagtatct ccggccggtg aatcaaagcg catgcgagcg agagcttctt cttcataccg
     6781 tgtgagtatt cgatgatcag cttgcgcggc tcgtcctgaa gctccatcaa ctcgagcagc
     6841 tctttcgagc gctgcgcgat ctcttcggcg cgcagcccat acatacgtcc ggtgaacgac
     6901 aacatctcgg cgcccgttag tcgctcaaac aagttgagat cctcaggcac aacaccgatg
     6961 agccgcttca cttccaacgg ctgcttgctg atatccagat cgagaactcg caacgtgccg
     7021 ctggtcggcg ccagcagtcc ggtgagcatc ttgatggtcg tcgatttgcc ggcgccgttg
     7081 gggccgagaa aaccgaagaa gctgccgcgg ttcacgcgca ggttgatgtg atcaactgca
     7141 acaaagtcac taaactgctt gaccagttga aacgtctcga ccgccggcga tgctgctgac
     7201 atgaatcaca gattaacgga tctggatcaa ttacgcagca gcgtctccgc atcgtctgca
     7261 cgaacgaagg ccgggaaatt tctatgagtg ctcatcagaa acgcattcga gctggactga
     7321 agcgcgttgc catgcgtcgc gcgcaagcgg acgaagcgtc cgaaggctgt catgccgtag
     7381 tagtaaccgt cgttcatgct gatgaggacg tcggcggtgt gcttcacgcg cggcgtaaac
     7441 agcgacttgt aaacattcgc gaccgcgttc ggataacgat gtccggcggt gcgctcaaac
     7501 cacgttgcgt cagtggcgaa cgtgttcggg aaagaaccca gttgcagtgg atcaccgctg
     7561 acgatgacat atcgataact gtcagtagcc gcttcgtatt caacccgcgc ttgtccacgc
     7621 gaactttcga taacgactgc tgccgcatcc ttgtagactg cgaaatcgac gcccgctact
     7681 tccgcactcg tgcgcgccac ttcaggaatc acatcctcgt cacaatagag cgccgcataa
     7741 ctacagagtc caaatgccgg tatcacgact ttgaagccgc gacgacgcag agttgaagcg
     7801 agcgggatgc gacggttctc ttcgagattc atgccgtgat cggaaaacat cacgatctct
     7861 gtctcgtcgc cgcaataatc gcgaatctct ttgaggattt tgtcgagtga ctcaagcgcg
     7921 actgccaaac gctgcggccc ctgcgcgtga agcaaaccat cagtggcttt cagaaacgcg
     7981 aagtaatcgc gattctgagg cgcagccctg aagcgctcgc gaaaccgttg catatctgca
     8041 cgccaaacct tctccggtgc gacgtagatc aaaaactcga acggcaacgg ttcctggtaa
     8101 tcgagttcct ccatgtacga cgacggcact ttatccggcg tacgacgacc gaggtacttg
     8161 ctgattccac cccgcagctc gcgtgctttg cgatcgaaat aaagactctc gtaacccgcg
     8221 ggcggcgtcg cgccgagcat cgccgacagc gccacattcg tcatcgtcgg aaatggagaa
     8281 agcagacgcg ccggcgactt aaaactatcg aacaatccac gactacgctc cccctcgatc
     8341 aacttatgag gaactccatc gagacaaagc aacagacgcc gtggacgtgt ttgacccttc
     8401 attaaaaaat ccgttttaag atccgtgtta tctgtggcta ctgttcagca tcaggcttcg
     8461 attggtcggc cttataagcc tgcctcaacc cgcgtttcgc ttcatcgtgc gcgtcataaa
     8521 cattcggacg cgcgagcact tccttgtatt gcgcgagagc ctcatcacgc ttcccggcaa
     8581 cgtcgtacga ccgcgccgca taaagatgcg ccatcgtgat caaccccggc tcaccacgat
     8641 cgaccttcgt cgccgccaga aactccttcg ccgcccgatc gtgatagcct gcaatcaaca
     8701 atgcctcgcc atacttgaaa tgaatcagat cgagcgcacg tgcagccgta ttgcgatcgc
     8761 gcagcagctc atcaaacgtc gcaaatgctt ccttctcagc ctgcactgca ccggtcagat
     8821 ctttttcttt ccgcttcact cctgcgaggg aaaccaacgc atcagcggtc tcgagccgca
     8881 ataaatagtt gcgcggatac ttcacactga gctcacgtgt gaaaacaagc gcgtcagcaa
     8941 aacgcttctc acgtgtgtaa aggagtatca acacagtctt cgcatcgtcc tgcgaccagc
     9001 gaccttcctt cgccacctgt tcgagcatcg cgagtccacg cttcttcgag ccgcgataac
     9061 ccgtcgcgcc cgcgagtatc ttcaccggca gcggcaacga accgacgacg tactcataaa
     9121 gaccgatggt gatctgcgcg tcgatgtatt tcggatcgag cttcaacact tcgcgatgat
     9181 ggtcaacagc gtcattgcca tcacgcagcg ccgcgaagtg tcgacgctcg acagcttctt
     9241 cgaacgacgc tttcagtcct tccgtgttag caaggaaatc gagcgcctcg atgtttttgg
     9301 gttcctgttt gagctttgct tcggcgagac gtttcgcttc acgcgtgaga ttgcgaaact
     9361 cagttacgat cttcggatcg accttgtcat cgccattcgt ataaaacgat tccgagctgt
     9421 aaagcgacac ctgcaagcgt cgcgactcat aaagcgtctt gatccagagc cgcgccgcca
     9481 gcagttgata accccccgga tgattcgggt aaagctttat gatctcgttg aagtcgcgat
     9541 ttgccttgtc gtagtcgatg ttgtagagcg cgtcaagccc actgcgcctt aactcctcaa
     9601 accgcgaccg ctccgcatcc gtcaaccacg gcggctgttg agcaaatacg gtcaccgcac
     9661 cgaggagcat aaccaccaca acagcaagtt tcttcaacgc cacgtccctc ccaaccaaaa
     9721 atccgcgttt tcaatccgtc tgatccgcgg taaacgcttc catatatttc acgcccgatc
     9781 cggtattaaa aagcacgacc cgttcatgct tattaacctc acccgactca accaacttcc
     9841 tcagtgccgg caaacacgcc gcaccttccg gcgcacaaaa cactccttca accgcaccaa
     9901 tgtcacgcac cgcctcgatc aactcttcat cactcacggc caccgcagtc ccacctgaag
     9961 cacgcaacgc atcgagtatc aaaaagtctc cgatcgcctt cggcacgcgc aaacctgacg
    10021 ctaccgtggc cgcatccgga aactcatccg caaagcgatt tccttcttca aacgcgtcca
    10081 cgatcggcgc acaaccttca gcctgcaccg tcaccatgcg cggccgttta cttccgatcc
    10141 aacccagttg ctccatctcg tcgaacgctt tccacatgcc gatcaaaccg gtaccgccgc
    10201 ccgtcgggta aataatcacg tcgggcaagc gccactccat ttgctctgcg agctcgtatc
    10261 caagcgtctt ctttccttcg atgcgatagg gttctttcaa tgtcgagacg tcgaaccaac
    10321 cttccttttc ctttcgcctt ccgacctcag caccgcagtc agtgatcaag ccatccatca
    10381 acgtcacgtg agcgccagtt tgctgacact cgaccacatt tgcgcgtggt gtatcgcgcg
    10441 gcatgaagat gtacgacttg agtcccgcgc gagcagcgta ggccgccagt gcgccggcag
    10501 catttccggc ggatggaact gccagtttct tgacgccgag ttctttcgcc atcgagacag
    10561 cggcggccat tccgcgtgct ttgaaactct gcgttggatt ttgcgattca tctttaatga
    10621 aaaggtccgg gactccgacg cgcctgccca atcgaacggc gcgcaaaagt ggcgtccaac
    10681 cttcccccaa cgaaacgatg tcttcgtcgc gttcgaccgg gagcatttcg cgatagcgcc
    10741 acatatcagg gcgccgggcc gcgagcgact ccttcttcat aaacattttg gcgcgctcta
    10801 aatcgtagcg aaccagcaaa ggcttaccgc aggcagtgca gaggttgtgc aaccgatgtg
    10861 cctcgtgtct tgtgccacag aggccacatt caagatgtgt gacattcatt gatagatttt
    10921 ccttaattac tgggcaaatt acgggagaac ggcgctaagt atgcgctaga aatgagaatt
    10981 cgaggtaacg cggtttgtta tttgaggtgc ggcggcgggc gcaggcaaga gcaagaggtt
    11041 tgcgctaggt gcagcaaacc ccttgcactc ggagcaagct aattagtttc ggcaaatctc
    11101 agtggtcgtg gccggcattg ccgtcaatct ggctgcggat ttcgccaccg ggccaggttg
    11161 tgctgtgaac gttgacgtat gtcgcaccgg cacggatggc tttgatcaac tcgtccatct
    11221 ctccggtatt caggccctgg gctctggctg ctgccgtcgc cggaatgttc ggactgacgt
    11281 ctgccgctac aatcgtgcct gagattgttg ccggaggcgc cggacagggc tgagttcctg
    11341 ccggaccatt gccgaggttg gtgcagagaa acacagtgat gccgcccgta atccccttag
    11401 ttccgaagtg gatatgggat tgctggaccg cgccttcgag gtctgaataa ctgagttccc
    11461 agtcgattcg cgattcatca ttgctgatgc gcgcgttgaa gcttccatta ccggtcgtcg
    11521 aaattggcgc cggtgtttcc tcatacccgc tcagaatttc actaatcctt ttgaagccct
    11581 gggcgaatgc aatagaacca gccgacacca aggcaatcat tgcaagagac aatcctagtc
    11641 ttcgcatccc tttttcctcc aagattctgt tttgtggggc gcgcataata tcacagcgcg
    11701 ctggaagggg gaacctcata agtccaatat gtcctatagg gcttaagggc gcttttgcgt
    11761 tctggcggcg gttaatttac cttgtctttt ccttatgccg ataagcttgc tcgctctcga
    11821 tctggacggg actctgctcg atgccagggg cattatttct gagcgtaatc gaattgcaat
    11881 cgatcacgcg cgccggcagg gcgtccacgt cgcgctcgtc acgggacggc ggtttcgtga
    11941 ctcgcgtccg gtggcgctgg agttgggact cgacgttccg ttgatctcac ataacggagc
    12001 gttaaccaag catgccgcga cactgcagac cgtttcagtg ttgccgttgc cactcgacgc
    12061 cgcacatgcc tccttgaaga tcgggcgcaa agctgacgcc gatgcgttgc tcagtgacga
    12121 tcacgaaggt ttgggagtgc ttgtttacga tcacatccgc ggcggcaata ctgctgcgca
    12181 caagtacatc tcgtgggcga gtcgcattca cggggacgaa gaaggcacta acgccgtgca
    12241 gcaggttgca tcgctcgaag actacctgga ccacgatccg atccatcttt cattttccgg
    12301 cggctgtgcg gagatggacc gattggaaga gacactcaag tctgagcttg gcgccaccgt
    12361 gaaagtactc ggcacgaaat atctcgagca ggatttcacg ttgctcgaca tcgtcaatgc
    12421 cgctgcgtcg aaaggcgcgg gtgtcgccgc cgctgccgct gagcttggcg tcatgcgtga
    12481 agagatcatg gcgattggcg acaacttcaa cgatctcgag atgctgctct tcgcgggcac
    12541 gggcgtcgtg atggcaaacg cgccgctctt gttgcgcgag attgcgggct tgcacccgac
    12601 tgcttcgaat cgtgacgacg gcgtagcgct cgcgatcgaa caattcatct tgaaccctca
    12661 attaatcaac cagtaaggag aaaacatggc aaatcgaacg gcagtgatcc agacgaacaa
    12721 aggaacgatt aaggtcgagt tgttggagac ggatgcaccc aagactactg agaacttcat
    12781 caccctcgcc gagcgtggtt actacgatgg gatcattttt catcgcgtga tcaagaactt
    12841 catgattcag ggcggcgatc cgacgggtac cggacgtggc ggcgaatcag catggggcgg
    12901 acgtttcaat gacgagatcg acgcgaaatc gcctgtgtac cagcgtggtt ataaagcagg
    12961 gacgatggca atggcgaatg ccggtccaaa tacgaatgga tcgcagtttt tcatcatgca
    13021 cgtcgattat ccgttgccgc cgagttatac gatctttggg aaagtgattg aggggcagga
    13081 tgtcgtgaat gcgatcgcga cttcgcagac gatgaatgac aagcctgtga gtgaagtgaa
    13141 gatggagaag gttacgatcg agtagccacg gattaacacg gattacattc acggattgat
    13201 ctgtgtaatc tgtgaaatct gtggatcata aaggttttgc tccgtgaccg atgcgaggcg
    13261 cagtttttag tgcacggatg gcgtcggtca cgtatttttt ggcagcggcg acggcttgtt
    13321 cgagagttag atctttggcg agattggcgg cgatggcggc ggagagagtg cagccggagc
    13381 cgtggacgtc gccgatttcg atgtagtcag tttgaaactc cgtgaagatg ccgttctcgt
    13441 ctaagagaat gtcgagggcc tggccgccga cgctgcggtg gccgcctttc acgagcacag
    13501 agcgagcacc gatggattga atcgcttctg ccgcgcggcg catgtcggtt tcgcttttga
    13561 ttgagagtcc ggccagcttc tctgcttcgg gaatgtttgg tgtgacgaca cgcgcgagcg
    13621 gaaatagttt cgtcttcagg atctcgaaag cttcatcgtc gatcagcacg tcgcctgacg
    13681 ttgctactac aaccggatcg actaccggcg ccggcaaatt ctcttctgca aacagtcgcg
    13741 ctacttcagc aacgatctcc gcagtcggca acattcccgt cttcgctgcc gcaaccgtta
    13801 gatcctggac gatcggctca acctgcgcgc gcaacgtcgc cgcagactga tgctcggcgc
    13861 cgaacacacc cgtcgtattt tgaaaagtga tcgaggtgag cgccgctgcc ggaaaacaac
    13921 cgagcgccgc gatcgtcttg atgtccgcaa ctaacccagc gccacccgat ggatcgagtc
    13981 cggcgatact gagcacaact ggtctcgtca tttccatctg gtcaggtttt ctatgtcatt
    14041 taacatgcgg atcgcagcga taccgcgcgc acccgcgtga aaacactcat cagcattgtc
    14101 gagcgtgatg ccaccaatcg ccaccaccgg aaactcgccg agctcgcgcg ctacctcagc
    14161 aagcttactc aatccctgcg gctcaccgta agcgcgtttt gactccgttt caaacactgg
    14221 tccaaacacg acgaagtcag caccgccggc acgtgccgca cgcgcctcct cgagcgaatg
    14281 tgtcgagacg ccgatcaaaa actccggacc acaaatattt cgcaccacct caaccggcaa
    14341 agactgcgtc gtcagatgca caccatcagc tcccgcagcc cgcgcgatat ccgatcgatc
    14401 gttgacgagc agacgcgtcc tgctgccgtg cgtgatctcc gtcgcacggg caaccagctc
    14461 gtaaagcacg cgcgcgggta acgacttctc gcgtatctga aacaggggaa cctctgcgtc
    14521 tacagcagcg cgaaggaact ggaggatttc ggagaaatcg tgaggcgacg tcttaccgct
    14581 cgttattgga tagatgatcg gaactggaag cgacatcttc ggtcgagctc ttcctggcgc
    14641 tcgtctcgtt ttgcaaagca gtgacggtgc gtttgaagtt gaagatctcc cagaacgcaa
    14701 tgccgagatt caaaagtccg acaccgctga ccgcgccgcg cacccaaccg gaagcgacca
    14761 ccgcctgcaa accctgaatg cctgtcttgc gggccgcgta cagcagaaag tagttgttac
    14821 cccaatcgct gattccccac atgccgtgcg gccaccacgg cgccaccgtc agaaagattc
    14881 caatctcgag gcacaaaatg atgtaaaaaa taacgcttaa cttcgctgac attccaatct
    14941 ttcttttagc cgcggattca cgcggattaa cgcagatcag cgtttatccg cgaaaatccg
    15001 cggctagtct tgcttttgtc ttaactggct gagtttctcc gcgacctcgc gataagaaac
    15061 atcgactcca taaacctctg tataaaaatc tcgcgcctgc ttcaagtcgc cgagctgctc
    15121 atacgctgac gccagctcgt aacgcagcgc ctggtactcg tcttcgctgt gtcccggcgc
    15181 ctgcaatccc ttcttgaacc agagcactgc cgcttccggc atgcccttct gaataaaaca
    15241 atgcccgagc atgttgcaac actgcaagta ccgcgaggtg ttgtcgccgg gcttcaccag
    15301 actcgccgcg gtttgaaact cctggatggc ctcgtccatg agatccatct ctttatacgc
    15361 agtacccata ttgtaatgcg tctcgtaatc ttcgcgaacc tcgtcgccct cttcagcggc
    15421 gcgaaactct tcaaacaact ctgcgagacc ggcatctatt cccatcgcca ctggtttcgc
    15481 agcgcccaca ttgttgccgt cgtcgacgag tgatgcatac gcgctgtcag cgtcgaaagt
    15541 tatcgactct gcgggaaccg gtgttgcagt cgcgacttcc tcgacaccac caaactcaaa
    15601 caccgttgcc tcttcgacgg gctgatcacg ttgcgtaagc ttctcgcgcc gcgactggat
    15661 ctcaggatgc tgaccaaact gccgttcgag catctcgagc gtgtcgatgg caatgtctga
    15721 gtaaccctgc gcgatgtaga aatcgacact ctcgagttcc tgccgcatca cgttctcgtg
    15781 acgctcttca gcaacaggtg cttcttcagc cgcagcgaca ggcgtctcag gagtgctgac
    15841 gatgctgaag tcgacttcct gaaagtcagt ggctgaagtt gcaaacgatt cgtcttcgtt
    15901 gagatccgca aacgatgcgg tcggatcgct gaaggtcgaa tccggcgccg cgttcgtttc
    15961 aaactcgtcc gatataaccg gcgcgacatc gcccgcttca tcctcgtctt cgacaaccgc
    16021 aaacgtctcg aattgcggca cgtcagtctc gccactctcc ggctcaggta tcgccgcgaa
    16081 ctcttccgga gtttcttcct gcaagccacc gaggagattg agtcgatcga gatagcgttg
    16141 ttcgtccggg gccagtctga ctaactgcgt caacgcgtag cgctcttcat cgacgagctc
    16201 cgatgcttcc gcagactccg caagacgctc cagtgatgat cgcaacgcgt ccatatcgcg
    16261 ctgccaccag tgaatgcgca ccagcattct taaacccgca acgtgatccg gattttgctc
    16321 cagaacctgg ttgacgatct cgagcagctc ccgctcttcg cgtccagcaa gcatccgctc
    16381 gatgatcgtc gagaggatgc gaatcacttc gtctacttct ccggtcttca gataaagacg
    16441 cgtgactgga agaaactgcg gataggtggt cgcgtcctgc gccatcagca gcgacgtcgc
    16501 ccgctcggct ccctttggat cttcagcttc gagataagcg cgcccgagca tcgacaccag
    16561 ctctttatcg tcttcattcg tctcgacaat acggtccagt acttcggcgg cttcgtcagc
    16621 cgtaccgcgc gcgatgtgtg attcgagcat gccgcggatt gcttcacgat cgtcgcgcac
    16681 taattgcagc gctttgttgt aagcgtcgag cgcctgatcg tgtgagccga tctggtgaaa
    16741 acgattcgcc gcctgcacaa acgcagcggc cgactcgcgt ctcatgttct ctttgagata
    16801 accgtcagcg agtttcagac gaatctcggt gttgttcgga tcgagatcgg cgatcttgtg
    16861 gagaatgtcg agtgagcgtt tgttgtcgcc agactttgta taagcctcgg cgactacgag
    16921 atactgcgcc cgtgcatcat ggaccaggcc ctggttggcg tagagatcgg cgagcttgag
    16981 ggcgacgacc ggatcgcgcg ggcgcagccg ctcgatcttc ttgtacatcg cgatggcttt
    17041 gagattgaac tcctgcgcgc tgtagtgctc ggcgatgcgt tcgaagcact ggatggcttc
    17101 ttctttttta ttcgagcgca cataaagatc gcccagcatg ttcaaggtag tgagatcgtc
    17161 agcgtcgtta tcgacgattt gccgatactc cttgatcgca gcgttgaact tcccctgtga
    17221 aaggaatttc tcagcggctt ttaagacctt agctttatcg aaagccatct ggggatctaa
    17281 tttcggttaa tagattgtcg gcggcttcta atcgtagcag cgggcggggc aatgctattt
    17341 cagtcaagcg cgcagtctca actcacttag caagtccggc aaatagccgc gagatacgct
    17401 aacatgcgta aataaagcgt gtcaacgcaa gcatttctgc atgataacga cagcaacgaa
    17461 cggaaaaact ctgcgtggaa aagccgggca gcagatcgct ctcgtgggtc tggggatcta
    17521 cgtcgtgttc gcgccacact cggtggctgc aagcgtgatt ggtgttgcac ttgccgggct
    17581 cggatgggtg atcaggacgc tgcaaacacg cagtttgggg ctgcgttggt ctaaattcga
    17641 cctgatcatc gtcttgtcac tgctctggac tttcgcttcc gctctacttt cggaagagcc
    17701 tcacatcagt gtggcaaagc ttcaggcgtc gtggtgtgtt ttcatctttt acctcgcgcg
    17761 cgcgacgctt acgagacgat cggcattgat tttggtcgcg ttattgattg tttcggcgtc
    17821 ggcgggcgcg ctttacagcg cttttgatct gttgcgcggg cggggagtgg tggtcgagtc
    17881 gatcgcgccg ggcagtccgt tcaaccagat tgggatcaga ccgggcgaca cgatctggca
    17941 gatcaatcga cggcgcgtct attcgaccac tgagatcgat caaatactgg cccaggtgcc
    18001 ggcgaacact gcggtgactg ccggtctgat cacccagggc gaacaggtcg aacgatcatt
    18061 tacattggct cagccggctc tctccggagt tatcggcagc gagtcaaacc atcgctttcg
    18121 tgcttcgggc tggactcgtc actacgagac gttcgccgag ctcatccaga tgatcgcgca
    18181 gctcgcactc ggcttcgccc tcgctcacct tagaaatcac ggtccgaaca agatcttccg
    18241 aatcgcgatt gccgctgctg ctttgctgac ggccgctctc gtgttcaccg caatgagaac
    18301 cgtgatcatc gcgtttgtga tcggggcgag cgtcatggct ttgcgatcgt tgcgcgcgac
    18361 gatgagagtg gcgtttacat ttgcgctttt tgctgtgctt gcttttgcgg cggtagtcgt
    18421 gtggcaaaca cgcgatcgga atgccctctt gctgggcgat ccgagctcgt cactgcgtta
    18481 cgaggttgcg cgtaatggtt tgtcgagaat actgattcac ccgatattcg ggcacggcat
    18541 ggacgcgatg cagttgcact ggaacgagtg gggctttccc ggcaaagaca tgcttcacct
    18601 gcattcgact cctctacaac tggcgttcga tcgtggcttg ccgatgctcg cgctctggct
    18661 gtggatgatg atcttgttct ggctaaccat cgcgcgtgca gaaaaacgcg cagcagatct
    18721 gagtgatact aactcttacg ggattttgct cgggacactc ggcgcactga ccggttttct
    18781 gatgagctcg ctggtgaatt acaactacgg tgacgctgag gtggcgatga tgttttggtt
    18841 cttgatggga atcgggttaa taggtcctat aggacatata ggacctatta ccagtagcga
    18901 ttagattact gcgctccttc accttcaaca cgcagattat tcttcaccga aaacacaccc
    18961 gatacgcggt tcgccgccat gtacgcgagg ttccgatcac cgctgccgga aaccacgcct
    19021 tccaacgtca cgtgaccatt gtcgactacg atgtgcagcg acgggttcgt gccttgcaag
    19081 taccgataaa gcccacccat gcgcgcgatt gatcgatacg tcgcgcgacg aatgttgtcg
    19141 tcaaagcttg ataacggcaa caccctgatg ttgtttacca cacgcgtgac gcccgcaagt
    19201 cttttcactc tgccggctgc gtcagagcgc gtcgaaggac gcaccacttc accgtagagc
    19261 gtgacggtac caccgtctac cttgtatgcg agattatcga atacaccgta atacggcagc
    19321 gtcaccagct ccttccgcac cttcctttcc agttgcggat tgctggcgcg ttgttgagca
    19381 agcgcgacac ccggcgcaac cgccagcaac agcatcgcaa aaattccaat tgcccatttc
    19441 gaaactaagt tattcgtttt catatcctct aaactccctc tgttcctata cgcgttagat
    19501 agacactggc agtgcttggt tggttacgaa attcatcgat ttttgttagg attttcagcc
    19561 aaatgacacg accacaaaca accgaagcag ctgactacta tttccggtac attgaccttg
    19621 tcaccagcga cgacatcgtg cccgcgatcg agaagcagat gggcgagatg ctccagtttc
    19681 tgagcgggat caacgaggaa caaagtcttc attcctacgc accgggaaaa tggaccattc
    19741 gcgaagtgtt aaatcacgtg aacgacggtg agcgagtatt tgcgggccgg gggttctggt
    19801 ttgcgcgcgg gtttaacgac gcgctgccca gcttcgaaca ggacatcgcc gtccaaaacg
    19861 cccatgcgaa taacacttcc tgggcggatc tcgtcgaaga gttcaaaaac gtgcgtctcg
    19921 cgacgatctc attgttcaag agcctgccgg aggaggcgtg ggatcggacg ggagtcgcga
    19981 gtgacaatcc agtttccgtg cgggcaatcg catacatcat cgccggccac gtcgcccacc
    20041 acatcggcgt cctgcgcgaa aagtatcttt agacaagatt ttgcaggatt tcatcatgta
    20101 aatcctgcaa atcctgaaat cctgtcgaaa aacactgttg aatcctgcgc ccgttagggt
    20161 gtactctgga ctcctgcgga gagaactcac tccagcttcc accatccgca ggccccgtcg
    20221 atacaagccc attccaggag actcgcatga aaaggtgccc gaagtgtgaa tttatttacg
    20281 aagacgacca gagtctctgc gacatggacg gcgttctcct cgtcttggac tcccggaagc
    20341 tgcctaatca cggggctctt cagactgtgc ccgcggccaa gacagcgcca aagcgaaacc
    20401 gggccgtgcc ggcgttcgcc actgtaatac tcgctctcgt cctcggtctg gtttattacg
    20461 tttctgtaca ccgcaaagcg acgcaaaaca actacacgcc accaccgccg agcgctgcca
    20521 gcacgtcgaa cccagttcca gccgaagtaa atgtaaatcc aacaccggaa gcacaaccgc
    20581 ccgccgcgga agaaccgaaa cccgaaccgg ccaaacccac agccgctgca gccgctccgg
    20641 cgcccgttaa gaaagtcgcg actaaaccca cgccaacacc agacaagcca aaggtcgagg
    20701 aaaagaaaga cgaatcaaag gtcaactcga tactgaaaaa gaccggtcgg attctcaaga
    20761 agccgttcaa attctgacgt catgtaaact ccccgatgaa acgctgcccc caatgcctct
    20821 tcctctttcc tgactcagac tcgaagtgtg acttcgatca aactccgctc gagcacgtcg
    20881 acgactcagc catcgacggc gccattcgca aacccaaacg ccgcgccctg cctatcgccg
    20941 cggctattgg tctactgctc ggcgtcctga taatcgcgat ctactacggc ctcactcgtc
    21001 aacaacagaa agcttctgct gctacggaaa ctccggtcac aacggtcgaa ccagtcgctg
    21061 cgcctgcagc cacaccatca ccatccgtcg aagtctcgcc ttcaccatca ccttcgatcg
    21121 ctccatctcc gcgatcgtca ccagaaaagc tttcggcggc gcatacacga gcgacgagcg
    21181 atccagtctc gacgagtgga ccaggaatgg gaaaccgtca gggcggcaag cccgtaataa
    21241 tgctgaccag cggcggaaaa atcgaagccg acgaggtctg gcggacgaaa gatggtgtat
    21301 ggtatcgccg caacggtatg gtcacgctac tcaaacgctc tcaggtgaaa acgatcgtca
    21361 cgcgttaagg aggcaagtat gaaagcatcg gcttcccttc tggttctggt tttagtttta
    21421 ggtgtggcta tttcctgcaa gctttcggag cggcttggag gcgacaagaa cgcgggtacc
    21481 gttagtgagc tttggcccga cgtgccgccg tttcagggcg ccaccaaagc tgaggttgag
    21541 attcctttgg gagtgcgatt attgattcgc ggaatgtcgc aagggagagt caacttcatc
    21601 tccttccgca ctgacaaaac cgcgcaggaa gtgaaggact tctattccaa cgatcgcatg
    21661 aaagccgcgg gttggaccgc aaacgacaaa ggctgcaccg gcgacatgga agacaaaaag
    21721 aaccacggcg ccatgtgcat gttctcacgc cgcgacggag gcaaggaaga agccctcgcc
    21781 atcgtcatcg cccaggacga aaaacttcca gacacaaaca tcgtctacgc ccgcatcgat
    21841 acaactaaaa cgaagtaaat cgatctccac ctgcaatcaa ctgtaggggc ggccctccgt
    21901 ggccgcccgt cttcgttttc acactgagag gaaaaactaa tctgctgcgt cattgcttct
    21961 gtagccaagg agcattgatt aaaggagatt agaaatgacc tgcagaagaa ttgttttact
    22021 cgctctcacc atcgcactcg cacttgcctc agtacttgta atgaatccgg gcaaggtgaa
    22081 ttcgatggac gcccagcagc tcccgaataa ccttccccat ccaaaccccg gcggaaaagc
    22141 ggcgaccttc agcacacagg gaaacgtcga tctcaccggt gagttctttc aggcccaggg
    22201 caccaacggg cgtagctgtg cttcatgtca tatcgccgaa gaggcctgga gcattaaccc
    22261 gggcacgtta caagacctct tcgatgaaac cggtggaact cacccggtct tcaacctgct
    22321 cgacgcaaac aatccaaaca tggacgtctc tacaccggca gcgcggctct ctgcttacag
    22381 catgatgttg agtcatggcg tgtttcgccg cggcggtgct ccacgtccga attctgattg
    22441 ggagttgatc gcagtggaag atccacacg
//
LOCUS       DASSFQ010000107        10823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_41998, whole genome shotgun sequence.
ACCESSION   DASSFQ010000107 DASSFQ010000000
VERSION     DASSFQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41998"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..854)
                     /locus_tag="VFH96_03570"
     CDS             complement(57..854)
                     /locus_tag="VFH96_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6973075.1"
                     /translation="MFNNLIESSSHTREYKRRGSFLLFTTGVYAMLLVLGGVASIYAY
                     DARLEEQNLEIVTLLPPVEVIPAAPEPVSRPNQPRETSRNESGIPERATAMLSVNHPE
                     VVPPGISTTPNKNLPLPETGPVRIGTQDRGAGPASGPGTPFTGRVVVPPAQIVTLPDN
                     PPPPPDPPKPARVVSKGPITGLAIFLPKPAYSEIAKRARIQGTVSVQVLVDEYGKVVS
                     AKVLSGHPLLTVDAQRAALQARFSPTKLGDQPVRVSGVITYNFVLSN"
     gene            1016..2284
                     /locus_tag="VFH96_03575"
     CDS             1016..2284
                     /locus_tag="VFH96_03575"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET6973076.1"
                     /translation="MATQKAFQEDLNPYQIAKQQFNRAADYLDLDPSMRHVLENAKRQ
                     LIVSIPVKMDGGDVQVFEGYRVQHNIARGPAKGGIRYHPNVSLDEVKALASWMTWKCA
                     TVGIPYGGGKGGVVCDPKSMSRNELERLTRRYAFEIAPIIGPDRDIPAPDVYTDEQTM
                     AWIMDTISMVRGHTELGVVTGKPISLGGSQGRAEATARGCLYALREACRVKGMSLSGA
                     RVAVHGFGNAGANIARLVAEDGAKVVAACDSKAAVYASTGIDVAEALKHKAATGSLAG
                     LSGAKEIHPEDIISVDCDILLPSALENAITLSNVGKVKAKIIAELANGPTTPGSDRVL
                     EDEGVFLVPDILANAGGVTVSYYEWVQDQYSFFWSERQINDTLEQTMQTAFNSVHQMA
                     ERYNTDMRTGAYILAVGRVTEATSVRGIFP"
     gene            2288..2641
                     /locus_tag="VFH96_03580"
     CDS             2288..2641
                     /locus_tag="VFH96_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET6973077.1"
                     /translation="MADKITVYEKPTCTKCREMDKLLRERGIPYEKINYYIEPLSRKK
                     LTELLHKMNMKPRDLLRKSEAVYKELGLAEDKFSDSELIALMIEHPDLIQRPIVERGE
                     RAVLGRPTENVRELL"
     gene            2649..4010
                     /gene="radA"
                     /locus_tag="VFH96_03585"
     CDS             2649..4010
                     /gene="radA"
                     /locus_tag="VFH96_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET6973078.1"
                     /translation="MKKETVFVCQNCGHATGKWLGKCSECGEWNSLVEEKSQPTRRTG
                     VRNGFKLRDVSAVPFTEIEPQDDVRISSGDTEFDRVLGGGIVPGTLVLIGGDPGIGKS
                     TLLLQVADKLSAAGSLVLYVSGEESERQIKLRGERLAVEAKNVFILPETNLENIINEI
                     ERLKPGAIIVDSIQTVFSSAIESAPGSVSQVREVAHQFLLLAKNRGIPIFLIGHITKE
                     GSIAGPKILEHIVDTVLYFEGERHHNHRIVRAVKNRFGAANEVGVFEMTGAGLMPVAN
                     PSQMFLQERPQNVAGSIVTACMEGTRPLLVEIQALVSGTKYGTGRRMTQGLDQSRVAL
                     LIAMLEKRAGLQLTGDDVFVNIAGGLEVDEPAVDLGVVTAIVSSFKNLTVDPHTAVFG
                     EIGLTGEVRGAMQASVRAREAQALGFKKIVLPASNVPGLERLLGVRVIGVKSVDEALD
                     ELF"
     gene            complement(4019..4492)
                     /locus_tag="VFH96_03590"
     CDS             complement(4019..4492)
                     /locus_tag="VFH96_03590"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET6973079.1"
                     /translation="MIIGIAAVDRKGAIGKGGKLPWHYSADMKFFRETTSGHAVVMGR
                     KTWLTIGKPLKNRLNIVLSRDTDIEPQESLIVFSDIESVLSFNKSLRTDLFVIGGAQI
                     YEAFREHIEKWIITEVPLVVAGADAFMPKGYLEGFDVVESKALEDGLVVKFYERG"
     gene            complement(4504..5352)
                     /gene="thyA"
                     /locus_tag="VFH96_03595"
     CDS             complement(4504..5352)
                     /gene="thyA"
                     /locus_tag="VFH96_03595"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET6973080.1"
                     /translation="MKQYHDLLRSILDNGREHRDRTGVGTISHFGFHTRFNLRKGFPI
                     VTTKRVPFRWVAEELFWFLSGDTSETNLRARGVDIWAEWADEAHTEKFGRPAGDLGPV
                     YGYLWRSFGGAYPEHNGVDQIANLIKQIQMNPDSRRLIVTGWDPRQAENVDLPPCHTL
                     FQFKVENGKILHCQLYQRSADAFLGVPFNISSYALLTHLIAHVTDLDVGDFVYTLGDY
                     HIYRNHLEQVQQLLSREPLPLPQLKINDPENKLRGLEGLLAVKYEHLELAGYQSHGKI
                     AAPVAV"
     gene            5377..6387
                     /locus_tag="VFH96_03600"
     CDS             5377..6387
                     /locus_tag="VFH96_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973081.1"
                     /translation="MKLYRICFLGFGNVGRALARLFVSKSAELRDAYGIEWEIAGVAT
                     RRMGWRASENTIDVTSLLNGSDDTASVPGIDEWLARAKPDVVFETTSLNPETGQPAIE
                     YLRKSLQAGAHTITANKAPLVYAYDELNALAVAQGRRFMFEATVLDSAPVFSLFRETL
                     PAVRVRGFSGAFNSTTNVIIETMEAGRSFAEGVKTAQELGVAETDPSHDVDGWDSIMK
                     VCAMARVILRVPLLPSQVRREGIRGLDPATLRAARAEGRPYKLVSRVTVNADGSVTAS
                     VRPEQVAASDPLGGVRGTSLAIHFELDVIPGLTITSHRPNLQSTAYGLLADFLNADRS
                     RS"
     gene            complement(6405..7640)
                     /locus_tag="VFH96_03605"
     CDS             complement(6405..7640)
                     /locus_tag="VFH96_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HET6973082.1"
                     /translation="MSTTSLQPQVASVTEIRSHFPALERVHNGYPVAYFDGPGGTQVP
                     RYVVEKMADYLYHHNANTHWAYPTSAETDAALEHAREVYAEFLNASPTEIAFGANMTT
                     LTMHLSRALSLSYQPGDEIVVTELEHHANIDPWRRLTVERGVTIRTVRIDTATGQIDY
                     DDLQRAIGPKTKLVAIGAASNALGTINDFKSVIAMAHDAGALVFVDAVHYAPHALIDV
                     KELDCDFMGMSAYKFYGPHIGVLFAKREYYEQIDFPRLVPAPDYAPENAETGTQNHEG
                     MVGAAAAVEYLASLGGGINLRENLRHVFHETHARNLALFTRLWNALSAMPRVKLYGPP
                     PDSARTPTIAFTIDGYTSTDAAERLSEQGLFLSHGDFYAYTIVERLGLHPEGFIRAGC
                     GIYTTEDEIDRLIDAVSKL"
     gene            7772..8086
                     /locus_tag="VFH96_03610"
     CDS             7772..8086
                     /locus_tag="VFH96_03610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6973083.1"
                     /translation="MSDRRSEKRWDVCLDAIWDGKSGNYVARVTDLSEGGCYVDSLGE
                     AQIGEVLTFKLQLPDGEWLALTGEVAHQSPPLGFGLRFVTLSDEQREKLHSILDHVQP
                     IS"
     gene            complement(8155..9555)
                     /gene="cysS"
                     /locus_tag="VFH96_03615"
     CDS             complement(8155..9555)
                     /gene="cysS"
                     /locus_tag="VFH96_03615"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET6973084.1"
                     /translation="MLKFRNTLSGRVEEFRPLREGEVGFYYCGPTVWNYGHIGNFRSA
                     IAADIVVRYLKFKGYKVTHVMNITDVEDRIIAKSQEAGLTIDEYTAKYIDALWEDFDA
                     LGCERPTVVPRATRHIPEMVTLIEKLLDSDHAYRSDDSIYYRIASFPEYGKLSKINFA
                     GNIAGASERIDTDRYEKEDARDFALWKEPSSETEPAWETTIGRGRPGWHIECSAMSMK
                     YLGETFDIHAGGIDLVFPHHENEIAQSEGATGKEFVRYWIHFEHLKVDGETMSKTKGN
                     YYTFRDVTKKGFSAAAVRYFLLSVPHNKQLNFTFDALAGAERTVASLRDFRARLSEAK
                     TEPGKDEKLSENAARALEEFEAGMDDDFNTSIALAAIHNLSREVNTALARKKVKAENQ
                     QELLALIDRFDSVLNIFGKQEREMLDSEVQALIDERQEARRRRDFARGDEIRDELASR
                     GIILEDTKDGVRWKRK"
     gene            complement(9612..10619)
                     /gene="thiL"
                     /locus_tag="VFH96_03620"
     CDS             complement(9612..10619)
                     /gene="thiL"
                     /locus_tag="VFH96_03620"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET6973085.1"
                     /translation="MRSEFDFINSLRQRVGSSSQSIVTGIGDDAAVFRNSPGKETVIS
                     TDLLVEDIDFRRSTTPPYLLGHKALAVSLSDLAAMGSRPLWSLISIGVPENVWESDFI
                     DRFYDGWLDLANRYGVQLIGGDTSRTNERTVIDSIAIGECSAGMAVKRSSAKAGDQIF
                     VTGSLGAAAAGLRLIERGAHLAEQNLGDEDSQKLDHVLLRQLRPEPRVGWGIVLGEER
                     IASSMIDLSDGLSSDLNHLCAASDVGALIDASLLPIDERVVELCGRRALDPLQLALHG
                     GEDFELLFTVKPENVARLPRRVDGVEIKRIGEVMSANYGVKISEGSRIWDLKPGGWKH
                     F"
     gene            complement(10622..>10823)
                     /locus_tag="VFH96_03625"
     CDS             complement(10622..>10823)
                     /locus_tag="VFH96_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the transamination of the aromatic amino
                     acid forming a ketoacid; first step in aromatic amino acid
                     degradation in lactococci; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6973086.1"
                     /translation="AFYAFPDVSRINKDSREAADVLLNRAQVATVPGIVFGAKGESHL
                     RFSFSTSIETIEAGLDSLRRNL"
BASE COUNT         2345 a         2926 c         3125 g         2427 t
ORIGIN      
        1 agggcgggag aggtacggaa cgccatgtcc cgtacctctt ttgctgggca ggagtctcaa
       61 ttactaagta cgaagttgta ggttatgact ccggaaactc tgactggttg atcgcctagc
      121 tttgtcggcg aaaagcgtgc ctgtagtgcg gctctttgtg cgtcgactgt caggagcgga
      181 tgtccgctca acaccttcgc cgaaactacc ttcccgtatt cgtcaaccaa aacctggaca
      241 ctgactgtgc cctgtatgcg tgcccgtttt gctatctccg aataagctgg cttaggtaaa
      301 aaaatcgcca gtcctgtgat cggtccttta ctgaccaccc tcgctggttt cggtggatca
      361 ggaggtgggg gcgggttatc cggcagtgtg acgatttgag caggtggaac tactactctt
      421 ccagtgaatg gagttcctgg accactagcc ggtccggcac cgcggtcttg agtaccgata
      481 cgaactggac cagtttcagg caacggcaag tttttgttgg gtgtggttga gataccgggg
      541 ggcacaacct ccgggtgatt cacgctaagc attgccgtcg cgcgctcagg aatacccgat
      601 tcattgcggc tggtttctct tggttgattc ggacgtgata ctggttcagg tgctgctgga
      661 atgacctcca ccggtggcag cagcgtaacg atttctaaat tctgttcttc aagacgcgca
      721 tcgtaagcgt aaatacttgc caccccgcct agcaccagca acatagcgta aacgccagtg
      781 gtgaagagca gaaacgaccc gcggcgtttg tattcccgcg tgtgcgaact ggattcaatc
      841 agattattaa acatcgagtt cctccgctat ttgccggtgc cattcgggtc gcaggagcta
      901 ctcggctttt tagacaccat attgccccgc gggttcccac ttcgcttgaa aacctgttgg
      961 cgcacgcata cgattactgc cgctccgagc cccattcttg ccaggaggag attgcatggc
     1021 gacccaaaag gcctttcagg aagacctcaa cccgtatcaa attgcgaaac aacaattcaa
     1081 tcgtgcggct gattatctcg acctcgatcc ttctatgcga cacgtgctgg aaaatgccaa
     1141 gcgacaactg atcgtctcga ttccagtgaa gatggacggc ggtgacgtgc aggtgtttga
     1201 aggttatcgc gtgcaacaca acatcgcgcg tggaccagca aaaggcggca ttcgctacca
     1261 tccaaacgtc tcgctcgatg aagtgaaagc gcttgcttca tggatgacct ggaaatgtgc
     1321 gactgtcggt attccgtatg gcggtggtaa aggtggagtg gtttgcgatc cgaagagcat
     1381 gtcgcgcaac gagctggagc gactgacgcg acgttacgct ttcgagatcg cgccgatcat
     1441 tggtccggat cgtgacattc ccgcgcccga tgtttatacc gacgagcaaa caatggcgtg
     1501 gatcatggac acgatctcca tggtgcgtgg tcacacggag ctcggcgtcg ttaccggcaa
     1561 accgatctcg ctcggtggat cgcagggacg tgctgaagcg actgcgcgcg gttgtctcta
     1621 tgcgttgcgt gaggcttgtc gcgtgaaagg aatgagcttg agcggcgcgc gtgtggcagt
     1681 gcacggcttc ggaaacgcgg gcgcgaatat cgctcgactc gtcgccgaag atggcgcgaa
     1741 ggtcgtcgca gcgtgcgatt cgaaagccgc agtttatgcg agcacgggta tcgacgtcgc
     1801 tgaggcactg aaacacaaag ccgcaaccgg ttcgctggcg ggactgagtg gcgcgaagga
     1861 gatccatcct gaggacatca tcagtgtcga ctgcgacatc cttttgccgt cggcgctcga
     1921 aaacgcgatc acccttagca acgtgggcaa agtgaaggcg aagatcattg ccgaactggc
     1981 gaatggtcca acgacgccgg gatccgatcg agtgctggaa gatgagggag ttttcctcgt
     2041 gcccgatatt ctcgcgaatg cgggtggcgt gacggtgagt tactatgagt gggtgcagga
     2101 tcagtattcg ttcttctggt cggagcgcca gatcaatgac acgctcgaac agacgatgca
     2161 gacggcgttc aattcagtgc accagatggc tgagcgttac aacaccgaca tgcggaccgg
     2221 tgcatatatt ttggcagtgg gaagagtaac tgaagctacg agcgtgaggg ggatatttcc
     2281 gtaagtcatg gcagacaaga tcacagttta tgagaaaccg acttgtacga agtgtcgcga
     2341 gatggacaag ctgctccgcg agcgcggcat tccgtacgag aagatcaatt actacatcga
     2401 gccgttgagt cgaaagaaac tcactgagct gttgcataag atgaacatga agccgcgcga
     2461 tcttttgcga aagagcgagg cggtttacaa agagctcgga ctcgccgagg acaagtttag
     2521 tgatagcgag ttgatcgcgt tgatgatcga gcatccggat ctgattcagc ggccgatcgt
     2581 cgagcgcggc gagcgcgcgg tgctgggtcg accaaccgag aatgtgagag agttgttata
     2641 gttactgcgt gaaaaaagaa acggtctttg tttgtcaaaa ctgcgggcac gcaaccggca
     2701 agtggctcgg caagtgttcg gaatgcggtg agtggaattc actcgtcgaa gaaaaatcgc
     2761 aaccaacgcg tcgcaccggc gttcgcaatg gcttcaaact ccgcgatgtt tccgctgttc
     2821 ctttcacgga gatcgaacca caggacgacg tccgcatttc ttccggcgac actgaattcg
     2881 atcgcgtact cggtggtggc atcgtgcccg gcacactcgt cctgatcgga ggcgatccag
     2941 gtattggcaa gagcacgctg ttgcttcaag tggcggacaa actgagcgcc gccggatcgc
     3001 tcgtgcttta cgtctcgggt gaagagtcgg aacggcagat caagctgcgc ggtgagcggc
     3061 tcgcagtcga agcaaagaac gtcttcatcc ttcccgagac taatctcgaa aacatcatca
     3121 acgagatcga gcgtctgaag cccggcgcga tcatcgtcga ctcaattcag actgtctttt
     3181 cgagtgcgat cgagtcggcg cccggatcgg tttcgcaggt gagggaagtg gcgcaccagt
     3241 ttctcctgct cgcgaagaat cgtggtattc cgatttttct gatcggacac atcacaaagg
     3301 aaggctcgat agctggtccg aagattctcg aacacatcgt cgatacagtg ctctacttcg
     3361 aaggtgagcg tcatcacaat catcgcatcg tgcgtgccgt aaagaaccgt ttcggcgctg
     3421 cgaatgaagt cggagtgttt gagatgacgg gcgccgggtt gatgccggtg gccaatccgt
     3481 cgcagatgtt tttacaggaa cgaccgcaga acgtggcggg atcgatcgtc acggcatgta
     3541 tggaaggcac gcgacctctg ctcgttgaga ttcaggcatt ggtttcagga acaaagtacg
     3601 gaaccgggcg ccgcatgacg cagggcctcg atcagagtcg ggtcgcgctt ttgatcgcga
     3661 tgctcgagaa acgtgccggt ttgcaactca cgggcgacga tgtgttcgtt aacatcgcag
     3721 gcggactaga ggttgacgaa cctgctgtag atctcggcgt cgtaacagcg atcgtatcca
     3781 gtttcaagaa cctgactgtc gatccgcaca ccgcagtctt tggcgagatt ggcttaactg
     3841 gcgaagtacg cggcgccatg caggcctccg tgcgcgcccg cgaagcccag gctctcggat
     3901 ttaaaaagat cgtcctcccc gcctcaaacg tccccggcct cgagcgctta ttaggtgtgc
     3961 gcgtcatcgg tgtaaaatcc gtcgatgaag ccttggacga actattctaa atccgcgttc
     4021 atccgcgttc atagaacttc accaccagcc cgtcttcgag ggcttttgac tccaccacat
     4081 caaacccctc caaataaccc ttcggcatga acgcatccgc acccgcaact acgagcggca
     4141 cctcagtgat gatccacttc tcgatatgct cccggaacgc ttcatagatc tgcgcaccgc
     4201 cgatcacgaa caagtcagtc ctcagcgatt tattaaacga caacaccgac tcaatatcgc
     4261 tgaacacgat caacgactcc tgcggctcaa tatctgtatc gcgactgagc acgatgttga
     4321 gcctgttctt cagcggcttc ccgattgtga gccacgtctt ccgtcccatc actaccgcat
     4381 gtccgctcgt tgtctcacga aaaaacttca tatccgccga gtaatgccag ggcagcttcc
     4441 caccttttcc tatcgcgcct tttcgatcga ctgctgcaat cccgatgatc atgatgtttg
     4501 tctctatacc gcgacagggg cggcgatttt gccgtgtgat tgatagccgg ctagttccag
     4561 gtgttcgtat ttcactgcta acaatccttc gagaccgcgc agtttgtttt ccgggtcgtt
     4621 gatctttagt tgcggtagcg gcagcggttc gcgcgacagg agttgttgca cctgttcgag
     4681 atgatttctg tagatgtggt aatcgccgag cgtgtagacg aagtcaccga cgtcgagatc
     4741 tgttacgtgt gcgattaagt gcgtcagcag cgcgtaagag ctgatattga agggcacgcc
     4801 gagaaacgcg tcagcggaac gttggtagag ttggcagtgc aggattttgc cattttcgac
     4861 tttgaactga aacagcgtgt ggcacggcgg aagatcgacg ttctcggctt gtcgtggatc
     4921 ccaaccggta acgatcagtc tgcgtgagtc aggattcatt tggatctgtt ttatgagatt
     4981 cgcgatctga tcgacaccgt tgtgttcggg ataggctcca ccaaacgatc gccacagata
     5041 gccataaact ggtccaagat ctcctgccgg tcgtccgaac ttttccgtgt gcgcctcatc
     5101 cgcccactca gcccagatat caacaccgcg cgcgcgcagg tttgtctcgc tcgtatctcc
     5161 cgacagaaac cagaacaact cctcagccac ccagcgaaac ggcacacgct tcgtcgtgac
     5221 gatcggaaat cccttgcgca gattgaagcg tgtgtgaaat ccgaaatgcg aaatcgtacc
     5281 gacgccggtg cgatcgcgat gctcgcggcc gttatcaagt atagatctta gaagatcgtg
     5341 gtattgtttc atgacgggag tcctatccaa atatacatga aactatatcg aatctgcttc
     5401 cttggttttg gaaacgttgg gcgtgccctc gcgcgactgt ttgtttcgaa gtcggcggag
     5461 ttgcgtgacg catacggtat cgagtgggag atcgcggggg tggcgacgcg gcggatgggt
     5521 tggcgcgcga gtgagaatac gatcgatgtg acgagtttgc tcaatggcag tgatgacacg
     5581 gcgagcgtgc caggcatcga tgaatggctc gcgcgtgcaa agcctgatgt ggtgtttgaa
     5641 acaacgtcgc tgaatccgga aacgggtcaa cccgcgatcg agtatctgcg aaagtcgctg
     5701 caagccggcg cgcataccat caccgcaaac aaggcgccgc tcgtttatgc gtacgacgag
     5761 ttgaacgcgc tcgcggtggc tcaaggcagg cgattcatgt ttgaagcaac ggtgctcgat
     5821 agtgcgcccg tgttctcact ctttcgcgag acattgccgg ccgtgcgagt acgtggtttc
     5881 agcggcgcgt tcaattcaac tacaaacgtg attatcgaga cgatggaagc gggccggagc
     5941 ttcgctgaag gcgtgaagac cgcgcaggag ctcggtgtcg ctgaaactga tcccagtcac
     6001 gatgtcgatg gctgggactc gatcatgaaa gtttgtgcga tggcgcgcgt tatcttgcga
     6061 gtgccgttgc tgccgtcgca ggtgagacgc gagggaatac gtggactcga tccggcgacg
     6121 ttgcgagcgg cacgagccga aggccggcct tacaagttag tctcacgcgt gacggtcaac
     6181 gcagatggat cagtgacagc ttcagtcaga cccgagcagg ttgctgcgag tgatccgctc
     6241 ggtggtgtgc gcggtacatc gctcgcgatt cactttgagc ttgatgtaat tcccgggttg
     6301 acgatcacgt cgcaccgccc gaatctacaa agcactgctt atgggctgct cgccgatttt
     6361 ttgaacgcgg atcgaagccg atcttaaaag cagattttcg cggatcagag tttggataca
     6421 gcgtcaatca aacgatcgat ctcgtcttct gtggtgtaga tgccgcagcc tgcgcggatg
     6481 aatccttccg ggtgtaagcc caggcgttcg acgatcgtgt aagcataaaa gtcgccgtgc
     6541 gagaggaaga ggccttgctc cgataaacgc tcggcagcgt cagttgaagt gtagccgtcg
     6601 atcgtgaaag ctattgtcgg tgtgcgagca gaatcaggtg gcgggccata gagcttcacg
     6661 cgcggcatag cgctcaacgc gttccacagg cgagtgaata gcgcaaggtt gcgcgcgtgc
     6721 gtttcgtgaa agacgtggcg aagattctcg cgcaaattaa tgccgccgcc gagtgacgcc
     6781 agatactcga ctgccgcggc tgcgcccacc attccctcat gattctgcgt gcctgtctct
     6841 gcgttctcgg gtgcgtaatc gggcgcgggc acaagtcgcg gaaagtcgat ctgttcgtag
     6901 tactctcgct tcgcgaaaag cacgccgata tgtggtccat aaaacttgta tgccgacatt
     6961 cccatgaaat cacagtcgag ctccttcacg tcgatcagcg cgtgcggtgc gtagtgcacg
     7021 gcatcaacaa acacgagtgc acccgcatcg tgggccatcg cgatcacgct cttgaaatca
     7081 ttaatcgttc cgagcgcgtt tgaagcggcg ccgatcgcca ccagctttgt ttttggtcca
     7141 atggcgcgtt ggagatcatc gtagtcgatc tggccggttg ctgtgtctat tctcacggtg
     7201 cgaatggtta cgccacgctc gactgtcaga cgacgccacg gatcgatgtt cgcatgatgc
     7261 tcgagctcgg tgacgacgat ctcatcgccg ggctggtagc tcaggctgag tgcgcgcgaa
     7321 aggtgcatcg tgagcgtcgt catgtttgcg ccgaaagcga tctcggtggg cgacgcgttc
     7381 aggaattccg cgtagacctc gcgagcgtgt tcgagcgcgg cgtctgtctc ggcgctcgtg
     7441 ggataagccc agtgggtgtt cgcgttgtga tggtaaaggt agtcggccat cttctcgacg
     7501 acgtagcgcg gtacctgcgt accgccgggg ccgtcgaaat atgcgaccgg atagccgttg
     7561 tggacgcgtt cgagagcggg gaaatggctt ctgatttccg tgactgatgc gacttgtggc
     7621 tgcagggaag tggttgacat gaggaggcat gataacaaaa gtcgttccca tttcctgccg
     7681 gtgcgggtta gaatgaattc ggcaagccga cgcgctcacc ctgccgccgg ctggaacgcc
     7741 gatcaccaaa acagcagtcg ttgactcacc tatgagcgac cgcagaagcg aaaaaagatg
     7801 ggacgtttgt ctcgacgcga tttgggacgg taagagcgga aattacgtgg cccgcgtcac
     7861 agatttgtcc gaaggtggct gttacgtcga cagtctggga gaagcccaga ttggtgaagt
     7921 gctcacgttc aagctgcaac taccggacgg cgagtggctc gcgctcacgg gcgaagtcgc
     7981 gcaccagtca ccgccactcg ggtttggctt gcgatttgta actctttcag acgaacagcg
     8041 cgagaagctt cactcaatcc tcgatcacgt tcaaccgatc agttagccac ggataatacg
     8101 gattttaaaa ccagatccgc gctgatcaga aataaatccg tgttatccgt ggccctattt
     8161 gcgtttccag cggacgccgt ctttggtgtc ttcgaggatg atgccgcggc tcgcgagctc
     8221 gtcacggatt tcgtcgcccc gcgcgaagtc gcgccgccgc cgcgcttcct ggcgctcatc
     8281 gatcaacgcc tgaacttcac tgtcgagcat ctcgcgttcc tgcttgccga agatattcaa
     8341 gactgaatca aagcgatcga tgagggcgag cagctcctgc tggttctcgg ccttgacctt
     8401 tttgcgcgcg agcgctgtgt tcacttcgcg tgagagattg tgaatagccg cgagcgcgat
     8461 ggacgtgttg aaatcgtcgt ccatgcccgc ttcaaattcc tcgagcgcac gtgcggcatt
     8521 ttcagataat ttctcgtctt tgcctggctc agtcttcgcc tcactcaagc gcgcgcgaaa
     8581 gtcgcgcaga ctcgcaaccg tccgttccgc gccggcgagc gcgtcgaaag tgaagttgag
     8641 ctgtttgttg tgcggcacac tcaacagaaa gtatcgcacc gctgcggctg agaaaccttt
     8701 cttcgtaaca tcacgaaacg tgtagtagtt gcccttcgtc ttcgacatcg tttcgccgtc
     8761 gactttcagg tgctcgaagt gaatccagta acgaacaaac tcttttcccg tcgcgccttc
     8821 actctgcgcg atttcgtttt cgtgatgtgg gaagacgaga tcgatgccgc ccgcgtgaat
     8881 atcaaacgtc tcaccgagat acttcatcga catcgccgag cactcgatat gccagcccgg
     8941 acgcccgcga ccgatggttg tctcccacgc gggttcggtt tcgctcgacg gttctttcca
     9001 gagcgcaaag tcgcgcgcgt cttctttctc gtagcgatcg gtgtcgatgc gttcactcgc
     9061 acccgcgatg ttgcctgcga agttgatctt ggaaagcttg ccatactcgg gaaacgaagc
     9121 gatgcggtag tagatcgaat cgtctgaacg atatgcgtgg tccgaatcga gtagtttctc
     9181 gatcagcgtg accatctcgg gaatgtgacg cgtggcgcgc ggcacgacgg tgggccgctc
     9241 gcagccgagc gcgtcgaaat cttcccagag cgcgtcgata tacttcgcgg tgtactcgtc
     9301 gatcgtcagg ccggcctctt gcgacttggc gatgatgcgg tcttcgacgt cagtgatgtt
     9361 catgacgtgc gtgactttgt agcctttgaa cttcaggtaa cgcaccacga tgtcagcggc
     9421 gatagcgctg cggaagtttc cgatgtgacc gtaattccag acggtaggac cgcagtaata
     9481 gaagccgacc tcgccctcgc gcagcgggcg aaactcttcg acgcggccag ataaagtatt
     9541 ccgaaacttg agcatgggac gttgaggata aggtagatta tcggggattt acaaatccgt
     9601 gtaatccgtg gctaaaaatg cttccagccg ccgggcttga gatcccagat gcgtgagcct
     9661 tctgaaattt tgaccccgta gttcgcgctc atcacctcgc cgatgcgttt gatctcgact
     9721 ccgtctactc gtcgtggcag gcgcgcaacg ttctccggct taaccgtgaa cagcaactcg
     9781 aagtcttcac caccatgcaa cgcgagctgg agcggatcga gcgcacgacg cccacacagc
     9841 tccacgacac gctcgtcgat cggcaacaac gacgcatcga tcaacgcccc gacatcactg
     9901 gccgcgcaca gatgattaag atcggacgac aagccatcgc tgagatcgat cattgagctg
     9961 gcgattcgtt cctcgcccag cacgataccc cagccgacac gtggctccgg tcgaagctgc
    10021 cgcagcaaaa cgtgatcgag tttttgcgaa tcttcgtcgc caagattctg ctcggcgaga
    10081 tgcgcgccac gttcgatcag tcgcaaaccc gccgctgcgg cgccgagcga accagtaaca
    10141 aagatctgat ctcccgcctt cgcactgctt cgcttcaccg ccattcctgc ggaacattcg
    10201 ccgatcgcga tcgaatcgat caccgtgcgc tcattcgtgc gtgacgtgtc gccaccgatc
    10261 aactgcacgc cataacgatt cgcgaggtcc agccaaccgt cgtagaaccg atcgatgaag
    10321 tctgattccc aaacattttc cggcacgccg atggaaatca gagaccagag cggtctcgat
    10381 cccatcgccg caagatcgga aagtgaaacg gcgagtgctt tatggcccaa caggtaaggc
    10441 ggtgttgtcg agcgacgaaa gtcgatgtct tcaaccagca gatcggtgct gatgacggtt
    10501 tcttttccag ggctgttgcg aaagacagca gcatcgtcac cgataccggt cacgatcgat
    10561 tgcgatgaag acccgactcg ctggcggagt gaattgatga agtcgaattc gcttctcatc
    10621 gttagagatt acgacgaagc gaatcgagcc ctgcttcgat ggtttcgatc gaagtggaaa
    10681 aagagaagcg caggtggctc tcgcctttcg cgccgaagac gatacccgga accgtcgcga
    10741 cctgtgcgcg gttgagtaac acatccgcag cttcgcggct gtctttgttg atgcgactca
    10801 catccgggaa agcatagaac gcg
//
LOCUS       DASSFQ010000108        14615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1683476, whole genome shotgun sequence.
ACCESSION   DASSFQ010000108 DASSFQ010000000
VERSION     DASSFQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14615
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683476"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFH96_03630"
     CDS             complement(<1..517)
                     /locus_tag="VFH96_03630"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HET6973087.1"
                     /translation="MSRNTRAPEIIDVGPRTRRRWKIWLVVAAILLLFSLSRIVSIYI
                     SALWFGSLGYSSVYWYVFKAKLALFFGSAILTALLLSATFLLFQRLFGAYAFEQRTIV
                     LNNQPFQFSPARFIRPLGWVVSGVFGLIYGFMLKEKWRQFALYFNQPSTATHDPIFGK
                     SLGFYLFSLPFY"
     gene            574..2106
                     /locus_tag="VFH96_03635"
     CDS             574..2106
                     /locus_tag="VFH96_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6973088.1"
                     /translation="MLTPETVLQGRYRIVRQLGQGGMGAVYEAIDQRLDTTVALKETL
                     FSEERLRKQFEREARLLARLHHPALPRVSDHFSEADGQFLVMQFIPGDDLSEMMNRKR
                     GPFPANQVLTWADQLLDALDYLHTQDPQIVHRDIKPQNLKLTARGQIILLDFGLAKGQ
                     AGEISRVTTAASIFGYTPNYAPLEQIQGLGTDSRSDLYSLAATLYHLMTGVKPPDALT
                     RAAALVNGQPDPLLKASEANSEISPDIDEVLAKAMAQNREQRYATAADMRRALHEAEQ
                     ASTVVNRGEAQTVLLPAPPPKTVPVQTQTAATPQTVGPTGETTVVRPKGSGPGRLVPV
                     AILAAVLVLIGGALGFFALKKHNQQDAPVATESPAPLGGPAADTPSPSPEAKTSAEQA
                     AATPKKTEKTARNEEPKRAPAPEKRAEPRADVDAKGHDEEDEPPDTPVPPDFQRQNRP
                     RRPMVRVMPGGVKIQTLPDGTQIITTGDGGRFMIGPDGKRTVLNPARPRRRPAPAPTP
                     EP"
     gene            2132..3754
                     /locus_tag="VFH96_03640"
     CDS             2132..3754
                     /locus_tag="VFH96_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET6973089.1"
                     /translation="MYKRKTFRVPTFALLALFACLAMLTSVDVAVRAKNERVIPSNIR
                     ITPPAPAFVDKDRVAELKQRRERVAQSIGPQSFLILFSTEPRVYTNDVDYPYRQENNL
                     YYLTNLKQKGATLVLLPGNTKIREILFMPRRNPGAETWTGHMYSAEEAKQLSGVSEIW
                     QASEFEPFIAALRNRQPYRPKPEAIFMSDLPLDMPLNNDHGYTQLFDAAAKGEAGLFM
                     LVPPGQGENREYKQEQRFASNWSSTASGYSVKNAWPIFTEMRLVKSPMELRLLQHAID
                     ISTEAHQRAWAAAGEAKWEYEVDAVMAYTFKLRNADNWGYPDIVGCGPNATTLHYIES
                     QGQVKQGDLLLMDVGAEYDHYTADITRTFPVNGKFTPAQAAVYQIVYDAQEAVAKASK
                     PGATLSDVNRAGTEVVKDGLLKLGLITDRNSNQHRIWFMHGTSHWLGMNVHDVGGGAR
                     FVPGVTFTNEPGIYVRPDALDQMPYGWKQEDWEKFKTAVRPAFEKYKGMGVRIEDDMV
                     ITADGVRWMSEALPRKISDIEDFIAKARRQSE"
     gene            complement(3798..4346)
                     /locus_tag="VFH96_03645"
     CDS             complement(3798..4346)
                     /locus_tag="VFH96_03645"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HET6973090.1"
                     /translation="MKLEGTHELDAKRERVYQCLITPEVLQRCIPGCEKLEQTAENTF
                     AATIRAGVGSIKGVFNGTARLEDLREPEHLRIVVDGKGQPGFLKGSGDLDLEQTENGT
                     KVSYSGDVQVGGTIASVGQRMIQGTTKMMATQFFTSLAAEAKTAVGEPPPQHGFFRTA
                     LRWASGFLKRLFRPQKGTKSTK"
     gene            complement(4343..5209)
                     /locus_tag="VFH96_03650"
     CDS             complement(4343..5209)
                     /locus_tag="VFH96_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET6973091.1"
                     /translation="MIPVQFDYKTPATLAEAVALLASDLDGAKVLAGGHSLIPAMKLR
                     LVQPQLLVDIARIKSLAYIREEGDQILIGATTTHYQLESSDLLQRICPLLPECAKSIG
                     DVQVRNKGTIGGSIAHSDPAGDWPAAVLALDADLVLVGPNGERTVKAENFFVDLLTTD
                     LQPGEILREVRIRKPSGRFGHAYQKVPHPASGFAVVGIAVNLELNDDGSCKSARIGVT
                     GVGVKAYRAKAVEAALAGTNLDDNAVAAAVAHVCDGIDPNSDLYASGDYRCHLAQVHT
                     RRGIQTAKARVQ"
     gene            complement(5219..7624)
                     /locus_tag="VFH96_03655"
     CDS             complement(5219..7624)
                     /locus_tag="VFH96_03655"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6973092.1"
                     /translation="MTENYVGKRVKRTEDPRLIRGLAHYVDDIALPGTLHVAFVRSMY
                     AHAKINSIDTSAALAAPGVVAVYTGHDVAEKIGPVPCASALPELKVPDHRVLAKDKVY
                     FVGHPIAAVVAKDRYAARDAVDLVMVDYEDLPVVTDVEQAAAGGPVIHEAFGSNIAYK
                     LTSGEGDIDAAFAAADRTVKQRILHRRLAPIAMEPRGVLARYFPGEEELTLWSSTQIP
                     HLMRTQVALMIGMPENKLRVITPEVGGGFGSKLNVYAEEALLSWISIQLGKPVKWIET
                     RRENMAATIHGRGQVGDIEVAFKNDGTLLGLRYSVFADLGAYHQLLTPAIPTLTGLML
                     SGAYKIPAIQMNIVGCFTNKMATDAYRGAGRPEATYVVERALDLVAVDLGVDVAELRR
                     KNFPAADEFPFHTATGLDYDSGDYETALNKAQEIAGYADLRDKQRKAREEGRLIGIGL
                     STYVEICALGPSQAMPAGGWESATVRIEPTGKVTVMTGASPHGQGQETSFAQIAADEL
                     GVNLNDVTVIHGDTGIVQYGIGTFGSRATAVGGTAVFVAIQKLKEKAHKIATHMLKAD
                     ASHVAFEGGKYSLEKAAAATAATGTSDPVVPVGEAPAGALPEPQTEGKTSLTIQDIAL
                     AAHLAKELPPDTEPGLSATYFFEPKNFTFPFGTHIAVVEVDRDTGDIEFLRYVAVDDC
                     GRVINPMLVDGQIHGGIVQSIGQAMFEEVVYDEQGQLITGTLMDYALPRASHVPEFEL
                     DRTETPSPVNPLGVKGVGEAGTIGATPAIVNAIVDALAPFGVKHLDMPVKPENIWRII
                     NGH"
     gene            complement(7670..8143)
                     /locus_tag="VFH96_03660"
     CDS             complement(7670..8143)
                     /locus_tag="VFH96_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6973093.1"
                     /translation="MKKAINLNVNGVSHTDEVEPRMLLVHYLRDVLELTGTHVGCETS
                     LCGACTVMLDGQAVKSCTVFAVQADGSSVTTIEGLAKNGELHPVQEGFWERHGLQCGY
                     CTPGMIMTAAQLLDRNADPSREEICHGLEGNLCRCTGYQHIIEAVQYAAKKAGHR"
     gene            complement(8173..8496)
                     /locus_tag="VFH96_03665"
     CDS             complement(8173..8496)
                     /locus_tag="VFH96_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973094.1"
                     /translation="MAELTYEQLRHKTLAELREMAKGNEHEAVQGYTQMNKEHLAVAL
                     AKALGIQHEHHSVVGIDKSSIKARIRELKTQRAAAIEAHDRAQLKTVRRTIHRLKRRI
                     HKATV"
     gene            complement(8506..9009)
                     /locus_tag="VFH96_03670"
     CDS             complement(8506..9009)
                     /locus_tag="VFH96_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6973095.1"
                     /translation="MSTTGSYEQFLEDFRDTIVSAKARLSDIPEAQSRQKSSPDDWSP
                     AEVLGHLIDSAANNHQRFVRAQFTNDLVFSGYEQNQWVSSQKYSDESWSDVIQLWSVY
                     NLHLLHVVSVIPEDVLTKARSPHTLDQIAFNLVEKNDPATLEYLIRDYVDHLRHHLDQ
                     IFAEGKQ"
     gene            9053..9712
                     /locus_tag="VFH96_03675"
     CDS             9053..9712
                     /locus_tag="VFH96_03675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6973096.1"
                     /translation="MSTKTVIKAEPEMEAVRLGTMRWVRRFSKLFAVFAVALLLFVVT
                     GASDRLVLYARILKLYTQAPDTRLAMPLEDVRKTAIADTWQAARGVGRKHEGQDIFAP
                     KGTPILSATSGYIYNIGENNLGGQTVSVISKGGRIYYYAHLDSYARGIKMGDRVTTRT
                     VLGYVGTTGNAQGTPPHLHFGVYTSSGAINPLPMLTDRLPPTATRQETRPRSQLINRK
                     A"
     gene            9882..12527
                     /locus_tag="VFH96_03680"
     CDS             9882..12527
                     /locus_tag="VFH96_03680"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET6973097.1"
                     /translation="MRMRSRYLPVSILVITLVISVFLLAPRSEANNSPQDPPTPPAGG
                     TGQERPGRQDQSPEPRPYDRVITKDAKSDEGVFTIHTIKDKVYYEIPKTELGKEFLWV
                     SQIAKTTLGVGYGGQANGNRVVRWERKGNRILLRNIVYDVVADPKLPVSRAVQASNND
                     TIIMAFNIEAIGKDDSAVIDVSRLFTTEVTELSARTRLRARGFDASRSFIEKTKSFPT
                     NIEVEVSQTYTSPPDANPGGGGGPQPQPAPNPFAQGMRAGTNATVVMHYSMVKLPEQP
                     MMPRLFDDRVGYFTVRKTDYGTEEQRAATRTYITRWRLEKKDPNAEISEPVKPIVYYV
                     DPATPTKWVSWIKKGIEDWQPAFEAAGFKNAIIAKEAPSKTEDPYWDPEDARYSVIRY
                     LPSTIMNASGPHVSDPRTGEILESDIQYYHNVMNLARNWYFLQVGPLDARAKKLPLPD
                     DLMGRLVEYVIAHEVGHTLGFQHNMKASATYPAEKVRNAEWVRTMGHTPTLMDYSRFN
                     YVAQPEDNIPVEDLIPKIGPYDKWATMWGYKPIPGARTPDEEKKTLDEWARGQDSKPY
                     LRFSTADARGSDPGENTEAVGDADAVASTTLGIKNLKRVADMLLPATTQTGEPFDDLE
                     ELYNSMLGQWTLELNHVTGIVGGFNSQQKHAGQDGVRFVIVPKERQAAAVRFLNENAF
                     ATPTWAVKADILRRIEPAGALARVNAAQERVLNSLLSNTRFDRLVEQEALDGMAAYKP
                     ADFMSDVRKGIWRELEVGPVQIDVYRRNLQNSYIDLLSTKLNGRPAVTDDYRALIKAE
                     LRDLSVAIAASMPRASDRQTRAHLADARDQIAKALDPKFAAPAPATPPSPFGFDDEFN
                     SVGLEDFDFKDCWPDYSIRIRPRKE"
     gene            complement(12564..13199)
                     /locus_tag="VFH96_03685"
     CDS             complement(12564..13199)
                     /locus_tag="VFH96_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6973098.1"
                     /translation="MYCPQCGTEAQATQYCRTCGANLKVIGKAVALSEAVARSDRGPL
                     PKIKEMIGNFKVDHVSDEVSGALDRMNQEIVRISPETQRRAWIEFPKRKTPAEKRQDH
                     ISKGVVSFFSGIGLTVFLYYLSTVLVLKLPPHIIDQIPFVVDPVVKIIWLLGLLPVTT
                     GIGHIIAGLLIRPQRDEEPKQIDQVVSPPLISITDHTTNLLEPQKAQKAQK"
     gene            complement(13239..>14615)
                     /locus_tag="VFH96_03690"
     CDS             complement(13239..>14615)
                     /locus_tag="VFH96_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973099.1"
                     /translation="QPQVAQAYIIESLTKVINFAFAFVPGTIGVYEGGTEVILQKGLG
                     FTPAAGLALALVRKAAIVVWTSIGLLVLTWRTLPNAWRRILDRSPRLQRLMDSLVLSN
                     IAHRPARTAVSILGTGVGVLLIVFTVGLAHGVLHERGRRESNIGAEIMIRSSGTIGLG
                     GSQFRLPASHAAEIATIPGVRAATPIGQTFDKSDTGFGQRLIDGIEYDQYATIARITM
                     REGRKLESGDEAIIDPEWQRTRKANVGDTVKLFERSFKIVGVYEPPGGGRIKIPLKTM
                     QDQEGSDNKASAILVACANPEQQEEVAARILERFPEDQLIFTRDLPEIYASGVPALNV
                     FIKVVVGVAAAISVLVILLAMYTTVTERTRQIGILKSLGMSKTAIAWVIEQEAIIVSV
                     LGVVVGVLLTMLAKFAVMRTTSLTIDIEPRWVLIALAVGLVGGTIGALYPALRAARQD
                     AVEALSYE"
BASE COUNT         3229 a         4206 c         4138 g         3042 t
ORIGIN      
        1 cgtaaaacgg cagcgaaaac agataaaacc ccagcgactt gccgaagatc ggatcgtgtg
       61 tcgccgttga cggttgattg aagtagagcg cgaactgtcg ccacttctcc ttcaacataa
      121 agccgtaaat cagtccgaaa acaccggaca caacccagcc gagcggccga ataaaccgcg
      181 ccggtgagaa ctgaaacggc tggttgttga gaacgatcgt gcgctgctcg aatgcgtaag
      241 ctccgaagag tcgctggaag agcagaaacg tcgcgctcaa gagtaacgcc gtcaatattg
      301 cagagccgaa gaagagggcg agttttgctt tgaagacgta ccagtagact gatgagtagc
      361 cgagcgatcc aaaccatagc gccgagatat agatcgagac aatccgcgag agcgagaaca
      421 acagcagaat cgccgcgacc acgagccaga ttttccaacg ccgccgtgtg cgtggtccaa
      481 cgtcgatgat ttcgggggct ctggtgtttc ggctcatgcg cgtatcttgc cacagatcaa
      541 cacggattac acggctcgtg tataatcgcg aggatgctca cgccagagac cgttctacaa
      601 ggcaggtatc gtatcgtacg ccagcttggg cagggcggca tgggcgcagt ttacgaagcg
      661 atcgatcagc gcctcgatac cacggtcgcg ctgaaagaga cgcttttttc tgaagaacgc
      721 ctgcgcaaac agttcgaacg cgaggcgcga cttctcgctc gcttgcatca tcccgcattg
      781 ccgcgcgtga gcgaccattt ttccgaagca gacgggcagt ttctcgtgat gcagttcatc
      841 cccggcgacg atctgtcgga gatgatgaat cgcaaacgag gtccttttcc cgcgaaccag
      901 gttttaacgt gggctgatca gttgctcgat gcgctcgact acctgcacac gcaggatccg
      961 cagatcgttc atcgcgacat caaaccgcaa aatctgaaac ttactgcacg cggccagatc
     1021 attctcctcg acttcggact cgctaaaggc caggccggcg agatctcacg cgtgacgacc
     1081 gctgcgagta tcttcggcta cacgcccaac tatgcaccgc tcgaacagat tcagggtttg
     1141 ggaactgatt cacgcagcga tctatattca ctcgctgcga cgctctatca cctgatgacg
     1201 ggcgtgaagc cacctgatgc cctcacgcgc gcggctgcac tcgtgaacgg ccagcccgat
     1261 ccgttgttga aagcgagcga agccaactcg gagatctcgc ctgacattga cgaagtgtta
     1321 gccaaagcga tggcgcaaaa tcgcgagcag cgttatgcga ccgcggctga catgcgacga
     1381 gcgcttcacg aggctgagca ggcgtcgact gtagtgaatc gtggtgaggc gcagacggtt
     1441 ttattaccgg cgccgccacc gaagactgtg ccagtgcaga cccaaacagc cgctaccccg
     1501 cagacggttg gtccaacagg tgagacgacg gttgttcgtc cgaaaggtag tggtccagga
     1561 cgactggtgc cggttgcgat attggccgcg gtgttagtgt tgattggtgg tgcgctcggt
     1621 ttcttcgcac tgaagaagca caaccaacag gacgcgccgg tcgcgacaga gagtccagcg
     1681 ccacttggtg gtccagccgc cgacactcct tcgccgtcac cggaagcaaa aacgtctgcc
     1741 gaacaagctg cggcgacccc taagaaaact gaaaagaccg cgcgtaacga agagccgaag
     1801 agagcgcccg cgcccgagaa gcgtgctgag ccgcgagcgg atgtcgacgc taagggacac
     1861 gatgaagaag atgagccccc ggacacaccc gttccgcccg attttcagag acagaatcga
     1921 ccgcgccggc cgatggtcag agtgatgccg ggcggcgtta agattcagac tttgccagac
     1981 ggtacacaga tcatcacgac gggcgatggc ggacgtttta tgattgggcc ggatgggaag
     2041 cggaccgttc tcaatccagc gcgacctcgt cgccggccag caccggcgcc gacaccagaa
     2101 ccataacaac agccaataaa gaggcaacaa tatgtataaa aggaaaacgt ttagagttcc
     2161 gacgtttgcg ctacttgcac tgtttgcatg tctggcgatg ctgacctcgg ttgatgttgc
     2221 agtgcgggca aagaacgaga gagtgattcc gtccaacatc cgtatcactc cgccggcgcc
     2281 tgcattcgtc gacaaggatc gtgtcgccga gctgaagcag cgacgcgagc gcgtggccca
     2341 gagtattggc ccacagtcgt tcctgattct tttcagcacc gagccgcgcg tctacacgaa
     2401 cgacgtcgat tatccctatc gtcaggaaaa caatctctat tacctcacga acctgaagca
     2461 aaaaggcgcg acactcgtcc tgttgccggg caacacgaag atccgcgaga ttctcttcat
     2521 gccgcgtcgc aatccgggag cggagacgtg gaccggtcac atgtacagcg ctgaagaagc
     2581 gaagcagctt tccggggtca gcgagatctg gcaggcaagc gagtttgagc cgttcatcgc
     2641 ggcactgcgt aatcgacagc cgtaccggcc caagcccgag gccatcttca tgtcggatct
     2701 gccgctcgac atgccgctga acaacgatca tggttacacg cagctatttg acgcggctgc
     2761 caaaggtgaa gcgggactgt tcatgctggt tccgccaggc cagggcgaga atcgtgaata
     2821 caaacaggag caacgctttg cgtccaactg gtccagcacg gcttccggtt acagtgtgaa
     2881 aaatgcgtgg cccatcttca cggagatgcg gctcgtgaag tcgccgatgg agctgcgtct
     2941 tcttcagcac gcgatcgaca tcagcaccga ggcgcaccag cgcgcgtggg ctgccgcagg
     3001 tgaggcgaag tgggagtatg aagtcgatgc ggtgatggct tatacgttca agcttcgcaa
     3061 tgccgataac tggggctatc ccgatatcgt cggctgtggt ccaaacgcga cgacgctgca
     3121 ttacatcgag tcgcagggac aagtgaagca gggcgatctg ctgttgatgg acgtgggcgc
     3181 cgagtacgat cactacacgg cggatatcac gcgcacgttc ccggtgaatg gcaagttcac
     3241 acccgctcag gccgcggtct atcagattgt ttatgacgcg caggaagcgg tagcgaaagc
     3301 gtcgaagccc ggcgcgacgt tgtcagatgt gaatcgtgcg ggtacggaag tggtgaagga
     3361 cgggctattg aaattagggc tgatcacgga tcgcaattcg aaccagcatc gcatctggtt
     3421 catgcacggc acgtcacact ggctgggcat gaacgttcac gatgttggtg gtggcgcgag
     3481 gttcgtgccg ggagtcacgt tcacaaacga gccgggaatt tatgtgcgac ccgacgcgtt
     3541 ggaccagatg ccgtacggtt ggaagcagga agattgggag aagttcaaga ccgcggtgcg
     3601 gcctgcgttt gagaagtaca aaggcatggg cgtgcgaatc gaagacgata tggtgatcac
     3661 tgcggatggc gtgcgctgga tgagcgaagc gctgccacga aagatcagcg acatcgagga
     3721 cttcatcgcc aaagcgcgcc gccaatcaga ataaagccag ccacaaaagg cacaaaaagc
     3781 acaaaagaaa agctaaatca ttttgtgctt tttgtgcctt tttgtggcct aaacaatctt
     3841 ttcaggaacc ctgaggccca cctcagggct gttcgaaaaa agccgtgctg cggcggtggc
     3901 tcacccactg ccgtctttgc ttctgcggcc aggctcgtga agaactgcgt ggccatcatc
     3961 ttcgtcgtgc cctgaatcat gcgttggccc acgctcgcga tcgttccgcc aacttgcacg
     4021 tcgcccgaat agcttacctt cgtgccgttc tctgtttgct cgagatccaa gtctcctgag
     4081 cccttaagaa atccgggttg acccttgccg tcaacaacga tgcgaagatg ttcgggctcg
     4141 cgcagatctt cgagacgcgc agttccgttg aagacaccct tgatcgatcc gactccggcg
     4201 cgaatcgtcg ccgcaaacgt gttctccgcg gtctgttcga gcttttcgca accaggaatg
     4261 cagcgctgta acacctccgg cgtgatgagg cattgataga cgcgctctcg cttggcgtcg
     4321 agctcgtgag tgccttccag tttcactgca cgcgagcctt cgccgtttga atgccgcggc
     4381 gcgtgtggac ctgcgcgaga tgacaccggt aatcgcctga cgcgtagaga tcggagttcg
     4441 gatcgatacc gtcgcagacg tgcgcaactg cggccgcgac cgcgttgtcg tccaaattcg
     4501 ttccggcaag cgccgcttcg acggctttgg cgcgatatgc tttgacgccg acgccagtca
     4561 cgccgatacg agccgacttg cacgagccat cgtcattcaa ctcgagattc acagcgattc
     4621 cgactactgc gaatccagac gcgggatgcg gcactttttg ataagcgtgg ccaaagcgtc
     4681 cgctcggttt cctgatgcga acttcgcgca gtatctcgcc cggctgtaga tcagtcgtca
     4741 gcagatcgac gaaaaagttt tctgctttga cggtgcgctc gccgtttggt ccaacgagaa
     4801 cgaggtccgc atcgagcgcg agcaccgcgg cgggccagtc acccgccgga tcggaatgcg
     4861 caatgctgcc gccaatcgtg cccttgtttc gcacctgaac gtcgccgatg ctcttcgcac
     4921 actcaggcaa cagcggacaa atccgctgca acagatctga cgattccaac tggtagtgag
     4981 tcgtagtcgc accgatcagg atctgatcgc cctcttcacg aatgtaggcg agcgacttga
     5041 tgcgcgcgat atcgacgagc aactgcggtt gcacgagacg cagcttcatt gccggaatca
     5101 gactgtgccc accggcgagc accttcgcgc catcgagatc gctggcgagc aacgcgactg
     5161 cttcagcgag cgtcgcgggc gtcttgtagt cgaattgcac tggaatcatg atctgctcct
     5221 aatgtccatt gatgatccgc caaatgttct ccggcttgac cggcatatca agatgcttca
     5281 cgccaaacgg cgcgagcgca tcgacgatgg cattgacgat cgcgggcgtc gctccgatcg
     5341 ttcctgcttc gcccacgcct ttgaccccga gtggattgac cggcgatggc gtctctgttc
     5401 gatcgagctc aaactccgga acgtgcgacg cgcgcggcaa cgcatagtcc atcaaagttc
     5461 ccgtgattag ctgtccctgc tcgtcgtaca caacctcttc gaacatcgcc tgtccgatcg
     5521 actgcacgat gccgccgtga atctgcccgt caacaagcat cggattgatc acgcggccgc
     5581 aatcgtcaac cgcgacataa cgcagaaact cgatgtcgcc ggtgtcgcga tcgacttcga
     5641 cgacagcgat gtgtgtgccg aacgggaacg tgaagttctt cggctcgaaa aagtacgttg
     5701 ctgagagacc cggctcggtg tcgggcggca gctctttcgc gaggtgcgcc gcgagggcga
     5761 tgtcctggat tgttagactt gtcttgcctt cggtctgcgg ctctggcagc gcgccggctg
     5821 gcgcctcgcc gacaggcaca acgggatccg acgttccggt tgctgccgtt gcggccgcgg
     5881 ctttctcgag tgaatacttt ccaccttcga atgcgacatg cgaagcatca gccttgagca
     5941 tgtgagtcgc gatcttgtgc gctttctcct tcagtttttg aatcgcgaca aagacggccg
     6001 tgccgccgac agcagtcgcg cgactgccaa acgtgccgat accgtattgc acgattcccg
     6061 tgtcgccgtg gatcacggtc acgtcgttga ggttcactcc cagctcatca gcggcgatct
     6121 gtgcgaacga agtttcctga ccctggccat gcggcgacgc gcccgtcatc accgtgacct
     6181 ttccagtcgg ctcgatacgt accgtcgcgc tttcccaacc accggcgggc atcgcctgcg
     6241 atggaccaag cgcgcaaatc tcgacgtaag tcgagagacc gataccgatc aaacgtcctt
     6301 cctcgcgcgc cttcctctgc ttatcacgca ggtcagcgta acccgcgatc tcctgtgctt
     6361 tgttgagcgc tgtttcatag tcgccgctgt cgtagtcgag accagtcgct gtgtggaatg
     6421 gaaactcgtc agcagcaggg aagttcttgc gacgcaattc cgcgacatca acgccgagat
     6481 caaccgcaac cagatcgagc gcgcgctcga cgacgtacgt tgcttcagga cgtcccgcgc
     6541 cgcgatatgc atcggtggcc atcttgttcg tgaagcaacc gacgatgttc atctgaatcg
     6601 cgggaatctt gtaagcgccg gagagcatga gcccggtcaa cgttggaatc gcgggcgtca
     6661 gcagttgatg ataagcgccg aggtccgcga agacgctgta acgcaaaccg agcagcgtcc
     6721 catcgttctt gaacgcgact tcgatatcac cgacctgacc acgaccgtga atcgtcgccg
     6781 ccatattctc gcgacgtgtc tcgatccatt tgacgggttt gccaagctgg atcgaaatcc
     6841 aactgagcag cgcttcttcc gcgtagacgt tcaacttcga tccaaaaccg ccgcccactt
     6901 ccggagtaat cacgcgcaac ttgttttcgg gcattccaat catgagtgcg acttgcgtac
     6961 gcatgaggtg cgggatctgt gtcgacgacc agagcgtgag ctcttcttca cccggaaaat
     7021 agcgggcgag cacgccgcgt ggctccatcg cgatcggtgc gagtcgtctg tgcagaatgc
     7081 gttgtttgac ggttcgatca gcggcggcga aagcggcatc gatatcacct tcaccggagg
     7141 ttagcttgta agcaatgttg ctgccgaacg cttcgtggat gactggacca ccagcggccg
     7201 cttgttcgac gtcagttacc acaggcagat cttcgtaatc gaccatcacg agatcgacgg
     7261 cgtctcgcgc agcgtaacga tccttcgcca cgacggctgc gatcggatgg cccacgaagt
     7321 aaaccttgtc tttggccaac acacgatgat cgggaacttt caattccggc aacgcactcg
     7381 cgcacggcac cggaccgatc ttttccgcga catcgtgtcc ggtataaaca gctacgacgc
     7441 ccggagcagc gagtgcggcc gacgtatcga tgctgttgat ctttgcgtgc gcgtacatcg
     7501 agcgaacaaa cgcgacgtgc aatgtgccgg gtaacgcgat gtcgtcgacg tagtgtgcga
     7561 ggcctcggat taaacgcgga tcctcggtgc gctttactcg tttcccaacg tagttctccg
     7621 tcataagact cctcccatca acacgaatcc atcagtacaa gatccgtgtc tatctgtggc
     7681 ccgctttctt cgccgcgtac tggaccgctt cgatgatgtg ctgatatccg gtgcagcgac
     7741 acagattgcc ttcgagcccg tggcagatct cttcacgcga aggatcggcg ttccggtcga
     7801 gtagctgcgc cgccgtcatg atcatgcccg gcgtgcagta gccgcactgc aatccatgtc
     7861 tttcccagaa accttcctgc accggatgca gctcgccatt cttcgccagt ccttcgatcg
     7921 tcgtgactga cgagccgtcg gcctgcacgg caaacacggt gcacgactta actgcctgcc
     7981 cgtcgagcat cacggtgcac gcaccgcaca acgacgtttc acaaccgacg tgtgtgccgg
     8041 tcagctccag cacgtcgcgc agataatgga caagcagcat acgcggctcg acctcatcgg
     8101 tatgagagac accgttcacg ttgagattga tagccttttt catgagctac ctcactttca
     8161 attctgtaga tattaaaccg tggctttgtg aatccgtcgc ttcagacgat gaatggtccg
     8221 gcggacggtc ttgagctgtg cgcgatcgtg cgcttcgatt gcggctgcgc gctgtgtttt
     8281 caactcacga atgcgcgctt tgatcgagct cttgtcgata ccgacaacgg agtggtgctc
     8341 gtgttgaatg ccgagcgctt tggctagcgc caccgcgaga tgctctttgt tcatctgcgt
     8401 gtatccctga acggcttcgt gttcgttgcc tttcgccatt tcgcgcagtt ccgcgagtgt
     8461 cttgtgtctc agttgttcgt atgtcaactc cgccattgga agctcctatt gtttaccttc
     8521 tgcaaagatt tgatcgagat gatgtcgcag gtggtccacg tagtcgcgga tcaggtattc
     8581 gagtgttgcc gggtcatttt tctcgacgag attgaatgcg atctgatcga gcgtgtgagg
     8641 cgagcgtgct ttagtcaaaa cgtcttcggg tatcactgaa acgacgtgta gcaggtggag
     8701 gttgtacacg ctccagagtt gaatcacatc agaccacgac tcgtcgctat acttttgcga
     8761 gctgacccac tggttctgct cgtaacccga gaacacgaga tcgttagtaa actgcgcccg
     8821 tacaaaacgc tggtgattgt ttgcggctga gtcgatcaga tggccgagaa cctcagccgg
     8881 agaccagtcg tctggagaac tcttctgccg gctctgtgct tcgggtatgt cgctcaagcg
     8941 cgctttcgca ctcacaatcg tgtcgcgaaa gtcctcgagg aactgttcgt agctgcctgt
     9001 ggtggacatg gaaactggat aatatcacgt caatcctgaa aatcctgtca tcttgtcaac
     9061 gaaaaccgtc atcaaagcgg aaccggaaat ggaggcggtt cgtttaggta ccatgcgctg
     9121 ggttagacgt ttttcaaagc tttttgcggt gttcgcggtc gcgttgctgt tattcgtggt
     9181 gaccggcgcc tcagacaggt tggtcttata cgcacgcatt ctaaaactct acacgcaggc
     9241 gcctgatacg aggctcgcca tgccgctcga agacgtcagg aagacggcga ttgcagacac
     9301 atggcaggcg gcacgcggtg taggacgcaa gcacgaaggt caggacatct tcgcaccgaa
     9361 ggggacaccg atcctctccg caaccagcgg gtacatctat aacatcggcg agaacaatct
     9421 tggcggacag acagtttccg taatcagtaa gggcggccgg atttactact acgcacatct
     9481 cgattcttac gcgcgaggaa tcaagatggg cgatcgggtg acgacgcgaa cagttctcgg
     9541 atacgtcggc accaccggaa acgcacaagg cacgccgcca catctccact tcggtgtgta
     9601 cacctcgagc ggcgcgatca accctctgcc aatgctgact gatcgcctcc caccgacagc
     9661 gacgagacaa gaaaccagac ccagatccca actcatcaac cgcaaagcct gatcttgcag
     9721 atgtccgaca tgctttagct tgtcgggatt gcgcgacaaa ctgaagtttg tcggacacta
     9781 cctacgcttg tgccttttgg tggcatcggc tagacttttc gcgctttctg ttcacagttc
     9841 gccagccata acttaatacg ccaatcggaa ggagctaacg catgcggatg cgatctcggt
     9901 atctccctgt ctcgatcctt gtaataactc tcgtcatctc cgtctttctc ttagcgccgc
     9961 ggtcagaggc gaacaactcg cctcaggatc caccaacacc accagccggc ggcacgggtc
    10021 aggaacgtcc cggccggcaa gatcaatcgc ctgagcccag accgtacgac cgtgtaatca
    10081 cgaaagacgc aaagtctgac gaaggcgtct tcacgatcca cacgatcaaa gacaaggtct
    10141 attacgaaat cccaaagacc gagctcggca aggagtttct ctgggtcagc cagattgcca
    10201 agacgacgct cggtgtcggc tacggcggcc aggctaacgg caatcgagtc gtgagatggg
    10261 agcgtaaggg caatcgcatt ctgctgcgca acatcgtcta cgatgtggtc gccgatccga
    10321 aactcccggt atcgcgagcg gtgcaggcct caaacaacga cacgatcatc atggccttca
    10381 atatcgaagc gatcggtaaa gacgactcgg ccgtgatcga cgtgtcgcga ctgttcacga
    10441 cggaagtcac cgagctcagc gcgcgtacac gtttgcgtgc tcgcggtttc gatgcgtcgc
    10501 gctcattcat cgagaagacg aagtcgtttc cgacgaacat cgaagtcgaa gtttcacaaa
    10561 cctacacctc gccacctgat gcgaaccccg gcggcggcgg tggaccacaa ccacaacccg
    10621 cgccgaatcc gtttgcgcag ggcatgcgtg caggaacgaa tgcgaccgtc gtcatgcact
    10681 acagcatggt gaagcttccc gagcagccga tgatgccgcg actttttgac gaccgcgtcg
    10741 gctatttcac cgtgcgcaag actgactacg gtaccgaaga gcaacgcgcc gcaacgcgaa
    10801 cgtacatcac gcgctggcgt ctcgagaaga aagatcccaa tgctgagatc tccgaaccgg
    10861 tgaagccgat tgtgtactat gtcgatccgg ccacaccgac caaatgggtt tcgtggatca
    10921 agaagggaat cgaagactgg cagcctgctt ttgaagccgc tggtttcaaa aacgcgatca
    10981 tcgcgaagga agctccgtcg aagactgaag atccatactg ggaccccgaa gatgcgcgct
    11041 attcagtcat tcgttacctg ccgtcaacta ttatgaacgc ctccggcccg cacgtgagcg
    11101 atccgcgcac tggtgaaatt ctcgagtcgg acattcagta ctatcacaac gtgatgaacc
    11161 tggcgcgcaa ctggtacttc ctgcaggttg gcccactcga tgcgcgtgcg aaaaaactgc
    11221 cgttgccgga cgatctgatg ggccgtctgg tcgagtatgt gatcgcgcac gaggtcggac
    11281 acacgctcgg ttttcaacac aacatgaagg cgagtgccac ctatcccgcg gagaaggttc
    11341 gcaacgcgga gtgggtcagg acgatgggcc acacgcccac cctcatggac tattcacgtt
    11401 tcaattacgt ggcccaaccg gaagataaca ttccggttga ggacctgatt ccaaagatcg
    11461 gtccgtacga caaatgggcc acgatgtggg gctacaaacc gattccaggc gccagaacgc
    11521 ccgatgaaga aaagaagacg cttgacgaat gggcgagggg acaggattca aaaccgtatc
    11581 tccgcttctc cacagcggat gcacgcggtt ccgatccggg tgagaacacc gaggcggtag
    11641 gcgatgccga cgcggtggcg tcaaccacgc tcggtatcaa gaacctgaag cgcgtagccg
    11701 acatgctgct gccggcgacg acgcagacag gcgagccttt cgacgatctc gaagagctat
    11761 acaacagtat gttgggccag tggacactcg agttgaatca cgtgacgggc atcgtcggtg
    11821 gcttcaactc gcaacagaaa cacgccgggc aggatggcgt acggtttgtt atcgttccga
    11881 aagagcgaca ggccgcagcg gtacgcttcc tgaacgagaa cgcgtttgct acgccaacgt
    11941 gggcggtcaa ggctgacata ctgcgacgca ttgagccggc cggcgcgctc gcacgcgtga
    12001 atgcagcgca ggagcgggtt ctcaattcgc ttttaagtaa cacgcgcttc gatcgtttgg
    12061 tcgagcagga agcactcgat ggcatggctg cgtacaagcc ggcagatttc atgtcggacg
    12121 tgcgcaaggg tatctggagg gagctcgaag ttggaccagt tcagatcgat gtctatcgtc
    12181 gtaaccttca gaactcgtat atcgatttgc tttcaaccaa gctgaacggg cgaccggcag
    12241 tgactgacga ttatcgtgcg ttgatcaaag cggagttgcg cgatctgagt gtggccattg
    12301 ctgcttcgat gccacgtgcc tcagatcgac agacgcgcgc gcatcttgcc gacgctcgcg
    12361 atcagattgc gaaggcgctc gatccgaagt ttgccgcgcc tgcgccagcg acgccgccaa
    12421 gtccgtttgg atttgacgat gagtttaatt ccgtgggttt ggaagacttc gatttcaaag
    12481 actgctggcc ggattactcg atccggatca gaccacgaaa agagtaagaa ttagccacaa
    12541 aaaggcacaa aaagcacaga agtttatttt tgtgcttttt gtgccttttg tggctcaaga
    12601 aggtttgttg tgtgatcggt gatgctgatc agtggtggtg aaacgacttg atcgatttgc
    12661 ttcggttctt catcgcgctg cgggcggata aggagtccgg cgatgatgtg gcctatgccg
    12721 gttgtcactg gtaacaaccc aagcagccag atgatcttca ccaccggatc gactacaaac
    12781 ggaatctgat cgatgatgtg gggcgggagt ttcagtacga gcacggttga aagatagtag
    12841 agaaagacgg tcaagccgat gccggaaaag aacgagacca cacctttcga gatatgatcc
    12901 tggcgtttct ctgccggtgt ttttcgtttc ggaaattcga tccaggcgcg gcgttgagtc
    12961 tcaggcgaga tgcgcacaat ctcctggttc atgcgatcga gcgcgcccga aacttcgtct
    13021 gacacgtgat cgaccttgaa gttgccgatc atctctttga tcttcggcag agggccgcga
    13081 tcgcttcgcg caacagcctc gcttagcgcc accgctttcc caatcacctt cagattcgcg
    13141 ccgcaggtgc ggcaatactg cgtcgcttgc gcttctgtgc cgcattgagg acagtacatg
    13201 ttctcgaaac gtcccggaag tcagtttggt tccaacacct attcgtagct caatgcttcg
    13261 accgcatcct gccgggctgc gcggagggcc ggatacagcg cgccgatggt gccgcccacc
    13321 aatccaacgg cgagcgcaat caacacccag cgcggctcga tgtcgatcgt taaactcgtc
    13381 gttcgcatca ccgcgaactt cgccagcatc gtcagcaata caccaactac gacgccaagc
    13441 acactgacga tgatggcctc ctgctcgatc acccacgcga tcgcagtctt cgacatgccg
    13501 agtgacttca gtattccgat ctgtctcgtg cgctccgtca ccgtcgtata catcgcgagc
    13561 agaatcacga gcacactgat cgccgccgcc actccgacca cgaccttgat gaacacattc
    13621 aacgccggaa caccactcgc ataaatctcc ggcaggtcgc gcgtgaagat caattgatcc
    13681 tcaggaaaac gctcgagtat gcgcgcggcg acttcttcct gctgctccgg attcgcacac
    13741 gcgacgagaa ttgccgacgc cttgttgtca gagccttcct ggtcctgcat tgttttcagc
    13801 ggaatcttga tacgaccgcc acccggcggc tcgtaaacac cgacaatctt gaaagaacgc
    13861 tcgaaaagtt tcaccgtgtc gccgacattc gccttgcggg tcctttgcca ctccggatcg
    13921 atgatcgcct cgtcgcccga ctcgagtttc cgtccttcgc gcatcgtgat gcgcgcgatg
    13981 gttgcatact ggtcgtattc gattccatcg atcagccgtt ggccgaaacc cgtgtcgctc
    14041 ttatcgaacg tttgcccgat cggtgtcgcc gctcgaacac cgggaatcgt tgcgatctcc
    14101 gccgcgtgtg acgcgggcag tctgaattga gaaccgccca gaccgatcgt tccggaggag
    14161 cgaatcatga tctcggcgcc gatgtttgat tcgcgccggc cacgttcatg caaaacgcca
    14221 tgagcgagac cgacagtgaa gacaatgagc aacactccga caccggtgcc gaggatgctg
    14281 acagccgtgc gagcaggtcg atgagcgatg tttgaaagta cgagactatc cataagacgt
    14341 tgtaaacgcg ggctgcgatc aaggatgcga cgccacgcgt tcgggagcgt gcgccaggta
    14401 agaacgagga ggccgataga cgtccagaca acgatcgcgg ccttgcgcac gagagcaagc
    14461 gcgagaccgg ccgcgggcgt gaagccgagt cctttctgaa gtatcacttc agtgccgcct
    14521 tcgtagactc cgatcgttcc ggggacaaat gcaaaagcga agttgatgac ctttgtgagc
    14581 gactcgatga tgtaagcctg cgccacctgt ggttg
//
LOCUS       DASSFQ010000109         4937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1533423, whole genome shotgun sequence.
ACCESSION   DASSFQ010000109 DASSFQ010000000
VERSION     DASSFQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533423"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFH96_03695"
     CDS             complement(<1..358)
                     /locus_tag="VFH96_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6973100.1"
                     /translation="MELEQVAPATMADARPQVEDPFIERLKRGDSAAFEKLVNERSGE
                     IYGLLYRLTENSEEARDLTQETFLRAFQSINHFRGESDLRTWIYRIAINQARNRWRWW
                     RRRRREVTVSIDEPEIG"
     gene            686..1492
                     /locus_tag="VFH96_03700"
     CDS             686..1492
                     /locus_tag="VFH96_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973101.1"
                     /translation="MAKYKKKRARELQHDRFRDTTMSFFDRLGDLLEGRGRMILYGLI
                     GLIVAALVVFQIVRWTNKKSDEARQALGRAITISRAQVTTTPPPGNTDPTFATDKDRA
                     QRAIEEFQKVAAKYGDPYKSESQYFIASNRLVVDRQKGISELTELQNSKVGEVAALSK
                     FALGQAYESDGKLDDAARLYTELAKLNSTTVTPDTANLRLAKVYTKQGKKKEAADLLF
                     NIVDASRKAKGSDNQPLPQTSAAREAADELQKLDPDRYGQLPPETPLLAG"
     gene            1577..2704
                     /locus_tag="VFH96_03705"
     CDS             1577..2704
                     /locus_tag="VFH96_03705"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET6973102.1"
                     /translation="MDNLTHSLVGLTAAKAGLEKLSPGATTLCIIAANAPDSDIVVLA
                     FGDRWTFLQHHRGITHAIVGVIALAIILPLIFYGVDRLWSRYKEQPPQTKLKGLMIAS
                     FVASATHPLLDWTNNYGIRFFLPWSQQWSYGDLVFIVDPYIWLILGGAIFLLTAKTRF
                     MKVIWGVIAALTTFLVVASPRSGGLPYPNLIALLWIVTIVVFIVLSIKGARERWGKNI
                     AYVAIALVLCYWSLLAFAHSRAVARGSEEAAKMANGETVLRLAAMPRLANPFRWDCVF
                     ETDRAMYRFELGLLTGDGTGNPVRYEKPGPELKPVVDYVSQQRSGRVFLGFARFPVLK
                     LADPGCTTRTLVQLADLRYTEPGRSRGTFSLELPIDCPVAR"
     gene            2701..4119
                     /locus_tag="VFH96_03710"
     CDS             2701..4119
                     /locus_tag="VFH96_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973103.1"
                     /translation="MSETKELDTLPVLSEVSEERERARKKPNIASVRVSPGPYLALAG
                     VVTFISALLLRINYDAAALLMIAGAWLIIPVLALSDRIAFDSTSLRRQGPLATFLHLF
                     FGYRKQLSIDDFETVETQAVRTLRRGGRVRYRYRTQITGKGREFVIVSGGREYRKLVR
                     ELFPLIHESKLDNRSRDLRDYLNDPNFLNRKTQLSQLASSDLLDVTGPDFRLGRKFRR
                     RADVAETPATPEDLERARLLRRLGNELRVSGRLREASEAFRRAMNVRPHGAWLIYDFA
                     RLLRSQASAQADARLLSRARAALRLASIRAENDTVLLPLIGESFLECGDARHARRALE
                     RVVEIDSGNFKARVGLADIALREGKLAHVIHHYQEAARVSSEQSLARYARGEGEYYMQ
                     LNNDDDYLAAELRRINWLQNITRIRRLALRVTNASVLLALIGGLIDPLAGSLGWSLAS
                     SALIIWLLSLAGTHVLFERSKP"
     gene            complement(4187..>4937)
                     /locus_tag="VFH96_03715"
     CDS             complement(4187..>4937)
                     /locus_tag="VFH96_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973104.1"
                     /translation="GKKLGVAIPKTVLLPQKDYIDGIVSESLRNLAFPLDWQGIIDYV
                     GLPAFMKPFDGGGWKNVSKINTLEELWAEYDKTGTLCMTLQEAIEFDQFVRCYCVGQQ
                     EVMIMAYDPRKPYLSGEQYVHDPNYLAPALADRVAKDVRTLCTALGYDINTVEFAIKD
                     NVPYAIDFMNPAPDAELASVGEFYHNWISNAVVDLVFKRLSEGRQQSRYRWDALLNPE
                     PVQAYAVASAAEQQARTVTPAPKKAASKKRK"
BASE COUNT         1092 a         1386 c         1325 g         1134 t
ORIGIN      
        1 cgcctatttc gggctcgtcg atggagactg ttacttcgcg acgccggcga cgccaccacc
       61 gccagcgatt gcgtgcctgg tttatggcga tccggtaaat ccaggtgcgc agatctgact
      121 cgcctctgaa gtggttgata ctctgaaatg cccgcaggaa tgtttcctgg gtcaggtcgc
      181 gtgcttcttc ggagttctca gtcagacgat agagcagacc gtaaatctca ccggagcgtt
      241 cgttcaccag cttttcaaag gcagccgagt cgcctcgctt tagtctttca atgaacggat
      301 cttcaacctg tggccgcgca tctgccatgg tcgcgggagc aacctgctct aattccattt
      361 gcccgtgaag ggcaattggt tctccgtagc tcactgatca ccccctgggg cgggaatcag
      421 agcagttaaa aatgtttgcc ctttcccgga caggcttccc cttagacaac cgtctttcgc
      481 gttttgttcc cgatgttctg gaaaaaaatc cattcgtgct ctctttagcc acagattaca
      541 cagattagat gtatgaatct gtgtaatctg tggatagtgg cttcttgaca atattttgca
      601 cagcccatta gagtgacact ttttccgcgt aatcatacac ccaagagaac gcagccgacc
      661 acgtaggctt gcaagaggat aataagtggc aaagtataag aaaaaacgag cgcgtgagct
      721 ccagcatgac cgcttccgcg acacgaccat gagcttcttc gaccgtctgg gcgacctgct
      781 ggagggtcga ggacgcatga ttctatacgg tttaatcggg ctcatcgtcg ccgccctggt
      841 ggtctttcag atcgtcaggt ggactaataa gaagagcgac gaagcacggc aggcgctcgg
      901 tcgcgcgatc accatttcgc gcgctcaggt tacgacgacg ccacctcccg gcaacacgga
      961 tccgaccttc gccaccgaca aagaccgcgc tcagcgcgcc atcgaggagt ttcaaaaggt
     1021 cgcagccaaa tacggtgacc cgtataaatc ggagtcacag tacttcatcg cttcgaaccg
     1081 cctcgtcgtc gatcgtcaga agggaatcag tgagctgacg gagctgcaaa acagcaaggt
     1141 cggggaggta gccgcgctct ccaaattcgc cctcgggcaa gcctatgaga gcgacggcaa
     1201 gctcgatgat gcggcgaggc tttacaccga actagccaag ctcaatagca caaccgtaac
     1261 tcctgacact gcgaaccttc ggctcgcgaa agtttatacg aagcagggaa agaagaaaga
     1321 ggctgctgat ctgctgttca atatcgtaga cgcatcgcga aaggccaaag gttcggacaa
     1381 tcaacctctg ccacagacgt cagcagcgcg tgaagcggcg gacgagttac aaaaactcga
     1441 tccggatcgt tacggacagc tcccacccga gacaccattg ttggcgggat aaaattacag
     1501 ccacaaaagg cacaaaaagc acaaaagaac taaataaagc ttttgtgctt tttgtgcctt
     1561 ttgtggcaaa gactatatgg acaatctgac tcattcactg gtcggactga ctgcggccaa
     1621 ggctgggttg gaaaaattgt ctcccggcgc gaccacgctg tgcataatcg ccgccaacgc
     1681 acctgattca gatatcgtcg tcctcgcctt tggcgatcgc tggacgttcc tgcagcatca
     1741 tcgtggcatc actcacgcga tcgtcggtgt gatcgctctc gcgatcatct tgccactaat
     1801 cttctacggc gttgaccggc tctggtcgcg gtacaaagaa caacctccac agacaaaact
     1861 taagggactg atgatcgcct cgttcgtcgc gagcgccaca catccactac tcgactggac
     1921 caataactac ggtattcgtt tctttctgcc gtggagtcag cagtggtcct acggcgatct
     1981 cgtcttcatc gtcgatccgt atatctggct catcctcggc ggcgcgattt ttctgttaac
     2041 tgcgaagacg aggtttatga aagttatctg gggcgtgatc gcggcgctca cgacgtttct
     2101 cgtggtggcg agtccgcgca gcggtggctt gccatatcct aatttgatcg cattgttatg
     2161 gatcgtgacg atcgtcgtgt tcatcgttct atcgatcaaa ggtgcgcgag agcgttgggg
     2221 aaaaaacatc gcttacgtcg cgatcgcact cgtgctttgt tactggagct tactcgcctt
     2281 tgcacattcg cgagcagtgg cgcgtggcag tgaagaagcc gcgaaaatgg cgaacggcga
     2341 aacagtatta cgtctcgcag ccatgccgcg actcgcgaat ccgtttcgat gggactgcgt
     2401 gtttgaaaca gaccgcgcga tgtaccggtt tgagctcgga ttgctcaccg gcgacggaac
     2461 cggaaatccg gttcgttacg agaagccagg acctgaattg aaaccagtgg ttgattacgt
     2521 ttcgcaacag agatcgggcc gggtatttct gggcttcgca cgatttcccg tattaaaact
     2581 cgcagatcct ggatgcacta cccgcactct cgttcaatta gccgacttgc gctacactga
     2641 gccgggcaga tcacgcggca ccttttcact tgagttacca atcgattgcc cggttgcaag
     2701 atgagcgaga caaaagaact cgatacccta cccgtcctct ccgaagtaag cgaagagcga
     2761 gagcgcgcgc gaaagaagcc aaacatcgcg agcgtacgcg tctcgcccgg tccatatctc
     2821 gcactcgcag gcgtagtcac attcatttcg gcactcttgc tgcgtatcaa ttacgatgcg
     2881 gctgcgctgc taatgatcgc cggggcctgg ctgatcattc ccgtgctcgc actcagcgat
     2941 cggattgctt tcgacagcac gtctctgcga cgccagggcc cgctcgcaac gtttcttcat
     3001 ttattttttg gttaccgcaa acagctttcg atcgacgact ttgaaactgt cgagacgcag
     3061 gcagtccgca cactgcgtcg tggcggacgc gttcgctatc gctatcgcac gcagatcacg
     3121 ggcaagggtc gagagtttgt gatcgtgtcg ggcggtcgtg agtacagaaa actcgtgcgc
     3181 gagttgttcc cgctgatcca tgaaagcaaa ctcgacaacc gcagtcgcga cctgcgcgac
     3241 tatctcaacg atccgaattt cctgaaccgc aagactcagc tatcacaact cgcttcatcg
     3301 gatctgctcg acgtgacggg accggatttc agacttggtc gcaaattcag gcgacgagct
     3361 gacgttgccg agacgcctgc gactcctgaa gatctcgaac gagcacgact gctgcgtcgc
     3421 ctcggaaacg agttgcgcgt ttcgggtcgc ctgcgcgaag caagcgaagc atttcgtcgc
     3481 gccatgaacg tccggccaca cggcgcctgg ctgatttacg acttcgcccg tttactgcga
     3541 tcgcaggcga gtgcacaggc agatgcgcga ctgttgtcgc gtgcacgtgc ggctttgcga
     3601 ctggcgagta ttcgcgccga aaacgatacc gttttgttgc ctctgattgg cgagagtttt
     3661 ctcgaatgtg gcgacgcgag acacgcgcgc cgtgcgttgg aaagagtcgt cgagattgat
     3721 agcggcaact tcaaagcgcg tgttggtctc gctgacattg ctctgcggga agggaagctc
     3781 gcccacgtta ttcatcatta tcaggaagcc gcgcgtgtga gctcggagca gtctttggcg
     3841 cgttacgctc gcggcgaagg cgagtactac atgcagttga ataatgatga cgattatctc
     3901 gccgccgagc tgcgacggat caactggctg caaaacatca ctcgcattcg tcgactggct
     3961 ttgcgggtga cgaatgcgag tgtgttgctt gcgttgattg gtggtcttat cgatccgctg
     4021 gcgggaagtc tcggctggtc gctggcctca tccgcactga ttatctggtt actcagtctc
     4081 gccggcactc acgtgctctt cgagcgctcc aaaccttaga caggatgcac aagatttttc
     4141 aggattaaca tgtaaatctt gaaaacatcc tgttaatctt gtctaactac tttctctttt
     4201 tcgaagcggc cttcttgggc gcgggtgtaa ccgttctcgc ctgttgttca gccgctgacg
     4261 ccaccgcgta agcctgcacc ggctcgggat tgagcaacgc atcccaacga tagcgcgact
     4321 gctgccgtcc ttcactcaat cgcttgaaca ccagatcgac aaccgcattc gaaatccagt
     4381 tgtgatagaa ctcgccgact gacgccagct cagcatcagg tgccggattc atgaaatcga
     4441 tcgcgtacgg cacgttatct ttgatcgcaa actcgactgt gttgatgtcg taaccgagcg
     4501 ccgtgcagag cgtgcgcacg tctttcgcaa cccgatcggc aagcgcaggc gccagatagt
     4561 ttggatcgtg tacgtactgc tcgccggaca aatacggttt gcgcggatcg taagccatga
     4621 tcatcacttc ctgctggcct acgcagtaac acctgacaaa ctggtcaaac tcgatcgcct
     4681 cttggagcgt catgcaaagc gtgccggtct tgtcatactc ggcccagagc tcttcgagtg
     4741 tgttgatttt cgaaacgttc ttccagccac cgccgtcaaa cggcttcata aacgccggca
     4801 gaccgacgta atcgatgatg ccctgccagt cgagcggaaa cgcaaggttc cgcaggctct
     4861 cgctcacgat gccgtcaatg taatccttct gcggcagcag cacggtcttc ggaatcgcga
     4921 cgccgagctt tttgccg
//
LOCUS       DASSFQ010000110         6194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1984238, whole genome shotgun sequence.
ACCESSION   DASSFQ010000110 DASSFQ010000000
VERSION     DASSFQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984238"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VFH96_03720"
     CDS             <1..1186
                     /locus_tag="VFH96_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6973105.1"
                     /translation="KYTVRLYLTQQAPFFLGLLAHQVFRLVPRKAVEQWGEQWTQPEH
                     IVTCGPFKLKEWVPYDRLVLERDPMYWDAKTVKLDELQFFPILDNATVMNLYKVGELD
                     AVYNHAVPTPWLHILKQKKDYMDRAEAANDYLIMNTTKPPLDNPRIRKAFNLAIDKEA
                     YARWRRIVKPLTAFTPEGTFAGYPQPKGPAFNPAEARRLLGEVGYPVVQNSDGSFSCP
                     KFPVDQVEFIYNSQAANKSVAEWMQAQWKQNLGITVGLRNMDWKTFLEARGKLEYKGF
                     ARGAWGADYMDPFTFLSIFYSAEESGTGWLDPKYVAMLDEANRTLDHKKRYELLAKAE
                     AYMLDNQPIIPIDTPSVNWLKKPYVKGMYPNPASLFAWKYVYIERDRAKWDYGTPSLT
                     AE"
     gene            1215..2141
                     /locus_tag="VFH96_03725"
     CDS             1215..2141
                     /locus_tag="VFH96_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6973106.1"
                     /translation="MFRFIVRRLLVTIPTILVVISITWVLIKLAPGNFYSGEKPLPPA
                     VEKIIREKYGLDKPWYQQYGKTIWGIVRHLDFGTSLRYEGQSVNSIIKRSLPVSATVG
                     ILAYLLALIVGVTIGCIAALKQNSKWDYGSMALAMLGISVPNFVLGPILVLIFSLGLY
                     WLPPSRWGGFPSRNLVLPVLTLSALYMAYIARLTRAGMLEVLRSDYIRTARAKGLSER
                     DVVIRHALRGGLMPVISYTGPALAFLLTGTVVVERIFALPGLGNYFIQASLSRDEPLI
                     IGIVAFIAITLLLMNLLVDIAYAYLDPRVRYQ"
     gene            2150..3166
                     /locus_tag="VFH96_03730"
     CDS             2150..3166
                     /locus_tag="VFH96_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973107.1"
                     /translation="MATEKKPEPDDLIHIGPEMAVAEADLMPGEVGRFVHGTSLWRDA
                     WRRLLKNKLAVFGLVVVVVVTISSIIGPTVIQRVFGFTPDYIPSNDPRLTQSFPPFTG
                     PNGEFSWAHPMGTDNAGRDQFARVLQGGQISLFVGILATLISLLIGVSYGAIAGYVGG
                     RVDNVMMRIVDVLYSLPYVIVVIVLLSMFRSQTPRGQLILLFVAIGTVSWLTMARIVR
                     GQVLSLKNQEFVLAARATGVSNARIIFRHIVPNTLGPVIVYATLEIPQIMLTEAFLSF
                     LGFGVQAPLASWGSLAADGIQNIAVFPWQLIFPGVTMALTLFSLNFLGDGLRDALDPQ
                     MRTI"
     gene            3200..4213
                     /locus_tag="VFH96_03735"
     CDS             3200..4213
                     /locus_tag="VFH96_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973108.1"
                     /translation="MTSSANGNLLEVKNLRTYFETDDGIVKAVDDISFHLKRGETLGI
                     VGESGSGKSVTNLSIIRLVPTPPGKIVSGQVFFNGNDLLHLSNDAIRKIRGRRIAMIF
                     QDPMTSLNPFMKISKQLMEMTELHLGHTKAQAHAHAIRMLETVGIPDARERADSYPHE
                     FSGGMRQRAMIAMALSCEPELLIADEPTTALDVTIQAQILELIKKLKRETGTSVILIT
                     HDLGVVAGMTDHVIVMYAGKIFEQAPTKELFSIPGNPYTKGLLRSVPDPTAEQGKLYQ
                     IPGLPPDVAHLKPGCPFAQRCDYTQDICTREFPPFVQLNADHYSLCHFAREVYSESGG
                     TPV"
     gene            4210..5259
                     /locus_tag="VFH96_03740"
     CDS             4210..5259
                     /locus_tag="VFH96_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6973109.1"
                     /translation="MNSTLLSIRDLKVHFDLGGGGPLDKLFGTNPEKRVVKAVDGVSL
                     DIYEQETLGLVGESGCGKSTLGRAILRLTEPTEGQVFYRDRDLAHLSQRQLREQRRHL
                     QIIFQDPYASLNPRMTIGQIVGEPLETFRLARGREAEQRVQELLETVGLSKRFIKRYP
                     HEFSGGQRQRIGIARALAVDPDFIVADEPISALDVSIQAQIMNLLEKLRREKNLTYLF
                     ISHDLRAIRHVSDRVAVMYLGKLVEIAEAKTIYKEPLMPYTKALISAVPVPDPQVEAT
                     RQRIMLEGDVPSPINPPSGCPFHTRCPYAIDVCKEVVPQLVEIKPAHFAACIRISPAQ
                     PHIENVAPGAAPGIE"
     gene            5403..>6194
                     /locus_tag="VFH96_03745"
     CDS             5403..>6194
                     /locus_tag="VFH96_03745"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HET6973110.1"
                     /translation="MVKKPPTSLNTQERREDTPPAWLEMQDSLAEASSLALLLVDGYQ
                     PPALAISNNNSICHTFQSSPEHVRLCDPYCGMAHSRAMEAGGTVEYKCHAGLSCFAKP
                     VEIGGRRKLAVIGGRAFVTSGDYQQLMERFRKGDLQDLAADEVFANIIFSDSQRLEEM
                     AERVDRAAGRLRHASSNGSGNVEAATTSPEPQPDLESEIQRLRRELEYRVRFTNSLQH
                     FLERISGSDPAKTYNSIVNNSKELLQSERASLMVFDETANQLILKA"
BASE COUNT         1379 a         1750 c         1691 g         1374 t
ORIGIN      
        1 caaatacacc gtgcggctct acctgacgca acaggcgccg ttcttcttag gtttacttgc
       61 acaccaggtc ttccgtttgg tgccgcgcaa ggcggtcgag cagtggggcg aacaatggac
      121 gcagcccgag cacatcgtca cttgtggtcc atttaaacta aaagaatggg tgccttacga
      181 caggctcgtg ctcgaacgcg atccgatgta ttgggacgca aagacagtga agctcgacga
      241 gctacaattc tttcccattc tcgacaacgc caccgtaatg aatctctaca aggtgggcga
      301 gcttgacgcg gtctacaacc acgcagtgcc gaccccgtgg ctgcacattc tcaaacagaa
      361 gaaagactac atggaccggg cggaggctgc gaatgattac ctgatcatga acaccactaa
      421 acctcctttg gataatccca ggattagaaa ggcgttcaat ctcgcaatcg ataaggaggc
      481 ctatgcgcgg tggcgccgga tcgtgaaacc gctaaccgca ttcacccctg aggggacgtt
      541 tgccggttac ccacagccca aaggtccggc cttcaatcca gcggaagcgc gtagactact
      601 cggcgaagtg ggttatccgg tagtgcaaaa ctcggacggc agtttttcgt gtccgaagtt
      661 tccggtggac caggtcgaat ttatttacaa cagccaggcc gcaaacaaat ccgtcgccga
      721 atggatgcag gcgcagtgga aacagaatct cggtatcact gttggactgc gaaacatgga
      781 ctggaagact tttctcgagg cgcgcggaaa gctcgagtac aagggattcg cgcgcggtgc
      841 gtggggcgcg gattatatgg acccgttcac gttcctgagc atcttctact cggcagaaga
      901 gagcggcacg ggctggctcg atccgaaata cgttgcgatg ctggatgaag ccaatcgcac
      961 tctcgatcac aaaaagcgtt atgagctgct tgccaaagca gaagcttaca tgctcgacaa
     1021 ccagccgatc attccgatcg acacaccatc ggtgaactgg ctgaaaaaac cgtacgtgaa
     1081 aggcatgtat ccgaatccag cgtctttgtt cgcgtggaaa tatgtttaca tcgagcgcga
     1141 ccgggcgaag tgggattacg gcacgccgag tttgaccgcc gagtagcgcg gtgaacgcgg
     1201 tctgtgatta acgaatgttt cggttcattg tcagacgact cctggtaacg attccgacga
     1261 tcctcgtcgt gatctcgatc acctgggtct tgatcaaact cgcccccgga aatttctatt
     1321 cgggtgagaa accactgcct cccgctgtcg agaaaatcat ccgcgaaaaa tacggcctcg
     1381 ataaaccctg gtatcaacag tacggaaaga cgatctgggg tatcgttcgc cacctcgatt
     1441 tcggtacgtc gctgagatac gaaggtcagt cagtaaattc aatcatcaaa cgctcgttgc
     1501 ccgtgtcagc cacggtcggc attcttgcct acctgcttgc gctcatcgtg ggcgtcacta
     1561 tcggatgtat tgcagcgctg aaacaaaact cgaagtggga ctacggctcg atggcgctcg
     1621 cgatgctcgg tatctcagtg ccgaactttg tgcttggtcc aatcctggtc ctgatctttt
     1681 cgcttggact ttactggctg ccaccgtctc gctggggcgg ctttccgagt cgaaacctgg
     1741 tcttgccggt actgactctg tcagcgcttt acatggctta catcgcgaga ctcacgcgcg
     1801 ccggaatgct cgaagtgctg cggtcagatt acatccgcac cgcgcgagca aagggtttga
     1861 gcgagcgtga tgttgtaatt cgtcacgcgc tgcgcggtgg tctgatgccg gtgatttcgt
     1921 acaccgggcc ggccctcgcg tttctgttaa ccggaactgt cgtggtcgag agaatctttg
     1981 cgttgccggg cttgggaaac tacttcatcc aggcgtcact gagtcgcgac gagccattga
     2041 tcatcggtat cgtcgcgttc atcgccataa cactcttgtt aatgaatctg ctggttgata
     2101 tcgcgtacgc gtatctcgat ccgcgcgtgc gctaccagta agtttgcgaa tggctaccga
     2161 aaagaaacca gaaccagacg atctgattca catcgggccg gagatggctg tggctgaagc
     2221 cgacctcatg cccggtgaag tgggccggtt tgtgcacggc acgtcgctgt ggcgcgatgc
     2281 ctggcgtcgt ctgctcaaaa acaagctcgc ggtttttggt ctggtcgtcg tggtcgtggt
     2341 tacgatctct tcgatcattg gaccgacagt gatccagcgc gtcttcggtt tcacgcctga
     2401 ttacattccg tcgaacgatc cgagactgac gcaatcgttt ccgcctttca ccggtccgaa
     2461 tggtgagttt tcatgggcgc atccgatggg aactgataac gccggacgcg atcagttcgc
     2521 ccgtgtgtta cagggcgggc agatctccct ctttgtcgga attctagcaa cgctcatatc
     2581 gttgttgatc ggcgtcagtt atggagcgat cgctggttac gtcggagggc gcgtcgacaa
     2641 cgtgatgatg cgcattgtgg acgtgctcta ttcgctgccg tacgtcatcg tggtgatcgt
     2701 gttgctgtcg atgtttcgca gccagacgcc gcgcggccag ttgatactgc tctttgtcgc
     2761 gattggaact gtgtcgtggc tgacgatggc gcggatcgtt cgcgggcagg tgctttcgtt
     2821 gaagaaccag gaatttgtcc tcgcggcgcg tgcgacgggt gtttcgaatg cacgcatcat
     2881 ctttcgacat atagtgccga acacgctcgg tccggtgatc gtctacgcga cgctcgagat
     2941 tccgcagatc atgttgacgg aggcgtttct ctcgttcctc gggtttggtg tgcaggctcc
     3001 gctcgcgtcg tggggcagtc tggcggccga tggaattcag aacatcgccg tcttcccgtg
     3061 gcaactgatc tttccgggcg tgacgatggc cttgacgctc ttctcgctaa acttccttgg
     3121 tgacggcttg cgcgacgcgc tcgatccgca gatgcgaact atataagtcc tataggacca
     3181 ataggaccta tttaagacta tgacctccag tgctaacggc aatcttctcg aagtaaaaaa
     3241 tctccgcact tacttcgaaa ccgacgacgg aatcgtcaaa gccgtcgacg acatctcttt
     3301 ccacctcaag cggggtgaga cgctcggcat cgtgggcgag tcaggctcag ggaagtctgt
     3361 caccaacctc tcgatcattc gtctcgtccc aacgccgccg ggaaagatcg tctcgggtca
     3421 ggtcttcttt aacggtaacg atctgctgca cctctcaaac gatgccattc gcaaaatccg
     3481 gggccgccgc atcgcgatga tctttcagga tccgatgacc tcactcaacc cgttcatgaa
     3541 gatctccaaa caactgatgg agatgaccga gctgcacctg ggccacacga aagcacaggc
     3601 gcacgctcac gccatccgca tgttggaaac cgtcggcatt cccgacgcac gtgaacgggc
     3661 cgacagctac ccccacgagt tttccggcgg catgcgccag cgtgcgatga tcgcaatggc
     3721 gctctcgtgt gaacccgagc tgctgatcgc ggacgagcca accaccgcgc tcgatgtcac
     3781 gatccaggcg cagatcctcg agctgataaa gaaactcaaa cgcgaaaccg gcacgagcgt
     3841 gatcctgatc acacacgatc tcggcgtcgt agcgggcatg accgatcacg tgatcgtgat
     3901 gtacgccgga aagatcttcg agcaggcgcc aacgaaggaa ttgttttcta ttcccggcaa
     3961 tccttacacg aaaggactgc ttcgctccgt acccgatccg accgccgaac agggcaagct
     4021 ctatcaaata ccaggactgc cgcctgacgt cgcacacctg aaacccggtt gtccttttgc
     4081 acagcgctgc gactacacgc aggatatctg cacgcgtgag tttccgccct tcgtgcagct
     4141 caatgcagat cactattcgc tctgccattt cgcgcgcgaa gtctacagcg aatcgggcgg
     4201 tacaccagta tgaactcaac cctgctctcc atcagagatc taaaagtaca tttcgatctc
     4261 ggcggtggtg gaccactcga caagttgttc ggcacgaatc ccgagaaacg cgtcgtgaag
     4321 gccgttgacg gcgtctcact cgacatctac gaacaggaaa cactcgggct cgtcggcgaa
     4381 tcaggttgcg gcaaatcaac gctcggtcgc gccatactcc gtctcaccga accgactgaa
     4441 ggccaggtgt tctatcgcga tcgcgacctc gcacacttgt cgcaaagaca gttgcgcgaa
     4501 cagcgacgac atctacagat catctttcag gatccatacg cgtcactgaa tccacgcatg
     4561 acgatcggtc agatcgtcgg cgaaccactc gagacgtttc gtctcgcaag aggcagagaa
     4621 gcggaacaac gcgtgcagga gctgctcgaa acagtcggtc tcagcaaacg tttcatcaaa
     4681 cgatatccgc acgagttttc cggcggtcaa cgccagcgca tcggcatcgc ccgcgcgctc
     4741 gcagtcgatc ccgatttcat tgttgctgac gaaccgatct ccgcgctcga tgtttcgatc
     4801 caggcgcaga tcatgaacct gctcgagaag cttcgtcgcg aaaaaaatct tacgtatctt
     4861 ttcatctcac acgacctgcg cgcgatcagg cacgtctcgg atcgtgtggc ggtgatgtac
     4921 ctgggaaaac tcgtcgagat cgccgaggcg aagacgatct acaaagaacc tttgatgcct
     4981 tacacgaagg cactgatctc ggcggtgcct gttcccgatc ctcaggtcga agccacgagg
     5041 cagcgtataa tgctggaagg cgatgttcct tcgccgatca atcccccttc cggttgccct
     5101 tttcacacac gctgccccta cgcgatcgac gtatgcaagg aggtggttcc tcaattggtc
     5161 gaaatcaaac cggcacattt tgctgcttgt attagaatca gtccggcgca acctcatatc
     5221 gagaacgttg ctcctggagc cgcgcccggc atcgagtgag ccacaaaaaa ggcacaaaag
     5281 tcacaaaaag aagaacttcg ggagtgtttt agccctttaa gtggggaata ttttgtgcat
     5341 tttgtgcctt attgtggcta ttactgtttt agagtaaggg aagatccttg cccagagccc
     5401 cgatggttaa gaagcccccg actagcctga acactcagga acgccgcgaa gataccccgc
     5461 ccgcgtggct ggagatgcag gactcactgg cggaggcttc atcgctcgca ttgttgctgg
     5521 tcgatgggta tcaacctccg gctttggcta tctcaaacaa caactcgatc tgtcacacct
     5581 ttcagagctc gccggagcac gttcgactgt gtgacccgta ctgcggcatg gcgcactcgc
     5641 gagcgatgga agccggcggt acggtggagt acaaatgtca cgccgggctc tcgtgttttg
     5701 cgaagcctgt tgagataggt ggccggcgga aactcgcagt gattggcggg cgcgccttcg
     5761 tcacgagcgg ggactaccaa cagttgatgg aacgttttcg caaaggcgac ctacaggacc
     5821 tcgctgccga cgaagttttt gcaaacatca tcttttctga ttcgcaacgt ctcgaagaaa
     5881 tggctgagcg agtcgaccgt gctgccgggc gactgcgcca cgcatcatca aacggctctg
     5941 gaaatgtgga agctgcaact acatcacccg agcctcaacc ggatctcgag agtgagatac
     6001 aacgcctgcg acgagagctc gaatatcgcg tgcgtttcac caactcgcta cagcatttcc
     6061 tcgaacgaat aagcggcagc gatccagcga agacttacaa ctcgatcgtc aacaactcga
     6121 aagagctgct gcaatcggag cgcgcctcgc ttatggtgtt cgatgagact gccaaccagt
     6181 tgatcctgaa ggct
//
LOCUS       DASSFQ010000111         8993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1683537, whole genome shotgun sequence.
ACCESSION   DASSFQ010000111 DASSFQ010000000
VERSION     DASSFQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683537"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFH96_03750"
     CDS             complement(<1..476)
                     /locus_tag="VFH96_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973111.1"
                     /translation="MAKHRLLFVFVGVAVIAVASLPFVLSASCVTRPQTPGEQRALDS
                     LRSMTRNDVLPSEDVVAGIENQFPRSKAAALARILRARIKLKAGDAAGAATLLDSRLI
                     GEQTSLADYALWMRGGALEQAGKAAEARAIYQELMQSHPSSLRARDAALRVADLMT"
     gene            complement(598..2019)
                     /locus_tag="VFH96_03755"
     CDS             complement(598..2019)
                     /locus_tag="VFH96_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973112.1"
                     /translation="MVNGHLEDSLRREIESYLEGRLSAVKQEITTLQSLLNESLSTFL
                     ERQSDVQMEGSMVAAIQEHLHAAHERGVELAASESSRAKASSDMAIVKASIQEINEQK
                     SQADILKALVNRASSFAPRVAFFVIKGEQATGWRGRGFEGTVGDDAVQQISFSVNADT
                     VVGSAAKGLATWSGGAGSHSEDHMIMNRLGEDQPQRVVAVPLKARGRAVAVLYADSAG
                     LDADSISLEALETLVAVSGMAVELLSVSRTGAAPKKAVEEAPAPAPVEEAQPAYEPAS
                     EYSEAPASAPEPEPESTFSGAETIQGYQPEPEPMSAEPSSFIEPVAADEPAGVFEDPS
                     MPAEQPISMGEEPPAWTPAPDALAPAPPRRRYGQDAELPVEVHGEEERRLHNDARRFA
                     RLLISEIKLYNEQKVAEGRMEHDLYDRLREYIDRSREMYDKRVKAEVANRYDYFHGEL
                     VNTLAEGDVSKLGSNYPGATVNA"
     gene            2287..2652
                     /locus_tag="VFH96_03760"
     CDS             2287..2652
                     /locus_tag="VFH96_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973113.1"
                     /translation="MRPTILIAEDYDDNRELLRLLLAGANYNVREARNGQECLDLARE
                     NPPDLIMVDLSMPELDGWEVFKALKASSATAHIPCVAVTAHTDRDRARALQSGFSDFV
                     GKPFRTEELLLTVARLVAK"
     gene            complement(2656..3852)
                     /locus_tag="VFH96_03765"
     CDS             complement(2656..3852)
                     /locus_tag="VFH96_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6973114.1"
                     /translation="MPQQTSTPKPPETVYLLKPAHVFDGESAQLHDNWVVLVRGEKIE
                     AVGPANTVNAPAGTKTIDLPGLTLMPGLIEAHSHVLLHPYSETIWNDQVARESLSLRV
                     ARATNHLRNTLQAGFTTVRDLGTEGAGYADVGLKQAVEQGIIPGPRMIVTTRAIVATG
                     SYGPKGYASEWTVPQGAEEADGVDSLTRVVRDQIGHGADWIKIYADYRWGKSGAAPTF
                     SLDELKLVVETAKSASIPVAAHATTAEGMRRAALAGVETIEHGDGGTPEVFRLMKERG
                     VALCPTLAIASGANVERKRTAFRAALDAGVTIASGSDVGVFAHGDNARELEAMVKFDM
                     PLIDVLRSATSVNARVLHMSDKIGAVKQGLLADLIAVDGDPTKDVEALRKVRFVMKGG
                     VVYREK"
     gene            complement(4014..5033)
                     /locus_tag="VFH96_03770"
     CDS             complement(4014..5033)
                     /locus_tag="VFH96_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6973115.1"
                     /translation="MSTSAAQQPSPVLFFQTINAHQRTEALKAAIELQVFTAIGEGNT
                     TVAEIAKRCETSEKGMRVLCDYLTIMEMLTKQNDRYALTLDSSVFLDKRSPAYIGGAT
                     EFLCSPMLTEGFRHMTEAVRKGGTAIDGEGTIGPDHPIWVNFARGMAGMMGMPAQLMA
                     KLVDPKADQKLKILDIAAGHGLFGIAFATNNPQAEITALDWKAVLEVAKENAAKAGVA
                     DRYSTIEGSAFDVDFGSGYDLVLITNFLHHFDPPTCETLLRKVHAALADGGRAVTLEF
                     VPNDDRVTPPDAAGFSLVMLAGTPAGDAYTFAELERMCANAGFSRSTMHPLPPTFQQV
                     VISEK"
     gene            complement(5086..6942)
                     /locus_tag="VFH96_03775"
     CDS             complement(5086..6942)
                     /locus_tag="VFH96_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973116.1"
                     /translation="MKLRMPEMPSATLLAALDGYNLLPAIVFMPTRRRCDQAASEAAL
                     ARRDPNDTRREARRDFMRAFVDQHPEVRGHRHWDTIIRGGVASHHAGHIPAWKLVIEK
                     LMSAGLLDAIFATATVAAGVDFPARTVVLTGADARSASGWRPLSASELQQMTGRAGRR
                     GRDNVGFVVAAPGIHQDPERIAQLLNVPPDPLVSQFRATYTTLLNLLDAYGSFASVRE
                     IAERSFAYRDFSRQIVQLEKSRSESEQKIHDALRDSGCAISTSTVLGLERLLGARARL
                     QEAKPQTRAEVFYRWLNEVVKPGRVVGIGRSGRRLVIVTEKRDGTVRGFREDGSSATF
                     PQERIGRVYSPMYRLKEEDVERAFDEIRTRGRELVLTEPRLRDADAEETEALKIIDDS
                     IENLLPSSVDKQQCTDIVWQLHTTAEDYERASRRIETLREEVWQPFEQRAQVLAVFGY
                     LDYEQEKVTDRGRWLADLHIDRPLLVGEALENGLFNSLAPKELAGIMAALTADEDRDY
                     GELELDDVVVASLSRFEDIGFKVSAEEWNHGLEPAPELNFSAAGAAVRWAGGTDWSQI
                     VRETRAEEGDLFRMFSRTGEALLQIAGLHRSHPLAANMAANVAEMVLREPIR"
     gene            complement(6939..7790)
                     /locus_tag="VFH96_03780"
     CDS             complement(6939..7790)
                     /locus_tag="VFH96_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6973117.1"
                     /translation="MAVVRRKSGKHWDKRRSGGRHHPEKSPRKTDQGANERAVRVVPP
                     SRELKRLLEGIGTPSPAPFKPDPFQLEALAALEYEDVLVTAPTGSGKTWIAREEIRRL
                     LDSERRAWYTSPLKALTNSKYQEFIDEFGAERVGILTGDRKENSDAPLIVGTTEIYRN
                     QLFDSLRGGSEVSADLVVLDEAHYLGDEDRGHVWEEAIILTPPRIRLLLLSATVGNAH
                     EFALWLEEIRGVHCGVITGPAKRPVPLRSAVLLPDKRLLPLLNDSGNLNPEIAKLAAD
                     ERRSERR"
     gene            7909..>8993
                     /locus_tag="VFH96_03785"
     CDS             7909..>8993
                     /locus_tag="VFH96_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973118.1"
                     /translation="MSELFEKFEVNREPRWIVLTRLIGASVVLHLILMTLVVYVPALR
                     DGLNIGALIANTKFVEKPYEATQIGDDVQFVQLNEKFHYPEGYFALEAQIDGKMPAQL
                     VAASDPFAPKIISQASKEKYVDPEASPSPSPGASGSPAGSPTASSSPVSPASAVAQAS
                     PSASPLTPAEAQKQLEKSAEQNGLTLPDENEINKQVLKDFANYANDLKTQGKLDFDKP
                     FEVVIEAELDDKGKLKNPRFTKKAGDENLVELFGKLVAALNDSGFLIYLQPITKDNPG
                     AKVKITVKQGEKEVLASVESETTSSERAESLAKTLNTALYFGATSRAGHDEETLMKNT
                     NASPDGKRVVVNFSMPRQSVVDMIQKN"
BASE COUNT         1929 a         2565 c         2513 g         1986 t
ORIGIN      
        1 ttcgtcatca agtctgcgac tcgcaacgca gcatcacgag cacgcagcga agatggatgc
       61 gactgcatta actcctgata aatagcgcgt gcttccgccg cttttccggc ctgttcgagt
      121 gcgccgcccc gcatccacag agcgtaatcg gcgagcgatg tttgttcgcc gatcaagcgg
      181 ctgtccagca gtgtggctgc tccggcagcg tcgcctgctt ttagtttgat ccgagcccga
      241 agtatgcggg caagcgccgc cgctttgctg cgcggaaact gattctcgat accggcgacg
      301 acgtcttccg acgggaggac gtcgttgcgc gtcatgctgc gcaggctatc aagagctctt
      361 tgttctccgg gagtttgcgg ccgcgtgacg cagctcgcgc tgaggacgaa tggaagcgag
      421 gcaacagcaa ttacagctac gccaacgaaa acgaaaagta agcgatgttt ggccacagta
      481 ataccagatc ctgtcaatcc tgaataatca tgtcaatcct gtgaagccgc ctctcttttg
      541 acaggattta catgattatt caggattaac aggatctgtc gtccaaaaca aaatgctcta
      601 ggcgttaacc gttgcgccgg gataattcga gccgagtttg gaaacgtcgc cttcggcgag
      661 ggtgttaacc agttcgccgt ggaagtagtc gtaacgattc gctacctcag ctttgacgcg
      721 cttgtcatac atctcacgag aacgatcgat gtattcgcgg agacgatcgt aaagatcgtg
      781 ctccatgcgt ccttcagcaa ccttctgctc gttgtacaac ttgatctcgg aaatcaacaa
      841 ccgcgcaaac ctccgcgcgt cattgtgtaa acgccgctct tcttcgccgt ggacttccac
      901 aggcagttcc gcatcctgac cataacggcg gcgcggcggt gcaggcgcga gtgcgtcggg
      961 cgccggagtc caggcgggcg gctcttcgcc catcgaaata ggctgctctg ccggcatcga
     1021 aggatcttcg aaaacaccag ccggctcatc ggccgcaacc ggttcgatga acgacgacgg
     1081 ctcagccgac atcggttccg gctcgggttg atagccctga atcgtttcgg cgccggagaa
     1141 agtcgattcc ggctctggtt cgggagcgct tgcaggcgct tcgctgtatt cactggctgg
     1201 ttcgtacgcg ggctgtgctt cttcaaccgg cgctggagca ggtgcttctt caactgcttt
     1261 cttcggagcg gctccagtgc gtgaaaccga cagcagctca actgccattc ctgacacggc
     1321 gacgagtgtt tccaacgctt cgagacttat tgagtctgca tcgagaccag ccgagtcagc
     1381 gtaaagaact gcgaccgcac gaccacgggc tttgagtgga acagcaacga ctcgctgggg
     1441 ctggtcttcg ccaagccgat tcatgatcat gtgatcttca gaatgcgagc cagcgccacc
     1501 gctccaggta gcgagtcctt tggcagcgct gccgacaact gtgtctgcat tcactgaaaa
     1561 agagatttgc tgaactgcat cgtctcccac agttccttcg aaaccacgac cacgccaacc
     1621 cgttgcctgt tcgcctttga tgacgaagaa agcgactcga ggcgcgaaag acgacgctcg
     1681 attcacgagg gctttgagga tatcggcctg agatttctgc tcgtttatct cctgaatact
     1741 cgccttcacg atggccatgt cgctcgacgc cttcgcacgg gaagactcag aagccgccag
     1801 ttcgaccccg cgctcgtgag ccgcgtgcag atgttcctgg atagcagcga ccatgctgcc
     1861 ttccatttgc acatccgatt ggcgctcgag gaaagtcgaa agcgactcgt tgagcaggct
     1921 ttgcagagtg gttatctctt gctttaccgc actaaggcga ccttcgagat aactctcgat
     1981 ctcacgccgc aaactatctt ccaggtgtcc gttgaccaca gctcgctcct ccgtgtcgtg
     2041 gtgtgtttgt tttgacgaac ctcaaggtct tcaggtaggg ataataaccc aaggttgatt
     2101 atcagcaatg gagagtcccg caggggccgc aagaaactcc gagtgattat gaacggctgg
     2161 tattcagcta gtcaagaaat cgtttttgtt gaattggcgt gcggggtgct aagagtggat
     2221 tcttttcgcc aatccgcctc atttggtaat ctaacggatc gggccgatag tcgtccacta
     2281 taaaatatgc gacctaccat acttatcgcg gaggactacg acgataaccg cgaactcctg
     2341 cggctgcttt tggcgggagc aaattacaac gttcgcgagg ccagaaatgg gcaggaatgt
     2401 ctcgatctgg ctcgtgagaa ccctccagat ctcatcatgg tcgacctttc catgccggaa
     2461 ttggacggct gggaagtgtt taaggcactg aaagccagtt cggctacggc gcacattcct
     2521 tgtgtcgcgg taactgccca taccgaccgg gaccgggccc gggctttgca atccggattc
     2581 agtgattttg tcggaaagcc gtttcgaacc gaagaactgc tgctgacagt ggcgcgtctg
     2641 gtcgcaaaat agaatctact tctcgcggta aaccacgccg cctttcatca caaatctcac
     2701 ctttcgcagc gcttcaacat ccttcgttgg gtcgccgtcg acggcaatca ggtcggcgag
     2761 caggccttgt ttcaccgctc caatcttgtc agacatgtgt agcacgcgcg cattcactga
     2821 agtcgccgat cgcaacacat cgatcagcgg catatcaaac ttcaccatcg cttccaactc
     2881 acgcgcgttg tcgccgtgag caaatacgcc gacgtcgctg ccactcgcga tcgtcacgcc
     2941 ggcatcgaga gctgctcgaa atgctgtccg cttcctttcg acgttagcgc cggaagcgat
     3001 ggcgagggtt ggacaaagcg cgacgccacg ctctttcatc aagcgaaaaa cttccggtgt
     3061 gccgccgtcg ccgtgctcga ttgtttcgac tccggcgagt gccgcgcggc gcattccttc
     3121 ggcagttgtc gcgtgcgcag ctacaggaat gctcgcgctc ttcgctgttt cgactacaag
     3181 cttgagctcg tcgagcgaaa aggtcggcgc ggcgccggat tttccccagc gatagtcggc
     3241 atagatcttg atccagtcag cgccgtgtcc gatttgatcg cgcaccacgc gagtaaggct
     3301 gtcgacgccg tccgcttctt ctgcgccctg tggaacagtc cattccgatg cgtagccttt
     3361 tggtccgtag ctgccggtgg cgacgatcgc tcgtgtagtg acgatcattc gcgggccggg
     3421 aatgatgcct tgttcaactg cctgtttgag tccgacgtcg gcgtaacccg cgccttcagt
     3481 tccgagatcg cgaacggtgg tgaatcccgc ctgaagcgtg ttccgcaagt ggtttgtggc
     3541 gcgggcgaca cgcaggctca atgattcgcg cgcgacctga tcgttccaaa tggtttcgga
     3601 gtaaggatga aggagcacgt gtgaatgggc ttcgatcaag ccgggcatca acgtcaggcc
     3661 cggcaggtcg atcgttttcg tgcccgctgg tgcgttgacg gtgtttgctg gtccaacggc
     3721 ttcgatcttc tcgccgcgaa cgagcactac ccagttgtcg tgcagttgtg ctgattcacc
     3781 gtcgaaaacg tgagccggtt tgagcaggta aacggtttcc ggcggcttcg gcgtcgatgt
     3841 ttgttgaggc atgaagaacg agagacagag aaacaataaa aggaagctac gcaacacagc
     3901 gcgaaccttt cgtcaggtgg aatcggacgt ctccttggag tgcggtgcct tggcaccgct
     3961 ttggtaaaaa ataccaaagc gccaacaagt tggcgcactc caaggaggtg caattacttc
     4021 tcggaaatga cgacctgttg aaacgtgggc gggagcgggt gcatcgtgct gcgcgagaat
     4081 cccgcattgg cgcacatgcg ttccaactca gcgaacgtgt acgcatcgcc agctggcgtg
     4141 ccggccaaca tcacgaggct aaagcccgct gcatccggcg gtgtcactcg atcatcattg
     4201 ggcacaaact ccaacgtcac tgcccgtccg ccatccgcga gtgccgcatg aaccttccgc
     4261 aacaacgttt cgcatgtcgg cggatcaaag tgatgcagga aatttgttat caggaccaga
     4321 tcgtaaccac tgccgaagtc gacatcgaaa gcgcttcctt caatcgtgct atagcgatcc
     4381 gcaacgcctg ccttcgccgc attctccttc gcgacttcca acacggcttt ccagtcgagc
     4441 gcggtaatct ccgcttgcgg attgtttgtc gcgaaagcaa tcccgaataa accgtgaccc
     4501 gcggcgatat ccagaatttt tagtttttga tctgcttttg ggtcgacgag ctttgccatc
     4561 aactgtgccg gcatccccat catcccggcc ataccgcgcg cgaagttcac ccagattggg
     4621 tgatccggac cgatcgtgcc ttcgccgtca atggcagtgc cgcctttacg cacagcttcg
     4681 gtcatgtgcc taaacccttc ggtcagcatc ggcgagcaaa gaaactcggt cgcgccgccg
     4741 atataggcag gcgagcgctt atcaagaaac accgaagaat ccagtgtcag cgcgtagcga
     4801 tcattctgct tggtcagcat ttccatgatg gtgaggtagt cgcagagtac gcgcattcct
     4861 ttttccgacg tctcacatcg cttagcaatt tcggcgacgg tggtgttgcc ctcgccgatc
     4921 gcggtgaaca cttggagctc gatggcggct ttgagagcct cagtgcgctg gtgcgcgttg
     4981 atggtttgaa agaataagac tggtgacggt tgctgggctg cggaagtaga cataagaatt
     5041 cctttcaggt ctgaataaat aatagaagta atctgtggac tgatgttacc gaatcggctc
     5101 acgtaaaacc atctcggcca cgttcgctgc catgttcgcg gccagcggat gactgcgatg
     5161 taaccccgca atctgcaaca gcgcttcgcc tgtacgcgag aacatgcgaa acaggtcgcc
     5221 ttcctcggca cgtgtttctc tgacgatttg cgaccagtca gttccgccgg cccaacggac
     5281 ggcggcgccg gcggcggaaa agttcaactc cggcgcgggc tctaagccgt gattccactc
     5341 ctcagcggag actttaaagc cgatgtcttc aaagcgagag agcgacgcga cgacgacgtc
     5401 gtccaattcg agctcgccat aatcacgatc ttcgtcggca gttaacgcgg ccatgattcc
     5461 ggcgagctct ttcggcgcca gtgaattgaa gagtccgttt tcgagggcct cgccgaccag
     5521 caacggtcga tcgatatgca gatcggccaa ccagcgcccg cggtccgtaa ctttttcctg
     5581 ctcgtaatcg agataaccga agacagccag cacttgtgcg cgctgttcga acggctgcca
     5641 cacttcttca cgcagtgttt cgatccgacg cgaagctcgt tcgtagtctt ctgctgtcgt
     5701 gtgtaactgc cacacgatgt cggtgcactg ttgtttgtcg acgcttgaag gcagcaggtt
     5761 ctcgatcgag tcgtcgatga tcttcagcgc ttcggtttct tcggcgtcgg cgtcgcgcag
     5821 tcggggttcc gttaagacga gttcgcgacc acgggtgcga atctcgtcaa aagcgcgttc
     5881 gacgtcttcc tctttcaatc gatacatcgg cgaataaacg cggccgatac gctcttgcgg
     5941 aaacgtcgcg ctgctgccgt cttcacgaaa gccacgcacg gttccgtctc gcttctccgt
     6001 cacaatcacg agccgtcgac ccgaacgacc gatgccgaca acgcgtcccg gcttcaccac
     6061 ttcgtttagc caacggtaaa agacttccgc gcgcgtctgt ggttttgctt cctgcaaacg
     6121 agcgcgggcg ccgaggagtc gttcgagacc gaggacagtt gacgtagaaa tggcgcagcc
     6181 tgaatcacgc aacgcgtcgt gaatcttctg ctcgctttcg ctgcgacttt tttcaagctg
     6241 cacgatctgg cgcgagaagt cgcggtaggc aaaactgcgc tccgcaatct cacgaacgga
     6301 agcaaaacta ccgtacgcgt cgagcaggtt gagcagagtc gtgtaagtcg cgcgaaactg
     6361 gctgacgagc ggatcgggcg gcacattcaa gagctgagcg atgcgttctg gatcctggtg
     6421 tatgccaggt gctgcgacga cgaaaccgac gttgtcgcgc ccgcgacgtc cggcgcgacc
     6481 agtcatctgc tgcagttcag aagcggataa tggacgccaa ccgctggcgc ttcgtgcgtc
     6541 agcaccggtc aagacgacgg tacgggcggg aaagtcgacg cccgcggcga ctgttgcggt
     6601 ggcaaagatc gcgtcgagca aaccagcgct cattagtttc tcgatcacca gtttccacgc
     6661 cggaatgtgt ccggcgtgat gcgacgcaac accgccacga atgatcgtgt cccagtgtcg
     6721 atgaccgcga acttccggat gctggtccac aaacgcgcgc atgaagtcgc ggcgtgcttc
     6781 gcgacgagtg tcgttcggat cgcgacgggc gagtgccgct tcggacgcag cctgatcgca
     6841 gcgacgacgc gtcggcatga acacgattgc gggaagtaag ttgtaaccat ctagtgctgc
     6901 cagcaaggtc gccgacggca tctccggcat gcgcagtttc atcgtctttc cgatctgcgt
     6961 tcatccgcgg ctaacttcgc aatctcggga ttcagatttc ccgagtcatt caacaacggc
     7021 aacaagcgct tgtcaggcag taacactgcc gaacgcaacg gcactggacg cttcgctggt
     7081 ccagtgatga cgccacaatg cacaccgcga atttcttcca gccacaatgc aaactcgtgc
     7141 gcgttgccga cagtcgccga caacagcaac aaccgaatcc gaggcggcgt caggatgatc
     7201 gcttcttccc aaacgtgccc acggtcttcg tcgccgagat agtgcgcttc atcaagtacg
     7261 acgagatccg cgctgacttc actaccaccg cgtagcgagt cgaaaagttg attgcgataa
     7321 atctcagtcg tgccgacgat cagcggcgcg tccgaattct ctttccggtc gcccgtcaaa
     7381 atcccgactc gctcggctcc aaattcgtca atgaactcct gatacttgga attcgtgagc
     7441 gcctttaacg gcgacgtgta ccaggcgcga cgttccgaat cgagcagcct gcgaatttcc
     7501 tcgcgtgcaa tccacgtctt gccgctgccc gtcggagccg tgacgagcac gtcttcgtac
     7561 tccaacgcag cgagcgcttc gagttgaaat gggtcaggtt tgaacggtgc cgggctgggc
     7621 gttccaattc cttccagcag gcgttttagc tcacgcgaag gtggaacaac gcgaaccgcg
     7681 cgttcgttag ccccttggtc agtcttgcgg ggagattttt cagggtgatg ccggccgcct
     7741 gaccgccgtt tgtcccaatg ttttccgctt tttcggcgca cgactgccat tctaactttg
     7801 ctcgttgtgg cctgcgaagt gcttaggcga accgggagag ttacaatctg tctcgcaacg
     7861 tgtggcgggc gatgggcgtc taaagccagg atctagcgaa attgcaggat gtcagagctg
     7921 ttcgaaaagt ttgaagttaa ccgcgagccc cgatggatag tcttaacgcg gctcatcggc
     7981 gcctcggtag tcctgcacct cattctcatg actctggtgg tttacgttcc tgccctgcgt
     8041 gacgggttaa atatcggcgc tctaattgcc aacaccaagt ttgtagaaaa accgtacgaa
     8101 gcgacgcaga tcggcgacga tgtgcaattc gttcaactca atgagaagtt tcattacccg
     8161 gaaggctact ttgcgctcga agctcagatt gacggcaaaa tgccggcgca attagttgct
     8221 gccagtgatc ctttcgcgcc gaaaataatc tcgcaggcaa gcaaagaaaa gtatgtcgat
     8281 ccggaagcgt ctccgtcgcc gagccctggg gcgagcggat ctccagcggg gagtcccacg
     8341 gcttcatcgt cgccagtgtc gccagcgtct gctgttgctc aggcaagtcc ttcagcttca
     8401 ccgctaactc cggcagaagc acagaaacag ctcgaaaaaa gtgccgaaca aaacggtttg
     8461 actttgccgg atgagaacga gatcaacaag caggtgctga aagacttcgc caactacgcg
     8521 aacgatctga aaactcaggg caagctggat ttcgataagc ctttcgaagt ggttatcgag
     8581 gctgagttgg atgacaaggg gaaactaaag aacccgcgct ttactaaaaa ggcaggcgat
     8641 gaaaatctgg tcgagctttt tggcaaactg gttgccgcgc tgaacgacag tggtttcttg
     8701 atctatcttc agccgatcac aaaagacaat ccgggcgcga aggtgaagat caccgtgaag
     8761 caaggtgaga aggaagtgct ggcttcagtt gaatcggaaa caacgtcgtc ggaacgcgct
     8821 gagtcgcttg ctaaaacact gaacacggcg ctgtactttg gcgcgacgtc gcgagccgga
     8881 cacgatgaag aaaccttgat gaagaatacg aatgcgtctc ccgacggcaa gcgcgtggtc
     8941 gtcaactttt ccatgccgcg ccaaagcgtc gtggacatga ttcagaaaaa tat
//
LOCUS       DASSFQ010000112         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1533755, whole genome shotgun sequence.
ACCESSION   DASSFQ010000112 DASSFQ010000000
VERSION     DASSFQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533755"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VFH96_03790"
     CDS             complement(<1..1533)
                     /locus_tag="VFH96_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973119.1"
                     /translation="MQNLITDIRFGFRSLLKRPGATAIALVTLALGIGVNTAIFSAVD
                     SILLRPLPFKDPERIVSVWEQTPSLGIQQNQAAPANFFDLRNQNQSFEALGAYGPLDI
                     NLTGAGDPERLDGQLVSANVFSILGVAPALGRTFLPNEDQIGQEHVVVLSDALWQRRF
                     NRDPSIINRNIALNGETFTVVGVMPRGFFFPQRETELWVPWAMEADQASGRADHYLGI
                     VARLKADSSIERANADLAGIGQRLAAEYPRTNEGLGFTARSLHRDYVGDLRLPMLILF
                     GAVGLVLLIACANVANLLLAQATTRRKEIAIRIALGARRWTIVRQMLVESLLLACGGG
                     LLGVLGAFWGVEALTKFLPESLSKLQGVNIDARVLLFTLGVSVFTAIVFGGLPALLAS
                     RTTPGSTLSDVARDMAGGTSGRHVRRLLVVSEVALAVVLLVSAGLLIRSFQLLRQVKP
                     GFNPENALTMRMVLPFPKYAKQEARSAFYDKVLRQVKETPGVEAAGMITFMPLSFSGM
                     NFS"
     gene            complement(1548..>2591)
                     /locus_tag="VFH96_03795"
     CDS             complement(1548..>2591)
                     /locus_tag="VFH96_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6973120.1"
                     /translation="EPSLLGYKDDKLTDLYLRAFSRLEAVPGVQSVTFSRSPLLAQSS
                     WTSSMYLPGVMGANGQPLDSDAKVHSVRENFFNTMEIPLLMGRGLTEQDDARAPRVAV
                     VNQAFVKAHFPNENPIGKRFGFESAKASEIEIVGVARDAKYTSQRDEIQPTVYQSWRQ
                     VLGRMTFATFEVRTAGDPAATIAGVREAMREVDSNLPLSNVRTQLQQADETLAMERMF
                     AKLLTLFGVIAQQLAAIGLYGIMAYVVTQRTHEIGIRMALGADRRNVLMMVVRQGMIL
                     TVVGIAIGLAAAYVLTKYLESLTSMLFGVEARDPWTFAAIGILLAVVALIACLVPARR
                     ATKVDPLRALRYE"
BASE COUNT          593 a          817 c          675 g          506 t
ORIGIN      
        1 actgaagttc ataccactaa acgagagcgg cataaacgtg atcatgcccg cggcttccac
       61 accgggtgtt tctttgacct gccgcaacac cttatcgtaa aaagcactgc gcgcttcctg
      121 ctttgcatac ttcggaaacg gcagaaccat gcgcatcgtg agtgcattct caggattgaa
      181 cccgggctta acttgccgca ataactggaa gctgcgaatc aacagtcccg cactgacgag
      241 caacacgacc gcaagtgcga cttcagagac gacgagcagt cgtcgaacat gacggcccga
      301 agttccgcct gccatatctc gcgccacatc actcaacgtg gagcccggcg tcgtgcgtga
      361 agcgagcaac gcgggcagac cgccaaagac gattgcagta aaaacactca cgccgagcgt
      421 gaacagcaac actcgtgcat cgatgttgac gccttgtagt ttcgaaagac tctcgggaag
      481 gaatttcgtg agcgcttcaa cgccccagaa ggcgccgagc acgcccagca acccaccgcc
      541 gcacgcgagt aataaactct cgacgagcat ctgtcgcaca atggtccaac gtcgcgcgcc
      601 gagtgcgata cgaatcgcga tctctttgcg tctcgttgtc gcctgtgcca aaagcaggtt
      661 cgcgacgttc gcacacgcaa tcagcagcac gagcccaacc gcgccaaaca aaatcagcat
      721 cggcaaacgc aggtcgccga cgtaatcacg gtgtaacgag cgtgctgtaa aaccgagccc
      781 ttcgtttgta cgcgggtact ctgcggctaa ccgctgcccg atccctgcga gatctgcatt
      841 cgcacgttca atgctcgaat ccgccttcaa acgagcgacg attcccaggt agtgatcggc
      901 acgtccactc gcctggtccg cctccatggc ccacggcacc cacaattcag tctcacgctg
      961 tggaaagaag aagccgcgcg gcatgacgcc cacgaccgta aacgtttccc cgttgagcgc
     1021 gatgttgcga ttgatgattg acggatcgcg attgaaacgt cgctgccaga gcgcgtcgct
     1081 tagcacaacc acatgctcct ggccgatctg atcttcattc gggagaaacg tacgccccag
     1141 tgcaggcgcg acgcccaaaa tcgaaaacac gttcgcagaa acgagttgac catcgagccg
     1201 ctcgggatca ccagcgccgg tgaggttgat atccagtggc ccatacgcgc ccagcgcttc
     1261 gaacgactgg ttctgattcc gcagatcgaa aaagttcgcg ggcgccgcct ggttttgctg
     1321 tatgccgagt gacggcgtct gctcccaaac agatacgatt cgttccggat ctttaaatgg
     1381 cagcggccgc aggagaatcg aatcaaccgc gctaaagatc gccgtattaa ccccgatccc
     1441 caatgcgagc gtcacgagcg cgatcgcagt cgcccccggc cgcttcaata aactgcggaa
     1501 cccaaagcga atatcagtta tcaggttttg cataggtcct gttggtccta ttcgtaacga
     1561 agagcgcgca gcggatccac cttcgtggcc cgtctcgccg gcaccaaaca cgcgatcaac
     1621 gcgaccacgg ccaataggat cccgatcgcc gcaaaggtcc acggatcgcg cgcctcaaca
     1681 ccaaacagca tgctcgtcaa actctcgaga tacttcgtca acacgtaagc agcagctaat
     1741 ccgatcgcga taccaacaac cgtcagtatc attccctgtc gcacgaccat catcagcacg
     1801 ttgcgtcgat cggcgcctag cgccatgcga atgccgatct cgtgcgtgcg ctgcgtcacg
     1861 acatacgcca tgatcccgta caacccgatc gccgcgagct gttgcgcgat gacaccgaag
     1921 agcgtcagca acttggcaaa catccgctcc atcgcgagtg tttcgtcagc ttgttgcagt
     1981 tgtgtgcgga cgttgctgag cggcaggttg ctatcgacct cgcgcatcgc ttctcgtacg
     2041 cccgcgatag tcgctgccgg atcgcctgcc gtgcgcactt cgaacgtggc aaaagtcatc
     2101 cgtcccagca cttgtcgcca tgattgatag accgtcggct ggatctcgtc gcgctggctg
     2161 gtgtacttcg cgtcacgcgc cacaccaact atctcgatct cgctcgcttt ggccgactca
     2221 aagccaaatc gttttccgat tgggttttca ttcggaaagt gagctttcac aaatgcctgg
     2281 ttgacgacgg caacccgtgg cgcgcgcgcg tcgtcctgtt cagtaagacc gcggcccatg
     2341 aggaggggaa tctccatcgt gttgaaaaag ttctcacgca ccgaatgcac cttcgcgtcg
     2401 ctgtcgagcg gctggccgtt ggcccccatc acgcccggta gatacatcga actggtccat
     2461 gagctctgag ccaacaatgg actgcgtgaa aaagtaaccg actgcacgcc gggtactgct
     2521 tcaagacgac tgaacgctcg tagatacaga tcggtaagct tgtcgtcctt gtatcccagc
     2581 aagctcggct c
//
LOCUS       DASSFQ010000113         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_941078, whole genome shotgun sequence.
ACCESSION   DASSFQ010000113 DASSFQ010000000
VERSION     DASSFQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941078"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFH96_03800"
     CDS             <1..732
                     /locus_tag="VFH96_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973121.1"
                     /translation="IPAAFVADPFMLHRDHTWYMFFEVMHATTRLGEIGLATSNDALT
                     WTYDRIVLKEPFHLSYPHVFEWQNHTYMIPEMLRADAVCLYKALDFPYNWTQVAELLK
                     GKHADPSLLRFNDRWWLFTCSPPYQHDTLKLYCADELTGPWTEHPKSPLIQNDKSRAR
                     PAGRILKLGSRLFRFAQDCALRYGSSVRAFEITSLTKDNYAEVELRIPILKASGNGWN
                     AKGMHHIDAHQQANGRWLACVDGIQ"
     gene            741..1793
                     /locus_tag="VFH96_03805"
     CDS             741..1793
                     /locus_tag="VFH96_03805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973122.1"
                     /translation="MRILLASPHRYPAFGSTGSGLHPRTYPSGSGFWIHDLLMKGLTE
                     LGHEVFYFLADGAAMPLPPGAKLVSKPVWDCDILHTISDQFPNLTREWEASGRPWVAT
                     CHIDMRTIGVSQRATTENWIFVSRTLARLHGRTRFVLNGIDPDACMYSEQKRDYFLFM
                     SSMDWEMKKGLDIALSLAARHGIKLVVAGTGNNYKRIMRITSLCREIGAEYVGDVRGS
                     RKAELLASAKAFLFPTRLDEAFGLGMVEALMSGTPVICSDKGACTEIMTRDVGFICRY
                     ESDYLNAIRRIGLISPQACCEKAMRDFHYLRMAQDYVAEYEKEIRANRLRDLSNPRSL
                     DRDCRDEVHHHERARG"
     gene            complement(1737..2138)
                     /locus_tag="VFH96_03810"
     CDS             complement(1737..2138)
                     /locus_tag="VFH96_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET6973123.1"
                     /translation="MVNKTRIKTDGGPPALGTYSQAIRTGDLVFVTAQTGRDPDTGKL
                     EEGLEAQTRRMFSNLDAILNAAGCSAADVVKVTLLLADIKDFKAVDVIYAEWLPGLGI
                     TPLPARTAFAAATLPAGALVMMDLVAAIPIE"
     gene            complement(2131..>2598)
                     /locus_tag="VFH96_03815"
     CDS             complement(2131..>2598)
                     /locus_tag="VFH96_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973124.1"
                     /translation="RDVTHFLQRLTDGDYEVETEREEFVRENISLKQHGNLPRERGST
                     KRPYEQSWQYAFEHFDLTKKILTDVPLEIRTEPMESDDFRQWIRAQNNLWIYASNITE
                     FHFFDLEFANPTNVVLLQIIFPGDVQLLDLAPLAGGPVKVDFHIPLKAGRING"
BASE COUNT          560 a          783 c          684 g          571 t
ORIGIN      
        1 atccccgctg ccttcgtcgc cgatccgttc atgctccacc gcgaccacac ctggtacatg
       61 ttcttcgaag tcatgcacgc tacgacacgc ctcggcgaga tcgggctcgc caccagcaac
      121 gatgcgctta cctggactta cgatcgcatc gtgctcaaag agccctttca cctctcgtac
      181 ccacatgtct tcgaatggca aaaccacact tacatgatcc ccgaaatgct cagagccgat
      241 gccgtttgtc tctataaagc tctcgatttc ccctacaact ggacccaagt cgcggaattg
      301 ctcaaaggca agcatgccga cccgtcgctc cttcgcttca acgatcgctg gtggctattc
      361 acctgttctc cgccttacca gcacgacacg ctgaaactct attgcgcgga tgaattaact
      421 gggccatgga ccgagcatcc aaagagtccg ctgatccaaa atgataagag ccgcgcgcga
      481 cccgccggac gcatcctgaa acttggcagt cgtctctttc gctttgccca ggactgcgca
      541 ctaagatacg gctcgagtgt gagagcattt gagatcacaa gtttgacgaa agataattac
      601 gccgaggtcg aacttcgtat cccgattctc aaagccagtg gtaacggctg gaacgccaaa
      661 ggaatgcacc acatcgacgc gcaccaacaa gctaacggcc gctggctcgc ctgcgtcgac
      721 ggcatccaat aaccaagaac atgcgcatac tgctcgcgtc cccacatcga tatccggcct
      781 tcggctcgac cggcagcggt ctgcacccgc gcacctatcc ttcgggctca ggcttctgga
      841 ttcacgactt gttaatgaag ggactgacgg agctcggtca cgaagttttc tatttcctcg
      901 ctgacggtgc cgccatgccc ttgccgccag gagcaaaact cgtctcaaaa ccggtctggg
      961 attgcgacat actgcacacg atctccgatc agtttccaaa cttaacgcgc gagtgggaag
     1021 cgagtggtcg cccgtgggtc gcgacctgtc acatcgacat gcgcactatc ggcgtgtcgc
     1081 aacgtgctac caccgagaat tggattttcg tctcacgcac tctcgcgcgt ctccacggcc
     1141 gcacgcgctt cgtgctcaat ggcatcgatc ccgatgcgtg catgtactcg gagcagaagc
     1201 gcgattactt tctgttcatg tcgtcgatgg attgggagat gaagaagggc ctcgacatcg
     1261 cgctctcact cgcggctcga cacggcatca agctcgtcgt ggcgggaacc ggaaacaact
     1321 acaaacgcat catgcgtatc acgtcgctct gtcgcgagat tggcgcggag tatgtcggtg
     1381 atgtgcgggg ctcgcgcaaa gcggagctgc tcgccagcgc gaaagcattt ctcttcccaa
     1441 cacgcctcga cgaagcattc ggactcggaa tggtcgaagc cttaatgtct ggtacacctg
     1501 tgatatgcag cgacaaaggc gcgtgcaccg agatcatgac tcgcgacgtg ggcttcatct
     1561 gccgctacga gtccgattat ttaaatgcga tccgccgcat cggactcatc tctcctcaag
     1621 cctgctgcga aaaagccatg cgcgactttc attacctgcg catggcccag gattacgttg
     1681 ccgaatacga gaaagaaatc cgcgcaaatc gtcttagaga tctctctaat ccgcgttcac
     1741 tcgatcggga ttgccgcgac gaggtccatc atcacgagcg cgcccgcggg tagtgtcgcc
     1801 gccgcaaatg cggtccgtgc cggcagtggc gtgataccca aacccggaag ccactcagcg
     1861 tagatcacat cgacggcttt gaagtctttg atatcggcca gcagcagtgt cactttaaca
     1921 acatcagccg ccgaacaacc cgccgcattg agtatcgcgt cgagattcga aaacatgcgg
     1981 cgcgtttgtg cttcgagtcc ttcttcgagc ttgcccgtgt ccggatcgcg tcccgtctgt
     2041 gccgtcacaa aaaccagatc gcccgtgcga atagcttgcg agtaagtgcc gagtgccggc
     2101 ggcccgccgt ctgtcttgat gcgtgtcttg ttaaccatta atacgacctg ctttcaatgg
     2161 aatatgaaaa tcgactttga ctgggccacc ggcgagtggc gcgagatcga gtagctgcac
     2221 gtcgccggga aagatgatct gcaacagcac tacgttcgtc gggttcgcaa actccagatc
     2281 gaagaaatga aactcggtga tgttcgaagc atagatccag aggttgttct gtgcgcggat
     2341 ccattggcga aagtcgtcac tctccatcgg ctcggtgcgg atctcgagcg gcacgtcagt
     2401 caggatcttc ttcgtcagat cgaagtgctc gaaagcgtat tgccagcttt gctcgtacgg
     2461 ccgcttcgtc gagccacgtt cgcgcggcag gtttccgtgc tgcttcaggc tgatgttttc
     2521 gcgcacgaac tcttcgcgtt ctgtttcgac ttcgtaatcg ccgtcagtga gtcgctggag
     2581 aaagtgtgtg acgtcgcg
//
LOCUS       DASSFQ010000114         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1684920, whole genome shotgun sequence.
ACCESSION   DASSFQ010000114 DASSFQ010000000
VERSION     DASSFQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684920"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFH96_03820"
     CDS             <1..454
                     /locus_tag="VFH96_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973125.1"
                     /translation="EQPATPASSPESRPEPRSEPRAEPQPERRPEPRVINNDRPTKTF
                     ETRSEPRSEPRSEPRSEPRESRPEPRRETPRSEAPAPRNDPPPRHDPPPRNDPPPRND
                     PPPRNDPQPRNDPPPRNDPPQRVESPRSESPKPVPDKPDRPMERPIDN"
     gene            complement(524..979)
                     /locus_tag="VFH96_03825"
     CDS             complement(524..979)
                     /locus_tag="VFH96_03825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6973126.1"
                     /translation="MTIRSAAPEDGPTLTTIALDAKRYWGYPEHWIKHWESDLTISPE
                     FIRDNHVYVHEEDGEIRGFYALCVAGDKAELEHMWVTPSAIGTGIGKELFLDAMERAA
                     KLNVNAVEISSDPNAAGFYKRMGATQIGEVDAPVDGEPRKLPRLEIKPS"
     gene            1003..1932
                     /locus_tag="VFH96_03830"
     CDS             1003..1932
                     /locus_tag="VFH96_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET6973127.1"
                     /translation="MTTEIIVFLERNTFNVPFRRPAFAHEWSEFGETAPSEVVERLAP
                     ATIAICNKLPLRAETLSQLPKLKLIAVAATGVDNVDLAYCRGHNIAVCNTRGYAVNSL
                     PEHALMLMLALRRNLIAYRNDVKAGRWHEAKQFCLLDHPIGDLRGATLGIVGFGTLGK
                     SMAQLGRAIGMEVLVAVRNDRAGFQEVLRRSDVLSLHCPLTEETKNLIGADELAQMKP
                     EAILINTARGGLIDDRALIEALKSGRLAGAGIDVLRNEPPRDGNPLLEVDLPNLIVTP
                     HNAWASRQAMQTLADQLVDNLEAFVRGSPQNLV"
     gene            complement(2007..2234)
                     /locus_tag="VFH96_03835"
     CDS             complement(2007..2234)
                     /locus_tag="VFH96_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973128.1"
                     /translation="MSENESAIGEGAATATEPEASTEEITYHAVEKDGLVTAIWEMQG
                     RKHLRTIDPRSRQGQDILRLYTTEHHDEAAV"
     gene            complement(2298..3383)
                     /locus_tag="VFH96_03840"
     CDS             complement(2298..3383)
                     /locus_tag="VFH96_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6973129.1"
                     /translation="MDADERYTGRGVTVAFLDSGFYAHKDLVEPENRILAYHNIFEPE
                     EDVAALQKSDVASWHGMMTSVVAAGNGHLSDGLYRGIAPEANVVLVKIGKTGRIPESN
                     IETGLRWVFENRDKYKIRIVNISAGGDFEQSYLKNALCQLVEEVAKAGLTVVCAVGNA
                     GMLPGHPVLPPASAPSCIAVGGLDDQNSLDRARRGMYRSSYGPTIDGLQKPEVIAPGI
                     WVAGPILPHTPTADQAGLYAQLDNAGDDELREIILAHASVDKDIYAARDLSLSLLRQL
                     ITIKLREGNVINEHYKFVDGTSFAAPIVSSIVACMLEANPKLTPQQVKRILIDSAERV
                     DGVEVDRQGWGVVVPRKAVEMALRCRG"
BASE COUNT          808 a         1081 c          970 g          766 t
ORIGIN      
        1 tgaacaacct gcgacgcccg cgtcgtctcc ggaatcacga ccggagccgc gatctgaacc
       61 aagagccgag ccgcaaccgg agcgacggcc agagccacgg gtgatcaaca acgatcgccc
      121 aaccaagacc ttcgaaacac gttcggaacc acgatccgag ccacgttcag agccacgttc
      181 agagccacgc gagtctcgac cagaaccacg ccgcgaaacg ccacgctctg aagcgccagc
      241 gccacgaaac gatccgccgc cgcgtcacga cccaccacca cgcaacgacc cgcctccgcg
      301 caacgatcca ccaccacgca acgatccgca gccgcgcaac gatccgccac cacgaaacga
      361 tccaccgcag cgtgtcgagt caccgcgaag tgagtcacca aagcctgttc cggacaagcc
      421 cgatcgtccg atggagcgcc cgatagacaa ttaatccaca agaggcacaa aaaggaacac
      481 ataagtcaca aaatcattta tgtgacttat gtgccttttg tggctaagaa ggcttgatct
      541 cgaggcgtgg taactttcgc ggctctccat cgacaggtgc atcgacttca ccgatctgcg
      601 tcgcgcccat gcgtttgtag aatcctgccg cgttcggatc cgaagagatc tcgaccgcat
      661 tcacattgag ctttgcggca cgctccatcg cgtcgagaaa caactctttc ccgatacccg
      721 tgccgatcgc cgacggcgtc acccacatgt gttccagctc ggctttatcg cctgcgacac
      781 aaagcgcgta gaagccacga atctcgccat cctcttcgtg aacgtagacg tgattgtcgc
      841 gaatgaactc gggtgagatc gtgaggtccg attcccagtg tttgatccag tgttcgggat
      901 aaccccaata acgcttagcg tcaagcgcga tggtagtcaa ggttggtccg tcttccggtg
      961 ctgcgctcct gatagtcatt tcagtgctta taataaccgg atatgacaac agaaatcatc
     1021 gtctttctcg aacgaaacac cttcaacgtt ccgtttcgaa gacctgcgtt tgcgcacgag
     1081 tggagcgagt ttggcgaaac agcgccgagc gaagttgtcg agcgcctcgc gcccgcgacg
     1141 atcgcgatct gcaacaagct tccacttcgt gcggaaacac tttcacagtt gccaaagcta
     1201 aaactgattg cagtcgctgc gacgggcgtc gataacgttg atcttgcgta ttgtcgcggt
     1261 cacaacatcg cggtctgtaa cacgcgcggc tacgcagtga attcattgcc cgagcacgcc
     1321 ctcatgttga tgctggcgct gcgtcgcaat ctcatcgcgt atcgcaacga cgtgaaagca
     1381 ggacgctggc atgaggcaaa acagttttgt ctactcgatc atccgatcgg agatctgcga
     1441 ggtgcgacac tcggaatcgt gggctttgga acactcggaa agtcgatggc ccaactcggg
     1501 cgtgcgattg gaatggaggt gcttgtcgcg gtgcgtaatg atagagccgg atttcaggaa
     1561 gttttgcgac gcagcgacgt cctctcgtta cactgcccgc ttacagaaga aaccaaaaac
     1621 ctgatcggcg cagatgagct cgcgcaaatg aaacccgagg caatcctcat caacaccgca
     1681 cgcggcgggc tgatcgacga tcgcgcgttg atcgaggcat tgaagagtgg acggcttgct
     1741 ggtgcgggta ttgacgtgtt gcgaaacgag ccaccgcgtg atggaaatcc gttactcgaa
     1801 gtcgatctgc caaacctgat cgtgacgcca cacaacgcat gggccagccg gcaagcaatg
     1861 cagacgcttg ccgaccagtt ggtcgacaac ctcgaagcgt tcgtccgcgg ctcgccccaa
     1921 aacttagttt gagccacaga tgaacgcgga aaaaacacgg atttaaatcc gtgcttaatc
     1981 cggtcttcaa tccgtgtcat ctgtggctaa actgctgctt cgtcgtgatg ctcggtggtg
     2041 taaagacgaa ggatgtcctg tccctggcgt gagcgtggat cgattgtgcg caggtgcttg
     2101 cgaccctgca tttcccaaat agccgtgact agtccatctt tctccacagc atgatacgta
     2161 atctcttcag tgctcgcttc aggctcggtt gctgtcgccg cgccctcgcc tattgcagat
     2221 tcattctcag acatacgaac tcaaacattc tccaatctct tcggtaggtt ttttacggtg
     2281 tcgcgaattg ttgcacatca cccacggcaa cgcaacgcca tctcaaccgc tttgcgcggc
     2341 acgacgacgc cccagccctg tcgatcgact tccactccgt caacgcgctc ggcactgtcg
     2401 atcaggatgc gcttcacttg ttgtggcgtg agttttggat ttgcctcgag catacaggcc
     2461 acgatcgacg agacgatcgg cgccgcgaaa gacgtgccgt caacaaactt gtagtgctcg
     2521 ttgatcacat ttccctcgcg caacttgatc gtgatcaact gccgcaatag cgacagtgac
     2581 agatcgcgcg ctgcgtagat gtctttgtcg acactcgcgt gcgcgaggat gatctcgcgt
     2641 agctcgtcgt caccggcgtt atcgagctgc gcgtagagtc ccgcctgatc ggcggtcggc
     2701 gtgtgcggca ggattggacc agcaacccag atcccgggcg cgatcacttc aggtttctgc
     2761 agtccgtcaa tggttggacc atacgacgag cgatacatgc cacggcgcgc gcgatcgagt
     2821 gagttctgat cgtcgagacc gccgactgca atgcacgacg gcgcactcgc cggcggcaac
     2881 accggatgtc ccggcagcat tccggcatta ccgacagcgc aaactaccgt cagtccagct
     2941 ttcgccactt cttcaacgag ctggcacaac gcgtttttca ggtaagactg ctcgaagtcg
     3001 ccgccggcag agatgttgac gatgcgaatt ttgtacttgt ctcgattttc aaacacccag
     3061 cgcagcccgg tttcgatgtt gctctccgga atgcgtccgg tcttgccgat cttgacgagc
     3121 acgacattcg cttccggcgc gatgccgcga tagagaccgt cagataaatg accgttaccc
     3181 gccgcaacga ccgacgtcat catgccgtgc cagctcgcga catcagactt ttgcagtgcc
     3241 gcgacatcct cttcgggctc aaagatgttg tgataggcga gaatgcgatt ttccggctcg
     3301 acgaggtctt tgtgtgcgta gaagccggag tcgagaaatg ccaccgtaac accgcggccc
     3361 gtgtaacgtt cgtcagcgtc catccgcacc gggactggta gcgcctgctc aattatttca
     3421 gttatattat ccagcgactc cactgttaca gattacacga gaattctaag tagcgttaaa
     3481 ggagagttgt aggatgagag ctaaaaatct atcgattctg ttcgccgcgt tgtttgtgat
     3541 tgcgggcgcc gttctggcta cgggcgcgcg cgccgaaggt gaagtgcccg cgccgcctgc
     3601 gattggcgcc accatcgaag acttt
//
LOCUS       DASSFQ010000115         6440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1237183, whole genome shotgun sequence.
ACCESSION   DASSFQ010000115 DASSFQ010000000
VERSION     DASSFQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237183"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFH96_03845"
     CDS             <1..227
                     /locus_tag="VFH96_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973130.1"
                     /translation="EINRYINNVQAVSTADIQKFAGTRLDAKTSNIIIVGNAKAFLPE
                     LQKKYANVEVIPITELDLDTALLKKKQPQI"
     gene            254..871
                     /locus_tag="VFH96_03850"
     CDS             254..871
                     /locus_tag="VFH96_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973131.1"
                     /translation="MRVIFALVVLSFVCASSHAQQEPIDGVDARSNPAEFAKGLGSSD
                     PLVRQKSAEALAKLAATDQRKLVEGYQLQEKNKEVHLALDWALYRMGKAEALYRVVDD
                     LDSGRQQQAVGYLSELESPDVLYPFLKRANNAPRINAGLLKALAHIGDAQTLELVKPF
                     RESHQPYVAEAAEIASDEIEKRLSEPAANATRARPRTVEKAPDRP"
     gene            880..2637
                     /locus_tag="VFH96_03855"
     CDS             880..2637
                     /locus_tag="VFH96_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET6973132.1"
                     /translation="MHASLRTFLDLLNRENEITTIKAEVDPYLELAEIHRRVIAEGGP
                     ALLFERVKGSRYPVVTNLFGTERRIDLAFGPKPEAFVRQMVHIAESILPPKFGELWQH
                     RSVALELAKLGTRNTSRSPVTQVVDRPARLDELPVLTTWQEDGGPFITLPLVYTENPI
                     TKKHNLGIYRVQVYDSHTTGLHWQIQKGGGFHYSEAERLNQPLPVTIFLGGPPALILA
                     AIAPLPEDVPELVLASLLAGQKLKMTRNPLGEGRHRLAAEAEFALVGHAPPHQRRPEG
                     PFGDHYGYYSLRHDYPVFNVEAVFHRRDAIYPATVVGKPRQEDFFIGDYLQKLLSPLF
                     PLVMPSVRDLWTYGETGFHSLAAAVVQERYGREALVSGFRILGEGQLSLTKFLILTDT
                     PQDLKDFPRLLEYVLERFRPETDLFVFSNVSMDTLDYTSGKVNEGSKAIMLGLGEGVR
                     KLPREFKGELPASVKRAEVFCGGCLVLEGGARAEDVANDPVFAEWPLIVLHDDARVAK
                     SSTDFLWATWTRFEPASDISARRTEVRRHHLVYTAPIVIDARTKPGFPDELIVREDIK
                     TLVDQRWKEYFSHGFKSGF"
     gene            complement(2645..3997)
                     /locus_tag="VFH96_03860"
     CDS             complement(2645..3997)
                     /locus_tag="VFH96_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973133.1"
                     /translation="MVRRPIPVTYRNRFQSGLTMLTGLFLLAVLFAVLLYAFTPETPA
                     ARSPILLAAFIAILLGSLLCILFVLRWLLRPYQQLVGEAQRASVSAPHKSQDEAEFVL
                     ETFQSVVAQLQEQRKELEKFSERIVASLPSGLIAFDGAGLSITINTPGRTLLEVNGAA
                     LGESYKQLFGAHEELAKMVGDCLQTGTVYRRTEIEAVSENGKLRRLGATVAPIDLPPE
                     RGPRGALCLLTDITEVTELREQVALKNNLESLGEMSAGLAHEFKNAIATLQGYAQLLQ
                     SMELNDKAQVAAASMLNEVRNLSGMVTAFLNFARPQPLHLEEVSLSDLIADCATELKV
                     VVDVDPSLTIRADERMLRLALLNLIRNGAEAAPDGLVTVSAAREDGNVVIQVRDTGPG
                     ISPADLQKIFIPFFTTKAKGHGIGLALTHRIITQHGGTLTATNSPDGGALFTIKLSHG
                     "
     gene            complement(3973..4842)
                     /locus_tag="VFH96_03865"
     CDS             complement(3973..4842)
                     /locus_tag="VFH96_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET6973134.1"
                     /translation="MIDLNLVPPVVGYVIAGVFGAIIGSFLNVVIHRVPNEESIVFPN
                     SRCPSCGAVIAFYDNIPVLSYLLLGAKCRKCKEHISFRYPAVELLTAALFVGVAWHDQ
                     LSAALPFDLVFASALLALVFIDAEHMILPNVITYPGIAFALVARVAIPLLTGTPHFDD
                     IPSLSHGALAGMPLWVISLAGAFIGALIGGGSLWLMGWTWEKLRGIEAMGLGDVKMMF
                     MVGAYLGWRLTILTIFVGVLSGSLIGILLMAKQGQRNMQMLLPFGVFLGLGAIAALLF
                     GAPLVEWYAGQFR"
     gene            complement(4839..5618)
                     /locus_tag="VFH96_03870"
     CDS             complement(4839..5618)
                     /locus_tag="VFH96_03870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6973135.1"
                     /translation="MKISATIITLNEESNIKAACESVAWADEILVVDSGSTDATREIA
                     AVCGARVITNPWPGFGAQKQFAVEQTQHEWIFSLDADERVSDELKRSIESLKQKPILA
                     DGYEIARRTYYQGRWIRGGGWYPDRQLRLFRKNKGQWKQRHIHESVTMDPGARIERLN
                     GDLLHYTSQNAAHHHRMIGERYAPLAARQMFEEGRRTSVIGVASAGPAAFIRSLILKG
                     GLRDGFAGFTIASFAAHHAFLKHLMLWEKQAADKTDKNGSR"
     gene            complement(5731..>6440)
                     /locus_tag="VFH96_03875"
     CDS             complement(5731..>6440)
                     /locus_tag="VFH96_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6973136.1"
                     /translation="TKPRIDFTGTVALTGLAGNNLATIVPPGATQPLIVGNPLTDPSA
                     FLLNEIQNIQRAQGLPVAVPPLAVVDANVVNPKLIGGYGQTLQNLFGGETRNVTLGVA
                     IQFPFKNKTAEANLAGARIQKEQLQATVRSQEQVIEVDVRNSAQAVESARQRVLSARE
                     ARTNAEMQLEGEQRLYQVGRSTTFLLFQRENALANARASELRAETDYNKALADLQRAT
                     STTLRANNVVVDTPLQP"
BASE COUNT         1427 a         1784 c         1845 g         1384 t
ORIGIN      
        1 atgagatcaa tcgctacatc aacaacgtgc aggcggtgag cactgcggac attcagaagt
       61 tcgcggggac ccggctggat gcgaagacga gcaatatcat catcgtcggg aatgcgaagg
      121 cctttctgcc tgaattgcag aagaagtatg cgaatgttga agtgataccg atcactgagc
      181 tggatctgga taccgcgctg ttgaagaaaa agcagccaca gatttaacgg atcgaaaatc
      241 gggttgaacg gatatgagag ttatttttgc tctggttgtg ttgtcattcg tttgtgcttc
      301 atcacacgcg caacaggagc cgatcgatgg cgtggacgcg cggtcgaacc cggctgagtt
      361 cgcgaaggga ttaggtagca gcgatccact ggtgcgacaa aaaagtgccg aggcgctcgc
      421 gaagcttgct gccaccgatc aacgtaagct agtcgaaggt tatcaacttc aggagaagaa
      481 caaagaagtc catctcgcac tcgactgggc gttgtatcgc atgggcaaag ccgaggcgct
      541 ctatcgcgtc gtcgacgatc tcgactccgg tcgccagcag caggccgtcg gctatttatc
      601 agagctcgaa agtcccgatg tcctttatcc gtttctgaag cgcgccaaca acgcgccgcg
      661 catcaacgcg ggattgctca aagccctcgc gcatatcggc gacgcgcaga cgctcgagct
      721 cgtcaagcct ttccgcgagt cgcatcagcc atatgtcgcc gaagccgctg agatcgccag
      781 cgacgaaatc gagaaacgcc tcagcgagcc tgccgcaaat gcaacccgcg cgcgcccgcg
      841 aactgttgag aaggctccgg atcgaccata agcttacgaa tgcacgccag cctgcgcaca
      901 tttctcgatc tactcaacag agaaaacgaa atcaccacta tcaaagccga ggtcgatcca
      961 tacctcgagc tcgccgagat ccatcgacgc gtgatcgctg aaggtgggcc ggcgttgctg
     1021 ttcgagcgtg tgaagggcag tcgttatccg gtcgtcacga acctgttcgg cacggagcga
     1081 cgcatagatc tggcgtttgg tccaaagccg gaagcgtttg tgaggcagat ggtccacatc
     1141 gcggagtcga ttctgccgcc gaagtttggt gagctgtggc agcatcgttc cgtcgcactc
     1201 gaactggcga aactcggaac gcgcaacacc tcgcggtcgc ccgtaacgca ggtggtcgat
     1261 cgtcctgccc gcctcgacga actgcccgtc ttgacgacat ggcaggaaga cggtgggcca
     1321 ttcatcacgc tgccgctggt ctacaccgag aacccgatca cgaagaaaca caatctcggg
     1381 atttatcgcg tccaggtcta cgactcgcac acgaccggcc tgcactggca gatccagaaa
     1441 ggcggcggct ttcattacag cgaagccgag cgtctcaatc agccgttgcc ggtgacgata
     1501 tttcttggtg ggccaccggc gctcattctc gccgcgattg ctccgttgcc tgaagacgta
     1561 cccgagcttg tgcttgcatc gttgctggcc ggacaaaaac tgaagatgac gcgcaacccg
     1621 ctcggcgaag ggcgacatcg tctcgcggca gaagcggagt ttgccctggt cggacatgcg
     1681 ccgccgcacc agcgtcgacc ggaaggacca ttcggcgatc attacggtta ctattcgttg
     1741 cgtcacgatt atccggtgtt caatgttgaa gcggtctttc atcgtcgcga tgcgatttat
     1801 ccggcgactg tagtcggcaa gccgcgccag gaagatttct tcatcggcga ctacttgcag
     1861 aaactattgt cgcccctctt tccgctcgtg atgccgtcag tgcgcgatct ctggacatat
     1921 ggtgagactg gatttcactc gctcgctgct gcggtggtgc aagaacgtta cggccgcgaa
     1981 gcgctggtct cgggttttcg aatactcggt gaagggcagt tgtcgctgac gaagtttttg
     2041 atactcaccg acacaccgca ggatctgaaa gactttccac gtttgttaga gtacgtgttg
     2101 gagcgcttcc gaccggaaac ggatctgttt gttttctcga atgtgtcgat ggacacgctt
     2161 gattacacga gtggcaaggt gaatgaaggc agtaaagcga tcatgcttgg attgggtgag
     2221 ggtgtgcgca aactgccgcg cgagtttaag ggagaattgc cggcgtcggt taagcgcgcc
     2281 gaggtgttct gtggtgggtg tttggtgttg gaaggcggag cgcgtgctga agatgtcgcg
     2341 aacgatccgg tgtttgctga gtggccattg atcgtcctgc atgatgatgc acgtgttgct
     2401 aaatcatcaa cggatttctt atgggccacg tggacgcgtt tcgagccggc gtcggatata
     2461 tcggctagac gaacggaagt gaggcggcat catctggttt acaccgcacc gatcgtgatc
     2521 gatgcgcgga cgaagcccgg gtttccggat gaattgattg tgcgggagga tataaaaacc
     2581 ctcgtcgatc agcgctggaa ggaatatttt agccacggat ttaaatccgg attttaaatc
     2641 cgtgtcatcc gtggctaagt tttatcgtaa acagtgcgcc gccgtcgggt gaattagtgg
     2701 ctgtcagagt tccgccgtgt tgcgtgatga tgcggtgagt gagcgcgagg ccgatgccgt
     2761 gaccttttgc tttcgtagtg aaaaaaggaa tgaagatctt ttgcagatcg gccggtgaaa
     2821 ttccggggcc ggtgtcccgt acctggatta cgacatttcc atcctcacgt gctgcactca
     2881 cagtaactaa tccatcagga gctgcttcag caccgttgcg gatgagattg agcagcgcca
     2941 gtcgcagcat tcgttcgtct gcgcgaatcg ttagtgacgg atccacgtca acgacaactt
     3001 tcaactcggt agcgcaatcc gcgatcagat ccgaaaggga tacttcctcg aggtgcaacg
     3061 gctgtgggcg cgcgaagttg agaaacgcgg tcaccatgcc cgagagattt cgcacttcat
     3121 taagcattga agcggccgca acctgcgcct tgtcgttcag ctccatgctc tgcaaaagct
     3181 gcgcgtaacc ctgcagcgtc gcgattgcat tcttgaactc gtgggccaac cccgccgaca
     3241 tctcgccgag actctcgaga ttgttcttca aagccacctg ttcgcgcagc tccgttactt
     3301 ccgtgatgtc cgtcaaaaga cagagcgcgc ctcttggtcc acgctcgggc ggcagatcga
     3361 tcggagcgac ggttgccccg agccgccgca gcttaccgtt ttcactgacg gcttcgatct
     3421 cggtgcgacg gtaaacggtt ccggtttgca aacagtcgcc aaccatcttc gcgagctctt
     3481 cgtgcgcgcc gaagagttgt ttgtacgact cgcctaacgc cgcgccgtta acttcgagca
     3541 gcgtccgtcc cggggtgttg atggtgatcg agagtcccgc accatcaaaa gcgattaatc
     3601 cggaaggcaa actggcgacg atacgctcgc tgaacttctc gagctcctta cgttgttcct
     3661 gaagctgcgc caccactgat tgaaacgtct cgagcacaaa ttcagcttcg tcctgtgatt
     3721 tgtgcggagc tgaaactgac gcgcgttgcg cttcgccgac aagctgttga tacggacgca
     3781 gcagccagcg cagtacaaac aaaatgcaca acaaacttcc gagcaggatc gcgatgaacg
     3841 cagcgagcag tatgggacta cgagcggcgg gagtttcagg cgtgaatgcg tagagcagaa
     3901 ccgcaaacag gactgccagc agaaacagtc ccgtgagcat cgtcagcccg gattggaaac
     3961 gattgcgata tgtcaccgga attggcctgc gtaccattcg accaacggcg cgccgaagag
     4021 cagtgcggcg attgcgccga gcccgagaaa gacaccgaag ggaagcagca tctgcatgtt
     4081 tcgctggccc tgcttcgcca tcaacaggat gccgatcagg gagccgctca acacgccgac
     4141 aaagatcgtt agaatcgtca gtcgccaacc tagatacgcg ccgaccataa acatcatctt
     4201 cacgtcgccc aggcccattg cttcgatgcc gcgcagtttt tcccacgtcc agcccatgag
     4261 ccagagcgag ccgccgccga tcaacgcgcc gatgaacgcg ccggcgagtg aaataaccca
     4321 cagcggcatt cctgcgagcg cgccgtgcga gagcgaaggt atgtcgtcga agtgcggcgt
     4381 gcctgttaat aaaggaatgg cgacgcgcgc gacgagtgca aacgcgatcc ccggatatgt
     4441 gatcacattc ggaagaatca tgtgttcggc gtcgatgaag acgagcgcga ggagcgcaga
     4501 agcgaagacc agatcaaacg gcaacgccgc actgagttgg tcgtgccagg cgacgccgac
     4561 aaacaatgcg gctgtcagca attcaaccgc gggataacga aacgagatgt gctctttgca
     4621 cttgcgacac ttcgcgccca ataacaggta actcagcacc ggaatgttgt cgtagaaagc
     4681 gatcaccgcg ccacacgaag gacagcgcga gtttggaaag acgatcgact cttcgttggg
     4741 aacgcgatga atgacgacgt tgagaaagct gccgatgatc gcaccaaaaa ctcccgcgat
     4801 cacataacca acaacagggg gaaccaggtt taaatcaatc atcttgatcc gtttttatcc
     4861 gtcttatcag cggcctgttt ttcccagagc atcaaatgct tcagaaacgc atgatgcgca
     4921 gcgaagctcg caatcgtaaa gcctgcgaaa ccgtctcgta aaccaccttt caaaatcaag
     4981 ctgcgaatga aggctgctgg gccagccgaa gcgacgccga tcacagacgt gcgacggcct
     5041 tcttcaaaca tctggcgagc ggccagtggt gcgtaacgct cgccgatcat gcggtgatga
     5101 tgcgcggcgt tttgtgaagt gtaatgcagc aggtcgccat tgagtcgctc gatgcgcgcg
     5161 cccggatcca tcgtcactga ctcgtgaatg tggcgttgtt tccattggcc cttgttcttc
     5221 cggaagagtc gtagctgtcg atcgggatac cagccgccgc cacgaatcca gcgtccctgg
     5281 tagtaagtgc ggcgagcaat ctcgtaaccg tccgcgagaa ttggtttttg ctttaacgat
     5341 tcgatcgatc ttttgagctc gtccgaaaca cgctcgtctg catcgagact gaagatccac
     5401 tcgtgttggg tttgttcgac tgcgaactgc ttctgtgcgc cgaaaccggg ccaggggttt
     5461 gtgatcactc gtgcgccgca gactgcggcg atttcgcgcg tcgcgtcagt tgaacccgag
     5521 tctaccacga ggatctcatc ggcccacgca actgactcgc aggcggcttt gatatttgat
     5581 tcttcgttga gggtgatgat ggtggcgctg atcttcatcc accgtaagct taccaaaacg
     5641 cacataaaaa gccgcggatt ttcgcggatg aacgctgatc tgcgtttatc tgcggaaatc
     5701 cgcggcaagt tttaggcgcg cgttgaacgc ttatggctga aggggcgtgt cgaccaccac
     5761 gttgttcgcc cgcagcgttg tcgatgtggc acgctgcaag tcggccagcg ccttgttgta
     5821 gtcagtttct gctcgcaact cagaagcacg cgcgttcgcg agtgcgttct cacgctggaa
     5881 cagaaggaac gtcgtcgaac gtcccacctg gtacaaacgc tgctcacctt cgagctgcat
     5941 ctccgcgttc gttcgcgctt cacgcgccga gagcacacgc tgtcgtgccg actcaaccgc
     6001 ctgagcagag ttacgaacat caacctcgat aacctgctcc tgcgatctca ccgtcgcctg
     6061 caactgctcc ttctgaattc gcgcgccggc aagattcgct tccgcggtct tgttcttgaa
     6121 cgggaattga atcgctacgc caagtgtgac gttgcgcgtt tcgccgccga acagattttg
     6181 caacgtctgc ccgtaaccac cgatcagctt cgggtttaca acatttgcgt ccacgacagc
     6241 caatggtggc accgccaccg ggagtccctg agccctttga atattttgga tctcattgag
     6301 caggaacgcc gatggatccg ttaacggatt gcctacaatc aaaggttgcg ttgcgcccgg
     6361 agggacaatg gttgcgaggt tgtttccagc cagacccgtg agcgcgacag tgccggtgaa
     6421 atcgatgcgc ggcttggttt
//
LOCUS       DASSFQ010000116         9184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_347997, whole genome shotgun sequence.
ACCESSION   DASSFQ010000116 DASSFQ010000000
VERSION     DASSFQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347997"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFH96_03880"
     CDS             complement(<1..608)
                     /locus_tag="VFH96_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6973137.1"
                     /translation="MLPALSPVASLRASLGHWWHTNQLWIAWTLLVVPLVLELIYGTF
                     LLPYLILTPISILATYLIYRSARARLQSKTSEIEALSELHLATAEALATAIDAKDQTT
                     HCHVRRVQIYAAGMGEVFGLSDEEIAALKAGALLHDIGKLAVPPHILNKPGPLTHAEF
                     EKMKIHTVVGAQILGRVDFPYPVIPIIRHHHEQWDGRGYPDRL"
     gene            899..2224
                     /locus_tag="VFH96_03885"
     CDS             899..2224
                     /locus_tag="VFH96_03885"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6973138.1"
                     /translation="MKTDHDSLPTHVGYIDLLRRNHSFRQLWLGQVVSQMGDWFDTIA
                     LYTIILRLTGSGRDVGLLLVARFVPSFFFGPISGVIADRFSRQRIMIVSDILRAFVVL
                     GFLFVRRPDQLWIIYVLTVFQLGLSTFFEPAKTAAIPSIVEDRELLAANAISSVTWSA
                     MLTIGAAIGGFITSLFGTDVAFIADAATYLLSAALIASIRLPKRKKRERQKVSLSRLL
                     GIGETIEGIRYVKDRPRVLAALLVKPAWGLGGGILTLLAVFGERIFPVGKDAAGGIGV
                     LFAARGIGTAVGPIVARRIAGEGNRRMQASIGIAFLIGGVFYILFGSATSFIFALVVL
                     GIAHCGGSILWVFSTVILQRSVVDSFRGRVFAAELALVTLTMAASNYVTGELLDRFQI
                     SPRIVTVGIGTFFLLPGIVWFITQRWWDREERVARVRVDEQQVVHQSIE"
     gene            2227..2781
                     /locus_tag="VFH96_03890"
     CDS             2227..2781
                     /locus_tag="VFH96_03890"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973139.1"
                     /translation="MKTKVGVMGSAAAESARLETGNTLVDKAERLAHVIAGKDVLLLT
                     GGTTGIVYVVGKTAHDAGSFHIGISPASNEREHSELYKLPTDACDALIYTGFGLKGRN
                     VVLVRSSDIVLFVSGAMGSLNEFTIAYDEGKIIGCLTGTGGVADESDYLMQKFGKRAS
                     MARVFTNEDPVKLLDDCMNALKSR"
     gene            2778..3410
                     /locus_tag="VFH96_03895"
     CDS             2778..3410
                     /locus_tag="VFH96_03895"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HET6973140.1"
                     /translation="MSSLIAHYINHPICPSDFDNQIWQECQPVPIGHYWSGDPAMPSR
                     HAEARVCWSDEALHVRFVGKQQEPLIVSENPITNAKTLGLWDRDVCEIFLAPDPAAPW
                     RYFEFEAAPTGEWIDLGITVKPEGRETDWEFASGLKTAAKLEDERLLVGMRIPWSDVL
                     PKPSAGDIWRANVFRCVGPEAPERYLAWLPTGTPEPNFHVPEVFGVLRFE"
     gene            complement(3454..3879)
                     /locus_tag="VFH96_03900"
     CDS             complement(3454..3879)
                     /locus_tag="VFH96_03900"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6973141.1"
                     /translation="MSELPRRIVSQLRRFIGNRRRDKRVRTRLIFTLSLSDPRVSTNG
                     SRRLPTLNGYTLDVSVTGLALIVPAIRIGEHYLAGADRKLHVKLELPGGPVEMKVVTV
                     RYEDLEDGSGYLIGARISEISDADRMSFEKYILAADSRG"
     gene            complement(3989..4660)
                     /locus_tag="VFH96_03905"
     CDS             complement(3989..4660)
                     /locus_tag="VFH96_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HET6973142.1"
                     /translation="MREFYRMMREKKFREAFGISIYRAAIEGLSTQEFDDLKPDFDRM
                     ALAVSEKIPEKVDITGEQISGDAATVFVKVVDADGKEKIEPASLIKIDNAWIVGDREN
                     LELVRKEGKKFFFEARINAHHSDVQDMLTRISLAQVVYAQGHNGQFGNMAELITGGLV
                     PKDIEGPETTGYRFQVFRATDGKSWYATAEPAQYGRSGRLSFFLDASGVRSGDSGGKP
                     LVVKN"
     gene            complement(4866..7256)
                     /locus_tag="VFH96_03910"
     CDS             complement(4866..7256)
                     /locus_tag="VFH96_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HET6973143.1"
                     /translation="MSSESIPANEYPDRVGFKPDNPYLTTFRPYIPAGVKLRELTILP
                     LFVGTVLGIIFGASSLYLVLKVGLTVSASIPVAVISITLFRVLSKFGLRDATILENNI
                     VQTAGSAGESIAFGIGVTMPAILILGFDLELSRVMLVSVLGGLLGILMMIPLRRALIV
                     AQHGFLKYPEGTACAEVLKAGASDESRAAALEGSRESAAVVDEAMTGGKTIISGFGIG
                     FLYYGIEQIFKTWKEYPGKVFGKPFEGGSVSIENNPALLGVGYIIGPRIASIMVAGGV
                     LAYLVLIPAIKYFGGGWMTPLAPETTHLIKDMSVTQIQKGYILYIGAGAVAAGGIISL
                     FRSLPVIWSGLKGGLADLRRSGTGSNDNAPRTDQDLSMKFVLAGIIALMVVIMIVPQL
                     NLRFNILGAILIIAFGFLFVTVSSRLTGEVGSSSNPISGMTVATLLLTCLVFLVIGWT
                     APPYFVTALSIGGIVCIAASNGGTTSQDLKTGFLVGSTPKYQQIAILVGALVSALALG
                     PILLAMNQSGSVYVPVAQNKDFSFPADFRFNPDQFVKSGDQPKKERLQGGQASADTTE
                     YNVVHKTTTDYGPAGRYFVNDQGVPVYLADPGINGIYDKVPGTNNTVAKFTAPKATLM
                     SYIIKGILSRELPWGLVLLGVMITIVLELSGVPSLAFAVGVYLPISTSAPIFVGGAVR
                     WLVDKYLRKKPENMNLSEEQMAAETDKSPGVLLASGYIAGATLAGVIFAFLNLREGIV
                     AQLKVFEEWATHHNPFFEGPNSDVLGMIPFLLLTILLYLVGRELVLKPRPIARREE"
     gene            7427..>9184
                     /locus_tag="VFH96_03915"
     CDS             7427..>9184
                     /locus_tag="VFH96_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HET6973144.1"
                     /translation="MRKRIITLLLLFLVTGVATAQKKILAPAEVLGFVPGEDRKLASW
                     DQIVDYFQKLDAASDRVVFETLGNTTMGKPFVMATISSPANLARLEEFKKIQDQLADP
                     RKLGPLATRDRKAAELIRKGRNVVLITCGIHSTEVGSYLSSTLIAHQLASSTDANVQK
                     ILDNTIVLLVPSLNPDGVDIVKNWYDKTLGTPFEGTDPPELYHKYTGHDNNRDWYAFT
                     QVETQITVDKILNVWHPQIVHDIHQQGSFGSRLFLPPYMAPVEPNVPKAIVQGYTELG
                     NWMAAEMRAKGFEGITTNSTYDAWTPARAYSHYHAGVRMLSETASARIASPMTLKFEE
                     LRSREGYDPQKESPKFGPLWRGGEWKLSDITKYMTTAAFLLLDHAATNREQWLRRFYA
                     IGKDAVRPRRAGELFGFLIEPSPTSEALLNILKRGGVEVSSAKGTVFKVGKQTLPSDT
                     QLVRMDQPYGAFAKALLEVQHYPNLRDADGHPIAPYDVTANTLPLLMGVPVQAVKAPF
                     RAVRSAEPVSAVLTDAPKTPRIDLFAIYHSSVPSQDEGWTRWMLDSKNISYGVLGDKE
                     LRAGITVYKPSPGVTGKYYT"
BASE COUNT         2031 a         2491 c         2637 g         2025 t
ORIGIN      
        1 aggcggtcag gataaccgcg accgtcccac tgctcgtggt gatgcctgat gatcggaatc
       61 actggataag ggaaatccac acgaccgagg atctgagctc cgacgaccgt gtggatcttc
      121 atcttctcaa actcagcatg agttagaggt ccgggtttat tcaaaatgtg cggcggaacc
      181 gcgagcttgc cgatgtcgtg tagcagcgca ccggctttga gtgcggcgat ctcctcatcc
      241 gagagaccaa acacttcgcc catgcccgcg gcataaatct gtacgcgacg aacatgacaa
      301 tgtgttgttt gatccttggc gtcgatcgcg gtggcgagtg cttctgcagt ggcgagatga
      361 agctcactga gcgcttcgat ctcgctcgtc ttcgattgca gacgtgctcg cgcgctgcgg
      421 tagatcaaat aagttgcaag tattgagatt ggcgtcagga tgagataggg caagagaaac
      481 gtgccgtaga tcaattcgag cacgagcggc acgacgagca atgtccacgc aatccacaac
      541 tggtttgtgt gccaccagtg acccagcgag gcgcgcagcg acgcgaccgg agacagagct
      601 gggagcaact cagctctctc tccggtgaat tgggcaagtg gcaacccgat acggtcgggt
      661 tgccgtgctg ggtgagatgt ttgacggctc gttgatgtca tagaactctg cggctacggg
      721 ccccgtgcgg agggcccgcg aagccagtgg cttctgcgcc gcgctgtccc ctagttcaac
      781 tgtcgtgccg ttttaaattt ccaagaattc gcgattgttg gtggtgagtc attccattct
      841 gacaggtcag attgagaatt tcagtcaggc tggttactct gggccgcgga ttaaacggat
      901 gaagacggat cacgattctt tgccgacaca tgtcggatac atcgatctgc tgcggaggaa
      961 ccatagcttt aggcaactgt ggctcgggca ggtcgtcagc cagatgggcg actggttcga
     1021 caccatcgcg ctctacacga tcattcttag acttacgggc tcgggtcgcg acgtcggtct
     1081 gctgctcgtc gcgcgcttcg ttcccagctt cttcttcgga ccgatctcgg gcgtgattgc
     1141 tgaccggttc agccgccagc gaatcatgat cgtatcagac attctgcgcg cgtttgtcgt
     1201 attgggattt ctgttcgtgc gccgtccgga tcagttatgg atcatctacg tgctgacggt
     1261 ttttcaactc ggactctcga ctttcttcga gcccgcgaag acggcggcga taccatcgat
     1321 tgtcgaggat cgtgagctac tggcggcgaa tgcgatctcg tcggtgacct ggtcggcgat
     1381 gctcaccatc ggcgctgcca tcggaggctt tatcaccagc ttgtttggaa ctgatgtcgc
     1441 gttcattgcc gatgccgcga cgtatttgct gtccgcggcg ttgattgcga gtattcgtct
     1501 tccgaaacgt aaaaaacgcg agcgacaaaa ggtgtcgcta agccggcttt tggggattgg
     1561 ggagacgatt gaaggtatca ggtatgttaa ggatcgaccg cgtgtcctgg cagcgttgct
     1621 ggtaaaacca gcgtggggac tcggcggtgg catactgacg ctgctggcgg tgtttggcga
     1681 acggattttc ccggttggca aagatgcggc aggcggaatc ggcgttcttt ttgcagcgcg
     1741 agggatcgga accgctgtcg gtcccatcgt cgctcgccgg atcgcgggcg aaggaaaccg
     1801 gcgcatgcag gcgtcgatcg ggatcgcgtt tctgattggc ggtgtttttt acatcttgtt
     1861 tggcagtgcg acgagtttta tttttgcgct cgtcgtgttg ggaatcgcac actgtggcgg
     1921 cagcattctc tgggtctttt ccacggtgat attgcaacgg tcggttgtgg atagctttcg
     1981 cgggcgcgtg tttgcggcgg agctggcgct ggtgactttg actatggcag cgtctaatta
     2041 cgtcactggc gaactgttgg atcggtttca gatctcgccg cggatcgtga cagttggtat
     2101 tgggactttt tttctattgc cgggtattgt gtggttcata acgcagcgtt ggtgggatcg
     2161 tgaagaacgt gtggcgcgtg taagagttga cgagcaacag gttgttcacc agtcgattga
     2221 ataactatga agactaaagt tggagtgatg ggttcggctg ctgctgagag cgcgcggctc
     2281 gagaccggca acacgctggt cgataaggcc gagcggctcg cgcacgtgat tgccggcaag
     2341 gatgtgttgc tgctaacggg cgggacgacg ggcatcgttt acgtggttgg caagacggcg
     2401 cacgatgcgg gtagttttca tatcggcatc tcaccggcga gcaatgaacg cgagcatagc
     2461 gagttgtaca agttgccgac ggatgcgtgc gatgcgttga tctacacggg ttttgggttg
     2521 aaagggcgaa atgtcgtgct cgtgaggtcg agcgatatcg tgctgttcgt gtcgggcgca
     2581 atgggatcgt tgaatgagtt cacgattgcg tacgatgagg gaaagattat cgggtgtttg
     2641 accgggacgg gtggtgttgc tgatgagtca gactacttga tgcaaaagtt tgggaagagg
     2701 gcgagtatgg cgcgtgtgtt tactaatgaa gatccggtga agttgttaga cgactgcatg
     2761 aacgcgttga aatcgagatg agctccttaa tcgcgcacta catcaaccac ccaatttgcc
     2821 cttccgactt tgataatcaa atttggcagg agtgtcagcc cgtaccgatc gggcattact
     2881 ggtccggtga tccggcgatg ccttcgcgcc acgctgaagc gagagtctgc tggtccgatg
     2941 aagcgctgca cgtgcggttt gtcgggaaac agcaggagcc gctgatcgtc tcagaaaatc
     3001 caatcacaaa tgcaaagaca ctcggcctct gggatcgcga cgtctgcgag atctttctcg
     3061 cgccggatcc agcagcgccg tggcggtatt ttgagtttga agcagcgccg actggcgagt
     3121 ggatcgatct tggaatcaca gtaaaaccag aaggaagaga aactgattgg gaattcgcgt
     3181 caggattgaa gactgctgcg aagctcgaag acgagcgatt actcgtcggg atgcgaattc
     3241 cgtggagtga cgtgcttcct aagccatcgg cgggcgatat ttggcgagcg aacgtgtttc
     3301 gttgtgtcgg cccggaagcg ccggagcgtt acctcgcgtg gctaccgaca ggaacaccag
     3361 aacctaactt tcacgttccc gaagtcttcg gagtgctgcg cttcgagtag aagtttaagc
     3421 cgcggattta cgcagatgaa cgcagatctg cgtttatccg cgtgaatccg cggctaaaat
     3481 atacttctca aaactcatcc gatccgcgtc gctaatctcc gaaatccgag ccccgatcag
     3541 gtacccactt ccatcttcca gatcctcata cctgaccgtc accaccttca tctcgactgg
     3601 tccacccggc agctccagct tcacgtgcag cttccgatca gccccggcaa ggtagtgttc
     3661 accgatccga attgcaggaa caatcagcgc cagccccgtg acactgacgt ccaatgtgta
     3721 cccgttgagc gtgggtaaac gccgggaacc gttcgtgctc acacgcggat cagacaagct
     3781 cagcgtgaaa atcagacgtg tgcgcacacg cttatctcgc cgccggtttc caataaaacg
     3841 gcgcaattgc gacacgattc tgcgtggtag ctccgacatc cttcgcaacc actaaaaaga
     3901 tttctcggac caaatttgag gagggcacga tcgggggcag ttcgccgcac aagcataata
     3961 gaaaacctgc tcgcgcggac gcaacacttt aattctttac tactagaggt ttgccgccgg
     4021 aatcgccgct acgcactccc gaagcgtcga gaaagaacga aagccggccc gagcgaccat
     4081 actgggcagg ttcggcggtc gcataccacg actttccgtc cgtggcgcgg aacacctgaa
     4141 agcgatagcc ggtagtttcc gggccttcga tgtctttcgg aaccaggccg ccagtgatga
     4201 gctcagccat gttgccaaac tggccgttgt ggccctgcgc gtagaccacc tgcgcgagcg
     4261 agatgcgcgt caacatgtcc tggacgtcgc tgtggtgggc attgatacgg gcttcgaaga
     4321 aaaacttctt gccttccttg cgcaccagct cgaggttctc gcgatcgcct acgatccagg
     4381 cgttgtcgat cttgatcagt gaggcgggct cgatcttttc cttgccatcg gcgtcgacta
     4441 ctttcacgaa gacggtcgcg gcgtcgccgc tgatctgttc gccggtgatg tcgaccttct
     4501 cgggaatctt ctcgctgact gcgagggcca ttctgtcgaa atcaggtttc agatcgtcga
     4561 actcctgcgt cgagaggccc tcgattgccg cgcgatagat cgagataccg aatgcttcgc
     4621 gaaacttctt ctcgcgcatc atgcggtaaa actcgcgcat tgtttcagtg ggtgtacgcg
     4681 cgatttgtgc gaatgaggtg gcagataaaa gaagtgtcag cagaaaaacg ctaataactc
     4741 gaatacgcat atagtttggg cggtcaaggg acaactgtag gggtggccct ctgtggccac
     4801 ccgctccgtg aacctatcgc acgggcggcc atagaggacc gcccctacag ttgtcgatta
     4861 cgtgttcatt cttctctgcg agcgattggg cgtggtttga gtaccaactc ccgcccaaca
     4921 agatacagca gaatcgtgag tagcaggaac ggaatcattc ccagcacgtc tgaattcggg
     4981 ccttcgaaga acggattgtg atgcgtcgcc cactcctcga aaacctttag ctgcgcaaca
     5041 atgccttccc tcaaattcag gaacgcgaag atcacgcccg cgagcgtcgc accggcgata
     5101 taacccgacg cgagcagcac gcccggactc ttatcagtct cagccgccat ctgctcttcg
     5161 ctgagattca tattctccgg cttcttccgc aggtacttgt ccaccagcca acgcaccgcg
     5221 ccgccaacaa agatcggcgc cgacgtcgaa atcggcaggt aaacaccgac cgcgaaagcg
     5281 agcgaaggca caccgctcaa ctcgagcaca atcgtgatca tcactccgag caacacaagc
     5341 ccccacggca gctcacgact cagaattccc ttgatgatgt aggacatcaa cgtcgccttc
     5401 ggcgcggtga acttcgcaac ggtgttgttc gtgcccggca ccttgtcgta gataccgttg
     5461 atgcctggat ccgcgagata aaccggcacg ccctgatcgt tcacgaaata acgtcccgcc
     5521 ggaccgtaat cggtggtcgt cttgtgcacg acgttgtatt cagtcgtatc agcgctcgcc
     5581 tgtccgcctt gcagccgctc tttcttcggc tgatctccgc tctttacgaa ctgatcggga
     5641 ttaaacctga agtccgcggg gaaactgaaa tccttgtttt gagccaccgg cacgtaaacg
     5701 ctgcccgact ggttcatcgc cagcaggatt ggtccaagcg cgagggcgga aacaagagcg
     5761 ccgacgagaa tcgcgatctg ctgatacttc ggtgtagaac cgacgagaaa gccggtcttt
     5821 aagtcctgag acgtcgttcc gccattcgac gcagcgatac acacgatgcc gccgatcgaa
     5881 agcgcggtta cgaagtacgg cggtgcggtc caaccgatca ccagaaacac gagacacgtc
     5941 agcagcagcg tcgcgaccgt cattcccgag atcgggttcg acgacgatcc gacttcaccc
     6001 gttagccgcg acgagaccgt tacaaataga aatccaaacg cgatgatcag aatcgcgcca
     6061 aggatgttga agcgcagatt gagctgcggg acgatcatga tcacgaccat taacgcgatg
     6121 attccggcga gcacaaactt catcgaaagg tcctggtccg tgcgtggcgc gttatcgttg
     6181 gagccagtgc ccgatcgtcg caagtcagca aggccgcctt tcaaaccgct ccaaatcacc
     6241 ggcagcgatc tgaacagact gatgattcca cccgctgcga ctgcgcccgc gccgatgtaa
     6301 aggatgtagc ccttttgaat ctgcgtgacg ctcatgtctt tgatcaggtg tgtagtttcc
     6361 ggagcgagcg gcgtcatcca gccgccgccg aaatacttga tcgcgggaat caagacgagg
     6421 tatgcgagca cgccgcccgc aaccatgatc gaggcgatgc gcggtccgat gatgtaaccg
     6481 acaccgagca gcgcgggatt gttttcaatc gagactgagc caccttcaaa tggtttgccg
     6541 aagactttgc cgggatactc tttccacgtc ttaaagatct gttcgatacc gtagtaaaga
     6601 aaaccgatac caaagccgct gatgattgtc ttgccgccag tcatcgcttc atcgacaacc
     6661 gcagcgcttt ctcgcgatcc ttcgagcgcg gcggcgcgcg attcgtcgga agcgccggcc
     6721 ttcaagacct cggcgcaggc ggtgccttcg ggatatttta gaaatccgtg ttgagcgacg
     6781 atgagcgcgc gtcgcaaagg aatcatcatc aggatgccga gcaggccgcc aagcaccgaa
     6841 accagcatca cgcgcgataa ctcgagatcg aatccgagaa tcaaaatcgc cggcatcgtg
     6901 acgccgatac cgaaggcgat cgactcaccg gccgaacctg ctgtctgaac gatgttgttt
     6961 tcgagaatcg tagcgtcgcg taaaccgaat ttcgataaca cgcggaaaag cgtgatggag
     7021 atcaccgcca ccggaatcga cgcactgacc gttagaccaa ccttgagcac gagataaagc
     7081 gaggacgcac cgaagatgat gccgagtacc gttcccacga acagcggcag tatcgtcaac
     7141 tcacgcagtt tgacgcccgc cgggatgtac ggtcgaaaag tcgttaaata cggattgtct
     7201 ggcttgaaac cgacccgatc tgggtactcg tttgcaggga tgctttcact ggacatccga
     7261 attgacctcc aacaagtttt gaaaaacggg gagtgcgcag gggttagaaa acgccgttac
     7321 tttaccatga atgcagtgtt tttgaacctg attaggcagt ctgaggttgc cttatagatc
     7381 ctataggact aagagggcat attcttttgc taaactgctg caacccatgc gcaaacgaat
     7441 aatcacactg ctgctgctat ttctcgtgac tggggtcgcc acggcccaga aaaagattct
     7501 ggcgcccgct gaagtcctcg gcttcgtgcc cggcgaggat cggaaactcg cctcctggga
     7561 ccagatcgtc gattactttc agaaactcga cgccgccagc gaccgggtcg tcttcgaaac
     7621 gctcggcaac acgactatgg gcaagccgtt cgtgatggcg accatcagct cacccgcaaa
     7681 tctcgcgcgc ctcgaagagt ttaagaagat tcaggaccag ctcgccgatc cacgcaagct
     7741 tggcccgctc gccacacgcg atcgcaaagc agcggagctg attcgaaagg gcaggaacgt
     7801 cgttctgatc acttgcggaa tacactcgac tgaagtcggc tcgtatcttt cgagcacact
     7861 gatcgcacac cagctcgctt cgtcaaccga cgcgaatgtg caaaaaattc tcgacaacac
     7921 gatcgtcctg ctcgtcccat cgctgaatcc cgatggcgtc gatatcgtca agaactggta
     7981 cgacaaaacc ctcggcacac cattcgaagg caccgatccg cccgagctct atcacaagta
     8041 cacgggtcac gataacaacc gcgactggta tgccttcacg caggtcgaga cgcagatcac
     8101 tgtcgacaag attctcaacg tctggcatcc gcaaatcgtc cacgacatcc accagcaagg
     8161 ctcttttgga tcgcgtctgt tcctaccgcc ttacatggcg ccggtcgagc caaacgtgcc
     8221 aaaagcgatc gtgcagggtt acaccgagct cggaaactgg atggctgccg agatgcgcgc
     8281 caaaggtttc gaaggcatca ctacgaattc aacctacgac gcatggactc ccgcgcgcgc
     8341 gtattcgcac tatcacgcgg gtgtacgaat gttgtctgaa accgcgtctg cgcgaatcgc
     8401 gtcgccgatg acgttgaagt ttgaagagct tcgttcgcgc gaaggctacg atccgcaaaa
     8461 ggaatcgccg aagtttggac cactgtggcg cggtggtgag tggaaattga gcgacattac
     8521 gaaatacatg acgaccgccg cgtttctgtt gttggaccac gcggcgacga atcgcgagca
     8581 gtggttgcga cgtttttatg cgatcgggaa ggacgcggta cgaccacgcc gagccggtga
     8641 gctttttggt tttttgatcg agccgtcacc aacgtccgaa gcgctgttga atattctgaa
     8701 acgaggcggt gttgaagttt cgtccgcaaa aggaacggtt ttcaaagtcg gcaagcagac
     8761 tcttccgagt gatacacaac tggtccgcat ggatcagcct tatggcgcgt ttgcgaaagc
     8821 gttgctcgaa gttcagcatt atccaaatct cagagacgct gacgggcatc cgattgcgcc
     8881 gtacgacgtg accgcaaaca cattgccgtt gttgatgggc gtgccggtgc aggctgttaa
     8941 ggcgccattt cgtgcggtgc gaagcgcgga accagtttcg gctgttttga ctgacgcacc
     9001 caagacgccg cgtatcgatc tgtttgcgat ttatcactcg tctgttccga gtcaggatga
     9061 aggttggacc aggtggatgc tcgattcgaa gaacatctcg tacggcgtgc tcggtgataa
     9121 ggagctgcgc gcgggcatca ctgtttataa gccgtcgccg ggtgtgacgg ggaaatacta
     9181 caca
//
LOCUS       DASSFQ010000117         3517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1381407, whole genome shotgun sequence.
ACCESSION   DASSFQ010000117 DASSFQ010000000
VERSION     DASSFQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381407"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1788)
                     /locus_tag="VFH96_03920"
     CDS             complement(46..1788)
                     /locus_tag="VFH96_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973145.1"
                     /translation="MEDLKKFALYFGKYRPQLILGIACIFASVVAGLFIPRIVGQAVD
                     ANWTEVSWSKLTISAGKVLGASLISGVFLFLQRRILIGMSRNVEYDLREDLYQHLVDQ
                     PLSFFHEHRTGDLMARATNDLSAVRQLAGPVIMYALQTVFVVVLVLPFLFMLNRWLTL
                     LLFVTMPLVSLTVKFFGQQVHTRFEKIQDFFGQITARAQENFSGVRVVRAYAQEEAEM
                     AAFNTLNWQYAERNLGLVKVEALMRPLMTFLIGFGYVLIIWAGVPLAVRGEITVGEFT
                     MFNLYLTRLIWPLIALGYVVNLYQRGTASLKRLNAILRVEPSIADRPGTVEQPPIKGK
                     IEFRDLTFRYHSNDEPVLKDLTLTIPEGHTVAFVGRTGSGKSTIANLIPRLIEAPAST
                     VFIDGVDIRDYPLAQLRESIGYVPQETFLFSDSVKGNIAFGVKKAEMAEVEWAAEIAG
                     LSDDIAGFPDRFEALVGERGITLSGGQKQRTAIARAVLREPKILILDDALSSVDTYTE
                     EKILGRLRGVMRDRTSVIISHRISTVREADMICVLDEGRIIEQGTHEELLTLGGEYAE
                     LYERQLLEEELAAT"
     gene            complement(1808..2188)
                     /locus_tag="VFH96_03925"
     CDS             complement(1808..2188)
                     /locus_tag="VFH96_03925"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6973146.1"
                     /translation="MTLDHVPFARLLGIEVDSVEPGHAVLSMQLQHEHMRNNAIAHGG
                     VIATLIDSAMAIAIMALLEENERTVTVDLTIHYLRPVPEGSAKASARVVRAGRRVVTV
                     SAELFDADGKLAATAISTYLRGLT"
     gene            complement(2185..2595)
                     /locus_tag="VFH96_03930"
     CDS             complement(2185..2595)
                     /locus_tag="VFH96_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HET6973147.1"
                     /translation="MSDSMQQRVPYAVSPPRYSVSHQMVTTAFELPNFRVLQNLGVVR
                     GIVVRSRNVFATLGATLQTIVGGNITVWTTLCEQTRNDAFDIMIQHATEIGANAIIGA
                     RYDTTELSQGVTEVLAYGTAVIVEPTNPGESYRS"
     gene            complement(2641..3027)
                     /locus_tag="VFH96_03935"
     CDS             complement(2641..3027)
                     /locus_tag="VFH96_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET6973148.1"
                     /translation="MAETKQSNPKYRERVYAIVRRIPSGRVMTYGQIAELLGDGYTPR
                     TVGFCMHSSPEGTPWHRVLNAQGKTSTGRIVLPHDKQQRMLEQEGVAFDKNGRCDLEK
                     FLFIPKTRSQNVSLYSHKRHKKHKKD"
     gene            complement(3110..>3517)
                     /locus_tag="VFH96_03940"
     CDS             complement(3110..>3517)
                     /locus_tag="VFH96_03940"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6973149.1"
                     /translation="EVPDDLHAISEEFVLANCAPDTTRVLFKTRNSAFWSESEPEFHT
                     DFTYLDLNAAQRLVERGIKLVGIDYLSIEKYGSSNHATHLALLSKGVVILEGLNLTDI
                     PPGNYELICLPLRLRNSKGDGAPARVVVRTLDL"
BASE COUNT          803 a          981 c          941 g          792 t
ORIGIN      
        1 cacagattac acagaggttg atctgtgtaa tctgtggatg gcttttcagg ttgccgccaa
       61 ttcctcttcg agtaactgtc gttcatacaa ttccgcatac tcaccaccga gcgtcaacaa
      121 ctcctcatgc gttccctgtt cgatgatgcg gccttcgtca agtacgcaga tcatgtcagc
      181 ttcgcgcacg gtcgaaatac gatgcgagat gatcacgctc gtgcggtcgc gcatcacgcc
      241 acgaagacgg ccgagaatct tctcttccgt gtacgtatca actgacgaca acgcgtcatc
      301 gagaatcagt atctttggct cgcgcaaaac agcacgcgca atcgcggtgc gttgcttctg
      361 cccgcctgac aacgtgatgc ctcgctcacc gacgagcgcc tcaaaccgat ccggaaaccc
      421 ggcaatatca tcagacaaac ccgcgatctc cgcggcccac tcaacctccg ccatctccgc
      481 cttcttgacg ccgaacgcga tgtttccctt caccgagtcg ctgaacagaa acgtctcctg
      541 cggcacgtaa ccaatactct cgcgcagttg cgcgagcggg taatcacgaa tgtcgacccc
      601 atcaatgaac accgtactcg ccggcgcttc gatcaaacgc ggaatcagat tcgcgatcgt
      661 cgacttcccg cttcccgtgc gtccgacaaa agcaaccgtg tgaccttccg gaatcgtgag
      721 cgtcagatcc ttcaaaacag gctcgtcatt cgagtgataa cgaaacgtca gatcgcgaaa
      781 ctcgatcttt cccttgatcg gcggctgctc gaccgtgccg ggacgatcgg cgatcgaagg
      841 ctcgacgcgc aggatcgcat tgagtcgttt caaactcgcc gtgccgcgtt gataaagatt
      901 cacaacgtat ccgagcgcga tcagcggcca aatcagtcgc gtgagataca aattgaacat
      961 cgtgaactcg ccgacagtga tctcaccacg cacggcgagc ggcacgcccg cccagataat
     1021 caacacgtaa ccaaacccga tcagaaacgt catcaacggc cgcatcaacg cttccacttt
     1081 tacaagcccg agattgcgct cggcatactg ccagttgagc gtgttgaagg ccgccatctc
     1141 ggcttcttcc tgcgcatacg cacgcactac gcgcacgccg ctgaagtttt cctgtgcgcg
     1201 cgcggtgatc tgtccgaaga aatcctggat cttttcgaag cgtgtgtgga cctgttgtcc
     1261 gaagaacttt acggtcaacg aaacaagcgg catcgtgacg aagagcaaaa gcgtcagcca
     1321 ccggttcaac atgaacagga acggcaggac cagaacaacg acgaacacag tctgaagcgc
     1381 gtacatgatc acgggtccgg cgagttgtct caccgccgat agatcattcg tggcgcgcgc
     1441 catcagatcg ccggtgcgat gttcgtggaa aaacgagagt ggctgatcga cgaggtgttg
     1501 atataaatcc tctcgcaggt cgtattcgac attacgcgac atgccgatca gtatgcggcg
     1561 ttgcaggaat agaaagacgc cgctgatcaa actcgcgccc agcaccttgc cggccgagat
     1621 cgtcagcttt gaccaggaaa cttccgtcca gtttgcgtcg acagcctgac caacaatgcg
     1681 tggaatgaag aggccggcta cgacactggc gaaaatgcag gcgataccga ggattagttg
     1741 aggtctgtac ttcccaaaat atagtgcaaa cttctttaga tcttccacgt gagggataat
     1801 actttatcta cgtcaatcca cgtaaatatg tactgattgc agtcgctgcg agctttccgt
     1861 cagcgtcgaa aagctctgcc gaaactgtga ctactctgcg tccggcgcgc acgactcgcg
     1921 ctgatgcttt cgccgaacct tccgggaccg gccgcaaata atgaatggtg agatcgaccg
     1981 tcactgtgcg ttcgttttcc tcgagcagcg ccatgatcgc gatcgccatc gccgagtcaa
     2041 tgagagtagc gatgacgccg ccgtgcgcga tcgcgttgtt gcgcatgtgc tcgtgctgga
     2101 gttgcatcga gagcacagcg tgaccgggct cgaccgagtc gacctcgatg ccaagcaacc
     2161 tggcaaatgg aacatgatcg agcgtcatga acgataactc tctcccgggt tcgtcggctc
     2221 aacgatcacg gccgtgccgt aagcgaggac ttccgtcacg ccttgcgaga gctcggtcgt
     2281 gtcgtaacgc gcgccgatga tggcattcgc gccgatctcg gtcgcgtgtt gaatcatgat
     2341 gtcgaaagcg tcgttgcgcg tttgttcgca gagtgtggtc caaacggtga tgttgccgcc
     2401 gacgatcgtc tggagtgtcg ctcccagcgt ggcgaagacg tttcgcgagc gcaccacgat
     2461 gccgcgcacg acgccgagat tctgcaagac gcgaaagttc ggcagctcga aagcggtggt
     2521 taccatctgg tgcgacacgg aataacgcgg cgggctgaca gcgtaaggaa cgcgttgttg
     2581 cattgagtct gacatcggct tctccttagc cacaaaaggc acaaaaagca caaaagaaaa
     2641 ttagtccttt ttgtgctttt tgtgcctttt gtggctatac aggcttacgt tttgagatct
     2701 ggttttgggg atgaacagga atttttcgag gtcgcagcga ccgttcttat caaatgctac
     2761 gccttcctgt tcgagcatgc gttgttgttt gtcgtgtggc agaacgattc ggccggtgga
     2821 agttttaccc tgtgcattta gcacgcgatg ccacggcgtg ccttcgggcg acgagtgcat
     2881 gcagaagccg acggtgcgtg gcgtgtagcc gtcgccgagc agctcagcga tctggccgta
     2941 agtcatcact cggcctgaag gaatgcgacg cacaattgcg taaacgcgct cgcgatattt
     3001 agggttgctt tgtttagtct cagccacttt atcttgttaa tcatgtgaat cctgtaatcg
     3061 tgtctaaaac taacgtgcgt tagagacagg attacaggat tttcaggatt tacaaatcta
     3121 acgttcgcac gacaactcgc gctggtgctc catctccctt gctgtttctt aaacgcaagg
     3181 gcaggcagat gagttcatag ttgccgggcg gtatgtcagt caggtttaag ccttcaagaa
     3241 tcacgacgcc tttcgacagc aatgccagat gtgtcgcgtg gttcgaggag ccatactttt
     3301 cgatcgatag atagtcgatg ccgacgagtt ttattccccg ctcgacgagt cgctgcgccg
     3361 cattaagatc gagataagtg aagtcggtat gaaactcagg ctcgctctca ctccagaatg
     3421 cggagttgcg ggttttgaag agtacgcgcg ttgtatccgg agcgcagttt gccagaacaa
     3481 actcttcact gatggcgtgc aaatcgtctg gcacttc
//
LOCUS       DASSFQ010000118         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_943472, whole genome shotgun sequence.
ACCESSION   DASSFQ010000118 DASSFQ010000000
VERSION     DASSFQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943472"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VFH96_03945"
     CDS             complement(<1..1027)
                     /locus_tag="VFH96_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6973150.1"
                     /translation="MHPSTLAKRVYLLLFAIIIAFYLYGLGKLPLLGPDEPRYAQVAR
                     EMFVTGDLITPTLGHHTWFEKPALLYWMIAGSFKVFGVNEWAARFGPALCGLLTIAAV
                     WFVGREIDREEPRGFAFWSLVVSGSCLGLIVFARAASFDVVITMTTSWALAFFLLHEL
                     RNKRSFLAGFYVFVGVSLLAKGLVGIVIPFGVVGLYYVLRRTWPSRSMILSLFWGMPL
                     ALAVSAIWYGPVIARHGWTFIDEFFVQHHFARYVSNKYHHPQPIYFYPVIILMLALPW
                     TPYLIAALAKVRSWKWRGDDSLNIVRVFSLAWLLLPIIFFSFSGSKLPGYVLPVIPGI
                     ALLVSDRL"
     gene            1136..2122
                     /locus_tag="VFH96_03950"
     CDS             1136..2122
                     /locus_tag="VFH96_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6973151.1"
                     /translation="MLRTAVIGVGSLGRQHARIQADLAAEGAAQFVAVCDLNEQTARS
                     VSSERETDWTTDWRELIGRIDAASVAVPTEAHCEIACGLLEAGIHVLVEKPISRTLDE
                     ADQMIDAAKRGNAILQVGHLERFNPAMVALKPHVRKPVYFEIHRVGQFTARSLDIDVV
                     LDLMIHDLDIVQWLVGEDVEVTSIHAVGIAILTNKVDAANARLEFSSGAVANITASRV
                     GTEKIRKMRFFQPHDYIAIDYTTKRASVSSLAPPGASGAWPGVHVKHLDIIDVEPLRA
                     EIVSFLEAARDGAPPAVSGIEGRNALSLALRTLERIREHAEHPGVKPQITRG"
     gene            2124..3260
                     /locus_tag="VFH96_03955"
     CDS             2124..3260
                     /locus_tag="VFH96_03955"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6973152.1"
                     /translation="MPVRNDQLSALLQQHIEAGEFPSAVYLVGERDQVIYEDALGNSV
                     VEPYRVANKIDTIYDLASLTKPLVTALLCARRIELGELTLDSSVSHYLPEFDRTDKQM
                     ITIRELLTHTSGLPAWRPLYVLTEEQPDRAAGAIASLDLEYKPGTRVVYSDLGFIALG
                     ILLERMMHQHLADMAKHEIFEPLKLQQTFFNPEIALQTGIAACETGNAYELDMSKQMG
                     AGEYANSRQRLIWGEVHDGNAYFLGGAAGHAGLFSTARETFQMTQQFLSESTTLLAPE
                     TCRLFRENMTPGLEEARSIGWQLAATKDSTGGTDLPPDSFGHNGFTGTCVWIDPSRRR
                     VFILLTNRTHARALPFANINAVRRQFNTLAADITDKKADLYLEP"
     gene            complement(3243..>3533)
                     /locus_tag="VFH96_03960"
     CDS             complement(3243..>3533)
                     /locus_tag="VFH96_03960"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HET6973153.1"
                     /translation="FERRFRRPGINVTRYRNRLQVEHPVGPSKWGLSLYVADEVFYDW
                     SIDRWVRNRFTVGGSKVFNKHFTQDIYYLRQNDGVSIPGDLNVIGTTLRLKI"
BASE COUNT          860 a          987 c          999 g          687 t
ORIGIN      
        1 tcaaacgatc gctcacgagc aacgcaatcc cgggtatcac cggcaacacg taacccggca
       61 gcttcgatcc tgaaaacgaa aagaaaatga tcggcaacag cagccaggcg agtgagaaaa
      121 cgcgaacgat gttcaacgaa tcgtcaccgc gccacttcca gcttcgcact ttcgcgagcg
      181 cggcgatgag atacggcgtc cacggcaacg cgagcatcag gatgatcacg ggatagaaat
      241 agatcggctg cggatggtga tacttgttcg acacgtaccg cgcaaagtga tgctgcacaa
      301 aaaactcgtc aataaacgtc cagccgtgtc gggcaataac tggtccatac cagatcgcgg
      361 agactgcgag cgccagaggc attccccaaa acaaactcaa gatcatggat cgcgacggcc
      421 acgtgcgtct caagacgtag tacaaaccaa caacaccaaa cggaatcacg atccccacga
      481 gcccttttgc caacaacgac acgccgacaa acacataaaa gccggccaga aacgaacgtt
      541 tatttcgtaa ttcgtgtagc agaaaaaacg cgagtgccca cgaagtcgtc atcgtgatca
      601 caacgtcgaa actcgccgcg cgtgcgaaca cgatcaaccc gagacaactg ccactcacca
      661 ccaaactcca aaacgcgaaa ccgcgcggct cctcgcgatc gatctcgcgt ccgacaaacc
      721 acaccgccgc gatcgtcaac agtccacaca gtgccgggcc aaagcgcgcc gcccactcat
      781 tcactccaaa gactttgaac gaacccgcga tcatccagta aagcagcgcc ggcttttcaa
      841 accaggtgtg atgtccgagc gtcggagtga tcagatcgcc ggtgacaaac atctcgcgcg
      901 ccacctgtgc gtaacgcggc tcgtccggac caagcagcgg cagtttgccg aggccgtaaa
      961 gataaaaggc gatgatgatg gcgaaaagga gaaggtagac tcgcttggca agcgttgaag
     1021 gatgcaagta tattcgggct ccgagtcagt tgtttacagg attcacaaga ttagacaaga
     1081 tttacatgct aatcctgaaa aatcttgtaa atcctgtcaa aggttttaac acataatgct
     1141 acgcacagca gtcattggag tcggcagtct ggggcgacaa cacgcccgca tccaggcaga
     1201 tctcgcagcg gaaggcgccg cgcaattcgt cgccgtttgc gacctaaacg aacaaacagc
     1261 gcgatccgtc agcagcgaac gcgaaactga ttggaccaca gactggcgcg agctgatcgg
     1321 tcgtatcgac gccgcgagtg tcgctgttcc aactgaagca cactgcgaga tcgcgtgcgg
     1381 cctgctcgaa gccggcattc acgtgctcgt tgaaaaaccg atctcacgca cgctcgacga
     1441 agccgatcaa atgatcgatg ccgccaaacg cggtaacgcg attcttcagg tcgggcatct
     1501 cgaacgcttc aatcccgcga tggtcgcgct gaaaccgcac gtgcgcaaac cagtttattt
     1561 cgagattcat cgcgtcgggc agttcactgc gcgctcgctc gacatcgacg tggtgctcga
     1621 cctgatgatt cacgatctcg atatcgtgca gtggctcgtc ggcgaggacg ttgaagttac
     1681 aagcattcat gcggtgggaa tcgcgatcct gacgaacaag gttgacgcgg caaatgcgcg
     1741 actcgaattc tcgtcagggg cagtggcgaa tatcacggcg tcgcgcgtcg gcacggagaa
     1801 gattcggaag atgcgtttct tccagccgca cgattacatc gcgatcgatt acacgacgaa
     1861 gcgcgcgtcg gtcagtagtc tcgcgccgcc gggagcgagc ggcgcgtggc ctggcgttca
     1921 cgtgaagcat ctcgacatca ttgacgtcga gccgttgcgc gcggagattg tttcgtttct
     1981 ggaggcagcg cgtgatggcg cgccgcccgc ggtctccggg atcgagggaa gaaatgcgtt
     2041 gagtttggcg ctgcgcactc tcgagagaat ccgcgagcat gcggagcatc cgggagtgaa
     2101 gccacagata acacggggtt gaaatgccgg ttcggaacga tcaactttcg gcgctcctgc
     2161 aacagcacat cgaggcgggc gagtttccgt cggctgttta tctcgtcggc gagcgcgatc
     2221 aggtcatcta cgaggatgcc ctcggcaatt ccgtcgtcga accttatcgc gtcgcaaaca
     2281 agatcgacac gatctacgat ctcgcatcac ttacgaagcc gttggtcacc gcgttgttat
     2341 gtgcgcgtcg catcgagctc ggcgagttaa cactcgacag ctcggtttct cactacctgc
     2401 cggagttcga tcgtaccgac aagcagatga tcacgattcg tgagctgctg acgcacactt
     2461 caggtctgcc ggcgtggcga ccgttgtacg tgctcaccga ggagcagccc gatcgtgcgg
     2521 cgggtgcgat cgcgagtctc gatctcgagt acaagccggg cacgcgtgtg gtttacagcg
     2581 acctgggatt tatcgcgttg ggaattttgt tggagcgcat gatgcatcag catctcgcag
     2641 acatggcgaa gcatgagatc ttcgagccgc tgaagctgca acagacgttt ttcaatccgg
     2701 agatcgcttt gcaaaccggg attgcggcgt gcgagaccgg caacgcatac gagctcgata
     2761 tgtctaaaca aatgggcgcg ggtgagtatg cgaattcgcg gcagcgattg atctggggcg
     2821 aagtacatga tggtaatgct tactttttag gcggcgcggc gggacatgcg ggattgttct
     2881 cgactgcgcg cgaaacgttt cagatgacgc agcagttttt gagtgagtcg acgacgctgc
     2941 tcgcgccgga gacgtgcaga ctgtttcgag agaacatgac gccgggtttg gaagaggcgc
     3001 gctcgatcgg atggcaactg gcggcgacga aggattcaac gggcggaacc gatctgccgc
     3061 ctgacagctt cggtcacaac gggtttacag ggacgtgtgt ctggatcgat ccgagccggc
     3121 gacgcgtctt cattctcctc acgaaccgca cccatgcgcg cgcgctgccg tttgcgaata
     3181 tcaatgcagt gcggcggcaa ttcaatacat tagccgcgga tattacggat aaaaaagcgg
     3241 atttatatct tgagccgtaa ggtggttccg atcacattta agtcgccggg aatgctcacg
     3301 ccgtcgttct ggcgaagata atagatatcc tgagtaaagt gtttattgaa gaccttgctg
     3361 ccgccgacgg tgaatcgatt gcgcacccag cgatcgatgc tccagtcgta gaagacttca
     3421 tccgcgacat acaacgatag tccccacttc gatggtccaa ccggatgctc gacctgcaag
     3481 cgattacgat atcgcgtcac attaattccc ggacgtcgaa agcgtcgctc aaa
//
LOCUS       DASSFQ010000119        15453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_643044, whole genome shotgun sequence.
ACCESSION   DASSFQ010000119 DASSFQ010000000
VERSION     DASSFQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15453
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643044"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VFH96_03965"
     CDS             complement(<1..1369)
                     /locus_tag="VFH96_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HET6973154.1"
                     /translation="MTAQTELDPKLRDTAFIGHPKGLGPLFFTELWERFSYYGMRAIL
                     VLYMVAPPAQGGLGFDVKRAASIYGWYTMSVYITALPGGLAADYLLGARLAVFLGGLV
                     IAAGHFSMVFQSMTSFYLGMVLIAIGTGLLKPNISAMVGGLYGENDPRRDSGFSIFYM
                     GINIGAVLAPLVCGYLAQSKSFQQTLANNGFDPNTSWHWGFGAAGVGMLLGLVIFLLF
                     GSWLSHVGSRKKKKSVEKASDAAANAPLTSSDWKRIAAIVIFFLFTILFWGAYEQKGA
                     SLNLFAKELVRTEVFGVAFPSSYLQSCTPFFVIILVPVFATLWVKLGNRQPSSPVKFT
                     LGLLFIGAAYLLMIPACMLTASGRISPLWLVGLYFLEVCGEMCLSPVGLSTVTKLAPV
                     KLVGIMMGVWFLASSFGSKLAGYLSGFFVANNSWQLVKLYGGIAAGLLIAAVILAFLT
                     PTVKRL"
     gene            1725..2855
                     /locus_tag="VFH96_03970"
     CDS             1725..2855
                     /locus_tag="VFH96_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973155.1"
                     /translation="MYKTGLALILLLSVSFGLYAQSGRKMEPKPKPEPTKSADGELNE
                     SKVSPGGETVEGDVIRFDTALVMVPVSVLDRNGRYVPLLRKDQFRLAENGVDQKIAYF
                     AATDSPFSVVLLIDTSGSTQMRLDDIHDAAMKFVEKLKPTDSVMVMSFDDRIEVQCKA
                     TTDRDVIEKAIRRTRTGGGTRLYDAVDDILRKQLQTIPGRKAVVLFTDGVDTTSHRAS
                     YDSTIRLAEESDAPIYSVDYDTSGMGGIMGPGTGGRRGGVIFGIPFPTPGIPGSTIPG
                     SSPGDYRRAVAYLHALSDQTGGRFYSGDSMFGIGQAFTWIAEELGRQYSLGYYPSTPG
                     KEGERRQIKVRVTESDMVVKARDSYIYSDKSKKFSQVTRLVN"
     gene            3387..3959
                     /locus_tag="VFH96_03975"
     CDS             3387..3959
                     /locus_tag="VFH96_03975"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6973156.1"
                     /translation="MSEHPSDNVTRLLIELSNGDRAAVDLLLPVIYDELRKLAANYLR
                     RERPDHTLQPTALVHEAYLRLIDQSRVNWQNRAHFFGVAAQIMRRLLVDHARKHNAEK
                     RGQDFQKLSLDENIDRAVERSAELIALDDALKTLATFDANKARMVELRYFGGLSIEET
                     ADVMGVTPTTIKRHWRLAKAWLHGEMQRNS"
     gene            4080..9215
                     /locus_tag="VFH96_03980"
     CDS             4080..9215
                     /locus_tag="VFH96_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6973157.1"
                     /translation="MTPERWRQVEEIFQAALDLSPEDRDRYVLDVCKEDTELKRDVES
                     LLSQYDSAGELLEEPVYGNTEMSVLESFIEEKDPMLGRRLGSYRIEREIGRGGMGAVY
                     EASRADNEFNKRAAIKLVKRGMDTDFVLRRFRKERQILAALDHPHIAGLLDGGTTEDG
                     LPYFVMEFIEGQPLYSYCDNNKLNITERLKLFRSICDAVHYAHGKQVVHRDIKPSNVL
                     VTSEGTAKLLDFGIAKLLNPQLAGDITHDPTATAMRLMTPEYASPEQVQGAPTTPTTD
                     VYSLGVLLYELLTGHRPYRLVNRAPHEIARVICEEAPAPPSIIITRTEDLLPSFNAGD
                     DSGTLKRLYTARGTTLESLRRALSGDLDNIVMQALRKEPEWRYQTAADLRDDITRYLE
                     GRPILDLPDPPHGSGSLRPDTTENSLAVLPMKILDINQPDTGPDYLGTGLADALITRL
                     SAIRSFAVRPTSSVLRYGADADPLRAGRELGVEFVLDGRIRRTAERIRVTIQLLNVRD
                     ETTAWAGQFDEQFTDVLNLEDVISSHVAEAIVPHLTVDQRLRLAKRGTDNPQAHEAYL
                     RGRFYWNTFTEEGFARAIVCFQQAIALDPKYALAHAGVANYHNWLGVFSVMPFAECSA
                     AAYEAASTAVEIDPNLAEGHVALGQAILCRDFAWSSAEHHLVRAVELDPNYAPARVWY
                     SLQLAMEGRFSEALREAHTGRDLDPLAVISRFAVIWCSYHARRFDEAYRFATATLENE
                     PRNLMALYGSSFVLSRLSRHAEAVEAAERCVELMGKASHTLGRLGSAYAAAGDLEKAQ
                     SVLNDMHLLAERRHVSPYHLALVTCQLDRPEEALDLLERAYESGDAKVLWSGVDPELD
                     PLHGHPRFNDLLRKLDHRLAALPTIAGQLREDQESIAVLPFRILSPPGENTGDEYLGI
                     GLTDALITRLSNVRRLVVRPTSSVLRYHGAAIDPLMAGRDLGIDYVIDGSLRRVGNRI
                     RVTAQLLSISDGQTRWAEQFDEDSVDVLQIEDSISEKVAAALLPQLTGDEKRQLSKRG
                     TDSSEAFEAYLRGRYYWSSYTEVGLAKALESFKKAIKLDPDYALAYTGIADYYNWLGV
                     FGIRPFAETSAAAKQNAAKAVELDSTSAEAYSALGFATVCHDFDWAVAEGQHRRAIEI
                     NHNYATAHHWYAFHLLMEGRFEEAIERILRTRELDPVSPSVLQAVAWCYYQARRFDES
                     IATYKSMLEAVPEFPYGLVTQSWTLRHTGDFDEAVKAAEKALDLSGGGTFYITALGAA
                     YAAAGREKDARAALERLAQLSVHGYVSPYQLSLIHLHLGERERALELLQDAYAIRDAW
                     VVWLGVEPQFDPLRGDPIFEGILRDLRHPALYRRIEKQTAAAAREKRRRPGSHIAPIT
                     TAILMPAPDTQTGENEEARQLYTAGRYYSTRRTAEGLRQAIERFERAVEIDPEFAIAH
                     AELADCYALMNWYVEPPPAGAWERAKESALRAVEADPNLPEAHASLGFVKLHYDRDWI
                     DAERELRKAIQLKPSNQVAHRWYAYSLSAMGRHDEAYAEIERARQISPQSAVIATAVA
                     NVLFLAGKFDEAVAQARKSLETDPGAVAAHTILRWAYELKGMHNEAMAAFEQERSFAG
                     DTPTTHAKRAHVLAAIGKKDEARQILNEIIAKRQQNWVTAYEIAIIYTWLGDLDNAFR
                     WLAQAEREHAVGFTFVRVDPHLQKLRLDPRFADLMRETEKTIP"
     gene            9252..10283
                     /locus_tag="VFH96_03985"
     CDS             9252..10283
                     /locus_tag="VFH96_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973158.1"
                     /translation="MAKKPDELDAWVREVVAWHFDPETGCPFWLDYAKNLSWDPRKEI
                     QTYADLSRFGFFQDEWLRGGPVRRWVPKGYANRPVYTFETGGSTGVPKSRINIDDFRI
                     DYEAFSDTLPEEFFPRGADWLSLGPTGPRRLRLAVEHLAQHRGGICFLVDLDPRWVIK
                     LIKMGQPKMAEQYKQHVVEQGLTLLKAHDGIRCLFTTPKLLEALCEKVSLKKMGITGV
                     FCGGTEMTPQFHRFAVEELLEGAYFAPTYGNTLMGLAVHKTRDPKDNYAIIYYPPLPR
                     AAVEVVDPDQPDRIVGYGETGRVRLTTLTKEFFMPRFLERDEAEREPPCDEYPWDGVR
                     NVRPFSRFQ"
     gene            10345..11769
                     /locus_tag="VFH96_03990"
     CDS             10345..11769
                     /locus_tag="VFH96_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6973159.1"
                     /translation="MIHLPILRHGNQYKSLDISRVPHHQTRELFVEVSQANAGLIRRD
                     LSKQNIATDALSHFSTRDLVDICARAGEIFTNDSLPLGDSTQSSEDYVHQVSATTGLP
                     YVLARRNMQKIKSMFANMESVLNGLTRNLDWELLDRGFGELDGHALSFFPRTQSLGAV
                     LPNNSPGVHSLWIPAFPLRIPLVLKPGSAEPWTPYRIIQSLIKAGAPPEAFSFYPTDH
                     AGAAEILRSCGRSLLFGDSSTTGVWANDPRVEIHGPGYSKIVIGADCVDDWEQYLDII
                     IASIADNGGRSCVNASAVWTPAHAEEISEALAKRLAQIVPRAADDENAQLAPFVDPQV
                     AARINAIIEQGLTEPGARDVTAAYRDSERLVQWNNCSYLLPTVILSEHDHPLAMKEFL
                     FPFASVLKVDQNDLSSLLGPTLVVTAITKDPKLIQKLVASSRIDRLNIGALPTNQISW
                     DQPHEGNLFDHLYARRAFQQAVAV"
     gene            11815..13095
                     /locus_tag="VFH96_03995"
     CDS             11815..13095
                     /locus_tag="VFH96_03995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973160.1"
                     /translation="MYCGSCLRDNAMATELMKRGHDVLLLPVYTPTLTDEPNVSTDHV
                     VLGGISAYLEQYVPLFRKTPRWLDRLWDSKTALNLATRRSLSTNPKMLGEMTVSVLKG
                     EGGFQRKEIDKFIDWVKDEAPPDVVSLPYTLLLGLAQPIKHALNVPILCTLQGEDLFL
                     DNLQEPYRTQSLDLIRDHLLHVDLFLSVSDYYADFMPGYLGIPREKIRVVPIGINPEG
                     FELRHADRSGPFTVGFFGRIAPEKGLHVLAEAYCIARQSGELPEARFEAAGYMAPDSK
                     PYLEAIQKRLHDAGLGNEFHYRGVLERADKIAFLRGLDLMSMPATYDEPKGVSLLEAM
                     ACGVPLVQPRRGSFTEIIERTGGGLLVQPDDPQSLADGLLQIAGDQNLAADLSANGFR
                     GVREHYTAVHMADRVMECYNEAVANPSSSVLIRG"
     gene            13099..13446
                     /locus_tag="VFH96_04000"
     CDS             13099..13446
                     /locus_tag="VFH96_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973161.1"
                     /translation="MFVPGDDLIFQLESAFGLLRVLAVEGEGAETVWHLLAYDEFFPD
                     VESAEQRLAAGVPLPVRQAHMALTNRAFERTPTARLGNRPVTDDELVAYNTWLNSDRT
                     VSDRSALLMLGIR"
     gene            13449..15089
                     /locus_tag="VFH96_04005"
     CDS             13449..15089
                     /locus_tag="VFH96_04005"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET6973162.1"
                     /translation="MPSSLRPLRVCVFALLILFPFTTFASRIERLIDTWRPTHYLVNI
                     TLNDQLSEIASANARIYLTIVKPTREIDIDFGDLTVDRVTLNGSSSTFLRRDGKLHFD
                     LPQAASAGDVYIIEVYYHGKPKDGLILTNDKDGKPAAIGDNWPDRVHHWIPVLDHPSA
                     KATVTFNVTAPAGQEVVANGRLDHVETTASGQRTWTYSEGVPIPPYCMIIGVGQFARA
                     ESAQRALTPLSYYVPLSERELAQKGFSPAIPTIEFFTEKVAPYPYEKLALIVGATRYG
                     GMENSGAIVFTSSLFNRNSTVGMSKTYGIPFGNVTLIAHEIAHQWFGDSVTESTWSDL
                     WLSEGFATYFAGLFVQRYESEEAFQRYMRDASASVFAYEKKKRVPIFDRDTENLMELL
                     NPNNYQKGAWVLHMLRSNLGDDAFFRGIRAYYESHKNSVASTEDLRAALEKSSGKDLR
                     TFFARWVYDTGHPQYELKWFWLGKKELRVVLTQKQPGNVFVDPIHLTISTAKGKRDVV
                     LKPTGKLFIERIPLRDSPIKLDLDPNNQLLDESTIAPG"
     gene            15136..15212
                     /locus_tag="VFH96_04010"
     tRNA            15136..15212
                     /locus_tag="VFH96_04010"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15170..15172,aa:Pro,seq:cgg)
     gene            complement(15219..>15453)
                     /locus_tag="VFH96_04015"
     CDS             complement(15219..>15453)
                     /locus_tag="VFH96_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973163.1"
                     /translation="LGDRGASAFEIAQIMGWSDIRMAMRYTHATGDGIRRAMQLLTQT
                     GVGNSKVVQLQNWTDTKVPTSNGNGQHALPVSA"
BASE COUNT         3419 a         4597 c         4211 g         3226 t
ORIGIN      
        1 tcaagcgttt tacggttgga gtcaggaatg ccaggatcac cgccgcgatc aataatcccg
       61 ccgcaatacc tccgtacaac ttcacgagct gccacgaatt atttgccacg aagaaccctg
      121 acaagtaacc cgccagcttg cttccaaacg acgacgccag aaaccaaacg cccatcatga
      181 tcccgacgag cttcacgggc gcgagtttcg taacggtact cagtcccacc gggctcaaac
      241 acatctcgcc acaaacctca aggaagtaaa gaccgaccag ccacagcgga ctgatccttc
      301 cgctcgccgt caacatacac gctggaatca tcagcagata agccgccccg atgaacaaca
      361 gcccaagcgt aaacttgacc ggactcgatg gttgccggtt gcccagcttc acccacaaag
      421 ttgcgaacac aggcacaagg atgatcacaa agaacggcgt acacgattgc aagtagctcg
      481 acgggaatgc gacgccgaaa acttccgtac gcaccagttc tttcgcaaac aggttgagac
      541 tcgcgccctt ctgctcgtac gcgccccaga acaggatcgt aaatagaaag aagatcacga
      601 tcgctgcgat gcgtttccag tcagacgatg tcaacggcgc attcgccgcg gcgtcgctcg
      661 ctttctcgac agattttttc tttttccggc taccgacgtg cgagagccag cttccgaaaa
      721 gcaaaaagat cacaaggccg aggagcatgc cgacgcccgc cgcaccaaat ccccagtgcc
      781 agctcgtgtt gggatcgaag ccgttgttgg cgagagtttg ttggaagctt ttactctggg
      841 cgagatagcc acacacgagt ggcgccagca ccgcgccgat gttgataccc atgtaaaaga
      901 tcgagaaccc gctatcgcga cgcggatcgt tttcgccgta aagaccgccg accatcgcgc
      961 tgatgtttgg tttcaaaagc ccggtaccga tggcgatgag taccatgccg agatagaagg
     1021 acgtcatcga ctgaaagacc atcgagaagt ggcccgcggc gatgacgagc ccgccgagaa
     1081 agacggccag acgcgcgccg aggaggtagt cagcggcgag tccacccggt aacgcggtta
     1141 tataaacgga catcgtgtac caaccgtaga tcgaggccgc gcgctttacg tcaaaaccca
     1201 aaccgccctg cgctggaggc gcaaccatat aaaggacaag gatggcgcgc attccgtagt
     1261 aactgaagcg ctcccaaagc tcggtgaaga agagcgggcc gaggcctttc gggtgtccga
     1321 tgaaggccgt gtcgcggagt tttgggtcga gttcagtttg tgcggtcata attgcgcggc
     1381 aatatagttg agagtagcag ttacattcaa ctaaataagc ccgccatagc ggcaattgat
     1441 tttagcccgc gatagcgggc gcgagcataa agcctggggc gcaagcccca ggatcacaga
     1501 ataaaaagat gattgagccc gtgaaaatgg gcgacagcct ttgcctgttt cgtctgtcgc
     1561 ccgcttcgcg ggctctgacc ctcatttttt tccctttcct ggggcttgcg ccccaggctt
     1621 tatgctttcg cccgcttcgc gggctttcca acccttaagc ggtcagtcgc atctatccgc
     1681 tacaccactc tcccgtacgc gctcgctcca aaccaggaat tcgtatgtat aaaactggac
     1741 tcgcgctgat tctccttttg agtgtctctt tcgggctcta cgcacagtca ggccggaaga
     1801 tggaaccgaa accaaagcct gaacccacca agtccgcgga cggagaacta aacgaatcca
     1861 aggtcagccc cggcggcgag acggtcgaag gtgatgtgat tcgcttcgat accgccttag
     1921 tcatggttcc cgttagcgtt ctggatcgaa acggccgcta cgttcccctt ctccgcaagg
     1981 atcagtttcg actcgcggaa aacggcgtcg accagaaaat cgcttatttc gccgccaccg
     2041 attcaccatt cagcgtcgtc ctgctgatcg acaccagcgg ctcaacgcaa atgcgtctcg
     2101 acgatatcca cgacgcagcg atgaagttcg tcgagaaact gaaaccaaca gacagcgtga
     2161 tggtgatgtc gtttgacgac cgcatcgagg tgcaatgcaa agcgaccacc gatcgagacg
     2221 tgatcgagaa ggcgatccgt cgcacacgca ccggcggcgg cacccgcctc tacgatgccg
     2281 tcgacgacat tctccggaaa cagctccaga cgatccccgg tcgcaaggca gtcgtcctgt
     2341 ttactgacgg cgtcgacacg acgagccatc gcgcttcgta cgacagcacg atccgtctcg
     2401 ctgaagaatc agacgctcct atttattctg ttgactacga tacgtccgga atgggcggca
     2461 tcatgggtcc gggcaccggc ggccggcgcg gcggcgtgat tttcggcatc cccttcccga
     2521 ctcctggcat tcccggctca acaatccccg gctcatcacc cggcgattac cggcgcgccg
     2581 tcgcttacct gcacgcgctc tcagaccaga ccggcggtcg tttctacagc ggcgattcaa
     2641 tgtttggaat cgggcaggcc tttacgtgga tcgctgaaga gcttggacgg cagtacagcc
     2701 tcggctatta cccgtcgaca ccggggaaag agggggaaag aaggcagata aaggtgaggg
     2761 ttactgaatc tgatatggtc gttaaggccc gcgatagcta catctactct gacaagagca
     2821 aaaagttcag ccaggtaacg cgactcgtta attagtgtaa aaaaacttac cctgcatttc
     2881 ccagttgccc agattaaact ggcgctgcgc accacagctc aagcatttcc gatacgcgcg
     2941 ccccccgcgc gaaaacggac ggctcagctc cccgtgccag caaccaaaaa cccgtgtaac
     3001 aaaactgcta atttttcccg ccacagcctg catgatgttt acctctcgaa atctcccaaa
     3061 ttcctgttca cccactaacg cgcaaacaat ccgcgtttcg ggtcatagtt gtcgctccac
     3121 tgcggtgcaa aacgccgaag gcgttggcta atttgagccc agggttcgag gaacgagaaa
     3181 ccctgggtta gcaatatgaa tagagcacaa aaccctgaaa gggttccgag attggcgaac
     3241 cctttcaggg ttcaaaattt ttttgtttcg taacccaggg ctcaaattgg cgaacgcctt
     3301 cggcgttatc attcagccct accgcaagag gttctttcgc tttatactaa tggaccttta
     3361 agtcttcgat tatggagagt taacgattga gtgagcaccc ctcagacaac gtaacccgtc
     3421 tactgatcga gctctctaac ggcgatcggg ccgcagttga tcttttgcta cccgtcatct
     3481 acgatgaact gcgtaagctg gcagcgaatt atctgcgacg ggagcgtccc gatcacacac
     3541 tccagccgac cgcgctcgta catgaggcgt atctgcgact catagaccag tcgcgggtga
     3601 actggcaaaa ccgcgctcat tttttcggag ttgccgcaca gataatgcgt cgcctgctcg
     3661 tcgatcatgc gcgcaagcac aacgcggaaa agcgtggcca ggattttcaa aaactctctc
     3721 tggacgaaaa catcgatcga gcagttgaac gaagcgcgga gctgatcgcg ctggacgacg
     3781 cgctcaaaac actggcgaca ttcgacgcaa acaaggcacg catggttgaa ttacgttact
     3841 tcggcgggct ctccatcgaa gagacagcag acgtgatggg tgttacccct acaactatca
     3901 aacgtcactg gcgcttagcc aaagcctggc ttcatggaga aatgcaaaga aatagttgat
     3961 gcacgcatta agtccaatcc agcaaggcaa ctagaacatt ctttgtgcaa tctgtgttct
     4021 ctgtgttctg tgattaagcc agacacagag agcacggcga ttgcacagta aagacccgaa
     4081 tgacccccga acgctggcga caagttgaag aaatcttcca ggctgcgctc gatctttctc
     4141 ccgaagatcg agaccgctac gtgttagacg tgtgcaagga agacaccgag ctcaaacgcg
     4201 acgttgaatc gttgctttcg cagtacgaca gcgccggaga acttttggaa gagcccgtgt
     4261 acgggaacac tgagatgagc gtcctcgagt cgttcatcga agagaaagac ccgatgctcg
     4321 ggcggcgtct gggttcctat cgcatcgagc gtgaaatcgg acgcggcggc atgggcgccg
     4381 tctacgaagc ctcacgcgcc gataacgagt tcaacaagcg cgcggccatc aaactcgtca
     4441 aacgcggcat ggacacagac ttcgtcctgc gccgcttccg caaagaacga cagatactcg
     4501 ccgccctgga ccacccacac atcgccggat tgcttgacgg tggcacgacc gaggatggtc
     4561 tgccctactt cgtgatggag tttatcgagg ggcagccgct ttacagttat tgcgacaaca
     4621 acaaactgaa catcaccgag cgactgaaac tttttcgctc catctgtgat gcagttcatt
     4681 acgcacacgg gaaacaggtc gtgcaccgcg acatcaaacc gtcgaacgtc ctcgtgactt
     4741 cggagggcac cgcgaaactt ctcgacttcg gtatcgccaa gttgctcaat ccacaactcg
     4801 ccggcgacat cactcacgat ccgacggcga ctgcgatgcg tttgatgaca cctgagtacg
     4861 cgtcgccgga acaagtgcag ggcgcaccga cgacaccaac caccgacgtc tattcactcg
     4921 gcgtgttgct gtacgaattg ttaaccggtc accgtccgta tcgtctcgtt aatcgcgcgc
     4981 cgcacgagat cgcgcgcgtg atctgtgaag aagcacccgc accgccgagc atcatcatca
     5041 cgcgcaccga ggatctgttg ccgagtttca atgccggcga cgattcaggc acgctcaagc
     5101 gtctctacac cgcgcgtggc accacactcg aatcgctgcg ccgcgcgctc tcgggcgatc
     5161 tcgacaacat cgtgatgcag gcgctgcgca aggaacccga gtggcgctat caaaccgcgg
     5221 cagacctgcg cgacgacatc acgcgctatc tcgaaggtcg tccgattctc gacttgcctg
     5281 atccaccgca cggcagcggc tccctccggc ctgacacgac ggaaaactct ctggccgtgt
     5341 tgccgatgaa gattctcgat atcaaccagc ctgacactgg tccggactat ctcggcacag
     5401 gcttagccga cgcactcatc acgcgtctca gcgcgattcg cagttttgca gttagaccaa
     5461 cgagcagtgt gctgcgttac ggtgctgacg ccgatccgtt gcgcgcgggc cgcgagcttg
     5521 gcgtcgagtt tgttctggac ggcagaatca ggcgcacggc ggaacgcatt cgcgtcacga
     5581 ttcaactgct caacgttcgt gacgagacga ccgcgtgggc cggacagttt gacgagcagt
     5641 ttacagacgt tctcaacctc gaagacgtca tctcttcgca cgtcgccgaa gcgatcgtgc
     5701 cgcacctgac ggtggaccag cgcctgcgac tggccaagcg cggcaccgac aatccgcaag
     5761 cgcacgaagc ttacctgcgc ggacgttttt actggaacac gtttaccgag gagggtttcg
     5821 cgcgcgcgat cgtctgtttc cagcaggcca tcgcgctcga tccgaaatac gcgctcgcgc
     5881 atgccggtgt cgccaactat cacaactggc tcggcgtgtt cagcgtaatg ccgtttgctg
     5941 agtgttcggc agcggcttac gaggcagcgt caaccgcagt tgagatcgat ccgaatctcg
     6001 cggaaggtca cgttgcgctc ggacaggcga tcctttgtcg cgacttcgca tggagcagtg
     6061 ccgaacatca tctcgtacgt gcggtcgagc tcgatccgaa ttacgcgccg gcgcgtgtct
     6121 ggtattcgtt gcagctcgcg atggagggac ggtttagtga agcgctccgc gaagcgcaca
     6181 ccggtcgcga tctggatcca ctcgcagtca tctctcgctt tgcagtgatc tggtgttcgt
     6241 atcatgcgcg gcgttttgac gaggcttatc gttttgccac tgcgacactc gaaaacgagc
     6301 cgcgaaatct gatggcgcta tacggctcga gtttcgtgct gagtcgtttg agccgtcatg
     6361 ctgaggcggt tgaggcggct gagcggtgcg ttgagttgat gggcaaagct tcgcacacac
     6421 tgggacggct cggtagtgct tatgcagctg ccggagatct cgagaaggcg cagtcagttc
     6481 ttaatgatat gcatttgctg gctgagcgtc gtcacgtttc gccgtatcat ctcgcactcg
     6541 taacctgtca gctcgatcgt ccggaagaag cgctggatct actcgagcgg gcttacgagt
     6601 caggcgacgc aaaagtgctt tggtcgggag tcgatcccga gctcgatccg cttcacggtc
     6661 atccgcgttt caacgatctg ttgcgcaagt tggaccaccg tctggcggcg ctcccgacga
     6721 ttgccgggca gttgcgcgag gaccaggaat cgatcgccgt acttcccttc cgcattctga
     6781 gtccgccggg agaaaacacc ggtgacgagt atctcggtat cggacttact gacgcactga
     6841 tcacgcgact cagcaacgtt cggcgtctcg tcgtgcgacc gacgagcagc gtgctccgtt
     6901 atcacggcgc cgccatcgat cctttgatgg ccggacgcga tctcggtatc gattacgtga
     6961 tcgacggcag tctgcgtcgc gtcggtaatc gcattcgcgt gacggctcaa ctgctgagca
     7021 tcagcgacgg tcaaacacgt tgggccgaac agtttgacga agattcagtc gacgttcttc
     7081 agatcgagga ttcgatttcc gagaaagtcg cggcagcgtt actaccgcag ctcacgggcg
     7141 atgagaagcg ccagttatca aaacgcggca ctgacagctc cgaagctttc gaggcttacc
     7201 tgcgcggccg ttattactgg agctcttaca cggaagtcgg tctggccaaa gcgctcgaga
     7261 gtttcaaaaa agccatcaag ctcgatccgg attacgcgct cgcatacaca ggcatcgccg
     7321 attactacaa ctggctcggc gtgttcggca ttcgcccgtt cgcagaaacg tcagccgccg
     7381 cgaaacaaaa cgctgcgaaa gccgtcgagc tcgattcaac ttccgccgaa gcatacagcg
     7441 cgctcggctt cgcgaccgtt tgtcacgact ttgattgggc cgtcgctgaa ggccaacacc
     7501 gccgcgcgat cgagatcaat cacaactacg ccaccgctca tcactggtac gctttccatt
     7561 tgctcatgga aggacgcttc gaagaagcga tcgagcgaat cttgcgcaca cgcgagctcg
     7621 atccggtttc gccgagtgtg ctgcaggcgg ttgcgtggtg ttactaccag gcgcgtcgct
     7681 ttgacgagtc gatcgcgact tacaagagca tgctcgaggc ggtgccggag tttccctacg
     7741 gactggttac gcagtcatgg acattgcggc acacgggcga cttcgatgag gcggtgaaag
     7801 cagccgagaa ggctctggat ctttcaggcg gcggcacgtt ttacatcacc gcgctcggcg
     7861 ccgcgtatgc agccgccggc agagagaaag atgcgcgcgc agcgttggag cgcctcgccc
     7921 aactctcagt gcacggctac gtttcgcctt atcaactgtc gttgatccac ctgcatctcg
     7981 gcgaacgcga gcgcgccctc gagttgctac aggatgctta cgcgatcaga gacgcgtggg
     8041 tcgtgtggtt aggtgtcgag ccgcagtttg atccgttgcg tggcgatccg atcttcgaag
     8101 gaattcttcg cgacttgcga catccggctt tgtatcgacg aatcgaaaag caaactgcag
     8161 ccgcagccag ggagaagcga cgaaggcccg gctcgcacat cgcgccgatc acgaccgcga
     8221 tcctgatgcc cgcgcctgac acgcagacgg gggagaacga agaagcgcgc cagctctaca
     8281 ccgccggacg ttactattcg acgcgccgaa cagcggaagg cttgcgacag gcgatcgagc
     8341 gtttcgagcg tgccgtcgag atcgatcccg agtttgcgat cgcgcacgcg gagcttgccg
     8401 attgctacgc gttgatgaac tggtacgtcg agccgccgcc ggcgggcgcg tgggagcgcg
     8461 cgaaagagtc cgccctgcgt gcggtcgagg ccgatccgaa cctgcctgag gcccatgcgt
     8521 cactcggatt tgtgaaactt cactacgatc gtgattggat cgacgctgaa cgcgagctac
     8581 gcaaagcgat tcaactgaaa cccagcaacc aggtggccca tcgctggtac gcttacagtc
     8641 tgtcagccat gggacgtcac gatgaggctt acgcagagat cgagcgcgcg cggcaaatct
     8701 cgccgcaatc ggcagtgatc gccacggccg tcgcgaacgt actgtttctg gcaggcaagt
     8761 ttgacgaagc agtggcccag gcgcgcaagt cgctggagac cgatccgggc gcagttgccg
     8821 cacatacgat tcttcgttgg gcgtacgagt tgaaaggcat gcacaacgaa gcgatggctg
     8881 cgttcgaaca ggagcgaagc ttcgcgggcg acacgcccac tacgcatgcg aaacgcgcgc
     8941 acgtgctcgc cgcgatcggc aaaaaggatg aagcacggca gatcctgaac gagatcatcg
     9001 cgaagcgtca gcagaactgg gtgacggcgt atgaaatcgc aatcatctac acgtggcttg
     9061 gcgatctcga caacgcgttt cgctggctcg cacaggctga acgcgagcat gcggttggtt
     9121 tcacgttcgt gcgcgtcgat ccgcacttgc aaaagcttcg tctcgatcca cgtttcgctg
     9181 acttaatgag agaaaccgag aaaaccatac cttaatccgt ataaatcgtg actaacaaat
     9241 ccgtatcatc cgtggctaaa aaacccgacg agctggacgc ctgggttcgt gaagttgtcg
     9301 catggcattt cgatccggaa acgggctgtc ctttctggtt agactacgcc aaaaatctct
     9361 cgtgggaccc gcgtaaagaa attcaaactt acgccgacct ttcgcgtttc ggatttttcc
     9421 aggatgagtg gctgcgcggt ggtccagtgc gacgctgggt accgaaaggt tacgccaatc
     9481 ggcctgtcta cacgttcgaa accggcggca gcacgggcgt gccgaagtcg cgtattaata
     9541 ttgacgactt tcgtatcgac tacgaagcgt tcagtgacac gctgcccgaa gagtttttcc
     9601 cgcgcggcgc tgactggctg tcgcttgggc caaccggtcc gcgtcgtctg cgtttggcag
     9661 tcgagcatct ggcgcagcat cgcggcggga tttgtttcct cgtcgatctc gatccacgct
     9721 gggtcatcaa gttgatcaag atgggccagc cgaagatggc tgagcaatac aagcagcacg
     9781 tggtcgagca aggtcttact ttactgaaag cgcacgacgg tattcgttgt ttgtttacga
     9841 cgccgaagct gctcgaagcg ctgtgtgaaa aagtgtcgct gaagaagatg gggatcaccg
     9901 gcgtgttttg tggcggcacg gagatgacgc cgcagtttca tcgcttcgca gttgaggagt
     9961 tgctcgaggg tgcgtacttc gcgccgacat atggcaacac gttgatgggc ctggccgttc
    10021 acaaaactcg cgatccgaaa gataactacg cgatcattta ctatccgccg ttgccgcgtg
    10081 cggcggtgga agttgtcgat ccggatcagc ctgatcgaat tgtgggatat ggcgagaccg
    10141 gccgcgttcg cttaacaact ctgacgaagg agttcttcat gccgcggttt ttggaacgcg
    10201 acgaagccga gcgtgagcca ccttgtgacg agtatccgtg ggatggcgtg cgcaacgtcc
    10261 gtcccttctc gcgatttcaa tgaaccgtag tcgaaggtgt ttattaaaat cttgtacatc
    10321 ccgtaaatcc tgaaaatcct gtctatgatt caccttccca tcctgcgcca cggcaatcaa
    10381 tacaagagtc tcgacatctc gcgcgtcccg catcatcaaa cacgcgagct cttcgtcgaa
    10441 gtaagccagg ccaacgccgg actgatccgc cgcgacctct caaaacagaa catcgccacc
    10501 gacgctctct cccacttctc aacccgcgac ctcgtcgaca tctgcgcacg cgcaggtgaa
    10561 atttttacga acgattcgct cccccttggc gattcaaccc aatcatctga agactacgta
    10621 catcaagtct ccgcgacgac tggtctccca tacgttctcg cgcgccgcaa catgcaaaaa
    10681 atcaaaagca tgtttgcaaa catggaatcc gtgctcaacg gtctcacgcg caacctcgat
    10741 tgggaactac tcgatcgcgg cttcggcgaa ctcgacggcc acgcgctcag tttcttcccc
    10801 cgcacgcaat cactcggcgc agttctacca aacaattcgc ccggcgtgca ctcactctgg
    10861 atccccgcgt ttccactacg gattccactc gtgctcaaac ccggcagcgc tgaaccatgg
    10921 acgccttatc gaatcatcca gtcgttaatt aaagccggcg cgccacccga agctttcagt
    10981 ttctatccga ccgatcacgc cggcgccgca gagatcctgc gaagctgtgg ccgttcgctt
    11041 cttttcggcg actcatcaac caccggcgtg tgggccaacg atccacgtgt tgagatacac
    11101 ggcccgggct acagcaagat cgtcatcggc gcagactgcg ttgacgactg ggaacagtat
    11161 ctcgacatca tcatcgcctc gatcgctgac aacggcggcc gctcgtgcgt gaacgcttca
    11221 gcagtctgga cacctgcgca cgcggaagag atcagcgaag ccctcgccaa acgtctggcg
    11281 cagatcgtcc cacgggctgc tgatgatgaa aatgcgcagc ttgcaccatt cgtcgatccg
    11341 caagtcgccg cccgcatcaa cgcgatcatc gagcagggtt tgaccgagcc cggcgcacgt
    11401 gacgtcacgg ccgcatatcg cgatagcgaa cgcctcgtcc agtggaacaa ctgctcttat
    11461 ctgttgccaa cagtgattct ctccgaacac gatcatccgc tcgcgatgaa agagtttctc
    11521 tttccgttcg ccagcgtcct aaaagtcgat caaaacgacc tctcgtcact tcttggtcca
    11581 accctcgtcg tgactgcaat cactaaagat ccgaaactga ttcaaaagct cgtcgcctca
    11641 tcgcgtatcg atcggctcaa tatcggcgcg ctcccgacca atcagatcag ttgggaccag
    11701 ccgcacgaag gcaatctctt cgatcatctt tacgcgcgtc gcgcgtttca gcaggcggtc
    11761 gccgtgtgag gacttgagtt gaggatactt tatctcacag gcggagctgg ccggatgtat
    11821 tgcggaagct gcctgcgcga taacgccatg gccaccgagc tgatgaagcg tggtcacgac
    11881 gtgctgctcc tgccggttta cacaccgacg cttaccgacg aaccaaatgt cagcaccgat
    11941 cacgttgtgc tcggcggcat cagtgcatac ctcgaacaat atgtgccgct gtttcgcaaa
    12001 acgccgcgct ggctcgatcg tttatgggat tcaaagacgg ccttgaacct cgcgacgcgt
    12061 cgatcgctct cgaccaatcc gaagatgctg ggcgagatga ccgtttccgt gctgaaaggc
    12121 gaaggcggat ttcagcggaa agagatcgac aagttcatcg actgggttaa ggacgaagcg
    12181 ccgcctgacg tcgttagtct tccctacacc ctcctgctcg gcctggccca accaataaaa
    12241 cacgccttaa acgtacccat cctgtgcacg cttcaaggcg aagatctttt tctcgacaat
    12301 cttcaggagc cataccgcac gcaatcactg gatctgatcc gcgatcatct gcttcacgta
    12361 gatctgtttt tatccgtgtc agactattac gccgacttca tgcctggcta tctcggcatt
    12421 ccccgcgaga agatccgcgt cgtcccgatc gggatcaacc ctgaaggctt cgaattgcga
    12481 cacgctgatc gtagtggtcc attcacagtc ggcttcttcg gacgcatcgc tcctgagaaa
    12541 ggactacacg ttctcgccga agcctactgc atcgcacgtc aatccggaga actgcccgaa
    12601 gcgcgcttcg aagcggcagg ttatatggct ccggattcca agccgtatct cgaagccatc
    12661 caaaagcgcc tgcacgatgc cggtctaggt aatgagtttc actatcgcgg tgttttggag
    12721 cgcgcagaca agatcgcctt tcttcgtggc ctcgacctaa tgtccatgcc tgcgacctac
    12781 gacgagccga aaggcgtctc actactcgag gcaatggcct gcggtgtgcc gctggtccaa
    12841 ccacgccgcg gctcgttcac tgagatcatc gaaaggactg gcggcggtct cctggtccaa
    12901 cccgacgatc cccagagtct cgccgacggc cttctccaaa ttgctggcga ccaaaacctc
    12961 gcggcggatc tgagcgcaaa cggctttcgc ggcgttcgcg agcattacac tgccgtacac
    13021 atggccgata gagtcatgga atgctataac gaagcagtag caaatccgtc ttcatccgtg
    13081 ttaatccgtg gttaatatat gtttgtccca ggcgacgatc tgatattcca actcgaaagc
    13141 gctttcggac tgctgcgtgt gctggccgtc gaaggcgaag gcgcggagac agtgtggcac
    13201 ctgctggcct acgacgagtt tttccctgac gtcgaatcag cagaacagag gttggccgca
    13261 ggagttccgt tacctgttcg ccaagcgcac atggcgctca cgaatcgcgc cttcgagcgc
    13321 accccaaccg cgcgactcgg caaccgtccc gtcacagacg acgagctcgt cgcatacaac
    13381 acgtggctca acagcgatcg cacagtctcc gatcgctcag cactcttgat gctcggaatc
    13441 cgttaactat gccctcttct ttgcgtcctt tgcgcgtttg cgtctttgca ttattaattc
    13501 ttttcccgtt tacgaccttc gcttctcgta tcgagcgcct gatcgacacc tggcgtccga
    13561 cacactacct cgttaacatc acgctcaacg atcaactctc cgagatcgcg tccgcaaacg
    13621 cgcgcatcta tttgaccatc gtcaagccga ctcgcgagat cgatatcgac ttcggagatc
    13681 tgaccgtcga tcgagtcact ctcaatggca gctcgtccac cttccttcgc cgcgatggca
    13741 agctccactt tgatcttccg caagccgcca gcgcgggcga tgtctacatt atcgaggtct
    13801 actatcacgg caaaccgaaa gacggcttga ttctcaccaa cgacaaagac ggcaagcccg
    13861 ccgcgatcgg cgacaactgg cccgaccgcg tgcatcactg gatcccggta ctggaccatc
    13921 cgtcagcgaa agccaccgtc acattcaacg tcaccgcgcc tgcgggtcag gaagtggttg
    13981 cgaatggacg gttggaccac gtcgaaacga ctgcgagcgg ccagcgcacg tggacctact
    14041 cggaaggtgt gccgattccg ccgtattgca tgatcatcgg tgtgggccag tttgcacgcg
    14101 cggagtcggc gcagcgcgcg ctcacgccgc tttcgtacta cgtgccgtta tccgagcgcg
    14161 agctcgcgca aaaaggattc tctccggcga taccgacgat cgagttcttc actgagaagg
    14221 tcgcgccgta tccgtatgag aagcttgcgt tgattgtcgg cgcgacgcgt tatggcggca
    14281 tggaaaactc gggcgcgatc gtgtttacga gttctctgtt caaccgcaac tcgacggtcg
    14341 gcatgagtaa aacgtacggc attccttttg gtaacgtcac gctcatcgcg cacgagattg
    14401 cgcaccagtg gttcggcgat tcggtgacgg agtcgacgtg gtccgacttg tggttgagtg
    14461 aaggctttgc gacttacttc gcgggactgt ttgtgcagcg ttacgagagc gaagaggcgt
    14521 ttcagcgtta tatgcgcgac gcgtcggcga gtgtgtttgc gtacgagaag aagaagcgcg
    14581 tgccgatctt cgatcgcgac actgaaaatc ttatggagct tctgaatccg aacaattatc
    14641 agaagggcgc gtgggtgctg cacatgttgc gttcgaatct tggtgacgac gcattcttcc
    14701 gcggcatccg cgcgtattac gagtcgcata agaattcagt tgctagtacg gaggacttgc
    14761 gtgctgcgct tgagaagtcg tcgggcaaag atctgcgcac cttcttcgcg cgttgggttt
    14821 acgacacggg ccatccgcag tatgagttga agtggttctg gctcgggaag aaggaactcc
    14881 gcgtagttct aacgcaaaaa cagcccggca acgtcttcgt cgatcccatc cacctcacca
    14941 tctcaaccgc gaaaggcaaa cgcgacgtcg ttctcaagcc aacgggcaaa ctcttcatcg
    15001 agcgaatccc cctgcgcgac tctcccatca agctcgatct cgacccaaat aatcaactgc
    15061 tcgacgaatc aaccatcgct cccggctagg gtgaatttga caccttgagt ttgcttccct
    15121 atcatccatc ctcgtcgggg cgtggctcag cctggtagag cgcacggttc gggaccgtga
    15181 ggtcggaggt tcgaatcctc tcgccccgac caatcaattc aagcacttac gggcagcgca
    15241 tgctgcccgt tcccgtttga tgtggggact tttgtgtcgg tccagttttg aagctgcaca
    15301 accttactgt tccccactcc agtttgtgtt aataactgca tcgcacggcg aatgccgtca
    15361 cctgttgcgt gcgtgtaccg cattgccatg cgaatgtctg accaacccat gatttgagcg
    15421 atctcgaaag cgctggcccc acgatcacca agc
//
LOCUS       DASSFQ010000120        17148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1085978, whole genome shotgun sequence.
ACCESSION   DASSFQ010000120 DASSFQ010000000
VERSION     DASSFQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17148
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085978"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFH96_04020"
     rRNA            complement(<1..143)
                     /locus_tag="VFH96_04020"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(631..1179)
                     /locus_tag="VFH96_04025"
     CDS             complement(631..1179)
                     /locus_tag="VFH96_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HET6973164.1"
                     /translation="MCAGQLLGTRMALLGCRLLDIDDPRGADKKKLIVWVEIDRCMAD
                     AISAVTGVRLGKRSLKYVDYGKVAATFLNTETNRAVRIVALESSRTLADERYTDIADK
                     RTRQFRAYSEATDDELFKTELVSLELSEFDLPGSPRSRVTCAICEEGINDGREIIDAN
                     GDMICRGCQSGTYYSRLDNPTA"
     gene            complement(1206..1805)
                     /locus_tag="VFH96_04030"
     CDS             complement(1206..1805)
                     /locus_tag="VFH96_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973165.1"
                     /translation="MAQPIIIGVGGFTSNVGKTTLLCELLKAFPGWEAIKTTRGHYRS
                     CGKDPHACCVSHLLSAEPVVRSGRAETYSLGKDTGRYWDAGASNVHWLICTDNQVEEG
                     IKLALAKVKSPGVFVEGNSFTEFVNADHFVMCRQAGNSKIKSSAKRLISKASAIYVSD
                     TTDEPDDLQPFAIESIPLYTPASMFSLIDYLKWNRSKLS"
     gene            complement(1893..3020)
                     /locus_tag="VFH96_04035"
     CDS             complement(1893..3020)
                     /locus_tag="VFH96_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973166.1"
                     /translation="MRFSKTLFVFALLLLSLGCSTLANKSSTGVIVARRAQIRSSTAV
                     VAADLLQVNRGDVVDILEATDVTDPSDNARKERWLRVKARDSDTTEGWIEARNVMPDD
                     VLKESEKLAAEDKDIPAQATGQLRASSNLRLSADRSGNENIMMRLDSGSSFDIIGWKR
                     VPKPKSSEAIETDVAPKAGTAAQPGSGQSAAQREDDDKEPDETNELWYKVRLSGDASP
                     APAGWIYGKQVGLSVPSDIIFYRTGREFVGWQRLDGGDKEKTTKDAGKEDKPGSWLIL
                     EKSSSNQPHGIDEPDFDRIYVLGYDKRNQEHYTAYRSPDLKGYLPVRVEGQGESKSFT
                     IRLQEEEAGAVKEVRYSLYKDARGVLKINAPNIAPQGRRRR"
     gene            complement(3041..4456)
                     /locus_tag="VFH96_04040"
     CDS             complement(3041..4456)
                     /locus_tag="VFH96_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973167.1"
                     /translation="MNANQRGSAFIRGSFFIFLLALFSAGNVAAQNRVIISINDPSQI
                     KVDAELSRPSSSWSFPNAYAGVLGVAERIEEFRAVEADVRKAAVGEYRSERESTKISY
                     TVKLPKPTLADVSHVTWLAEDRGVLMLADLLPLEVRSLSATFVLPESWTVESSSPRDV
                     NGRYEVAEAEKAVFCIGKALRKSSTTVQGMPLDIVLSGNWPFKERDALERATKVMEKY
                     LALTGFRLRDKSVIVIAPLPITDSKTSWKAETRGSTVVLLINPDAGFKFWKSQLTIIF
                     THEVLHLWVPNSLKLEGDYDWFFEGFTMYTALRTALELGVIDFKEYLATLGRVYDQYR
                     AHPDTLSLIEESERRWASSDSPVYLKGMLVAFLYDLLMRKASGGRRTLADTYRDLFNG
                     RLTDGADGNDAIIGVLGSTPVSRSFIKAYVENSTAVDLNKVLPAYGMSVLFTGKGSQL
                     RVEGDLNQDQKQLLRSLGYQD"
     gene            4523..4897
                     /locus_tag="VFH96_04045"
     CDS             4523..4897
                     /locus_tag="VFH96_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973168.1"
                     /translation="MLTKAIQNRGATLKRGLGLLLVLFIFYGTTVEAAHRHGVAAPQS
                     NSATSVTNPNSGASSTFGTRPGCSDCLICQLHQNFSATLISVKLNTAPLARVTYALAF
                     DPVSIRSITHSSQSGRAPPQAN"
     gene            5035..7452
                     /locus_tag="VFH96_04050"
     CDS             5035..7452
                     /locus_tag="VFH96_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6973169.1"
                     /translation="MLFKIPFRAAIICGALILLGPTAFSQARIGSVQGVVKDPGGALV
                     PNATITVTQPITGYRQSTQTDAQGSFKLVNLPFNTYKVRAEANGFQPAEESIDLESTV
                     PFNVELALTLEQTTAAVTITTGGSILLEPDRTSSDTDINQTLLDRPLGAAPSRAIESI
                     VASTPGFVTDDNGRMHPRGSESQVQYVIDGVPVTDNMSAIFSTSLDARTLRTVEVLTG
                     GIPAEFGDKLAGVINVNTRSGLEGPTQGGISFSGGSFSTGEAAADFATHTKKLGFLMN
                     LSSSTSQRYLDPPTLDNFHNFGRTGKAFFRLDYQFDANNSLKGVFNFGGSNFQVPNRT
                     PQELAGQDQRQRLRDNSQNITFQHIFSPNSVAQFSFFHRQSNAKLISNPLSTPVVANQ
                     DRTLQNWGGIGSLALTRGKHNIKVGGQFTITPVDEHFTFYPTTAFPDLEDEEGNVFPN
                     PVNNFNVATPFVFDQSKTGRTLSAYVQDRFTVFKNLTLDLGVRYDNYKLLISEQAVSP
                     RLGFAYYLPKTKTTLRASYNRLFQPPPAENLLLASSNEAAALSPIAVLQGVTTVRPIL
                     PDKQHAYEAGAQQLLSKFFRLNLTVYQKRIENFSDKDQFFETGVIFPIAISSGRVTGE
                     EVRLESTEIHGFRTFVSYANARAFGVTPITGGLFLGEDPQDLFLNGFKFANDHDQRNE
                     AQFQVSYNHRPSGLYASFNGRYDSGVPTDVEPGTTLADFVAQGFDPRLFNEIDFQRGR
                     VKPRMVLDFSVGADLMQKERVSMNVQFDVQNLTNELFLYNFESVFSGTHVGYPRLFSG
                     KLSLRFK"
     gene            7565..8686
                     /locus_tag="VFH96_04055"
     CDS             7565..8686
                     /locus_tag="VFH96_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973170.1"
                     /translation="MLRPEPRGFSREPGQDHRHHDNRYSQHSALRPFFRLAERLLLRF
                     ATILLLACFYVAVQPTTALAQQNADDEDVIRVNTDLLLFPVRIRDKKGLAVAGLSDQD
                     LSLKDPDQVTTGLYFSRGADRVALMFGLDQSGSLRQIISQQQEAALALFSRFGERSSV
                     AILRFSDRATLVAPFTKDLDAARAAFRFRAGSNEQTAIFDAAAKAVTAYDDLPPVRSE
                     RRIIVLISDGLDTRSRTSADSVINAALERRVSFYVIHLPLFEPRDGRLQVRSPSKGFK
                     DLAEKTGGKYFLVGDAKSALMPRENDLTPIFQAIEEDLKSQYLLGFYISESSRDNRKH
                     VFSVSMKPEGVEYSVGGKGYSRTQKFMINLAPGAKKLPN"
     gene            8695..10239
                     /gene="purH"
                     /locus_tag="VFH96_04060"
     CDS             8695..10239
                     /gene="purH"
                     /locus_tag="VFH96_04060"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET6973171.1"
                     /translation="MTENLRPIQRALISVSDKTGILDFARELTRFNVEIISTGGTAKA
                     LRDAGITVRDISDVTGFPEMMDGRVKTLHPRVHGGLLAIRDNADHAAALEQHGIEPID
                     LVVVNLYPFAETIKREGVTRAEAIEQIDIGGPAMIRSAAKNARDVAVVVSPEQYAQVI
                     EELNQNHGALSLNTRNTLAQKAFEHTAQYDLMVSSYLAGSKDVLPDRLNWTMTKIADL
                     RYGENPHQKAALYQTSTSGGIANAAVLAGKEMSFNNYVDAEAAWQLVCDFDQTACAII
                     KHTNPAGAGLGKTLADAYKKALATDPVSAFGGIVAFNRTVDAAAAEEVTKIFTEVVIA
                     PGYDAAALEVLRAKKNLRVIQMSDVEQPVGVELKQIGGGVLVQTRDDHRLDPAELNVV
                     TTRKPTDDEIRALQFAWIVCKHTKSNAIVYARADQTVGVGAGQMSRVDSVKIGAMRAQ
                     LPVKGSVLASDAFFPFRDGIDEAARHGITAVIQPGGSIRDEEVIAAANEYGLAMVFTG
                     IRHFKH"
     gene            10321..11298
                     /locus_tag="VFH96_04065"
     CDS             10321..11298
                     /locus_tag="VFH96_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6973172.1"
                     /translation="MADNQDLINNIPSIRCRYCGQRNQIRTESGEARCGRCRLPLSDE
                     PPHKKFANLDKDQYIHPADRRALAALRAIPGIDTALKKLLAVTGESAIRVIFTASAVK
                     VTPTQCPDLYAKLQIACTTLGVDMPDLFVQQNPIVNAFTGGVERPVIVLYSQLIERLS
                     DDEVLAVVAHEVGHIHAEHVLYLTAARLIEFLAKSAVLASPLTFIIKELLTMSMRAAL
                     LAWARRAELSCDRAALLVTQDANVIGRTMMKLSGGTFASRVDYDQFLGQARDFQKNYD
                     EKALDRFWADVINSGLSHPFPVWRVSEILQWVESGEYKALMNAPESAAA"
     gene            11344..12108
                     /locus_tag="VFH96_04070"
     CDS             11344..12108
                     /locus_tag="VFH96_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET6973173.1"
                     /translation="MLNNKNGIIFGVANKRSIAWATALALHEAGAKLAFTYQGERLKE
                     NVESLTNDAMPGSLLLSCDVTNQEEVDKTFQQVGEQFGRLDFLIHSIAFAPREALGGE
                     YLNTSREAFLTALEISAYSLPQLARAASPLMTEGGSIVSMSYYGAEKVVAGYNVMGVA
                     KAALEASTRYLAKDLGPRNIRINAISAGPIQTLSARGVSDFGTMLKHHAERAPLGRNV
                     EPREVGNTAVFLCSSMSSGITGEVIYVDCGYNIMGI"
     gene            12108..12869
                     /locus_tag="VFH96_04075"
     CDS             12108..12869
                     /locus_tag="VFH96_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET6973174.1"
                     /translation="MEKNRKGTGRALPGTTQDEQLLESPRADEFTHTDPWRVFRIMGE
                     FVEGFDELATLSRGIAIFGSARTRPDDPEYKAAQETGALLAAQGFAVITGGGPGIMEA
                     ANRGAFDAGGLSIGCNIELPFEQKPNAYQTLSLTFKYFFVRKMMFVKYSLAFVIFPGG
                     FGTLDELFEALTLIQTKKIRNFPVVLFGSQYWSGLLDWLRAVVLPDGKISEHDMDIFH
                     ITDSPAEVVEIVTRSQSSLHQLDNTISDEIRISSV"
     gene            complement(12858..13187)
                     /gene="cutA"
                     /locus_tag="VFH96_04080"
     CDS             complement(12858..13187)
                     /gene="cutA"
                     /locus_tag="VFH96_04080"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET6973175.1"
                     /translation="MSDDAIVVFMTAANGEEATRLAEMLVGSHLAACVQILPEIESVY
                     RWQGKIERSSEVLLLAKTTRGKFDDLEREVRALHSYDTPEIVAVPIVAGSAPYLEWLS
                     QATDLHG"
     gene            complement(13194..13580)
                     /locus_tag="VFH96_04085"
     CDS             complement(13194..13580)
                     /locus_tag="VFH96_04085"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET6973176.1"
                     /translation="MICPVCEGEYEPGITQCPTDDTPLVDKITSTHDDSEASLVTLHN
                     FGSPAEAEMVNDILTQNGIRSMVKSGGADALSPLLSTTWEGAQILVDERDLDRAREVY
                     QSFFGADTTPLTGGTIDDDLEDEDEE"
     gene            complement(13592..14518)
                     /locus_tag="VFH96_04090"
     CDS             complement(13592..14518)
                     /locus_tag="VFH96_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6973177.1"
                     /translation="MSLLVVGSVAYDEVETPFGKCDKMLGGSASHFSISASFFTKVSI
                     VAVVGGDFAEQEVFDSRGIGTSDIERIADGKTFRWGGRYEYDLNVAHTLYTDLNVFAD
                     FQPKLSEESKRTRLLFLGNIQPDLQRGVREQMPDAELVALDTMNLWIEHTRDSLVKTI
                     KVVDVVIINDAEARQLTEIPNLIKAAREILSWGPKALIIKRGEYGAALFTRDSYFAIP
                     AYPLESVFDPTGAGDTFAGGFMGYLASQETIDESAMRRAMIFGSVMASFNVEEFGTER
                     VRRLTHAEINDRFHAFKRMTHFDEIPFEAKLS"
     gene            complement(14546..15031)
                     /gene="bamD"
                     /locus_tag="VFH96_04095"
     CDS             complement(14546..15031)
                     /gene="bamD"
                     /locus_tag="VFH96_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET6973178.1"
                     /translation="MKSKFLFVFLILFVLFVAPSTFAQGGVKGGPDPSTLRDPDLERD
                     SLHNLEVAKNYFKLKKAYVAALKRCEEIIAGNPNFQRIDEVLLMAGQSSLWLSQNKGK
                     QRPEQYVSFDGSEKKTLTTAQFREMGVDYLNKLIKDYPESPFAQEAREELRLLEAPKQ
                     Q"
     gene            complement(15047..15928)
                     /locus_tag="VFH96_04100"
     CDS             complement(15047..15928)
                     /locus_tag="VFH96_04100"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET6973179.1"
                     /translation="MSTAAGVRVGIIGGSGLYQMPELTAIEEVKVDTPFGPPSDAFIV
                     GTLEGERVAFLPRHGRGHRLTPTEVPFRANIYAMKLLGVESILSASAVGSLQEQYAPL
                     DMVIPDQFFDRTRGRVKESTFFGEGIVAHVSFAHPVCGRLGDVLQEACGAADVKAHRG
                     GTYLCMEGPAFSTVAESNVYRSWGMDIIGMTNLQEAKLAREAEICYATLALVTDYDCW
                     HPGHDAVSVETVVEYLNKNVRNAQIILREAVKGMRSSDRSCKCGSALQHAIFTAPNLW
                     PEATTKKLEAIIKKYAS"
     gene            complement(16007..16891)
                     /locus_tag="VFH96_04105"
     CDS             complement(16007..16891)
                     /locus_tag="VFH96_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6973180.1"
                     /translation="MNFGDYRVEIIPDTEFRLDGGAMFGVVPRKLWAREHPPDDDNRI
                     RMNMNCVFIDTGAEKILIETGIGDKWTAKQESMYGIDRVRPLSETLFAHTGATAAEIT
                     IVINTHLHFDHAGGNTKLDAEDNLVASFPNARYFVSGAELEHADRPTERDRASYLPEN
                     WRPLVESGQLEAKDSDYEVVPGLTMETIAGHNRSMQCWRLERDGKTMFGFADLVPMRA
                     HVRLPWIMGYDLYPVETLDAKKRLIPQAARENWICMFYHDPDEPLCRLQQHEKEFSPI
                     PYRGDSSSSLSLQVSDKL"
     gene            complement(16937..>17148)
                     /locus_tag="VFH96_04110"
     CDS             complement(16937..>17148)
                     /locus_tag="VFH96_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973181.1"
                     /translation="AAVEQEKAKKVLDEIVSRSHVKVPDTYTVKPPEQQPQQALPPGF
                     GAPPQEAPAPAPSPAKPSATKPKGH"
BASE COUNT         3909 a         5024 c         4371 g         3844 t
ORIGIN      
        1 cccaatccaa aggtagatta cctacgtgtt actcacccgt gcgccactct acttgctccc
       61 cgaagggaac tttcgcgttc gacttgcatg tctgaggcac gccgccagcg ttgattctga
      121 gccaggatca aactctcgtt ataagtttgt tctctggaac attatctatt actggtcttt
      181 cttgttcagt ttcgataatg actcttgcga gttttacgcg aactttatcg ttcacgtttt
      241 gactctcaaa ggtcttgaaa cggacacgct ctatctagtt ttcaaacatc gattccaaat
      301 tgtatttgga ttgccctaca gacgattctg actcgccgca agcaaacggg gatattaacc
      361 aacgacctcg actctgtcaa gcgactaagc ccttattctc gatttattca gagaataaaa
      421 agcgccgcaa tttcctcgca atctttcgag gaaaagcgac acataaggtc gtctcgaatt
      481 ccctgttctc aaaccccttg ataccctctc gttagaggaa tgggcattat agggataaaa
      541 tcctacttgt caacaccttc tgacgctcgc agtaaaaaat ttttgcttgc gtgaagcccc
      601 acgttttcag aggcgggtct tagatgtcac ttatgcagtc gggttgtcca gcctggaata
      661 ataagttccg ctctgacagc ccctgcaaat catgtctcca ttagcgtcaa taatctctct
      721 gccgtcgtta ataccttctt cgcaaatagc acaagtcaca cgcgagcgcg gactgcccgg
      781 aagatcaaac tcgctcaatt caagactcac caactccgtc ttgaacaact cgtcatcggt
      841 cgcttcagag tacgcgcgaa actgccgcgt acgcttgtca gcgatatccg tataacgttc
      901 gtcagcgaga gtgcgggagc tttcaagcgc gacgatccgc acggcgcgat tcgtctcagt
      961 gttcaaaaat gtcgccgcta ctttgccgta atcaacatac ttcaaacttc gcttaccgag
     1021 acgcacgcca gtgaccgcgc taatcgcgtc agccatgcag cgatcgatct ccacccaaac
     1081 gatcagcttc tttttatccg cgccgcgcgg atcatcaatg tccagcagac ggcaaccgag
     1141 caacgccatg cgtgtgccga gcagttgtcc tgcgcacatg tgcccgtgca gccgctcgca
     1201 ttcttttaag agagtttcga ccgattccat tttagataat caatgagaga gaacatgctc
     1261 gcgggggtgt agaggggaat cgattcgatt gcgaagggtt gcaagtcgtc gggttcatcg
     1321 gtcgtgtcag aaacgtaaat agcgctcgct ttactaatga gacgctttgc tgagctcttg
     1381 atcttggaat taccagcctg ccggcacatc acgaagtgat ctgcgttcac gaactcagta
     1441 aagctgttgc cttcgacgaa cacgcccggt gatttcactt tggcgagcgc gagcttaatg
     1501 ccttcttcga cctgattgtc agtgcagatt agccagtgaa cgttggaggc gcccgcgtcc
     1561 cagtaacgtc ctgtgtcttt gccgagggaa taggtttctg cacggcctga gcgcacgacg
     1621 ggttctgcag ataagagatg acttacacaa catgcgtgag gatctttgcc gcaggagcga
     1681 taatggccgc gcgttgtttt gattgcttcc cagccgggaa aggctttgag taattcacag
     1741 aggagcgtcg tcttcccgac gttggaagtg aatccgccga caccgatgat gattggttgg
     1801 gccatgattc ccaagtcagt cttttacagg attaacaaga tcgaccaaga tttacatgta
     1861 aatcctgaaa atcttgcaaa tcctgtctaa aactacctcc gtctcctacc ctgcggggca
     1921 atattcggcg cgtttatctt caacacgccg cgcgcatcct tgtaaaggct gtaacgcact
     1981 tccttcactg ctccagcttc ctcttcctgc aagcggatcg tgaagctctt actctcgccc
     2041 tgcccctcga ctctcaccgg taaataaccc tttagatccg ggctgcgata cgctgtgtag
     2101 tgctcctgat tgcgcttgtc ataaccgagt acatagatgc gatcgaagtc gggttcgtca
     2161 attccgtgcg gttgattcga gctgcttttc tcgagaatca accagctacc gggcttgtct
     2221 tctttccccg catccttcgt cgtcttttcc ttgtcgccac catcgagacg ctgccaaccg
     2281 acaaactcgc gccccgtgcg gtaaaaaatg atgtcactcg gcaccgaaag cccgacttgc
     2341 ttgccgtaaa tccaaccagc cggggcgggc gacgcgtcgc cggaaagcct cactttgtac
     2401 cagagctcat tcgtctcgtc cggttccttg tcgtcatctt cacgctgtgc tgccgattgt
     2461 ccgctgcctg gttgtgccgc ggtgccggcc ttcggcgcga cgtcggtctc gatcgcttcg
     2521 gaagatttcg gtttcggcac acgcttccag ccgatgatgt cgaaactcga tccactatcg
     2581 agccgcatca tgatgttttc attgccactg cgatccgcgg agagtcgcag gttggaactc
     2641 gcacgaagct ggcccgtcgc ctgagccgga atgtccttgt cttccgcggc taatttctcc
     2701 gactccttga gcacgtcgtc aggcatgacg ttgcgcgctt cgatccaacc ttcggtagta
     2761 tcactgtcgc gagctttgac gcggagccat cgttcttttc gtgcgttgtc gctgggatca
     2821 gtgacgtcag tggcttcgag tatgtctaca acgtctcccc gattcacttg caacaaatcc
     2881 gccgcaacaa cggcagtcga gctgcgaatc tgtgcgcgcc gcgcgacgat aacacccgtg
     2941 ctgctcttat tcgcgagggt cgaacaacca agtgacaaca acagcagcgc gaaaacaaaa
     3001 agggtcttgc taaatctcat tcgtgttcct tcgagtctga tcaatcctgg tagccaagcg
     3061 atcgaagcaa ctgcttctga tcctgattca ggtcgccctc gactcgtagc tgcgaacctt
     3121 taccggtgaa tagaacggac attccatacg cggggagaac tttattcaga tccaccgccg
     3181 tgctgttttc aacataagct tttataaaag agcgggagac gggcgttgaa cccagcacgc
     3241 cgattatagc gtcatttccg tctgcgccgt cagtcaaccg gccgttaaag agatctcgat
     3301 aggtgtctgc gagagttcgc cggccaccgg atgctttccg catcaacaag tcatacagaa
     3361 acgcgaccag cattcctttg agatacacgg gggaatcgga gctcgcccaa cgacgctcgg
     3421 attcctcgat gagcgatagc gtgtccggat gggcccggta ctgatcgtaa acacggccga
     3481 gagtagccag atattctttg aagtcgatta caccgagctc aagcgccgtg cgcagggcgg
     3541 tatacatggt gaacccttcg aagaaccaat cgtagtcgcc ttccaacttc aaagagttag
     3601 gcacccaaag gtgcagaact tcatgtgtga agatgatcgt gagctgcgat ttccagaact
     3661 taaagccggc gtctggattt atcagcaaaa cgactgtgga gcctcttgtt tcagctttcc
     3721 aacttgtctt ggaatctgta atcggaagag gcgcaatcac gatcactgac ttgtcgcgca
     3781 gacggaatcc agtcagcgcc aggtatttct ccatcacctt tgtcgcgcgc tccaacgcat
     3841 cgcgttcttt gaatggccaa ttcccgctga gaacgatatc cagcggcatt ccctgtacag
     3901 tcgtcgaact ctttcgcagc gctttcccga tacagaaaac tgctttctca gcttccgcga
     3961 cctcgtaacg accattcacg tcgcgtggag aagacgattc aacggtccaa ctttccggta
     4021 gaacgaacgt ggctgaaagg ctacggactt caagcggaag caaatcagcg agcatcagaa
     4081 caccgcgatc ctcagcgagc caggtgacgt gtgagacatc agcgagcgtg ggttttggaa
     4141 gcttaactgt gtaactaatc ttggtcgact cgcgctcgga tcgatactcg ccaaccgcag
     4201 ctttccgaac atccgcctcc acagcgcgaa actcttcgat cctttccgca actccgagaa
     4261 ctcctgcata cgcattggga aaggaccacg agctgctcgg tctcgatagc tccgcatcga
     4321 ctttaatctg acttggatca ttaatcgaga tgattacacg attctgagcc gcaacattac
     4381 cggcgctgaa caatgccagc aaaaagatga aaaaactgcc gcggataaac gcagatccgc
     4441 gctgatttgc gttcatccgc ggccgctttt ctatttctgg agaagatttc acgggcgatg
     4501 ttgtgtacaa tcccgaatca gcatgttaac gaaagcaatt cagaaccgcg gcgccacact
     4561 aaaacgcggc ctcggtctgt tgctggtcct cttcatcttt tacggtacca ctgtcgaggc
     4621 tgctcaccgc cacggagtcg cagcgcctca gtcaaacagt gcgacttcag taacaaatcc
     4681 gaactcgggc gcgagcagca ctttcggcac caggcccgga tgcagcgact gtcttatctg
     4741 tcaactgcat caaaatttct cggcgacgct gatctccgta aaactcaaca ccgcaccgtt
     4801 agctcgtgtc acctacgcgc tagcgttcga ccccgtttca attcgctcga taacccatag
     4861 ctcacagtca ggtcgagcgc cacctcaagc caactagaac cgcccacttc gtgtggtggt
     4921 ggtttcacta cgcgaagtac cactgcggac tgagcaaccc agttctccag caggagaaaa
     4981 ctgcgtggcc atgcccgcat ccgcttacac aggcatttct ggaggcttga tgcaatgtta
     5041 ttcaaaatcc cgttcagggc cgcaatcatt tgcggtgcac ttatcttact tggtccaact
     5101 gccttctcgc aggcccgaat cggcagcgtt cagggcgtag ttaaagatcc gggcggcgcc
     5161 ctcgttccca atgcgacgat caccgtcact caacccataa ccggctaccg gcaatcaact
     5221 caaaccgacg cgcaaggctc gttcaagctc gtcaatttac ctttcaatac gtacaaagtc
     5281 cgcgccgaag ccaacggctt tcaacccgca gaggaatcga tcgatctcga aagcacggtt
     5341 ccctttaacg ttgagctcgc actgacgctc gagcaaacga ccgccgcagt cacgatcaca
     5401 accggcggct cgatactgct cgagcccgat cgcacgtcat cagatacaga cataaatcaa
     5461 acgcttctcg atcgcccact cggcgcggct ccgtctcgcg cgatcgaatc catcgtcgcg
     5521 tcgacaccgg gctttgttac cgacgacaac ggccgcatgc atccacgcgg ctccgaatcg
     5581 caggtgcaat acgtgatcga cggcgttcca gttacagaca acatgtcggc aatcttttcg
     5641 acgagtcttg atgcacgaac attgcgcaca gtggaagtgc tcaccggcgg tatcccggca
     5701 gaatttggcg acaagctcgc aggcgtgatc aacgtcaaca ctcgctccgg actggaagga
     5761 ccgacacagg gcggaatctc tttttcaggc ggcagtttct caacgggcga ggccgcagcc
     5821 gacttcgcta cgcacacgaa gaaactcggt tttctgatga acctgtcctc cagcacgtcg
     5881 cagcgatatc tcgatccacc aacgctcgat aactttcaca acttcggacg cacaggcaaa
     5941 gcattcttcc gtctcgatta ccagtttgac gcaaacaaca gtctaaaggg cgtcttcaat
     6001 ttcggcggct cgaatttcca ggtgccgaac agaactcctc aagaacttgc aggacaggac
     6061 cagcgacaac gtctccgcga caactcgcaa aacatcactt tccaacacat tttctcgcct
     6121 aactctgtcg cacagttctc gttcttccac cgccaaagca acgccaaact gattagcaat
     6181 ccgctctcga cgcccgtagt cgcgaaccag gatcgcacgc ttcagaactg gggcggcatc
     6241 ggctcgctcg cgcttacgcg tggcaagcac aacatcaaag ttggcgggca attcacgatc
     6301 acgcctgtcg acgaacactt caccttttat ccgacaacag cgtttcccga tctcgaggat
     6361 gaagagggaa atgtttttcc gaacccggta aacaatttca atgtggcgac tccgttcgtc
     6421 ttcgatcagt cgaaaactgg ccggacgctg agcgcttacg tgcaggatcg tttcactgtt
     6481 ttcaagaacc tgacgttgga cctcggtgtg cgttatgaca actacaaatt gctgatcagc
     6541 gagcaggctg tcagcccgcg cctgggtttc gcttattacc tcccgaagac gaagacgacg
     6601 ctgagagctt cttacaatcg actgtttcag cctccaccag ccgagaacct tttgctggcg
     6661 agctccaatg aagcagccgc gctctcgccg atcgcagtcc tgcagggcgt gaccacggtg
     6721 cgaccgattc tgccggacaa acagcacgct tacgaagccg gcgcacaaca gctactctcg
     6781 aagttctttc gactgaacct gacggtctac cagaagcgaa tcgagaactt ttctgataag
     6841 gaccaattct tcgagactgg cgttatcttt ccgattgcca tctcgtcagg acgagtcacg
     6901 ggcgaagaag tccggctcga gtcgaccgag attcacggct tccgtacctt cgtctcgtat
     6961 gccaatgcac gggcgttcgg tgtgacgccg atcacaggcg gactgttctt aggtgaagac
     7021 ccgcaggact tgtttctgaa cggcttcaag ttcgcgaacg accatgatca acgcaacgag
     7081 gcgcaatttc aggttagtta taaccatcgc ccgtcaggac tgtacgcgag tttcaacggg
     7141 cgctatgact ccggcgtccc gacggacgtc gagcccggaa cgacactcgc ggatttcgtc
     7201 gcccagggct tcgatccgcg tttgttcaac gagatcgact ttcaacgcgg gcgagtgaag
     7261 ccgcgaatgg tgctggactt ctccgtcggc gcggacctga tgcagaagga acgcgtgtcg
     7321 atgaacgtcc agtttgacgt gcaaaacctg acgaatgagc tgtttcttta taattttgaa
     7381 tcggttttca gcgggacgca cgtcgggtat ccgaggctct tcagcgggaa acttagctta
     7441 aggtttaaat aggttttggc cgcggattta cgcagataaa cgcagatcag cgttaatccg
     7501 cgttggatcc gcggctaatt tctctgaacc ggctcgaaga tagggacgta tgctagaatg
     7561 cgctttgctt cgcccagaac cccgaggatt cagccgtgag cctggtcaag atcatcgtca
     7621 tcacgataat cgttattctc agcattctgc tcttcggccg ttttttcgtt tagccgaacg
     7681 gctcctgctg cgtttcgcga ctattcttct gctcgcctgt ttttacgttg cagtccaacc
     7741 aaccactgca ctcgcacagc aaaacgctga tgacgaggac gtcattcgtg tcaacactga
     7801 cctgctgctc tttcccgttc gcattcgcga caaaaaggga ctcgcggtcg cgggcctgag
     7861 cgatcaggac ctttcgctga aagatccgga ccaggtcacg accggtttgt acttctctcg
     7921 cggcgcggat cgcgtggcct tgatgttcgg actcgaccag tcaggcagcc tgcgccaaat
     7981 aatctcgcaa cagcaggaag ccgcgctcgc gctgttctca cgctttggcg aacgatcgag
     8041 cgtcgcgatt cttcgttttt cagacagggc tacactcgtc gctccattca caaaagatct
     8101 cgacgccgcg cgcgctgcat ttagattcag ggccggatct aacgaacaaa ccgcgatctt
     8161 cgatgccgca gcaaaggccg ttacagccta tgacgatctg ccgcccgtgc gctctgagcg
     8221 tcgcatcatc gtgctgatca gcgacggcct cgacacgcgc agccgcacaa gcgctgacag
     8281 tgttatcaat gctgccctcg agagacgcgt ctcgttttat gtaattcacc tgccactctt
     8341 cgaaccgcgc gacggtcgac tccaggtgcg cagcccgagc aagggtttca aagacctcgc
     8401 cgagaagacc ggcggcaaat attttctcgt gggcgacgcg aagtcagccc tcatgcccag
     8461 agagaacgat ctgacgccga tctttcaggc catcgaagag gatttgaaaa gccagtacct
     8521 tttgggtttc tatatatccg aatcgtcgcg cgacaatcgc aagcatgttt tctctgtgag
     8581 tatgaagccg gaaggtgtcg agtactccgt cggcggcaaa ggttattcgc gcacacaaaa
     8641 gttcatgatt aatctggcgc ctggagcaaa gaaactccct aattaacttc gttcatgact
     8701 gaaaatcttc gacctattca acgcgcactg atcagcgtct ctgacaaaac cggcattcta
     8761 gacttcgctc gcgagctcac gcgcttcaac gtagaaatca tcagcacggg cggcactgct
     8821 aaagcactgc gcgatgcggg tatcaccgtg cgcgacatct cagacgttac cggctttccg
     8881 gaaatgatgg acggccgcgt caagacacta catccacgcg tgcacggcgg actgcttgcg
     8941 attcgcgaca acgcagacca cgccgccgcg ctcgagcagc acggaatcga accgatcgat
     9001 ctcgtcgtcg tgaatctcta tcccttcgca gagacgatca aacgcgaggg cgtgacgcgt
     9061 gcagaagcga tcgagcagat tgacatcggt ggtccagcaa tgattcgctc cgccgcgaag
     9121 aacgcgcgtg acgtcgccgt ggttgtctct cctgaacaat atgcgcaagt gatcgaagag
     9181 ctaaaccaaa accacggcgc gctgtcgctc aacacgagaa acacgctcgc tcaaaaagca
     9241 ttcgagcaca ccgcgcaata cgacctcatg gtcagctcat atctcgccgg ttccaaggat
     9301 gtgctgcccg atcgcctgaa ctggaccatg acgaagatcg ccgatctgcg ttacggtgaa
     9361 aacccacacc agaaagccgc gctctaccag acttcaacga gtggcggcat cgcgaacgct
     9421 gcggttctcg cgggcaaaga gatgtcgttc aacaactacg tcgatgccga agccgcgtgg
     9481 caactcgtct gcgatttcga tcagaccgcg tgtgcgatca tcaagcacac aaatccggca
     9541 ggcgcaggtc taggaaagac tctggccgat gcctacaaga aagctttggc gacggatcct
     9601 gtatcggcgt ttggcgggat cgtcgcattc aatcgcacgg ttgatgcggc agcggcggag
     9661 gaagtgacga agatcttcac cgaggtcgtg atcgcgcccg gctacgacgc agccgccctc
     9721 gaagtcctgc gcgcgaagaa aaacctgcgt gtgattcaaa tgagcgacgt ggaacaacca
     9781 gttggagtgg agttgaagca gatcggtggc ggcgtgctcg tgcaaaccag agacgatcat
     9841 cgacttgatc ccgcggagct caacgttgtc acgacgcgga agccgactga cgacgagatc
     9901 cgcgcgctac agttcgcatg gatcgtttgc aagcatacga aatcaaacgc gattgtttac
     9961 gcacgtgcag atcagaccgt cggtgtcggt gcgggacaga tgtcgcgcgt tgactccgtg
    10021 aagatcggtg cgatgcgagc gcagttgccc gttaaaggat cagttttggc atcggacgca
    10081 ttcttcccat ttcgcgacgg catcgacgag gcggcgcgac acggaatcac cgcggtgatc
    10141 caaccgggcg gctcgatccg tgacgaagaa gtgatcgccg ccgccaacga gtacggcctc
    10201 gcgatggttt tcaccggcat ccgtcatttc aaacattaat tagccgcgga tttgcgcgga
    10261 tcagtgacgg atccggcttt tcatccgtat aatccgtggc accggaagtg agcgtttaat
    10321 atggctgaca atcaagactt gattaacaac atcccctcga tccgctgccg ctactgcggt
    10381 cagcgcaacc aaattcgcac ggaatccggc gaagcacgct gcggccgttg ccgtctgcct
    10441 ctctccgacg agccgccaca caagaaattc gcaaatctcg acaaggacca gtacatccat
    10501 ccagccgatc gccgcgcgct cgcagcgctg cgtgcgatcc ctggaatcga cacggcgctc
    10561 aagaaactgc tggccgtcac cggtgagtca gccattcgcg tcatcttcac tgccagcgca
    10621 gtgaaagtca cacccactca gtgcccggat ctctacgcca aactccagat cgcctgcacg
    10681 actctcggtg tcgacatgcc cgatctgttc gtgcaacaaa acccgatcgt caacgcgttt
    10741 acgggtggcg tcgagcgccc tgtcatcgtg ctctattccc aactgatcga gcggctgagc
    10801 gacgacgaag tgcttgcggt cgtagctcac gaagtgggcc acattcacgc cgagcacgtg
    10861 ctttacctga ccgccgcgcg tctgatcgaa tttttggcga agagtgcagt gctcgcatcg
    10921 ccgttgactt tcatcatcaa ggagctgctc acgatgagca tgcgcgctgc attgctcgcc
    10981 tgggccaggc gcgcggaact gtcgtgcgat cgtgccgccc tgctcgttac acaagacgcc
    11041 aacgtcatcg gccgcacgat gatgaaactc agcggcggca cttttgcgtc gcgtgtcgat
    11101 tacgatcagt tcctcggaca agcgcgcgac tttcaaaaga actacgatga gaaagcgctc
    11161 gatcgtttct gggccgacgt gatcaattcg ggcctgagcc acccgtttcc ggtgtggcgc
    11221 gtctcagaga tcctgcaatg ggttgagagc ggagaataca aagctctgat gaacgcgccc
    11281 gaaagcgcag ccgcataaat aaatataggt cctataggac caataggacc tataccgaac
    11341 aacatgctaa ataacaaaaa tggcattatc tttggagtcg caaacaagcg ctccatcgcc
    11401 tgggccactg ccctcgcact tcacgaagca ggcgctaagc tcgctttcac ttatcagggc
    11461 gagcgcctca aagaaaacgt cgaaagcctg acgaatgacg cgatgcccgg gtcgttgctg
    11521 ctctcgtgcg acgtgacgaa tcaggaagaa gtcgataaaa catttcaaca agtcggcgag
    11581 cagtttggcc ggctcgactt cctgattcac agcatcgcct tcgccccgcg ggaagcgctc
    11641 gggggcgaat acctgaatac ctctcgcgaa gcttttctga ccgcactcga aatcagcgct
    11701 tactctctgc cgcaactcgc acgcgccgcg tcgccgttga tgactgaagg cggcagtatc
    11761 gtctcgatgt cgtattacgg cgctgaaaaa gtcgtcgcgg gctacaacgt gatgggcgtc
    11821 gccaaggccg cactcgaagc cagcacgcgc tatctcgcaa aggatcttgg accacgaaac
    11881 attcgcatca acgcgatcag tgctggtcca attcaaacac tctctgcgcg cggcgtctcc
    11941 gatttcggca ccatgctgaa acatcacgcc gaacgcgcac cgctcggccg caacgtggaa
    12001 ccgcgcgaag tcggaaacac cgccgtcttc ctttgcagct cgatgtcttc cggcatcacc
    12061 ggcgaggtga tctacgtcga ttgcggctac aacatcatgg gcatctgatg gaaaagaatc
    12121 gaaaagggac cggccgcgca cttcccggaa caacacaaga cgaacaacta ctcgagagtc
    12181 cgcgtgcgga cgagtttaca cacacagatc cgtggcgcgt tttccgtatc atgggcgagt
    12241 ttgttgaagg cttcgacgaa ctggcgacac tgagccgcgg cattgccatc ttcggctccg
    12301 cgcgcacccg gcctgacgat cccgaataca aagccgcaca ggaaaccggc gctcttctcg
    12361 ccgcgcaggg ttttgcagtg atcacaggcg gtggtccggg aatcatggaa gccgcgaatc
    12421 gtggcgcgtt cgatgcgggt ggactctcga tcggctgcaa catcgagctg ccgttcgagc
    12481 agaaaccaaa tgcttaccag acactcagtc tcacgttcaa atacttcttc gtgcgcaaga
    12541 tgatgtttgt taagtacagt ctcgccttcg tgatctttcc gggagggttt ggcacccttg
    12601 acgagctctt tgaagcgctg acactaatcc agacgaagaa gattcgtaac tttccggtcg
    12661 tactgtttgg aagtcaatac tggagtggac tgcttgattg gctgcgtgcg gtagtgttac
    12721 cagatggcaa gatctccgag cacgacatgg atatctttca catcactgac tcgcctgccg
    12781 aggttgttga gatcgtaact cgaagtcaaa gctcactcca ccagctcgat aatacgatct
    12841 cagacgaaat ccgtatctca tccgtgtaaa tctgtggctt gagataacca ctcgagataa
    12901 ggcgccgatc cggctacgat aggaacagca acaatctcgg gtgtgtcgta ggaatgcagc
    12961 gctcgcactt ccctctcgag atcgtcaaac ttcccgcgcg tcgtcttcgc gagcaaaagc
    13021 acttctgaac ttcgctcaat ctttccctgc caacgataaa ccgactcgat ctccggcagg
    13081 atctgaacac acgcagcgag gtgagaacca accagcatct ccgcgagccg cgtcgcttcc
    13141 tcaccattcg cggccgtcat aaaaactaca atcgcatcgt cagacatcgc gaactactct
    13201 tcatcttcat cttccaggtc gtcgtcgatt gtaccaccag taagcggtgt cgtgtcggcg
    13261 ccgaagaagg actggtagac ttcccgcgcc cgatccagat cacgctcatc gacgagaatc
    13321 tgggcgcctt cccaggtcgt agaaaggagc ggcgacaatg cgtcagcacc tccggatttc
    13381 accatcgagc gaatcccgtt ttgagtgaga atgtcgttca ccatctcggc ttcggcaggt
    13441 gaaccgaagt tatggagcgt aacaaggctc gcctcgctgt cgtcatgagt cgaagttatt
    13501 ttatctacca gcggcgtgtc gtcggtgggg cactgcgtga tgccgggttc gtattcaccc
    13561 tcgcaaacag gacagatcat aagcactccc tttaactgag tttcgcttca aacggtatct
    13621 catcaaaatg tgtcattcgt ttgaaggcgt gaaaacgatc gttgatctca gcgtgcgtca
    13681 gacgcctgac tcgctcggtg ccaaattcct ccacattaaa cgaggccatc accgatccaa
    13741 agatcatggc gcgtctcatt gcgctctcgt cgattgtttc ctggctcgcg agatagccca
    13801 tgaaaccacc tgcgaaagtg tcgccggcac cggtcggatc aaacactgac tcgagcgggt
    13861 acgcgggaat cgcgaaataa ctatcgcgtg tgaagagcgc cgcaccgtac tcgccgcgct
    13921 tgatgatcag tgcttttggt ccccaactga gaatctcgcg cgccgcttta atcaagttgg
    13981 gaatttcggt gagctgtcgc gcctcggcgt cgttgataat cacaacatcg acgactttga
    14041 tcgttttgac gagcgagtcg cgcgtgtgct cgatccagag attcatcgta tcgagcgcga
    14101 cgagctctgc gtcaggcatc tgttccctga cgccgcgttg cagatcaggt tgaatgttac
    14161 cgagaaagag cagacgcgta cgctttgact cttccgagag ctttggttgg aagtctgcaa
    14221 agacgttcaa gtcggtgtag agcgtgtgag caacgttgag atcgtactca taacgaccgc
    14281 cccaccgaaa cgtcttgccg tctgcgattc tttcgatatc gctcgtgccg atgccacggc
    14341 tgtcaaaaac ttcctgctct gcaaagtcac cgcctacgac ggcaacgata ctaaccttcg
    14401 tgaaaaagct ggctgagatc gagaagtgcg acgcggagcc accgagcatc ttgtcgcatt
    14461 taccgaaagg agtttcgacc tcgtcatacg caaccgatcc gacaactaaa agggacatgt
    14521 gtgttttgat ctttgggttg aatttttact gctgctttgg agcttcgagc aggcgcaact
    14581 cttcacgcgc ctcttgggca aacggacttt cgggatagtc tttgatcagc ttgttgagat
    14641 agtcgacgcc catttcgcga aactgtgcgg tcgtcaacgt cttcttttca ctgccgtcga
    14701 aactgacata ctgctccggt cgctgtttac ctttgttctg cgacaaccag agactgctct
    14761 gaccggccat cagcaacact tcatcgatcc tctggaagtt tggattgccg gcgatgatct
    14821 cttcacagcg tttgagtgcg gcgacgtaag cttttttgag tttgaaataa ttctttgcga
    14881 cttcgaggtt gtgaagcgaa tcgcgctcca gatccggatc gcgaagagtc gacggatccg
    14941 gtcccccctt cacgccgcct tgcgcgaacg ttgacggcgc cacaaaaagc acaaaaagga
    15001 tcaaaaacac aaacaggaat ttagatttca tcgtcttacc tcaaatctac gatgcgtatt
    15061 tcttaataat cgcttccaac ttcttcgtcg tcgcttcagg ccagagattg ggagccgtga
    15121 agatcgcgtg ctgcaatgcc gaaccgcact tacaactccg gtcactcgac cgcatcccct
    15181 tcaccgcttc acgcaaaatg atctgcgcgt tccgcacgtt cttgttgaga tactcaacca
    15241 cagtctcgac gctcaccgca tcgtgcccgg gatgccagca atcgtaatcc gttacgagcg
    15301 cgagcgtcgc gtaacagatc tccgcttcgc gcgcgagctt cgcctcctgc aaattcgtca
    15361 tgccgataat gtccatgccc cacgaacgat agacattcga ctccgccaca gtcgagaacg
    15421 ccggcccttc catgcacaaa taggtcccgc cacgatgcgc cttcacgtcc gccgctccac
    15481 acgcctcctg caacacatca cccaaacgcc cacaaaccgg atgtgcaaac gacacgtgcg
    15541 cgacaattcc ttcgccaaag aacgtcgact ctttaacgcg tccccgcgtt cgatcgaaaa
    15601 actgatcggg aatcaccata tcgagcggcg catactgctc ctgcaacgat ccaaccgctg
    15661 aagccgagag aatactctcg accccaagca gcttcatcgc atagatgttt gctcgaaacg
    15721 gaacttctgt cggtgtcaga cgatgaccac gcccatgacg cggcaggaaa gcaacgcgct
    15781 cgccttcgag tgtgccgacg atgaacgcat cggacggtgg tccaaacgga gtgtcaactt
    15841 tgacttcttc gatggcggtc agctccggca tctggtacaa accactgcca ccgataatgc
    15901 cgacgcgaac tcctgctgct gtactcatga aaactcttaa tcgtctcctc gtagtgggtc
    15961 agtttgatgt ccgataagct ttagcttgtc ggaatgtcgc gacaaactaa agtttatcgg
    16021 acacttgcag cgaaagtgaa ctactactgt ctcctcgata cgggatcggg ctgaactcct
    16081 tttcgtgctg ttgtaaccgg cacaaaggct cgtcaggatc gtggtagaac atgcaaatcc
    16141 aattctcgcg cgccgcctgc ggtatcagtc gcttcttcgc gtccaacgtc tcgaccggat
    16201 aaaggtcgta acccatgatc cacggcagac gaacgtgcgc gcgcatcggc acgagatcgg
    16261 cgaaaccaaa catcgtcttt ccgtctcgct ccaaacgcca gcactgcatc gagcgattgt
    16321 ggccagcgat tgtttccatc gtcagaccag gcacgacttc gtaatctgaa tccttcgctt
    16381 cgagctggcc cgactcaacg agtggtcgcc agttctccgg caggtaactc gcacgatcgc
    16441 gctcagtcgg acgatcggca tgttccaact cagcgccgga aacaaagtag cgcgcgtttg
    16501 gaaacgaagc gacgagattg tcttcagcgt caagcttcgt gttgccgccg gcgtgatcga
    16561 aatgcaggtg cgtgttgatg acgatcgtga tctcggctgc agtagcgccg gtatgcgcaa
    16621 agagtgtctc actcagcggt cgcacgcggt cgatgccgta catcgactcc tgcttggcgg
    16681 tccacttgtc gccgattccg gtttcgatca ggatcttttc cgcgcccgta tcgatgaaca
    16741 cacaattcat gttcatgcga atgcggttgt catcgtcggg cgggtgttcc ctggcccaga
    16801 gcttgcgcgg gaccactccg aacatcgcac cgccgtcgag acggaattcg gtgtccggga
    16861 tgatctcgac acgataatca ccgaagttca ttgaaaagca ataggaccta taaatcctat
    16921 aggtcctata aaacgattag tgccccttcg gcttagtcgc gctcggcttt gccggtgatg
    16981 gcgcgggtgc aggagcttcc tgcggcggcg caccaaatcc cggcggcaag gcctgctgcg
    17041 gttgctgttc gggcggcttc accgtatagg tgtcaggcac cttcacgtgc gaacggctca
    17101 cgatctcgtc gagcactttc ttcgccttct cctgctcgac cgcggcct
//
LOCUS       DASSFQ010000121         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1825159, whole genome shotgun sequence.
ACCESSION   DASSFQ010000121 DASSFQ010000000
VERSION     DASSFQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825159"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="rsmH"
                     /locus_tag="VFH96_04115"
     CDS             <1..983
                     /gene="rsmH"
                     /locus_tag="VFH96_04115"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET6973182.1"
                     /translation="VETAAPLGGRGAPHRPVLLEETLQFLAPERGGLFVDGTVGLGGH
                     SEAILKSSQNTRVLGMDLDQAALAYSRERLASFGERFRAFQANFRELNAVLEQANERE
                     PNGILVDLGVSSLQFDSPERGFSFRFDAPLDMRMDSTRGDTAADLLQQLPESEIARII
                     FEYGEERHSRRIARRIVECRDQGKPVTTTAELADLVRQAAGGHKRNQIHPATRTFQAL
                     RIAVNHELEGLGEFVESAVDLLIPNGRFVCLSFHSLEDRILKRELKKLSGHCECPPRL
                     PVCACGARKVVEVLTRKPVAPSDREIDENPRARSAKLRACRKLNDHEEIS"
     gene            967..1311
                     /locus_tag="VFH96_04120"
     CDS             967..1311
                     /locus_tag="VFH96_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973183.1"
                     /translation="MKRFPEHQLNAKIRRSRDTNALSRLALLLFCGLVLAGGFVFAAK
                     QHFAAVQYGYENENLRTERKRLIEENQRLALEKDRAIAPDKLEPAARRLGLKPARPGQ
                     IGNKPRMNADKR"
     gene            1480..3459
                     /locus_tag="VFH96_04125"
     CDS             1480..3459
                     /locus_tag="VFH96_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET6973184.1"
                     /translation="MLVISARLVYLQFSQYDNLANRARQQQQNAFETSPQRGELLDRQ
                     ERQLARSVQTVSLFLDPDGLDAATLDRNAQQLAKSLGLKPADLAKEFRDAAAAKKRFI
                     WIARRLDADVANKVVELKLPGLQTQLEPKRYYPNGSLAAHVLGYVGLDGKGLGGVEQF
                     YNAKITGEAGKLFVEKDAYGKPYESYEIAAKPGQTVVLTIDQTIQYQAEQALQAAVQR
                     SHAKSGTVIVLDPRSGEILALANAPTFDPNNVADAKPETRSNWALQNIYEPGSTFKVV
                     AFSAALEKGLAKVDDRIDCQMGSITVAGRLVHDHKPFGTLTIAEALAKSSNVAAIKLG
                     LRVGDPTMYDYIKRFGFGSRTGVELPGETNGILRKVERWQPSSIGSIAIGQEVGVTPV
                     QMVAAFGALANDGVRIAPHLIREVRDAGGNVVYRAQPEQRRVISAETAIALRGMLEGV
                     TLNGTAKKAQLDGYSAAGKTGTAQKIDPKTKAYSSTKFVGSFVGFAPVSNPQVVIIVV
                     IDEPAGAYHGGDVAAPVFREVAEQVLPTLNVMPDIETTSTDLIAQAPEDPERVAKRQE
                     EQAQSEAQRQATMPTVDSNGGRSGEVVYAVATKKAMLMPDLRGRSVRDVARTCAQLGL
                     QVEARGEGRVLKQNPSAGSEVTTGQLIYVDFGRLQ"
BASE COUNT          817 a          972 c         1045 g          740 t
ORIGIN      
        1 cggtggagac ggctgcgcct ttggggggca gaggcgcgcc ccatcgcccc gtgctgctgg
       61 aagagacatt gcagtttctc gctcctgagc gaggcggtct ctttgtcgat ggcacggttg
      121 gactcggcgg acacagcgag gcgattctga aatcctcgca gaatactcgc gtcctcggaa
      181 tggatctgga ccaagcggcg ctggcttact cccgcgagcg tttagcatct ttcggagaaa
      241 gattccgcgc ctttcaagcc aactttcgcg agctcaacgc tgttctggaa caggcgaatg
      301 agcgcgagcc aaacggcatt ctcgtcgatt tgggcgtctc atcactgcaa tttgattcac
      361 ccgagcgcgg gtttagtttt cgtttcgatg ctcctttaga tatgcggatg gactcgacca
      421 gaggagacac cgcagccgat cttctacagc aacttcccga atcggaaatt gccagaatca
      481 ttttcgaata cggcgaagaa cgacactcac gacgaatcgc ccgtcgcatc gttgaatgtc
      541 gcgatcaagg gaagccggta acgaccaccg cggagttagc ggaccttgtg cggcaagctg
      601 ccggtgggca caagcggaat cagattcatc cggcgactcg cacgttccag gccttgcgaa
      661 tcgccgtcaa tcacgaactc gaagggctgg gagagtttgt cgagtcagca gtcgatcttt
      721 taattcccaa cggcaggttt gtctgtttaa gttttcattc gctcgaggat cgcatcctca
      781 aacgcgaatt gaaaaaactt tcgggtcact gcgaatgtcc accgcgttta ccggtctgcg
      841 catgtggcgc gcgcaaagtt gttgaggtgc tgacacgaaa gcccgtagcg ccgagtgatc
      901 gcgagatcga cgagaacccg cgcgcccgca gcgcaaagct gagagcttgc agaaagctta
      961 acgatcatga agagatttcc tgaacatcaa ctcaacgcaa agattcgccg tagtcgcgat
     1021 acaaacgcgc tctcgcgatt ggcgctgttg ttgttttgtg gattagtact ggctggcgga
     1081 tttgtcttcg ctgccaaaca gcactttgcg gctgtgcagt acggttatga aaatgaaaac
     1141 ttgcgcaccg agcgcaagcg tttaatagag gagaatcaac ggctcgcact cgaaaaagat
     1201 cgcgccattg ctcctgacaa actggaaccc gccgctcggc gtctcgggtt gaaaccggca
     1261 aggcccggac aaatcggaaa taagccgcgg atgaacgccg ataaacgctg atcagcgaaa
     1321 tccgcgttta tccgcgtaac ataaaaagcg tcctcatcac gctactgggt agggggatac
     1381 aagtaggtga tgttggcacg cctcattgct cgatcaaaat ccagaccgga tgtttcacca
     1441 ggccgcgcgc tttttgtcgc ggcttttatt ggtttctgga tgctggtaat ttcggctcgc
     1501 ctggtttacc tccagttctc gcagtacgac aatttggcca accgcgcccg ccagcaacag
     1561 cagaacgcat ttgaaacgag tccccagcgc ggcgagcttc tcgatcgaca ggaacgccaa
     1621 ctcgcccgca gtgtgcagac tgtttccctc tttctcgatc cggacggtct cgatgctgcg
     1681 actctcgatc gcaatgcaca gcaactcgca aagtcgcttg gtttgaaacc agccgacctc
     1741 gcaaaggaat ttcgcgatgc cgccgcagcg aagaaacgat ttatctggat cgcacgtcgt
     1801 ctcgacgcag atgtcgcaaa caaagtcgtc gaactaaaac ttcccggttt gcagacacag
     1861 ctcgagccga aacgttacta cccgaatggt tcactcgccg cgcacgtgct cggctacgtc
     1921 ggactcgacg gcaagggcct gggcggtgtc gaacagtttt acaacgccaa gatcaccggt
     1981 gaagcaggaa agctgtttgt tgaaaaagac gcctacggca agccgtacga gagttatgag
     2041 atcgctgcga agcccggcca gacggttgtg ctgacgatcg atcaaacgat tcagtaccag
     2101 gccgagcagg cattacaagc agcggtgcaa cgttcgcacg cgaaatccgg aacggtgatc
     2161 gtcctcgatc cgcgcagtgg tgagatcctg gcgctcgcaa acgcgccgac gtttgatccg
     2221 aacaacgtgg cggatgcgaa gccggagaca agatcgaact gggcgctaca gaatatttac
     2281 gaaccgggct cgacgttcaa ggtcgtcgct ttctccgccg cgttggagaa ggggctcgcg
     2341 aaagttgacg atcgtatcga ctgccagatg ggctcgatca ctgtcgcggg acgcttggtc
     2401 cacgatcata agccgtttgg aacgttgacg attgccgaag cactcgcgaa gtcgagcaac
     2461 gtcgccgcga tcaagctcgg cctgcgtgtt ggcgacccaa cgatgtacga ctacatcaag
     2521 cgttttggtt ttggttcacg aacgggcgtt gagcttccgg gtgaaacgaa cggcattctg
     2581 agaaaggttg agcgctggca gccgtcgtcg atcggatcga tcgccatcgg tcaggaagtt
     2641 ggtgtgacgc cggtgcagat ggtggctgcg ttcggagcgc ttgctaacga cggcgtcaga
     2701 attgcgccgc atctgatccg cgaagttcgt gatgccggtg gcaatgttgt ctatcgcgcg
     2761 cagccggaac aacgaagagt gatcagtgct gagactgcga tcgctctgcg cggcatgctt
     2821 gaaggtgtga cgttgaacgg aaccgcaaag aaagctcagc ttgacggata ttccgctgcg
     2881 ggcaaaacgg gaacggcgca aaagatcgat ccgaagacga aggcttattc gtcgactaag
     2941 tttgttggat cgttcgtcgg tttcgcgccc gtgagcaatc cacaagtcgt gatcatcgtc
     3001 gtgatcgatg agcccgccgg tgcttatcac ggtggcgacg tcgccgcgcc ggtttttcgt
     3061 gaagtcgccg aacaggtcct gcccacgctc aacgtcatgc ctgacattga aacgacctcg
     3121 acggatctga tcgcgcaggc tcccgaagat cccgagcgtg tcgcgaagcg tcaggaagaa
     3181 caggcgcaat ccgaggcgca gcggcaagcc acaatgccaa ccgtggatag caacggagga
     3241 agaagcggtg aagttgtcta cgctgtagct acgaagaagg cgatgctgat gcctgatttg
     3301 cgcgggcgca gcgtacgcga tgtcgcccgc acttgcgcgc aactcggact gcaagtcgaa
     3361 gcacgcggtg agggacgagt tttgaagcaa aatccgtcag ccggctcgga ggtgaccacc
     3421 gggcagctta tctatgttga ttttgggagg ttgcaataac ttggagtgcg gcgacttgtc
     3481 gccgcctggt acgaatctga gagtttaacc cgacatgaaa atttcacttg agccagccga
     3541 gtcttaccaa agcggcgcca agacgccgca ctcc
//
LOCUS       DASSFQ010000122         8153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_787414, whole genome shotgun sequence.
ACCESSION   DASSFQ010000122 DASSFQ010000000
VERSION     DASSFQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787414"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFH96_04130"
     CDS             complement(<1..593)
                     /locus_tag="VFH96_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HET6973185.1"
                     /translation="MTVLESINSPADLRALSPDQLRTVADEIRDYILETMSRVGGHTG
                     ASLGAVELAVALHYAFNTPEDRLVWDVGHQAYAHKILTGRRDLLPTIKQYGGISGFLR
                     RDESEYDTFGAGHASTSLSAALGMAIARDRKGEDHHVVALIGDASLAGGMAMEAINQA
                     GHLKSRLIVVLNDNEMSIAPAVGALTGYLNRIRGAHG"
     gene            complement(686..1534)
                     /locus_tag="VFH96_04135"
     CDS             complement(686..1534)
                     /locus_tag="VFH96_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6973186.1"
                     /translation="MPKDSFTRRDVLRGLVAAPLLAVSATAAYARLIEPYNYWISETD
                     IFIRDLPQAFAGFRITQLTDIHHSRILGIDEVRRVVNLAQQTKPDMFVLTGDYSTTYR
                     RYIEPCAEALSTLSAPEGVWAVLGNHDHYTDPELTTRALERHHIAVLNNAHTTLQRRS
                     DSLQLSGIDDWSWNATDWERAFSGLKTNAPTILLSHQPAVLDLEQTKNVSLILSGHTH
                     GGQLKFPFLGTPASLFTQDLRYARGLFQRGETQLYVSTGTGVIGLPLRFGVRPEIAVF
                     RLQRAP"
     gene            1685..1775
                     /locus_tag="VFH96_04140"
     tRNA            1685..1775
                     /locus_tag="VFH96_04140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1719..1721,aa:Ser,seq:gct)
     gene            1792..3270
                     /gene="gltX"
                     /locus_tag="VFH96_04145"
     CDS             1792..3270
                     /gene="gltX"
                     /locus_tag="VFH96_04145"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET6973187.1"
                     /translation="MSVRVRFAPSPTGYLHIGAARSALFNWLYARKTGGKFLLRIEDT
                     DLQRSTEESTRSIIEGLEWLGLDYDEDLVFQSANASEHRAAANRLLEEGKAYRDFTPK
                     EQRADASVKEDIAERARANTDQRRNPYRDLPKEESDRRAAAGEPFAVRLKVSSEGQTK
                     FDDIVYGPQERSHSEIEDLVLLRSDGHPLYNLSVVIDDIAMGITHVIRGQDHLTNTHK
                     QVLLYEALSAPVPRFAHLPLILAPNKGKLSKRKHGEVVSLTTYRDRGFVPAAFRNFLA
                     LLGWAPPEGKEVLSKEQLVEYFSLEGIGRANAIFNFQENDPRRWTDDKALWMNAEYIR
                     TMPLDELLPMVKTELRANKLWREEYDDDDSAWFGKTVDLIRQRYFTLKDFSEQGRAYF
                     SDDFDFDEAAVNKNLRKEPRLRELLSGLADKMESVEPFNVETAEAALREFADEAGVKA
                     GLLINGARTMLTGQAVGPSMFEIFDVIGRDASVQRLRSGIPW"
     gene            3293..3904
                     /locus_tag="VFH96_04150"
     CDS             3293..3904
                     /locus_tag="VFH96_04150"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6973188.1"
                     /translation="MLKALALILLTCGVVLGQNSNSSTTTERPQKTNTNRAQTPPAPS
                     PKKAPAPASTAQEPGSEGVLAAFNTLLDGIRHASVAEVMSAYWNNPRLNLFNYNGSVT
                     KGWDQVKKNRESSYPEIKDVKLEVRDVSVTMLGQTGAVVTCQWKQSQTYKGAPETVSG
                     RMTLVFKRIGNAWKAIHLHSSTDTPNPAVIPPSEQPVASPTPE"
     gene            complement(3930..5486)
                     /locus_tag="VFH96_04155"
     CDS             complement(3930..5486)
                     /locus_tag="VFH96_04155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6973189.1"
                     /translation="MSTKTRGSIYALLILIALGFRLFVALRLPNDEPDDGRVYSQIAR
                     NILEQHVYSIENQAPYTPTLIRLPGYPLFVAAIYSVFGHGNNTAVRVAQAVIDTGTCV
                     LIALVAFVWPIHIRRKHRAAFVALALAAVCPFTAIYVATILTEVPTNFLAVAMVLTAT
                     FAFKATTRKRAIMWWIVTGFIAGIEVFFRPDSGLFAAAIGLTLMLPALSGFRKFREAT
                     MYAALFSIAFCLVLVPWTIRNRRLFHVFQPLSPAHAEMPGEFVPRGYLLWVRTWLDDQ
                     RYVAPALWAMDTRQIHVEDFPARAFDSKEERDRVAALFEKYNHPDGDEADEQQSADDQ
                     SDDSSGDEEEQPEEESEPEEHPEPPDANVEMTPEIDAGFAQIGQERVAHSRIRYYVVL
                     PMKRAMTLWFDTHSQYYPFNGELLPLADLDYDIHQQYWLPLFAGLTGIYTLLGVVGGW
                     LLWRSRDPNARRWLLLAALMIFLRLGFFATLENPEPRYTVEFFPFLILLGAVGFSVVL
                     DRITGFTRSS"
     gene            complement(5566..>8153)
                     /locus_tag="VFH96_04160"
     CDS             complement(5566..>8153)
                     /locus_tag="VFH96_04160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973190.1"
                     /translation="YYLILMAYDQHWTGSDAGPVAAQDWFEENLIKRMHDLDSAKTII
                     ALGNYGYNWSDASNTADEVTFQEALINARESSAEPSFDSASRNPYFEYDEEDQSHHKV
                     WFLDGVTAYNQMRAASGYKPAGFGIWRLGSEDPSVWSILGTQNSSPDALRTINYGYEV
                     DFEGNGELLKVMSRPQKGARDLGTDASGFINSEQFTALPSSYVVQRTGDRPGMLALTF
                     DDGPDPRWTPAILDILKRENVPATFFIIGKNGQAYPDLMRRITDEGHEIGNHSFTHPN
                     LGEIPLSLTELELNATQRLIESVTGRSTVLFRPPYFGDAEADKPQEVEPALVAQNLGY
                     VMVGVRIDPDDWQLPVTADQIVQRTIDRATDTNPETRGECILLHDSGGDRSATVEALP
                     RLIHELKARGFRFVPVSELGGFSRDQVMPVIPPNQRVFTRADAIAFFFLSTGSWLLQW
                     IFFIGILLGLARLLFIGSLAVAQWFRSYHRERIHAGSDYMPSVSIIVPAYNEQLVIEN
                     TIHSLLASNYSAYEIIVVDDGSQDATSKVVAEKFAGETRVRLFTVANAGKAAALNTGL
                     RYARGEIVIALDADTLFAPQTVGALAHRFYDKHLGAIAGNAKVGNRVNLVTRWQALEY
                     ITAQNMDRRAFASLNCITVVPGAVGAWRRDLLIEAGGFPSDTLAEDQDLTLRIRRMGY
                     KIGYEETAIAWTEAPHNLRTLAKQRFRWAYGTLQCMWKHRDALLRPRYGALGFVAMPN
                     VWIFQILFPLISPVMDLMLIYVLVSSAIDRWQQGPEYSSTNLQQVILYYALFLAIDWV
                     SAWFAFLLERRERWSLLWWLFLQRFCYRQVMYYVMIKSVAMAIRGPVVGWGKLERKAT
                     AEAGP"
BASE COUNT         1886 a         2356 c         2265 g         1646 t
ORIGIN      
        1 tagccatgcg cgccgcgaat gcggttgaga tagccggtga gcgcgccgac tgcgggcgcg
       61 atcgacatct cgttgtcgtt cagtacgacg atcagacggc tcttcaagtg accggcctgg
      121 ttgatggctt ccatcgccat accacccgcg agtgaagcgt cgccaatcag cgcgacgacg
      181 tggtgatctt cgcctttgcg atcgcgtgcg atggccatgc cgagtgcggc cgagagcgac
      241 gtggaagcgt gtccggctcc gaatgtgtcg tactcagatt cgtcgcggcg caggaaaccg
      301 ctgatgccgc cgtactgttt aatcgtcggg agtaaatcgc ggcgaccggt gaggattttg
      361 tgcgcgtaag cctggtggcc aacgtcccaa accagtcgat cttcgggcgt attgaacgcg
      421 taatgcagcg ctacagcgag ctcgacggca cccagtgacg cgccagtatg gccgccgaca
      481 cgcgacatcg tttcgagtat gtagtcgcga atctcgtcgg cgacggttcg aagttgatct
      541 ggtgaaagag cgcgcaggtc ggctggtgag ttaatactct cgagaactgt catcgtggcc
      601 gcattgtact ccaatcagtg gcattgctcc gattcacttg gtgatataaa gtactctgct
      661 cagttcggcc tgtcaaatta ttttttcacg gcgcgcgttg caatctgaac acggcaatct
      721 cggggcggac gccgaagcgc agcggtagac caatcacgcc tgtccccgtc gagacgtaga
      781 gttgagtctc accacgttga aacaacccgc gcgcgtatct caaatcctgg gtgaataaac
      841 tcgcgggcgt gccgagaaac ggaaacttca gttgtccgcc gtgcgtgtgt ccactcaata
      901 tcagcgatac attcttcgtc tgctcgaggt cgagcactgc cggctgatgc gagagcagga
      961 tcgttggcgc gtttgtcttc aaacccgaga acgcgcgctc ccagtcagtg gcgttccagg
     1021 accaatcgtc tatgcctgat aactgaagcg agtcagaccg gcgctggagc gtcgtgtgtg
     1081 cgttattcag cactgcgatg tgatgacgtt cgagcgcgcg ggtcgtgagc tcgggatcgg
     1141 tatagtgatc gtgattaccg agcacggccc agacgccctc aggcgcactc aatgttgaaa
     1201 gagcttcagc acatggctcg atgtagcgac ggtatgtcgt actgtagtcg ccagtgagca
     1261 cgaacatgtc aggcttcgtc tgttgtgcga gattgaccac gcgtctgact tcatcgatcc
     1321 cgaggatgcg actgtgatga atatcggtga gctgagtgat acgaaagccg gcgaacgcct
     1381 gcggcagatc gcggatgaaa atgtccgtct cagatatcca gtagttgtaa ggctcgatca
     1441 gccgcgcata cgcagcggtc gctgaaaccg cgaggagcgg cgccgcaacc aggccgcgta
     1501 gaacatcacg gcgcgtaaag gaatccttcg gcacgtagag attctactaa aacattagcc
     1561 acggatcttc acggatgaag acggattgct gatccgtctt catccgcgca cttaatccga
     1621 ttaatccgcg tccttgacaa tccgcggctc gcgactagaa ttggtcttcg cctgtttgag
     1681 gcacggagat gtggccgagt ggctgaaggc ggcggtttgc taaaccgtta agggtcaaaa
     1741 gcccttcaca ggttcgaatc ctgtcatctc cgccagttat ttccgctcag aatgtcagtt
     1801 cgcgttcgct tcgcaccatc acccacgggc tacttgcata tcggcgccgc ccgttcggcc
     1861 cttttcaatt ggctctacgc acgaaaaacc ggcggcaagt ttctccttcg catcgaagac
     1921 accgatctcc agcgctcaac tgaagaatca acccgctcga tcatcgaagg tctcgagtgg
     1981 ctcgggctcg actacgacga agatctcgtc ttccagtcgg caaacgcttc cgagcatcgt
     2041 gccgccgcca atcgcctgct cgaagaaggc aaggcgtatc gcgacttcac tccgaaagaa
     2101 caacgcgccg acgcgtcagt gaaagaagac atcgccgaac gcgcgcgcgc caatacggac
     2161 cagcgccgca atccgtatcg cgatctgccg aaagaagaat ccgatcgacg cgccgccgcg
     2221 ggcgagccgt ttgccgtgcg tctgaaagtt tcgtccgaag gccaaactaa attcgacgac
     2281 atcgtctatg gtccgcagga acgcagtcac tcagagatcg aggatctcgt gctgcttcgt
     2341 tccgatggtc atccgctcta caacctctcc gtcgtgatcg acgacatcgc gatgggcatt
     2401 acccacgtga ttcgcggaca ggaccatctc accaacacgc acaagcaggt tttgctctac
     2461 gaagcgctca gcgcgccggt gccgcggttt gcgcacctgc cgctgatcct ggcgccgaac
     2521 aaaggcaagc tctcgaaacg caagcacggc gaggttgttt ccctcacgac gtatcgcgat
     2581 cgcgggtttg tgccggcggc ttttcgcaac tttctcgccc tccttggctg ggcgccgccg
     2641 gaaggaaaag aggtgctcag taaagagcag ttggtggagt atttctcgct cgaaggcatc
     2701 gggcgcgcga atgccatctt caactttcag gagaacgatc cgcgtcgctg gaccgacgac
     2761 aaggcgctgt ggatgaacgc ggaatacatt cgcacgatgc cgctcgacga gttgttgccg
     2821 atggtgaaaa ccgaattgcg cgcgaataaa ctctggcgcg aagagtatga tgacgacgat
     2881 agtgcgtggt ttggcaagac tgtcgacttg attcgccagc gttatttcac cctcaaagat
     2941 ttttcggagc aggggcgcgc atacttctca gatgatttcg acttcgacga ggccgcggtc
     3001 aacaagaacc tgcgcaagga gccgcgcttg cgagagctgc tgagcggtct cgctgataag
     3061 atggaaagtg tcgagccgtt caacgtcgaa acggccgagg cagcgttgcg ggagtttgca
     3121 gacgaagctg gtgtgaaggc tgggttgttg ataaatggag cacgtacgat gcttaccgga
     3181 caggctgttg gaccatcgat gtttgagatc ttcgatgtca tcggccgcga tgcatcagtg
     3241 caaagactaa gaagtgggat accctggtaa tgattagtag aggagtttat tgatgcttaa
     3301 agcgttggct ttgatccttc tgacgtgcgg agttgttctt ggtcaaaata gcaactcatc
     3361 aactactacc gagcggccgc aaaagacaaa cacgaatcga gcgcaaacac cacccgctcc
     3421 ttctccaaag aaggcacccg caccagcatc aacagctcag gaacccggct cggaaggcgt
     3481 actcgctgca ttcaacactc tgctcgacgg catccggcac gcgagcgtcg cagaggtcat
     3541 gagcgcttac tggaacaatc cgcggctcaa cctcttcaac tacaacgggt ccgtgacgaa
     3601 gggttgggac caggttaaga agaatcgcga gagctcgtat cccgagatca aagacgtgaa
     3661 gctcgaggtg cgcgacgtgt cagtgacgat gcttggacaa accggcgcgg ttgttacgtg
     3721 ccagtggaag caatcgcaga cttacaaagg cgcgccggag acggtttcgg gccggatgac
     3781 gctcgtgttc aaacgcatcg gcaatgcctg gaaagcgatt cacctgcact catcaaccga
     3841 cacgcccaac cccgcggtga taccgccgtc cgagcaaccg gtcgcatcgc ccactcccga
     3901 gtaagtcgtt ttacaagatt acaggatttt catgatgatc ttgtaaatcc tgtaatcctg
     3961 tctaaaacta ccgagaaacc cacagcacca agcagaatca gaaacggaaa gaactccacc
     4021 gtatagcgcg gctcagggtt ttcgagcgtc gcaaaaaatc cgagccgtaa aaagatcatc
     4081 agcgccgcga gcagcagcca tcttcgcgca ttaggatctc gcgaacgcca taacaaccag
     4141 ccgccaacca cacccaacag cgtataaata cccgtcaacc cggcaaacag cggcaaccaa
     4201 tactgctggt gaatgtcgta atcgagatct gcaagcggca acaactcacc gttaaacggg
     4261 taatactgcg agtgtgtgtc gaaccaaagc gtcatcgcac gcttcatcgg caacaccacg
     4321 tagtaacgaa tccgtgaatg cgcgacgcgc tcttgcccga tctgcgcgaa gcccgcgtcg
     4381 atctcaggcg tcatctcgac gtttgcatcc ggcggctcgg gatgttcctc cggctccgac
     4441 tcttcttccg gttgctcctc ttcatcacct gacgaatcat cgctctgatc gtcggcggat
     4501 tgttgttcgt cagcttcgtc gccgtccgga tgattgtatt tctcaaacag cgcggcgacg
     4561 cgatcgcgct cttccttcga gtcgaacgcg cgcgcgggaa aatcctcaac atgaatctgt
     4621 cgcgtgtcca tcgcccataa agcaggcgca acgtagcgtt gatcgtcgag ccacgtccgc
     4681 acccatagca gataaccacg cggcacgaac tcacccggca tttccgcgtg ggctggcgag
     4741 agcggctgaa acacgtggaa caggcgtcga ttgcgaatgg tccacggcac gagcacgaga
     4801 cagaaagcta ttgaaaacag agccgcgtac atagtcgctt cacgaaactt cctaaaacct
     4861 gaaagtgctg gaagcatcaa tgtgaggccg atggcggctg caaagagacc gctgtccggg
     4921 cgaaagaaaa cttcaatgcc ggcgatgaac ccagtaacga tccaccacat gatggctcgt
     4981 tttcgcgtcg tcgctttgaa agcaaacgtc gcggtgagca ccatcgcgac ggcgagaaag
     5041 tttgtcggca cttcggtgag gattgtcgcg acatagatcg ctgtgaacgg acacacagcg
     5101 gcgagtgcaa gcgcgacgaa tgctgcccga tgcttcctcc taatatgaat gggccacaca
     5161 aaagcaacga gcgcaatcag cacacacgta ccggtgtcaa tcaccgcttg cgcaacgcgc
     5221 acggccgtat tgttgccatg accgaagact gaataaatcg cggcgacgaa gagcggataa
     5281 ccgggaagac ggatcaacgt aggcgtgtaa ggcgcctggt tctcgatcga gtagacgtgc
     5341 tgttccagga tgttgcgcgc gatctgcgaa tagacgcgcc cgtcatccgg ctcgtcgttt
     5401 ggcagtcgca acgcgacaaa tagccgaaag ccaagcgcga taagaattaa gagtgcgtaa
     5461 atggagccgc gcgtcttggt ggacatgtca gttgtagaac aggatttaca agattttcag
     5521 gattaacatg taaattctgg aaatctgtca atcctgtcta acggcttatg gcccagcctc
     5581 ggccgtcgcc ttcctttcta gcttgcccca tccaaccacc ggcccacgaa tcgccatcgc
     5641 caccgactta atcatcacgt aatacatcac ctgccggtaa cagaaccgct gcaggaacaa
     5701 ccaccacaac aaactccaac gctcacgtcg ctccagcaaa aacgcaaacc acgccgacac
     5761 ccaatcgatc gcgagaaaca acgcgtaata aagaatcacc tgctgtaagt ttgtcgacga
     5821 atactcaggc ccttgttgcc agcgatcgat cgcgctcgac acgagcacgt aaatcaacat
     5881 cagatccatc accggcgaga tcagcggaaa cagaatctga aagatccaaa cattgggcat
     5941 cgccacaaag ccgagcgcgc cataacgcgg ccgcaacagc gcgtcgcgat gcttccacat
     6001 gcactgcaac gtcccatacg cccagcgaaa ccgctgcttg gcaagggtcc gaagattgtg
     6061 cggcgcttca gtccaggcga tcgcggtttc ttcgtaacca atcttgtaac ccatgcgcct
     6121 gattcgcagc gtcagatcct gatcttctgc cagcgtgtct gacggaaaac caccagcttc
     6181 gatcaagaga tccctgcgcc acgcaccgac tgcaccaggc acgaccgtga tgcaattcaa
     6241 actcgcaaac gcgcggcggt ccatgttctg cgcggtgatg tattcgagcg cctgccaacg
     6301 cgtgacgaga ttcacgcgat tgccaacctt cgcattcccc gcaatcgcgc cgaggtgctt
     6361 gtcgtaaaac ctgtgcgcga gcgcgccgac ggtctgcggc gcaaacaacg tgtcagcatc
     6421 gagtgcgatg acgatctcgc cgcgcgcgta acgcagaccc gtattgagcg ccgccgcctt
     6481 gcccgcattc gcaactgtga acaacctcac gcgcgtttcg cctgcgaatt tttcagcgac
     6541 gactttgctc gtcgcatcct gcgagccgtc atctacgacg atgatctcgt aagcggaata
     6601 gttagaagca agcagactgt ggatcgtgtt ttcgatcacg agctgctcgt tgtacgcggg
     6661 cacgatgatc gaaacggacg gcatgtaatc gctgccggcg tgaatgcgct cgcgatggta
     6721 cgatctgaac cactgtgcaa ctgccagcga gccgataaaa agcaatcgcg ccagaccgag
     6781 caggattccg ataaagaaga tccattgcaa caaccaactg ccggtcgaca aaaagaaaaa
     6841 agcgatcgca tcggcgcgcg tgaagacgcg ttggttcggc ggaatcaccg gcatcacctg
     6901 atcccgcgag aatccgccga gctctgaaac cggaacaaac cgaaacccgc gcgctttcaa
     6961 ctcgtgaatc aagcgcggca acgcttccac tgttgccgaa cgatccccac ccgagtcgtg
     7021 cagcagaatg cattcgccgc gtgtttcagg attcgtgtct gtcgcgcgat cgatcgtgcg
     7081 ctggacgatc tgatccgccg ttacgggaag ttgccagtcg tcaggatcga tacgcacacc
     7141 aaccatcacg tagccgaggt tctgcgcaac gagcgcgggc tcaacttcct gcggcttatc
     7201 tgcttccgcg tcgccgaaat agggtggacg aaacagcact gtcgatcgac ctgttaccga
     7261 ctcgatcaaa cgctgcgtcg cgttcaattc gagctcagtc aacgacaacg gaatttcgcc
     7321 gaggttcgga tgcgtgaacg agtggttacc gatctcgtga ccctcgtcag taatgcgtcg
     7381 catcagatcc ggatacgcct ggccgttctt gccgatgatg aagaacgttg cgggaacgtt
     7441 ttcacgtttg aggatgtcga gaatcgcagg tgtccagcgt ggatcaggac catcgtcaaa
     7501 cgtcagcgcg agcatgccgg gacgatcgcc tgttcgttgc acgacgtacg acgacggcaa
     7561 cgcagtgaat tgttccgagt tgataaagcc gctcgcgtcc gtccccagat cgcgcgcgcc
     7621 cttctgtgga cgcgacatca ctttcaacag ctcgccattg ccttcgaagt cgacttcgta
     7681 accgtagttg atcgtgcgca gcgcgtcagg cgagctgttc tgtgtgccga ggatggacca
     7741 cacggacgga tcttcagatc caagccgcca gattccgaaa cccgccggtt tgtaaccgct
     7801 cgcggctcgc atctggttgt aagccgtcac accgtcgaga aaccagacct tgtgatgcga
     7861 ctgatcttct tcgtcgtatt cgaagtaagg attgcgcgag gcggaatcga aactcggctc
     7921 ggcgctcgac tcgcgcgcgt tgatcagcgc ttcctgaaat gtgacttcgt cggcagtgtt
     7981 gctggcgtcg ctccagttgt agccatagtt gccgagcgcg atgatggtct tggcggagtc
     8041 gaggtcgtgc atgcgcttga tgaggttttc ttcgaaccag tcctgcgcgg cgaccgggcc
     8101 tgcgtcgctg ccggtccagt gctgatcata agccatcagg atcaggtaat agg
//
LOCUS       DASSFQ010000123         5689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1689332, whole genome shotgun sequence.
ACCESSION   DASSFQ010000123 DASSFQ010000000
VERSION     DASSFQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689332"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2654)
                     /locus_tag="VFH96_04165"
     CDS             complement(<1..2654)
                     /locus_tag="VFH96_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973191.1"
                     /translation="MPDTISHYPEEQLVRYRAISIGLAAFAFAIGGAALLGWVFDNEF
                     LKQIHPALVNMKANTAVCLMLVAASCFLINDRSPSPGKRFVRQLFAAIVALVGLVTLS
                     EHIFGWNTGLDQVLFHETWEQSGLSFPGRMGVAASLNFVFLGVALSFLDVKARRWFRV
                     SNIAVTLVVTVTLLVFLSYFYGIERVEPIALYFTIALHTVIAFLCLCASILLARPERG
                     MVATMLGNSPGAMVARRMLPAFLVVILLGWIRTLTRNSEWFSPNFTTAAFVVAVLLLL
                     AVLIWLTALSLNRTDRERRVAELALRDSEARLTALLEQLPVGIGLTDRDGRFLIRNSL
                     LNNFVGDRLSSLDPVFAARWRAWDEEGRQLDPSEWPSARALRGESVSPGVEMLYTGAG
                     GKQIWTRVLSVPFRDQTDELSGVIVVVQDIDIQRRAENRLDVLVRVSELIRTIEDPYE
                     LSYAVAETVGNHLNVRRCLFNETDIEHDIEIIHRDYTDRAESVAGQHRVSEYSSITTQ
                     DMQLGKTVVNYDSKTDPRTAADYNHIYEPNGERAYVAVPLMREGRWVASFWSSDDQPR
                     QWTKEEVALLQTVAERTWTAIEKLRAEAERERLLMNVQEARDAAEKANQLKDEFLATL
                     SHELRNPLNVILGYSELLLRMREIEASPRLVQMGEALRRNAQSQSQLINDLLDLSRLQ
                     RGKISLNQETVSLPAIIDNAVETVRADAAAKGIDIRVNVGDQLLLVEGDRLRLQQIAW
                     NLLNNAVKFTPAGGSIEIALSNENDRAMFVVTDTGQGIDANFLPHVFEMFRQADGSNR
                     RRHGGLGIGLALVNQLVQLHGGTINAASDGPNKGARFTVRLPLLPEAARPATPASSRV
                     ELNVFAQRNFLIVDDSEDTIAMLNELLKV"
     gene            complement(2898..4898)
                     /locus_tag="VFH96_04170"
     CDS             complement(2898..4898)
                     /locus_tag="VFH96_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6973192.1"
                     /translation="MATAIKRLLVGRALRTEQAAHERLTKKTALAVFSSDALSSTAYA
                     TEEILLVLAVAAAATNGGSFSYVVPVSVGIAVLLAIVATSYRQTIHAYPSGGGAYIVA
                     KENLGQTPGLIAGAALLVDYVLTVSVSIAAGVAAITSAVQGTHYAWINDHKVILCLVF
                     IAFIAIANLRGVRESGALFATPTYVFVVSFLFMIVFGLFHYFIYGGAAAIPTQEELKT
                     AEGYHLQPLTLFLLLGAFSNGCAALTGIEAISNGVQAFKKPEARNAATTLIWMAILLT
                     AMFLGTSVMAYLYHVHPKQSETVISQFARIMFTGPFGWFYYVVQAATAAILVLAANTA
                     FADFPRLSSLLARDRFLPRQFANRGDRLVFSNGIVILAIFSGVLVIAFGGDTSRLIPL
                     YAVGVFLSFTLSQSGMVVHWLRERAKLRLQNAAPPEILPTSNGPLPDVALQEEEIHRS
                     YFVTDEVTAPANWKKSLAINAVGALATFVVLCVFIATKFIHGAWIVVVVIPLLVLLFR
                     AIHNHYVMVAKQLSTEGLEQIHPIKHTVIVPISGIHRGVVNALQYARSIASDKVQAVY
                     VDFEEEATASLKDKWDRWGAGVQLVVLPSPYRELTRPLLRHIHRLKKENSDDIITIVV
                     PEFIPARWWQHFLHNQSSLLLKGSLLFKKGVIVTSIPYHLEH"
     gene            complement(4900..>5689)
                     /locus_tag="VFH96_04175"
     CDS             complement(4900..>5689)
                     /locus_tag="VFH96_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973193.1"
                     /translation="NEIDPALPRSVLLFMSNRILLIDDEEHIRQMMRLTLEAAGYEVG
                     EASNGEDGLRLYADGSTWNAVVLDQRMPGIDGLETLRRLKTQTSHACVVMATAYASIE
                     LAVDAMKLGATDFVRKPMTPKVLRNAVAAALAKSNVEVHSDKPKSVIRTITMNGFTIL
                     DDEESPVLDVLLRRFIVVSPEGEKHEVVVQIELEPIEYVERMTGRRLPPESSFWTVEA
                     RRLLSDFLWNERNVPSTRRLILSNLDPDKLPNAARWQTEEITTK"
BASE COUNT         1334 a         1628 c         1567 g         1160 t
ORIGIN      
        1 actttcaata gctcgttgag catcgcgatc gtatcttcag aatcatcgac gatcagaaaa
       61 ttcctctgcg cgaacacgtt caactcaacc cgggacgaag ctggcgtcgc tggtctcgct
      121 gcctcgggca acagcggcag acgcacggtg aatcgcgcgc ctttgtttgg accatcagat
      181 gccgcattga tcgttccgcc gtgaagctgc acaagctgat tcacgagggc gaggccgata
      241 ccgaggccgc catgtcgtct tcgattgctg ccgtcagcct ggcgaaacat ctcgaaaaca
      301 tgcggcagaa aattcgcatc aattccctgc cccgtatccg tcacgacaaa catcgcgcgg
      361 tcgttttcat tgctcaacgc gatctcgatg ctgccgcccg cgggcgtaaa cttcaccgca
      421 ttgttcaata aattccaggc aatctgctgc aatcgcaaac gatcaccttc gacgagcaaa
      481 agctgatcgc cgacgttcac acgaatgtcg atccccttcg ccgctgcgtc tgcacgaaca
      541 gtctcgacgg cgttatcgat gatcgccggt aacgacacgg tctcctgatt caaagagatc
      601 ttgccgcgct gcaagcgcga aagatcgagc agatcgttaa tcaactgtga ctgcgattgg
      661 gcgttgcgtc tgagggcttc acccatctgg accaaacgcg gcgacgcttc gatctcgcgc
      721 attcgtaaca gcaactccga gtaaccgaga atcacgttca gcggattgcg cagctcgtgc
      781 gagagcgtcg ccaggaattc atccttgagt tgattcgctt tctctgccgc gtcgcgcgct
      841 tcctgcacgt tcatcaacaa acgttcgcgc tcagcttcag cacgcagctt ctcgatcgcg
      901 gtccaggtcc gctcagccac agtttgcagt agcgccactt cctccttcgt ccattgacgc
      961 ggttggtcgt cactggacca gaacgaagcc acccaacgtc cctcacgcat caagggcacc
     1021 gcgacgtaag cacgctcgcc attcggctcg tagatgtgat tgtagtcggc cgccgtgcgt
     1081 ggatcggttt tcgagtcata gttgacgaca gtcttgccca gctgcatgtc ctgcgtcgta
     1141 atgctgctgt actcggagac gcgatgctgg ccggccaccg attcagcacg gtcggtgtag
     1201 tcgcgatgaa tgatctcgat atcgtgctcg atgtcagtct cattgaacaa acaacggcgc
     1261 acgttgaggt gattgccaac agtttccgcg actgcgtatg acaactcgta tggatcctcg
     1321 atcgtgcgta tcagctcgct aacgcgcacg agcacatcga ggcggttttc agcgcgtcgt
     1381 tgtatatcga tgtcctgaac gacgacgatg acgccgctca gctcatccgt ctgatcgcga
     1441 aatggaacgc tgagtacgcg cgtccagatc tgcttgccac ccgcgccggt atagagcatc
     1501 tcgacgccgg gacttactga ctcacctcgc aatgcgcgcg cgctcggcca ttcagacgga
     1561 tcgagttgac ggccttcttc gtcccacgca cgccagcgcg cagcaaacac aggatcgaga
     1621 gacgagagtc gatcgccgac gaagttattt aaaagtgaat tgcggatcag gaaacgtccg
     1681 tcacgatcgg tgagtccgat gccgacgggg agttgttcga ggagtgcggt caaacgcgct
     1741 tcgctgtctc gcagcgccag ctcggcgacg cgtctttctc tgtcggtgcg attgagtgat
     1801 aacgcagtca gccagatcag gacggcgagc agcagcagca ctgctacgac gaacgcggcg
     1861 gtggtgaagt taggactgaa ccattctgaa ttccgcgtta gtgttctgat ccagcctaac
     1921 aggattacaa ccagaaacgc cggcagcatg cgtcgcgcga ccatcgcgcc gggactattt
     1981 cccagcatcg tggcgaccat gccgcgctcc gggcgcgcca gcaggatcga ggcgcacaaa
     2041 cagaggaacg cgatcaccgt gtgtaaagcg atcgtgaaat acagcgcgat cggctcaacc
     2101 cgctcgatgc cataaaagta ggaaagaaag acgagcagcg tgacggttac tacgagggtc
     2161 actgcgatgt ttgaaacgcg aaaccatcgc cttgccttca catcgagaaa cgaaagcgcg
     2221 acgccgagaa agacgaagtt gagcgacgcc gccacaccca tgcgaccggg aaacgagagg
     2281 cctgactgtt cccacgtttc gtgaaacagc acctgatcga ggccagtgtt ccagccgaag
     2341 atgtgttcgc tcaacgtgac taagccgacg agggcgacga tcgcggcaaa tagttggcga
     2401 acgaagcgtt tgcctggaga cggtgagcgg tcgttaatca gaaagcagga cgcggcgacg
     2461 agcatgaggc acaccgcggt gtttgccttc atgttgacga gcgcgggatg gatttgtttg
     2521 aggaactcat tatcaaaaac ccacccgagc aacgccgcgc cgccgatagc aaacgcgaac
     2581 gccgcgaggc caatcgagat cgcccggtaa cgcaccagtt gttcttcggg atagtgtgag
     2641 atggtatccg gcattaaact tttaccaggt actttagctc ttaaaaattc gaaacagata
     2701 gtaatacgac gggaacgaca aaatcacgcc ggcaatgagt gcggcgagca gcagttaaga
     2761 agagttgcca caaaaaggca caaaaggcac aaagtaaaag gttcagagtt caagctttag
     2821 cttgcggcct aaaagctgag actgcagcct aaagtgtgaa ctctgaacct tttactttgt
     2881 gcctctttgt ggctgaatta atgttcgagg tgatatggga tactcgtgac gatcacgccc
     2941 ttcttgaaga gcagcgagcc tttgagcagc agcgagcttt ggttgtgcag aaagtgttgc
     3001 caccagcgcg ccgggatgaa ttcgggcacc acgatcgtga tgatatcgtc ggagttttct
     3061 ttctttagac ggtgaatgtg tcgcaacaac gggcgcgtga gctcgcgata cggcgaaggc
     3121 agtaccacga gctgcacacc agcgccccag cggtcccatt tatctttcaa actcgccgtc
     3181 gcttcctcct cgaaatcaac atacacggcc tgcactttgt cagacgcgat cgagcgcgcg
     3241 tactgcaacg cgttcacaac gccgcgatgg atgccggaga tcggaacgat gacggtatgt
     3301 ttgatcgggt gtatctgttc gagtccttca gtcgagagct gctttgcgac catcacgtag
     3361 tggttgtgaa tcgctctgaa gagcagcacg agcagcggaa tcacaaccac tacgatccac
     3421 gcgccgtgaa taaacttcgt ggcgatgaaa acgcagagca cgacaaaagt cgcgagtgcg
     3481 ccgacggcgt tgatcgccag cgacttcttc cagtttgcgg gtgctgttac ttcgtcagtg
     3541 acgaagtacg accggtgtat ctcttcctcc tgcaaggcaa cgtccggcaa tggaccatta
     3601 ctcgttggca agatttccgg aggcgcggcg ttctgcaaac gcagcttcgc tcgctcacgc
     3661 aaccagtgta cgaccatccc tgactgcgac aatgtgaacg acagaaacac gccgacagcg
     3721 tagagcggga tcaaacgcga agtatcaccc ccaaaagcaa tcacgaggac gcccgagaag
     3781 atcgcgagaa tcacgatccc gttcgagaac accaaacggt cgccacgatt cgcaaactgc
     3841 cgcggcagaa aacgatcgcg cgccagcagg ctcgagagac gtggaaagtc tgcaaacgca
     3901 gtattcgcgg cgagcacgag gattgcagcc gtcgccgcct ggaccacgta gtaaaaccat
     3961 ccgaatggcc cggtgaacat gatgcgcgca aactgcgaga tcacggtctc gctctgtttt
     4021 ggatgcacgt gatacaggta agccatcacg ctcgtcccga gaaacatcgc ggtcaggagt
     4081 atggccatcc agatcaacgt cgtcgccgca tttcgcgctt cgggtttttt gaatgcctgc
     4141 acaccattcg agatcgcttc gataccggtc aatgcagcgc agccgtttga aaacgcgccg
     4201 agcagtagaa acaacgtcaa cggctgcaag tgataacctt cggcagtctt caattcttcc
     4261 tgcgtcggaa ttgccgcggc gccgccgtag atgaagtaat gaaacaatcc gaagacgatc
     4321 atgaagagaa agctgaccac aaagacgtag gtgggcgtgg cgaagagtgc gccggattca
     4381 cgcacgccac gcaagttcgc gatcgcgatg aaggcgatga acacgagaca gagaataact
     4441 ttgtgatcgt tgatccatgc gtaatgtgtg ccctgcactg ctgaggtgat cgcagccaca
     4501 ccggcagcga tcgacactga cactgtcaaa acgtaatcca ccagcagcgc cgcaccggca
     4561 atcaagccag gcgtttgacc gagattttct ttcgcgacga tataggcgcc gccgcctgac
     4621 ggatacgcat ggatcgtctg tcgataactc gtcgcgacga tcgccagcaa gacagcgatc
     4681 ccgaccgaga ccggaaccac atagctgaac gatccgccat tcgtcgcagc cgcagcgacc
     4741 gcaagcacga gcaggatttc ttcagtcgca tacgccgtcg aggaaagcgc gtccgatgag
     4801 aaaacggcga gggcggtctt cttcgtgagg cgctcgtgtg ccgcctgttc agttcgcagt
     4861 gcccggccga caagtaaccg ctttatcgca gtcgccatgt tatttcgttg ttatctcctc
     4921 tgtttgccaa cgagccgcat tgggaagttt gtcgggatcg agattgctca aaatgagtcg
     4981 tcgcgttgac ggcacgttgc gttcgttcca taaaaaatcg ctcaggaggc ggcgcgcctc
     5041 tacggtccag aacgagctct cgggcggcag acgccggccc gtcatgcgct cgacgtattc
     5101 gatcggttcc aactcgatct ggaccacgac ttcgtgcttc tcgccttcag ggctgacgac
     5161 gatgaaacgc cgtagcagca cgtcgagcac tggtgactct tcatcgtcga gaatcgtgaa
     5221 accgttcatc gtgatcgtcc ggatcactga cttaggcttg tccgaatgaa cttccacatt
     5281 cgatttcgcc aacgctgcgg cgactgcgtt acgcaagact ttcggagtca ttggcttgcg
     5341 cacgaagtcc gtcgcgccaa gcttcatcgc gtcaacggca agctcgatcg atgcgtaggc
     5401 agtggccatc actacacacg cgtgggaagt ttgggttttg aggcggcgga gcgtttccag
     5461 gccatcgatt ccgggcattc tctgatctag aacaacggca ttccacgttg agccatcagc
     5521 gtagagacgc aagccgtcct caccgttgct cgcctcacca acctcataac cagccgcctc
     5581 gagcgtcaac cgcatcattt ggcggatgtg ttcttcatcg tcaattaata gaattcgatt
     5641 gctcataaat aacagaaccg agcgcggtag cgccgggtca atctcattc
//
LOCUS       DASSFQ010000124        33104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1689658, whole genome shotgun sequence.
ACCESSION   DASSFQ010000124 DASSFQ010000000
VERSION     DASSFQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 33104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33104
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689658"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VFH96_04180"
     CDS             <1..1446
                     /locus_tag="VFH96_04180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6973194.1"
                     /translation="VDFPYPVIPIVRHHHEQWDGRGYPDRLRGEQIPITARIISVVDC
                     FDSIREDRPFRRGMTLDEATALLLRGSGIHFDPVVVEQFIKHLPRFDAEIERLGLQHQ
                     CANYSTEPPIQLSEVDMSQTRERGSYIAYDQIKKAHREVYALYEIARTFGTSLNVEHT
                     LEILVDKVGHVVPFDTCIVYFYDDQKGYATARHVAGKHVQKLAGRCIALGEGVTGFAL
                     ANRSAVNQLHPSLDFTDINPEAGVKYRSMASLPLFKDDVLLGALSVYSAELEQYTDDH
                     MRLLETVTRLASDALGNAMQHAEAESNALTDPLTGLPNARYLSLRFEEEAARARRTDR
                     TFQVVMLDLDEFKNVNDTYGHKAGDKMLREVAHLIAGQLREYDFLARSGGDEFVALVQ
                     EVVGSQVEDLCMRIESAVSKFSLPLGRNRSARVGISIGTATFGVDGDTLDQLVVAADN
                     EMYRMKSTHRSTRIVEEPIDTDLQNSSAPAC"
     gene            1637..7195
                     /locus_tag="VFH96_04185"
     CDS             1637..7195
                     /locus_tag="VFH96_04185"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HET6973195.1"
                     /translation="MSRRVVPRLTRRIAIALTLCIALLPAHHIWLLATEAAQGHPEES
                     EQKTKPKPGKPEGMLPNLEDVRKEKQLERPSPPPIPSTIRSKRNTGKPWDGRRVGDPW
                     PEGTSNQAVNNADSISPMRSQRTRRAHARMRVNPSPLAWDDQFVQNFFSWTLARSSFS
                     DENTYWYDHLRVAYGQGQEALKLAAIEFGRTLFESAEYDARNRSNHWYVYDLYKAYLM
                     RDPDPGGWATWEAVVATNGREYGRRGFEESGEFATLIANILPNGSATSNAASLISARV
                     DARNQPGNGMLTRDATWSIPLLSLPGRAGLDLGLGLSYSSMSWTRSGPYIYFDEDNGF
                     PSPGFRLGFPIVQQKTFDAQTARKAYLLITAAGKRVELRQVGSSNIYDAADSSYLRLT
                     DNGSLRVQSTDGTKLTLTYFNGEFHCTEVKDSNGNYITINYNSLGQLTNITDTLARVI
                     TFNYDGNANLLSITQSWAGQPSHQWVSFGWSTRAMQSSFSDASLRGVIGTANGKSVPV
                     ITQVGLNDTSHFTFDYNNSLQVSWLKNYFDTLERNATTFTYETSAGDAPRLLDSRVSA
                     LNWTGINNVPAQVITQYGVAPDGACTMTAPDGTIYKEYYGTGWQKGLTTLSEIWSGGV
                     RKKWITTAWTQDDTNLSYPKNPRPYDTSIYDEAGNRRRVETIYTSYNLPDAVALPTEV
                     KEYKANGTDVWRRTTTMYYDGGQPYIDRRVLGLVREVMVYDENNQPLSRVWYDYDWNT
                     PDAWVATPQPAPQHDPSGDARGRGNLAWMGRWDVNDFDNFDKNSKSYVKYNRTGSVIK
                     TEDHFGHGKTISYTDSFSDGVTTRYTYAYPTTITDSDGGSSYVQYNVDTGATTRTESP
                     APAGQTQGPIQTMTYNNLGQLERLTTANNSAYTRYYYGPYFIQSYASVNNIADDAYTN
                     RVFDGLGRVRAVASNHPDSAGGYKAQLTVYDSMGRAAQTSNPAEITELWAPYGDDNAG
                     WLYTQQSYDWQGRPRITTNTDLSTNEISYEGCSCAGSDVITTTDAVARKTKSYRDVLG
                     REWKSEILNTNGTVYATTINAYNGRDQILSSKQYVGLATSDGSCPVNTCQETTYGYDG
                     HGRLSSRHLPEQASGTSTVYTYRPDDTLWTETDSRGATTTYTYNGRDLPITITYTASG
                     ISVPSPTTIEYDAAGNRSSMADSMGTVSYDYDQLSRLKHEKRYFSNLPNAPVPEHKYT
                     ITYDYNLAGNLTSITDPFGDSFSYTRNAVGQLKTVSGSPYAGWTSYVTNVTYRAWGAP
                     KGVSYVGGSSTIWFNSRMQPSAFTSTAAGMNESYSYYADGRLHTLTDLNDTAGSNPPV
                     TLRFLSRSYNYDHVGRVTNGFGTGSGAPGVPFTQTYSYDAFGNMTGRTGSYYNYQSDS
                     SLTKTDTSTYVNNRRTSWGYDLEGQVTSTPLTSTDSPRTMAYDAVGRMVSSVETGALN
                     TTTYTCAYDGDDQMVFESSTTSPGTSESSYLVRSSVLGDVLTRLDQSGNKKITYVPAE
                     GLLFATQRASGGPGPFVLPTYRNPLGISETTKAVYDPLGNYIPFEAHDDPRPPAGSYS
                     SASMSGLSSSQANAESYGVGCLMDNVPTTCKKVMDHLEKERAQKLQVIGPASTSFITR
                     LLMSVTAGTAWVPVGPKKLIQVFELKNGKALKIYSQAGKLWTYYIVAPGSPDRFKPNT
                     ISKQPDTVTDNPPSIDNSKAKDQACQIEVSFTGSGSRGLQNGPSTMNLNNRGQVFGIG
                     FTVNISGLSGPINIVSEAKDRRPNAAWNVEQYVADFNKSNGVVVRQDGGARLEALGRA
                     APVVDGGTVEWWDHPGTTVSGNSQYFTKRNFYIKAYKGKQHCEVSFHLTFRLFFGKPQ
                     DTGWGDGPYP"
     gene            7208..7924
                     /locus_tag="VFH96_04190"
     CDS             7208..7924
                     /locus_tag="VFH96_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6973196.1"
                     /translation="MGTFKVIGQFGFLPIFVLLVVFTTRVDPQHSANASRRVRQDSEC
                     LSPGVLTNALKRLSESYDEARGAHDTLIKAAGKSARCKQQVVLSLMQAMDKPGLDIRR
                     NQGDYYLWREGATLLGELKATEALDLLISHMGMSDGEWSVSMVHQPALSGIIEMGPIA
                     IPKLGVVLGSQDTATRHDAVYCIAQIGGPAALRVLKQALPSESDKCIQRFIQASLKSL
                     DNEKNKLKDNGEWLPAFLCN"
     gene            8350..10398
                     /locus_tag="VFH96_04195"
     CDS             8350..10398
                     /locus_tag="VFH96_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973197.1"
                     /translation="MVAPAENHSATLLIVDDDAAARDRFREIFEAAQYRVVTANEAAS
                     ALRVLKGVRCDLVALDLEMPGVDGLALCKLLRAQPATSKLPIIALSESDDEARKVQAF
                     TAGADDYITKPSTPGELLSRVSLHVRTSQREWALLGSNHELRFLSDIGRGLLRALEPE
                     QLVRRVAGATYEAIESSLCAAYVTVGKDKDAMCVFDREGAADDPTLLNTEALQEWLRS
                     AESANSLLITEKSSFLLRDDVHAAEYVSPFRFAGRAKGALIVAFDRREECGETERRLI
                     EAAAQQTSLAAHICSLYQSARDTSVSLAREVERRTAESEAQARFIEAIVDSLPLSLYA
                     IDREYRVVAWNRNRELGELGIPRGSVLGKNVFDVLTRQPRDMLAEEFARVFESGEIER
                     IEQETPSPKGDIRHWVISKIPMWADNTGEVSHVITVGEEVTDRVEANRAIARAEKLAA
                     VGRLAAGVVHEINNPLATISACAESLEARVNEGAFEGSAAALQDLREYLGLIRSEAFR
                     CKSITMGLLDFSRTRTSDHVTINLADVIQSAVRLLSHQKRSPFVDFKTEIAKDLQFVS
                     GDPGQLQQAVIALATNALDAMGDSGTLTIVSRNEGDKVVVDVNDTGVGIPPENLPKIF
                     EPFFTTKEIGRGTGLGLAVCYGILTEHGGTLDVQSTVGSGTTFTITLSAVKISEEQTV
                     "
     gene            10395..11816
                     /locus_tag="VFH96_04200"
     CDS             10395..11816
                     /locus_tag="VFH96_04200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6973198.1"
                     /translation="MSEQQQQARILIAEDEANLRLVLQKELERLGYRVQAAPDGEAAL
                     RKLEESNVDVLLCDINMPRMDGMELLRRVYQRPNPPEVIMLTGQATVETAVEAMKLGA
                     YDYLTKPYSITELDVRVKQAADKRRLRVDNVRLREQLARQSALPEIVSVSEVMKEAIR
                     LIERVAPSEASVLITGESGTGKELVAHAIHRLSNRAEASFVDLNCAAFQESLLESELF
                     GYEAGAFSGAKGRKLGLIELADGGTLFLDEVTELPAQLQAKLLRAIETRTFFRVGGVR
                     KVEVNVRIVAATNRNLDTVVNDGVFRSDLLYRINGFQISLPPLRERPEDIDPLARYLL
                     KQIAGTQPPELTEDAFTALRMYHWPGNVRQLKNCLERAVILSNNGRITLSELPPEVTR
                     PAVPAPALTSVAPAVVGGEVVTIPAAAIPSSTGSLREVERQQILAALEQTGWHRGKTA
                     EILGISPSTLYRRLRDYDLERRR"
     gene            11838..12809
                     /locus_tag="VFH96_04205"
     CDS             11838..12809
                     /locus_tag="VFH96_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6973199.1"
                     /translation="MRIRWGLIGCGDISRRRVAPALRDSPLCELVAVSRAKAELAESF
                     AKEFGARRWCADWHDFLMDYEIDAIYVATPVHLHAEQVIAAAERGKHVLCEKPMALNV
                     AECDRMIDACRSNDVKLGVAYYRHFYPVVRRMKELIDSGELGVPVVAQVNAFEWFDPE
                     PGSARSWLLRKELSGGGPMFDFGCHRIEVLLDLLGDISDVKATLANAFFNREVEDTAT
                     AMFRFERGTCGTLTVTHAARQPQDTLDLFFTNGSIHIPVLNEGTMRVLTKDGERIETH
                     PNPSNIHQPLIDDFARAVLENRDPLVTGEIGRRVASTEQKIYNQSAF"
     gene            complement(12820..14430)
                     /locus_tag="VFH96_04210"
     CDS             complement(12820..14430)
                     /locus_tag="VFH96_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET6973200.1"
                     /translation="MADYFLYHNVEEGRENKVCLYYEDRQWTYGEVARLSNQTGNALR
                     ELGVNIEDRVLMVLPDCPEFVWTWFGAARIGAVITMVNPLLPVADYEYYLEYTRAKVA
                     IVHESLLDTFAEAATSARYLRSVLVVGTGGHGGPPLQLNPEGHLDRDESTVGVALRGH
                     PFPENTRWIEFHEVVDAASDECQPADTHRDDIAIWLFTSGSTGHPKGAVHLQHDLPFN
                     TEVFAKATMGVCADDLTVSVPKLFFGYATGTNLLFPFAVGGATALFSERSTAEKMFEV
                     IKRYRPTILTTVPTMINSMLNVSGASAEDVSSLRFCYSAGEALPKELYERWMNAFGID
                     ICDGIGSAEMFHIYITNRPGDIKPGSLGRIVEGYEARIVDADDNEVATGDMGTLKIKG
                     DSAALCYWNAHEKSKETFAGDWCTSGDQFHVDADGYYWYHGRTDDMLKVSGVFVAPAE
                     IENCLLQHEAVLECAVVGHESEGLVKPKAFVVVREGFTAADQLAEEIKQFVKEHIAIY
                     KYPRWVEFVESLPKNDRGKIDRKKLKHG"
     gene            complement(14523..14849)
                     /locus_tag="VFH96_04215"
     CDS             complement(14523..14849)
                     /locus_tag="VFH96_04215"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6973201.1"
                     /translation="MEKDRRSAPRARVKLSARWEGVLSRENATVTDLSRSGCFVLTGG
                     KVEVKELVWIEIQLTDRQAVNFWAEVVNQASEIGFALKFNSSSPEDEATLAAFLEKIF
                     QGQTKA"
     gene            complement(14849..15664)
                     /locus_tag="VFH96_04220"
     CDS             complement(14849..15664)
                     /locus_tag="VFH96_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET6973202.1"
                     /translation="MPNNYGDNPPLSYNKYLRVQDLINLQDCLSEPAHHDELLFITVH
                     QAYELWFKQILHELDAAAQFMEEDRVPAATRAIKRVVEIEKLLVNQIHILESMTPISF
                     LAFRDQLNPASGFQSMQFREIEFSSGLKDKNILREFSTDEFAYERLQARMSQPTLAEC
                     FYRVLQRRGLDANDYEQRTRAALEVLTHFEDRYEEFQLAESLLEHDEYFSLWRSHHIK
                     MVERMVGAKRGTGGSEGIGYLKTTLDKKFFPELWEARTHLDLTHGERGCPFAH"
     gene            complement(15665..16852)
                     /locus_tag="VFH96_04225"
     CDS             complement(15665..16852)
                     /locus_tag="VFH96_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6973203.1"
                     /translation="MSDDLLKWRSEFPILDKAVYLISHSLGAMPRATYDRLHDYAETW
                     ATRGVRAWAEGWWEMPVTVGDEVAQIIGADPGNVVMHQNVSICQSLILSCLNPTPQRN
                     KIVYSELNFPSVMYVYEAHARDGSIRIETVKSEDGMTVPLEKMLAAIDETTLLVPFSH
                     VLFKSAFLQDAKAIIDRAHEVGAMVVLDTYQSAGTVPFSVKELNADFATGGSVKWLCG
                     GPGAGYLYVRPDLQDKLEPKTTGWMAHESPFAFDTSLRYAPNITRFLHGSPAIPALYA
                     AQSGYQIINKIGVDKIREKSMRQTEYLINLAEEAGFTVTSPKDVKQRAGTITVAHEYA
                     AAITKELIHREFIVDFRPGAGIRISPHFYNTDNELEFVINEMKKIAESESYREHEAAG
                     AAF"
     gene            16928..18364
                     /locus_tag="VFH96_04230"
     CDS             16928..18364
                     /locus_tag="VFH96_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HET6973204.1"
                     /translation="MWKFANPIRHKILDTRWPTAEEAARSRLFSPIAIGKLEARSRTW
                     VPAMVPWRATEDGFVTQQNLDWYRRFAEGRPGVLVVEATGVRDIPSGPLLRIGDDRFI
                     PGLRKLVEVVREASGGETRLFIQIIDFLAVKRRPEKEKFFQRFLTVEDHHRQALMELT
                     SQSAWLRAPDDHVREFLLNADEEIIDLVLRPRELEALRFGYRERVSDTHLKHIRDLPQ
                     ELPGIFAAAAERAREAGFDGVELHYAHAYTMAGFLSAQNDRADGYGGSQENRVRLPLE
                     VYREVRNRVGSDYVVGVRFLADEVIAGGSRVEDAIFYAKEFAAAGFDFLSLSKGGKFE
                     DAQQPKVGQAVYPYTGQSGYECMPTVLSDEVGPFGRSVPLVAAIKKAVSESGFATPIV
                     ATGGISTFEQAEEVLRLGQADIVGMARQALADPDWFMKVKLGRGDEVRRCTYTNYCEA
                     LDQAHKPVTCKLWDKGRRVLEPPPWSSV"
     gene            complement(18353..19489)
                     /locus_tag="VFH96_04235"
     CDS             complement(18353..19489)
                     /locus_tag="VFH96_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6973205.1"
                     /translation="MKINVIGGGPAGLYFAILMKKADAAHQIRIYERNGPDDTFGWGV
                     VFSGKTLANLRAADDVSHGEITKQFEAWDNVDVVHRDAKISIHGNSFSGIARLQLLKI
                     LQRRAEQLGVEIYFHTEIHDVEALRNDCDLLLAADGVNSTVRLRYQEQFQPELDSRAN
                     RYIWYGTHQLFHGLTLTFRENSAGVFAAHSYKFSPTTSTFIVECDPQTWANAGFEQMS
                     SEDTLDYVGDVFAQDLNGHKLLSNNSKWINFLLVKNRKWFFENVVLIGDALHTAHFSI
                     GSGTKLAIEDAIALAESFQQTTSVSDALTHFEKTRRPIIEDYQAAAFESMRWFENAAR
                     YMHLSPIELAYVLMTRSGRVSYEDLKRRDPAFIREYQLATDLHG"
     gene            complement(19570..20718)
                     /locus_tag="VFH96_04240"
     CDS             complement(19570..20718)
                     /locus_tag="VFH96_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6973206.1"
                     /translation="MDRLISQLPFFTPEHRDLAQSVAQFVAAEIEPRAAVEENVEDAA
                     RDFVKLLAGAGLLKYAVAESKIDVRSLCLIREELAYSSALADLAFVMQGLGTYAIARA
                     APEHVRDFWLSRAANGKAIAAFALTEPNAGSDVAAIETTARHDGETYVIDGRKRFISN
                     AGIADFYIVFAKTGTREDGRPVLSAFVVGSKMPGFRVVERTSLIAPHPIGEIVFSECR
                     VPAEDMVGEEGDGFKLAMETMDTFRASVGAAACGMARRALDQAVRYASGRKQFGRLLS
                     EHQLVQAKLADMITELDAARLLVYRAAYARDTETGRVTREASEAKLFATEAAGRIIDS
                     AVQIHGGAGLVSGTVVERMYRDVRALRIYEGTSEIQKLVIAGQLLKEN"
     gene            complement(20718..21512)
                     /locus_tag="VFH96_04245"
     CDS             complement(20718..21512)
                     /locus_tag="VFH96_04245"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET6973207.1"
                     /translation="MKQPTTFLYEQDDSGIATITLNRPDRLNALTFEVYRELTDTFAA
                     LRDERAVRVVVITGAGRAFCSGGDVHDIIGELFSRDMEGLLEFTRMTCELIQNMRALP
                     KPVIASLNGTTAGAGACIALASDIRIASEEAKIAFLFVKVGLAGTDMAASYLLPRVVG
                     LAKATEMLFTGDFVSATEAERIGLYNRVVPPAELAATTRAFAEKLARGPAFALGKTKE
                     MLNRELEMNFQTALESEAQAQAICMQHPDYREAYEAFVAKREARFQ"
     gene            complement(21509..21913)
                     /locus_tag="VFH96_04250"
     CDS             complement(21509..21913)
                     /locus_tag="VFH96_04250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET6973208.1"
                     /translation="MSFKLINPEALGAPSGYSNGLLVDGGGKLLFIAGQIAWDNNQKI
                     VSDDFVEQFDQALANVMTVLRAAGGEADNIARLVIYVTNKIEYRERTREVGDSYRKHM
                     GKHFPAMVLVQVAGLLDDRAKVEIEGMAVIPA"
     gene            complement(21970..22455)
                     /locus_tag="VFH96_04255"
     CDS             complement(21970..22455)
                     /locus_tag="VFH96_04255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6973209.1"
                     /translation="MTLLATSVALAQNPARADFSLRSIDGQTVTSDSLRGEIVVLAFG
                     ASWLPLSKTQLQGVRKLADEYSNRGVVVYWVSTESDDSKSKNFASDEQLRSFANKYGL
                     KVTVLRDPDGTVSKKFGVDQLPSIVILDKQGNMVDGPIGGLDPNGNLAAQLASRLDKL
                     L"
     gene            complement(22560..23168)
                     /locus_tag="VFH96_04260"
     CDS             complement(22560..23168)
                     /locus_tag="VFH96_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6973210.1"
                     /translation="MKSCSAILLAAGRSSRMGAFKPLLPFGNKTVIECCIDYLRAGGV
                     EEIVVVLGHRADDIRQQVSGVKFALNPDPDSPMGASIAAGVRELPETAQATLIVLVDH
                     PAVAPTVVSTLLESWKSGARLVIPTWQGRGGHPVLVDLSFKAELLNLPEGGGMRALFD
                     AYRNEITRVPVASPFIARDMDTWDDYTSLHLEVTGRSAPRPT"
     gene            complement(23201..24181)
                     /locus_tag="VFH96_04265"
     CDS             complement(23201..24181)
                     /locus_tag="VFH96_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET6973211.1"
                     /translation="MMRLPKFEYHVPQTIAEAVRIRGEAGAAGQFVAGGTDLYPNMKR
                     RQQTPRTVISVMRLPELNQITGDGNSGLHIGASVTLTEICEHPIVNRDYPVVAKAART
                     ISTPLLRNMGTVGGNLLLDTRCNYYDQNYEWRKGINFCLKKDGDICWVAPGSSKCWAV
                     QSSDLVPVMVAIGAKLRFASTLGERVIDAAGLYNDDGIEYLHKRPDELLVDIQLPPTN
                     GWRASYQKLRRRGAFDFPVLGVASWIKTDNTTVTDARIILGGIAPSPVEIKEAATALI
                     GKPLADDQIHAAAEAAYIKARPLDNTDFVYQWRKQMARQYTLRALRDLQQ"
     gene            complement(24190..24411)
                     /locus_tag="VFH96_04270"
     CDS             complement(24190..24411)
                     /locus_tag="VFH96_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973212.1"
                     /translation="MNEEKPSHRPLVFSVVEDDGEDVTIEISEEDYLADLAAGIDEDA
                     TLKPGRYKGKRGGFLARHREMKIKEKKRA"
     gene            complement(24714..27134)
                     /locus_tag="VFH96_04275"
     CDS             complement(24714..27134)
                     /locus_tag="VFH96_04275"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET6973213.1"
                     /translation="MNIIGKPFRKVDARAKCVGQTKFADDIVLPRMLFCKLLRSHLPH
                     ALIKSIDVSKALALPGVFAVVTGKDFPIPYGILPVSQDEHALCVDKVRFIGDPVAAVA
                     AVDEDVAFDAMNLIEVEYEPLNTISTIEEAVLIDEPRIHDYGDGGNVHKKVSLEFGNL
                     EEGFAEADLVREDTFFYEGNTHLPMEQHAAVAHFDSDGKMTLWSSTQTPHYVHRALAK
                     VLGLPASHIRVIATPNGGGFGGKSDPFNHEVVVCKLSMITGRPVKVTLTREEVFYCHR
                     GRHPVLMHVKTGVKNDGAITAMHFQSYLDGGAYGSYGVASTFYTGALQTVTYEVPRYK
                     FQGIRTFTNKPPCGPKRGHGTPQPRYALEVQLDKIAEQLQLDPVEIRRKHLVAPNSVT
                     ANYLRIGSMGLGACIDKVVEGSRWKERFSWDQNNRKLPFGKGIGIACSSYICGAGLPI
                     YWNNMPQSGVQLRLDRQGGVCVMCGSTDIGQGSDSILAYIVAEVLGIDPFDIRVVTAD
                     TDLTPVDLGSYSSRVTLMTGNAAIQAAERARELLVMAVAEKLGIPIENLSLAERRVFD
                     VENPEVGVSFAEAVVLAESKFGTIGTVGSYTPPRSPGKFKGAGVGPSPAYSYSAAVAE
                     VDVDPDTGIVVVERVWIAHDIGRSINPVLVVGQVEGSVYMGLGEILMEEMVYRENRNV
                     VHKFPSLLEYKSPTTMEMCDVKTYLVEDPDPNGPYGAKEVGQGPLLPVPPAVANAVYN
                     AVGVRIDEVPITPEKVMRALKDKSRGREGRYGPDNVPPVAWPEPLKVLTPAEGGDGSE
                     MPRVAVHS"
     gene            complement(27174..27698)
                     /locus_tag="VFH96_04280"
     CDS             complement(27174..27698)
                     /locus_tag="VFH96_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6973214.1"
                     /translation="MPLQSKKHIHFTLNGEHTEVAFAPHKTLLEVLREDLNLTGTKHG
                     CELGECGTCAVLVDGRSILSCLMLGLEAEGREVKTIEGMADGAELHPLQQTFADLGAA
                     QCGYCSPGFLLVAEELLQKSPNPSRAEIQEALSGNLCRCTGYIKIYEAVELAAARIRG
                     DEMELPKESVYGLD"
     gene            complement(27682..28563)
                     /gene="panC"
                     /locus_tag="VFH96_04285"
     CDS             complement(27682..28563)
                     /gene="panC"
                     /locus_tag="VFH96_04285"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET6973215.1"
                     /translation="MEIINRRQRMSSVARKIRREQDKTVGLVPTMGALHEGHLSLVRE
                     ARRMCDVVVVSVFVNPTQFGPGEDFEHYPRDLTKDTALLTDYNVDYIFAPTVEEIYPK
                     GFSTYVKVEGLSEQLEGHSRPGHFRGVATVVTVLLNTVRPDFAFFGQKDAQQALVIKR
                     LVKDLAFDTEIVILPIVREDSGLAISSRNLYLTADEQNSATILHKALKQAKTAFKEGE
                     RNAGRLADIIRSTVETEPRARLDYVTIADAETLEKLDRLDDRPTLIALAAYVGKTRLI
                     DNVILNKVKKKDVAAPK"
     gene            complement(28579..29472)
                     /gene="panB"
                     /locus_tag="VFH96_04290"
     CDS             complement(28579..29472)
                     /gene="panB"
                     /locus_tag="VFH96_04290"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET6973216.1"
                     /translation="MPYLKPERPEKVSAPSLRSSKQRGERLVCLTAYDYPTARIVDEA
                     GIDIILVGDSLGNVVLGYGNTVPVTLEEILIHLKAVRRAVNRALLVADMPYGTFHTGD
                     DDAVRNALRLVKEGGAEAIKLEGGHKRVHLVKRLVDEEISVMGHIGLTPQSINQLGAY
                     RVQGKTASAAQQLIDDAKAMEDAGAFAVVLEVVPREIAQMITESISIPTIGIGAGVHC
                     DIQVLVIHDMLGLSFGKQARFVRPYANLREVMTDAITRYAEDVRNGTYPSEAESYALP
                     AQTAAELDIEVPSRDSKAEKS"
     gene            complement(29671..30156)
                     /gene="folK"
                     /locus_tag="VFH96_04295"
     CDS             complement(29671..30156)
                     /gene="folK"
                     /locus_tag="VFH96_04295"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET6973217.1"
                     /translation="MIQNAYVGLGSNLGDRAGYLLLAVRGMLDAGLDVIRLSSIYETE
                     AVEYEEQPAFLNMVAELRGSTLPSPEQTLARLLRIEYALGRTRDVRMGPRTIDLDLLI
                     FKDARSDTEFLKLPHPRIASRRFVLVPLAELVPNLVLGVPIRELLAETKDSSSVVRWK
                     A"
     gene            30163..31833
                     /locus_tag="VFH96_04300"
     CDS             30163..31833
                     /locus_tag="VFH96_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973218.1"
                     /translation="MSQLLTLIWLKWRLLRNSLRSSKAVVNRAASILGMLIALFLALI
                     VALILGFAAYGLSQPEALGNAFRRTATRDVSASASAEFIFFSMFGLIYLMWATVPLSI
                     GGGKQFDAGKMLMYPITLRKLFAVDFLSELTTLHSVFAIPAVIAICAGVALGTGRTTA
                     AAVAAVPAILFGVALSKWLSTTIGSLLRRKRARGETLVALIGAVAGLGAAVLGQIAPI
                     LFTHAESFRSLRWTPPGAAAYLLVGPPNDVAGYVVAFITLSAYGVALVIATYWIARRA
                     ALGIEGRRRQKTAVVAETATGYSGWQLPLVSPELSAIVEKEVRYAMRNAQVRMMALMP
                     LVLLVVRVMNTKKWWGTASPSGSFLTYGSGLLATGGVLYVFLILAGLSCNHFAFEEGG
                     MRTLILSPIDRRKILLGKNIAITLLALIFATILLTLNALVFRDFEASTLLFVGLSFVN
                     FAALSAMMGNWLSIRFPKRMRFGKRLNVSGVAGLLLIPMVIVLGAPPVLATLVGLFMS
                     SFLYEYLALLLFALLSIGFYLPMLNLQGRSLAKREIDILEAVREPADE"
     gene            31978..>33104
                     /locus_tag="VFH96_04305"
     CDS             31978..>33104
                     /locus_tag="VFH96_04305"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HET6973219.1"
                     /translation="MARRRFRSNQRHEPTTERSFQEYILSTPAPQTSRTELLRLIRGG
                     EDTFLELKVKLSNTEKIAQEIVALANTGGGVLVFGVNDQLRVEGVDDPERVQEDLVRI
                     CREEVQPPLVPFIDRIALDNGRRIVALDIESKRRPYRTRDGRFYLRIGAEKREASREE
                     ISSLLEESRPLRYEDVPALGSTMDDIDEAHLWSYLREFEGGAFEEATVVGYPTADVLE
                     RDLLLATNDGNDIVPTVAGLLLFGRDERVTELLPRASVTATRFSGDSLQSPVVERVKF
                     SGNLVSIYEGLMRFVRRYCDLAEVRPKSSALTDESPVPARASYHGGVVAEAITNLLAH
                     RDLALRDLPSRLNVFDHSLEFVNSRRSVGFSPVAQKAIRYG"
BASE COUNT         7291 a         9143 c         9160 g         7510 t
ORIGIN      
        1 gtggatttcc cttatcctgt tattccgatc gtcaggcatc atcacgaaca gtgggacggc
       61 cgtggttatc ctgaccgtct gcgcggtgaa cagattccca tcaccgcgcg cattatctcc
      121 gtggtcgatt gcttcgactc gattcgcgaa gatcgaccgt ttcgccgtgg catgacgctc
      181 gacgaagcta ctgcgttgct gttgcgcggt tcgggaattc atttcgatcc ggtcgtggtc
      241 gagcagttca tcaaacactt gccgcgtttt gacgccgaga tcgagcgcct cggcctgcaa
      301 catcaatgtg caaactattc aaccgagccg ccgattcaat tgagcgaagt tgatatgagt
      361 cagacgcgtg agcgcggctc ttacatcgct tacgatcaga tcaaaaaagc gcatcgcgaa
      421 gtttatgcgt tgtacgagat tgcccgcacg tttggcacgt cgctgaacgt cgagcacacg
      481 ctcgagattc ttgttgacaa ggtgggccac gtagtcccct tcgacacgtg catcgtttac
      541 ttctacgacg atcagaaagg ttacgctact gcgcgacacg tcgccggtaa gcatgtacag
      601 aagctggccg gacgttgcat tgcactcggc gaaggcgtca cgggctttgc actcgcgaat
      661 cgaagcgctg ttaatcagct acatcccagt cttgatttca ctgacataaa tcccgaagct
      721 ggcgttaaat atcgctcgat ggcgtcgttg ccattgttca aggacgacgt gttgctcggc
      781 gcgctctcgg tctactcagc ggagctcgag caatacactg acgatcacat gcgactgctg
      841 gagactgtga cgcggcttgc ttcggacgcg ttgggaaatg cgatgcagca tgctgaagcg
      901 gagtcgaatg cgttgaccga tccgctcacg ggattgccga acgcgcgtta tttgtctttg
      961 cggtttgaag aagaagccgc acgtgcgcgt cgcaccgatc gtacgttcca ggtcgtgatg
     1021 ctcgatctcg atgagttcaa gaacgtcaac gatacttacg ggcacaaagc aggcgacaag
     1081 atgttgcgcg aggtggcgca cctgatcgcg ggccagttgc gtgagtatga cttcctcgcg
     1141 cgttcaggtg gcgatgagtt tgtcgcgctc gtgcaggagg ttgtcgggtc gcaggttgaa
     1201 gatttgtgca tgcgcatcga gagtgcggtg tcgaagttct ctttgccgct cggaagaaac
     1261 cgttctgcgc gtgtcggaat tagtatcggc acggcgacat tcggtgtcga tggcgacacc
     1321 ctggaccagc tcgtcgtcgc cgctgataac gagatgtacc gcatgaagtc gacccacaga
     1381 tctacgcgga ttgttgaaga gccgattgat accgatctgc agaattcgtc agcacctgcc
     1441 tgttgacact ggaccagtac aggagtaaag ttttcgcccc ccaccgttcc tgggctgatg
     1501 cacggcaatt tctacctcgt taacgtttcg tagatgagtg agtacttgta cgcatttaag
     1561 ttcctttaac cgatatcggt ctatctgaat caaatcaagc ggtcatagtt tccacgtcct
     1621 tactggagaa ctccctatgt cccgtcgcgt tgttccacgt ctcacacgcc gaattgccat
     1681 tgccttgaca ctctgcatcg ctctgctgcc agcgcaccac atctggttgc tggccaccga
     1741 agcagctcag ggccatcccg aagaatccga gcaaaaaaca aagccgaagc caggcaagcc
     1801 agagggtatg ttgcctaatc tggaggacgt acggaaagag aagcagcttg aacgcccgtc
     1861 gccgccacct attccctcga ctattcgctc gaaaaggaat acaggcaagc cctgggatgg
     1921 acgacgtgtc ggtgatccct ggcctgaagg aacttcgaac caggctgtta acaatgccga
     1981 ttcaatctca ccaatgcgca gccagcggac acggcgagct catgcgcgga tgcgagtcaa
     2041 cccttcgcca cttgcatggg acgatcagtt tgtacagaac ttttttagct ggaccttggc
     2101 gcgcagttct tttagcgatg aaaacaccta ctggtatgat cacttgcgcg tggcctacgg
     2161 gcagggtcag gaggcgttga agcttgccgc tatcgagttt ggtagaacgc tgtttgaatc
     2221 ggctgaatac gacgctcgca accgctccaa tcactggtat gtctacgatc tctacaaggc
     2281 ttatttaatg cgtgatccgg accccggtgg gtgggcgacc tgggaagccg tagtggccac
     2341 gaacggacgc gagtacgggc ggcgaggttt tgaagagtcg ggtgagtttg ccaccttgat
     2401 tgcgaatata ctgcccaacg gctcggctac aagtaacgcc gcgtcattaa tctccgcacg
     2461 tgtggacgcg cgcaaccaac ccggcaatgg aatgctgacg cgtgatgcga cctggagtat
     2521 accgctgtta agcttgccag gccgcgccgg actcgatctc ggtctggggc tttcgtattc
     2581 ctcaatgagc tggacccgct ctggaccata catctatttc gatgaagata acggctttcc
     2641 cagtcctggc ttccggctcg gattccccat cgtgcagcaa aaaaccttcg acgcacaaac
     2701 cgctagaaag gcttacctgc tgattacggc tgccggtaaa cgagtggagc tgcgacaggt
     2761 gggttcatcc aatatctatg atgcggctga ctcttcctac ctgcggttga ctgataacgg
     2821 cagcctgagg gttcagtcca ctgatggtac gaagctgact ctcacatatt tcaatggcga
     2881 atttcattgt actgaggtca aggacagcaa cggcaactac attaccatca actacaattc
     2941 actggggcag ctcacgaaca ttactgacac acttgcacgc gtcataactt tcaactacga
     3001 cggcaatgcg aacctgctgt cgatcacgca aagctgggcc gggcagccgt cccaccagtg
     3061 ggtcagtttc ggctggagta cgcgcgcaat gcagtcaagt ttttctgacg cttcgttgcg
     3121 cggcgtaatt ggcacggcaa atgggaagag tgtgccagta atcacgcagg ttgggctgaa
     3181 cgacacgtca catttcactt ttgattacaa caactcgcta caggtgtcgt ggctcaagaa
     3241 ctacttcgac accctcgaac gcaacgccac cactttcact tacgaaacat cggccggcga
     3301 tgcgcctcga ctgctcgaca gtcgtgtgtc ggccctcaat tggacaggca taaacaatgt
     3361 cccagcgcag gtgatcacgc agtacggcgt cgctccggac ggtgcttgca caatgacggc
     3421 gccggacgga actatttaca aggagtatta cggcactggc tggcaaaagg gtctgaccac
     3481 gcttagcgaa atctggtcgg gaggcgtgcg caagaagtgg atcacgaccg catggacgca
     3541 ggacgacacc aatctgtctt atccaaagaa tcctcgtcct tatgacacca gtatttatga
     3601 cgaagcaggt aatcgccggc gggttgaaac catttacacg tcctacaatc tgcccgatgc
     3661 tgtcgccctg cccaccgaag tgaaagaata taaggccaac ggcacggatg tctggagacg
     3721 tactaccact atgtactatg acggtgggca gccttatatc gatcgtcgcg tgctgggtct
     3781 tgtgcgagag gtgatggtgt acgatgagaa caatcagccg ctgtcaagag tctggtatga
     3841 ctacgactgg aacactcccg acgcctgggt tgcaactccg caacccgccc cgcaacacga
     3901 tcccagcggc gatgccaggg gacgcggcaa cctggcttgg atgggacgtt gggatgtcaa
     3961 cgactttgac aacttcgaca agaactctaa aagctatgtc aaatacaacc gcaccggctc
     4021 tgtgatcaaa actgaagatc actttgggca cggtaagacc atcagttaca ccgactcatt
     4081 ctctgatgga gtgaccactc gctacaccta tgcttacccc actacgatta ctgatagtga
     4141 cgggggcagt tcttacgtgc agtacaacgt cgataccggc gccacgacac gcacggaatc
     4201 tccagcaccg gccggccaga cacagggtcc cattcagacg atgacttata acaacctggg
     4261 ccaactggag cgactgacta cggccaacaa cagtgcttac acgcgttact actatggtcc
     4321 ctatttcatc cagagttatg ccagcgtgaa taacatcgcc gatgacgctt acaccaaccg
     4381 cgtgtttgat ggcctcggac gtgttagggc agtagccagc aatcatcccg acagcgcggg
     4441 tggctacaaa gcacaactga cggtatacga ctcgatggga cgtgcagccc aaacatccaa
     4501 tccggctgag atcaccgagc tctgggcgcc ttacggtgat gacaatgcgg gctggctcta
     4561 cacgcaacag tcttacgatt ggcaaggccg gccgcgtatc acgacaaata cggacttatc
     4621 aacgaacgag atcagctacg aagggtgtag ctgcgcgggt agtgacgtca taactacaac
     4681 ggacgctgtc gctcgtaaga ctaagtcata tcgtgacgtg ttggggcgcg agtggaagtc
     4741 tgagattctg aacactaacg gtactgttta tgctaccact atcaacgctt acaacgggcg
     4801 cgaccaaata ttgagcagca agcagtatgt aggactcgcg actagtgatg gctcgtgtcc
     4861 tgttaacact tgtcaggaga ctacctatgg gtacgatgga catgggcgct tgtcatcgcg
     4921 acacctcccg gagcaggcca gtggcacgag tactgtctat acctacaggc ctgacgatac
     4981 cttatggacc gaaacggata gtcgaggcgc gacaaccact tacacctaca atggtcgaga
     5041 cctgccgatc accatcacct acactgcgag tggcatctca gtcccctcac ccacgactat
     5101 agaatacgat gctgcaggta atcgaagctc gatggcggat tcgatgggta ccgtgtcgta
     5161 cgactatgac cagttgtcac gtctcaagca cgaaaagcgt tatttctcta atttaccgaa
     5221 cgctccggtc cccgagcaca aatacacgat cacttacgac tataatttgg cgggcaattt
     5281 gacgagcatc acagatccgt ttggcgacag cttctcctat acgcgaaatg ctgtgggaca
     5341 attgaaaact gtctcaggct caccttacgc cggctggacc agctacgtca ccaatgtcac
     5401 ttacagggcc tggggcgcgc caaagggtgt tagttacgta ggagggagct caacaatttg
     5461 gtttaattcg cgaatgcaac catcagcgtt tacctcgact gctgccggaa tgaatgagag
     5521 ttattcctac tatgcggatg gcagacttca caccctgact gacttaaacg ataccgctgg
     5581 tagcaatccg ccggtaacct tgcgcttttt gagtcgcagt tataactacg atcacgtggg
     5641 ccgagtaaca aacggcttcg gtaccggaag tggcgctccg ggtgtgccgt ttacacaaac
     5701 gtactcgtat gacgcgttcg gaaatatgac cggacgaaca ggctcatact acaactacca
     5761 gtccgactcg agtctgacaa aaaccgacac ctcaacatac gttaacaatc gccgcacaag
     5821 ctggggctac gatctcgagg gacaagtaac atcaactccg cttaccagca ccgatagtcc
     5881 ccgtacgatg gcttatgacg cggtgggtcg tatggtgagc agcgtcgaaa caggagcatt
     5941 gaacacgacg acttacacct gtgcatacga tggtgatgac caaatggttt ttgaatcgag
     6001 cactacgtcg ccgggcactt cagagtcatc ttacctcgta cgttcgagtg tgctgggcga
     6061 tgtactaaca cgactggacc aatcgggaaa caagaagatc acttacgttc cggccgaggg
     6121 actgctattc gccacccaaa gggcgtcggg cggacctggt ccatttgtcc tgcccacata
     6181 ccgcaatcca ctaggtatca gtgaaacgac caaggccgtt tacgatcctc tcggaaacta
     6241 catacctttc gaggcccacg acgatccacg accacctgca ggtagttata gttccgcttc
     6301 gatgtccgga ctttcgtcca gccaagcgaa tgctgagagc tacggagtgg gctgcctgat
     6361 ggataatgtg ccgacgacct gcaagaaggt catggaccac cttgaaaagg agcgagcaca
     6421 aaaactgcaa gtgatcggtc cagcttcaac ttcattcatc actagactgt tgatgagtgt
     6481 tacagcgggc accgcatggg ttccagttgg gccgaaaaag ctaattcagg tcttcgaatt
     6541 gaagaatggt aaggctttga agatttatag ccaagcaggg aagctttgga cttactatat
     6601 agtagcgccc ggctcgccgg atcgctttaa gccaaataca ataagcaagc agccggatac
     6661 cgtgactgac aatccgccct caattgacaa cagcaaggcc aaagaccaag cttgtcagat
     6721 tgaagtttca ttcaccggct caggttcacg aggattgcag aatggaccaa gcacgatgaa
     6781 cctcaacaac cggggtcaag tatttgggat tggatttact gtcaatattt caggcctatc
     6841 gggaccgata aatattgtga gcgaagcaaa ggacaggcgt ccgaacgccg cttggaatgt
     6901 tgaacagtat gtggcggact tcaataagag taatggagtg gttgtaagac aggatggagg
     6961 agcgcgtttg gaggccttgg ggagggctgc tcctgtggtt gacggtggta cagtcgagtg
     7021 gtgggatcat cctggcacca ctgtttcggg gaacagccaa tacttcacca aacgaaactt
     7081 ttacattaag gcttacaagg gcaagcaaca ctgtgaggtt tcgtttcacc tcacttttag
     7141 attgtttttt gggaagccac aggatacagg atggggtgac ggcccctatc cgtgatgagg
     7201 tgcaattatg gggacattca aagtaatagg tcagttcggc tttctgccaa tctttgtctt
     7261 gctggtggta ttcactacac gagttgaccc acaacactcg gcaaacgcat cgcgcagagt
     7321 tcgccaggat agtgagtgtc tgtcgcccgg tgttctcaca aatgcactca agcggttatc
     7381 cgaaagctac gatgaagctc ggggcgcaca cgacacactt atcaaagccg ccggcaaatc
     7441 cgcacgatgt aaacaacaag ttgttttgtc gctaatgcaa gccatggata agcctgggtt
     7501 ggacatccgt cgcaatcagg gcgattacta cttgtggcgt gaaggcgcaa ctttgctcgg
     7561 cgaactgaag gcgacagagg cccttgatct gctaatttcg cacatgggaa tgagcgatgg
     7621 tgagtggagc gtgtcaatgg ttcatcaacc tgcgttaagt ggcatcattg agatgggacc
     7681 aatcgcgatt ccaaagcttg gcgtggtgtt gggcagtcag gatacagcca cgagacacga
     7741 cgcggtttat tgtattgcgc agattggagg gccagccgct cttcgagtgc tgaaacaagc
     7801 gcttccttca gagtctgaca agtgtatcca gcgttttatt caagcttctt taaagagctt
     7861 ggataacgaa aagaacaaat tgaaggacaa cggagaatgg cttccagcct ttttgtgcaa
     7921 ttaaagtctc aggagtttgc ttaagccaac cgcatctaac aaattaaatt taaataattt
     7981 gagctggtaa acggcagaac tctgcacagc agccgatcga tcctccgtcg agaacgtact
     8041 tctccagtgc gcggtggagt gaagattggt tcgaggtgta atcgatatgg gtgagcttgc
     8101 actgccaaaa ttacaaactg cgttaagaga taatcctgat cgctttacga ggcgattaac
     8161 agtcttttgc attgccctta tcggaggccg aaactttgct cggcgaactg aaggcgacag
     8221 aggctcttgg tctgctaatt cgcacatggg aatgaccgat ccctttttaa gagaaatctt
     8281 gacatattcc tcactcatcg ctttcaatcg ctatgaggca tgtcttcatg acgcgtctct
     8341 gctgaaagca tggtagctcc cgccgaaaat cacagcgcca cactcttaat tgttgacgac
     8401 gacgcggccg cacgcgatag gtttcgggaa atcttcgagg ctgcgcagta ccgtgtcgtt
     8461 actgccaacg aagctgcctc ggcgctacgc gtgctcaagg gcgttcgttg cgacctcgtc
     8521 gcgctcgatc tcgagatgcc gggcgtagat ggtctcgccc tctgcaaact gctgcgcgcg
     8581 caaccggcca cgagcaagct gccgatcatc gcgctctcgg aaagcgacga cgaggcacgt
     8641 aaggtccagg cattcaccgc gggcgctgac gactacatca cgaaaccttc aacgcccggg
     8701 gagttgttgt cgcgcgttag cctgcacgtg cgcacgtcgc agcgcgagtg ggccctgctc
     8761 ggcagcaatc atgagctcag gtttttgtca gacatcggtc gcggactgtt gcgcgcgctc
     8821 gagcccgagc agctcgtccg tcgtgtcgcg ggggcgactt atgaagcgat cgaaagctcg
     8881 ctttgcgcag cttatgtcac ggttggtaag gacaaagatg cgatgtgcgt cttcgatcga
     8941 gaaggcgctg ctgacgatcc gacactgtta aataccgaag cgttacaaga gtggctccgg
     9001 tcggcggagt ctgcaaactc attactgatc actgaaaaga gttcattcct gctgcgcgac
     9061 gatgttcacg ctgcggagta cgtctcgcca tttcgttttg ccggtcgcgc gaagggcgcg
     9121 ctgattgtcg ccttcgatcg acgtgaagaa tgcggtgaga ccgaacgacg tttgatcgaa
     9181 gcggcggcgc agcagacatc tctcgcagcg cacatctgct cgctttacca gtccgcgcgt
     9241 gatacgtcgg taagcctggc gcgagaagtc gaacggcgca ccgcggagag tgaagcgcag
     9301 gcgagattca tcgaagcaat cgtcgacagt ctgccactct cgctttatgc aatcgatcgc
     9361 gagtatcgcg tcgttgcgtg gaaccgcaat cgcgagctcg gcgagctggg tattccacgc
     9421 ggttcagtgc tcgggaagaa cgtgttcgac gtgctcacac gccaaccacg cgacatgctg
     9481 gcggaagagt ttgcgcgcgt gttcgagagt ggtgagatcg agcgcatcga acaggaaact
     9541 ccttcgccga aaggcgacat ccgccattgg gtgatcagca agattcctat gtgggctgac
     9601 aacaccggcg aagtcagcca cgtgatcact gtcggcgagg aagtcacgga tcgagtcgaa
     9661 gcgaatcgcg cgattgcacg cgccgagaaa ctcgcggctg tcggtcgcct cgcggcgggc
     9721 gtggtccatg aaatcaataa tccgctggca acgatctccg cctgcgccga gtcgctcgag
     9781 gcgcgtgtta acgaaggcgc attcgaaggc tctgctgcag cgctccagga tctgcgcgaa
     9841 tatctcgggc tgatccgcag cgaagcgttc cgctgtaaga gcatcacgat gggcctgctc
     9901 gatttcagcc gcacgcgtac ttcggaccac gtgacgatca atctcgcgga cgttattcaa
     9961 tctgccgtgc gcctgctttc acaccagaaa cgcagcccct ttgtcgactt caaaaccgag
    10021 attgctaaag atttgcagtt tgtttcgggc gacccgggcc agttacagca agccgtaatc
    10081 gcgctcgcga cgaatgcgct cgatgcgatg ggcgacagcg gcacgttgac gatcgtcagt
    10141 cgcaacgaag gcgacaaagt ggtggttgat gtgaacgaca cgggcgtcgg cattccgccg
    10201 gagaacctgc cgaagatctt cgagccgttc tttacgacga aggaaattgg tcgtggaacc
    10261 ggtctcgggc tggccgtatg ttatggtatc ttaacggagc atggcggcac tcttgacgtg
    10321 cagtcaaccg tgggatcggg tacgactttc acgattactc tttccgcagt caagataagc
    10381 gaggaacaga cagtttgagt gagcagcaac agcaggcacg aattctgatc gcagaagacg
    10441 aggccaacct tcgactcgtt ttgcagaagg aactggagag gctgggctat cgagtgcagg
    10501 ccgcgcctga cggtgaggcg gctttgcgca aactcgaaga gagcaatgtg gacgtgctgc
    10561 tgtgtgatat caacatgccg cgcatggatg gcatggagct cctgcggcgc gtttatcaac
    10621 ggcccaaccc gcccgaagtg atcatgttga cgggccaggc tacggtcgag actgccgtcg
    10681 aggcgatgaa gctcggcgct tacgactatc tcacgaagcc ttacagcatc actgagctcg
    10741 acgtgcgcgt gaagcaggcc gccgataagc gacggctgcg cgtcgataat gtgcgactac
    10801 gcgaacaact ggcgcgacag tctgcgttgc ctgagatcgt ttcggtgtcg gaagtgatga
    10861 aggaagcgat acggttgatc gagcgtgtgg cgccgtcgga agcatcggtg ttgattacgg
    10921 gtgagtcggg gacgggaaaa gagctggtgg cccacgcgat tcaccgtctc tcgaatcgtg
    10981 ctgaggcatc gtttgtcgat ttgaactgtg ctgcgtttca ggagtcgttg ctggagtcgg
    11041 aattgtttgg ttacgaggcg ggcgcgttct ccggtgcgaa aggtcgaaag ctcggactga
    11101 tcgagcttgc tgatgggggc acactctttc tcgatgaagt gacagagctg ccggcgcagt
    11161 tacaggcgaa actcttgcga gcgatcgaga cgcgtacgtt tttccgtgtt ggtggtgtgc
    11221 gcaaggtcga ggtcaacgtt cggattgtcg cagcgacgaa tcgaaatctc gacactgtcg
    11281 tcaacgacgg cgtgttcaga tccgatctgc tttatcgcat caatggtttt cagatcagtt
    11341 tgccgccgtt gcgtgagcgc cctgaagaca tcgacccgct ggcgcgttac ctgctcaaac
    11401 aaattgcggg aactcagccg ccggagctta ctgaagacgc gttcacggcg ttgcgcatgt
    11461 atcactggcc cgggaatgtt cggcagttga agaactgtct ggagcgtgcg gtgatcttgt
    11521 ccaacaacgg ccggatcacg ctttcggagc tcccgcctga agtgacgcga ccggctgtgc
    11581 ctgcgccggc gttgacgagt gtcgcgcctg cggtggtggg cggtgaggta gtgactattc
    11641 cggctgcggc aattccatca tctactggat cgttaagaga agttgagcgg cagcagattc
    11701 tcgccgcgct cgagcagact ggctggcacc gcggcaagac tgccgagatt cttggcattt
    11761 ctccatccac gctctatcgt cgcttgcgcg attacgatct cgaaagaaga cgttagccac
    11821 agattacacg gataaaaatg cggattagat ggggcctgat cggatgtggc gacatctcgc
    11881 gcaggcgtgt tgcgcccgcg ttgcgtgatt cgccgctttg cgagctggtt gcggttagca
    11941 gagcgaaggc cgagcttgcg gaatcgttcg cgaaggaatt cggcgcgcgg cgatggtgtg
    12001 cagactggca cgatttcctg atggactatg agatcgatgc catctacgtt gctactcccg
    12061 ttcatttaca cgccgaacag gtaatcgctg cggctgagcg aggcaaacac gtgctgtgtg
    12121 aaaagccgat ggcgctcaac gtggctgagt gtgatcggat gatcgatgcg tgccggtcca
    12181 atgacgtcaa gctcggagtt gcttactatc gtcactttta tccggttgtg cggcgtatga
    12241 aggagttgat tgattcgggc gagctgggtg tcccggttgt cgcgcaggtg aacgcattcg
    12301 agtggttcga tccggaaccg ggttcagcac gttcgtggtt actcagaaaa gaactttccg
    12361 gcggtgggcc gatgtttgat ttcggctgtc accgcatcga agtgctgctc gatctgcttg
    12421 gcgatatcag cgacgttaaa gcaacgctcg ctaatgcctt cttcaatcgt gaagtagagg
    12481 acacagccac cgcgatgttt cggtttgagc gaggaacgtg cgggacgtta actgttactc
    12541 acgccgcccg ccaaccacaa gacaccctcg acctattctt cacaaacggc tccatccaca
    12601 ttcccgtgct gaacgaagga acgatgcgag tgttgacgaa ggacggcgag cgcatcgaaa
    12661 cccacccgaa cccgagtaat attcatcaac cgctcatcga cgactttgcc cgcgctgtct
    12721 tagaaaatcg cgatcccctc gtcaccggcg agatcggacg acgagtcgct tcaacagagc
    12781 aaaagatata caaccaatcc gcgttttgaa aatccgcgtt catccgtgtt tcagtttttt
    12841 acgatcgatc ttcccacgat cgttcttcgg tagcgactca acaaactcca cccaacgcgg
    12901 atacttgtat atcgcaatgt gttccttcac gaactgcttg atctcttcag ctaactgatc
    12961 agcggctgta aatccttccc gcacgacgac gaacgccttc ggcttcacga gcccctctga
    13021 ctcgtggccc acgactgcac attccagcac cgcctcgtgt tgcaacaaac aattctcgat
    13081 ctcagccggc gccacaaaca cgcccgacac tttcaacatg tcgtccgtgc gaccgtgata
    13141 ccagtaataa ccatccgcat cgacgtgaaa ctggtcgccc gaggtacacc agtcgccggc
    13201 gaaagtctct ttcgacttct cgtgcgcgtt ccagtaacag agcgcggctg agtcgccctt
    13261 gattttcagc gttcccatgt cacccgtcgc gacttcgttg tcatcagcat cgacgatccg
    13321 cgcttcgtaa ccctcgacaa tgcggccgag actgccgggc ttgatgtcgc cggggcggtt
    13381 ggtgatgtag atgtgaaaca tctcagcgga accgatgccg tcgcagatat cgatgccaaa
    13441 cgcgttcatc cagcgttcgt aaagctcctt cggcagcgcc tcgccggctg agtaacagaa
    13501 tcgcaggctc gacacgtctt ccgccgaagc gcccgacaca ttcagcatgg aattgatcat
    13561 cgtcggaacg gtggtcagga tcgtcggacg ataacgcttg atgacctcga acatcttttc
    13621 cgcggtcgaa cgttcggaga agagtgccgt agcgccaccg accgcgaaag ggaagagcag
    13681 gttggtcccg gttgcgtagc cgaagaagag tttaggaact gagacggtga gatcgtcagc
    13741 gcagacaccc atcgtcgcct ttgcgaagac ttcggtattg aaaggcagat cgtgttggag
    13801 atggaccgcg cctttcggat gtcccgtcga gccggaagtg aaaagccaga tcgcgatgtc
    13861 gtcccgatgc gtgtccgcgg gttggcattc gtcagaagcg gcgtccacca cttcgtgaaa
    13921 ttcgatccac cttgtgtttt cagggaacgg gtggccccgg agggccaccc ctacagttga
    13981 ttcgtcgcgg tccaaatgac cttcggggtt caattgtagg ggtggccctc cgtggccacc
    14041 cgtgccgaca accaaaacgc tgcgtagata ccgtgcagac gtcgcggctt cggcaaaggt
    14101 gtctagcagt gactcgtgaa caatcgcaac tttcgcgcgt gtgtattcaa ggtaatactc
    14161 gtaatccgca accggcagca gcggattcac catcgtgatc actgctccga tacgcgccgc
    14221 cccaaaccac gtccaaacaa actcgggaca atccggcagc accatcagca cgcgatcttc
    14281 aatgttcacg ccaagctcac gcagcgcatt gcccgtctga ttcgagagtc gtgcgacttc
    14341 gccgtaagtc cactgacgat cttcgtagta aagacagacc ttgttttccc gaccttcctc
    14401 aacattgtga tagaggaagt agtcggccat gttgaaccgc tctggaaatg atggatacac
    14461 gccgaacctc atccacagat tacacagatt ttataaacaa tctgcgtaat ctgtggatag
    14521 acctacgctt ttgtttgacc ctgaaagatt ttttctaaaa atgctgccag cgtggcttcg
    14581 tcttccgggc tgctcgaatt gaacttcaac gcgaaaccga tctccgaagc ttgattaacg
    14641 acctcggccc agaagttgac tgcctgtcga tcggtgagct ggatttcgat ccagacaagc
    14701 tctttcactt caaccttgcc gccggtaagc acgaagcaac cactgcgact gagatcagtg
    14761 acggtcgcat tctcgcgcga gagcacacct tcccagcggg ccgataattt gaccctcgca
    14821 cgcggcgcag atcttctgtc tttctccatc agtgcgcaaa cgggcaacca cgttcaccgt
    14881 gtgtgaggtc gagatgcgtg cgcgcttccc aaagctcggg aaaaaacttt ttgtcgagcg
    14941 tggttttcag atatccgatg ccttccgatc cgccggtgcc gcgcttcgcg cccaccatgc
    15001 gctccaccat ttttatgtga tgcgaacgcc agagcgagaa gtattcatcg tgttcgagca
    15061 ggctttccgc aagctgaaac tcttcgtaac gatcttcgaa atgcgtgaga acctcgagcg
    15121 ccgcgcgcgt gcgttgttca taatcgttcg catcaagacc acgacgttgc agcacacgat
    15181 aaaaacattc tgctaacgtc ggctgcgaca tccgcgcttg caaacgctcg taggcaaact
    15241 catccgtcga aaattcgcgg aggatgtttt tgtctttcag tcccgaagag aactcaatct
    15301 cacgaaactg catcgactga aagccgctcg ccggattcag ttgatcgcga aaggcgagaa
    15361 aactaatcgg cgtcatgctt tcgagaatgt gaatctgatt cacgagcagc ttctcgatct
    15421 ccaccacgcg tttgatcgcg cgcgtcgcgg cgggcacgcg atcttcttcc ataaactgag
    15481 ccgccgcgtc gagctcgtgc aggatctgtt tgaaccacag ctcgtaagcc tggtggacgg
    15541 tgatgaacag cagctcgtcg tggtgcgcgg gttccgacag gcaatcctga agattgatta
    15601 agtcctgaac gcgaagatac ttgttgtaag aaagtggtgg attgtcgccg tagttgttgg
    15661 gcatttagaa cgcagccccc gccgcttcat gttctcgata gctttccgac tcggcaatct
    15721 tcttcatctc gttgatcacg aactccagct cattgtcagt gttatagaag tgtggtgaga
    15781 tgcgaattcc ggctcccggt ctgaagtcga cgataaactc gcgatgaatc agctccttcg
    15841 tgatcgccgc tgcatattcg tgggccaccg tgatcgtccc agcacgttgc tttacgtctt
    15901 tcggactcgt cactgtgaag ccggcttctt cagcgagatt gatcaggtat tccgtttgtc
    15961 gcatgctctt ctcgcggatc ttgtcgacgc cgatcttatt gatgatctgg tagcctgatt
    16021 gcgctgcgta gagcgcggga atcgcgggcg agccgtgcag aaatcgagtg atgtttggcg
    16081 cgtagcgcag cgacgtgtcg aaagcaaagg gcgactcatg ggccatccaa cccgtcgtct
    16141 tcggctcgag tttgtcttgt agatcgggtc tgacgtacaa gtagcccgcg cctggtccac
    16201 cacagagcca ttttaccgat ccgccggtcg cgaaatcagc gttcaactct ttcacactga
    16261 acggcacggt gcctgccgat tgataagtgt cgagcacgac catcgcgccc acttcgtgcg
    16321 cccgatcgat gatggctttt gcgtcctgca aaaacgcact cttgaaaaga acgtgcgaga
    16381 agggaacgag gagggtagtt tcgtcaatcg cggcgagcat tttttcgagc ggcacagtca
    16441 tgccgtcctc tgatttcacc gtctcgatgc gaatgctgcc gtcacgcgcg tgagcctcgt
    16501 agacgtacat caccgacgga aagttcagct ccgaatagac aatcttgttt cgctgcggcg
    16561 tcggattcag acaggaaaga atgagcgact ggcagatcga gacgttctga tgcatcacga
    16621 cgttgccagg atcggcgccg atgatctgcg cgacctcgtc gcccaccgtg acgggcatct
    16681 cccaccagcc ttccgcccac gcgcgcacgc cgcgggtcgc ccacgtttca gcgtagtcat
    16741 ggagccgatc gtaggtcgct ctgggcatcg cgccgagact atgcgagatc aggtaaacag
    16801 ccttatcgag aatcggaaac tcgctgcgcc acttcagtaa atcgtcagac aaataagcac
    16861 cctcctgcct cgtaatttaa cagaattacg cgaatctctg taatccgtgt aatctgtgta
    16921 acggaacatg tggaaattcg ctaatccgat tcgtcacaag atactcgaca cacgctggcc
    16981 caccgctgaa gaagccgcgc gatctcgact gtttagtcca attgctatcg ggaaactcga
    17041 ggctcgcagc cggacgtggg tgcccgcgat ggtgccttgg cgtgcgactg aggatgggtt
    17101 cgtcacgcag cagaatctcg actggtatcg acgttttgcg gaaggacgtc ccggcgtgct
    17161 ggtggtcgaa gcgacgggtg tgcgtgatat accgagtggt ccactgcttc gcattggcga
    17221 cgatcggttc attccgggtt tgcgcaagct tgttgaggtt gtgcgtgaag cgagcggtgg
    17281 cgagactcgt ctgttcatac agatcatcga ctttctcgca gtgaagcgac ggcctgagaa
    17341 ggagaagttc tttcagcggt ttctaacggt tgaggatcat catcgtcagg ccttgatgga
    17401 acttacttca cagtcggcat ggttaagggc tccagacgat cacgtgcgtg agtttttact
    17461 gaatgcggat gaggagatta tcgatctggt gttgcgtccg cgtgagttgg aagcgttgcg
    17521 cttcggatat cgcgagcgcg tttctgacac acacctgaag cacattcgcg atttgccgca
    17581 ggagttgccg gggatatttg cggcagcggc ggagcgggcg cgtgaggccg ggttcgacgg
    17641 ggttgagctt cactacgctc atgcttacac gatggcgggg tttttgagcg cgcagaacga
    17701 tcgtgcggat ggatatggtg gatcgcaaga gaatcgcgtg cggttgccgc tcgaggttta
    17761 tcgcgaggtg cgcaatcgtg ttggcagcga ttacgtcgtt ggtgtgcggt ttctggctga
    17821 tgaagtgatc gcgggtggta gtcgggtcga agacgcgatc ttttatgcga aggagtttgc
    17881 cgcggccggt tttgatttcc tttcgctttc gaaaggcggc aagtttgaag acgcgcaaca
    17941 gccgaaggtg ggccaggctg tttatccgta cactggtcaa agcggttatg agtgcatgcc
    18001 cactgtgctc tccgatgagg ttggtccatt tgggcgcagc gttccgctcg tcgcggctat
    18061 caagaaagcg gtgagcgaga gtggatttgc gacgccgatt gtggccacgg gcggtatatc
    18121 gacatttgag caggcagaag aggttttgcg gctcggtcag gctgatatcg tcggcatggc
    18181 gcgccaggcc ctcgccgatc cggattggtt tatgaaagtg aaactcggtc gtggtgatga
    18241 ggtgcgtcgt tgtacctaca caaactactg cgaagccctg gaccaggcgc ataaacccgt
    18301 gacgtgcaag ttgtgggata aaggccggcg cgtgttggag ccaccaccgt ggtcatccgt
    18361 gtaaatccgt ggctaactga tattcgcgga taaaggcggg atcgcgtctt ttcagatctt
    18421 cataactcac gcgaccgctt ctcgtcatca gcacatacgc aagctcgatc ggactcaggt
    18481 gcatgtatcg agctgcattc tcaaaccagc gcatactctc aaacgctgca gcctggtaat
    18541 cctcgatgat cggccgtcgt gtcttttcaa aatgtgtgag cgcatcggaa acgcttgtcg
    18601 tttgctgaaa cgactccgcc agcgcgatcg catcctcgat cgcgagcttc gtcccggatc
    18661 cgattgagaa gtgcgccgtg tgcaaagcgt ctccaatcaa cacgacattc tcgaaaaacc
    18721 atttgcgatt cttaaccagc agaaagttta tccatttcga attattcgag agcagcttgt
    18781 gcccgttgag atcctgcgcg aagacatcac ctacatagtc cagcgtatcc tcgctgctca
    18841 tctgttcgaa gcccgcgttg gcccacgtct gtggatcgca ttcaacaatg aatgtgctgg
    18901 ttgtgggact gaatttatat gagtgagccg caaacacccc ggcgctattc tcgcgaaacg
    18961 tcaaagttag accgtggaat agctggtgag ttccgtacca gatataacga ttcgcgcgcg
    19021 agtcgagctc aggctgaaac tgctcctggt agcgcagccg gaccgtgctg ttcacgccat
    19081 cagcggcaag caacagatcg caatcgttgc gcagtgcctc gacatcgtga atctccgtgt
    19141 gaaaataaat ctcgacacca agctgctccg cacgtcgctg aagaattttg agtaactgca
    19201 gccgcgcgat ccccgagaag ctattaccat gaatcgaaat cttcgcatcg cggtgaacaa
    19261 catcaacatt gtcccacgcc tcaaactgtt tggtgatctc accatgcgac acatcatcag
    19321 cagcgcgcaa gttggcgagc gtcttccctg aaaaaacaac gccccaccca aacgtatcat
    19381 ccggcccatt gcgctcatag atcctgatct gatgtgccgc atcggccttc ttcatcaaaa
    19441 tcgcaaaata aagtcccgcc ggcccaccac caatgacgtt gatcttcata ttagttgcca
    19501 caaaaagcac aaaagtaaaa gcacaactat ttctcatttt gtgctttttg tgccttttgt
    19561 gtacttgttt tagttctcct ttaagagttg gccggcgatg acgagctttt ggatttcgga
    19621 ggtgccttcg tagatgcgca aggcgcgcac gtcgcggtac atgcgctcga cgactgttcc
    19681 actgaccagg ccggcgccgc cgtgaatttg gacggcactg tcgatgatgc gtccggcggc
    19741 ttcggtggcg aataactttg cttcactcgc ctcacgtgtc acgcggccgg tttcagtatc
    19801 gcgcgcgtaa gccgctcgat aaacgagcag acgcgctgcg tcgagctctg tgatcatgtc
    19861 tgcgagcttt gcctggacta attgatgttc agacaacagg cgaccaaact gtttgcggcc
    19921 ggatgcgtaa cgaactgcct ggtccagggc acgccgcgcc atgccgcagg ccgcggctcc
    19981 aacgctcgcg cgaaacgtgt ccatcgtttc catcgccagc ttaaacccgt caccctcttc
    20041 gccaaccatg tcttcagccg gcactcgaca ctcactgaat acgatctccc cgatcggatg
    20101 cggcgcaatc agcgaggtgc gctcgacaac gcgaaagccc ggcattttcg atccgaccac
    20161 gaatgccgaa agcacaggcc gcccgtcttc acgcgttcct gtctttgcaa acacgatgta
    20221 gaaatcggca atgcctgcgt ttgagataaa gcgttttcta ccgtcgatga cgtatgtctc
    20281 gccatcgtgg cgcgcggttg tctcgatggc ggcgacatcg gatccggcgt ttggttccgt
    20341 gagagcaaag gcggcaatcg ctttaccgtt tgcggcgcgt gaaagccaga agtcgcgaac
    20401 gtgttcgggc gcggcgcgag caatcgcgta cgttcccaaa ccctgcatga cgaaagccag
    20461 atcggcgagt gcggaagagt aagcaagctc ttcgcgaatc aaacagagag aacgaacgtc
    20521 gatcttcgac tcggcgaccg cgtatttcaa aagaccggca cccgccagca gttttacaaa
    20581 atctctggca gcgtcttcaa cattctcttc gacggcagcg cgcggctcga tctcagcggc
    20641 gacgaactgc gcgacgcttt gcgcgagatc gcgatgctcg ggagtgaaga agggcagttg
    20701 agagatcagg cgatccatta ttgaaacctt gcctcgcgct tagcaacgaa cgcctcatat
    20761 gcttcacgat aatccggatg ctgcatgcag atcgcttgtg cctgcgcttc gctttccaga
    20821 gcagtttgaa agttcatctc gagctcgcga ttgagcatct cttttgtctt accgagcgcg
    20881 aacgctggtc cacgcgccag tttctcagca aaggctctcg ttgttgcggc caattcggct
    20941 ggcggcacga cacgattgta aagcccgatg cgttcggctt cggtggcgct cacgaagtct
    21001 cccgtgaaaa gcatctctgt tgctttcgcg agtccgacca cacgcggcag taaataagac
    21061 gctgccatgt cggtgcccgc gagaccaacc tttacaaaca ggaatgcgat cttagcttct
    21121 tcactcgcga tgcgaatgtc agatgcgagc gcgatacacg cgcctgcgcc tgccgtggtg
    21181 ccattgagac tcgcaatgac aggcttcggc agcgcacgca tgttctgaat aagctcgcac
    21241 gtcatgcgtg tgaactcgag caagccttcc atgtcgcgac taaaaagctc gccgatgatg
    21301 tcgtgtacgt cgccacccga acaaaatgct cttccggctc ccgtgatcac tacgacgcgc
    21361 actgcacgtt cgtcccgcag cgcagcaaac gtgtctgtca gctcgcgata gacttcgaac
    21421 gtgagcgcgt tgagacgatc gggacgattg agcgtgatcg ttgcgatgcc tgagtcgtcc
    21481 tgttcgtaaa gaaaagttgt cggttgtttc atgccggtat taccgccatg ccctcgattt
    21541 cgaccttagc ccgatcatcc aaaagcccgg caacctggac gaggaccatc gccggaaagt
    21601 gtttgcccat gtgtttgcga taactgtcgc caacttctct ggtgcgctcg cgatactcga
    21661 tcttgttcgt aacgtatatc acgagccgcg caatgttatc tgcttcacct ccagcggcac
    21721 gcagcaccgt catcacgttt gcgagcgcct ggtcgaactg ctcgacgaag tcgtcgctga
    21781 cgatcttctg gttgttatcc cacgcgattt gtccggcgat aaaaagcagt ttgccgccgc
    21841 cgtcgacgag tagaccgttc gagtaaccac taggcgcacc gagcgcctcg ggattgatta
    21901 acttgaaaga catcgtgagg gacaggttaa cagatctgcg tttatccgcg taaatccgcg
    21961 gctaaatttc tatagcagtt tatcgagacg ggaagctagt tgcgcggcga gattgccgtt
    22021 gggatcgagc ccgccgattg gcccatcaac catgttgccc tgcttgtcga gaatcacgat
    22081 cgacggcagt tggtccacgc caaacttctt cgaaactgtg ccatcgggat ctcgcaacac
    22141 ggtaactttc aaaccatact tgttcgcgaa cgatcgcagt tgttcatcgc tcgcaaagtt
    22201 cttcgacttg gaatcgtctg actcagtgct gacccagtaa acgacaacac ctcggttgct
    22261 gtattcgtct gccagctttc tcacaccctg cagttgggtc ttcgacaacg gcagccacga
    22321 cgcgccaaac gcgagcacca cgatctcgcc acggagactg tcagacgtaa ctgtttgtcc
    22381 atcgattgac cgcagcgaga agtctgcgcg cgcgggattc tgcgcgaggg caacgctggt
    22441 ggccagcaga gtcatgaacc cggttaggac tcgttttcga ctttttcgaa ccatctttct
    22501 ccttcgccga acggctctaa aaacgctcga ctaagtgcgt tagatgaagg cagtcagggt
    22561 tacgttggtc taggcgccga acggcccgta acctccagat gtagggaggt gtaatcatcc
    22621 cacgtatcca tgtcgcgagc aatgaacggc gaggcaaccg ggacgcgtgt gatctcgttg
    22681 cgataggcgt caaaaagcgc tctcatgccg ccgccctccg gcaggttgag taattcagcc
    22741 ttgaaactga gatcgacgag caccgggtga ccgccgcggc cttgccaggt cgggatgaca
    22801 agacgagcgc cgcttttcca tgactcgagg agtgtggaga cgactgtggg cgcgactgcc
    22861 gggtgatcta caagcacgat cagggtcgct tgagctgtct cgggaagctc gcgcacgccg
    22921 gccgcgatcg acgcacccat cggactgtca ggatcgggat tgagtgcgaa cttaacgccg
    22981 gaaacttgtt ggcgaatgtc gtcggcacga tggcccagga cgaccacgat ctcctcaacg
    23041 ccaccagcgc gcaggtaatc gatgcaacac tcgatgacgg ttttgtttcc gaaggggagg
    23101 aggggtttga acgcacccat acggctggag cggccggcgg cgagaagaat ggctgaacac
    23161 gatttcacat gattatcttg tgaaatctta taaatcctgt ctactgctga agatcacgga
    23221 gcgcgcgcag cgtgtactgc cgcgccatct gcttgcgcca ctggtagaca aagtcggtgt
    23281 tgtcgagtgg tcgagctttg atgtaagccg cttcagctgc cgcatggatt tgatcgtccg
    23341 cgagtggctt gccgatcagg gcagtcgcgg cttctttgat ctcgactgga gacggtgcga
    23401 taccgccgag gatgatgcga gcgtcagtaa cggtagtgtt atcagttttg atccacgacg
    23461 caaccccgag cacgggaaag tcaaaagcac cacggcggcg cagtttctgg tacgaagcgc
    23521 gccaaccgtt tgtcggtggt agttgaatat cgacgaggag ctcgtcagga cgtttgtgca
    23581 ggtactcgat gccgtcgtcg ttatacaagc cggcagcgtc gatcacgcgt tcaccaagcg
    23641 ttgaggcgaa tcgcaacttc gcgccgatcg cgaccatcac cggaacgaga tcggatgatt
    23701 ggaccgccca gcacttcgag cttccgggcg cgacccagca gatgtcgcca tccttcttaa
    23761 gacagaagtt gatacctttg cgccactcgt aattctgatc gtagtagttg caacgcgtgt
    23821 cgagcagcag gttgccgccg acggttccca tgttgcggag tagcggcgtg gagatcgtgc
    23881 gcgctgcttt ggcgacgacg ggatagtcgc gattaacgat cgggtgttcg cagatttctg
    23941 tgagcgttac tgacgcgccg atgtgcaagc cgctgttgcc atcgcccgtg atctggttga
    24001 gctccggcag acgcattacc gagataacgg tcctgggcgt ctgttgccgg cgcttcatat
    24061 tcggatacag atcggtaccc ccggcgacga actgacccgc agcccccgcc tcgcccctaa
    24121 tcctcacggc ctccgcgatc gtctgcggaa cgtgatattc aaatttgggc agtcgcatca
    24181 tcgcttacgt tacgctcgtt tcttctcttt tatcttcatc tctcggtgac gagcaagaaa
    24241 tccaccgcgc ttacctttgt agcgaccagg cttgagtgtt gcatcctcgt ctattcccgc
    24301 cgccaggtcg gccaaatagt cttcctctga gatttcaatg gtcacatcct cgccgtcgtc
    24361 ctcgactacc gaaaatacca gaggtctatg ggatggtttt tcttcgttca taaatcttac
    24421 gctctgtttt actagctttc cgtgctgaaa tcagtcgtac gatgtcattt gaccgctcca
    24481 cgtatacgac aaacaacaat cgccgatttg agagcccgat tatgaagaat cgtgtctcat
    24541 cagttgaatg gaatgcgtca tagccttcaa tagcatttgg atcaaagaac acttccttcg
    24601 cttctctgaa gttgacgccg tgcttggcaa aatttattaa cgctttatta gcgtcccatt
    24661 cgaatctcat agtgaggatt gagtccgaaa acctaaaaat ttaattcgtt acttcacgag
    24721 tgtaccgcaa ctcgcggcat ttcgctgccg tctccgcctt cggccggcgt taacactttc
    24781 aggggctcgg gccacgcaac agggggaaca ttgtccgggc cgtagcgtcc ttcgcggcca
    24841 cgcgatttat ctttcagcgc acgcatgacc ttctccggcg tgattgggac ttcgtcaatc
    24901 cgcacgccca cagcgttata aaccgcattc gcgaccgccg gcggcaccgg caacaacggt
    24961 ccctgtccga cttccttcgc accgtatggt ccattcggat cgggatcttc gaccaggtac
    25021 gtcttgacgt cgcacatctc catcgtcgtc gggcttttgt actccagcag cgacgggaac
    25081 ttgtgtacga cgttgcgatt ctcgcgatag accatctctt ccatcaggat ctcgccgaga
    25141 cccatgtaga cactgccttc cacctgccca acaacgagca ccggattgat cgagcgtccg
    25201 atgtcgtgcg cgatccacac gcgctcaacc acgacgattc ccgtatccgg atcgacatca
    25261 acctcagcca ctgcggcgga atagctgtag gccggcgatg gtccaacacc ggcgcctttg
    25321 aacttgcccg gcgatcgcgg cggcgtatac gagccgactg ttccaatcgt cccaaacttc
    25381 gactccgcca gcacgaccgc ttccgcaaac gaaactccga cttccggatt ctcgacatca
    25441 aacacacgtc gctcagccag cgagagattc tcgatcggaa tgccgagttt ttcagccacc
    25501 gccatcacta acaactcacg cgcacgctcg gctgcctgaa tcgcagcatt accggtcatc
    25561 aacgtgaccc gagaagaata actaccaagg tcgaccggcg tcagatccgt gtcagcagtc
    25621 acaacgcgaa tatcaaacgg atcgatcccg agaacttcag cgacgatata agcgagtatc
    25681 gagtccgatc cctgtccgat atcggtcgag ccgcacatca cgcaaacgcc gccttgccgg
    25741 tcgagccgaa gctgcacacc cgactgcggc atgttgttcc agtaaatcgg cagacccgcg
    25801 ccgcagatat acgagctgca agcgatgccg atgcctttgc caaacggcag cttgcgattg
    25861 ttctggtccc acgaaaaacg ctctttccaa cgcgatccct cgacaacttt gtcgatacac
    25921 gcgcccaaac ccatgctgcc gatgcgcagg taattcgccg tcacagaatt cggcgcgacg
    25981 agatgcttcc gacgaatctc gacgggatcg agctgcaact gctcggcgat cttgtcgagc
    26041 tgaacttcca acgcataacg aggctgcggt gtgccgtgcc cgcgctttgg accacacggc
    26101 ggtttgtttg taaacgttcg tattccctga aacttgtaac gcggcacttc atacgtcacc
    26161 gtctgcaacg cacccgtgta gaacgtactc gcgacgccgt aactcccgta cgcaccgcca
    26221 tcgagatacg actgaaagtg catcgccgtg atcgcaccgt cgttcttcac gcccgtcttg
    26281 acgtgcatca acaccggatg tcgtccacga tgacaataaa agacttcctc acgcgtcaac
    26341 gtcaccttca ccggacgccc ggtaatcatc gatagcttgc aaaccacaac ttcgtgatta
    26401 aaaggatcgc tcttgccgcc aaagccacca ccattcggcg tcgcaatcac acgaatgtga
    26461 ctcgccggca aaccgagcac tttcgcgagc gcgcgatgga cgtaatgcgg cgtttgcgtc
    26521 gagctccaga gcgtcatctt cccatctgaa tcgaagtgcg ccaccgcggc gtgctgctcc
    26581 atcggcaagt gggtgttgcc ttcgtagaag aacgtgtctt cacgaacgag atcagcttcc
    26641 gcaaatcctt cttcaaggtt gccgaactcg agcgacacct tcttatgaac gttgccgcca
    26701 tcgccgtaat cgtgaatacg cggctcgtcg atcagcactg cttcttcgat cgtcgagatc
    26761 gtgttgagcg gctcatattc gacttcgatc aagttcatcg catcgaacgc gacgtcttca
    26821 tcgacagcgg caacagccgc gacgggatcg ccgatgaagc gaaccttgtc gacgcagagc
    26881 gcgtgttcgt cctggctgac aggcaggatg ccgtacggaa tcgggaaatc cttcccagtg
    26941 acaacagcaa aaacgccggg caatgccagc gctttcgaga catcgatgct cttgatcaac
    27001 gcgtgtggca gatgcgagcg caggagctta cagaacaaca tgcgcggcag cacgatgtcg
    27061 tccgcaaact tcgtctggcc gacacacttc gcgcgcgcat caactttgcg aaacggcttt
    27121 ccgatgatgt tcaagttttt agccacagat tacacggata aagagcacgg attttagtct
    27181 agtccgtaga cactctcttt gggtaattcc atctcgtcgc cgcggattcg ggcggcagcc
    27241 aattcaacgg cttcgtagat tttaatataa cccgtacaac gacacagatt gccggaaaga
    27301 gcttcctgga tttcagcgcg cgatgggttg ggtgatttct gcagcaactc ttcagcgacc
    27361 agtaagaatc ctggtgaaca atagccacac tgagcggcgc ccagatcggc gaacgtttgt
    27421 tgtagcgggt gtaactccgc gccgtcagcc atgccttcga tcgtcttaac ctcacgacct
    27481 tcagcctcga gaccaagcat caggcacgaa aggatcgagc ggccgtcaac gagcaccgca
    27541 cacgtaccac actcgcccag ctcgcaaccg tgctttgtcc cagtgagatt cagatcttca
    27601 cgcaaaactt cgagcaacgt cttgtgcggt gcgaaggcga cctcggtgtg ttcgccgttg
    27661 agggtgaaat ggatgtgctt cttactttgg agcggcaacg tctttcttct ttactttatt
    27721 caaaatcaca ttatcgatga ggcgcgtctt gcccacgtac gccgcgagcg cgatcagtgt
    27781 cggtcgatcg tcgagccggt cgagtttctc caaggtctcc gcatcagcga tcgtgacgta
    27841 gtctaaacgt gcgcgcggtt cggtttcgac tgtcgagcga attatgtcgg cgagccggcc
    27901 cgcgtttcgc tcaccttctt taaatgcagt cttcgcctgt ttcaaagctt tgtgcaggat
    27961 cgtcgctgag ttctgttcgt ccgcagtcag atagagattg cgcgacgaaa ttgcgagtcc
    28021 tgaatcctca cgcacgatcg gcaagatgac gatctctgta tcgaacgcga gatccttgac
    28081 gagacgcttg atcacaagcg cctgttgtgc atccttttga ccgaagaaag cgaagtcagg
    28141 ccgcaccgta ttgagcagga cagtgacgac ggtcgcgaca ccgcgaaagt ggcccggtcg
    28201 tgaatgtcct tcgagctgct cggaaagtcc ttcaaccttc acgtaagttg aaaaaccttt
    28261 cggataaatc tcttcgacag tcggcgcaaa gatgtaatcg acgttgtaat cagtcagcag
    28321 cgcggtgtct ttggttagat cgcgcgggta gtgctcgaag tcttcgcctg gtccaaactg
    28381 cgtcggattc acaaaaacag aaaccaccac gacgtcgcac atccgtctcg cttcgcgcac
    28441 gagactcagg tgcccttcgt gcaaggcgcc catcgtcgga accaatccca cagttttatc
    28501 ctgttcacga cgaattttgc gcgcgacgga actcatgcgc tgacgacgat taatgatctc
    28561 catagttgag gttgagtttt aggacttctc ggcctttgag tctctcgatg ggacctcgat
    28621 atccagctct gccgcagttt gtgcgggcag tgcatacgat tctgcttccg aggggtaagt
    28681 gccgtttctc acgtcttcag cgtatcgcgt gatcgcgtcg gtcatcacct cgcgcaagtt
    28741 tgcgtaaggc cgcacgaagc gagcttgttt gccaaacgac aggcccagca tgtcgtgtat
    28801 gaccagcact tgaatatcgc agtgaacacc cgcgccgatc ccgatcgtcg gaatgctgat
    28861 gctctcggtg atcatttggg ctatctcgcg cggtacgact tcaagcacaa cggcaaacgc
    28921 gcctgcgtct tccattgctt tggcatcgtc gatcaactgc tgcgccgcgc tcgcagtctt
    28981 gccctgcacg cggtaagcgc cgagttgatt gatcgactgc ggggtcaggc cgatatggcc
    29041 catcacgctg atctcttcgt ccacgaggcg cttcacgagg tgcacgcgct tgtgtccgcc
    29101 ttcgagcttg attgcttccg cgccgccttc cttaacgagt cgcagcgcgt ttctcacggc
    29161 gtcgtcatcg cccgtgtgga aagtcccgta cggcatgtcc gcgacgagca aggctcggtt
    29221 gacggcgcgg cgcacggctt tcaaatgaat gagaatctcc tcgagcgtca cgggcaccgt
    29281 gttgccataa ccgaggacga cattcccgag gctatctcct acgaggatga tatcgattcc
    29341 ggcttcatca acaatgcgag cggtcgggta atcgtacgcg gtcagacaaa cgagtcgttc
    29401 gccacgttgt ttacttgacc gcaaagacgg tgcactgacc ttttccggcc gttcaggctt
    29461 cagatagggc atgattcagc tctctttaca ccacgatttg tggacaattg aattgccagt
    29521 ggaacaggag acttgcagtt gtcgcgggcg tgataacaag gcgggcatta taacgggaaa
    29581 cgcggaaacg gggaaagcgt ggacaggatt acaggattta caggattaac aggatttgtc
    29641 ttgtcaatcc tgtaaatcct gcaatcttgt ctaagccttc caccgcacca cactggacga
    29701 gtctttcgtt tcggccagca actcgcggat tggcactccg agcacgagat ttgggacgag
    29761 ctcggcgagc ggcacgagca cgaagcgtcg cgaggcgatc cgcgggtgcg gcagtttcaa
    29821 aaactccgta tccgatcgcg cgtctttaaa tatcaacaaa tcgagatcga tcgtgcgtgg
    29881 acccatccgc acatctcgtg ttcgtcccag cgcatactcg atgcgcaaca accgcgccag
    29941 cgtctgttca ggcgagggaa gcgtcgagcc acgcagctcc gccaccatgt tgagaaaggc
    30001 cggctgctct tcatactcca ccgcttcggt ttcataaatg ctcgagagtc gaataacatc
    30061 caatccggcg tccagcatgc cacgcacagc cagcaacagg tagccggcgc gatcgccgag
    30121 attggagccg agtccgacgt aagcgttctg gatcattaag cgatgtccca attactgacc
    30181 ttaatctggc ttaaatggcg actgttgcgc aactcgcttc gctcctcgaa agccgtcgtc
    30241 aatcgcgcgg cctcgattct cggtatgctg atcgcgctgt ttctggcact catcgtagcc
    30301 ctcatactcg gcttcgccgc ctacggactc agccagcctg aagcgcttgg taacgcattc
    30361 agacgaacgg ccacacgcga tgtttctgcg tcggcatcgg cggagttcat cttcttttcg
    30421 atgttcgggc tcatttacct gatgtgggcc actgtgcctt tgagtatcgg cggcggcaag
    30481 cagtttgacg cgggcaagat gctgatgtat ccgatcacgc ttcgcaagct ctttgccgtc
    30541 gacttcctca gcgagctgac aacgctgcac tcggtgtttg caataccggc tgtgatcgcg
    30601 atctgcgcgg gtgtcgctct cggcaccggc cgtacgacgg cggctgcagt ggcggcagtt
    30661 ccagcgatac ttttcggcgt cgcgctttcg aaatggcttt caacaacgat cggatcactt
    30721 ttgcgtcgca aacgtgcgcg cggtgaaacg ctcgtggcgc tgatcggcgc tgtggcaggt
    30781 ttgggcgcag cagtccttgg tcagatcgcg ccgatactct tcacacacgc cgagtcgttt
    30841 cgatcgttgc gctggacgcc gcctggagct gcggcgtact tgttagtcgg tcctccaaac
    30901 gacgtcgccg gatatgtggt cgctttcatc acgctgagcg cttatggcgt ggccttggtt
    30961 attgcaactt attggatcgc gcgtcgcgcc gcactcggaa tcgagggacg tcgccgacaa
    31021 aagacagccg tcgttgccga gacggcgacc ggttactccg gctggcaatt gccgctcgtc
    31081 tcaccggagc tttcggcaat tgtcgagaag gaagtgcgct acgcgatgcg caacgcacag
    31141 gtacgaatga tggcgctgat gcctctggta ctcctcgtcg ttcgcgtcat gaacacaaaa
    31201 aagtggtggg gaactgcctc accatcgggt agttttctca cttacggttc gggtttgctt
    31261 gcaactggcg gcgtgctcta cgtctttctc atcctcgcgg gattgtcgtg caatcacttc
    31321 gcgttcgagg aaggtgggat gcgaacgttg atcctgtcgc cgatcgatcg tcgcaagata
    31381 ctgctgggga agaacatcgc gatcacactg ttggccctca tcttcgcaac gatcctgttg
    31441 acgctgaacg cgcttgtctt tcgcgatttc gaagcgtcga cattgttgtt cgtcggtttg
    31501 agttttgtga actttgcggc gctcagcgcg atgatgggca actggctgtc gattcgtttt
    31561 ccaaagcgca tgcggtttgg aaagcgattg aatgtgtcgg gcgtggcggg gctgttgttg
    31621 attccgatgg tgatcgtgct cggcgcgccg ccggtgctgg ctacactcgt tggtttgttt
    31681 atgagcagtt tcttgtatga gtatctggcg ctgttattgt ttgcgctttt atcgattggg
    31741 ttctatctcc cgatgctcaa tcttcagggt agatcgcttg cgaagcggga gatcgatatt
    31801 ctcgaagcag tacgcgagcc ggcagacgag taagagtgtc cacagattac acagattcag
    31861 agaaagtctc tgtgtaatct gtggatggtt tttgttacac tacgtgcccg gtactttgaa
    31921 tctaattcca gtgatgccgc ggcatccggg cgggttttga gccccgtcaa tacacaaatg
    31981 gctcgcagaa gatttcgcag caaccagcgg cacgaaccca ccacggagcg ttcctttcag
    32041 gaatacatcc tttccactcc cgcaccacaa acatctcgca ccgagctgtt gcgactgatc
    32101 cgcggtggtg aagacacgtt tctcgagctc aaggtcaaac tctcaaacac agaaaagatc
    32161 gcgcaggaga tcgtagcgct cgcgaatacc ggcggcggcg tgctcgtctt cggtgttaac
    32221 gatcaactgc gagttgaagg ggttgacgat ccggagcgag ttcaggaaga cctcgtgcga
    32281 atctgtcgtg aagaggttca gcctccgctc gtcccgttta tcgatcgcat cgcactcgac
    32341 aacggtcgac gcatcgtcgc gcttgatatc gaaagcaagc gacgcccgta tcgaacacgt
    32401 gatggtcgtt tctacctgcg catcggagca gagaaacgcg aggcgtcgcg tgaagagatc
    32461 tcctcgttat tagaagagtc gcgcccactt cgctatgaag atgtacctgc gctgggctcg
    32521 acgatggacg acatcgacga ggcgcatttg tggagctacc tgcgcgagtt tgaagggggc
    32581 gcgtttgaag aggccacggt ggttggttat ccgactgctg acgtgctcga acgagatctg
    32641 ttactggcga cgaatgatgg aaacgacatc gtgccgactg tcgccgggtt gttgctgttt
    32701 ggacgtgatg agcgagtgac ggagttgttg ccgcgcgcgt ctgtgacggc gacgcggttc
    32761 tccggtgatt cgctgcagtc acccgtggtt gaacgagtca aattctcggg aaacctggtt
    32821 tcgatttacg aagggctgat gcggttcgtg cgtcgctatt gcgatcttgc ggaagtgcgt
    32881 ccgaagagtt ctgccttaac agatgagtcg cccgtgccgg cgcgtgcgag ttatcacggc
    32941 ggtgttgtgg ctgaggcaat cacaaacctg ctggcgcacc gcgatctcgc tttgagagat
    33001 ctgccgagcc gtctgaatgt gtttgatcac tcattggagt ttgttaactc gcgacgcagt
    33061 gtcgggttct cgccggttgc gcagaaggcg atacgttatg gaat
//
LOCUS       DASSFQ010000125        10563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1689740, whole genome shotgun sequence.
ACCESSION   DASSFQ010000125 DASSFQ010000000
VERSION     DASSFQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10563
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689740"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFH96_04310"
     CDS             <1..243
                     /locus_tag="VFH96_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973220.1"
                     /translation="SYGVITGDGSAFLAVNLLFQDLLPEGSMVGDGVITGDGVITGDG
                     VITGDLVASAQSAMLNGEGSSTMAAPSDDGNDCLDY"
     gene            327..2297
                     /locus_tag="VFH96_04315"
     CDS             327..2297
                     /locus_tag="VFH96_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973221.1"
                     /translation="MSAAEVDVNRPQFGQVYMWSLVIAGAAIVLVSLYQLPYGDIDPR
                     FGFLCLMVMASALIAIPIPRVSGRITVADTFVFLTMLLYGGPAAVLVSALEGVAATLI
                     ISKRPRTILFNSAILATSTFCTATVLHLKFGSITAISQQFTQNFFIAVCLMALVQYIA
                     NTVLIAIEKASKIKESVWNTWRTYYLWTSVTYFAGASAAGIIAILINRYGFYAVVATA
                     PIILIICFTYQTYLKNIEASLEQTEAARLHVEELSRYISQLQRSEDARGQLLLRAERA
                     RADAEAANRIKDEFLATLSHELRTPLTSLLGWSSVLREGKRDEKVLAQGLDAIDRNAR
                     VQAQLIDDLLDVSRIVSGKLNLDVRPLDIASVARAAINVVQPAADAKGITLDYYAEPG
                     LGAISADSARVHQIVWNLLSNAVKFTPHGGKISLRVEQDETDARVTVQDSGQGIDPEF
                     LPRVFDRFRQADSSTTRSFGGLGLGLAIVRHLVELHGGTVSAQSDGVSKGATFTVTFP
                     LMADRTPVTMLHTPEISDTYSLDGLKVLLVDDEPEARQIISTVITRTGAEVKSCKSAR
                     EALVKLSEWKPDVILSDIAMPEQDGYSFIRQVRSLPRDKGGDTPAAALTAYARDIDRR
                     QALAAGYQMHIAKPIGASQLVTMIARLAGRDS"
     gene            2385..3482
                     /locus_tag="VFH96_04320"
     CDS             2385..3482
                     /locus_tag="VFH96_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6973222.1"
                     /translation="MKRSPVGIGIIGSGFARTTQIPGFRDCMGAKVVAVASRNRDRAE
                     TIAKEFGIEHVASDWHELVAHPDVDLVSIVTPPSTHMEIALAAIEQRKAVLCEKPMAL
                     NAGEAARMVEKAKAAGVLALIDHELRFLNSRRVMRGMLQTGAIGSVRHCNYVFRSDYR
                     GIARPWDWWSDEKMGGGALGAIGSHVVDSFRWTLSAEVTKVLGMLTTHIGQRPDKTNG
                     GGMRAVTTDDEAKLLFRFSDGPHTENTTGAASISVVESGKYENRLEIYGTKGALMVEE
                     TGELWLSPTGSGTWRPVQVEQDHMAQGMREGSWSRGFTAFSIAICDALRAGKATVKDA
                     ATFEDGYRVQLALDAIRASNESGCWVTVSQT"
     gene            3532..5556
                     /locus_tag="VFH96_04325"
     CDS             3532..5556
                     /locus_tag="VFH96_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET6973223.1"
                     /translation="MLILCYNSRRKSIRIGVDTGGTFTDFVFERDGRVQLFKLPSTPS
                     DPSLAIQQGLARICAETGAQLSKVEVVHGTTVGTNALLQRRGARIALITTRGFEDVLV
                     IGRQARPELYNLNAIKPASLVDDDLRLGVRERVVASGEVIEPLDEDDLRRIVKKLKHV
                     EAVAISLVFSFLHPEHEERIATALAGLEVPLSISSRILPEYREYERTSTVVINAYLQP
                     LMGRYLKRLSGENRGLNLRVMQSSGGSISADVAAVEPVRTILSGPSGGVVGALRAAQS
                     AGIQNVITFDMGGTSTDVALCDSGAIRTTNEAIVAGLPVAVSVMDIHTVGAGGGSIAR
                     VDEGGSLRVGPESAGADPGPACYGRSLLPTVTDAHVVLGHFGGLGLLGGEFKLDEKRA
                     HEAMTNLANDLSKAAGKRCSAIVAAEGVLSVANTNMERALRHISVERGHDPRQFALLP
                     FGGAGGLHAVDLARALRIPKIIAPTAPGALSAVGVLVADVIKDQSRTVMFTLKQISKL
                     GKVFTEMEREATVLLRSEGFPRSRQRHERSLAMRYRGQSFELEVRSLSGDLAEEFHRV
                     HRERYGYAQEQSEIEIVSARLRSFGVVEKLPLTKIGSGKVKAHGEVNMYLYGRKTTAA
                     LYRREELFANTKLMTPCLVTEYSATTLIDADSKARIDGFGNLLIEFKNEV"
     gene            5546..7117
                     /locus_tag="VFH96_04330"
     CDS             5546..7117
                     /locus_tag="VFH96_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET6973224.1"
                     /translation="MKFDPTTLEIYRALFTSVAEEMGVALRRTAFSPNIKERRDYSCA
                     VFDADGRVIAQGDHMPVHLGSMPMAVAAALAEIDLVEGDVVALNDPFAGGTHLPDVTL
                     VMPVVSEKRTLFYVANRAHHADIGGATPGSMGLATDIYGEGIRIPPIRLVRKGVLDSD
                     TMRLLLANVRGNVERRGDFDAQIGSLKTGASRLLEIVERRGEGEATEYASQLINYSAR
                     LMRHTIAAIPDGVYQAEDALDDDGVEDRPVPIKVRVTIKGERAVVDFTGSAPQVVGAI
                     NAVEAITVSAVSYVFRCLVGGEVPASAGLMEPIAVIAPRGTVVNANPPASVAGGNVET
                     SQRIVDVLFKALAQAVPDRIPAASQGTMNNLTIGGIDTRTGLEFSYYETVAGGMGARP
                     SADGMSGVHTHMTNSLNTPAEALEYAYPLRVREYRLRRGSGGRGKHRGGDGVVREIET
                     LVAARMSLLCDRRKRGPYGLFGGEDGAAGINELISQKKKSKLPSKGSHELKAGDRIRI
                     ETPGGGGFGNADDAD"
     gene            complement(7165..10224)
                     /locus_tag="VFH96_04335"
     CDS             complement(7165..10224)
                     /locus_tag="VFH96_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973225.1"
                     /translation="MAEDQHSAERQSFLELTRSLTGLPLDQAAAVLETSASIAAISLR
                     AGIEFLRTAPAAAHVLQPTELRAWGEMGRRLAMTDYESAATFFAEGVEALRTVTPELR
                     PSIFVLCSRQMVLSTAIGRETLHSFPGLAAEIDDDELLRAILDVATEIARRSAKHSAE
                     FLNAGSTVSRALKSFQDPAVTAAALGLSGAFAARAGGIAADAWAITPEAIADLSAADA
                     IRLLKTASSFLERGGGAALHVLIAGGEILRRAPEIFDEWIALLWVVAMHGNASLVAFI
                     RSSPGFIRILNADPNRAAAIALSHRVLALTKEIAAVDGEAALACFRSSARALRSVSIE
                     QFEAWARRGLTSGRTDTRARRSYFSLETRGSYEALLSGSAGLALESIQHLLRLYVEAL
                     TGREVEIAPLAAVPDEARIDDGRTIHLPSLVNEFGDEELDFRLYKVLAAHAAGQIEFG
                     TYERGGMLRVAHSAIAAKYSKENVDALDAFAIADEIVQLRADEEGSHEKAQEHTEDIG
                     YRDVLKAFPLPQLARRVFGTLENGRIDRRLRRAYRGLSRDLDLIRQHLSQSRPSITGL
                     PGALVPFELLFQITMLGGAREDSRLAYGQIVSELETVVSEYLLREHMTVADTLMATSR
                     VYDLFQTVMPVDDSVQQVEAPEDQTESDDDSSASDRMKQRQAERTPERRDARELFNAW
                     NDPSAESDPDELTGAEAWSEAETPEQVLAEGEVAYNYDEWDRELTDHRLGWCRVIEKK
                     VKHGDRNFVDLTKERHKGVISSIRHQFQLMKPENLTRVANEVDGEEFDLNAVIDFVID
                     RRADGYQSERIYTKRLRRRRDVAVSFLLDQSSSTARTIGRHPLQPYTRPGRRIIEIEK
                     EGLVLMSEALEAVGDTYSINGFTSEGRRNVKFYVVKDFDEKYSDEVKRRIGGISYQNN
                     TRLGAAIRHASAKLAKQDARTRLLIVLSDGRPYDHDYGDARYAREDTREALRQAKNEG
                     ITPFCITIDRESESELRDLYGEVGYTIIDDVLSLPERLPGIYRRLTT"
     gene            complement(10261..>10563)
                     /locus_tag="VFH96_04340"
     CDS             complement(10261..>10563)
                     /locus_tag="VFH96_04340"
                     /inference="COORDINATES: protein motif:HMM:NF020002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ C-terminal domain-containing
                     protein"
                     /protein_id="HET6973226.1"
                     /translation="RFVAIEFDYPPPEMEAEIVAREGGVDEGTARDLVLIGQKVRNLR
                     GHGLEEGVSTRLLIYAAQLIALGIPPVDAADVAMCSPITDDRELQRSIREIVTTVI"
BASE COUNT         2193 a         2881 c         3214 g         2275 t
ORIGIN      
        1 agttacggcg tcattacggg agacggttct gcctttctcg cggtaaatct tttgttccag
       61 gatctactgc cggagggtag tatggtggga gacggagtaa taaccggaga cggcgtcatt
      121 actggcgacg gcgtgatcac aggcgacctc gtagccagcg ctcagtcggc catgcttaat
      181 ggcgagggta gttcaacgat ggcggcacca tcagacgatg gcaacgactg cttagattat
      241 taaactgatc cacagtttac atagattgca ctgatcacac ggccaatccg tgtgagcggc
      301 gtgttctgtg gctacgaggt aggactatga gcgcggcaga agtagacgtt aatcgacctc
      361 agttcggaca agtttacatg tggtcgctgg tgattgcggg cgcggcgatc gtgctggtct
      421 cgctctatca attgccatat ggcgacatcg atccacgttt tggattcctg tgtctgatgg
      481 tgatggcgag cgcgcttatc gctattccca tcccacgcgt gagcggtcgc attacggtag
      541 ctgacacttt tgtctttctg acgatgctgc tctacggcgg tcctgcggca gtactcgtct
      601 cagcgctcga aggcgtcgcc gcgacactga tcatcagcaa acggccgcgc acgattcttt
      661 tcaactcagc cattctcgcg acgtcgactt tctgtactgc gacagttctt catttgaaat
      721 tcggatccat cactgcaatc tcgcagcagt ttacgcagaa cttctttatc gcggtctgtt
      781 tgatggccct cgtgcagtac attgccaaca ctgttctgat cgcgatcgaa aaggcgagca
      841 agatcaaaga gtcagtctgg aacacctggc gaacttatta tctctggact tcggtgactt
      901 actttgctgg tgcgtctgcg gccggaatca tcgcgattct catcaatcga tacggcttct
      961 acgcggtggt tgcgactgct ccgatcattc tcatcatttg tttcacctac cagacttatc
     1021 tgaagaatat cgaggcgtcg ctggaacaaa ctgaggcggc cagactgcac gtcgaagagt
     1081 tgtcgcgcta tatcagtcag ttgcaacgtt cggaagacgc ccgcggccag ttgctgctgc
     1141 gtgctgagcg tgcgcgtgct gatgcggaag cagcgaatcg aatcaaagac gagtttctcg
     1201 cgacgctctc gcacgagttg cgaacgccgc taacgtcgtt actcggctgg tccagcgtac
     1261 tgcgtgaagg caagcgtgat gagaaagtgt tggcgcaggg gctcgacgcg atcgatcgca
     1321 acgcgcgtgt gcaagcgcag ctcatcgacg atctgctcga cgtgtcgcgt attgtttccg
     1381 gaaaactcaa tctcgacgtc aggccgttgg acatcgcgtc tgtggcgcgg gcggcgatca
     1441 atgtggtcca gccggcggct gatgcgaaag gcatcacgtt ggattactac gccgaacccg
     1501 gactcggcgc tatctccgcc gactccgcgc gcgtgcacca gatcgtctgg aaccttctgt
     1561 cgaatgcagt gaagttcact ccgcatggcg gcaagatctc tttacgcgtc gagcaggacg
     1621 aaacagatgc ccgcgtgacg gtgcaagact cgggtcaggg tatcgatccg gagtttctgc
     1681 cgcgagtatt cgatcgcttt cgtcaggcgg atagttcgac cacgcgaagt tttggcgggc
     1741 tcggactcgg cctcgcgatc gtgcggcatc tcgtagagct gcatggcggc actgtttcgg
     1801 cgcaaagtga tggcgtaagc aaaggcgcga ctttcacagt tacgtttccg ctgatggcag
     1861 atcgcacgcc ggtaactatg cttcacaccc cggagattag tgacacttat tcactcgatg
     1921 gcttgaaagt gttactcgtc gacgatgaac cggaagcacg ccagatcatc agcaccgtga
     1981 tcacacgcac tggcgctgaa gtgaaaagct gcaagtctgc gcgtgaagca ctcgtgaaac
     2041 tttcagagtg gaagccggac gtgatcctga gcgacatcgc gatgccggaa caggatggct
     2101 acagttttat ccggcaggtg cggtcactgc cgcgcgacaa aggcggcgac acgcccgctg
     2161 cggctctgac tgcgtatgcg cgcgacatcg atcgacgcca ggccctcgcg gcgggctacc
     2221 agatgcacat cgccaaaccc atcggcgcca gccaactcgt gaccatgatc gcccgcctcg
     2281 ccggccgcga ctcttaaaaa ctttatccac agatttcaca gattgctcag attaaatttc
     2341 aggtctgtgc aatctgtgta atctgtggat aatataatag ggtcatgaag aggtcaccgg
     2401 tcggaatcgg aatcatcggc tcggggtttg cccgcacgac acagatcccc ggctttcgcg
     2461 attgtatggg tgcgaaggtg gttgcggtcg ccagtcgcaa ccgcgatcga gctgaaacga
     2521 tcgcgaaaga gtttggtatc gaacacgtcg ccagcgattg gcacgagctg gttgcacatc
     2581 cggacgtcga tctcgtcagc atagtcacgc ctccatcaac tcacatggag atcgctctcg
     2641 ccgcaatcga gcaacgcaaa gcagtccttt gtgaaaaacc gatggcgttg aatgccggtg
     2701 aagccgcgcg catggtcgag aaggccaaag ccgccggcgt gctcgcgctg atcgatcacg
     2761 agctgcggtt tctgaacagc cgccgggtga tgcgcgggat gttgcaaaca ggcgcgatcg
     2821 gatcggtgcg tcactgcaat tatgttttca ggtctgacta tcgtggcatc gctcgtccct
     2881 gggactggtg gtcggatgaa aagatgggag gcggtgcgct cggcgcgatc ggatcgcacg
     2941 tcgtcgattc ctttcgctgg acactgagcg cggaagtcac gaaagtgctc ggcatgttga
     3001 cgacgcacat cggtcaacgt ccggataaga cgaacggcgg cggaatgcgt gctgttacga
     3061 cagacgatga agcgaaactc ctgtttcgtt tcagtgatgg tccacatacg gagaatacaa
     3121 ccggcgcggc ttcaatctcg gtagttgaat cgggcaagta cgaaaatcgg ctggagattt
     3181 acggcaccaa aggcgcgtta atggtcgagg agaccggcga gctgtggctt tcgccaacgg
     3241 gatcaggcac gtggcggcca gtgcaagtcg aacaggacca catggcccag ggtatgcgcg
     3301 agggaagctg gtccagaggc ttcaccgcat tctcgattgc gatctgtgac gcgttgcgag
     3361 caggcaaggc aaccgtcaaa gacgcggcga cgtttgaaga cgggtatcgc gtgcagctcg
     3421 cgctggatgc gattcgcgcg tcgaatgaaa gtggctgctg ggtgacggta agtcagacgt
     3481 agacaggatt aacaagatgt ttcaggatga acatgtcaat cctgaaacat cttgttaatc
     3541 ctgtgctaca actctcggag aaaatccata cgtatcggcg tcgacacggg cggaacgttt
     3601 accgatttcg ttttcgaacg agacgggcgc gttcaacttt tcaagcttcc ctcaactccc
     3661 tccgatcctt cgctcgcaat tcaacagggc ctcgcgcgta tctgcgctga aaccggcgct
     3721 caactctcaa aagtcgaggt cgtacacggc acgactgtcg gcacgaacgc cttgctgcaa
     3781 cgccgtggcg cgcgcatcgc gctgatcacg acgcgcggtt ttgaagacgt gctggtaatc
     3841 ggacggcagg cgcgtcctga gctctacaat ctcaacgcca tcaagccggc gtcgctcgtc
     3901 gacgatgatc tgcggctcgg cgttcgcgaa cgtgtagtgg cgtcaggcga agtgatcgag
     3961 ccgctcgatg aagacgacct gcgccggatc gtgaagaagt tgaagcacgt ggaagcggtc
     4021 gcgatctcgc tggtgttttc ttttctgcac cccgagcatg aggagcgtat cgctacggcg
     4081 ctggctgggc tggaggtgcc gttgtcgatc tcgagtcgca ttctgccgga atatcgcgag
     4141 tacgagcgca cctcgacggt cgtgatcaat gcgtacctgc aacctctgat gggccggtac
     4201 ttgaaacgac tgtccggaga aaatcgaggc ctcaatcttc gcgtgatgca atcgtccggt
     4261 ggcagcatct ctgcggatgt tgcggcagtc gagccggtgc gcacgatttt gtcaggaccc
     4321 tccggcggcg tcgttggtgc tctgcgagcg gcacagtcag ccggaattca aaacgtgatc
     4381 acgtttgaca tgggcggcac ttcgactgac gtcgcgctct gcgacagcgg cgcgatacga
     4441 acgacgaatg aagcgatcgt tgccggactg ccggtcgcgg tttctgtgat ggacatccac
     4501 actgtcggcg cgggtggtgg atcgatcgcg cgcgttgatg aaggcggctc gcttcgtgtt
     4561 ggaccagagt ctgcgggcgc cgatcctggt ccggcgtgtt acggacgctc gttgttacca
     4621 accgtcacgg acgcacatgt cgtattgggc cacttcggcg gtttgggttt gctcggtggt
     4681 gagttcaagc tcgacgaaaa gcgcgcgcac gaagcgatga cgaatctcgc gaacgatctg
     4741 agcaaggcag caggcaagcg gtgcagcgcg attgtagcgg ctgagggcgt gctgtctgta
     4801 gcaaacacaa acatggagcg cgcgctacga cacatttcgg tcgagcgcgg acacgatccg
     4861 cgacagtttg cgctcctgcc gttcggcggc gcgggtggtt tgcacgccgt cgatcttgcg
     4921 cgtgcgttgc gaattccaaa gatcatcgcg ccaacagctc cgggcgcgct ttcggcagtc
     4981 ggtgtgctgg ttgctgatgt gatcaaggac cagagccgca ccgtgatgtt tacactgaaa
     5041 cagatcagca agctgggaaa agtgtttact gaaatggaaa gggaagcgac tgtcttgttg
     5101 cgcagtgaag gtttcccacg ctcacggcaa cgacacgagc gttctctcgc gatgcgttat
     5161 cgcgggcagt cgtttgagtt ggaagtgagg agcttgtcag gtgatctcgc ggaggagttt
     5221 catcgtgtgc atcgtgagcg ttacggttat gcgcaggagc agagcgagat cgagatcgtg
     5281 agtgcacggc tgcgatcgtt tggagtggtc gagaagctgc ccttaacaaa aattggatcg
     5341 gggaaagtaa aagcgcatgg ggaagtgaat atgtacctct atggccggaa aaccactgcg
     5401 gccctctata ggcgagaaga actgtttgca aatactaaat tgatgacacc gtgcctcgtg
     5461 acggaatact cagcgacaac actgatagac gcggattcga aagcacggat tgacggcttt
     5521 ggcaatttac tgatcgagtt caaaaatgaa gtttgatccg acaactttag aaatttatcg
     5581 cgcgctcttc acttcggtgg cggaggagat gggtgtcgca ctgcgtcgca ccgcgttctc
     5641 gccaaacatc aaagagcgcc gcgattactc gtgcgcagtg tttgatgctg atggtcgcgt
     5701 catcgcgcag ggcgatcaca tgccggtaca tctcggatcg atgccgatgg ccgtggccgc
     5761 cgcgctcgcg gagatcgatc tggtcgaagg tgatgttgtc gcgcttaacg atcctttcgc
     5821 aggcggcaca cacttgcctg acgtgacgct cgtgatgccc gttgtctccg agaaacgcac
     5881 gctcttttat gttgcgaatc gcgcgcatca cgctgatatc ggtggtgcga cgcctggctc
     5941 gatgggcctc gcgaccgaca tctatggcga aggcatacgg atcccgccga ttcgtctcgt
     6001 acgcaaaggc gttctggata gcgacacgat gcgtctgttg ctcgcgaacg tccgtggcaa
     6061 cgtggagcgt cgcggcgact tcgacgcgca gattggctcg ttaaaaactg gcgcatcgcg
     6121 actgctcgag attgtcgagc gtcgcggcga aggtgaagca accgaatatg catcgcaact
     6181 gatcaactac tctgcgcgct taatgcgaca cacgattgct gcaattccgg acggcgttta
     6241 ccaggctgaa gacgcgctgg acgatgatgg tgtcgaagat cgaccagtgc cgatcaaagt
     6301 gcgtgtcaca atcaagggcg agcgcgcggt cgttgacttt acggggagcg cgccgcaggt
     6361 cgtgggcgcg atcaatgcag tcgaggcaat cacggtttcg gcagtaagtt atgtgtttcg
     6421 ctgtttggtt ggcggtgagg tgcccgcgag cgcgggattg atggagccga tcgcagtgat
     6481 cgctccgcgc ggcacggtag tgaatgcaaa tccaccggcg tctgttgcgg gcggcaatgt
     6541 tgagacgtcg caacgcatcg tcgatgtgtt gttcaaggcg ttggcccagg ctgttccgga
     6601 tcggataccg gcggcgagcc aggggacgat gaacaacctg acgattggcg gtatcgatac
     6661 gcgcaccgga ctcgagtttt catactacga aaccgtcgcg ggcggaatgg gtgctcgtcc
     6721 ctcggctgat gggatgagtg gcgtacacac gcacatgacg aacagcttga acacgcccgc
     6781 tgaagcgctg gagtacgctt atccgttgcg cgtgcgtgag tatcgactgc gcaggggttc
     6841 agggggacga ggaaagcatc gtggcggcga tggtgtcgtg cgtgagatcg aaacgctcgt
     6901 cgcggcgcgc atgtcgttgt tatgcgatcg acggaaacgt gggccatacg ggttgtttgg
     6961 tggagaggat ggagcggctg ggatcaatga gctgatttct cagaagaaaa aatccaagct
     7021 tccatcaaag gggtcgcatg agctcaaagc aggggatcga attcggatcg agacgcccgg
     7081 tggcggcggc tttgggaacg cggatgacgc ggattgaaaa cgcggatttt actcagatct
     7141 gcgaagatcc gcgttaatcc gcggctaagt agttagtctt cgataaatcc cggggaggcg
     7201 ttcggggagg gagagaacgt cgtcgatgat ggtgtagccg acttcaccgt aaagatctcg
     7261 gagctcggat tcggattcgc gatcgatggt tatgcagaag ggtgtgatgc cttcgttctt
     7321 tgcttgtcgc agagcttcgc gagtgtcctc gcgcgcgtag cgtgcatcgc cgtaatcgtg
     7381 atcgtagggg cgtccatcgg aaagcacgat caataatctt gtgcgggcgt cttgtttagc
     7441 gagcttcgct gaggcgtgac ggattgcggc gccgagtcgc gtgttgttct gataactaat
     7501 gccgccgata cgtcgcttta cttcgtcgct atatttctcg tcgaaatctt tgacgacgta
     7561 aaacttcaca ttgcgtcgtc cttcagaagt gaatccattg atcgagtaag tgtcgccgac
     7621 ggcttcgagg gcttcgctca tcagcacgag tccctctttc tcgatctcta tgatgcgtct
     7681 tccgggtcgc gtgtagggtt gcagcggatg acgaccaatc gtgcgtgcgg tggatgacga
     7741 ctggtctaag agaaacgaga ccgccacgtc gcgtcgtcga cgcaagcgtt ttgtgtagat
     7801 ccgttcggac tggtagccat cagcgcggcg atcaattaca aaatcgatca ccgcattcag
     7861 atcaaactct tcgccatcga cttcattcgc cacgcgcgtg aggttctcag gcttcatcag
     7921 ttgaaactgg tgacggatcg acgagatgac gcccttgtgt cgttccttgg ttagatcgac
     7981 gaagttgcga tcgccatgct tcactttctt ctcgatcacg cgacaccagc cgaggcgatg
     8041 atcggtcaac tcacgatccc actcgtcgta gttgtacgcc acttcaccct cagcaagcac
     8101 ttgctcgggc gtctcagctt ctgaccacgc ttcagcgccg gtgagctcgt ccggatcact
     8161 ctccgctgaa ggatcgttcc acgcgttgaa caactcgcgc gcatcgcgac gctccggcgt
     8221 acgctcggct tgtctctgct tcattcgatc gctcgcggac gaatcgtcgt cagactcggt
     8281 ttgatcttcc ggtgcttcca cctgctgtac cgaatcatcg accggcatta cggtttgaaa
     8341 cagatcgtaa acacgactcg tcgccatcaa cgtgtcagca acggtcatgt gttcgcgcag
     8401 caaatactcg ctgacgactg tctccaactc ggaaacgatc tgcccgtaag cgaggcgcga
     8461 atcttcacgt gcgccgccaa gcatcgtgat ctggaaaagc aattcaaacg gaacgagcgc
     8521 gccgggcagt cccgtgatgc ttggccggct ctggctgagg tgctgccgga tcagatcgag
     8581 gtcgcgactc aaaccacgat atgcacgccg cagcctgcga tcgatgcgac cgttttcgag
     8641 cgttccgaag acacggcgcg cgagctgcgg gaggggaaac gctttcagca cgtcgcggta
     8701 accaatgtct tctgtgtgtt cttgcgcctt ttcgtggcta ccttcttcat cggcgcgtag
     8761 ctgaacgatc tcatccgcga tcgcaaacgc atccagcgca tcgacgttct ctttcgaata
     8821 ctttgccgcg atcgccgagt gggccacgcg caacatccca ccgcgctcgt aagtcccaaa
     8881 ctcgatctgc cccgcagcgt gcgcggcgag aactttgtac aaacggaaat caagctcctc
     8941 gtcgccaaac tcgttcacga gcgaaggcag atggatcgtg cggccatcgt caatgcgtgc
     9001 ttcgtcaggc acagcggcaa gtggcgcgat ctcgacttcg cgtccggtga gcgcttcgac
     9061 gtagaggcga agcagatgtt ggatcgactc gagcgcaagg cctgcgctgc cactcagcag
     9121 tgcttcgtaa ctaccgcgtg tttcgagtga gaaataactg cggcgcgcgc gtgtgtcggt
     9181 gcgcccggaa gtaagcccgc gacgcgccca cgcttcaaac tgctcgatcg acacactgcg
     9241 taacgcgcgt gcgctcgatc ggaaacaggc gagagccgct tcgccgtcaa ccgcggcaat
     9301 ctctttcgtc aacgcgagca cacggtgcga gagcgcgatc gcggcggcgc gattcggatc
     9361 ggcgttgagt atgcgaatga atccgggact actgcgaata aacgcgacca gactcgcgtt
     9421 gccgtgcatt gcgactaccc acagcagcgc gatccactca tcgaagatct ccggcgcgcg
     9481 gcggagaatc tcaccgcctg cgatcagaac gtgaagagcc gcaccaccac cgcgctcgag
     9541 aaagctgctt gcggttttca ataatcttat cgcgtccgcc gcggagaggt cggcgatggc
     9601 ttccggcgtg atggcccaag catcggcggc aatgccacct gcgcgtgccg cgaatgcgcc
     9661 gctaagcccg agtgcagccg cggttacagc cgggtcttga aacgacttca gcgcgcgcga
     9721 aaccgtactg ccggcattga gaaactcggc gctgtgtttc gccgatcggc gtgcgatctc
     9781 tgtcgcgacg tcgagtatgg cgcgcagcag ctcgtcgtca tcgatctctg cggccagtcc
     9841 aggaaaggag tgaagagttt cgcgaccgat agcagtcgag aggaccatct gtcgtgaaca
     9901 caaaacgaag atcgaagggc gcaactctgg tgtaactgtt cgcagtgctt cgacgccttc
     9961 agcgaagaac gttgctgctg attcgtagtc cgtcatcgcg agacgacggc ccatctctcc
    10021 ccacgcgcgc agctccgtgg gttgcaacac gtgtgcggcc gcgggcgcgg tgcgcaaaaa
    10081 ttcgatgccc gcgcggagcg agatagcagc aatcgacgcg ctcgtctcga gcactgccgc
    10141 cgcttggtcc aacggcaggc ctgttagact gcgggttagc tcaagaaaag actggcgctc
    10201 agcggaatgt tgatcttcgg ccacggattt tacggatttt taaaaacaga tcatcacgga
    10261 tcagattacg gtggttacga tttcgcggat gctgcgctgg agctcgcggt cgtcggtgat
    10321 gggagagcac atggcgacgt cggcagcatc gactggaggg atgccgagtg cgatgagttg
    10381 ggcagcgtag attagtagtc gcgttgaaac accttcttcc aaaccatgcc cgcgcaggtt
    10441 acgaaccttc tgtccgatca gcacgaggtc gcgtgcggtg ccctcatcaa cgccgccttc
    10501 gcgcgccacg atctccgctt ccatctcagg tggtggatag tcaaactcga ttgccacaaa
    10561 ccg
//
LOCUS       DASSFQ010000126         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_788700, whole genome shotgun sequence.
ACCESSION   DASSFQ010000126 DASSFQ010000000
VERSION     DASSFQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788700"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VFH96_04345"
     CDS             <1..884
                     /locus_tag="VFH96_04345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6973227.1"
                     /translation="RQPSGSFRKVKTPLLSVVIPTWNRAQLVGDAIRSVLAQGAVEVI
                     LVDDASTDSTVDLLESQFGDRIRLLRLEHRRGPGGARNAGARLARGEFVAFLDSDDVW
                     LPGKFAAELEVFARFPEAEVVVSDSQNFFEGIADGESRFAQNGLLETTRREVRLASDC
                     HWLWTNSMNTVHTCSITVRREALARLGEKLFAEDLVCCEDWEFQMRLYHACRVVVLPQ
                     VWSHVRRFVDPSRMGRNVPGRPTTPEQEMILLRSRLTVMERSDWLQGLDAKLTDELER
                     FRKKTATDFHGLVADYG"
     gene            877..1626
                     /locus_tag="VFH96_04350"
     CDS             877..1626
                     /locus_tag="VFH96_04350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6973228.1"
                     /translation="MVEEVHLWRGTQEQRALRSQLVKATQFAYFDAQLDRPEWSDKRV
                     LDFGGNKGNLLLDPACTISHSNYYCLDIIRDAIEEGRKTFPQANWFHYDRYNCSFNPE
                     GVVDLPVPDLGVEFDFILAYSVFTHTGWEETKELVEQLRARLAPGGVLAFTFIDPHWE
                     SNLRWRLEKRNTADRVAALLAKSREADWCSLVNGTELYVNSAGSWDEATERCITYNVY
                     FAADFLRAQFPGAQIKKPVNGEMQHCCELSR"
     gene            1610..2497
                     /locus_tag="VFH96_04355"
     CDS             1610..2497
                     /locus_tag="VFH96_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET6973229.1"
                     /translation="MSFPDNAKLNVDPAVARAILAAAERLPEYDNREFYSTDLQARVL
                     KSVRESCPEAFDEVINAIKERLERWPYCALVQGLSFDEDNRVFVAINRAFGELVARPY
                     EKPRAQLVHYIQPSTDIVSTRGGHESERLHTDCADWEPPVELISMVCVRADPEGGGRS
                     RVLDIDTIRNEVETHFGPDTLTRLETEPVPWQLAPYWGGGVSWRPVLTKSNMCWRRYT
                     IDMALDSTGATLSPGMLDALKHFEDLVSTTSHTLDFLMREGELLFSDNRRTIHARTPI
                     AAGPNSNRLMIRSWIRTSS"
     gene            2508..2933
                     /locus_tag="VFH96_04360"
     CDS             2508..2933
                     /locus_tag="VFH96_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973230.1"
                     /translation="MSKPKYAQLSDLIELDPIKHIDPKPLLGHWINSNPDTTSIARIN
                     ITEANGKLQLQVFGVGPDGPIDWGIAPAEVFAPNPSSSTGAGFTCTYDFGFAEARFQA
                     MIMKGLLVLAQFHTFKDDSKRASYFMREYYAMTHEHFNQ"
     gene            2930..>3140
                     /locus_tag="VFH96_04365"
     CDS             2930..>3140
                     /locus_tag="VFH96_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973231.1"
                     /translation="MNFKQKSHLHPDLLNPAAANLNPDKLLGRWINTNWETRGIAECT
                     VARDGEDITVNIIGVGEDGPIHWPTA"
BASE COUNT          691 a          875 c          894 g          680 t
ORIGIN      
        1 gccggcaacc atcaggttct tttcgcaaag tgaaaactcc actgctcagc gttgtgattc
       61 ctacgtggaa ccgtgcccaa ttggtgggtg acgcgatccg tagtgtgctc gcgcaaggcg
      121 cagtcgaagt tatcctcgtc gatgatgctt caacggattc gactgtcgat ctgctcgaaa
      181 gccaatttgg cgatcgcata cgtttgttgc gactcgagca ccgtcgcgga ccgggtgggg
      241 cgcggaatgc gggtgcgcgc ctcgcgcgtg gtgagtttgt cgcgttcctc gactcggatg
      301 acgtttggct gccggggaaa ttcgctgccg agctcgaagt gtttgcgcgt tttccggaag
      361 ctgaggtggt ggtttccgat agtcagaact ttttcgaagg cattgcggat ggtgagagtc
      421 gttttgcgca aaacggtttg ctcgagacga cgcggcgcga ggtacggctt gcgagtgact
      481 gtcactggct ttggacgaat agcatgaaca cggttcacac ctgctcgatc actgtgcgtc
      541 gtgaagcgtt ggcgaggctt ggtgagaagt tgttcgcaga ggatctggtg tgttgtgagg
      601 attgggagtt tcagatgcgg ctctaccatg cgtgtcgggt ggtcgttttg ccgcaggtct
      661 ggtcgcatgt gcgccggttt gttgatccat cgcgaatggg tcgcaacgtg cctggcagac
      721 cgacaacacc cgagcaggag atgatactgt tgcgatcacg gttgacggtg atggagcgat
      781 cggattggtt gcagggactt gatgcgaaac tgaccgacga gctcgaacgg tttcgtaaaa
      841 aaaccgccac agattttcac ggattggtag ccgattatgg ttgaggaagt acatctctgg
      901 cgaggaactc aggaacagcg ggcgctgcgg tcgcagctcg tgaaggcgac ccagtttgcc
      961 tattttgacg cacaactcga tcgtcctgag tggtcagata aacgggtgtt agatttcggc
     1021 gggaacaagg gaaatctgct gttggatccg gcgtgcacga ttagtcactc gaattattac
     1081 tgtctcgaca taattcgtga cgcaattgaa gaaggtcgta aaacatttcc gcaagcgaat
     1141 tggtttcact acgatcgcta caactgttcg ttcaatcccg agggcgtggt cgatttgccg
     1201 gtaccggatt tgggcgttga gtttgatttc atactcgctt attcggtttt tacgcataca
     1261 gggtgggaag agacgaagga actcgtcgaa cagttgcgcg cgcgccttgc accgggcgga
     1321 gtcctggcct tcacgttcat cgatccgcac tgggagtcga atctccgctg gcgcctggaa
     1381 aagcgaaaca cggcggatcg ggtcgctgcg ttgcttgcga agtctcgtga ggctgactgg
     1441 tgctcgctcg taaatggcac tgagctgtac gtgaacagcg ctgggtcatg ggatgaagcg
     1501 acggagcgtt gcatcacgta taacgtctac ttcgccgcag atttcttgcg cgcacaattc
     1561 ccgggcgcgc agatcaagaa accggtaaat ggagaaatgc agcactgctg tgagctttcc
     1621 cgataatgca aaactgaacg tcgatccggc tgtggcgcga gcgatactcg cggccgctga
     1681 gcgtctgccg gagtacgaca accgtgagtt ttactcgacc gatctgcaag ctcgcgtctt
     1741 gaagtctgtt cgtgaaagct gccctgaagc cttcgacgaa gttatcaatg cgatcaaaga
     1801 acgccttgag cgctggccct actgcgccct cgtacaaggt ctctcgttcg acgaagacaa
     1861 ccgtgtgttt gtcgccatca accgcgcgtt cggcgagctc gtcgctcgtc cttacgagaa
     1921 accgcgcgcg cagctcgtgc attacattca accctcaacc gacatcgttt ccactcgcgg
     1981 cggccacgag agcgaacgac tgcacaccga ctgcgctgac tgggagccgc cggtcgagct
     2041 aatctcgatg gtgtgcgttc gcgcagatcc cgaaggcggc ggccgctcgc gcgtgctcga
     2101 catcgacacg atcagaaacg aagttgaaac tcacttcggc cccgacacac tcacgcgtct
     2161 cgaaaccgag cccgtcccgt ggcaactcgc gccatactgg ggcggcggtg tgagctggcg
     2221 acccgtgttg acgaagtcga acatgtgctg gcgccgttac acgatcgaca tggcgctcga
     2281 ctcaaccggc gcgacgcttt cacccggcat gctcgacgcg ctcaagcact tcgaagatct
     2341 cgtttccaca acatcgcaca cgctcgactt cctgatgcgc gaaggcgagc ttttgttctc
     2401 cgataaccgc cgcacgatcc acgcacgcac gccgatcgcc gccggcccaa actccaatcg
     2461 cctgatgatc cgcagttgga taagaacatc cagttaatcc tgtaatcatg tcaaaaccga
     2521 aatacgccca actcagcgat ctaatagaac ttgaccccat caagcacatc gatcccaaac
     2581 cactgctcgg ccactggatc aactccaacc ctgacacgac cagcatcgca cgcatcaaca
     2641 tcaccgaagc aaacggcaag ctccagttac aagtattcgg cgttggcccg gatggaccaa
     2701 tcgattgggg catagcgccc gccgaggtct tcgcccccaa cccgtcatcg agcacgggcg
     2761 ccggcttcac ctgtacctac gacttcggat tcgcagaagc gcgtttccag gcaatgatca
     2821 tgaaaggcct cctcgtgttg gcccagtttc atactttcaa agacgacagc aaacgcgcga
     2881 gctacttcat gcgtgagtat tacgcgatga cgcacgaaca cttcaaccaa tgaactttaa
     2941 acaaaagagc catctgcatc ctgacttact caatccggcg gcggcgaatc tcaaccccga
     3001 caaacttctc ggccgctgga taaatacaaa ctgggaaaca cgcggtatcg ctgaatgcac
     3061 ggttgcgcga gacggcgaag acatcactgt aaacatcatc ggtgtcggcg aagatggacc
     3121 gattcactgg cccactgcac
//
LOCUS       DASSFQ010000127         9733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1990327, whole genome shotgun sequence.
ACCESSION   DASSFQ010000127 DASSFQ010000000
VERSION     DASSFQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990327"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFH96_04370"
     CDS             complement(<1..862)
                     /locus_tag="VFH96_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET6973232.1"
                     /translation="MLLVGLLETATGSLIVPIFDQAFVQGVGQRTPTIFGLQRLIPEA
                     PLGAWKMISILLIVFTLAKGIAEYFSTYLMARIGQSAVLKLRQDLYSHLLAQSATFFE
                     RHRTNYLVSRLVSSAAAIETAVTSTLRDMLRESFTLIFFLGASFYYSWRLALGSLLIA
                     PVIAVLTAKFGRALRNLARESFEGSKQLTDTAQEALANQGIVKAYRAEKRESERFTHV
                     AKKIVRANLRSASIAGASPPTIEMIGILFVVLLLFFGQREIIAGHMNTAQFVTFLFFL
                     FRSYDPMRKLS"
     gene            1142..1903
                     /locus_tag="VFH96_04375"
     CDS             1142..1903
                     /locus_tag="VFH96_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6973233.1"
                     /translation="MIKIPSDRIRHLAQQATPNTKHAGKRLLIADDDTDMRLLLAEYF
                     RRLGFQVEERESGMAALEATIGGRFDCFIFDVSMPGMSGIELLKRVRDRGIQTPALFL
                     TAHDALSDKVAGFEAGADDYLAKPFSPRELEYRVEALLRRGGAAPQESDGERIEVGDL
                     VIDKRRHEVIRNGFRVDLTPLEFQILELLASEPGRAWSRNALLDRVWSTDYEGYQRNI
                     DPHINRLRKKLETDPKNPRYVLTVRGVGYKLNEIP"
     gene            2187..3743
                     /locus_tag="VFH96_04380"
     CDS             2187..3743
                     /locus_tag="VFH96_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6973234.1"
                     /translation="MNFSRVVCAFSACLLVVSIAIAQQDVERKTVAITYPLDETVTVK
                     FRGTTLLPRLKGEAKVKRAGRRGTRVELSVEDLPRASELGGIYTTYILWAISPDGHVD
                     NLGEIKRGGSVFVNSKIDVTTPLQTFALILTAEPHFLMKVPSRMVVMENLPPQKPNGS
                     QVETVDVRYIGNSSDYFRTARVPEIADADYRQTPVSLLGARQAISLAKYAGASGDAPN
                     ELQTAEDHLQAAEKAWRMNQPLAEVDLEARKATSAGQHAEEVAAARKAARLNREEIRR
                     RDEAVRTAEESAEESQREVGKLRAALDKETRARELAERDAGTANDQLREKRVEIAQLR
                     DEIATLRADSDAAKLKLARLEGEKQAETARVEAEKRAVEKRAAEANLKETLAKYGSLK
                     ETATGFRLVLPETIWTSTRAATLSAASSAKLEPLAALLASNPDYQILIEAYTDSKGNE
                     VALQQLTQDRARVLSDRFQTAGVDPMRIQANGMGASNPLAPNTTVAGQRKNRRVEITF
                     TSPVGRTAAN"
     gene            3800..5824
                     /locus_tag="VFH96_04385"
     CDS             3800..5824
                     /locus_tag="VFH96_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973235.1"
                     /translation="MSPLDPLEILQVIGYSIGALLPLWMLFQLITHRSKLSNIERLLA
                     LLALTMGGWHTSNLVITLHGLFDLGPDTWTTLLRVADTVAVVSITFAYSFLLHVHLYL
                     WANAHGRSLNRSEQLRVYLSYIPTVFLIIAMPALWTGDYEPMFTKLSFFVIPFAFWIA
                     YVLGLIALTELLIARKSKNRSERRIMQTLAASFLGVGVVIFAALAAGVGEGTTAGLYL
                     KTIANLGSLLPSVLLAYYIYRYRYLEIIIEESLVVVTFATVVLTVYVFGIRTIGDWMT
                     QRYGTRPGLVEALLILALALLAAPLRGFLAQRFQKLFEREAALYKEIVARIGSYAGQY
                     KDLSDLLHFVEERTTEALGLRQLKIFVEDSEAWVHEVLEKSKESGWLPVEDEAVLNDQ
                     SFKIAYPLRREDKVQGLLAVDAASASLTPDARTILALLADQVAIAIDDSRLLGENLRL
                     ERELAERERLAALGRMAATVAHEIKNPLSAIKSIAQVMREDENLRNEYERDLGLIVGE
                     TDRLSQSVTQLLSFARKESPAVQPLSVDELVRSVVDLFRASAREQGIVLDLQVKVEAE
                     LAGKSVSALRDALSNLLLNALQATPAGGRVELIAAQSDGELVISVQDSGTGVPVDLRE
                     RIWEPFFTTRQRGTGLGLAIVRKRVQEVGGTATLVSGNGSGALFQLRLRM"
     gene            5991..6614
                     /locus_tag="VFH96_04390"
     CDS             5991..6614
                     /locus_tag="VFH96_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973236.1"
                     /translation="MKIKNFLTAMLFVLCAAVCLNVNAQSAPDPNDDANLDTQLYLIV
                     GTNQDVDDTKLPASLDAVVKQLRSTLPFKNYRLAATLINRVKNEGRLDLSWIGGTLAS
                     PTGAASNVTPSFSQFRVRQVKLVRNAENQQIVQMLGFNFGARVPIQTPGAIAANGAVA
                     PTVAYEQTGVSTDISMRESEPVIVGTLNIGPSGDAIILVVSAKRTPR"
     gene            6673..8130
                     /locus_tag="VFH96_04395"
     CDS             6673..8130
                     /locus_tag="VFH96_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6973237.1"
                     /translation="MRKDALRLMIVDDEEAARYGMRRALSTFGYNITEAGSAEAARAL
                     LKQNEPDLLLLDVNLPGLSGLDFLRELKSANGNAPLVIIVTAHGSERMAVEAVKAGAH
                     DYISKPFELDDLRLVIKNAAETVQLRRENYSLRRRIEVERSQRGALIGNSDAMQKVRA
                     MIDKVAETDATVLVRGESGTGKELVARELHERNSVRRNASFVAVNCAALPSELIESEL
                     FGHEKGAFTGAAARREGKFEQADSGTLFLDEIGDMSSNVQAKLLRALEERRIERLGAN
                     DSIPVDVRIVSATHRPLEQEITNGNFRADLFYRLRVVTVEIAPLRERREDIPLLIETF
                     TRLAAERYELPQRSLSQGALRRLMEYNWPGNVRELKNTIDRAVIMADGDEIAPKDLPD
                     EITAGISKDGIVENSDAEIDGLRVPFTADFREDRREFERRYISRCLEYTQGNVTRAAE
                     ILDMHRQSLQHKLRQLGLGRRYVSVTGDVQDNPDS"
     gene            8140..8781
                     /locus_tag="VFH96_04400"
     CDS             8140..8781
                     /locus_tag="VFH96_04400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET6973238.1"
                     /translation="MKAKFDAIIQPEQAKYLDQLLTQSDPLLAEMEAYAAEHRVPIAD
                     REVARFVEITARISGARKALEIGMAIGYTVVHLARGMGENGRVVTIDPSDEMITAATG
                     YLKRAGVLNQVEIERGKALEVMPNLEETFDLLFIDALKEEYSQYLDLGLQRLRSGGVV
                     IVDNLLWGGKVATGDTESSTVALREFNPYFMNHPELIAEVLPVGDGLGYAVKR"
     gene            8940..>9733
                     /locus_tag="VFH96_04405"
     CDS             8940..>9733
                     /locus_tag="VFH96_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6973239.1"
                     /translation="MSSGKFRQIVGLVLLASLAFSTYSCTVVSSNEEFFGTTTPPARD
                     IFRYVTGDEPATLDPALGNGQPEARIYMALFEGLVEYNPKTLEPEPALAERWHINNDS
                     SEFTFHLRTNGRWSNGDPIDANDFVYSFRRALDKKTASRNAALASYLKYAQAWNEQRV
                     FVRDPQTGQFLLERDFQENPPDESIGSSPIDKAKSEYVPDPNESTPDPDSAFHRQMHS
                     PLRLTLPGNEKARTTQIEANPKLKAAMAGKEFVPVKGEDLGVEALD"
BASE COUNT         2259 a         2676 c         2749 g         2049 t
ORIGIN      
        1 gcgagagctt ccgcatcggg tcgtaactgc gaaacaggaa gaaaaggaac gtgacgaact
       61 gcgccgtgtt catgtggccc gcgatgatct cgcgctggcc gaagaacagc agcagcacga
      121 caaacagaat tccgatcatc tcgatcgtcg gcggtgaagc gccggcgata ctcgcactgc
      181 gcagattagc tcgcacaatc tttttcgcca catgagtaaa gcgttccgac tcgcgcttct
      241 ccgcgcgata cgctttcacg attccctggt tggcaagcgc ttcctgggcg gtgtcagtca
      301 gttgtttgct gccttcgaaa ctctcgcgcg caaggttacg caacgctcgg ccaaacttcg
      361 ccgtcaacac cgcgatcacc ggggcaatca ggagagatcc gagggcgagt cgccaactgt
      421 aataaaagga cgcgccgaga aaaaagatta gcgtgaaact ctcccgcagc atgtcgcgga
      481 gcgtgctcgt gactgccgtc tcgatcgcgg ccgcgctgga gacgagtcgt gagacgaggt
      541 agttcgtgcg atgtcgttca aagaaagtcg ccgactgcgc gagcaggtgg ctatatagat
      601 cctggcgcaa cttgagcacg gctgactggc caatgcgcgc catcaggtaa gtagagaagt
      661 attccgcgat gcccttggcg agcgtgaaaa cgatcaacag gatcgagatc attttccagg
      721 cgcccagcgg agcctcggga attaaccgtt gcaggccgaa aatcgtcggg gtacgctggc
      781 caacgccttg aacaaatgcc tgatcgaaga tgggaacaat caaactgccg gtggcggtct
      841 cgagtaaacc gacgagaagc attgcgagag tggccagcgt gaagattggc cagtgaggtc
      901 tcaaatactt taacaggcgg cgtagatcac gcatctctgg ttgcgaaaat ctctgatcag
      961 ttgctataag ggaggtagaa aaatcgccca aaatccgtaa gaaagaacta tacttacccc
     1021 cacttatatg cgtcaaggct gactgatttg ctcgatttgt ttctgggcgt gaatcatgca
     1081 aatcttttcc ccgcatgagt ctgcctcaat ttaggttata attcgtccgc taaaaacgcg
     1141 ggtgattaag atcccctccg acaggatcag acatttggca cagcaagcga caccaaacac
     1201 caagcacgca ggtaaacgtc ttctcatcgc agatgacgat accgatatga gacttctcct
     1261 ggccgagtat ttccgccggc tgggctttca agtggaagaa cgcgaaagcg gcatggcggc
     1321 actcgaagcc accatcgggg gacgattcga ttgtttcatc ttcgacgtat cgatgcccgg
     1381 catgtcaggc atcgagctgc tcaaacgagt tcgcgatcgc ggcattcaaa cgccggcgct
     1441 attcctgact gcacatgacg cgctttcaga caaagttgca ggcttcgagg caggcgcaga
     1501 cgactatctc gcaaaaccgt tcagtccgcg cgagctggag tatagagtcg aagcactgtt
     1561 gcgacgcgga ggcgcggcgc cacaggaatc ggacggtgaa cgcatcgaag ttggcgatct
     1621 cgtgatcgat aaacgtcggc acgaagtgat acgaaacggc tttcgcgtcg atctgacgcc
     1681 gctcgagttt cagattctcg agctgctcgc gtctgaaccg ggacgagcat ggtccagaaa
     1741 tgcgctgctc gatcgagttt ggagtacgga ttacgaaggc taccagcgca acatcgatcc
     1801 gcacatcaat cgacttcgta agaagctcga gaccgatccg aaaaatccgc gatatgtcct
     1861 gaccgttcgt ggcgtcggtt acaaactgaa cgagattccc tgatgctttg gtgggtcgga
     1921 tcagggctga tacttgcctg gctaatcctc cggctagtca aaccgatggg ctggattccg
     1981 atgctgctga tctcgggagt ctctatcctt attatccagc tcgccgccta ccgcaaggcc
     2041 aaatccgtta aataatccgt gtcatccgtg gctaattcgt cttgacctcg cctactcgtt
     2101 cggcaacaat gcgcgtgttt atggcatcaa ggacattgtt aggtctagcc aactttccca
     2161 gatctaagta ccaggagttg ttagccatga attttagccg cgtcgtttgt gcattcagcg
     2221 cttgtctact cgttgtttcc atcgccattg ctcaacagga cgtcgaacgt aagaccgtag
     2281 ctataactta tccgctggac gaaacggtta cagtcaagtt tcgtgggacg actctgttgc
     2341 cgcgcctgaa aggtgaggcg aaggtgaaac gcgccgggcg ccgcggcacc cgcgtcgaat
     2401 tgagcgtcga ggatctgcca cgcgcctccg agctgggcgg catttatacc acttacattc
     2461 tctgggccat ctcgcctgac gggcacgtcg ataacctcgg cgagatcaaa cgcggcggca
     2521 gtgtctttgt taattccaag atcgacgtca ccacaccgct gcaaacgttc gcactcatcc
     2581 taaccgccga gccgcacttc ctgatgaaag tgccgagccg catggtggtg atggagaatc
     2641 taccgccgca gaaacccaac ggtagtcagg tcgagactgt tgatgtgcgc tacatcggta
     2701 actcgagtga ctacttccgc acggcgcgtg ttccggaaat cgccgatgcc gactatcgcc
     2761 agactcccgt ttcgctactc ggcgcacgac aggcaatcag tctcgcgaag tacgcgggcg
     2821 catcggggga tgcaccgaat gagttgcaga ctgctgaaga tcatttgcag gcggcagaga
     2881 aagcgtggcg gatgaatcag ccgctggctg aggttgatct cgaggcgcgc aaagcaactt
     2941 cggcgggtca acatgctgaa gaggtcgccg ccgcgcgtaa ggctgcacga cttaatcgtg
     3001 aggagattcg tcgtcgtgat gaggcggtgc gaactgcgga agaaagtgct gaagaatcgc
     3061 agcgtgaagt agggaagctt cgtgcagcac tcgacaaaga aacacgcgcg cgtgagctcg
     3121 ctgagcgcga tgcaggtact gcgaacgatc agctccgcga gaagcgagtg gagatcgcgc
     3181 aactgcgtga tgagatcgcg accttgcgcg cggacagtga cgccgcgaag ttgaaactcg
     3241 cgcgactcga gggtgagaag caggccgaga ctgcacgcgt cgaagcggaa aaaagagcgg
     3301 tggaaaaacg agctgcggaa gcgaatctga aagagacact cgcgaagtac ggctcgttga
     3361 aagagactgc caccggtttc cgattagtac tgccggaaac aatctggacc agcacgcgcg
     3421 ctgcgacatt gtctgctgcg tcatcggcga agcttgagcc gctggcagcc ttgctcgcca
     3481 gcaatcctga ttatcaaatc ctgatcgagg cttacactga tagcaaaggc aacgaagtgg
     3541 cgctccagca gttgacgcag gaccgtgcgc gggtgttgtc agatcggttt cagacggcgg
     3601 gagtagatcc gatgcgcatt caggcgaacg gcatgggcgc ctcaaatcca ctcgcaccaa
     3661 acacgacggt cgcaggtcag cggaagaacc gccgcgtcga gatcacgttc acctcgccgg
     3721 tgggacggac cgctgcgaat tagctcagtg atcaatatag gtcctattcg tcctatagga
     3781 cctatattga tcaattgtca tgtccccact tgatcccctc gagatactcc aggtcatcgg
     3841 ttacagcatc ggcgcgctgc tgccgctctg gatgctgttt cagctcatca cgcatcgcag
     3901 caagctgagc aacatcgagc gcctcctcgc cttactcgcg ctcacgatgg gcggctggca
     3961 caccagcaat ctcgtcatca cgctgcacgg tctcttcgat ctcggccccg acacctggac
     4021 cacgttgctg cgcgtcgccg ataccgtcgc cgtcgtcagc atcactttcg cttattcgtt
     4081 cctgctgcac gttcaccttt atctctgggc caatgcacat ggtcgctcgc tcaatcgcag
     4141 cgaacaacta cgcgtttatc tctcttacat ccccaccgtc tttctgatca tcgccatgcc
     4201 tgccctctgg acgggcgatt acgaaccgat gttcacgaaa ctctcgttct tcgtgattcc
     4261 tttcgcgttc tggattgcgt acgtgctggg actgattgcc ctgaccgagc tactgatcgc
     4321 gcgcaagagt aagaaccgga gcgagcgacg catcatgcaa actctcgcgg cgtcattcct
     4381 cggtgtcggc gtcgtgatct ttgctgcgct tgcggccggc gtcggcgaag gcacaacggc
     4441 cggactatat ctaaagacga tcgcgaacct cggctcgctc ctgccctcag ttcttttggc
     4501 ttactacatc tatcgctatc gctatctcga gatcatcatc gaagagagtc tcgtcgtcgt
     4561 tacttttgcg acagtggtgt tgacggttta tgtcttcggt attcgcacga ttggcgattg
     4621 gatgacgcag cgatacggaa cgaggcccgg actcgtcgag gcgttgctga ttcttgccct
     4681 cgctttattg gccgctccat tgcgcggttt cctggcccaa cgatttcaaa aactcttcga
     4741 acgtgaagca gcgctctaca aagaaatcgt cgcgcgcatc ggctcgtatg cgggccagta
     4801 caaagatctt tcggatctcc tgcacttcgt agaagagcgc acgactgaag cgctcggcct
     4861 gcggcaactc aagatctttg ttgaagattc tgaagcgtgg gtccatgagg tacttgaaaa
     4921 atcgaaagag agtggctggc tgccggttga agatgaagca gtgttgaatg accagagctt
     4981 caagattgct tacccgttgc gtcgtgaaga caaggtgcag ggcttgttgg ctgtcgatgc
     5041 agcgtcagcg agtttgacgc cggacgcgcg tacgatactg gccttactgg ccgaccaggt
     5101 tgcgatcgcg atcgatgact cacgactgct tggagaaaac ttgagactcg aacgcgagct
     5161 tgccgaacgc gagcgtctcg cggcgctggg tcgcatggca gcaacagtgg cccacgagat
     5221 caagaatcct ctgtcagcga tcaagtcgat cgcgcaggtg atgcgtgaag atgagaactt
     5281 gcgcaatgag tacgaacgcg atctcgggtt gattgttggt gagactgatc gactgagcca
     5341 gagcgtgacg cagttgttga gttttgcgag gaaggaaagt ccggcggtcc agccgttgag
     5401 cgttgatgag ctcgtgcgat ctgttgtcga tctttttcgt gcgagtgcca gagagcaggg
     5461 catcgttctc gatctgcaag tgaaagtcga ggcagagctg gcgggcaaat cggtttcagc
     5521 gttgcgagat gctttgtcga atctgttgct caatgcacta caggccacac cagccggtgg
     5581 aagagtcgag ctgatagcag ctcagtcgga tggtgagctc gtgatcagcg tgcaggacag
     5641 cggcacgggc gtgccggtcg atttgcgaga gcgaatctgg gagccgttct tcacgacgcg
     5701 tcagcgcggg accggattgg gactcgcaat cgtgcgcaaa cgtgtgcagg aagttggcgg
     5761 aacggcgacg ctcgtgagtg ggaatggtag tggggcgcta tttcaattac gcctgcggat
     5821 gtagagacag ttgcagacaa gatttacaag atatttcatg attgacatga ttcaaatctt
     5881 gtctatcttg aaacatcttg taaatcctgt ctctaaacgg aactttacat ccatgcctgc
     5941 gtgttagggt ttgacctgac gacctatcga ttgaaggaga acgacccgca atgaagatca
     6001 agaactttct caccgcgatg ctgttcgtac tgtgcgctgc agtatgtttg aatgttaacg
     6061 ctcaatccgc gccagatccc aatgacgacg ctaatctcga cacccaactc tatctcatcg
     6121 tcggcacaaa ccaggacgtc gacgacacaa aactgcctgc gtcactggat gcggtagtga
     6181 agcaattgcg ctccacattg ccattcaaga actaccggct cgctgccaca ctgatcaatc
     6241 gcgtgaagaa tgaaggccgt ttggatctga gctggatcgg tggaacactg gcatcaccaa
     6301 ccggtgccgc atcaaacgta acgccaagct tcagtcaatt cagagtgcgg caagtgaaac
     6361 tcgtacgcaa cgctgaaaac cagcagatcg tgcagatgct cggcttcaac ttcggcgctc
     6421 gcgttcctat tcaaacaccc ggcgcgatcg ctgcgaacgg cgcggtagcc ccaaccgtcg
     6481 cctacgagca aaccggagtc tcgaccgaca tcagcatgcg cgaatccgag ccggtgattg
     6541 tcggcacact caacattggt ccatcaggcg acgccatcat cctcgtcgtc tccgcgaaga
     6601 gaacaccacg gtaatctgtg gcacgcaacg cgctaggagt ttagggccac acggccctaa
     6661 actactcagg aagtgagaaa agacgcgttg cgattgatga ttgttgacga cgaggaagcc
     6721 gcccgctatg gcatgcggcg cgccctctcc acctttggct ataacatcac tgaagcaggt
     6781 tccgccgagg ccgcccgtgc cttactcaaa caaaacgagc cggacctgct cctcctcgac
     6841 gtaaatcttc ccggactcag cggactcgat tttctccgcg agctcaagag cgcaaacggc
     6901 aatgcaccgc tcgtgatcat cgtcaccgcg cacggctccg aacgcatggc cgtcgaagcg
     6961 gtcaaagccg gcgcccacga ttacatctcc aagcccttcg aactcgacga tctgcgactc
     7021 gtaatcaaaa acgccgccga gacagttcaa ctacgtcgcg agaattattc actgcgacgc
     7081 cggatcgaag tcgagcggtc acagcgtggc gctctcatcg gcaactccga cgccatgcag
     7141 aaagtgcgcg cgatgatcga taaggttgcg gagactgatg cgaccgtgct tgtgcggggt
     7201 gagtcaggaa cgggcaagga gctcgtcgcg cgcgagctgc acgagcgtaa tagcgtgcgc
     7261 cgaaatgcct catttgtcgc ggttaactgc gccgcattgc cgtccgagtt aattgagtca
     7321 gagcttttcg ggcatgaaaa aggcgcattt acaggcgccg cagcacgccg ggaagggaag
     7381 tttgaacagg ctgacagcgg cacgttgttc ctcgacgaga tcggcgatat gagctcaaac
     7441 gtgcaggcaa agctactgcg cgcgttggaa gagcgacgca tcgagcgtct cggtgcaaac
     7501 gattcgattc ctgttgacgt tagaatcgtg agcgccactc atcgcccgct cgaacaggag
     7561 atcactaacg gaaatttccg cgcggatctc ttctatcgtt tgcgggttgt gacggttgaa
     7621 atcgcaccgc tacgcgagcg tcgcgaagac attccactcc tgatcgagac cttcacgcgc
     7681 ctcgctgccg agcgttatga gctgccgcag cgttcgttga gccagggtgc attacgacgt
     7741 ctgatggagt acaactggcc gggcaatgtg cgcgagctaa aaaacacgat cgatcgcgcc
     7801 gtgatcatgg ccgacggcga cgagatcgcg ccgaaagatc tgccggacga gataaccgca
     7861 ggcatttcca aagacggcat tgtcgagaac agtgacgccg aaatcgacgg tttgcgggtg
     7921 ccgttcaccg ccgatttccg tgaagaccgg cgcgagtttg agcgtcgtta catcagtcgc
     7981 tgtctcgagt acactcaggg aaacgtgacg cgcgccgccg agatcctgga catgcaccgg
     8041 cagtcgctgc aacataaatt gagacaactc ggcctcggcc gccgctacgt ctcagtcacg
     8101 ggcgacgtgc aagataatcc tgacagttga cgtaaaaaca tgaaggctaa attcgacgca
     8161 atcattcaac cagaacaagc gaaatacctc gaccaactcc tgacccagag cgatccttta
     8221 ctcgccgaga tggaggctta cgcagccgaa catcgcgtac cgatcgctga ccgtgaggtg
     8281 gcacgtttcg tggaaatcac ggcgcgcatc tcaggcgcgc gcaaggccct cgagatcgga
     8341 atggcgatcg gatacacggt ggtccatctt gcgcgtggta tgggtgagaa tggacgggtg
     8401 gtgacgatcg atcccagcga cgagatgatt actgcggcca ccggctatct caaacgcgcg
     8461 ggtgtgctga atcaggttga gatcgagcgt ggcaaggcgc tggaagtgat gccgaatctc
     8521 gaagagactt tcgatctgtt attcatcgac gcgctgaagg aagaatactc acaatacctc
     8581 gatctcggtt tgcagcgcct gcgcagtggc ggcgtcgtaa ttgtcgacaa tctactgtgg
     8641 ggcggaaaag tcgcgaccgg cgacacggaa agttcgacag tagcgctgcg agagttcaat
     8701 ccatacttca tgaaccatcc ggagctgatc gccgaagtct tacctgttgg cgacggctta
     8761 ggctacgcag tcaaaagata gccgcaaaac gcacataatg taatttagcc cgtgtggtga
     8821 aattcattat gtgctttttg tgcctttttg tggcaaactt ccacgcgtct cttcttgcgg
     8881 gcttcctcat aactcgcact tgaacacaac gtgactgcat ttgagtcgga ggtgcaccga
     8941 tgagtagtgg caagtttaga cagattgttg gactcgtctt gctggcatcg ctagcgttct
     9001 caacttactc gtgcaccgtg gtgtcgagta atgaagagtt cttcggaaca acaacgcctc
     9061 ccgcacgcga catctttcgt tacgtcacgg gcgacgaacc cgccacgctc gatcctgccc
     9121 tgggtaacgg ccagcccgaa gcccgaattt acatggcgct gttcgaaggt ctcgttgaat
     9181 acaacccaaa gacgctcgag cccgaaccgg ctttagcaga gcgctggcac atcaataacg
     9241 actcatccga gtttactttt cacctgcgca ctaacggtcg ctggtccaac ggcgatccca
     9301 tcgacgcgaa cgactttgtc tacagtttca ggcgtgcact tgataagaag acggcttcac
     9361 gcaacgctgc tctcgcttca tacctgaaat acgcgcaggc ctggaacgag caacgcgttt
     9421 tcgtgcgcga tccacaaaca ggccagtttc ttttagagcg agattttcag gaaaacccgc
     9481 ccgacgaatc gattggcagc agtccgatcg ataaagcgaa gagcgaatat gttcccgatc
     9541 cgaatgagtc gactcccgat ccggatagtg cctttcatcg gcaaatgcat tcaccgttgc
     9601 gcttaacgct gccgggcaac gagaaggcgc gtactacgca aatcgaagca aatccaaaac
     9661 tcaaggcagc catggcgggc aaagagtttg tgccggtgaa gggcgaagat ctcggtgtcg
     9721 aagctttaga caa
//
LOCUS       DASSFQ010000128        10728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1385078, whole genome shotgun sequence.
ACCESSION   DASSFQ010000128 DASSFQ010000000
VERSION     DASSFQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385078"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VFH96_04410"
     CDS             <1..1273
                     /locus_tag="VFH96_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973240.1"
                     /translation="PFRKGTTSVRLGYGIYHEQVSISAAELLALNPPFQETATQTLTS
                     MDQPIAPGSAIPVVASATPASIRAIQPHFLTPYMQQWSLDVQHQIGNKTIFTLGYYGS
                     KGTHLNGNTEYNDLPVGFAINSQCATGTAALQDAGVVTTQCMTPGTALTATPTIFDQI
                     RPFRGYRSINVLETRYNSNYHSLQAYLQHRFSGASQFNFAYTWSKNLTDNQTSSVNAA
                     PQDVNNIRAEYSRAILDRRHIVSANWIYELPFFAKSNGLTHTVLGGWQVSGIGSYYTG
                     LPFTVASATYDTAGIGFIPAIVAGGRPRLLCDPNENAPHTVDQWFNGACFAKQTPAGA
                     TGILNVPGDASRGSVNGPPTTRVDFTLAKSFSFSESTSIQFRAEAFNVFNHTNFRNLS
                     VSRAITSEALFGSVTSFRDPRVLQFGLKFLF"
     gene            1337..2473
                     /gene="nagA"
                     /locus_tag="VFH96_04415"
     CDS             1337..2473
                     /gene="nagA"
                     /locus_tag="VFH96_04415"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET6973241.1"
                     /translation="MKTLLLKNANVVLADREVQASVLIENGRIVSLEAGEGDEEIDLA
                     GTTLLPGFIDVHIHGAAGVDVMDATAAGLREVSEYLASQGVTSWVPTFVPASDEHYAS
                     AIAVISAAMNGPGARVLGVHYEGPFVNTAQCGALHTEYFKSYSQPEELEFLVVPPAGV
                     RMITMAPEVAGGVELVRELKRRGWVISIGHTRASLEVLDDACEAGAHHMTHFMNAMAP
                     LHHRSPGPIAWGLSRDDVTVDVIADGIHLDPFMLRLLLKLKGANGISLISDAIAAAGK
                     GDGDYEIWGETISVKDGRTANAHGSIAGSVISMLDAVRLLHSLGVSYVDLARMASSNP
                     ARLLGVDNDCGSIEVGKRADLVALDNDNNAKLTLIGGKSVPNPH"
     gene            complement(2432..2821)
                     /locus_tag="VFH96_04420"
     CDS             complement(2432..2821)
                     /locus_tag="VFH96_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973242.1"
                     /translation="MENKTLNQKTLTIFAPVLILIGIAGFIIPERYSLTSGAAPYNLF
                     HIFFGALGLLLVMTNKDVLASLFNFGFGLIDLYQALASVVGLTPIEYFHWTYVDDVLH
                     VLIGFALVIIGGYGLMRIWNGFTADQS"
     gene            complement(2821..4086)
                     /locus_tag="VFH96_04425"
     CDS             complement(2821..4086)
                     /locus_tag="VFH96_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET6973243.1"
                     /translation="MSTNLARQEDKLLACIHCGLCLEACPTYVITGNENDGPRGRLYL
                     MRAVAEGRLEKTSSAFATHIDRCLGCRACEQVCPAGVEYGQLLEVSREVLLEAQPKSD
                     LANRLLRFVLRHVWLSPWRLRMFFAASRVFRDLRLARLLLKTGVLPRRAEFGMALLES
                     SRAVSPSAAQRRDGKHKAAAESVLLFKGCVGEGLFARVNRATERVLEANEFEVETPAK
                     QVCCGALHAHAGDLEGARELARRNIEAFDSDAPIITNAGGCGAMLKTYAHLFGNDERA
                     MKFSARVRDVSQQLATTEMRTAPSPTGQTVTYDTSCHLMYGQHSGEASERMLRSVTGK
                     DFVPLNGTERCCGAAGVYNLLQPEMSQRVLNEKLDHIKETGAGILATGNPGCQMHIGA
                     GACLSGMKLRICHPIEIVDQAYEAAGYYE"
     gene            complement(4151..5860)
                     /gene="ggt"
                     /locus_tag="VFH96_04430"
     CDS             complement(4151..5860)
                     /gene="ggt"
                     /locus_tag="VFH96_04430"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET6973244.1"
                     /translation="MKTQIKVIVLLLVVALNVPLTIAREPVRGRHGMVASTNEVASRV
                     GVEIMKRGGNAVDAAIAVGFALAVTHPAAGNLGGGGFMMIRLKDGRTTAIDYREMAPD
                     AAHRNVYLDKNGNLIEGEGGSLVGYRAAGVPGTVRGMELALRKYGSGKLTWAQLVEPA
                     RQLAGRGFVVTNELARSLYSNREYLEKYAETKRIYLKGGKFYQEGELFRQPELAATFA
                     RLQRFGPNEFYQGETARLIVADMKRNNGLMTMDDLRGYVAKERTPVRGNYRGYEIISM
                     PPPSSGGAVLIQMLNILEGFDLQKLEANSSDRYHLMAEAMRRAFADRAEYMGDSDFVK
                     VPVPGLIDKAYAATLRKTINTERASTSEEIRAGRPAGYESDETTHFTVVDAEGNAVSN
                     TYTLNNSYGSAAVAKGTGLLLNDEMDDFAAKPGTPNMYGLIQGERNAVAPKKRPLSAM
                     TPTIVLRKDGSLWFTVGSPGGPTIINTVLCVITNVIDYEMNIQQAIDFPRIHHQWLPD
                     ELVGEPFGLSGDTQKALAARGHKLAKLRYLGDAEGIMIEEKTGVRLGATDPRRSDGLA
                     IGY"
     gene            complement(5886..8282)
                     /locus_tag="VFH96_04435"
     CDS             complement(5886..8282)
                     /locus_tag="VFH96_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6973245.1"
                     /translation="MDTLINNLAAIVGRENVLSKPDELLVYECDGLPQHKHRPRAVVF
                     PASTDETSAIMRELARANVPFTPRGAGTGLSGGALALNSGVVIEMARMRKILRIDTEN
                     RIAVVQPGVVNLHVSRAVAPFGLYYVPDPSSQPSCTIGGNIAESAGGIHCLKYGTTTD
                     HVLGCRVVLAGGDVVDLEIDSPGYDLLGTFIGSEGTFGIATEAALRLAQSPPAVRTLL
                     AEFMEVNDASHAVSAIIAAGVMPAALEMMDHEIIRAVEASVFAAGLPPDAGAALLIEL
                     DGIEAGLDDEAHKVTSICMEYGARSCRHARDESERKKLWAARKGAFGAIGRISPDSMI
                     QDAVVPRSRLPQVLGAAYDIASRYQLRIANVFHAGDGNLHPLICFDSRFPDEVRRVKD
                     AGRELMEVCVAAGGTITGEHGVGLDKRELLPLVFSDADMNAMLSVRAAFDPLGLCNPG
                     KIVPMLRGCGEAKAVASDNGSAAQMQPVIAPVVEKPEVEVATPTEFNPDRAVTLLAQI
                     VGDVNIASLNGTIVVSPASAEEISDVLRLASSERWTVVPAGGMMGVSSRANLIVSTRR
                     LNRIIEHEPADLIAIAQSGVTLTDFNAKLAENGQWLPLDPPDDGRATLGGVVATGIGG
                     PQQFGYGRPRGSVIGMKVVLADGSTIKAGGRVVKNVAGYDLCKLFTGSYGSLGIITEL
                     NFKLRPRPAREATVIGTGSARAILDARLFPVALEIVDDVLHVRFAGNEKGVAFQIEQA
                     LKLLQNAEIVMDDAELWRRIAAARALSGRARKEPSGTAKLLMQRIKRQLDPKNILAAD
                     "
     gene            complement(8370..9659)
                     /locus_tag="VFH96_04440"
     CDS             complement(8370..9659)
                     /locus_tag="VFH96_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6973246.1"
                     /translation="MQNQPEADLVRGIRRWDLVAITINGIIGAGIFGLPSNVYKLIGT
                     YSLIAFVACALVVALIILCFAEVSSRFDETGGPYLYAREAFQPAVAFGVGWLIWLARM
                     TAFAANCNLLINYVSYFWLPATTPLWRASIIVLIVIALAAINLLGIRQAAIVSNAFTV
                     GKLVPIIIFIAAGLFFLNPQAFEFGPSPTTADFSKSVLLLVYAFTGFEMATIPAGEVR
                     DPQKSLPRALLIAILVVAILYIMIQVVCVGTLPGLAQSTKPLADAGSQFLGAAGGAII
                     SAGAIISITGNLNILLLSGSRLPFAMAEQKQLPAFVGSIHRKFFTPYVSILITAGLML
                     FLTLKSSFVAALTISTIARLVTYAATCLALPVFRARRNAPAAMFRLPGGTVVAILSLL
                     LIVWLLRNARFDEVKATAYAAGTGLLIYLAYWFYKRV"
     gene            9707..10639
                     /locus_tag="VFH96_04445"
     CDS             9707..10639
                     /locus_tag="VFH96_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6973247.1"
                     /translation="MIKSLIVLVAVLTISANAPAQQPSLQELVNRAAKTTLERFADKK
                     LQENELSITLIDLRDTKRPITASFRGNERVYPASVVKLFYLVATQRWLEDKKIEQTPE
                     LTRAVRDMIVDSSNEATQYVLDVLTHTTGGYELPPKEMEEWQYQRNAVNRYYASLGYT
                     NINVNQKTFCEDAYGRERVSRGPNGENRNKLTTDATARLLMEIVAGKIANAARTAAMM
                     ELLKREYTGTSSDTDDQGRGFTGIALQGREGFRLWSKAGWTSTTRHDVAYVEMPDGGK
                     FVLATFTSNHSNDREIIPTVARVVIEGLKDVKSQ"
BASE COUNT         2397 a         3227 c         2874 g         2230 t
ORIGIN      
        1 tccgttccgt aaagggacga cttcagttcg actcggctac ggcatctatc atgaacaggt
       61 gtcgatcagc gccgcggagc tgcttgcgtt gaatccgcct tttcaggaga cggcgacaca
      121 aactttaacg agtatggacc agccgattgc gccaggatct gcaattccgg tggtggcctc
      181 ggccactccg gcttcgattc gcgctatcca gccgcatttc ctaacgcctt acatgcagca
      241 atggtcgtta gacgtgcagc atcagattgg aaacaaaacc atcttcacac ttggttacta
      301 cggttccaag ggaacgcacc tcaacggcaa caccgaatac aacgatctgc cggtaggctt
      361 cgccattaat tcacaatgtg ctaccgggac cgcggcattg caggatgccg gtgtggttac
      421 cacgcagtgc atgacgcctg gtacagcgct gactgcaacg ccaacgatct tcgatcaaat
      481 ccggcccttc cgtggttatc gatcgatcaa cgtgctcgaa acgcgatata actcgaatta
      541 tcacagcctg caggcctatc tgcagcatcg cttctcgggc gcttcgcagt ttaatttcgc
      601 gtatacctgg tcgaagaacc tgactgataa ccagacgtct tccgtcaatg ctgcgccgca
      661 ggatgtgaac aacatccgag cagaatacag ccgggcaatt ctcgatcgcc gtcacatagt
      721 gagtgccaac tggatttatg agttgccctt cttcgccaag tcgaacgggt taacccatac
      781 ggtcctcgga ggatggcaag tgtccggcat cggcagctac tacacgggtc tgccgtttac
      841 tgtggcgagc gccacctacg ataccgccgg aatcggattc atcccggcta tcgtcgctgg
      901 tggtcgaccg cgactgctat gcgatccgaa cgagaacgcg ccgcacacgg tcgaccagtg
      961 gttcaatggg gcctgctttg ccaagcaaac tcctgccggc gccaccggta tactcaatgt
     1021 gcctggcgat gcttcgcgcg gatcggttaa tggtccaccg acgacgcgag tggatttcac
     1081 tttggccaag tccttctctt tcagtgagag cacctcgatt caattccgag ccgaggcgtt
     1141 caatgttttc aatcacacga acttccgaaa cttgagcgtg agcagagcga tcacaagtga
     1201 agctctgttt gggtcggtga cgagtttccg tgatccacgc gtcctgcaat ttgggttgaa
     1261 gttcctgttc taagtttcaa ctcatttgtc actaaaaatg cccgcattgt aactgctaca
     1321 atgcgggcat taacttatga agacactgtt actgaaaaat gcgaacgtcg tgttggccga
     1381 tcgagaggta caggcttctg ttctcatcga aaacggcaga atagtttcgc tcgaagcggg
     1441 tgagggcgat gaagagatcg acctcgcagg cacaacgctg ctgcctggtt ttatcgatgt
     1501 tcacattcat ggcgccgcgg gcgttgatgt catggatgcg acagcggcgg gtttgcgtga
     1561 agtgtcggaa tatctcgcgt cgcagggtgt gacgagctgg gtgccgacgt ttgtcccggc
     1621 cagtgatgag cattatgcga gcgcgattgc ggtgatttca gcagcgatga acgggccggg
     1681 agcgcgcgtg ttgggcgtgc attacgaagg accgtttgta aacacggcgc agtgtggggc
     1741 gctgcatacg gagtacttca aaagctattc ccagcctgaa gaactcgaat tcctggtggt
     1801 gccgccggcc ggtgtgcgca tgatcacgat ggcgcctgag gttgcaggtg gagttgagct
     1861 cgtgcgcgag ttgaagcggc gcggttgggt gatctcgatc ggccacaccc gcgcgagctt
     1921 ggaagtgctt gatgatgctt gcgaggccgg agcgcaccac atgacgcact tcatgaatgc
     1981 gatggcgcct ttacatcatc gcagtcccgg gccgatcgcg tggggattgt cacgcgatga
     2041 tgtgaccgtt gatgtcattg ccgacggtat tcatctcgat ccattcatgc tgcgtttgtt
     2101 gctgaagtta aaaggggcaa atgggatcag tctgatcagc gacgcgattg ccgcagcagg
     2161 gaagggagac ggtgattacg agatctgggg cgaaacgatt agcgtaaagg acggacgcac
     2221 cgcgaatgcg cacggtagta tcgccggctc cgtgatcagc atgctcgacg ccgtgcggtt
     2281 gctgcactcg cttggcgtgt cttatgtcga cctcgcccgc atggcctcat cgaatccggc
     2341 gcggctgttg ggtgtcgata acgattgcgg ctcgatcgaa gtcggcaaac gcgccgatct
     2401 ggttgctttg gacaacgaca acaacgcgaa actaactctg atcggcggta aatccgttcc
     2461 aaatccgcat taatccgtag ccaccaatga tgaccaacgc gaacccaatc agtacgtgca
     2521 atacgtcgtc aacgtacgtc cagtggaaat actcgatcgg cgtcagcccc acaacactcg
     2581 ccagcgcctg gtaaagatcg atcaatccga atccgaagtt gaacaagctc gccagcacgt
     2641 ctttattcgt catcaccagc agcagaccga gcgcgccgaa gaagatatga aacaaattgt
     2701 acggcgctgc tccgctcgtg agactgtatc gctcaggtat gatgaacccc gcgatgccga
     2761 tcagtatcaa gacgggtgcg aagatcgtga gggtcttttg attgagtgtt ttattctcca
     2821 ttactcgtaa taaccggcgg cttcataagc ctggtccacg atctcaatgg gatggcagat
     2881 gcgcagtttc attcccgaca agcacgcgcc cgcgccgatg tgcatctggc agccgggatt
     2941 gccggtcgcg agtataccag cacccgtctc tttaatgtgg tctaactttt cattcagcac
     3001 gcgctgcgac atctcgggct ggagcagatt gtagacgccc gctgcgccgc aacatctctc
     3061 cgtgccgttg agtggtacaa aatccttacc ggtaaccgat cgcagcatac gttctgaggc
     3121 ttcgccactg tgctgcccgt acatgaggtg acaagaggtg tcgtaagtca cggtctggcc
     3181 ggtgggacta ggcgcggttc gcatctccgt cgtcgcgagt tgctggctta cgtcgcgcac
     3241 ccgcgcgctg aatttcatcg ctctttcatc gttcccaaac agatgcgcgt acgttttcag
     3301 catcgccccg cagccgcccg cgttcgtgat gatcggagcg tcagaatcga aagcctcgat
     3361 attccgtcgc gccagctcgc gcgcaccttc gaggtctccg gcatgtgcat gcaacgctcc
     3421 gcaacatact tgtttcgccg gcgtctcgac ctcgaactcg ttcgcttcca acacacgctc
     3481 ggttgcacga ttcacacgcg caaacaaccc ctcgccaaca caccccttaa acaacaacac
     3541 actctcagcg gctgctttat gttttccgtc gcgcctttgc gccgctgagg gagaaaccgc
     3601 tctcgagctc tcaagcagcg ccattccaaa ctcagcccgt cgcggcaaaa ccccggtctt
     3661 caacaacagc cgcgccagcc tcagatcgcg aaaaactcgc gacgccgcaa agaacatccg
     3721 caaccgccat ggcgacaacc aaacatgcct caacacaaac cgcaatagcc tgttagccag
     3781 atcagacttc ggctgcgcct ccagtaatac ctcacgactc acctcaagca gttgcccata
     3841 ctcaacaccc gccggacaca cctgctcaca cgcgcgacac cccagacacc gatcgatatg
     3901 tgtcgcaaac gcgctcgacg tcttctcgag tcgtccctcc gccaccgcgc gcatcagata
     3961 caaccgcccg cgtggcccat cattctcatt acccgtaatg acgtacgtcg gacacgcctc
     4021 gagacacagc ccacaatgaa tgcaggccag cagtttatcc tcttgtcgcg ccaggtttgt
     4081 cgacatttag gttatccaca gattacgcag attctattaa ataaatctgt gtaatctgtg
     4141 gatagagcaa tcagtatcca atcgccagcc catcgcttcg acggggatcg gtcgcgccga
     4201 gccgcacgcc ggtcttctcc tcgatcatga tcccttctgc atcaccgagg taacgcagct
     4261 tcgcgagttt gtgcccgcgt gctgccagcg ccttttgcgt gtcacctgat agaccaaacg
     4321 gttcaccgac gagctcatcc ggcagccact ggtgatgaat gcgcggaaag tcgatcgcct
     4381 gctgaatgtt catctcgtag tcgatcacgt tggtgatgac gcagagcacc gtgttgatga
     4441 tcgttggtcc acccggactg ccaacggtga accataagct gccatctttt cgcagcacga
     4501 tcgtcggcgt catcgcagag agcggacgct tcttcggcgc gacagcattg cgctcaccct
     4561 ggatcaatcc gtacatgttc ggcgtgccgg gctttgctgc gaagtcgtcc atctcgtcgt
     4621 tgagcagcag gcccgtgccc ttcgcaactg ctgctgagcc gtaagagtta ttgagcgtgt
     4681 aagtgttcga gaccgcgttt ccttccgcat cgacgaccgt gaaatgcgtc gtctcgtccg
     4741 attcataacc cgccggacgg cctgcgcgaa tctcttcact cgtcgacgcg cgttcggtgt
     4801 tgattgtctt acgcaaggtg gccgcgtacg ccttgtcaat cagacccggt actggaacct
     4861 tcacgaaatc gctatcgccc atgtactccg cgcgatccgc gaaagcgcgc cgcatcgcct
     4921 cggccatgag gtgataacga tcggaagagt ttgcctcgag cttttgcaga tcgaagcctt
     4981 cgagaatgtt gagcatctgg atcagcacgg cgccgcccga cgaaggcggt ggcatcgaga
     5041 tgatctcgta accacgatag ttaccgcgca cgggcgtgcg ttctttcgcg acgtaacccc
     5101 gcagatcgtc catcgtcatc aaaccgttgt tgcgtttcat gtcggcgacg atcagacgcg
     5161 ccgtctcgcc ctggtaaaac tcatttggac caaaccgttg cagccgcgca aacgtggcag
     5221 cgagctcagg ctgtcggaag agttcgcctt cctggtaaaa ctttccgcct tttaaataga
     5281 tgcgtttcgt ttcggcgtac ttctcgagat attcccggtt cgaatagagc gagcgggcca
     5341 gttcgtttgt aacgacgaaa cctctaccgg cgagttgccg cgcgggttcg acgagttgtg
     5401 cccaggtgag cttgccggag ccgtacttcc tgagcgcgag ttccatcccg cgcaccgtac
     5461 cgggaacacc cgcggcgcga tagccgacga gcgatccacc ttcgccttcg atcaggttgc
     5521 cgttcttgtc gagatagacg ttgcgatgag cagcatcagg cgccatctcg cggtaatcaa
     5581 tcgcggtcgt tctaccatct ttcagtcgga tcatcatgaa gccgccgccg ccgagattcc
     5641 ccgccgccgg atgcgtgact gcgagcgcaa acccaacagc aatcgcagca tcgacagcat
     5701 tcccaccacg cttcatgatc tccacgccga cacgcgacgc cacttcattc gtcgacgcca
     5761 ccatcccatg ccgtccccgc accggctctc gcgcgatagt caacggaaca ttcagcgcca
     5821 cgaccagcag cagaacgatt actttgattt gtgtcttcat cgtcttaatc cgtcaaaaat
     5881 ccgcgtcaat ccgcggctaa aatattcttt ggatcgagtt gtctcttgat cctttgcatc
     5941 aataatttcg cagtaccgga aggttcttta cgcgccctgc ctgacaacgc tcgcgcagcc
     6001 gcaatcctcc gccaaagctc agcatcatcc atcacgatct cagcgttctg caacagtttc
     6061 aacgcctgtt caatctgaaa tgctacgcct ttttcgttac ccgcaaagcg aacatgcagt
     6121 acatcatcaa cgatctccaa cgctaccgga aataagcgcg cgtccaaaat cgcccgggca
     6181 cttccagtac caatcactgt cgcctcgcgt gccggtcgcg gtcgcagctt aaagttcagt
     6241 tcagtgatga ttccgagact gccataactg ccggtaaaca acttgcacag atcgtaacca
     6301 gcaacgtttt tcaccacgcg tccgccggct ttgatcgtgc tgccatctgc gaggacaacc
     6361 ttcataccga tcaccgatcc gcgtggtcgt ccataaccaa actgttgcgg accgccgatg
     6421 cccgtcgcaa ccacaccgcc gagcgtcgcg cgaccatcgt cgggcggatc cagcggtagc
     6481 cactgaccgt tctccgcgag ctttgcattg aaatcagtta gcgttacgcc gctctgagcg
     6541 atcgcaatca ggtctgcggg ttcgtgttcg atgatccgat tcagacgacg cgtgctgaca
     6601 atgagattcg ctcttgaact gacccccatc atcccaccgg ccggcacgac ggtccaacgt
     6661 tcagatgagg cgagtctcaa cacgtctgaa atctcttctg ctgatgctgg tgagactacg
     6721 atcgttccgt taagcgaagc gatgttcacg tcaccaacga tctgggccaa caacgttaca
     6781 gcccggtcag gattgaactc ggttggtgtt gccacttcga cttcaggttt ctcaacaact
     6841 ggcgcgatca cgggctgcat ctgagccgca gacccattat ctgacgcaac tgccttcgcc
     6901 tcaccacaac cgcgcagcat cggcacgatc ttccccggat tgcaaagccc gagcggatcg
     6961 aacgccgccc gcacactcaa catcgcattc atgtctgcgt cagaaaacac gagcggcaac
     7021 aactcgcgct tgtcgagccc cacaccatgc tcacccgtaa tcgttccgcc cgctgccaca
     7081 cacacttcca tcaactcgcg tccggcatct ttcacacgcc gcacctcgtc ggggaaacgc
     7141 gaatcaaaac agatgagcgg gtgcaggttg ccgtcgcccg catgaaacac attcgcgatc
     7201 cgcagttgat agcgcgacgc gatgtcgtaa gcggcgccga gcacctgcgg cagacgcgac
     7261 cgcggcacca ccgcatcctg gatcatcgaa tcaggcgaga tacgaccaat cgcaccaaac
     7321 gcccctttgc gtgccgccca tagttttttt cgctcagact catcgcgcgc gtgtcgacaa
     7381 ctgcgcgcac cgtattccat acaaatgctc gtgaccttgt gcgcttcgtc gtcgaggccc
     7441 gcttcgatcc cgtccaactc gatcaataat gccgcgcctg catcgggtgg cagtcccgcc
     7501 gcaaacacac tcgcctcgac cgcgcgaata atttcgtggt ccatcatctc gagggctgcg
     7561 ggcattacgc ctgccgcgat gattgcagac actgcatggc tcgcgtcgtt tacttccata
     7621 aactcagcca gcagcgtgcg cacggcgggc ggcgactgcg ccaacctgag cgctgcttca
     7681 gtagcgatac cgaatgtgcc ttccgagccg atgaacgtgc cgagtagatc gtaacccggc
     7741 gaatcaattt caagatcgac aacatcgccg ccggcaagca cgacgcgaca accgagtacg
     7801 tgatctgttg tcgtgccgta cttcagacag tgaatgccgc ctgcgctctc agcgatgttg
     7861 ccgccgatcg tgcagctcgg ttggctcgac ggatccggaa cgtaatacaa accgaacggc
     7921 gccacggctc gcgagacgtg caggttgacg acacccggtt ggaccacggc gatacggttc
     7981 tcggtatcga tgcgaagaat cttgcgcatg cgcgccatct cgatgaccac gcctgagttg
     8041 agcgcgagtg cgccgccgga aaggcctgtg cccgcaccgc gcggcgtgaa aggaacgttg
     8101 gcgcgcgcga gctcgcgcat gatggcggag gtttcgtcag ttgaagcagg gaagacgacc
     8161 gcgcgcggcc gatgtttgtg ctgcggcagg ccgtcgcact cgtagacgag tagctcgtca
     8221 ggtttggaga gcacattctc gcgcccaacg attgcggcca ggttgttgat tagggtgtcc
     8281 aagccatcaa aatatatcag taatacttag ccacggataa cacggattgg ttacggatct
     8341 aatccgtcaa caatccgtgt aatctgtggc taaactcttt tataaaacca gtatgccaaa
     8401 tagatcagca gaccggttcc ggcggcgtat gctgtggctt tcacttcgtc aaatcttgcg
     8461 ttacgtagca gccaaacaat caataacaac gagagaatcg cgacaaccgt cccgcccggc
     8521 aatcgaaaca tcgccgccgg cgcgttccgt cgcgcacgaa ataccggcaa cgcgagacac
     8581 gtcgcggcgt acgtcacgag ccgcgcgatc gtgctgatcg tgagtgccgc cacaaacgag
     8641 ctcttcaagg tcagaaacaa catcaatccc gccgtgatca ggatcgacac atacggcgtg
     8701 aagaacttcc tgtgtatgct gcccacgaac gccggcagtt gcttctgctc cgccatcgca
     8761 aacggaagcc gcgaccccga caataagaga atattcaagt tgcccgtgat cgaaatgatc
     8821 gcgcccgcgg agatgatcgc tccgcccgcc gcccccagaa actgcgaacc cgcatccgcc
     8881 agcggcttcg tcgactgcgc gagtcccggc aacgtgccga cgcaaaccac ctggatcatg
     8941 atgtaaagaa tcgcgaccac gagaatcgcg atcagtaacg cgcgtggcaa gctcttctgc
     9001 ggatcgcgca cttcaccggc gggaatcgtc gccatctcaa acccggtaaa tgcataaacc
     9061 agcaacagga ccgatttcga aaaatctgcg gtcgtcgggc ttggtccaaa ctcgaaagcc
     9121 tgcgggttca ggaaaaatag tccggcagcg ataaatatta ttatcggcac gagcttgccg
     9181 accgtgaatg cattactgac gatcgccgcc tgccggattc cgagcaggtt gatagcagcg
     9241 agcgcgatga cgataagcac gatgatcgac gcgcgccaga gcggagtcgt cgccggcagc
     9301 cagaagtaac tcacgtaatt gatcagcagg ttgcagttcg ctgcaaatgc cgtcatacgc
     9361 gcgagccaga tgagccaacc gaccccgaac gcgaccgccg gttggaacgc ctcgcgcgcg
     9421 tagaggtatg gaccaccagt ctcgtcgaaa cggctgctga cctccgcaaa gcaaagtatg
     9481 atgagcgcga cgacgagggc gcaggcgacg aaggcgatga gactgtaagt gccgattaat
     9541 ttgtaaacat tggaaggaag accaaagatg ccggcgccga taatgccgtt gatcgtgatg
     9601 gcgacgaggt cccagcgacg tatgccgcgg accaggtccg cctcaggttg gttctgcacg
     9661 aaaagcttat aacaaaatct gcgtgatctg tgtattctga gtgcctatga ttaaaagcct
     9721 aatcgttttg gtagcggtct taaccatctc tgccaatgct cctgctcaac aaccgtcatt
     9781 gcaggagctc gtcaaccgcg ccgcgaagac gactctcgaa cgattcgccg acaagaaact
     9841 ccaggagaac gagctgtcaa taaccctcat cgatctgcgc gatacgaagc gtcccattac
     9901 cgccagcttt cgcggaaacg agcgcgtgta tccagccagt gtcgtaaagc ttttttatct
     9961 cgtcgcgacg cagcgctggc tcgaagacaa aaagatcgag caaacaccgg agctgacgcg
    10021 cgccgtgcgc gacatgatcg tcgactcatc aaacgaagca acgcagtacg tactcgacgt
    10081 gctcacgcac acgaccggcg gttacgagct gccgccgaaa gagatggagg aatggcaata
    10141 tcagcgcaac gctgtcaatc gctattacgc atcgctcggc tacaccaaca tcaacgtgaa
    10201 tcagaagacg ttctgcgaag atgcatacgg tcgcgagcgc gtgtcgcgtg ggccgaacgg
    10261 cgagaatcgc aacaagctta cgaccgatgc gactgcgcgt ttgctgatgg agatcgttgc
    10321 cggaaaaatc gcgaacgcgg cgcgtactgc ggcgatgatg gagcttttga aacgcgaata
    10381 cacgggcacc agtagcgaca ccgacgatca gggacgcggg ttcacgggca tcgcgttaca
    10441 gggtcgcgaa ggcttccgat tgtggtccaa agcaggttgg accagcacca cgcgacacga
    10501 tgtcgcctac gtcgagatgc cggacggcgg caagtttgtc ctggcaacgt tcaccagtaa
    10561 tcattcaaac gaccgcgaga tcattcccac cgttgcgcgc gtcgtgatcg aagggctgaa
    10621 agacgtcaag agtcagtagc tgaacttgga tcgggtcagt accacctcgc ggtagcgggt
    10681 gggtcaacga taggctcaaa ttaattggaa tttcgcacgc tgatcatt
//
LOCUS       DASSFQ010000129         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1690809, whole genome shotgun sequence.
ACCESSION   DASSFQ010000129 DASSFQ010000000
VERSION     DASSFQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690809"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="thrB"
                     /locus_tag="VFH96_04450"
     CDS             <1..721
                     /gene="thrB"
                     /locus_tag="VFH96_04450"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET6973248.1"
                     /translation="NLIYRAMSFAARNEGVTLPPVELVAHNEIPLASGLGSSAAAIVA
                     GIKLAGLITEKEISDQSILNYATEFEGHPDNVAASLHGGFLASCIGTDKTVISAKFDW
                     PSKIRVVVVSPHSQLPTHVARAALPRTVTRLDAVYNLQRAALFAAAIAQQRYDILWEA
                     MHDRLHQPQRESLVPGLAAALALPRMPGLLGIALSGAGPSIVALVESNEEEIGQRIAK
                     CFKVGSTVRTLDVDNEGCRIN"
     gene            complement(714..2021)
                     /locus_tag="VFH96_04455"
     CDS             complement(714..2021)
                     /locus_tag="VFH96_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6973249.1"
                     /translation="MKTQGHLLRVLGVGFGLAVAIGNTIGAGIFKAPGSIAEQLPAPT
                     PFLLVWLAGGLYAFLGAISIAELGTMIPRSGGQYVFSRYALGEYAGFIVGWSDWISTC
                     GSTAAVSLVVGTFAVALFPALNGKAVPIAVVVAIVFALLQWRGIAWGSNIQNITSLLK
                     ALAFIALIVAAFIFGGEGSLTSSQVSAMPVITISAIVISLQAAIYTYDGWTGVIYFSE
                     EVDNPGKDIPHALFGGVAAIAAIYLLVNIALLYVLPISQIAGKEFAAGQAATVIFGRY
                     GDTIFRCLTIVSMLSGVNALHLMATRVVFAMSRDGLFIKKAADVNKGGTPTVSLLLSA
                     LVALLFIVFGEKFETVITVLAFFFVVNYILSFVSLFVLRQREPEKPRPYRAWGYPVTP
                     ALALIGSVLFLAGAIRADTKNSVYALILLAVSYPVFWVTKRLS"
     gene            complement(2068..3189)
                     /locus_tag="VFH96_04460"
     CDS             complement(2068..3189)
                     /locus_tag="VFH96_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET6973250.1"
                     /translation="MREIVSQYDPNLALDLASTIPASWYTNSDFYQLELKTVFEQSWQ
                     LAARADQVAEPGRYVTTDIAGEPIVIVRGNDRVLRGFFNVCRHHAAAVMTEPEGKAAH
                     LRCPYHGWTYSLEGELKGTPDFSGVCDFDRAKNGLVSIEIAEWENWIFVKTVQNVPES
                     IDEFLGDELLAQISPLQLTELHWFERRHYQFDCNWKVFVDNYLDGGYHVPYLHKGLDS
                     VLDYSNYQIENGEHYCLQWSPIVSEGAEVQTGAVRKGDRALYYWIYPNFMINWYDGVM
                     DTNLVIPRGVDQTEVIFDFYFPDVVSEEARERNRASVAVGQRIQDEDVAICKSVQRGL
                     GSRAYHAGRLSVRREAGEHLFHRLLHRDVRRILAADERG"
     gene            complement(3213..>3799)
                     /locus_tag="VFH96_04465"
     CDS             complement(3213..>3799)
                     /locus_tag="VFH96_04465"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET6973251.1"
                     /translation="VWDALMERGRAFDIHPAGMLALDVARVEAGLLLIDVDFNSSKKA
                     LVDEQRYSPYEMGLGRLVNLDKNRFVGQAALIEEQKRGHAREIVGLEIDWPQVESIYE
                     EVGLPPAVSPMASRVAVPVYKEGMQVGKATTTTWSPTLKRMIALATVNRAYTRPGTRL
                     QFEITVEAVRHQVRATVAKTPFFNPKRKTATPVV"
BASE COUNT          883 a         1127 c          993 g          796 t
ORIGIN      
        1 taaccttatt taccgcgcga tgtctttcgc cgcccgaaac gaaggtgtga cgttgccgcc
       61 ggtcgagttg gtcgctcaca acgaaattcc actggcgagt gggctgggca gcagcgccgc
      121 ggcgatcgtc gcgggtatta agctcgcggg tctaatcacc gagaaagaaa tttccgatca
      181 atcgattctg aattacgcga cggagtttga aggccatccg gataacgtcg ccgcgtcgtt
      241 gcacggcggt tttctcgcaa gctgtatcgg caccgacaag actgtcattt ccgcgaagtt
      301 tgattggccg tcgaagattc gcgtcgtcgt cgtttcacct cactcacaac tgccgacaca
      361 cgtcgcgcgc gcagcgttac cccgcaccgt cacccgcctt gacgccgttt acaaccttca
      421 gcgcgcggcc cttttcgctg ccgcaatcgc gcaacagcgt tacgacatcc tctgggaagc
      481 aatgcacgat cggctacatc agccgcagcg tgaatcactt gttcccggac tcgcagccgc
      541 gcttgcgctc ccgcgcatgc cgggtctgct gggaatcgcg ctgagtggcg ctggtccaag
      601 tatcgttgct ttagttgaga gtaacgagga agagattggt cagagaatag cgaagtgttt
      661 caaagtcggc tcgactgtca gaaccctcga cgtcgacaac gaaggctgtc gcatcaactg
      721 agtcgcttcg ttacccagaa caccggatag ctcaccgcca gcagtatcaa cgcgtaaaca
      781 ctattcttcg tatccgctcg aatcgctccc gcaagaaaca acaccgatcc aatcaacgcg
      841 agtgccggcg tcacgggata accccacgcg cgatacggcc gcggcttctc aggctcgcgt
      901 tgccgcagca cgaagagtga aacaaacgag aggatgtagt taaccacgaa gaagaatgcg
      961 agcactgtga tgacagtctc gaatttttcg ccgaagacga tgaacagtag cgcgacaaga
     1021 gcactgagaa gaagcgagac agttggagtt ccgcctttat ttacatcggc cgctttcttt
     1081 ataaacagcc cgtcacggct catcgcaaac acgacacgcg tcgccatcag gtgcagcgcg
     1141 ttgacgccgc tcagcatcga cacgatcgtc agacaacgaa agatcgtgtc gccgtaacgg
     1201 ccaaagatca ccgtcgccgc ctggcctgcc gcaaactcct tgcccgcgat ttgcgaaatc
     1261 ggcaacacgt aaagcagcgc gatgtttacc agcagataaa tcgccgcgat agccgcaacg
     1321 ccgccgaaca gcgcgtgagg aatatctttt ccgggattat caacttcttc cgaaaagtag
     1381 atcacaccgg tccaaccgtc gtacgtgtaa attgccgcct gcagcgaaat gacgatcgcc
     1441 gatatcgtga tcaccggcat cgccgaaact tgtgaactcg tcagcgagcc ttctccacca
     1501 aagatgaacg ccgccacgat cagcgcgata aaggcgagcg ctttgagcaa gctggttatg
     1561 ttttgaatgt tgctgcccca ggcgattccg cgccactgta aaagcgcaaa gacaattgcc
     1621 accaccactg cgatgggtac ggctttgcca ttgagagcgg gaaagagcgc gacagcgaat
     1681 gtgccgacga cgagcgacac cgctgcggtt gacccgcacg tagagatcca gtcgctccat
     1741 cccacgatga agcctgcgta ctcacccagc gcataacgtg aaaagacgta ctgtccaccg
     1801 gagcgaggaa tcatcgtgcc gagctcagca attgagatgg cgccgaggaa tgcgtaaaga
     1861 ccaccggcaa gccaaacgag caggaagggt gtcggcgcgg gtaattgttc ggcaatcgag
     1921 ccgggcgcct taaaaatccc cgccccgatc gtgttgccga tggccacggc gagaccaaac
     1981 cccactccga gtacgcgcag caggtggcct tgagttttca tcaaaatatt tagccgcgga
     2041 tatacgcaga taaacgcaga tctgcgttca tccgcgttca tccgcggcta aaattctcct
     2101 cacatctcta tgcaacaaac gatggaataa atgctcaccc gcttcgcgtc tcactgacaa
     2161 tcttcccgca tgataagcac gtgaacccaa tccgcgctgc actgacttgc agatcgccac
     2221 atcttcatcc tggatgcgct ggcccaccgc cacactcgct cgattccgct cccgcgcctc
     2281 ttcactcacg acatccggaa agtaaaagtc gaaaatcact tccgtctggt ccacaccgcg
     2341 cggtatgacg aggttcgtgt ccatcacgcc gtcgtaccag ttgatcatga agtttggata
     2401 aatccagtaa taaagcgcgc ggtccccttt tctcaccgcg cccgtctgca cttcagcgcc
     2461 ttcactaacg atcgggctcc actgcaaaca atagtgctcg ccgttctcaa tctggtaatt
     2521 gctgtagtcg agcacactgt cgagtccttt gtgcagataa ggcacgtgat aaccaccgtc
     2581 aagataatta tcgacaaaca ctttccaatt gcagtcgaac tgataatggc ggcgttcgaa
     2641 ccaatgcagc tcggtcaact gcaatggact tatctgcgcg agcagttcgt cgccgagaaa
     2701 ctcatcgatc gattcaggca cattctgaac ggttttaacg aatatccaat tctcccattc
     2761 ggcaatttcg atcgacacga gcccattctt agcccgatcg aaatcgcaga cgccggaaaa
     2821 atccggtgtg cctttcaatt caccttcgag cgagtaggtc caaccgtgat acggacaacg
     2881 aaggtgcgcc gcttttcctt cgggctcagt cattacagcc gcagcgtgat gacggcagac
     2941 gttgaagaat ccgcgcagca cgcgatcgtt tccacgcacg atcacgatcg gctcaccggc
     3001 gatgtcggtc gtgacatagc ggcccggctc ggctacctga tcggcgcgtg ccgcgagttg
     3061 ccacgattgc tcgaaaacgg tttttagctc gagttgatag aagtcagagt tggtgtacca
     3121 cgacgcaggg atcgtcgacg ccagatcgag cgccaggttc ggatcgtatt gactaacgat
     3181 ctcacgcaat tggagctccc gtaattgagc gttcatacga caggcgtggc tgtcttgcgt
     3241 ttcggattga agaacggcgt ttttgcgacg gtcgcgcgca cttgatgacg tacagcttcg
     3301 accgtgattt caaattgcaa tcgtgtgccc ggacgtgtgt aagcgcgatt gacggtggcg
     3361 agcgcaatca ttcgcttcaa cgttggcgac caggtggtgg tggtggcttt accgacctgc
     3421 atgccttctt tgtacactgg cacggcgacg cgcgatgcca tcggtgaaac tgccggcggc
     3481 aagccgactt cttcataaat gctctcgacc tgtggccaat cgatctcaag gccgacaatc
     3541 tcgcgcgcgt gaccgcgctt ctgttcttcg atcagcgccg cctggcccac aaagcgattt
     3601 ttgtcgagat tcacgaggcg tccaagtccc atttcgtaag gcgagtaacg ctgttcgtcg
     3661 acgagcgctt tcttgctgct gttgaaatcg acgtcgatca atagcaatcc tgcttcgacg
     3721 cgcgccacat cgagcgcgag cattccggcg ggatggatat cgaaagcgcg acctctttcc
     3781 atgagcgcgt cccaaaccc
//
LOCUS       DASSFQ010000130        14231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1089294, whole genome shotgun sequence.
ACCESSION   DASSFQ010000130 DASSFQ010000000
VERSION     DASSFQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14231
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089294"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFH96_04470"
     CDS             complement(<1..524)
                     /locus_tag="VFH96_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET6973252.1"
                     /translation="MTFINYASREINCKIVYYGPGLCGKTTNLQYIFDSTAPQARGKL
                     ISLATETDRTLFFDFMPLELGTVRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQEERMDANIESLYNLEENLQSQGYDLLKIPYVLQLNKRDLGNIVPAEDLAAELQK
                     KGEPVIEAVASTGQG"
     gene            complement(534..1004)
                     /locus_tag="VFH96_04475"
     CDS             complement(534..1004)
                     /locus_tag="VFH96_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET6973253.1"
                     /translation="MAEPILLREQQYHQIKAVLARLRMDSSAKVVFLVDKDGQEIASQ
                     GEVGNLDTTSLASLAAGNVAATGGMAALIGEKEFPTLSHEGEKESIHISVVGRLLLVV
                     VFDERSSLGLVKLRSKQVSHQLAKMFEEITSADYDDVDSPFAGITDEDIDSLFS"
     gene            1361..2203
                     /locus_tag="VFH96_04480"
     CDS             1361..2203
                     /locus_tag="VFH96_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973254.1"
                     /translation="MSSSLTKGLRRSTSRSKAKPAAKSAKRAVKAKSPSKPKATRAAA
                     AKSSARSAAKARPAKKTAAKSKPKTRKAAPKPVARKAAAAKKPVVSRVKTTQKQAAVR
                     AIRPTPAPPPPPRPPTQDESAALVAFERAHKEFARGRFAEARNQFRILVDKYSQVAEV
                     AARARTYLAIAESRLRTENILPRDADSLYDRGVIELNRGEYVAAQEMFERALKREPEA
                     AHIHYGLAATRARLGSIDLALESLQRALDLQPNLRVRAQHDHDLNPLRNDPDFDRLIF
                     SNPS"
     gene            2345..3364
                     /gene="yvcK"
                     /locus_tag="VFH96_04485"
     CDS             2345..3364
                     /gene="yvcK"
                     /locus_tag="VFH96_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HET6973255.1"
                     /translation="MQQTIPTGGQKLVAIGGGTGLSCLLGGLKSLVGNTNDEMWIESL
                     SAIVTVSDDGGSSGRLRDELQMLPPGDIRNCMIALSEDSKLMSRLFSYRFRGDGELGG
                     HSFGNLFLAALTELTGDFTEAIRVSSEILASKGHIYPATISDVRLAAELEDGSVVRGE
                     TQISASKLPIRSLRLEPERCLPLPEALKAIRAADIITVGPGSLYTSILPNLLVSQVAR
                     VIGQSRATKIFINNLMTQPGETDGYSAREHLETIKKYAPEIHFDFVVVNNRRISVEQA
                     ERYAAEGAYQIGIDDGIDDALDRTTEIVRTDLLYDGEKVRHNSDRLAHVVIDCRKQAL
                     APVLA"
     gene            3357..4766
                     /gene="glmU"
                     /locus_tag="VFH96_04490"
     CDS             3357..4766
                     /gene="glmU"
                     /locus_tag="VFH96_04490"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET6973256.1"
                     /translation="MPKIFREMSNNNTSSRPLNVLIMAAGLGTRMKSRRAKVLHELGG
                     APLIAYVVRAAQALEPRSIITILGHQAEEVERAVLAEVGELASFVIQERQRGTGDAVE
                     SARRVLEASDSLVLVLSGDVPMIRVETLQKLIEYHHNTGAACSILSVRLENPTGYGRI
                     IRDENGAFRKIVEQRDATEEERLVREINSGIYCFEAKALFDALRKVEPKNDQGEYYLT
                     DVAEIILARGETVSVYLINDPREVSGVNTRAELAEFENLVRRSAIRRLMIESGVTFID
                     PSHAYISAEAQIGCDTIIHPNVAIEGKTVIGEGCVIRAGARITNSRLGDNVTIKDHSV
                     IVDSQIESNCSVGPFAHLRMNALLEEKATVGNFVEVKKSRLKRGAKAMHLTYLGDATI
                     GERTNIGAGTVTCNYDGVNKHETIIEDDVKIGSDTMLVAPVRVGARSKTGAGSVVTRD
                     VPPDSLVAGVPAEVKKKLK"
     gene            4846..6708
                     /gene="glmS"
                     /locus_tag="VFH96_04495"
     CDS             4846..6708
                     /gene="glmS"
                     /locus_tag="VFH96_04495"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET6973257.1"
                     /translation="MCGIVGYVGNKQVVPLIIDGLRKLEYRGYDSAGIAVVNEDHDLE
                     IRRAEGKLRNLEETIRLSPLDGTYGIGHTRWATHGRPTEENAHPHRDCTGRVVVVHNG
                     IIENYLQLKERLRKSDHHFVTETDTEIMAHLIEEYLKTDSSFEQAVRSAVSELRGIFA
                     LAIINADEPDTIIAVRQGPPVVIGLGDREFFVASDIPPILQHTRDVFFLGDGEIAVIN
                     RDAVRVTDFEGNSVQPTIQRITWDPIMAEKGGFKHFMLKEIYEQPRSVRDTVQGRISL
                     DSGQVFLDEMSNITEADFKRFTSIKIAACGTSWHAGLAGKYMIEQLARVPVDVDYASE
                     FRYRSPVMDENTLLLVISQSGETADTIAALREAKELGAKVLAICNVQGSMIVREADGT
                     ILTHAGPEIGVASTKAFTAQMIALYLLGLYLGNLRKVLSVDEARFHAQELAELPVKLE
                     HLLNDSDGIEELSKEFFRSTDFLYLGRGINFPVALEGALKLKEISYIHAEGYPAGEMK
                     HGPNALIDERLPVLFINTREEGNRASELRYEKTHSNIVEVKAREGIVISVLTEGDSIS
                     SVVSDHVIEIPAASDLLSPILSIIPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(6772..7872)
                     /gene="ada"
                     /locus_tag="VFH96_04500"
     CDS             complement(6772..7872)
                     /gene="ada"
                     /locus_tag="VFH96_04500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HET6973258.1"
                     /translation="MLNNDQLWQAVVAKDAHFDGQFVFAVSSTGVYCRPSCPSRRAHR
                     ERVRFFDLPEAAEQAGFRACLRCQPKRAHVLDPQIELVQRVCRFLDETESDTPKLAEL
                     ASHAGVSVFHLQRTFKRVMGISPRQYVAARKFGNFKALVRKGESVTNSLYESGFNSSS
                     RLYEYASEELGMTPATYSRGGRGVNISYTIVDSTMGRLLVAVTERGVCAVRMGDDDAE
                     LEKDLREEFPHAQITRDDSALRETVEKILNHLDRNEPRLDLPLDIRATAFQRQVWEKL
                     RAIPYGQTVSYADVAKSLGKPGAVRAVGRACATNPVALVIPCHRVVREDQSLGGYRWG
                     LDRKKKLLERERGRPFLSSESALEEAGGRRRE"
     gene            8010..10349
                     /locus_tag="VFH96_04505"
     CDS             8010..10349
                     /locus_tag="VFH96_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6973259.1"
                     /translation="MHARVDSDFLSQLNEQQREAVLATEGPLLILAGAGSGKTRVIAH
                     RIAYLIAEKKIPSWNILAVTFTNKAADEMRQRVQRLLHTQERNDLPLVSTFHSLCVRI
                     LRQDIEKLNDKYTRRFTIYDQDDSIRLIKQAIRDLGYDEKYVAPRATQSAISSAKNRG
                     EDPTAFAARAEYQDERRAIMAKVYEMYEARLHSNNALDFDDLLIKTVRLLRDVDEVRE
                     KYNNKFRYILVDEYQDTNSLQFALISLITQKSQNIAVVGDEDQSIYKWRGADISNILN
                     FEKHFPKTKTIRLEQNYRSTQTILDVAGAVVKNNLERKGKSLWTSNPRGGRVGYYQAF
                     DAEAEARWVAHKVVEHLREERDLRAAVLYRTNSQSRVFEEAMRRTGLQYNIIGGFSFY
                     ERMEVRDIIAYLKLALNPNDSIALGRVINSPPRGIGKTTLEELERRAKDYELSWWETI
                     SNMIEQPGSLGPRAISALKSFRNIINKLAQLAVTPDESIKEPVSDVVRAAIVETGYEL
                     SLRGERTDEAEGRLENLQELVNAAVDYDDQGIEGLREFIDHSALVSDQDEYKRDAPVT
                     LMTVHAAKGLEFPLVFVVGLEEGLFPHSRAASDRAELEEERRLAYVAMTRAERYLYVT
                     HAMKRRVYGEELASEPSTFLNEMPDELLEDLSLGKSWLSFKGGSKQIDYEQGEYKKER
                     KKYTGKTYDSADSIADFFKQRNRQMGQTSGSVPKISKPVAASTSNEITPGSYVKHSKY
                     GRGLVLRREGVGESAKLTVSFPGFGQKKLVEKYAGLEKA"
     gene            complement(10405..12495)
                     /locus_tag="VFH96_04510"
     CDS             complement(10405..12495)
                     /locus_tag="VFH96_04510"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HET6973260.1"
                     /translation="MRLRRLFSALLLALFGLQTLHITALADEGMWPFNNVPRAEIKKK
                     YGFEVTDEWLNKVRLASVRFNNGGSGSFVSPNGLVLTNYHIVEDIVGEVSTPEKDLAK
                     EGFVARTRAEEIKAPSLELNVLQSIEDVTSRVNGAVKDGATDAEAFAARRAEIAAIEA
                     ESTRATSLRSDVVTLYQGGQYNLYRYKKYTDVRLVFVPEFQAAFFGGDPDNFNFPRFN
                     IDMALVRVYENGQPVKPENYFKWSTAGVKEGNLVFVTGNPGSTSRLNTVAHLEELRET
                     SIPIILRLLERRETMLKKYMALGEEQTRQGQNELNSVQNSLKVYRGQLKGLKDPALLA
                     RKQKEEQTLRKAIADNPERQKMYGEAWDAIAKAHQTYPTYIKERRIFEQLGGFNSQYF
                     TFARMLVRLAEESTKPNNERLLDYTDARRASLELMLYSPAPIHNDFEELKLADSLGFM
                     VELLGADHPLVKQVLAGKTPEARADEMIAGTKLGDVAVRKELAAGGKAAIDASTDPMI
                     VVARMIDAKAREVRKRYESELTGVERANYAKIARALFETEGTKLYPDATFTLRLSYGA
                     VEGYMENGQKVPAFTTLGGLFARSDKFKHEFPYNLPERWVAKKSAMNLNTPFNFVSTN
                     DIIGGNSGSPTINQNGELVGLIFDGNIQSLVGDFMYDPSVNRAISVDSRGMLEVLKKV
                     FGATEIVSELTATS"
     gene            complement(12632..13720)
                     /locus_tag="VFH96_04515"
     CDS             complement(12632..13720)
                     /locus_tag="VFH96_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973261.1"
                     /translation="MNSLSARVISITGMLGVLLLRWACYPASEITYSNPQDATSSQWI
                     IEYSTTDNKLHLTMRYRRTRDTGFSYNDSGFNVTLDQLTGLTRDQLLSQTGTNVRFQL
                     KRDAGSFDFEGWFKQGNGSGHFAFTPNSSFNSELGRLGFGRATDEQLLAFAMTDTGLA
                     FINELKAQDYDTTTVEQLVRMAHHGVSIEYLQGLKALGYTVKSTDLVVRMKDHGVSLN
                     FIKELAALGYNNMSSEDLIRTRDHGVTAKFINEFMAAGYERAPLDQWIMLRDHGVTTE
                     FGSELKNLGYDRQSLEDLRRMRDHGVTASFIKEIKDVGYSNTSVEQLVRLKDHGVSAA
                     YIKRMKERGYADLTLDEYIRLRDRGERE"
BASE COUNT         3198 a         3930 c         4001 g         3102 t
ORIGIN      
        1 ccctgacccg tcgaagcaac cgcctcaatc acaggctcgc cttttttctg cagctcagcc
       61 gccaaatctt ccgccggtac gatattgccc aaatcgcgct tgttcagttg cagcacgtac
      121 gggatcttca gcaaatcgta gccctgcgat tgtaaattct cctcgaggtt gtaaagcgac
      181 tcgatattgg catccattcg ctcttcctgc gaatcggcca cgaacaccac tccatccaca
      241 cctttcaaaa tcaacttgcg cgacgcatcg taaaaaacct gacccggcac ggtgtacagg
      301 tgaaagcgtg ttttgaaacc gcgaactgtc cccagctcga gcggcataaa atcaaagaac
      361 aacgtgcgat cggtctcggt cgcgagactg atcagcttgc cgcgcgcctg tggcgcggtc
      421 gaatcaaaga tgtactgcaa gttggtcgtc ttaccacaca aacctgggcc gtagtaaacg
      481 atcttgcagt tgatttctct cgaagcgtaa ttgatgaagg tcatagcgtg ccgttagctg
      541 aaaagactgt cgatgtcctc gtcggtaatg ccggcgaagg gtgaatcgac gtcgtcgtag
      601 tctgcagaag taatttcttc aaacatcttc gccaattgat gagagacctg cttactccgg
      661 agtttcacaa gtccgagact ggagcgctcg tcgaagacga ccacgagcag cagtctgccg
      721 actacggaga tatggatgct ctctttttcg ccttcgtgcg agagcgttgg gaattccttt
      781 tctccgatca gtgcagccat tccccccgtc gccgcgacat taccggcagc gagggaagcg
      841 agactggttg tgtcaagatt tcccacttca ccctgggatg cgatctcctg accgtcctta
      901 tctacgagaa agaccacctt ggctgaggag tccattcgca atcgcgccag gacagccttt
      961 atctggtggt attgctgttc acgcaaaagg attggttctg ccattgtgtg cgactcttta
     1021 ttgaaggccg gggcgggatg tttcctggag agctaaacgt gaaaacgcct gttccgcgga
     1081 ttgcaagttt ttgaaactaa aagaggttac ttattcggtt ggggcagttt gccgaggtgg
     1141 attaagcctc ccataagccg aaaagcactt taacatagcc aacgatggcc atcaacattt
     1201 ttctttgttg aaacttgtgt cggaaaagtg gactgatttt cggtggttaa agcacccgca
     1261 aatacttgtc tccgttgatt tagaggtagg tctatggtaa gctgcggtcc aattttacgg
     1321 ctgctcgctt tcaggtgagc agccttttga gaatccgccc atgagtagca gtctcacaaa
     1381 agggttgaga cggtcaacat cgcgttcaaa agctaaacca gccgcgaaaa gcgcgaagcg
     1441 cgctgtaaaa gcgaagtcac cttccaaacc caaagccacc agagccgccg ctgccaagtc
     1501 gtctgcgcga tcggcagcca aggcgcgccc ggccaagaag actgcggcga agtccaaacc
     1561 caaaacccgg aaggcagcgc caaagcctgt ggctcgcaaa gctgcagccg ctaagaagcc
     1621 tgtcgtctca agggtcaaaa cgacgcagaa gcaagctgcg gttcgagcga ttcggccgac
     1681 gccggctccg ccaccaccgc cgagaccgcc gacacaggat gagtcggcag cactcgtggc
     1741 gttcgaacgc gcccataagg aatttgctcg tggccgtttt gcagaagcgc ggaaccagtt
     1801 tcgaattctc gtagacaaat actcgcaggt agcggaagtc gcggctcgcg cgcgcacgta
     1861 tctcgcgata gcggaatcgc ggctgcgaac tgaaaacatt ctcccacgtg atgccgactc
     1921 gctttacgat cgcggcgtca tcgagcttaa tcgaggtgag tatgtagcgg cgcaggaaat
     1981 gttcgaaaga gcgctcaagc gtgagccgga agcggcgcac attcactatg gactcgccgc
     2041 cacgcgcgct cgactcggtt caatcgacct ggctctggaa tcactccagc gcgcactcga
     2101 cctgcaaccg aacctgcgtg tacgtgcaca acacgatcac gacctgaacc cactgcgaaa
     2161 tgaccccgat ttcgatcggc tgatctttag caacccctcc tagttagttt tctctgtgcc
     2221 acctggaata gaacaagtca ggaatcgggg cttgcccccg ctcattcaat ccaagccaca
     2281 gcgggagcaa gcacctcttc ctgacttgtt ctattcagag gtatgatttg atccgaggct
     2341 aagtttgcag caaacaattc ccacaggcgg tcagaagctc gtagccattg gcggcggcac
     2401 tggtctctcg tgtttactcg gcggcctgaa gagtcttgtc ggaaacacca acgatgagat
     2461 gtggatcgag agcctctccg cgatcgtcac ggtctccgac gacggcggta gctccggacg
     2521 cctgcgcgat gagttgcaaa tgctcccgcc cggcgacatc cgcaactgca tgatcgcgct
     2581 gtcggaagac tctaaactga tgtcgcgcct tttcagttat cgctttcgcg gtgacggcga
     2641 gctcggcggt cacagtttcg gcaatctctt tctcgccgca ttaactgaat tgacaggaga
     2701 cttcactgaa gccattcgcg tctcttccga gattctcgcc agcaagggcc atatctaccc
     2761 ggccacgatc agcgatgtca gactcgcagc ggagctcgaa gacggatccg tggtgcgtgg
     2821 tgagactcaa atctccgcgt ccaaactccc gattcgcagt ttgcgactcg agccggaacg
     2881 atgtcttccc ttgcccgaag ctctcaaagc gattcgcgct gccgacatca tcactgttgg
     2941 accaggctcg ctctacacga gcatcctgcc gaatctgctc gtgagccagg tcgcgcgtgt
     3001 catcggccag tcgcgcgcca cgaagatctt catcaacaat ctgatgactc agccgggtga
     3061 aactgacggc tacagcgcgc gcgagcactt ggagacgatc aagaagtacg cgcctgagat
     3121 tcatttcgat ttcgtcgtcg tgaacaaccg gcgcatcagt gtggagcagg ctgagcgcta
     3181 cgctgccgag ggcgcgtatc aaatcggcat cgacgacgga attgacgacg cactcgaccg
     3241 aacaactgag atcgtccgta ctgatctgct ttacgacggc gagaaggtcc ggcacaattc
     3301 ggatcggctt gcacacgtcg tgatcgactg ccgcaaacaa gcactcgccc cggttcttgc
     3361 ctaaaatctt ccgagagatg tcaaacaaca acacatcgtc cagaccactg aacgttttga
     3421 tcatggcggc cggtctcggc accaggatga agtcgcggcg cgcgaaggtg ctgcacgagc
     3481 tgggcggcgc gccgttgatc gcgtacgtcg tccgggcggc gcaggcgctc gagccgcgct
     3541 cgatcatcac gatcctcgga caccaggctg aagaagttga gcgtgcggtt ttggcggaag
     3601 tcggcgagct cgcgagtttt gtgattcagg aaagacaacg cggcaccggc gatgcagttg
     3661 agtcggcgcg gcgcgtgctc gaagcctcag actcgctcgt gctcgtgttg tcaggcgacg
     3721 tcccgatgat tcgtgtcgag acgttgcaga agctaatcga gtatcaccac aacactggcg
     3781 ctgcttgttc gattctgagt gttcgtctcg agaatccgac cggctacgga cgcatcattc
     3841 gcgacgagaa tggcgcgttt cgaaagatcg tcgaacagcg cgacgctact gaagaggagc
     3901 gtctcgtgcg cgagatcaat tccggcattt actgttttga agcgaaggca ctgttcgacg
     3961 cgttgcggaa agtcgagccg aagaacgatc agggtgagta ttacctgacg gacgtggccg
     4021 agatcattct cgcgcgcggt gagaccgtga gcgtttatct aatcaatgac ccacgcgaag
     4081 tctcgggagt gaacacgcgc gccgagctgg cggagtttga gaacctcgtg cggcgctcgg
     4141 cgattcgacg gctgatgatt gaaagtggcg tgacgttcat cgatccgtcg catgcgtaca
     4201 tcagtgccga agcgcagatc ggatgcgaca cgatcatcca tccgaacgtc gcgatcgagg
     4261 gaaagacagt gatcggcgaa ggttgcgtga tccgcgccgg cgcgcgcatc acaaactcgc
     4321 gtctcggcga caatgtcacc attaaagatc actcggtgat cgtcgattcg cagatcgagt
     4381 caaactgttc cgtcgggcca tttgcgcacc tgcggatgaa cgcgttgctg gaggagaaag
     4441 cgaccgtcgg caacttcgtc gaggtgaaga aatcgcgact gaaacgcggc gcgaaagcga
     4501 tgcatctcac gtatctcggc gacgcaacga ttggtgaacg tacaaacatc ggcgcgggaa
     4561 cagtcacctg taactacgac ggcgtgaaca agcacgaaac gatcatcgaa gacgacgtca
     4621 aaatcggttc cgacacgatg ctcgtcgcac ccgttcgtgt cggcgcccgc tcaaaaaccg
     4681 gcgcgggctc agtcgtcacg agagacgttc cgccggattc gttagtggcc ggcgtgccgg
     4741 cagaagtaaa gaagaaactt aaatgagcca caaaaagcac aaaaggcaca aaaggttttt
     4801 ttgtgctttt tgtgcctttt gtggctaaag aggagtaaag atcgaatgtg tggaattgtt
     4861 ggttacgtag gcaacaagca ggtggtgccg ctcatcatcg atgggttgcg taagctcgaa
     4921 tatcgcggct acgactcagc cggcatcgcc gtcgtcaacg aagatcacga cctcgagatt
     4981 cgtcgcgccg aaggtaaact gcgcaacctc gaagagacga tccgcctgag tccgctcgac
     5041 ggcacgtacg gcattggcca cacgcgctgg gccacacatg gccgcccgac cgaagagaac
     5101 gcgcacccac accgcgactg caccggacgc gtcgtcgtcg ttcacaacgg catcatcgaa
     5161 aactatctcc agctcaaaga gcgactgcgc aagtccgatc accacttcgt caccgaaacc
     5221 gacacagaga tcatggccca cctgatcgag gaatatctca aaactgattc ctctttcgaa
     5281 caggccgtgc gttcggcggt gtctgagctg cgcggcatct tcgccctcgc gatcatcaac
     5341 gctgacgagc ctgacacgat cattgccgtg cgccagggac ctcccgtcgt gatcggtctt
     5401 ggcgatcgcg aattcttcgt cgcgtcagac attccaccga tcctgcaaca cacacgcgac
     5461 gtcttctttc tcggcgacgg cgagatcgcc gtcatcaatc gtgacgccgt gcgcgtgaca
     5521 gactttgaag gcaattcggt gcagccgacg attcagcgga tcacgtggga tccgatcatg
     5581 gccgaaaagg gcggcttcaa gcatttcatg ctcaaggaga tttacgagca gccgcgttcc
     5641 gttcgcgaca cggtgcaggg acgaatctcg ctcgatagcg gtcaggtgtt tctcgacgag
     5701 atgagcaaca tcaccgaagc cgatttcaaa cgtttcactt cgatcaagat cgcggcgtgc
     5761 ggcacgtcgt ggcatgcggg actcgcgggc aagtacatga tcgagcagct cgcgcgcgtg
     5821 ccggtcgatg tcgattacgc gtcggagttt cgatatcgca gtccggtaat ggatgagaac
     5881 acgttgttgc tcgtgatttc tcaatcaggc gagacggccg acacgattgc ggcattgcgc
     5941 gaagcgaaag aactcggcgc gaaggtgctt gcgatctgca acgtgcaggg ttcgatgatc
     6001 gtgcgtgaag cagacggcac gattctcacg cacgctggac cggagattgg agtcgcttcg
     6061 acgaaggctt ttaccgcgca gatgatcgcg ctttatctgc ttggtttgta cctcggaaat
     6121 ctgcgcaagg tgctttcggt tgacgaagca cgttttcatg cgcaggagct ggccgagctg
     6181 ccggtgaaac tcgagcatct gctcaacgat tcagacggca tcgaagaact ctcgaaagag
     6241 ttcttccgct cgactgactt tctctatctc ggtcgcggca tcaatttccc ggtcgcgctc
     6301 gaaggcgcgt tgaagttgaa agagatctct tacattcacg cggaaggtta tccggcaggt
     6361 gagatgaagc atgggccaaa cgcgctcatc gacgagcggc tgccggtgct cttcatcaac
     6421 acgcgtgaag aaggcaatcg cgcttcagag ctgcgttacg aaaagacgca ttcgaatatc
     6481 gtggaagtga aagcgcgtga gggtattgtg atctcggtgt tgactgaagg tgattcgatc
     6541 tcgtcagtgg tttcagatca tgtgatcgag attccggctg cgagtgactt gctgtcgccg
     6601 atactctcga tcattccctt acagttgttg gcatatcaca ttgcggtgcg tcgcgggtgt
     6661 gatgttgatc agccacgaaa tctggcgaaa tcagtcacag tagaatagcc tctttgcggc
     6721 acccctcgag ggcaaacggc ggcggcacta catcccccaa gagtgccttg gctactcacg
     6781 ccgtctgccc cccgcctcct ctaacgcgga ttcagacgat aaaaagggcc gcccgcgttc
     6841 acgttcaagt aattttttct ttcggtccaa accccagcga tagccgccaa ggctctgatc
     6901 ttcgcgcacc acacgatgac aaggaatcac gagtgcgacg ggatttgtcg cgcaggcgcg
     6961 gccgactgca cgtacagcgc cgggcttgcc gagtgatttg gcgacatcgg cgtacgaaac
     7021 tgtctggccg taaggaatcg cgcgcagctt ttcccaaacc tgtcgctgaa aggctgtggc
     7081 tctgatgtcg agtggcagat cgagtcgcgg ctcgttccgg tcgaggtgat tcagaatctt
     7141 ctcgaccgtt tcgcgcaagg cggaatcgtc gcgtgtgatc tgcgcgtgtg gaaattcttc
     7201 gcgcaggtct ttctcgagtt cagcatcatc gtcgcccatt ctcactgcac acacgccacg
     7261 ctcagtcacc gcgacgagca aacgtcccat cgtcgaatcg acaatcgtgt aactgatgtt
     7321 tacgccgcga ccgccgcgac tgtaagttgc gggcgtcatg ccgagctctt ccgacgcgta
     7381 ttcgtagaga cggctgctgg agttgaaacc tgattcgtac agcgagttag tcacggattc
     7441 tcctttgcgc acgagcgcct tgaaattgcc aaacttgcgc gcggcaacgt actggcgtgg
     7501 gctaatcccc atcacgcgct tgaatgttcg ttgcaggtga aagacgctca caccggcatg
     7561 cgatgcgagc tcagcgagtt tcggtgtgtc gctttcggtt tcgtcgagaa agcgacagac
     7621 acgttgcacg agctcgatct gcggatccag gacgtgtgcg cgttttggtt ggcaacgcag
     7681 gcacgcgcgg aagcctgctt gttctgcggc ttccggcaaa tcgaagaacc ggacgcgttc
     7741 gcgatgggcg cgacgcgagg ggcacgatgg gcgacaataa actccagtcg agctgacggc
     7801 gaatacgaat tgaccgtcga agtgtgcgtc tttcgcgacg actgcctgcc atagttgatc
     7861 gttattcagc atgtggggat tttagatctc gcagtatcct ggatctaccc gattcttgcg
     7921 gtcaaattag ccacggatta cacggattac atacacggat ttattcagtg taaatccgtc
     7981 tttttttaat ccgtgtaatc tgtggttaaa tgcatgcacg cgtggattcg gatttcctgt
     8041 cacaactaaa tgaacaacaa cgcgaggctg tgcttgctac cgaaggaccg ctcctgatcc
     8101 ttgcaggcgc cggctcgggc aagacgcgcg taattgcgca tcgcatcgct tatctcatcg
     8161 ccgagaaaaa aatcccgagc tggaatattc tcgccgtcac gttcacaaac aaagctgccg
     8221 atgagatgcg ccagcgcgtg cagcgattgc tccacacgca ggaacgcaat gacctgccac
     8281 tcgtgtcgac gtttcacagt ctctgcgtgc gcatcctgcg acaggacatc gaaaagctca
     8341 acgacaagta cacccgccgg ttcacgatct acgatcagga cgactcgatt cgtttgatca
     8401 agcaggcgat ccgcgatctc ggttacgacg agaagtacgt ggcgccacgc gcaacgcagt
     8461 cagccatcag ctccgccaag aatcgtggtg aagatccgac ggcgtttgcc gcgcgtgcgg
     8521 agtaccagga cgagcgccgc gcgatcatgg cgaaggttta cgagatgtac gaagcgcgcc
     8581 tgcacagcaa caacgcgctc gatttcgacg atctgctgat caagaccgtg cgactgctgc
     8641 gcgacgtcga tgaggtccga gaaaagtaca acaacaagtt tcgttacata ctcgtcgacg
     8701 aataccagga caccaactcg ctccagtttg cgctcatcag tctgatcacg cagaagtcgc
     8761 agaacatcgc ggtcgtcggc gacgaagatc agtcgatcta caagtggcgc ggcgcagaca
     8821 tcagcaacat tctcaacttc gagaagcatt ttccgaaaac taaaaccatt cgcctcgaac
     8881 aaaactatcg ctcgacgcag acaattctcg acgttgcggg cgcggtggtg aagaacaatc
     8941 tggagcgcaa aggcaagagt ctctggactt caaatccgcg cggtggtcgc gttggttatt
     9001 accaggcgtt cgacgcggaa gcggaagcgc gttgggtggc ccacaaagtc gtcgagcatc
     9061 tgcgtgaaga gcgcgacctt cgcgccgctg tgctctatcg caccaactcg caatcgcgtg
     9121 tgtttgaaga agcgatgcgt cgcacgggct tgcagtacaa catcatcggc ggtttctcgt
     9181 tttacgagcg catggaagtg cgtgacatca tcgcgtatct gaagctggcg ctgaatccaa
     9241 acgactcgat cgcgctcgga cgcgtgatca atagtccgcc gcgcggcatc ggcaagacta
     9301 cgctcgagga actcgagcgg cgcgcgaaag attacgagct gtcgtggtgg gaaacgatca
     9361 gcaacatgat cgagcagccg ggaagtctgg gaccccgagc aatcagcgcg ctcaagagtt
     9421 ttcgcaacat catcaacaaa ctcgcgcagc tcgcggtgac accggacgag tcgataaaag
     9481 agccggtctc tgacgttgtg cgtgccgcga ttgtcgaaac gggttatgaa ctgtcgttac
     9541 gtggcgagcg gaccgacgaa gccgaagggc gactcgagaa cttgcaggaa ctcgtcaatg
     9601 ccgcggtgga ttacgacgac cagggaatcg aagggctgcg cgagtttatc gaccactccg
     9661 cactcgtttc cgatcaggac gagtacaagc gcgatgctcc ggtcacatta atgactgttc
     9721 acgcggcgaa gggactcgag tttccgctcg tgtttgtcgt cggactcgaa gaaggtttgt
     9781 ttccgcattc acgcgcggcg agtgatcgag cggaattgga agaagagcgc cggctcgcgt
     9841 acgtggcgat gacacgtgcc gagcgttatc tctacgtgac acatgcgatg aagcggcgtg
     9901 tatatggtga agagctggct tccgagccgt cgacgttttt gaacgagatg ccggacgagt
     9961 tgctcgaaga tctcagtctt ggtaagtcgt ggctttcgtt caaaggcggc tcaaagcaga
    10021 tcgattacga acagggcgag tacaagaaag agcggaagaa gtacacgggc aagacttacg
    10081 acagcgccga ttcgatcgcg gactttttca aacaacgcaa ccggcagatg ggccagacat
    10141 cagggagcgt cccgaagatt tcgaagcccg tcgcagcttc aacatcgaat gaaatcacgc
    10201 cgggttctta tgtgaagcat tcgaagtacg ggcgcgggct cgtgttgcga cgcgaaggcg
    10261 tgggcgagtc tgcaaagcta acggtgagtt tcccgggatt tgggcagaag aaactggtag
    10321 agaaatatgc tggcttggaa aaagcgtaac acggataaca cggattagat ccggatctaa
    10381 tccgtgttat ccgtggctaa agttctagct ggtggcagtc aactccgaga cgatctcagt
    10441 agccccgaag accttcttca agacttcaag cattccgcgc gagtcaaccg aaatagctcg
    10501 attcactgac gggtcgtaca tgaagtcgcc gaccagcgat tgaatgttgc cgtcgaagat
    10561 cagcccgacc agttcgccgt tctgattgat cgtcggtgag cctgagtttc cgccgatgat
    10621 gtcgttcgtc gacacgaaat taaacggcgt attcagattc atcgcactct tcttcgccac
    10681 ccaacgctcc ggcaggttat atggaaattc gtgcttgaac ttgtcggagc gagcgaatag
    10741 accaccgagc gtcgtgaacg ccggaacctt ctggccgttc tccatgtaac cttcaaccgc
    10801 accgtatgaa agtcgcagcg tgaacgtcgc atcgggatag agcttcgttc cctcggtctc
    10861 gaaaagcgcg cgtgcaatct tcgcgtagtt ggcacgctcg acgcccgtca attcactctc
    10921 gtaacgcttg cgaacctcgc gcgctttcgc atcgatcatc cgggcgacaa cgatcatcgg
    10981 atcggtcgaa gcgtcgatcg cggctttgcc accggcggcc agttctttac ggaccgccac
    11041 atcacccagc ttcgtgcccg cgatcatttc gtcagcgcgt gcttcaggag tcttgcctgc
    11101 gagcacttgc ttcacgagcg ggtgatcggc gcccagcagc tcgaccatga aaccgagcga
    11161 gtcagcgagt ttcaactcct cgaagtcgtt gtggatcgga gccggtgaat acaacatgag
    11221 ctcgagggac gcacgacgag catccgtgta atcaagcaag cgctcgttat tcggcttggt
    11281 gctttcctcc gccagccgta cgagcatgcg tgcgaaagta aagtactgcg aattgaagcc
    11341 gcccagttgc tcgaagatac gacgctcttt gatgtaggtt ggataagtct gatgagcttt
    11401 ggcaatcgcg tcccaggctt cgccgtacat cttttgccgt tccggattgt cagcaatcgc
    11461 cttgcgcagc gtctgctctt ctttctgctt acgagccagc aacgcgggat ctttgagtcc
    11521 cttaagctgc ccgcgataaa ccttcaaact gttctgcacg ctgttcaatt cgttctgccc
    11581 ctggcgcgtc tgctcttcac ccagcgccat gtattttttc aacatcgtct cacgtcgctc
    11641 caacaagcga agaatgatcg gaatgctggt ctcgcgcagt tcttccaggt gcgcaaccgt
    11701 gttgagacgc gaagtcgaac ctggattgcc ggtcacgaaa acgagattgc cttccttgac
    11761 gcccgcggtg gaccacttga agtaattctc cggttttact ggctgaccat tttcgtaaac
    11821 gcggaccaga gccatgtcga tgttgaagcg tgggaagttg aagttgtcag gatcgccgcc
    11881 gaagaacgcg gcctgaaact caggcacgaa cacgagacga acgtcggtgt atttcttgta
    11941 acgatagaga ttgtattgtc cgccctggta aagcgtgacg acgtcagagc gcaagctggt
    12001 agctctcgtc gactccgctt cgattgccgc aatctccgcg cgtcttgctg caaacgcttc
    12061 ggcgtcagtc gcgccatcct tcacggcgcc gttcacacgc gacgtaacgt cttcaatact
    12121 ctgcagcacg ttcaactcga gactcggcgc cttgatctct tcagcgcgtg tgcgagcaac
    12181 gaatccctct ttcgcgaggt ccttctccgg cgtgctcact tcgccgacga tgtcttcaac
    12241 gatgtgatag ttcgtcagca cgaggccgtt cggcgatacg aatgagcccg agccgccgtt
    12301 gttgaagcgc acggaggcga gccggacttt attcaaccat tcatcagtaa cttcaaagcc
    12361 gtattttttc ttgatttctg cgcggggcac gttgttgaac ggccacatgc cttcgtcggc
    12421 cagcgcagtg atatgcagtg tttgtaagcc aaagagcgcc agcaggagcg cagaaaaaag
    12481 tcgtcttaat ctcatttcaa tcctcaataa acagtcttat gggcagagac aacacagatt
    12541 acaacgaggg gaggggatct gtgaaactgt gtgatgcggc gatagtggcc caaggttcgc
    12601 gcgctgttca aacgccgccc gggcgactca cttactcgcg ctcgccacgg tcgcgcaatc
    12661 ggatgtattc gtcgagggtg aggtcagcgt agccacgctc tttcattcgt ttgatgtacg
    12721 cagcgctgac gccgtgatcc ttgagtcgca cgagttgttc gacgctcgtg ttggaatagc
    12781 cgacgtcttt gatctccttg atgaaggatg ccgtgacgcc gtgatcccgc atgcgtcgca
    12841 gatcttccag cgattgacga tcgtagccga gattcttcaa ctcagaaccg aattcggtag
    12901 tcacgccgtg atctctcagc attatccact ggtcgagtgg agcgcgctcg tagccggcag
    12961 ccataaattc gttgatgaac tttgccgtga cgccgtgatc tctggttctg atgagatcct
    13021 cggaagacat attattatag ccgagagcgg cgagctcctt gatgaagttt agagatacgc
    13081 cgtgatcttt catgcgcacg accagatccg tgctcttcac cgtgtaacca agcgctttca
    13141 gaccctggag gtattcgata ctcacgccgt gatgcgccat gcgcacgagc tgttcgacag
    13201 tcgtggtgtc gtaatcctga gctttcaatt cgttgatgaa ggcgaggcct gtgtcagtca
    13261 tcgcgaaagc gagaagttgt tcatcggtag ccctgccgaa accgagcctg ccgagttcgg
    13321 agttgaaaga gctgttgggc gtaaatgcga agtggccgga gccgtttccc tgtttgaacc
    13381 agccttcgaa gtcgaatgag ccggcgtcgc gcttgagctg gaagcggacg ttggtgccgg
    13441 tttgggacaa taactggtcg cgcgtgaggc ccgtaagctg gtcgagcgtg acattaaatc
    13501 cgctgtcgtt atagctgaag ccggtatcgc gtgtgcgccg gtagcgcatt gtgaggtgca
    13561 gcttgttgtc ggttgtcgag tattcgatga tccattggct gctggtggcg tcttgcgggt
    13621 ttgaataggt gatttctgaa gcgggataac aggcccaccg cagcaacaaa acgccgagca
    13681 taccagtgat cgagattact ctcgcgctta aggaattcat gttgtcctcc ggatgtggat
    13741 ctcctggagt gcgctaacca ctcaacgcac tccaggattt tcctaagaaa gaaagcagac
    13801 tacctgtttg cttgtgaggt aaacaccacc cggtaaagat ttgtgactaa tgattggagc
    13861 gtttgtttgg ctgttcgcca gacatcggcg cgggtttgtt agtggcctcg ctgccgcact
    13921 gacggcaaag gtagctatcc tgtttctgtg cggccggaac cagccaaagg catccacacg
    13981 aatcacataa aatcgtcgat ccaagcagcc actcgttgaa tcgtatctgt ttttctaccc
    14041 tcatcgtccc ccctgatcgt ttgccgggat attttgaagc gctagggttt ctaaactaca
    14101 aacgacgcga gagtttcaat aaattgtcgc ggcttattgt caggaagaat ttagccgttt
    14161 gaaaagaaca cgtcaggaag aggggcttgc ccccgcctgt ccaatccaag cagcggcggg
    14221 ggcaagcccc t
//
LOCUS       DASSFQ010000131         7265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1826086, whole genome shotgun sequence.
ACCESSION   DASSFQ010000131 DASSFQ010000000
VERSION     DASSFQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826086"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1385
                     /locus_tag="VFH96_04520"
     CDS             39..1385
                     /locus_tag="VFH96_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973262.1"
                     /translation="MLSPASRHAFSIFLVVALALTAVFAQSKPGSQKQPALPQPSATP
                     PPEPPEDIETLKTETDLVTVPIIATNRDGLYITDLSQEEFKISEDGVPQEVAFFGKVA
                     APFHVVLMLDTSSSTKDHLRQIQNAAFTFVQQLQPVDRVKIISFDDKINDLNEFTSDR
                     ETLRAAINTTRPGEGTKVYDAFELAMNTLRKIRGRRAIVLFSDGMDWFSDKATFASTV
                     RYLDEEGVVVYPIRYDTRATTEKLAREQSDQVSLPTIGTIRQPPPSGTTAPTFPGGDG
                     VPTSGSQQKTGPFGLPSAGEIMRRRRDEERNRYPDPNGGPAPTGGPGDIPPDNRDRTI
                     PRVPGSEPTGTTVPSSRPEDASIERTLDMAYKTADSYLRELAEKSGGRLLRADTIASL
                     PDAFGQIAAELRTQYMLGYYPLNKNRDDRYRKIKVETTRKNVAVRARPGYIATAAK"
     gene            1549..2124
                     /locus_tag="VFH96_04525"
     CDS             1549..2124
                     /locus_tag="VFH96_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6973263.1"
                     /translation="MPDRNEAWELLCEYTKGDSLRKHALAVEAAMRACAKRYNEGHAD
                     EDEWGMVGLLHDFDYEMFPTADQHPFTGANILCGRGYSDRIIRAIMGHATYTGVPRDT
                     DMARALFATDELCGFLVACALVRPSRSLDDMEVSSVKKKLKDKAFARSVNRDDIKQGV
                     EELGVNIDDHIRFVIDALRPVQKEIGLNTIT"
     gene            complement(2117..2449)
                     /locus_tag="VFH96_04530"
     CDS             complement(2117..2449)
                     /locus_tag="VFH96_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973264.1"
                     /translation="MRLKLVIAASVLAAVVGAGICIALVLGVFSVKALSNPGLLVTST
                     LVLPIATIAFATIFVYRHTARRRKLQAFLTAIIAIILTLTFFIAAALVTGHYRPLEPR
                     PPAEHIAT"
     gene            2561..3481
                     /locus_tag="VFH96_04535"
     CDS             2561..3481
                     /locus_tag="VFH96_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET6973265.1"
                     /translation="MITRDSLLWLSQREGLKDFATRFGFFKKLTTRFVAGDSIDEVIP
                     FIRQLNSENCTASFDHLNESVGSAAEAEGEVAEYLNILAKIDESRIRSNVSIKLTQFG
                     LGLDPELAYRNARRIVEEAQRRGNFVRVDMEGSDVTQVTIDIFKRLRAEFGLNDVGIV
                     LQSYLYRTYADAQELVKLPARIRICKGAYSEPAEVAFPDKKDVDANYVKVMQLLLGSG
                     TYHGIATHDPKMIDATIDFVKREGIGKEKFEFQMLYGIRRDLQRQLARDGYNVRIYVP
                     YGKHWYPYFMRRLAERPANVWFVLKNLAKG"
     gene            3490..3969
                     /locus_tag="VFH96_04540"
     CDS             3490..3969
                     /locus_tag="VFH96_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET6973266.1"
                     /translation="MDWIWILWCILGAILIVAEIFTTGFVLLWFGVGALAAAFAGFIG
                     IESLAIQFAIFAVVSMALTAASRTIFVKYFSHEKTGQSLRSGVDALPGKIGTVVSSSR
                     GALQEGAVKVFGSTWTAYPAPGEPPLEAGERVCVESIEGASIYVKRIGGEPDWRVKQ"
     gene            complement(4023..4946)
                     /locus_tag="VFH96_04545"
     CDS             complement(4023..4946)
                     /locus_tag="VFH96_04545"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET6973267.1"
                     /translation="MIVELYAKSDVGRVRRGNEDNFLVLDLSTEQTWTGADGNPPAEN
                     LFRFNLGDKGLVLVVSDGMGGALAGDVASRMAVDSVRKMLVGEGDAVCDPDLDLVECL
                     KSATHYANTAIHLRSQEDSRCAGMGATFTGAAVRGDNLDLVQVGDSRGYVIRKDQIRL
                     ATKDQSLVQQLVDVGQISESEAETHMFRNVILQALGAQSEVQPVTGRIRLRQDDLVLL
                     CSDGLSGKLRAEDIQHIVVNSRNDLSKACDALIEEANNRGGEDNITVVIARFSGDDLE
                     APSTDRITIELPPLEEDKTLDDTYEADTEPH"
     gene            complement(5032..6171)
                     /locus_tag="VFH96_04550"
     CDS             complement(5032..6171)
                     /locus_tag="VFH96_04550"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET6973268.1"
                     /translation="MGFATTAIHTGSEPDETTGAVTVPIYQTSTYAQDALGKNKGYEY
                     ARTQNPTRSAVERNIAAIEGGRFGFAFASGMAAIDATLRLVKAGEHVIVSDNTYGGTA
                     RLFNRILANYNLTFDYVDTSDPLNVEAAIKPNTKMVFIETPTNPVMILTDLKEVSEIA
                     HRAGARVVCDNTFMSPYLQRPLEFGVDIVVHSTTKYLNGHSDGVGGVVVLNDEEDANW
                     IGFVQNSAGAILSPFDSWLVLRGTKTLALRMEQHDKTGRAVAAFLEEHPKVKKVYYPG
                     SASHPQHALARRQQHGFGGMVSFDVGSLENARKVLESVKLCTLAESLGGVETLISHPA
                     TMTHASVDEAKRQRLGITDGLVRISVGIEDTDDIVADLDQALGQL"
     gene            complement(6180..7130)
                     /locus_tag="VFH96_04555"
     CDS             complement(6180..7130)
                     /locus_tag="VFH96_04555"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET6973269.1"
                     /translation="MANANIRNVHRGVAESVLDLIGQTPLLHLSRFAQPPLAGIYAKI
                     EWLNPGGSVKDRAALGMILDAEEKGLLKPGSTIIEPTAGNTGIGLALVGVARDYRVIL
                     CVPHGFAVEKMKLIEALGGELEYVSNEAGMQGAIERCHQLAETIDDSFVPQQFENNAN
                     PCFHELTTAREIIEQMNGEIDAVVIGAGTSGTFCGVARAVRKHNQNALCVLVEPEGSI
                     YGGGQPGPKKVEGIGQRDFIPKIFDASLADEILMVQDDEVCAALKKIAKLEGLLGGWS
                     SGAAAAASYKIAERLGPGKRVVTVFPDGAERYRSQGIFDE"
BASE COUNT         1623 a         1967 c         2060 g         1615 t
ORIGIN      
        1 aaagttcggt tgacgggttt cgcgtgagga gggactgaat gctttcacct gcgtcgcgtc
       61 atgccttttc catttttttg gtggtcgcgc ttgcactgac cgctgtcttc gcgcaatcga
      121 agccaggtag tcaaaaacag ccagcgctgc cgcaaccctc cgcgacgcca ccgccagagc
      181 cgcctgaaga tatcgagaca ctcaagactg aaaccgatct cgtcaccgtc ccgataatcg
      241 caaccaaccg cgatggactc tacatcaccg acctgagtca ggaagaattc aaaatttcgg
      301 aagatggcgt gccgcaggaa gttgctttct tcggcaaagt cgccgctcca tttcatgtcg
      361 tgcttatgct cgacaccagt tccagcacca aagatcatct ccgtcagatt cagaacgcag
      421 cgtttacttt cgtgcagcag ttgcagcccg tcgatcgcgt caaaatcatc tcttttgacg
      481 acaagattaa cgatttaaac gagttcacat ccgatcgaga aactttgcgg gcggcgatca
      541 acaccacgcg accaggcgag ggaaccaagg tctacgacgc cttcgagctt gcaatgaata
      601 cattgcgtaa gattcgtggc cgcagagcca tcgtgctgtt tagtgacggc atggattggt
      661 tcagcgacaa agcgacgttc gcaagcacgg tgcgctatct cgacgaggaa ggcgtggtcg
      721 tctacccgat tcgctacgac acgcgcgcga ccacagaaaa actggcgcgc gaacagtcag
      781 atcaagtctc gttgccgacc attggaacta tcagacaacc accgccgagt ggaactactg
      841 cgccgacttt cccgggcggc gatggagtgc caacctcggg gtcgcagcag aagactggtc
      901 cattcggatt gccgtccgct ggggagatca tgcgccggcg ccgtgacgag gagcgcaatc
      961 gttatccgga tccgaatggt ggtccagcgc cgacaggtgg tccgggggac attcctccag
     1021 ataatcggga caggacaatt ccgcgcgtgc ctggttcaga gccgaccggt actacggtgc
     1081 cgagcagccg tcctgaagat gcatcgatcg agagaacgct ggacatggcc tataagacgg
     1141 ccgacagtta cctgagagaa ttggcggaga agtcaggagg acgattgttg cgcgcggaca
     1201 cgatcgcttc gttgcctgac gcgttcggac agatcgcggc tgagctgcgc acgcaataca
     1261 tgctcggtta ttacccactc aacaaaaatc gcgacgatcg ttaccggaaa attaaagtcg
     1321 aaacaacgcg aaagaacgta gcggtccgcg cccgccccgg ctacatcgcc accgccgcaa
     1381 aatagttaag ccacaaaaag gcacaaaaac ccaaaaaaga aagttcagag ttcaagcttt
     1441 agcttgatgt cacggctgca gcctgaaagc ggaactctag acttcttctc ttttgtgcct
     1501 tttgtgcctt tttgtggctt aactgatttt gtgctacaaa actcactcat gcctgaccga
     1561 aatgaagcct gggaattgtt gtgtgaatac acaaaaggcg atagcttgcg taaacatgcg
     1621 ctggctgtcg aggctgcgat gcgtgcgtgt gcgaagcgtt acaatgaagg acacgcggat
     1681 gaggacgaat ggggcatggt cggattgctt cacgacttcg actacgagat gtttccgacg
     1741 gccgatcagc atccattcac aggcgccaac atcctttgcg gccgcggtta ttccgatcgc
     1801 atcatccgcg ccatcatggg ccacgccact tacacaggcg tgccgcgcga tacagacatg
     1861 gcccgcgcac tcttcgccac tgacgagctc tgtggtttcc tcgtggcgtg cgcgctcgtg
     1921 agaccttcgc gtagtctcga cgacatggaa gtctcgtcag tcaagaagaa gctgaaggac
     1981 aaagcattcg cgcgatcggt taatcgcgac gacatcaaac aaggcgtcga agagttggga
     2041 gtcaatatcg acgatcacat acgtttcgtg atcgacgcgc tgcggccagt gcaaaaagag
     2101 attgggttga atacgatcac gtagctatgt gttcggcggg tgggcgcggc tcgagtggtc
     2161 tatagtgtcc ggtcaccagc gcagcagcga tgaaaaatgt aagcgtcagt atgatcgcga
     2221 tgatcgctgt gagaaatgcc tgcaacttgc gacgacgcgc agtgtggcgg taaacgaaga
     2281 tcgtagcgaa ggcaattgtc gcgatcggaa gtacgagagt ggaagtaacc agcagacccg
     2341 gattagacag ggccttgact gagaagacac ctaacacgag cgcgatgcag ataccggcgc
     2401 ccacaactgc cgccagcacc gaagcggcga ttacgagctt caatcgcatc cgggctggtt
     2461 cctcgttttg atgctcgtgt gcaaagctcc tccttggctg gatggagaaa ggcgccgcca
     2521 aggataccac agggacggac cagataacgg actgaaatag atgatcacaa gagactcgct
     2581 gctttggttg tcccaacggg aagggttaaa ggatttcgcg acacgttttg gattcttcaa
     2641 aaaactcact actcgtttcg ttgccggcga ctctattgac gaagtaattc cttttattcg
     2701 tcagctcaat tccgaaaact gtacggcgtc gttcgatcac ttgaacgagt cggttggcag
     2761 cgctgctgaa gctgagggag aagtcgccga atatctgaac attctggcga agatcgacga
     2821 gagtcgcatt cgctcaaatg tctcgatcaa gctcacgcag tttggtcttg gtctcgaccc
     2881 ggagctggcc taccggaatg cgcggcgaat tgttgaagag gctcagcggc ggggaaattt
     2941 tgtccgagtt gatatggaag gctccgacgt tacgcaggtg acgatcgaca tcttcaaacg
     3001 tctgcgcgct gaattcgggc tgaacgacgt cggcatcgtg ttgcagtcgt acctgtatcg
     3061 aacttacgcc gacgcgcaag aactcgtgaa actgcccgcg cgcattcgca tctgcaaagg
     3121 cgcttacagc gagccggcgg aagtcgcgtt tcccgataag aaagacgtcg acgcgaacta
     3181 cgttaaggtg atgcaactgc ttttaggaag cggtacttat cacggcatcg cgacgcacga
     3241 tccgaagatg atcgacgcga cgatcgactt cgtgaagcgc gaaggaatcg gaaaagagaa
     3301 gtttgaattt cagatgcttt acggcatccg gcgcgatctt cagcgacagc tcgcgcgcga
     3361 tggttacaac gtaagaatct atgttcctta cggtaaacac tggtatcctt acttcatgcg
     3421 caggttggcc gagcgtcctg cgaatgtgtg gtttgtgttg aagaacctgg cgaagggctg
     3481 agtcagtcga tggattggat ctggattctc tggtgcatcc tgggtgcgat actcatcgtg
     3541 gcggagatct tcacgacggg cttcgtgctg ctctggttcg gcgtcggcgc gctcgcggct
     3601 gccttcgcag ggttcatcgg gattgagagt ctagcgatcc agtttgcgat ttttgcggtc
     3661 gtgtcgatgg cgttgactgc cgcgtcacgg acgatcttcg tcaagtactt ttcacatgag
     3721 aagactggac aatcgctgcg cagtggtgtt gatgcgttgc cgggaaagat cggtactgtg
     3781 gtttcgtcga gtagaggtgc gttacaggaa ggcgcggtaa aagtgtttgg ctcaacgtgg
     3841 accgcgtatc ccgcgccggg cgagccgccg ttggaagcag gcgaacgcgt ttgtgttgag
     3901 agtattgaag gcgcgtcgat ttacgtgaag aggatcggcg gtgagcctga ctggcgagta
     3961 aaacagtagc cacggataac acggatttaa atccggattt aaaatccgtc taatccgtgt
     4021 tcctaatgtg gttcggtatc ggcttcgtag gtatcgtcga gtgtcttgtc ttcttcgagt
     4081 ggtggcagct cgatcgtgat ccggtcagtt gaaggcgctt ccaaatcatc accggagaag
     4141 cgtgcgatca cgaccgtaat attatcctca ccaccgcgat tgttcgcttc ttcaatcaac
     4201 gcatcacacg ccttcgaaag atcgtttctg ctgttgacca cgatatgctg aatatcttcc
     4261 gcgcgtaact tcccagacaa tccatcgcta caaagcaaca cgagatcgtc ctgtcgcaaa
     4321 cgaatcctgc ccgtcaccgg ctgcacctca ctctgcgcgc cgagtgcctg cagtatcacg
     4381 ttgcggaaca tgtgcgtctc agcttccgat tcgctgatct gtccgacgtc aaccagttgc
     4441 tgcacgagcg actggtcctt cgtcgcgagt cgaatctgat ctttacgaat cacataaccg
     4501 cgactgtcgc cgacctggac gaggtccagg ttatcaccgc ggaccgccgc gccggtgaac
     4561 gtggcgccca tgcccgcgca gcgcgagtct tcctgacttc tgaggtggat tgcagtgttg
     4621 gcgtaatgcg tcgcagactt gaggcactcg accagatcga gatcgggatc gcacacggca
     4681 tcgccctcgc cgacgagcat cttgcgcact gaatcgacag ccatgcgact cgcaacgtcg
     4741 ccggctagtg cgccgcccat gccgtcagag acgacgagga ccaagccttt gtcacccaga
     4801 ttgaagcgaa acaaattctc ggctggggga ttgccgtcgg cgccggtcca ggtttgttcg
     4861 gtcgacaggt cgagtacgag gaaattatct tcattgccac gtcgaactcg gccaacgtct
     4921 gatttggcgt ataactcaac aatcataaat ggagagctaa taggaagtcg ttaaataaag
     4981 gctgagaacc gggagattgt aacccatcct gttgatcctg aaaatcctgt tctaaagttg
     5041 tcccagggct tgatccaaat cggcaacaat atcgtccgta tcctcgatcc cgaccgagat
     5101 gcgcaccagc ccatccgtga tccctaatcg ctgccgcttt gcttcgtcaa ccgaagcgtg
     5161 cgtcatcgtt gccggatgcg aaatcagcgt ttccacgccg cccagacttt ccgcgagcgt
     5221 gcagagctta acagactcga gcaccttgcg cgcgttttcc agcgagccca catcaaatga
     5281 caccatgcca ccaaaaccgt gttgctggcg gcgcgccagg gcgtgctgcg ggtgcgacgc
     5341 ggagcccgga taataaacct ttttcacctt cggatgctct tccaaaaatg cggcgacggc
     5401 gcggccggtc ttgtcgtgct gttccatgcg cagggcgagg gtcttcgtgc cgcgcagaac
     5461 gagccacgag tcgaacggcg aaagaatcgc gcccgcgctg ttttgcacga agccgatcca
     5521 gtttgcatcc tcttcatcgt tcaggacgac gacgccgccg acgccgtccg aatgaccatt
     5581 gagatacttc gtggtcgagt ggacgacgat atcgacacca aactcgagcg gacgttgcag
     5641 gtacggcgac atgaaggtgt tgtcgcaaac gacgcgggca ccggcgcgat gcgcgatctc
     5701 actgacttcc ttcagatccg taaggatcat cacgggattg gtcggcgtct cgataaacac
     5761 catcttcgta tttggcttga tcgcggcctc gacgttcagt ggatcggatg tgtcgacgta
     5821 atcgaaagtc aggttgtagt tcgcgagtat gcgattgaag agacgtgcgg tgccgccgta
     5881 tgtgttatcg gagactatga cgtgttcgcc ggctttgacg agtcgcaggg ttgcgtcgat
     5941 cgcggccatg ccggaagcga atgcgaaacc gaaacgtccg ccttcgattg ccgcgatgtt
     6001 tcgttcgact gcggagcgcg tcgggttttg tgtgcgcgcg tactcgtagc ctttgttctt
     6061 tccgagtgcg tcctgcgcgt aggtcgatgt ttgatagatc ggaacggtga cggcgcctgt
     6121 tgtttcgtca ggttctgatc cggtgtgaat ggctgtagtt gcaaatccca tatattcagt
     6181 tactcgtcaa aaatcccctg gctgcgatat cgctcggcgc cgtcagggaa aactgtgacg
     6241 acgcgtttgc cagggccaag tcgttctgca attttgtacg acgcggcggc agcagcgccc
     6301 gacgaccagc cgccgagcag tccttccagt ttggcaatct tcttcagcgc cgcgcaaacc
     6361 tcgtcgtcct ggaccattag tatctcatca gccaacgagg catcgaagat cttcggaata
     6421 aaatcgcgct ggccgatgcc ttcaactttc tttggtccag gttggccacc gccgtaaatc
     6481 gatccttccg gctctacgag cacacacagc gcgttctgat tgtgttttcg cacggcgcgc
     6541 gcgacaccgc agaacgttcc ggaagttccc gcgccgatca cgaccgcatc gatctcgccg
     6601 ttcatttgtt cgatgatctc gcgtgcggtc gtgagttcat gaaaacacgg gttcgcatta
     6661 ttctcaaatt gctgcggcac gaaactgtcg tcaatcgttt ccgcgagctg gtgacaacgt
     6721 tcgatcgcgc cctgcatgcc cgcctcgttg gagacgtatt cgagctcgcc gcccagggct
     6781 tcgatcagtt tcatcttctc gactgcaaac ccgtgcggga cgcagagaat aacacgatag
     6841 tcacgcgcca ctcccacgag tgcgagcccg attcctgtat ttccggcggt cggctcgatg
     6901 atggttgagc ctggcttgag cagacccttt tcctcggcgt cgagaatcat ccccagcgcc
     6961 gcgcggtctt taacggatcc gcccgggttg agccattcga ttttcgcgta aatgccggcg
     7021 agtggtggtt gtgcgaaacg ggaaagatga agaaggggag tctggcctat gagatccaga
     7081 acactttctg cgacgccgcg gtgcacgttc cggatgttag cattagccac aaaaaggcac
     7141 aaaagtcgca aaatgatttt gtggtctttt gtgcccgttt gcggttcaag ctcagcaaga
     7201 atcgattgat aaatggatca ggcagtggta agttcgacgc gtggccataa actcagaaac
     7261 tctgg
//
LOCUS       DASSFQ010000132         6953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_495545, whole genome shotgun sequence.
ACCESSION   DASSFQ010000132 DASSFQ010000000
VERSION     DASSFQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495545"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VFH96_04560"
     CDS             complement(<1..1014)
                     /locus_tag="VFH96_04560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET6973270.1"
                     /translation="MKIAFVQSAIYLVAAWIVIRARSSNSTLLIVIAFAIVFRLSILF
                     APPYLSDDIYRYVWDGRVQAAGINPYRYIPAAPELAHLRDETIYPKINRKDWAHTIYP
                     PVAQIVFFLTTRISESVTWMKATMLLFELVTIWAVAQLLTLLNRPRQLLLMYAWHPLV
                     IWEFAGSGHVDAISISFIALALLAWQRKSDLGAGFMLGCATLVKLFPVVLAPVMLKRW
                     RIAPILATTISAGYLAYISVGPRAVFGSLPGYTQERGLLRGQQFYALSLAHRVFGFDL
                     PGLAYIIAVMLVMSAIGLWVLLKGRSEDHLKHAMVLATATTVLFAPHFSWYFCWLVLF
                     LCFT"
     gene            complement(1150..1473)
                     /locus_tag="VFH96_04565"
     CDS             complement(1150..1473)
                     /locus_tag="VFH96_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET6973271.1"
                     /translation="MTVKNLEVVKGSGNVFRDLEQKHPDLDQFKAILAAEIIKTLDRQ
                     KLSVRAAQGRTGIAAADFSRIRNADLRRFTVDRLVLIINRLGSRVEVRIKVRRTEKIE
                     QSLPA"
     gene            complement(1845..3224)
                     /locus_tag="VFH96_04570"
     CDS             complement(1845..3224)
                     /locus_tag="VFH96_04570"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6973272.1"
                     /translation="MPRKIPQILTVDDDAVTCELLCEVFAREGFGAVFANSGEAALEA
                     VAQQPPDLLLSDIRMKTPLDGLSLLEMVRREYPSIPVVLMTAFGSIETAIRAVKEGAF
                     DYISKPFNIDELVAIVRRALANGTGKQVRSELDDEAGSSGLIGRTPPMLEIYKMIARV
                     SDSRAAVLISGESGTGKELVARAIHNHGTRSTERFVAVNCGALTETLLESELFGHVKG
                     SFTGAIANKRGIFEQAGEGTVFLDEISETSAALQVKLLRVLQEREVVPVGGTDPIKVG
                     ARVIAASNSDLEKLSATGAFRGDLLYRLNVIQLHLPPLRQRREDIPLLVAHFLRKHAP
                     EPMASIALDQKAMHCLTTYSWPGNVRELENVIERAVTLNQGGVLAVDDLPARIRCQPS
                     HDTPPLTSDDLDALFSGLPNLDEIERRYILFVLDATGNNRKRTAEILGINRKTLYRMA
                     ARFEIEFAH"
     gene            complement(3214..4791)
                     /locus_tag="VFH96_04575"
     CDS             complement(3214..4791)
                     /locus_tag="VFH96_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973273.1"
                     /translation="MRLRVNLQQKILLLVAGSMSLILLASSYLHSGRIRSLIEKDHYD
                     NALNQTTALGNRIAQYDYFSNLEDLEQEMQLVVSSRPDFRQIDVYENQETVPRLIATT
                     ASGTANLLSAWDDSGPATTGATSSQINRNDGVYWLITTPISNGQHTGFIKALVVKSYH
                     RNLVNSLHREYNLVLFGAVIASVLLLYLLFVYFFRKPVKDIVLTMTEARNGNLSARAL
                     VHRDDELGEIAGNFNQMMDDIASRSREREELLSQIGSLNNQLVKKVHLATTELRAANG
                     NLIRTQQRLSHAERMAAIGQVTASLAHEIGTPLNAINGHLQLLARNHPSCGDTQRRLN
                     IINAQLASIVHSVKSLLERTHRRPIDPQPTDINLIVEELLLLVAPIFESHNIRVSHQL
                     DEGLPPVLADRESLHQVFLNLVNNSLEAMPEGGEMKFVTNYLAASKVIEVRLIDTGTG
                     ICDEAKNHLFEPMWTTKKSGSGLGLAIAREIVLEHGGRIECTLEVEKGAEFRVVLPAV
                     EVKYVAPEFAEVSLDAA"
     gene            complement(4923..6272)
                     /locus_tag="VFH96_04580"
     CDS             complement(4923..6272)
                     /locus_tag="VFH96_04580"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6973274.1"
                     /translation="MKHILVVDDDRDSCELLREIFCAEGWHVDTALSPTQAFSVAEKE
                     KIDLVVSDINLEAHQSGLDLLRDLRAQCPVILVTGFGTLDSAVEAAREGAWDFISKPF
                     KVQEVVAIARRALEQDRNSSGAERRAEQLSGPYEQGGLLGRSPVMIGLYKEIARVAPT
                     RSTILIAGESGAGKELVARSIHQHSTRSGGSFVAINCGALTETLLEAELFGHVRGSFT
                     GAVGDRKGLWEEATGGTLFLDEVGETSPAFQVKLLRALQEGEIRRVGASRTTQVDARI
                     LAATNRNLEQEVKAGKFREDLYYRLSVVTLRVPALKERRTDIPLLAERFLAGVLDREG
                     HKQLTLSEETIRTLVAYNWPGNVRELESAIEYAVLHARGHAIAPEDLPEKLQSAQVRT
                     AARAPLSALFEDLPALDELERRYLLYILEIAGGNRTRAAEILGIDRRTLYRMIERYE"
     gene            complement(6269..>6953)
                     /locus_tag="VFH96_04585"
     CDS             complement(6269..>6953)
                     /locus_tag="VFH96_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6973275.1"
                     /translation="GTPLNLISGHVQLLKSDLDRDPHDAAARIRTISAQIERIERIVR
                     RMLDKTRFETELAPLDLNSMLRKLCDAMAPALDKRNIRLVETFADDLPLIAGSSDRLQ
                     QLFLNLINNSLDAMPEGGEIRVCTACEGKFGKAQRVTVDFIDTGFGMTPEVMSHIFDP
                     LYTTKDRGHGTGLGLVIVSQIVSEHGGRVDVESEIGKGTRFRLTFAAIPTDVAAVVEE
                     PELELGVSR"
BASE COUNT         1579 a         1986 c         1872 g         1516 t
ORIGIN      
        1 tgtgaagcac aggaagagaa ccagccagca gaaataccac gagaaatgcg gtgcgaacag
       61 caccgtggtg gccgttgcta acaccattgc gtgtttcaga tggtcctcac tacgtccttt
      121 taagagcacc cagagcccaa tcgcgctcat caccagcatc accgcgatta tgtaagcaag
      181 ccctggcaga tcgaatccaa atactctgtg cgctaagctc aacgcataga attgttggcc
      241 acgaagtaat cctctttcct gcgtgtatcc gggcaaagat ccgaaaacgg ctcttggacc
      301 aacgctgata taggcgagat aaccggcgct gatcgtcgtc gccaggattg gtgcgatgcg
      361 ccagcgtttg agcattaccg gagctaaaac gacagggaac agtttgacca gtgttgcgca
      421 accgagcatg aaacctgcgc cgagatcaga ttttctctgc cacgcgagca acgccaaagc
      481 gataaaagag atggaaatcg cgtcgacgtg tccgctaccg gcaaactccc agatgacgag
      541 tggatgccat gcgtacatga ggagaagttg tcgcgggcgg ttgagtaaag tgagcaactg
      601 cgccaccgcc cagattgtta ccaactcaaa caagagcatc gttgccttca tccaggtgac
      661 actctcgctg attctggttg tgaggaagaa aacgatttga gcgacgggcg gatagatcgt
      721 gtgagcccag tcttttcggt ttatcttcgg atagatagtc tcgtcgcgca ggtgggccag
      781 ctcgggcgcc gcaggtatgt aacgatacgg attaataccc gcagcctgca cccgtccgtc
      841 ccacacgtat cgatagatat cgtcagatag gtatggcggc gcgaagagta tgctcagccg
      901 aaatacgatt gcgaaggcga tgacgatgag aagagtggaa ttcgaagagc gagcgcgaat
      961 aacgatccat gccgcgacca ggtagatcgc cgattgaacg aacgcgatct tcatatagaa
     1021 ccggatgcca ctaacaccgc tggcattcag accagcccgg taaagcacaa ccgaaacaca
     1081 gcccagaagg ataagaacaa agccgctttt aatgtcgagg tccaggtggg gtcgcatcgc
     1141 tggaatgtgt tacgcaggca ggctctgttc aattttttca gtacgtcgca ctttaatcct
     1201 cacttccacc cgcgacccaa gccgattgat gattaacacc aaacgatcga cagtaaatcg
     1261 cctaaggtct gcattacgga tgcgggagaa atctgccgcg gcaatgccgg tacggccttg
     1321 cgctgcccga acactcaact tctgccgatc aagcgtctta atgatttcgg cggctagaat
     1381 tgccttgaac tgatcgagat ctggatgttt ttgttcaagg tcacgaaaaa catttccgct
     1441 tccctttacg acctccaaat ttttaacggt cattgcacca tctccttcaa cctctttaag
     1501 cggcgcatgt tgtcataatt gccaacatca ggtcaatcgc cgagcttgaa gacttgtcgc
     1561 ttttgaacgt tggcccgaag tggcgagtgc acgcagagcc cgcgaagcgg gcgaaagcat
     1621 aaagcctggg gcgcaagccc caggatcacg aaataaaaaa tatgggcaga gcccgtgata
     1681 gcgggcgata gcgttttagg ctcatacgct gtcgcccgct ttcgcgggct caataatgtt
     1741 ttgcgatcat gatcctgggg cttgcgcccc aggctttatg ctttcgcccg ctttgcgggc
     1801 ttgcaacccc aaggcttagg ctctttgatt cgcggactgt ttttctagtg tgcgaactcg
     1861 atctcaaacc gggcggccat gcggtagagg gttttgcggt tgatgccgag gatttcggcg
     1921 gtgcgcttgc ggttgttgcc ggtggcgtcg aggacgaaga ggatgtagcg gcgctcgatc
     1981 tcgtcgagat ttggtagccc tgaaaagagc gcgtcgagat cgtctgaggt aagtggtggt
     2041 gtatcgtgtg atggttggca acgaatgcgc gccggcagat cgtccacggc caaaacacca
     2101 ccctggttca gcgttaccgc ccgctcgatc acgttttcca attcacgcac gttgccgggc
     2161 caggagtagg tagtcaggca atgcatggcc ttctggtcta aggcaattga agccattggt
     2221 tcaggcgcgt gttttcgcag gaagtgcgcg accaatagag gaatgtcttc gcgtcgctgt
     2281 cgcaatggtg gcaggtgcag ctgaataacg ttcagccggt atagcaaatc gcctctaaaa
     2341 gcgccggtag cggaaagctt ctccagatca gaattacttg ctgcaatcac acgcgccccg
     2401 actttgatcg ggtccgttcc accaaccggc accacctcgc gctcctgtaa aacacgcagt
     2461 aacttaacct gcaacgcggc actggtttcg ctaatctcgt cgagaaacac cgtgccttca
     2521 cccgcctgct cgaagatccc gcgcttgttt gcaatcgcgc cggtaaatga acccttaacg
     2581 tgtccaaaca actccgactc gagtaacgtt tccgtcaacg cgccgcaatt cactgccaca
     2641 aaacgttctg tcgatcgtgt gccgtggttg tgaatcgcgc gcgcaacgag ctctttgcca
     2701 gtcccggatt caccactgat cagcaccgcg gcgcgcgagt cggaaacacg agcgatcatc
     2761 ttgtagatct caagcatcgg aggcgtgcgt ccgatcagac ccgagctacc cgcttcgtca
     2821 tcgagctcag atctcacttg cttccccgtt ccatttgcga gtgcacgtcg cacgatcgcc
     2881 accagctcgt cgatattgaa cggcttgctg atgtaatcaa acgcgccttc tttaactgct
     2941 ctgatagccg tctcgatcga gccaaacgcc gtcatcaaca cgacgggaat cgacggatac
     3001 tcgcgacgaa ccatctccag caacgacagc ccatcgagcg gagtcttcat gcgaatatca
     3061 ctgagcagca gatccggtgg ttgctgagcc acggcctcga gcgctgcctc gccgctgttc
     3121 gcgaaaacag caccaaaacc ctcgcgagca aaaacttcgc acagtagctc acacgtgacc
     3181 gcatcgtcgt cgacggtcaa aatctgcggg atcttacgcg gcatctaaac tcacctcagc
     3241 aaattccggc gccacatact taacctccac cgccggcagg accactcgaa actcggcgcc
     3301 cttctcaact tccaacgtgc actcgatccg gccaccgtgt tcgagaacga tctcgcgggc
     3361 aattgcgagt ccgaggccgc tgccggattt tttggtggtc cacatcggct cgaacaaatg
     3421 attctttgct tcgtcgcaga tgcctgtgcc ggtgtcgatc aaacgcactt cgatcacttt
     3481 cgacgccgcg aggtagttcg taacaaactt catctcacca ccttccggca tcgcttcgag
     3541 actgttattc acgagattga gaaacacctg gtgcagactt tcgcgatcgg caagcaccgg
     3601 cggcaaaccc tcgtcgagct ggtgcgacac acgaatgttg tgcgactcaa aaatcggggc
     3661 gaccagcagc aacagctctt caacgatcag attgatatcg gttggttgtg gatcgatcgg
     3721 gcggcggtgc gtgcgctcga gtaaactctt caccgagtgc acgattgaag cgagttgtgc
     3781 gttaatgata ttcagtcgtc gttgcgtgtc accgcagctc ggatgattac gcgccagcag
     3841 ttgcaggtgt ccgttgatgg cgtttagcgg tgtgccgatc tcatgtgcga gcgatgcagt
     3901 tacctggccg atggcagcca tcctttccgc atgcgagaga cgctgttgcg tacggatcaa
     3961 atttccattc gccgcgcgca attcagtagt agcgaggtga accttcttca ccaactggtt
     4021 attcaaactg ccaatctggc tcaacaactc ttcgcgctct cgcgatctgg acgcaatgtc
     4081 gtccatcatc tgattgaagt tacccgcaat ctctcccagc tcatcatcac gatgcaccaa
     4141 cgcgcgcgcc gaaagattac cgttacgagc ttcggtcatc gttaagacga tgtctttcac
     4201 aggtttgcga aaaaagtaga caaagaggag atacagcagc agcaccgacg cgatcactgc
     4261 gccaaacaac acgaggttgt actcgcgatg aagactattg accagattgc gatgataact
     4321 cttgacgacc agcgccttga tgaaaccggt gtgttgaccg ttgctgatcg gagtcgtgat
     4381 cagccagtac actccatcgt tgcgattgat ctgagatgac gtagcgccgg ttgtggccgg
     4441 accgctgtcg tcccaagcgg agagaagatt cgcggtgcct gaagcagttg tggcgattaa
     4501 ccgcgggaca gtctcctgat tctcgtaaac gtcgatctgt ctgaagtcgg gccgcgagct
     4561 gacaaccagt tgcatctcct gttcgagatc ttcgagattt gagaagtagt cgtactgcgc
     4621 gatccggtta ccgagcgcgg tcgtttggtt gagggcgttg tcgtaatggt ccttctcgat
     4681 cagcgatcgg attctgccgc tgtggaggta cgacgaagct agcaggatca acgacatcga
     4741 accagccaca agcagcagaa tcttttgctg taggtttacg cgtaaacgca tcgcgattca
     4801 cgcaccgcaa ttcttaagcc gcggatacac gcggataaac gcagatctgc gttcatctgc
     4861 gtaaatccgc ggctaaaacg tttgtggcta tttaggacca ggaggcaaaa gggccacagt
     4921 ggttattcgt agcgctcgat catgcggtag agggtgcggc gatcgatgcc gaggatttcg
     4981 gcggcacggg tgcgattgcc gcccgcgatc tcgaggatgt agagtaggta gcggcgttcg
     5041 agctcatcga gcgcgggtag atcttcgaag agtgctgata acggtgcgcg cgcggcagtg
     5101 cgcacctgcg ctgactgcag tttttccggc aggtcttccg gcgcgatagc gtgaccacgc
     5161 gcgtgcagca cggcgtattc gatcgccgac tcgagctcgc gcacattgcc gggccagttg
     5221 taagcgacga gcgttctgat ggtctcttcg gagagagtta actgcttgtg tccttctcga
     5281 tcgagcaccc ccgcgaggaa acgctcagcc aacagcggaa tgtctgtgcg gcgttccttc
     5341 agtgccggca cgcgcaaggt caccacactg agccggtaat aaagatcctc gcgaaacttc
     5401 ccagccttca cttcctgttc gagattacga tttgtggctg cgagaatacg tgcatctacc
     5461 tgcgtggtgc gcgaggcgcc aacccgcctg atctcacctt cctgcaacgc acgcaacagt
     5521 tttacctgaa acgccggact cgtctcacca acctcatcga gaaaaagcgt tccacctgta
     5581 gcttcttccc aaagcccttt acgatctccg acagcgcccg tgaacgatcc gcggacgtga
     5641 ccaaacaact ctgcttccaa aagcgtctcg gtgagcgcgc cacagtttat ggcgacgaac
     5701 gatccgccgg aacgtgtgct gtgttgatga atcgatcgtg ccacgagctc ttttcctgcg
     5761 cctgattccc cggcgatcag aatcgttgag cgagtcggcg ccacgcgcgc gatctctttg
     5821 tagagtccga tcatcacggg agagcgcccg agcaatccgc cctgctcgta cggcccggaa
     5881 agctgctcgg cgcgccgctc agcaccgctg ctgtttcggt cctgctcgag ggccctgcgt
     5941 gcaatcgcca caacttcctg gaccttgaac ggtttggaga tgaaatccca ggcaccctcc
     6001 cgcgccgcct cgacggcgga gtcgagcgtg ccgaaccccg tcaccaaaat cacgggacac
     6061 tgtgcacgca aatctcgcaa cagatccaat cctgattgat gcgcttcgag attgatgtcg
     6121 ctgacgacca gatcgatctt ctctttctcc gccacactaa aagcctgcgt cggactgagc
     6181 gcagtatcga cgtgccagcc ttccgcgcag aagatctcgc gcagcagctc gcatgaatca
     6241 cgatcgtcat cgaccacaag aatatgtttc atctacttac acctaactcg agctcaggct
     6301 cttcgacaac ggccgccacg tcagtcggta tcgctgcgaa ggtcagacga aagcgagtgc
     6361 ctttcccgat ctcactttca acgtcgactc gccccccatg ctcagaaacg atttgactaa
     6421 cgatcacgag accgagcccg gtgccatgac cgcgatcttt cgtcgtgtaa agcggatcaa
     6481 aaatgtgact catcacttcg ggcgtcattc cgaatccggt gtcgatgaaa tcaaccgtaa
     6541 cccgttgcgc tttcccaaac ttgccctcgc aggcagtgca aacccgaatc tcgccgccct
     6601 caggcatcgc atcaagactg ttgttgataa ggttcaaaaa cagttgctgc aaacgatctg
     6661 acgaaccggc gatcagcggc agatcgtcag cgaatgtttc gacgagacgg atgttgcgtt
     6721 tgtccaacgc aggcgccatc gcgtcgcaga gtttgcgcaa catcgaattc agatcgagtg
     6781 gcgcgagctc agtttcgaat ctcgtcttgt cgagcatgcg acgcacgatt cgttcgatgc
     6841 gctcgatctg cgcgctgata gttcggatgc gcgcggccgc atcgtgcgga tcgcgatcga
     6901 gatccgattt aagcaactgg acgtgaccgc tgatcaggtt gagcggcgtg ccg
//
LOCUS       DASSFQ010000133         8378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1541912, whole genome shotgun sequence.
ACCESSION   DASSFQ010000133 DASSFQ010000000
VERSION     DASSFQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541912"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..2043
                     /locus_tag="VFH96_04590"
     CDS             184..2043
                     /locus_tag="VFH96_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6973276.1"
                     /translation="MLNERELKLIVDTIPALAWSGRPDGSAEFFNQHFLDYVGLSAEQ
                     ASDWGWTTAVHPEDREGLVLTWQAIMASEQLGEGEARLRRHDGEYRWFLFRANPVRDE
                     SGRVVRWYGINIDITERQLALERLQANQDLLDLAQKSAGAMAFDWYPQQEINYLSPEQ
                     EILYGLAPGTFDGRFKTWKGMIYAPDWPTVVDAIKQAKRRGKVTVEYRVVWPDGTLHW
                     LSTNGRMFFDDAGEPVRVVGFTSDVTRQKLVEEELRRSEAFLAQAQRLSRTGSFSWRI
                     AGDEITWSEQLYCIYELDPGTTITLDVIRTRVHPEDLTLYEKMVEQARNGSEDFEWQY
                     RLLMPDRSIKYLHAVAHLIRDGNGDLEYIAAVQDVTARRSSEEALDKARSELAHVARV
                     MSLGALTASIAHEVNQPLSGIITNASTCLKMLDADFPNVDGARETAKRTIRDGHRAAD
                     VITRLRALFSKTEAATELVDLNEATREVIALSRSELERNRVITRTELADQLPLVTGDR
                     VQLQQVILNLLRNGSDAMSEVHDRPRDLLFKTEVDEADCVRLSVRDAGTGFESQTVEQ
                     LFRAFYTTKADGMGIGLSVSRSIIENYGGRLWATPNDGPGVTFAFSIPCTKNF"
     gene            complement(2114..3316)
                     /locus_tag="VFH96_04595"
     CDS             complement(2114..3316)
                     /locus_tag="VFH96_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6973277.1"
                     /translation="MSYAPQPEADKSAIRPFQFNFTDAELADLRKRVNATKWPERELV
                     NDESQGVQLATMQKLARYWGTDYNWRKGEAKLKAVPHFVTEIDGLDIHFIHVRSKHEN
                     ALPMIITHGWPGSVIEQLKIIDPLTNPTAHGGTAADAFDVVIPSLPGYGFSGKPTAPG
                     WDPIRVARAWVVLMQRLGYTKFVAQGGDWGNAVTEQMALLTPPGLVGIHTNMPATVPG
                     EIEKLLPAGPPPAGLSPDEKDAWDQLSYFYQHGLGYAQEMANRPQTLFALTDSPTGLA
                     AWMIDHDIASYKLIARVFDGQSEGLTRDDILDNITFYWLTNTAVSSARLYWESKLAFF
                     APKNVQVPTAISVFADEIYAAPKSWTEKAYPKLIFYNRHDKGGHFAAWEQPASLTADL
                     RTAFKPLR"
     gene            3564..3974
                     /locus_tag="VFH96_04600"
     CDS             3564..3974
                     /locus_tag="VFH96_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973278.1"
                     /translation="MLRSIDNVLRGILMVARPLISVVDDDESIRESLPDLLREYGFEA
                     RVFTSAEEFLASEAVGATNCLLLDVAMPGMTGPDLQRELKLREQSIPIVFITAHRDAN
                     VRPRLIAQGAVECLFKPFEPTDLLEALNTALRVS"
     gene            3995..4657
                     /locus_tag="VFH96_04605"
     CDS             3995..4657
                     /locus_tag="VFH96_04605"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973279.1"
                     /translation="MTPAVKSQPQPIVFVVDDDISVRESLELLIQFAGWQPEIFASAE
                     DFLAHPRTATPSCLVLDVSLPDLNGLELQKLIASERTDMPIIFITGHGDVPMSVQAMK
                     AGAVEFLIKPFDDEVLLSAIRHAIERSAAVLEDQAEITTLRSSCESLTPREQEIMLLV
                     VAGMLNKQIGVRLGISEITVKAHRGKVMQKMNADSLADLVKTAVRLGLTPAKNPWHKN
                     DR"
     gene            4672..5295
                     /locus_tag="VFH96_04610"
     CDS             4672..5295
                     /locus_tag="VFH96_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6973280.1"
                     /translation="MAEVDRSRRKFLKTSGTTAAAGVISAYLPAHATAAESTPESEAT
                     RSVIEGAVPITLRINGKDHKLNIDPRTTLLDCIRETIVLTGTKKGCDHGQCGACTVHV
                     NGKRVLSCLNLALMHDGNEITTIEGLGTPDAMHPMQKAFVDCDGYQCGYCTSGQIMSA
                     VALLKEPCGPDDAAVKELMSGNICRCGAYANIVQAIQQVRNSASDRA"
     gene            5308..6309
                     /locus_tag="VFH96_04615"
     CDS             5308..6309
                     /locus_tag="VFH96_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET6973281.1"
                     /translation="MHTFEFIRPPDAAAAIAAAAKSKTAQQGADVRFMGGGTTLIDLM
                     KLSVETPDRVLDINRLPLDKIEVMPDGGLKIGATARNSDLAYHAKVQSDYSVLSQAIL
                     LGASAQIRNMATVAGNLLQRTRCVYFRDTAMPCNKREPGTGCPAITGHNRTLAIFGAS
                     EHCIATNPSDMCIALAALEATVHIQGPKGTRAVAFGDFHVLPGNTPQRETVLEPGDLI
                     THVTLPPPVAGSKQHYLKLRDRASYEFALASTAVVLTIAGGKITRARIALGGVGTKPW
                     RSPEAEAALTGKPANEATFRQAAEAALQGAKPQSENRFKIELAKRCITHALRTAAAA"
     gene            6324..>8378
                     /locus_tag="VFH96_04620"
     CDS             6324..>8378
                     /locus_tag="VFH96_04620"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6973282.1"
                     /translation="MATTQPSSATTPLGRDTLRIDGPRKVTGMAQYTSDFNFPGMLYG
                     VPVEATIANGKLVALDTAAAEKMPGVRKIFHRANIGKIFRSTPAPGFERVCLERRPPF
                     EDDEIRYYGQYIALVVADTFETAKAAADAVRATYTKEKPNVETHLEAGNDPDVVFTGY
                     GPVPRLQSQRGDPDAAFASAPVKLDETYSTPPETHNPLELHATTAMWDGDILTLYDST
                     QGVVNMRSVMAQMFGLPKENVRVIAKFLGSGFGGKLYPWTHVSLAAAAARQLGKPVKL
                     VVSRKMMFQTVGHRARTQQRMRLGATREGKLLSFQHDYVYHLSMIDHYHEDCGEASAF
                     HYSVPNLRVHWGRARRNIGATADMRGPGAVPGLYATESAMNEMAFRLKIDPVQFRLLN
                     EPKLDESNNLQFSSRHYVECLNLGVEKFGWSKRNPAVGSMKRDGLTLGWGMAGAAWIA
                     GRFAAAASLQLRDDGTVRIACGTQDIGTGTYTILAQLASEKTGVSLDKIEVELGDSAL
                     PDGPISGGSLATSSVVPAVFQAADQAITSLMILAVTTPGSPFLGRKPEDLALEGGRIF
                     VKAEGVGKGVAFGEVLRKANARLVAGSGSADQTVFAANAKFSMHSFGCHFAEVTWQPE
                     TARLRVSRVVTVIDAGRILNPLTGRNQIQGAVVMGIGMALFEETAYDPQNGAPINSNL
                     ADY"
BASE COUNT         1853 a         2312 c         2448 g         1765 t
ORIGIN      
        1 gagccgaata ggatcgatca gaccacttag tgagctttga tcaatgatcg accaccttga
       61 agcagccatt tatcaacctt aatcagagtt tcctttagtt tgtcgcgaca ttccgacaag
      121 ctaaagctta tcggacatca agcttgactt ggcttttacg cggcatggag gcattctcac
      181 gccatgctca acgaacgtga gctcaaactc attgtcgaca cgatccctgc gctggcgtgg
      241 tctggtcgtc cggatggcag cgcggagttc ttcaaccagc atttcctcga ttacgtcgga
      301 ctctccgccg agcaagcgag cgactggggt tggaccaccg cggttcatcc ggaggatcgc
      361 gagggtttgg tgctcacttg gcaggccatc atggcgtcgg aacagttagg tgagggtgaa
      421 gcacgcctgc gccgtcatga cggcgagtac cgctggttcc tctttcgcgc caatcccgtg
      481 cgcgacgagt caggacgggt cgtcagatgg tatggaataa acatcgacat caccgaaaga
      541 cagcttgcac tcgagcgctt gcaagcgaat caggatttgc tcgacctcgc gcaaaagtct
      601 gccggtgcga tggcgtttga ttggtatccg caacaggaga tcaactacct gtcgccggaa
      661 caggaaattt tatacggcct cgcgccgggc actttcgacg gaagattcaa gacgtggaag
      721 ggaatgatct atgcgcccga ttggcccacc gttgtcgatg cgattaagca ggctaagcga
      781 agaggcaagg taactgtcga gtatcgcgtc gtgtggcctg acggaacgct gcattggctt
      841 tcgacaaacg gtcgcatgtt ctttgacgat gccggcgagc ccgtgcgagt ggtgggcttc
      901 acttccgatg tcacgcgaca aaagttagtc gaagaagaac tgcggcgtag cgaggcattt
      961 ctggcgcaag cacaacgact cagtcgcacc ggcagttttt cgtggcgcat cgccggggat
     1021 gaaatcacgt ggtccgagca gctctattgc atttatgaac tcgatccggg aacgacgatc
     1081 acactcgatg tgatccgcac ccgcgtacat cccgaagatc tcaccttgta cgagaaaatg
     1141 gtcgaacagg cacgaaacgg cagcgaggat tttgagtggc agtaccgttt gctcatgccc
     1201 gatcgatcga tcaaatacct gcatgccgtt gcgcatctga tacgcgatgg gaatggtgac
     1261 ctggagtaca ttgcggcagt tcaggacgtt accgcgcgcc ggtcttccga agaggcgttg
     1321 gacaaagcgc gctcagagct cgcgcatgtg gcgcgggtaa tgagtctcgg cgctttgacg
     1381 gcttcgatcg cgcatgaagt gaatcaacca ttgtcgggca tcatcacgaa tgcgagtacg
     1441 tgtctgaaga tgttggacgc tgatttcccc aatgtcgacg gcgcacggga aactgcaaaa
     1501 cgcaccattc gtgacggcca ccgtgctgct gatgtaatca cacggctgcg cgctctcttt
     1561 agtaagacgg aggcagcgac agaattggtg gacctgaatg aggcgactcg cgaagtgatt
     1621 gcactgtcgc ggagcgagct cgaacggaac cgcgtcatta ctcgaactga actagccgat
     1681 caactgccgc tcgtgacggg cgatcgggtg cagctacagc aagtgattct caatctactg
     1741 cgtaacggct ctgacgcgat gagtgaagtc catgatcgcc cgcgcgattt gttgttcaaa
     1801 actgaagtgg acgaagctga ttgtgtgcgt ttgagtgtgc gagatgctgg cacgggtttc
     1861 gagtcacaaa ctgtcgagca gctatttcgg gccttctaca caaccaaagc tgacggcatg
     1921 ggaatcggtc tctccgtcag ccgctcgatc atcgagaact atggtggtcg tctatgggcc
     1981 actccgaatg acggtccggg tgtgacgttt gcgttttcga tcccgtgcac gaagaatttt
     2041 tagccacaaa aaggcacaag gcacataaag agaacaagtc tttttatgtg ccttttgtgc
     2101 ctttttgtgg ctattaccgc agaggcttga acgctgtgcg caggtcagcg gtcagagagg
     2161 ccggctgttc ccaggctgcg aagtgaccgc ctttgtcgtg cctgttgtag aagatgagtt
     2221 tcggataggc cttctcggtc caactcttcg gcgcggcgta gatctcatcc gcaaaaacgc
     2281 tgatcgccgt cggtacctgc acattcttcg gcgcaaagaa tgccagcttg ctttcccagt
     2341 agagacgagc ggaagagacg gccgtgttcg tcaaccagta gaacgtgatg ttgtcgagga
     2401 tgtcgtctcg tgtcagaccc tcactctgac catcgaagac acgcgcgatc agcttgtaac
     2461 tcgcgatgtc gtggtcgatc atccaggcgg ccaaaccggt cggcgaatcc gtcaacgcga
     2521 acaaggtctg cggacggttc gccatctcct gcgcgtagcc gaggccgtgc tggtaaaagt
     2581 agctgagctg atcccaggcg tctttctcat ctggtgaaag accggccggc ggtggaccag
     2641 cgggaagcag tttctcgatc tcgcccggta cagtcgccgg catgttggta tggatcccga
     2701 ccaatcccgg cggcgtgagt aatgccatct gctccgtgac tgcattgccc caatcaccgc
     2761 cctgtgccac aaacttcgta tatccgagac gctgcatcaa caccacccac gcgcgcgcga
     2821 cgcgaatcgg atcccaacca ggcgcagtcg gcttgcctga aaaaccgtaa ccaggcaagg
     2881 aaggaatcac gacatcgaac gcatctgccg cagtgccgcc atgcgccgtt ggattggtta
     2941 acggatcgat aattttgagc tgctcgataa ccgagccggg ccagccgtgc gtgatgatca
     3001 ttggcagcgc attctcatgt ttcgaacgaa cgtgaatgaa atgaatatcg agcccatcga
     3061 tctcagtgac gaagtgcggc accgctttca gtttcgcctc accctttcgc cagttgtagt
     3121 cagtgcccca gtagcgcgcg agcttctgca tcgtcgcgag ctgcactccc tgtgactcat
     3181 cgttaaccag ttctcgctcg ggccacttgg ttgcgttgac gcgtttacgc aagtcggcta
     3241 attcggcatc ggtaaaattg aactgaaacg gacgaatagc actcttgtcg gcttccggtt
     3301 gtggggcgta actcatcgag atctcctttt gattagtgct tgaattttta aatgcaatgt
     3361 tccttggact gaacgcgaat gccattccca gtagcgaccc ggcttttaga aaatggcggc
     3421 gcgtagtttc tgatttgttg gtcatatttg tgcggcagta caccacgagt gtgaaagtga
     3481 atctattaga caaaggtatc gatcgatacc aaagtatgat ggacatcccg cctcgatacg
     3541 gggcattctc cgtaaatact ctgatgttga gatcgattga caatgtgttg cgaggcatac
     3601 tgatggttgc acgtcccctc atatcagttg ttgatgatga cgaatccata cgtgagtcgc
     3661 ttcccgatct cctcagggaa tatggttttg aagcacgagt gtttacgtca gccgaagagt
     3721 tcctggcttc tgaagcagtc ggagctacga actgtctgtt gcttgatgtg gcgatgccgg
     3781 gtatgactgg tccagacttg cagcgagagc taaaacttcg tgagcaatcg atcccgattg
     3841 tctttatcac ggcgcatcgc gatgcgaatg tgcggcctcg tttgatcgca caaggcgccg
     3901 ttgagtgttt gttcaagcca ttcgagccga cggatctcct ggaagcactt aacacagcgc
     3961 ttcgagtgag ttgaatggca gcgttttcgt cgatatgacg ccagcagtta agtcgcagcc
     4021 gcagccgatc gtctttgtcg tagacgacga tatctctgtg cgcgaatcgc tcgagctgct
     4081 gatccaattc gccggctggc aaccggagat atttgcgtcg gcagaagact ttcttgctca
     4141 tccgcgaacg gccacaccga gttgtctggt gctcgacgtc tcgctgccag atcttaatgg
     4201 tctggagctg cagaagttga tcgcatcaga gcgaaccgac atgcccatca tcttcattac
     4261 gggccatggc gacgttccca tgtcggtcca ggcgatgaag gcgggagcgg ttgagttcct
     4321 aatcaagcca tttgatgacg aggtgctgtt gagtgcgatt cgacacgcga tcgaacgaag
     4381 cgccgcggtg ctcgaagatc aggctgaaat cacgaccctg cggagcagtt gcgaatcact
     4441 gacgccgcgt gagcaggaaa tcatgctgtt agtggtagcc ggaatgttga ataagcagat
     4501 tggagtgaga ctcggaatca gtgaaatcac ggtaaaggcc caccgcggca aagtgatgca
     4561 gaagatgaat gctgattcgc tcgccgacct ggtaaagaca gcagtgagac tcggtctcac
     4621 acccgctaaa aatccatggc acaaaaatga tcgttaaaga gaggaagaat catggctgaa
     4681 gtcgatcggt ctcgacgcaa gtttctaaag acgagcggaa ccaccgcggc cgctggtgtg
     4741 atctcggctt acttacctgc acacgcgact gctgccgaat caacgccgga aagtgaagct
     4801 actcgctctg ttatcgaagg cgcggttccg atcacgctgc gtatcaatgg caaggatcac
     4861 aaattgaaca tcgatccgcg cacgacgctg cttgattgca tacgcgaaac gattgtgctc
     4921 accggaacaa agaagggttg cgatcacggg caatgcggcg cgtgtacggt ccatgtcaat
     4981 ggtaagcgcg tgctctcttg tctgaatctc gcactcatgc acgatggtaa cgagatcacg
     5041 acgattgagg gactcggtac gccggacgca atgcacccaa tgcaaaaggc tttcgtggac
     5101 tgtgacggtt atcaatgcgg ctactgcacg tcaggacaaa tcatgtcggc agtggcactg
     5161 ctcaaggaac cctgtggtcc ggacgacgcg gcagtgaagg aactgatgag cggcaacatc
     5221 tgccgctgcg gcgcgtacgc gaatatcgtc caggcaattc aacaggttcg aaacagcgct
     5281 tcagaccgag cgtaaggaga aatccccatg cataccttcg aattcattcg accaccagat
     5341 gccgcggctg ccatcgccgc cgccgcaaaa tcgaagacgg cgcaacaggg cgcggacgtg
     5401 cgcttcatgg gtggcggcac cacgctgatc gatcttatga agctgagtgt cgagacgccc
     5461 gatcgagtac tcgacatcaa tcgtctgccg ctcgacaaga tcgaagtcat gccggatggc
     5521 ggattgaaga tcggtgccac tgcgcgcaac tccgatctcg cgtatcacgc aaaagtgcag
     5581 agcgattact cggtgttgtc gcaagcgatc ctgctaggtg cttcggcgca gatccgcaat
     5641 atggctactg ttgccgggaa cctgttgcaa cgcacacgct gcgtctactt ccgcgacacc
     5701 gcaatgccgt gcaacaaacg cgagcccggc actggatgtc cggcgattac gggccacaat
     5761 cgcactctcg ccatcttcgg cgcgagcgag cattgcatcg cgacgaatcc ctcagacatg
     5821 tgcattgctt tggcggcctt ggaagcgacg gtccacattc aggggccgaa aggtacacgc
     5881 gccgtcgcgt tcggcgattt tcatgttctg cccggcaaca caccgcagcg cgagaccgtg
     5941 ctcgagccgg gcgatctcat cactcacgtc acactcccgc cgccagtcgc cggcagcaag
     6001 cagcactatt tgaagttacg cgatcgcgcg tcttacgaat tcgctttggc ctcgacggca
     6061 gtagtgctca cgatcgcggg cggcaagatc acgcgcgcgc gtatcgcact cggcggagtg
     6121 ggcacaaaac cctggcgctc gcccgaagcc gaagccgcac taaccggcaa gcccgcgaac
     6181 gaggcgactt tccgccaggc tgccgaagcg gcgttgcagg gcgcaaagcc gcagagtgaa
     6241 aaccgattca agatcgagct ggcgaaacgt tgcatcacgc acgcgcttcg cacggcagcc
     6301 gcagcctgat ttgaggagca accatggcaa ctacacaacc atcatctgcg actacaccgc
     6361 tcggacgcga taccctccgc atcgatgggc cacgcaaagt caccggaatg gcccagtaca
     6421 cttccgactt caacttcccc ggcatgcttt acggcgttcc cgtggaggcg acgatcgcga
     6481 atggcaaatt ggtcgcgctc gatacggcgg cggctgagaa gatgccgggc gtacgaaaaa
     6541 tttttcatcg cgctaacatc ggcaagatct ttcgttccac tccggcgccg ggcttcgagc
     6601 gcgtctgcct ggagcggcgg cctccgttcg aagacgacga gatccggtac tacggccagt
     6661 acatcgctct cgtcgtggcg gacactttcg aaaccgcgaa agcggcggcc gacgcagtcc
     6721 gcgcgaccta cacgaaagaa aaacccaatg ttgaaacaca cctggaagcc ggcaatgatc
     6781 ctgacgtcgt cttcaccggc tatggaccag tgccacgact gcaaagtcag cgcggcgatc
     6841 cggacgccgc gtttgccagt gcgcccgtca agctcgacga gacttacagc acgccgcctg
     6901 aaacgcacaa tccactcgag ctgcacgcca ccaccgcgat gtgggacggc gacatcctga
     6961 cgctctacga ttcaacgcag ggtgtcgtta acatgcgcag cgttatggcg cagatgttcg
     7021 gtctcccgaa ggagaacgta cgcgtgatcg ccaagtttct cggttcggga ttcgggggca
     7081 agctctatcc gtggacgcac gtttcgctcg ccgccgcggc ggcacgtcag ttaggtaaac
     7141 cggtgaagct ggtcgtaagt cggaagatga tgttccagac cgtcgggcac cgcgcgcgta
     7201 cgcaacagcg catgcgcctg ggcgccaccc gcgagggcaa gctcctctca ttccagcacg
     7261 attacgtcta ccacctgtcg atgatcgacc attatcacga ggattgtggt gaggcttcgg
     7321 cgtttcatta cagtgttccg aacttgcgcg tgcactgggg gcgtgcccgg cgcaacatcg
     7381 gcgcgactgc tgacatgcgt ggcccaggcg ctgtgcccgg gctctatgcc accgaatcgg
     7441 caatgaacga aatggctttt cgactgaaga tcgatccggt gcagtttcgc cttctcaatg
     7501 agcccaagct cgatgagtca aacaacctgc aattttcatc acgacactac gtggagtgct
     7561 tgaatctcgg cgtggagaaa ttcggctggt ccaaacgcaa tccggccgtt ggctcgatga
     7621 agcgagacgg tttgacgttg ggctggggca tggccggcgc cgcgtggatc gcaggacgat
     7681 tcgcagccgc ggcgagcctg caactgcgcg acgacggaac cgtgcgcatc gcctgtggaa
     7741 cgcaggacat cggcacgggc acgtacacga ttctggcgca gctcgcgtca gagaagaccg
     7801 gagtgtcact cgataagatc gaagtggagc tgggtgacag tgcgcttcct gatggtccaa
     7861 tctcgggcgg ttcgctggct accagctcag tggtcccggc cgtctttcaa gctgccgacc
     7921 aggcgatcac atcgttgatg atcttggctg tcacgactcc gggctcaccg tttttaggac
     7981 gcaaacctga ggatctcgcg ctggaaggtg gtcgcatatt cgtcaaagcc gaaggagtcg
     8041 ggaagggagt tgcgtttggc gaggtgctgc ggaaagcgaa tgcacggctg gtcgcaggca
     8101 gcggcagtgc ggatcagacg gtttttgcgg cgaacgcgaa gttttctatg cactcgttcg
     8161 gatgccattt tgcggaggtc acgtggcagc cggagacggc acggttgcgc gtgagccggg
     8221 tggtgacggt gatcgatgcc ggaaggattt tgaatccact caccgggcgt aatcagatcc
     8281 agggcgcggt cgtgatgggt atcgggatgg cgttgtttga agagactgcc tacgatccgc
     8341 agaacggcgc gccgatcaat agcaacctgg ctgactac
//
LOCUS       DASSFQ010000134         8144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1542039, whole genome shotgun sequence.
ACCESSION   DASSFQ010000134 DASSFQ010000000
VERSION     DASSFQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542039"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1969)
                     /gene="asnB"
                     /locus_tag="VFH96_04625"
     CDS             complement(128..1969)
                     /gene="asnB"
                     /locus_tag="VFH96_04625"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6973283.1"
                     /translation="MCSISGILGRVSRDAVVRMNQAQRHRGPDDCGIASCGAVLLGNT
                     RLAIIDTSTAGHQPMNDPQTGNWITYNGETYNFKDLRHELDQEAWCSDTDTEVVLRAY
                     AKWGIDAFRKLCGMFALAIWDDQRQTLLLARDPLGIKPLYYYADDQQFIFASELRALL
                     ASGLVPRKLSTAGVDSYLANGSVAAPLTIIDGVKQLLPGHCLQVNGQIELRDIEFAIP
                     DSQSFDGNRDEAVARLRSELEESVRLHLLSDVPLGVFLSGGMDSSALVALITRISDQR
                     PKTFSVVFDEAAYTEAPFSRAVAERFKTDHSEIRLSEDRLLDILPTAINAIDQPTMDG
                     INTFVVSSAVKREGITVALSGLGGDELFAGYPSFRRALKFAAMSQGSKRFLRAASGVG
                     KFALNGSVQRQKFWQLLNSDGEPQDVYRISRQLFSTDAVAELTGREASNHSSNGHRHD
                     NDIVNTISRLELRGYMTNTLLRDTDAMSMAHSLEVRVPFLSVRLVDFVLSLPGEWKLR
                     GGDGPKPLLADVMSDLLPREFTNRPKMGFTLPFEKWMQGKMRAEIAAVLGDEQRLSLA
                     GLNSETVGKVWRRFLEKPKAVGWSRPWAIYVLVKWCETNGIRQFDVR"
     gene            complement(2041..3078)
                     /locus_tag="VFH96_04630"
     CDS             complement(2041..3078)
                     /locus_tag="VFH96_04630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6973284.1"
                     /translation="MRVFVLTDCPSPYQVELFNEIEAQGDCSLEVGYLRERDPDRRWS
                     SPEIRHASIDLKDARGADLVVFNYYRHANAERLIEERAASGGPWCFWGERPGFRQPAW
                     AGRLLRRWKLAKLHTSTVPIWGIGQFAVDGYREEFGAERAYFNLPYFSDLERFAVAAR
                     EEKRERTFLFSGSLIERKGVDLVAGAFVRLAQEVPNVRLKIVGEGELRESLVRTLRRV
                     SERVEFVGFKDWQELPAEYAAADVLCVPSRYDGWGLVVPEGLASGLPVIGTDRMGAAI
                     EFVETRWNGWLISAGDEEALLAAMRDAALMPADEIREMGCRARESVSAHTLRIGAMRF
                     VDYARQVVSQW"
     gene            complement(3075..4526)
                     /locus_tag="VFH96_04635"
     CDS             complement(3075..4526)
                     /locus_tag="VFH96_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HET6973285.1"
                     /translation="MQQGQLIHLPAFGMAATPSMTLRATLLALLSTTIATTMAVLLIP
                     DDPSTEGALFYPALVMSAGLATAPLFTALRYPKALLRGECLLSLAPIYWLLLDLLQGV
                     YAMEDVNADQVRQAFLAIGLFVVMVWLGALRRSWRIPKVLISSASQEFSVNTYFALAV
                     ACFVLGMLNFAIPCNFNVFEMVHYLGQERWAAPWGRGQLGGWDAFLDHLQYFGYLVPV
                     LTVVVGRRAGVRNTRTLICIVMSVIVALFLAQSGSRRVIGVVAGMALILWVLDQHRLR
                     VKHLVMTAVAIFALLLTLQVMLEYRNIGLSVLVGKGEVQSGWYEKRGILEEQHLRVDD
                     NFYRLCQIIQLIPDSYPFVYHKYLLYVIVRPVPRVFWPDKPVDPGFDLPTALGVQGLS
                     LSYSVIGELYMSLGFIGIALGGWFYGRVASTASGLLARCKTQGALVIYSIVVMALFSG
                     MRSILELILVSYVVLAWVGMSHLFMKLRHVRAR"
     gene            complement(4600..5751)
                     /locus_tag="VFH96_04640"
     CDS             complement(4600..5751)
                     /locus_tag="VFH96_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6973286.1"
                     /translation="MKWICCQLGAREHYAVPRALKLGGLLDELVTDLWMKRLIGRFHP
                     DLADARVVAPNIPALAFELKASFARENGWTLISRRNQWFQKQAVGQLAHNHNGNQHTV
                     FAYSYAAKEIFEFARARGWRTVLGQIDPGPAEERIVAGLQSPLAHTHWEPAPKVYWDN
                     WHDECSLADRIVVNSNWSRDALIAEGIAAEKIREIPVAYEAAEDTSSFQRQYPRSFSA
                     ERPLRVLFLGQVNLRKGVAQLLEAMKLLSGDHVEFWFVGPMQIRVPQDLKLHPQVRWL
                     GVAPRVSVESYYRDADVFILPTLSDGFGLTQLEAQAWKLPVIASRYCGEVVRDQVNGV
                     VLEEVSGEAIAEVIRGFLRSPERLSAMSVRSGVDERFSLTTLASSLRQL"
     gene            complement(5751..6620)
                     /locus_tag="VFH96_04645"
     CDS             complement(5751..6620)
                     /locus_tag="VFH96_04645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973287.1"
                     /translation="MKANISAIVTAYERVEQTLATLRVIQNCVPPPDEILVHVDANQV
                     ACENAIREVFPSVRVLRSEEQVGPGGGRNKLVEAAQCEFIASFDDDSYPIDSDYFARA
                     LQVFEKFPEASLICAALYHAGESIGLDEKSAHWTADFSGGACIYRRKAFLDAGGYVPL
                     PVAYGMEEVDLAIRLHSQGGKILTTRWLRVFHNTDLKRHGDPHVTAGSIANLALLAYL
                     RYPVSLWGIGIGQCANRLLWLLKHGRRRGIFKGVTMIPAYLWTNHRYRLPVSSKVVRS
                     YLALRRAPVEARL"
     gene            complement(6617..7483)
                     /locus_tag="VFH96_04650"
     CDS             complement(6617..7483)
                     /locus_tag="VFH96_04650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6973288.1"
                     /translation="MNCRVCNAETDPFMRISSDGLDEPFLDDTLRKQLPELKLRRCDR
                     CGCLWADDARQDEVTLVAAYERVSDSYFEPTENDPRFVGFYKDLDELLKEHVSGREIL
                     DVGCGDGTFLLTLSNDWSKHGIEPSVSGARLARERNLDVLCATLDTTPNAYEVDLISA
                     LDVIEHVVDPHNFIESLKRHLRKGGVVLLLTGDANAYPARIAGPQWSYLRWCGHISVF
                     SQAGLRRLLESHGFEVLTSRRCGHPSSPGAVAWWRVHLLEPARRILGRNKSWYPFWRD
                     HQTVIARLKDSY"
     gene            complement(7480..>8144)
                     /locus_tag="VFH96_04655"
     CDS             complement(7480..>8144)
                     /locus_tag="VFH96_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973289.1"
                     /translation="YAVSREMAAHYEQSFGKSWAVVHNGVRNDSLSETNGLSKPRQVL
                     LAGDVNVFRFDAVIAFAEAIERHNRRNGESIEFTVMGEVAEQHRSSLSGLVKMLDRRS
                     HAECLEAMKAADLLYLPLAFGEKSSRISRYSLPTRLPEYLATGKSVFFHAPQESAMFR
                     VAERYDLTPRLATIEPAALDAFVETWANGNDANCMDKAKRALREEFDIDTLAARFQAA
                     FT"
BASE COUNT         1952 a         2594 c         2010 g         1588 t
ORIGIN      
        1 gagccgaata ggatcgatca gaccacttag tgagctttga tcaatgatcg accaccttga
       61 agcagccatt tatcaacctt aatcagagtt tcctttagtt tgtcgcgaca ttccgacaag
      121 ctaaagctta tcggacatca aactgacgaa tgccattggt ctcacaccac ttcaccagca
      181 catagatcgc ccacggtctt gaccagccaa ctgcctttgg cttctccaga aatctgcgcc
      241 aaaccttccc aactgtttcc gaattcaatc ccgctagcga cagtctctgc tcatccccga
      301 gcaccgccgc aatctccgct cgcatcttgc cctgcatcca cttctcaaac ggcagcgtga
      361 atcccatctt cggccggttc gtgaactcac gcggcaacaa atcactcatc acgtcggcca
      421 gcaacggctt cggtccatct ccgccgcgca gcttccattc acccggaagc gacaacacaa
      481 aatcaaccag cctcaccgat agaaacggca cacgcacctc aagcgaatgg gccatgctca
      541 tcgcatcggt gtcgcgcaac aacgtgttcg tcatataacc gcgcaactcg agccgcgaga
      601 tcgtattgac gatatcgttg tcgtgccggt gaccgttgct cgagtgatta ctcgcctcac
      661 gcccagtcag ttcagcaacg gcatcggtgg aaaaaagttg tcgtgagatg cggtaaacgt
      721 cctgcggttc gccatcactg ttcagcaact gccagaactt ctgtcgctgc actgacccat
      781 tcaatgcaaa cttcccgaca cccgacgccg cgcgcaggaa gcgcttcgac ccttgcgaca
      841 tcgcagcgaa tttcagtgcc cgtcgaaagc tcggataacc cgcaaacagc tcatcgccac
      901 cgagcccgga gagcgccact gtgatccctt ctcgcttaac ggcactggaa acgacgaacg
      961 tgttgatgcc gtccattgta ggctggtcga tcgcgttaat ggctgtgggc aggatgtcga
     1021 gtaagcgatc ttcactgagc cgtatctcgg agtgatcggt cttgaagcgt tcggcaacgg
     1081 cacgcgaaaa cggcgcttcg gtgtacgcgg cttcgtcaaa cacgacgcta aacgtcttcg
     1141 gccgctgatc gctgatgcga gtgattaggg cgacgagcgc gctcgaatcc atcccgccgg
     1201 agagaaacac gccgagcggg acgtcgctca ggagatgaag tcgaacagat tcctcgagct
     1261 cggatcgtaa ccgggcgact gcttcgtcac gattgccgtc aaagctttga gagtctggaa
     1321 ttgcaaactc aatgtcgcgc aactcaatct gtccattcac ctgaaggcaa tggcccggca
     1381 agagctgctt gacgccgtcg atgatcgtga gtggcgctgc aactgagccg ttcgcgagat
     1441 acgaatcaac accggcagtg ctgagcttgc gcggcacaag accgctcgcc agcaacgcgc
     1501 gtaattcaga cgcgaagatg aactgctggt catcggcgta gtaataaagc ggcttgatgc
     1561 cgagcggatc gcgtgcgagt agcaacgtct gtctttgatc atcccagata gcaagtgcaa
     1621 acatgccgca cagtttgcga aaggcatcga taccccattt cgcataggcc cgcagaacaa
     1681 cttcggtatc tgtatccgaa caccacgctt cctggtccag ttcgtggcga agatctttga
     1741 agttgtacgt ttcgccgttg tacgtgatcc agttgccggt ttgtggatcg ttcattggct
     1801 gatgaccggc ggtcgacgta tcgataatcg caagcctcgt attgccgagg agaactgcgc
     1861 cgcagcttgc gatgccgcaa tcatcagggc cgcggtgtcg ctgcgcctga ttcatacgca
     1921 ccacagcatc acgcgacact cggcccaaaa tcccggaaat gctacacatc agttttacag
     1981 gattacagaa ttttcaggat gaacatgtaa atcttgttaa tcctgcaatc ctgtaaacaa
     2041 ctaccactga ctaacaacct gccgggcata atcaacaaac cgcatcgccc caattcgcaa
     2101 cgtatgtgca ctaacgctct cacgagccct gcaacccatc tcccgaatct cgtccgccgg
     2161 catcaacgcc gcgtcgcgca tcgcagcaag cagcgcctcc tcatcacccg cagaaatcaa
     2221 ccacccattc cacctagtct caacaaactc gatcgccgct cccatccgat cggtgccgat
     2281 caccggcaat cccgacgcta acccctccgg caccacgagc ccccaaccgt cataccgcga
     2341 cggcacacac aacacatccg ccgccgcata ctccgccggc aactcctgcc aatctttaaa
     2401 cccaacaaac tcaactcgct cactcaccct ccgcagcgtt cgcaccagcg actcgcgcaa
     2461 ctcgccttca ccaactatct tcaacctcac attcggaact tcctgcgcca gccgcacaaa
     2521 cgcacccgca accaaatcga cccccttgcg ctctatcaac gaacccgaaa acaaaaacgt
     2581 ccgctcgcgc ttctcttcac gcgccgccac agcgaaccgc tcgagatcgg agaaatacgg
     2641 caagttgaaa tacgcgcgct cagccccaaa ctcttcgcga tacccatcaa ccgcaaactg
     2701 cccgattccc cagatcggca ccgtcgacgt gtgcagcttc gcgagtttcc aacggcgcaa
     2761 caatcttccc gcccacgcag gctgtcgaaa ccccggccgc tcgccccaga aacaccatgg
     2821 cccaccagac gcagcccgct cttcaatcag tcgctccgca tttgcatgac ggtaatagtt
     2881 gaacacgacg agatcggccc ctcgcgcatc cttcaagtcg atcgacgcat gtcgaatctc
     2941 cggcgacgac caccgccgat ccggatcgcg ctcacgcaga taaccaacct cgagtgagca
     3001 atcgccctgc gcttcaatct cattgaacaa ctcgacctga tacggcgacg gacaatctgt
     3061 caaaacaaaa actctcaacg cgcccttacg tgtctcaact tcatgaacaa gtgcgacatg
     3121 cccacccatg caagcacgac gtagctcacc aggatcagct cgaggatcga tcgcattccc
     3181 gagaacagcg ccatcacgac gatgctgtag atcacgagcg cgccctgcgt cttgcagcgc
     3241 gcgagcagcc cactcgccgt cgacgccacg cgcccgtaaa accaaccgcc caaagcgatg
     3301 ccaatgaagc cgagcgacat gtaaagctcg ccgatcaccg agtaagaaag tgagaggccc
     3361 tggacgccga gcgccgtcgg caagtcaaat cccggatcga ccggcttgtc cggccagaac
     3421 acgcgcggca ccggtcgcac gatcacgtaa agcaaatact tgtgatagac aaacgggtac
     3481 gaatcaggaa tgagctgaat gatctggcag agtcgataga agttgtcatc gacgcgcagg
     3541 tgttgctctt cgagtatccc tcttttttcg taccagccgc tctgaacttc tcctttgcca
     3601 acaagcacgc tcaagccgat gtttctgtat tcaagcatca cctgaagcgt aagcagcagc
     3661 gcgaagatag cgacggcggt catcaccaga tgtttgacgc gcaaacggtg ctggtccaac
     3721 acccagagaa tcagcgccat gcccgccacg acaccgatca cccggcggct tccgctttgc
     3781 gcgaggaaga gtgccacgat taccgacatg acgatgcaga tgagcgtgcg tgtgtttcgc
     3841 acaccggcgc gccgcccgac gacgaccgtg agaaccggaa ccaggtagcc gaagtattgc
     3901 aggtgatcga gaaatgcgtc ccaaccgccg agctgaccgc gtccccacgg cgcggcccag
     3961 cgctcctggc ccaggtaatg gaccatctcg aagacgttga agttgcacgg gatcgcaaag
     4021 ttcaacatgc cgagcacgaa acaggcgacg gcgagcgcga agtaagtgtt gactgaaaac
     4081 tcctgcgacg cagagctaat caacactttc ggaatcctcc acgaccgacg cagcgcaccg
     4141 agccacacca tcacgacgaa tagaccgatt gcgagaaacg cctggcgcac ttgatcggca
     4201 ttgacgtctt ccatcgcgta aacgccttgc agcagatcga gcagcaacca gtagatcggc
     4261 gcgagtgata gtagacattc gccacgaagc aaagctttgg gataacgaag cgcagtgaag
     4321 agtggtgctg tagcaaggcc cgcggacatc acgagcgcgg ggtagaacaa tgcgccttcg
     4381 gtggatggat cgtcggggat cagaaggacg gccatcgtgg tcgcaatggt cgtgctgagc
     4441 agcgcgagca gcgtcgcgcg cagcgtcatg ctcggcgtcg cggccatgcc gaatgctggt
     4501 agatggatta gctgaccttg ctgcacgtga ttagtagaca ggatttacaa gatttttcag
     4561 aattaacatg taaatcctgt tcaatcgtgt gaatcctgtc taaagctgtc tcagcgatga
     4621 ggcgagcgtc gttaaactga agcgctcatc aacacccgat cgaaccgaca tcgcactcaa
     4681 tctctccggc gatctcaaaa accctcgtat cacctccgcg atcgcttcac cgcttacctc
     4741 ttcaagcacg accccgttaa cctgatcgcg caccacctca ccacagtagc gcgatgcaat
     4801 aaccggaagc ttccacgcct gcgcctcgag ctgcgtcaac ccaaacccat ccgacagagt
     4861 cggcagaata aacacgtctg cgtcgcggta ataactctcc actgacacac gcggcgcgac
     4921 gccgagccaa cgcacctgtg gatgcagttt cagatcctgc ggcacgcgaa tctgcattgg
     4981 cccgacaaac cagaattcga cgtggtcacc tgacaacaac ttcatcgcct cgagcaactg
     5041 cgccacacct tttcgcaggt tcacctgtcc gagaaacagg actcgcagcg gtcgctcagc
     5101 actgaacgaa cgcgggtact gacgttgaaa cgagctcgta tcttccgccg cttcatacgc
     5161 gaccgggatc tctcgtatct tctccgccgc gattccttca gcgatcagcg catccctgga
     5221 ccaattggag ttcacgacga tccgatccgc gagcgaacat tcgtcgtgcc agttgtccca
     5281 gtaaactttc ggggctggct cccagtgtgt atgtgctaac ggagactgca agccggcaac
     5341 aatgcgttct tccgcgggac cgggatcgat ctggccgagc accgtgcgcc agccacgcgc
     5401 gcgcgcaaac tcaaagatct ctttcgcggc gtagctgtag gcaaagactg tgtgctggtt
     5461 gccgttatgg ttatgtgcga gttggcccac cgcctgtttt tgaaaccatt ggttacgacg
     5521 actgatcagc gtccaaccgt tttcacgtgc gaatgatgct tttaactcga aagccagcgc
     5581 gggaatgttt ggagcgacga ctcgcgcgtc tgcgagatcc ggatgaaacc gcccgatcaa
     5641 acgcttcatc cagagatcgg taactaactc gtcgagcaat ccaccaagct ttagcgcccg
     5701 tggtacggcg tagtgctcac gcgcgccgag ttgacagcag atccacttca tcaaagacgt
     5761 gcctcgacgg gcgcccgtcg caatgccagg taagatctca cgactttgct gctaaccggc
     5821 aagcgatacc ggtgattggt ccacagatac gccgggatca tcgtgacgcc tttgaagatg
     5881 ccgcggcgcc ttccatgctt tagcaaccac aacaatcgat tcgcgcactg tccaataccg
     5941 atgccccaca gtgaaaccgg atatcgcagg tacgccagca gcgcgaggtt cgcgatgctt
     6001 ccggcagtca catgcggatc gccgtgtcgc ttcaagtcag tgttatggaa tacgcgcagc
     6061 catcgtgtag ttaagatctt tccgccctgc gagtgcagcc ggatcgcgag atccacttct
     6121 tccatcccat acgccacggg cagcggcacg tatcctcctg catcgagaaa tgcttttcgg
     6181 cggtagatac acgcgccgcc ggaaaaatcc gcggtccagt gggcgctttt ctcgtccagc
     6241 ccgatcgact caccggcgtg ataaagagcc gcacaaatca acgaagcctc gggaaacttc
     6301 tcgaagactt gcaaagcgcg ggcgaagtag tctgagtcga tcggataaga gtcgtcatcg
     6361 aagctcgcga taaactcaca ctgcgccgcc tcaaccagct tgtttcgtcc cccacccgga
     6421 ccaacctgct cctcgctgcg aaggactcga acactcggaa acacctcacg aatcgcgttc
     6481 tcacacgcga cttgattggc gtcaacatgg accagtattt catccggcgg tggaacacag
     6541 ttctgtatca cgcgcagcgt cgcaagcgtc tgctcgacgc gctcataagc tgtgacgatt
     6601 gcagaaatat tggccttcaa tacgaatcct tcaggcgcgc aattacggtt tgatgatctc
     6661 gccagaacgg ataccacgac ttgtttcgtc cgaggatgcg acgagcaggt tccaacaaat
     6721 ggacgcgcca ccacgcgact gctccaggac tcgacggatg cccgcaacgt cgcgatgtaa
     6781 gcacttcgaa tccgtgtgac tccagcagcc ttcttaaacc tgcttgcgaa aacaccgaga
     6841 tgtgaccgca ccatctcaga tacgaccact gtggaccagc aattcgcgcc ggataagcat
     6901 tagcgtcgcc ggtcaaaagc aacacgaccc cgccttttcg caggtgtcgc ttgagagact
     6961 ctatgaagtt gtgtggatca accacgtgct cgatcacgtc cagcgccgag atcagatcaa
     7021 cctcgtacgc attcggagtt gtatcgagcg tagcgcacaa gacatcaaga ttccgctctc
     7081 gcgccaaccg cgcccccgac acgctcggct caataccgtg cttggaccaa tcgttcgaga
     7141 gcgtcaataa aaacgtgcca tccccacaac cgacatcgag gatctccctg ccggagacgt
     7201 gttccttcaa tagctcatcg aggtccttat agaacccgac gaatcgagga tcattctccg
     7261 tcggctcgaa gtaagagtcg cttacccgct catacgcagc gaccagtgtt acttcatcct
     7321 gtcgcgcgtc atccgcccac aaacatccac atcgatcaca gcgtcgcagt ttcaattcgg
     7381 gaagttgttt gcgtaacgtg tcgtcaagaa acggttcgtc gagaccatcg ctggagattc
     7441 gcatgaacgg atcagtctcg gcattgcaaa cccggcaatt catgtaaacg cagcctgaaa
     7501 acgcgccgcc agcgtgtcga tgtcaaactc ttctcgcaac gctctcttcg ctttgtccat
     7561 gcaattggcg tcattcccgt tcgcccacgt ttccacaaac gcatcgagcg cggcgggctc
     7621 gatcgtcgcc aaccgcggcg tcaagtcgta acgctccgcg acccgaaaca tggcagactc
     7681 ctgcggcgca tgaaagaaca cgcttttgcc cgtcgctaaa tattcgggaa gcctcgtcgg
     7741 caacgaatag cgcgaaattc gtgaagactt ttcgccaaac gcgaggggca gatagagcag
     7801 gtccgcagct ttcatcgcct cgagacactc tgcatgcgaa cgccggtcca acatcttcac
     7861 taaacctgac aatgaggacc ggtgttgctc ggccacttca cccatcacgg taaactcgat
     7921 cgattcgccg ttacgccgat tgtgacgctc gatcgcttcg gcgaaagcaa tcacagcgtc
     7981 gaagcgaaag acattcacgt caccggcaag cagcacttgt cgtggcttcg ataaaccatt
     8041 cgtctcactc agcgaatcgt tgcgcacacc gttgtgcacc acagcccagc tcttcccaaa
     8101 actctgctcg taatgcgccg ccatctcacg agaaaccgcg taac
//
LOCUS       DASSFQ010000135        13373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_647605, whole genome shotgun sequence.
ACCESSION   DASSFQ010000135 DASSFQ010000000
VERSION     DASSFQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13373
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647605"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2311)
                     /locus_tag="VFH96_04660"
     CDS             complement(<1..2311)
                     /locus_tag="VFH96_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973290.1"
                     /translation="MLRSGLLLMLALSYVDPSFFNGLRYRLVGPARGGRVTTVTGVPS
                     QPKTFYMGVASGGLFRTTDSGATWTPITDGKVPLGSTGSVAVADSDPNVIYLGTGSDG
                     VRSNVSTGRGMYKSTDGGQTWKFIGLYNAGQIGAVRIHPTNPDIVWVAVNGDIFKPTD
                     ERGVFKTTDGGQTWRKVLFVSNGIGAMDIELQPNNPNVVYAWMSRLERKPWTIISGAR
                     EGGFYKSTDGGEHFTKISNGLPNELIGKANLAVTNANPNRIYALVEAKPGGGFYRSED
                     AGQTWALINSSGALIQRPFYYTTLGADPSNADVVYAGAEGFFKSTDAGKTFTPFRTPH
                     GDNHDIWINPKDSQIMIQSNDGGANVSTDGGRTWSSQMSQPTAEIYGIWVDNQFPYKL
                     YGAQQDNTTLIISSQAEPFSTNDWRPGPGCETGPIMPHPANPDIVYGSCKGQYGVMNL
                     KTGQEKSYWIGGQSLYGNPASDLIYRMQRVSPMATSPHDPDVLYYGSQFVHRTRDKGV
                     TWERISPDLTAHPACCQGVSGEPITRDVTGEEFYSTLYAITESSLEKGVIWTGSNDGP
                     FYVTRDNGKRWTNVTPKDLPPGGRVQYIEASPHRKGSAYFAVYRWLLGDFEPYIYLTN
                     DYGNTWTRLTNGKNGILADWPTRVVREDPDREGLLYAGTEFGMFISFDNGAHWQPFQL
                     NLPNVPVSDIKVHHKDLIVSTQGRAIWILDNVTSLHQLQSPMKTSEAKLFKPRDAYRT
                     RVSPAVLGPVVEYYLPAAPSDAVLIEILDA"
     gene            2385..3242
                     /locus_tag="VFH96_04665"
     CDS             2385..3242
                     /locus_tag="VFH96_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6973291.1"
                     /translation="MEHIKTAPQLPSERDKNYLLTDDSGQQLVLKVANIAESRDFLEA
                     QNAVLDYLAERVSFCQRVIGEIAEFEGHLVRVVTYLPGVPLAEIKPHTPGLLHDLGQK
                     IGQLTRALADFDHPAVHRDFHWDLANGNRVVNEYAPLIEDAKLRELVLKCRFEPATEL
                     RRSVIHGDANDYNVLVDPESMTVSGLIDFGDMVYSYTIGDLAIAVAYVVLGEGDPRDV
                     IAGYRSEFGLLDEEREALWPLVRLRLAMSVCIAAHQIRQQPENEYLRISQKAIEKTLP
                     RIFTDEYTD"
     gene            3226..4431
                     /locus_tag="VFH96_04670"
     CDS             3226..4431
                     /locus_tag="VFH96_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6973292.1"
                     /translation="MNTRIDGLIDRRRILLGRNLSVAYERPVHIVRGEMQYLYDAEGR
                     QYLDAYNNVAHVGHCHPQVVAAGQRQMEVLNTNTRYLSELILEYAEKLTATLPESLSV
                     CFFVNSGSEANELAIRLARAHTKARSLIVLEHAYHGNTTTCIDISPYKHDAAGGEGAP
                     PWVHTARLADAEHAIEIVQKLEAPLCGFIAESMPSVAGQIIFPDGYLNQVYEAVRRAS
                     GVCIADEVQTGFGRTGTHFYAFERYGVVPDIVVLGKPIGNGHPIGAVITTRAIADSFD
                     NGMEFFSTFGGNNVSCAIGLKVLEVVHEENLKAHALHVGNHLLAGLHELNPRHEVIRE
                     IRGSGFFLGVELVEAAHSVVNQIRERGILMGTEGPLHNVIKIRPPMPFSMEDADRLIE
                     TLDEVLTRG"
     gene            complement(4418..4768)
                     /locus_tag="VFH96_04675"
     CDS             complement(4418..4768)
                     /locus_tag="VFH96_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973293.1"
                     /translation="MPQYEPEAWRDGHEPNGDPSKREQCKLLDLLGRINYRILSGNRV
                     YVEVGGVVYKTVYGGYWRSVFTGIEHYLLHCEQVSSAVWKDENTTAVDLPIAYSNGGW
                     TTRGHSLSFILNRE"
     gene            4945..5550
                     /locus_tag="VFH96_04680"
     CDS             4945..5550
                     /locus_tag="VFH96_04680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6973294.1"
                     /translation="MIAGPIIYVALGDSTGYGVGARDGGYVKRLFDRINERRPTSTLL
                     NLCVSGATTDDLVRGQLDRAVELNPDLVTVGIGINDIGHGLTLEQFSKNYETILSTLK
                     EKTHAQIVVTNLPDVSSAPRIPDSMRSTYQRQIDQFSQRLMEIAERYGVTIFDIYTIT
                     KDELPSHPEYFSNDGFHPSDEGYELWATQMWPTVAKVIGEP"
     gene            complement(5606..6595)
                     /locus_tag="VFH96_04685"
     CDS             complement(5606..6595)
                     /locus_tag="VFH96_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973295.1"
                     /translation="MGIEFRNVTKTFGGACVVDDLSLEVRDGEFIVLLGPSGCGKTTT
                     LRMLAGLETATSGDILINGERVNDVPTQHRDLAMVFQSYALYPHMTIAENIGYPLRVR
                     KLDKVDRSARVQRVAEMLEIGPLLERKPRQLSGGERQRVALARAIVREPRAYLMDEPL
                     SNLDARLRVQMRGELKRLQHQLGTTTIYVTHDQAEAMTLASRVAVMKKGRLQQLDTPL
                     NIYNHPANRFVAEFVGSPSMNFIDGERGTMGIRPEHIQVLTAPEDGAMEARVYVTELM
                     GNETFVFVSVGDNRLIARAPADFRADVDTKVWLKLATDKIHYFDPKSGQSCKS"
     gene            complement(6596..7420)
                     /locus_tag="VFH96_04690"
     CDS             complement(6596..7420)
                     /locus_tag="VFH96_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6973296.1"
                     /translation="MIKRVSIYLALVGALLFALAPVYWMLTISFKTEGEQFASPPPWF
                     VFTPTLEHYYDAFVTRSFGQYLLTSAIVAVTSTACALVIGTLAAYALARFELPLKLNR
                     RLSLWILSTRMFPAIVTAVPLFLMMRDVQLLNTRAALIIVYTTFNLPFVVWMMRGFFA
                     DLPRDLEEAALVDGDSRLGALVRVVLPLVAPGLAATAVFCLIVSWNEFLFALVLTQTD
                     AAMTLPVGIAGRVTQYEIKWGVMSAAASVAIVPILVFALALQKYLVRGLSMGAVKG"
     gene            complement(7491..8384)
                     /locus_tag="VFH96_04695"
     CDS             complement(7491..8384)
                     /locus_tag="VFH96_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6973297.1"
                     /translation="MPRSRRFFNAQNKRELLLPALFLAPALAVLLALSIYPLIYSVTI
                     SLQQETAAGITWTLGNFTRLFSDNFFWTAMGHTFIYAAVALTCEFLLGLSLALLLNNQ
                     IRGRGFFRASLLVPMMLPTVVVGVVWRLMLNPNFGAVNGTLKQFGIDTESLTWTASPK
                     LAMLSVIAVDVWQWTPFVFLVLLAGLQAIPQEPYEAALIDGSSRWQTFWHVTLPLLKP
                     SILIVLLLRTMDLLRVFDQIFILTEGGPGFATETISLYIYRAAFRFFDFGYAAAMSFV
                     LLALTNVISAAYIRLLQAKET"
     gene            complement(8347..9612)
                     /locus_tag="VFH96_04700"
     CDS             complement(8347..9612)
                     /locus_tag="VFH96_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6973298.1"
                     /translation="MRILLLTLLLLLAISCTSQPSQKQLIVAVISGAEGEALKQAARD
                     YEAKTGTHIEIAQFPYANLFEKELIDLKSHTGAYDLIMLDDPWFPRFAGKRFLTDLTP
                     LLQKRGQSGPDSDFVEASLNVCRHPFQTGALFALPYVGNSQLFFYRKDLFEKYSLKAP
                     ATWDDVLAAAKTIHEKETAGANGSKVYGYVMRAAQGNAAVADFMPIFWAFGGEMFDAN
                     GKPAVNSPEGIAALEFMIELGKYSPPGYASFNADEVAAHLLQGTAAMSINWPVWISPF
                     SDPSRSKVIGRMEFTTLPGAKKPGQAEIGNWLIAIPRYSKNAEEAMDFLLWATAPEQM
                     KVSTRRGNPPTRRSLFNDAELVAKNPAYPAQLRSLESSRPRPRTPYWNEIENAFGIFL
                     SKANSGELPAAEAMNQANAEIEKILQRTK"
     gene            9669..10685
                     /locus_tag="VFH96_04705"
     CDS             9669..10685
                     /locus_tag="VFH96_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HET6973299.1"
                     /translation="MRQIVTTANGDIDVLKVQEKPDPTPGDDEVVIRVRAAGLNFADI
                     LARQGLYPDGPPKPCVLGYEVSGVVDSVGKNVNRSFVGKSVVALTRFGGQSEMIAVKA
                     TQMFEKPEQLTFEQAAAIPVNYLTAYALLVVMGSLHENESVLIHNAGGGVGLAALDIA
                     KKIGAVTYGTASQGKHKFLTDRGLDHPIDYRNQDWQPQLMQLTNGHGVDLIIDPIGGV
                     HWKKSYNALRHTGRLGMFGVSAASANGISGKLKMLRAVVQMPRFHPLGLLNKNRGVFG
                     LNLGHMWHEPEKVALWMRDILRGVEEEWINPHVDQAFLFDEVGKAHRRLEERKNIGKV
                     VLIP"
     gene            complement(10748..11200)
                     /locus_tag="VFH96_04710"
     CDS             complement(10748..11200)
                     /locus_tag="VFH96_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973300.1"
                     /translation="MKIPLKYGLLITLGVMAWVLITHSLIPNPQSLVHQLGGPIFFNV
                     LQFAMIYLGLKALEHAKGDRPYFKEGLKTGVAISFVYALSASIFFVVVLATVGTKWLA
                     SEPGATRAPISRIALSAFIGLFLLAMLFGLVYSTVLSFFLAKRRSEES"
     gene            complement(11238..>13373)
                     /locus_tag="VFH96_04715"
     CDS             complement(11238..>13373)
                     /locus_tag="VFH96_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973301.1"
                     /translation="FPNLAPEDFAPWVEEGVDAAQQAQLWKEGLAKWGEDGERIKRLR
                     DAADFRIYTPGSNAGLPVSILKSFVAPPETIRQDNEALAERINTTVTSLLALIGINAD
                     PVRSREHILLSNILNHEWSAGKDLDIASLIQNVQTPPITKVGVMELDSYFPADARFEL
                     AMSLNHLLASPSFASWMEGESLDVAKFLHTESGKPRHSIFSIAHLSDAERMFFVSLLL
                     NQVLGWMRTQSGTTSLRAILYMDEIFGYFPPVANPPSKLPLLTLLKQGRAFGLGVVLA
                     TQNPVDLDYKGLANTGTWFIGRLQTERDKARVLEGLEGIAAGTGQKFDKQAMEQLLAG
                     LDKRIFLLNNVHDDVPEVFETRWALSYLRGPLTRAQIKLLMDPLKATAAPAAPVSAPA
                     PVASAPPKSERAILPPDVSQYYIPVRSSGAAGETLTYHPMLLGTAEVRYSNSKTVEMT
                     QQVNLLAAFADGPAGLDWGKATASDLPVEDLESEPEAGAQFGEVPSEATKAKSYASWR
                     KDLASWVYRNQRLELLESPTLEIVSNPGENERDFRVRLQQLAREQRDEAVEKLRRKYA
                     PKFEALEDRKRRAEQAVERESEQAKGQKMQTAISFGATLLSSFLGRKRTSMSTLGRAT
                     TAARGVGRSMKESQDVERAQDNVEAISQTLSNLEADFQAATKALERSLDPQTEQLEKV
                     SLKPTKANIAVKVLTLAWAPYWDDKPAWE"
BASE COUNT         2961 a         3634 c         3866 g         2912 t
ORIGIN      
        1 tcgcgtcgag aatctcgatt agaacagcgt cactcggcgc cgccggcaaa taatactcaa
       61 ctactggtcc aagcactgcc ggactgactc gcgtgcgata cgcatcacgt ggcttgaaca
      121 gcttggcttc tgaagttttc attggcgact ggagttggtg cagcgaggtg acattgtcga
      181 gaatccagat cgctcgaccc tgcgtcgaga caatcagatc cttgtgatgc accttgatgt
      241 cgctcaccgg cacgttcggt aagttcaact gaaacggctg ccaatgcgcg ccgttgtcaa
      301 acgaaatgaa catgccaaac tctgtcccgg cgtaaagcaa gccttcgcga tccggatcct
      361 cacgcacgac ccgtgtgggc cagtctgcca gaatgccgtt cttaccgttc gtgagcctgg
      421 tccaggtgtt gccgtaatcg ttggtcagat agatgtaagg ctcgaagtca ccgagcagcc
      481 agcgatacac ggcgaaatac gccgatcctt tgcgatgcgg tgacgcttcg atgtactgca
      541 cgcgcccacc gggtggcaga tccttcggcg tgacgttggt ccaccgcttg ccgttgtcgc
      601 gcgtaacgta aaacggacca tcattcgatc cggtccagat cacgcccttc tcgagcgacg
      661 actcagtgat cgcatacagc gtgctgtaaa actcttcacc ggttacgtcg cgtgtgatcg
      721 gctcaccgct cacgccctgg caacacgccg gatgcgcagt cagatcgggc gagatgcgtt
      781 cccacgtcac gcctttatcg cgcgtgcgat gtacaaactg ggatccgtag tagagcacat
      841 ccggatcgtg cggcgacgtc gccatcggcg acacgcgctg catgcgatag atcagatcgc
      901 tcgcaggatt gccatacaac gactgcccgc cgatccagta gctcttttcc tgaccggtct
      961 tgagattcat cacgccgtac tgtcccttac acgaaccgta aacaatgtcg gggttcgccg
     1021 ggtgcggcat gattggacca gtttcacaac caggacccgg gcgccaatcg ttggttgaaa
     1081 aaggctcggc ctggctcgag atgatcagcg tcgtgttgtc ctgctgcgcg ccgtagagct
     1141 tgtaaggaaa ctgattatcg acccagatgc cgtagatctc cgcggtcggt tggctcattt
     1201 gcgacgacca cgtgcgaccg ccgtcagtcg agacattcgc gccgccgtcg tttgactgga
     1261 tcatgatctg gctgtctttc ggattgatcc agatgtcgtg attgtcgccg tgcggcgtgc
     1321 gaaacggggt gaacgttttg cccgcgtcgg ttgatttaaa gaagccttca gcgccggcgt
     1381 agacgacgtc tgcgttcgaa ggatcggcac cgagcgtcgt gtagtaaaac ggacgttgga
     1441 tcaatgcgcc agacgaattg atcagcgccc acgtttggcc tgcgtcttcc gaacgataga
     1501 aaccgccgcc gggtttggct tcgacgagtg catagatccg attcggatta gcattcgtga
     1561 cagcgagatt tgctttgccg atcaactcat tcggcaaacc gttcgaaatc ttcgtgaaat
     1621 gctcgccgcc gtcagtgctt ttgtagaaac caccttcacg cgcgccgctg atgatcgtcc
     1681 agggcttgcg ctcgagccgc gacatccacg cgtaaacaac attcggattg ttcggctgca
     1741 actcaatatc catcgcgccg atgccgttag agacaaacaa cactttgcgc cacgtttgcc
     1801 cgccgtcagt tgttttgaac acgccacgct cgtcggttgg tttaaagatg tcaccgttta
     1861 ccgcgaccca gacgatgtcc ggattcgtcg gatgaatgcg aaccgcgccg atctgcccgg
     1921 cgttgtagag accgatgaat ttccaagtct gtccgccgtc ggtagatttg tacatgccgc
     1981 ggccggtgga gacgttgctg cgcacgccgt cggagccggt gccgagataa atcacattcg
     2041 gatcggaatc agcaacggcg acagatccgg ttgacccgag tggaacttta ccgtcggtga
     2101 tcggcgtcca ggttgcgccg ctgtcagtcg tcctgaataa gccgccgctc gcgacgccca
     2161 tgtagaacgt ttttggttgt gaaggaaccc cggtcacggt agtcacgcgc ccgccgcgcg
     2221 ctggaccaac gaggcgatag cgaagtccgt tgaagaatga tggatcgacg tacgagagcg
     2281 ccaacatcaa gagcaagcca gagcgaagca tcagttttct cctatccgca gattacgcag
     2341 attacactga tttggtctaa tctgtgtaat ctgtgaataa gcttttggaa cacatcaaaa
     2401 cggcgcccca gttgccgagc gagcgagaca agaactatct gttgactgat gattcaggtc
     2461 agcagcttgt acttaaagta gcgaacatcg cggagtcgcg cgatttcctc gaggcgcaga
     2521 atgcggtgct ggattacctc gcagagcgtg tttcgttttg ccagcgcgtg atcggcgaga
     2581 ttgctgaatt cgaaggccat ctggtgcgcg tagtaacgta tcttccgggt gtgccactcg
     2641 cggagatcaa gccgcacacg cccggactgt tgcacgatct gggtcagaag atcggacaac
     2701 tcacgcgcgc actggctgac ttcgatcacc cggccgtgca ccgcgatttc cactgggacc
     2761 tcgcgaacgg aaatcgcgtc gtgaatgagt atgcgccgct gatcgaagac gctaagctgc
     2821 gtgagctcgt tttgaagtgc aggtttgagc cggcgactga gctgcgacga agcgtgattc
     2881 atggtgatgc gaacgactac aacgtgctcg tcgatccgga aagtatgaca gtatccgggc
     2941 tgatcgattt cggagacatg gtttatagct acacgattgg tgatcttgcg atcgcggtag
     3001 cgtatgtggt gttgggtgaa ggtgatccgc gtgacgtgat cgcggggtac cggagcgagt
     3061 ttgggttgct tgatgaggaa agggaagcgc tgtggccgct cgtgcggttg cgattagcga
     3121 tgagtgtttg tattgcagcg catcagatcc ggcaacagcc cgagaatgag tatctgagga
     3181 tcagtcagaa ggcgatcgaa aaaactttgc cacggatttt cacggatgaa tacacggatt
     3241 gatggcttga tcgacaggcg cagaattcta ctcgggcgca atttgagtgt tgcgtatgag
     3301 aggccggtgc acattgtgcg tggtgagatg cagtatctgt atgacgccga ggggcggcag
     3361 tatctcgatg catataacaa cgtcgcgcat gttgggcact gtcatccgca ggttgttgcg
     3421 gccgggcagc ggcagatgga agtgttgaat acgaatacgc gctacctgag tgagctgata
     3481 cttgagtatg cagagaaatt gacggcgacg ttgcctgagt cgttgagtgt ttgtttcttc
     3541 gtcaactccg gtagtgaagc gaatgagctc gccattcgcc ttgcacgtgc gcacacaaaa
     3601 gcacgcagtt tgatcgtgct ggagcatgcg tatcacggaa atacgacgac ctgcattgat
     3661 atcagtcctt ataaacatga tgccgctggt ggtgagggcg cgcctccgtg ggtgcataca
     3721 gcgcggcttg cagacgcaga gcacgcgatc gagatcgtgc agaaattgga agcgccgttg
     3781 tgtggattca tcgcggaatc aatgccgagt gttgccggac agatcatatt cccggatgga
     3841 tatctcaatc aggtttacga agcggtgcgc cgagccagcg gtgtttgcat tgcggatgaa
     3901 gtgcaaacag gatttggacg caccggcaca catttttatg catttgaaag gtacggcgtc
     3961 gtgccggata tcgtcgtgct cggaaaaccg atcggcaatg gacatccgat cggcgcagtg
     4021 atcacgaccc gcgccatcgc cgactcgttt gataacggaa tggaattctt cagcacgttc
     4081 ggcggaaaca acgtgtcatg cgcgatcgga ttgaaagtcc tcgaagtcgt ccatgaagag
     4141 aacctcaagg ctcacgccct ccacgtcgga aaccatctgc tcgccggcct acacgaacta
     4201 aatccgcgtc acgaagtgat ccgcgaaatc cgcggctccg gtttttttct tggagtggaa
     4261 ctcgtcgagg ctgcacacag tgtcgtaaat caaatacgtg agcgcggcat actgatggga
     4321 accgaaggcc cgcttcacaa cgtgatcaag atcagaccac ccatgccatt ctctatggaa
     4381 gacgcggatc gtctcatcga gactcttgat gaagtactta ctcgcggttg aggataaagg
     4441 aaagtgagtg tccgcgcgtg gtccaaccac catttgagta agcgatcgga agatcgacgg
     4501 cagtcgtatt ctcatctttc cacaccgcgc tcgacacctg ttcgcagtgc aaaaggtaat
     4561 gctcgatgcc ggtgaagacg ctgcgccagt agccgccata aaccgtctta tagaccacgc
     4621 caccaacctc aacataaacc ctgttgccac tcagaatccg gtagttgatg cgcccgagca
     4681 gatcgagaag cttgcactgt tcgcgcttcg acgggtcgcc gttaggctcg tgaccgtctc
     4741 gccatgcttc gggctcgtat tgcggcatta tgcgccgcac attattctcc tgttgggata
     4801 aagtcaagaa aaatggcagc ttcatcaatc aaggcggaca aaaagcttct acattttgca
     4861 tttgctctca catttcttgg aggaagtgtg cttgcactgc ttttcacggg gtgtaatcga
     4921 caagggggag cggaaagaag accaatgatt gcagggccta ttatatatgt cgcgctcggg
     4981 gattcgacgg gttacggtgt gggggcgagg gacggcgggt atgtgaaacg gctcttcgat
     5041 cgtatcaacg agcggcgacc aacttcgacg ttgctgaacc tctgcgtgag tggtgcgacg
     5101 actgatgacc tggtacgagg tcagttagat cgcgccgtgg aactcaatcc ggatctcgtg
     5161 acggttggaa ttggtatcaa cgacatcgga cacggattga cgctcgaaca gttttcaaaa
     5221 aattacgaaa cgatactcag cacgttgaaa gaaaaaacgc acgcgcagat cgtggtgacg
     5281 aatcttccgg atgtctcgag tgcgccgcgc attccggatt cgatgcggag cacgtaccag
     5341 cggcagatcg atcagttttc tcagcgactc atggagattg cggagcgata tggggtgacg
     5401 atcttcgata tttacacgat cacgaaggat gagctgccgt cgcatccgga gtatttttcg
     5461 aacgatgggt ttcatccgtc agatgagggg tatgagttgt gggcgacgca gatgtggccc
     5521 acggtggcga aggtgattgg tgaaccatag tagggtcaaa atcctgttga ttctgtaaat
     5581 cttgaaaatc atgtctaaca ggatgtcagg atttacagga ttgaccagat tttggatcaa
     5641 agtaatggat cttgtcggtt gctagtttca accacacctt cgtgtcgaca tccgcgcgga
     5701 agtcggcagg tgcgcgcgcg atcagccggt tatctccaac tgaaacaaac acgaaagtct
     5761 cgtttcccat caactcagtg acgtaaacac gcgcctccat ggcgccgtcc tcgggtgcgg
     5821 tcaacacctg aatgtgctca ggtcgaattc ccatcgttcc gcgctcacca tcaatgaagt
     5881 tcatactcgg actgccgaca aactctgcca cgaatcgatt cgccggatga ttgtaaatgt
     5941 tgagcggcgt gtcgagctgt tgcagccgtc ctttcttcat caccgcgacg cgtgacgcga
     6001 gtgtcatggc ttccgcctga tcgtgtgtga cgtagatcgt ggtcgttccg agctgatgtt
     6061 gcagacgttt caattcgcca cgcatctgca cgcgcaaccg cgcgtcgaga ttcgagagtg
     6121 gctcgtccat caagtaagca cgcggctcac gcacgatcgc acgcgcgagc gccacgcgtt
     6181 gtcgctcgcc gccggatagc tgccgcggtt ttcgttcgag tagcggtcct atctctaaca
     6241 tctctgcgac tcgctgtaca cgtgcagaac gatccacttt atcgagtttt ctgacgcgca
     6301 acggatagcc gatgttctca gcgatcgtca tgtgcgggta aagcgcgtag ctctgaaaca
     6361 ccatcgccag atcgcgatgc tgtgtcggca catcgttgac acgttcaccg ttaatcagga
     6421 tgtcgccgga cgtggcggtt tcgagaccgg cgagcatacg caaagtcgtc gtcttgccac
     6481 agcccgatgg cccaagcagc acgatgaact caccgtcgcg cacctcgagc gagagatcgt
     6541 cgaccacgca tgcgccgccg aacgttttgg tcacgttgcg aaattcgatt cccatctaac
     6601 ctttcactgc tcccatcgac agtccgcgca cgagatattt ctgcaacgcg agcgcgaaca
     6661 ccagaatcgg aacgatcgcc accgacgctg ccgcgctcat cacgccccac ttgatctcgt
     6721 actgcgtcac gcgtcccgcg attccgacgg gcaatgtcat cgccgcgtca gtctgcgtca
     6781 acacgagcgc aaacagaaat tcattccacg aaacgatcaa acaaaagacg gccgtcgcag
     6841 cgaggcccgg agcgacgagc ggcaagacga cgcgcacgag cgcgccgaga cgcgagtcgc
     6901 catcgacgag cgcagcctct tcgagatcgc gcggcaggtc tgcaaaaaaa ccgcgcatca
     6961 tccagaccac aaaaggaaga ttgaaggtcg tgtagacgat gatcagcgcg gcgcgcgtgt
     7021 tgagcaattg cacgtcgcgc atcatcagga agagcgggac ggccgtgacg atcgcgggaa
     7081 acattcgcgt cgaaaggatc cacaaagaga gtcgccggtt gagtttgagc ggcagctcga
     7141 accgcgcgag cgcataggcc gcaagcgtgc cgatgacgag tgcgcaggca gtcgaagtga
     7201 cggccacgat cgcgctcgtc agtaaatatt gtccaaagga acgcgtgacg aatgcgtcgt
     7261 agtagtgttc gagcgtgggc gtgaagacga accacggcgg cgggctggca aactgctcgc
     7321 cttccgtttt gaacgagatc gtgagcatcc agtaaaccgg cgcgagcgca aacagcaatg
     7381 caccgaccag ggccaaataa atcgagacgc gcttaatcat cagctatttt cgccacaaga
     7441 agcacaaaag gcacaaagta ttcttttgtg ctttttgtgc cttttgtgta ctaagtttcc
     7501 tttgcttgca ggaggcgaat gtatgcggca ctgatcacgt tcgtcagcgc gagcagcacg
     7561 aacgacatcg ccgctgcgta tccaaaatca aaaaaccgaa acgccgcgcg atagatgtat
     7621 agactgatcg tttcagttgc gaaacctggc ccaccttcag tcagaatgaa aatctgatcg
     7681 aacacacgca gcagatccat cgtgcgcagc aacaacacga tcaagatcga cggtttcagt
     7741 agcggcagcg tcacgtgcca gaacgtttgc caccggctcg acccgtcaat cagcgccgcc
     7801 tcgtatggct cctgcggaat cgcctgtaaa cccgccagca acaccagaaa cacaaacggc
     7861 gtccattgcc acacatcgac tgcgatcacc gacaacatcg cgagcttcgg cgacgcagtc
     7921 caggtcaaac tctccgtgtc gatcccaaac tgtttgagcg tcccattcac agcgccgaaa
     7981 tttggattga gcatcagtcg ccaaacaacg cccacaacca ccgtcggcag catcatcggc
     8041 acgagcaaac tcgcgcgaaa gaaaccgcgc ccgcgaatct ggttattgag caggagggcg
     8101 agactgagcc cgagcagaaa ttcacacgtc aatgccactg ccgcgtagat aaacgtgtga
     8161 cccatcgctg tccagaagaa attgtctgag aagaggcgcg tgaagtttcc gagcgtccac
     8221 gtgatcccgg cggcggtttc ctgttgcaga ctgatcgtga cggaatagat gagcgggtag
     8281 attgaaagcg cgagaagcac cgcgagcgcc ggcgcgagaa agagtgcggg cagcagcagc
     8341 tcgcgcttat tttgtgcgtt gaagaatctt ctcgatctcg gcattcgcct gattcatcgc
     8401 ttcggcagcc ggcagctcgc cgctatttgc tttcgaaaga aagatgccga aggcgttctc
     8461 aatttcattc cagtaaggcg tacgcgggcg tggtctcgag ctttcgagcg atcgcagctg
     8521 tgccggataa gcgggattct ttgcgaccag ctcagcatcg ttgaaaagcg agcgccgcgt
     8581 cggtggattg cctcgccggg ttgacacttt catctgctcg ggagcggtgg cccaaagtaa
     8641 aaagtccatc gcctcttcgg cattttttga gtagcgcggt atcgcgatca gccagttacc
     8701 aatttcagcc tggccgggtt tcttcgcacc cggcagcgtc gtgaattcca tcctcccgat
     8761 caccttcgac cgcgaaggat cgctgaatgg actgatccag acgggccagt taatcgacat
     8821 cgccgctgtg ccttgcaaca ggtgtgcagc aacctcatcg gcgttgaaac tcgcgtagcc
     8881 cggcggcgaa tatttaccga gctcgatcat gaattccaaa gctgcaattc cttcaggact
     8941 attgaccgca ggctttccat tcgcgtcaaa catctcaccg ccgaaggccc agaagatggg
     9001 catgaagtcg gcgacggcgg cgttgccctg cgcggcgcgc atgacgtaac cgtagacttt
     9061 cgatccgttc gcgcctgcgg tttctttctc gtggattgtt ttggctgccg cgagcacgtc
     9121 gtcccaggtt gcgggcgctt tcaaagaata tttctcaaaa agatctttgc gataaaaaaa
     9181 taattgcgag ttgccgacgt acggcagagc gaagagtgcg cccgtttgaa atggatggcg
     9241 gcagacgttc agcgacgctt cgacaaagtc ggaatccggg ccggattgcc cgcgtttttg
     9301 cagtagtggt gttagatccg taagaaaacg ctttcccgca aaccgcggaa accacgggtc
     9361 gtcgagcatg atgagatcgt aggcgccggt gtgagatttg agatctatta attctttctc
     9421 aaaaagattt gcgtaaggga attgagcgat ctcgatgtgt gtgccggtct tcgcctcgta
     9481 atcgcgggcg gcctgtttga gtgcctcgcc ttcggcacca gagatcaccg ccacgatcag
     9541 ttgtttttgc gatggttggc tggtgcagct tatggccagc agtaagagga gggtcaggag
     9601 tagtatgcgc atatttgtcg gcacagatta acactgatag cccggatcag aactgataat
     9661 atccgtctat gcgacaaatc gtaaccactg caaacggcga cattgacgtg ctcaaggtcc
     9721 aggagaagcc cgatccaaca ccgggcgacg acgaggtcgt gattcgcgtg cgcgctgccg
     9781 ggttgaactt cgcggacatt ctcgcgcgcc agggtttgta tcccgatgga ccgccgaaac
     9841 cgtgtgtgct gggttacgag gtcagcggcg tcgtcgacag tgtgggtaag aatgtgaacc
     9901 gatcgttcgt cggcaagtcg gtcgtggcac tgacgcgttt cggcgggcag tcagagatga
     9961 tcgcggtgaa ggcgacgcag atgtttgaga agcctgagca gttgacgttc gaacaggcgg
    10021 cggcgattcc ggtgaactac ctgaccgcgt atgcgttact cgtcgtcatg gggtcgctgc
    10081 acgagaacga gtctgtcttg atccacaatg cgggtggcgg tgttggactc gcagcgctcg
    10141 atatcgcgaa gaagatcggc gcggtgactt acggcacggc gagccagggg aagcacaagt
    10201 ttctaaccga ccgcgggtta gaccatccga ttgactatcg caaccaggac tggcagccgc
    10261 agttgatgca gctcacgaac ggccacggtg tagatctgat catcgatccg attggcggtg
    10321 tgcattggaa gaagagttac aacgcgttgc ggcacaccgg acgtttggga atgttcggcg
    10381 tttccgctgc ttcggcaaat gggatttcag gcaagctgaa aatgctgagg gcggtggttc
    10441 agatgccgcg gtttcatcct ctggggttac tgaacaagaa tcgcggcgtg ttcgggttga
    10501 acctgggcca catgtggcac gagcctgaga aggtcgcgtt gtggatgcgc gatattctgc
    10561 gcggggttga ggaagagtgg atcaacccgc acgtggacca ggcgttcttg tttgacgagg
    10621 tgggtaaagc gcaccggcgg ctcgaggagc gcaagaatat cggcaaggtc gtgctgattc
    10681 cttaaaaagg gtcaacatcc tgtaaatcct gaaatcatgt ctaacaagat gtcaggatta
    10741 acaagattca cgattcctct gatctgcgct tcgcaagaaa gaacgagagc actgtcgaat
    10801 agaccaagcc gaagagcatt gcgagcagga acaagccaat gaaggcgctg agggcgatgc
    10861 gcgaaatcgg cgcccgggtt gcgccgggct cactggcgag ccacttcgtt ccgacggtcg
    10921 cgaggacgac gacgaagaag atcgatgcgg acagtgcgta gacgaaagag atcgcgacgc
    10981 cggtcttcag tccttctttg aagtaggggc gatcgccttt cgcatgctcg agtgctttca
    11041 gtccgagata gatcatcgca aactggagga cgttgaaaaa gatcgggccg ccgagttggt
    11101 gaacgagtga ttgggggtta gggattagcg agtgtgtgat gagcacccac gccatcacac
    11161 cgagcgtgat cagaagtcca tacttaagag gtattttcat aatttttagc cgcggatttt
    11221 cgcggataaa cgcggattca ttcccaagct ggtttgtcgt cccagtaagg agcccacgcg
    11281 agtgtgagca cctttacggc gatgtttgct ttcgttggtt tgagactgac tttctcgagc
    11341 tgttctgttt gtggatcgag tgaccgttcg agtgctttgg tagcggcctg aaagtcagct
    11401 tccagattag aaagcgtctg tgaaatagct tcgacattgt cctgggcgcg ctcgacgtcc
    11461 tgcgattctt tcatcgaacg accaacaccg cgcgccgcag tcgtggcccg gcccagtgtc
    11521 gacatgctcg ttcgcttgcg acctaaaaac gacgacagca aggtcgcgcc aaacgagatc
    11581 gcggtctgca ttttctgtcc cttcgcctgt tccgactcgc gctcgaccgc ctgctccgca
    11641 cgacgctttc gatcctccaa cgcttcaaac ttcggcgcat acttccgtcg tagcttctcc
    11701 actgcctcat cacgttgctc acgcgcgagt tgctgtaggc gcacacgaaa gtcgcgctcg
    11761 ttctcccctg gattcgacac gatctccaac gtcgggctct cgagtaattc gagtcgctgg
    11821 ttgcgataaa cccagctcgc gagatccttg cgccagctcg cgtagctctt cgctttcgtc
    11881 gcttcagacg gcacttcacc gaactgcgct ccggcttccg gctccgattc gagatcttcg
    11941 acaggcagat cgctcgccgt agccttgccc cagtcgagac ccgctggtcc atccgcaaac
    12001 gccgcgagca aattcacctg ctgcgtcatc tcgaccgtct tcgaattgct gtaacgaact
    12061 tctgccgtgc cgagcagcat tggatgataa gtcagcgttt cgcccgcagc gccggatgag
    12121 cgaacgggaa tgtaatactg gctcacgtca ggcggtagta ttgctcgctc gcttttcggc
    12181 ggcgcactcg caaccggcgc aggcgcagaa accggggccg ccggcgccgc ggtcgctttc
    12241 aaaggatcca tcaacagctt gatctgcgcg cgcgtcagtg gtccacgcag gtatgacaac
    12301 gcccagcgcg tttcgaaaac ttctggcaca tcgtcgtgta cgttgttcag gaggaagatg
    12361 cgcttgtcga gtccggcgag cagttgctcc atcgcttgct tgtcaaactt ttgacccgtg
    12421 cccgcagcga tgccttcgag accttccagc acacgcgcct tgtcacgctc tgtttgcagc
    12481 cgaccgataa accacgtgcc ggtgtttgcg agtcctttgt aatcgagatc gaccggattc
    12541 tgcgtcgcga gcacgacccc gagtccgaac gcgcgtccct gtttcaacaa cgtcagcagc
    12601 ggtaacttcg acggcggatt tgcgacgggc ggaaagtagc cgaagatctc gtccatgtag
    12661 agaatcgcgc gcaggctggt cgtgcccgat tgcgttcgca tccagccaag cacctggttc
    12721 agcaacagcg acacgaagaa catgcgctct gcgtctgaga gatgcgcgat cgagaagatc
    12781 gagtgacgcg gcttgcctga ttcggtatga agaaacttcg cgacgtcgag cgactcgcct
    12841 tccatccagc tcgcaaacga cggcgacgcg agcaggtgat tcaacgacat cgccaactcg
    12901 aaacgcgcgt cagcagggaa gtacgaatcg agctccatca cgccgacttt cgtgatcggc
    12961 ggcgtctgca cgttttgaat tagactcgcg atgtcgagat cttttccggc agaccactcg
    13021 tggttcagga tgtttgaaag aagaatgtgc tcgcgactgc gaaccggatc ggcgttgatg
    13081 ccgatcagcg cgagcaaact cgtaacggta gtgttaatgc gctcggcaag tgcttcgttg
    13141 tcttgcctga tcgtttcagg tggcgcgacg aatgatttga gaatcgagac aggcagtccg
    13201 gcattgctgc ccggcgtgta gatgcggaag tcagccgcgt cgcgcaggcg tttgatgcgc
    13261 tcgccgtctt cgccccactt cgcgagtcct tctttccaga gttgtgcctg ttgtgcggcg
    13321 tcgacgcctt cctcgaccca cggcgcgaag tcttccggcg cgaggttcgg aaa
//
LOCUS       DASSFQ010000136         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1828227, whole genome shotgun sequence.
ACCESSION   DASSFQ010000136 DASSFQ010000000
VERSION     DASSFQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828227"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFH96_04720"
     CDS             complement(<1..383)
                     /locus_tag="VFH96_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973302.1"
                     /translation="MENKTLNQKTLLIFAPLLILTGIAGFVIPAQYSLMSGAAPYNLF
                     HIIFGSIGLLITMTKNDLLASSFNLGFGLIDLYQVLASVVGLAPIQYFHWTYADDVLH
                     VLIGFALVIIGGRGLTRIWGRFSAE"
     gene            complement(373..1680)
                     /locus_tag="VFH96_04725"
     CDS             complement(373..1680)
                     /locus_tag="VFH96_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET6973303.1"
                     /translation="MHSLSTALSPQEDKLLACIHCGLCLEACPTYVVTGNENDGPRGR
                     LYLMRAVAEGRLEETSSAFATHIDRCLGCRACEQACPAGVQYGQLLEASRELLLDTQP
                     KSDLANKLLRFVLRHVWQSPTRLRLFFRASRVFRDLGLARLLLKSRLARLISPRAEFG
                     LALLENSRGISRAKAQRRKGRNRDNNLLMFAGCVGEGLFTGVNRATERVLRANNFGVN
                     HPPEQVCCGALHAHAGDLEGARKLARQNIAAFNSDDPIVTNAGGCGAMLTTYGHLFAN
                     DQDLAQQAAHFSARVRDVSQQLATTDMRTAPSPVDVPVTYDTSCHLLYGQRGGETSER
                     MLRAVTGDNFVALNGTERCCGAAGVYNLLQPEMSQLVLGEKLDHINSTHAAILATGNP
                     GCQMHIGAGACLAHMELKVCHPIELVDQAYERAGFYEDLTDGK"
     gene            complement(1724..>2199)
                     /locus_tag="VFH96_04730"
     CDS             complement(1724..>2199)
                     /locus_tag="VFH96_04730"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET6973304.1"
                     /translation="LNDEMDDFAAKPGTPNMYGLIQGERNAVAAKKRPLSAMTPTIVL
                     HKDGSLWFTVGSPGGPTIINTVLCIITNVVDYEMNIQQAIDFPRIHHQWLPDELVGEP
                     FGLSGDTQHALTARGHTLGKLRYLGDAEGIMIEEKTGMRLGATDPRRSDGQAVGY"
BASE COUNT          474 a          635 c          607 g          483 t
ORIGIN      
        1 tactcggcgg aaaatcggcc ccagatccgc gttaatccgc gtcccccgat gatcaccagc
       61 gcgaaaccga tcaggacgtg cagcacgtcg tccgcataag tccagtggaa gtattgaatc
      121 ggcgctaaac cgacgacact cgccagcacc tggtatagat cgatcaaccc aaatcccagg
      181 ttgaacgagc tggccagcag gtcgtttttg gtcatcgtga tcaacagccc gatggagccg
      241 aagatgatgt gaaagaggtt gtaaggagcg gcgccgctca tcaggctgta ctgtgccggt
      301 atcacaaaac ctgcgatacc cgtcagtatc agcaacggcg cgaagatcag cagcgttttc
      361 tgattgaggg tcttattttc catcggtcag atcctcgtag aagccggcgc gttcgtaggc
      421 ctggtccaca agctcgatag gatggcagac tttcaattcc atatgcgcga ggcaggcgcc
      481 tgcgccgatg tgcatctggc agccgggatt gccggtggca agtatagcag cgtgcgtcga
      541 gtttatgtgg tccagtttct cgccgagtac gagctgcgac atttccggct gtaacaggtt
      601 gtatacaccg gccgcgccgc agcatcgttc ggtcccattt agcgcgacaa aattatcacc
      661 ggtgactgcg cggagcattc gttcggaagt ctcaccgccg cgttggccat ataaaagatg
      721 acaggaggtg tcgtacgtta cgggaacgtc gacagggctg ggagcggtgc gcatatctgt
      781 agtagcgagt tgctggctga cgtcgcgcac gcgagcactg aagtgagctg cctgttgggc
      841 aaggtcttga tcattcgcaa acaaatgccc gtacgtggta agcattgcgc cacacccgcc
      901 ggcgttagtg acgatcgggt catctgagtt gaacgcagcg atgttttgcc gagccagttt
      961 gcgtgcgcct tcgagatcgc ctgcgtgagc atgcaaggcg ccgcagcaaa cctgttcggg
     1021 cgggtggtta acgccgaagt tgttcgcccg aagcacacgc tcagtcgcac gattcacacc
     1081 cgtgaacagt ccctcaccaa cacacccggc aaacatcaat aggttattat ctctatttct
     1141 tcctttgcgc ctttgcgcct ttgcgcgaga aatccctctc gagttttcca gcagcgccag
     1201 cccaaactca gcccgcggcg aaatcagtcg cgctaaacgt gacttcaata acagccgcgc
     1261 caatccaaga tcgcgaaaga ctcgcgatgc tctgaagaac aaccgcagtc tcgttggtga
     1321 ttgccagacg tggcgcaata cgaaccgtag cagcttgttg gctaaatccg atttcggttg
     1381 cgtgtcgagc agaagctcgc gactcgcttc cagtaattgt ccgtattgca caccggccgg
     1441 gcaggcctgt tcacacgcgc ggcagccgag acagcgatcg atgtgtgtgg caaacgcgct
     1501 tgaagtttct tccaaccgac cttccgctac ggcccgcatg aggtagagcc gcccacgtgg
     1561 cccatcgttc tcgttaccag tgacgacgta cgtcggacac gcctcgagac acagaccgca
     1621 gtggatgcat gccagtagtt tgtcttcctg cggtgataag gcggtcgaga ggctatgcat
     1681 tatgcttcac aggatttaca agatgttcca ggattatcag taatcaatac ccgacagcct
     1741 gtccatcact ccgccgtgga tcagtcgcgc ccaatctcat tcctgttttc tcttcgatca
     1801 tgatcccctc agcatcgccg agataacgca gcttgccgag cgtgtgcccg cgcgctgtga
     1861 gcgcgtgctg cgtgtcgccg gataacccga atggttcacc cacgagttcg tccggcaacc
     1921 actgatgatg aatacgcgga aagtcgatcg cctgctgaat attcatctcg tagtcgacga
     1981 cattcgtgat gatgcagagc acggtgttga tgatcgttgg accaccggga cttcccaccg
     2041 tgaaccacaa actaccgtct ttgtgcagca cgatcgttgg agtcattgcc gacagcggac
     2101 gctttttcgc cgccactgca ttccgctcac cctggatcag tccgtacatg tttggcgtgc
     2161 ccggcttggc ggcgaaatcg tccatctcgt cgttgagaa
//
LOCUS       DASSFQ010000137        14755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1092816, whole genome shotgun sequence.
ACCESSION   DASSFQ010000137 DASSFQ010000000
VERSION     DASSFQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14755
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092816"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VFH96_04735"
     CDS             <1..1329
                     /locus_tag="VFH96_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973305.1"
                     /translation="QLDLSQTFTEYNYDSDKNVLRCGVSHALGDWAGQNNTNGIYLPM
                     GMCAHVHLGGHVHTGGWGIVARSHGILADHVLAFDIILANGQNEHIVRPEAGKTTQHN
                     DDVYYAVLGGGRGGDFGIVTHWEFRPIRDEYPPKSMCYSFVWLWSKEKMEAVVRRMEE
                     LTKLCADKTVPSDYEFMLTITGYGWTNLFSDEMKTAITMRAVRDGMRAHDGISLPLPP
                     LIQMWMCYTHKDPNKQFDDQWFESFVSAVGDPLVAHRQNMSVSEGITKYFIMRQDREM
                     NYPFVKRARVTMDLPAGFAEKYTERMYEIMSFPTSLCQHLVSQMQIYAGGAVAENDKA
                     GITAYSWRNQAFAMGHDDFYSDDWFCGDERSNAEQWQQQNDEVFIGPKGAFADADMRM
                     FAYTFGDRVLDKVWPYFYDSKEKYDRLRRIKGELDPDGLFSADEFSLKPL"
     gene            1458..2465
                     /gene="hemE"
                     /locus_tag="VFH96_04740"
     CDS             1458..2465
                     /gene="hemE"
                     /locus_tag="VFH96_04740"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET6973306.1"
                     /translation="MRACRCEPTPYTPVWLMRQAGRYMREYREVREKTTFLELCKNPA
                     LAAEVTVTAVERLGVDAAIIFADILLILEPLGIELEFAKGEGPVIHNPVRRPADVDRL
                     RELQDPNELDFVYEAIRQTRSALKPDIPLIGFCGAPFTLASYMTEGGGSKNYVHTKQL
                     MYDDAGAWHAMMKMISRALVKYLNAQIEAGAQAVQLFDSWVGSLSPDDYREFVLPHTQ
                     SVIRGVTKGVPVIHFGTGTAALLELMREAGGDVIGLDWRVRLDEGWRRVGHDVAVMGN
                     LDPVVLFAKRDVLREQAKRILDQAEGRPGHIFNLGHGILPETPVENVVALVEMVHGLN
                     G"
     gene            2462..3424
                     /locus_tag="VFH96_04745"
     CDS             2462..3424
                     /locus_tag="VFH96_04745"
                     /inference="COORDINATES: protein motif:HMM:NF000689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET6973307.1"
                     /translation="MKTDFDALLLVSFGGPEGMDDVIPFLENVLRGRNVPRERMLSVA
                     KHYELFGGVSPINQQNRNLIVALEKELERHGPRFPIYWGNRNWHPLLPDTLRQMADDG
                     IKNALAFVTSAYSSYSSCRQYLQNISDAQAAVGPEAPRVEKLRVFYKHPLFIEANVDH
                     IRVALDRLGEPAHLVFTAHSIPESMAVNCDYASQLGHTGALIARELPIEKWHMVYQSR
                     SGSPMQPWLGPDVCEHLRTLRNEGVTNVVVAPIGFVSDHMEVVYDLDVEARRVADEIG
                     MKMVRAQTAGTHPAFIRMIRELIMERINGEAPMNICAPDCCRMS"
     gene            3435..4841
                     /gene="hemG"
                     /locus_tag="VFH96_04750"
     CDS             3435..4841
                     /gene="hemG"
                     /locus_tag="VFH96_04750"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET6973308.1"
                     /translation="MIDRIVIIGGGISGLAAAHRVLELNQAAQVTILEASDRLGGTIQ
                     TEYRDGFLIERGPDSFISEKPHAIALAKRLGIESQLIQTNEEYRRSFIVRNGRLRAVP
                     EGFQLMAPSRMWPFIASDIFSIPGKARMAADLLLPRKSANGSSDESLASFVRRRLGEE
                     ALVRMAQPMVGGIYTADPETLSLRATLPRFLDMEQKHRSLILAMLRQGRAQKTGTSGA
                     RYSLFVSFERGMQVLVDAITQVTRINADLRVKTSAQRLSFDHGWTITTDKDEQIKADA
                     VCLAVPAYVAASLLSDRDEGLAQKLRQIKYASTATINFGYRREAIAHPLNGFGFVVPF
                     VEKRSLIACTFSSVKFSGRAPDGQVLLRAFAGGALQPEIFALDEQEISRRVEADLREL
                     LGIREDPRFIETAKWERSMPQYEVGHLDRVSEIEKLASALPGLSLAGNAYRGAGIPDC
                     VRSGEAAAEYLVNHRASA"
     gene            complement(4823..7210)
                     /locus_tag="VFH96_04755"
     CDS             complement(4823..7210)
                     /locus_tag="VFH96_04755"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET6973309.1"
                     /translation="MNVVTERLGVSDRSVYILIGLCFVLSGATGLIYEVLWARMLGLV
                     FGATTLAVSTVLAAFMGGLALGSALAGKLAQRIRKPLSTYGVMEIGIAVYALLVPLLF
                     RWVDHLYALIWQQLHPGYFTFSLWRFLLSGLVLLVPTTLMGATLPVLTVAFARSSDSN
                     SVTKLYACNLAGAILGTLAAGFVLLPALGVRMTIAVAAVLNVIVGVIAIVLQRRTKAH
                     SEFSSHPVYPENPANPVNSFWYFAALASGFVTISSQVSWTRTLTMIIGSSTYAFSIVV
                     ALFLIGLALGAWLVTRKDRSQRLRSAILKVEALTALSLLLSLYVLNRIPALMINLGLG
                     LHISSWAGLLALQIVSATLLILLPAVLMGMVMPLVLVWASSDRARAVAQVGRSYAVNT
                     IGAIAGAFITGFILIPKTSTRFTLLFAAICCLVVATVAYNPVGRDPALKRSLAVGLIP
                     VVVIVLFIIAPPMNLADLSIGAYDSLVRVLAQTREGVKEAETGPNIHELLMYEEGPTA
                     TVSVRRDQNTVSMAINGRTNASDSNYDMPTQVMLGQLPLLIAPKIDHGLIIGYATGVT
                     VGAMLQSPVQSVTCVELEPGTVAGSQFFNHVNNRPLDDPRVNLIIDDARTFLRVTPNR
                     YDMIVSEPSHPWVPGVANLFTQEFFELGRSRLSDNGIWVQWVQIYQLSTESLRSVLAT
                     YQRVFPHVLVFRVGGLSNGKDLLLIGSNQPLNLDHLPERFRDPRMAAELARVDLKSEA
                     DIRGWYVCDESKLGPAVAGAKINTDDNMHIEMTVPREAFRPLMQSNADWIQALAR"
     gene            complement(7236..8561)
                     /locus_tag="VFH96_04760"
     CDS             complement(7236..8561)
                     /locus_tag="VFH96_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6973310.1"
                     /translation="MKPEVKIDRAAPQKTSMLRVILGTLLVLAGLTSALVTVLARSNG
                     DNSLAGTAAILSLVIALLLTIFIVPPLARSARVEVANIDFPFEPTSGGGVFLVIIIVV
                     GFAAWNTGNNLLFLVFSLLCSTLFVGWIHAGTSLRDLTVSARFPDHIFAAEAAPVIVT
                     LRNTKRLLPSFSILVEARGPVNEVDPKRRRRHLKRLLAYFSYVPHRAAAEQRVEQLFP
                     TRGHVLIDGFELSTRFPFGFFRRRRRLRARNVDIIVYPKPEVISDKLHLLPMYAGRMP
                     AMRRGAGHDLFSMRDYQPQDDLRHIDWKATARSRRLTVREFTSEDERRITIVLDTRLS
                     HGAGNDLPERFERGVVQAASLIKHFIDERAEVRLVLGDDVGPFGSGAEQLYRCLRRLA
                     LVIPSDREGPGLANVSWTAVEHDYAILLTAAAPGSIPANIWRTSHVIYL"
     gene            complement(8558..9541)
                     /locus_tag="VFH96_04765"
     CDS             complement(8558..9541)
                     /locus_tag="VFH96_04765"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6973311.1"
                     /translation="MRNLESISSEQTASANAVEQIERLQQAVETVIKGKPEAIRLAIV
                     TLIAGGHLLVEDVPGVGKTTLAQALARALDCSFQRIQFTSDLLPSDVIGLSVFNQHSG
                     LFDWKPGPVFANVVLADEINRTTPKTQSALLEAMAERHVTVEGMTHDLPAPFIVLATQ
                     NPIEHHGTYPLPESQLDRFMLRLRIGYPAPADEQKILRDREFRDPLNELEPVMSAREV
                     VELQQTVASVAVDDVLVQYLMRIVAATRSSEMLDLGVSPRGTLSLFRAAQALALTEDR
                     TYCIPDDIKRLVVPVFAHRIVVSSRFSSSLRRSEEAEAVLNEILKTVSVPL"
     gene            9719..10204
                     /locus_tag="VFH96_04770"
     CDS             9719..10204
                     /locus_tag="VFH96_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6973312.1"
                     /translation="MEPLNNSKKNQRGFSLIELMIVIAIIGILIGVGIAGYKAAIRAT
                     NEAAAVKTLRSIAEQQMLYFNSHQRNSFGTFEEMRKENLLDSRFDGSTPVVDGYVYTM
                     KVIPKSTSAQPGYTINADPQVPTGVSATGKNFYYVDSDTNTIHVNANQQATATDPPIG
                     Q"
     gene            complement(10272..11882)
                     /locus_tag="VFH96_04775"
     CDS             complement(10272..11882)
                     /locus_tag="VFH96_04775"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HET6973313.1"
                     /translation="MIRPFTLSLLILALASSAFTSGHTVHSTATIAVAQKASAFDVRK
                     YGAKGDGKALDSPAINKAIDAAAAAGGGTVLFTAGTYRAFSIRLKSNVALYLDQGATI
                     LAADPRDGDGKYDLPEPNQWDQYQDFGHSHWHNSLIWGENLENISILGPGRIWGKGLV
                     RSGSQSRTKEQNDALEKQGPDPKAGPFGYPNARDAVEPGWGNKAISLKLSRNVIIRDI
                     SILHGGHFAILATGVDNLTIDNVKIDTNRDGIDVDACKNVRVSNCTVNSPFDDGICPK
                     SSYALGYARVTENVTITNCQVSGYDEGTLLDGTYKRDFRNANGTFSPTGRIKFGTESN
                     GGFKNITVSNCVFDYSRGLALEAVDGALLEDVTISNITMRDISNSPIFLRLGARNRGP
                     KETTKTGALRRVIISNVVVYNADPKYSSIISGIPGFLIEDVQLNNIRIYAKGGGTKEQ
                     AALEPPEREALYPEPTMFGELPAYGFFIRHVKGLQMRDVEVSYLSPDLRPAFWLNDVT
                     DVEFVHVKTQREAAPDQRIERLEGKKTF"
     gene            11911..14364
                     /locus_tag="VFH96_04780"
     CDS             11911..14364
                     /locus_tag="VFH96_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6973314.1"
                     /translation="MRETLLSFLDDCRAHGEQTAVTHEAGARPSRWSYAHIASSASQF
                     ARELETRGVSTGDRVLFWGESRPEWIACFYGCLLRGAVVVPLDLKSVPDFVVRVQQQV
                     SAKLLLADEPQLDIPHLTFNSLTDLLARHPDQPYSTKAKPDDLVQIVFTSGTTAEPKG
                     VCLTHRNLLANIAPIEKEFNRYRRWEKFVHPLRFLCLLPLSHVFGQMMGIFIPQLLGA
                     EVYFSESYKPSQIIRSVKKHRINAVVTVPRVLETLREKVKAELTDDQIRAARGRHFLR
                     NWWAFRKIHRQFGWRFWAFITGGATLESETETFWRRLGYAVIQGYGMTETASLTSLSH
                     PFRMRHGSIGKPVAGQEVKLSDDGEILVRGENVSHGYWNGNGQSLTNDEGWIHTGDIG
                     EFGPGGNIYFRGRSKETIVTAAGLKIYPTDLEAALDRQPEIKASAVIPLTGGSEALAV
                     LIPHEEADLSAAVQRANESLAEFQRIRHWIAWPNSDFPRTPGTRKVIKGQIAQAVQTM
                     LQPSTSAAADKDLAPLSLPVISRITRVEPAALSPNANLADDLKLDSLGRVELLSALED
                     QYQIELDEAAITEATTIADIERIVSRGKSEAVAYPYPRWAMRFPFTWIRFVVYHVFFL
                     PLTLIMCRVRTIGVERFAKVKPPVLFISNHVTDVDAALILSALPWRWRYRMAIAMAGE
                     ILREWRMNAKVQYALGAALFNVFSLPRQSGFRQSFEYAGEAVDRGFSILIFPEGTETK
                     DGQLQPFKAGIGLITSELKVPVVPVMLRGLFEVKKRKQRFVKPGTVSITFGDPVEFSS
                     DQTPSDITRDLELRLQSVQ"
     gene            complement(14381..>14755)
                     /locus_tag="VFH96_04785"
     CDS             complement(14381..>14755)
                     /locus_tag="VFH96_04785"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6973315.1"
                     /translation="VFGAPLSDGRDCANALDAAQEILARVRDEVEAGTILPTTVGIGL
                     HAGEAVTGSIGSALRREYTVIGDVVNLAARIEKLNKRFDSQLLISEMVWQAVSSDRSK
                     VVPMGEVEVRGREQAIQVYQVA"
BASE COUNT         3378 a         3991 c         4231 g         3155 t
ORIGIN      
        1 cagctcgact tgagccagac ttttacggag tacaattatg attctgataa gaacgtgctg
       61 cgctgcggag tcagccacgc cttaggtgat tgggccgggc aaaacaacac gaacgggatc
      121 tacctgccga tgggaatgtg cgcacacgtg cacctcggcg gacatgtaca caccggcggc
      181 tggggaatag tggcgcgcag ccatggcatc ctcgccgatc acgtacttgc cttcgacatc
      241 attctcgcca acggccaaaa tgaacacatc gtgcggccgg aagcaggcaa gacaacgcag
      301 cacaacgacg atgtctatta cgccgtgttg ggaggagggc gtggaggaga cttcgggatc
      361 gtgacgcatt gggaattccg tccaatacgc gacgagtatc cccctaaatc catgtgctac
      421 tcgttcgtgt ggctttggtc gaaggagaag atggaagcag tggtgcgccg gatggaagaa
      481 ctgaccaaac tgtgcgcaga caaaacagtt ccatccgact acgaatttat gctaaccatc
      541 acgggttacg ggtggacgaa cctgttctcc gatgagatga agactgcgat aactatgcgg
      601 gcagtccgcg acggcatgcg agcacacgac ggtatttcgc ttccccttcc gcctctgatc
      661 cagatgtgga tgtgttatac gcacaaagac ccgaacaaac agttcgacga ccagtggttt
      721 gagtcgttcg tatcggctgt tggagatcca ctggtggccc atcgacagaa catgtctgtc
      781 tccgaaggaa tcacgaagta tttcattatg aggcaggatc gcgagatgaa ttatccgttc
      841 gtgaagcgtg ctcgagtgac gatggatcta cccgctggat ttgccgagaa gtataccgag
      901 cgtatgtacg agatcatgtc cttccccacg tctctctgcc agcacctcgt ctcgcaaatg
      961 cagatctacg cgggtggcgc agtggcggag aacgacaaag cgggaatcac ggcgtattcg
     1021 tggcgcaatc aagcttttgc tatgggccac gacgatttct attcggatga ctggttctgc
     1081 ggcgatgagc gctcgaatgc ggagcagtgg cagcagcaga atgacgaggt attcatcggg
     1141 ccaaaagggg ctttcgccga cgcggatatg aggatgtttg catacacctt tggtgaccgc
     1201 gtgttggaca aagtgtggcc ctacttctac gacagcaagg aaaagtatga ccgattgcga
     1261 agaatcaaag gggagcttga tccggatggc ctcttctctg ccgatgaatt ttcgttgaag
     1321 cccttgtaga actcagatca ttttagccgc ggatttccgc ggatgaacgc cgatctgatc
     1381 cgcgtcaatc cgcgaaaatc cgcggcttac tatataattc tcttcctttg actcaatcac
     1441 tacacgacag tccgtttatg cgtgcgtgcc ggtgcgagcc cacgccttat acgcctgtct
     1501 ggctgatgag gcaggcgggg cgctatatgc gcgagtatcg ggaggtgcgg gagaagacga
     1561 cgtttctcga gctttgtaag aatccggcgc tggcggcgga ggtgacggtt acggcggtgg
     1621 agaggttggg ggttgatgcg gcgattatct ttgccgatat tctgttgata ctcgagccgc
     1681 tcgggatcga gttggagttt gcgaagggtg aggggccggt gattcataat ccggtgcgaa
     1741 ggcctgcgga tgtggatcga ctgcgagagc ttcaggatcc gaacgaactc gattttgttt
     1801 atgaggcgat caggcagacg cggagtgcgc tcaagccgga tattccgttg attggttttt
     1861 gtggggcgcc gtttacgctc gcatcatata tgactgaggg tggtgggtcg aagaattatg
     1921 tccacacgaa gcaactgatg tacgacgacg cgggtgcgtg gcacgcgatg atgaagatga
     1981 tttcgagagc gctcgtgaag tatctcaacg cacagatcga ggcaggcgcg caggcagtgc
     2041 agttgtttga ttcgtgggtc ggatctttga gccctgatga ctatcgcgag tttgtgttgc
     2101 cgcatactca gagcgtgatt cgtggggtca caaaaggcgt tccggtaatt cattttggga
     2161 cggggacggc ggcgctactg gagttgatgc gcgaggccgg tggcgatgtg atcgggttgg
     2221 attggcgtgt gcgattggat gaaggctggc ggcgcgtggg ccatgatgtg gcggtgatgg
     2281 ggaatctcga tccggttgtg ttgtttgcga agcgcgacgt gctgcgggag caggcgaagc
     2341 ggatactcga tcaggcggaa ggacgaccgg gacatatttt caatttgggg catgggattc
     2401 tgccggagac accggtggag aatgtggtgg cgctcgtgga gatggtacac ggattaaacg
     2461 gatgaagacg gattttgatg cgctgctgtt agtgtcgttt ggtgggccgg aggggatgga
     2521 tgatgtgatt ccgttcctcg agaatgttct gcgtggaaga aacgtgccgc gggagcggat
     2581 gctcagcgtc gcgaagcatt atgagttgtt tgggggcgtg agtccgatca atcagcagaa
     2641 tcgaaatttg attgtggcgc tggaaaaaga attagagcgg catgggccaa ggttcccgat
     2701 ctactggggt aatcgcaact ggcacccgct tttgcctgac acgctgcggc aaatggcgga
     2761 cgatggaatc aagaatgcgc tggcgtttgt gacctctgcg tatagctcgt attcgagttg
     2821 ccggcagtat cttcagaaca ttagtgacgc gcaggccgcg gttggaccag aggcgccacg
     2881 cgttgaaaag ttgcgagtgt tctataagca cccgctgttt attgaagcga atgtggatca
     2941 tattcgggtc gcgttggatc gactaggaga gccagcgcat cttgtattta cggcgcacag
     3001 tattccggag tcgatggcgg tgaactgtga ttatgcgtcg cagctcgggc acacgggcgc
     3061 tttgattgcg cgtgagcttc cgattgagaa gtggcatatg gtttatcaga gccgcagtgg
     3121 atcgccgatg cagccgtggc ttggaccgga tgtttgtgag cacttgcgaa cgttgcgtaa
     3181 tgaaggtgtg actaacgttg ttgtggcccc gatcgggttt gtttcggatc atatggaagt
     3241 tgtttacgat ctggacgtcg aggcacggcg ggtggcggat gagattggga tgaagatggt
     3301 gcgcgcgcag acggcgggaa cgcatccggc atttatcagg atgatccgcg agctgattat
     3361 ggaaagaatt aatggtgagg cgccaatgaa tatctgcgcg cctgattgct gtcgtatgtc
     3421 ttaaacagga ttacatgatt gacaggattg tcataatcgg tggcggtatt tccggtctcg
     3481 cggcggcgca tcgtgttctc gaactcaatc aagcagcaca agtcacgatc ctcgaagcgt
     3541 cagatcgact cggtggcacg attcagactg agtatcgcga cggtttcctc atcgaacgtg
     3601 gtccggattc tttcatctcg gaaaaacctc acgcaatcgc actcgctaaa cgattaggga
     3661 tcgagtcgca actaatccag acaaacgaag aataccggcg gagctttatc gttcgaaatg
     3721 gacgcctgcg cgccgtgccc gaagggtttc agttgatggc gccgtcgcgt atgtggccgt
     3781 tcatcgcgag cgatattttc agtattcccg gcaaggcgcg catggctgct gaccttttgc
     3841 tgccgcgtaa aagcgccaac ggttcaagtg atgagagtct cgcgagcttt gtgaggcgca
     3901 ggctcggtga agaggcgctc gtgcgcatgg cccagccgat ggtggggggt atttacactg
     3961 ccgatccgga aacgctgagc ctgcgcgcca cgctgccgcg gttcctcgat atggaacaga
     4021 agcatcgcag tctgattctc gcgatgttgc ggcaaggtcg tgcgcagaaa acggggacca
     4081 gcggcgcgcg ttacagtttg tttgtgtcgt tcgagcgagg gatgcaggtg cttgttgacg
     4141 cgataacaca ggtaacacgg attaacgcgg atcttcgcgt gaagacgagc gcgcaaagac
     4201 tcagcttcga tcatgggtgg acgattacca cggataaaga cgagcagatc aaagcggatg
     4261 cggtgtgttt ggcggtgccg gcgtatgttg ctgcgtcgct gttgagcgat agggatgaag
     4321 gacttgcgca gaagctcaga cagattaaat atgcgtcgac cgcgacgatt aattttggtt
     4381 acaggcgcga ggcgatcgcg catccgttga atgggtttgg gtttgtggtg ccgttcgttg
     4441 aaaagagatc gttgattgcc tgtacgttta gtagcgtgaa gttttcgggg cgcgcaccgg
     4501 atgggcaggt gctgttgcgc gcgtttgcgg gcggggcgtt gcaaccggag atcttcgcac
     4561 ttgatgaaca ggagatctct agaagagttg aagctgatct gcgtgaatta ttaggaatcc
     4621 gcgaagatcc gcgttttatt gaaacggcga agtgggaacg ctcaatgcct caatacgaag
     4681 tgggccatct cgatcgagtt agtgagattg aaaagctcgc cagcgcactg ccagggttga
     4741 gtttagctgg taacgcgtat cgcggtgctg gaattccgga ttgtgttcgc agcggtgaag
     4801 cggctgcgga gtatctggta aatcaccgcg caagtgcctg aatccagtcg gcgttgcttt
     4861 gcattaatgg tcgaaaggct tcacgcggca cggtcatttc gatgtgcatg ttgtcatcag
     4921 tattgatctt cgcgcccgcg actgccggac caagcttcga ctcatcgcaa acgtaccacc
     4981 ctcgaatatc agcttccgac ttcaaatcaa cacgcgccag ctcagccgcc atgcgcggat
     5041 cacggaagcg ttctggtaag tgatccagat tcaaaggctg gttacttcca atcagcaata
     5101 aatccttgcc gttgctcaaa ccacccacgc gaaacaccag cacgtgcggg aagactcttt
     5161 gatatgtcgc gagtacggaa cgcaaactct cggttgataa ttgatagatc tgcacccact
     5221 gcacccaaat cccattgtca ctcagccttg agcgcccgag ctcaaaaaat tcctgcgtga
     5281 acaagttcgc cacgcccggc acccacggat ggctcggctc actcacgatc atgtcataac
     5341 gattgggcgt cacgcgtaga aacgtcctcg catcgtcgat gatcaaattt actcgtggat
     5401 cgtcgagcgg ccgattgttc acgtgattga aaaactgcga acccgccacc gtcccgggct
     5461 cgagctcgac acacgtgact gactgcacgg gactctgcag catcgcgccg actgtcacgc
     5521 cggtcgcgta accaataatc aatccgtggt cgatcttcgg cgcgattagc aacggaagct
     5581 gcccgagcat cacctgcgtc ggcatgtcgt agttactgtc agacgcgttc gtgcgaccgt
     5641 tgatcgccat cgagaccgtg ttttgatctc gacgaacaga aaccgttgcc gtcggacctt
     5701 cctcgtacat cagcagctcg tggatgtttg gtccagtctc agcttctttc acaccttcgc
     5761 gagtttgggc caacacacgc accagactgt cgtacgcgcc aatcgagaga tctgcgagat
     5821 tcatcggcgg cgcgatgatg aaaagcacga tcacgacgac tggtatcaaa ccaacagcga
     5881 ggctgcgctt caacgccgga tcgcgaccaa cggggttata tgcgaccgtg gcgacgacga
     5941 gacaacagat cgccgcaaac aacaacgtga atcgcgtgct tgtcttggga attaggatga
     6001 aaccggtgat gaacgctccg gcaattgctc caatcgtatt caccgcataa ctgcgtccga
     6061 cctgcgcgac tgccctcgcc cgatcgctac tggcccaaac cagcacgagc ggcatcacca
     6121 tacccatcaa cacggcaggc aacagtatta gcaacgtcgc tgaaacgatc tgaagcgcaa
     6181 gcagaccagc ccatgaagaa atgtgcaaac ccagaccaag attgatcatg agcgccggaa
     6241 ttctgttcag cacgtacaga ctcagcaaca gactgagcgc tgtcagtgct tcgactttca
     6301 ggatcgcgga acgcaaccgt tgtgagcgat cttttcgtgt aaccagccag gcgccgaggg
     6361 cgagaccgat caggaatagt gcgacgacga tgctgaatgc gtacgtgctg gagccgatga
     6421 tcatcgtgag cgtgcgcgtc catgagactt gtgagctgat ggtcacgaag cccgatgcga
     6481 gcgcagcgaa gtaccaaaag ctgttgacag gatttgcagg attttcagga tagacaggat
     6541 gtgaactgaa ttccgagtgc gcctttgttc gacgctgcaa gacgattgcg atcacgccga
     6601 cgatgacgtt cagcactgcc gcaacagcga ttgtcatgcg aacgccgagc gcgggcagca
     6661 acacaaaacc ggcggcgagt gttccgagga tcgcacccgc gagattgcag gcgtaaagct
     6721 tcgtgacaga gttcgaatct gacgaacgcg cgaaagctac agtgaggact ggaagcgttg
     6781 cgcccatcaa cgtcgtgggc acaagcaaca cgagtccgga aagcaggaag cgccaaagac
     6841 tgaaggtgaa gtatccgggg tgaagctgct gccagatcag tgcgtacaag tgatcgaccc
     6901 agcgaaacag aagcggaacg agcagcgcgt aaacagcgat gccgatctcc atcacaccat
     6961 acgtgctcag cggctttctg attcgctgtg cgagcttccc tgccaacgcg ctgccgagcg
     7021 cgagcccgcc cataaacgcc gcgagcacag tgctgaccgc aagcgtagtc gcaccaaaaa
     7081 ccagaccgag catccgcgcc cagaggactt cgtagatcag tcctgtcgcg ccggaaagta
     7141 caaagcacaa accgatcaga atgtagacag atcggtcgga gactcctaaa cgctcagtaa
     7201 caacattcat tttgtgtttt tgtgcctttt tgtggctaca agtagatcac gtggctcgtg
     7261 cgccagatgt ttgcaggtat cgatcccggc gccgcggcag tcaacaaaat cgcgtaatcg
     7321 tgctcaaccg cggtccagct aacattcgca agccccggcc cttccctatc cgacggaatc
     7381 accagagcca gccggcgcaa gcagcgatac agttgctccg ctcccgaacc gaatggtcca
     7441 acatcgtccc ctaacaccag cctgacctcc gcgcgctcgt cgatgaagtg tttgatcagc
     7501 gaagccgcct ggaccacacc acgctcaaac cgctccggaa gatcattacc cgcaccatgc
     7561 gaaagccgcg tatcaagcac aatggtaatg cgacgctcat cttcggaagt aaactcgcgc
     7621 acagtcaatc gtctcgatct cgcggtggct ttccaatcaa tgtgacgcaa gtcatcctgc
     7681 ggctggtagt cgcgcatgga aaacagatcg tgtccggcgc cacgccgcat cgccggcatt
     7741 cgacccgcat acatcggcaa caagtgtaac ttgtcgctga tcacttccgg cttcggatac
     7801 acgatgatat ccacgttccg ggcgcgcaaa cgacgtcgcc gtcgaaagaa accaaacgga
     7861 aaacgagtcg agagctcaaa cccatcgatc agcacgtgcc cgcgcgtcgg aaacagttgc
     7921 tcgactcgct gttccgcagc cgcccggtgt ggcacgtagc taaagtaggc gagtaaacgt
     7981 ttgaggtgac gccgacgccg tttcggatcc acttcgttaa ctggaccacg cgcctcaacc
     8041 aggatagaaa atgacggcag taaacgcttc gtgttccgca gcgtcacgat cacgggcgcc
     8101 gcctctgctg caaagatgtg atccggaaaa cgcgccgaaa cagtcaggtc gcgcaacgac
     8161 gtgcccgcat gtatccagcc gacaaagagc gtcgaacaca acaacgaaaa cacgagaaac
     8221 agcaagttgt ttccggtatt ccaagccgca aacccgacca cgatgatgat caccagaaac
     8281 acaccaccac cactcgtcgg ctcgaaagga aaatcgatgt ttgcgacttc aactcgcgcc
     8341 gaacgcgcca ggggcggcac gataaaaatc gtgagcagca acgcgatcac gagcgacaag
     8401 atcgcagccg taccagcaag actattgtca ccattgctac gcgccagcac cgtcacgagc
     8461 gcagacgtca gcccggcgag aaccagcagc gtcccgagaa tcacgcgcaa cattgacgtc
     8521 ttctgaggag ccgcgcgatc gatctttacc tcaggcttca cagcggaaca ctaacagttt
     8581 tcaggatttc gttaaggacg gcttcggctt cttcactgcg tcgcagtgac gatgagaatc
     8641 gcgagctgac cacgatacga tgtgcaaaca cgggcacgac gagtcgcttg atatcgtcgg
     8701 gaatgcagta ggtgcgatct tcggtgagcg cgagagcctg ggcggcgcgg aagagcgaga
     8761 gcgttccgcg cggactgacg ccgagatcca gcatctccga actacgcgtc gccgcaacga
     8821 tccgcatcag gtactgaacg agtacgtcgt caacggcgac actcgcgaca gtctgttgca
     8881 actctacaac ttcacgcgcg ctcatcaccg gttcgagctc gtttaatgga tccctgaact
     8941 cacgatcgcg caggatcttt tgttcgtcag ccggcgccgg atagccgatg cgcaatcgca
     9001 gcatgaagcg atccagttgc gactcgggca gcgggtacgt tccgtgatgc tcgatgggat
     9061 tctgtgtcgc gagcacgatg aaaggcgcgg gcaaatcgtg cgtcatacct tccacggtca
     9121 cgtgtcgctc agccatggct tccaataatg ccgactgggt tttgggcgtg gtgcgattga
     9181 tctcgtcggc cagtacgacg ttggcaaaaa ccggccccgg tttccaatca aagaggccgc
     9241 tgtgctggtt gaagacggaa agaccgatca cgtctgacgg cagcaggtcg gaagtaaatt
     9301 ggatacgctg aaaactgcaa tcgagcgctc gggcgagtgc ctgagccagc gtcgttttgc
     9361 ccacccccgg aacgtcctcg accagcaggt ggccaccggc aatcagcgta acaatggcca
     9421 gtcgaatcgc ctcaggctta cccttgatga cggtttcgac agcctgttgg agccgttcaa
     9481 tctgctcgac cgcgttggcg ctggctgtct gttcgctgct gatggactcg aggttccgca
     9541 tgaattggcc taattctaca tggtaagagc acgcgtcgca cggaaaagtt ggctattgcg
     9601 acaatgtgac cttcacacgc tcctgtagta gactaagcgc ctgacagagg acgggcaagc
     9661 gtaagcagaa ttcggaacgg aaatttaaga gcttcgtgta agtgcccgag gagaacttat
     9721 ggaacccctg aataatagca aaaagaacca gcgcggcttt tcgctaatcg aattgatgat
     9781 cgttatcgcc attatcggca tcctcatcgg agtcggaatc gcgggataca aggcagccat
     9841 cagagcgaca aacgaagcgg cggcggtgaa gacgctacgc tcgatcgccg agcaacagat
     9901 gctttatttc aactctcacc agcgcaactc gtttggaacg tttgaggaaa tgcgcaaaga
     9961 gaacctgctc gatagccggt tcgacggcag cacgccggtg gtcgatggct acgtctacac
    10021 gatgaaggtg atcccgaagt cgacttccgc acagcccggc tacacgataa acgccgatcc
    10081 acaggtgccg acgggagttt cagcgacagg gaagaacttc tattacgtcg actctgatac
    10141 aaacacgatt cacgtcaacg cgaaccagca ggcgaccgcg accgatcctc cgatcggaca
    10201 gtaagtatta gccacaaaag gcacaaaaag cacaaaagag atttttttgt gctttttgtg
    10261 cctttttgtg gctaaaaagt cttcttccct tccaggcgct caatcctttg atcaggtgcg
    10321 gcttcacgtt gtgttttgac gtgaacgaac tcgacatccg tcacatcatt cagccagaaa
    10381 gccggccgta gatccggact caaataactc acttcaacat cacgcatctg caaacccttc
    10441 acgtgcctga tgaagaaccc gtacgccggc aactcaccaa acatcgtcgg ctcaggataa
    10501 agagcctctc gctccggcgg ttcgagcgcg gcctgctcct ttgtgccacc gcctttcgcg
    10561 taaatgcgga tgttgttgag ctgcacatct tcgatcagga accccggtat gccgctgatg
    10621 atcgacgagt acttcggatc ggcgttgtaa acgacgacat tactgatgat cacgcgacgc
    10681 agcgcgccgg ttttggttgt ctccttcggt ccgcgattgc gcgcgccgag tcgcaggaag
    10741 atcggagagt tgctgatgtc gcgcatcgtg atgtttgaga tcgtcacgtc ttcgagcagc
    10801 gcgccgtcaa ccgcttcgag cgcgagtcca cgcgagtaat caaagacaca gttcgagacg
    10861 gtgatgtttt tgaagccgcc gttcgactcg gtgccaaact taattcgtcc cgtcgggctg
    10921 aacgtgccgt ttgcgttgcg gaaatcacgc ttataagtgc catcgagaag tgtgccttcg
    10981 tcgtaaccgc tgacctggca attcgtgatc gtcacgtttt ccgtgacgcg cgcatagccg
    11041 agcgcgtacg agctcttcgg gcagatccca tcatcgaacg gtgagttgac ggtgcagttt
    11101 gagacacgaa cattcttgca cgcatcgacg tcgatgccat cgcgattcgt atcgatcttc
    11161 acgttgtcga tggtgaggtt gtcgacgccc gtcgcgagta tggcgaagtg tccgccgtgc
    11221 aggatgctga tatcgcgaat gatgacgttg cgagagagtt tcagactgat cgctttgttt
    11281 ccccaacctg gctcgactgc gtcgcgcgca tttggataac cgaacgggcc tgctttcgga
    11341 tcgggtccct gcttctcgag tgcatcgttc tgctctttcg tgcgcgactg gctgccactg
    11401 cgcaccagtc ctttccccca gatacgaccg ggaccgagga tcgagatgtt ttccaggttc
    11461 tcgccccaga tcagactgtt atgccagtgg ctgtgaccga agtcctgata ctggtcccac
    11521 tgattcggct cgggcagatc gtatttgccg tcgccatcac gcggatcggc agcgaggatc
    11581 gtcgcgccct gatcgagata aagcgccacg ttgctcttca atcgaatcga gaaggcgcga
    11641 tacgtgccgg cagtaaatag gaccgtgcca ccgcccgcag ccgccgcggc atcgatcgct
    11701 ttgttgatgg cagggctgtc gagagctttt ccgtcgcctt ttgcgccata ctttctgacg
    11761 tcgaaggcgc tggctttttg cgcaacggcg attgtggcgg tcgagtgaac tgtatgaccg
    11821 gaagtgaatg cgctggaagc gagcgctaga atcagcaggg agagcgtaaa tggtctaatc
    11881 atggagcggg agtttctcac gaattgacgg atgcgtgaaa ctttactgag ttttttagac
    11941 gactgccgcg cgcacggcga gcagacagca gttacacacg aggcgggagc gcgtccttca
    12001 cgctggtcct acgcgcatat tgcttcgtct gcgtctcagt ttgcgcgcga gcttgagacc
    12061 cgcggtgtca gtaccggcga ccgcgtgctc ttttggggcg agagtcgtcc ggaatggatc
    12121 gcgtgctttt acggctgttt gctgcgcggc gcggtcgtgg ttcctttaga tctaaaatca
    12181 gtgccggatt ttgtcgtgcg tgtgcagcaa caagtttccg caaaactcct cctcgcagac
    12241 gagccgcaac ttgatattcc tcatctcaca ttcaacagtc tcaccgatct acttgcacga
    12301 cacccagacc aaccatattc gacaaaggcg aaacccgacg acctggtcca aatcgtcttc
    12361 acttccggca cgacggccga accgaaaggc gtctgtctca cacaccgaaa tctgctcgcg
    12421 aacatcgcgc ccatcgaaaa agaattcaac cgctacagac gctgggaaaa gttcgtacac
    12481 ccgttacgtt ttctctgtct gctgccgctg agtcacgtgt ttggacagat gatgggcatc
    12541 ttcataccgc agctactcgg cgccgaagtc tacttcagcg agtcatacaa accctcgcaa
    12601 atcatcaggt cagtgaaaaa gcatcgcatc aacgccgtcg tcaccgtgcc gcgtgtgctc
    12661 gaaacgctgc gcgagaaagt gaaagctgaa ctcacggacg atcagatccg cgcggcccga
    12721 ggacgtcact ttctaagaaa ctggtgggca ttcagaaaga tccaccgcca gtttggctgg
    12781 cgcttctggg cattcatcac cggcggcgcg actctcgaaa gcgaaacgga aactttctgg
    12841 cgtcgactcg gctacgccgt cattcaagga tacggcatga ccgagactgc ttcgctcaca
    12901 agtctttcgc atccgtttcg catgcgacac ggttcgatcg gaaagccggt cgccgggcag
    12961 gaagtgaaac tgagcgacga cggcgagatc ctcgttcgcg gtgaaaacgt ttcgcacggt
    13021 tactggaatg gtaacggtca gtcgcttaca aacgacgaag gctggattca cacgggcgac
    13081 atcggcgagt ttggtccagg tggcaacata tatttccgtg ggcggagtaa agaaacgatc
    13141 gtcactgcgg cgggcctgaa gatctacccg acggatctcg aagctgcact cgatcgtcaa
    13201 ccggagataa aagcgagcgc cgtgattcca ctcaccggag gcagcgaggc gctggctgtg
    13261 ttgattcctc acgaagaagc agatctgagc gccgcggtgc aacgcgccaa cgaatcactc
    13321 gcggagtttc aacggattcg tcactggatt gcctggccga atagcgactt tccgcgcaca
    13381 ccgggcacgc gcaaagtaat caaaggacag attgcacaag ctgtgcagac gatgctgcaa
    13441 ccgtccacat ccgcagccgc tgataaagat ctggctccgt taagtcttcc ggtcatcagt
    13501 cgtataacac gcgtcgagcc cgccgcgctg agtccgaatg caaaccttgc cgacgatcta
    13561 aaactcgatt cacttggcag ggttgagttg ctaagcgcgc tcgaagacca gtatcagatt
    13621 gagctggacg aagccgcgat cactgaagca acaacgatcg ccgacatcga acgcatcgtc
    13681 agtcgtggta aatccgaagc cgtcgcttat ccatacccgc gctgggccat gcgtttcccg
    13741 ttcacgtgga tccgcttcgt ggtctatcac gtgtttttcc tgccgctgac actgatcatg
    13801 tgtcgtgtgc gaacgatcgg tgtcgaacga ttcgcgaagg tgaagccgcc ggtgctgttc
    13861 atttcaaatc acgtgactga cgttgatgcc gcactgattc tctccgcctt gccgtggcgg
    13921 tggcgttatc gaatggcgat tgcgatggcg ggcgaaatcc tgcgcgagtg gcgaatgaac
    13981 gcgaaagtcc aatacgcgct cggcgcagcg ctattcaatg tgttttcgtt gccgcggcag
    14041 agtgggttca ggcagagctt tgagtacgca ggtgaagcgg tcgatcgcgg ctttagtatt
    14101 ttgattttcc cggaaggaac agaaacgaag gatggtcaac tccagccctt caaggccggc
    14161 atcggattaa taacgtcaga actaaaggtg cccgtcgtgc cggtaatgct gcgcggcctc
    14221 ttcgaggtta aaaaacgtaa acagcgcttc gtaaaacccg gcacggtatc gattactttc
    14281 ggcgacccgg tagaattctc ttccgaccaa acaccctccg acataacacg agatctcgaa
    14341 ctcaggctac aatccgttca ataatccgcg cagatccgcg ttacgctacc tggtaaacct
    14401 gaatcgcctg ctctcttccg cgaacttcga cctcacccat cggcaccact ttacttcggt
    14461 cactacttac cgcctgccac accatctccg aaatcaacaa ctgtgaatca aatctcttat
    14521 tcaacttctc aatccgcgcc gcgagattca ccacgtcccc aatcactgtg tactcccgtc
    14581 tcaacgccga tccaatactc cccgtcacag cttccccggc atgtagccca atgcccacgg
    14641 tcgtcggcag gatcgtcccg gcctcaacct cgtcacgtac gcgcgcgaga atctcctgcg
    14701 cggcgtccag cgcgttcgcg caatctctcc catctgaaag cggcgcgcca aacac
//
LOCUS       DASSFQ010000138        12085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2139748, whole genome shotgun sequence.
ACCESSION   DASSFQ010000138 DASSFQ010000000
VERSION     DASSFQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12085
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139748"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFH96_04790"
     CDS             <1..720
                     /locus_tag="VFH96_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET6973316.1"
                     /translation="QLGGNISFNRFQTTYRHYYKASWLRETVLAGNMTLGLANVFNPR
                     DRDGNGAIDEIDLTLPISERFFSGGSTTLRGFSFEEAGPRQVVIPEGEFRDSDKNVVF
                     LNPFTVPVGGNALAVLNLEARVPLTRAVQVVPFYDGGNVFRRIGDLFGKEDTTPVPPG
                     DILAAINKANLRAHWTNTVGLGFRLQTPFGGALAVDYGFLLNPPEFLIPQRGPTSIFD
                     GTPAVFRLHRGNLHFRITQTF"
     gene            730..1404
                     /locus_tag="VFH96_04795"
     CDS             730..1404
                     /locus_tag="VFH96_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973317.1"
                     /translation="MTRILRPIFTALVVGFVCALAPVCSAQQVVDKMVATVNAGVRTD
                     LITYSDLMWQLALQPRTVLDNPSSQDLNRALRLLIDQRLILQEAEKIPTIVPTQKEVS
                     DARDALARNFTSVTEFQERLQRVGLTSEKLNEIVEQRLKMEKYLDFRFRNFVVISQKE
                     IADYYRDVYTPRVHARLPGRIVPPLEQVRDEIEKTLMEAKIESDTDAFLDQARERAEI
                     VTLNPV"
     gene            1410..2228
                     /locus_tag="VFH96_04800"
     CDS             1410..2228
                     /locus_tag="VFH96_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6973318.1"
                     /translation="MKIGLALSGGAARGMAHVGVLRALLEHGIRIDCIAGTSAGSIVA
                     GAFAAGMSIDEIADLGRKLRWRHVGRITLSRLGLQTNARLEKFMRDRLPITKFEDLPI
                     PFAAVATDLKTGEPVVMKDEGDVPFAIRASCTIPGWYVPVPDREGRQLVDGGLVAVIP
                     SAATRALGADLVISVDVNSAGATFLPTFLSSSGSLFGVLLQSMMLIQRSVCKQHCELS
                     DLVITPKIGHIRWDQVRRADELMTAGYEAGLESIPKVRALIDSRPEVPQLLSIS"
     gene            2257..4662
                     /locus_tag="VFH96_04805"
     CDS             2257..4662
                     /locus_tag="VFH96_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET6973319.1"
                     /translation="MVIEAKSLPDPRRSRVKRRFVRLGLVLILIAGAVAADAVARSYH
                     YYSQIIDARLASGYLTSRPGLYAAPRVLAAGQKLSREKLVAVLRRAGYVEGNASDVWS
                     GSFSLVDTDLEIRPARAKQVVRVTFAGDEITEIRGDGALLDSFTLEPEILSTDLSSKA
                     GKRELLSFGEIPAALVHAILAIEDRRFFEHSGVDINGLARALLRNVTDEQMAQGGSTI
                     TQQLIKNTYLTPEKTVQRKYAEAMLSFALERRLSKNDIFALYCNEIYMGQRGAVAVRG
                     VKEAAKVFYGKELRDLTLSEAATLAGMIQSPARYSPLQHPEAARARRDIVLAAMRENG
                     WITAAQNTSANAEAVTVAPAPVVDDSLAPYFVDYVNRLAGSQFETSADRQRIYTTIDL
                     ELQQAAERALQKQLDRLDVVYASRGLKPQAALVALDPHTGNVLAMVGGRKYAESQLNR
                     ATDALRQPGSTFKPFVYAAAVEDGFSPVRMFMDAPREFTYDRDKIYRPANYGGGYSMT
                     EVTMRTGLVRSLNVVTVDIALQTGLARISNLAVRFGLPKPERYPALALGTEEVTPLQL
                     ASAYATFVNEGQRVEAKAVTSVGEPPATHGAPVAVDQVVSPATAYMITNMLQGVVERG
                     TARKARGAVPGTAIAGKTGTSRDGWFVGYSPNLVCVVWIGFDDNKQLGLTGAEAALPA
                     WVDFMNDAVAMRPDLGGENFECPAGIEFVEIDSATGLRSTVTCPVRELIAVTPRMAPT
                     MECYLHGNLPAQQGGGSPFAEEVETKHEEIAVKKEMPLPNHRATRVDVDLTGRRSLVN
                     DMR"
     gene            complement(4732..5109)
                     /gene="acpS"
                     /locus_tag="VFH96_04810"
     CDS             complement(4732..5109)
                     /gene="acpS"
                     /locus_tag="VFH96_04810"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET6973320.1"
                     /translation="MIISIGIDIIEVARIREVLLRTPRFADRVYTAAERAYCDSRGVV
                     AAQHYAVRFAAKEAALKALQTGWRGGISWQDVEISAHESGAPYLIFRGEVLAVFEKFR
                     ATATHLSMSHTSEHAIAQVILER"
     gene            5102..5953
                     /gene="pgeF"
                     /locus_tag="VFH96_04815"
     CDS             5102..5953
                     /gene="pgeF"
                     /locus_tag="VFH96_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET6973321.1"
                     /translation="MITSDNQLTEAGFYWRETEGVRALVCAPLEADGFVNGFSTRLGG
                     VSDMPANALSLAGFHDDAAENILENRRRFLKLFPGAWTLAGCWQVHGADVRVVHTIEE
                     AKPAENSLGETVYCDVIVSDAKGVLAGVKTADCVPILIGDPVTRAFAAVHAGWRGTLA
                     TAVIAGVERLAKEYDARPEDLRVAIGASAGPCCYEVGSEVIEAFSSKFTDGEKLFTPT
                     RAGHATVDLLKANRDQLISTGVKPERIHIAPICTMCRTDLFFSYRKEKSVHGKVGRLM
                     AVVGRKS"
     gene            complement(6025..6516)
                     /locus_tag="VFH96_04820"
     CDS             complement(6025..6516)
                     /locus_tag="VFH96_04820"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HET6973322.1"
                     /translation="MNKLRHVLPLFIILLLPVTFYSNPAPQSSSDVNVSGSLAPDKIK
                     KGRVVRASVVMDIPNGLHVQSSKPLDKFLVATKLDVETPSGLQVGPISYPRALMRNLK
                     FSKGAVAVYEGKAIMRFNVTVPANYSGGSGEIKGKLRFQACNDESCFPPVTRAVKMWL
                     NVE"
     gene            complement(6516..7607)
                     /gene="rlmN"
                     /locus_tag="VFH96_04825"
     CDS             complement(6516..7607)
                     /gene="rlmN"
                     /locus_tag="VFH96_04825"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET6973323.1"
                     /translation="MEKAVQLTGLTQPEMVSFVETLGEPAYRARQLFGALQHRRLRSF
                     DDITDLPKELRTRLNEIARAATLKVESQYLSDDGTRRYLMKTDDSLPVETVFIPEERR
                     DTICFSSQSGCPLQCTFCLTAQLGLLRSLSAGEIVEQIQIALNDAYGTGVKPPRGTNL
                     VAMGAGEPLLNLDQLLQALRVMADPQGMHIVPNRVTVSTAGVVPRILDLANTSDRPHL
                     AISLAAPTDDLRNELMPINKRWPLDELLGACKVFEKTLKPNERLTFEYVMLEGVNDSD
                     KQARQLANLLNRHTIKSKVNLIPHNPADPLPYQPSSDERVEQFKNILESKGIHTYVRR
                     PRGRDIFAACGQLAARQTTLTNSNLVGAN"
     gene            7687..8553
                     /locus_tag="VFH96_04830"
     CDS             7687..8553
                     /locus_tag="VFH96_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6973324.1"
                     /translation="MFASRGFLTRERLIGVVVALVLGCVAFVYGLRQLELFITFHPAR
                     MTALESKSPAEGAEIVWFNSADGTRLNGWFFESQSKPAVATVIFCHGNGGNISSVGWM
                     GQRFAKHGFNVLLFDYRGYGASDGVVADESGLYADGDAAVAYVVNNKGVRPEQIILYG
                     HSLGTTVVADVASRGNYGAVVLESGLSSASSVAATALPWLPAWLHFLGRNRFESARKL
                     ANVKSPILIAHGDPDGTIPTDEARRLFASANEPKKLLIVPGAGHVVFAAAGEPYLNQV
                     EQFMRDAMIRGR"
     gene            complement(8540..10138)
                     /locus_tag="VFH96_04835"
     CDS             complement(8540..10138)
                     /locus_tag="VFH96_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HET6973325.1"
                     /translation="MLRRFVTALLLALISIASISAQQTKRNPEEQPRKVKQEPKKAYV
                     NWINDVDLILTQSEREAWKKLATDDEREKFIEDFWRSRDPDPDTEENEFKQEFFERIA
                     YANEHFSSGKPGRLTDRGRIYIKFGKPDEVESHPAGGSYDRPSYEGGGSTSTYPFEKW
                     FYRNIPNVRSGVELEFVDPTGSGEYRLARNPDEKDALINIPGAGPTFAELAGLETRAD
                     RIANLGGFGRVNYMRSQDSPFEVLDLIRSLDRDQPFERNFLGTKTPTPIIDDNPLNFE
                     TQLNYFKQSDNRVLAVLTVQANNDQLSFANSGGIETARLNIFGRVITLTERQIGKFED
                     SVTTTATSQELSTTRTRKSAYARAFILDPGRYRVDVIVRDIQSGAAGMKQLGFVVPEF
                     PTDRLSASSVVLAAKLENMEGGAAVGPFVIGTTKVIPNLSGVFRRNQPVGVYLQIYNA
                     AIDQTTLRPAADAEYVLLKNGKELSKQTEDWRQINDAGQRLTLSRLIDSRSLEPGEYQ
                     IQIRIHDHVSGETITPTAKFTIVP"
     gene            complement(10239..11852)
                     /locus_tag="VFH96_04840"
     CDS             complement(10239..11852)
                     /locus_tag="VFH96_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HET6973326.1"
                     /translation="MSYQKFVLRLALIVSLFPSASPRLFAQDQQDPSNKPRNVKPELK
                     KAYKDWLDKDVTYIITDEERKAFKKLATDDERERFIEEFWRRRDPDPDTDENEFKEEY
                     YERIAYANEHFSSGIPGWKTDRGRIWIMYGKPDERETHPMGGSYERPSYEGGGSTTTY
                     PFEIWFYRYLAGVGSGVEIEFVDPTGSGEYRIARNPNEKDAMLMVPGAGLTLSEQLGL
                     TSKADRVAGIGGVGNQNYQREQDSPFSRLQLLADLNRPPQVKFNDLATAVNTGVVEEN
                     PLNFDLRIDFFRQSDERVITAFTIQAENKDLVFQDSGGLQQARMNIFARITSVAGRRV
                     GIFEDPVITTATATELSEAKDRKSAYQKAVPLAPGTYKVDVIVRDVASGATGLKQLGF
                     TVPKYDQEKLSTSTLILAAKLESLVDQPAVGQFVIGTTKVIPNVSGIYKRGQPVGVYL
                     QVYNAGIDQTTLRPSVDVEYSLMRDGKELGKQAEDWRGMSDSGQRLTLARLIDTRNLG
                     PGDYEVAIRIRDRVTGQSLSPSQKFTVTQ"
BASE COUNT         2468 a         3036 c         3649 g         2932 t
ORIGIN      
        1 caactcggcg gaaatatctc gttcaatcgt tttcagacca cctacagaca ttactacaaa
       61 gcgagttggc tgcgcgaaac ggtgctggcc ggcaacatga cgctggggct tgcgaacgtg
      121 ttcaatccgc gcgaccgcga cgggaatggc gccatcgatg agatcgattt gacgttgccg
      181 atcagcgaac gatttttctc gggcggctcg actacgctgc gtggtttcag tttcgaagag
      241 gctggtccaa gacaggtggt cattcctgaa ggtgagtttc gagattcgga taagaatgtg
      301 gtgttcctga atccgtttac ggtgccggtc ggtggaaatg cgctggctgt tttgaatctc
      361 gaagcgcgag tgccgctgac gcgcgcggtg caggtggtgc cgttttatga tggcggcaac
      421 gtgtttcgtc gcatcggcga tttgtttggg aaggaagata cgacgcccgt tccgcccggc
      481 gacattttag cggcgattaa caaggcgaac ttgcgagcgc attggaccaa cacggtgggg
      541 ctgggattcc gtttgcagac gccgtttggc ggcgcgctcg cggtggatta tgggtttctg
      601 ttgaacccgc cggagttttt gataccgcag cgtggtccga cgagtatttt tgacggcaca
      661 ccggcggtat ttcgactgca ccgcgggaat ctgcatttca ggattacgca gacgttctga
      721 ggaacacgga tgacacggat attaagaccg attttcactg cgttggttgt ggggttcgtt
      781 tgcgctctgg cgccggtgtg ctcagcgcag caagttgtgg acaagatggt ggcgactgtc
      841 aacgcgggag ttcgcactga tctgatcaca tattcggatc tcatgtggca gttagcgctg
      901 cagccgcgta cagtgcttga taacccgagt tcgcaggatc tcaaccgcgc gttgcgacta
      961 ttgatcgacc agcgcctgat tcttcaggaa gcagaaaaga tcccgacgat cgttcccacg
     1021 cagaaagaag taagtgacgc acgtgacgcg ttggcccgca actttacctc agtaacggag
     1081 tttcaggagc gtctgcagcg agttggatta accagtgaga aactcaatga gatcgtcgag
     1141 cagcgcctga agatggaaaa atatctggat ttcaggttta gaaatttcgt agtaataagc
     1201 cagaaggaga ttgctgatta ctaccgcgac gtttatacgc cacgcgtcca cgcccgttta
     1261 ccgggacgta ttgtgccgcc gctggagcag gttcgcgacg agattgaaaa gacgttgatg
     1321 gaagcgaaga ttgagtctga cacggatgca tttctcgatc aggcgagaga acgggcggag
     1381 attgtgacac tgaacccggt ttagtcttca tgaagattgg attagcactt tccggtggtg
     1441 cggcgcgtgg catggcccac gtcggcgttt tgcgagcgct gctggagcac gggattcgga
     1501 tcgactgtat cgccggaacg tcggcgggat cgattgtcgc cggcgcattc gcggccggga
     1561 tgtccatcga tgaaatcgca gatctcggcc gcaagcttcg ctggcgtcac gtcggtcgta
     1621 tcaccttgtc gcgactcggc ctgcaaacca atgcgcgtct tgaaaagttt atgagagacc
     1681 ggctgccgat cacgaagttt gaagatctgc cgataccgtt tgcggcggtg gccactgact
     1741 tgaagacagg cgaaccggtg gtgatgaagg atgaaggcga cgtgccgttt gcgatccggg
     1801 cgagctgcac gattcccgga tggtatgtgc ccgtgccgga tcgggaaggc cggcaactcg
     1861 tcgatggcgg gttagttgcg gtgattccgt cggctgcgac acgcgcgctc ggcgcggacc
     1921 tcgtgattag cgttgacgtg aatagcgcgg gtgcgacttt tctcccgact tttttatcgt
     1981 catcgggttc tctgttcggg gtgttgttgc agtcgatgat gctgattcag aggtctgtct
     2041 gtaaacagca ttgcgagctc tccgatctcg taattactcc gaagattggt cacattcgct
     2101 gggaccaggt gcgccgcgcg gacgagttga tgaccgctgg ttacgaagcc gggctcgaaa
     2161 gcatccctaa agttcgagcg ctgattgatt cccgtcctga agttcctcag ctattaagca
     2221 tttcttaaaa tcacaggata tcggaagcac tgaaggatgg ttatcgaggc taaatctctg
     2281 cccgatccac gacggagccg tgtcaaacga cgctttgttc gactcggcct cgtcctgatc
     2341 ttgattgcag gcgcggttgc ggctgacgcc gtggcgcgtt cttaccacta ttactcacaa
     2401 atcatcgatg ctcgactcgc gagtggttat ctgaccagcc ggccgggact ctacgccgct
     2461 ccgcgcgtgc tcgcggccgg gcagaagtta tcgcgcgaaa agctagtcgc ggtgttgcgt
     2521 cgtgctggtt atgtcgaggg aaacgcgagc gacgtttgga gtggaagttt tagtcttgtc
     2581 gatacagatc ttgagatccg gcctgctcga gcgaaacaag ttgtgcgagt gacgtttgct
     2641 ggtgatgaga ttactgagat tcgtggtgat ggtgctttgc tcgactcatt tacactcgag
     2701 ccggagatct tatcgaccga tctttcttca aaagccggca aacgcgaatt attgagcttt
     2761 ggcgagattc cggcggcgct ggtccatgcg atactcgcca tcgaagaccg gcgcttcttc
     2821 gagcattcgg gtgtagatat aaacggtcta gcacgcgcac tgttgagaaa tgtcaccgac
     2881 gagcaaatgg cccagggcgg atccacgatc actcagcagc tcattaagaa tacttatctc
     2941 acgcccgaaa aaactgttca gcgcaagtat gcggaggcaa tgctgtcgtt tgcactcgaa
     3001 cgccggctct cgaagaacga tatttttgcg ctctactgca acgagatcta catgggccaa
     3061 cgtggcgctg tggccgtgcg tggcgtgaag gaagcggcaa aggtttttta cggaaaagag
     3121 cttcgcgatc tcacgctgtc ggaggcagca acgcttgccg ggatgattca gagtccggcg
     3181 cgttactcac ctctgcaaca tccggaagca gcacgagcgc gaagagatat cgtcttggct
     3241 gccatgcgag aaaatggctg gatcacggcg gcgcaaaata cgtcggcgaa tgcggaagcg
     3301 gttacggtcg cgccggcgcc agtcgtcgat gattcacttg cgccttactt tgtcgattat
     3361 gtgaatcgtc tcgctgggtc gcagtttgaa acgtcagcag accggcagcg aatctacacc
     3421 actatcgatc tcgagttgca gcaagcggct gagcgagcgt tgcagaagca gttggatcgt
     3481 ctcgatgttg tttatgcaag tcgtggattg aaaccgcagg ctgcgcttgt cgcgctcgat
     3541 ccgcatacgg gcaacgtgct cgcgatggtt ggtggccgga aatatgcgga gtcgcaactc
     3601 aatcgtgcta cagacgcgct gcgtcaaccg ggatcgacgt tcaagccgtt tgtgtatgcg
     3661 gctgcggttg aagacgggtt ctcgccggtg cggatgttta tggatgcgcc gcgagagttt
     3721 acttacgacc gcgacaaaat ttatcgaccg gcaaattacg gcggcggtta ttccatgacg
     3781 gaagtgacga tgcgcacggg gctcgtgcga tcgttgaatg tggtgacggt tgacattgcg
     3841 ttgcagaccg gactcgcgcg catttcgaac ctcgcggtgc gatttggttt gccgaagcct
     3901 gagcgctatc ccgcgctcgc gttggggacg gaggaagtga cgccgctgca gttggcctcc
     3961 gcatatgcca cgtttgtgaa tgaaggccag cgtgtcgagg cgaaagctgt aacgagtgtg
     4021 ggcgagccgc cggcgacgca tggggctccg gttgcagtgg accaggttgt tagtccggcg
     4081 acggcttaca tgattacgaa catgcttcag ggcgtggttg agcgtggtac ggctcgcaaa
     4141 gcacgtggag cggttccagg tactgcgatc gccgggaaga cgggcacgtc gcgcgatggt
     4201 tggtttgttg ggtattcacc gaacctcgtg tgtgttgtgt ggatcggttt tgatgataac
     4261 aagcagcttg ggttgaccgg ggctgaagca gcgctgccgg cgtgggttga ttttatgaat
     4321 gatgcagtgg cgatgcggcc cgatcttggt ggtgagaatt ttgagtgtcc ggcggggatt
     4381 gaatttgtgg agattgattc cgcgaccgga ctgcgttcta ctgtcacgtg tccggtgcgc
     4441 gagttgattg ctgtcactcc gcggatggcg ccgaccatgg agtgttatct acatggaaat
     4501 ctgccggccc agcagggcgg cggcagtcca ttcgctgagg aagtcgaaac aaagcacgaa
     4561 gagattgcag tgaagaaaga gatgcctcta ccaaatcaca gggccacacg ggttgatgtt
     4621 gatttgaccg gacggcgatc gcttgtcaac gacatgcgtt agacaggatt tacaagattt
     4681 ttcaagattt atctcgtgaa tcctgcaaaa tcttgtaaat cctgtctgca actatctttc
     4741 caatatcacc tgcgcgatgg cgtgctcgct cgtgtgtgac atcgataagt gcgtcgccgt
     4801 cgcacgaaac ttttcaaaca ctgccagcac ttctcccctg aagatcaagt acggcgcacc
     4861 gctctcatgc gctgatatct ccacgtcctg ccagctaatt ccgccacgcc atccggtttg
     4921 cagtgctttc aatgccgctt cttttgccgc gaagcgcacg gcataatgct gcgcggctac
     4981 gacgccgcgc gagtcgcagt atgcgcgctc ggctgcagtg tagacgcggt cagcaaagcg
     5041 cggcgtccgt aggagcactt cgcgaatgcg cgcgacttcg ataatgtcta taccaatgga
     5101 aatgatcaca agcgacaacc aactaaccga agctggattt tactggcgcg agaccgaagg
     5161 cgtccgcgcc cttgtttgtg caccactcga agcagatggc ttcgtgaatg gcttctcaac
     5221 gcgtctcggc ggcgttagcg acatgcctgc gaatgctttg agccttgccg gctttcacga
     5281 cgacgcagcg gagaacattc tggagaatcg tcgccgcttt ctcaagcttt ttccgggcgc
     5341 gtggacgctt gctggttgct ggcaggtcca cggcgccgac gtgcgcgtgg tccacactat
     5401 agaagaagcg aagccggctg agaactcgtt aggtgagact gtgtactgcg atgtgatcgt
     5461 cagcgacgcg aagggcgtcc tggctggagt taagactgct gactgcgttc cgatcttgat
     5521 tggcgatccg gtgacgcgtg cgtttgcggc agtgcatgct ggttggcgtg gcacattggc
     5581 gaccgcggtg atcgcaggtg tcgagcgatt ggcgaaggag tacgatgcga ggcctgaaga
     5641 tttgcgtgtg gcgattggtg cgtctgctgg gccgtgttgt tatgaagtcg ggagcgaggt
     5701 gatcgaggcc ttttcgagta agtttaccga tggagaaaag ctgtttacac cgacgcgagc
     5761 gggtcacgcc acggttgatt tactgaaagc aaatcgcgat cagttgatat cgactggagt
     5821 gaagcccgag agaattcata tcgcgccgat ctgcacgatg tgccgtacgg atcttttttt
     5881 ctcttatcga aaagagaaga gcgttcacgg aaaagtgggc cggttgatgg cggtggttgg
     5941 aagaaagtcg tagacaggat ttacatgatt tagacaagat ttacatatta atcctgaaaa
     6001 atcttgtaaa tcttgtctaa cgacttactc gacattgagc cacatcttca ctgcgcgcgt
     6061 aacaggcggg aagcacgact catcattgca cgcctggaaa cgaagcttgc ccttgatctc
     6121 acccgatccg ccggaatagt ttgcgggcac ggtgacgttg aaacgcataa tcgccttgcc
     6181 ttcgtaaacc gcaaccgcgc ccttcgagaa tttcaaatta cgcatcaacg cgcgtggata
     6241 cgagatcggg ccaacctgca gtcccgacgg tgtctcgacg tcgagctttg tagcgacgag
     6301 aaacttatcg agcggcttgc ttgattgcac gtgcagcccg ttcggaatat ccatcacgac
     6361 gctcgcgcgc acgacgcggc ccttcttgat tttgtctgga gctagcgagc cgctaacgtt
     6421 cacatcactc gacgattgcg gcgctggatt cgaatagaaa gtaacgggca gcagcagaat
     6481 gatgaataac ggcaagacgt gacgaagttt gttcattagt tagctccgac tagattggaa
     6541 ttagtaagtg tagtttggcg tgccgccagt tgcccgcagg ccgcaaaaat atctctgccg
     6601 cgcgggcgcc gcacatacgt gtgaatccct ttcgattcga gaatgttttt gaattgctcg
     6661 actcgttcat cgcttgacgg ttggtacggc agcgggtccg caggattgtg cggtatcaag
     6721 tttacctttg acttgatcgt gtggcggttg agcaggttgg cgagttgtcg cgcctgcttg
     6781 tctgaatcgt taacgccttc gagcatcacg tattcaaacg tgagtcgttc gttgggcttc
     6841 agcgtttttt caaatacctt gcaggcgccc aaaagctcat cgagcggcca acgcttgttg
     6901 atcggcatta gctcgttgcg caggtcatca gtcggcgccg cgagtgagat cgcgagatgc
     6961 ggtcgatctg aagtgttcgc caggtcgagg atgcgtggga cgacaccggc agtcgaaact
     7021 gtcacgcgat tcggaacgat atgcatgccc tgcggatccg ccatgacgcg caaagcctgc
     7081 aggagttggt ccagattcag cagcggctcg cctgcgccca tcgcgacgag attggttccg
     7141 cgtggcggtt tgacgccggt tccgtaagcg tcgttgagtg cgatctggat ttgttcgacg
     7201 atctcgccgg cgcttaacga acgtagcaaa ccgagttgtg cagtgagaca gaaagtacat
     7261 tgtaacgggc agcccgattg cgacgagaaa cagatcgtgt cgcggcgttc ttcgggaatg
     7321 aagactgttt cgacgggcag actgtcgtcg gttttcatta agtagcgacg cgtgccgtcg
     7381 tcagaaaggt attgcgattc gaccttcagt gttgcggcgc gcgcgatctc gtttagtcgc
     7441 gtgcgcaatt ctttcggtaa atccgtgata tcgtcgaaac tgcgcagacg gcggtgttgc
     7501 agcgccccaa agagctgccg cgcgcgatag gcgggctcgc ccagcgtttc gacgaatgag
     7561 accatttccg gctgagtaag cccggttaac tgcactgctt tttccatgca gggtcatctt
     7621 agcatttgag acggcgcgtc tcatccgcgc ggaacctgga tagtcccggc acacgtagta
     7681 atcgacatgt ttgcctctcg cggctttctg acacgtgaac gtttgatcgg ggttgtagtg
     7741 gcgctcgtct tgggttgcgt ggctttcgtg tacggattgc gtcagctcga gttgttcata
     7801 acgtttcatc ccgcccgcat gactgccctc gagagcaaat cgcctgccga aggagcggag
     7861 atcgtttggt tcaattccgc tgacggaacg cgcttgaacg gatggttttt tgaaagtcag
     7921 tcgaaacctg cagtcgcgac cgtcatcttc tgtcatggca acggcggaaa tatctccagt
     7981 gttggctgga tgggccagcg gtttgcgaaa cacgggttca acgttttgct tttcgattat
     8041 cgtggctatg gcgcgagtga cggagttgtt gctgatgagt cagggcttta tgcggatggt
     8101 gatgccgccg ttgcgtatgt ggtgaacaac aaaggagtgc gtccggagca gatcattctg
     8161 tatggtcatt cgcttgggac gactgtcgtt gcggatgttg cttcacgcgg gaactatggc
     8221 gcggtggtgc tcgagtcggg actcagctca gccagttcgg ttgcagcaac tgcattgccg
     8281 tggttgcctg cgtggctgca ctttctaggg cggaatcgtt ttgaatcagc tcgaaaactc
     8341 gcgaatgtta aatcaccgat tctgatcgcg cacggcgatc cggacgggac cattcctacg
     8401 gacgaagcac gccgtttgtt tgcgtctgcg aacgagccga agaagttact gattgttccc
     8461 ggcgccgggc atgtcgtgtt tgccgcggcg ggtgagccgt atctgaatca agtcgaacag
     8521 ttcatgcgcg atgcgatgat taggggacga tagtaaactt tgccgtgggc gtgatcgtct
     8581 cgccggacac gtggtcgtga atgcgaattt gtatttgata ctcgcctggt tcgagcgagc
     8641 gactgtcgat caagcgcgat aacgtcaatc gctggcctgc gtcgttgatc tgtcgccagt
     8701 cttcggtttg tttactcagt tccttgccgt ttttcagtaa cacatactca gcatctgccg
     8761 caggtcgaag cgtcgtctgg tctatcgccg cgttgtagat ttgcaggtaa acaccgactg
     8821 gctgattgcg ccggaagaca ccggaaagat tcgggatgac cttggtggtg ccgatgacga
     8881 atggaccaac cgcagcgccg ccttccatgt tttcgagttt ggcggccaac actacgctcg
     8941 acgcggaaag ccgatcggtc ggaaactccg gcacgacaaa tccgagttgc ttcattcccg
     9001 cggcgccgga ttggatgtcg cgcacgatga cgtcgactcg atagcgtccg ggatcgagta
     9061 tgaaagcgcg cgcgtatgct gacttgcgag tgcgcgtggt gctgagctcc tgggacgtgg
     9121 cggtagtggt tactgaatct tcaaacttac caatctggcg ctcggtcaaa gtgatcaccc
     9181 gtccaaagat attcaagcgc gcagtctcga tgccgccgct gtttgcaaac gagagttgat
     9241 cgttattcgc ctggaccgtg agcactgcga gcacgcggtt gtcggattgt ttgaagtagt
     9301 tgagctgggt ttcgaaattt agcgggttgt cgtcaatgat gggcgtggga gttttggtgc
     9361 cgaggaagtt gcgctcgaac ggctgatcgc ggtcgagtga gcgtatgagg tccagcactt
     9421 cgaaaggtga atcctgcgag cgcatgtagt taacgcgccc gaagccgccg aggtttgcga
     9481 tgcggtcggc gcgcgtttcg agtccggcca gttcggcaaa cgtcggtccc gcgcccggaa
     9541 tgtttatcag cgcgtctttc tcgtcggggt ttctggctaa tcggtactcg cccgatcccg
     9601 tcgggtcgac gaattcgagc tcgacgccgc ttctgacatt tggaatgttt cgataaaacc
     9661 acttctcgaa tggataggtg gacgtcgatc cgccgccttc atatgacggc cgatcgtacg
     9721 aaccaccggc tggatgcgat tcgacctcgt ccggctttcc gaacttgata tagatcctgc
     9781 cgcggtctgt gaggcggcct ggcttgccgg atgagaagtg ctcgtttgcg taggctatgc
     9841 gttcgaaaaa ttcctgctta aactcgttct cttccgtgtc tggatcggga tcgcggctgc
     9901 gccagaagtc ttcgataaac ttctcgcgct cgtcgtctgt agcgagtttc ttccacgcct
     9961 cgcgttcgga ttgagtgaga atcagatcga cgtcgttgat ccagttaacg taagcctttt
    10021 tgggttcctg tttgaccttg cgtggttgtt cttcgggatt gcgctttgtt tgctgggctg
    10081 agattgaagc gatcgagatt aaagcgagca gaagagcggt gacgaatcgt cgcagcatgt
    10141 gggcgtctcc ttagagtcat ccacagatta cacagatttt taggatatct gtgtaatctg
    10201 tggatgaaga aacaacgggg agacgacgtg tgaaaagcct actgagttac tgtgaacttc
    10261 tgcgatggcg ataacgattg tccggtaact cgatctcgga tgcgaatcgc gacttcgtag
    10321 tcacctggtc caagattgcg agtgtcgatc aaacgagcga gtgtcagtcg ctgacctgag
    10381 tcgctcatgc cgcgccagtc ttcagcctgt ttgccaagct ctttaccgtc tctcatcaac
    10441 gagtattcaa cgtcgacaga cggacgcagc gtggtttgat cgattccggc gttgtaaacc
    10501 tgcagataaa cgccgacagg ttggccgcgt ttgtaaatgc ccgagacgtt cgggatcacc
    10561 ttggtcgtgc cgatcacaaa ctggcccacc gccggttgat cgacaaggct ctccagcttg
    10621 gcagcgagaa tcagagttga agttgagagt ttttcctgat cgtacttggg aacggtgaag
    10681 ccgagctgtt tcagaccagt tgcaccggaa gcgacgtcac gcacgattac gtcgactttg
    10741 tatgttccgg gcgcgagcgg cacggctttc tggtatgcag actttcgatc tttcgcttcg
    10801 ctgagctctg tagcggttgc cgtcgtgatt accggatctt cgaagattcc gacgcgacga
    10861 ccagctacgg acgtaatgcg cgcgaagatg ttcatgcgcg cctgttgcaa accgccgctg
    10921 tcctgaaaca ccagatcctt gttttcagcc tgaatcgtga atgccgtgat tacgcgttcg
    10981 tctgactggc ggaagaaatc gattcgcaga tcgaaattga gcgggttctc ctcgacgacg
    11041 cccgtgttca ctgctgtcgc gaggtcgttg aatttgacct gtggcggacg gttgagatcg
    11101 gcgagtagtt gcaaccgcga gaacgggctg tcctgctcgc gttgatagtt ttgattgcca
    11161 acaccgccga tgcccgcaac gcgatctgct ttgctcgtca atccgagttg ctcggacagc
    11221 gttagtcccg caccgggaac catcaacatc gcatcttttt cgttgggatt tcgggcgatg
    11281 cgatactcgc cgctgcctgt tgggtcaacg aactcgatct cgactccgga gccgacaccc
    11341 gccaggtaac gatagaacca gatttcgaat ggatacgtgg tcgtcgaacc gccgccttcg
    11401 tacgacgggc gctcgtaaga tccgcccatc gggtgcgttt cgcgctcatc gggcttgccg
    11461 tacatgatcc agatgcggcc gcgatccgtt ttccagcctg gtatgccgga gctgaagtgc
    11521 tcgttggcat atgcgattcg ctcgtagtac tcctctttga attcgttttc gtcagtatcc
    11581 ggatcgggat cgcgccggcg ccaaaattcc tcgatgaagc gttcacgttc gtcgtcagta
    11641 gccagtttct tgaatgcctt acgttcttcg tcggtgatga tgtaggtaac gtctttgtcg
    11701 agccagtctt tgtaagcctt tttcagttcc ggctttacat tgcgcggctt gttgctggga
    11761 tcttgctgat cctgcgcaaa caggcgcggg ctggcgctgg ggaaaagact cactatcagg
    11821 gccagacgaa gaacaaattt ctggtaagac attatgaaaa actcctgtga tgcttgcgaa
    11881 tcgtgcgcta cggactgctg tcggcttacc tattgaagac cttggattct aagtcgcgct
    11941 tgagaacaca gtcaagatgc cgcggcgttt gggctagttg cctaaaagcc gcggaaacct
    12001 atgtaaatct ggacgtttac gtcgccgtcc gtggtttgtt gaaaatccag gcaatgaaga
    12061 tcgaaatcac gtaaagaacg agcat
//
LOCUS       DASSFQ010000139         6336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1543663, whole genome shotgun sequence.
ACCESSION   DASSFQ010000139 DASSFQ010000000
VERSION     DASSFQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543663"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFH96_04845"
     CDS             complement(<1..465)
                     /locus_tag="VFH96_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET6973327.1"
                     /translation="MSQTDIFRSQAPAPLPPRPISIPTPRETTLRNGLSLVVVEDSRL
                     PLVSYRLAFPVGGAFDPPELPGLTDLLAGLLPEGTQSKTSKEIAEEVARMGASLSAGA
                     TSDYTLVGASALAQFNDLILGLMAEVMLEPSFPENEVELAKQNTKESLRQQRA"
     gene            complement(467..1951)
                     /locus_tag="VFH96_04850"
     CDS             complement(467..1951)
                     /locus_tag="VFH96_04850"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6973328.1"
                     /translation="MADRFHLPSTPIEEFKLSNGLRVVLSPDRAIPVVSIAVYYDVGS
                     RNEREGRTGFAHLFEHMMFQGSENVPKAAHFQYIFNAGGTMNGTTSTERTNYFETLPS
                     NYLPLAFWLESDRMRSLKVTQENLDNQRNAVQEEKRLRYDNQPYVPAFLRMNELIFKN
                     PANAHSTIGSMEDLDAATIDDVKEFFRIYYAPNNAVLTVVGDFDNDEARALAEKYFGD
                     IPAQPVPPPVDVSEPEAVAIREETFHDPLAPAPAFVLGWKIPVRRTPVFYALSLAGTL
                     LFEGDSSRLYQELVKGDESVVSIEGGVDERRGPSALYIFALPKPGQEVNTIREKIFGE
                     IKRIATDGPTEAEMEKLRNSLCNDAVRGRQSTMYRAQRLAEFALYDSDPHLVDSELDH
                     YLAVTADDIKNAVARYVNVDNRVVLDIIPAPTAEEVAASASPQTPGDPHQPAAPPPHV
                     PDQPAAEPKSPVHADVVRTSPSEQPKDPADVPPHTKPGTGPLHP"
     gene            complement(2071..2502)
                     /locus_tag="VFH96_04855"
     CDS             complement(2071..2502)
                     /locus_tag="VFH96_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973329.1"
                     /translation="MSSPYHKEEPPTPERIDDLMLRDDDPDSGEEQNRTASSVRDIAS
                     RIPESLSAIGRDISRTIEKAISAKDDYVVAVKISPEAQEKLEALVQAGVFRNRAEAAG
                     FLIDEGIKTQSALFERVRQKLGEIERLRAELRGMIADNKTV"
     gene            2710..4383
                     /locus_tag="VFH96_04860"
     CDS             2710..4383
                     /locus_tag="VFH96_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6973330.1"
                     /translation="MKRISSHVALVLLVIALVLPSFVVAQTPGVRRASRGATPETIRV
                     QKDFDEALKLVQEQYIDGKKLNFNEVYKSSIIGMLRSLDPHSNYYDREEFDELKTDQR
                     SEYFGIGASIQTYIRGDQADTFITATFENSPAGKAGLRYGDKIVEVDGAAMTGKSSLE
                     VRDKIRGPRGSIVKLTLERAADKRIEKIDIVRDAVPQPSIPDKYMLKPGVGYIDMTRG
                     FNYTTTDELLDAIEQLHGRGMTSLVLDLRNNPGGFLDQAIHVAEVFLRNGQLILTQKG
                     RNGFRDNTYESRNNAPDTTPLVILINENTASASEIVAGAMQDHDRALIVGQTSFGKGL
                     VQSIIPLEYGAGLTLTSAKYYTPSGRLIQRDYSNGGFYDYYTHGAGVGREPKEPAKPA
                     GPEAKTDTGRAVYSGGGISPDEAIGPRTVNLTQRRMLSPAFAFARELVNGRVRGFESS
                     RIAGGINFRHELQPEEFKVTDAMFRAFRDFVAADPAYKVTAAMVDRNRAFVELELRFN
                     MVTAAYGRVIGDRVFIMSNDPQVAKAVDVLPKARDLAMGSEPQKGAKKD"
     gene            4526..4951
                     /locus_tag="VFH96_04865"
     CDS             4526..4951
                     /locus_tag="VFH96_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6973331.1"
                     /translation="MKSRNRPAKGLVNTEELGRAIKRRREELGLSLRDVASETSVSAS
                     TLSRIENGTGKPDADNIARLTTWLDVPMERILSGRDPKAEESKAVVYYPEEPTPEIVE
                     AHLRADKNLTPETASALSELFRVAYTQFSKSDKGQKKRH"
     gene            5051..5608
                     /locus_tag="VFH96_04870"
     CDS             5051..5608
                     /locus_tag="VFH96_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HET6973332.1"
                     /translation="MKALGLRDFARARIDQPLNPFALAHFANLIVIRFDQVEGLSESA
                     REHLLGSASESWSGGACTLPNGMKLVILNPTHGRSRTNATLMEEICHVFLGHQPNRLS
                     IVTKDDRGKVMNRDYRKADEEEAYAVGAAALVPYAWLKRMLLQGRTSTEIGLHFHVSP
                     ELVEYRMKVTRLWHEHKDLSRKSVK"
     gene            5627..>6336
                     /locus_tag="VFH96_04875"
     CDS             5627..>6336
                     /locus_tag="VFH96_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6973333.1"
                     /translation="MKEIASQGLSTTDKLRMLLDITKKISRSLDLQEVLNLVMDTLDS
                     LIPYDAAGIFVLQCQDPELVPEGEEPCTFKSEAVRGYDIDELSDLHLKLGEGFIGSVA
                     VSGQPIISHDVRNDPVYINARDRTRSEMVAPIISNDEVIGVFDLESDELNAYSNDDLE
                     VLMLLASQVAIIIEKVMLHEQLIEKKRLQGQLEVARQVQLELLPSHDPDVPGYDISAY
                     NFPTEEVSGDYYDWVRIY"
BASE COUNT         1396 a         1734 c         1797 g         1409 t
ORIGIN      
        1 cgctcgctgc tgtcgcaaac tctccttcgt attctgcttc gcgagctcga cctcgttttc
       61 aggaaacgaa ggctcgagca tcacttccgc catcaagccg agtatcaaat cgttgaactg
      121 agccaacgct gacgcgccga cgagcgtgta atcagaagtc gcgcccgctg acaaactggc
      181 gcccatgcgc gcgacctctt ccgcgatctc tttactcgtt ttcgattgtg ttccttcagg
      241 caacagaccc gcgagcagat cggtcaggcc cggaagctcc ggtggatcaa atgcaccgcc
      301 cacgggaaac gcgagtctat agctgaccag cggcaagcgg ctgtcttcaa ccacgacgag
      361 tgagagtccg ttacgcagag ttgtttctct cggcgtcgga atggagatcg gtcttggtgg
      421 tagcggcgca ggcgcctggc tacgaaaaat atctgtttga ctcatgttat ggatgtagcg
      481 ggcctgtccc cggcttggtg tgcggcggaa cgtccgctgg gtcttttggt tgctctgaag
      541 gcgatgtgcg aacgacgtcg gcatgcaccg gcgatttagg ctctgccgcc ggctgatcgg
      601 gaacgtgcgg tggcggcgca gctggttgat ggggatcgcc tggtgtctgt ggcgaagctg
      661 atgccgctac ctcttcagcc gtcggagcgg gaatgatatc gagcacgacg cgattgtcta
      721 cgttcacata acgcgccacc gcatttttga tatcgtccgc cgtaacggcg agatagtggt
      781 ccaactccga atcgacgaga tgcggatctg aatcgtacaa cgcaaactcc gccaggcgct
      841 gggcgcgata catggtcgac tgccgtccac gaaccgcatc gttgcagagt gagttccgca
      901 atttctccat ctctgcttca gttggaccat cggtagcgat gcgtttgatc tcgccgaaga
      961 tcttctcgcg aatcgtgttg acttcctggc cgggctttgg cagggcgaag atatacagcg
     1021 cggatggtcc acgacgctcg tcgacgcctc cttcaatcga gacgaccgac tcatcgcctt
     1081 tcacgagttc ttgatacagg cgcgagctgt caccttcgaa gagcaatgta ccggcgagtg
     1141 aaagcgcgta gaaaaccggt gtacgtctta ccggtatctt ccagccgagc acaaaagcag
     1201 gcgccggcgc taacggatcg tgaaacgtct cttcgcgaat cgcgactgct tccggctcag
     1261 aaacatcgac cggcggcggc actggttgcg ccggaatgtc tccgaaatac ttttcagcga
     1321 gtgcgcgcgc ttcatcgttg tcaaagtcgc cgacgacagt gagcacggcg ttgttcggtg
     1381 cgtagtaaat acggaaaaac tctttcacgt cgtcgatggt tgccgcatcg agatcttcca
     1441 tcgatccgat cgttgagtgc gcgttcgcag ggttcttgaa gatcaactcg ttcatgcgca
     1501 gaaacgcagg gacgtacggc tgattgtcgt agcgaagtcg cttctcttcc tgcactgcgt
     1561 tgcgctggtt gtcgaggttt tcctgcgtga ctttcagcga gcgcatgcga tcgctctcga
     1621 gccagaaagc gagaggcaga tagtttgacg gcagagtctc gaagtaattt gtacgttcgg
     1681 tcgaagtggt gccgttcatc gtgccgccgg cgttgaagat gtattgaaag tgcgcggctt
     1741 tgggaacgtt ctccgagccc tgaaacatca tgtgctcgaa gaggtgagcg aagccagtgc
     1801 gtccttctct ctcgttgcgc gagccgacgt cgtagtaaac ggcgatcgaa acaaccggga
     1861 tggcgcggtc tggactcagg acaacacgca ggccgttaga caacttgaat tcttcgattg
     1921 gagttgatgg taagtgaaat ctatctgcca caaaaatttt cccactagaa acaaaggcgt
     1981 gctttaccgt tcgcgaggaa gggtaaagca ctgctttggg gggatgttga taagttaggc
     2041 gcgaaaggcg agcgcccgtt tattgaagct tcagaccgtc ttgttgtcgg cgatcatgcc
     2101 gcgcagctca gctcgcagcc gttcgatttc gccgagcttc tgtctaacac gttcaaacaa
     2161 tgctgactgt gtcttaatgc cctcatcaat caggaatccg gctgcttcag cacgattccg
     2221 gaagacaccc gcctggacca gcgcctcgag tttctcctgc gcttcaggcg agatcttcac
     2281 cgcgaccacg taatcgtcct ttgcggagat cgccttttcg atggtcctac tgatatcgcg
     2341 tccgatggcg ctcagtgatt ccggaatacg cgaggcaatg tcgcggactg acgaagctgt
     2401 tcgattctgt tcttccccac tgtccggatc gtcgtcacga agcatcagat cgtcgattct
     2461 ctcgggtgtc gggggttctt ccttgtgata cggactgctc attttggtat agactccttt
     2521 gccaagtacc gaattctacc tgtccgtctt tacgatagtt tgtcgatgta ggttcattct
     2581 acggagggcg ggaaacacgg tcaacctcgg ggagcacaac cttgctcgac cagtcggcat
     2641 ccgttatatt gcaggcacgg ctaattgata atttcgccgt tagaagtctt caaaaatctg
     2701 aggttcgcag tgaaaagaat ttcatctcac gtcgctctgg tcctactcgt tattgctctc
     2761 gtacttccga gtttcgtcgt tgcgcaaact cctggtgtcc gccgcgcctc gcgcggagcg
     2821 actcctgaga ctattcgtgt ccaaaaggat ttcgatgaag cgctgaagct cgtgcaggag
     2881 cagtatatcg acggcaagaa gctcaatttc aacgaagtgt acaagtcgtc gatcatcggc
     2941 atgcttcgtt ccctcgatcc tcattccaac tattacgacc gcgaagagtt tgacgagctg
     3001 aagaccgatc agcgctccga gtatttcgga atcggcgctt cgatccagac ttacatacgc
     3061 ggcgatcagg ccgacacttt catcaccgcc acgtttgaaa attctcccgc gggcaaggcc
     3121 ggactgcgct atggcgataa gatcgtcgaa gtcgatggtg cggcgatgac gggcaagtcg
     3181 tcactcgaag ttcgtgacaa gattcgtggc ccacgtggat cgattgtcaa actgacactg
     3241 gaacgcgcgg ccgacaaacg catcgaaaaa atcgacatcg tccgcgacgc cgtcccgcaa
     3301 ccatcaatac ctgacaaata catgctgaag cccggtgtcg gttatatcga catgacgcgt
     3361 ggcttcaact acacgaccac agacgagctg ctcgacgcga tcgagcagtt gcacgggagg
     3421 ggcatgacgt cgctcgtgct cgacttgcgc aacaatccgg gcggattcct ggaccaggcg
     3481 attcacgtcg cggaagtgtt tctgcgcaac gggcagttga tactgacgca gaagggccgg
     3541 aatggttttc gtgataacac ctacgagtcg cgaaacaacg cgcccgacac gacgccgctc
     3601 gtgatcctga tcaacgaaaa caccgcctcg gcatctgaga tcgtcgcggg cgcgatgcag
     3661 gaccatgacc gcgccttaat cgtgggccag accagtttcg gtaaaggcct cgtgcagagc
     3721 atcatcccac tcgaatacgg cgccggtctg acgttgactt ccgccaagta ctacacgcca
     3781 tcaggccgtt tgatccagcg cgattattcg aatggcgggt tctatgatta ctacacgcac
     3841 ggcgcgggcg ttggacgtga gccgaaagag ccggcaaagc ctgccgggcc tgaggctaag
     3901 actgacacgg gtcgggcagt ttatagcggc ggtggaatct caccggacga agccatcggt
     3961 ccgcgcactg tgaacttaac tcagcgccgt atgttgagtc cggcttttgc tttcgcgcgc
     4021 gagctggtga atgggcgtgt tcgtggtttc gagtcgagtc gcatcgccgg cgggatcaat
     4081 ttccgtcacg agttgcaacc ggaagagttt aaggtcacgg atgcgatgtt cagagcgttt
     4141 cgtgatttcg tcgcggctga tccggcgtac aaagtgacgg cggcaatggt cgatcgcaac
     4201 cgggcgttcg tcgagctcga gctgcggttc aatatggtga ctgccgctta cggtcgtgtg
     4261 attggcgata gagttttcat catgtcgaac gaccctcagg tggccaaagc cgtggacgtt
     4321 ctgcccaaag cccgtgacct cgcaatgggc tccgagccac aaaaaggcgc aaagaaagac
     4381 tagccacaaa aggcacaaaa agcacaaaag aaaagctgaa ctctgaactt cttttctttt
     4441 gtgctttttg tgccttttgt ggctctgttt cacagacaaa acaaagtctt gcgtctaaaa
     4501 ccgttgctgt gctacgttag gcggcatgaa gtccaggaat cgtccagcca aaggtctcgt
     4561 taacaccgaa gagctcgggc gcgccatcaa acgcaggcgc gaagagctcg ggctcagcct
     4621 gcgcgatgtg gcgagcgaaa cgtccgtctc cgcctcgacc ctttcacgca tcgaaaacgg
     4681 taccggcaaa cctgatgcag acaacatcgc acgactcacc acgtggctcg atgtgccaat
     4741 ggaacggatt ctgagtggtc gcgatcctaa agcggaagag tcgaaagccg tggtctatta
     4801 cccggaagaa cctacgcccg agatcgtcga agctcatctt cgtgccgaca aaaatctcac
     4861 acccgaaact gcatctgcgc tttccgagct gtttcgcgtc gcttatacgc aattcagcaa
     4921 gagcgacaaa ggccaaaaaa aacgtcatta gttttggctg cgtaaatccg cggcctaata
     4981 ctgaatccgg tatactcttc gagtgctgct gggcgatcta cctccaggtg aaaagtggcg
     5041 aaatttcgag ttgaaagctc ttggcctgcg cgacttcgcc cgcgcccgca ttgaccagcc
     5101 attaaaccct ttcgcgctcg cacactttgc aaatctaatc gtgatccgtt tcgatcaagt
     5161 ggagggcttg agtgagagtg cgcgtgagca cctgctcggg tcggcttcag agagctggtc
     5221 cggcggcgcg tgtacgcttc cgaacggcat gaaactggtg atactgaacc cgacgcacgg
     5281 tcgttcgcgc acgaatgcga cgttgatgga agagatttgt cacgtgtttt tgggccatca
     5341 gcccaaccgg ctctcgatcg taacgaagga cgatcgtggt aaggtaatga atcgcgatta
     5401 ccgcaaggcc gatgaggagg aggcctacgc ggtgggcgcc gcggctttag tgccgtacgc
     5461 gtggttaaag agaatgctct tgcagggaag gacttctacg gagattggat tacacttcca
     5521 tgtcagcccc gagctggttg aatatcgaat gaaggttacg cgactctggc acgagcacaa
     5581 ggatctttca cgcaaaagcg taaagtagaa tcccatgacg gaacgtttga aagaaatagc
     5641 atcgcagggt ttgtcgacga ccgacaagct gcgcatgctg ctcgacatca ccaagaagat
     5701 cagccgttca ctcgatctac aggaggtgct gaacctggtg atggacacgc tcgactcgct
     5761 gatcccgtac gacgcagccg gcatctttgt gcttcagtgt caggacccgg agctcgtccc
     5821 ggagggtgaa gaaccttgca ctttcaaatc cgaagcggtg cgcggttacg acatcgatga
     5881 gctttccgat ctgcacctga aactcggcga gggcttcatc ggcagtgtgg ccgtgagcgg
     5941 tcagccaatc atttctcacg acgtccgtaa cgatccggtt tacatcaacg cgcgcgatcg
     6001 cacgcgctcc gagatggtgg cgcccatcat ctcgaacgac gaagtgatcg gcgtcttcga
     6061 tctcgagagt gacgagctca acgcttattc aaacgacgat ctcgaagtgt tgatgctgct
     6121 cgcgtcgcag gtggcgatca tcatcgagaa ggtgatgctg cacgagcagt tgatcgagaa
     6181 gaagcgactc cagggccagt tggaagtcgc gcgccaggtc caactcgagc tgctgccgtc
     6241 gcacgatccg gatgtgcccg gttacgacat cagcgcctac aacttcccga ccgaagaagt
     6301 ctcaggcgat tactacgact gggtgcgaat ctacga
//
LOCUS       DASSFQ010000140         9150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1544056, whole genome shotgun sequence.
ACCESSION   DASSFQ010000140 DASSFQ010000000
VERSION     DASSFQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544056"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /gene="uvrB"
                     /locus_tag="VFH96_04880"
     CDS             <1..1803
                     /gene="uvrB"
                     /locus_tag="VFH96_04880"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET6973334.1"
                     /translation="HNKTLAAQLFQEFRSLFPENKVEYFVSYYDYYQPEAYVPAADVY
                     IEKEAIVNEEIDRLRMSATRALFERRDVIVVASVSCIYGLGDPDAYYGLLFFVEPGSR
                     MSRDVLLQRLVELQYERSDINFQRGTFRVRGDVVEIYPSYQDQAYRIELWGDEIDSIS
                     TIDPLLGEVIEKHTSRVPIYPKTHYVMSRPTIKRAIKTIKEELEWWEPKLIAEGKLVE
                     AQRLHQRTMFDLEMIKEMGFCRGIENYSRHLTGKNPGEPPPTLLDYLPRETLIIIDES
                     HQSVPQVRGMFEGDQSRKRTLVEYGFRLPSALDNRPLNFAEFEARVGQTIYVSATPGP
                     YELTKASGEVVEQIIRPTGLMDPEVEVRPVKGQIDHLLEECRQRAEQHERVLVTTLTK
                     RMAEDLTEYFTEVGVRVRYLHSDIETLERIRILRDLRRGEFDVLVGINLLREGLDLPE
                     VSLVAILDADKEGFLRSEQSLIQTIGRAARNSRGKAILYADRITESMKRAIGETDRRR
                     GIQEIYNRENGITPQTIVKPIEATLITASEADYFKMPSEVEEIEDYSPAKIEATITRL
                     EVEMRAAAKRFEFEKAAELRDRIKVLRERELQVM"
     gene            1877..3319
                     /locus_tag="VFH96_04885"
     CDS             1877..3319
                     /locus_tag="VFH96_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6973335.1"
                     /translation="MQTKSNPEDVQLYLADASFIREGHAERVVFPENVDEVSEIIASA
                     NRDRVPVTISGAGTGTVGGRVAFGGIVLATDKLNRIKSIVHEGDGGFAVVEPAVILSD
                     LQRAVDQEGLLYPPDPTERGCYVGGTVATNASGARTFKYGPTRNYISRLKVVLASGEV
                     LDLRRGEAHADEHGILRLGSIEVKLPHYRRPATRKNASGYFVAPGMDAIDLFIGSEGT
                     LGVICEIEVRLLPKPQSLLSGVVFFADEADVLALVADARTRVDARALEFFDNESLNFL
                     REKYPTVPANAVGAIFFEQETNETNEEAVLAEWQALLDKHHAFTDSWFATNEQDQAKL
                     REFRHQLPVLMNEWFSHYKQRKVSTDMAVPDEAFPGLFRLYKDTLRSSGLRYTIFGHI
                     GDNHVHVNILPRDDAEGARAREFYVQFLKYAASVGGTLSAEHGVGKLKRDYLRFFYTD
                     DQLREMAALKKTLDPNGILGRGNIFSEDLL"
     gene            3363..4532
                     /gene="aroC"
                     /locus_tag="VFH96_04890"
     CDS             3363..4532
                     /gene="aroC"
                     /locus_tag="VFH96_04890"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET6973336.1"
                     /translation="MLRFTTAGESHGRSLIAIVEGLPAGVPVNQELIDNELRRRQLGY
                     GRGGRMKIERDHAEILTGVRHGLTLGSPLTLLIENKDWPNWTEVMASEPRDVAPEKSR
                     RLKRPRPGHADLPGGLKYDVRDLRNILERASARETTARVACGALAKQLLTAFGVEVRS
                     HVIQLGGIPETPLELSWDQLAAIPDDAPLHCADDEAQQKMIELIDETRRQGDTLGGIF
                     EVGARGIVPGLGSHTSWDLKLDGLLAQAVMSIPAVKAVSIGAGAEASSLPGSEVHDEI
                     AYNAETREFIRETNRAGGLEGGITNGEQIRIRGHLKPLSTLRRALRSVDIDTKEEEAA
                     AFERSDVTAVPAAGVIGESMVALVLAGAMREKFGGDSLGEMKRNFDGYREQLRGY"
     gene            4638..5144
                     /gene="def"
                     /locus_tag="VFH96_04895"
     CDS             4638..5144
                     /gene="def"
                     /locus_tag="VFH96_04895"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET6973337.1"
                     /translation="MEIVKWPAPVLDTPADPVTEFDAGLAKLVSNMFETMYAAPGVGL
                     AAVQVGVPKRLFVMDCSGGKDPSQRVALINPEVLRVEGHQNGEEGCLSFPGIFTPVER
                     SVRAIVRAQDLKGEWFELDGMELTARCMLHETDHCDGIVFLDKMSALKREIVKRKIKK
                     LQKAGKWD"
     gene            5223..6815
                     /gene="pruA"
                     /locus_tag="VFH96_04900"
     CDS             5223..6815
                     /gene="pruA"
                     /locus_tag="VFH96_04900"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET6973338.1"
                     /translation="MMISEFRNEPFTDFTKPENAQAMRDALEKVQSQLGREYPLVIGG
                     ERIATGDLLESINPANRTQVIGRFNKATKELANRAVEEAAKAFETWKYVPAADRVAVL
                     FRVAEILRERKHEFSAWMIHEVAKTWAEADADTAEAIDFCEFYGREMLRFAGDHELVH
                     IPGEQNHLDYIPLGVGAVIPPWNFPLAIMAGMTVASVVTGNTVVLKPSSDAPTIAFKF
                     FELLEEAGLPPGVVNFMTGSGAEVGDVVVDHPKTRYIAFTGSKEVGLRINERAARVHD
                     GQIWIKRVVAEMGGKDAIIVADDANLDDAATGVVQAAFGFQGQKCSACSRAIIDARVY
                     DTMIDKIVERTEKLKLADPSDPTTNLSAVINDKAFKTINSYIEKGKSEGGRVVAGGGS
                     DGEQGFFIEPTVIADVKPGATIEQEEIFGPVLAVIKAENYDDALKIANDTQFGLTGAV
                     YSSDEVKLQRARKEFHVGNLYLNRKCTGALVGVHPFGGFNMSGTDSKAGGNDYLLLFL
                     QAKVAAEKVGATKRAEAGSATD"
     gene            6821..7753
                     /gene="fmt"
                     /locus_tag="VFH96_04905"
     CDS             6821..7753
                     /gene="fmt"
                     /locus_tag="VFH96_04905"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET6973339.1"
                     /translation="MARIVFMGTPQAAVPTLRQCVADGHEVVAVWTQPDRPSGRGNKV
                     AFSPVKEFALTQGLSVYQPARIKNDEAKQLFASHHADLAVVVAYGRILPDEFLQAPRR
                     GCINVHFSLLPLYRGAAPTNWAIVNGETETGVTTMFIEPSLDTGPILLQHKTEIGVKE
                     TAPELMQRLAEIGAELLGGTLGRLDELTPRPQHDRDATFAPILTKDDGLIDWSQSAFA
                     IERRVRGFQPWPNAYTSFNGKGLTIWSAEPLASSNPNAVPGEVIVAHGNELLVGCGEE
                     TALRLIDVQPEARKRLAARDFINGTHLKVGDRFG"
     gene            7746..9050
                     /gene="rsmB"
                     /locus_tag="VFH96_04910"
     CDS             7746..9050
                     /gene="rsmB"
                     /locus_tag="VFH96_04910"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET6973340.1"
                     /translation="MVKPSKDISPARASAFNILKQVETGAFSSVLLAAEEPHLQPVDR
                     ALCHELVLGVLRWEFRLDKILKHFSKRSIESLDPPVRIALRLGLYQLRYLTRIPASAA
                     VNESVSLVRAARLSSATALVNAVLRRAIREADYDPAAEVSDPLERIAIQTSHPVWLID
                     RWASAFGVDEAESFASANNVVPPTAFRVVANRGSQSEVLARLNEAGAVLEPSEVVDGA
                     WRVSNAASLLRELSAAGEIYLQDEASQLVAHELEVKSGERVLDLCAAPGGKTTLLADL
                     SGDDAFIVAGDRSASRMETVAATMRSHQLKSIKPVLLDAVDSLPFAAGSFDKVLVDAP
                     CSGTGTLRRNPEIRWRLAPVDIFTLADQQEQILKRALEMVKPGGRLVYSTCSVEREEN
                     EEVVAEVLARGDRFQLLKTIRTWPQREGCDGFFVSIFQCRQD"
BASE COUNT         2020 a         2347 c         2765 g         2018 t
ORIGIN      
        1 cacaacaaga cactcgccgc gcaactcttt caggagtttc gctcgctctt tcccgagaac
       61 aaggtcgaat acttcgtctc ttattacgac tattatcaac ccgaagcata cgttcccgcc
      121 gccgacgtct acatcgaaaa ggaagcgatc gttaacgagg agatcgatcg actgcgcatg
      181 tcggcgacgc gggcgctgtt tgaacgtcga gacgtgatcg tggtcgcgag tgtttcgtgt
      241 atttacggcc tcggcgaccc ggacgcgtat tacggcctgc tctttttcgt cgagccgggt
      301 agtcgcatgt cgcgcgacgt gctgctgcag cggttggttg agctgcagta cgagcgttct
      361 gacatcaact ttcagcgagg cacgtttcgc gtgcgcggcg acgtcgtcga gatttatccg
      421 agttatcagg atcaggcgta tcgcatcgag ctttggggcg acgagatcga ctcgatttca
      481 acgatcgatc cgttgctggg tgaagtaatc gagaaacaca cgtcgcgcgt gccgatttat
      541 ccgaagacgc actacgtgat gtcgcggcct acgatcaagc gagccatcaa gacaatcaag
      601 gaggagctcg aatggtggga gccgaagttg atcgccgaag ggaagttggt tgaagcgcag
      661 cgattacacc agcggacgat gtttgatctc gagatgatca aggagatggg gttttgtcgc
      721 ggcattgaaa actactcgag acacttgacc ggcaagaacc caggcgaacc accgccgaca
      781 ttgcttgatt atctcccgcg cgaaacgctg atcatcatcg atgagtcgca ccagtcagtg
      841 ccgcaggtgc gtggcatgtt tgaaggcgac cagtcgcgca agcgaaccct cgtcgagtat
      901 ggatttcgct tgccgtcggc gctggacaac cggccgttga actttgccga gtttgaagca
      961 cgcgttggac aaacgatcta tgtttcggcg acgccgggtc cgtatgagtt gacgaaggcg
     1021 agcggtgaag ttgtcgagca gattatccgg cctacgggtt tgatggatcc ggaagtcgag
     1081 gttcgtccgg tgaaaggcca gatcgatcat ttgcttgagg agtgccggca gcgtgccgag
     1141 caacacgaac gtgtgctggt gacgacgttg accaagcgca tggccgagga tttgactgag
     1201 tacttcacgg aagttggcgt gcgagttcgt tacctgcatt cagacatcga gactctggaa
     1261 cgaatcagga ttttgcgtga cttgcgacgt ggtgagtttg atgtgctggt tgggatcaac
     1321 ttgctgcgtg aaggactcga tctgcctgaa gtgtcgctgg tggcgattct cgatgcagac
     1381 aaggaaggtt ttctgcgttc ggagcagtcg ttgatacaga cgatcgggcg agcggcgcgg
     1441 aattcacgcg gcaaggcgat cctttacgcc gatcgaatta cagagtcgat gaagcgtgcg
     1501 attggagaaa cggatcggcg tcgcggtatc caggaaattt acaatcgcga gaacgggatc
     1561 acgccgcaga cgatcgtgaa gccgatcgaa gcgacgttga ttacggcgtc ggaagcggat
     1621 tacttcaaga tgccttccga ggtcgaggag atcgaggatt attcaccggc gaagatcgaa
     1681 gcgacgatta cgaggttgga agtcgagatg cgtgcggcgg cgaagcggtt tgagtttgag
     1741 aaagcggcgg agttgcggga tcgcattaaa gtgctcaggg aacgtgagct tcaggtcatg
     1801 tagtgacagg atgaacaaga tttgctcagg atttacggat ctatcctgca catcttgaaa
     1861 aatcctgtaa atcattatgc aaactaagtc gaatcctgaa gatgttcagt tgtatctcgc
     1921 cgacgcgagt ttcattcgcg aggggcacgc ggagcgcgtc gtctttccgg agaacgtcga
     1981 cgaagtttct gaaattatag cgagcgcgaa tcgagatcgc gtgccggtca cgatttctgg
     2041 agcagggacg ggaactgtcg gcgggcgtgt cgccttcggc gggattgtcc ttgcgactga
     2101 caaactgaat cgtatcaaga gcattgttca cgagggcgac ggtggctttg ccgtggtcga
     2161 accggccgtg atcctttcag atctgcagcg cgccgtcgat caggagggac tgctttatcc
     2221 acccgatccg acggaacgcg gctgttatgt cggcggtact gttgctacaa acgcttccgg
     2281 cgcgcgcaca ttcaagtatg gcccaactcg caactacatc tcgcgcttga aagttgtcct
     2341 tgcctccggc gaagtactcg atcttcgtcg cggcgaggca cacgccgatg aacacggaat
     2401 acttcgcctc ggatcgatcg aggtgaaact gccgcattat cgccgtccgg caacacgaaa
     2461 gaacgccagc ggttactttg tggcgcccgg catggatgcg atcgatcttt tcatcggcag
     2521 cgaaggcacg ctcggcgtga tttgcgagat tgaggtccgg ctcttaccaa aaccgcagag
     2581 tctgttgagc ggcgtcgtat ttttcgcaga cgaagcggat gtgctggccc ttgttgccga
     2641 tgcaagaacg cgagtcgatg cgcgtgcgct cgagtttttc gataacgagt cgttgaactt
     2701 cctgcgtgaa aagtatccga cggtgccggc aaatgccgtg ggcgcaatct ttttcgagca
     2761 ggaaacaaac gaaacaaatg aagaggcagt gctggccgag tggcaggcgt tactcgacaa
     2821 gcatcacgcg tttacggatt cgtggtttgc gacgaacgaa caggaccagg cgaaactgcg
     2881 tgagtttcgt catcaactac cggtgctgat gaacgaatgg ttttcgcact acaaacagcg
     2941 caaagtctcg accgacatgg cggtgccgga cgaagccttt cccggcttgt tccgtttata
     3001 taaggacaca ttgcgctcga gcggtctgcg ctacacgatt tttggccaca tcggcgacaa
     3061 ccacgttcac gtaaacatcc ttcctcgtga cgacgcagaa ggtgcccgcg cgcgcgagtt
     3121 ctatgtccaa ttcctaaagt acgccgcatc cgtcggcggc acgctctcag ccgagcacgg
     3181 cgtcggcaag ttgaagcgcg attacctccg ctttttctat acggacgatc aacttcgcga
     3241 aatggcagcg ctgaaaaaga cgctcgatcc aaacgggatc ctgggccgcg gaaacatctt
     3301 ctccgaagac ctgctctaat ctgcttttgt gttttttgtg ccttttgtgc ttttttgtgg
     3361 ctatgcttcg tttcaccaca gccggagaat cacacggacg atcgctgatc gcgatcgtcg
     3421 aaggtctgcc cgcaggcgtc cccgttaacc aggaactaat cgacaatgag ctgcgccgtc
     3481 gtcaacttgg ttatggtcgc ggcggccgca tgaagatcga gcgcgatcac gccgaaatcc
     3541 tgaccggcgt ccggcacggt ctgacgctcg gttcaccact gactctcctg atcgaaaaca
     3601 aagactggcc caactggacc gaagtcatgg cttcagagcc tcgtgacgtc gcgcctgaga
     3661 aatcgcgccg gctaaagcgc ccacgtcctg gccacgccga tcttcccggc ggtctcaagt
     3721 acgacgtgcg cgatctccgc aacatcctgg aacgtgcatc cgctcgcgaa accactgcgc
     3781 gtgtcgcgtg tggcgcgctc gcaaaacagt tgctgacagc ttttggcgtt gaggtgcgca
     3841 gtcacgtgat ccagctcggc ggcattcccg aaacgccact cgagttgagt tgggatcaac
     3901 tcgccgctat tccagacgat gcgccgttgc attgcgctga cgacgaagca caacaaaaga
     3961 tgatcgagtt gatcgacgaa acgcgacgac agggcgacac gttgggtggc attttcgaag
     4021 tcggagcacg tggcatcgtg cccggtcttg gctcgcacac gtcgtgggat ctgaagcttg
     4081 atggattgct ggcacaggcg gtgatgtcga taccggcagt gaaagcagtt tcgatcggcg
     4141 cgggcgcgga agcgagcagt ttgcccggtt ctgaagtgca cgacgagatt gcgtacaacg
     4201 cggagacacg cgaattcatt cgcgaaacca accgcgccgg tggactcgaa ggtggcatta
     4261 ccaacggcga acagatacgc attcgcggtc atctaaaacc gctttcaacc ttgcggcgcg
     4321 cgctccgttc agtcgatatc gacacgaagg aagaagaagc agctgcgttt gaacgttcgg
     4381 atgtgaccgc agtccccgca gccggagtga tcggggaatc gatggtggca ctcgtcctcg
     4441 ccggcgccat gcgcgagaag tttggtggcg atagtttggg cgagatgaaa cgaaattttg
     4501 acgggtacag agagcagcta aggggttact aaatcagaga caggttggac aggatttata
     4561 agattctttc aggatgaaca gaattttcct tttgtgcttt ttgtgccttt ttgtggcaga
     4621 atcaactcat ggctttgatg gaaatagtta agtggccggc gccggtgctc gatacaccag
     4681 ccgatccggt gaccgagttt gacgctggtc tagcgaagct cgttagcaac atgtttgaga
     4741 cgatgtacgc ggcgcccggt gtggggttgg cggccgtgca ggtcggcgtt ccgaagcggc
     4801 tctttgtgat ggattgttca ggcggcaagg atccgtcaca gcgtgtggcg ttgatcaatc
     4861 ccgaagtgct tcgtgttgaa ggacatcaaa atggcgagga aggctgttta tcgtttccgg
     4921 gaatcttcac acctgtcgaa cgcagcgtcc gagcaatcgt gcgcgcacag gatctgaaag
     4981 gcgagtggtt cgagctcgac ggtatggaat tgaccgcgcg ctgcatgctc cacgaaaccg
     5041 atcactgtga tgggatcgtg tttctcgaca aaatgagcgc cctcaaacgc gagatcgtaa
     5101 aacgcaaaat caagaagctt cagaaagccg gtaagtggga ctaattgtag ccgcagattt
     5161 acgcggatga acgcagatct gcgtttatcc gcgttcatcc gcggctaaaa atataggagt
     5221 agatgatgat atccgaattt cgtaatgaac cgtttacaga tttcaccaag cccgagaacg
     5281 cgcaagcgat gcgcgatgcg ctcgagaaag ttcagagtca actcgggcgc gagtacccgc
     5341 tcgtcattgg cggtgaacgc atcgcaaccg gcgacttgct cgagagcatt aatcccgcca
     5401 atcgcaccca ggtgatcggc cgtttcaata aagccacgaa agagctcgcc aatcgcgccg
     5461 tcgaagaagc tgccaaggct tttgaaacct ggaaatacgt tccagccgcg gatcgtgtcg
     5521 cggtgctgtt tcgcgtcgcc gagatcctgc gcgaacgcaa acacgaattc tccgcgtgga
     5581 tgattcacga agtcgcaaaa acgtgggccg aagccgacgc cgacaccgcc gaagcgatcg
     5641 acttctgcga attctacggt cgcgaaatgc tgcgttttgc cggtgatcat gagcttgtcc
     5701 acatcccggg cgaacagaat catctcgact acataccgct cggcgtcggc gctgtcattc
     5761 cgccttggaa ctttccgctc gcgatcatgg ccggcatgac cgtcgcttcc gtggtcactg
     5821 gcaatactgt cgtgctgaag ccttcgtctg atgcgccgac gatcgcgttc aagttcttcg
     5881 aattactcga agaagccgga ctgccgccgg gtgtggttaa ctttatgacc ggttcgggcg
     5941 cagaagtggg cgatgtcgtc gtggaccatc ccaagactcg ttacatcgcg tttaccggat
     6001 cgaaagaagt cggactcaga atcaacgagc gtgcggctag agttcacgac ggccagattt
     6061 ggatcaagcg cgtcgtagcc gagatgggtg gcaaggacgc gatcatcgtt gccgacgacg
     6121 cgaatctcga cgatgcggcg accggagtcg tgcaggcggc ctttggtttt cagggtcaga
     6181 aatgctcagc gtgttcacgt gcgatcatcg acgcgcgagt ttacgacacg atgatcgaca
     6241 agattgtcga gcggactgag aaactcaagc ttgccgatcc atcagatccg acgacaaacc
     6301 tgagcgctgt gatcaacgac aaggcgttca aaaccatcaa ctcttatatc gagaaaggga
     6361 agagtgaagg tggtcgcgtt gtggccggtg gcggttcgga tggtgaacaa ggatttttta
     6421 tcgagccaac agtcatcgcc gacgtgaaac caggcgcgac gatcgagcag gaagagatct
     6481 ttggaccagt gctcgccgtg atcaaagctg agaattacga cgatgcgctc aagatcgcca
     6541 acgatacgca gtttggtttg acgggagctg tttacagcag cgacgaagtg aaattgcagc
     6601 gcgcgcggaa agagtttcat gtgggcaatc tgtacctgaa tcgcaagtgc accggcgcgc
     6661 tcgtgggtgt gcatccgttc ggcggcttca atatgtccgg cactgactca aaggcaggtg
     6721 gcaacgatta tctgctgtta ttcctgcagg cgaaagtcgc ggcggagaaa gttggcgcaa
     6781 ccaagcgagc tgaggcagga tcggcgacgg attagcacgg atggcacgga tagtttttat
     6841 ggggacacct caggctgccg tgcctacctt gcggcagtgt gttgctgatg gccatgaggt
     6901 cgtggctgtg tggacccaac cggatcgacc gtccggtcgc ggcaacaaag tagcattctc
     6961 tccagttaaa gagtttgccc tcacgcaagg tctcagtgtc tatcaacccg cgcggatcaa
     7021 gaacgacgaa gcgaagcaat tgtttgcgtc gcaccacgct gatctcgcgg tggtggtcgc
     7081 gtatgggcga attctgccgg atgaatttct acaagcgccg cggcgcggat gtatcaatgt
     7141 tcacttctcg cttctgcctt tgtatcgtgg cgcggccccg actaattggg ccatcgtcaa
     7201 cggtgagact gaaacgggcg taacgacgat gttcatcgaa ccgtcgctcg atactggtcc
     7261 aattctcctg caacacaaaa ccgagatcgg cgtgaaggaa acagctccgg agttgatgca
     7321 gcgactggcg gagattggcg ctgagctgtt gggcgggacg ctcgggcgtc tcgatgagct
     7381 cacgcctcgt ccccaacacg atcgcgacgc gacttttgcc ccgatcttga cgaaggacga
     7441 cggattgatt gactggtcgc agagtgcatt tgcgatcgaa cggcgcgtgc gaggttttca
     7501 gccgtggccg aatgcttaca cgagtttcaa cgggaaaggc ttgacaattt ggagcgccga
     7561 gccgctcgcg agttcgaatc cgaatgctgt gcctggcgaa gtgatcgtag cccacggaaa
     7621 cgagctgctg gtcggttgtg gcgaagagac ggcgttgcgt ttgattgacg tccagcccga
     7681 agcccgcaag cgacttgcgg cgcgcgattt tatcaacgga acacacctga aggttggcga
     7741 tcgttttggt taagccgtcc aaagatatct cacccgctcg cgcctctgcg tttaatatcc
     7801 tgaagcaggt tgaaaccggc gccttctcct cagttcttct cgccgccgaa gaaccacacc
     7861 tgcaaccggt cgaccgagcg ctttgtcacg agcttgtgct tggcgtgctg cgctgggaat
     7921 ttcgactcga caaaatcctc aaacactttt ccaaacgatc aattgagagt ttggacccgc
     7981 cggtgcgtat tgcgctgcga ctcgggctgt atcaacttcg atatctgacg cgcatacctg
     8041 cttcggctgc cgtcaacgaa tccgtcagtc tggttcgcgc ggcacgtttg agttcggcga
     8101 cagcgctagt taatgcagtg ctgcgacgcg cgatacggga agctgattac gatccagcgg
     8161 cggaagtttc agatccgctg gagcgaattg caattcaaac ttcgcatccc gtgtggttga
     8221 tcgatcgctg ggcgtcggcg tttggcgttg atgaagcgga atcgtttgca agtgcgaaca
     8281 atgtcgtgcc gccgacggct tttcgcgtcg ttgcgaatcg aggcagtcag tctgaggttc
     8341 tcgcaagatt gaacgaagct ggcgcggtgc tcgagccgtc ggaggttgtt gatggagcct
     8401 ggcgtgtttc gaacgctgcg tcgctgttgc gagagttaag cgcggcgggt gagatttatt
     8461 tgcaggacga ggcgtcacag cttgttgcac acgagttgga ggtaaagagc ggcgaacgag
     8521 tgttggatct ttgtgcggcg cctggtggaa agacgacgtt gctggctgat ctgagtgggg
     8581 atgatgcgtt cattgttgcc ggtgatcgtt cagcgtcacg aatggaaaca gtcgcagcga
     8641 cgatgagatc gcaccagctc aagagtatca agcccgttct gcttgatgcg gtcgactcat
     8701 taccgtttgc agccggcagt ttcgataaag tgctggttga tgccccgtgt tccgggaccg
     8761 ggacgttgcg ccgcaatccg gagattcgct ggcgcctcgc gccggtcgat atcttcacac
     8821 tcgccgacca gcaggaacag attttgaaaa gagctttgga gatggtgaaa ccgggtggcc
     8881 ggcttgtcta ttcaacctgt tctgtcgagc gggaagaaaa tgaggaagtc gttgccgagg
     8941 tgcttgcgcg tggcgatcga ttccaactgc tgaaaacgat tcggacgtgg ccgcagcgcg
     9001 agggatgcga tgggttcttt gtttcgatct ttcaatgtag acaggattaa caagattaag
     9061 caggattagc aggatcaatc cagcttgctc cgcgatcaca gcgggtgcta gacttcgcaa
     9121 cttggtgagt ttagttgcca gaattacgtc
//
LOCUS       DASSFQ010000141         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1997322, whole genome shotgun sequence.
ACCESSION   DASSFQ010000141 DASSFQ010000000
VERSION     DASSFQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997322"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /gene="xrtK"
                     /locus_tag="VFH96_04915"
     CDS             complement(<1..493)
                     /gene="xrtK"
                     /locus_tag="VFH96_04915"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase K"
                     /protein_id="HET6973341.1"
                     /translation="MNRRRMLWGAQLAIVLLCALALKQYYSTATANELRWILAPTTLI
                     VELLSGRSFTFESYTGYMSSDRTFVIAVPCAGVNFLITAFVMLALRRLWNGRFESVSW
                     RMLPISAATAYVTTVIANAMRIVIALEIQSRAVEVSWLTENQLHRLEGIVVYFGFLML
                     LFVV"
     gene            complement(490..2478)
                     /locus_tag="VFH96_04920"
     CDS             complement(490..2478)
                     /locus_tag="VFH96_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HET6973342.1"
                     /translation="MRVNFRSFCVALIAVAIAFTCARAQTPADKTLAPYFVVQGDPSV
                     DHLPLKDTRVDISVSGVIADVKVRQIYRNEGSRPINAAYVFPASTRAAVYSMRMQIGD
                     EIIVAKIKEREQAKKDFEQAKEEGKSASLLEQERPNVFSMSLANIMPQEQVEIELRYT
                     ELLVPTDNVYQVVFPTVVGPRYASPQDGNKKQDAFVESPYQHQGEKPASALHISARIA
                     AGVPVYDLTCPSHQIVPQWPSQGVAQLTLDDSDPFQGNRDFVVRYRLAGDQIASGLLL
                     FQGQDENFFLYMAQPPQRVETEAIPPREYIFVVDVSGSMEGFPLNTSKRLLKDLIGKL
                     RPTDLFNVVLFAGDSTVLSPQSLQANPQNIAQAIRLLEQQRGSGGTELLAAIQNAMSL
                     PRQDGISRSVVLVTDGYVSGEQGVFDHIRANLNRCNVFAFGIGGSVNRYLIEGIAKAG
                     MGEPFIVTEEAEAEGVADKFREYIQTPVLTDIQVRSIGFDTYDVNPMRFPDLLAQRPI
                     ILFGKWRGPVAGTIELTGKTGQGEYISRLDVAGAQPDEGNSALRYLWARSRIAELSDY
                     GSSDLAEENIKEVTRLGLKYNLLTRYTSFIAVREVVTNPNGNAQDVKQPLPMPLRVSD
                     LAVGGGAEVGSEPELIWLMAGTLLLATIMILKRRRWFA"
     gene            complement(2701..3981)
                     /locus_tag="VFH96_04925"
     CDS             complement(2701..3981)
                     /locus_tag="VFH96_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET6973343.1"
                     /translation="MEPVIGLDFGTTNSAIAVADDDRQAALASFADGPASTTSFRSIL
                     YFPARDRSSPQKVETQAGPEAINSYLDDDTKGRLIVSIKSYLASRLFTSTSINGRNYT
                     LEDLIAIILRRLRVTAMEQFGTAATQVVLGRPVRFSGAETVADEKLALQRLTAAAELA
                     GFQQVSFELEPVAAAYQYETQLDHDELVLIGDFGGGTSDFTLARLGPGRKKAGRNPVL
                     GTSGVAIAGDTFDSRIMMRLVAPKLGLGSHYISLGKELPVPVWVYSQLSSWHHMFLLK
                     EPKTMAVLREVRNQATEPEKVAALVQIIAENLGYALYRAVEQTKVQLTEKELASFVFS
                     QSIVRLEDSLERWQFESWIQEDIQNIAACVRSLLDQVGVKPTDIDSIFLTGGSSFVPV
                     VRRFFSRTFGANKLRSGEELTTVAKGLALRALEN"
     gene            4026..>4566
                     /locus_tag="VFH96_04930"
     CDS             4026..>4566
                     /locus_tag="VFH96_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973344.1"
                     /translation="MKNMQESPVKIDALLEPLLTATTDEHADELLSQLIAGHAEPVIK
                     GVIRFKLRFNSHQETQRAEADDIHQDVVLQLVMQLQRFRKLPETHPITDVRGMAAIIA
                     HRTCARWMRRQFPERHALKNRLHYLLTRQRGFALWEDSKGKSLAGFAVWQQQKKTAVR
                     NLSDVAAHLRKAQELAETVK"
BASE COUNT         1064 a         1335 c         1204 g          963 t
ORIGIN      
        1 gtacgacaaa caacaacatc aaaaacccaa agtaaacgac gatcccttcg agccggtgca
       61 gttggttctc agtcaaccag ctaacctcca ccgctcggct ctgaatctcc aatgcaatga
      121 caatccgcat cgcattcgca atcaccgtcg tcacataagc cgtagcagca ctgatcggaa
      181 gcatccgcca gctcacactt tcgaaccgcc cgttccacaa ccgtcgcaag gccagcatca
      241 caaacgccgt aatcagaaaa ttcacgcccg cacacggcac cgcaattaca aacgtgcgat
      301 cgctgctcat gtagccggtg tacgactcga acgtaaacga tcggccgctc aacaactcga
      361 cgatgagcgt cgtcggagca agtatccacc gcaactcatt cgcggttgcg gtggagtaat
      421 actgcttgag tgccagagcg cacagcaaaa ctatcgcgag ctgtgcgccc cagagcatcc
      481 tacgccggtt catgcaaacc accttcgtcg cttcaggatc atgatcgtcg ccaacagaag
      541 cgtgccggcc attaaccaaa tcaactccgg ctcgcttccg acttccgcac caccaccaac
      601 cgcaaggtca ctaactctca gcggcatcgg caacggttgc ttcacatcct gcgcgttgcc
      661 attcggattc gtcacaactt cacgaaccgc aatgaacgac gtgtatcgcg tcagcaagtt
      721 gtacttcaaa ccaagccgcg tgacctcttt gatgttctct tccgcaaggt cggacgagcc
      781 gtaatcagac agctcggcta ttcgcgaccg cgcccacagg taacgcaacg cgctgttacc
      841 ttcatcgggc tgagcacctg caacatcgag acgactgatg tactcaccct gacccgtttt
      901 gccggtcaac tcaatcgttc ccgccactgg cccacgccat ttaccgaaca gaatgatcgg
      961 acgttgcgcc agcagatcag gaaatcgcat cgggttcacg tcatacgtgt caaagccgat
     1021 cgagcgcacc tggatgtcag tcaacactgg tgtttgaatg tactcacgaa acttgtcggc
     1081 gacgccttca gcttccgctt cttccgtgac gatgaacggc tcacccatgc cggccttcgc
     1141 gatgccttca atcaggtaac gattgaccga gccaccgatg ccgaaggcga agacgttaca
     1201 acggttcaga tttgcacgaa tgtgatcgaa cacgccctgt tcaccggaca cgtaaccgtc
     1261 agtgacgagt acgacgctgc gagagatgcc atcctgtcgc ggcaaactca tcgcgttttg
     1321 aatcgcggct aacagttcgg ttccaccact gccacgctgt tgctcgagca agcgaattgc
     1381 ctgtgcgatg ttctgcgggt tagcctgcag cgattgcggc gagagcacgg tggagtcgcc
     1441 ggcaaacagc accacgttga ataaatctgt cgggcgcagt tttccgatca agtctttgag
     1501 cagtcgcttt gacgtgttca gaggaaaacc ttccatcgag cccgaaacgt cgacaacaaa
     1561 gatgtactcg cgcggcggaa tcgcttctgt ctcgacacgc tgcggcggtt gggccatgta
     1621 cagaaagaag ttttcatcct gaccctggaa cagcagcagg cccgaagcga tctgatcgcc
     1681 cgcgagacga tagcgcacga cgaagtcgcg attgccctgg aacggatccg agtcgtccag
     1741 cgtcaactgc gccacaccct gactcggcca ctgcggcacg atctgatgtg aagggcacgt
     1801 tagatcgtaa accggcacac ccgcggcaat ccgagccgag atgtgaagag cactcgccgg
     1861 cttctcgccc tgatgttgat aaggtgattc gacaaatgcg tcttgcttct tatttccatc
     1921 ctgtggcgat gcgtagcgtg gtccaacaac ggtcggaaac acgacttggt aaacgttgtc
     1981 ggtcggaaca aggagctcgg tataacgcag ctcaatctca acctgttcct gcggcatgat
     2041 gttcgccaag ctcatcgaga agacgttcgg ccgctcctgt tcgagcagcg acgcactctt
     2101 cccttcttct ttcgcctgtt cgaagtcttt cttcgcctgt tcacgttctt tgatcttggc
     2161 gacgatgatc tcgtcaccga tttgcatgcg catcgagtag acggcggcgc gcgtcgatgc
     2221 aggaaagacg taagcagcgt tgatcgggcg tgacccttcg ttgcgataaa tctgccggac
     2281 tttgacgtcg gcaataacac ctgaaactga gatatcgact cgtgtatctt tgagcggcag
     2341 gtgatcgacg ctcggatctc cctggaccac gaaataggga gcgagcgttt tgtctgccgg
     2401 ggtttgcgcc cgggcgcacg tgaaagcgat cgcaaccgcg atcaaagcaa cacagaaact
     2461 tctaaagttg actcgcatga tttcctctcc gcccttggtg ggcgactaga cgattggacc
     2521 gccttgctgg tcctacttaa aacgcgcaaa tgctaaggcg cggagagctt tgaactaacg
     2581 atacagacca caggtctgca ccgtaccgcc cacagttgag aggaaaacta gccacaaaaa
     2641 ggcacaaaaa gcacaagagt agaaccactc tttttatctt ccttttgtgc gtttttgcgg
     2701 ctagttttcg agagcgcgga gggcgaggcc tttggcgacg gtggtgagct cttcgccgct
     2761 gcggagcttg tttgcgccga aggttcgtga aaagaaacgg cgcacaaccg gaacaaatga
     2821 tgatccaccg gtgaggaaga tgctgtcgat gtcggttggt ttcacgccaa cctggtccaa
     2881 cagcgagcgg acgcaggcag cgatgttttg aatgtcttcc tggatccagg actcaaactg
     2941 ccagcgctcg agtgagtctt cgagtcgcac gatcgattgt gagaacacga agctggcgag
     3001 ctccttttcc gtgagttgaa ctttggtctg ttcgacagcg cgatacaggg cgtagccgag
     3061 attctcagcg atgatctgga ccagcgccgc gaccttctca ggttcggtcg cctggttcct
     3121 cacttcacgc agcaccgcca tcgtcttcgg ctctttcaat aaaaacatgt gatgccagct
     3181 cgataactgc gagtacaccc acacgggcac cggcagctcc ttgcctaacg aaatgtaatg
     3241 cgagccgagg ccgagctttg gcgccacgag cctcatcatg atgcggctgt cgaacgtgtc
     3301 gccggcgatc gcaacgccgg aagtgccgag cacgggatta cgaccagctt tctttctgcc
     3361 tgggccaaga cgagcaagcg tgaagtcgct cgtgccgccg ccgaaatctc cgatcagaac
     3421 taactcgtcg tgatcgagtt gcgtttcata ctggtaagcg gcagcgaccg gctccaattc
     3481 aaacgagacc tgttgaaaac cggccagctc agccgcagcc gtcaaccgct gcaaggcgag
     3541 tttctcatct gcgacggtct cagctccgga aaaccgcacc ggtcgaccca acacgacctg
     3601 agttgcagca gtaccgaatt gctccatcgc agtcacgcgc aaccgccgca gaatgatcgc
     3661 gataagatct tccagggtgt agttgcggcc gttaatgctc gtcgaggtaa acaagcggct
     3721 cgctagatac gatttgatcg agacgatcaa tctgcctttc gtgtcgtcgt cgagatatga
     3781 gttgatagct tctggtccag cctgtgtttc gactttctgt ggtgacgagc ggtcccttgc
     3841 cggaaagtaa aggatggagc ggaagctggt ggttgacgcg ggaccatcgg caaaggatgc
     3901 gagcgccgct tgtcgatcgt cgtcagcgac cgcgatcgcg ctgttggttg taccgaaatc
     3961 gagaccgatg actggttcca tcacgtgtta tcctgcaaac cctgaaatca gatcacactc
     4021 gaacgttgaa gaatatgcaa gagtcgccgg tcaagatcga tgctttgctc gaacctctgt
     4081 tgactgcgac taccgacgag catgcagacg agcttctctc acaactgatc gcgggccacg
     4141 ctgagccagt gatcaaaggc gtcattcgtt tcaaactccg cttcaactcg catcaggaaa
     4201 cgcaacgcgc cgaagctgat gacatccatc aggacgtcgt gctgcaactc gtcatgcagt
     4261 tgcagcgctt tcgtaagctt cccgaaacac atccgatcac agacgtacgt gggatggcgg
     4321 cgatcatcgc tcatcgcact tgtgcgcgtt ggatgcgacg ccagtttccc gagcgacacg
     4381 cgctcaaaaa tcgcttgcac tacttactga cgcgccagcg cggcttcgcg ctctgggaag
     4441 atagtaaagg aaaatcgtta gccggtttcg ccgtgtggca acaacagaag aagactgctg
     4501 tgcgcaatct ttctgacgtt gcagctcacc tgcgtaaagc acaggaactc gcggaaacag
     4561 tcaaga
//
LOCUS       DASSFQ010000142         4883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_211007, whole genome shotgun sequence.
ACCESSION   DASSFQ010000142 DASSFQ010000000
VERSION     DASSFQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211007"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFH96_04935"
     CDS             <1..444
                     /locus_tag="VFH96_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973345.1"
                     /translation="TDNNGFLDTIEYDYDGDRTIDLRVSLLDYKTASNPHPDVVQLID
                     THKEGWKGLNVLFGQIASQSFQEGLMVYHAAWKRGLTTPEIDKLASASAIGERYDHGY
                     WLKEKIFRLIRAKVKGTTLEKDLTRLYYTGQFAEYARRISEVPAQ"
     gene            578..1747
                     /locus_tag="VFH96_04940"
     CDS             578..1747
                     /locus_tag="VFH96_04940"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET6973346.1"
                     /translation="MPQNRVSIFDTTLRDGEQSPGCSMNLTEKVRMAKKLQDLGVDVI
                     EAGFPIASEGDFAAVRAVAAQCQDVKVAALCRTSELDVARAADALKFAVHPRIHTFCA
                     TSDIHLEHKLKKTRAEVIQMTRAAVSQARSYAEDVEFSAEDATRSDIDYLCEVLDVAV
                     EAGASVLNVPDTVGYTTPLEYMHLVQQVRDRVVRDRDVIISVHCHNDLGLAVANSLAA
                     IEAGARQVECTINGIGERAGNASLEEIVMALHVRADRFQFETSINTAELYPTSQMLSS
                     IVGFDVQPNKAIVGRNAFAHEAGIHQHGVINNPLCYEIMTPESVGVPTNSLVLGKHSG
                     RHALSLRYAELGYDVTSAELEMAYRSFTDLADKKKRIYDQDLISLLADRVVGAAA"
     gene            1790..2899
                     /gene="leuB"
                     /locus_tag="VFH96_04945"
     CDS             1790..2899
                     /gene="leuB"
                     /locus_tag="VFH96_04945"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET6973347.1"
                     /translation="MRIKITALPGDGIGPEVTKEAITVLQTVAAIHGFRFDFEEHAVG
                     GVAIKQFGSPLPRTTLDACLASDAVLLGAVGSPEFDRMPANQRPEAGLLLLRSALGGY
                     ANLRPAISYSSIVACSPIRPEVARGTDILIVRELLGGLYFGEPRGIDTSNGTAVGINT
                     MRYSVEEIERVARVAFELARKRRGKVTSVDKANVLETSQLWRQTVTRVSQDYPDVTLD
                     HLYVDACAMHLIANPRRFDVLLTENLFGDILSDEAAVISGSLGMLASATIGGEVGLYE
                     PVHGSAPDIAGQGSANPCGAVASAAMMLRHSFGLEQEACDIELAIKDVLEAGYRTPDI
                     AEGTGGYVVTTGEFGELICQAVTEIADMRHAYHAV"
     gene            2914..4317
                     /gene="leuC"
                     /locus_tag="VFH96_04950"
     CDS             2914..4317
                     /gene="leuC"
                     /locus_tag="VFH96_04950"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF004016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET6973348.1"
                     /translation="MTTLFEKLWQAHVVREAQGEPTLLYIDLHLIHEVTSPQAFDGLR
                     QANRKVRRPDRTFGTVDHNVPTTDRSLPIADATAAKQVETLRRNCEEFGIQLFDIHSP
                     EQGIVHVIGPELGLTRPGMTIVCGDSHTSTHGAFGSLAFGIGTSEVEHVLATQCLPQN
                     KPLTMKVQTRGLLPEGVTAKDLALGIIGMIGTDGATGCAIEYAGEAVRALSMEGRMTL
                     CNMSIEAGARAGMIAPDETTFAYLKGRRFAPDGDEWDQAVNYWRTLKSDDDALFDRVV
                     EIDATQLEPFVTWGTSPGMVVPVTSFVPDPAEATSAADRQAAERALEYMALKPRMNIQ
                     DISIDRAFIGSCTNARIEDLRAAARVVKGYHVSSSVSAMVVPGSQDIKKAAESEGLDR
                     IFHEAGFEWREAGCSMCLGMNADILQPGERCASTSNRNFEGRQGRGGRTHLVSPMMAA
                     AAAIKGHFTDVREWEFK"
     gene            4392..>4883
                     /gene="leuD"
                     /locus_tag="VFH96_04955"
     CDS             4392..>4883
                     /gene="leuD"
                     /locus_tag="VFH96_04955"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET6973349.1"
                     /translation="MKAFTKHTGLVAPLDRANVDTDQIIPKQFLKRIERTGFGEFLFY
                     DWRYLPDGRPNPSFVLNEPRYQGASILVADKNFGCGSSREHAPWALGEFGFRVIIAPS
                     FADIFANNCFKNGMLPIALEKEQVQEIMRRAQEQESYELTVDLERQTIGDDISFSVGD
                     FQRY"
BASE COUNT         1107 a         1270 c         1415 g         1091 t
ORIGIN      
        1 acggacaaca acggattttt ggacacgatt gagtacgact acgacggcga tcgcaccatc
       61 gatctgcgtg tctcgctgct tgactacaaa actgcgagca atccgcatcc ggacgtcgtg
      121 cagctgatcg atacccacaa ggaaggctgg aagggtctca acgttttgtt tggccagatc
      181 gcgagccagt catttcagga aggattaatg gtttaccacg cggcgtggaa acgaggatta
      241 acaacgcccg aaatcgacaa actcgcctcc gcctcggcca tcggcgagcg ctacgatcac
      301 ggttactggt tgaaggagaa gatcttccgc ctgatccgag ccaaagttaa ggggactact
      361 ttagagaaag atctcacccg tctctactac accgggcagt tcgcggaata tgcccgtcgc
      421 atctctgaag tcccagctca gtaatccaca gattacgccg attatctgtg taatctgtgg
      481 ataaaaacaa tctatattcc tataaagatt atagctttga ctattggccc aaccctccgt
      541 agcatccctg tttgcttgcc cacaggaggg gaaatccatg ccacaaaaca gagtcagcat
      601 ttttgatacg accttacgcg acggcgaaca gtcgcccggc tgctcaatga acctgacaga
      661 gaaggtccgg atggccaaaa agctccagga tctcggggtc gacgtcattg aagcaggctt
      721 tccgattgcc tcggaaggtg acttcgccgc tgttagggct gtcgcggccc agtgccagga
      781 cgtcaaagtt gctgcgctct gccggacttc ggaactggac gtcgctcgtg ccgccgacgc
      841 tttaaagttt gcggttcatc cgcgcattca caccttctgt gcaacctctg acattcatct
      901 cgaacacaaa ttaaagaaaa cgcgtgctga agtgatccaa atgacgcggg ccgcagtgag
      961 ccaggcacgc agttatgccg aagatgttga gttttccgcc gaggacgcga cgcgcagtga
     1021 catcgattat ctctgtgagg ttctcgatgt cgcagtcgaa gccggtgctt cagtgctcaa
     1081 cgtgcctgac accgtcggct acaccacacc actcgaatac atgcatttgg tccaacaggt
     1141 ccgcgaccgt gtggttcgcg atcgcgacgt catcatcagc gttcactgtc acaacgatct
     1201 cggattggcg gttgcaaaca gtctggcggc gattgaagcg ggtgcacgac aggttgagtg
     1261 cacgatcaac ggcatcggcg aacgtgcggg caacgcgtcg ctcgaagaga tcgtgatggc
     1321 gttgcacgtg cgcgccgatc gttttcagtt tgagacgagc atcaacaccg ctgagctgta
     1381 tccgacgagc caaatgcttt cgagcattgt cggctttgac gtgcagccga acaaagcgat
     1441 cgtcggacgc aacgctttcg cacacgaggc cggcattcat caacatggcg tgattaacaa
     1501 tccgctttgt tacgagatca tgactcccga gtcagtcggc gtgccgacaa attcgctggt
     1561 gctgggtaag cactccggcc gccacgcact ttcgctgcgt tatgcagaat tgggttacga
     1621 cgtcacgtcg gcggagctcg aaatggcgta tcgcagtttt actgatttgg cagacaagaa
     1681 gaaacgcatc tacgaccagg atttgatttc gctgcttgcc gatagggttg ttggcgctgc
     1741 agcgtaacca aatattagcc acggataaca cggatttaaa aaaggattta tgcgaattaa
     1801 gatcacagca cttcccgggg atgggattgg accagaagta acgaaagaag caatcactgt
     1861 tctgcaaaca gttgcggcga ttcacggctt tcgattcgat tttgaagaac acgccgtcgg
     1921 cggtgtggct atcaaacagt tcggatcacc gttgccgcgc acgacgcttg acgcgtgtct
     1981 cgcttccgat gcggttttgc tcggcgccgt cggatcaccg gaattcgatc gaatgccggc
     2041 gaatcaacgt cccgaagcag gactgttgct gctgcgaagc gcacttggcg gttacgcaaa
     2101 ccttcgtccc gcaatttcat attcatcgat tgtggcctgc tcgcccatac gacctgaagt
     2161 cgcgcgtggc actgacattc tcatcgtgcg cgaattgctc ggcggcctct actttggcga
     2221 gccgcgtggt atagacacaa gcaacggcac tgccgtcggt attaacacga tgcgttactc
     2281 agtcgaggag atcgaacgcg tcgctcgtgt cgccttcgaa ttagctcgca aacgtcgtgg
     2341 caaggtaacg tccgtcgata aagcaaacgt gctcgagacg tcgcaactct ggcgtcagac
     2401 cgtgactcgc gtttcgcagg attatcctga cgtcacgctc gatcatctct atgtcgacgc
     2461 gtgtgcgatg cacttgattg cgaatccgcg tcgcttcgat gtgttgttaa ccgagaatct
     2521 ttttggcgat attctttccg acgaagccgc cgtgatcagc ggctcgctgg gaatgctggc
     2581 gtcagcaaca atcggcggag aagtcgggct ttacgaaccg gttcacggtt cggcgcccga
     2641 catcgccggt caaggcagcg cgaatccgtg cggcgcggtt gcgtcggcag cgatgatgtt
     2701 gcggcattca ttcggactcg aacaggaagc gtgtgatatc gaactggcga tcaaggacgt
     2761 gctcgaagcc ggttatcgca cacccgacat cgccgagggc accggcggtt acgtcgtcac
     2821 gacgggcgag tttggcgaat tgatctgtca ggccgtgaca gagatcgccg acatgcgtca
     2881 tgcttaccat gcagtctgaa aagtttttgt agcatgacga cattattcga gaaactctgg
     2941 caagcgcacg ttgttcgtga agcgcagggc gagcccacgc ttctctacat cgatctacat
     3001 ctcattcatg aagtgacatc gccgcaggcg ttcgacggtt tgcgtcaggc aaatcgcaaa
     3061 gtgagacgcc cggatcggac tttcggcacc gtcgatcaca acgttcccac aacagatcgc
     3121 agtcttccga tcgcagacgc aacggcggct aagcaagtcg agacgctgcg tcgcaactgc
     3181 gaagagttcg gcattcaact cttcgatatt cattctcccg aacaaggcat cgttcacgtt
     3241 attggaccag agcttggttt gacgcggccg ggcatgacga tcgtctgtgg tgatagtcac
     3301 accagcactc acggcgcgtt tggatcgctc gcgtttggca tcggcacgag cgaagtcgag
     3361 cacgttttgg ccacgcaatg tctgccacaa aacaaaccgc tgacgatgaa agtgcaaacg
     3421 cgcggtctgt tgcccgaagg tgtgacggcg aaagatctgg cgctcggcat catcggcatg
     3481 atcggcactg acggcgcaac gggttgcgct atcgaatacg ccggcgaagc ggtgcgcgcg
     3541 ctctcgatgg aagggcgcat gacgctctgc aacatgagca tcgaagcagg cgcgcgcgca
     3601 ggcatgatcg cgcccgatga aacgacgttt gcatatctca aaggccggcg ctttgctcct
     3661 gacggtgacg agtgggatca ggctgtcaat tactggcgga cgttgaagag tgacgatgat
     3721 gccttgtttg atcgagtagt tgagattgat gccacgcaac tcgagccgtt tgtgacgtgg
     3781 ggcacaagtc cgggcatggt cgtgccggtg acgagttttg ttcctgatcc cgcggaagcg
     3841 acgagcgcgg ctgatcgaca agcggccgaa cgagccctcg agtacatggc actaaagccg
     3901 cgcatgaata ttcaggatat ctcgatcgat cgagcgttca ttggctcgtg cacgaatgct
     3961 cgcattgaag atttgcgagc ggcagcgcga gtggtgaagg gttatcacgt gagttcctca
     4021 gtgagtgcga tggttgtgcc tggttcgcag gacatcaaaa aagcggcgga gagtgaagga
     4081 cttgatcgca tttttcacga ggcgggtttt gaatggcggg aagctgggtg ttcgatgtgt
     4141 ttgggcatga acgccgacat tttgcagccg ggcgagcgtt gcgcgtcgac gagcaatcga
     4201 aactttgaag gacgacaggg acgcgggggg cgtacacatc ttgtcagtcc gatgatggcg
     4261 gcggcagcgg cgatcaaggg acacttcacc gatgtgcgcg aatgggagtt taaatagata
     4321 aagatttaca agaatttctc aggatgtgca tgtaaatcct gaaaacatct ggtaaatcct
     4381 gtccaactat aatgaaagca ttcacgaaac atacgggcct tgtggcgcca ctcgaccgcg
     4441 cgaatgtcga tacggatcag attattccga agcagttctt gaagcgcatt gagcgcactg
     4501 gctttgggga gtttctgttt tacgattggc gttacttgcc tgacggtcga ccgaatccgt
     4561 ctttcgtgct gaatgagccg cgttatcaag gcgcgtcgat cctggttgcg gataagaatt
     4621 ttggttgtgg ctcgtcgcgt gagcatgcgc catgggcgct cggtgagttt gggtttcgag
     4681 tgatcattgc gccgtcgttt gccgatatct ttgcgaacaa ttgcttcaag aatggcatgc
     4741 tgccgatcgc gctcgagaag gaacaggtgc aggagatcat gaggcgcgcg caagagcagg
     4801 aaagctacga gctgactgtc gatctcgaac gccagacgat cggggatgac atctctttta
     4861 gtgtcggaga ttttcagcgg tat
//
LOCUS       DASSFQ010000143        22767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1391785, whole genome shotgun sequence.
ACCESSION   DASSFQ010000143 DASSFQ010000000
VERSION     DASSFQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22767
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391785"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFH96_04960"
     CDS             complement(<1..713)
                     /locus_tag="VFH96_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973350.1"
                     /translation="MPETENHPLNIICLATYFKGADFIRECKAHGCHVILITKEKVLH
                     EDWPRESLDDLIAVPNDAGPPLFIDLLAFLGRKMKIDRVVALEEFDVVTAALMREHLC
                     LPGLSSSGAKVFRDKLSMAVHSRRAGIDVPEFVPLVNVEEVDDFMQRVPGPWVIKPRS
                     DVSAIGIRKVSEPEEVRRAMHEMNERQNLRERASYYVLARFVAGEVFHVDSLVNDGKV
                     MFAGTNQYGRPPMQVAHQGG"
     gene            789..2354
                     /locus_tag="VFH96_04965"
     CDS             789..2354
                     /locus_tag="VFH96_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HET6973351.1"
                     /translation="MIIKGPYPDISIPETALTPFVLHRAKELGDKPALIDGPTGRTFS
                     YQQVADSISIVAHNLAARGFRKGDVCGILSPNCPEYGIAFHAVATLGGIVTPINPLYT
                     RYEVGHQLKDSGARFLITVPACAEKVLEADNKAGLDELIVFGTLPGATPFDELLVDNG
                     RAEQVEINPREDLIALPYSSGTTGLPKGVMLTHYNLVANLRQMEGLCYFYDTDTLIGV
                     LPLFHIYGLVVVLNMGLYSGATVVMMPRFELESFLKTAQDYEVTLAHLVPPILLALAK
                     HPVVDNYKLPKLHTIFSGAAPLGEDLTRACMERLGCSVRQGYGMTETSPVTHSSPAPP
                     LPVKFGSVGVPAPNTECKVIDLETGEQLGSGQKGEVCMRGPQIMTGYLNKPEATAQTI
                     DPEGWLHTGDIGYYDDDGHFFIVDRAKELIKYKGFQVPPAELEAVLLTHPCVADAAVI
                     PYPDEEAGEVPKGIIVLREPAEADAIMEFVAERVAPHKRIRHLEFVDKIPKSPSGKIL
                     RRVLVEMDRTKVF"
     gene            2489..3541
                     /locus_tag="VFH96_04970"
     CDS             2489..3541
                     /locus_tag="VFH96_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6973352.1"
                     /translation="MHSRRLHLVLASILLAAFAVAAHAQTPADVWPEWETLSPENEEF
                     TVLMPKNPTTESTTFPYHKMEMKARLYMSTSSAGPVLAIASLSGIKSDPAQYTEFARF
                     NSYIDAFKTFFPVKVRSKETLVKMVLVSSRPFHGHTGRSYKITLGDLNGSVNAFVTRK
                     RFYAIVSLNTKKDEALEEKFLSSFVLPERQNEPPKTVASTNPDQQNPNPAEKPLDPNT
                     QNTVQSEGDATSPATPNNAETPAAPTTPTNTRQGPGGEAGKKGPINGGMLNGKAIYFP
                     VPEAPAGDANGVVIVAILVDEQGSVVDARAVSGPQHLQPTAVAAARLARFSPTLLMGE
                     PVRVSGTLSYNFVKSN"
     gene            3649..5085
                     /gene="lysA"
                     /locus_tag="VFH96_04975"
     CDS             3649..5085
                     /gene="lysA"
                     /locus_tag="VFH96_04975"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET6973353.1"
                     /translation="MSTGIATSCPAWSIPGYLEVRNDHLIVNGVDTLELVKEFNSPLF
                     VFSEARIRDNIKRLQLAADAVDRPIKFCYASKANSNMSILKVVVESGIDIEVNSGGEL
                     FKALRIGFHPRQIIFNGTSKSDDELDDAVRAGIYAINVDSIYEIDLVEQAVERLRAHG
                     ENVAPARVTLRLVPEIGTRSHLGLQTALLTSKFGISSSEVLAAFRRGLEKPDLVHVCG
                     IHIHVGSQTPDVEPFAEAFKSMWEHLVTIHRETGHTLEHINLGGGIPVNYLRDRSQAD
                     QLPEHERDMLGAELEPSAVLKEALRVARESARAADAEHLLDQVTILLEPGRSIIADAG
                     LVLTTVRNIKSRPETGDVWLLTDAGYNIMLSMNNYKWYYHLISASRAGAPYAHDYKVA
                     GPLCDSGDVYFDIEREGRLPDYRKLPEDVGPGEVLALLNCGAYSLAQMFHYNGRPLPA
                     AVLIRGDKSVSLIRRRDSYDDLIANDIF"
     gene            5112..6527
                     /locus_tag="VFH96_04980"
     CDS             5112..6527
                     /locus_tag="VFH96_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6973354.1"
                     /translation="MQTQHTQLIRGLGLIAAISVNVANIIGTGVFLKGRVMTCNVGTP
                     GKALIVWVIAGVLSLAGALTYAELLAMMPRAAGEYGIIRDAYGRPLSFIYGWAQFLIA
                     RTASAAALAVGFAIFLNDLVKGALDTVYFKYTFPGGHVVSFGRLQLVALTAIIVTTLI
                     NCAAVRVSGGVASVLTGMKLVLLLGIGFGAFFYSGGDWSHLGMANIGGACEGVAITTG
                     GMAGFAAAMLGALWAYDGWNNITFLAGEVKNPGRNLPLALIGGGLVVMGLYLFVNVSY
                     FHVLSPTEMASVPASSSVAAEVVKRLLGAGAVTLMAAAMMTSSFGALHASLLATARIP
                     YALAKDGLIIKSLAHVSPHTHVPVRALMVQMFWACIVALSGSYDTITDYAIFALTLFY
                     ILVAASIFLFRRRMPDAERPYRTWGYPVVPVVFICVSVWLIFQTIRNTPQQSAIGLGL
                     IMLGLPVYWLISRSRKSTKEI"
     gene            complement(6593..7456)
                     /locus_tag="VFH96_04985"
     CDS             complement(6593..7456)
                     /locus_tag="VFH96_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973355.1"
                     /translation="MHIYLLIVWLVTYGQPATLATIKDKSISESSGLVASRTMPGAYW
                     THNDSGDGPFIYAFDTRGDSFGTFRVSGAEARDWEDIAAGPGPQPNRSYLYVGDIGDN
                     DAVRPEVIVYRVAEPALSTSTRKFTKSRPGSTEPAEAIRLKYPDGKHDAEALLVHPRT
                     GNLYIVTKVLIANPVVYEAIAPFTSGQSITMRRVGEPRVPSLFGGAITGGSISPDGRR
                     VALCDYFSGYELVLSAGASNFDDIWKQKMISFDLGKRKQGESITYRLDGKAFLATSEG
                     KQSPLIQVLRN"
     gene            complement(7535..9007)
                     /locus_tag="VFH96_04990"
     CDS             complement(7535..9007)
                     /locus_tag="VFH96_04990"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET6973356.1"
                     /translation="MIDTQRIAELTDAKLSGEGTRIVTDVSHDSRRVGAGTLFAAVAG
                     ALFDAHKFVPQVMEQGAAGVLSERPAPEGFNGAWLQVEDIRRAMALAAAAVQNHPSRE
                     LQLVGITGTNGKTTTAYLIASIPEEAGELVAMTGTVEYRLGKERFKADRTTPEAPDMQ
                     RLLRRAVEIGCRTAVMECSSQAMDFHRCDELEYAVAVFTNLTRDHLDYHKTMENYWYA
                     KQRLFDGRLGTRPRHSVINVDDPYGVELAASLEKDGMRVIRYAVNSDADVTARDPEFS
                     LSGMRFRLKTPAGERDFHSPLVGPPHIYNTLAAVASGLALGYSLEVVTAALEKCTGAP
                     GRFERVVHDGGFSVVVDYAHSDDALLNVLRTAREVVQGKIITVFGCGGDRDRTKRAPM
                     GEAAGSLSDVVILTSDNPRTEDPEQILCDAEEGIQRTGKPYRKIADRTEAIHQAIAEA
                     QAGDLVLIAGKGHEDYQIIGRETFHFDDKEVARAALLQRS"
     gene            complement(9004..12213)
                     /locus_tag="VFH96_04995"
     CDS             complement(9004..12213)
                     /locus_tag="VFH96_04995"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973357.1"
                     /translation="MLKSLSLCLALALTLSAVHAQNGAPPKPPDLLKALQYRSIGPYR
                     GGRSAAVTGVPSQPFVFYYGATGGGVWKTTDGGINWESVSDGSVFGTGSVGAIAVADS
                     DPNTVYVGMGESPIRGNVSHGDGVYKSNDAGKTWKRVGLEDTRQIPRIRVHPKNPDLV
                     YVAALGHVWGPNEQRGVFRSKDGGKTWEKILSRGSKAGAIDLILDPTNPNIIYAGFWE
                     VSRKPWTLESGGPGGGIFKSTDGGDTWNELTKNPGLPKGTVGKIGIAVSPANPDRIWA
                     IVEAEDGGVFRSDNGGSTWSKVNEERRLRQRAWYYTRIYADPKNADTVYILNTGFYRS
                     NDGGRTYTGIGVPHGDNHDLWIAPDDPNRMIESNDGGSNVSYNGGRSWTEQDQPTAQF
                     YRVALDNDFPYNVYGAQQDNSTVRIASRTTEGGIGTSDWYDVGGGESGWIAPSPKDSN
                     IVFAGSYGGLTTRYDKRTGQLRDVNPYPNNPMGSGADVLKYRFQWNFPLVFSPHDPNK
                     LYVAANVLFMSMDEGNSWQIISPDLTRNDKTKQASSGGPITKDNTSIEYYGTIFTVME
                     SPVQAGTIWAGSDDGLVHVSRDGGKNWSNVTPPKDIMPEWIQINSIDASPFDPGTAYV
                     AATMYKFDDNKPYLYRTSDYGKTWKKITTGIPDTTFTRVIREDPNKRGLLYAGTETGM
                     YVSFDNGEHWQSMQLNLPIVPITDLAIQKREKELVVATQGRAFWILDDLPVLHEMMDT
                     GGFSAAGETRLYKPKESYRMPGGGFPLGPTATLGRNPANGVVVYYSLKAKPTADVDLE
                     FLDSAGKSIRKFTAKAKGEGAAAAGPPTAPPGEGGFFGGGGAPPVSTDVGLNRFVWDT
                     RYPDAVRFPGMILWAGETRGPKLLPGTYQVKLTVEGKSFTQAFEIKADPRLSTTPADY
                     AKQLELNLKIRDKLSETHNAIIQIRDVRKQVEDLLKRVGGQPNFKVVNDAGTALNKNL
                     TAIEEALYQTKNQSSQDPLNFPIRLNNKLAALGGVVASAEASPTAQSYAVYDEVVAQI
                     DAQLQKLGQLMRTDVPAFNQLVKDQNIPAVVVKPQSNNP"
     gene            complement(12297..13295)
                     /locus_tag="VFH96_05000"
     CDS             complement(12297..13295)
                     /locus_tag="VFH96_05000"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET6973358.1"
                     /translation="MKHIAVIGAGSWGTALAVIAARAGHSVRLWSRNDAVVSSINEQR
                     VNSRYLSATSIPDNVTATGDVEAALQDASMVLLAVPSHAARETLLALTSHLHEAAIIV
                     NVAKGIEIESGKRISEIAKEVVAGTHPFVTLSGPSFAKEVVAGHPTAIVAASKDIAAA
                     RAVQNDLSFENLRIYTNADVVGTEIGGSVKNVMAIAAGMADGLGFGANSVAALITRGL
                     AEITRLARREGAQMETLMGLAGLGDLVLTCTGNLSRNRFVGAELGKGRTLAEITAELS
                     EVAEGINTARAVKKLADRASLEMPITNEVNAVLYDGKPARDAVSELMSRPLREERN"
     gene            complement(13299..13889)
                     /gene="plsY"
                     /locus_tag="VFH96_05005"
     CDS             complement(13299..13889)
                     /gene="plsY"
                     /locus_tag="VFH96_05005"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET6973359.1"
                     /translation="MIYTMDKILLVVAAYLLGSIPFGYLIVRGKIGADVRETGSGGTG
                     ATNVSRRAGKAAGVLTLLLDAAKGCVAVLIAKAFGGDDWVIAVAAIAALVGHIFPVWL
                     GFRGGKGVATGVGIFLVLAPIALLCAGVVFVALVLLTRYVSLGSITAAILIPVLVWVQ
                     SDSRPLLVAAIVGAALIVFAHRGNIQRLLSGTESRI"
     gene            complement(13940..15184)
                     /locus_tag="VFH96_05010"
     CDS             complement(13940..15184)
                     /locus_tag="VFH96_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET6973360.1"
                     /translation="MFESLSAEIIAIGSELLAPDRTDTNSLWLTEQLNRLGIEVKLKT
                     IVGDDDARLEEAIGDAVRRSKVVITTGGLGPTEDDITRKITARALRKRLLLDEVVLAE
                     IRQRFQSFGVTMPERNSRQAMVIEDAEVLPNPNGTAPGMFIDHQGTAIVLLPGPPREM
                     RPMFENHVAERLAGRAGSLRVVRRMLRVAGMGESAVDEKIAPVYTQYDNPVTTILFNH
                     SEIEIHLTARGRTEKEASELLDRLSAQIEERLGNAVFSFAGETMEEVVGLKLSLGGYT
                     LAVAESCTGGLLAQRITDVPGSSKYFIGGVIAYANEVKTKELDVEPMLLMEHGAVSAP
                     VAEAMAEGVRKRIGTDFGLSITGIAGPGGGTEDKPVGTVFIALADDVKTEHRRLNLPG
                     DRNLIRWRASQAALDMLRRRLL"
     gene            complement(15191..15916)
                     /locus_tag="VFH96_05015"
     CDS             complement(15191..15916)
                     /locus_tag="VFH96_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973361.1"
                     /translation="MTGAGKLTTKVIAVIVVVAMLDAVGVPPMIMVLFTGMCFVIWLF
                     ARRAQAREVERIFDFYIAADAVLREEERRWYGFEIAEVIEHGESLLEIMPDPPPLNYY
                     ALGALYQRLGNHAASVEYLSRVTEDERFDETHRIAPSPQLRRYVSMLRRIEYQPSLAP
                     QTLAAIRSLERARQRNAGKLLSESREFVEVAQHAVKEEKPVAEVTEAQKFATSLPLSA
                     ISAPPPITTVLHDVYQDETRTSN"
     gene            complement(15936..16991)
                     /locus_tag="VFH96_05020"
     CDS             complement(15936..16991)
                     /locus_tag="VFH96_05020"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET6973362.1"
                     /translation="MKRIAVIPGDGIGPEVVREAVRVLRHLQQTNGLELELVDFDWGA
                     EKFLRDGVTLPDGALEMLTAEFDAILAGAFGDPRVPSNKHAEDILLGMRRGLDLYINL
                     RPVRLLNDRLTPLLNRKTTDIDFVVFRENTEGAYVAAGGFLKHGTADEIALQDEVNTR
                     RGVERIIVAAFEYARANGRKRVTMADKSNVQRFGGDLWQRVFKEVAAGYPEIAANHQY
                     VDAMAMYMVLDPSQYDVIVSNNLFGDILTDLGAAIQGGLGLAASGNIHPGRVSLFEPV
                     HGSAPPLAGKGIANPIGSILTLAMMLEYLGFQTLSIEIEDAVRSAISANETTRDLGGQ
                     LSTEQAGAAISARLGKK"
     gene            complement(17001..17441)
                     /locus_tag="VFH96_05025"
     CDS             complement(17001..17441)
                     /locus_tag="VFH96_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6973363.1"
                     /translation="MLTSPEILVPIDFSPCSVNALRVAVGMAAPEGDLTLLHVVDEDF
                     IQAAVAAGVGSTEDLRGRLKEQAEASFANVLEGIEIGNVDIEKMIVIGLPFVEILKIA
                     RDLDLPMIVMGIRGQSTPAEEILFGSTAEKVLRATRVPVLCVPY"
     gene            complement(17500..18528)
                     /locus_tag="VFH96_05030"
     CDS             complement(17500..18528)
                     /locus_tag="VFH96_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6973364.1"
                     /translation="MLVAPLVFIACPKNDPPSRPRATVSSSTRPIDPAPPVAFNGDRA
                     MDHVKKQIDFGPRPPDTEQLAKTRAYITNELKSYGLTVSLDEFTAKTPQGEKKMANIV
                     AEVPGETKTLILITSHYDTKFYKDMSFVGANDPAASVGTLMEIGRVLGSRKEKPKMTY
                     RLVFFDGEESFCEGWSECGDEQSPDNTYGSRHYVSQLRAHNELENTRAMILLDMMGYK
                     NLELGRDPTSTRWLQDIIWQTGRSLGYDKVFVDRQEGVGGDDHEPFINAGVAAVDIIQ
                     LTSYPHWHRADDTIDKVSAQSMKTVGETILTSLPRITEHVTQKGTKEEKPQKAQTEPS
                     AVAPGLVP"
     gene            complement(18579..19091)
                     /locus_tag="VFH96_05035"
     CDS             complement(18579..19091)
                     /locus_tag="VFH96_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6973365.1"
                     /translation="MSVSITFEPSGINGLVAEGTYLIDAARRLGAPLGDGCTAGKGEC
                     PSCVVSVKAGANLLSPPSVAEEQQLGAEQLDQSLRLACQVKIESHGEVVVMAATRPQR
                     SEPVDLGSDLTKRFGTLPLSKKLATLVQLEAITMSEAFDSAIEKPLAFGSKTFDAILN
                     KAKARGAQKK"
     gene            complement(19156..19644)
                     /locus_tag="VFH96_05040"
     CDS             complement(19156..19644)
                     /locus_tag="VFH96_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET6973366.1"
                     /translation="MTTAEEPLTEEQEVFLKHIQRKGLKRTSQRDLILDVFLRTEEHL
                     SNEDLYQLVKKEDPTVGQTTVYRTLKLLTEAGLAREVRFGDGRAHYEHNYKHQHHDHM
                     ICSECGKIIEFFSAELEAIQDAMAAKHHFEITQHLLRIIGVCAECSAARRQSERTLTA
                     RS"
     gene            complement(19729..20709)
                     /locus_tag="VFH96_05045"
     CDS             complement(19729..20709)
                     /locus_tag="VFH96_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973367.1"
                     /translation="MPDHKHLESIEGSAKPADIFQPYLQGVELFEDMDAVFQEARQLA
                     AGEHPSADIPEVDHVKRGVAVVTPGRLIMLDPCPDRDSVRENELLPMLAVLPPKSPLN
                     ISVISHTRIEALHQDMTKAIPFRGYLLAWATAGHNVIVFEGHPSAFESGVRDADVLLM
                     DSGMLPFVQFNWVEAAFRVMQPDGKIFLHDRKKFGLSIISKPEGQPAPKPNLEANYVE
                     FLLRILMNAAQTSAEITAGEMVPDLLGFATKSTDRNWLNHNIERDKLNPEKVIDILVH
                     RAGWRWYNSFKTTRKLKFRVPLTNGTVRNYPFTLTLTKNVAGKRQLRIER"
     gene            complement(21110..21487)
                     /locus_tag="VFH96_05050"
     CDS             complement(21110..21487)
                     /locus_tag="VFH96_05050"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HET6973368.1"
                     /translation="MAGFIERERWKSFLDEFTKRNQFRATRLEVVGEIGAQEEEEYLP
                     LVGVSFEPKGSAAGSVGIVLGGESTKDERHIEHFIPKPERIAPLIGPEGFEGGLGFED
                     QDGGKTILTFAKLPEIPETTAGH"
     gene            complement(21506..22513)
                     /locus_tag="VFH96_05055"
     CDS             complement(21506..22513)
                     /locus_tag="VFH96_05055"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET6973369.1"
                     /translation="MSYADVVSKAAPAVVTIHSQMRVRQPQQYPFGNDPFFREFFGDR
                     IPQQAPEQRREGLGSGVIVGQDGYVLTNHHVIDGADRIKVDLNDNRTLEAKVVGSDPP
                     SDLAVLKIEASGLPVLALGDSDRTRVGDVVLAIGNPLGVGQTVTMGIISAKGRQTGLS
                     NGSFEDFLQTDAPINQGNSGGALVSTNNELVGINSQILSPSGGSIGLGFAIPSNMARR
                     VMDQLINTGKVRRGQLGITVLKIPSEEASKLGVDQGPGVVVYQVQPGSAADRAGLRRG
                     DIITALNGTAVNDPNSFRNIIAGTAPGSEVTLTVKRDGREQQVRATLDEFTPQAEQPR
                     R"
BASE COUNT         4885 a         6797 c         5963 g         5122 t
ORIGIN      
        1 ccgccctgat gggccacctg catcggggga cgcccgtatt gattcgtccc cgcaaacatc
       61 accttgccgt cattcaccag cgaatcgacg tgaaacacct cgcctgcaac aaaccgcgca
      121 agtacataat aagacgcacg ctcgcgcaga ttctgccgct cattcatctc gtgcatcgcg
      181 cgccgtactt cttccggctc actgaccttg cgaattccaa tcgcagaaac atcagaacgt
      241 ggcttgatca cccacggtcc cggcactcgc tgcatgaagt cgtcaacttc ttcgacattc
      301 acgagcggca cgaattcggg aacatcgatt cccgcacgcc ggctgtggac cgccatcgac
      361 agcttgtcgc gaaacacctt cgcacccgag ctgctcaaac ccggcaaaca caagtgctcg
      421 cgcatcaatg ccgctgtgac aacatcaaac tcttcgagcg caaccacccg atcgatcttc
      481 atcttccgac cgagaaacgc cagcaggtcg atgaacaacg gtggtccagc gtcattggga
      541 actgcgatca gatcgtcgag actctcccgc ggccagtctt cgtgaaggac cttctctttc
      601 gtgatcagaa tcacgtgaca gccgtgagct ttgcattcgc gaataaaatc agcgcctttg
      661 aaataggtgg cgaggcagat gatgttgagg gggtgattct cagtttcagg catttggatt
      721 ttctctcttt tcagaggttt gattatgctg ttatcctgta aatcctgcaa aatcctgtaa
      781 gaacagtgat gattattaaa ggaccgtatc ccgatatatc catacccgaa actgcgctta
      841 cgccgttcgt gctgcatcgc gccaaagagc tcggcgataa gcccgcgctg attgacgggc
      901 ccaccggacg cacgttcagt tatcaacaag tcgctgactc gatcagcatc gtggcccaca
      961 atcttgccgc gcgtggcttc aggaaaggtg acgtctgcgg catcttaagc ccaaactgtc
     1021 cggagtatgg tatcgcgttt catgcggtgg cgactttagg tggaatcgtc acaccaatca
     1081 acccgcttta cacacgttac gaagtcggac atcaactgaa agactcgggc gcgcgcttcc
     1141 tcatcactgt gcccgcctgc gcagaaaagg tgcttgaagc agacaataaa gccggcctcg
     1201 atgagttaat cgtcttcggc acactgccgg gcgcgacgcc gtttgacgag ctgctcgtcg
     1261 ataacggtcg cgccgaacaa gtcgagatca atccccgcga agatctgatc gctcttcctt
     1321 actcgagcgg aaccacgggt ctgccgaaag gcgtgatgct cacgcattac aatctcgtcg
     1381 caaacctccg gcagatggaa gggctgtgtt acttctacga cactgacaca ctaatcggcg
     1441 tgttgccact cttccacatc tacggtctgg tcgtggtttt gaacatggga ctctacagcg
     1501 gcgcgacagt cgtgatgatg ccgcgcttcg agctcgagtc ttttttgaaa acagcgcagg
     1561 actacgaagt gacgctggcc catctcgtgc cgccgatctt gctcgcgctc gccaagcacc
     1621 cggtagtaga caattacaaa ttaccgaaac tccacacgat cttttccggc gcggcgccgc
     1681 tcggcgaaga tctaacccgc gcgtgtatgg agcggctcgg atgtagcgtc cggcagggct
     1741 acggtatgac ggagacaagc ccggtcactc actcatcgcc tgcgccgccg ttgccggtca
     1801 agtttggttc tgtcggtgtt ccggcgccga ataccgagtg caaagttatc gatctcgaaa
     1861 cgggtgagca actgggttcc ggacaaaagg gtgaagtgtg tatgcgtgga ccgcagatca
     1921 tgaccggcta cctgaacaaa cccgaagcga ctgcgcagac gatcgatccc gaaggctggc
     1981 tgcacacggg cgacatcgga tactacgacg acgacgggca cttcttcatc gtcgatcgtg
     2041 cgaaggagtt gatcaagtac aaagggtttc aggtgccgcc ggctgagctt gaagcagtgt
     2101 tgttgacgca tccgtgtgtg gctgatgcgg cagtgattcc gtatccggat gaagaagcag
     2161 gcgaggtacc gaagggcatc atcgtcttgc gagaaccggc ggaggctgat gcaatcatgg
     2221 agtttgtggc cgagcgcgtc gcacctcaca agcgcatccg gcatcttgaa ttcgtcgaca
     2281 aaatcccgaa gtccccgtca ggaaagatcc tgcgccgcgt tctggtggag atggatcgta
     2341 caaaagtgtt ttagttcaat gtccgcgcaa tccgacaagc taaagcatgt cggacatcga
     2401 acgcttgaca gtctcccagc ctgcagaata gagtcgcccg taccaactct ccacgtggtc
     2461 acgcttaaaa agttgaggag gtaatgacat gcattctcgt cgtttacatt tggtgcttgc
     2521 cagtattctc ttagccgctt tcgcggtcgc ggctcacgcg caaacacccg ccgacgtctg
     2581 gcctgaatgg gaaacgttaa gtcctgaaaa cgaagaattc actgtcttga tgccgaagaa
     2641 cccgaccacg gaatcaacga catttccgta tcacaagatg gagatgaagg cacggctcta
     2701 catgtcgaca tcatctgccg gacctgtgct tgccatcgca tcgttgagcg gtatcaaatc
     2761 cgatccggcg caatacacgg aatttgcgcg cttcaattcg tacatcgacg ccttcaaaac
     2821 tttcttcccg gtaaaggttc gatctaaaga gacgctcgtg aagatggtgc tcgtgagcag
     2881 ccgtcctttt cacggtcaca ccggcaggtc atacaagatt acgcttggcg acttgaatgg
     2941 gtcagtcaat gccttcgtaa cgcgaaaacg tttctacgcg atcgtttcac tcaacaccaa
     3001 aaaggacgag gcgctggaag aaaagtttct cagctcattc gttctacccg agcgacagaa
     3061 cgagccgccg aagactgttg ccagcacgaa tccggaccag cagaacccaa atccggcgga
     3121 aaagcctctg gatcccaaca cccagaatac ggtccagtct gaaggcgacg ctacttcgcc
     3181 ggcgactcct aataatgcag aaaccccggc cgcacccacg actcccacga acactcgaca
     3241 aggtccggga ggagaggcag gaaagaaggg accgattaac ggcggcatgt tgaatggcaa
     3301 agcgatttac ttcccggtgc cggaagctcc ggcaggagac gcgaatggag tcgtgattgt
     3361 tgcgattctg gtcgatgagc agggatcggt agtcgacgcg cgcgccgtgt ctggtccaca
     3421 gcacctgcaa ccgacggctg tcgcggcggc acgtttggcc cgcttctcgc cgacattgtt
     3481 gatgggcgag cccgtcagag ttagtggtac gctgagttac aatttcgtca aatcaaatta
     3541 ggccacagat aaacacggat gacaccgatc agaaatgctg acctgcctct ttctgatccg
     3601 tgttatccgt gtttatccgt ggctaaatag atggagctgc aatcaaggat gtccactgga
     3661 atagctacct cgtgtcccgc gtggtcgata ccgggttacc tcgaagtgcg taacgaccac
     3721 ctgatcgtca acggtgtcga cacgctggaa ctcgtcaagg aattcaactc gccgctgttc
     3781 gtgttttccg aagctcgcat tcgcgacaac atcaagcgcc tgcaactcgc ggctgacgca
     3841 gtcgatcgcc cgatcaagtt ttgctacgcg tcaaaagcaa attcgaacat gtcgattctc
     3901 aaggtcgtcg ttgagtcggg gatcgatatc gaagtcaaca gcggcggcga gctattcaaa
     3961 gcactgcgca tcggtttcca cccccgtcag attatcttca acggcacgag caagtctgac
     4021 gacgaacttg acgatgccgt acgcgcaggt atctacgcaa tcaatgtcga ttcgatttac
     4081 gagatcgatc tggtggaaca agctgtcgag cgtctgcgcg cgcatggtga aaacgtcgcg
     4141 cccgcgcgcg tcacgttgcg cctcgtaccc gagatcggca cgcgctcaca tctcggtctg
     4201 caaaccgcgc tgctgacgtc gaagtttggt atctcgtcgt ccgaagttct ggcagcgttt
     4261 aggcgtggtc tggagaaacc ggatctggtt catgtttgcg gaattcacat tcacgtcggc
     4321 tcgcagacgc cagacgttga accatttgcc gaagcgttca aaagcatgtg ggaacacctc
     4381 gtcaccattc atcgcgagac gggccacacg ctcgaacaca tcaatctcgg cggcggcatt
     4441 cccgtcaact acctccgcga tcgctcgcag gcagatcaac ttccagagca tgagcgcgac
     4501 atgctcggcg ccgagctcga gccttccgca gtgctgaagg aagcgctgcg cgtcgcgcgt
     4561 gaatctgctc gcgccgcaga cgccgagcac ctgctcgatc aagtgacgat cttgctggag
     4621 ccgggtcgca gcatcatcgc tgacgcaggc ctggtgctaa cgactgtgcg taatatcaaa
     4681 agccgtcccg aaaccggcga cgtttggctg ttgaccgatg ccggttacaa catcatgctc
     4741 tcgatgaata attacaagtg gtactaccac ctgatctccg cgtcgcgagc aggcgcgccg
     4801 tacgcgcatg attacaaagt tgccggtcca ctctgcgatt cgggcgacgt ttatttcgat
     4861 atcgagcgcg aagggcgttt gccggattac cgcaagcttc ctgaagatgt tggaccaggc
     4921 gaagtgctcg ccttgctcaa ctgcggcgct tattcattgg cccagatgtt ccactacaat
     4981 ggccggccac tgcccgcggc ggttttgatc cgtggggata aatcagtttc tttgatccga
     5041 agaagagata gctacgacga tctgatagcc aacgacattt tttaaccgcg gatggcgcgg
     5101 atttttaacg gatgcaaact caacatacgc aactcatccg cgggctggga ttgatcgctg
     5161 caatctcagt caacgtcgcc aacatcatcg gcaccggcgt ttttctcaaa gggcgggtga
     5221 tgacgtgtaa tgtcggcacg cccggcaaag cgctcatagt ttgggtgata gcgggcgtac
     5281 tctcgctcgc tggcgcgctc acctacgcgg agctgctcgc gatgatgccg cgcgccgcag
     5341 gtgagtatgg aatcatccgc gatgcgtatg gccggccgct gagttttatc tatggctggg
     5401 cccagtttct gatcgcgcgt accgcttcgg ctgcggcgct cgcagtcggc ttcgcgatct
     5461 tcctcaacga tcttgttaaa ggcgcgctcg acaccgtcta ctttaaatac acatttcccg
     5521 gcgggcacgt cgtttcgttt ggtcgactgc agcttgttgc acttaccgcc atcattgtca
     5581 cgacgttgat taactgtgcg gcagtgcgcg tgagtggcgg tgtggctagt gtgctcacgg
     5641 gcatgaagct cgtgctgctg ctcggtatcg gctttggagc tttcttttac agcggcggcg
     5701 attggagcca tctcggcatg gccaacatcg gcggcgcttg tgaaggcgta gcgatcacca
     5761 cgggcggcat ggccgggttt gcagccgcga tgctcggtgc gctctgggct tacgacggct
     5821 ggaacaacat cacgtttctg gcaggcgaag tgaagaatcc tggtcgaaac ctgccgctgg
     5881 ctttgatcgg cggcgggctg gtggtgatgg gactctatct cttcgttaat gtgagctact
     5941 tccacgtgct ctcaccgaca gagatggcga gcgtgccggc gtcttcgtcg gtagcggcgg
     6001 aggtagttaa gagattgctt ggcgctggtg cagtgacact catggcggcg gcgatgatga
     6061 cctcatcgtt cggcgcgctg cacgcttcgc tactcgcaac cgctcgcata ccttacgcgc
     6121 tcgcaaaaga cggcttgatc atcaagagcc tcgcgcacgt ctcgccgcac acacatgtgc
     6181 cggtgcgtgc cctcatggtc cagatgttct gggcgtgcat cgtggcgctc tcagggtctt
     6241 acgacacgat tactgactac gcaatcttcg cgttgacgtt gttctacata ctcgtcgccg
     6301 catccatctt cctgttccgt agacgtatgc cggacgccga gcgaccttat cgcacgtggg
     6361 gttatccggt cgtgccggtc gtgtttatct gcgtctcggt ctggctcatc ttccaaacca
     6421 tccgcaacac gccacagcag tcagcgatcg ggctcggact gatcatgttg ggccttcctg
     6481 tttactggct aataagccga tcacgaaaaa gcacaaaaga aatctagcca caaaaggcac
     6541 aaaaagcaca aaagtaaatg aatctgtttt gtgccttttg tgccttttgt ggctaatttc
     6601 ttaaaacttg aatgagtggg gattgcttgc cttcactggt ggcgaggaaa gctttgccgt
     6661 cgaggcggta ggtgatcgat tcaccctgtt tgcgcttgcc cagatcgaag ctgatcattt
     6721 tctgtttcca gatatcgtcg aagtttgaag cacctgccga caataccaac tcataacccg
     6781 aaaaataatc acatagcgcg acgcgtcttc catcaggaga gatgctgccg ccggtgatcg
     6841 cgccgccgaa tagactcggc acacgaggct cgcccactcg tcgcatcgtg atcgattggc
     6901 ctgatgtaaa tggcgcgata gcttcataaa ccactggatt cgcgatcaag actttcgtga
     6961 cgatgtatag gttcccggtg cgtggatgga ccagcagggc ttctgcgtca tgcttgccat
     7021 cgggatattt cagcctgatc gcctcggcag gctcggtaga tccgggccgg ctcttcgtaa
     7081 acttgcgcgt agaagttgat agtgccggtt cagcgacgcg atagacgatc acttccggac
     7141 gcacggcatc gttatcacct atatcgccga cgtacagata cgatctgttt ggttgtggtc
     7201 cggggccggc ggcgatgtct tcccagtcgc gtgcctctgc gccgctcacg cgaaacgtgc
     7261 cgaaggaatc accgcgcgta tcgaaggcgt agatgaatgg accatcgccg gaatcattat
     7321 gtgtccaata cgcgcccggc atcgtacgcg acgcgacgag accactgctt tcggagattg
     7381 atttatcttt gatggtggcg agcgttgccg gctgaccgta agtgaccaac cagacaatta
     7441 ataagtaaat gtgcattaga caggatttac aagatttttc agaatgaaca agatgaaatc
     7501 atgtaaatct tgaaaacatc ttgtaaatcc tgtctcaact acgctgcaac agtgcggcgc
     7561 gggcgacttc tttgtcgtcg aaatgaaacg tctcacggcc gataatctgg taatcctcgt
     7621 ggcccttccc ggcaatcaac acgaggtcgc cggcctgcgc ctcggcaatc gcctggtgga
     7681 tcgcctcggt ccgatcggcg atcttgcgat aaggtttgcc cgttcgctga attccctctt
     7741 ccgcgtcgca aagtatctgc tccggatctt ccgtccgtgg attgtccgac gtgagaatca
     7801 caacgtcgct caagctgccc gcagcctcac ccatcggcgc tcgtttcgtc cggtcgcgat
     7861 cgccgccaca cccgaacacc gtaatgatct ttccctgcac cacctcgcgg gccgtgcgca
     7921 aaacgttcaa gagcgcatca tcactatgcg cgtaatccac cacgactgag aaaccgccat
     7981 catggaccac gcgctcgaaa cgtcccggcg cgcccgtaca cttctcgagc gccgctgtca
     8041 cgacctcgag gctgtaaccg agcgcgagtc cactcgcgac cgccgcgagc gtgttgtaga
     8101 tatgcggtgg gccaacgagc ggcgaatgaa aatcgcgttc gcccgcgggc gtcttgaggc
     8161 gaaaccgcat accactcaac gaaaactccg gatcgcgcgc agtcacatcc gcatcgctat
     8221 tcaccgcgta acgaatcaca cgcatcccat ctttctcgag tgatgcggca agctcaacac
     8281 cgtaaggatc gtcgacgtta atgacggagt ggcgcggtcg cgttccgagt cgaccatcaa
     8341 acagtcgctg cttcgcgtac cagtagttct ccatcgtctt gtgatagtcg aggtgatcgc
     8401 gcgtgagatt tgtaaacacg gcaaccgcgt attcgagctc gtcacaacga tgaaaatcca
     8461 tcgcctgcga agaacactcc atcactgccg tgcgacaacc aatctccacc gcgcgccgca
     8521 gcagacgctg catgtcgggc gcttccggcg tggtgcgatc cgctttgaag cgctccttgc
     8581 cgagacggta ttcgacagtt cccgtcatcg ccaccaactc acccgcttct tcaggaatcg
     8641 acgcgatgag atatgcagtt gttgtcttgc cgttcgttcc ggtgataccg acgagttgca
     8701 gctcgcgtga cgggtggttt tggaccgcgg cagccgcgag cgccatcgca cggcgaatgt
     8761 cttcaacttg cagccacgcg ccgttgaaac cctcgggcgc cggacgttcc gacaacacgc
     8821 ccgccgctcc ctgctccatc acttgcggca caaacttgtg cgcgtcgaac aacgctcctg
     8881 caaccgccgc gaacaacgtc cccgcaccaa cacgccgcga atcatgactt acatccgtga
     8941 cgatccgcgt tccctcaccg ctgagtttcg cgtccgttaa ttctgctatt cgttgtgtat
     9001 caatcacgga ttgttactct gcggcttcac cacgaccgcc ggaatattct gatccttcac
     9061 taactgattg aacgccggca catcggttcg catcaactgc ccgagcttct gcaactgcgc
     9121 atcaatctgc gcgactactt catcatacac cgcatacgat tgcgcagtcg gcgacgcttc
     9181 ggcactcgca actacaccac cgagcgccgc gagcttgtta ttcaaacgga tcgggaaatt
     9241 caaaggatcc tggctgctct ggttcttcgt ctggtaaagc gcctcttcga tcgcagtcag
     9301 gttcttattc agcgccgtgc ccgcatcgtt gacaactttg aagttcggct gaccaccaac
     9361 gcgcttcagc aagtcttcca cctgcttgcg cacgtctcgg atctgaatga tcgcgttgtg
     9421 tgtttcgctc aacttgtcgc gtatcttcaa attcaactcg agctgtttgg cgtaatccgc
     9481 cggcgtggtc gacagacgtg gatcggcttt gatctcgaac gcctgcgtga agctcttacc
     9541 ttcgacagtg agcttcacct ggtaagttcc cggcagcagt tttggaccac gtgtctcacc
     9601 cgcccaaaga atcatccccg gaaaacgcac cgcatcggga tagcgcgtgt cccagacgaa
     9661 acgattgagc ccgacatcgg tcgacacagg cggcgcgcca ccaccaccga aaaatccacc
     9721 ttcacccgga ggcgcagtgg gcggcccagc cgcagcagca ccctcgccct tcgctttcgc
     9781 tgtaaacttc ctgatcgact tccctgccga atctaaaaac tccagatcga catcagcagt
     9841 tggtttcgct ttcaacgaat agtaaaccac gacgccgttc gcaggattcc tgccgagcgt
     9901 cgccgttggt ccaagcggaa aaccaccgcc cggcatgcga taactctcct tcggtttgta
     9961 caaacgcgtc tcgcccgccg cactgaaccc acccgtgtcc atcatctcat gcaacaccgg
    10021 caggtcgtcg aggatccaga acgctcgtcc ctgcgtcgca accaccagct ccttctcacg
    10081 tttctgaatc gcgagatccg tgatcggcac gatcggcaga ttcaactgca tcgactgcca
    10141 gtgctcgccg ttatcaaacg acacgtacat ccccgtttcg gtgcccgcgt aaagcagccc
    10201 gcgcttgttc ggatcttcgc gaatcacgcg tgtaaacgta gtatcgggaa ttccagtcgt
    10261 gatcttcttc cacgttttgc cgtagtcgct cgtacgatag agatagggct tgttgtcgtc
    10321 gaacttgtac atcgtcgccg caacgtacgc ggtgcccgga tcgaagggcg aggcgtcgat
    10381 cgagttgatc tggatccact ccggcatgat gtctttcggt ggcgtgacat tggaccagtt
    10441 cttgccgccg tcgcgtgaca cgtgcacgag cccatcatcg ctgccggccc aaatcgttcc
    10501 ggcctgcacg ggcgactcca tcaccgtgaa gatcgtcccg taatactcga tgctcgtgtt
    10561 gtctttcgta atcggtccac cggacgaagc ttgttttgtt ttgtcgttgc gcgtgagatc
    10621 cggactgatg atttgccaac tgttgccctc atccatcgac atgaacaaca cgttcgcggc
    10681 gacataaagc ttattcggat cgtgtggtga aaacacgagc gggaaattcc attgaaagcg
    10741 atacttcaaa acatccgcgc ccgatcccat cggattgttc gggtaaggat tgacgtcgcg
    10801 tagctggccg gtgcgtttat cgtagcgcgt cgtcaaacca ccgtaagatc cggcgaacac
    10861 gatgttggag tctttcggcg atggtgcgat ccagcccgac tcaccgccac caacgtcata
    10921 ccaatcgctc gtgccgatgc cgccttcagt cgtgcggctc gcgatgcgca cagtcgagtt
    10981 gtcctgctgc gcgccgtaga cgttgtaagg aaagtcgttg tcgagcgcga cgcgataaaa
    11041 ctgcgcggtc ggttggtcct gttcggtcca gctccggccg ccgttatacg aaacattcga
    11101 gccaccgtcg ttgctctcga tcatgcggtt cggatcgtcc ggcgcgatcc acagatcgtg
    11161 attgtcgccg tgcggcacgc cgatgcccgt gtaagtgcgg ccgccatcgt tcgagcgata
    11221 gaagccggta ttcaaaatgt aaactgtatc ggcgttcttc ggatctgcgt aaatgcgcgt
    11281 gtagtaccag gcgcgctggc gcaggcgacg ctcttcgttg actttggacc aggtgctgcc
    11341 gccgttgtcc gaacgaaaca cgccgccatc ttcagcttcg acgatggccc agatgcgatc
    11401 gggatttgca ggcgagactg cgatgccgat tttaccgacc gtccctttcg gcagaccggg
    11461 atttttcgtc aactcattcc aagtgtcacc gccgtcagtc gatttaaaga tcccaccgcc
    11521 tggaccacca ctctcgagtg tccagggttt gcgagagact tcccagaagc ctgcataaat
    11581 gatgtttgga tttgtcggat cgaggatgag atcgatcgcg cccgccttac tgccacggct
    11641 cagaatcttc tcccaggtct tgccgccgtc tttcgaacga aacacgccgc gctgctcgtt
    11701 tggtccccag acgtggccga gagccgcgac gtaaacgaga tcgggattct tcggatgcac
    11761 cctgatgcgc ggaatctgtc gtgtgtcttc aagaccgacg cgcttccacg tcttgcccgc
    11821 atcgttcgac ttgtaaacac catcgccgtg cgagacgttg ccgcgaatcg gcgactcgcc
    11881 catgccgaca tataccgtgt ttggatcaga atcagcgact gcaatcgcgc cgactgagcc
    11941 cgtgccgaag accgaaccat cgcttactga ttcccagttg atgccgccgt cagtcgtctt
    12001 ccaaacgccg ccaccagtcg cgccgtagta aaaaacgaac ggctgcgagg gaacaccagt
    12061 gactgccgcc gagcgtccac cgcgatatgg accaatgctg cgatattgca acgccttcaa
    12121 gagatcaggc ggttttggcg gtgcgccgtt ttgcgcgtga actgcggaaa gagtcagcgc
    12181 gagggcaaga cagagagaga gggacttcaa cattagtggg ctccttttcg aatatttagc
    12241 cacagattac acggatgaca cagatcaatc cgtgtttatc cgtgtgaatc cgtggcttaa
    12301 tttctttctt ctcgtaatgg gcggctcatt agttctgaaa cggcatcgcg cgccggcttg
    12361 ccgtcataaa gcactgcgtt cacttcattc gtgatcggca tctcgaggct cgcgcggtcc
    12421 gcgagtttct tgacggcgcg cgcggtgttg atgccttcgg cgacttcgct cagctcggca
    12481 gtgatctcag cgagcgttct gcctttgccc agctctgcgc cgacaaagcg gttccgcgaa
    12541 agattaccag tacacgtcaa gaccagatcg ccgaggcccg caaggcccat caacgtttcc
    12601 atctgcgcac cttcgcgtcg ggccagacgt gtgatctcgg cgagaccgcg cgtgatgagg
    12661 gcggcgacac tgttcgcgcc gaagcccaga ccgtcggcca ttcccgcagc gatcgccatc
    12721 acgttcttca ccgagccgcc gatttcagtt ccgacaacgt cggcgttcgt gtaaatccgc
    12781 agattctcaa aactcagatc gttctgaacc gcgcgcgctg ctgcaatgtc tttgctcgcg
    12841 gcgacgatcg cggtcgggtg gccggccacg acttctttgg cgaacgatgg accagacaat
    12901 gtcacaaacg gatgcgttcc ggcgacgact tctttggcga tctccgaaat tcgtttaccg
    12961 ctctcgatct cgatgccttt ggcgacgttg acgatgattg ccgcttcatg cagatgcgat
    13021 gtcaacgcca acaatgtctc tcgcgctgcg tgcgatggca cggccagcag caccatcgac
    13081 gcatcttgca gcgccgcttc aacgtcaccg gtcgcagtga cgttatcagg aatcgatgtg
    13141 gcgctgagat agcgtgagtt aactcgctgc tcgttgatcg aactcacgac cgcatcgttt
    13201 cgcgaccaca atcgcacgct atgtccggcg cgtgcagcaa tcacagccag tgctgtgccc
    13261 caactccccg ctccgatgac tgcgatgtgc ttcatccttc agatccgcga ctccgttccc
    13321 gacagcaaac gttgaatgtt gccgcgatgc gcaaacacga tcagcgccgc tcccacaatc
    13381 gccgctacca acaaaggtcg cgagtcagac tgcacccaca caagcacggg aatcaaaata
    13441 gctgcagtga tggaacccaa cgatacgtat cgtgtcagca gcacgagggc aacgaagacc
    13501 acacccgcgc acaacaacgc gatcggagct agcacgagaa agatcccaac gcccgtcgcc
    13561 acacccttgc caccacgaaa acctagccac accgggaaaa tgtgtccgac gagcgcagca
    13621 atagcagcaa ccgcgatcac ccaatcatca ccaccaaaag ccttcgcgat cagcaccgcc
    13681 acacaaccct tcgcagcatc aagcaacagc gtcaacacac ccgccgcttt gcccgcccgt
    13741 cgcgaaacgt tcgtcgcccc ggtcccaccc gatcccgtct ctcttacatc cgcgccgatc
    13801 ttccctcgca caatcaagta accaaacgga atcgatccga gcaagtacgc ggcgacaacc
    13861 aaaagaatct tgtccatcgt gtaaatcatg taaatcctgt gacaagattt gccatgattt
    13921 acaaaattga caagattaat tagagcaatc tgcgtcgcaa catatccaac gcggcttgcg
    13981 aggcacgcca gcgaatcaga tttcgatcgc cgggcaggtt gagccggcga tgctctgtct
    14041 tcacatcatc cgcaagcgcg atgaaaaccg tcccgacggg cttatcttcc gtacctccac
    14101 ctggtcccgc aatccccgtg atcgacaacc cgaaatctgt tccaatgcgt ttgcgaacgc
    14161 cttcagccat cgcctcggca acgggcgcac tcaccgcgcc atgctccatc aacaacattg
    14221 gctcgacgtc taactccttc gtcttcactt cattcgcata cgcgatcaca ccgccaataa
    14281 aatatttcga agacccgggc acgtccgtga tccgctgcgc cagcaagccg ccggtacaac
    14341 tctccgccac tgccagcgtg taaccaccca gcgaaagttt cagcccaaca acttcctcca
    14401 tcgtctcacc cgcaaacgag aagacggcat tcccgagtcg ctcctcgatc tgcgctgaca
    14461 aacgatcgag taactctgaa gcctccttct cagtgcgacc gcgcgcagtc agatggatct
    14521 cgatctcact gtgattgaac agaatcgttg ttaccggatt gtcatactgc gtataaaccg
    14581 gcgcgatctt ctcgtcaacc gccgactcac ccatgcccgc tacgcgcaac atccgtcgca
    14641 cgacacgcaa acttcccgcg cgaccggcaa gtctctcggc aacgtgattt tcaaacatcg
    14701 gacgcatctc gcgcggtggt ccgggcagta gaacaatcgc ggtgccctga tgatcgatga
    14761 acattcccgg cgcagtgccg tttggattcg gcagcacttc agcgtcttcg atcaccatcg
    14821 cctggcgcga atttcgctcc ggcatcgtca cgccaaaact ctgaaaccgc tgccggatct
    14881 cagccagcac gacttcgtcg agcaacagtc gtttgcgaag cgcgcgcgca gtgatcttgc
    14941 gggtgatgtc gtcttcggtc ggaccaagac cgccggtggt gatcaccact ttcgagcggc
    15001 gcacagcgtc tccgattgct tcttcgaggc gggcatcgtc gtcgccgacg atggtcttga
    15061 gtttgacttc aataccgagg cggttgagtt gttcggtgag ccagagacta ttggtatcag
    15121 tgcgatcggg cgcaagtagc tccgatccga tcgcaatgat ttccgctgaa agggattcga
    15181 acaagtcgcg ttagttactg gtcctggttt cgtcctgata gacgtcgtgc aacacggtcg
    15241 tgattggcgg cggcgcagag atcgcactca acggcaaaga cgtcgcaaat ttctgtgctt
    15301 cagttacctc ggctacaggc ttctcttcct tcaccgcgtg ttgcgctacc tccacgaatt
    15361 cgcggctctc ggaaagtagc ttgcctgcgt tgcgttgtcg cgcgcgctcc aaactgcgaa
    15421 tggcggcgag cgtttgcggc gccagcgatg gctgatactc gattcgtcgc agcatcgaaa
    15481 cataacggcg cagttgtggt gacggcgcaa tgcgatgagt ttcgtcgaat cgctcatctt
    15541 cagtcactct tgaaagatat tcgacactgg cggcgtgatt accgagccgt tgataaagtg
    15601 caccgagcgc gtagtaattc aacggcggcg gatccggcat gatctcgagc agactctcgc
    15661 catgttcgat cacttccgcg atctcaaagc cgtaccagcg tcgctcttct tcacgaagca
    15721 ccgcgtcggc cgcgatatag aaatcaaaga tgcgttctac ttcacgcgcc tgcgcgcgac
    15781 gagcaaagag ccagatcacg aagcacatcc cggtgaacag caccatgatc atgggtggga
    15841 ctcccaccgc atccagcatc gccacaacca caataaccgc gatcaccttc gtcgtgagtt
    15901 tccctgcgcc cgtcatgact ttactcctcg ttcttttact tcttcccgag acgcgcgctg
    15961 atcgccgcgc ctgcctgttc ggtagagagt tgtccgccaa gatctctggt cgtttcgttt
    16021 gcagagatcg cgctgcgaac agcgtcctcg atttcgatac tcaacgtttg gaagcccagg
    16081 tattctaaca tcatcgcgag ggtcagaata gatccaatcg gattcgcgat tcccttgccc
    16141 gcgagtggcg gcgcgctgcc gtgcaccggc tcgaacaacg agactcttcc gggatgaata
    16201 tttcccgaag cggcaaggcc gagcccaccc tgaatcgccg cgcccaggtc agtcagaatg
    16261 tcgccgaata gattgttact gacgatcacg tcgtactgcg aaggatcgag caccatatac
    16321 atcgccatcg catcgacgta ctggtgattc gcagcaatct ccggataacc cgcagcgact
    16381 tccttgaaaa cacgctgcca caggtcgccg ccaaaacgtt gcacgttcga cttgtcagcc
    16441 atcgtcactc gcttgcgacc gttggcacgc gcgtactcga aggcagccac gatgatgcgc
    16501 tcgactccgc gacgcgtgtt gacttcatcc tgcaacgcga tctcatcggc cgttccatgc
    16561 ttcaggaaac caccggcagc gacgtacgcg ccttcagtgt tctcgcgaaa gacgacgaaa
    16621 tcgatatcgg tagttttgcg gttgagcaat ggcgtgagtc gatcgttgag caggcgcacg
    16681 ggccggaggt tgatgtaaag atcgagaccg cgacgcatgc cgagcagaat gtcttcggcg
    16741 tgtttgtttg acggtacgcg tggatcgccg aacgcgccgg cgagtatcgc gtcaaactcc
    16801 gcggtcaaca tctcgagcgc accatcgggc agcgtcacac cgtcgcgcaa aaacttttcc
    16861 gcgccccaat cgaagtcgac cagctcgagc tcgagaccgt tagtttgttg caagtgacgc
    16921 agcacgcgaa cggcttcgcg cacgacctca ggaccaattc catcaccagg aatgactgcg
    16981 attcttttca tggtcagcga ttaatagggc acgcacaaca ccggcacgcg cgtggcgcgc
    17041 agcactttct ccgccgtcga accgaacagt atttcttcag cgggcgttga ctggcctctg
    17101 attcccatca cgatcatcgg caagtcgaga tcacgagcaa tcttcagaat ctcaacaaac
    17161 ggaagtccga tcactatcat tttttcaata tcgacgttac cgatttcgat tccttcgagg
    17221 acattcgcaa aactcgcctc cgcttgttcc ttgagacgtc cgcggagatc ctcagtcgat
    17281 cccactcctg ccgccaccgc ggcttgtatg aaatcctcat cgacgacatg aagcaacgtc
    17341 agatcgcctt cgggcgccgc catgccaacc gcaactcgga gcgcgttcac cgagcaaggt
    17401 gaaaaatcta tcggcacgag aatctcgggt gatgtaagca tcgctcctcc tacagaaccg
    17461 agcgcggtag cgccgggtca atgtcatcct caggcgtcct tatggcacta aacccggcgc
    17521 taccgcgctc ggttctgttt gtgccttttg tggcttttct tcttttgtgc ctttttgtgt
    17581 cacgtgttcc gtgatcctcg gcaaactcgt caggatcgtc tcgcccacag ttttcatgct
    17641 ctgcgctgac actttgtcga tcgtatcgtc ggcgcgatgc cagtgtggat acgacgtcag
    17701 ttgaatgata tcgaccgcag ccacaccggc attaataaac ggctcgtgat cgtcaccacc
    17761 aacgccttct tgccgatcga caaacacctt gtcgtatccg agcgagcgtc cggtttgcca
    17821 gatgatgtcc tgtaaccacc tcgtgctcgt cgggtcgcgg cccaactcga gattcttgta
    17881 acccatcata tcgagcagga tcatcgcgcg cgtattttcc agctcgttgt gcgcgcgcag
    17941 ttgcgaaacg tagtggcggc tgccgtaggt attatcagga ctctgctcgt caccgcattc
    18001 agaccagcct tcacaaaacg actcttcgcc gtcgaagaac accagtcgat acgtcatctt
    18061 cggtttttct ttgcggctgc cgagcacgcg accgatctcc attagtgtcc cgactgacgc
    18121 tgccggatcg tttgcgccga cgaagctcat gtctttgtaa aacttcgtat cgtagtgact
    18181 cgtgatcagg atcagtgttt tagtttcacc cggaacctcg gcgacgatgt ttgccatctt
    18241 cttttcacct tgcggagttt tggcagtgaa ctcgtcgagc gagactgtca gtccataaga
    18301 cttcaattcg ttcgtgatgt aagcacgcgt ctttgcgagt tgttcagtgt caggaggacg
    18361 tggtccgaaa tcgatctgct tcttcacgtg gtccatcgcg cgatcgccat tgaatgcaac
    18421 cggcggcgct ggatcgattg gacgagtact actcgagaca gtggcgcgcg gtctcgacgg
    18481 cggatcattt ttgggacagg cgataaaaac caaaggcgcg accagcacca gggtcaatct
    18541 tgtaaatcct gtaatcttgt ctaacaaatc ttgtccacct acttcttctg cgcgcctctc
    18601 gctttcgcct tattaaggat cgcatcaaac gtcttcgatc caaacgcgag cggcttctcg
    18661 atcgccgagt caaacgcttc tgacatcgtg atcgcttcca actgcaccaa cgtcgcaagc
    18721 tttttgctga gtggcagcgt gccgaatctc ttcgtcagat ctgatcccaa atcgacaggc
    18781 tccgatctct gtggtcgtgt cgcagccatc accactacct cgccgtggct ctcgatctta
    18841 acctgacacg cgagccgcaa cgactggtcc aactgttccg caccgagctg ctgctcttcg
    18901 gccacgctcg gtggcgacaa taaattcgcc cccgctttaa cggaaacaac acacgacggg
    18961 cactcgccct tccccgccgt gcagccgtcg ccgagtggcg cacccaaacg tcgagcagcg
    19021 tcaatgagat aagttccctc ggcaacgaga ccgttgatgc cggaaggttc aaacgtaatc
    19081 gagacactca tgcccaacct ccaaccgctg tgtacttgtt tggctagtgt aaatccgggg
    19141 gagacttctg caaaactacg agcgcgcggt gagggtccgt tcgctttgcc ggcgcgccgc
    19201 cgagcactcg gcgcagacgc cgatgatgcg caacaggtgc tgcgtgattt cgaagtgatg
    19261 cttcgcggcc atcgcgtcct gaatcgcttc gagctcggcc gaaaagaatt cgataatctt
    19321 gccgcactcc gaacagatca tgtgatcgtg atgttgatgt ttgtagttgt gttcgtagtg
    19381 tgcgcgcccg tcgccgaagc gtacttcgcg cgcgagcccc gcttcggtga gcaacttgag
    19441 cgtgcgatag acggtcgtct gtccgacggt cgggtcttct tttttaacga gctggtagag
    19501 atcttcgtta gagaggtgct cttcagtgcg caaaaaaaca tccagaatca ggtcgcgctg
    19561 agacgtgcgc ttcagaccct ttcgctggat gtgtttaagg aagacttcct gctcttcggt
    19621 taatggctct tctgcggtcg tcatcactag ggggagattc taacacctgg acgggtcgtc
    19681 ggaaagagtt cggctgtatg gtgggtttaa ttatctcact aaacttatct atctctcgat
    19741 gcgcagttgt cgtttcccgg caacgttttt cgtaagcgtt aaggtgaatg ggtaattgcg
    19801 aacggtaccg ttggtcaaag gcacgcggaa tttcaacttc ctggttgttt tgaaagaatt
    19861 gtaccagcgc catccggctc tatgaacgag tatgtcgatc actttctcgg ggtttaactt
    19921 gtcacgttcg atattatggt tgagccagtt gcggtcagtt gatttggtcg caaaacccaa
    19981 tagatcggga accatctcgc ccgctgtaat ctcggccgaa gtctgcgcgg cattcatcag
    20041 aattctcaag aggaactcaa catagttcgc ttcaaggttc ggtttcggcg ctggctgacc
    20101 ctcaggcttg ctaatgatcg acagcccaaa cttcttgcgg tcatgaagaa atatctttcc
    20161 atccggctgc atcacgcgaa aggcggcttc gacccagttg aactgcacaa agggcagcat
    20221 gcctgagtcc atgagtaaca catccgcatc gcgaacaccg ctctcgaacg ccgatggatg
    20281 cccttcgaag acgatcacgt tatggccagc ggttgcccaa gcgagcaagt agccacggaa
    20341 aggaatcgct ttggtcatat cctgatggag cgcttcgata cgggtgtgtg agattaccga
    20401 aatgtttagt ggagattttg gcggcagaac tgctagcatc gggaggagtt cattctctct
    20461 aacgctatct cgatcaggac atggatcaag cataatcagc cggcccggtg tcactacagc
    20521 cacgcctcgc ttaacgtggt ccacctctgg aatgtcggcg ctcgggtgtt cgccggcagc
    20581 caactggcgg gcctcctgga ataccgcgtc catgtcttca aacaactcga cgccctgcag
    20641 ataaggttga aagatgtcgg ccggcttcgc actcccttca attgactcta ggtgtttatg
    20701 atccggcatg atgttacaaa tatagcattc ccgatctcgc gggccacgcg aagccttgat
    20761 agatgcctta gcggaaagac gaaaggcttg ctagaatcac gcccacggac aacatgccgg
    20821 ggtggcggaa tggcagacgc tgcggactta aaatcctcca tccggcttct tacctagtgc
    20881 gacgaaacga tactaagcag tatttaccga cgtttcagcc gttgaaagta gcttacgtcc
    20941 tgcttcatat cattaggtga tccacgcatc ttccacgcat ctttggacca aagaaacgat
    21001 aagcccggaa atgtgagcag ttatgtcacg tagtcgaacg tctagcccta tatgcagggg
    21061 cctttgatgc ttttgccgtt agtggcccag gccgcgcaac actccggtgc tagtggcccg
    21121 ctgtagtctc cgggatctct ggtagcttcg cgaaagtcag aatcgtctta ccaccatctt
    21181 gatcctcaaa gcccagcccc ccttcaaaac cttcaggccc aatcagaggt gcgatccgtt
    21241 caggcttggg gataaaatgc tctatatgtc tctcgtcttt cgttgattcg ccacctaaaa
    21301 caattccgac actcccagca gcgctccctt tgggctcaaa gctcactcca acaagcggta
    21361 agtattcctc ctcttcctgt gcgccaatct ctccgaccac ttccaatcgt gtggcgcgaa
    21421 actgattcct tttcgtgaac tcgtcaagaa aactcttcca tctctctcgc tcaataaaac
    21481 ccgccatttt ctcctcctct catgctcatc tgcgtggctg ttcggcctgc ggggtgaatt
    21541 cgtcgagggt cgcacgcacc tgttgttctc ttccgtcgcg cttgacggtg agcgttacct
    21601 ccgaccctgg agcggtgccg gcgattatgt ttcgaaacga gttggggtcg ttaacagccg
    21661 ttccattgag cgcagtgata atgtcccccc tgcgaagccc agcccgatca gcggcacttc
    21721 caggttgaac ctgataaacc accactccag ggccctgatc gacacccaac ttcgacgctt
    21781 cttccgaagg gatcttgagc acggtgatcc caagttgccc acgccttact ttgcctgtat
    21841 tgatcaattg atccatcacc ctgcgggcca tgtttgaggg gatcgcaaat cccaacccga
    21901 tgctgcctcc cgacggtgaa aggatttgcg agttgattcc gaccagctcg ttgttggtac
    21961 tgactaaggc cccgcctgaa ttaccttgat tgatcggtgc gtcggtttgc agaaaatctt
    22021 caaagctgcc attgctcagc cctgtctgcc gacccttcgc gctgatgatc cccatggtaa
    22081 cggtctgacc tacacccaga gggtttccta tggcaagcac tacatcgccg actcgagtgc
    22141 gatctgaatc gcccagcgcc aacactggca ggccactagc ctcgattttc agtactgcca
    22201 gatcgcttgg gggatcggag ccgacaactt tagcctcgag tgtgcgattg tcattcaaat
    22261 ccaccttgat tcgatcggcc ccatcaatga catgatggtt tgtcaaaaca taaccgtcct
    22321 gaccgacgat cacgcccgag cctaatcctt ctcgccgctg ttcaggcgct tgctgtggaa
    22381 tacgatcacc gaagaactcc cgaaagaacg gatcgttccc aaatggatat tgctgcggct
    22441 gtcgaacgcg catttgcgaa tgaatcgtta ccaccgccgg cgccgctttg ctcaccacgt
    22501 cggcgtagga catcgctggc ccggcagaag ggctggttga agccggtgtc cccgtgccgg
    22561 ccgggttgtt gactgccggg tccgaagctg aagttaggcg aacggtccga ccatcctgcc
    22621 gattgcacgc ccacaataag caacttagga ttgtcacact caacaaaatc ccgacgcgcg
    22681 ttccgtggtt agttatgaac ttcatcgctc tcatcattcc cttttccgat catgagttgc
    22741 tgatcaccgg caaccttaca ccgcgtg
//
LOCUS       DASSFQ010000144         8052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1251557, whole genome shotgun sequence.
ACCESSION   DASSFQ010000144 DASSFQ010000000
VERSION     DASSFQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251557"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFH96_05060"
     CDS             <1..749
                     /locus_tag="VFH96_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6973370.1"
                     /translation="LVMMVNQQLVREMFPDENPVGKRITFGGTNTDGQPRWFEIVGVV
                     ANVRSLELREAPGAELYFSAQQDVWPAMSLVVRSSVEPASLGASVRQAVNEVDRSVPV
                     ADVKTMDHVVSESITQPRFNLFLLGLFSAVAMILSTAGIYGVTAYTVTQRTHELGIRI
                     ALGAQVGDVLKMILGQGMAVIGVGLVAGLAAAFGLMRLLRSLLFGVGENDPLTFAAIT
                     GVLVLVALIACYIPARRATKVDPLVALRAE"
     gene            821..3217
                     /locus_tag="VFH96_05065"
     CDS             821..3217
                     /locus_tag="VFH96_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973371.1"
                     /translation="MDSIIKDIRYGLRSLMKRPAFTAVAVITIALGIGVNTAIFSVIN
                     AVLLRPLPYDDPSRLISFRSNQSVLDLTDVESQSKSFSRLGGMVMQPLAYTGGSEPIQ
                     FQVGQVTGGFFETLGVRPERGRFITTEDDKNGAPFVVVLGHDLWVKQFNSDEQIIGKA
                     IPLSGDTYTVIGVMPATFVTPRDSTEAWTPVHVSNPVAANFRGVHFLKTYGRLAPGVS
                     IDQARAEMRIVDQNLAAQYPAENKNRNTVLFALHERIVGETRRSLFILFAAVGFVLLI
                     ACANFANLLLARAAERQREFVIRGALGAGRWRLMRQLLLESLLLSLAGGVAAVVLAFW
                     GTSLLVSFKPENLPRLTEIGVDGRVLGFTLGISILTGLIFGLVPAWAASRGRVGDALK
                     EGGRSSTAGSARQRLRSAFVVAELAVALVLLVGAGLLIKTFWKLRSVEPGFNPDHLIT
                     MRVELPETRYKEVAPQTRFRKQVLAGMNSLPGVQAAMISELPLSGDSLDHDFLIENRP
                     PIAPGDEPSLETRSVLGDYFKVMQIPLKQGRDFGPQDFDEHAPRVGIANDALVQQYFP
                     NEDPIGKRVRWARDPEIHWIEIIGVVGDVKHFGLDLPEQPALYSPYTQINPWKRWMSI
                     AARTQGDSAGAAQALKQEIWKVDSQLPVTRLETMSEVAASSFAARRFNMSLLSLFAGL
                     ALVLAAIGIYGVMSNAVTQRTQEIGIRLALGASTIDVLKLIVRNGLTLVIIGVAVGLA
                     GAFALTRLMTTLLFGVTPTDGLTIVVVSAVLIGVALLACLIPARRATRVDPLVALRYE
                     "
     gene            3252..5672
                     /locus_tag="VFH96_05070"
     CDS             3252..5672
                     /locus_tag="VFH96_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973372.1"
                     /translation="MTKDLLHSIRSLIRHPAFTAVAVITLMLAIGVNTTIFSVVNAVL
                     LKALPFRDPQRLVSVNGTSVNGGLPGIAAYQFLAWKDRNTTFDDLAAFTDNNFNLTGT
                     GEPERIACAQVTSSLFNTLGVKPVRGRIFVAEEDKPGASNVAIVSEAFWQRRFGRDES
                     ILQKTLTLDNKPYAIVGVMPNNFRYPGEFDVWLPLALDPVKELQGDWFQLVDVVGRLK
                     PNATLAGAQSELNLIARQASTQGKEEPLPLAAVEIAPLHKQLVAGIRVTVLVLWGAVG
                     LVMLLACINVASLMISRTLARQREMAVRAAVGARRWQLIRQLLTESVLVGVVGGALGL
                     LIAVWGTRAISSLVPKGFISPVYDLNNIRLDWRVFAFTLGLSVLTGIVFGLAPALTAS
                     RPDLIGALRNSRAHGLMSFGLRSFRGWLVVAELALAVVLLLAAGLLVRSFNKLVAVDL
                     GFDRENVLTARIALPRSSYAKPQQVQAFYDDLLQRVKSLPGVQSAGMINHTPLNGFGL
                     IAFMPVEGYPPLDRKKDPAVGIGTVSPEYFRTMKIPLLSGRFYDARDGADGQKVAIVN
                     QAFAKHFSGNSDPVGKRVGFGCEESEGLCRTIVGVVGNIRQESITDEVAPEIYVPFAQ
                     SRMNSVTLMVRTDSDPLSLARSVRSEVLAIDKNQPVYDVKTLAQRVDEAVAVSRALMV
                     LFASFALLALILGAVGIYGIVSYSVTQRTHEIGIRMALGARAANVLSLIMKNGLTLVL
                     TGIVIGVGSAFGLTRFLATLLFGIEPTDRVTFVVVSATFFVIAMIAALIPALRATRVN
                     PLTALRDE"
     gene            5743..>8052
                     /locus_tag="VFH96_05075"
     CDS             5743..>8052
                     /locus_tag="VFH96_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973373.1"
                     /translation="MSSIIRDVKFALRGLLKHPAFTAIAVVTLALGIGGSTSIFTVVN
                     AALLRGLPYKSPDRLYHIWEQVPKQEFPKREFSYPDYQDYQQNNVFDGLAAYSGGGAI
                     LSGQGDPESLNATRVNAAFFSVLGVDPLLGRTFQNGEDNQGGPKVTVLTYGLWQRRFG
                     ADPNIVGRALTLNGESYTVIGVLPASFQFALRPADLFLPYQPTQNQLMRRFMHGTNLI
                     GRLKSGKTADEAQSELNLIAGRIEQQFNDSHAGVKARVVPLQEEIVGNVRPILLVLLG
                     AVGFVLLIACANVASLLLTRSLARQKEVAIRSALGASRGRVIRQLLTESILLSLAGGI
                     AGLLIAFWGVPALVAVLPQSQLNAMPFLKSLNIDASILAFSFGLSLLTGLFFGLAPAL
                     QSSKLDFNEALKEGGRQTSAGSGHRLRSAMVVSEIALAVVLLIGAGLMMKSLLRLLQT
                     NIGFQTENLLTMTVIAPPSKYTEDNQQINFNDQLRQRVQALPGVASAGTVNILPVNSG
                     NTTRFFIDGDPIPAPGKETEANIRTVSDDYFKTLGVPLLAGRVFDERDTADKPGVVII
                     GKTIADRMFAGRDPVGMRIKYSSIQGQPDLIVGVVGDVKITGIDEALRPVLYYPFRQS
                     SSTFANLVARTNTDPNALAAAIRSEVRNLEPDAAILNVRTMDEMIAQTPASFMRRFPA
                     LLISIFAGIALLLASIGIYGVVSYSVSQQTHYIGVRMALGASPSDILKMILKQGLLLA
                     LLGVAIGVGAAFGLMRLLRTLLYQVSTNDV"
BASE COUNT         1551 a         2164 c         2462 g         1875 t
ORIGIN      
        1 cgctcgtgat gatggtcaat cagcaactgg tgcgggaaat gtttcctgat gaaaacccgg
       61 tagggaagcg aatcacattc ggcggtacga acacggacgg gcaaccacgc tggtttgaga
      121 tcgtcggagt ggtcgcaaac gttcgcagtc tggagttgcg cgaggcgccc ggtgccgagc
      181 tgtacttctc ggcgcaacag gatgtctggc cggcgatgtc gctcgtggtt cgttcgagtg
      241 ttgagcccgc gagtttgggt gcttcagtac gacaggcagt taatgaggtt gatcgatcgg
      301 ttccggtcgc ggacgtgaag acgatggacc atgtcgtcag cgagtcgatc actcagccac
      361 gtttcaatct gttcctgctc ggattgttca gtgccgtcgc catgatcctt tcaaccgcgg
      421 gaatctacgg cgtcaccgct tacacagtca cacaacgcac acacgagctc ggcattcgca
      481 tcgcgctcgg cgcgcaggtc ggggacgtgt tgaagatgat cctgggccag ggaatggcgg
      541 tgattggggt tggattagtc gctgggctcg cggcggcgtt tgggttgatg cgtttgctca
      601 gaagcttgct gtttggagtt ggcgagaatg atccgctgac gtttgcggcg atcacgggcg
      661 tgttggtgct ggtggcactg atcgcctgct acatccccgc gcgccgcgcc accaaggtgg
      721 atccgctggt ggcgctcaga gctgaatgaa aggccatcca cagatttcac agattacaca
      781 gaaagaaaat ctgtgaaatc tgtggatgga tttgatcgat atggactcaa taatcaaaga
      841 catccgttac ggtcttcgca gtttaatgaa gcggccggca ttcacggctg tggctgtgat
      901 cacgattgct ctcggcatcg gtgttaacac tgcaatcttc agtgtcatca acgccgtcct
      961 gctgcgaccg ctgccttacg acgatccatc gcgattgatc agtttcagat cgaaccagtc
     1021 agtgcttgat ctcacagacg tcgagtcgca gagcaagtcg ttcagccggc tcggcggcat
     1081 ggtcatgcag ccgctcgcct acaccggcgg cagcgagccg attcaatttc aggtcggaca
     1141 agtcaccggc ggattctttg aaacactcgg cgtcagacct gagcgcggtc gcttcatcac
     1201 aacggaagac gacaagaacg gcgctccgtt cgtcgtcgtg ttgggccacg atctctgggt
     1261 caaacagttc aacagcgacg agcaaatcat cggtaaggcg attccgctca gcggcgacac
     1321 atacaccgtc atcggtgtga tgcccgcgac gttcgtcaca cctcgcgaca gcaccgaagc
     1381 ctggacgccg gtacacgtct cgaatccggt cgccgcaaat ttccgcggcg ttcatttcct
     1441 gaaaacgtat ggacgactcg cgccgggcgt cagtatcgac caggcacgcg cggagatgcg
     1501 catcgtcgat cagaacctcg ccgcgcagta tcccgccgag aacaagaacc gcaacacggt
     1561 gctcttcgcc ttgcacgagc ggatcgtcgg cgaaacgcgc cgctcgttgt tcatcctgtt
     1621 tgccgcggtc ggctttgtgc tgctgatcgc gtgcgccaat ttcgccaatt tgttgctggc
     1681 acgtgccgcc gagcgccaac gtgagtttgt aattcgcggg gcgctcggtg cgggtcggtg
     1741 gcgattgatg cgtcagttgc tgctcgagag cctgttgtta tcacttgccg gtggtgttgc
     1801 ggcggtggtg ctcgcgttct ggggcacgag cctgctcgtg tcgttcaagc cggagaactt
     1861 gccgcgtttg actgagatcg gtgttgacgg tcgcgtcctt ggtttcaccc tcggtatttc
     1921 gattttgacc ggactgatct tcggtctcgt gccggcgtgg gcggcttcgc gcggtcgcgt
     1981 cggtgatgcc ctcaaggaag gtggccgcag ctcgaccgct ggcagtgcgc gacaacgact
     2041 gcgcagcgcc ttcgtagttg ccgagctcgc ggtagcgctc gtgctgctgg taggcgccgg
     2101 tctgctcatc aagactttct ggaaactgcg ctcagtcgag ccgggtttca atcccgatca
     2161 cctgatcacg atgcgagtcg aattacctga gactcgctac aaagaggttg cgccgcaaac
     2221 acgtttccgc aaacaagtcc tcgccgggat gaattcactg ccaggcgttc aggccgcgat
     2281 gatcagcgag ctgccactca gcggcgactc tctcgatcac gattttctaa tcgaaaaccg
     2341 gccgccgatc gcgcccggtg acgagccgag tttggaaacg cgcagtgtcc tcggcgacta
     2401 cttcaaggtc atgcaaatcc cactgaagca ggggcgtgat tttggaccac aggacttcga
     2461 tgagcacgcg ccgcgcgtcg gtatcgcgaa cgatgcgctc gtgcaacagt attttccaaa
     2521 tgaagatccg atcggcaagc gcgtgcgctg ggcaagggat cccgagatcc actggataga
     2581 aatcatcggt gttgtcggcg acgtgaagca ctttggtttg gacttgccag agcagccggc
     2641 actctactcg ccgtacacgc aaatcaatcc gtggaaacgc tggatgtcga tcgctgcgcg
     2701 aacgcaaggc gattcggcgg gcgcggccca ggcgctcaag caggaaatct ggaaagtcga
     2761 ttcgcagcta ccggtgacgc gactcgaaac gatgagtgaa gtggcggcga gctcgtttgc
     2821 ggcgcggcga ttcaacatgt cgctgctgtc gctgtttgct ggtctcgctc tcgtgctggc
     2881 cgcgatcgga atttacggcg tgatgtcgaa tgccgtcacg caacgaacgc aggagatcgg
     2941 gattcgcctc gcgttgggtg cgagcacgat cgatgtgctc aagctgatcg ttcgcaatgg
     3001 attgaccctc gtgattatcg gtgtggcagt ggggttggcg ggcgcttttg cgttgacgcg
     3061 tttgatgacg acgttgttgt tcggagtgac gccgacggat ggactgacga tcgtggttgt
     3121 gtcggcggtg ttgattggcg tagcgctgct cgcgtgtttg atcccggcgc ggcgagcgac
     3181 gcgcgtggac ccgttagtcg cgctcaggta cgaataggac cggtaggtcc tacaggtcct
     3241 attgaaaaga catgactaaa gatctcctgc acagcatccg gagcttgatt cgtcacccgg
     3301 cgttcacggc ggtggcggtc atcacgctca tgcttgccat cggcgtgaac accacgatct
     3361 tcagcgtcgt gaatgcagtg ctgttgaagg cactaccatt ccgcgatcca cagcggctcg
     3421 tctccgttaa tggaacgagt gttaacggtg gattgcccgg catcgccgcg tatcaattcc
     3481 tcgcctggaa agatcgcaac accactttcg atgatcttgc cgcattcacc gacaacaact
     3541 tcaatctcac tggcactggc gagcccgagc gcatcgcgtg cgctcaggtg acgtcgagtc
     3601 tcttcaatac gctcggcgtg aaacccgtcc gtggccgtat cttcgtggca gaagaagaca
     3661 agcccggcgc gagcaacgtt gcaatcgtca gcgaagcatt ctggcaacga cgtttcggtc
     3721 gcgacgaatc aatcctgcaa aagacactca cgctcgacaa caaaccgtac gcgatcgtcg
     3781 gcgtcatgcc caacaacttt cgctatcccg gcgagtttga tgtttggctt cctctcgcgc
     3841 tcgatcccgt caaagagttg cagggtgact ggtttcagct cgtggacgtc gtcggtcgcc
     3901 tcaaaccaaa cgccacactc gcaggtgcgc aaagtgagtt aaacctgatt gcgcgacagg
     3961 cgtctacaca gggtaaggaa gagcccttgc cgctcgctgc tgttgaaatc gcaccgttgc
     4021 acaagcaact cgtagccggc atacgagtga cggtgctcgt gttgtggggt gcggtcggac
     4081 tggtgatgtt gctcgcgtgc attaacgtcg cgagcctgat gatctcacgc acactcgcgc
     4141 gccaacgcga gatggccgtg cgcgccgcag tgggtgcgcg ccgctggcaa ctgattcgcc
     4201 agcttcttac cgagagcgtt ctcgtcggtg tggtaggtgg cgctttggga ttgctgatcg
     4261 ccgtgtgggg cacgcgcgcg attagcagcc tggttcccaa agggttcatc agccctgttt
     4321 acgatctgaa caacatccga cttgactggc gcgtgtttgc attcacgctt ggtctttcag
     4381 ttctgaccgg aatcgttttc ggattagcac ccgcgttgac tgcttcgcga cccgatctga
     4441 tcggtgcgct gcggaacagt cgcgcgcatg gtctgatgag ttttgggctg cgcagctttc
     4501 gtggctggct tgtcgttgct gaactcgcgc tcgcggttgt gttactgctc gctgcgggtt
     4561 tgctggtgcg aagcttcaac aaacttgtgg ccgttgatct cggcttcgat cgcgaaaacg
     4621 tactcaccgc acgcatcgct ttgccgcgtt caagctatgc gaaaccgcag caggtccagg
     4681 ctttctacga cgacttgttg cagcgggtaa agtcgttgcc gggagtgcaa tcagccggaa
     4741 tgatcaacca cacaccgctc aacggctttg gactgattgc ctttatgccg gtcgaaggat
     4801 atccgccgtt agacagaaag aaggatccag cggtgggaat aggtacagtc tcgcctgagt
     4861 atttccgcac gatgaagata ccgctattga gcggtcgttt ctatgacgcg cgcgacggcg
     4921 ctgacggtca gaaagtcgcg atcgtcaacc aggcgtttgc gaaacacttc tccgggaata
     4981 gtgacccagt cgggaagcgc gtcggttttg gttgtgaaga gtcggaaggt ttgtgccgca
     5041 cgattgttgg tgtggtcggc aacattcgcc aggagagcat tactgacgag gttgcgcccg
     5101 agatttacgt cccctttgcg caatctcgca tgaacagcgt gacgctcatg gtgcgcaccg
     5161 attcagatcc gctctcgctc gcgcgatccg tgcgcagcga agtgctcgcg attgataaga
     5221 atcagccggt ttacgacgtg aagacactcg cgcagcgcgt cgatgaagca gtggctgttt
     5281 cacgcgcgct gatggtgttg tttgcgtcgt ttgcgctgct ggcattgata ttgggcgcgg
     5341 ttggtattta cggcatcgtc tcttactccg ttacccaacg aacacatgag atcggaatac
     5401 ggatggcgct cggtgcgaga gcggcgaacg tgctgagctt gatcatgaag aacgggctga
     5461 cgctcgtgtt gaccggaatc gtgatcgggg ttggtagtgc gtttggattg acgcggtttt
     5521 tggcgacgct gctgtttggg attgagccga cggatcgtgt gacgttcgtg gttgtttccg
     5581 cgacgttttt tgtaatcgcg atgattgcag cgttgatacc ggcgctgagg gcgacacgcg
     5641 tcaatccact gaccgcgctg agagacgaat gaaaggtcgt ccacagattt cacagattac
     5701 acagaaaaag aatctgtgaa atctgcggat ggattggtaa tcatgagttc aataatcaga
     5761 gacgttaagt ttgcactgcg tgggttgctg aagcatcccg cctttaccgc gattgcggtg
     5821 gtcacgcttg cgctcggcat cggtggcagc acgtcgatct tcaccgttgt taatgcggcg
     5881 ttgttgcgtg ggctgcctta taagtcgccc gaccgcctct atcacatttg ggagcaggtg
     5941 ccgaaacagg agtttccgaa gcgtgagttt tcatatcccg attaccagga ttaccagcag
     6001 aacaacgtct tcgatggtct tgctgcttac agcggcggcg gtgcgattct gagtggccag
     6061 ggcgatccgg aaagtttgaa tgcgacgcgc gtgaatgcag cgttcttctc tgtgctcggt
     6121 gtcgatcctc tgcttggacg gacttttcag aacggcgaag acaatcaggg tggtccaaaa
     6181 gtaacagtgc tgacttatgg attgtggcaa cgtcgttttg gtgccgatcc aaacatcgtg
     6241 ggccgcgcgc ttacgctcaa tggcgagagc tataccgtga tcggcgtgtt acccgcgagt
     6301 tttcaattcg cgctgcggcc ggcagacctt ttcctgcctt atcagccgac gcaaaaccag
     6361 ttgatgcgtc gcttcatgca cggcacgaat ctcatcggca ggctcaagtc tggtaagact
     6421 gccgatgaag cgcaatcgga attgaatctg atcgcgggtc gaatcgagca gcagttcaac
     6481 gattcgcatg cgggtgtgaa agcgcgcgtc gtgccgctgc aggaggaaat cgtcgggaac
     6541 gtgcggccga ttctgttggt gctgctcggc gcggttggat ttgtacttct gatcgcgtgc
     6601 gcgaatgttg ccagtttgtt gttgacgcgt tcgctggcgc gacagaagga agtcgcgatt
     6661 cgctcagccc tcggcgcgag tcgtgggcgc gtgattcgtc aattactgac tgaatcgatc
     6721 ctgttatcgc tcgccggtgg cattgcgggt ttgttgattg ctttctgggg cgtgccggca
     6781 ttagttgcgg tcttgccgca gagccaactg aatgcgatgc cgtttttgaa gtcgctgaat
     6841 atagatgcct caatcctcgc tttttcattc ggactttctt tattgaccgg cttgttcttc
     6901 ggactcgccc cggcgcttca gtcttcgaag ctcgatttca acgaagcact caaagaaggc
     6961 gggcgccaga cgtccgctgg ctccggtcat cgtctgcgca gcgcgatggt cgtgagtgag
     7021 attgccctcg cggtcgtgtt gttaatcggt gcaggattga tgatgaagag tctgctgcgg
     7081 ctgttgcaga caaacatcgg cttccagact gagaaccttt tgacgatgac ggtgattgcg
     7141 ccgccgtcga aatacaccga agacaatcag cagatcaatt tcaacgatca actgcgacag
     7201 cgcgtgcagg cgctgccggg tgtggctagt gcggggacag taaatattct tcccgttaat
     7261 tccggtaaca cgacgcgttt cttcatcgac ggcgatccca ttcccgcgcc gggcaaggaa
     7321 acagaagcca acattcgcac cgtcagcgac gattacttca aaactcttgg cgtgccgttg
     7381 ctggccggcc gcgtgtttga cgaacgcgac actgctgaca aaccgggcgt tgtgattatc
     7441 ggtaagacta ttgccgatcg catgtttgcc ggtcgcgatc cggtgggaat gcggatcaag
     7501 tactcgtcga tccagggcca gccggatctg atcgtcggtg ttgtcggtga tgtgaagatc
     7561 accggaattg atgaagcgct caggccggtg ttgtattacc cgttccgcca gagctcttca
     7621 acgttcgcaa atctcgttgc gcgcaccaac accgatccga acgcgctcgc ggctgcgatt
     7681 cgtagtgagg ttcggaacct cgagcccgat gcagcgatct taaatgtgcg cacgatggac
     7741 gagatgatcg cgcagacgcc ggcgtcgttc atgcgacgtt ttccggcgtt gctgatcagc
     7801 atcttcgcgg gcatcgcgtt gttgctcgcg tcgatcggga tctacggcgt tgtctcgtat
     7861 tcggtgagcc agcaaacgca ttacatcggc gtgcgcatgg cactgggtgc gagcccgtct
     7921 gacattctca agatgatttt gaagcaggga ctgttgttgg cgctgctggg tgtggcaatt
     7981 ggcgtcggcg ctgcgtttgg gctgatgcgt ctgttgcgca cactgcttta ccaggtgagc
     8041 acgaacgatg tc
//
LOCUS       DASSFQ010000145         5317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1547066, whole genome shotgun sequence.
ACCESSION   DASSFQ010000145 DASSFQ010000000
VERSION     DASSFQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547066"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..668
                     /locus_tag="VFH96_05080"
     CDS             57..668
                     /locus_tag="VFH96_05080"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET6973374.1"
                     /translation="MKDSEAFILAGGASSRMGADKSQLLIDRQTFTDRIAETLRSLTD
                     SVTIVGRQAHNSELPTVADVYPQWGALGGVHAALAACKREWAIVVACDLPFVTDELFS
                     ALAAVRLDHEAVVPIQPDGRPQPLAALYRVDPCRQRATELIEAGGRRPLDLLEAVKTR
                     WVPFAEIRNLKQAERFFVNINTPSDYYEATHFKVTPPDQTAEK"
     gene            690..1646
                     /locus_tag="VFH96_05085"
     CDS             690..1646
                     /locus_tag="VFH96_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973375.1"
                     /translation="MPPRRRFGKRLVKSFLPIALVIVLAVVGALSFIVYCVSRPAKSA
                     YIVTPESFSKISGRAIKVTDETWPTLDGRRARGWLLRGAEGAPAVVLLHRYGGDRSWL
                     FNLGVKINETTNFTILWPDLRGHGENPPIKWSSLGGREGEDLRAGLDFLRSLKSENQK
                     TLVGESFGVFGIELGAYSALNAARVDPDIKVLVLDSVSNSPAELIRSMVSKCVGVDNS
                     LIQRLGLEGTKLYLMRSYEDESACQFALGMREQRVLLLSGNDAGKLRDSTASLQKCFP
                     VPGNVEVRTDLPLSGFSLPSATGEQGEAYDRIVIEFFARNLR"
     gene            complement(1734..3155)
                     /locus_tag="VFH96_05090"
     CDS             complement(1734..3155)
                     /locus_tag="VFH96_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6973376.1"
                     /translation="MHPSTLAKRVYLLLFAIIVVFYFYGLGKLPLLGPDEPRYAQVAH
                     EMFLNHDLITPTLGGHTWFEKPALLYWMIVLAFKVFGVSEWSARLGPAVCGLLTVIAL
                     WFVAREVDAPKFAFWAIVVSATCLGLIVFSRAASFDVVITMTSTWSLAFFLLYELPSR
                     RNKRLLLAGFYVFVGLSLLAKGLVGIVIPFGVVGFYFVLRRAWPQRSVWLSLFWGVPL
                     ALLVSATWYGPVIARHGWTFIDEFFVQHHFARFVSNKYHHPQPFYFYLAIILMLALPW
                     TVHLIVALVRARKWQWRAADSVSIVRVFALAWLVLPILFFSFSGSKLPGYILPAVPAV
                     ALLVADRLTVAQKARWPLLIAGATVGLVVIVLNFAGPFARRESVKDLLALADARGYAN
                     APVIAQRGDDRSAEFYASGRVIYRADGEVLTFDEISVDDARARSGKFVVFIPVQYVEN
                     YRGAPKIEIIGDNGHTAVLGWQP"
     gene            3207..4196
                     /locus_tag="VFH96_05095"
     CDS             3207..4196
                     /locus_tag="VFH96_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6973377.1"
                     /translation="MLRTAVIGVGSLGRQHARIHADLASEGASEFVAVCDLNEETARS
                     ISGERKTDWTTNWRDLVGRIDAASIAVPTEAHCEIACGLLEAGIHVLVEKPISRTLEE
                     ADRMIEAAARGNAMLQVGHLERFNPAMVALRPHVRKPVYFEIHRVGQFTARSLDIDVV
                     LDLMIHDLDIVQWLVGEDVEVTNIHAVGIAILTNKVDAANARLEFSSGAVANITASRV
                     GTEKIRKMRFFQPHDYIAIDYTTKRATVSSLSPPAAGSSWPGVHINHLDIVDVEPLRA
                     EIVSFLESAREGTPPAVSGLEGRNALSLALRTLERIREHAEHPGMSELSANVR"
     gene            4209..>5317
                     /locus_tag="VFH96_05100"
     CDS             4209..>5317
                     /locus_tag="VFH96_05100"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6973378.1"
                     /translation="MSTANEQLSALLQQHIETGEFPSAVYLVAERDEILYTDALGYSV
                     VEPYRVANKLDTIYDLASLTKPLITALLCARRIELGELTLDSSVSHYLPEFDRTDKQM
                     ITVRELLTHTSGLPAWRPLYILTEEEPERAAGAIANLDFEYKPGTRVVYSDLGFIALG
                     ILLERLTGHRFAELAQREIFEPLNLKQTFFNPEVALQTGIAACETGNAYEAGMCKQME
                     VGEYANSRQRVIWGEVHDGNAYFLGGAAGHAGLFSTASETFVLAQQFLSESTKLLAPE
                     TCKLFREDMTPNLEEARSIGWQLAATKDSTGGAELPRDSFGHNGFTGTCVWIDPNHRR
                     VFILLTNRTHARALPFANINAVRREFNSLAIKALT"
BASE COUNT         1290 a         1492 c         1446 g         1089 t
ORIGIN      
        1 cggtcaaggt ttccacagat tacgcagaaa acgcagattc tgtgtaagct gtgttcatga
       61 aagattcgga agctttcatt cttgctggcg gcgcctctag cagaatgggc gccgacaaat
      121 ctcagttgct gattgaccgc caaacgttta ccgaccgaat cgcagaaaca cttcgctcac
      181 ttactgattc ggtcacaatc gtcggccgcc aagcgcacaa ttcagagctc ccgaccgtcg
      241 cggacgtcta tccgcagtgg ggagcgctcg ggggtgttca cgccgcgctc gcggcttgca
      301 agcgcgagtg ggccatcgtc gttgcctgtg atttgccttt cgtcactgac gagctgttct
      361 cggccctcgc tgctgtgagg ttggaccacg aagcggtagt tccaattcag ccggatggcc
      421 gcccgcaacc attagccgcg ctctatcgcg tcgatccttg ccgccaacgc gcaaccgagc
      481 tcattgaagc aggcggccgc aggcctctcg acctcctcga agcagtcaaa acacgctggg
      541 ttccgtttgc cgaaataagg aatctcaaac aggcggaaag gttttttgtt aacatcaata
      601 cgccgtcaga ctattatgag gcgacccatt ttaaggtgac tccaccggac cagacagcag
      661 aaaagtgaaa tccagggcta ctcacacaaa tgccaccgcg aagacgattt ggtaaaagac
      721 tcgttaagtc tttcctgcca atagcactgg tgatcgtgct ggctgtggta ggcgcgctct
      781 cttttattgt ctattgcgtt tcccgtcccg cgaagagtgc ttacatcgta acgccggaat
      841 cgttttcaaa gattagcggt cgcgcgatca aggttaccga tgagacctgg cccacgctcg
      901 acggccgtcg tgcgcgcggc tggctattgc gcggcgcaga aggcgcgccg gcagttgtgc
      961 tacttcatcg atatggcggc gatcgctcgt ggctgttcaa cctcggcgtc aagattaacg
     1021 agacaactaa cttcacgatt ctctggcctg accttcgtgg ccatggcgag aatccgccga
     1081 tcaaatggag ttcgctcggc gggcgtgaag gcgaggacct gagagctgga ctcgacttcc
     1141 tccgcagtct caagtcagaa aatcagaaga cgctcgtggg cgagagtttc ggagtgtttg
     1201 gcattgagct cggtgcttac agtgcgctga atgcggcacg cgtcgatccg gatataaagg
     1261 tgctggtgct cgactccgtt tcaaactcac cggctgaact gatcagatcg atggtctcga
     1321 agtgcgtggg cgttgataac agtttgatcc aacggttggg gctcgaggga acgaagctct
     1381 atttgatgag aagctacgaa gatgagagcg cctgccaatt cgcgttgggc atgcgcgaac
     1441 agcgtgtgct gttgctttcc gggaacgatg cgggaaaact gcgcgactcg acggcgtcgt
     1501 tgcagaagtg ttttccggtg ccggggaatg tcgaagtgag aactgatttg ccgttgagtg
     1561 gatttagtct gccgtcagcg actggtgaac agggtgaagc ctacgaccgg atcgtgattg
     1621 aattcttcgc ccgcaatctc aggtaaaagc catggacagg attaagaaga tttctcagga
     1681 tatactggat aaatctggca catcttgaga catcttctta atcctgtcca catttacggt
     1741 tgccacccaa gtaccgcggt gtggccattg tcgccaataa tctcaatctt cggcgcgcct
     1801 cggtaattct cgacgtactg caccggaata aacacgacaa actttccact ccgcgcccgc
     1861 gcatcgtcga ctgagatctc gtcaaacgtc agcacttcac catcagcacg ataaattact
     1921 ctaccggaag cataaaactc cgccgatcga tcatcacctc gttgcgcaat caccggcgca
     1981 ttcgcatacc cacgcgcatc ggcaagcgca agcaaatctt tgactgactc acgtctcgcg
     2041 aaaggcccag caaaattcaa aacaatcaca accaacccta cagtcgcacc cgcgatcagc
     2101 aacggccatc gcgctttttg tgcgaccgtc aaacgatcgg cgacgagcaa cgcaaccgcc
     2161 ggcacggcag gaagaatgta tcccggcagt ttcgagccgg aaaatgagaa aaagaggatc
     2221 ggcaacacca accacgcgag cgcgaagaca cgaacgatgc tcacgctatc ggcggcacgc
     2281 cactgccact ttcgtgccct gactaacgca acgatcaaat gaacggtcca aggcaatgca
     2341 agcatcagga taatcgcgag gtaaaaataa aacggctgag gatggtggta cttattcgaa
     2401 acaaaacgcg caaagtgatg ctgtacaaaa aactcgtcga taaacgtcca accgtgtcgc
     2461 gcgatgactg gtccatacca ggttgctgag actagcagtg cgagcggcac accccagaaa
     2521 agactcagcc acactgaacg ttgcggccac gcacggcgga gcacgaaata gaatccgacg
     2581 acgccaaacg ggatcacaat cccaaccaga ccctttgcga gcaacgataa gccgacgaac
     2641 acgtaaaagc ctgccaaaag caaccgcttg tttcgcctcg acggcagttc gtataacaga
     2701 aagaacgcga gcgaccaggt ggatgtcatc gtgatcacga catcaaaact ggccgcccgt
     2761 gaaaacacga tcagcccgag acacgtcgcg ctaacgacga tcgcccaaaa cgcaaatttg
     2821 ggcgcatcga cttcacgcgc cacaaaccaa agagcgatca cggtcagcaa cccgcagacc
     2881 gccggtccaa gccgcgccga ccactcacta acgccaaaca ccttgaacgc caagacgatc
     2941 atccagtaaa gcagcgccgg tttctcaaac cacgtgtgac cgccgagtgt tggcgtaatc
     3001 aaatcgtgat tgagaaacat ctcgtgcgct acctgcgcgt agcgcggctc gtctggtcca
     3061 agtagcggca gtttgccgag tccgtaaaag taaaagacga cgatgatggc gaagagtaaa
     3121 aggtagactc gcttggcaag cgtcgaagga tgcatgtctt aatgaatctg tgtaatctgt
     3181 ggataaaggg ctcagactaa cacataatgt tacgcacagc agtcatagga gttggcagtc
     3241 tcggacgaca acatgctcgg attcatgccg atctcgccag cgaaggcgct tcggaattcg
     3301 ttgcagtttg cgatctaaac gaagagacgg cgcgctcaat cagtggtgaa cgaaaaaccg
     3361 actggaccac caactggcgc gatttggtcg gacgcattga tgccgcgagc atcgccgttc
     3421 cgactgaagc acactgcgag atcgcgtgcg gactgctcga agcgggcatt cacgtcctcg
     3481 tcgaaaaacc gatctcgcgc acgcttgaag aagccgatcg catgatcgaa gccgcggcac
     3541 gcggcaacgc gatgctgcaa gtcggacatc tcgagcgttt caatcccgca atggtcgccc
     3601 tgcggccaca cgttcgcaag cctgtctatt tcgagatcca tcgcgtgggc cagtttaccg
     3661 cgcgttcgct cgacatcgac gtcgtactcg atctgatgat tcacgatctc gacatcgtgc
     3721 aatggctcgt cggcgaagac gtcgaagtga caaacattca cgctgtcggg atcgcgattc
     3781 tcacaaacaa ggtcgacgcg gcaaacgcgc ggctcgaatt ctcatccggt gcggtggcga
     3841 acatcaccgc gtcgcgcgtg ggaactgaga agattcgcaa aatgcgtttc ttccagccgc
     3901 acgactacat cgcgattgat tacaccacca aacgcgctac ggtcagcagt ctctcaccgc
     3961 ctgctgcagg tagctcgtgg ccgggtgtgc atatcaatca tctggatatc gtcgacgtcg
     4021 agccattgcg tgcggagata gtctcgtttc tggaatcggc gcgcgaggga acgccgccgg
     4081 ccgtctccgg acttgaaggc cgcaatgccc tgagtctggc actgcgcact ctcgaaagaa
     4141 tccgcgaaca cgccgagcat ccgggcatga gtgaactaag cgcgaatgtt cgctagactg
     4201 ttcttgcaat gtctacagcg aacgagcaat tgtcggcgtt acttcagcaa cacatcgaga
     4261 cgggtgagtt tccttcagcg gtttatctag tcgccgaacg cgacgagatc ctctataccg
     4321 atgcgctcgg gtattcggtt gtcgaacctt accgcgtcgc aaacaaactc gacacaatct
     4381 acgatctcgc ctcactcaca aaaccactga tcactgccct gctctgcgcg cgccgcatcg
     4441 aactcggcga gctaacgctc gacagctcag tttcacacta tctgccggaa ttcgatcgca
     4501 ctgacaagca gatgatcaca gtgcgcgaat tgctgacaca cacttcaggt ctgcccgcat
     4561 ggcgtccgct ttatatcctg accgaggaag aacccgagcg tgctgcgggc gcgatcgcga
     4621 atcttgattt tgaatacaaa cccgggacgc gtgtggtcta cagcgatctc ggtttcatcg
     4681 cgctgggtat tttgctggaa cgtctgaccg gacatcggtt cgccgaactg gcgcagcgtg
     4741 agatcttcga accgctaaac ctgaagcaga ctttcttcaa tcctgaagtc gcgctgcaaa
     4801 caggtatcgc tgcgtgcgag acgggcaatg cgtatgaagc cggcatgtgc aaacagatgg
     4861 aagtgggcga gtatgctaat tcacggcagc gcgtcatctg gggtgaagtt catgacggga
     4921 atgcgtactt cctgggcggc gcagcggggc acgccggatt attttcaacc gcgagcgaga
     4981 cgtttgtgct ggcgcaacag tttctgagtg agtcgacgaa gctgctcgcc ccagagacgt
     5041 gcaaactgtt tcgagaagac atgaccccga atttggaaga ggcgcgttcg atcggttggc
     5101 aattggcggc gacgaaagat tcaacgggcg gagctgaact gccgcgggat agttttggtc
     5161 acaacggttt tactggcacg tgcgtctgga tcgatccgaa tcatcgtcgc gtgttcattc
     5221 tcctcactaa tcgcacgcat gcacgtgcgc tgccgtttgc gaatatcaat gcggtacgac
     5281 gggagtttaa ttcactggcg atcaaggcac tgacata
//
LOCUS       DASSFQ010000146        16195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_212520, whole genome shotgun sequence.
ACCESSION   DASSFQ010000146 DASSFQ010000000
VERSION     DASSFQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16195
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212520"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFH96_05105"
     CDS             <1..1098
                     /locus_tag="VFH96_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6973379.1"
                     /translation="LRAFAFEQLGRTEDAINGYLSLPDGRNEYYGTRATQRLLDLSTN
                     EKSRKLVQMRLNALLNESKTASAAGQFEQSRAAAQSSLRLTNDPQTRAEALKYLQTAY
                     NGLPTYRLPTFNKIPLLKQDPELGQHEALANSLLLLGLYDEALPEFLAARKVSAGSDE
                     DYSIAVLSLRAGIPNRAVRFGEQIWKNMPADYVIELAPRELVELLYPAPFRESLLKHT
                     SSRNVDPRFILSIARQESRFQADAKSIAAARGMMQFIASTANEIATQVDFQNFNQDDL
                     YNADTAILFGSQYLSNLFKQFPNQPQAVAGSYNGGADNLARWIARSRANEPDRYVPEI
                     GFSQTKDYVYKVMANYWTYQRLYDAQLQATK"
     gene            1146..1964
                     /locus_tag="VFH96_05110"
     CDS             1146..1964
                     /locus_tag="VFH96_05110"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET6973380.1"
                     /translation="MSLTLCFGVTCRHSNQKRFDFKGKVVIVDQEKHLVTVSHEDIKG
                     YMPGMTMPFAVKNEADLQILAPNDEITATLVVDGKHSWLEDLIITRQSATAPAMPGAQ
                     MAKEGDEVPNFTLRNQDNREIHIKDYRGKTLLLTFIYTRCPVPDYCTLMSDNFAQIDR
                     ALGENQELYGKTHLLSISIDPEYDTPKVLRSYGAAHTERYQNETFAHWEFAGGTKEQV
                     KDIAQFFGLTYFPEKDQIIHALRTVIVNPDGKVAKIYGGNDWKPEEIVVAMQKQ"
     gene            complement(2016..2999)
                     /locus_tag="VFH96_05115"
     CDS             complement(2016..2999)
                     /locus_tag="VFH96_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET6973381.1"
                     /translation="MSNGLLVFIEHKGGTANRSSVEAIAAAQALGSELGQRVSAVVLG
                     ADVAGLAQEIAGYDLEKVICATNPKLAEYTPDAYADGMEQIVKQVDPNFVFLTHTYQV
                     RDFAPKLAARFGKSLISDCIRAKAEGGKIGFTRRIFLGKLDADVVSDGEAPVFATFQT
                     GAYRPDQAAKGSGAPVENVAVEIGEVRMKPEAPFQEAKQAVDLTKAEVIVAVGRGIKS
                     KDNLALAEKLAEVLGGDLAASRPICDAEWLPIDRQIGSSGQTVAPKLYVALGISGAIQ
                     HLVGMKNSGTIVAINKDPEAPIFDIADYGIAGDLFEAVPVLTEEIKKIKGI"
     gene            complement(3015..3788)
                     /locus_tag="VFH96_05120"
     CDS             complement(3015..3788)
                     /locus_tag="VFH96_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET6973382.1"
                     /translation="MKIIVMMKQVANKDAILRINKDATWIEEGDLSFEVSESDGYALE
                     EALRVREKLGGEVVVCSMGPQRVKSVIKDALARGADRAIHVVGDNLTHLTPYAAATIL
                     AGAIRDEKPDLVMTGLQSDDHGYGQTGVIMAELLGLPHATIVIELDAAADKLRVKREL
                     ESGWYQWYEMPLPALLTIQSGISQIRYATLKGIMAAKKKEIKEVTPPAELVERPSHQR
                     VEKIYLPQKMKQTQMLGNGDAKAGAAELAQKLHVEARVY"
     gene            complement(3788..5047)
                     /gene="fabF"
                     /locus_tag="VFH96_05125"
     CDS             complement(3788..5047)
                     /gene="fabF"
                     /locus_tag="VFH96_05125"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET6973383.1"
                     /translation="MTRRVVITGLGLVTPVGNTVEDTWASLMAGRSGAGYIEKFDAEK
                     FSVRFACEVKNFDPLAFIPKKDARKMGAFIHYAIAASIEAVADSGIQLTPEGKMPADI
                     AENVGTYISSGIGDFWAIEREHSKLLDDGPDRVSPFFIVSAIVNLAAGQVSIRFGAKG
                     PNSATATACAAGAHAIGDSFKIIQRADADIMICGGAESAITPMSVAGFSAMRALSTNN
                     DDPVHASRPFERDRDGFVIGEGAGIMILEELESAKRRGARIYAEIVGYGASADAFHLT
                     MPDETGSGARRVMQRTLKDAGVSPEQVGYINAHGTSTPYNDKFETLAIKETFGAHAYK
                     LAVSSTKSMTGHLLGAAGGIESVFSVLTLYRNVIAPTINYVNPDPECDLDYVPNEPRE
                     ARVEYVLSNSFGFGGTNAALLFKRYEG"
     gene            complement(5141..5383)
                     /locus_tag="VFH96_05130"
     CDS             complement(5141..5383)
                     /locus_tag="VFH96_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6973384.1"
                     /translation="MPDQEIENKVKQIIVDELGVDENEVTPNARFIDDLGADSLDTVE
                     LVMRFEEEFGIEIPDEDAEKIQSVRDAYNYIDQHKK"
     gene            complement(5413..6354)
                     /gene="fabD"
                     /locus_tag="VFH96_05135"
     CDS             complement(5413..6354)
                     /gene="fabD"
                     /locus_tag="VFH96_05135"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET6973385.1"
                     /translation="MTQNNLRNLRMAFVFPGQGSQSPGMGKDLAENFPVARQVFEEAD
                     DALGFAISRLCFDGPAEDLQLTENTQPAILTVSVAAFRAMQETGVDAPAFVAGHSLGE
                     YSALVAAGALSLTDAVRTVRARGRYMQEAVPVGTGAMAAVIGGDLEAIERICAEARGD
                     QVCSIANINAPNQVVIAGNTEAVDRAVELLSGVARKVVKLKVSAPFHCALMKPAQDRL
                     ALDLAALNFSGPAMPVVTNVDARATKAPDELRDALVRQVSAPVRWVESMQLLIEQGVE
                     RLVEVGPGKVLSGLMRQISRDVKMLNVEDAASLQQIE"
     gene            complement(6362..7348)
                     /locus_tag="VFH96_05140"
     CDS             complement(6362..7348)
                     /locus_tag="VFH96_05140"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET6973386.1"
                     /translation="MLNQRAGILGTGHSYPEGILTNEELERIVETSDEWITTRTGIKQ
                     RRKAAPDEYTSMFAVRAARQAIERARLDPADIDLLLCATVTPDQILPSTGCIVQAALG
                     TNNAAAMDIVAACSGFLYGVSLADSMIRTGQVRHAVVVGAEILTQYVDYSDRQTCVLF
                     GDGAGAAVLGPVENGRGILASKIKSDGRYEEQLFSPGGGTRKKPTAETLEAGDHYFKM
                     RGNELFKVAVRSMAEISREVLEQAGAKSDDISLFIPHQANQRITEAVADRLNVDMSRV
                     YSNIAVHGNTSSASIPIALDECVSAGRIKPGDLVLMASFGGGATWGAVLMQW"
     gene            complement(7489..8553)
                     /gene="plsX"
                     /locus_tag="VFH96_05145"
     CDS             complement(7489..8553)
                     /gene="plsX"
                     /locus_tag="VFH96_05145"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET6973387.1"
                     /translation="MRSQSQHQPQLTGKVVLDAMGSDHAPNVEIDGALAATRDFGVGV
                     ILVGQAHKVEPELRRCGWRGDGDRGIELVDAAEVIAMDDPVATSVRRKKKSSLRIGSK
                     LVMDGRGDGFVSAGNTGAAMATAKMVIGTLPGVDRPALAAMIPTKAAKPTLLLDVGAN
                     SECKPHHLAQFAIMGDAYSRSVLGTARPSVGLMSIGEEEAKGNELTKEAFPLLRQLSD
                     INFVGNVEGRDVFSGLVDVIVTDGFTGNVMLKLSEGLTEAMLSMLKRELMSTAVTKAG
                     AMLAKPAFRSIKKRLDYTEYGGAPLLGVSRIVVIGHGRSNARAIRNAIRSVKEFSEHR
                     TAQRIEEGINHPQITQRYTA"
     gene            complement(8562..8744)
                     /gene="rpmF"
                     /locus_tag="VFH96_05150"
     CDS             complement(8562..8744)
                     /gene="rpmF"
                     /locus_tag="VFH96_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET6973388.1"
                     /translation="MPNPKRRHSKARTAKRRAHDALRVPQTTLCPNCREPRMPHRVCP
                     KCGFYKGREVVHVEQS"
     gene            complement(8833..9357)
                     /locus_tag="VFH96_05155"
     CDS             complement(8833..9357)
                     /locus_tag="VFH96_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET6973389.1"
                     /translation="MRIELENLEGSKGDFAHVYQPDELNPVDDRVSLIAPAAVNGKVR
                     LSGNEVFVNGHVETRAQVECDRCLKPVELPVNADFTLEYMSGSDYESSQVAELTEAEM
                     LVSVFDGAAIDVDEIVKEQILLAVPTRMLCREDCKGICPECGTDRNTGECNCVTNDID
                     PRWAALKNLTKNLT"
     gene            complement(9387..10622)
                     /locus_tag="VFH96_05160"
     CDS             complement(9387..10622)
                     /locus_tag="VFH96_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973390.1"
                     /translation="MSTPAGSVEKAVVGFTNIKGSRMRPGISHYCQKCLAANALGQEF
                     CTRCGTRLMIVVEPSSTRFETAPTSLSTDEHLLERISAAENRVARLAERLERSLDLLL
                     RYAQNAYFDRSLMRALVGLLTEDGVIETERLERMWSERCQRDTVEQEENLHRDALRLR
                     ILATAQVPDKQVFEGLVNEGFVLIEDRQVPQGMSKLQAAAEIAAENAPLNLFIGEHYF
                     RRGKTKQARAYLAKAHAAMPDDLRISLLLGLTCADDGEVELAKDLLSSATAQGCSSFA
                     GHCGLGWVFVAEKNWRRALGEFKRALTVRPSAEAHYVLGCLYYELNRDTLAVRHLRKA
                     TEMDGNYAEAFSLLAQAYERTGRKELARQALEKAGRKNGSLFQVTKSGAMRLMTGADK
                     RLAEALREDALSADFTNGH"
     gene            complement(10567..11442)
                     /locus_tag="VFH96_05165"
     CDS             complement(10567..11442)
                     /locus_tag="VFH96_05165"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HET6973391.1"
                     /translation="MNPANSENPANPVTIGVVGAGTMGNGIAQVSARAGYNVIMHDVR
                     DEFLDRGLKAIDKSLQRDVDKERLDGAGKKAVIEKIRTTTDFAAFKEASFVVEAVVED
                     LGVKTQVFKMLDEVTPPHAILASNTSSISITKLGAATKRPDKVIGMHFMNPVPVMKLI
                     EVIRGMATSDETYERVRTLSEQLGKTALDCQDSPGFVSNRVLMPMINEAIFTLYEGVA
                     SRESIDGIMKLGMNHPMGPLTLADFIGLDVCLAIMNVLYEGLGDPKYRPCPLLKRYVD
                     AGWLGRKSGRGFYEY"
     gene            complement(11475..12662)
                     /locus_tag="VFH96_05170"
     CDS             complement(11475..12662)
                     /locus_tag="VFH96_05170"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET6973392.1"
                     /translation="MPELNEAVIISAARTPVGKFLGSLKGFTAPELGAIVVRESVKRA
                     GIKPEDVDEVIMGCVIQAGLGQNPARQAALNGGIPSTVAAVTVNKVCGSGLKAVMMAA
                     QGVQLGDSEIVVAGGMESMSNAPYLIPKAREGYRLGNGELIDSMIHDGLWCAFENYHM
                     GCTGEVVADEYKIGRTEQDEFATSSHRKAAAAIKAGKFKDEIVPVEIKQKKGAALIFD
                     TDETVREDTTVEVLGKLKPAFKSEGTVTAGNAPSVNDGASAVVVTSLRRARELGIEPM
                     AKIVAQAVSGVEPRLVMMAPVEAIRKLLKKTGWSGSEVDLVELNEAFSVAALAVTREL
                     GLDPEKVNVNGGAVALGHAIGQSGSRLLTTMLYELKRRDARRGIVALCLGGGNAVAMA
                     IER"
     gene            12724..13758
                     /locus_tag="VFH96_05175"
     CDS             12724..13758
                     /locus_tag="VFH96_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET6973393.1"
                     /translation="MQALILAGGKGTRLRPLTVYTPKPIVPICNRPFLLYQIDTLRRA
                     GITDITLSLSYQPNKIEQLLGDGSDFGVKLKYTVEPQPMGTAGAYKFAEDLIREPTVV
                     FNGDILTDLDLKAVIREHTERKATATIVLAPVENPQSYGLVETEKDGRIRRFLEKPKA
                     DEITCNTINAGTYILEPKMLDYLPAGENYSFEYGLFPDLLKRGEAFYAHIPVRTYWID
                     IGTPERYLQVHDDMLANRVSRFHIKERRGQFDAATVAEIDELSMIGDDCQIKPGAQII
                     NSVLGQGCFVEERARVENSVIWPHTRIGTTAQVSGTIVGRGCHIGRSAIVRPGTVLGD
                     KTSLTDYTFA"
     gene            13817..15169
                     /locus_tag="VFH96_05180"
     CDS             13817..15169
                     /locus_tag="VFH96_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET6973394.1"
                     /translation="MNWDVFRAYDIRGTYPDQINEEDYYRTAKAYVYLFKPKTMVVGM
                     DARVSSPVLKAGLTRGFLDAGVDVVDIGQITTDMLYYTVGAHSEYSGGIVVSASHNPV
                     QYNGMKMVREKATAISSDTGLFDIRDTLKAGKDAEVTSDKKGTCTERDILNDYLEHVL
                     KSIDRTKIKPFKFVGNANFGYVCRPAAKLAKDLNLTLLPLNFEPDGTFPKGPPDPMLP
                     GNRTETESLIKEKGASFGVAWDADADRAMFFTEKGEFINGAYITAFLADILLTKYGSD
                     NTIIFDPRVIWPTLDAVKRHGGRPIISKGGHAFMKDRMRKENGLFAGEMSGHYYFREN
                     FYADNGVIPFLLVLEHLSTHDKPFSELIAPFMAGHYMSGELNYHVKDIKAVIEKVQEK
                     FHGEGTEDFTDGYSLETAEWRFNIRGSNTEPLLRLNIEARKDGLVDHIRQEIEKLIES
                     "
     gene            15240..16067
                     /gene="mutM"
                     /locus_tag="VFH96_05185"
     CDS             15240..16067
                     /gene="mutM"
                     /locus_tag="VFH96_05185"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET6973395.1"
                     /translation="MPELPEVEHVVRALSPVVTGRRILAAELNLKRIAPGISRPAFNR
                     QLQNALITGVGRRGKYILFKLETGHVLTTHLRMTGKFVSLTIDESLPPYAHVVFHLDD
                     DRRLVYCDMRQFGRMQIFKDPLRPPKELQTLAPEPLSDEFTEEYFLNTLKRSGRPLKH
                     LLLDQTRVLGLGNIYAVEALFLAGVSPMKAANKLSKPRARKLYQAIRDVLQEAIDAGS
                     TLKIDLADGNSSYVGTSERFWRVYEREGEPCVRCGTKIKRIVQGGRSTYYCPKCQKN"
BASE COUNT         3578 a         4897 c         4121 g         3599 t
ORIGIN      
        1 ctgcgcgcgt tcgcgttcga acagctcgga cgcactgaag acgcgatcaa tggctatttg
       61 tcccttccgg atgggcgcaa tgagtactac ggcacgcgcg cgacgcagcg attgttggat
      121 ttatcgacga acgagaagtc acggaaactc gtgcagatgc ggctcaatgc tctgctgaat
      181 gagagtaaga ctgcgagtgc tgcgggacag tttgagcaat ctcgcgcggc agcgcagtct
      241 agtttgcgcc tcacaaacga tccgcagacg cgagcagaag cgctgaagta tttacaaacg
      301 gcgtacaacg ggctgcccac atatcgtctg ccaaccttta acaagatccc tctcctaaaa
      361 caggatcctg agctgggaca gcacgaggcg ctggcgaaca gcttgctgct tctcggtctc
      421 tacgacgaag cgcttcccga gttcctcgcg gcgcgcaaag tgtctgccgg ttcggacgaa
      481 gactactcaa tcgccgtgct ctcgctgcgt gcgggtattc cgaaccgggc ggtccgtttt
      541 ggcgagcaga tctggaaaaa tatgcctgcc gactacgtga tcgaattagc gccacgcgag
      601 ctcgtcgagt tgctttaccc agcgccgttt cgcgaatcgt tgttgaaaca cacatccagc
      661 aggaacgtcg atccgcgttt tatcttgtcg attgcgcggc aggagtcgcg cttccaggcg
      721 gatgcaaaat cgatcgcggc cgcacgaggg atgatgcagt tcatcgcttc aaccgcaaac
      781 gagattgcga cccaagtcga ttttcaaaac ttcaaccagg acgatctcta caacgcggat
      841 acggcgatct tgtttgggtc gcagtatctc tcgaatttgt tcaagcagtt tccgaatcag
      901 ccgcaggctg tggccggttc gtacaacggc ggcgctgaca acctcgcgcg ctggatcgca
      961 cgctcgcgcg cgaacgagcc tgatcgatac gttcccgaga tcggtttctc gcaaacgaag
     1021 gattacgtct acaaagtgat ggcgaattac tggacgtatc aacgcctcta cgacgcccaa
     1081 ctacaagcca caaaatgatc aagccacaaa aaggcacaaa aagcacaaaa gttatcttct
     1141 tgtttttgag tctcactctt tgttttggag tgacgtgcag gcacagcaat cagaaacggt
     1201 tcgacttcaa aggcaaggtg gttattgtcg accaggagaa gcatctcgtg acggtttcgc
     1261 acgaggacat caagggctac atgcccggga tgacgatgcc gttcgccgtc aagaatgagg
     1321 cggacctgca gatactcgca ccgaacgatg agatcacggc gacgctcgtg gtcgacggca
     1381 agcattcctg gctcgaagat ctgatcatca cgagacagag tgcgaccgcg cctgcaatgc
     1441 cgggcgcgca gatggcgaag gaaggtgatg aggttccaaa cttcacgctt cgcaaccagg
     1501 acaatcgcga gattcacatc aaggactatc gcggcaagac gttgttgttg accttcatct
     1561 acacgcgctg tcccgtgcct gattactgca cattaatgtc cgacaacttc gcgcagatcg
     1621 atcgcgccct cggagaaaat caggagcttt acggaaagac gcacttgctc agcatcagca
     1681 tcgatcccga gtacgacaca ccaaaagtgc tgcgcagcta cggcgccgca cacaccgagc
     1741 gttaccagaa cgagacgttt gcgcactggg aattcgcggg aggaacaaag gagcaggtga
     1801 aagatatcgc gcagttcttc gggctaacct attttccgga gaaggatcaa atcattcacg
     1861 ctctcaggac ggtgatcgtg aatcccgatg gaaaagtcgc gaagatctac ggtgggaacg
     1921 attggaaacc cgaggagatc gtggtagcaa tgcaaaaaca gtaacgcgga taaacgcgga
     1981 tttgaatccg gatttttatc cgcgtaatcc gcgttctaaa ttccctttat ctttttgatc
     2041 tcttcggtga gtacgggcac cgcttcgaaa agatcgccgg caatgccgta gtcagcgata
     2101 tcgaaaatcg gcgcctcagg gtctttgttg attgcgacga tcgttcccga gttcttcatg
     2161 ccgacgaggt gttggatcgc tccggagatg ccgagcgcaa cgtaaagctt cggcgcgacg
     2221 gtttgccctg aggatccgat ctggcgatcg ataggaagcc attccgcgtc acagatcgga
     2281 cgcgaggcag ccagatcgcc gccgagcact tccgcgagtt tctctgcgag cgcgagattg
     2341 tctttactct tgatcccacg cccgaccgca acaatcactt cagccttcgt gagatccacc
     2401 gcctgcttcg cttcctggaa cggagcttca ggcttcatgc gcacctcacc gatctcgacc
     2461 gccacattct caaccggcgc accgcttccc ttcgccgcct gatccgggcg ataagctccg
     2521 gtctggaacg tcgcaaacac cggcgcttca ccgtcagaca caacatctgc atcgagcttc
     2581 ccgagaaaaa ttctgcgtgt gaacccgatc ttcccacctt ctgctttcgc gcgaatgcag
     2641 tcgctgatca acgacttgcc aaaccgtgcc gcaagctttg gagcgaagtc acgcacctgg
     2701 tacgtgtgcg tcaggaaaac gaaattcgga tcgacctgct tgacgatctg ctccatgccg
     2761 tcggcataag catccggtgt gtattcagcc agcttcgggt tcgtcgcaca aattactttc
     2821 tccagatcgt aacccgcgat ctcctgcgct aacccggcga catccgcgcc gagcacaacc
     2881 gcactaaccc tctggcccaa ctcggatccg agcgcctgcg ccgccgcaat cgcctcaacg
     2941 gaactgcggt tcgccgttcc acctttgtgt tcgataaaga ctaataatcc gttactcatc
     3001 cgtgtaatcc gtggctaata aactctcgct tcaacatgaa gcttctgggc caactccgcc
     3061 gccccagctt tcgcatcgcc atttccgagc atctgcgtct gcttcatctt ctgcggcaag
     3121 tagatcttct cgacgcgctg gtgcgacggc ctctcgacca gctccgccgg cggcgtcact
     3181 tccttgatct ctttcttctt cgccgccatg atgcccttca acgtcgcgta gcgtatttga
     3241 ctgatgcctg actgaatcgt cagcagcgca ggcagcggca tctcgtacca ctggtaccag
     3301 ccgctctcga gctctcgctt cacgcgcagt ttgtcggcag cagcgtccag ctcgatcacg
     3361 atcgtcgcgt gcggcaagcc aagcagttca gccatgatca cgcccgtttg cccgtaacca
     3421 tgatcgtccg actgcaaacc cgtcatcacg agatccggct tttcatcacg gatcgcgccg
     3481 gcgagaatag tcgccgccgc atacggcgtg aggtgcgtga gattgtcgcc aaccacatga
     3541 atcgcccgat cggcgccacg cgcgagcgca tctttgatga cgctcttcac gcgctgtggt
     3601 cccatcgagc aaacaaccac ttcaccaccg agcttctcgc gcacgcgcaa cgcttcttcg
     3661 agcgcgtagc cgtctgactc gctcacttca aacgagagat cgccctcttc aatccacgtc
     3721 gcatctttgt taatccgcag gatcgcatcc ttattcgcga cctgcttcat catcacaatg
     3781 atcttcatta accttcatac ctcttgaaca acaacgccgc gttcgtgcct ccgaacccga
     3841 agctgtttga aagcacatac tcgactcttg cttcacgagg ctcgttcgga acgtagtcga
     3901 ggtcacactc gggatcggga ttcacgtagt tgatcgtcgg tgcgatgacg ttgcggtaca
     3961 gcgtcaacac actgaacacg ctctcgatac cgcccgcagc gccgagcagg tgaccagtca
     4021 tcgatttagt cgagctgaca gcgagcttgt aagcgtgcgc gccgaacgtc tctttgatcg
     4081 cgagtgtttc gaacttgtcg ttatacggcg tcgacgtacc gtgagcattg atgtagccga
     4141 cctgttccgg cgaaactccc gcatctttta gcgttcgctg catgacgcgt cgcgcacccg
     4201 agccggtttc atccggcatc gtgagatgaa acgcatcggc cgacgcgccg tagccgacga
     4261 tctcagcata gatcctcgcg ccacgtcgtt ttgctgattc cagctcttcg aggatcatga
     4321 tcccggcgcc ctcgccaatg acaaaaccgt cgcggtctcg ctcgaatggt cgcgaagcgt
     4381 ggaccggatc gtcgttgttg gtcgacaacg ctctcatcgc cgagaaaccc gccacgctca
     4441 tcggcgtgat ggccgactca gcgccgccgc agatcatgat gtcggcatcg gcgcgttgaa
     4501 tgatcttgaa gctgtcgccg attgcgtgtg caccggcggc acaggcggtg gcggtcgcgg
     4561 agtttggtcc tttcgcgccg aaacggatcg agacctggcc cgcggcgaga ttcacgatcg
     4621 ccgagacgat aaagaatggc gaaacgcgat cgggaccatc gtcgaggagc ttcgagtgct
     4681 cgcgctcgat agcccagaaa tctccgatac cgctgctgat gtaggtgcct acgttttccg
     4741 cgatgtcggc cggcatcttg ccttcgggcg tgagctggat gccgctgtcg gcaaccgctt
     4801 cgatcgaggc cgcaatcgcg taatgaataa acgcgcccat cttgcgcgcg tctttcttgg
     4861 gaatgaatgc gagcgggtcg aagttcttga cttcgcaggc aaacctgacc gaaaacttct
     4921 cggcgtcgaa cttctcgatg tagccagcgc cactgcgccc ggccatcaga gaggcccagg
     4981 tgtcttcgac agtgttgcca accggagtga cgaggccgag gcctgtaatc acgacacgac
     5041 gtgtcaaatc agaactcctt gggaagagtt atctttagcc gcggatgaac gcggatagac
     5101 gcggatctgc atgtatccgc gttcagccgc ggctaaaatt ctactttttg tgctggtcga
     5161 tatagttgta cgcgtcgcgc acactctgaa tcttctcagc atcttcgtcg ggaatctcaa
     5221 tgccaaactc ttcttcaaaa cgcatcacga gttcgaccgt gtccagcgag tcggcgccga
     5281 ggtcgtcgat gaatctcgcg tttggcgtga cctcgttctc atcgacacca agctcatcga
     5341 cgataatctg ttttaccttg ttctcgatct cttgatcggg catcgtgttt gctcctctgt
     5401 gagttaattt aattactcaa tttgctgcag actcgctgca tcttccacgt tcagcatctt
     5461 cacgtcacga ctaatctgac gcatcagacc tgacaaaact tttccgggcc ctacttcgac
     5521 gagccgttcg acgccctgct cgatcagcaa ctgcatcgac tcgacccagc gcacgggggc
     5581 tgaaacctgt ctgacgagcg cgtcgcgcaa ctcatcgggc gctttggtcg cccgtgcatc
     5641 gacattcgtc accacgggca tggcaggtcc gctgaaattc aacgctgcga gatcaagcgc
     5701 cagcctgtcc tgtgccggct tcatcagcgc gcaatgaaac ggcgcactca ctttcaactt
     5761 gaccactttc ctcgccacac cactcaataa ctcgactgcg cgatcaacag cctcggtatt
     5821 accggcaatc accacctggt tcggcgcgtt gatattcgca atcgaacaaa cctgatcccc
     5881 gcgcgcctct gcgcaaatgc gttcgatcgc ctcgagatcg ccgccaatca ccgcggccat
     5941 cgcgccagtt ccaaccggca cggcctcctg catgtagcgt ccacgcgcac gcaccgtgcg
     6001 aacagcgtcc gtcaagctca acgcacccgc ggccacaagc gcggaatatt cacctaaact
     6061 gtgtccggcg acaaatgccg gcgcgtcaac tcccgtttcc tgcatcgccc gaaacgccgc
     6121 aacagacacc gtgagaatcg caggttgcgt gttctcagtt aactgtaaat cctctgctgg
     6181 accatcaaaa cagagccgcg aaatcgcgaa gcctaacgcg tcatcagctt cttcaaagac
     6241 ctgtcgtgct accggaaaat tctctgccaa atcttttccc atgccgggac tctgcgatcc
     6301 ctggccggga aagacaaaag ccatccgcag attacgcaga ttattctgtg tcatctgtgg
     6361 actaccattg catcaacacg gcgccccacg tcgcgccacc gccaaacgaa gccatcaaca
     6421 ccagatcacc cggcttgatc cgtcctgcac tgacacactc atcgagcgca atcggtatcg
     6481 atgccgatga cgtgttgcca tggaccgcga tgtttgaata aaccctcgac atgtcgacgt
     6541 tcaaacgatc cgcgacagct tcggtgattc gctgattcgc ctggtgcgga ataaacaagc
     6601 taatatcgtc tgacttcgca ccagcctgtt cgagcacttc ccgcgagatc tcagccatcg
     6661 atcgaacggc aaccttgaaa agctcgttgc ccctcatctt gaagtaatga tcgccggctt
     6721 cgagcgtttc tgccgtcggt ttcttgcgag tgccgccacc cggtgaaaaa agctgttcct
     6781 cgtaacgacc gtcagacttg atcttgctcg cgagaatgcc gcgaccgttt tccactggtc
     6841 ccaacactgc cgcacccgcg ccatcgccaa acaacacgca cgtctgtcga tcgctgtagt
     6901 caacgtactg cgtcaggatc tcagcaccga cgacgaccgc atgtcgcacc tggcctgttc
     6961 tgatcatcga atcggccagc gacacgccat acaagaaacc cgagcacgcg gccacgatgt
     7021 ccatcgccgc agcgttgttt gtgccaagcg ctgcctgaac gatgcagccg gttgacggca
     7081 gtatctggtc aggcgtgacg gtggcgcaaa gaagcagatc gatatcggca ggatccaaac
     7141 gagcgcgctc tatcgcctgc cgcgcagcac gcaccgcaaa catcgacgtg tattcatccg
     7201 gcgctgcctt gcgacgttgc ttaattcccg tgcgcgtcgt gatccactcg tcagaagtct
     7261 cgacaatccg ctcgagctct tcgttcgtca gaatgccttc cgggtaagag tgccccgtac
     7321 ccagaattcc agcgcgttga ttcagcatta agtcctcaga ttactacaag tatccacaga
     7381 ttacacagat gtactcagat taagtagtgg ttcactttcg ctgcaagtgt ccgataaact
     7441 ttagtttgtc gcgacattcc gacaagctaa agcttatcgg acatcagatt acgctgtata
     7501 tctctgtgta atctgtggat ggttgatccc ttcttcaatc cgctgcgcag tgcggtgttc
     7561 tgaaaactct ttcacgctcc ggatcgcatt ccggatcgca cgcgcattcg agcggccatg
     7621 tccgatcaca acgattcgac taactcccag cagcggcgcg ccgccatatt ccgtgtagtc
     7681 gagacgtttc ttgatacttc gaaacgccgg tttcgcaagc atcgcgcctg cttttgtgac
     7741 cgcagtcgac atcagctcgc gcttcaacat cgacagcatc gcctcggtca atccttctga
     7801 caacttcaac atcacgttgc cagtgaagcc gtcagtcacg atcacgtcga caagacccga
     7861 gaaaacgtcg cgaccctcga cgttgccgac gaagttgatg tcgctgagct gccgcaacag
     7921 cgggaacgcc tctttcgtga gctcgtttcc cttcgcttct tcctcgccga tgctcatcaa
     7981 accgacagaa ggcctggccg ttccgagcac cgagcgcgag taagcatcac ccatgatcgc
     8041 aaactgggcc aggtgatgcg gtttgcattc ggagtttgcg ccgacgtcga gcaacagcgt
     8101 cggtttcgcg gctttcgtgg gaatcatcgc ggcgagtgcg ggtctatcga cacccggcaa
     8161 cgttccgatc accatcttcg cagttgccat cgccgcaccg gtgttgcccg cggagacgaa
     8221 tccgtcaccg cgaccatcca tcacgagctt cgaaccgatc ctcaaagagc ttttcttctt
     8281 ccgcctgacg gaagtggcga ctggatcatc catcgcgatg acttcagccg cgtcaaccag
     8341 ctcgatcccg cggtcgccgt cgccgcgcca accgcatctt cgcaactcgg gctcgacctt
     8401 gtgggcctgg cccaccagaa tcacgccgac gccgaagtcg cgcgtcgccg caagcgcgcc
     8461 atcaatctct acgttgggcg cgtggtcact tcccatagcg tcgagaacga ccttacccgt
     8521 caactggggc tgatgctgcg actgacttcg cacaacgtcg actagctttg ttctacgtgt
     8581 accacttcgc gacctttata aaagccgcat ttggggcaaa cgcggtgtgg catgcggggc
     8641 tcacggcaat tcggacacag tgtggtttgg gggacgcgca aagcatcgtg cgcgcgtcgt
     8701 ttggcggtgc gagctttaga gtgtcgtcgt ttaggatttg gcataattac tccattaaat
     8761 tcgccacaaa gaggcacaaa aagcacaagc ttgattctta actgttttgt gctttttgtg
     8821 cctttttgtg gcctaagtca agttcttcgt caaattcttc agagccgccc aacgcggatc
     8881 gatatcattc gtaacgcaat tgcactcgcc cgtgtttctg tcggtgccgc attccgggca
     8941 gatgcctttg caatcctcgc ggcaaagcat gcgagttggc acggccaata aaatctgttc
     9001 cttcacgatc tcgtcaacat cgatcgcggc gccgtcgaac acggaaacca acatctcggc
     9061 ttcggtcagc tcagccacct gactcgattc gtagtccgag ccgctcatgt attccagcgt
     9121 gaagtcggcg ttaaccggca gctcgactgg tttcaaacag cggtcacatt cgacctgggc
     9181 tcgcgtttcg acgtgcccgt tgacaaagac ctcgttaccg gaaagccgga ctttgccgtt
     9241 aacggctgcc ggcgcaatca aactcactcg atcgtcgact gggttcagtt catcaggctg
     9301 gtaaacgtga gcgaaatcgc ccttactacc ctccaggttc tccagttcaa tacgcatacc
     9361 gcacaaaaag cggccattat aggcgattag tgtccgttcg taaaatcagc cgacaaagcg
     9421 tcttctcgca acgcctctgc gagacgctta tccgccccgg tcatcaaccg catggccccg
     9481 gatttcgtca cttgaaacag gcttccattt ttgcgtccgg ctttttctaa agcttgccgt
     9541 gccagttctt tacgtcccgt tcgttcataa gcttgtgcaa gtaatgagaa cgcctcagcg
     9601 tagttgccgt ccatctcggt cgccttgcgt aagtgccgaa cggcgagcgt gtcgcgattt
     9661 agctcgtagt aaagacagcc cagaacgtaa tgtgcttctg ctgacggtcg caccgtcaag
     9721 gctcgcttga actcaccgag cgcacgccgc caattctttt cggccacaaa gacccagcca
     9781 aggccgcagt gtcccgcgaa cgaggaacag ccctgcgcag tcgcactact caaaagatcc
     9841 ttcgcgagct cgacctcgcc gtcgtcagca cacgtcaatc cgagtaacaa agagatgcgc
     9901 agatcgtccg gcatcgcggc gtgtgccttc gcgagataag cgcgtgcctg ctttgtctta
     9961 ccgcggcgga agtaatgctc accgataaac agattcaacg gtgcgttctc tgccgcgatc
    10021 tcagccgctg cctgaagctt gctcatccct tgcgggacct gccgatcttc gatcagcaca
    10081 aaaccttcgt tgacgagccc ttcgaacact tgtttgtctg gcacctgtgc agttgcgagg
    10141 atgcgcaaac gcaacgcatc gcggtgcagg ttctcttcct gttcgactgt gtcgcgctgg
    10201 cagcgttcgc tccacattcg ttcgagtcgc tctgtctcga tcacgccgtc ttcagttaag
    10261 agaccaacca gggctctcat cagcgagcga tcgaagtaag cgttctgtgc gtagcgcagc
    10321 agtaagtcga gactgcgctc gaggcgttcg gccaatcgtg cgactctgtt ttcagccgct
    10381 gagatacgtt cgagcaggtg ttcgtcagtt gagagacttg taggcgcggt ttcgaaacgc
    10441 gtggagctcg gctcgacgac gatcatcaag cgcgtgccgc agcgagtgca gaactcctgg
    10501 cccagcgcgt ttgccgcaag acatttttga cagtagtgtg agatgccggg gcgcattcgg
    10561 ctacctttaa tattcgtaaa acccacgacc gctttttcta ccgagccagc cggcgtcgac
    10621 atagcgcttc agcagcgggc acggcctgta tttggggtct ccgagcccct catataacac
    10681 attcatgatg gcgaggcata catcgagacc gataaagtcg gcgagcgtga gcgggcccat
    10741 cggatggttc atgccgagct tcatgatgcc gtcgatgctc tcgcgactcg ctacgccttc
    10801 gtaaagagta aagatcgctt cgttgatcat cggcatcaac acgcggttcg agacgaagcc
    10861 gggcgagtcc tggcaatcga gcgccgtctt gcccagctgt tcggagagtg ttctcacgcg
    10921 ttcgtaagtt tcgtcggatg tcgccatgcc gcggatgact tcgatgagct tcatcaccgg
    10981 cacgggattc atgaaatgca taccgatgac tttgtccgga cgtttcgtgg ccgcgcccag
    11041 tttcgtgatc gagattgaag aagtgtttga agcgaggatc gcgtgtggcg gcgtgacttc
    11101 atcgagcatc ttgaagacct gcgtcttcac gcccagatcc tcgaccactg cttcgacaac
    11161 gaacgacgct tctttgaacg ctgcgaaatc agtcgtcgtc ctgattttct cgataacggc
    11221 tttctttccc gcgccgtcga gtcgctcttt gtcgacgtcg cgttgtaggc tcttatcaat
    11281 cgcctttagt ccccgatcga gaaactcgtc acgaacgtcg tgcatgatca cgttgtaacc
    11341 agctcgcgcg gagacttgag cgatcccatt ccccatcgtg ccggcgccaa caacgccaat
    11401 agtgacagga ttggcaggat tttcagaatt tgcaggattc atcttgttca ttgtgtcaat
    11461 cttggaaatc ctgtctaacg ctcaatcgcc atcgcaacgg cgttgccacc accgagacac
    11521 aacgccacaa ttcctcgtcg tgcatcgcgt ctcttcagct catataacat cgtcgtcaac
    11581 aatctcgatc ccgattgtcc gatcgcatgg cccaatgcta ccgcgccgcc gttaacgttc
    11641 accttctccg gatccagccc gagctcgcgc gtgactgcca gcgcagccac cgagaacgct
    11701 tcattcaact ccaccagatc gacttcacta ccggaccaac cagtcttctt caacaatttc
    11761 cggatcgctt caaccggcgc catcatcacg aggcgcggct caacacccga cacagcctgc
    11821 gccacaatct ttgccatcgg ctcgatacca agctcacgcg cacggcgcag cgaagtaacc
    11881 accaccgctg acgcaccatc attaacactc ggcgcattac ctgccgtcac tgttccttca
    11941 ctcttgaacg ccggcttgag ttttccgagc acttcaaccg tcgtatcttc acgcacagtc
    12001 tcatcagtat cgaaaatgag cgccgcgcct ttcttctgct tgatctcgac cggcacgatc
    12061 tcgtctttaa acttcccggc cttgatcgcc gccgctgctt tgcgatgcga gctcgtcgca
    12121 aactcgtcct gctctgtgcg cccgatcttg tactcgtccg ctacaacttc acccgtgcag
    12181 cccatgtgat aattctcaaa tgcgcaccac agtccgtcat gaatcatcga atcgattaac
    12241 tcgccgttcc cgagccggta accctcacgc gctttcggaa tgagataagg cgcgttgctc
    12301 atcgactcca ttccaccggc aacaactatc tcggagtcgc cgagttgaac tccctgcgcg
    12361 gccatcatca cggccttcaa accagagcca caaaccttgt tcacagtcac ggcagcaacc
    12421 gtcgaaggta tcccaccatt caacgccgcc tgtcgcgcgg gattctgacc gagtcccgcc
    12481 tgaatcacgc atcccatgat cacttcgtca acatcttccg gcttgattcc cgcgcgcttc
    12541 acgctctcgc gcaccacgat cgcgcccaac tcaggcgcgg tgaatccttt caacgaaccg
    12601 agaaactttc ccaccggcgt gcgcgccgca ctgataatta ctgcttcgtt caattccggc
    12661 atttcaaact cccttatgtt agacttggcc cgatattaga ctttatttca ttattcataa
    12721 agcatgcagg cactcattct tgctggcggc aaaggcacgc gccttcgccc tttaacagtt
    12781 tacactccga aaccgattgt tccgatctgt aaccggcctt ttcttcttta tcaaatcgac
    12841 acgctgcgcc gcgcgggcat caccgacatc acgctctcgc tctcttatca accaaacaaa
    12901 atcgagcaac tgctcggcga tggctccgat ttcggcgtca agctgaagta cacagtcgag
    12961 ccgcaaccga tgggaaccgc cggcgcttac aagtttgccg aagatctcat ccgcgagcca
    13021 acggtcgtgt tcaacggcga cattcttacc gatctcgatc tcaaggccgt cattcgcgag
    13081 cacaccgagc gtaaagcgac ggcgacgatc gtcctcgcac cggttgagaa tccacaaagt
    13141 tatggcctcg tcgaaacgga gaaagacggg cgcattcgtc gcttcctgga aaagccgaaa
    13201 gctgacgaga tcacgtgtaa caccattaac gccggtacct acattctcga gccgaagatg
    13261 ctcgactatt tgccggctgg tgagaattac tcgttcgagt atggattgtt cccggatctt
    13321 ctgaagcgtg gtgaagcgtt ttacgcgcac atcccggtgc gcacgtactg gatcgacatc
    13381 ggcacaccgg agcgatatct acaagtgcac gatgatatgc ttgcgaatcg tgtttcgcgg
    13441 tttcatatca aggagcgacg cgggcagttt gacgcagcga cagtcgcgga aattgatgag
    13501 ttgtcgatga tcggtgacga ctgccagatc aagccgggcg cgcagatcat caactcggtg
    13561 ttgggccagg gatgctttgt cgaggagcgt gcgcgggttg agaactcagt catctggccg
    13621 cacacacgca tcggcacgac cgcgcaagtg agcggcacca ttgtcgggcg tggttgtcac
    13681 atcggccgct cggccatcgt tagacccggg acagtgctgg gtgataaaac ttcgctgacc
    13741 gactacacgt tcgcataagt ttaaatccat cacagattac acagaatctg cgggatggtt
    13801 cgaatcagag ggaatcatga actgggacgt ttttcgagca tacgacattc gaggcaccta
    13861 ccccgatcag atcaatgaag aggattacta ccgtaccgcc aaagcatatg tttacctctt
    13921 caaaccaaaa acaatggtcg tcggcatgga tgctcgtgtt tcctccccgg ttctcaaagc
    13981 gggtctgacg cgcggttttc tcgacgcggg tgttgacgtc gtagacatcg gacagatcac
    14041 gaccgacatg ctctattaca ccgtcggcgc acattctgaa tattcaggtg gcatcgtcgt
    14101 ctccgcctcg cacaatccgg tgcaatacaa cggcatgaag atggtccgcg agaaagcgac
    14161 cgctatctct tctgacaccg gcctcttcga catacgcgac accctcaaag ccggtaaaga
    14221 tgcggaagtc acttccgaca aaaaaggcac ttgcaccgag cgcgacatac tcaacgatta
    14281 tctcgaacac gtgctgaagt ctatcgatcg aacaaagatc aagccgttca agttcgtcgg
    14341 caacgccaac ttcggatatg tgtgccggcc ggctgcaaag ctcgccaaag atttgaatct
    14401 gacccttctc ccgctgaact tcgaacccga tggcacgttt ccaaagggac caccggatcc
    14461 gatgctgccg ggcaatcgca ccgaaacaga aagcctgatc aaagaaaagg gcgcgtcgtt
    14521 tggtgtggcg tgggatgctg atgccgaccg cgcgatgttc ttcactgaga aaggtgagtt
    14581 catcaacggc gcgtacatca ctgcgtttct ggccgatatc cttttgacga agtacggaag
    14641 tgataacacg atcatcttcg atccacgtgt gatatggccc acgctcgacg ccgtgaagcg
    14701 acacggcgga cggccgatca tctcaaaggg cggccacgcg tttatgaagg atcgcatgcg
    14761 gaaggagaac ggcctgtttg cgggtgagat gagcgggcat tattactttc gtgaaaattt
    14821 ctacgccgac aacggagtga ttcctttcct gctcgtgctc gaacatctct cgacacacga
    14881 taaaccgttc tcagagctga ttgcgccgtt catggcgggg cactacatgt caggcgagct
    14941 caattaccac gtcaaagaca tcaaagccgt gatcgagaag gtgcaggaga agtttcacgg
    15001 cgaggggacg gaagatttta ctgatggcta ctcgctcgag accgccgagt ggcgcttcaa
    15061 tatcagaggc tcgaacacgg aaccactctt gaggttgaac atcgaggccc gcaaggatgg
    15121 attggttgat cacatccggc aggagattga gaagttaatc gagtcgtagg cttcatccac
    15181 agattacaca gattgttata aaaatctgcg taatctgtgg ataagtttct ttaacatcaa
    15241 tgcctgagct tcctgaagta gaacacgtcg tccgcgcttt atctccggtc gtcaccggac
    15301 ggcgcattct cgctgcggaa ctgaatctta aacgcatcgc accgggcatc tcgcgtcctg
    15361 cattcaatcg ccaactacaa aacgcgctca tcacgggtgt cggccgtcgc ggcaagtaca
    15421 tccttttcaa actcgagaca ggacacgtac tcaccacaca tctgcgcatg accggcaagt
    15481 ttgtctcact cacgatcgac gaatcactgc cgccgtacgc acacgtcgtc tttcatctcg
    15541 acgacgatcg acgtctcgtg tactgtgata tgcgccagtt tggacgcatg cagatcttca
    15601 aagatccatt gcgtccaccg aaagagctgc aaacgctcgc gcctgagcca ctatcagacg
    15661 aattcacgga agagtatttc ctcaacactc tcaaacgctc cggtcgtccc ttgaaacatt
    15721 tgttattgga ccagacacgc gtgctcggtc tcggcaacat ctacgccgtt gaagcgctct
    15781 ttctcgcggg cgtcagtccg atgaaggccg caaataagct ctcaaagcca cgcgcgcgca
    15841 aactgtacca ggcgattcgc gatgtactac aggaagccat cgacgcgggc tcgaccttga
    15901 agatcgatct cgccgatgga aacagcagct acgtcgggac ttcggagcgt ttctggcgtg
    15961 tctacgaacg cgaaggcgaa ccatgtgtga gatgtggaac taagataaaa agaatcgtcc
    16021 agggcggaag atccacttac tactgtccca aatgtcagaa gaactagggc aaatcgccgc
    16081 actcagtaac aattaatgtt acctatcgcc taacagtttg cggcagtttg ctacaccggg
    16141 ccgcaagact tactgctctt cttctttacc ggaataagga aagtcgtccg ccaga
//
LOCUS       DASSFQ010000147         4575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1254965, whole genome shotgun sequence.
ACCESSION   DASSFQ010000147 DASSFQ010000000
VERSION     DASSFQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254965"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFH96_05190"
     CDS             complement(<1..735)
                     /locus_tag="VFH96_05190"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973396.1"
                     /translation="MQQRIPKILIISSDTGGGHHSAAAAIVAGVQKFFEGESYAIRVV
                     RAVEESHHLSAKLVRLYNWILRNRQHWMKYFYWCMNHIRPETSRFFYKRCVGYVVELF
                     ERWCPHVVVSVHPLTQHIFARVLKELNLAGRVPLVSVVTDPCYGFWKGWACDDVTLYL
                     VASDDARRQLIDYGVAPERIKISGLPVHPKFAYPGEEAAQAARRALGLDPEKFTVFVN
                     AGWIGGGNIPQIFSELVRGELDVQAIF"
     gene            complement(809..1972)
                     /locus_tag="VFH96_05195"
     CDS             complement(809..1972)
                     /locus_tag="VFH96_05195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6973397.1"
                     /translation="MDFTTKELLKRYAKLAIGQPFSPVLLNILVTSVCDMRCTHCFFT
                     DELDDRPRKKLQMKTHEIERISQTLGGNLGVLILAGGEPFTRKDLPEIARAFYTNNKV
                     ESIYIMSNGQIQKRIFPDVTRVLQECPNLNVTIALGIDGPKLEHEKIRQKPGSWDIAI
                     DTARQLQAIKKEFPRLDVQTCTCFMNSNQDTIFEWYDFLKYDLKPDKVNFNYIRPPSA
                     DPKELDIDHTRYAKLAQMIDDDSRHAAIKNNYSGDAGFFKAAIDIYMHGLIAKTQETQ
                     TAQMTCYAGTAGGVIYDEGTVSSCENLDPVGNLRDFDWNFQKLWLSPAMQARRKKAHD
                     GCFCTHESNSYYPSLPFNPGHLIQIKKLEREMKKARKAVEKAEHAGVPVVDLN"
     gene            complement(2050..3555)
                     /locus_tag="VFH96_05200"
     CDS             complement(2050..3555)
                     /locus_tag="VFH96_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HET6973398.1"
                     /translation="MLCVLASFGFGFGVSFVSRRATLPPEARIQIADFRVAIPEGETV
                     VTITSRDASNEELGKLTIQRYGNTISGVPEVQRRIEPRHELTTEDAAYLRRELSSEIS
                     LSESPWQRANKIRLWLAQRCYRVGMPGLATREPRKAYDEMKGGKPVLCGNLAEIYVAL
                     CQAMGLTARAVGLSVAVQNGLFGIDTHAAAEVWLPEMGGWIYQDPTFNCYWKIDGKPA
                     SAMLLHDALMEKLPIEFAPQDQQTEDRLENYYLDPRLYFRHISYEYKAGGTVLYFADP
                     RLEPLNLRDKNWIYTDNRVDIQRLDAGGNFVIERRSEISPGVFVQVVGNDLFVRDRRE
                     QSPGIRVRSSSGMVEGCAYLHQRAQDMGLFNGANLARNPSFRLSSSNQLADEWSVAGP
                     VEAMTVSGGQAMSALAGGRLWQRIQVRRNGRYLLYARVSVSRGFVNWSLADAERGPRS
                     VGTIEPERISEVVSDVVESQSGYLDVGFDVPSGGAFRVIDVIVTEAPRFVQ"
     gene            complement(3802..>4575)
                     /locus_tag="VFH96_05205"
     CDS             complement(3802..>4575)
                     /locus_tag="VFH96_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6973399.1"
                     /translation="FIEVNSAASPEELIESELFGHVKGAFTGATAAKKGKFELADGAT
                     IFLDEVGDMSPKVQAKVLRVLEEQRFEPVGSGTSITVDVRLIAATNKRLDVEIDQGNF
                     RADLFYRLNVIPFELPPLRDRLEDVPLLVDYFNQHFSKAYGKRPKQFLTEAVEAMQAY
                     LWPGNVRELRNTIERVVIMHPGTKVSANDLPPQGKEEPPAASFRFPSFKEATEAYHRA
                     FIQRKLEEADGNVSRAAELMGVDRSHLYRRMRALGIQKT"
BASE COUNT          985 a         1303 c         1254 g         1033 t
ORIGIN      
        1 gaagatcgcc tgcacgtcga gctcgccgcg caccagctcg ctgaaaatct gcggaatgtt
       61 gccgccgcca atccaacccg cgttcacaaa caccgtaaac ttttccggat cgagccccag
      121 cgcgcggcgt gcagcctggg ccgcttcttc gccgggatat gcaaacttcg gatgcaccgg
      181 cagacccgaa atcttgatcc gctccggggc cacgccgtag tcgattagct gtcgacgcgc
      241 atcgtcactc gcgacgaggt agagcgtcac gtcgtcacaa gcccagcctt tccaaaagcc
      301 gtaacacgga tccgtgacca cggagacgag gggaacacgt ccggcaaggt tcaactcctt
      361 caacacgcgc gcgaagatgt gctgcgtcag tggatgcaca ctgacgacaa cgtgcggaca
      421 ccagcgctca aacagctcga cgacgtaccc gacacagcgt ttataaaaaa acctgctcgt
      481 ctccggccga atatgattca tgcaccagta gaaatacttc atccagtgct gtcggttgcg
      541 caggatccag ttatacaggc gcaccagctt cgcgctcaga tgatgcgact cctcgacagc
      601 gcgcacgacc cggatcgcat acgactcacc ttcgaaaaac ttctgcacac ccgcgacgat
      661 cgcagccgcc gcagagtgat gcccgccacc agtgtcagac gagatgatca ggatcttcgg
      721 gatgcgttgc tgcattttat gtttgctgtt ttatccacag attgcacaga ttcattaacc
      781 aatctgcgta atctgtgggt aagaattgct aattcaaatc cactaccggg actcccgcgt
      841 gttctgcctt ctccaccgcc ttgcgcgcct tcttcatctc gcgctccagt ttcttgatct
      901 ggatcaagtg tcccggattg aacggcaacg acgggtaata actattcgac tcatgcgtgc
      961 agaagcagcc gtcgtgcgct ttcttgcgtc gtgcctgcat cgcgggtgac agccagagct
     1021 tctgaaagtt ccagtcgaag tcgcgcaggt taccaaccgg atcgaggttc tcgcaggacg
     1081 agactgtacc ttcgtcgtaa atcacgccgc cggcagttcc ggcataacac gtcatctgcg
     1141 cggtctgagt ttcctgcgtc ttcgcgatga gcccgtgcat gtagatatcg atcgcggctt
     1201 tgaagaaacc ggcgtcgccg ctgtagttgt tcttgatcgc ggcgtggcgg gagtcgtcgt
     1261 cgatcatctg cgccagcttt gcgtacctgg tgtgatcgat gtcgagctcc ttcggatcgg
     1321 ctgaaggtgg acggatgtaa ttgaagttca ccttgtcagg cttgagatcg tatttgagga
     1381 agtcgtacca ctcgaagatc gtgtcctggt tcgagttcat gaagcaggtg cacgtttgca
     1441 cgtcgagacg cggaaactcc tttttgattg cctgtagctg gcgggcggta tcgatcgcga
     1501 tgtcccacga gccgggcttc tggcgaatct tttcgtgctc gagcttcggt ccatcgatac
     1561 cgagcgcgat cgtgacgttc agattcggac actcctgcag gacgcgcgtg acgtccggga
     1621 agattcgttt ttgaatctgc ccgtttgaca tgatgtagat cgactcgact ttgttgttgg
     1681 tatagaaagc gcgggcgatt tcgggaaggt ctttgcgcgt aaacggctcg ccgcccgcga
     1741 gaatcagcac accgagattg ccaccgagtg tctgcgagat ccgctcgatc tcgtgagtct
     1801 tcatctggag cttcttgcgc ggccgatcgt cgagctcgtc tgtaaaaaaa cagtgcgtgc
     1861 agcgcatatc gcaaaccgac gtcaccagaa tgttcagcaa cactggtgaa aacggctgcc
     1921 caatcgccag cttcgcataa cgcttaagta attctttagt cgtaaaatcc atcgctacct
     1981 caatgatgtt gataagcgat ttagagatgt attgtcaatt ggttaattgt tgaaaggact
     2041 acaggatctt cactgcacga agcgtggggc ttcggtgacg atgacgtcga tgacgcggaa
     2101 ggcgccgccg gaaggaacgt cgaagccgac gtcgagatag ccgctctgac tctcgacgac
     2161 gtcagaaacc acttcactga tccgctcggg ctcgatcgtg cccaccgatc gggggccgcg
     2221 ttcggcatca gccagtgacc agttcacaaa gccacgcgag acactcacgc gcgcgtacag
     2281 caaatatctt ccgttccgcc gcacttgaat gcgttgccac aatctcccgc cggccagcgc
     2341 tgacatcgct tgcccgccgg acacagtcat tgcttcaacc ggtccggcta ctgaccactc
     2401 atcggcgagt tgattcgaac tgctcagacg aaaggatgga ttgcgcgcga ggttggctcc
     2461 gttgaataac cccatatcct gtgcgcgctg gtgcagatac gcgcagccct cgaccatgcc
     2521 actcgacgag cgtacgcgaa tgccggggct ctgctcgcgc cggtctctca cgaacagatc
     2581 atttcctacg acctgcacga aaactcccgg cgagatctcc gagcgtcgct cgatcacaaa
     2641 attgccaccg gcatcgagac gttggatgtc gacgcgattg tcggtgtaaa tccagttctt
     2701 gtcgcgcagg tttagcggtt cgagtcgcgg gtcggcgaaa tagagcacgg tgccgcctgc
     2761 tttgtattcg taggagatgt ggcggaaata aagacgcgga tcgagatagt agttttccag
     2821 tctgtcttcg gtctgttggt cctgtggtgc gaattcgatc gggagctttt ccattagtgc
     2881 atcgtgaagc agcatcgcac tcgcaggctt gccgtcgatc ttccaataac agttgaaggt
     2941 tggatcctga taaatccagc cgcccatttc cggaagccag acttccgcag cagcatgagt
     3001 gtcgatgcca aacagtccat tctgcaccgc cacgctcaag ccgaccgcac gtgccgtcaa
     3061 tcccattgcc tgacaaagag cgacataaat ctcggcgaga ttaccgcaca ggacgggctt
     3121 tccgcctttc atctcgtcgt acgctttgcg cggctcgcgc gtagccagac ccggcatgcc
     3181 gacgcggtaa cagcgttggg ccagccacag gcgaatcttg ttggcgcgct gccagggtga
     3241 ttcggaaagt gaaatctcgc tgctgagctc gcgacgcagg taggcagcgt cttcggtagt
     3301 aagctcgtgg cgtggctcga ttcgtctttg aacttcgggc acaccggaga tcgtgttgcc
     3361 gtaacgttga atggtgagtt ttcccaactc ctcgttactg gcgtcacgac tcgtgatggt
     3421 gacgaccgtt tcgccttcag gtatggcgac gcgaaagtca gcgatctgaa ttcttgcttc
     3481 tggcggaagt gttgctcggc gcgagacgaa tgaaactccg aagccaaacc cgaacgacgc
     3541 gagaacacac agcaacaggg gccacttaga tctcatattg aggaacgttt acaagactga
     3601 atgcggactg aaccctgacg gacgcgtcgc caaaatacat ggtctatgta gcgagcacgt
     3661 catgcttgtg taaagttttg tcaattacct ataagaattt ttagccacaa aaggcacaaa
     3721 aagcacaaaa gaaagagttc agagtgctag agctgggatt ttcccttttg tgctttttgt
     3781 gccttttgtg gctaaaaatt cttatgtttt ctggatgccg agggcgcgca tgcggcgata
     3841 gaggtggctg cgatcgacgc ccatcaactc cgcggcgcgt gagacgttgc cgtcagcttc
     3901 ttcgagcttg cgctggataa atgcacgatg gtaggcctcg gtcgcttctt taaaggacgg
     3961 gaatcggaac gacgcagccg gcggctcttc tttgccttgc ggcggtaaat cgttggcgga
     4021 gactttcgtg cccggatgca taatcacgac gcgctcgatc gtgttgcgca gctcgcgcac
     4081 gttgccgggc cacaagtacg cctgcattgc ctcgacagct tccgtcaaaa actgtttcgg
     4141 tctcttgcca taagccttcg agaaatgttg gttgaagtaa tcgaccagca gcggcacgtc
     4201 ttccagccgg tcgcgcaatg gcggcaactc aaacgggatc acattcaaac gatagaaaag
     4261 atccgcacga aagtttccct gatcgatctc gacgtcgagt cgtttgtttg tcgcggcgat
     4321 caaacgaacg tcaaccgtga tcgacgttcc actgccgacg ggctcgaagc gctgctcttc
     4381 cagcacgcgc aggaccttcg cctggacctt cggactcatg tcgccgactt cgtcgagaaa
     4441 gatcgtggca ccgtcagcga gctcaaactt gcctttcttc gcggcggtgg cgcccgtaaa
     4501 cgcacccttc acatgcccga acaactccga ttcgatcagc tcttccggac tcgccgcgga
     4561 gttgacttcg atgaa
//
LOCUS       DASSFQ010000148         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2147658, whole genome shotgun sequence.
ACCESSION   DASSFQ010000148 DASSFQ010000000
VERSION     DASSFQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2147658"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFH96_05210"
     CDS             complement(<1..573)
                     /locus_tag="VFH96_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973400.1"
                     /translation="MDSILIVDDERGIRDTLRAVMEDEGFVADAVATGEECLKALGRR
                     AYGCILLDVWLPGIDGLETLKQLREAGSDAAVVIISGHGNIETAVRATKLGAFDFIEK
                     PLSIEKTVLTVRNALRQQKLELINADMAAALAKEYTMVGGSVAMRALRKQISVVAPTD
                     GRVLIYGESGTGKELVAHAIHAESRRAAAPF"
     gene            complement(642..2873)
                     /locus_tag="VFH96_05215"
     CDS             complement(642..2873)
                     /locus_tag="VFH96_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973401.1"
                     /translation="MVDNRENRIRRRRRILLWVLGATVLLLLAIIVSQQLWLWTVVRP
                     DTPADALVLYALSTLNFVAFVVFLFIFVRNLLKLRRERRERQLGSKVKTRLLVYFISI
                     SFLPITAMAFFSYLFLNRSLEKWFNPFPDEIVQQATEVKRLTLSSVDQTLRDTAALLV
                     MLLNGQTPEERLATVNQIVNSGQLVGIEIVRTDGLVETQSRAPLSASDAEQLDKLFGE
                     ARKFRGPSNSSLVDGKEFDALAVPLNQSEMLIIAPQKRASNDLTDLVTDSERGYQNLV
                     GQQRRVRLLGLSTLGLMTLILLFVSSWVAIYLARGIATPIKALAEASNEVARGNLAHR
                     VTTTIADDELALLAESFNQMTAQLQENRSRIEAGASELRDTNMALRERRNYIETVLQS
                     LSTGVVSIDENDCVTTLNASAARMLALGEKANDDPKLSGLIGSEDWLVVERLLRRARR
                     TGHATEQTQLESGGANGNGPLPVSLTATALRGSKGPRRGVVLVIEDLSELLAAQRAAA
                     WSEVARRMAHEIKNPLTPIQLSAERIAKSYGRATAPSNGANGAGEPYDDKRVAAVISE
                     CTETITREVAGLKAMVDEFSRFARLPATRLDLANLNDVVTQAAGLYVDRLDGVVLTLD
                     LDRELPMSMLDVEQIKRVFVNLIDNAVQALASANDEMQITITTSHDAERSLLRAEVAD
                     TGHGIEPGDFRRLFEPYFSRRDSGTGLGLAIVQRIIMEHGGHIRAERNHPRGAKFVIE
                     LPV"
     gene            3118..>3422
                     /locus_tag="VFH96_05220"
     CDS             3118..>3422
                     /locus_tag="VFH96_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973402.1"
                     /translation="MITTALRIGVIFVLGRTTFFIDNLTFRAGLLASALGAGYFCVRF
                     LEGLPWRSLGLTFHAGWLRDLIFGFVIGFASLVVAVAIASKGFTFSVGHNGWFWIVRS
                     "
BASE COUNT          708 a         1004 c          948 g          762 t
ORIGIN      
        1 gaacggcgcg gccgcgcgac gcgactcagc gtggatcgcg tgtgcgacga gctcttttcc
       61 agttcccgat tcaccgtaga tcaggacgcg accatcagtc ggtgccacga cggagatctg
      121 tttgcgcaac gcgcgcatcg cgactgaccc gccgaccatc gtgtactcct tcgcgagtgc
      181 ggcggccatg tcagcgttga tcagctcgag tttttgttgg cgcagggcgt tgcggaccgt
      241 gaggacggtt ttctcgatcg agagtggttt ctcgatgaag tcgaaggcgc cgagcttcgt
      301 ggcgcgcacg gcggtttcaa tgttgccatg accggagatg atcacgactg ctgcgtctga
      361 gcccgcttcg cgcagttgct tcagagtctc gagaccgtcg atgccgggaa gccagacgtc
      421 gagcaaaatg cagccgtacg cgcggcgacc gagcgctttc aaacattctt caccggtggc
      481 gacggcgtca gcaacgaagc cctcgtcttc catcacggcg cgcagcgtgt cgcgaatacc
      541 gcgttcgtcg tcaacgatga gaattgagtc cattacagtt ttttatccac agattacaca
      601 gatttgcatt tacgtctgcg taatctgtgg atggctctgg tctacactgg caattcaatc
      661 acaaacttcg cgcctcgcgg atggtttcgc tcggcgcgga tgtggccgcc gtgctccatt
      721 atgattcgtt gcacgatcgc gaggccgaga ccggtgccgc tgtcgcgtcg cgaaaaatac
      781 ggctcgaaca gacggcggaa atcgcccggc tcgattccgt gtcctgtgtc ggcaacctct
      841 gcgcgcaaca gactccgctc cgcatcgtgg ctcgtcgtga tcgtgatctg catttcatca
      901 tttgcggacg cgagcgcctg caccgcgtta tcaatcaggt tcacaaacac gcgtttgatc
      961 tgttccacgt cgagcatcga catgggcagc tcgcgatcga gatcgagtgt taacaccacg
     1021 ccatcgagac gatcgacata caaacctgcc gcctgagtca cgacgtcatt taagtttgcg
     1081 agatcgagtc gcgtcgcagg cagacgagcg aaacgtgaaa actcatcgac catcgccttc
     1141 aaacccgcca cttcgcgcgt gatcgtctcg gtacattccg agatcactgc cgcaacgcgc
     1201 ttgtcgtcat aaggttcacc agcgccatta gcaccattac tgggggccgt cgcacgcccg
     1261 taactcttcg cgatccgttc ggcggaaagc tggatcggcg tcaaggggtt tttgatctcg
     1321 tgcgccatac gtcgcgccac ttcgctccac gcggccgctc tctgcgctgc cagcaattcg
     1381 gaaagatcct cgatcaccag cacgactcca cgtcgcggac ctttcgaacc gcgcaaagcc
     1441 gtggccgtca acgagactgg cagtggacca ttaccattcg caccaccgct ctccaactgt
     1501 gtctgctccg tcgcgtgacc agtacggcgc gcgcgacgca gcaatcgctc gacgaccagc
     1561 caatcttcac tgccgatgag accactgagc ttcggatcgt cgttagcttt ctctcccaac
     1621 gcgagcatcc gcgccgccga cgcattcaat gtagtaacac aatcattttc gtctatcgag
     1681 acgacgccgg tcgataacga ctgaagtacc gtctcgatat agttgcgtcg ctcacgcaac
     1741 gccatgttcg tgtcgcgcaa ctcggacgcg cccgcctcga tgcgactacg gttctcctgg
     1801 agttgcgccg tcatctgatt gaacgactca gccagcagcg ccagctcgtc atcagcgatc
     1861 gtcgtcgtga cgcgatgcgc gagattgccg cgcgcgactt cgttcgacgc ttcggcaagt
     1921 gccttgatcg gcgtcgcgat tccacgcgcg agatagatcg ctacccagct cgaaacaaac
     1981 aacaggatca gcgtcatcaa tccgagcgtc gatagaccga gcagacgcac gcgccgttgt
     2041 tgccccacga gattctggta gccgcgctct gaatccgtta cgagatccgt cagatcattc
     2101 gacgcgcgtt tttgcggggc gatgatcagc atctccgatt ggttcaaagg cacagcgagc
     2161 gcgtcgaatt cctttccatc aacgagactg ctgttgcttg gaccacgaaa cttgcgcgct
     2221 tcgccgaaga gcttgtcgag ttgttctgcg tctgaagcag ataacggagc gcggctctgc
     2281 gtctcgacca aaccatcagt cctgacgatc tcgattccga ctaactgtcc ggaattgacg
     2341 atctggttca ccgttgcgag gcgttcttca ggtgtttgcc cgttcagcaa catcacgagc
     2401 agcgcagcgg tgtcgcgcag tgtctgatcc acgctcgata acgtcaggcg cttgacttca
     2461 gttgcctgct gcacgatctc gtccggaaaa ggattgaacc acttctcgag cgagcgattc
     2521 agaaagaggt acgagaagaa cgccatcgcc gtaatcggca gaaagctgat cgaaatgaag
     2581 tagaccagca gacgtgtttt aactttcgat ccgagctgac gttccctgcg ctcgcgacgc
     2641 agcttcagca gattgcggac gaagataaag agaaagacga caaacgcgac gaagttgagt
     2701 gttgataaag cgtagaggac aagcgcgtcc gcgggtgtgt cgggtcgaac gaccgtccac
     2761 agccagagtt gctgcgacac gataattgcg agtagcaaca gcaccgtcgc acccagcacc
     2821 cacagcaaga tgcggcgccg tcggcgaatg cggttttctc gattatcgac cattttattc
     2881 ggagtttaac ataggcgaga tcttctctgc ggtcctctgc gtatctctgc gccctctgcg
     2941 tttgaggttg acgtttaacg cagagaacgc agagatacgc cgaggacaca gagaaaatcg
     3001 atcaatcctg atagaatgca gcgcccgatg cgcgaaatct tcattaatga atttggacgg
     3061 ctccggagtg ggtggcgttt acaaatattt gccgctgcgt ttatcgcgct ctcatttgtg
     3121 ataacgactg cgctgcgtat tggcgtgatc tttgtgttag ggcgcacaac atttttcatc
     3181 gacaacctga ctttccgagc aggactctta gcttcagcac tcggtgccgg atacttttgc
     3241 gtgcggttcc ttgaagggct accatggcgc tcgcttggtt tgacgtttca cgccggatgg
     3301 ctgcgcgatc tgattttcgg atttgtgatc ggctttgcga gcctcgtcgt ggcagtcgca
     3361 atcgcgagca aaggattcac gttttcagtc ggccacaacg gctggttctg gatcgttcga
     3421 tc
//
LOCUS       DASSFQ010000149         5451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_659361, whole genome shotgun sequence.
ACCESSION   DASSFQ010000149 DASSFQ010000000
VERSION     DASSFQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659361"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="VFH96_05225"
     CDS             complement(<1..1701)
                     /locus_tag="VFH96_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6973403.1"
                     /translation="MIGKTLRNFRITDKLGVGGQGAVYKAVDTKLDRTVVIKVIPPEL
                     SAKEANLKRFEREAKLASSLDHPNICTIYDLDEVEGVHFIAMQFVEGKNVRQLVAGRP
                     LELRTALLIGLQVADALAAAHSRGIIHRDIKSGNVMVTSAGQVKVLDFGLAKLLDDTE
                     AVTAGIHRTELTEVGVPYGTATYAAPEQARGDRVDKRADIFSTGVLLYEMLTGTWPFR
                     GKTTIDVRHAVLHDPPRPIAELRSSAIPPHLDQIIERALAKEPRDRFQNMEDFRSELR
                     KVLHEVDAGGAGYQAVIPEPPRHLSGANPVSRAVRWLKSITRTDSPTSAPNVTPSKSG
                     IHETPFTTIADQEKKSLAILCFRNLSNDPAASFYEFSLADAVITELARVRSLVVRPSS
                     VIARYQGQQRDPREIGQELNVNAVLTAGFIHSGERFRVTAQLIDVASGELLWSDRIDT
                     AASDIIVLQDTIAQRIVEGLSVELSPAEQVGTGKASTKNPLAYEQYLRGRDLFARFIF
                     RTLSRDDCDEAMAHFERAIQLDPNFALAHDGLGACHVNRVFKGFGGSEDYEQAEVAFT
                     K"
     gene            complement(1716..2699)
                     /gene="rfaE1"
                     /locus_tag="VFH96_05230"
     CDS             complement(1716..2699)
                     /gene="rfaE1"
                     /locus_tag="VFH96_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET6973404.1"
                     /translation="MSQLTKTRASEIVRSFHDRNVLVLGDLMLDEFVWGDVTRISPEA
                     PVPVVDVRRESMHLGGAANVLANLVALGARGSVAGVLGNDSAGERLRANLHEMQADQC
                     LIFDMTRPTTTKTRIIAHSQLVVRADRESRVPVPPKIEEMIISCLKEALKKADAFVVS
                     DYDKGVVTPAILREILPLAYEQVPVLVDPKLRNFNSYRPATLVTPNHFEALRMSATED
                     TSDDGSLHAAKVIREKLGCDAVLITRGDRGMMLLEADDRPVFVETAAREVYDVTGAGD
                     TVIATLAAALASGATMLEAATLANHAAGIVVGKVGTATASAEELIGSFAAD"
     gene            complement(2700..3839)
                     /locus_tag="VFH96_05235"
     CDS             complement(2700..3839)
                     /locus_tag="VFH96_05235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6973405.1"
                     /translation="MNSLRDIVELSPQLRVIETQAMYGLQGQDAAQPLAAARWDWSDV
                     RKVLVVRLRSIGDTVLATPSLFALKRFLPAASVDILVEDWVAPVLENHPHVDNVITLE
                     RGGFVARARVARELRSANYDVVYNLHGGTTATFLTRATGARQRVGFKSYQYGQLHNHQ
                     APSPLEIWQQQKAHSVEQQLALLGWTGVPVTDRPRTSLGISPSAAEKVDRLLNDSGLS
                     EQNIALIHPAAAFATKQWAVEHFARVVEFLTERGFTSVAIAAPNEQPLLDQLRSITSG
                     NIVTLALSLPEVTALAARSRLFVGNDSGIAHIAAAVGTPSVVVFGSSNIAHWRPWNSA
                     PAEVVFEEMPCQPCHGYFCAQFAQPECILRVPVARVTAAIDRLLS"
     gene            complement(3844..4014)
                     /locus_tag="VFH96_05240"
     CDS             complement(3844..4014)
                     /locus_tag="VFH96_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET6973406.1"
                     /translation="MAVSQELLEILACPACKAKVELKPDGNALKCVECKRVYPIRDDI
                     PVMLIDEATIED"
     gene            4203..4964
                     /locus_tag="VFH96_05245"
     CDS             4203..4964
                     /locus_tag="VFH96_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973407.1"
                     /translation="MSLEFALQYGCPLRANYSERHLREWGRLSSLHAKLTTGDASAPS
                     IDKLQWVENASPEIERMQEATVICAHCPASLPLDTAGDGESVGCLGRINYPVEAQFEK
                     FLADRVQLSLDTLDKEDQPRLLRILIDPESPFDGEATKELRRVVTDNGLRFFELRVPI
                     VLTREAARLTTDNIFDLLAGFRSEDSERTTYTREFPFEAAADYYDFLDLILRNELSQS
                     EVARLTSRSRNYNQYLRLLTAIEKAEALNARVLID"
     gene            complement(4939..>5451)
                     /locus_tag="VFH96_05250"
     CDS             complement(4939..>5451)
                     /locus_tag="VFH96_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973408.1"
                     /translation="LSSVIMRVCEMLAFQSYNTKSPLNGEAAQWVAQHLGEVPTDKRE
                     FFLDLDQAPDTSATKEALCSNCGGAAAELTQCSAGHALCEYCFVECEKCHQGKVCLVC
                     DKCSQCGPTVCSNCNGKSESLMICDAGHALCEYCAVQCNICNQPKVCLICATCTQCRP
                     RIVSQSELAH"
BASE COUNT         1203 a         1577 c         1482 g         1189 t
ORIGIN      
        1 cttggtgaac gctacttccg cctgttcgta atcttccgag ccgccaaaac ccttgaacac
       61 tcgattcacg tgacacgcgc cgagcccgtc atgcgccaac gcgaagttcg gatcgagctg
      121 gatcgcgcgc tcgaagtggg ccatcgcttc atcacaatcg tcacgagaca aagtgcggaa
      181 tatgaaacga gcaaacaaat cgcgaccgcg caggtactgc tcgtaagcga ggggattttt
      241 cgtcgatgct ttgccagtcc cgacctgttc cgctggactc agctcgacgc tcaaaccttc
      301 gacgatgcgt tgtgcaatcg tgtcctggag cacgatgatg tctgacgcgg ccgtgtcgat
      361 gcgatcgctc cacaacaact cgcccgacgc gacgtcgatc agttgcgcgg tcacgcgaaa
      421 acgctcgccc gaatgaatga agcccgccgt cagcactgcg ttcacgttta gttcttgccc
      481 gatctcgcgg ggatctcgct gttgtccctg atagcgagca atcacggacg acggtctgac
      541 aacgagcgat cgcacgcgcg caagctctgt aatcacggca tcggcaagtg aaaactcgta
      601 aaagctcgcc gccggatcgt tgctcagatt cctgaaacaa agaatcgcga ggctcttctt
      661 ctcctgatcc gcgatcgttg tgaacggagt ttcgtgaatg cctgactttg acggcgttac
      721 attcggcgcc gatgtcggcg agtcagttct cgtgatcgac ttcaaccagc gcaccgcgcg
      781 tgaaaccgga ttggcgccgg aaagatgtcg cggcggctcg ggaatcacag cctgataacc
      841 cgcgccaccg gcgtcaacct cgtgcaaaac cttgcgcaac tcgctgcgaa agtcttccat
      901 gttttggaaa cgatctcttg gttctttcgc gagtgctctc tcaatgatct gatcgagatg
      961 tggtggaatc gcgctcgagc gcaactccgc aatcggtcgc ggcggatcgt gcagtactgc
     1021 gtgtcgcacg tcgatcgtcg tcttgccgcg aaacggccag gtgccggtca acatttcgta
     1081 aagcaagacg ccggtagaga aaatgtcggc acgcttgtcg acgcgatcgc cacgcgcctg
     1141 ttcgggcgcg gcgtaggttg cagtgccgta aggaacaccg acttcggtga gttcggtgcg
     1201 atgaataccg gcggttacgg cttcggtgtc gtcgagcagt ttcgcgaggc cgaagtcgag
     1261 caccttcacc tgacctgccg atgtaaccat cacattgccc gacttgatgt cgcgatggat
     1321 gatgccacgg ctgtgcgcgg cggccagggc gtcggcgact tgcaaaccga tcagtaatgc
     1381 agttctcaat tcaagcgggc gaccggcgac aagctggcgc acgttcttgc cttcgacaaa
     1441 ctgcatcgcg atgaagtgaa cgccttctac ttcgtcgaga tcgtaaatag tgcaaatgtt
     1501 tggatggtcc agcgaagagg caagcttcgc ttcgcgttca aaacgtttga gattggcttc
     1561 tttggcactg agctcgggag gaatgacttt gatgacaaca gtacgatcga gcttcgtatc
     1621 gacggcttta taaaccgctc cctgcccgcc cacaccgagc ttgtcagtga tgcgaaaatt
     1681 gcggagcgtt ttgcctatca tctgcgttat ccgcgttaat ccgctgcgaa ggatcctatt
     1741 aactcttcag cactggcggt tgcagtgccg actttaccga ctacgatgcc ggcggcgtga
     1801 ttggccaagg tggcggcctc gagcatcgtc gcaccagaag cgagtgcggc ggcgagggtg
     1861 gcgataactg tgtctccggc gccggtgaca tcataaacct cacgggccgc agtttcgaca
     1921 aaaaccggac ggtcgtctgc ttcgagcaac atcattccac gatcgccgcg ggttattaac
     1981 accgcgtcac agccgagttt ctcacgaatt actttcgccg cgtgcagcga accgtcgtca
     2041 gaagtgtctt cagtggcgct cattcgcagt gcttcgaagt ggtttggcgt caccagcgtt
     2101 gccgggcgat acgaattaaa gttgcgcagc tttggatcga cgagcaccgg cacctgctcg
     2161 tacgcgagcg gcagaatctc gcgcaagatc gcgggcgtga ccacgccttt gtcgtagtct
     2221 gaaacaacaa aagcgtctgc tttcttcaac gcctctttca aacacgaaat tatcatctct
     2281 tcgatcttcg gcggcaccgg aacgcgtgac tcgcgatcgg cgcgaacaac cagctggctg
     2341 tgagcaatga tgcgcgtttt ggtggtcgtc ggtcgcgtca tgtcaaagat caaacactgg
     2401 tcggcttgca tttcatgcag gttggcacgc aagcgttcgc ccgcggaatc gtttccgaga
     2461 acacctgcaa ccgaaccacg cgccccaagc gcaaccaggt ttgcaagcac gtttgcagca
     2521 ccgccaaggt gcatcgactc gcgccgaaca tcaaccaccg gcaccggcgc ttcgggagaa
     2581 attcgcgtca cgtcgcccca aacaaactcg tcgagcatca aatcgccgag cacaagcacg
     2641 ttacgatcgt gaaatgatcg cactatttcc gaagcacgcg ttttagttaa ttgagacatt
     2701 tacgacagta gccgatcgat cgcggcggtg actctcgcta ccggcacgcg caggatacat
     2761 tcaggttgcg caaactgcgc gcaaaaatac ccgtgacaag gctggcaagg catctcttcg
     2821 aagacgacct cggctggcgc tgagttccat ggacgccagt gcgcgatgtt tgacgagccg
     2881 aatacgacga ctgaaggcgt gccgacagcc gctgcgatgt gtgcaatgcc gctgtcgtta
     2941 ccgacgaaca gtcgtgaacg tgcggccagt gcggtcactt ccggtagcga caaagccaac
     3001 gtcacaatgt ttccggaagt tatcgaacgt aactggtcca gcagcggctg ttcgttaggc
     3061 gcagcaatgg cgactgaagt aaatcctcgt tcagtcaaaa actcgaccac acgcgcgaaa
     3121 tgttcgacgg cccattgctt cgtcgcgaac gcagcagccg gatgaatcag agcaatgttt
     3181 tgttcgctga gtccggagtc gttcagcaat ctatcaacct tctccgccgc gctcggagat
     3241 attcctaaac tagttcgtgg tctatctgta accggaacac cggtccaacc caacaacgcg
     3301 agttgttgtt cgacggagtg tgccttctgt tgttgccaga tctccaacgg tgacggcgcc
     3361 tgatggttat gaagttgacc gtactgatac gacttgaaac cgacgcgctg acgcgcacca
     3421 gtcgcgcgtg tcagaaacgt cgctgtcgtt ccgccgtgaa ggttatagac cacgtcgtaa
     3481 ttcgccgatc gcagctcgcg agctacacgc gcgcgggcca cgaatccgcc gcgctcgagt
     3541 gtgatcacgt tatcgacgtg tggatgattt tcgagaacgg gtgcgaccca atcctcgacc
     3601 agaatatcca cgctcgccgc cggaagaaat cgtttcaagg cgaacagcga aggagtcgcg
     3661 agcaccgtgt cgccaatcga tcgcagacga acaaccagca ccttgcgcac gtcgctccag
     3721 tcccaacgcg ccgcggccag cggctgggct gcgtcctggc cctgcaaacc gtacattgcc
     3781 tgtgtttcga tgacgcgtaa ctgaggagaa agctcaacaa tgtcgcgcag gctgttcatc
     3841 gccttaatct tcgatggttg cctcgtcgat aagcattacc ggaatgtcgt cgcggatggg
     3901 ataaacgcgt ttacattcga cgcacttcag cgcgttccca tctggcttca attcaacttt
     3961 cgctttgcaa gccgggcacg cgagtatttc caagagttcc tgactaactg ccatggacta
     4021 agacctttct aaggcactgc tgcgggaatg cggaaagctt accacaaaga ctgcgacatg
     4081 agcaggttct cactttgcgg ctcctccccc ctttgcgcct ttgcgtgaaa cagtattaag
     4141 agttgtttca cgcaaagacg cagagtttac acaaaggcgc aaagtagacc tatacttcca
     4201 tcatgtcgct tgaattcgca ctacaatacg gctgcccgtt gcgcgccaat tattccgagc
     4261 gtcacctgcg cgagtgggga cggttgagta gtttgcacgc caagctcacg actggcgacg
     4321 cgagtgcgcc ctcgatcgac aagttacagt gggtggaaaa tgcgagtccg gaaatcgagc
     4381 gcatgcagga agccaccgta atttgtgctc attgtcccgc gtctttgccg ttggataccg
     4441 ccggcgatgg cgagtcagtc ggctgtctcg gtcgtatcaa ctatccggtt gaggcgcagt
     4501 ttgaaaagtt tctcgccgac cgcgtccagc tctcgctcga cacgctcgac aaagaagatc
     4561 agccgcgact gcttcgcatt ctgatcgatc cggaatcgcc gttcgatggt gaagcgacga
     4621 aagagttgcg acgcgtagtt actgacaacg gcttgcggtt cttcgagttg cgcgttccga
     4681 tcgtgctgac acgcgaagcg gcgcggctca cgactgacaa catcttcgat ctgctggcgg
     4741 gattcagatc ggaagacagc gagagaacca cctacacacg agaattcccc tttgaagcgg
     4801 ctgctgatta ctacgatttt ctcgatctga ttttgcgcaa tgaattaagc cagtcagaag
     4861 ttgcgaggct gacttcgcgc agtcgcaatt acaatcagta tctccgcctg ctcacggcga
     4921 ttgaaaaagc cgaagcgctc aatgcgcgag ttctgattga ctgacaatcc gcggccggca
     4981 ttgagtacac gtcgcgcaaa tcagacagac ttttggctgg ttacagatat tgcattgaac
     5041 agcgcaatac tcacatagcg catgtcccgc gtcgcagatc attaacgact ccgatttccc
     5101 gttacagttg ctacacaccg tcggtccgca ttgggaacac ttatcgcaaa ctaaacaaac
     5161 cttgccctgg tgacatttct cgcattcgac gaagcaatac tcacacaacg catgacccgc
     5221 actgcattga gttaactccg ccgccgctcc accgcagtta ctgcacagcg cttcttttgt
     5281 cgcactggta tccggcgcct ggtccaggtc gagaaaaaac tcacgcttgt cagtcggcac
     5341 ctcgccgaga tgttgcgcga cccattgcgc ggcttctccg ttcagtgggc tcttcgtgtt
     5401 gtaactttgg aacgccagca tctcgcaaac tcgcatgatt acgctgctga g
//
LOCUS       DASSFQ010000150         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_803706, whole genome shotgun sequence.
ACCESSION   DASSFQ010000150 DASSFQ010000000
VERSION     DASSFQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803706"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1716)
                     /locus_tag="VFH96_05255"
     CDS             complement(127..1716)
                     /locus_tag="VFH96_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6973409.1"
                     /translation="MAKKPFSWICSLLLVLCLTHTGCFNTNEPSPYYGKVVTPKTQEF
                     RWSNGGLPQTFDPAFAAAPPDTDAVRALFEGLTDYDPKTLAPVPAVATRWESSNDGRV
                     WTFYLRDNARWSNGEKVTAQDFVRSWERTLKIGPLAPHTELLANIEGATPSVSEPHIF
                     GARALDENVLQVTLQHADSSFPALVAHPVFRPVKVADAQRTNRLESHELISNGAFHLL
                     ATENNQVQLERAKTYWDDASVALDRVTFVNATNAEDALSAYESGEIDAVTNAPFEPLA
                     LKLLTPYKDFHRSTFGALTYYAFNTAHEPFDDVRVREALALAIDRERVSRDDLGGATE
                     PAGKFLPEAMSGEQPVVDKAGLVEHDVNKARELLAEAGYPNGEGFPVIKLLINRNEQQ
                     RIVAQSIVAMWRVALNIDAEIVIRNWDEYEAAIKTGDYDIVRRGMVMQSTSELVNLRM
                     IFERDTRPLPSASPEASPSPQPTLVVETEAQALKDMKAVPIYFASSYSLVKPYVSGFD
                     SNVLDVPSLKKTRIATNWAQP"
     gene            complement(1709..2317)
                     /gene="coaE"
                     /locus_tag="VFH96_05260"
     CDS             complement(1709..2317)
                     /gene="coaE"
                     /locus_tag="VFH96_05260"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET6973410.1"
                     /translation="MLRVGLTGSIGVGKSFVALVFIELGCHVLDADQTAREVVMPGTE
                     GLKALTDAFGEEILNTDGTLDRKQLGALIFADPSQRQRLNHILHPFIIARQDEIMNGW
                     EAEDPDGIGIIDAALMIESGGYKRFDKLIVVHCRPEVQLERLMLRDQLSRDEALRRIN
                     SQMPQEEKQKFADYLIDTSDGYESTRAQTEAVNQKLIRVIRG"
     gene            complement(2323..3225)
                     /locus_tag="VFH96_05265"
     CDS             complement(2323..3225)
                     /locus_tag="VFH96_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET6973411.1"
                     /translation="MIKLEAAGLAARIKEEVKAEIERGVRASLAAVRVGDDPASAVYV
                     RNKIRACEEVGIRSEQHALPESTTTEQLLDLITSLNTRDDVDGILTQLPLPAHIDETA
                     IIEAMNPLKDVDGFHPVNAGKLAMGRPVFVPCTPAGIIELLDDRGVEIRGANACVVGR
                     SQIVGRPMAQLLLQRDATVTICHSRTRDLPAVTRTADLLIVAIGRAGFVGRDHIKPGA
                     IVIDVGMNKVSDVDEVRRLFGDEAEKRLEVVAKRGSTLVGDVNPAEAAEVAGMLTPVP
                     GGVGLLTVAMLMKNTLKAAKLRRL"
     gene            3342..>3971
                     /locus_tag="VFH96_05270"
     CDS             3342..>3971
                     /locus_tag="VFH96_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973412.1"
                     /translation="MSLRLVRIAARLILFIAALNMAAFAVAAQSADPIVAAIEAEDWQ
                     TARAEIGKVRAANEAIFRDKNYEYLLGRIAERTGDTAGAVANYQAIASNNSRLKEYAL
                     WRLSKLARATGDLVLERERLQQLIAAAPSSLLFDAATLRLSESFFESGDFAAAANSTK
                     SLTQSKNINMSREGAALMGLAYVRSGKTAEARDVFTKLVMQMPDASRPDD"
BASE COUNT          862 a         1190 c         1077 g          842 t
ORIGIN      
        1 ccgcggcaac agtgcgcgtc tcttccctac ccctacgaac gacactaaac tcagtcatat
       61 ttttaagccg cggattaacg cagatgaacg cagatcagcg tttatccgcg tgaatccgcg
      121 gctaaattaa ggttgagccc agtttgtggc gatgcgtgtc ttcttcaaac tgggaacatc
      181 caacacattc gaatcaaacc cggaaacata cggcttcacg agcgagtacg aagacgcaaa
      241 gtagatcggc accgccttca tatctttcaa cgcctgcgcc tccgtctcaa ctaccaaggt
      301 gggctgcggc gagggactgg cctccggcga agcagatggc aatggacgcg tatcgcgttc
      361 aaatatcatg cgcagattca ccaactcact cgtcgactgc atcaccatcc cgcgacgcac
      421 gatgtcgtag tcaccagtct tgatcgcggc ttcatactca tcccaattac ggattacgat
      481 ctccgcatcg atattcaacg caactcgcca catcgccacg atcgactgtg ccacaatcct
      541 ttgctgctcg ttgcgattga tcaaaagctt aatcaccgga aagccttcgc catttggata
      601 accagcttca gcaagcagct cgcgcgcttt gttgacgtcg tgctcgacta acccggcctt
      661 atcaaccacg ggctgttcac ccgacatcgc ttccggaaga aatttaccgg ccggctcggt
      721 tgcaccgccg agatcgtcac gactcacacg ttcgcgatcg attgcgagcg cgagggcctc
      781 gcgcacacgc acatcgtcaa acggctcgtg cgcggtgttg aaagcgtagt aagtcaaagc
      841 gccgaacgtt gagcgatgga agtctttgta aggcgtgagc agcttgagcg ccaacggctc
      901 gaacggcgcg tttgtcacgg catcgatctc tcctgactca tacgcagaca acgcatcttc
      961 agcgttcgtg gcattcacaa acgtgacgcg atcgagcgcc actgacgcat cgtcccagta
     1021 tgttttggcg cgttcgagct gcacctgatt attctcggtc gcgagtagat gaaaagcgcc
     1081 gtttgagatg agttcgtgtg attcgagacg gttcgtgcgc tgtgcgtctg cgactttgac
     1141 gggacgaaaa accggatggg ccacgagtgc gggaaaacta ctgtctgcat gttgcagcgt
     1201 cacctgcagc acattctcgt cgagcgcgcg cgcgccgaag atatgtggct ccgagacact
     1261 cggagtcgcg ccctcgatgt ttgccagcag ctcggtgtgc ggcgcgagtg gaccgatctt
     1321 gagcgtgcgc tcccacgagc ggacgaagtc ctgcgccgta accttctcgc cattggacca
     1381 tcgcgcgttg tcgcgtaagt aaaacgtcca gacacggcca tcgttcgacg attcccagcg
     1441 cgttgcgacc gccggcacgg gcgcgagtgt ctttggatcg tagtcagtca gaccttcgaa
     1501 gagagctctc acggcgtctg tatcaggcgg cgcagcggcg aaggcgggat cgaatgtctg
     1561 cggcagaccg ccattgctcc agcgaaactc ctgcgtcttc ggggtcacga ctttgccgta
     1621 gtacggcgaa ggctcgttgg tgttgaagca gccggtgtga gtcagacaca gcaccagcag
     1681 cagcgagcag atccaggaga aaggtttttt agccacggat gacacggatt aatttctgat
     1741 ttaccgcctc ggtttgagcg cgcgttgatt cataaccgtc ggatgtgtcg atcaaataat
     1801 cggcgaattt ctgtttctct tcctgcggca tctgcgagtt gatgcgccgc agcgcttcgt
     1861 cgcgcgagag ctggtcgcgc agcatcagtc gctcgagctg cacttcagga cggcaatgga
     1921 ccacgatcag tttatcaaag cgcttgtagc cgcctgactc gatcatcagc gccgcatcga
     1981 tgatgccgat gccgtccgga tcttcagcct cccacccgtt catgatctca tcctgacgcg
     2041 cgatgatgaa cgggtgcaga atgtgattga gacgctggcg ctggctcgga tcagcgaaaa
     2101 tcaatgctcc gagctgtttc cgatcgagcg ttccatccgt gttcaggatt tcctcgccaa
     2161 acgcgtccgt cagcgccttc aaaccctcgg tgcccggcat gacgacttca cgcgccgttt
     2221 gatcggcatc gagaacgtga cacccgagct cgataaagac caacgccaca aacgatttgc
     2281 cgacaccgat tgagcctgtg agtccgacgc gtaacattca gatcacaacc gtcgcaactt
     2341 ggcggctttc aatgtgttct tcatcaacat cgccaccgtc aacaatccga cgccaccggg
     2401 cacgggcgtc aacatgcccg cgacttccgc cgcctcggcc ggattcacat cgccaacgag
     2461 agtcgaaccg cgtttggcga caacctcgag tcgcttctct gcttcgtcac caaatagcct
     2521 gcgcacctca tcgacgtcag acaccttatt catgccgaca tcgatcacga tcgcgcccgg
     2581 cttgatgtga tcgcggccga cgaagcccgc tcttccgatc gcaacgatga gcaggtctgc
     2641 cgtgcgcgtc accgccggca ggtcgcgcgt gcgcgagtga caaatcgtca ccgtggcatc
     2701 gcgttgcaac agcaactgcg ccatcggacg tccgacaatc tggctgcgac ccacgacaca
     2761 cgcattcgcg ccgcgaatct cgacgccacg atcatctagt agctcgatga tgccggcggg
     2821 cgtgcaaggc acgaagaccg gtcttcccat cgcgagcttg cccgcgttca ccggatgaaa
     2881 gccatcgaca tccttcaaag gattcatcgc ttcgatgatt gcagtttcgt caatgtgagc
     2941 gggcaatgga agctgcgtca ggatcccatc gacgtcgtca cgcgtgttca atgaagtgat
     3001 gagatcgagt agctgctcag tcgttgtcga ctcaggtaac gcgtgctgtt ccgagcgaat
     3061 gccgacctct tcacatgcac gaattttgtt tctgacatac acagccgacg ccggatcgtc
     3121 gccgacacgc acggcagcca gcgacgcgcg cacgccgcgc tcgatctccg ccttaacttc
     3181 ttctttgatt ctggcagcca gtccggccgc ttcaagtttg atcatattaa tagccacaaa
     3241 aaagcgcaca agacacaaac caaccctaca tcgagccgag attcgaaaat taatggtctg
     3301 tgctctttgt gcccttctgt ggcgtcatta taatccgagt tatgtcgtta agactcgtta
     3361 gaatagctgc cagactaata ctttttatcg ccgcgctcaa catggccgct tttgccgtcg
     3421 cggcccaatc ggcggatccg atcgtcgcgg cgatcgaagc cgaagactgg cagactgcgc
     3481 gcgccgagat cggcaaagtt cgcgcagcaa acgaagcgat ctttcgcgac aagaattacg
     3541 agtacctgct cggccgcatt gcggaacgaa ccggcgacac cgccggcgct gtcgcaaact
     3601 accaggcaat cgcgtcaaac aactcgcgat tgaaagagta tgcattgtgg cgactctcga
     3661 agctcgcgcg cgcgactggc gatctcgtgc tcgagcgtga gcgtttgcaa caactcatcg
     3721 ccgcagcgcc atcgagtctg ctctttgacg cggcgacact gcggttgagc gagagttttt
     3781 ttgagagcgg tgatttcgct gcggcagcga atagtacaaa gtctttgacg caatcgaaga
     3841 acatcaatat gtcgcgtgaa ggcgcggcgt tgatgggcct cgcgtacgtg cgctctggaa
     3901 agacggccga agcgcgcgat gtcttcacga aattagtgat gcagatgccg gatgcgtcgc
     3961 gtcctgacga c
//
LOCUS       DASSFQ010000151         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_803881, whole genome shotgun sequence.
ACCESSION   DASSFQ010000151 DASSFQ010000000
VERSION     DASSFQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803881"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFH96_05275"
     CDS             <1..731
                     /locus_tag="VFH96_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973413.1"
                     /translation="AANGFSNRIEFFENVSTEITLPEPATIIISDLRGVLPWFEQNVS
                     SIIDARKRLLAPEGVLIPQRDILWAAVVEAPQQYAELIDPWHDQFDLDLSAATRLVIN
                     TWRKSRIRSEQLLTEPVCWTTLDYYEVNSPDVHADISWQAAHNGTAHGLAVWFDSELA
                     EGIRFSNHPAAPEMIYGIGFFPFSQPVAVTEGERINIRLAADLVNDGYVWRWDTEVPG
                     KASFKQSTFFGAPLSTSNLRKSAQ"
     gene            735..2600
                     /locus_tag="VFH96_05280"
     CDS             735..2600
                     /locus_tag="VFH96_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973414.1"
                     /translation="MFNSIRILSIKLRKALAQLPYLPRALSLVWEVGRSWTVAWIVLL
                     IVQGLLPAAFVYLTKLIVDALIIAVRIGGSWPEMRHVLVLVILLGGVLLLMEVVRNAI
                     NWVRTVQAELLQDHITALIHEKSVTVDLAFYELADYYDHLHRARTEARYRPVALLGNL
                     GALLQNSITLIAMGAILIPLGPWLTVALLLSTLPAFFVVVHYALAEYQWRQRTTTDDR
                     RAWYYDWIMTTAEAAAELRLFGLGKYFQAHHKDLRRKLRGERLELTRRQGLAELAASM
                     IALLIIGAAMVLMMWRALNGSVTLGELALVYAAFNQGQGLMRTLLENAGQLYGNSLFL
                     GNLFEFLSLRENSQTAERTVGAAPRGRPSSAPTISFQHVSFAYPDATNNALDNFTMSI
                     PRGKIVALVGANGAGKSTLVKLLCRFYDTNAGSIEIDGQDIKQIPIDDLRRSITVLFQ
                     QPFHYNTTMRDNILYGDLNLDASDAELDAAIRAAGASEIVARLPNREQTLLGRWFPGG
                     TELSVGEWQRIALARAFLRRASIIILDEPTSALDPWAEADWLERFRQLAFDRTSIIIT
                     HRFTTAMHADVIHVMDRGRIVESGSHRLLLEQNGLYAESWYRQNLLAGDYTNASR"
     gene            2627..>2973
                     /locus_tag="VFH96_05285"
     CDS             2627..>2973
                     /locus_tag="VFH96_05285"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6973415.1"
                     /translation="MSVARREVRELHVPPQLDWDYVLDTARANGLLPLLQKHLSNAAS
                     GVLPGHVLSSLKREAVANLQSMLHLVGKQLRVYKLFQEQGIPVAIFKGALLAQMAYGE
                     ISLRHAGDIDLLI"
BASE COUNT          637 a          885 c          793 g          658 t
ORIGIN      
        1 ccgcggcaaa tggtttctca aatcgcattg aatttttcga gaacgtgtcg acggagatca
       61 cgcttcccga accggcaaca atcatcattt ccgatctccg tggtgtcctg ccgtggtttg
      121 agcaaaacgt gtcgtcaatc atcgacgcac gcaaacgatt attggcgccc gaaggcgtgc
      181 taattccgca gcgcgacatt ttgtgggcgg cagttgtcga agctccgcag caatacgctg
      241 agctgatcga tccgtggcac gatcaattcg atctggatct gtcggcagca acgcgtctcg
      301 ttatcaacac ctggcgcaag tcgagaataa gatccgaaca gttgctgacc gagccggtct
      361 gttggaccac gctcgattac tacgaagtca atagtccgga cgtccacgcc gatatttcct
      421 ggcaggctgc acacaacggc actgctcatg gattggcggt ttggtttgat tccgaactcg
      481 cggaaggtat tcgtttttcg aatcacccgg cggctccgga aatgatctac ggtatcgggt
      541 tctttccatt ttctcagccg gtagcagtga cggaggggga gcggatcaat attcgactcg
      601 ctgccgatct cgtgaacgac ggttacgttt ggcgttggga tactgaagtt cccggcaaag
      661 cgagcttcaa gcagtcgacc ttctttggcg cgcccctatc aacgagcaac ttgcggaagt
      721 cagctcagta gacgatgttc aattcaatcc ggattctgag cattaagctt cgaaaggcgt
      781 tggcgcaact gccttatctg ccgcgcgcgt tgtcgctcgt ctgggaagtc ggaagatcgt
      841 ggacggtcgc gtggatcgtg ctgctgatcg tgcaggggct gttgccggct gcctttgttt
      901 atctcacgaa gctcatcgtc gatgcgctca tcatcgcggt tcgaattggc gggtcgtggc
      961 ctgagatgcg gcacgtgctg gtgctcgtca tcttgctcgg cggcgtgttg ttgttgatgg
     1021 aagtagttcg caacgccatc aactgggttc gcaccgtaca ggccgaactt ttacaagacc
     1081 acatcaccgc actgattcat gaaaaatcag taacagtcga tcttgctttc tacgaactcg
     1141 ccgattacta cgatcacctg caccgcgcac gcactgaagc tcgttatcgg cccgtggcgc
     1201 tgcttggaaa tctcggcgcg ttactgcaaa acagcatcac gctaatcgca atgggtgcca
     1261 tcctgattcc gcttggcccc tggctaactg tcgcgctcct gctcagcacg ttgccggcgt
     1321 tcttcgtcgt agtccactac gcgctcgcgg aataccaatg gcgacagcgc acaactactg
     1381 acgatcgccg tgcgtggtat tacgactgga tcatgaccac agccgaagcg gctgcggagc
     1441 tgcgactctt cggactcggc aagtattttc aggcgcatca caaagacctg cgccggaagc
     1501 tacgaggcga gcgtttggaa ctgacgcgcc gtcagggtct ggcggaatta gcagccagca
     1561 tgattgcgtt actgatcatc ggcgccgcga tggtgctgat gatgtggcga gctctaaacg
     1621 gctccgtcac actcggcgaa cttgcactcg tctacgccgc ctttaaccag ggccagggcc
     1681 tcatgcgaac actcctggaa aacgccggcc aactctatgg caacagcctc ttcctcggta
     1741 acctcttcga attcctctcc ctaagagaga attcacagac tgccgaacgt actgtagggg
     1801 cggccccccg tggccgcccc tcgtctgccc caacgatctc tttccaacac gtctccttcg
     1861 cctacccgga cgcgaccaac aacgcgctcg acaacttcac catgtcgatc ccacgcggca
     1921 agatcgtcgc actcgtcggc gcaaacggcg cgggcaaaag caccctggtc aaacttctct
     1981 gtcgtttcta cgacacaaac gccggctcaa tcgaaatcga cggtcaggac ataaaacaaa
     2041 tcccaatcga cgatttgcgc cgctcaatca ccgtcttgtt ccaacaacca tttcactaca
     2101 acaccaccat gcgcgataac atcctctacg gcgatctaaa tctggacgca tccgacgccg
     2161 agctcgatgc cgcaatccgc gccgcaggcg cctcggaaat cgttgcgcgt ctgccgaatc
     2221 gagagcaaac actcctcggc cgctggtttc caggcggcac cgaactcagc gtcggcgagt
     2281 ggcaacgcat cgcgctggca cgcgcgtttc tgagacgcgc ttcgattatt attctcgatg
     2341 agccaacgag cgcactcgat ccgtgggccg aagctgactg gctcgagcgt ttccgccaac
     2401 tcgctttcga ccgcacgtcg atcatcatca cgcaccgatt cacgactgca atgcatgccg
     2461 acgtgattca cgtgatggac cgcggacgca tcgtcgaatc aggcagccat cgactattgt
     2521 tggaacagaa tggactgtat gccgagtcgt ggtacaggca gaacttgttg gccggtgatt
     2581 acacaaacgc ttccagatga atcacagccg tgaaaatgat ttgctcttga gcgtagcgcg
     2641 gcgtgaagtt cgcgagctgc acgtgccgcc gcaacttgac tgggattatg tcttagacac
     2701 agcacgagcc aacggacttt tgccgctctt gcaaaaacac ttgagcaacg cagcgagcgg
     2761 agtcttgcct ggtcacgttc tttcgtctct caaacgtgaa gcggttgcga atttgcagag
     2821 catgctccac ctcgtcggca agcaattgcg cgtttacaag cttttccagg agcaaggcat
     2881 tccggtcgcg atcttcaaag gcgcgctcct cgcgcagatg gcgtacggcg agatatcgct
     2941 ccgccacgcc ggcgacatcg atctgctgat caa
//
LOCUS       DASSFQ010000152         6585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1557412, whole genome shotgun sequence.
ACCESSION   DASSFQ010000152 DASSFQ010000000
VERSION     DASSFQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557412"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..729)
                     /locus_tag="VFH96_05290"
     CDS             complement(49..729)
                     /locus_tag="VFH96_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6973416.1"
                     /translation="MSTVSNNGTAKREDPRAAASTTMLPQWALNKIRPVVGAFSRLFW
                     KIEFVGVENVPERGGVIVAANHQTYIDPFWLSLPIKRPTRYLAWSAAFRWPIVGTGLK
                     LLGAWPLALEGSDPAAIRTSLQWLRNGGVVVIFPEGARSTDTGALDRFKAGAVRLAME
                     AQVPILPVTIRGGNRVWPRGWRFPRFGKVVVTYHPLYHPEQCSNEETRACARRESEKL
                     AQVIASAL"
     gene            complement(747..1463)
                     /locus_tag="VFH96_05295"
     CDS             complement(747..1463)
                     /locus_tag="VFH96_05295"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6973417.1"
                     /translation="MSEQRNFKRIRERLPLSLPVRVRGRETPTFEWTEVTRLTNVTPF
                     GAGFTLRHPTEKGRLLHMTIPMPRQLRVFDHVEDQYRIWAVVRYIKTTIVENKTVFEV
                     GVAFIGKRPPASYEKEPWTLYDVVNTTFEAAKSADEIVIPFADQREYTRHHMAVDMRI
                     EIVDEAGNVLEQEQTVTENISTKGATLFTTLEISHGRFVRLTSEQYRITVHAAIRSRS
                     KGADGVPRIHVEFIDKEWPL"
     gene            1708..4272
                     /gene="gyrA"
                     /locus_tag="VFH96_05300"
     CDS             1708..4272
                     /gene="gyrA"
                     /locus_tag="VFH96_05300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET6973418.1"
                     /translation="METTSERNHINIEDEMRRSYLDYAMSVIIGRALPDVRDGLKPVH
                     RRVLWAMHELGNTYNKAYKKSARIVGDVIGKYHPHGDMPVYDTIVRLAQDFSMRYPLI
                     DGQGNFGSVDGDAAAAMRYTEIRMARLANEVLADIEKETVTFQPNYDESLSEPTVLPA
                     RVPNLLINGSDGIAVGMATKIPPHNLTEIVEATLALLKDPETTVQDLTKIVTGPDFPT
                     GGFIYGRDEIRKSYEEGRGVLQIRARAGIDRIGRGAQERDAIVITEIPFQVNKARLLE
                     RIAELINEKKIDGISDLRDESDRTGMRIVVELKRDAVPQVVLNKLYKLTPMQSSFGVI
                     NLAIVNGQPRVLNLKQMLECFIEFRREVVRKRTEYELRKARARAHILEGLTKAIDALD
                     AIILLIRNSRSVDEARQWLMGQLQTTSEVKTWKGVPSDVQLKTYLGRIQKTMEGLAFS
                     EIQAQAILDLQLRRISALERQKIIDEYEQVIKHIAELEEILGNERSLRRVIAKELEEV
                     KKDFGDGRRTEIVDEGVELSIEDLIADEDVAITVTNSGYIKRTPITTYSRQGRGGKGR
                     FGAAAKNGDFVEHLFIASTHAYLMIFTDDGMVYKIKVHEVPDAAASARGKAVVNLINI
                     PSDRRLAGVVPVREFAEGRHVVMVTRKGVIKKTSLADFQNIRTNGIIAINVDEGDQLL
                     DVVLTDGTKRIFIATHNGLAIRFDEKNVRPMGRATRGMRGIDLRQDDYVVSVCPVSAE
                     DKERMLSVSEQGYGKQTSITTYRLQSRGGKGVINMKTTEKTGKVVAVFPVEDESEIMI
                     ITQQGKLIRLEAADIRKTGRSAQGVRLIKTDAGDKVTSASLVEAAAEEETEESPAG"
     gene            complement(4429..5100)
                     /gene="moaD"
                     /locus_tag="VFH96_05305"
     CDS             complement(4429..5100)
                     /gene="moaD"
                     /locus_tag="VFH96_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HET6973419.1"
                     /translation="MKIRVLFFGAARDAVGANQMDLALEAPATVSSAFQSLQERFEKL
                     ERFGRSLLFAVNQEYATPDTQLKENDELAIFPPVSGGSHDFFELTTEPIDVGEVARRV
                     VLPECGATVTLDGYAREWTKGKRTLYLVYEAYDSMALTEMQRLGAEAHQQFDIAHIGI
                     VHRTGRIEIGETSVVIAVSAPHRKAAFQACEWAIKELKRTVPIWKKEIFENGEEWVTP
                     QTGTE"
     gene            complement(5097..5798)
                     /locus_tag="VFH96_05310"
     CDS             complement(5097..5798)
                     /locus_tag="VFH96_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET6973420.1"
                     /translation="MSEASDKVLGQRELPLFPLPVVLFPGVPLPLHIFEPRYRQMLLD
                     IRLGKNLFGLSYFDPTTSENEVPPKGHVGCVAEVTETQTFPDGRSNVLTIGVIRYRIE
                     SYVESGDPYLVAQVSYFEDEDEDEELLAAPAREVAETFTRIAQAVRTINDERATLPDI
                     SNTEPQRLSFLVAAAMEIDAEVKQEMLELRSTSERLERLRSMLSIAVKGYEERARIHE
                     LAKSNGHGGKRIEIE"
     gene            complement(5984..6388)
                     /locus_tag="VFH96_05315"
     CDS             complement(5984..6388)
                     /locus_tag="VFH96_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973421.1"
                     /translation="MKPRILIVDDDSQITQQLYWTLCDEYDVVTANDMQTAIRRATFY
                     KPDISILDLQMPPDKDSPAMGLRLIEYIKGHLANSKVLIMSSNTDPEVRKACVNAGAD
                     GFFAKPFELEDMLASICRLTPVHRLELFAQIL"
BASE COUNT         1495 a         1845 c         1828 g         1417 t
ORIGIN      
        1 cacaaaagag taagtaaaat cttttgtgct ttttgtgcct ttttgtggct ataaagctga
       61 agcgatcact tgtgcgagct tttcactttc ccggcgcgcg cacgcacgcg tctcttcatt
      121 tgaacactgc tccgggtggt agagcgggtg ataggtcacc acgacttttc cgaacctcgg
      181 aaagcgccag ccgcgcggcc acacacgatt tccacccctg atcgtcaccg gcagtatcgg
      241 cacctgcgcc tccatggcta aacgcaccgc acctgcttta aatcgatcca gcgcaccggt
      301 atccgtactc cgcgcaccct ctggaaagat caccaccaca ccaccatttc gcagccattg
      361 caacgatgta cgtatcgccg cgggatcgct cccctcgagc gcgagtggcc acgcgccgag
      421 caacttcaga cccgtcccca caatcggcca gcggaacgcc gcactccacg caagataccg
      481 ggtcggtctc ttgatcggta gcgaaagcca gaacggatcg atgtacgtct ggtgattcgc
      541 cgcgacgatc actccaccac gctccggcac gttctcgaca ccaacaaatt caatcttcca
      601 gaacaaacgg ctaaacgcgc caacaacggg ccggatcttg ttcagcgccc attgcggcag
      661 catggtggtg ctcgccgcgg cgcgcggatc ctctcgttta gcggtgccgt tgttacttac
      721 tgtactcacg gttatgggtg gttatcttac aacggccatt ccttatctat gaactcgaca
      781 tgaattctcg gaacgccgtc cgcgcccttg ctgcgcgagc ggatggctgc gtgcacggtg
      841 atccggtact gctcgcttgt caaacgaaca aaacggccgt gcgagatttc gagggtggtg
      901 aaaagcgtcg cgcccttagt gctgatgttc tccgtcactg tttgctcttg ttcaagcacg
      961 ttgccggctt cgtccacgat ctcgatgcgc atatcgacgg ccatgtggtg tcgtgtgtat
     1021 tcgcgctgat cggcaaaggg tatcactatc tcgtcagcag actttgccgc ctcgaaggtg
     1081 gtgttcacga cgtcataaag cgtccatggc tctttctcgt aactggccgg cggacgcttg
     1141 ccgatgaacg cgacgccaac ttcaaacacg gtcttgtttt caacaatcgt cgtcttgata
     1201 taccgcacga cggcccagat ccggtactgg tcctccacat gatcgaacac gcgcaactgc
     1261 cgcggcatcg ggatcgtcat gtgcaacagg cgaccctttt cggtgggatg tctgagtgta
     1321 aaaccggcgc caaacggggt cacattcgtg agccgcgtca cctcggtcca ctcaaaagtc
     1381 ggcgtctcgc ggccacgaac acgcacaggt aacgagagcg ggagtcgctc gcgaatacgc
     1441 ttaaaatttc gctgttcact catgggagcc gacggacgtg agaacggatc cgaaaacgtc
     1501 gggagtggtt acacgaagct attgatttta ttagagtttt cgcctatttc gtgtttgcca
     1561 atcacactaa aaaaaggcta aaatacaggc tactttgagc cctatgcttc accggttttt
     1621 tctcccccga aaagcgccaa actcaagctc ggtatctctt cagcgggggc caaactgtgt
     1681 cgcggattgt actggaaagg ctattagatg gaaacaactt ctgaacgcaa tcatataaac
     1741 atcgaagacg agatgcggcg gtcgtatctc gactacgcga tgtccgtaat tatcggccgc
     1801 gcgctaccgg atgttcgcga cggattgaag cccgtgcacc ggcgcgtttt gtgggccatg
     1861 cacgagctcg gcaacactta caacaaggcc tacaagaaga gcgcgcgcat cgtcggcgac
     1921 gttatcggta agtatcatcc gcacggtgac atgcccgttt acgacacgat cgtgcgcctg
     1981 gcccaggact tctcgatgcg ctacccgctc atcgacggac agggcaactt cggatcggtc
     2041 gacggcgatg cagcagcggc gatgcgttac acggaaatac gcatggcgcg tttggcgaat
     2101 gaagttctgg ctgacatcga gaaagaaacc gttacctttc agccaaacta cgacgagtcg
     2161 ctcagcgagc cgacagtttt accggcgcgc gttccgaacc ttttgatcaa tggttcagac
     2221 ggcatcgcgg tcggaatggc gacgaagatc ccgccgcaca atctcaccga gatagtggaa
     2281 gcgacgttgg cgttgttgaa agatcccgag acgactgttc aggacctcac gaagatcgtg
     2341 actggtcctg actttccaac cggtggattt atctacggtc gcgacgagat caggaaatcg
     2401 tatgaagaag gccgcggcgt gttgcagata cgcgcgcgcg ccggcatcga tcgcattggc
     2461 cgcggcgcgc aggagcgcga tgcgatcgtc atcaccgaga ttccgttcca ggtcaacaaa
     2521 gcccgcttgc tcgaacgcat cgccgagctg atcaacgaga agaagatcga cggtatttcc
     2581 gatctgcgtg acgaatcgga tcgcaccggc atgcgcatcg tggtcgagtt gaaacgcgat
     2641 gcggtgccgc aagtcgtgct caataagctc tacaagctga ccccgatgca gtcgtcattc
     2701 ggcgtcatca atctcgcgat cgtgaatgga cagccgcgcg tcttgaatct caagcagatg
     2761 ctcgagtgct tcatcgagtt tcgtcgcgag gtcgtgcgga agcgcaccga atacgagctg
     2821 cgcaaggccc gcgcccgtgc acacattctc gaagggctca cgaaggcgat cgacgcgctc
     2881 gacgccatca tcttgctcat ccgtaattca cgttcggttg acgaagcgcg acagtggctg
     2941 atgggccagt tgcaaactac gagcgaggtc aaaacgtgga agggcgttcc gtctgacgtt
     3001 cagctcaaga cttatttggg tcgcatacaa aagacgatgg aaggtctcgc cttctctgaa
     3061 attcaggcgc aggcgatcct cgatctgcaa ctgcgacgga tatcggcgct cgagcgacag
     3121 aagatcatcg acgagtacga gcaggtgatc aaacacattg ccgagctcga agagattctc
     3181 gggaatgagc gttcgctgcg acgcgtcatc gccaaagagc tcgaggaagt taagaaagac
     3241 ttcggtgatg ggcgccggac ggagatcgtc gacgaaggcg tcgagctctc gatcgaggat
     3301 ctaatcgctg atgaagacgt ggcgatcact gtcacgaaca gcggttacat caagcgcacg
     3361 ccgatcacga cttattcgcg tcagggacgc ggtggtaaag gacgcttcgg cgctgcggcg
     3421 aagaacggcg acttcgtcga gcatctcttc atcgcgtcga cgcatgcgta cctgatgatc
     3481 ttcactgacg acggcatggt ctacaagata aaagttcacg aagtgcctga tgcggcggcg
     3541 tccgcgcgcg gcaaggcggt cgtgaatctc atcaacatac cttcagatcg aaggcttgcg
     3601 ggtgtggttc cggtgcgtga gtttgcagaa ggccgacacg tcgtgatggt gactcgcaag
     3661 ggcgtgatca agaagacatc gctggctgat ttccagaaca ttcgcacaaa tggaatcatc
     3721 gcgatcaatg tcgatgaagg cgaccagttg cttgacgtgg tgttgactga cggcacgaaa
     3781 cgcatcttca tcgcgacgca caacgggctc gcgatccgat tcgacgagaa aaacgtacgg
     3841 ccgatgggac gtgcgacgcg cggcatgcgc gggatcgacc tgcgacagga tgactacgtc
     3901 gtttctgttt gcccggtatc agcagaagac aaggaacgga tgctgtcagt ttccgagcaa
     3961 ggctacggca aacagacttc gatcacgact tatcgactgc aatcgcgtgg cggcaaaggc
     4021 gtgatcaaca tgaagacgac tgaaaagact ggaaaggtag ttgcggtgtt tccggtcgaa
     4081 gatgagtcgg agatcatgat catcacgcag cagggcaagc tcattcgcct cgaagccgcc
     4141 gacattcgca agaccggtcg cagcgcccag ggcgtgcgcc taatcaagac tgatgctggc
     4201 gataaagtga cgagcgcatc attggtcgaa gccgcagccg aggaagagac tgaggagtca
     4261 ccggcgggct aagccacaaa aagcacagaa gaaagttagc cacgaaaagg cacaaaagac
     4321 acaaaaagtc tttttgagaa gttaggcgcc tgttctccca cgcgagaatc aggcgcctta
     4381 cctgtttaag aatctttttt gtgtcttttg tgcctttttg tggctaactt attctgttcc
     4441 cgtttgcggc gttacccatt cctcaccatt ctcgaaaatt tcttttttcc agatcggcac
     4501 ggtgcgtttg agctccttga tggcccattc gcaggcttga aatgcggctt tccggtgagg
     4561 ggcgctgacg gcgatcacga cgctcgtttc gccgatctcg atgcggccgg tgcggtggac
     4621 gatgccgatg tgcgcgatgt cgaattgctg gtgggcttcc gcgccgaggc gctgcatctc
     4681 cgttaacgcc atcgagtcgt aagcttcata gaccaagtat aacgttcgtt tgcctttggt
     4741 ccactcgcgc gcgtaaccat cgagcgtgac agtcgcaccg cattccggca gcactacccg
     4801 ccgcgctacc tcacccacat cgatcggctc cgtagttaat tcgaagaagt cgtgcgatcc
     4861 accactaacc ggtggaaata tcgccagctc atcattctct ttcaactgcg tatccggtgt
     4921 ggcgtattcc tgattcaccg caaacaacaa cgaccgccca aaacgctcga gcttctcgaa
     4981 cctctcctgc agactctgaa acgcactcga aacagttgcc ggagcctcga gcgccaaatc
     5041 catttgattg gctcccaccg catcacgcgc cgcaccgaag aacaacaccc gaatcttcat
     5101 tcaatctcga tccttttgcc gccatgccca ttactcttgg ccagctcgtg aattcgcgca
     5161 cgctcctcat accctttgac cgcaatgctc aacatcgatc gcaatctttc gagccgttcc
     5221 gacgtcgatc gaagctcgag catctcctgt ttgacctcgg cgtcgatctc catcgccgcg
     5281 gcgacgagaa acgagagccg ctgcggctcg gtgttcgaga tgtcgggcaa cgtcgcgcgc
     5341 tcgtcgttga tggtccgcac tgcctgcgcg atgcgcgtga atgtttcggc tacttctcgc
     5401 gccggcgcag caagtagctc ttcgtcctcg tcctcgtctt cgaagtaact gacctgggcc
     5461 acgagatacg gatcgccgct ctcaacgtac gactcgatgc gatacctgat cacaccgatc
     5521 gtcagtacgt ttgatcgacc gtcaggaaaa gtctgtgtct cggttacttc agcgacacaa
     5581 ccaacatgac cttttggtgg cacttcattc tcggaagttg ttggatcgaa gtaagagaga
     5641 ccaaaaaggt tcttcccgag gcggatgtcg agaagcattt gcctgtagcg cggctcgaag
     5701 atgtgcaaag gcagaggcac acccggaaac aacactactg gcaggggaaa gagtgggagc
     5761 tcgcgctgcc caagcacttt atcgctggct tcgctcattt aatttatcgg ctacggagag
     5821 gattatggca gagggcgcgc agggccgcaa agaggtaaaa aaaattcggc ccccgctatt
     5881 cgtgttgggc aacgtgagcg ggggccatcg agcacaccgt cagatgaaga ggggttccat
     5941 ccacggtgct gtacggacgc caaagggagt gtgcgtccgg atgtcaaaga atctgcgcga
     6001 acagttccag cctatgaacg ggggtcaacc ggcaaatgct cgccagcatg tcttcaagct
     6061 caaagggttt ggcgaaaaaa ccgtctgcgc cggcgttgac gcacgccttc cgcacctcgg
     6121 ggtcagtgtt cgagctcatg atcaaaacct tcgagttcgc gaggtgtcct ttgatgtact
     6181 caatcaaccg cagacccatg gcgggcgagt ctttatcggg aggcatttgc aagtccaata
     6241 tcgagatatc gggcttgtag aacgtcgccc gccgaatcgc ggtctgcata tcgttggcgg
     6301 tcaccacgtc gtactcatca cacaaagtcc agtagagctg ttgtgtgatc tgtgaatcgt
     6361 cgtcgactat caaaatcctg ggcttcatta cggggagttg tggagcaagg gccgtaccgc
     6421 gtcatcgatc aacgtacgtg cgctgtaaac aaagctgatg cgagatttgc agggaatttt
     6481 gcgtgtagac ggatgggcac agtgaggcac tctggcgtcc tcatttgtag actattttag
     6541 ccgcggattt tcgcggataa acgcggatca gaataagaaa tgttt
//
LOCUS       DASSFQ010000153         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2010425, whole genome shotgun sequence.
ACCESSION   DASSFQ010000153 DASSFQ010000000
VERSION     DASSFQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010425"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFH96_05320"
     CDS             <1..460
                     /locus_tag="VFH96_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973422.1"
                     /translation="AVKLGWLAPSGRFDWSDIILPAFTLGAALSAILTRMVRSSVIEE
                     LGEDYVRTARAKGLSERTVVYKHVLKNGLIPVVTILGLQLGVLLAGAIITEKIFGWPG
                     LGLLLVEDGIGKRDYRVVQGCVLVISMTYIIANLLTDMVYRWLDPRIRVG"
     gene            498..1313
                     /locus_tag="VFH96_05325"
     CDS             498..1313
                     /locus_tag="VFH96_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973423.1"
                     /translation="MNRLTIIGLVIVVVLVLVALFAPWIATYDVGATDLTMRYLPPSA
                     AHWFGTDSTGRDIFSRVVFGARISLQVGLVVVAVSAVIGTLLGAFAGYYGGWVDRVVS
                     GYVFNVFLAFPGLLLAIAMVAFLGAGLNKLILALCIIGWVGYARLIRGQVLKVREYDF
                     VQAARALGASDARILLVHILPNAIQPLIVQASLGMAGAVLSEASLSFLGLGVPPPAPS
                     WGVMIEEARDLSTLQAAPHALIFPGIAIALTVLAFNFIGDGLREYLDPRQRAR"
     gene            1310..2239
                     /locus_tag="VFH96_05330"
     CDS             1310..2239
                     /locus_tag="VFH96_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973424.1"
                     /translation="MSAISIRNLSKTYPVPFRRVRAFFRRPVKDPVEALRDVSFDVET
                     GEIFGLIGRNGAGKTTLTKIVATLVQPTTGSVSVHGHDSVSNDEHVRRQIGLSTAEER
                     SFYWRLTSEQNLMFFARLHGLSDRVAKQRIKDVFTKLELDEVARRRFGELSTGNKQRL
                     AVARALLAIPPVLLLDEPTRSLDPLAAARMRHLIRSLANQDPPVTILLTSHNLTEVET
                     LCARVAIISRGRIRAIDTPRNLRDLTSGSETVEVTVASGEKFSFTRQHDDDLLDQKIR
                     ELQKLGKRIIDIETKRPTLLDVLERFEAADERE"
     gene            2226..3071
                     /locus_tag="VFH96_05335"
     CDS             2226..3071
                     /locus_tag="VFH96_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973425.1"
                     /translation="MNANNMKNLTLLPRRIFAFVARDFRLFVSYRMQFFLRILSVLTV
                     VTTLFFISKIFAGFTDPQFTQWRDPLASWLTGLAVLNYFMTGFSSLANSIRQEQMQGT
                     LESVLLTPINLPTIIVASSAWDFLETTFFSFLYLFFGRVFFGVQFRGSFFLALVFLLL
                     TTVVLACLGILSASFAMVFKRGDPFGIFLGAGSALFSGVFFPTQLLRDYAGPKLGTIS
                     LILPATHGLQGIRQVLIEGHSLAQVTQPLFTLLGFLVVLLPFSLWVFGRAVRRAKREG
                     SLIQY"
     gene            complement(3068..3454)
                     /locus_tag="VFH96_05340"
     CDS             complement(3068..3454)
                     /locus_tag="VFH96_05340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET6973426.1"
                     /translation="MITAQVRRQIRKRAHNLCEYCHLHQDDSPLAALHVEHIIPKVHG
                     GTDDIENLALACIDCNLHKGTNLTEIDPQTSELTELFHPRRQNWDEHFELHGVYLVGK
                     TAIGRTTIRVLGMNSDDQIALRSSLG"
     gene            complement(3435..>3700)
                     /locus_tag="VFH96_05345"
     CDS             complement(3435..>3700)
                     /locus_tag="VFH96_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973427.1"
                     /translation="ETLAFDRGVRPVMEIVLDKADAIIHFRADPQLEARIEELARKST
                     EGELTDDERAEYAGYVRANKFVAILKRQAQHLTSLQPDDHRTS"
BASE COUNT          750 a         1089 c          954 g          907 t
ORIGIN      
        1 cgctgtaaaa ctcggctggc tcgcaccgtc aggacgtttc gactggagcg acatcatcct
       61 tcccgcattc acgctcggcg ccgcactatc tgccatcttg acgcgcatgg tgcggtcgtc
      121 tgtgattgaa gagctcggcg aagattatgt tcgcacagcg cgagcgaaag gtttgagcga
      181 acgaacagtc gtttataaac acgtattgaa gaacggcttg attcccgtcg tcacgattct
      241 tggtttgcag ctcggcgtcc tgctcgccgg cgcaatcatc accgaaaaga tcttcggctg
      301 gcctggcctt ggcttgcttc tcgttgagga cggcatcggg aaacgcgact atcgcgtcgt
      361 gcagggttgc gtgctcgtga tcagcatgac ttacatcatc gcaaacctgc tcacagacat
      421 ggtctaccgc tggttagatc cgcggattcg agtcgggtaa tccgtctttt atccgtgtaa
      481 tctgtggcta aaaacagatg aatcgtctaa ctatcattgg attagttatc gtcgtcgtgc
      541 tcgtgctcgt cgcactcttc gcgccgtgga tcgcaacgta tgacgtcggt gcgactgatc
      601 taacgatgcg ttacctgccg ccatccgctg cgcactggtt cggcaccgac tcgaccggcc
      661 gcgatatctt ttcccgcgtc gtatttggcg cacgcatctc cttgcaagtc ggacttgtgg
      721 tggtcgctgt ctctgccgtc atcggcacgc tgctcggcgc gttcgcgggt tactacggcg
      781 gctgggtcga tcgcgtcgtc tcaggttacg tcttcaacgt cttcctcgcg tttcccggac
      841 tgctgctggc aatcgcaatg gtcgcattcc tcggcgcagg cttgaataaa ctcattctcg
      901 ctctctgcat catcggctgg gttggttacg cgcgtctcat tcgaggccag gtattgaaag
      961 tgcgtgaata tgacttcgtc caggcagcac gcgcactcgg cgctagcgac gcacgcatcc
     1021 tgctggtcca catcctgccg aacgcgattc aaccattgat cgtgcaggcg agtttgggaa
     1081 tggccggcgc tgttttatcc gaggcgtcgt tatcattcct cggcctcggc gtgccgccac
     1141 ccgcgccgtc gtggggagta atgattgaag aagctcgcga tctatcaaca ctgcaagccg
     1201 cgccacacgc gttgatcttc cccggcatcg ccatcgcact cacagtgctg gcgttcaact
     1261 tcatcggtga tggcctgcgc gagtacctcg atccccgaca acgagctcga tgagcgccat
     1321 ctcgatccgc aatctctcga aaacttatcc cgtgcctttc agacgagtgc gcgcgttctt
     1381 tcgccggccc gtgaaagatc cagtcgaagc gctgcgcgac gtttcgtttg acgttgaaac
     1441 tggtgagatc ttcggattga ttggacgcaa cggcgcgggc aaaacaacgc tgacgaagat
     1501 cgttgccacg ctcgtccaac cgacaaccgg cagcgtctca gtgcacggtc acgacagcgt
     1561 cagcaacgac gagcacgttc gacgccagat cggattatcg accgctgaag aacgaagctt
     1621 ttactggcgt ttgacgtccg agcagaatct gatgtttttc gcgcgcctgc acggactttc
     1681 tgatcgggtc gcgaagcaac gtatcaaaga tgtatttacg aagctcgagt tggacgaagt
     1741 ggcgcgtcgt cgctttggcg agctgtcaac tggcaacaag caacggctgg cagtcgcacg
     1801 tgcattactc gcaatcccgc cagtgctttt gcttgatgag ccgacgcgtt cactcgatcc
     1861 gttggcggcg gcgagaatga gacacttgat ccgctcgctc gctaaccagg atccaccggt
     1921 cacgattctg ctcacgtcac acaatcttac tgaagtcgag acgctctgcg cgcgcgtggc
     1981 aataatcagt cgcgggcgca ttcgcgccat cgacacacca cgcaacctcc gagacctgac
     2041 ttccggatct gagacggtgg aagtaactgt tgcgagcggc gaaaagttct cgttcacacg
     2101 acaacacgac gacgatctcc tggatcagaa aatccgagag ttgcagaagc taggcaaacg
     2161 aattattgac atcgaaacca aacgaccaac gctgctcgat gtcttagaaa gatttgaagc
     2221 cgcggatgaa cgcgaataac atgaagaatc tcacgctact tcctcgtcga atcttcgcct
     2281 tcgtggctcg cgacttccgc ttgttcgtga gctatcgcat gcagttcttc ctgcgcatac
     2341 tctcggtact gacagttgtc acaacactct tcttcatctc aaagatcttc gccggcttta
     2401 ccgatccaca attcacacaa tggcgcgacc cgctcgcctc atggctcaca ggcctcgccg
     2461 ttctcaacta cttcatgacc ggcttctcca gtctcgcgaa ttcgatcagg caggaacaaa
     2521 tgcagggcac cttggagagt gtgctactaa cgccgatcaa cctgccgacg atcatcgtcg
     2581 ccagctcggc gtgggatttt ctcgagacga cgttcttctc gtttctttat ctcttcttcg
     2641 gtcgagtgtt cttcggcgtt cagtttcgcg gcagcttttt tctcgctcta gtttttcttt
     2701 tactaacgac tgtagtgctc gcatgtttag gaatactctc ggcgagtttc gcgatggtct
     2761 tcaagcgcgg tgatccattc ggaatatttc tcggagccgg atcagcatta ttttccggag
     2821 tcttcttccc aacccaacta ttaagagact acgccggtcc aaaactcggc accatctccc
     2881 tcatcctccc cgccacccac ggcctccagg gcatccgcca ggtcctgatc gaaggccaca
     2941 gcctcgctca agtcacccag ccattattca cattattagg attcctggtc gtcctacttc
     3001 ccttctccct atgggtcttc ggccgcgccg tccgtcgcgc caaacgcgaa ggaagcttga
     3061 tccagtatta gcctaaagac gaacgtagcg cgatctgatc atcggaattc atacctaata
     3121 cgcgaatggt agtcctgccg atcgccgtct ttcctactaa atagactccg tgcaactcga
     3181 aatgttcgtc ccagttttgt cgccgcgggt ggaacaattc ggttaactca ctagtctgag
     3241 gatcgatttc cgtcaggttc gtgcctttgt gcagattgca atcgatgcag gcgagcgcga
     3301 ggttctcgat atcgtcagtc ccgccatgga ccttcggaat aatgtgctca acgtgcaacg
     3361 ccgcgagcgg agagtcatcc tgatgcaagt gacaatactc gcaaaggttg tgagcgcgtt
     3421 ttcgaatttg tcttctaact tgtgcggtga tcatcgggct gcaaactggt gaggtgttgc
     3481 gcctgccgtt ttagaatggc gacaaactta ttcgctcgaa cgtaacccgc gtactcggcg
     3541 cgctcgtcgt ctgtaagctc gccttccgtt gatttccgcg ccaattcttc tatgcgcgcc
     3601 tcaagctgtg gatcggcgcg aaaatggatg atggcatcag ctttatccag gacaatctcc
     3661 atcaccggcc gcacgccacg atcaaatgcc agagtttctg
//
LOCUS       DASSFQ010000154         5037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2011612, whole genome shotgun sequence.
ACCESSION   DASSFQ010000154 DASSFQ010000000
VERSION     DASSFQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011612"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1488
                     /locus_tag="VFH96_05350"
     CDS             181..1488
                     /locus_tag="VFH96_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6973428.1"
                     /translation="MAVGTAGRRFLRRGARGVRRTFSRRSRLLAYLAILGPGMIAANA
                     GNDAGGIATYASAGADYGYTILWVLIPITISVGMVQEMCARMGAVTGKGLADLIRERF
                     GVRWTALIMLALLVANAGVTVSEFVGIAAASELFGLSHYISLPLVAILVWFLIVKGSY
                     KRVERVFLLMSLVFLGYIVSAFLARPDWGAVAVGLVRPEFKLEPAFLFTFVAVIGTTI
                     SPYMQVYVQSSVVEKGVTTDDYSMTKLDVWVGTIFAILIVFFIVVSTAATLHKAGIQV
                     TSAADAAHALRPLAGRYAQTLFGIGLLGASMLAAGVLPLATAYSISEALGFEKGVSRS
                     FREAPIFLGTFTFLIAVGAAIAIVPNLPLIRVLLVTQVINGLLLPVVLFAVLKLVNDR
                     ELMGRHVNGPLYNIAAWLTAILVTILSLLFILISLFPALFGHK"
     gene            1504..2949
                     /locus_tag="VFH96_05355"
     CDS             1504..2949
                     /locus_tag="VFH96_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6973429.1"
                     /translation="MARPRSTNKLILFLAASAAALVAAYLFWSLAADFKALLEHRMGA
                     KPSPSADTIVKTIKLLFIGIIGYLFVRALNSLFFGLAFRLKRIDAPTLIRNIFTIVTF
                     TILFLIAFMFLFPDQNLGALFTTSAIFGVILGLALQDTLGNFFAGISLQADRPFQVGD
                     VITVGAQRHTGVVEEISWRAIKIRTFTNHVVLIANSNAAKEPIEVCPRDNLNARLVLF
                     NTLYTDSPAKTIHVVREAVREADNVSDKVAPIVRIRGLGDNGVDYEVKYWLVDYAKYN
                     DTDALVRQRIWYAFRRAGLNFAFPTRTLYVERPERAAQRDGDGGAVVERLSAVDIFAP
                     LSADETSMLAQAAVRHVFAPGETVIRAGDPGSSMFVVHKGRVRVQVSENGRPRTVATL
                     NEGDFFGEMALFTGEPRTANVLALEETEVLEIGHAAMKRVFDTNPDLVESLSFIMAER
                     RQGLASQTDDSAANTHSSAGILSAIKRFFGI"
     gene            3157..3678
                     /locus_tag="VFH96_05360"
     CDS             3157..3678
                     /locus_tag="VFH96_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6973430.1"
                     /translation="MTVVRYDPFRDLRTLQEEVNRLFSTNLTRSFGDEGIGRGAWAPS
                     VDIYENKDQIVLEAELPGMKQEDFDLSIENNVITLRGERKFEKTDETDNYHRVERSYG
                     AFTRSFTLPQTVSAEGATAEYNNGVLRVTLPKREETKSRRIQVTGGSAGATQKTIDTT
                     AETGKAKEASGGR"
     gene            3805..>5037
                     /locus_tag="VFH96_05365"
     CDS             3805..>5037
                     /locus_tag="VFH96_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET6973431.1"
                     /translation="MRLDKFTLRGQEAVQSAIELAERNQHQQVEPEHLLVVMLEQPEG
                     IVRPILGKLGANVAVILNDTQAAVARFPRVQGGQQYFSSRITQLFAAAQKQADQMQDE
                     FISTEHLLLAIVEEKDGDAGKILRQHGVTRDDLFKVIEQMRGGSRITDQNAEQNYQAL
                     SKYARDLTEAARKGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVSGDVPETLRNKKLVALDLGSMLAGAKYRGEFEDRLKSVLKEIENAQGAIILFI
                     DELHTLVGAGAAEGAIDASNMLKPALARGELRCVGATTLAEYQKHIEKDAALERRFQQ
                     VYVGEPSVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSNRYITDRFLPDKAIDL
                     IDEAASRLRIEIDSLPQEI"
BASE COUNT         1065 a         1415 c         1427 g         1130 t
ORIGIN      
        1 aatcacctca cagttccctg tttctcaact cgttttcgtg ctaccggaca gttttcacac
       61 tattaagttt gtacctttcg gattttctgt ttaatctaag cgtttggtta ctcagttccg
      121 tttgaagtcc caattgataa tcgcgaatga ccgaggaccc tccaaatggc cgattcccgc
      181 atggcagttg gaactgctgg acgccgcttt ctgcggcgcg gggctcgggg tgtacgcaga
      241 accttctctc gtcgttcccg ccttctagct tacctggcca tcctcgggcc gggtatgatc
      301 gcggccaacg ccggtaacga cgccggcggg attgctacct acgcctcggc cggtgccgac
      361 tatggctaca caattctctg ggttctgatc ccgataacaa tcagtgtggg aatggtgcag
      421 gagatgtgcg ctcgcatggg cgcggtcacc ggcaagggtc tcgccgatct gatccgcgag
      481 cgctttggtg tgcgctggac ggcgctgatc atgctcgcgc tgctggtcgc gaatgcaggg
      541 gtgacggttt cggagttcgt cggcatcgcc gcggcgtctg agctgtttgg cctgtcacac
      601 tacatctctt tgccgctcgt tgcgattctc gtctggtttc tcatcgtgaa gggttcttat
      661 aaaagagtcg agcgggtgtt tttattaatg tcgctcgtgt ttctcggcta catcgtttcc
      721 gcgtttctgg cgcgaccgga ttggggcgcc gtggcggtgg ggcttgtccg tccggagttc
      781 aagctcgaac ctgcatttct attcacgttc gtggccgtga tcggaacgac gatctcgccg
      841 tacatgcagg tctacgttca gtcgtcggta gtcgaaaaag gagtgacgac tgacgactat
      901 tcaatgacga aactggacgt gtgggtcgga acgatcttcg cgatcctgat cgtgttcttc
      961 atcgtcgtct caaccgcagc gacgcttcac aaagcaggta tccaggtaac gagcgcggcc
     1021 gacgccgctc acgccctgcg accactcgcc ggacgttacg cgcaaacgct gtttggtatc
     1081 ggactgctcg gagcgtcgat gttggcggcg ggcgtgttgc cgctcgcgac ggcgtattcg
     1141 atcagcgagg cactcgggtt tgaaaaaggc gtctcgcgga gttttcgtga agcgccgatc
     1201 ttcctcggca cgtttacttt tctgatcgct gtcggcgccg cgatcgcgat cgtgccgaac
     1261 ctgccgctaa tccgcgtcct gctcgtgacg caggtgatca acggcttgct gttgccggtg
     1321 gtgctgtttg cggtgctgaa actcgtgaac gatcgcgagc tgatgggccg ccacgtaaac
     1381 gggccgctct ataatattgc cgcgtggctc accgcaattc tcgtgacgat tctctcgctc
     1441 ctctttatac tgatcagtct gtttccggcg ttgtttggcc acaaatgaac acggatgaca
     1501 cggatggctc gtcctcgttc tactaacaag ctcatactct tcctcgcggc ctcggcagcc
     1561 gcgctggtcg cagcttacct gttctggtcg cttgctgctg atttcaaagc cctgctcgag
     1621 caccggatgg gcgcgaagcc ctcgccatca gcggacacga tcgtcaaaac aatcaagctg
     1681 ctcttcatcg ggatcatcgg ctacctgttt gtccgcgcgc tcaattcact ctttttcggc
     1741 ctcgccttca gactgaaacg aatcgacgcg cccacgctca ttcgcaatat cttcacgatc
     1801 gtcacgttca cgattctctt tctgatcgcg ttcatgtttc tctttccgga tcagaatctc
     1861 ggcgcgctgt tcacgacttc cgcgatcttc ggcgtgatcc tcggacttgc gctccaggac
     1921 acgctgggca acttcttcgc cggaatttcc ctgcaggctg accggccgtt tcaggtcggt
     1981 gacgtgatca cggtgggcgc gcaacggcac acgggcgtgg tcgaagagat cagttggcgc
     2041 gctataaaga taaggacgtt cactaaccac gtcgttctga tcgccaatag taatgcggct
     2101 aaggaaccga tcgaggtttg cccgcgtgat aacctgaacg cgcgacttgt cctctttaat
     2161 actctctaca cggattctcc tgcgaagacg attcacgtcg tgcgcgaggc agtgcgcgaa
     2221 gcggataacg tgtcagacaa ggttgcaccc atagtccgca tcaggggcct cggcgataac
     2281 ggtgtcgatt acgaggtgaa atactggctg gtggactacg ccaagtacaa cgacacagac
     2341 gcactcgtcc ggcaaaggat ctggtacgcg ttccgccgcg ccggcttgaa cttcgcgttt
     2401 cccacgcgaa cgctatacgt cgagaggcct gagcgcgcgg ctcaaaggga tggcgatggc
     2461 ggcgcggttg tggagcggct gtcggcagtc gatatttttg cgcccctttc ggcggacgag
     2521 acctcgatgc tggcgcaggc cgcagtcaga cacgtgtttg cgcccggcga gactgtgatt
     2581 cgggccggcg acccgggatc gtcgatgttt gttgttcata aaggacgggt gcgggtccag
     2641 gttagcgaaa atggtcgtcc gcggaccgtc gcgaccttga atgaaggcga cttcttcggc
     2701 gagatggcct tatttaccgg cgagccgagg acggcgaatg tgctcgcgct cgaagagact
     2761 gaggtcctcg aaatcggcca tgccgcgatg aagcgggtat tcgatacaaa tccggacctg
     2821 gtcgaatcgc tcagtttcat catggccgaa cgccgccagg gcctcgcctc ccaaaccgac
     2881 gactccgctg ccaacaccca ctcctccgcc ggcatcctct ccgctattaa acgcttcttc
     2941 ggtatttaag ccacaaaagg cacaaaaagc acaaaagaaa acctttatat aagtaagacg
     3001 cccgcctgat tcagaagcta ttttgtgctt tttgtgcctt ttgtggctta aatacatgac
     3061 ttgacagctc gggactcaac ctttattatg acgctacgct cagctactag ccgggcttac
     3121 acaacaagga ataataggag gaggagaagt tagaccatga ctgttgttcg ctatgacccg
     3181 ttccgcgacc tgcggacgtt acaggaagaa gtgaatcgcc ttttctcaac caaccttacc
     3241 cgctcgttcg gcgacgaggg cattggccgt ggcgcttggg cgcccagcgt tgacatctat
     3301 gagaacaagg accagatcgt gctcgaggcc gagcttcctg gaatgaaaca ggaagatttc
     3361 gatctctcca ttgagaacaa tgtcattacc ctccgcggtg agcgcaagtt tgaaaagacg
     3421 gatgagactg acaactacca tcgagtcgaa cgctcgtatg gcgccttcac acgttctttc
     3481 actttgccgc aaacagtttc cgctgaggga gcaactgccg agtacaacaa cggcgtgttg
     3541 cgcgtgacgt tgccgaaacg agaagagacg aagtcccgac gtattcaggt gaccggagga
     3601 agcgccggag ccacgcagaa gacgatcgac acgaccgccg aaaccggaaa agcgaaagaa
     3661 gccagtggcg ggcgctaaag ctttgaggga tcggtaaaac gcgagcgggc tcgtcgcagt
     3721 ttgggatgcg aatgtcgagc ccgttcgatt tcttttagag agttcacgac agaggtagtt
     3781 ttgtaaaaga cagacgtaat catcatgcgt ttagataagt tcacactgcg cggacaggaa
     3841 gcagtccaat ccgctatcga gctggcggag cgtaaccagc accagcaggt tgagccggag
     3901 catctgctcg tcgtcatgct cgaacaaccg gaaggtattg ttagaccaat actcggcaag
     3961 ctcggcgcca acgtcgctgt cattcttaac gacacgcagg cggccgtggc tcgctttcca
     4021 cgtgttcagg gcgggcagca atacttcagc tcgcgcataa cccaactctt tgcagcggcg
     4081 caaaagcaag cggaccagat gcaggacgag ttcatctcga ccgagcacct gctgctggcg
     4141 atcgttgaag agaaagacgg cgacgcgggt aagatcctgc gccagcatgg cgtcacgcga
     4201 gacgatctgt tcaaagtaat cgaacagatg cgtggcggct cgcggatcac cgatcagaac
     4261 gccgaacaaa actatcaggc actttcgaaa tacgcgcgcg atctgaccga agccgcacgc
     4321 aaaggaaaac tcgatccggt tatcggtcgc gacgacgaga ttcgtcgcac gatccaggtc
     4381 ctttcgcgcc ggaccaagaa caatcctgta ttgattggcg aaccgggcgt cggtaagaca
     4441 gcgatcgtcg aaggtctcgc acagcgcatc gtctccggcg acgtgcctga aacactgcgt
     4501 aacaaaaaac tcgtcgcact cgatctcggc tcgatgctcg ccggtgcaaa gtatcgtggc
     4561 gagtttgaag atcgtttgaa atccgttctg aaagagattg agaacgctca aggcgcgatc
     4621 attcttttca tcgacgagct gcacacactc gtcggcgcgg gcgctgccga aggcgcgatc
     4681 gacgcgtcga acatgttgaa gcctgcgctt gctcgtggtg agctgcgctg cgtcggcgcg
     4741 accacactcg ccgaatacca aaagcacatt gagaaagatg cggcgctcga gcgtcgtttc
     4801 cagcaggttt acgttggtga gccgagtgtc gaagacacga tcgcgatcct gcgtggcttg
     4861 aaagagcgtt acgaagttca tcacggcgtt cgcatcaaag actcggccat cgtcgccgca
     4921 gccacacttt caaatcgtta catcacggat cgtttcctgc ctgacaaagc aatcgatcta
     4981 attgatgagg ctgcgtcgcg actaagaatc gagatcgatt cactaccgca ggagatc
//
LOCUS       DASSFQ010000155         3983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1846178, whole genome shotgun sequence.
ACCESSION   DASSFQ010000155 DASSFQ010000000
VERSION     DASSFQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846178"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VFH96_05370"
     CDS             <1..882
                     /locus_tag="VFH96_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6973432.1"
                     /translation="PAGLSTAFWCDELGLDALVLEQSEEVGGQLHRVYNPINNYPGLK
                     ARNGEELLERFTADVESADFDLWTQTSISSMDLKAKRVSLGSGEDLQSIALVIATGVR
                     PRELGVPGEKEFAGKGMIESATRDRELFAGQDVCVVGGGDAAVENALLLAEVCPTVTL
                     VHRGKKLRARPEFREKLQSQHCITVFTESVLTRIMGGDRVEAVEIQRKQGLKPFQLAV
                     RGLLIRIGVEPNTELFRQQLETENGYVVVNSQQETNVPMVFAVGDVSNPVAPTISGAT
                     GAGATAAKVIASRLTTK"
     gene            879..1295
                     /locus_tag="VFH96_05375"
     CDS             879..1295
                     /locus_tag="VFH96_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET6973433.1"
                     /translation="MTAYLDAHIAAYPIEYIAGIDLYNAGEFHAAHDAWEERWMGEVG
                     PKEKLFLQAMIQSAVAFHHLDIGRPGAARQMYLRAKEKFARLGVNVFMSLDLDEYQSQ
                     LDRALSWLLNGDPRELPRPQITPPKIRLLATINEFD"
     gene            1345..1815
                     /locus_tag="VFH96_05380"
     CDS             1345..1815
                     /locus_tag="VFH96_05380"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HET6973434.1"
                     /translation="MILLACWFGAALFFGAVVAPAAFGVLRSFGLPNANEIAGSIVTR
                     SLSVVNIAGFLISLLLLLTLLLRRNSSSRLSFIIECVCLGIIALTTGVGHWVIAARMR
                     ALRAAMVLPIDQIAADDARRIAFNNLHGYSVNALGLAMIAALVAMVLMARNLRN"
     gene            1827..2636
                     /locus_tag="VFH96_05385"
     CDS             1827..2636
                     /locus_tag="VFH96_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HET6973435.1"
                     /translation="MKLVEIIEDIDGWRRRFESWRPGAGDDLGADYPFVRNRRAPFTP
                     ARRALPMLNLALISSAGAYIDGTPAFDTNATNGDTTFKEIPIEVEESDLRFAARGYDP
                     SAVQQDVNSELPVGRLLEFEKNGIIGQLNPVYWSFCGFIPDAGRVVDELVPKLVERVR
                     RYEVQAALLVPASVLCHQSVGLVARGLELAGIPTMTLAVVKEIAESVRPPRVAFYDGE
                     PGTVSGLPNFPEHQRRILDEALRLIEPMDQPGIRKLVVALETAVENSRGER"
     gene            2647..3720
                     /locus_tag="VFH96_05390"
     CDS             2647..3720
                     /locus_tag="VFH96_05390"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6973436.1"
                     /translation="MSLKKRFGLVILLIIVVVLAYLFLAPVPIAPAAWTPPVAPALTG
                     QYAQNTRLSPVQKLSIGDGHKPEDVALDAEGKIYAGFEDGRIMVLQADGTQPRVFANT
                     HGRPLGMIFDREGNLIVADAIKGLLSVSKTGEINILAEEADGVKFGCLNDLDVGADGT
                     IYFTEASSKFPMSQFTNDLLEHQPNGRLLALDPNSRQPRTLLRGFYFANGVAVSPDQT
                     FVLVAETAEYRVRRVWLNGPKIGQSDIFIDNLPGFPDGISSNGRDRFWLALVTPRQAV
                     FDRMLPYPTMRKMVARLPKFMQPAPKRYSFVLGLDPQGRVIENLQNGSPDCFSEIANV
                     VERNGVLYLGSIGEDTVGRFSLK"
BASE COUNT          851 a         1065 c         1156 g          911 t
ORIGIN      
        1 ccagccggac tatcaaccgc tttctggtgc gatgagttgg gccttgacgc gcttgtactc
       61 gaacaatccg aagaagtcgg cgggcaactg caccgcgtct acaatccgat caataactat
      121 cctggtctta aggcccgcaa cggcgaggag ttgctcgaac gctttaccgc agatgtcgaa
      181 agcgctgact ttgatctgtg gacgcagaca agcatcagct cgatggattt aaaggcgaag
      241 cgtgtttcgc tgggtagtgg tgaagatctg cagtcaatcg cgctcgtgat cgcgactggc
      301 gtgcggccga gggagttggg cgtgcccggt gagaaagagt ttgctggtaa ggggatgata
      361 gaatccgcta cccgcgaccg agaactcttc gccggtcagg atgtttgtgt ggttggtggt
      421 ggagacgcgg cggttgagaa tgcgttgctg ctggcggagg tttgtccgac agtgacgctc
      481 gttcatcgtg gaaagaagct gcgcgcgcgg cccgagttca gggaaaagct tcaatcacag
      541 cattgcatca ctgttttcac tgaatctgtg ctcacgcgta tcatgggcgg tgatcgcgtc
      601 gaggcagttg agatccaacg caagcaggga ttgaagccgt ttcaacttgc cgtgcgcggc
      661 ttgttgatcc ggatcggagt agagccgaat accgagttgt tccgccagca gctggaaaca
      721 gaaaacggtt acgtcgtggt gaacagtcaa caagagacga atgtgccgat ggtgtttgcc
      781 gttggcgacg tctcgaatcc ggttgcgccg acaatcagcg gcgccaccgg agccggcgcg
      841 accgcagcga aagtgatcgc cagtcgactt actacaaaat gactgcgtat ctcgatgctc
      901 atattgcggc gtatccgatc gagtacattg ccggcatcga tctttacaac gcaggcgagt
      961 ttcatgcggc gcacgacgcg tgggaagaac ggtggatggg cgaggttgga ccaaaggaga
     1021 aactgttttt acaggccatg atccagtctg cggtcgcgtt tcatcatctc gacatcggcc
     1081 ggcccggcgc ggcgcgtcag atgtacttgc gagcgaagga gaagtttgcg cggttgggcg
     1141 tgaacgtctt tatgtcgttg gacctcgacg agtatcagtc acaactcgat cgtgcgttgt
     1201 cttggttgtt gaatggcgat ccgagagagt taccacgacc acagatcacg ccgccaaaga
     1261 tccgcttgct ggcgacgatt aatgaattcg attgacatct tgttaatcat gaaaatcctg
     1321 taatcctgta tgaatttctc acgcttgatt ttattagcat gctggtttgg cgcggcgctg
     1381 ttcttcggcg cagtcgtcgc acctgccgcc ttcggcgtct tgcgatcgtt cggccttccc
     1441 aacgcaaacg aaatcgccgg cagtatcgtc acgcgttcac tttcagtcgt aaacatcgcc
     1501 ggctttctaa tctcgttatt actactgcta acgttacttc tgcgccgcaa ttcgtccagc
     1561 cgtttgagtt tcatcattga atgcgtttgt ctcggcatca tcgcgttgac gaccggcgtc
     1621 ggccactggg taatcgcagc acggatgcgt gcgttgcgcg cggcgatggt tctgccgatc
     1681 gaccaaattg cggctgacga cgcgcgccgc atcgctttca acaacctgca cggttattcg
     1741 gtgaacgcgc tcggacttgc tatgattgcc gctctcgtcg cgatggtcct gatggctcgg
     1801 aacctgcgca actaaatctc agatggatga agctggtgga aattattgaa gacattgatg
     1861 gttggcgaag acggttcgag agttggagac cgggcgccgg tgatgatctc ggtgcggatt
     1921 atccttttgt aagaaaccgg cgcgcgccat tcacgcccgc acggcgcgca ctgccgatgc
     1981 tgaatctcgc gctgatctct tcagcgggtg cttacatcga tggcactccc gcgttcgaca
     2041 cgaacgcgac gaacggtgac accactttca aagaaatccc gatcgaagtt gaagagtcgg
     2101 atctgcgctt tgccgcgcgc ggctacgacc cgtcggctgt gcagcaggac gtgaactccg
     2161 aattgccggt tgggcgactg ctcgaatttg aaaagaacgg aatcatcggg cagttgaatc
     2221 cggtgtactg gagtttttgc ggattcattc ctgacgcggg tcgtgttgtc gacgagctcg
     2281 ttcctaagct tgtcgagcga gtgcgacgtt atgaagtgca ggctgcgctt ttagttcctg
     2341 cttctgtttt gtgtcaccag tcggtgggcc tggtggcgcg gggactcgag ctcgcgggca
     2401 ttcccacgat gacgcttgca gtggtgaaag aaatagccga gtcagtgcgt ccgccgcgtg
     2461 tggcgttcta cgatggagag ccgggaacag tcagcggctt gccaaatttt ccggaacacc
     2521 agcgacgcat actcgacgaa gcactccgcc tgatcgagcc gatggaccag cccgggatac
     2581 gcaagctcgt agtggcgctg gaaactgcgg tcgagaactc acgaggcgag cgataacaaa
     2641 catttaatgt ctctcaaaaa gcgcttcgga ctcgtcatcc tcctcatcat cgttgtcgtg
     2701 cttgcatacc tttttcttgc cccagtgcca atcgcaccgg cggcgtggac tcctcctgtt
     2761 gcccctgcgt tgacaggcca gtacgcgcag aacacgcggc tctcgcccgt tcagaagctc
     2821 tcaatcggtg acgggcataa gccggaggac gtcgccctcg atgcggaggg caagatctac
     2881 gctggttttg aagacggccg gattatggtc cttcaagctg acggtacaca gccgcgcgtc
     2941 tttgctaaca cacatggtcg cccactcggg atgatcttcg atcgggaagg gaatctgatc
     3001 gtagcggacg cgattaaggg attgttatct gttagcaaaa caggcgagat caatatcctg
     3061 gcggaggaag cagacggcgt gaagtttggc tgtcttaacg acctcgatgt cggcgcagac
     3121 ggcaccatct atttcactga agcttcgagc aaatttccga tgtcgcagtt tacgaacgac
     3181 ttgctcgagc atcagcccaa cgggcgtttg ctcgcactcg atccaaactc tcgccagccg
     3241 cggacgttgt tgcgaggctt ttactttgcg aacggcgttg cggtgagtcc ggaccagacg
     3301 tttgtcctgg tggcggagac tgctgaatat cgcgttcgtc gcgtctggct taacgggccg
     3361 aagataggcc agagtgacat tttcatcgac aatcttccgg ggtttcctga cggcatttca
     3421 tcgaacggtc gcgatcgctt ttggctcgcg ctcgtaacgc cgcgccaagc tgttttcgac
     3481 cggatgcttc cttacccgac gatgcgcaag atggtggcgc gtcttcccaa attcatgcaa
     3541 ccggcgccca agcgttattc cttcgttctc ggtttagacc cgcaaggccg cgtcatagaa
     3601 aaccttcaga acggctcccc cgactgcttc tccgaaatcg ctaacgtcgt cgagcgcaac
     3661 ggcgtcctct acctcggcag catcggcgaa gacacggtcg gccgtttctc gctcaagtga
     3721 gcgtaatcga tccaacaata gccactttcg acaaagaata gccggaatcg acaaaaaata
     3781 gccggattcg catcttggca ggcgccacct gatgagctaa attgttcagt gcaggagcca
     3841 gagatgagta cctcaaaacc atcaaaatcg tcgaacgcct atagtgtaaa gtacagcaac
     3901 aaaaccggcg gtgcgtatgc tgacattaag gcagtcatac agagcgagcg cgagaactgg
     3961 acctcaacct caaataagac ttc
//
LOCUS       DASSFQ010000156         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_72757, whole genome shotgun sequence.
ACCESSION   DASSFQ010000156 DASSFQ010000000
VERSION     DASSFQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72757"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFH96_05395"
     CDS             complement(<1..740)
                     /locus_tag="VFH96_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HET6973437.1"
                     /translation="MTIKTFSLTLIILIAAIVTHAADVVQTANGPVEGRGVQPSGVRI
                     FRGIPFAQPPTGDLRWREPQPATSWKSVRQAVDFGPRCMQARIFDDMVFRSNGVSEDC
                     LYLNVWTPAKSARERLPVLVYFYGGGFVSGDGSEPRYDGESMAAKGIVVVSVNYRLGV
                     FGFMAHPELTKESAHSASGNYGLLDQTAALQWVQRNIASFGGDPTRVTIAGESAGSIS
                     VSAHMASPLSKNLIAGAIGESGAITGTLS"
     gene            812..>2614
                     /locus_tag="VFH96_05400"
     CDS             812..>2614
                     /locus_tag="VFH96_05400"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET6973438.1"
                     /translation="MMTRKWIVHKFGGTSVADAERYRAAAELVLARASDERVAVVVSA
                     MSGVTNGLIQAVDFAAARNESYPAKLQELQKRHLETIDKLSLTTTQTAVLRETIDGDF
                     KAIEEVLRGVWITRIPSERIMEFVAGHGELWSAQLLHAYLEANGHSATWLDARKVLVV
                     EPENNTVTIDWPLSQERVNEWVAEHGDKDFVVITGYVAATHDGSATTLKRNGSDLSAS
                     IFAALLDAASVTIWTDVDGVFSADPRRVPDAIVVPELSYQEAAELAYFGAKVIHPNTM
                     APAIAKNITVWIKNSFKPEATGTRISASSPPDLPIKGFAAVEDMALINVEGTGMIGVP
                     GVAHKLFGALRAVGVSIVMISQASSEHSICFAVPRAQAELARKTIEQTFYAELQRGEI
                     QTVDLRDDCCIVAMVGEGMIEKLGMAGQFFSALGKAGVNVRAIAQGSSERNISAVIEQ
                     HEATKALRALHSAFYLSSQTLSIGVIGAGLIGGTFLDQITSRVQELRNERGIDLRVRG
                     IMNSRKMLLHDRRIFLDGWRDELDASSAQADIEQFVAHVHAAHLPHAVIIDATASGEL
                     PKHYEGWVSRGINIITPNKQSNSGPLATYTSLRNA"
BASE COUNT          608 a          733 c          724 g          549 t
ORIGIN      
        1 gagagcgttc cagttatcgc accactctca ccaatcgcac cggcgatgag attcttcgac
       61 aacggcgaag ccatatgcgc gctgactgag atcgatcctg ccgactcacc ggcaatcgtg
      121 acgcgcgtag gatcgccgcc aaagctcgcg atgtttcgct gtacccattg caacgctgca
      181 gtctggtcca ataatccgta gttgcccgaa gcactgtgcg ccgactcttt cgttaactcc
      241 ggatgggcca taaatccaaa cactcccagc cggtagttaa cgctcacgac gacaatgcct
      301 tttgccgcca tgctctcgcc gtcgtagcga ggctcggatc cgtcgccgct cacgaagccg
      361 ccgccgtaga aataaaccag caccggcagt cgttcacgcg cggatttcgc gggtgtccaa
      421 acgttcagat agagacagtc ttcacttacg ccgtttgatc ggaagaccat gtcgtcgaag
      481 atgcgcgcct gcatgcagcg tgggccaaag tcgactgctt gtctcacgct cttccaactc
      541 gtagctggtt gtggttcgcg ccagcgcaga tcgccggtcg gcggctgcgc gaaggggatg
      601 cctctgaaga tccgaacacc tgacggttgc acaccgcggc cctcaacagg accgtttgcg
      661 gtttggacca cgtcagcggc atgagtaacg atggcagcaa tgagaattat gagcgtcagt
      721 gaaaaagttt taattgtcat atagaccatt tatacaggat catcgaacgt actgtacgtt
      781 ccgagttatt tgattcatct ggtagtctgt catgatgact cgcaaatgga ttgttcacaa
      841 gtttggtggc acgagtgtgg cggatgcgga gcgataccga gccgctgctg aactcgtatt
      901 ggcgcgtgcg tctgatgaac gcgtcgcagt tgtagtatcg gccatgagcg gcgtgacgaa
      961 tggtctcatt caggccgtcg acttcgctgc agctcgtaac gaatcttatc ccgctaaact
     1021 tcaggaactt cagaaaagac acctcgaaac aatcgataaa ctatcgctga ccactacgca
     1081 aaccgcggtg cttcgcgaaa cgatcgatgg cgacttcaaa gcgatcgaag aagtactgcg
     1141 cggcgtttgg atcacccgaa ttccttccga acggatcatg gaattcgtcg ccggtcacgg
     1201 tgagctttgg tcggcccaat tgctgcacgc ttatctcgaa gcaaatggac actcagcgac
     1261 gtggctcgac gcacgcaaag tactcgtagt cgaacccgag aacaacaccg tcaccatcga
     1321 ctggccgctg tcgcaggaaa gagtaaatga gtgggtcgca gaacacggcg ataaagactt
     1381 cgtcgtgatc accggctacg tcgctgccac ccatgacggc tcagcaacaa ccctgaaacg
     1441 aaacggcagc gatctctcgg cctcgatctt cgcggcgttg cttgatgccg cttcggtgac
     1501 catctggact gacgtcgacg gcgtgttcag tgcggacccg cgtcgtgttc cggacgcgat
     1561 cgtggtacct gagttgtcgt accaggaagc agcggagctt gcttacttcg gagcgaaggt
     1621 gatccacccg aacacgatgg cgccggcgat tgcgaagaac atcactgtct ggatcaaaaa
     1681 cagtttcaag ccggaagcta cgggaacaag gatctcagcc tcctcgccac ccgatctgcc
     1741 gatcaaaggc tttgcggcgg tcgaagacat ggcgctcata aacgtcgaag gcacgggcat
     1801 gattggcgtg ccgggtgtgg cccacaaact gttcggtgca ctgcgagcag tcggtgtctc
     1861 gatcgtgatg atctctcagg cgagctcaga acactcgatc tgcttcgccg tgccgcgtgc
     1921 gcaagctgag ctggcccgga aaacgattga gcaaacgttt tacgctgagt tgcagcgcgg
     1981 agaaattcag acggtcgact tgcgagacga ttgctgtatt gtcgcgatgg tcggcgaagg
     2041 aatgattgaa aaactgggca tggccggaca gtttttcagc gccctaggca aagcaggcgt
     2101 gaatgtgcgt gcaatcgcac aaggctcttc ggaacgtaac atctcagccg tcatcgaaca
     2161 gcacgaagcg accaaggcat tgcgggcgtt gcactccgcg ttttatctct ccagtcaaac
     2221 tctctcaatc ggcgtcattg gcgccggact aatcggtgga acatttttgg accagatcac
     2281 ttcgcgtgtt caggaattgc gcaacgaacg aggaatcgat ctgcgagtgc gcggcattat
     2341 gaacagccgc aaaatgctcc ttcacgacag gcgaattttt ctcgatggtt ggcgcgatga
     2401 gttggatgca agttctgctc aagcagacat cgagcaattc gtcgctcacg tccacgctgc
     2461 acacttgccg cacgcagtga tcatcgacgc cacggcaagc ggtgaactgc caaagcacta
     2521 tgaaggttgg gtgtcgcgcg gaatcaacat catcacgccc aacaaacaaa gcaactccgg
     2581 cccgctcgcg acttatacaa gcttgcgtaa cgca
//
LOCUS       DASSFQ010000157         7106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_74147, whole genome shotgun sequence.
ACCESSION   DASSFQ010000157 DASSFQ010000000
VERSION     DASSFQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74147"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2815)
                     /locus_tag="VFH96_05405"
     CDS             complement(<1..2815)
                     /locus_tag="VFH96_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET6973439.1"
                     /translation="MTVIPAVGLFLLISANPFAQSNQLPAPSSHLSDFAGVIDADTKT
                     RLESLLQGLKEKSKIDLYVAVVESTGAQDIDKFSQQLAREWNIGFKPNRTKTLLLVVS
                     TASKSSFTQFTRMVQTDLPDGVLGEMSYRMSGPLNDGRFAEAVDSGVHVFANALAEKI
                     GFKVSDIETSSTVAASVPTDSSQPVLVSAKDTPRTRPRVVRDEPKAEAQATPPIESPK
                     SEPTPSESPAAEPTESPKAEPTPSEESPKPDVVKTSKTEAKRPGGKKPPAAAPKSTPV
                     KQKTAAEIAEQDADESEEVELTLTKPLPERAVKLKEFLDTHPDSKARPRAIELLISTH
                     AGLGDQKLKNGDIAGGIEQMMRAIDEADTSISEKLFAGVIAQIPTNLYLRGEREAAYK
                     AAQSIETKFGNDPKRLLEMAGFYLSVERGSDTVRVAESAVKLAPDLAEAHRMLAVGLH
                     ISLRLDEAVAEYKKTLELDPTSKVSRGSLADLYRAAGKNEEALTLYNEQLAADPKDKA
                     ARAGKVIVLYELNRADEAGSALESALADEPRNLPLLSGAAYWLAAHNNNDKALELALK
                     ATAIEPRYTWAQIALAHALAGVKRPLDAERSLRYARQYGKFPTLTYELASVLASMGLY
                     EEAAEVLRESFTIKDDQIQAYLAGRVQVSDASFIDLLAPERRAGIYQPSSADTPTNAK
                     MMKALLAFSTAITPAEGQKIDETAAVAAAKEFASGSDSLRPFRQVYAASRLARNGVGI
                     NTALDLIAQARRATDEALKVPVLTLAVQADEFRDMRASAISSGSVPDIAPAPAPVLAN
                     IYKGRLEDLEGWALFNDEKYSEAVVHLKKAAEILPAETPAWRIALWHLGVTLEQSGQK
                     EQALDAYIKSYKGEPTPSAVRRSVIEQLYKRINGSLDGLDEKLGAATGTAVSSSTPAT
                     TTPETTTPAPQPTTTPEPAPVETP"
     gene            2919..3437
                     /locus_tag="VFH96_05410"
     CDS             2919..3437
                     /locus_tag="VFH96_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973440.1"
                     /translation="MSELDEAWAAALSEAERKARLAGRGEVAEYLSLKNSNDLLRKAG
                     IDWLLAEFTVLAGEANRAGASIQIARQEGHRFPTGTSTMVGHLLTLTNGVRTLYIEAG
                     WPRTPRDGIVRGGGLAAANIRHLGIKKANEELLLAKSQSGAPTWKSIEKNYLLLEADI
                     RRHLIILIDKRH"
     gene            3646..4236
                     /locus_tag="VFH96_05415"
     CDS             3646..4236
                     /locus_tag="VFH96_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973441.1"
                     /translation="MTNRHTLKFSIFAAAILALALPAAASAQYGRYPDDNRGYGRYDD
                     RGLKDSVQRLDRLAKDFERDMDRALDHSRANGSNREDQINNQVHQFRAAVGDLKSRVG
                     NGRDLNRSANEARRVLQEADDLDRFSRRGFDGRTASEWSQIQQELRYISSVYGFGYGG
                     GYGRDRDDDWRRNDRNDRNNNNRNNDWWRRIPFPRY"
     gene            4333..5175
                     /gene="rsmI"
                     /locus_tag="VFH96_05420"
     CDS             4333..5175
                     /gene="rsmI"
                     /locus_tag="VFH96_05420"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET6973442.1"
                     /translation="MPGTLYLVATPIGNLADITHRALQVLSDVDLIACEDTRHTHKLL
                     NHYGINTKTISYHEHNEQQRAAELIDRLKQGTSIAVVSDAGTPSISDPGFRLVRAAIE
                     NEISIVPVPGPSALIAALIAAGLPTDEFFFAGFLPSRTNARRARLTELQSVPGTLIFY
                     EAPHRLAATLKDAYEILGEREAVVARELTKLHEEIRRGLLSKLAADYTEKTDARGEIV
                     VLIDRNVIGAAVTEAVSVSMLVDQFERDGMDHRAALKKAARELGLPRAEAYRKLLSEK
                     SDPR"
     gene            complement(5194..6879)
                     /locus_tag="VFH96_05425"
     CDS             complement(5194..6879)
                     /locus_tag="VFH96_05425"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6973443.1"
                     /translation="MRRFLTSFVLASLLVTTVPASYAQRAAHKSTKKAAAPVAITKRG
                     VDTIAANQLRDYLTFIASDEMEGRDTPSRGLNTTAKFLAMNLTRWGFKPAGDEGSFFQ
                     RIDLRRERADTGQTKVDFSGRTLASGTDFLPLAGTGNVSGQLVFAGNGWFVKSKDIDA
                     YKGIDPAGRIAVIFGTPNMPPRGMSRADLRKPGEKIMDPIDYARSKGVVGLIYVPDDQ
                     FIRNWRRRQAVVERGSIAVAKFQPQTTTTPLPSIVITPDTANVIFEGESQTAAAISTA
                     TFGSNQGTVPAPFLLSDQKKITMNVANTSETIPTQNVVAVWEGSDPVLKAEYVALGAH
                     YDHVGSGCPPVGADTICNGADDDGSGTTALLGMAEALAKAPVRPKRSVLFVWHCGEEK
                     GLWGSRYFTEFPTVPLKQIVAQLNIDMIGRSKKDGDTNRKNIELTGPDAVYVIGSTMM
                     STELGALVNNVNNSFLKLTFDTKYDDPKDPHRFFYRSDHYNYARKGIPIIFFFDGEHE
                     DYHGAGDTADKIDYQKMEKITRTVYMTLWEIANRPARLKVDKPLPVQIGVSTD"
BASE COUNT         1456 a         2027 c         1974 g         1649 t
ORIGIN      
        1 tcggagtctc tacgggtgcg ggttcgggtg tcgtagttgg ttgcggcgca ggtgtggtcg
       61 tttcaggcgt tgtcgtggct ggcgttgaac tgctaaccgc tgttcctgtt gctgcgccaa
      121 gtttttcatc gagtccatca agcgagccgt tgatgcgctt gtaaagttgt tcgatcacgg
      181 agcgacgaac tgctgacggc gttggttcac ctttataact cttgatgtac gcatcgagcg
      241 cctgttcctt ttgccctgac tgttcgagcg tcacgccgag atgccagagt gcgatgcgcc
      301 atgcgggcgt ttctgccggc aagatctccg cggccttttt cagatggacg acggcctctg
      361 aatatttctc atcgttgaag agcgcccagc cttcgagatc ttctaaacgt cccttgtaaa
      421 tattcgcgag cactggcgcc ggcgcgggcg caatatcggg aacgcttcct gacgagatcg
      481 cactcgcgcg catgtcacga aattcatcgg cttgcaccgc aagggtgagc accggaactt
      541 tgagcgcttc atcagtcgct cgtctcgcct gcgcgatcag atcgagcgcg gtattgatac
      601 cgactccgtt acgcgccaat cgactcgcgg catacacctg cctgaatgga cgcaggctgt
      661 cgctgcccga ggcaaactct ttcgccgccg cgacggctgc cgtctcgtca atcttctgtc
      721 cctcagcggg cgtgattgca gtgctgaagg caagcaacgc cttcatcatc tttgcgttcg
      781 tgggagtgtc ggcggaactt ggctgataaa ttccggcgcg tcgttccggc gccaaaagat
      841 cgataaagct ggcgtcgctg acttgcacgc gcccggcgag gtaagcctga atctgatcgt
      901 cttttatcgt gaacgactcg cgcaaaactt cagccgcttc ttcatacaaa cccatcgacg
      961 ccagcacact cgcgagttca taagtaagcg tgggaaactt cccgtactgg cgcgcgtaac
     1021 gcagcgagcg ttcggcgtcg agcggacgct tcaccccggc aagcgcatgg gccaacgcga
     1081 tctgggccca cgtgtaacgc ggttcgatcg cagttgcttt gagcgccagc tcgagagcct
     1141 tgtcgttgtt gttgtgcgca gcgagccagt aggcagcgcc ggaaagtaac ggcagattgc
     1201 gtggctcgtc agcgagtgca gactcgagcg cgctgcccgc ttcatcagcg cgattgagct
     1261 catacagcac gatcactttg ccggcgcgcg cggccttgtc tttcggatcg gcggcgagtt
     1321 gttcgttgta gagtgtgagc gcttcttcgt tcttgccagc ggcgcgatag agatccgcga
     1381 gactaccgcg cgagactttc gacgtcggat ccagctcgag agtctttttg tactcagcca
     1441 cggcttcgtc gagtcgcaaa gagatgtgca acccgactgc gagcattcga tgtgcttcgg
     1501 cgagatcggg cgcgagcttc acggcacttt cggcgacgcg cacggtgtca ctacctcgtt
     1561 cgacgctgag gtaaaagccc gccatctcga gcagacgctt gggatcatta ccgaatttgg
     1621 tttcgatact ctgcgccgct ttgtaagccg cttcacgctc gccgcgcagg tataagttcg
     1681 tcggaatctg cgcgatcacg cctgcgaaaa gtttttcgga gatgctggta tcggcctcgt
     1741 cgatcgcgcg catcatctgc tcgatgccgc cggcgatgtc gccgtttttc aacttctgat
     1801 cgcctaagcc ggcgtgcgtg ctgatcagta attcgatggc gcgtggcctg gctttcgagt
     1861 caggatgcgt gtcaagaaat tctttcaact tcaccgcgcg ttccggtaac ggctttgtca
     1921 gtgtcagctc gacttcttct gactcatcgg cgtcttgttc tgcgatctct gcggcggttt
     1981 tctgtttgac gggagttgat tttggagcgg cggcaggtgg tttcttccct cctggtctct
     2041 ttgcttcagt cttcgatgtc tttacgacgt cgggctttgg cgattcctcg gatggtgtcg
     2101 gctcagcctt cggcgactca gtcggctccg ccgccggact ttcactcggc gtcggttcag
     2161 atttgggtga ttcaatcggc ggtgttgcct gtgcttcggc ttttggttca tcacgaacca
     2221 cgcgtggacg tgtgcgtggc gtgtctttcg cactgacaag gaccggttgc gacgaatcag
     2281 tcggaacgga cgctgcaact gtcgacgacg tctcgatatc ggaaactttg aaaccgatct
     2341 tttcagcaag cgcattcgca aacacgtgta caccgctatc aactgcttca gcgaaacggc
     2401 catcgttcag tggtccactc atgcgatagc tcatctcgcc cagcacgcca tccggcaggt
     2461 ctgtctgcac catgcgagta aattgcgtga acgacgattt cgaagctgtc gagacaacga
     2521 gaagcagggt cttcgtgcga ttcggcttaa agccgatatt ccactcgcgc gcgagctgtt
     2581 gcgagaattt atcaatgtcc tgagcgccgg tgctttcgac gacagcgacg taaagatcga
     2641 ttttgctctt ttccttgagg ccctgaagca ggctttccag ccgcgtcttg gtgtcggcat
     2701 cgatcactcc ggcaaagtcg ctaaggtgac tgctcggcgc aggcagttgg tttgactgtg
     2761 cgaacgggtt tgcgctgatt agcaaaaaca gcccaaccgc tgggattacg gtcaaaaggc
     2821 gcattagcgc tcgatgtgat cgggtcattg ttaatatgtt ccgcatctgg gattgtgggg
     2881 ggacgattcg gcgctcatga taccatcggc gagcgaatat gagcgaactt gatgaagcgt
     2941 gggcggcggc attgtcggaa gcggagcgca aggctcggct ggccgggcgc ggcgaggtgg
     3001 cggagtacct gtcgctgaag aattcgaacg acctgctgcg caaagcgggc atcgattggc
     3061 tgctcgcgga gttcacggtg ctcgcgggcg aagcgaatcg agcgggcgcg agtattcaga
     3121 ttgcgcgaca ggaagggcac cggtttccga ccgggacttc gacgatggtg ggacacctat
     3181 tgacgctcac aaatggtgtc cgcactttgt acattgaagc cggatggccg aggacacccc
     3241 gcgacggtat tgtgcgaggc gggggactcg cggccgcgaa tattcgtcac ctcggaatta
     3301 aaaaggcgaa tgaggagttg ctattagcca agtctcaaag tggtgcgccc acgtggaagt
     3361 cgatcgaaaa gaactacctc ctcctcgaag ccgacattcg gcggcatctg atcatcctca
     3421 tcgataaacg tcattagcca caaaaggcac aaaaagcaca aaagagattg agtcttcttt
     3481 tgtgcttttt gtgccttttg tggctaatga cgttttccgt caacctctgc accttttggc
     3541 actaatgacg cttaaacatt ctaattttgc ggcgatttcc acacaggcac agcgcttgcc
     3601 aaacgtccgg agcgacatcg gacttactga ggagaaattc aaaacatgac gaaccggcac
     3661 acactgaagt tttcaatatt cgctgcggcc attctggcgc tcgctctgcc tgccgcagcc
     3721 tctgcccagt acggacgcta ccccgacgac aatcgcggtt acggacgcta cgacgatcgc
     3781 ggccttaagg actccgtgca acgtctcgat cgtctcgcaa aagatttcga acgcgacatg
     3841 gaccgcgccc tcgatcacag ccgcgcaaac ggctcaaacc gcgaagatca gatcaacaac
     3901 caggtgcacc agtttcgtgc cgctgttggc gatctgaaat cgcgagtcgg caatggtcgc
     3961 gatctgaatc gtagtgcgaa cgaagcgcgc cgagtgctcc aggaagccga tgacctcgat
     4021 cgcttctcgc gtcgcggatt tgatggccgc actgcttcgg agtggtcgca gatccagcag
     4081 gagctgcgct acatcagcag cgtctacggt tttggctacg gtggtggtta cggccgcgat
     4141 cgagatgacg actggcgtcg caacgatcgc aacgaccgta ataacaacaa tcgcaacaac
     4201 gattggtgga gacgaattcc attccctcgg tattaacccg catctgactt ccgcctgtta
     4261 tctgctggtc taccctgcca gaaggcctcg gttcaaacgc cggggccttt atctttcctg
     4321 taaaatggtt tcatgcccgg caccctgtat ctcgtcgcca ctccgatcgg caatctcgca
     4381 gacatcacgc accgcgcgct tcaagttctc agcgacgtcg atctcatcgc ctgtgaagac
     4441 acgcgccaca cacacaagct cctcaatcac tacggtatca acacgaaaac gatcagctat
     4501 cacgaacaca acgaacagca acgcgcggcg gagttgatcg atcgactgaa acagggaacg
     4561 agcatcgcgg tagtttcaga cgcaggcacc ccgtcaatca gcgatcccgg ctttcgtctg
     4621 gtccgcgcgg caatcgagaa cgagatctcg attgttccag tgcctggtcc aagcgcgctg
     4681 atcgcggcac tgattgcagc cggtctcccg actgacgagt ttttcttcgc aggcttcctc
     4741 ccctcgcgta cgaacgcgcg ccgtgctcgg ctcaccgaat tgcaatcagt tcccggcacc
     4801 ttgatcttct acgaagcccc gcatcgcctc gccgccacgc tcaaagacgc ttacgagata
     4861 ctcggcgagc gtgaagccgt cgtcgcaaga gagctcacga agttacacga ggagatcagg
     4921 cgtggattgt tgagtaaatt agccgcggat tacacggaaa agacggatgc tcgcggagaa
     4981 atcgtggtgc tgatcgatcg caacgtgatc ggagctgctg tgactgaggc ggtgagtgtc
     5041 tcgatgttag tcgatcagtt cgagcgcgac ggcatggacc accgcgccgc tctaaaaaaa
     5101 gcggcgcgtg agcttggtct cccacgcgcc gaagcctatc gaaagctctt atccgaaaaa
     5161 tctgatccgc gttaatccgg ctcgaaaatc cgtctaatcc gtgctcaccc cgatctgcac
     5221 cggcagcggc ttatccacct tcaaccgcgc cggccggttc gcaatctccc acaacgtcat
     5281 atagaccgtc cgcgtaatct tctccatctt ctggtaatcg atcttgtcag ccgtatcgcc
     5341 cgcgccgtga taatcctcgt gctcaccatc gaagaagaaa ataatcggaa tccctttgcg
     5401 cgcgtagttg taatgatcgc tgcggtagaa aaaccggtgt ggatctttcg gatcgtcgta
     5461 cttcgtatca aacgtgagct ttaaaaacga attgtttaca ttgttgacga gtgcgccgag
     5521 ctccgtgctc atcatcgtcg agccgatcac ataaactgca tccggtccgg taagctcaat
     5581 gttctttcga ttcgtgtcgc catctttttt gctacgacca atcatatcga tattgagctg
     5641 cgccacgatc tgtttgagcg gtacggtcgg aaattccgtg aagtaacgcg atccccacaa
     5701 acccttctcc tcgccgcagt gccatacgaa cagcactgaa cgcttcggtc gcaccggcgc
     5761 cttcgcgagc gcttccgcca ttcccagcaa cgcggtcgtg ccggagccat cgtcgtccgc
     5821 gccgttgcaa atcgtgtctg cgccgaccgg cgggcaaccg ctacccacgt gatcgtaatg
     5881 cgcgccgagg gcaacgtatt cagccttcaa aaccggatcg ctaccttccc aaaccgccac
     5941 cacattctgc gtcggtatcg tctcggaggt gttagcgaca ttcatcgtga tcttcttttg
     6001 gtcgctgagc agaaatggtg cgggtaccgt gccctgattc gaaccgaaag tagcggttga
     6061 gattgccgca gcagtttgcg actcgccctc aaagatcacg tttgctgtgt ccggtgtgat
     6121 cacgatcgac ggcaacggcg tggttgttgt ttgcggttga aatttcgcga cagcgatcga
     6181 gccacgttcc acgacggcct gacgacggcg ccagtttctg atgaactgat cgtcaggcac
     6241 gtaaatcaaa ccgacaacgc ctttgctgcg agcgtaatcg atcggatcca tgatcttttc
     6301 cccgggcttg cggaggtcgg cgcgcgacat gccgcgcggt ggcatgtttg gagttccgaa
     6361 gatcaccgcg atacgtcccg ccggatcgat tcctttgtac gcgtcaatgt ctttcgactt
     6421 cacaaaccaa ccatttcccg caaacaccag ttgaccacta acattgccgg tgccggccag
     6481 cggcagaaaa tctgttcccg acgcgagtgt tcgcccgctg aaatcaacct ttgtttgacc
     6541 agtgtcagca cgttcacgtc gcagatcgat gcgttggaaa aagctgcctt cgtcgcccgc
     6601 cggtttgaaa ccccagcgcg tgagattcat tgcgagaaac ttcgccgtcg tgttgaggcc
     6661 gcgtgatggt gtgtcgcgtc cttccatttc gtcggaagcg atgaacgtca agtagtctct
     6721 gagttgattg gccgcgatcg tgtcaacgcc gcgtttagtt attgctaccg gcgctgcggc
     6781 tttctttgtt gatttatgag cagcgcgctg ggcgtagctt gcgggaaccg tggtgacgag
     6841 caaactcgcc aacacgaagg aggttaagaa tcttcgcatt tagtctccag aaaaatgatt
     6901 tcgaattatg taaatcctgt gaatcctgaa atcctgtcta agctgtttac aggattaaca
     6961 agatttacag gattacatga tgctaagcgt gctgcacggc ttgaaccagc cgagtcagcg
     7021 tgtctttcgc gtcgccatac agcatgcccg ttaccggctt aaaaaacaaa gggttctcca
     7081 aaccggagaa acctttgccc tgcccg
//
LOCUS       DASSFQ010000158         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1702512, whole genome shotgun sequence.
ACCESSION   DASSFQ010000158 DASSFQ010000000
VERSION     DASSFQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702512"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1996
                     /locus_tag="VFH96_05430"
     CDS             <1..1996
                     /locus_tag="VFH96_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973444.1"
                     /translation="ALSPHAVQCAKAEEVSKAMQEQDGKKQFNISERLEDLRREHEAL
                     RWEGTFRDYFELVAQNPRIAQLSHARINDMVHAAGIERLNEGTQHEVLSYNFFANELF
                     GIEEPIARIVEYFKSAAQRLEVRKRILLLMGPVGGGKSTIVNMLKRGLEDWTRTAEAG
                     AVYAIKDCPMHEEPLHLIPHQLRAEVEKHYGIYIEGDLCPQCRYALEHTYAGRHEDVK
                     VHRVAFSEKERVGIGTFSPSDPKSQDITELTGSIDLATIGEVGVESDPRAYRFDGELN
                     IANRGLMEFVEMLKVDEKFLYSLLTLSQEQSIKTGRFAMIYADEVILSHTNENEYIAF
                     AGNRKSEALQDRIILVRVPYNLRVSQEERIYDKLLHQSEALRNVHLAPHTLKVAAMFA
                     VMTRLEEPKRQNVDVVKKMKLYDGEDVEGYKSKDVRELKEETVREGMDGISPRYIINR
                     LSSALVRDGVTCINPIDALRAIKDGFEQHTGIGSEQREKYMNHISQARKEYDELAKIE
                     VQRAFVYSFEEMARTMCNNYLDNVEAFCNKERLKDPITEEEMEPDEQLMRSIEEQIGI
                     SENAKNTFRQEILIRISSYARKGKPFEYNSHERLKEAIEKKIFADLKDVVKITTSAKT
                     PDPDQLRRINEVVDRLVKEHSYCPVCANELLTYVGTLLSR"
     gene            2071..>2998
                     /locus_tag="VFH96_05435"
     CDS             2071..>2998
                     /locus_tag="VFH96_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6973445.1"
                     /translation="MKKRYWIAGAATVAIAGKLLLRPRDADWHKGRDVVFHSEHSCFI
                     DIDGVRVHYQEAGDRSAPAMVLIHGFASSTLVWSKVFLALANAGYRVIAPDLLGYGYS
                     AKPRNGEYTIEGQAKLLMGLLEALGIPRAIIVGSSYGGAVAATCALDYPERVEKLILV
                     GAVNNNRPLEFALMRLFGSPVFGDVVSPLLIGSRRLLRRRMKRVYDRHSWVLDERRVD
                     ARHLPLRAAGTHRAMIRTVRAWDAERISRDAHLISQPTLLLWGETDREIPISDAERLH
                     EEISGSRLIVFLNCGHLPHEEYPEEFTNVVIDF"
BASE COUNT          702 a          785 c          865 g          646 t
ORIGIN      
        1 ggccctctcc ccacacgctg tccaatgcgc gaaagccgaa gaggtttcaa aggctatgca
       61 agagcaggat ggcaagaaac aattcaatat cagcgaacgt ttggaagatc tgcgccgtga
      121 acacgaagcg ctgcgttggg aaggaacatt ccgagactac tttgagttag tcgctcagaa
      181 cccccgcatt gctcaacttt cacacgctcg catcaacgac atggttcacg ccgccggcat
      241 cgagcggctg aacgaaggca ctcagcacga agttctctcc tacaatttct ttgccaacga
      301 actgttcggc atcgaagagc ccatcgccag aattgtcgag tacttcaagt cggccgcaca
      361 gcgtctcgaa gtgcgcaagc gcatcctttt gttgatggga ccagtcggcg gcggcaagtc
      421 gacgatcgtc aacatgctca agcgtgggct ggaggattgg accaggaccg ccgaggccgg
      481 tgcagtttat gccatcaaag actgcccgat gcacgaggaa ccgcttcatc tcataccaca
      541 tcagttacgt gccgaagtcg agaagcatta cggaatttat attgaaggtg atctttgtcc
      601 gcagtgtcgc tacgcgttgg aacatactta tgcaggacgt cacgaggacg tcaaagtgca
      661 tcgcgttgct ttctctgaaa aggaacgcgt cggtatcggc acgttctcgc ccagcgatcc
      721 gaagtcgcag gacatcacgg agctgaccgg ctcgatcgat cttgcgacga tcggcgaagt
      781 cggcgtcgag tcagacccgc gcgcttatcg ttttgacggc gagttgaaca tcgcgaaccg
      841 cggcttgatg gaattcgtcg agatgctgaa ggtcgacgag aagtttctct acagcctgct
      901 gacgctttct caggaacaga gcatcaagac cggccgcttc gcgatgattt acgccgacga
      961 agttatcctc tcgcacacaa acgagaacga gtacatcgcc tttgccggca atcgcaaatc
     1021 ggaagcgttg caggatcgca ttattctcgt gagagtgcct tataacctgc gcgtttcgca
     1081 ggaagagcgt atttacgaca agctgttgca ccagtccgaa gccctgcgta acgtccatct
     1141 cgcgccgcac accttgaaag tcgccgcgat gtttgccgtg atgactcgtc ttgaagagcc
     1201 gaagcgtcag aacgtcgacg tcgtgaagaa gatgaagctt tacgacggcg aggacgtcga
     1261 gggttacaag tcgaaagacg tgcgcgagct gaaggaagag actgttcgcg aaggcatgga
     1321 cggcatctcg ccgcgctaca tcatcaatcg tctttcgagc gcgctcgtgc gtgacggcgt
     1381 cacttgtatt aatccgatcg atgcactgcg ggccatcaaa gatggttttg aacagcacac
     1441 cggcattggc agcgagcagc gtgaaaagta catgaatcac atctcgcaag cgcgcaagga
     1501 gtacgacgag ctcgcgaaga ttgaagtgca aagagcgttt gtctactcgt ttgaagagat
     1561 ggcgcgcacg atgtgcaaca actatctcga caatgtcgaa gcgttctgta acaaggagcg
     1621 actcaaggat ccaatcaccg aagaggagat ggaacccgac gagcagttga tgcgctcgat
     1681 cgaggaacag attggcatct cggaaaatgc aaagaatact ttccggcagg agattctgat
     1741 ccgcatctcg tcatacgctc gcaagggcaa gccgtttgag tacaactcac acgagcgttt
     1801 gaaagaagcg atcgagaaga agatcttcgc tgacctgaag gacgtcgtga agattaccac
     1861 ctcggcgaag acgcccgatc cggatcagct gcgacgcatc aacgaggttg tcgatcggct
     1921 tgtgaaagag cacagctatt gtccggtttg cgcgaacgag ttgctgacct atgtcggcac
     1981 gttgttgtcg cggtagaatt ttggccgcag atttacgcag atgaacgcag atctgatccg
     2041 cgttaatccg cgtgcatctg cggcttaaat atgaagaaac gatactggat cgcaggtgca
     2101 gcaacggtag ccattgccgg caagcttttg ctccggccac gtgacgccga ttggcacaag
     2161 ggtcgcgatg ttgttttcca ttctgaacac tcgtgcttca tcgatatcga cggtgtgcgg
     2221 gtgcattacc aggaagccgg cgaccgtagc gcgcccgcga tggtgctgat tcacgggttt
     2281 gcttcgtcga cgctcgtgtg gagcaaggtg tttttggcgc tcgccaatgc cggctatcgt
     2341 gttattgcac ccgatctgct cggttacggc tactccgcga agccacgcaa cggcgagtac
     2401 acgatcgaag gccaggcgaa actcttgatg ggactgctcg aggcgctcgg tattccgcga
     2461 gcgattattg tgggcagttc ttacggcggc gcagtcgcgg cgacttgtgc gctcgattac
     2521 ccagagcgag ttgaaaaact gattctcgtt ggggcagtca ataacaaccg gccgcttgag
     2581 tttgcgctga tgcgactgtt tgggtcgccg gtattcggcg atgtcgtttc gccactgcta
     2641 atcggatcgc gtcgtttgct gcgacggcgc atgaaacgag tttacgatcg acactcatgg
     2701 gtgctcgacg aacgacgagt ggatgctcgg cacttgcctt tgcgtgcggc gggcacgcat
     2761 cgcgcgatga ttcggacggt gcgcgcttgg gatgcagaac gcatctccag agacgcgcat
     2821 ctaatcagtc agcccacact gctcctctgg ggcgaaaccg atcgggagat accaatatcg
     2881 gatgccgagc gactacacga ggagatttcc ggatcgcgat tgattgtatt tttgaattgc
     2941 ggtcacctgc cgcacgaaga gtacccggaa gagttcacga atgtggtgat cgatttct
//
LOCUS       DASSFQ010000159         8980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_971324, whole genome shotgun sequence.
ACCESSION   DASSFQ010000159 DASSFQ010000000
VERSION     DASSFQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971324"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..3360
                     /locus_tag="VFH96_05440"
     CDS             109..3360
                     /locus_tag="VFH96_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973446.1"
                     /translation="MPAFLLLAVYGALSGVTQELAGYAGFDIAGFPHAEAFGLGSWIV
                     FGLLLVAMIGSFWERRSNAYLLGALAVLAAAIPLLAGRFESQIATATAWRGLAAMFLL
                     LGSIVIWSRRKIFGAGFLEEVRAVLFTLTILPLLILTSCAALRVLFYLPLLGPSSGFV
                     YGVPLVIAGFALIGHALRERRPSLALFAGVFFNATITIAFLLAVASVQGSMDRIVLVR
                     LVQLNVITLAVYLLPWLSARRRWQSALDQRASRVADYLVKLQLWLAISLSVVLLLPAF
                     CMIVWFPDVAEGGTAAVGSYLGWLSFASIVVAAIWFRPFSPWALACSLFAVSCLIAFN
                     FAAVSGWAGLHILTLCIAGTAWLMFVAAELTRNRWQATARDVSAIAGSLAVCLTLRGL
                     MHEPYATAWWSIGPLFSITALAVALNLRTKARGYVFAAGILFSLSTLLWWLLVGQERF
                     ELREFLLVNLIANSLAGVIWLLLDLRSRKHESSPSYYFSFHNIAALLSLCILALVAFA
                     MPYLDLNPQLIWLAFVCVVTLIMACLWDVSAKFAVAGVYILGLMGVAIALRQLDMSPR
                     RFAWCATIFLAAYSVCTALLWHWRSRITAFTRRIGVPERISADTSELPWFARFTVFAV
                     VALGMSAYLMSLHFLDFDLRGSAAIAFAAQSLTLGLLAEGWSARRWRRGAIAVFVLGL
                     VFFGWSWLTPFLDATWLNRSVSLMLVVFGLTSVYAILLKRATRHFPDWTNAARACVPW
                     LLGAGVVALFLCLGTEVRYQLDFGVVLINPLTLVAIGMVLLASVVMGVFCAVSPGHDP
                     LALSERGRMRYVYVAEVMLALLFLHVRLTMPWLFTGFIERFWPIAIMSIAFFGVVISE
                     ALRRRQIIVLAQPLERTGAFLPLLPVLGFWIATSEVDFSLLLFIVGGLYGLLSILRRS
                     FGFGLLAAVAGNGGLWYMFQRTADYGFFQHPQLWLVPAALSVLAAAYLNKDKLGEDQL
                     LAVRYLSLVTIYSSSTADVFINGVANSPWLPLILGSFSLAGIFAGIVFRVRGMTLLGA
                     VFLLLSIVTMIWYASENFGWTWLWYVFGIVTGATIILTFGVFEKKRHTADLRG"
     gene            complement(3400..6402)
                     /locus_tag="VFH96_05445"
     CDS             complement(3400..6402)
                     /locus_tag="VFH96_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET6973447.1"
                     /translation="MTLSRPFRSLFILALSLSTFAIIANAQIKPGRDPNQPIDQEYTN
                     KIREYTTAPYFNSPLTDYLPASSDVPTPKAVLGDVAGAPGKLPYAEDVYKYMRMLERA
                     SPNRVKVFSIGTTEEGREMIAVAIGSDQLISKIDENRARLTKLADPRTINMDEAEADR
                     LVNASFPIYYITGTIHSPETGSPTALMELAYRLVVDNSPYVKSIRDSVLTLITPVVEV
                     DGRNRMVDVYNWHLAHPNQNWPGLVYWGHYVAHDNNRDAIGLSLKLTRNVMNTYLAWK
                     PQVLHDLHESVPYLYDNTIGDGPYNAWVDPILTDEWQMIGWNNVSEMTKFGMPGVFAH
                     GNFDTWSPGYLMFIAATHNGISRLYETFGNGGADTVERTLSPSEYQRTWYRQNPPLPK
                     AKWSQRNNNNYQQTALLTSLYYFGNNSKLFLKNFYLKSKRSIDKPKQEGPAAYVLPGD
                     DPRPGNQAELLRTLQAQGVELSRATAPFTVTLRKKAAAPARSSSSPSASPSPAPKTEE
                     RTFPAGSYIVRMDQPYSRIADALLDYQYWAPNDPQTDIYDDTAWTMGELGNVEVVRVV
                     DTKVLTAPMDRVTGEVRSPGGITSAGAVYVINHNADNALVTLRYRLKDVEMEAAEEPF
                     EAAGRKFNRGSFIVRKANNEDLRRVANELGIQVYAVADAPSVKTHPLRAPRIAMMHTW
                     LSTQDEGWYRIGLDQLQVPFAYISTQDVSREPNLKSKYDVILFAPVGRGAQAIINGLP
                     MYSNPLPWKKTQLTPNLGSIDETDDMRPGLGWNGLQNLQKFVKDGGLFITVDDTSDFA
                     VNYGFTAGVSVNRSQRLRAVGAILRTKAVDAASPIAYGYGDSLAMYCMNGPIFNINNG
                     VGGRGGRPAPRATGRGTPDDPDVPQGRPPADLPEPPARVEPWEAPPVVDEQLRNAIGL
                     IPPNQRPRVVLRYADSRDLFVSGLLDGGDEIAQRAAIIDVPSGNGHVLLFSTNPFWRG
                     QTKGSYFLVFNAILNWDNLNAGRKLAEK"
     gene            6495..8735
                     /locus_tag="VFH96_05450"
     CDS             6495..8735
                     /locus_tag="VFH96_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HET6973448.1"
                     /translation="MDTKIPKAAGGAPAIITAVKTISEEMGLVRGVRTLLKVNQTGGV
                     DCPGCAWPEPDRERSHFEFCENGAKHVADEATTKRVTPEFFQQWSVAELLEQSDQWLN
                     AQGRLTHPMLLHRDATHYEPISWDDAFELLAGELNSLNYPDQAIFYTSGRTSNEAAFL
                     YQLFVRQFGTNNLPDCSNMCHESSGSALSETVGVGKGTVTLEDFEEAQVIFVVGQNPG
                     TNHPRMLTSLERAKRRGCKLVHINPLPEVGMTRFKHPQDVLGLLGSGTILADLFLQVR
                     INGDVALLKGITKAVLAHQDALDSDFIAQHTTGFDEFVSALQAVSWSEIVEQSGVGRR
                     QIEEAAQIFLESERTIFCWAMGLTQHRNAVANIQEIVNLMLLRGQVGKPGAGLCPVRG
                     HSNVQGDRTVGIWERPTEAFLDKLGEVFDFEPPRNHGFDTVRAIHAMHEGQGKVFFAL
                     GGNFLSATPDTDFTAAALRRCRLTAHVSTKLNRSHLITGDRALILPCLGRTEIDVQAT
                     GLQVVTTENSMGVVQVSRGSLQPASEELLSEPRIVARLARATLEHRTTVNWEELASDY
                     DKIRAAIERVIPGFDDYNRRVRERGGFYLPNAARNRVFNTKSGKAQFTVHELPQHDLG
                     PDQFLMMTIRSHDQFNTSIYTENDRYRGISDGRRVVFLNGEDIAAAGLKAQQVVDLVS
                     HFEGEERIARAFIVVPYDIPRRCAATYFPEANVLVPVRHVALKSNTPVSKSVVISIRP
                     ATEEPE"
     gene            8736..>8980
                     /locus_tag="VFH96_05455"
     CDS             8736..>8980
                     /locus_tag="VFH96_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973449.1"
                     /translation="MSAQTVKKRAKKKAPTKKTSKKLKAATTSKNNVKPKLYASLVVQ
                     LMSLLKGEHDFIANAANFSALLFSSLPNVNWAGFYFL"
BASE COUNT         1648 a         2497 c         2671 g         2164 t
ORIGIN      
        1 gattcaacgg acgggtggcc acagagggcc acccctacag ttgacccaac gtgggcgcga
       61 ttacttgatg gtagatatgc ggtcgatcga tttctgttgt gggcgttaat gccggcgttt
      121 ctgttgctcg cggtttatgg agcgctttcg ggtgtgacgc aggagctggc cgggtatgcc
      181 ggtttcgaca ttgccggatt tccacacgcg gaagcgtttg ggctcggctc gtggatcgtc
      241 ttcgggctgt tgctcgtcgc gatgatcggg agcttttggg aacgacggag caatgcttat
      301 ttgctcggcg cgttggctgt actcgcagcg gcgattccgt tgttggccgg tcgattcgaa
      361 agtcagatcg caactgcgac ggcgtggcgc gggctggcag cgatgtttct gctgctcggg
      421 tcgattgtga tctggtcgcg gcgcaagatc ttcggcgcag gcttccttga ggaagtacga
      481 gccgtactgt tcactctgac gatcctgccg ctgctaatcc tcacgagctg tgctgcctta
      541 cgagtccttt tctacttgcc actgctggga ccgagcagcg gcttcgttta cggcgtcccg
      601 cttgtgatcg cgggcttcgc gttgattggt catgcactgc gcgagcgtag gccaagtctc
      661 gccttgtttg cgggcgtttt cttcaatgcg acgatcacca ttgcgttctt gctcgccgtc
      721 gcttcggtgc aaggctcgat ggatcgcatc gttctcgttc gtctcgtgca actgaatgtg
      781 atcactctcg ctgtctattt gctgccgtgg ttgagcgcgc gacggcgctg gcagtcagct
      841 ttggaccagc gggcctcgcg cgtcgctgac tacctggtca agctgcaact atggttggcg
      901 atcagtttga gtgttgttct attgctcccc gcgttctgca tgatcgtgtg gttccccgat
      961 gttgctgaag gtggcacggc ggcggtcgga agctatctcg gttggttgag tttcgcgtcg
     1021 atagtagttg ctgcgatttg gttcaggccg ttttcgccgt gggcgcttgc gtgttcgtta
     1081 tttgcagtca gttgtttgat tgcattcaac tttgctgcgg taagcggctg ggcgggtttg
     1141 cacatactga cactctgcat tgccggaaca gcatggttaa tgtttgtcgc cgccgagctg
     1201 acaaggaata gatggcaggc gaccgcgcgc gatgtatcgg cgattgctgg tagtctcgct
     1261 gtgtgtctca ctctgcgcgg attgatgcac gaaccgtatg cgactgcgtg gtggtccatc
     1321 ggtcctttat tctcgatcac agcgcttgca gtcgcattga acttgaggac aaaagcgcgc
     1381 ggctacgtct ttgccgccgg tattctgttc agcctctcaa cgttgctgtg gtggttgctc
     1441 gtcggtcagg aacgttttga gctccgtgag tttctgctcg tcaatctgat cgcgaactcg
     1501 cttgcaggtg tgatctggct cctgctcgat ctgcgctcac gaaagcatga atcatcaccg
     1561 agctactatt tttcatttca caacattgcc gcacttcttt cactctgcat actcgcgctc
     1621 gttgcgtttg ccatgccgta tctggatctc aacccgcagc tgatctggct ggcttttgtt
     1681 tgtgtagtta ccttgataat ggcgtgtttg tgggacgttt cagcaaaatt tgcagttgcc
     1741 ggtgtttaca ttcttggttt gatgggcgtt gcaattgcgc tccggcagtt agacatgagc
     1801 ccaaggcgtt tcgcctggtg cgccacgatt ttcctggcgg cctacagtgt ctgcactgcg
     1861 cttttatggc actggcgaag ccgaatcacc gctttcacac gccgaatcgg tgtgcctgag
     1921 cgtatcagtg ccgatacgtc cgagcttccg tggtttgcga gattcaccgt atttgccgtg
     1981 gtcgcacttg gtatgtccgc ctacttgatg tcgcttcact tcctcgactt cgacttgcgc
     2041 ggttctgccg ctatcgcctt cgccgcacag tctcttaccc ttggactgtt agcagaggga
     2101 tggtccgcgc gacgttggcg gcgcggcgcc atcgcggttt ttgttctcgg tcttgtcttc
     2161 tttggctggt cgtggctaac gccgttcctc gacgcgacgt ggctaaatcg cagcgtgagc
     2221 ttaatgctcg tagtgtttgg tctgacgagc gtttacgcaa ttcttctcaa acgggcgacc
     2281 aggcattttc ctgactggac taacgcggcg cgcgcttgtg tgccgtggtt gctcggcgcc
     2341 ggtgtcgttg ccctgttctt gtgtctcgga actgaagttc gttatcagct cgacttcggc
     2401 gtcgtgctca tcaatccgct tacgctcgta gcgattggca tggtgctgct cgcgtcagtg
     2461 gtcatgggcg tgttctgcgc cgtgtcgcca ggccacgatc cgttagctct ttctgagcgt
     2521 ggtcgtatgc gatacgtgta tgtcgccgag gtgatgctgg cgctgctgtt tctgcacgtg
     2581 cgtttgacga tgccatggct gttcacaggc tttatcgaac gcttctggcc cattgccatc
     2641 atgtcaattg cattcttcgg tgtggtgatt agtgaagcac tgcgacgacg ccagatcatc
     2701 gtgttggccc agccgctgga acgcactggc gcgtttctgc cgctcctgcc agtgctgggg
     2761 ttctggatcg cgacgtcaga agtcgacttc tcgttactgc tgtttatcgt cggcgggctt
     2821 tacggattgc tatcgatcct gcgtcgctcg tttggttttg gtttgttggc ggcggtggct
     2881 ggaaatggtg gactctggta catgttccag cgcactgctg attatggatt tttccaacac
     2941 ccgcagttgt ggctggtccc ggccgcgctc tccgttctcg cagcggccta cctgaacaaa
     3001 gacaagctcg gtgaggatca gttgctggcc gtgcgatacc tatcgctggt cacgatctac
     3061 agctcatcaa ccgccgacgt cttcatcaac ggcgtcgcga attccccctg gctgccattg
     3121 atactcggat cgttctcgct cgccggcatc ttcgcgggca tcgtatttcg cgtccgcggc
     3181 atgacgctgc tcggcgcggt ctttctgttg ttatcgatcg tgacgatgat ctggtacgcg
     3241 tcggaaaact ttggctggac gtggctgtgg tacgtcttcg ggatcgtgac gggcgccaca
     3301 atcattctga cgttcggtgt ctttgagaag aaaagacata ccgcggatct gcgcggataa
     3361 acgcagatct gcgttcatcc gcgtaaatcc gcggctaact tatttctccg ccagcttgcg
     3421 gccggcgttg aggttgtccc agttcagaat cgcgttgaag acgaggaagt agctgccctt
     3481 cgtctgcccg cgccagaatg gattggtcga gaacagtaac acgtgtccat tgcccgaagg
     3541 cacatcgatg atcgccgccc gttgcgcgat ctcatcgccg ccgtccaaca agccggaaac
     3601 aaacagatcg cggctgtcag cgtagcgcag gaccacgcgc ggacgctggt tcggtggtat
     3661 caacccaatc gcgttgcgta actgctcgtc tacgaccggt ggcgcttccc acggctcgac
     3721 tcgtgccggt ggctccggaa gatctgccgg tggtcttcct tgtggcacgt ccggatcatc
     3781 cggagtcccg cgaccggtcg cgcgtggcgc tggtcgtccg ccgcgcccac caacaccgtt
     3841 gttgatgttg aagattggac cattcatgca atacatcgcg agcgagtcgc cgtagccgta
     3901 agcgatcgga ctcgccgcgt cgacagcctt cgttcgcagt atcgctccga ctgcgcgtag
     3961 acgttgcgaa cgattcacgg aaacccccgc cgtaaacccg tagttcaccg cgaagtcgga
     4021 cgtgtcgtcg acggtgatga acagcccacc gtctttcaca aacttttgca agttttgcaa
     4081 accattccaa ccgagccccg gtcgcatgtc gtcggtttca tcgatgctgc cgaggttggg
     4141 cgtaagctgc gtcttcttcc acggcagcgg attgctgtac atcggcaggc cattgatgat
     4201 cgcctgtgcg ccgcgtccga cgggcgcaaa cagaatcacg tcgtacttgc tcttgagatt
     4261 cggctcgcgc gaaacatcct gcgtgctgat gtatgcgaaa ggcacttgta actgatcgag
     4321 accgatgcgg taccagcctt cgtcctgcgt gcttagccac gtgtgcatca tcgcgatgcg
     4381 cggcgcgcgc agcgggtgcg tctttactga cggcgcatcc gcaaccgcat acacctgaat
     4441 gccaagctcg ttcgcaactc gtcgcagatc ctcgttgtta gctttgcgaa cgatgaacga
     4501 gccacgattg aacttgcgtc cagcagcttc gaaaggctct tctgcagctt ccatctcgac
     4561 gtctttcaaa cgataacgca gcgtcacgag cgcgttgtcg gcgttgtgat tgataacgta
     4621 gaccgctccg gcactcgtga tcccgcccgg cgagcgcacc tcacccgtca cgcggtccat
     4681 cggcgccgtc agcactttcg tatcgaccac acgcacgacc tcgacgtttc ccagctcgcc
     4741 catggtccag gcggtatcgt cgtaaatgtc tgtttgcgga tcgtttggcg cccagtattg
     4801 atagtcgagc aacgcatcgg cgatacggct gtacggctgg tccatgcgga cgatgtagct
     4861 gcccgccgga aacgttcgct cttcggtctt cggcgcggga ctcggcgaag ctgaaggcga
     4921 cgatgaagag cgggccggag cagcggcttt ctttctcaac gtaactgtga acggcgcagt
     4981 cgctctgctg agctcgacgc cctgtgcctg cagcgtgcgc agcagctccg cctgattgcc
     5041 cggtcgcgga tcgtcgccgg gaagaacata agccgcgggg ccctcctgct tcggcttgtc
     5101 gatcgagcgc ttgctcttta aatagaaatt tttgagaaac agcttgctgt tgttgccgaa
     5161 atagtaaagc gacgtcagca atgcggtctg ctggtaatta ttattgtttc gctgtgacca
     5221 ctttgctttc ggcaacggcg ggttctgtct ataccacgtg cgttgatatt cactcggcga
     5281 taacgttcgc tccaccgtat cagcaccccc gttgccgaag gtttcgtaga gacggctgat
     5341 gccgttgtgc gtcgccgcga tgaacatcag gtaaccgggc gaccacgtgt cgaagttgcc
     5401 gtgtgcaaac acgccgggca tgccaaactt cgtcatctcc gaaacgttgt tccagccgat
     5461 catctgccac tcgtcggtca gaatcggatc gacccacgcg ttgtatggac catcgccgat
     5521 cgtgttgtcg taaagatacg gaactgactc gtgcagatcg tgaagcacct gcggtttcca
     5581 cgcgagatac gtgttcatca cgttgcgcgt gagtttcagc gagagtccga tcgcgtcgcg
     5641 attgttgtcg tgggcgacgt agtggcccca gtagaccagt cccggccagt tctgattcgg
     5701 atgcgcgagg tgccagttgt aaacatcgac catgcgattg cggccatcga cttcgaccac
     5761 cggcgtgatc aacgtcaaga cactgtcgcg gatcgacttc acatacgggc tgttatcgac
     5821 gacgagccgg taggccagct ccatcaacgc ggtgggcgaa ccggtttcgg gcgagtggat
     5881 cgtacccgtg atgtaataga ttggaaacga agcgttgacg aggcggtccg cttcggcttc
     5941 gtccatgttg atggttcgcg gatcggcgag tttcgtgagt cgcgcgcgat tctcgtcgat
     6001 tttggaaatc agttggtcgg agccgatcgc caccgcgatc atctcgcgtc cttcttcggt
     6061 ggtgccgatc gaaaagacct tcacgcggtt gggactggcg cgctcgagca tgcgcatgta
     6121 cttgtaaacg tcttccgcgt acggcaactt gcccggcgcg ccggccacgt cgccgagcac
     6181 ggctttcggc gtcggtacgt cagacgatgc gggcaggtag tcggtcagag gagagttgaa
     6241 gtacggcgcg gttgtatact cgcgaatctt gttcgtgtac tcctgatcga tcggttgatt
     6301 gggatcgcgg cccggcttga tctgggcgtt ggcgataatt gcgaaagttg aaagggagag
     6361 agctaagata aagagcgacc taaaggggcg cgacagggtc atataggttt ttcctctaga
     6421 gtttgcttgt caggcttcta gcacagatta cacggatttt gaaattcggg gaatctgtgt
     6481 aatctgtgta atcggtggat acaaaaatac caaaggctgc cggtggagcg ccggcgatca
     6541 tcacggcggt caagactatc tcggaagaga tggggctcgt gcgtggtgtg cgcacgctgc
     6601 tgaaagtgaa tcagacgggc ggcgttgatt gtcccggttg cgcgtggccg gagcccgatc
     6661 gcgagcgctc gcattttgag ttttgcgaaa acggcgcgaa gcacgtcgcc gatgaagcga
     6721 ccaccaaacg cgtcacaccc gagttctttc agcagtggag cgtcgccgag ctcctggagc
     6781 aatcagatca atggctcaac gcacagggac gactcacgca cccgatgctg ctgcatcgtg
     6841 atgcgactca ttacgaaccg attagctggg acgatgcttt cgagctgctc gcgggcgagt
     6901 tgaactcact caactatccc gaccaggcga tcttttacac ctccgggcgt accagcaacg
     6961 aagcggcgtt tctttatcaa ctgttcgtcc gccagtttgg cacgaacaac ctgcccgact
     7021 gctcaaacat gtgtcacgag tcgagtggct ctgcgttgag cgaaacggtc ggcgtcggga
     7081 agggaacagt cacgctcgaa gatttcgaag aggcgcaggt catcttcgtc gtcggccaga
     7141 atccgggcac gaaccatccg cgtatgctga cgtcgctcga gcgcgctaag cgacgcggtt
     7201 gcaaactggt ccacatcaat cccctcccgg aagtcgggat gacgcgcttc aaacatccgc
     7261 aggatgtgct cgggctgctc ggctcaggca caatcctcgc ggatctgttt ctccaggtgc
     7321 ggatcaacgg cgacgtggcg ctgttgaagg gaatcacgaa agccgtgctc gcgcatcaag
     7381 atgcgctcga ttccgacttc attgcgcagc acacaacggg tttcgatgag tttgtctcgg
     7441 cgctgcaagc agtttcgtgg agcgagatcg tcgagcagag cggagtcgga agaaggcaga
     7501 tcgaggaggc cgcacagatc ttcctcgagt cggagcgcac gattttctgt tgggccatgg
     7561 gactgaccca acatcgcaat gccgtcgcga acattcagga gatcgtcaac ttgatgctgc
     7621 tgcgcggaca ggtcggaaag cccggcgctg gtttgtgtcc ggtgcgtggt cacagcaacg
     7681 tgcagggcga tcgcaccgtc ggcatctggg aacgaccgac tgaagcgttt ctcgataaac
     7741 tcggcgaggt gtttgacttc gaaccgccgc gaaatcacgg cttcgacaca gtgcgtgcga
     7801 ttcacgcgat gcacgagggt cagggcaagg tgttcttcgc gctcggcggc aactttctgt
     7861 cagcaacgcc tgatacagat ttcactgccg cagccttgcg tcgttgtcgc ctgacggcgc
     7921 acgtctcaac gaagctcaat cgatcgcatt tgatcacggg ggatcgcgcg ctgatcctgc
     7981 cgtgtctggg tcgcacagag atcgacgtgc aggcaaccgg tctacaggtt gtcacgacgg
     8041 agaattcgat gggcgtggtg caagtctcgc gtggctcgct ccagcctgcc tcggaggagc
     8101 ttctgagtga gccgcggatc gtagcccgac tcgcacgcgc tacgctcgaa catcgcacca
     8161 cggtgaactg ggaagagctc gccagcgact acgataaaat tcgcgctgcg atcgagcgcg
     8221 tcatcccggg tttcgacgat tacaatcgac gcgtgcgcga gcgcggcggc ttctatcttc
     8281 cgaacgccgc gcgcaatcga gtcttcaata cgaagtcggg taaggcgcag tttacggtgc
     8341 acgagttgcc gcaacacgat ctcggcccag atcagttttt gatgatgacg attcggagtc
     8401 atgaccagtt taatacgtcg atctacactg aaaacgatcg ttatcgcggc atcagcgatg
     8461 gccgccgtgt cgtgtttctc aacggagagg acatcgccgc ggcgggcctg aaagcgcaac
     8521 aagttgtaga cttggtcagc catttcgaag gtgaggagcg catcgcccga gcgtttatcg
     8581 ttgtccctta cgacataccg cgtcgctgcg ctgcgactta ctttcccgaa gcgaatgttt
     8641 tggtgccggt gcgtcatgtc gcgctgaaga gcaacacgcc agtttcaaaa tcagtagtga
     8701 tcagcatccg gcctgcaacg gaggagccag agtaaatgtc cgcccaaaca gttaagaaaa
     8761 gagcgaagaa aaaagccccc acaaagaaaa cctcaaagaa gctgaaagcc gctaccacgt
     8821 cgaagaacaa cgtgaagccg aagctctacg cgagtctcgt cgtgcagttg atgagcttgc
     8881 tgaaaggcga gcacgacttc atcgcgaatg ccgcaaattt cagcgcgttg ttattcagct
     8941 cactaccgaa cgtgaactgg gccgggttct atttcctgca
//
LOCUS       DASSFQ010000160         5399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_971329, whole genome shotgun sequence.
ACCESSION   DASSFQ010000160 DASSFQ010000000
VERSION     DASSFQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971329"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFH96_05460"
     CDS             <1..618
                     /locus_tag="VFH96_05460"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6973450.1"
                     /translation="AKPRRRVWPWVVGIAGAFVLGLLAISVAALIMVPRLKTRQNERT
                     VAPIHDPSNSNTVPGNINANSNSTGNVNTPPPANHDQVLAHLKNLENEWMVANVNADK
                     KKLDLILADDYVGQSGPEGGLETKAEYLKRTERDDQIQKWEMSDEKLTLTGDRATLAG
                     IVTFFLRDGRVGSFDFKDKFVWRDSRWQATGSELTPRKPPVGTDL"
     gene            649..1170
                     /locus_tag="VFH96_05465"
     CDS             649..1170
                     /locus_tag="VFH96_05465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET6973451.1"
                     /translation="MSIITDVIESTKAIIQGMGVTLSYIPKRKWTVEYPDDPVTLQPR
                     YRGQHLLHVDELGREKCVACYLCAAACPSECIYIVAADDPRPPDQRIGVDERYAEVYN
                     IDYGRCIFCGFCVEACPKDAITHGYNFELSVYSRADLLKTKDDLLITKQLQSKNYRVA
                     QSHEDVDSEYKEV"
     gene            1225..3051
                     /locus_tag="VFH96_05470"
     CDS             1225..3051
                     /locus_tag="VFH96_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6973452.1"
                     /translation="MALREYHKRWEFDLKSSPERLWPYIADTNRFNRDTGVPQIDVAP
                     ADKQLRNARRKIRLGFYGLPVEWEEQPFEWVKPVRFGVERVYSKGPLARLRMRAELKP
                     NSGGGTHLIYEVWSTPRNIPGAVAIPMQLNFVVARRFREYIQKYDDVAFQGVALETAA
                     PNTSQSSFDLQRLATLKQKLIADLDTEQKLAVGERLLQFLEHGDDFEVSRIRPYKLAD
                     EWHEPRRLVLEVCLRATRVGLLDFQWDLLCPLCRGPQESGLSLKDIKTDVHCETCKID
                     FTVNFDRYVELTFRPNPSVRSVSVSPYCIGSPNWTPHVVAQQLLPAGNTRELLLPLES
                     GSYRLRALELPGSQDVTVSQEGEASAHVTLSRNGWSEEPLNVNEEFTLTLQNATDAEQ
                     LVILERLEWSDQATTAAEVTALQMFRDLFAAEALRPGEQISVGTLTVLFTDLRNSTQL
                     YREIGDATAFGRVMNHFDVVRKSIADHDGALVKTIGDAVMAVFRSAADGLLAMLEVQQ
                     ALSEPGDGGRPLQLKAGLNTGPCIAVTLNDRLDYFGSTVNMAARLECLSSGSDVIISR
                     SVYEDPRVQELIESDSFDAEPFDMKLKGFENESFELWRVSRH"
     gene            3051..3725
                     /locus_tag="VFH96_05475"
     CDS             3051..3725
                     /locus_tag="VFH96_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973453.1"
                     /translation="MPALLAAQEVSKIYQMGSNNVTALDNVSLEINEGEFVAIQGTSG
                     SGKSTLLNMLGGLDHPTKGEVYFASRPLGPFTKKEMARYRRFSVGMIFQNFNLIPTMT
                     ARENVSLALAFGGLRGQQRRARSRELLARVGLAERVEHRPSELSGGEQQRVAIARALA
                     NNPKVLLADEPTGNLDSIRARELLSLLRDMVTQDSLTILMVTHDHELANSFADRIVLM
                     KDGRVV"
     gene            3777..4625
                     /locus_tag="VFH96_05480"
     CDS             3777..4625
                     /locus_tag="VFH96_05480"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET6973454.1"
                     /translation="MQPASQMTLEALRSVPLFASLDDDAARELRSLLSDKRVPQNTRL
                     FRQGDKGDAMYLIESGRVRISIRDEEEQEVVLAELAQGDFFGEMSIIDGRQRSADAKV
                     IDDAQLAILSREAFLSFVRSNADVALEMLSALTDRLRRTDELLRSRVSRNANEEEKAR
                     LTVADRAADLIAEFGGSWKFIILSIALIIVWIIFNTFILIRGFDPAPYAMLNLVLAVI
                     AGMQAPIIMMSQNRQGEKDRLRADLDYQVNLKNELSLAEVLRRLDVLESERLPKLIDD
                     MRAQIK"
     gene            4622..5311
                     /locus_tag="VFH96_05485"
     CDS             4622..5311
                     /locus_tag="VFH96_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET6973455.1"
                     /translation="MIRAIFFDFNGVIIDDETIQMKAYQEVLSGHEIALTEELYFGAL
                     GMDDKTFVRSMFAHAKKPLSDPLLEEVLGAKTDLHRQMIEELPLFPGVLKFLKAASRE
                     FQLGLVSMANLTEVSYVFQRANLTPLFSVIVTAEDASVCKPAPDCYLTGLLKLNELRQ
                     RERELPLLASECLAIEDSPPGIQSARAAGMRTLGVTNTVSEEALRAAGAEVVTKSLAD
                     WNVDAVKLVFT"
BASE COUNT         1210 a         1397 c         1595 g         1197 t
ORIGIN      
        1 gcaaaaccgc gccggcgcgt gtggccatgg gttgtcggca ttgcgggcgc gttcgtcctt
       61 ggcctgctgg cgatctcggt cgcggcgctg attatggttc cgcggttaaa gacgaggcag
      121 aacgagcgaa cggtggcgcc aatccacgac ccgtcgaact cgaacactgt tcctggcaac
      181 atcaacgcaa acagtaattc gactggaaac gtcaacactc caccacccgc gaatcacgac
      241 caggtgttgg cccacttgaa gaatctcgag aacgagtgga tggtggcgaa cgtcaacgcc
      301 gacaagaaga aactcgatct aattctggcg gatgattatg tcggccagtc aggacctgaa
      361 ggcggactgg aaacgaaagc tgagtatctg aagagaacag agcgtgacga tcaaatccag
      421 aagtgggaga tgagtgatga gaaactgacg ctgacgggag atcgagcgac gttagcggga
      481 atcgtcacgt tctttcttcg tgacggtcgg gtgggaagtt ttgattttaa ggataagttt
      541 gtctggcgcg atagcaggtg gcaggcgacc ggctctgaac tgacgccacg aaaaccgcct
      601 gtaggcactg atctatagcc acaaaaaggc acaaaagcac aaagacacat gtcgattatc
      661 actgacgtta tcgagtcaac taaagcaatc atccagggca tgggtgtgac gctgtcttac
      721 atacccaagc gcaagtggac ggtcgagtat cccgacgatc cggtgaccct gcagccgcgt
      781 tatcgcggtc agcacctttt gcacgtcgac gagctcgggc gcgagaagtg tgtcgcctgt
      841 tatctctgcg cggcggcgtg cccttccgag tgtatttaca tcgttgccgc cgacgatccg
      901 cgaccgcccg atcagcgcat cggtgtcgac gagcgttacg ctgaggtcta caacatcgac
      961 tacgggcgtt gcatcttctg cggcttctgc gtcgaagcgt gcccgaaaga cgccatcact
     1021 catggctaca actttgagct ctctgtttac agccgcgccg atcttctcaa gaccaaagac
     1081 gatttgctga tcacaaagca gctacagtcg aagaactacc gggtggccca atcgcatgaa
     1141 gatgtcgatt cggagtacaa ggaagtttga ccatccacag attacgcaga tacgaatccg
     1201 tgtaatctgt ggataaacct tgacatggcc ttgcgcgagt atcacaaacg atgggagttt
     1261 gatctcaaat ccagtcctga gagactttgg ccttacatcg ccgacaccaa ccggttcaat
     1321 cgcgacacag gcgtgccgca aatcgacgta gctcccgccg ataaacagct tcgcaacgcg
     1381 cgccgcaaaa tacgactcgg attctatggt ctgcccgtcg agtgggaaga gcagccattc
     1441 gaatgggtga agccggtgcg ttttggtgtc gagcgtgttt acagcaaagg tccactcgcg
     1501 cgtttgcgaa tgcgcgcgga gttgaagcca aactccggcg gcggcacgca cttgatttac
     1561 gaagtgtggt cgacgccgcg aaacattccg ggcgcggtcg ctataccaat gcaactgaat
     1621 ttcgtcgtgg cgcggcggtt tcgtgagtac attcagaaat atgacgacgt tgcgtttcag
     1681 ggtgtagcac tcgagactgc tgcgccaaac acttcacagt cgtcgtttga tctgcaacgc
     1741 ctcgccacgc tcaagcaaaa actgatcgcg gatctcgaca ctgaacagaa gcttgcggtg
     1801 ggcgagcgtt tgcttcaatt tctggagcac ggtgacgatt tcgaagtctc gcggattcga
     1861 ccgtacaagc ttgcggacga atggcacgag ccgaggcgtt tagtgcttga agtttgcctg
     1921 cgcgcgacgc gggttggcct cctcgatttt caatgggatc tattgtgtcc cctctgtcgt
     1981 ggtccacagg agagcgggct gtcgttaaag gatattaaga cagatgtcca ttgtgaaacg
     2041 tgcaagatcg atttcactgt caacttcgat cggtatgtgg agttgacgtt tcgtccgaat
     2101 ccttcggtgc ggagcgtgtc agtgtcgccg tactgcatcg gcagcccgaa ctggacgccg
     2161 cacgttgtcg cgcagcaact gctgcctgca ggaaacacac gtgagctgtt gttgccgctc
     2221 gaatccggca gctatcgact gcgtgcgctc gagctgccgg gatcgcagga cgtcacggtg
     2281 tcacaggaag gcgaagcgtc ggcgcacgtt acgctttctc gtaatggttg gagtgaagag
     2341 ccgttgaatg tgaacgagga gtttacgctc accttgcaga atgcgactga cgccgagcag
     2401 ctcgtgattc tcgaacgact cgaatggagc gatcaggcga caaccgcggc tgaagtaacg
     2461 gcgctgcaaa tgttccgcga tctattcgcc gctgaagcac tgcgacccgg cgagcagatc
     2521 tccgtcggca cactgactgt actcttcact gacctgcgca actcgacaca actctatcgc
     2581 gagatcggcg acgccactgc gttcggacgc gtgatgaatc atttcgacgt cgtgagaaaa
     2641 tcgattgcgg atcatgacgg cgctctcgta aaaacgatcg gcgatgcagt gatggcagtg
     2701 tttcgatcgg ctgccgacgg tttgctcgcg atgcttgaag tacagcaagc gctctctgag
     2761 ccgggcgacg gcggcagacc gctgcaactc aaagccgggc tcaacaccgg accgtgcatc
     2821 gccgtgacgc tcaacgaccg gctcgactat tttggctcga ctgtaaacat ggctgcgcgt
     2881 ctcgaatgtc tctcgtcggg ttctgacgtg ataatctcac gctcggttta cgaagatccg
     2941 cgagtgcagg agctgatcga gtcagacagt ttcgacgcag agccgtttga tatgaaattg
     3001 aaaggtttcg agaacgaaag ttttgagcta tggagggtca gtaggcactg atgcccgctc
     3061 tcctggccgc tcaggaagta tcaaagatct accagatggg cagtaacaat gttaccgccc
     3121 tcgacaacgt ctcgctcgag atcaatgaag gcgagttcgt cgctatccag ggaacgtccg
     3181 gttccggcaa gtcgacttta ctaaacatgc ttggaggact cgatcacccg accaagggtg
     3241 aagtgtattt tgcgtcgcga ccgctcgggc cgttcacgaa gaaagagatg gcgcgttacc
     3301 ggcgcttctc ggtcgggatg atctttcaga acttcaatct cattcccacg atgacggcgc
     3361 gcgagaacgt ttcgctagcg cttgcgtttg gcggtttgcg tggacagcag cgacgtgcgc
     3421 gctcacgtga gctgctggcg cgtgttggtc tggcagagcg agttgagcac aggccttcgg
     3481 agctttccgg cggtgagcaa cagcgcgtcg cgattgcacg tgcgctcgcg aacaatccga
     3541 aagtgttgct ggcagatgag ccgaccggaa atctcgactc gatccgcgcc cgcgagctct
     3601 tatcgttgtt gcgcgacatg gtgactcagg attcgttgac gatcctgatg gtcacgcacg
     3661 atcacgagct ggcgaacagc ttcgcggatc gcatcgtgtt gatgaaggat ggaagagttg
     3721 tataggtcct ataggactaa taggacctgt aactaattta acccttcgga ggatttatgc
     3781 agcctgcttc tcaaatgacg ctcgaggcgc tgcgatcggt gccgctgttc gcctctcttg
     3841 acgacgatgc ggcgcgcgag ctgcgcagtt tgctttccga taagagagtg ccgcagaaca
     3901 cgcgtctctt tcgtcagggc gacaagggcg acgcgatgta tctgattgag agcgggcgcg
     3961 tgcgtatcag cattcgcgac gaagaagagc aggaagtggt tctggccgag ctggcgcagg
     4021 gcgacttctt cggcgagatg tcgatcatcg acgggcgaca aagatcggcg gatgcgaaag
     4081 tcatcgacga cgcccaactc gcaatcctct cgcgcgaagc gtttctctcg ttcgtgcgca
     4141 gtaatgcaga tgttgccctc gagatgttga gcgcgctcac tgacaggctg cgacgaactg
     4201 acgagctctt gcggtcaaga gtttcacgca acgcaaacga agaagaaaaa gcgcgtttga
     4261 ctgttgccga tcgcgcggca gatttgatcg cggagtttgg tggtagctgg aaatttatca
     4321 tcttgtcgat cgcgctcatc atcgtctgga tcatcttcaa tacattcatt ctgattcgcg
     4381 gcttcgatcc ggcgccgtat gcaatgctga atctcgtgct cgcggtgatc gccggcatgc
     4441 aggcgccgat catcatgatg tcgcagaatc gacagggcga gaaagatcga ctccgcgcgg
     4501 acctcgacta ccaggtaaat ttgaagaacg agctctcgct ggccgaagtg ttacgtcgtt
     4561 tggatgtgct cgagagtgaa cgcttgccga aactgattga cgatatgaga gcacagatca
     4621 aatgatcaga gccatcttct tcgacttcaa cggcgtcatc atcgacgacg aaaccattca
     4681 gatgaaggcc tatcaggaag tgttgagtgg ccacgagatc gcactcactg aggagttgta
     4741 tttcggcgca ctcggcatgg acgacaagac gtttgtccgc tcgatgtttg cgcacgcgaa
     4801 gaagccgttg agcgatccgt tgttggagga agtgctcggc gcgaagacag atctacaccg
     4861 gcagatgatc gaagagttgc cgctgtttcc cggtgtgttg aagtttctga aggcggcttc
     4921 acgcgagttt caactcggac tcgtgagcat ggcgaatttg actgaagtta gttacgtgtt
     4981 tcagcgcgcg aatctgacgc cgctcttttc ggtgatcgtg acggcggaag atgcgtcggt
     5041 gtgtaagcct gcgcctgatt gttatctgac gggattgttg aagctgaacg agttgcgaca
     5101 acgcgagcgc gagttgccgc tgctcgcatc tgaatgtttg gcgattgaag actcgccgcc
     5161 cggaattcaa tcggcgcgcg cggcgggtat gcggacgctc ggtgtgacga ataccgtttc
     5221 ggaagaagca ttacgcgcgg ccggcgctga ggtggtgacg aagagtctgg ctgattggaa
     5281 cgtcgatgcg gtgaagctgg tttttacatg attacaggat ttacagaatt aacaggataa
     5341 atcctctcat cttgcaaatc ctgtaatcct gtcaaactgt ctctgactat gagctttct
//
LOCUS       DASSFQ010000161        22032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1702712, whole genome shotgun sequence.
ACCESSION   DASSFQ010000161 DASSFQ010000000
VERSION     DASSFQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22032
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702712"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1149)
                     /locus_tag="VFH96_05490"
     CDS             complement(166..1149)
                     /locus_tag="VFH96_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973456.1"
                     /translation="MNADQSTELKGWLPVDAVVVDGRPGLMWMEMSGIDLTEPFLQQT
                     VERARRENRAARFTEFDVLLQLEKQLESVEPTGFIFHSSRCGSTLVANACRAVSNSIV
                     LSEANAIDKLVARFITDADNPVKESLYSVFLRSVVHALGQRRTDNERYLFIKFACCSF
                     AQLERIKRIWPNVPWLFLYRDPVETIVSNMSDIPPWLIDNDRRVLSSIIGDASEMSLE
                     ELCARTIGSLYSAAYQLANANSMLLNYNQLSVPVIASVLNFFNVSLASEELETIARTS
                     EVYSKEVSGTRAFVADVDAKQKLASDTAREMSERWAAEPYRLLEQKRNSNP"
     gene            complement(1167..2180)
                     /locus_tag="VFH96_05495"
     CDS             complement(1167..2180)
                     /locus_tag="VFH96_05495"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6973457.1"
                     /translation="MATFSEISFDDYGPRRLSVELANICNLHCSYCFRSDENLYSSHA
                     EFFPLDLLRRVIDEARDAAKVTRISFTGGEPTLHPEFAETLRIVGNAGATVSFVTNGW
                     HFERVWPALQENRAAVSHVAFSLDGVTREDHDRWRGNGSFDRLVRAFTRCYMSKLPFG
                     IKIVIRRDLVDQLEQIAIFAARMGAASLNFVHVMPTSNAVADNSALSLDEQRVAEQEI
                     AILARIFKMNIGIDVGYYNIDDTRPPCAPLAGVNMNIDYRGRLSLCCNLSGFRGAAQE
                     LDVVADLNVESFASAYEKFKTLATAQLQRRKDALAALHTPDVYTGSPCMFCLQSFGKI
                     PWH"
     gene            complement(2268..3233)
                     /locus_tag="VFH96_05500"
     CDS             complement(2268..3233)
                     /locus_tag="VFH96_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973458.1"
                     /translation="MESIQVFDRPVSQAFYSVAGRLLFIESSSLELRNLIVDLFAGWQ
                     LTPVSLPDRSPDIQISFSCEDLSQRIPRDLDQFEIADGGKCYTDGTGLYLEFGGPVVH
                     LENGDLVSVSFTELPRLGDPLFGRAASFAVCAALRRFGLFDLHSAGVVEPQSGKAVLI
                     IGPSGSGKSTLALQLVQSGWSYLSDDELLLSLNDGAVEARGFRSFFAISEAGTQFKRC
                     FEPLGLKRVELAYPGFLLFISLNGESRSQLGKLTPAETMTRLITACPWATYDRSVAGA
                     NLELLSTLARQSRGFDLSAGRDLLEPGFAASFFSAALCVSLRSLR"
     gene            complement(3253..3414)
                     /locus_tag="VFH96_05505"
     CDS             complement(3253..3414)
                     /locus_tag="VFH96_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973459.1"
                     /translation="MAKQNEIIKEEIADGSGEGSELKKVFVEPAISVPVDVLEATTFF
                     QSATSGVVN"
     gene            complement(3521..4381)
                     /locus_tag="VFH96_05510"
     CDS             complement(3521..4381)
                     /locus_tag="VFH96_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973460.1"
                     /translation="MKRTGFCHYATGITLTIVVCLALSTTSLAQKPATPETDTKQTQK
                     DSKPAETKAAAKLPYSIKTRKSPILNISLKAEKAKMSEVAQELSKQLKVPIFLGPERQ
                     NEIVTLEFSELTLEPALQLMSPVVYVDYEIDTGSGAPPKALGIYLFDTNQGEPPLTTV
                     INGATQSMLIEGNTEDGVEPESEDDKKKLEEQPLRIQFKDNLLSVKAKKQPVALVLLK
                     IGEELGIPVDIQDQNVTTVIDAEISKLPVEDVVRQLSPQIRLFVRADLTHAERSALRI
                     VLAEPPKATQ"
     gene            complement(4479..5609)
                     /locus_tag="VFH96_05515"
     CDS             complement(4479..5609)
                     /locus_tag="VFH96_05515"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6973461.1"
                     /translation="MNADQIRVYPRKSAAELLLGYARTSAGEVDWEYLFQLARRHSIV
                     PLVYVQLQEHASDLVPQPFLNKLKKHYFENSARNTLLTAELCRLINLFRDEDIEAIPY
                     KGPVLALFAYGNVDLRRFVDLDVIVKKSDVLKAREILLKSGYKPSKSLSLDQQEMLLR
                     TQHNMQFSRDNHRLIIELHWEVAPHLFASTVNGERLWQDLITLDLNGTPVKTFSAEDL
                     LFSLCVHGSRHLWERLGWICDIAELIKRQPLDWTALLERAAVSDTERMFLLGVHLAQR
                     LLDAPVPAEVKQRCDADQRLSSLADNIIEHLFNGPTHVPATSREIFKYNIGVRKTLGA
                     RARYFLYMFRPTDRDLSSRSLPPSLSFAYYLTRPFRLFRTKI"
     gene            complement(5635..7434)
                     /gene="lepA"
                     /locus_tag="VFH96_05520"
     CDS             complement(5635..7434)
                     /gene="lepA"
                     /locus_tag="VFH96_05520"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6973462.1"
                     /translation="MDPSRIRNFSIIAHIDHGKSTLADRLLELTGALSEREMENQVLD
                     NMDLERERGITIKAHAVRLDYKAQNGERYVLNLIDTPGHVDFSYEVSRSLSACEGALL
                     IVDASQGVEAQTLANTYLAIENNLELIPVINKIDLPGAEPEKAIEQIEHFIGLETHNA
                     ILTSAKTGQGVSEVLEAIVRDVPPPKGDPNASLKALIFDSWYDSYRGVIVLFRVIDGT
                     IRPGMKIRFFNANRDYQVETLGVNRPTPTPIDHLSVGEVGFLTASIKAVADVQIGDTV
                     TEAARPTKEPFPGYQEIKPMVFAGLYPIDAAQYEELRDALDKLRLNDASFFYEPESSA
                     ALGFGFRCGFLGLLHMEIVQERLEREFNLDLITTAPGVRYVVTTTDGQVLEIDSPAKM
                     PDPGRIAKIEEPVIRAVILTPDEHLGGILPLLEEKRGLQKGFEYITSNRVMLTYELPL
                     NEIVLDFYDRLKSVSRGYASLDYNLAGYWESDLVKLDVLVAGEPVDALSLILHRSTAQ
                     PKGRALAEKMKQLIPRQLFEVPIQAAIGNKVIARETVKALGKNVIAKCYGGDITRKRK
                     LLEKQKEGKKRMKKVGRVEIPQEAFLAVLKVNE"
     gene            complement(7446..7934)
                     /locus_tag="VFH96_05525"
     CDS             complement(7446..7934)
                     /locus_tag="VFH96_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973463.1"
                     /translation="MTRNQKIALGCGGAGCLGLIVVAIAGGLIYYFMRSAATNRNYNF
                     NVSTNRNSNSNDNSDFTTNRNGNDNDNSSNSNSSSTSSSSMSDDDKHKLYQAGLIVGD
                     QELLRRISVKIGIMEDDFTPGNNYQEFVVEQVSWAIRNTEFTNTINTPAKARAYINEH
                     MD"
     gene            complement(7997..8875)
                     /locus_tag="VFH96_05530"
     CDS             complement(7997..8875)
                     /locus_tag="VFH96_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6973464.1"
                     /translation="MASLQQRLPENVPGDFFVDSTCIDCDTCSQLAPTIFRDHGEQCS
                     VYHQPATDDETQLAMMALVSCPTGSIGAIERHDAHLGIDAFPMLLEDNVYFCGFTSED
                     SFGAWSYLIVRPESEGGNVLIDSPRFASQLVKQIETMGGVSQMLLTHKDDVAAHKLFR
                     DRFDCERLMHADDGASRLGVEHVIRGEEPVQLDDDLLVIPTPGHTRGHVVFLYRNKFL
                     FTGDHLAWSPARRTLTAFRSVSWYSWPEQTRSMEKLLSYEYEWVLPGHGDIHRDDART
                     MRDHLARCVDWMKTIK"
     gene            complement(8964..10340)
                     /locus_tag="VFH96_05535"
     CDS             complement(8964..10340)
                     /locus_tag="VFH96_05535"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6973465.1"
                     /translation="MTHQSQPKGTVLVVDDDDAVRSGLFWALTSDYQVLQASSRDEAC
                     TQINNEQIEVVVSDLHLPPHIDDIAEGLALIDVARAQDPPLQVVVITGSDSKRAALEA
                     VRRGAYGFFEKPLDPAELLHIVNQAARMRTLEIENRRLREELSRPPGFAHLTGTSQAL
                     ERVLKQARSVAATSATVLLSGENGTGKEMLARAIHQESPRASGPFVAVSCAALPEALI
                     ESELFGHEKGAFTSATQSHKGRFELADGGTLFLDEIGDLSTAVQVKLLRVLQEREFER
                     VGGTKTLTVDIRLIAATNRDLEKEVAANRFRQDLFFRLNVVPLVLPPLRERPDDIPIL
                     AAHFAAKSAQKYGQTTAELEPALVEVLLEYHWPGNVRELENLIERLVVLSSSSTLGLE
                     FVPEKMLRVLPGTAPGDESTLEGAVEALKRRMIKSALQSEGNKVAAAKKLGISRSYLH
                     RLINEYDL"
     gene            complement(10464..12254)
                     /locus_tag="VFH96_05540"
     CDS             complement(10464..12254)
                     /locus_tag="VFH96_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973466.1"
                     /translation="MRRKPFLTYFCLCAIPLLLLAGLNYWNGLRSVDATTGTIVQSDL
                     NAFNVAIDQELRNRKSDFLRLSMSEEAQYIVSDKEREDVYPQGMFLWLKTLPLLREFE
                     SLTIFNRDRHAFAFRRGESEWTTLENYHGAEFTQPDPHIWETQGNVSLERVDSAGKTV
                     EYSAPIHDEKGTSTIGGLIGVLNLDSMFANAARGLESKSSQLMVMAVDRSGKIVYHSN
                     RSLKDLQVNQALPGFNAIANAMTNNDSGVREFKSPAGSYLAAYSPLPQLNVAVAVARD
                     RASALSSAHRWGIAGIVLALLFAAGAAFLLEQHVQSRSKGIEQVSADVSAIAKGEFDR
                     RILLESSDDFRSLADNINVVTDQLRAQIAREEETRQFESFMRLSAMLTHDLKNAIEGL
                     SLTVGNMERHFDNPQFRTDALKGLTSAANKLKALVARLSRPMTSLSGEHKRPTNVDLI
                     PIIQRVIAITAEPQRSKHSIVTRLPPSLYALADAARVEEVLENLVLNALEAMTDKGGT
                     LTIEAGQTTNGAPTFAVSDTGRGMSRQFIETRLFRPFATTKRTGIGLGLYTCREVIRA
                     SGGTIEVDSVEGAGTTFRVVLPSIPHERRN"
     gene            complement(12291..13136)
                     /locus_tag="VFH96_05545"
     CDS             complement(12291..13136)
                     /locus_tag="VFH96_05545"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET6973467.1"
                     /translation="MKAYDHELGVVCELARVAGAAILEHYEGPLHIKQKNYDDDTEPV
                     TQADTIANDLIVTGLKREFPDDGILAEESVDTKRRLEKSRVWMVDPLDGTNGFIDGNG
                     DFAVQIGLAEDGRCVFGVVYQPLTGVLYRAVQGGGTWIERPQFEPEQAKVSDQKTISE
                     MRLAASRSHRSPRMNKVVMRLGFKDEVQRGSVGIKIGLLVEQQCDVYIHLSPRTKQWD
                     TCAPEVILTEAGGRITDLFGYPLDYNVPDVQNRNGLVASNGISHDQIIETLAPLLNEF
                     GRKRA"
     gene            complement(13147..14619)
                     /locus_tag="VFH96_05550"
     CDS             complement(13147..14619)
                     /locus_tag="VFH96_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET6973468.1"
                     /translation="MSLRTQSSLDVNEDDLQQISSESTKLVCEYLTTISERPVRAENH
                     AGKTLAAIDNELSSDGRSIEQLIGECRTIMDLSRHNGHPRFFGYVASPSTPVGAYADL
                     ISSALNANITCWRSGPAGTEIERLVVRWLGALIDYDRDAKGLLTSGGSMANMIALLIA
                     HRRKSGVDTARHGLWNSGPPMTLYASEEVHLSIAKAADILGFGRDQVRVIACDGRQRM
                     RVDLLRQKIEADRSEGLRPFCVVGSAGTVNTGVVDPLAELANVAAEFDLWFHVDGAYG
                     APGSLDERKKHLFAGLERADSVSLDPHKWLYVPVDAGCLLFHDAEAARAPFSAEDADY
                     IKTHGYADEEAFAFWDYGVELSRRFRALKVWMTLQYYGSRRIAAAIGEDIALAAYLGE
                     LVSNADDFELLAPVELSICCFRYVPHSGMSDEELDRVNERIMALVQKGGRAYLSNATV
                     HGKFALRACITNFRTTKADIEETVAAVREAAEKLVADERG"
     gene            14661..15596
                     /locus_tag="VFH96_05555"
     CDS             14661..15596
                     /locus_tag="VFH96_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6973469.1"
                     /translation="MPAKQSLVLSTIGIGTYLGGADEVTDLSYTRAIVRAVQLGVNVI
                     DTAANYRFQRSERSIGEALKILANEHRVAREELVICTKGGYLPFDGAPPRSVAQYVNE
                     TFVKTGIASFEDFVGGGHCMTPAYLQSQIDQSLRNMGIESIDVYYIHNPESQLPHVSE
                     QEFYARLKRAFERLEENRREGKLVHYGVATWNGFRMPPNSGQHHSLVRMVELAREVGG
                     EQHGFHFIQLPFNMMMPEALAIGNEMLDGEAMPVIEAAQRLGVTVVASASLMQGRIDP
                     QSAIQFVRGVGGVTTALIGMSRVEHVEENLATDLH"
     gene            15596..15928
                     /locus_tag="VFH96_05560"
     CDS             15596..15928
                     /locus_tag="VFH96_05560"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HET6973470.1"
                     /translation="MNTADRYRSFSEFWPFYLSEHSRPATRWLHLVGTTAGIALLIYL
                     VATGRWWLFFLGFVPGYGCAWLAHFLIEKNRPATFKYPLWSFMGDYKMIALMLTGRMG
                     AEVKKLAR"
     gene            15918..16949
                     /locus_tag="VFH96_05565"
     CDS             15918..16949
                     /locus_tag="VFH96_05565"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET6973471.1"
                     /translation="MRGNIPIPEIPFGEDLWLIVLITSVRERRTQQGRLFCDASARNA
                     TGTIALKIWGETLETQKEIKPGLWGITGKLETFQDRSQFVVAEYRPITLEQYREHQQA
                     EPQLPRAYTIDIETLTLPDFRERVGAQLERTMRLGNMRVEQQQRYLEDIAAEEERCYA
                     LGALSATSGRVLSIALHVGSIAGLEFPGLGPEDSEHVFGIDKYGYEEDEKEALQNFLT
                     MMKDFDPDCDELVGHNVIGFDLPFIFQRCLAHSLKARPFVNLGEYNVRGVFDTMHRWW
                     LGAKRHVSLDDVAWALGIESSKTADVEGSKVFDLYQAGRLNLIREYNLNDVRVTRKIY
                     ERMVSSFGR"
     gene            17076..18416
                     /gene="rmuC"
                     /locus_tag="VFH96_05570"
     CDS             17076..18416
                     /gene="rmuC"
                     /locus_tag="VFH96_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET6973472.1"
                     /translation="MEIVFPVSMLALGLSIGAFSVWFITRTKLKYEFDRGRTYGETER
                     AVLQTRLEEERKANQEKITLINNAETKLADAFKALSADALRNNNQSFLDLAKQNLEAF
                     QQNAKGELERRQSAIGDMIKPLKESLEKVDGKIGELEKNRVGAYSELREQVKVLAQSH
                     LQLQSETGNLVKALRVPHVRGRWGEIQLRRVVELAGMLQYCDFVEQETVATEESRIRP
                     DLVVRLPGNRTIVVDSKVPFDAFYESISTTDEEIRASRLKDHARLVRTHIQSLSRKSY
                     WESLQPTPEFVLLFLPGETFYSAALEHDPKLIEDGISQRVIIATPTTLIALLKAVSYG
                     WRQEQMADNAQEVSNLAKTLYDRLRVFTNHFDDIGRNLDRALDAYNKGVRSLESRVLV
                     TARRFKERGAIAGEEIEILEPVDKSARALTRDDGGLFPEENMPLLKARTAKAKS"
     gene            18499..>22032
                     /locus_tag="VFH96_05575"
     CDS             18499..>22032
                     /locus_tag="VFH96_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET6973473.1"
                     /translation="MNEIWLGPLLNDNRALLIERCAKFLANNQSDAFIYLAASYPLLE
                     RVTQELLDGQRTRGVWGELPVYLFRGLVRRVLYSAVDENGHNLPPRIPIDRDELPLKR
                     SLISQLLLQLLANNQLKAFAPLATREGCVNSISTLLGEIQRAARTPAELADILNTRAK
                     DLEKGNGTTRGITQLDFDKEISLIYSNYTELLDGSLFTEEDAEQLRALWILRGMIDQH
                     WVKAPWLANVQLLVIDGFFDFTPVQGEILRQLIPLLPQTIVNLNHDDSNPEIFTPFQE
                     TIGQFESIAKFDQVHSRDYVATGGVLAELRENLFNPLTSSDEVSEEPEALVSEIRYLE
                     CGDRDTEIRTIAREVKRLVLVENYDLAEIALVIRQRATYGSTITRVMLEESLPCNLEL
                     RIDAGDVPANRAALKLLSLLEGLSTEDPQMTRIAEIADLVKSEYFRLSDAEIRVLSTR
                     FDEQHSNLLDGNLNLKNRYRIGFWDADSLENAFAYVGSELSVSAWLARAHKLYTNLPG
                     AAATKELLNIDPGAYDRDRDIADNVENAEIVKLEDKGVEKKRRPSRDVHPAALAWTAL
                     VVQRFSELLHAVPREGKPAQLRAELMRLFDRLGFRDQIAEPAKHSPDEKQLPQVMLNF
                     NALEGLRRAFVAAIKSIELVAGGPRTVRLAMFLQEVRRSLAAQSQIFGAPDRNGLRVL
                     EATDVRGLRFRAVFIAGLIEGGFPLSASRDWIYPHEERDRLREYGLTLEDISPNTLLK
                     EEHYFYQVACRATERLYLTRPLMLGDDSETVASYYIDELRRAIAPRRLETEIIRRDYD
                     GKNLDDASTTTEMKVGLVRQQERHLHRGAKSSLLPQPRIMRLLTLARNDGFLSEAALR
                     RIEIERERAGPYFGMYDGEITDPDLLHLIKLKFGSDFIHSASGLSVYGNCPYRFFAQR
                     VLRLEPRGEAALDLQAIDAGKLLHDILRRFFERHRREPLHKLDRRKLQKELANIADAV
                     FDEHERIVPPLNRQIWKIDREIRKILLDQVLLYELEVQESAEGQQVLPSYFEVAFGGM
                     KSAAKDPDSKEEPLELTRKTFVGEETIKISGQIDRVDVADDDTLIAYDYKLSTGSSRS
                     DIISGRSLQLPIYLEALEKLILPNQPIAGGGYYVIRGGQDRRNKGLYRASQLDYLNLR
                     AKNSVFTDEDWTRIREQVVARIWEFLDGIRAG"
BASE COUNT         4896 a         6203 c         6085 g         4848 t
ORIGIN      
        1 atctcgccca tcgacttgcc tttttcgagg cccttccaga cgaccatcgc cgtctcgttc
       61 agttgatagt atttctttgt gttgagatat acgagtattc cctcgccgac gtcgaagtct
      121 gtggcgacaa cgtgtttgcc tggccttagt tcgttcctga tcatttcagg ggttcgagtt
      181 tcgcttctgt tccaggagcc gataaggctc cgcggcccag cgctcgctca tttcgcgagc
      241 cgtgtcggaa gcgagttttt gttttgcgtc tacgtcggcg acaaacgcgc gcgtgccgga
      301 aacttctttc gagtaaacct cactcgtgcg cgcgatcgtc tcgagctctt cactcgcgag
      361 actgacgttg aaaaagttca atacacttgc gattacgggt acagacagtt gattgtaatt
      421 aagcaacatg ctgttcgcgt tcgcaagctg gtacgcggct gagtaaagac ttccgatcgt
      481 tcgcgcgcac aactcttcga gactcatctc gctcgcatcg ccgattatcg acgacagcac
      541 gcgtcgatcg ttatcgatga gccacggtgg aatgtcgctc atgtttgaga cgattgtttc
      601 caccggatct ctataaagaa aaagccacgg cacgttgggc cagatgcgct tgatgcgttc
      661 gagctgggcg aagctgcaac acgcaaactt gatgaagaga tatcgctcgt tatccgtgcg
      721 acgttggccg agtgcgtgga ccacgctccg caggaaaact gaatagagtg actctttgac
      781 ggggttgtca gcgtcagtga tgaagcgagc tacaagctta tcgatcgcgt ttgcttcgga
      841 gagaacgatc gagttggaga ctgcgcgaca cgcgttggcg acgagcgttg agccgcaacg
      901 tgaggagtga aaaataaatc cggtaggttc gacgctttcc agctgcttct cgagttgcag
      961 cagtacatca aattcagtaa atcgcgcggc tcgattttct cgtctcgcac gctcgactgt
     1021 ttgttgtaag aacggctccg tcaggtcgat acccgacatc tccatccaca tcagtcccgg
     1081 ccgcccgtca acaacaacgg catccaccgg caaccaaccc tttaactccg tgctttgatc
     1141 cgcgttcatc cgtggctaaa tattcctcaa tgccagggaa tcttaccgaa gctttgcaga
     1201 cagaacatac acggcgagcc tgtataaacg tcgggcgtgt gtagtgcggc gagtgcgtcc
     1261 ttccttcgct gcaactgcgc tgtagccaat gtcttaaatt tctcataagc cgacgcgaac
     1321 gactcgacat tcaagtccgc aactacatca agctcctgag ctgctccacg aaagccagac
     1381 agattgcagc atagtgacaa ccgaccacga taatcgatgt tcatgttcac acccgcgagc
     1441 ggcgcgcatg gcgggcgcgt gtcatcgatg ttgtagtaac caacatcgat cccgatattc
     1501 atcttgaaga tgcgcgcgag aatcgcgatc tcctgttcgg ctacacgctg ctcgtcgaga
     1561 ctcaatgccg aattgtctgc gacagcattt gaagtcggca tcacgtgtac gaagttcaac
     1621 gaagcggctc ccatacgcgc cgcgaagatc gcgatctgtt ccagttggtc caccagatcg
     1681 cggcgaatca cgatcttgat cccgaaaggc agcttcgaca tgtagcaacg agtgaacgcg
     1741 cgcaccaaac gatcgaacga tccgtttccg cgccagcgat cgtgatcttc gcgggtgacg
     1801 ccatcgagac taaaagcgac atgcgacaca gcggcgcggt tttcctgtag cgcaggccag
     1861 acgcgctcga agtgccagcc gttggtgaca aaactgacag tcgcacctgc attgccgacg
     1921 atgcgaagtg tttccgcaaa ctcgggatgg agtgttggct cgccaccagt gaaactgata
     1981 cgcgtcacct ttgcggcgtc acgcgcctcg tcaataacac gtcgcagaag atcgaggggg
     2041 aagaattcgg cgtgcgagct gtaaagattc tcgtcggagc gaaagcagta gctacagtgc
     2101 agattacaga tgttggccag ctccacgctc aggcgtcgtg ggccataatc atcaaaggag
     2161 atttcggaaa aagtagccac aaaaaggcac aaaagcacga aaattaagaa agatgctctc
     2221 tgcggccctc tgcgtatctc tgcggccctc tgcgttgaaa cagtccttta acgcagagaa
     2281 cgcagagata cgcagagggc cgcagagaaa aacgacgctg caaagccggg ctctaaaaga
     2341 tcgcggccgg ccgaaagatc aaaaccacgc gactgccggg cgagcgtgga gaggagttcc
     2401 aaatttgcgc cggccacgga tcgatcgtag gtggcccagg ggcaggccgt gatcaagcgc
     2461 gtcatggtct ctgcaggtgt tagtttgccg agttgtgatc gcgattcgcc gttcagactt
     2521 atgaaaagca aaaagccagg ataagccagc tccactcgct tcaatcccaa aggctcaaaa
     2581 cagcgcttga actgtgttcc ggcttcactg atcgcaaaga aactgcgaaa accccgcgcc
     2641 tcaactgcgc catcattcaa gctcaacaac aactcatcat cagataagta cgaccagcct
     2701 gactgcacca attgcaacgc cagggtagac tttccactac ccgatggtcc aatgatcagc
     2761 accgccttcc cgctctgagg ctcaaccaca cccgcgctat gcagatcaaa cagtccgaat
     2821 cgtctcagtg ccgcacaaac cgcaaacgac gcagcccgac caaacaaagg atctcccaac
     2881 cgcggcaact cagtaaacga aactgaaact aagtcgccat tctcgagatg gaccactggc
     2941 ccaccaaact cgagatagag cccggtccca tctgtgtaac acttcccacc gtcggcaatt
     3001 tcgaactggt ccagatcgcg cggaattctc tgcgataagt cttcgcagga gaaactaatc
     3061 tgaatgtcgg gtgatctatc gggaagggag actggagtaa gttgccatcc ggcaaagagg
     3121 tctacgatca ggttacgtag ttcgagactg gaactctcga taaataacaa ccgaccggcg
     3181 accgagtaaa aagcctggga caccggccgg tcaaagacct gaatagattc catgcaaccg
     3241 gaacttctat tactaattga ctacgccgct agttgccgat tgaaagaacg tggttgcctc
     3301 aagcacatca accggaaccg agattgccgg ttcgacaaac accttcttaa gctcgctgcc
     3361 ttcaccactc ccatcagcga tttcttcttt aataatttcg ttttgcttag ccaagttaag
     3421 ctgcctcctc gggtagtttg ctaactatat ggtctcgatc gggcgatggc aacataaaag
     3481 ttgggccagc gtttccgatc gccgatctgg taccgcaatt ttactgagtc gctttgggcg
     3541 gctcggccag aacaattcgt aaagcacttc gttcggcgtg tgtaagatcg gcgcgtacaa
     3601 atagccgaat ttggggggac agctgccgga ctacatcctc gactgggagt tttgagattt
     3661 cggcatcaat taccgtcgtc acattctgat cctgaatgtc gaccggtata ccaagctctt
     3721 caccaatctt cagcagcact aaagccaccg gctgcttctt tgccttaacc gacagtaaat
     3781 tatctttgaa ctgaatacga agcggttgct cctcgagctt cttcttatcg tcttcactct
     3841 ctggctcaac tccgtcttca gtgttgcctt cgatcagcat cgactgcgtc gcgccgttta
     3901 tgaccgtggt caacggcggc tcgccttggt tcgtgtcgaa taagtagatg ccgagcgctt
     3961 tcggcggcgc gccggaaccg gtgtcgatct cgtaatcgac gtaaacaact ggcgacatca
     4021 attgcaaagc cggttcaagc gtcaactcgc tgaactcgag cgtcacaatc tcattttgtc
     4081 gttctggtcc aagaaagatt ggaactttca gttgcttcga aagctcttgc gccacttccg
     4141 acatcttcgc cttttcggct ttcaaagaga tgttcaggat cgggctcttc ctggttttaa
     4201 tggagtaggg cagcttcgcg gctgctttcg tctcggccgg tttggaatct ttttgggttt
     4261 gctttgtgtc agtttcggga gtggcgggct tctgcgcgag tgaggtagtg gatagcgcga
     4321 gacagaccac gatggtcaaa gtaatacccg tcgcatagtg acagaaacct gtgcgtttca
     4381 atttcttgtt aacctttttc cgacgagttg agcggttttg ctccggcgac tggccgcgcc
     4441 tccagttgct ggaaaactaa cacgtgcaaa aagatacgtc aaattttagt acgaaataga
     4501 cgaaagggac gcgtgaggta ataagcaaaa ctcaagctgg gcggcagcga gcgagagctg
     4561 agatcacggt ccgtgggacg aaacatgtaa aggaaataac gagcacgcgc gccgagcgtt
     4621 ttgcgcacgc cgatgttgta tttgaagatc tcgcgcgacg tggccggcac gtgtgttgga
     4681 ccgttgaaaa ggtgttcgat gatgttgtcc gcaagcgacg acaaacgttg atccgcgtca
     4741 caacgttgct tcacttcagc cggcacgggc gcgtcgagta gtcgttgcgc gagatgcacg
     4801 ccgagcagga acatgcgctc ggtgtcggag accgcagcgc gttcgaggag tgcggtccag
     4861 tcgagcggtt gtctcttgat cagttccgca atgtcacaga tccaaccgag ccgttcccaa
     4921 agatgacgcg agccatgcac gcacaacgag aagagcagat cttcagcgga aaaagttttg
     4981 acgggtgtgc cgttgagatc gagtgtgatg agatcctgcc agaggcgttc accgttgaca
     5041 gttgaagcga agagatgtgg ggcgacttcc cagtgcagct cgataataag tcgatgattg
     5101 tcgcgtgaga attgcatgtt gtgttgtgtg cgtagcaaca tttcctgctg gtccagtgaa
     5161 agcgacttgg agggtttata gcctgacttt aggaggatct cgcgtgcttt gaggacgtca
     5221 gatttcttga cgatcacgtc gagatcgacg aagcgacgca agtccacatt gccgtaagcg
     5281 aagagagcga gcaccgggcc tttgtaaggg atcgcctcga tgtcttcatc acgaaataaa
     5341 ttgatcaacc ggcaaagctc ggcggtgagt aatgtgttgc gtgcggagtt ttcgaagtag
     5401 tgctttttga gtttgttgag aaacggctgc ggtacaagat ctgatgcatg ttcctgaagc
     5461 tgaacataga cgaggggaac aatcgaatgc cgccgcgcga gctgaaacaa gtactcccaa
     5521 tcaacctccc ccgcactcgt ccgcgcatag ccaagcaata actcagccgc ggatttacgc
     5581 ggataaacgc ggatctgatc cgcgttcatc tgtgtaaatc cgcggctaaa aatactactc
     5641 attgacctta agaacagcga ggaatgcttc ctgcgggatc tcgacgcggc ccactttctt
     5701 catccgcttt ttgccttctt tctgcttctc gagcagcttg cgtttgcggg tgatgtcgcc
     5761 gccgtaacac ttcgcgatca cgtttttgcc gagcgccttt accgtctcac gcgcgatcac
     5821 tttgtttccg atcgcggcct gaatcggcac ctcgaagagt tgtcgcggaa taagctgctt
     5881 catcttctcc gcgagcgcgc gccctttcgg ctgcgccgtg ctgcgatgca gaatcaacga
     5941 caacgcatca accggctcgc ccgccaccag cacatcgagc ttgacgagat cgctttccca
     6001 gtaacccgcg aggttgtaat cgagcgacgc ataaccacgc gacaccgatt tcaatcgatc
     6061 gtaaaagtcc aacacgatct cgttcagcgg cagctcatac gtgagcatca cgcggttcga
     6121 ggtgatgtac tcaaaacctt tttgcagacc gcgcttctct tcgagcaacg gaagaatccc
     6181 gccgagatgt tcatcgggcg tgagaatcac ggcgcgaatc accggctctt cgatcttcgc
     6241 gatacgtccc ggatccggca tcttcgcggg cgagtcgatc tccagcacct gaccgtccgt
     6301 cgtcgtcacc acgtaacgca cgccgggcgc agtcgtgatc agatcgagat taaactcacg
     6361 ctccaggcgc tcctgcacga tctccatgtg cagcaaaccg agaaagccac agcgaaatcc
     6421 gaatccgagc gcggctgaag attcaggctc gtagaagaac gacgcatcgt tgagtcgcaa
     6481 cttgtcgagc gcatcgcgca gctcttcgta ctgggccgcg tctatcggat acaagccggc
     6541 aaacaccatc ggcttgatct cctggtaacc gggaaacggc tccttcgtcg gtcgcgcagc
     6601 ttcagtcacg gtatcgccga tctgcacgtc agcgacagcc ttgatcgatg cagtgagaaa
     6661 accgacttcg ccgacgctca gatgatcgat cggtgtgggc gtcggtcgat taacgccgag
     6721 cgtttccacc tggtaatcgc gattcgcatt gaagaaacgg atcttcatgc cgggacgaat
     6781 cgtgccgtcg ataacgcgaa acagaacgat cacgccgcga tacgagtcgt accacgaatc
     6841 gaagatcagc gctttgagcg atgcgtttgg atcgcccttc ggtggcggaa cgtcgcgcac
     6901 gatcgcttcg agcacttcgc taacgccctg accggtcttc gcgctcgtga ggatcgcgtt
     6961 gtgagtctcg agaccgatga agtgttcgat ctgttcgatc gctttttccg gctctgcgcc
     7021 cggcaagtcg atcttattga tcactggaat cagctcaagg ttgttttcga tcgcgagata
     7081 agtattcgcg agcgtttgcg cttcgacacc ttgcgaagca tcgacgatca acaacgcacc
     7141 ttcacacgcc gaaagcgatc gcgagacttc gtatgagaaa tcgacgtgac ccggcgtgtc
     7201 gatcaaattg agcacgtagc gctcgccgtt ctgcgctttg taatcaagcc tgacggcgtg
     7261 cgctttgatc gtgatgccgc gctcgcgctc gaggtccatg ttgtcgagca cctggttttc
     7321 catctcacgt tcagacagag ctccggtgag ctcgagcagg cggtcggcga gcgtcgactt
     7381 accgtgatcg atatgagcga tgatcgagaa attgcgaatt cgcgaaggat ccatcttgat
     7441 acaacttaat ccatgtgctc gttaatgtaa gcacgcgctt tggccggcgt attgatcgta
     7501 ttagtgaact cagtgttgcg tatcgcccaa ctaacctgct ccacgacgaa ctcctgatag
     7561 ttattgcccg gtgtgaagtc gtcttccatt attccgatct tgaccgaaat acgccgcagc
     7621 agctcctgat cgccaacgat gagaccagct tgatagagct tgtgcttgtc gtcgtcagac
     7681 attgaagacg aagaagttga tgacgagtta gagttagacg agttgtcgtt atcgttcccg
     7741 ttcctgttcg tcgtgaaatc tgaattgtca ttcgaattgg aattgcgatt tgtgctgacg
     7801 ttgaaattgt aattgcgatt ggttgcggcg cttctcatga agtaataaat cagaccgccg
     7861 gcgatcgcga ccacaatcaa acccaaacag cctgcaccac cacaaccgag agcaatcttc
     7921 tgatttcggg tcattgtttt tcctcagaat agagttagcc acaaaaaggc acaaaaatca
     7981 cagaaaacaa atcagattac ttgatagttt tcatccagtc gacgcagcgc gccaggtgat
     8041 cgcgcatggt tcgagcgtcg tctcgatgaa tatcgccgtg accgggtaac acccattcat
     8101 attcgtaact gagaagtttt tccatcgagc gcgtctgctc gggccacgag taccacgaca
     8161 cgctgcgaaa cgccgtcagc gttcggcgtg caggtgacca ggcgaggtga tcgccggtaa
     8221 aaagaaattt gttgcggtaa agaaagacta cgtgaccgcg cgtgtgtccg ggcgtgggaa
     8281 tcacgagcag atcgtcgtcg agctgcaccg gctcttcacc gcgaatcaca tgctcgacgc
     8341 caaggcgact cgcgccgtcg tcagcatgca tcaatctttc acagtcgaat ctatcgcgaa
     8401 acaacttgtg cgcggcaacg tcgtctttgt gtgtgagcag catttggcta acgccgccca
     8461 tcgtttcgat ctgcttcacg agctggctcg caaatcgtgg cgagtcaatc agcacattgc
     8521 cgccttcact ttcgggacga acgatcaaat aactccaggc gccaaagctg tcttccgacg
     8581 tgaagccgca gaagtaaacg ttgtcttcga gcagcatcgg aaaggcatcg atgccgagat
     8641 gcgcgtcgtg tcgttcgatc gcgccgatcg atccggtcgg acacgagacg agggccatca
     8701 tcgcgagctg cgtttcatcg tctgttgcgg gttggtgata gacgctgcac tgctcgccgt
     8761 gatcgcgaaa gatcgtgggc gcgagttggc tacacgtatc gcaatcgatg cacgtggagt
     8821 caacaaaaaa gtcgccaggg acattctcag gtaaacgttg ttgcaggcta gccacgaaga
     8881 catagtagcc acaaaaaggc acaaaagtca caaaagctga atttgaggtt ttatgctttt
     8941 gtgttttttg tgccttttgt gtactaaaga tcgtactcgt tgattagtcg atgtaagtat
     9001 gatctgctga tgcccagctt ctttgcggcg gcgaccttgt ttccttcaga ctgcagcgcg
     9061 ctcttgatca tgcggcgctt gagggcctcg acggcgcctt ccagagtgct ttcgtcaccg
     9121 ggcgccgttc ccggcagaac gcgcagcatc ttctccggca caaactccag cccgagcgtc
     9181 gagctgctgc tcaacaccac caaccgctcg atcaaattct cgagctcacg cacgtttccc
     9241 ggccagtgat attccaacaa gacttcaacg agggcgggct cgagctccgc tgtcgtctgt
     9301 ccatacttct gcgctgactt cgcggcaaaa tgcgcggcga ggatcggaat gtcgtcagga
     9361 cgctcacgca acggcggcaa cacgagcggt accacattca atcggaaaaa cagatcctgc
     9421 cggaaccgat tcgccgcaac ttccttctcg agatctcgat tcgtcgcagc gatcaatcgg
     9481 atgtcgactg tcaacgtctt cgtgccaccg acgcgctcaa actcgcgctc ctgcaacacc
     9541 cgcaacagct tcacctgcac cgcagtgctc aaatcgccga tctcgtcgag aaaaagtgtg
     9601 ccaccgtcag cgagctcgaa ccggcctttg tgactttgcg tcgctgacgt aaacgcgccc
     9661 ttctcgtgac caaacagctc cgactcgatc aacgcttcag gcagcgccgc acaactcacc
     9721 gccacgaatg gtccactcgc acgcggactc tcctgatgaa tcgcacgcgc gagcatttct
     9781 ttgcccgtgc cattctcgcc actcaacaac accgtcgcgc tcgttgccgc aactgaccgc
     9841 gcctgcttca acacgcgctc gagcgcctgg cttgttcccg tcaagtgtgc gaacccgggc
     9901 ggtcgcgaaa gctcctcgcg cagtcgtcga ttctcgatct ccagtgtccg catgcgcgcg
     9961 gcctggttga cgatgtgcaa caactccgcc ggatcgagcg gcttctcaaa aaatccatac
    10021 gctccacgcc gcaccgcttc gagcgccgcg cgtttcgaat cgctgccggt aatcaccaca
    10081 acttgcagag gcggatcctg cgcccgcgcg acatcgatca acgcgagacc ctcggcgatg
    10141 tcgtcaatgt gtggaggcag gtgcagatcg ctcacgacta cttcgatctg ttcgttgttg
    10201 atttgcgtac aggcttcgtc ccgagacgac gcttgcagca cttgataatc ggacgttaac
    10261 gcccagaaaa gaccactacg cactgcgtcg tcgtcatcga caaccagtac ggtgcctttt
    10321 ggctgagatt ggtgggtcat cttttcctaa aaccgctttc cgcagaacgc acacgtatgc
    10381 ctgaccgttg tttcctgggc aatactgcag aggaacgggc gtaacgggta tgccttgtgg
    10441 aagagctttt gccgcagccc ttcttagtta cgcctctcgt gtggaatcga tggtagcacg
    10501 acgcgaaatg tggttccagc gccttcgact gaatctactt cgatcgtacc gccactagct
    10561 ctgatcactt cacgacaagt gtatagcccg agaccgatgc cggttcgctt ggtcgtggcg
    10621 aatggacgaa agagccgcgt ctcgatgaac tgtcgagaca ttccgcggcc agtgtcgctg
    10681 acggcgaacg tcggcgcgcc gttcgtggtc tggcccgctt cgatcgtgag cgtgccgcct
    10741 ttgtctgtca tcgcttcgag agcattgagg accaggttct cgagcacctc ttcaacccgc
    10801 gcggcatcgg cgagtgcgta aagacttggc ggcagtctcg taacgatcga gtgtttgctg
    10861 cgttgaggtt cggcagtgat tgcgatgacg cgttgaatga tcggaatgag atcgacgttc
    10921 gtgggtcgct tgtgttcgcc gctgagactg gtcatcggac gcgaaagccg agccacgagc
    10981 gctttcagtt tgttagccgc gctggtgagc cctttcaacg catcggtacg aaattgagga
    11041 ttgtcgaaat ggcgctccat gttgccgaca gtcaacgaaa gaccttcgat cgcattcttc
    11101 agatcgtgcg taagcatggc tgaaagacgc atgaacgact caaactgtcg cgtctcttct
    11161 tcgcgtgcga tctgagcgcg caactgatcg gtcacgacgt tgatgttgtc ggcgagactg
    11221 cgaaagtcgt cactcgattc cagcagtatg cgacggtcaa actcgccttt tgcgatcgcg
    11281 gacacgtctg ccgatacctg ttcgataccc ttcgatctgc tttgcacgtg ctgctcgagc
    11341 agaaacgcgg cgcccgcggc gaacaggagt gccaacacga ttccggcgat gccccatcga
    11401 tgcgcgctcg aaagtgctga cgctcgatcg cgcgccaccg ctacggcaac gttcaactgc
    11461 ggcagcggtg agtaagctgc gagatacgag cccgccggtg atttgaattc acgaacgccg
    11521 ctgtcgttgt ttgtcatggc gttcgcgatc gcgttgaagc ctggtagtgc ctggttgact
    11581 tgcagatctt tgagtgaacg atttgagtga tagacgatct ttcccgatcg gtccaccgcc
    11641 atgaccatca attgactcga cttactttcg aggcctcgcg ccgcgttcgc gaacatgctg
    11701 tcgaggttga gcacgccgat gagaccgccg atggtgcttg tgcctttctc gtcgtggatg
    11761 ggtgcggagt attcgacggt tttgcccgcg ctgtcgactc tctcgagtga aacgttaccc
    11821 tgcgtttccc aaatgtgcgg atcgggctgg gtaaactccg ccccgtgata gttctcgagc
    11881 gtggtccatt cgctttcacc gcgccgaaat gcgaatgcgt ggcggtcgcg attgaatatc
    11941 gtgagactct caaactctct cagcagcggc agcgtcttca accacaaaaa cattccctgc
    12001 gggtaaacgt cctcacgctc tttgtcggag acgatgtact gcgcctcttc cgacatgctc
    12061 agccgaagga agtcgctctt gcgattgcgg agctcctggt ctatagcgac attgaaggca
    12121 tttagatcac tctgcacaat tgtgccggtg gttgcatcaa cgctgcgcag tccattccag
    12181 taattgagtc cggcgagcag taagagaggt atggcgcaga gacagaagta ggtgagaaaa
    12241 ggtttgcggc gcatctacga atcctgtttt ctctgtttca cagagaagaa ctaagctcgc
    12301 ttgcggccga attcgttcag cagcggcgcg agcgtttcaa taatctgatc gtgagaaata
    12361 ccgttgctgg ctacgagccc gttgcgattt tgaacgtcag ggacgttgta gtccaaagga
    12421 taaccgaaaa gatcggttat gcgcccaccc gcctccgtca gtatgacttc gggcgcgcac
    12481 gtgtcccact gcttcgtgcg cggcgacaag tggatgtaaa cgtcgcactg ttgctcgacc
    12541 agcaggccga tcttgatccc gaccgagcca cgttgtactt cgtctttgaa gccgagtcgc
    12601 atgacgactt tgttcatccg cgggctgcga tgagaacggc tcgcggccag acgcatctcg
    12661 gaaatggttt tctgatcgga aacttttgct tgttcgggct cgaactgggg ccgctcgatc
    12721 catgttccgc cgccctgaac ggcgcgatag agcacgccag tcagcggttg atagaccaca
    12781 ccgaaaacgc atcgaccgtc ttcggcgagt ccgatctgga ctgcgaaatc gccgttgccg
    12841 tcgatgaagc cgtttgtgcc gtcgagcgga tcgaccatcc acacgcgcga tttttcaagt
    12901 ctgcgttttg tatcgacaga ctcttccgcg aggatgccgt catcgggaaa ttcgcgtttc
    12961 aagccggtga cgatcaggtc gttggcgatc gtgtcggcct gggtgacggg ctccgtgtcg
    13021 tcgtcgtagt ttttctgttt gatatgaagc ggaccttcgt agtgctcgag gatcgcagcc
    13081 cccgccaccc gcgccagctc acacacaaca cccagttcat gatcgtatgc tttcatccgc
    13141 gtgtttttat ccgcgttcat ccgcgaccag cttttctgct gcctctctca cggctgcgac
    13201 agtttcctcg atatcggcct tcgtcgtgcg aaagttcgtg atgcacgcac gcagcgcgaa
    13261 tttcccatgc accgtcgcat tcgacaaata cgcgcggcca cccttctgca ccaatgccat
    13321 gatgcgttcg ttaactcgat ccaactcctc atcgctcatt ccgctatgag gcacgtatcg
    13381 aaaacaacaa atactcaatt cgaccggcgc cagcagctcg aaatcatcag cattagaaac
    13441 cagctcgcca agatacgcgg cgagcgcgat gtcttcaccg atcgccgcag cgatgcgacg
    13501 actaccgtaa tactgcaacg tcatccaaac cttcagcgcg cggaaacgtc gcgacagctc
    13561 cacgccataa tcccaaaacg cgaaagcctc ctcgtcagcg tatccatgcg tcttgatgta
    13621 gtcagcgtct tccgcgctga atggcgcgcg cgccgcttca gcatcatgaa acagcaaaca
    13681 gcctgcatcg accggaacat aaagccactt gtgcggatcg agtgagaccg aatcagcgcg
    13741 ctcgagaccc gcgaacaggt gtttcttcct ttcatccaac gagccgggcg cgccgtaagc
    13801 gccatcaacg tgaaaccaca gatcgaactc agccgcaaca ttcgccaatt ctgctagcgg
    13861 atcaacgaca ccggtattga cagttcccgc actgccgacg acgcaaaacg gccgcaagcc
    13921 ctcgcttcga tcggcctcaa tcttttgacg caacagatcg acgcgcattc gctgccgtcc
    13981 atcgcacgcg atcacgcgca cttgatcgcg tccgaagccg agaatatctg cagctttcgc
    14041 gatcgacagg tgaacttctt cagacgcgta gagcgtcatc ggtgggccgg agttccaaag
    14101 tccatgtcgc gcggtgtcga cgcccgactt acgacgatgc gcgatcagca gtgcgatcat
    14161 gtttgccatc gagccgccgc tcgttaacag tcccttcgcg tcgcgatcgt aatcgatgag
    14221 cgcgccgagc cagcgcacga ccaatcgttc gatctcggtg cccgccggac cggaacgcca
    14281 gcacgtgatg tttgcattga gagcagacga gatcagatct gcgtaagcgc cgaccggcgt
    14341 ggatggcgaa gcgacgtaac cgaagaatcg cgggtggccg ttgtgccggc tgagatccat
    14401 gatcgtgcgg cactcgccga tcagttgctc gatcgatcgc ccgtcggacg atagctcgtt
    14461 gtcgatcgcc gcgagtgttt tgccggcgtg attttccgca cgcacggggc gctcggagat
    14521 ggttgtcaga tactcgcaga ccagtttcgt cgactcggat gaaatctgct ggaggtcgtc
    14581 ttcgttgacg tcaagcgaag actgcgttcg taatgacatt gtggtcacaa gctaacatat
    14641 cctgttaaca ttcgctcgct atgccagcta aacaaagtct cgtactttct acgatcggta
    14701 tcggcacgta tctcggcggc gcggatgaag tcaccgatct cagctacacg cgcgcgatcg
    14761 ttcgtgcagt gcagcttggc gtcaatgtga tcgacaccgc ggccaattat cgatttcaac
    14821 gaagcgagcg ttccatcggc gaagcgctga agatacttgc gaacgagcac cgcgtcgcgc
    14881 gtgaagagct tgtgatctgc acgaagggtg gttatctgcc gttcgacggc gcgccgccgc
    14941 gaagtgtggc ccagtatgtg aatgagactt tcgtgaagac gggtattgcc tcatttgaag
    15001 acttcgtcgg gggcggtcat tgtatgacgc cggcttatct tcagagccag atcgatcagt
    15061 ctctgcgaaa catggggatc gagtctatcg acgtttatta cattcacaat cctgagtcgc
    15121 agttaccgca cgtctccgag caggagtttt acgcgcgatt gaagcgggcg ttcgagcgac
    15181 tcgaagagaa tcgccgggaa ggcaagctgg tgcattacgg tgtagcgacg tggaatggat
    15241 ttcgtatgcc gccgaattcg gggcagcatc attcgctcgt gcggatggtc gagctggcgc
    15301 gtgaggttgg tggcgagcag cacggctttc acttcattca actgccgttc aacatgatga
    15361 tgccggaagc gctggcgatt ggaaacgaga tgctcgatgg cgaagcgatg ccggtcattg
    15421 aagcagcgca gcggttgggt gtgacggtgg ttgcgagtgc atcgctgatg caggggcgga
    15481 tcgatccgca gagtgcgatt caattcgtgc gcggtgtggg aggagttaca acggcgctga
    15541 tcgggatgag tcgcgtagaa catgtggaag aaaacttagc cacggatctt cactgatgaa
    15601 caccgcggat cgatacagat cgttctctga gttttggccg ttttaccttt cagagcacag
    15661 ccggccggcg acgcgttggc tgcatttggt tgggactact gcgggaattg cgttgttgat
    15721 ctatttggtg gcgacgggaa ggtggtggct gttctttctg ggttttgtgc cgggttacgg
    15781 ctgcgcgtgg ctggcccact tcctgatcga aaagaacagg cccgccacct tcaaataccc
    15841 gctctggtca ttcatgggcg actacaaaat gattgcactg atgctcaccg gtcgcatggg
    15901 cgccgaggtc aaaaagcttg cgcggtaaca tccccatccc cgagattcca ttcggcgaag
    15961 acctctggtt gatcgtgctc atcacttcgg tccgcgagcg ccgcacgcaa cagggccgtc
    16021 tcttctgcga cgcctccgcc cgcaacgcca ccggcacgat cgcgctgaag atctggggcg
    16081 agacactcga aactcagaaa gagatcaaac caggtctctg gggtatcacc ggcaaactcg
    16141 aaacatttca ggatcgctcg cagttcgtgg tggcggagta tcgaccgatc acgctcgaac
    16201 aatatcgcga gcaccagcaa gccgagccgc agcttccacg cgcctacacc atcgacatcg
    16261 aaactctcac cctccccgac tttcgcgaac gagtcggtgc gcaactcgag cgcacgatgc
    16321 gacttggcaa catgcgcgtc gagcaacagc aacgctacct cgaagacatc gctgcggagg
    16381 aagaacgttg ctacgcgctc ggtgcgttga gtgctacgtc gggtcgcgtg ttgtcgatcg
    16441 cgctgcatgt tggttcgatt gcgggtttgg agtttcccgg gctcggtccg gaggacagcg
    16501 agcacgtgtt tggtatcgac aagtacggtt atgaagagga cgagaaggaa gcgctgcaaa
    16561 atttcctgac gatgatgaag gacttcgatc cggattgtga cgagctggta ggacacaacg
    16621 tcatcggatt cgatctgccg tttatctttc aacgctgcct ggcgcactcc ctcaaggcgc
    16681 ggccgtttgt gaatcttggt gaatataacg tgcgcggtgt tttcgacacg atgcatcgct
    16741 ggtggctcgg ggcgaagcgg cacgtcagtc tcgacgatgt cgcgtgggcg ctcgggatcg
    16801 agtcgagcaa gactgcggac gtcgaaggca gcaaggtatt cgatctctac caggcggggc
    16861 gactcaatct catccgcgag tacaacctca acgacgtccg cgtcacgcgc aagatctacg
    16921 agcgcatggt ctcgagcttc ggaagatagc atccacagat tgcgcagatt acacagaaaa
    16981 aaatctgtgt aatctgtgga taaaaagccc agatttagtg aaggcgcgcg ttttaaatcg
    17041 catatcttgt gctttttgct gctagaatgc cgcccatgga aatcgtcttc cctgtgtcga
    17101 tgctggcgct tggcctcagc atcggcgctt tttccgtttg gttcatcaca cggacaaaac
    17161 tgaaatacga atttgaccgc ggtcgcacgt acggtgaaac agagcgcgcc gtgctgcaga
    17221 cccgtctcga agaagaacgc aaggccaacc aggaaaagat cacgctcatc aacaacgccg
    17281 aaaccaagct cgccgacgca ttcaaagcgc tctcagccga tgcgctccgc aacaacaacc
    17341 agtcgtttct cgatctcgcg aaacaaaacc ttgaggcatt tcaacaaaac gcgaaaggcg
    17401 agctcgagcg acgccagagc gctatcggcg atatgatcaa gccgctcaaa gagtctcttg
    17461 aaaaagtcga cggcaagatc ggtgagcttg aaaagaaccg cgtcggcgcg tattcagagt
    17521 tgcgcgagca ggtgaaggtg ctcgcgcaat cgcatttgca actccagtcg gaaacaggaa
    17581 acctcgttaa agcgttgcgt gtgccgcacg ttcgcggccg ctggggcgag attcagttgc
    17641 gccgcgtcgt cgagcttgcg ggcatgctgc aatactgcga cttcgtcgaa caggaaacag
    17701 tcgcaacgga agagagtcgc attcgtcctg atctggttgt gcgtctgccc ggtaatcgca
    17761 ccatcgtcgt cgattcgaaa gttccgtttg atgcgttcta cgaatcgatc tcgacgactg
    17821 atgaagagat tcgcgcgagc cgtttgaagg atcatgcgcg acttgtcaga acacacatcc
    17881 agtcgctgag tcgcaagtct tactgggaat cattgcaacc cacaccggaa tttgtcctgt
    17941 tgtttcttcc cggcgaaacg ttttatagtg cggcgctcga acacgatccg aaactgatcg
    18001 aagatggcat tagccaacga gtgatcatcg cgacgccgac gacgctgatt gcattgctga
    18061 aagcggtgtc gtacggttgg cggcaggaac agatggccga caacgcgcag gaagtgagta
    18121 acctggcgaa gacgctttac gatcgactgc gcgtgtttac aaatcacttc gacgacatcg
    18181 gcagaaacct cgaccgtgcc ctcgacgcct acaacaaagg cgtgcgctca ctcgagtcgc
    18241 gcgtgctcgt cacggcgcgg cgcttcaaag agcgtggcgc gattgccggc gaagagatcg
    18301 agatactcga gccagtcgat aaatccgcgc gagcgctgac ccgagatgat ggcggactgt
    18361 tccccgaaga aaacatgccc ctgctgaaag cccgaacggc caaagctaaa agctaagaag
    18421 gatccgcaga ttacacagat cacacagatt ttattgctga tctgtgaaat ctgtgtaatc
    18481 tgtggttaaa aaggttttgt gaacgagatc tggttaggcc cgctgctcaa cgacaaccgc
    18541 gcgctactca tcgagcgctg cgctaaattc ctagccaaca accagagcga cgcgtttatc
    18601 tatctcgcgg cgtcgtatcc gctgctcgaa cgagtcaccc aggagctgct cgacggacag
    18661 cgcacgcgcg gcgtttgggg cgagcttccg gtgtatctgt ttcgcggcct cgtgcgtcgc
    18721 gtgctctact cggcggttga cgagaacggt cacaacctgc cgccacgcat tccgatcgat
    18781 cgcgacgagc tgccgttgaa gcgcagtttg atctcgcaac tgctgctgca actgctcgcc
    18841 aacaaccagc taaaagcatt cgcgccactc gccacccgcg aaggttgcgt caacagcatt
    18901 tccaccctcc tcggcgagat ccagcgtgcg gcgcgaacac ccgccgagct cgccgacatc
    18961 ctcaacacgc gcgcgaaaga tctcgaaaaa ggaaacggca ccacgcgcgg gatcacgcag
    19021 ctcgatttcg acaaagagat ctcgctcatc tattcaaact acaccgagct tctcgacggc
    19081 agtttgttca ccgaagaaga cgccgagcag cttcgtgcgc tctggatcct gcgcggcatg
    19141 atcgatcagc actgggtaaa agcgccgtgg ctcgcgaacg tgcagctctt agtcatcgac
    19201 ggcttcttcg atttcactcc ggtgcaaggc gagatcctgc gacagttgat ccccctcctg
    19261 ccgcaaacga tcgtgaatct caaccacgac gacagcaatc ccgagatctt cacgccattt
    19321 caggagacga tcggacaatt cgaaagcatc gcgaagtttg atcaggtaca cagtcgcgat
    19381 tacgtggcca cgggcggcgt cctcgctgag ctgcgcgaga accttttcaa cccgctcacc
    19441 tcttcggatg aagtctcaga agagccggaa gcgctcgtga gtgagattcg ttacctcgaa
    19501 tgcggcgatc gcgatactga aatccgcacg atcgcgcgcg aagttaaacg tctcgtgttg
    19561 gtcgagaatt acgatctcgc cgagatcgcg ctcgtcattc gccagcgcgc gacgtatgga
    19621 tcaacgatca cgcgcgtcat gttggaggag tcgctgccgt gcaatctcga gctgcgcatc
    19681 gacgccggcg acgttccagc aaatcgcgcc gcgctgaagc tcctctcgtt gcttgagggt
    19741 ttgtcgacgg aagatccgca gatgacacgg attgctgaga tcgcagacct ggtcaagtcg
    19801 gaatatttcc ggctcagcga tgccgagata cgcgtgttgt cgactcgttt cgacgagcaa
    19861 cactcgaatc tgctcgacgg caacttgaat ctgaagaatc gctatcgcat cggtttttgg
    19921 gacgctgact cgctcgagaa tgcatttgcg tacgtcggca gcgagctgag cgtgagcgca
    19981 tggctggcgc gcgcacacaa gttgtacacg aatctgcccg gcgccgccgc cacgaaagag
    20041 ctgctgaaca tcgatcccgg cgcctacgat cgcgatcgag acatcgccga caacgtcgag
    20101 aacgcggaaa tcgtgaagct cgaagacaaa ggcgtcgaga agaagcgacg tccttcgcgc
    20161 gacgttcatc cggcggcgct cgcgtggacc gcgctcgtgg tccaaaggtt ttccgagctg
    20221 ctgcacgccg tgccgcgcga aggcaagccg gcgcagttgc gcgctgagct gatgcgattg
    20281 ttcgatcgac tcggctttcg cgatcaaatc gccgagcccg caaagcattc ccccgacgag
    20341 aagcaactgc cgcaagtgat gctcaatttc aacgcgcttg aaggattgcg gcgggccttc
    20401 gtcgcggcga tcaagagcat cgagctcgtt gccggtggtc caagaacggt gcggctcgca
    20461 atgtttttac aggaagtgcg ccgttcactc gccgcgcagt cgcagatctt cggcgcgccc
    20521 gatcgcaacg gcttgcgtgt gctcgaagcc actgacgtgc gcggcctgcg ctttcgtgct
    20581 gtcttcatcg cgggattgat agaaggcggc tttccattga gcgcgtcacg cgattggatc
    20641 tacccgcacg aagagcggga ccggctgcga gagtacggcc tcacacttga agatatctct
    20701 cctaacactc tgctaaagga ggaacactat ttctaccagg tcgcatgtcg cgcgactgag
    20761 cgtttgtatc tcacgcggcc gctgatgctc ggagacgatt cggaaacggt cgcttcttac
    20821 tatatagacg agctgcgacg cgcgattgcg ccacgccggc tcgaaaccga gatcatccgc
    20881 cgcgattacg acggcaagaa cctcgacgat gcctcgacca cgaccgagat gaaagtcggt
    20941 ctcgtgcgcc aacaggaacg ccatctgcac cgcggcgcca agtccagcct gttgccgcag
    21001 ccacgcatca tgcgattgtt gacgctcgcg cgtaacgacg gttttctgag tgaggctgcg
    21061 ttgcgtcgca tcgagatcga gcgtgaacgg gctggtccgt actttggaat gtacgacggc
    21121 gagatcaccg atccggatct gctgcatctg atcaagctga aattcggatc cgattttatt
    21181 cacagcgcca gcggtctcag cgtttacggc aattgtcctt atcgcttctt tgcgcagcgc
    21241 gtgttgcgac tcgagccgcg cggtgaagcg gcgctcgact tgcaggcgat cgatgccggc
    21301 aagcttttgc acgacattct gcggcgcttt tttgagcgtc atcggcgcga accgttgcac
    21361 aaactcgatc gccggaagtt gcaaaaagag ctcgcgaaca ttgctgacgc ggtgtttgac
    21421 gagcatgagc gcatcgtgcc gccgctcaac cgccagatct ggaagatcga tcgcgagatt
    21481 cgcaagatac ttttggacca ggtgttgttg tacgagctgg aagtgcagga gtcggcggaa
    21541 ggccagcagg tgttgccgtc gtattttgaa gtcgctttcg gcggcatgaa atcggcggcg
    21601 aaagatccgg attcgaaaga agagccgctc gagctgacgc gcaaaacgtt cgtcggtgaa
    21661 gagacgatca agatcagcgg ccagatcgat cgtgtcgacg ttgctgacga cgacacgttg
    21721 attgcttacg actacaagct ctcgaccggt tcgagtcgca gcgacatcat ttccggtcgt
    21781 agtctgcaac tcccgatcta tctcgaagcg cttgagaaac tgatcctgcc gaaccagcca
    21841 atcgcaggcg gtggttacta cgtgattcgc ggtggtcagg atcgacgcaa taaaggtttg
    21901 tatcgcgcga gccagttgga ctacctgaac ctgcgcgcga agaactcggt gttcaccgat
    21961 gaagactgga caaggatccg cgagcaagtc gtcgcgcgta tctgggaatt tctcgacggc
    22021 atccgcgccg ga
//
LOCUS       DASSFQ010000162         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_75141, whole genome shotgun sequence.
ACCESSION   DASSFQ010000162 DASSFQ010000000
VERSION     DASSFQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75141"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFH96_05580"
     CDS             <1..341
                     /locus_tag="VFH96_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973474.1"
                     /translation="RVFLLGFSQSCALNYRFAFTHADRLRGVVGICGGIPGDWESSPT
                     YRHAEIDVFHLTGTRDEFYTPERVRDYERQLKTRARSVQFKSYDATHEIVPEMRSDVV
                     QWLTKTIDEH"
     gene            331..1758
                     /locus_tag="VFH96_05585"
     CDS             331..1758
                     /locus_tag="VFH96_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6973475.1"
                     /translation="MNTENTNTSPTVSELERDVEARSAVFKKELGLFDLVLTQVVFVV
                     GTFWVGWAARLGSQQSAFWILAIVTFYVPLAVAVIFLNRLMPYEGGLYQWSKLAFNDF
                     VAFMVGWNLWVFAITILAGTGLVVTTNLSYAAGPPAAWIRDSRIMIMLISVVLVSAMV
                     VAATRGLALGKWVHNVGGIVHMITFAVLILLPLFALSRALGGPYEPFAVQLPTFSEYN
                     VNVGSKLALGALTGFEYIAIAAGETRSPAKNIARSVVISAPIIALMFILGTSSVVAFI
                     PRDHIDVIGPIPQVLSIGFGSLGWVGAIGSIAILGIAVRTIALMSLYFVGNTRLPMVA
                     GWDRLLPAWFSRLHATYKTPINSILFVGAVTLVIGAASLSGVHAHEAFQLIDNAGGVF
                     YALTYLVLFAIPLFGMKALGVKAPWWLKIACASGFIVTLIYIRYAIVPITNVEDLRSF
                     GLKIIVTVVVANVVGVGIYVLRTRK"
     gene            1736..>2826
                     /gene="hutI"
                     /locus_tag="VFH96_05590"
     CDS             1736..>2826
                     /gene="hutI"
                     /locus_tag="VFH96_05590"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET6973476.1"
                     /translation="MSSEQESKTLAVVNCLQLVTLAGPERPRVGPELRELGVIPNGGM
                     FVRDGVIESVGSRDEIELLIDSDTEVVDALGKVVLPGFVDAHTHPVFGGTRVSEFEER
                     SQGATYEEIAARGGGIQSTVNATRAMYEEVLTYECWTYSDWFLRSGTTTIEAKSGYGL
                     SLEDELKILRVIKRLGSETSLRYVPTFLGAHSVPAEYRSRRDEYVSLLIEEMLPAVQE
                     EKLAEFCDVFCEQKVFTTDEAWKILSAARCHGMGLRIHADQLSLSGGAQLAAELGTVT
                     ADHLEHTDAAGIAALKKGDVQPVLLPGSVYALGSTRYPAAREMIEAGLAIVLATDFNP
                     GSSPTPSIPMILSLASTHMKMTPAEGITAV"
BASE COUNT          548 a          742 c          843 g          693 t
ORIGIN      
        1 cgcgtgtttt cctgctcggc ttctcgcagt cgtgcgcgct caactatcgc ttcgctttca
       61 ctcatgccga tcgtctgcgc ggagtggttg gtatttgtgg cggcattccg ggcgactggg
      121 aatcaagtcc gacatatcgt cacgcagaaa tcgacgtgtt tcatctcacc ggtacgcgcg
      181 acgagttcta cacacccgag cgcgtgcgcg attacgagcg ccaactaaag acacgcgcgc
      241 gttccgtgca gttcaagagt tatgacgcga cgcatgaaat tgttcccgaa atgcgctctg
      301 atgtggtcca atggctgacc aaaaccatcg atgaacactg aaaacactaa tacctcgccg
      361 accgtcagtg agctcgagcg tgatgttgaa gctcggagtg cagtttttaa aaaagagctc
      421 ggattatttg atctcgtgct gactcaggtt gttttcgtcg tgggaacatt ctgggttggt
      481 tgggccgcgc ggttaggtag ccagcaaagc gcattctgga tcctcgcgat cgtcacgttt
      541 tacgtgccac tcgcagtcgc cgtgatcttt ctcaatcgcc tgatgccata cgagggcggg
      601 ctgtatcaat ggtccaaact ggcgttcaac gatttcgtcg cgttcatggt cggctggaac
      661 ctctgggttt ttgccatcac gattctggca ggcacgggct tggtggtgac tacaaatctt
      721 tcttacgcgg cgggaccacc ggcggcatgg attagagaca gccggataat gatcatgctg
      781 atcagcgttg tcctcgtgtc ggctatggta gtcgctgcca cccgcggcct ggcgcttggc
      841 aagtgggttc acaacgttgg aggcatcgtg cacatgatta cctttgccgt tttgattttg
      901 ttgccgctct tcgcgctatc gcgcgcactc ggaggcccgt acgaaccgtt tgcggtccag
      961 cttccaacct tttctgagta caacgtcaac gtcggcagca agctcgcact cggcgcgttg
     1021 acgggcttcg aatacattgc cattgccgcg ggcgaaacac gctcaccggc aaagaacatc
     1081 gcgcgttcag tggtgatctc ggcgcccatt atcgcgctca tgttcatcct gggcacgagc
     1141 tcggtagtgg catttattcc gcgcgatcac atcgatgtga ttggaccaat tccgcaggtg
     1201 ctttcgatcg ggtttggatc gctcggttgg gtcggagcta taggttcgat cgcgatcctc
     1261 ggtatcgccg ttcgcacaat cgccctgatg agtctctatt tcgttggcaa cacgcgtttg
     1321 ccgatggtcg cgggctggga ccggttgctg ccggcgtggt ttagccgttt gcacgcgacg
     1381 tacaagacgc caatcaactc gattctattt gtcggcgcag tgacgctggt gatcggtgca
     1441 gcaagcctga gtggtgtaca cgcgcacgaa gcatttcaac tcatcgacaa tgccggtgga
     1501 gttttttatg cgttgaccta cctggtcctg tttgcgattc cgctgtttgg aatgaaggcg
     1561 ctgggcgtca aagcgccgtg gtggctcaag atcgcatgcg cgtccgggtt tattgtcact
     1621 ctgatctata ttcgttatgc gatcgttcca atcacgaatg tggaagatct tcgatcgttt
     1681 ggtttgaaga tcatcgttac ggtggttgta gcaaatgtcg tcggcgtcgg gatctatgtc
     1741 ctcagaacaa gaaagtaaaa cgttagcagt tgttaactgt ttgcagctcg tgacgctggc
     1801 tgggccggag cgcccgcgcg tcggtcccga gttgcgcgag ctcggcgtca ttccgaacgg
     1861 cgggatgttt gttcgtgatg gagtgattga gagcgttggc agtcgagatg agatcgagct
     1921 tttgatcgac agcgatacgg aagtcgtgga tgcgctcggg aaggttgtgt taccggggtt
     1981 tgttgatgcg cacacgcatc cggtcttcgg cggcacgcgc gtcagtgagt ttgaagagcg
     2041 cagccaaggc gcgacgtacg aagagatcgc ggcgcgtggt ggtggaattc agtcgacggt
     2101 caacgcgacg agggcgatgt atgaagaagt gttgacgtat gagtgctgga cttacagcga
     2161 ctggtttctc cgctccggca caacgacgat cgaagcgaag tccggctacg gcctttcact
     2221 cgaagacgag ctcaagatcc tgcgcgtcat caaacgtctc ggcagcgaga cttctttgcg
     2281 ttacgtgccg actttcctcg gcgcacacag cgtccccgcc gaatatcgat cgcgacgtga
     2341 cgagtatgtt tcgctgctca ttgaagagat gctgccggca gttcaggagg agaagctcgc
     2401 ggagttttgc gatgtctttt gcgagcagaa agtgttcacg accgacgaag cgtggaagat
     2461 tctttccgcg gcgcgttgtc acggaatggg tttgcgaata catgcggatc agttgtcgct
     2521 ttcaggcggc gcacaactgg cggcagagct ggggacagta actgccgatc atctcgagca
     2581 cacggacgcc gcaggcatcg ccgcattgaa aaagggcgat gtacagcccg ttttgttgcc
     2641 aggctcagtg tatgcactcg gatcgacacg ctatcccgcc gcgcgcgaga tgatcgaggc
     2701 tggcttggct atcgtgcttg cgactgactt caatcccggt tcttctccga caccatcgat
     2761 tccgatgatc ctttcgctcg cctcaacaca catgaagatg acgcccgctg aagggatcac
     2821 ggccgt
//
LOCUS       DASSFQ010000163         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_517489, whole genome shotgun sequence.
ACCESSION   DASSFQ010000163 DASSFQ010000000
VERSION     DASSFQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517489"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1411)
                     /locus_tag="VFH96_05595"
     CDS             complement(77..1411)
                     /locus_tag="VFH96_05595"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6973477.1"
                     /translation="MTDKNDKREIFGWKMYDWANSAFYTTVVGALFGPYLTRLAQAAV
                     GENGVVMDLGPFGEVTAKSLPTLCVSISVGLQVFLLPILGALGDYSNLKKRLMALFCY
                     LGVAANCLLFFVQGNLYLAGGVLFIMANICFGASIVFYNAFLPEITTDDQADKVSSRG
                     YAYGYLGGAILLVFNLFLVLQAERLGISTGLAVRLSLLSAGVWWGAFSILTFTLLKSR
                     PAKKNLPPGKTYISAGFSELRATVKELRRLPLTARYLLGYLIYNDGIQTVIVASSTFL
                     EQELFPGQGNPTFLLEIFLMVQFVAVVGALLFERLAYLINTKNAIIVSLVIWSGIVIY
                     ANRFLDTVPEAWVMAGVIAIVLGGSQALSRSLFSKMIPAGKEASFFGLYEVSERGTSW
                     MGPLLFSLVVSRTGSYRLALLSLIFFFVVGLVVLWFTDTDRAIRESQQVAEQ"
     gene            complement(1448..2449)
                     /locus_tag="VFH96_05600"
     CDS             complement(1448..2449)
                     /locus_tag="VFH96_05600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6973478.1"
                     /translation="MSTERIAYGELVGSSDSIRKIYTLIEQVAPSSASVLITGESGTG
                     KELVARTIHQLSPRREKPFVAINCSAIPETLMESELFGHEKGAFTGAASRRQGCFEIA
                     DSGTLLLDEIGEMPALLQAKLLRVIEERAVRRLGSRKEIEVDVRLLAATNRPPQQAVS
                     EGKLRSDLLYRLNVFGIHLPSLRERKEDLPILAQHLVTQLAEKHGRPARYLSPAALDA
                     LQFHSWPGNVRELRNVIERAVIICSGEQIERHYFAPYPIDQRERMRSEDMLSLPVGTP
                     LEEVERQMIMRTLQKTKNNKTRAAELLGISLKTLHNKLNLYRERGLLQDVSTDYTEQ"
     gene            complement(2485..3294)
                     /locus_tag="VFH96_05605"
     CDS             complement(2485..3294)
                     /locus_tag="VFH96_05605"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET6973479.1"
                     /translation="MNRIPLEGRTVIVTRAASQAAELTTILESYGAKVIVCPTIEIRE
                     PDNYDRLDEALDHLYGYDWLIFTSTNGVEFFLKRLIDRGHQVSDLDEIKVCAIGQRTA
                     DKLHEAHVHVDLVPSQSTAEGVFTALSEFVGGDEHLRGLNILLPRAVVGRELLPEALQ
                     KAGARVDVVTTYQTVLPDNIDRGKLAAMLAGSGDCIAFTSPSTIKNLAKLFDTHDLGK
                     TLPGMVVACIGSVTAEAAAEYGLRIDIQPDQFTTKDLAQAIAAYFSAADLH"
     gene            complement(3363..4277)
                     /gene="hemC"
                     /locus_tag="VFH96_05610"
     CDS             complement(3363..4277)
                     /gene="hemC"
                     /locus_tag="VFH96_05610"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET6973480.1"
                     /translation="MSRPLIIGSRGSKLALWQAEQARARLQALNPQLDVHIEIVKTTG
                     DVKNDPLSVIGGKGVFTKELEDALLDGRIDLAVHSLKDLPTILPEGLSISAICEREDA
                     RDALVLRAGSESGSLLNLPEGAVVGTSSQRRLAQLKALRSDVVVKDLRGNVDTRIRKL
                     DEGQYDAVILASAGLVRLGLQERISARIAIGEMLPAVGQGAIAIETRMDNEFAVQNTS
                     RLDHRETRVACLAERAFLRGLGGGCQFPIAAHAILDGDSLKLDGLVAKPDGSEILRGA
                     SSGSPQDPEGLGSSLAVHLIDRGACALL"
BASE COUNT          993 a         1210 c         1233 g          919 t
ORIGIN      
        1 tctgtcgaat gtaagatgtt caccgaacgg gcggccacgg agagccgccc ctacagttgc
       61 tattagaggt cgtcaattat tgttcggcga cttgttgtga ttcacggatc gcacggtcag
      121 tatccgtgaa ccacaacacg accagcccca ccacgaagaa gaagatcaac gacagcaacg
      181 ccaaacgata agaccccgtc cgcgatacga cgaggctgaa caacaacggc cccatccatg
      241 aggtgcctct ctccgagact tcatacaacc cgaagaacga agcttctttg cctgccggaa
      301 tcatcttcga gaacagcgat cgcgaaagcg cctgcgatcc gccgagcacg atcgcgataa
      361 cacccgccat cacccacgct tccggaactg tatcgaggaa ccggttcgca tagatcacga
      421 tgcctgacca gatcacgagg gaaacaatga ttgcgttctt cgtattgatc agataagcaa
      481 gtcgctcgaa gagaagcgcg cctaccaccg cgacgaattg gaccatcagg aagatctcga
      541 gcagaaacgt cggattgcct tgtcccggaa aaagctcctg ctcgagaaac gtgctcgacg
      601 cgacgatcac tgtctgaatc ccgtcgttgt agatcaggta gccgagcagg taacgcgcag
      661 tgagtggcaa gcgccgcagc tctttgacag tcgcgcgaag ctctgagaat cctgccgaga
      721 tgtaggtctt gcccggcggc aggttctttt tcgcgggccg tgatttcaat agcgtgaacg
      781 tgagaattga aaatgcgccc caccaaacac ccgcggacaa aagcgacaag cgaactgcca
      841 gacccgtcga aatacccaga cgctcggcct gcaacaccag aaacagattg aaaacgagca
      901 agatcgcacc gccaagatag ccgtatgcat agccgcgact cgacactttg tcggcttgat
      961 cgtcggtcgt gatctcaggc agaaacgcgt tgtaaaaaac gatcgacgcg ccaaaacaga
     1021 tgttcgccat gatgaaaaga acgccacccg ccaggtagag attgccctga acaaaaaaca
     1081 gcaaacagtt agcggccacg cctagatagc aaaacagcgc catcagtcgc ttcttcaggt
     1141 tcgagtagtc accgagtgcg ccgagaatcg gcagcaggaa gacctgcaaa cccaccgaga
     1201 tcgagacaca caacgttggc agagacttcg ctgtgacttc accgaatgga ccaaggtcca
     1261 tgacgacgcc gttctcgccg acagccgcct gggccagccg cgtgagatat ggtccaaaga
     1321 gcgccccgac gacagtcgta tagaacgcgg agttggccca gtcgtacatc ttccagccaa
     1381 agatctcacg cttgtcgttt ttatcggtca ttcttgtccg cagattacac agattacgca
     1441 gactcggtta ctgttctgtg taatctgtgg atacgtcctg aagaagccca cgctcgcgat
     1501 aaagattaag cttgttatgg agtgtcttga ggctgatgcc taacaattca gccgcgcggg
     1561 ttttgttgtt cttggtcttc tgaagcgtgc gcatgatcat ctggcgctcg acttcttcga
     1621 ggggcgtgcc aaccggcaac gagagcatat cttcactgcg catacgctcg cgttgatcga
     1681 tcgggtaagg tgcgaaatag tgacgttcga tttgttcgcc cgagcagatg atcactgcgc
     1741 gctcgatcac gttgcgaagc tcgcgcacgt tgccgggcca ggagtgaaac tgcaacgcgt
     1801 ccaacgctgc gggactcaga tagcgtgcgg gccggccgtg cttttctgcg agctgggtaa
     1861 cgagatgctg cgcgagtatt ggcagatctt cttttcgctc gcgcagtgac ggcaggtgaa
     1921 tgccgaaaac gttgagacga tagagcaggt cgctgcgtag tttgccttcc gaaactgcct
     1981 gttgcggcgg gcgattcgtg gcggcaagca agcgcacgtc gacttcgatc tctttacgac
     2041 tgccgagacg tcgcaccgct cgctcttcga tcacgcgcag gagcttggcc tgtaacaacg
     2101 ccggcatctc gccgatctca tcgaggagga gagtgccgga gtcagcgatc tcgaagcagc
     2161 cctgtcgtcg cgaggcggcg ccggtgaagg cgcctttctc gtgtccgaac agttctgatt
     2221 ccatcagcgt ttcggggatg gctgaacagt tgatcgcgac gaatggcttt tcgcgacgcg
     2281 ggctcaactg gtgaatggtg cgggcgacga gctctttgcc ggtgcctgat tcgccggtga
     2341 tcagaacaga agctgaagaa ggcgcgactt gttcgatgag tgtgtagatc ttgcgaattg
     2401 aatccgagct accgacgagt tcaccgtagg cgatgcgttc cgtagacatc cgcgccagtg
     2461 tagcacaggt attatccgcg tttatcagtg taaatccgcg gctgaaaaat aggccgcaat
     2521 tgcctgggcc agatctttgg tggtgaattg atccggttga atatcgattc gcagcccgta
     2581 ctcggcggca gcttcggcgg tgacggagcc gatgcatgcg acgaccatgc ccggcagtgt
     2641 tttgccgaga tcgtgagtgt cgaagagctt tgcgaggttt ttgatcgtgg aaggactagt
     2701 gaaggcgatg cagtcgccgc tgcctgcgag catggcggcg agtttcccgc gatcgatgtt
     2761 gtcaggaaga actgtctgat acgtggttac gacgtctaca cgcgcgcctg ctttttgcag
     2821 tgcttcaggc aataactcgc gtccgactac ggctcgtggt aagagaatgt tcaggccgcg
     2881 aagatgctca tcgccaccta cgaactcact caacgctgta aagacgcctt ccgcggttga
     2941 ttgtgatggg acgagatcga catgaacgtg ggcttcgtgg agtttgtctg cggttcgttg
     3001 gccgatcgcg cagactttga tctcgtcgag atctgagact tgatgcccgc gatcgatcag
     3061 gcgcttgagg aagaactcta cgccgttggt gctcgtgaag atgagccagt cgtagccgta
     3121 gagatggtcg agcgcttcgt cgagtcgatc gtagttgtcg ggctcgcgaa tctcgatcgt
     3181 cgggcaaacg atgactttgg cgccgtagct ttcgagtatc gtggtcagct ccgcagcctg
     3241 cgacgccgcg cgcgtgacga taacagttcg cccttcaagt ggtatccgat tcatcctgca
     3301 aatcctgtaa tcgtgtctca gttggggaca agattacacg attgacagga ttaacaagat
     3361 ccctacaaca aagcgcatgc gccacggtct atgagatgca cggcgagtga ggaaccaagc
     3421 ccttcgggat cctgaggtga tcccgatgaa gcaccgcgga ggatctcaga accgtcgggt
     3481 ttggcgacga gaccgtcgag tttcagcgaa tcaccgtcaa ggatggcgtg agcggcgatc
     3541 gggaattggc agccgccacc taatccacgc aaaaatgcac gctcggcgag acacgcgaca
     3601 cgagtttcgc gatggtccaa gcgagaagta ttttgaactg cgaactcgtt atccatgcgc
     3661 gtctcgatcg cgattgcccc ttgtccgacc gccggtaaca tctcaccgat cgcgatcctc
     3721 gcactgattc gctcttgcaa tccgagtcta acgagacccg cagacgcgag tatgaccgcg
     3781 tcgtactgtc cctcgtcgag ctttcgtatt cgcgtatcaa cgttcccacg cagatcttta
     3841 actaccacat cgcttcgcaa cgctttcagt tgcgcaagtc gtcgctggct tgatgtacct
     3901 acgactgctc cttcaggcaa attcagcaac gagccgctct cagatccggc gcgtaacacc
     3961 aacgcgtcgc gtgcatcttc gcgttcgcag atcgcagaaa tcgaaagccc ctccggcaag
     4021 atcgtcggca gatcttttaa cgaatgaacc gcgagatcta tgcggccatc gagcagcgcg
     4081 tcttcgagtt ctttcgtgaa cacgcccttg ccaccgatca ccgagagcgg atcgttcttc
     4141 acgtcgcccg tggttttgac gatctcgatg tgaacgtcaa gctgaggatt gagagcttgc
     4201 aaacgcgcgc gcgcctgttc agcctgccac agagcgagtt tcgagccgcg cgagccgatg
     4261 atcagagggc gagacatcgt cgatcagtcc tcgggaagat cattggaatc aggctcgccc
     4321 gtctcgtaaa aacgacgcat ctggctgatg attgg
//
LOCUS       DASSFQ010000164         5715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_973139, whole genome shotgun sequence.
ACCESSION   DASSFQ010000164 DASSFQ010000000
VERSION     DASSFQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973139"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFH96_05615"
     CDS             complement(<1..261)
                     /locus_tag="VFH96_05615"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6973481.1"
                     /translation="MSESVKLVVNGIEVEMPVGSMVSTAILKTGTHAFRRSVTGEPRG
                     PLCGMGICFECRVTIDGEQHCRSCQTVCINGMDVRTEPQKSTE"
     gene            complement(254..1375)
                     /locus_tag="VFH96_05620"
     CDS             complement(254..1375)
                     /locus_tag="VFH96_05620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6973482.1"
                     /translation="MSSKTYDVVIIGAGIVGSACAAECANEGLTVAIVEAGIIGGGAT
                     AAGMGHLTVMDDSEAQFALTRYSQQLWDQMSDALPSEVEHDACGTIWVAVDDEEMAEV
                     RRKEKFFAARGVKTEILDAQSLAEAEPNLRPGLVGGLRVPGDSVIYPPCAAQFFVDQA
                     RGKGAGLFLGIAVDKIEKGGVRLRDGSAISAGLVVNSAGSSSPALTRGLEVKKRKGHL
                     VITDRYPNFVRHQLVELGYLKSAHSMTAESVAFNVQPRKTGQLLIGSSRQFGVDDSHV
                     ENAIVTRMLERAVEYLPGLGKLSSLRAWTGFRAATPDKLPLIGPHTEHDRLYLATGHE
                     GLGITTSLATAKLLVDQIMNRDSAIPVAPYLPTRGAVHV"
     gene            complement(1372..2310)
                     /locus_tag="VFH96_05625"
     CDS             complement(1372..2310)
                     /locus_tag="VFH96_05625"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HET6973483.1"
                     /translation="MRRVTVIDSHTGGEPTRVVISGGPELGNDSLANRRERFKNDHDD
                     FRRAIVNEPRGSDAVIGALLCAPVDQSCVTGIIFFDRAGYLGMCGHGTIGVVATLAHL
                     NRIGPGLHRIETPVGLVVAHLDENKTVEVANVPSYRLAANISIEVPGVGPVRGDVAWG
                     GNWFFLVNEHSQELEFDNIDRLLDFTLRVREALARAGITGSDGHEIDHVELFGPSQLP
                     GVDSKNFVLCPSKTYDRSPCGTGTSAKLACLFADGKLKEGDVWKQESIVGSVFEGSIK
                     VVDDVVHPRIKGSAFITAEADLILDAHDPFAMGIGG"
     gene            2578..>5715
                     /locus_tag="VFH96_05630"
     CDS             2578..>5715
                     /locus_tag="VFH96_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973484.1"
                     /translation="MASAQAQSTPAQAREAEWKSYSLPQTNFAREIDPEKQFLFRVPA
                     DWKQQGDGLVFNGPYTSFIRVVVQKIPDGYPLQDYFAAVLKGVKDMPGAAEATLTRKT
                     QLQDLEAREIFLEMDNTEGETIRSTSWISVSGPLAVTFNFQAPLAHATELEPFFKAVV
                     QSVVFVSHDYQAFETLRSSLIKSSTPGPINEIESLVATLGEATSDRESAINRLASLFA
                     THGDVSVDLLLDRRPLIRAAAVQALARTSNTTLKPFLWKMIDDRELIVAEAAARSVAT
                     EPDVVAQTLKHSRSGFDTETIARIWPFMPSDKRVELLQQLFSKTATPPKTTPTVISGS
                     PKFDVSVKVGELKVVKPGTPAPDVTVGVSNETDVQLGALMLLSTVGRDEFKLPLARLT
                     ASDYDPLIAVGLQVAEQRGEALPVESLLKLVASSDQPVSRLAVQSLGLSASVADIPRI
                     EALVSKNSPAAATKSLDDELKLSIKKIRFRNELSTAKTLAEQRQIIGKALTDPLLDEF
                     AWRYDCEATVDGCGPAPAAPKTDFIVKPFGENLFPRKVRHYTAIPAPGQSVQRFYETL
                     NGLQLETPRAQAGLVLMIGYLRQLLANQLSAPAEAATLIEYTGIDPNSPIAMGTWTAD
                     KALDTTSFAERRAIVLRVKDRARFERAIQALQSSDTGVTNFIDYVAVATRAAAALPAV
                     LPLGVASLRANDPKKPSKHTLLSYSFTTEKEWNGLRVKTIEHRSVNRDMQILGAATHI
                     VFIGDTVILASDLAAIRDLLVRTGSDENLAANAEFRKAIERRGDVVYFSDLKSVLGAS
                     GIDTKDSGSNINESGAFNISNSSWENSHQLVFDESEWSRPLQPFHPKELTAPRELLPA
                     STIAYYLMKVDLASAWPTELRKYFLPDDRQDKSNVWALDLKEVLAELGPECGAVVLEL
                     PYLKDFDELTWAGFCKLKSTKLSEALIAGKLFTGVGPTKDFAEVKVGNDSYFVATRKG
                     FLVVSNHDKGLAVFDGKSNLAETRDYSRAVDKVPTGIVAFGGYNLEAAIAAANKIPFE
                     GTQAFAANLIGSVASA"
BASE COUNT         1278 a         1677 c         1536 g         1224 t
ORIGIN      
        1 ttctgtgctt ttttgtggct ctgttcgcac gtccatcccg tttatgcaaa ccgtctggca
       61 actacgacaa tgttgctcgc cgtcgatcgt cacacggcac tcaaaacaga tccccatgcc
      121 acacaatgga ccacgcggct cacctgtcac cgaccgtcga aacgcatgtg tgcccgtttt
      181 cagaatagcc gtagacacca tgctcccgac gggcatttcg acttcaattc cattgacgac
      241 gagcttcaca gactcagaca tggaccgcac ctcgggtagg caggtaaggc gcgaccggaa
      301 tcgcgctatc gcggttcata atttgatcga cgagcaattt cgcagtggcg agtgaggtcg
      361 tgatgccgag gccttcgtgg ccggtcgcga gataaagccg gtcgtgttcc gtgtgtggtc
      421 caatcagcgg tagtttgtca ggcgttgccg cgcgaaatcc ggtccatgca cgtaacgacg
      481 agagcttacc gagacccggc aaatactcaa ccgcgcgctc gagcatgcgg gtgacgattg
      541 cattctcaac gtgcgagtca tcaacgccga actgtcgtga cgagccgatc aataattgac
      601 cagtcttgcg tggttgaaca ttgaacgcga ccgattcggc cgtcatcgag tgcgcactct
      661 tcagataacc aagctcgacc agttgatgcc gcacaaaatt cggatagcga tcggtgatca
      721 cgagatggcc tttgcgcttc ttcacctcga gcccgcgtgt caacgccggc gacgaactac
      781 cggctgaatt gacgaccagt ccagcagaaa tcgcactgcc gtcgcgcaac cgaaccccgc
      841 ctttttcgat tttgtccacc gcaataccga gaaaaagccc ggcgcctttc cctctcgcct
      901 ggtcgacaaa aaattgcgcc gcacacggcg gatagatcac gctgtcgccc ggaacacgaa
      961 gcccaccgac caatccgggc cgcaaatttg gctcagcttc tgcaagcgac tgcgcatcga
     1021 gaatctcggt cttcaccccg cgcgcagcaa agaatttctc tttgcggcgt acttccgcca
     1081 tctcttcatc atcaaccgcg acccagatcg tgccacacgc atcgtgctcg acttcgcttg
     1141 gcaacgcgtc gctcatctga tcccagagct gttgcgagta acgcgtgagg gcaaactgag
     1201 cttccgaatc gtccatcacc gtgagatggc ccatgcccgc ggctgtcgca ccgccgccga
     1261 tgattcccgc ctcgacaatc gcgactgtca gaccctcatt cgcacactcc gccgcacacg
     1321 cggatccgac gatacccgcg ccgatgatga cgacgtcgta agttttcgaa ctcatccgcc
     1381 aatgcccatg gcaaagggat cgtgcgcatc gagaatcaga tctgcttcag cggtgatgaa
     1441 agccgagcct ttgatacgcg ggtggacaac atcgtcgacg actttgattg aaccttcaaa
     1501 gacgctgccg acaatactct cctgcttcca cacgtcgcct tcttttagtt tcccatcggc
     1561 aaacagacac gcgagttttg cgctcgtgcc tgtgccgcaa ggcgagcggt cgtaggtttt
     1621 actgggacag agaacgaagt ttttactgtc aactccgggt agttgcgatg gaccaaaaag
     1681 ctctacgtga tcgatctcat gcccatcgga acccgtgatt ccggcccgag caagcgcttc
     1741 gcgaactctc agagtaaaat ccagcaagcg atcgatattg tcgaactcca actcctggct
     1801 atgctcgttc acgagaaaaa accagtttcc gccccacgca acatctcctc taactgggcc
     1861 aacaccggga acttcgatcg agatattggc ggcgagacga tagctcggaa cattcgcgac
     1921 ttccacagtt ttattctcat cgagatgcgc gacgacgagg ccgaccggcg tctcgatccg
     1981 gtgtaacccg ggaccgatgc ggttcagatg ggccaacgtc gcgactacgc cgatcgtgcc
     2041 atgcccgcac atgcccaaat aaccggcgcg atcgaaaaag ataatgccgg tgacgcacga
     2101 ttgatcgact ggagcacaca gcaaagcgcc gatgaccgcg tcagagccac gcggctcgtt
     2161 cacgatcgcg cggcgaaagt cgtcgtgatc gtttttgaac cgctcgcgac gatttgcgag
     2221 agaatcgttg ccgagctctg gcccaccgct gatgaccacc cgcgtgggct caccgccggt
     2281 gtgtgaatcg ataactgtga cgcgtcgcat gccgcatatt gagccacaaa aaggcacaaa
     2341 agtcacaaag caagataaac ttatctctgc ggccctctgc gtatctttgc gttctctgcg
     2401 ttaaagggct atttcaacgc agaggccgca gagatacgca gagggccgca gagaaatctc
     2461 tttctggttt gtgccttttg cgcctttttg tggcagaatt cacgatttcc gaaaggcaag
     2521 tagaccatgc gcaagctgtt ccccctcttc ctccttcttc ttctgttgtg caccagtgtg
     2581 gcttccgccc aggcgcaatc gacgccggcc caggcccgtg aggctgaatg gaagagctac
     2641 tcactcccgc agacgaattt cgcgcgggag atagatcccg agaaacagtt tctctttcgc
     2701 gtgcccgccg actggaagca acagggagac gggcttgtct tcaatggtcc atacacgtcg
     2761 ttcataagag ttgttgtcca gaaaataccc gatgggtacc cgttgcagga ttattttgca
     2821 gcggttctca agggtgtgaa agacatgccg ggtgcggccg aggcgacgtt gacgcgcaaa
     2881 acgcagctcc aggatctgga agctcgcgag atctttctcg agatggataa cactgaaggt
     2941 gagaccatcc gcagcacttc gtggatctct gtcagtggac cactcgcagt gacgttcaat
     3001 tttcaggcgc cgctcgcgca tgccaccgag ctcgagccgt tcttcaaggc cgtggtgcaa
     3061 tcagtggttt ttgtttccca cgactatcaa gccttcgaaa cgctgcgctc ttcgctaatc
     3121 aagtcgtcca cgcctggtcc aatcaacgag atcgagagcc tcgtcgccac tctcggtgaa
     3181 gctacttccg atcgagaatc ggcgatcaac cgtttggctt cgctctttgc cacgcacggt
     3241 gacgttagcg tagacctgct gctcgatcgt cgtccgttga ttcgcgccgc cgccgttcag
     3301 gcactcgcac gcacaagcaa caccacgctc aaaccgtttc tgtggaagat gatcgacgac
     3361 cgggagctaa tcgttgcgga agcggcggcg cgcagtgtcg caaccgagcc ggacgttgtg
     3421 gcccaaactc taaaacactc aaggtcaggc ttcgacacag aaacgattgc tcgaatctgg
     3481 ccgttcatgc cgagcgacaa gcgagttgag ctgctgcaac agcttttcag caagaccgct
     3541 acgccaccaa aaacaacccc tacggttata agtggttctc cgaaattcga tgtttcggta
     3601 aaagttggcg agctcaaggt tgtgaaaccc ggaacgcctg cgcctgacgt gacggttggc
     3661 gtttccaacg aaaccgatgt tcagctcggc gcgctaatgc tgctcagcac ggtggggcgt
     3721 gacgagttca aacttccact cgcgcggctc acggcgtcag attacgaccc gctgatcgca
     3781 gtcggcttgc aagtggcgga acaacgtggt gaagcactgc cagtcgagtc actactcaaa
     3841 ctcgtagcct cttccgatca gccggtgagt agactcgcgg tccaaagtct cgggctttcc
     3901 gcaagtgttg ctgacattcc tcgcatcgag gcgctcgttt cgaaaaactc ccccgccgct
     3961 gcgacgaagt ctctcgatga tgagctcaag ctttcgatca aaaagattcg atttcgaaat
     4021 gagctgagca ctgcaaaaac tctagccgaa cagcgtcaga tcattggcaa agctctgact
     4081 gatccgttgc tcgacgagtt tgcctggaga tacgactgtg aagcgacagt cgatggctgt
     4141 gggccagcgc cagccgctcc caaaacggat ttcatagtta agccgtttgg tgaaaatctg
     4201 tttcctcgca aagttcggca ctacactgcg attccggctc cgggccagtc tgtgcagcgg
     4261 ttctacgaaa ccttgaacgg acttcagctc gaaactccgc gcgcgcaggc gggtctcgta
     4321 ttaatgatcg gctacctgcg tcagttactc gcgaaccagt tgagcgcgcc cgctgaagcc
     4381 gccacgctga tcgaatacac cgggatcgat ccgaattcgc cgatcgcgat gggcacatgg
     4441 actgccgaca aagcgctcga caccacgagc tttgcagaac gaagagcaat cgtgttgcga
     4501 gtcaaagatc gggcgcggtt tgagcgagcc atacaggcgc ttcaatcatc tgacaccggc
     4561 gtcactaact tcatcgacta cgttgcagtc gcgacgcgtg ctgctgctgc tctgccggcc
     4621 gtattgccgc tcggtgtcgc gtcgctgcga gcaaacgacc cgaagaaacc gtcaaaacac
     4681 accctcctct cgtactcgtt tactactgaa aaggagtgga acgggctgcg cgttaagacc
     4741 atcgagcatc gctccgtcaa ccgcgatatg cagattttgg gcgctgccac tcacatcgtc
     4801 ttcattggcg acactgtgat tctcgcgtca gatctcgcgg cgattcgaga cctgctggtc
     4861 cgtaccggca gtgacgagaa cctcgccgcc aacgcggagt ttcgcaaagc gattgagcgt
     4921 cgtggcgacg tcgtttattt cagcgatctg aaaagcgtgc tgggcgcgtc gggaatagac
     4981 accaaagatt ccggctccaa catcaacgag agcggggcct tcaatatctc aaacagctcg
     5041 tgggaaaact ctcaccagct cgtgttcgat gagagtgaat ggtccaggcc tttgcagccg
     5101 tttcatccaa aggagctgac ggcgccgcgc gagttgttgc ctgcttcgac gattgcttac
     5161 tacctgatga aagtcgatct ggcgagtgcg tggcccacgg agttgagaaa gtatttccta
     5221 cctgacgaca gacaggataa gtcaaatgtg tgggcgctcg atttgaaaga ggtgctggct
     5281 gagctcgggc cggaatgtgg cgctgtcgtg ctcgagcttc cctacctcaa agacttcgac
     5341 gaattgacgt gggccggctt ctgcaagttg aagagcacga aactttctga agcgctgatt
     5401 gccggtaaac ttttcactgg cgttggtcca acgaaagatt tcgcggaagt taaggtcggg
     5461 aatgacagct attttgtcgc gacgcgcaaa gggtttctcg tggtttcgaa tcacgataag
     5521 ggactcgcgg tgtttgacgg caagagcaac ctggctgaaa cgcgtgacta ctcgcgcgct
     5581 gtggataaag ttccgaccgg cattgtcgct ttcggcggct acaacctcga agccgcaatc
     5641 gctgcggcaa acaagattcc gtttgaggga acacaagctt tcgcagccaa cctcatcggt
     5701 tccgtcgcga gcgcg
//
LOCUS       DASSFQ010000165         9377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1109318, whole genome shotgun sequence.
ACCESSION   DASSFQ010000165 DASSFQ010000000
VERSION     DASSFQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109318"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VFH96_05635"
     CDS             complement(<1..949)
                     /locus_tag="VFH96_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6973485.1"
                     /translation="MADGDLSFKIGPDQAGIRLDTYLASQIEGWSRARLQRLIENEDV
                     LVNGKIAKPSYKLRDGDEIEVELVAAPTDVFTPENIPLDIVYEDDVLVVVNKPAGLVV
                     HPSAGTPSGTLANALAYHFQKLPGTGVRPGIVHRLDRDTSGLLVVAKTDAALENLSDQ
                     FRDRTVYKSYVALVHGRVPSNSGKIDQPLARDPSNRTRMAVVRAGRNALTLFRVRRSF
                     ERFTLLDVELKTGRTHQIRVHLAWMKYPVVGDETYGGGRDNTIRDPRLRARIRALHRH
                     FLHAEKLGFKHPATGEFVKFEAPLPDELLELLTALERPAG"
     gene            complement(942..2027)
                     /gene="serC"
                     /locus_tag="VFH96_05640"
     CDS             complement(942..2027)
                     /gene="serC"
                     /locus_tag="VFH96_05640"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET6973486.1"
                     /translation="MTERIFNFSAGPAVLPVPVLEEAQRDMLSLPGVGMSVMEISHRS
                     KTFDEIINRTEANLRDLLKISSNYHILFLQGGASLQFSMVPMNFLPADGSADYILTGS
                     WGKKALKEAKKVGGVNLAATMADGGFTRVPSQDEISLNPHAAYVHITSNETIEGVQWK
                     REPNVGDVPLIVDSSSDILSRELDVDKYALIYAGAQKNMGPSGVTLVIVRDDLLQRIP
                     ENLATMLDYGIHVQNKSLYNTPNTWGIYIMDLVCKWLQDQGGLAGMEKQNDEKAQLLY
                     DAIDATDFYRGHADVDCRSPMNVTFRLPSEELEKKFASEATAQGLDGLKGHRSVGGIR
                     ASIYNAFPRAGVEALVSFMKDFERRNG"
     gene            complement(2078..2854)
                     /locus_tag="VFH96_05645"
     CDS             complement(2078..2854)
                     /locus_tag="VFH96_05645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6973487.1"
                     /translation="MNTTLITGASTGIGAAFARKLAARGRNVLLVARSEDKLIALCNE
                     LGRLTSIRAQYLALDLQQPDAGVQLFEETKKRELEIEMLINNAGFGSMGDFKKLDLDR
                     ELEIIQLNVRALVDLTHRFLGPMRERKGGTIINVASTAAFQAVPYMATYAATKAFVLS
                     FSEAIAEENRLHGIHVMALCPGVTETNFFEAAKIDRPPMRTIQTAEQVVDAALRGLDR
                     HKTTVISGWTNWIMAEAERFVPRSTVTKIAGKALRSRFEE"
     gene            complement(2932..4137)
                     /locus_tag="VFH96_05650"
     CDS             complement(2932..4137)
                     /locus_tag="VFH96_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HET6973488.1"
                     /translation="MRVLIADKFEQSGRDGLESLGCEISFQPDLKDDALVEAIRKEAP
                     DVLVVRGTKVTEPMFDAGPVKLVVRAGAGYNTIDVAAASKRGIYVSNCPGKNSIAVAE
                     LAFALILAVDRRIADNVVTLRRGEWNKKEFSKARGLFGRTLGLVGVGKIGQEMIPRAR
                     AFGMPVVAWSRSLTAERAAELGVELKSSPREVASASDIVSVHVALTPETKHFLSTDFF
                     NAMREGSYFINTARGEVVDQQALVAAMHNRGIRTGTDVYAAEPTSATGEIHDEIVKEE
                     GLYGTHHIGASTDQAQEAIAAETVRIVKEFKETGKVPNVVNLARQTPATHRLVVRHRD
                     RPGVLAGVLDAIKAEHINVQEMENIVFEGAAAAVARINLDNAPTKDTLSKLQASNADI
                     IELDLLELL"
     gene            complement(4140..5366)
                     /locus_tag="VFH96_05655"
     CDS             complement(4140..5366)
                     /locus_tag="VFH96_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HET6973489.1"
                     /translation="MAIIRPFRALRPQPERAQEVASVPYDVVNTEEARALAEGKPLSF
                     LHVSRPEIDLPPDTDIHSDLVYRKAVENFEKLIAKAPLEKETEPSLYLYRLIMGDHEQ
                     IGVVACASIDEYDDGTIRKHERTRRDKEDDRTRHMLMLKAQTGPVFLTYRARPEIDEQ
                     ISAAINAESLFDLTAPDGVRHTIWRLRETESLVKSFESVPLLYIADGHHRAASASRAR
                     AELKQQSFAHTGEEDYNYFLTVIFPDSQMQILAYNRTVHDLNGLSPQEFLNAIQEQFT
                     ISENANPEPAQRGHWSMYLGGKWYGLQQSPAATLPTGTVAMLDVSILQDRLLDPILGI
                     KDIRTDKRVDFIGGLRGTKELERLVNEGKAAVAFSMFPTTVEELLMVSDANEIMPPKS
                     TWFEPKLRDGLLIHTI"
     gene            5462..5911
                     /locus_tag="VFH96_05660"
     CDS             5462..5911
                     /locus_tag="VFH96_05660"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HET6973490.1"
                     /translation="MNKIAKTIRLLSRGARKIVTRPGEAWLMLRMAWWVAVLSAMARC
                     YSLPRALQIVAGNQKRSGEALNREELARTIDLLLSADVLVFRPICWKRAAVLHRYLSR
                     NGTKTKIIFGVRNESGGKFDGHAWLEVDGQPILEKDPPNYVVTYSFP"
     gene            complement(6000..7511)
                     /locus_tag="VFH96_05665"
     CDS             complement(6000..7511)
                     /locus_tag="VFH96_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973491.1"
                     /translation="MSKSLRGSAVFAGLVVMVLGVSQVAFAGGATTQQSQDPAASSSE
                     TSKVAKSQTKSKKSKKKKATNNSFIVLGTYAPQTTVLPSASSNITPMAAEPLVTPRRV
                     VAAEKTPGIHILPTEEPPATVASAGQLVISEFRVRGINGLNDEFIEIANVSGADHTVA
                     PLSGTGYGIAASDGTTRCSIPTGTVIPNRGHYLCVNSIGYSLASYPAGNGTTATGDAT
                     YTTDIADNAGIAIFNNNTGGASYSLPNRLDAVGSTSEANTLYKEGTGYPALVPFSINY
                     SFYRDNCGKGGSITTFGACPTGGNLKDTNNNAADFVFVDTNGTSAGAGQRLGAPGPEN
                     LSSPIERNASFAVNLLDVCTAPASPPNRVRDFTSDPPNNSTFGTLDVRRTVVNNTGGN
                     VTRLRFRVVDLTTFPAPSGIADLRPRSSNAVVVTVDRPPCGSGTSNVTVQGTALEQPP
                     SQANGGGFGSSMSVGVVTLATPIANGASVDVRFLLGIQQTGSFKFFINVEALP"
     gene            complement(7653..8528)
                     /locus_tag="VFH96_05670"
     CDS             complement(7653..8528)
                     /locus_tag="VFH96_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET6973492.1"
                     /translation="MAQGIVNRVVPSSPYALGIVRFFVHGIFLFDTVVTSYSALGSLP
                     PTILRPTGVMKFFPWRVYDALQTQGGMLTLKTLMLLSLALSTAGLFTIISTKTSLLLV
                     LFYQGLLRSFGHFNHDEMIAVYFLVVLAFTPCGDAFSIDSRFAKTKSQPPAFAYAYPI
                     LLMQLLLAWVYFSSALIKLRVAGWKYLSTDNLPALAIFHSLDNLHDTAFRGAFWLPQV
                     REYLPYAVGFILVWELLFPLAIFFRRLRWLILAIGFVFHVATVPLMNIGFPHQLLMYV
                     VFVNWDRRKLVKSGN"
     gene            complement(8510..9076)
                     /locus_tag="VFH96_05675"
     CDS             complement(8510..9076)
                     /locus_tag="VFH96_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973493.1"
                     /translation="MSIISKLTNSWAQADDDAKRLVIKYAILTAALFLPLFWAFVAVR
                     NLYPITAWNVMMAGGDLERGRSYCILRGETVSGETIDVAAIQLTNALYSRNWTMVNAT
                     IDNQAFKLKSIHPQNEEMLRQFGGIENLPPGARISDLLQAWGNIYNKREPESSPKRLK
                     AIRLDMYRWEGGHYADYDKFIESWRKEL"
     gene            complement(9033..9299)
                     /locus_tag="VFH96_05680"
     CDS             complement(9033..9299)
                     /locus_tag="VFH96_05680"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HET6973494.1"
                     /translation="MNELRPGKHVVATDFDGGEGILVDLNTKKYYQLNETAMVVWKGL
                     EKGKSMGEIVADITATYEVPADHATSSVQRIVDNFQTYKLLGAS"
BASE COUNT         2066 a         2599 c         2579 g         2133 t
ORIGIN      
        1 cgcccgctgg tcgttctaac gcagtcaaca attcgagtag ttcatcaggt aaaggtgcct
       61 cgaattttac aaattccccg gtcgccggat gtttgaatcc gagtttctct gcgtgcagga
      121 aatgccggtg aagagcgcgg atacgggcgc ggaggcgcgg atcccggatc gtgttgtcgc
      181 gtccaccgcc atacgtttca tcaccaacca ccggatactt catccacgcg agatgcacgc
      241 ggatttgatg cgttcttccg gttttcaact ctacatccaa caacgtgaat cgttcgaacg
      301 atctccgcac gcgaaacagc gtcaacgcgt tacgcccggc acgcacgacc gccatgcgcg
      361 tgcggttcga aggatcgcgg gcgagcggct ggtcaatctt tcccgaattt gaaggcacac
      421 gcccgtgcac caacgccaca tacgacttgt aaacagttcg atcgcgaaac tgatccgata
      481 ggttctcgag cgctgcatca gtcttcgcaa ccacgagcag ccctgaagta tcccgatcga
      541 gccggtgaac aattccgggt ctcaccccgg ttcccggcag cttttgaaaa tggtacgcga
      601 gcgcgttcgc aagcgtgcct gaaggcgtgc ctgctgaggg atggaccacg agaccggccg
      661 gtttgtttac aaccaccagc acgtcgtctt cgtaaacgat gtcgagcgga atattttcag
      721 gagtaaagac atcggtcgga gctgcaacga gctcaacttc aatctcgtct ccgtcgcgga
      781 gcttgtacga aggcttagca atcttgccgt ttacgagtac gtcttcattc tcaatgagac
      841 gctgcaaacg cgcgcgactc cagccctcga tctgtgaggc gagatacgtg tctaaacgaa
      901 tgccagcctg gtctggtcca atcttgaaac tgagatcgcc atcagccatt gcgacgttcg
      961 aaatctttca tgaacgacac gagcgcttcg acaccggcac gcggaaacgc gttatagatc
     1021 gacgcgcgaa tgccaccaac agatcgatgc cctttcaagc cgtcgagtcc ctgcgctgtt
     1081 gcttcagaag caaacttttt ctcgagctct tccgaaggca gtcggaacgt gacattcatc
     1141 ggcgagcgac aatcgacatc agcatgaccg cgataaaaat ccgtcgcgtc gatcgcatcg
     1201 taaagcaact gcgccttctc gtcgttctgc ttctccattc ccgcgagacc gccctggtcc
     1261 tgtagccact tgcaaacgag gtccatgatg taaatgcccc acgtgtttgg cgtgttgtag
     1321 agcgatttat tttgcacgtg aatgccatag tcaagcatcg tcgccaaatt ctcgggaatg
     1381 cgctgcaaca gatcatcgcg aacgatcacg agcgtgacgc cgctcggtcc catgttcttc
     1441 tgcgctccgg cataaatgag cgcatactta tcgacgtcga gctcgcgcga aagaatatcg
     1501 gacgaggaat cgacgatgag tggaacgtcg ccgacattcg gctcgcgttt ccactgcacg
     1561 ccttcgatcg tttcattgct cgtgatgtga acgtacgcgg cgtgcgggtt caaactgatc
     1621 tcatcctgtg acggcacgcg agtgaagcca ccgtcagcca tcgtcgcagc aagattcacg
     1681 ccgccgactt tttttgcttc cttgagcgcc ttcttgcccc agcttccggt gaggatatag
     1741 tcggcgctgc cgtcagcagg caggaagttc atcggcacca ttgaaaactg caaactcgcg
     1801 ccgccttgta gaaaaagaat gtgatagttc gacgaaattt ttaagaggtc gcgcaggttc
     1861 gcttcggtgc gattgatgat ctcgtcgaat gttttcgaac ggtgactgat ttccatcact
     1921 gacatgccga cgcccggcag gctcagcatg tcgcgttgtg cttcttctaa aaccggcacc
     1981 ggcaacaccg ccggccccgc gctgaagtta aagattcgtt ccgtcattga tgtctccagt
     2041 aataggacct atgtaggacg aataggacct atttctttta ctcctcaaat ctcgatcgca
     2101 aagcctttcc cgcgatcttc gtcaccgtcg agcgtggcac gaagcgttcg gcttcagcca
     2161 taatccagtt ggtccaaccg gaaatcactg tggtcttgtg ccggtcgagc ccacgcaacg
     2221 ctgcgtctac gacttgctcc gccgtctgaa tcgtacgcat cggcggtcga tcgatcttcg
     2281 ccgcttcaaa aaagtttgtc tcagtcacgc ccggacacag cgccatcacg tgaattccgt
     2341 gcaagcgatt ctcttccgca atcgcttccg aaaacgacag cacaaacgcc ttcgtcgctg
     2401 cgtacgtcgc catgtatgga acagcctgaa aagccgcggt cgaggcgacg ttgatgatag
     2461 tgccgccctt tcgttcgcgc atcgggccaa gaaaacgatg cgttagatcg accagcgctc
     2521 gcacgttcaa ctgaataatc tcgagctcgc gatcgagatc gagtttctta aagtcgccca
     2581 ttgagccgaa gccggcgttg ttgatgagca tctcgatctc gagctcgcgc ttcttcgtct
     2641 cttcaaacaa ctgcacgccg gcgtcgggtt gttgcagatc gagcgcgaga tactgtgcgc
     2701 gaatactcgt caaccggccg agctcgttac agagagcaat cagcttgtct tccgagcgcg
     2761 cgaccagcaa cacgtttctc ccgcgcgcgg cgagttttct ggcaaaggct gcgccgatcc
     2821 cggtcgatgc tccagtgatg agggttgtgt tcattatgag atagtttagc cacagattac
     2881 acggatgaca ctgattaaat ttcttatctg tgttatctgt gtcatctgtg gctaaagcaa
     2941 ctccagcaaa tcgagctcga ttatatctgc gttgcttgct tgaagcttgc tgagggtgtc
     3001 ttttgtcggc gcattgtcga ggttgatgcg ggccaccgcc gctgccgcac cttcaaacac
     3061 gatattttcc atttcctgaa cgttgatgtg ctcggccttg atcgcgtcaa gcacgcctgc
     3121 aagcacaccg ggccgatcgc gatgacggac aacaaggcgg tgcgtcgcgg gcgtttgccg
     3181 cgcgagattc acaacgttcg gcaccttccc ggtttcctta aactctttca ctatgcgaac
     3241 agtttctgct gcaatcgctt cctgcgcctg atccgtcgag gcaccgatgt gatgcgtgcc
     3301 gtaaagtcct tcttccttca cgatctcatc gtgtatctcc ccggtcgcgc tcgtcggctc
     3361 cgccgcgtac acatccgtgc cggtgcgtat gccgcggttg tgcatcgccg cgacgagtgc
     3421 ctgttgatcg acaacttcac cgcgcgcggt gttgatgaaa taactaccct cgcgcatcgc
     3481 gttgaaaaag tcagtactga gaaaatgctt cgtctcggga gtcagtgcga cgtgaacact
     3541 cacgatatca gacgcgctcg cgacctcgcg cggtgacgac ttcaactcaa cacccaactc
     3601 ggccgcgcgc tccgcagtca agctccggct ccacgcaacc acaggcatcc caaacgcccg
     3661 tgcgcgcgga atcatttcct gaccaatctt gccgacgccg accaaaccaa gcgtgcgtcc
     3721 aaacagtccg cgtgctttcg aaaactcttt tttgttccac tcaccgcgtc gcaaggtgac
     3781 gacgttatcg gcgatgcgtc gatcgactgc gaggatcagc gcaaacgcga gctcggccac
     3841 ggcgattgag tttttgccgg gacaattcga aacgtagata ccgcgcttcg aagccgcggc
     3901 gacatcgatc gtgttgtatc ccgcgccagc gcgcacgacc agcttcactg gcccggcatc
     3961 gaacatgggc tcggtgactt ttgtccctct caccacgagg acgtccggcg cttctttgcg
     4021 aatcgcctcc acgagcgcgt cgtccttcaa atcaggttga aaagagatct cgcaaccgag
     4081 cgactccaag ccgtcccggc ctgattgttc gaatttgtct gcaatgagaa ctcgcataat
     4141 taaatcgtgt gtatcaacaa tccgtccctg agtttcggct cgaaccacgt cgacttcggc
     4201 ggcatgattt cgtttgcatc agaaaccatc aacaactctt cgaccgtcgt cggaaacatt
     4261 gaaaacgcaa ctgcagcttt gccttcgttc acgagacgtt cgagctcttt cgtgccgcgc
     4321 aggccgccga tgaagtctac tcgtttgtca gtgcgaatat ctttgatgcc gagaatcgga
     4381 tcgagcaagc ggtcctgaag aatactcaca tcgagcatcg cgaccgtgcc cgttggcaaa
     4441 gtggctgcag gtgattgttg caaaccgtac cacttaccac cgagatacat gctccagtgg
     4501 ccgcgctgcg ccggctcggg attcgcgttc tcactaatcg tgaattgttc ctgtatcgcg
     4561 tttagaaact cttgcggcga aaggccgttg agatcgtgaa cagtgcggtt atacgcaagt
     4621 atctgcattt gcgaatccgg gaagatcacg gtcaggaaat agttgtaatc ctcctcgccg
     4681 gtatgcgcga agctttgttg tttcaactcg gcgcgcgcgc ggctcgcact cgcggcacgg
     4741 tgatgaccgt cagcgatata aagcagcggc actgattcaa aactcttcac gagcgattcg
     4801 gtttcacgca aacgccagat cgtgtgacga acgccgtccg gcgctgtcag atcaaacaac
     4861 gactccgcgt ttatagccgc tgagatttgt tcgtcgattt cgggtcgcgc tctgtaggtt
     4921 aggaaaaccg gtcccgtctg cgctttcaac atgagcatgt ggcgggtgcg atcgtcttct
     4981 ttgtcgcgac gcgttcgttc atgtttgcga atcgtgccat cgtcgtattc gtcgattgaa
     5041 gcgcaggcta cgacgccgat ctgctcgtga tcacccatga tcaggcgata taaatagagg
     5101 cttggctcag tctctttttc gagcggcgcc ttcgcgatca gtttttcaaa attctcgact
     5161 gcttttcgat agacgaggtc gctgtggatg tctgtgtccg gcggcaagtc gatttccggc
     5221 cgcgatacgt gcaggaaact gagcggcttt ccttcggcga gcgcacgcgc ctcctcagtg
     5281 ttaactacgt cgtacggcac gcttgccact tcctgggcgc gctcaggttg tggacgcaaa
     5341 gcacgaaagg gacgaatgat cgccacagat cctcctgatg atatgagtta ggctgatgtg
     5401 gtctaagact agccgtttag gtgcgaaaac tcaaactacg ctagaatcaa gcggccgaag
     5461 gatgaacaaa atcgcgaaaa ctattcgact cctttcacgt ggcgcgcgca agatcgtgac
     5521 gcggccgggc gaggcgtggt tgatgttgcg aatggcgtgg tgggtcgctg tgctttctgc
     5581 gatggcgcga tgttactcac tgccgcgtgc gctccagatt gtggccggta atcagaagcg
     5641 ttcaggcgaa gcgttgaatc gtgaagagct ggcccgcacg atcgatctac ttctatccgc
     5701 tgacgtactg gtgttcaggc cgatctgctg gaagcgggcc gccgttttgc atcgatatct
     5761 atcgcgcaac ggcactaaga cgaagatcat cttcggcgtg cggaatgaaa gcggcggcaa
     5821 gttcgatggc cacgcctggc tcgaggtaga cggccagccg atcctcgaaa aagaccctcc
     5881 gaattacgtt gtgacgtaca gttttcctta acgcggattc acgcggatag acgcggataa
     5941 aaaccaaaag ggagaggcgc gtgtgcacct ctcccttttt catgattaat tgttcagcgt
     6001 tacggcagag cttcgacgtt gatgaagaac ttaaagcttc ctgtttgctg aatgccaagc
     6061 aggaatcgca cgtcgacact cgctccattc gcgatcggcg ttgccagcgt tactactcca
     6121 accgacatcg acgaaccgaa tccgccgccg tttgcttgtg acggtggttg ttcgagcgcc
     6181 gttccctgaa cagtcacgtt gctggttcca ctgccgcacg gcgggcggtc aacagtaacc
     6241 acaacagcgt tggaagatcg aggacgcaag tcagcaatgc cgctcggcgc cgggaacgtc
     6301 gtcaggtcaa ccacacggaa acgcaagcgc gttacgtttc ctccagtgtt attcacgaca
     6361 gtcctccgga cgtccaacgt accgaacgta gagttgttgg gcggatccga agtgaagtcg
     6421 cgaacgcgat tcggcggtga tgccggagca gtacaaacgt cgagaaggtt caccgcaaaa
     6481 gatgcatttc gctcgattgg cgagctgagg ttctctggtc caggtgcgcc gagacgctga
     6541 cctgcacctg ctgacgtgcc gttggtatca acgaacacga aatcggcagc attgttgttt
     6601 gtgtctttga gattcccacc ggtggggcag gcgccgaacg tagtgatcga gccacccttg
     6661 ccgcagttat cacgatagaa cgagtaattg atcgagaacg gaaccagcgc cggatatccg
     6721 gtaccttcct tgtagagtgt attcgcttcg gaggtcgatc caacggcgtc gaggcgattg
     6781 ggcaacgaat aagaggcgcc gccggtgttg ttgttgaaga tagcaatgcc ggcattgtct
     6841 gcgatatcgg tggtataggt ggcatcgccg gtggcagtcg ttccattgcc tgcggggtag
     6901 gaggcgagac tgtagccaat ggagttgacg cagaggtaat gacctctatt cgggatgacc
     6961 gtgccggttg ggatcgagca tcgggtggta ccgtcagagg cagcaatgcc atagccagta
     7021 cctgagagcg gagcgacagt gtggtcggcg ccgctcacgt ttgcgatttc aataaactcg
     7081 tcgttcaggc cattgatgcc acgaacgcgg aactcggaaa taacgagttg acctgccgaa
     7141 gccacggtag cgggaggctc ttcagtgggc aaaatgtgaa taccgggagt tttctcggct
     7201 gctacgactc gtctgggagt gacaagcggc tcagcagcca tcggtgtgat attggaactt
     7261 gcgctcggca aaacagttgt ttgcggcgcg taggtgccca aaacgatgaa actattgtta
     7321 gtggcttttt tctttttgga tttctttgat ttggtttgag attttgcaac cttgctcgtc
     7381 tcggaactac tcgcggccgg gtcttggctt tgttgggtcg tggcgccgcc ggcgaaggcg
     7441 acttgcgaaa cgccgaggac catcacgacc aggccagcga agaccgcgga tccccgcaat
     7501 gacttagaca tgcatgtatt ctcctttaaa ggtgggccca cgatgttcag tgtggcgctc
     7561 gtcaccgtac catgcttgtt tcaacccagt caacgaaatt aaacattaca aatgtattgt
     7621 tatgtacgaa ttacgatagg agataggacc agttaattgc ctgatttgac caacttgcgg
     7681 cggtcccagt taacgaaaac cacgtacatc aggagctggt gtggaaagcc gatgttcatg
     7741 aggggaacag tcgcgacgtg gaagacaaat ccgattgcga ggatcaacca gcgcagccga
     7801 cgaaagaata tcgcgagcgg aaagagcagc tcccagacga gtataaaacc caccgcatac
     7861 ggcagatatt cccgcacctg cggtagccag aacgcgccac ggaaagcagt gtcgtgcagg
     7921 ttatcgagcg aatgaaagat cgcgagggcc ggtagattgt ctgtgcttaa atacttccac
     7981 ccagccacac gaagcttgat cagcgccgag ctgaaataga cccacgccag caacagttgc
     8041 atcaacagaa tcggatacgc gtaggcaaaa gcgggcggct gtgatttggt tttggcgaaa
     8101 cgactgtcga ttgaaaacgc gtcgccgcag ggagtaaacg cgagcaccac gaggaagtag
     8161 acggcgatca tctcgtcgtg gttgaagtgg ccgaagctac gcagcaggcc ctgataaaac
     8221 aacaccagca acagcgaagt cttggtggag atgatagtga agagtccggc tgtacttaag
     8281 gcgagcgaca gcagcatcag cgttttcaac gtcaacattc cgccctgtgt ttgtagcgcg
     8341 tcgtaaacac gccacggaaa aaacttcatc acaccggtgg gacgaagaat ggtgggcggc
     8401 aacgaaccga gtgcggagta ggaagtgacg acggtgtcaa agagaaatat gccgtgcacg
     8461 aagaaacgca cgataccaag cgcataggga ctcgacggaa cgacgcgatt tacaattcct
     8521 tgcgccacga ttctataaac ttgtcgtagt cagcatagtg accgccttcc cagcgataca
     8581 tgtcgagccg gatcgccttg agacgctttg gtgacgactc gggctcgcgc ttgttgtaga
     8641 tgttgcccca cgcctggagc agatccgata tgcgcgcgcc gggtggaagg ttctcgatgc
     8701 cgccgaactg acgcagcatc tcttcgttct gcggatgaat cgatttcagc ttgaacgcct
     8761 gattgtcgat cgtggcgttg accatggtcc aattgcggct gtaaagcgcg ttggtcaact
     8821 ggatagcagc gacgtcgatc gtctctccgg aaactgtttc gccgcgcagg atgcagtagc
     8881 tgcgaccgcg ctcgagatct ccgcccgcca tcatcacgtt ccacgccgtg atgggataaa
     8941 gattgcgcac cgcaacgaac gcccagaaca gtggcaggaa aagggcggcc gtgagaatgg
     9001 cgtacttgat gacgagtcgc ttggcgtcgt cgtcagcttg cgcccaggag tttgtaagtt
     9061 tggaaattat cgacaatgcg ctgcacgctc gaggtggcat ggtcagccgg gacttcgtaa
     9121 gtggcagtga tgtcggcgac gatctcgccc atcgacttac ccttttcgag tcccttccag
     9181 accaccatcg ccgtctcgtt cagttgataa tattttttcg tgttgagatc tacgagtatt
     9241 ccctcgccgc cgtcgaaatc tgtggcgaca acgtgtttgc ctggccttag ttcgttcata
     9301 tatttcacgg gttcgagatt cgcttttgtt ccagcagtcg atacggctcc cccgcccatc
     9361 gctcactcat ttcgcga
//
LOCUS       DASSFQ010000166        20882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1851017, whole genome shotgun sequence.
ACCESSION   DASSFQ010000166 DASSFQ010000000
VERSION     DASSFQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20882
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851017"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..788)
                     /locus_tag="VFH96_05685"
     CDS             complement(57..788)
                     /locus_tag="VFH96_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973495.1"
                     /translation="MNKQKSLLQGLPAALLCLSAAVSIYAQNPDGAKHNARPQQSVPA
                     STVAATKTDTAKYSYEFSQPKFYVKHIVIQHDANGRGTVTFERLNEDVPVTEPLELSP
                     DALARINTAWQGLRFLDSDTNYQADKQFPHLGTMKIGMERGDRKRVAEFNWTNNRDAE
                     TLVNEYRKAADQVILVFDIGIARENQPLNAPKLMETMESLLKRNGLSDPRQLLKLLQD
                     LSTDEHVPLIARNHALRLIKQIEKK"
     gene            complement(785..4663)
                     /gene="smc"
                     /locus_tag="VFH96_05690"
     CDS             complement(785..4663)
                     /gene="smc"
                     /locus_tag="VFH96_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET6973496.1"
                     /translation="MFKLERLEITGFKSFADYTEIIFTGDGITAIVGPNGCGKSNVAD
                     AIAWVLGEQRVKHLRGGEMKDVIFQGARNRPPSGMAEVVMHMIRDEVDEVEPDIEDID
                     STLERIDDRLVNIDEILPPEQVEAAADAAPPTEPSAVAPGNTDQPAPDASIPEPTPTQ
                     KITHKRHWRRRNLALEFAPGEAVSITRRLYRSGESEYLLNGKICRLRDIQDLFSGTGL
                     AGGHYAIIEQGRIGQILSAKPMDRRTIIEEAAGITKFRVRQRATEARLESARSNLSRI
                     SDIVSEIDRQVNSLRRQAAKARRYGVLREELRELLRLVYVAEDQKLATVLEDTNLKLS
                     EITELELGIADDLNKREEAARAATHEARSLEDELSTARAAAAEAVLRRDRQARECVYQ
                     RDQLSEIEKRKGEVTAEIEALTSRLVLIEAECRRLQQEDARLREEFDQSALVLRTAEE
                     SYAEKLSASNAAEIEIESSRAELLSQTAIAERVREIARQLETTLERLSQQAESLAREG
                     DRAASQHAERKLEAERYARELGDARARVAKLQAEREMAVDAVVRGHEAVSDTEAELTR
                     VRDEHSRASHRLESLKELDQRRAYYSPAVQLIFSPTETPRDFHFIGTLADALNVDAKW
                     ERAVEGVLGSSLQSIVVPTPDDAVRAAQWLRENNGGRASFLVAGLHGGSEDVEEVDTL
                     TALTCRLEERPVLLLTSGSTEPVREDLRISDLLGAPQQLVTVLKRTLPQKMDARIAAN
                     LEDAMVKSIATGEMYVTLNGDWIASGQFVNAGDARDLEEGAGLLAFKRELRELETRAE
                     ALAREVDTVANSAKDARARMVELEDTVVLLNEQIGREEREALAHEHTTTSLEQEVERA
                     ERHMRVVGQDSTRVDEERHEIENRRAQALLEAEAAEAAREAAADKVIKASASLTDLRR
                     EAEIESDSLAQQRATAAAASERRRATTAELRRIEGELADFAARVDRHRMELAEMGGRI
                     DELTLSIGDLELAASTVESDRAREEEQISDLSTRLQEARRQADEFSAELSELNRRAAE
                     VRDSRGGLEVQRAEAHARQTFVHENCQTELGHSLEELARELTFPEEFDLEVSHTRVEE
                     LRTRLEGFGGVNMMALEELAEAEQRLLFLTVQRQDIIEGISATEEALREIKRRSRERF
                     RHAFEEINKNFSSLFLELFGGGRGEMSLIDADDILESGIDVVAQPPGKRLQNVLLLSG
                     GEKAMAALALVLGIFHYRPAPFCLLDEVDAPLDEANVGRFTDKVVQMSAHTQFIVITH
                     NKRTMEMARALYGVTMEEAGVSKLVSVKFE"
     gene            complement(4728..6401)
                     /locus_tag="VFH96_05695"
     CDS             complement(4728..6401)
                     /locus_tag="VFH96_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HET6973497.1"
                     /translation="MSTSSTKRGDKKASPKNRGGQGKYSRFETGTAVAADIPEKAWRI
                     GVIAIFLIAAVLRLYDLNLVPLHHDEGVNGNFLVRLVREGAYTYDPGNYHGPTLYYFA
                     AIIPWITKVLFGNTARDNYGLTTFTIRLVPVLFGLATIGLIFLLRRRLGTVTVLAAGL
                     MLAVSPGAVYLSRYFIHETLFVFFTLGIVVSIVRFYDERNAAYLIPAAASAALLFATK
                     ETAMISVAVLVIAFAVMWVYMRVTGGSAKEASISERVSLVIDEMGGRASLVINILIAL
                     TVFVALYLLFYSSFFTNYKGINDSLQTFAIWKKTGNEAHVHSAEQYLIWLVKQESPLL
                     FFGALGAAITVLRPKNSLALFCALWSFGLIAAYSLIPYKTPWLVLNFIVPLALIAGYT
                     IQAIYEMFGHVIGATLVGWLLVGMISLAALSVAGYQTIDLNFFNYDNDSEYYVYVYAH
                     TKRHTIDLVNEVERIAKENSGNTTGITIVSPDYWPLPWYFRNFTRVGYYGRMAASTEP
                     VIIANANQKDEVDTNFGQLYQQVPGPDPGGTFELRPGVNLILYQRRRGL"
     gene            complement(6370..8373)
                     /locus_tag="VFH96_05700"
     CDS             complement(6370..8373)
                     /locus_tag="VFH96_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973498.1"
                     /translation="MIASLLLALLVTLSGTVATYLYDENASLGARLCAGAALGLSALG
                     LISFVVASLIGLSGMAVFFSTLICCSPLAILTDANTLKRFQQDLAGISTGTRKLLRFD
                     SWAVIYFLFYFIVAVTLWKVFSRAVIEDETGLSTGLLNNFGDLPFHLSVITSFVFGNN
                     FPPEDPTYAGVRFTYPFLSDFVSAVFVRCGADLRESMFIENYLLGIAFVGLIHRWAIV
                     ILRDRLAALITPLIVLLNGGMGFTLLFEKAGSNEGGLFGVLMDLPPSFTVIPETTWRW
                     GNAISALLVPQRGFLMGLPLAVIAFTQWWIATDKRPPQPPEKSSKKTRATSKQKNVWQ
                     DQLEHTRFPLNTRRMIAAGVAAGLLPLVHAHSFVVVMAMAGCLALLQIRWREWFAFFA
                     AALIIAVPQLLWSTMHSAVNAGSFFAFEVGWDHGQENPVYFWFKNTGVFIPLLLTAVL
                     LKNGSYVVPRRLLVFYLPFTLCFIVPNFLKMAPWIWGNIKVLYYWWLASSPLVALLLA
                     RLWQQGMTRRVIAVVLFVCVTLAGALDVAGIALRSIKYQVFDADGVRFAELVKAQTTP
                     RSLIVHAPVHNTPVFLTGRRSLMGYPGHIWTHGLEFVQREGEIKRIYLGAPDAEQLIS
                     KYGIEYLVVGPQERIVTMVNDQFINRFQKVGEVGEYKLYKTGR"
     gene            8537..9637
                     /gene="ychF"
                     /locus_tag="VFH96_05705"
     CDS             8537..9637
                     /gene="ychF"
                     /locus_tag="VFH96_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET6973499.1"
                     /translation="MLRAGIVGLPNVGKSTLFNALTAQSSALAANYPFATIEPNVGVV
                     SVPDERLEPLARLVKTEKIVPATVEFLDIAGLVRGASKGEGLGNQFLANIRETDTVVQ
                     VVRCFEDENIVHVEGAVDPLRDIETIQIELALADLATVERRREKAQKNLKSGDKIARQ
                     ELDVLDKIQPALEQGRSARTVALTDDEKAVARNFFLLTTKPTIYAANVEESALADLES
                     NSMVNAVREIAAKEAAECVVICAQLEADLVALPPEERVDYLKSLNVDASGVDQLIKSA
                     YHLLGLMSFLTAGEKEVRAWTIPQGTRAQTAAGTIHSDIERGFIRAEIVSYNDLIAAG
                     SYAIAREKGLLRLEGKDYIMQEGDVVHFRFNV"
     gene            complement(9679..10008)
                     /locus_tag="VFH96_05710"
     CDS             complement(9679..10008)
                     /locus_tag="VFH96_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973500.1"
                     /translation="MLTPEQRLDRAERILIATIKSGRRTRSEWRFKINSLIDAQIRDE
                     AEWRVRTAKIDEQLSQNAAGQAQNQKAIAELRESQAELKESQKLTDKALREFIESLRK
                     NPNGNSD"
     gene            complement(10152..10358)
                     /locus_tag="VFH96_05715"
     CDS             complement(10152..10358)
                     /locus_tag="VFH96_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973501.1"
                     /translation="MKDARNVLRIIGMILLGVFVFAVVLPLALKVVGITLGIIIGLAV
                     LLIKVAVTLAIAYLVLVGIRALLR"
     gene            10544..11881
                     /locus_tag="VFH96_05720"
     CDS             10544..11881
                     /locus_tag="VFH96_05720"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET6973502.1"
                     /translation="MDRWNSPSLFSDDAPETGAPVEGVPLAERMRPRSLSEFVGQTHL
                     VGEGRILRRLLAGGGALPSLILWGAPGTGKTTLARLLADSSGLRFIALSAVFSGVKDL
                     REAIQQAKAEQRYGKRTVLFIDEIHRFNKSQQDALLHAVENGTVTLIGATTENPSFEV
                     TGALLSRARVLVLNQLSEADIKTILQNAITDNERGLARFNPSISDELLDRMAHSANGD
                     ARVALTALDAAVESTTPDANGVRTVSEQTVIEALGRAHYAYDKGGEEYYNLISALIKS
                     LRNSDVDAALYWLARMIEGGADPIYIARRLCILASEDIGLADPQAMVQAAAAADIVQL
                     IGLPEGLFPLSQATIYLARAPKSNAIKSAYFAAAADAEETARVPVPLHLRNAATPLMK
                     QVGYGKDYRYVHSDPAAQEEMECLPEQLRGRKYFSEEGLSEQEQRGETTGSDD"
     gene            11871..12698
                     /locus_tag="VFH96_05725"
     CDS             11871..12698
                     /locus_tag="VFH96_05725"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6973503.1"
                     /translation="MTTNRKSDKELAFLQDLFIAPDWGERFAELIDEHLTLPKEGEVL
                     YVNAGTGGHALALHERADDKLELLCVDENPECIELARAKATATSEKIFFGTTPVDNLN
                     LEDNRFDLVIGNASLVSRQRVRKMFSELVRVAAPGATIALTLPTYSSFGEFFSIYWEA
                     LHNNGLLDHESDLEQLITELLTVSEVEQLAEDEGLGDVKSFTRIEEFDFESGEQFLSS
                     PLVAEFLMRDWLALVPDDKRAALFDEISRLINEERHEAEFALSVKATLVVGQKAHSH"
     gene            complement(12745..12942)
                     /gene="tatA"
                     /locus_tag="VFH96_05730"
     CDS             complement(12745..12942)
                     /gene="tatA"
                     /locus_tag="VFH96_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET6973504.1"
                     /translation="MNQTILAFGIGGLGTPELVIIVLVIFLLFGATRLPQLAKALGQS
                     KKAFREGIEDGERSEDRKLEK"
     gene            complement(12983..13462)
                     /locus_tag="VFH96_05735"
     CDS             complement(12983..13462)
                     /locus_tag="VFH96_05735"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET6973505.1"
                     /translation="MCTERRAVVMIFKGVARAEEHNGHMLHSQKLSIHAPSVIRLTEY
                     IHIPFERRSLSRKNILLRDHSTCQYCGTQYPPSELTLDHVLPRSRGGESAWDNLVACC
                     KRCNHRKGNRTPEESGMHLLRRPRGFSLHVNRQIMRYLGRADETWRKYLFYESDTRE"
     gene            complement(13570..14274)
                     /locus_tag="VFH96_05740"
     CDS             complement(13570..14274)
                     /locus_tag="VFH96_05740"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET6973506.1"
                     /translation="MNLTNVYDAVVMLAYPQICVICGQSVEQRRFGVACETCWQGTRI
                     FADEERICLKCGMPDQERCGRCEEMAFTAARAAGIYEGALRESVLLLKRKPYLPQYVE
                     GLLTDVVRREPLSRSTRVIPVPLHPKRLKTRGFNQASIIGQTVSKALRLPLDEFSLVR
                     VSTTEKYRAGMDAKGRRDTVAGAFRASNPRLVAGEDILLVDDVFTTGATISACAETLM
                     AAGVRNVFVLTVARAR"
     gene            complement(14338..14706)
                     /locus_tag="VFH96_05745"
     CDS             complement(14338..14706)
                     /locus_tag="VFH96_05745"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET6973507.1"
                     /translation="MDKRRTKVFRDKLLDRREGLVGQVQAAELYSRERDAEATQDPAD
                     MAANAYTKELLVSMSDNDRQLLNLIDEALERIEASGYGKCVHCGEAVPDKRLDAVPWA
                     RHCVRCQDLQERGLLGDDED"
     gene            complement(14747..15205)
                     /locus_tag="VFH96_05750"
     CDS             complement(14747..15205)
                     /locus_tag="VFH96_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET6973508.1"
                     /translation="MRLRIIWTGKTRDARLRALVDDYAARLSHFVRCEVTELRELGRA
                     DKSGIERETKRISDALRPGAVTVLLDSEGAEWTSQQLAAHVQSWEGSGVKEVAFVIGG
                     PNGLASDFKARADKRWSLSRLTLTHEMARVLLFEQLYRAYTIIHGLPYQK"
     gene            complement(15251..16201)
                     /locus_tag="VFH96_05755"
     CDS             complement(15251..16201)
                     /locus_tag="VFH96_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6973509.1"
                     /translation="MIELFARSPGMREAVGLAERVSSTDANVLITGESGAGKDLLAAY
                     VHSQSRRATHPFIKIDCATLPGELLEAELFGYERGAFTGAVEAKAGRFEAADKGTVVL
                     DEIAHLSTDAQAKLLRVIERREFERLGGRKTIKLDARVIALTNVDLDAAVKNGRFRED
                     LFYRLNVINIRLPPLRERKDDLSQLIEHFLNHYSAKHGRDVQRVSPAARAVLKSYDYP
                     GNARELANIIERAVIVATGKKVEEADLPAGLSAAVSAQQRKTKPQSLAQLEASYISET
                     LAATGGNKAECARILGISRKNLYEKIARYRLSTDYTDGAD"
     gene            complement(16198..16665)
                     /gene="rsfS"
                     /locus_tag="VFH96_05760"
     CDS             complement(16198..16665)
                     /gene="rsfS"
                     /locus_tag="VFH96_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET6973510.1"
                     /translation="MKQNSTVKEQSLNPQPSVRATPQPTVADQLDERVLAALHAAAEK
                     KAIEPVVLDLREIASFTDYFLIVSGANERQVQAISDEVYETLKRAGHAALRVEGYKTA
                     EWILLDYGDFVVHVFEQKARKFYDLERLWRESKRVELPAEFSADGSGSLRRES"
     gene            complement(16643..17323)
                     /gene="nadD"
                     /locus_tag="VFH96_05765"
     CDS             complement(16643..17323)
                     /gene="nadD"
                     /locus_tag="VFH96_05765"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET6973511.1"
                     /translation="MYAQKRIALYGGTFDPVHAGHLEVARRVLQLFEIEKVLFIPAQM
                     APHKIGRPVTEPIHRYAMLALATQDDPQLSISTFELDAPDRRYTVDTVDHFQQVLGDA
                     TELFFIMGADSWSEITTWREWERLLSMTNHIVVTRPGYEPVTTHVGKVSDRIVDVRGG
                     KSPSRIEGAKKIFFTDVVMKDVSATSIRRLASEGRVEELAGLVPGPVLEYIKKYGIYR
                     IDEAKLNS"
     gene            complement(17327..18367)
                     /gene="obgE"
                     /locus_tag="VFH96_05770"
     CDS             complement(17327..18367)
                     /gene="obgE"
                     /locus_tag="VFH96_05770"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET6973512.1"
                     /translation="MFIDRAKIHVQGGDGGNGVTAFRREKFVPRGGPSGGDGGRGGDV
                     ILVADASLNTLLHLRYNPSHIAPRGLHGEGSNRSGRTGEDLLVRVPVGTQIFDFTTGD
                     LLHDLSHDGDRWLAARGGRGGFGNAHFTTSTNRAPRYHQTGSKGEELDLQLELKLLAD
                     VGLVGFPNAGKSTFISTVSAARPKIADYPFTTLEPHLGVVDLGDFRTFVIADIPGLIE
                     GAHTGAGLGDRFLRHIERTKLLLHLVDVSSVSGREAVTDYETVNHELASYNQDLATRP
                     QFVVATKIDSLDEPERLESLQQRAMSDNKPFFAISSATGAGVRELVNAMAAKLEELNA
                     ESKRAETVELAL"
     gene            complement(18437..18706)
                     /gene="rpmA"
                     /locus_tag="VFH96_05775"
     CDS             complement(18437..18706)
                     /gene="rpmA"
                     /locus_tag="VFH96_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET6973513.1"
                     /translation="MAHKKGVGSSRNGRDSQSQRLGLKKFGGERVLGGNIIARQRGTR
                     WKPGNNVGRGKDDTLFALIDGVVKFEDKGRSGKFVSVYPAEQTAA"
     gene            complement(18712..18990)
                     /gene="rplU"
                     /locus_tag="VFH96_05780"
     CDS             complement(18712..18990)
                     /gene="rplU"
                     /locus_tag="VFH96_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET6973514.1"
                     /translation="MSHAVIKAGGKQFLVTEGQTIRVPSMAADAGSSIELEPVISSGG
                     GDGKISATVVAHGKDQKIVVFKKKRRKQYKRTRGHRQGYTTLKIDSIG"
     gene            complement(19122..20504)
                     /gene="accC"
                     /locus_tag="VFH96_05785"
     CDS             complement(19122..20504)
                     /gene="accC"
                     /locus_tag="VFH96_05785"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6973515.1"
                     /translation="MAFNPRKFKKILIANRGEIACRIIWTCKEMGIKTVAVHSDVDRD
                     SLHVRFADEAACIGPAPSAQSYLNIPAIISTAEIFNVDAIHPGYGFLAESAYFAEICE
                     ACNIKFIGPPANVIRLMGDKIEARRAMHEAGLPIVPGSPGAVTSEEEGLKLAREIGYP
                     IIVKASAGGGGRGMRIVRSEGELGKALDTASTEAAAAFNDGSVYIEKYIEQPRHIEIQ
                     VLADEFGECIHLGERECSIQRRHQKLMEEAPSIVLTPEQRKTMGDAAVAACKKLGYSS
                     AGTCEFLLDKHGDFYFMEMNTRIQVEHPVTEMVTLADIVRNQIRIAEGEPLNYNQDDL
                     LIVGHAIECRINAENPETFAPSPGTITAFNLPGGPGVRVDTFVYSGYQVTPFYDSLIA
                     KVIVHARTRELAIARMKRALDVMVVEGIKTTIPLHQKIMDDPRFRAGDIGTDFMEYFL
                     ARNGKKAVSA"
     gene            complement(20576..>20882)
                     /gene="accB"
                     /locus_tag="VFH96_05790"
     CDS             complement(20576..>20882)
                     /gene="accB"
                     /locus_tag="VFH96_05790"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET6973516.1"
                     /translation="ATAPSGPAHPGTQATTAAAQDQDLHIIQSPIVGTFYRSPSPTAD
                     AFVKIGSNVEPDTVVCIIEAMKLMNEIQAETSGEVVKIYVENGQPVEYGQPLFGIQS"
BASE COUNT         4600 a         6187 c         5560 g         4535 t
ORIGIN      
        1 cgttacgttg ccgttttaac gcagaggacg cagaggtacg cagagggccg cagagcctac
       61 ttcttctcga tctgtttgat caaacgaagc gcgtggttgc gggcgatgag cgggacgtgc
      121 tcgtcggtgc tgagatcctg gagcagcttg aggagttggc gcggatctga caacccgttt
      181 cgcttcaaca acgactccat cgtctccatc agcttcggcg cattcaacgg ctggttctca
      241 cgtgcgatcc cgatatcgaa cacgaggatc acctggtccg ctgctttgcg atactcgttc
      301 acgagtgtct cagcgtcgcg gttgttggtc cagttaaact ccgccacccg cttgcggtcg
      361 ccacgctcca tgccgatctt catcgtgccg agatgaggaa actgtttgtc tgcctggtag
      421 tttgtatctg aatcgaggaa ccgcaacccc tgccacgcgg tgttgatgcg cgcgagggcg
      481 tcgggcgaga gctcgagcgg ctcagtcacc ggaacgtctt cgttcaaacg ctcaaacgta
      541 acggtgccac gtccattcgc atcgtgctgg atgacaatat gtttgacgta aaacttcggc
      601 tgcgaaaact catacgaata tttggcagta tccgtcttag tggcggcgac agtcgacgcg
      661 ggaacggact gttgtggccg tgcattatgc ttagcaccgt ccggattttg cgcataaatg
      721 gaaaccgccg cgctcaggca cagcagcgcg gcggggagcc cttgcagcaa tgacttttgt
      781 ttattcactc aaactttacc gataccagct tcgagacacc cgcctcctcc atggtcacgc
      841 cgtacaacgc gcgcgccatc tccatcgtcc gtttgttgtg cgtgatcacg atgaactgcg
      901 tgtgcgcaga catctggacg accttatcag taaagcgtcc cacgttcgct tcgtcaagtg
      961 gcgcgtcgac ctcatccaat aaacagaacg gcgccggacg gtaatggaag ataccgagca
     1021 cgagcgcgag cgccgccatc gccttctcac cacccgacaa caacaacacg ttctgcaaac
     1081 gcttgcccgg tggttgggcc accacgtcga tgcccgattc gagtatgtcg tcagcgtcga
     1141 tcaaactcat ctcaccacgc ccgccgccaa acaactccag gaacaacgag ctgaagttct
     1201 tgttgatctc ttcgaacgcg tgacggaaac gctcgcgcga gcgacgcttg atctctcgca
     1261 gtgcttcttc agtcgcagaa attccctcga tgatgtcctg gcgctggacc gtcaggaaca
     1321 acaaccgctg ttctgcttcg gcgagttctt cgagcgccat catgttcaca ccgccaaagc
     1381 cctcgagtcg cgtgcgcagc tcttcgacgc gcgtgtgact tacttcgaga tcaaattctt
     1441 ccgggaacgt gagctcgcgt gcgagctctt ccaacgaatg gcccagctcc gtctggcagt
     1501 tctcgtgaac gaacgtttgc cgtgcgtggg cttcagcacg ttgcacttcg agaccgccac
     1561 gcgagtcgcg cacttcagca gcgcgtcgat tcagctctga caactcggcg gagaactcgt
     1621 ctgcctgccg gcgtgcttcc tgtagtcggg tcgagagatc cgagatctgt tcttcttcgc
     1681 gagcgcgatc gctttcgaca gtgctggcgg ctaactcgag atcgccgatc gagagtgtca
     1741 actcatcaat gcggccaccc atctcagcga gctccattcg atgtctgtcg acacgcgcgg
     1801 cgaagtcggc cagctcacct tcaatgcgac gcaactcagc agtcgtagcg cgacgacgtt
     1861 cggacgcggc agccgcggtc gcacgttgtt gcgcgagcga atcgctttct atctcggctt
     1921 cccgtcgcag atctgttagc gatgcagagg ctttaataac tttatcagct gcggcttctc
     1981 gtgctgcttc ggcagcttca gcttccaaca aagcctgggc gcggcggttt tcaatttcgt
     2041 gacgttcttc gtcgacgcgc gtcgagtcct gtccgacgac gcgcatgtgg cgctcggccc
     2101 gttcaacttc ctgctcgaga ctcgtcgtcg tgtgctcgtg agccagcgcc tcgcgctctt
     2161 cgcgaccgat ttgctcgttc aacagcacga ctgtgtcttc aagctcgacc atacgcgcgc
     2221 gcgcatcctt cgccgagttc gctacggtgt caacttctcg cgcgagtgct tcggcgcgtg
     2281 tttccaattc tctcagctcg cgtttgaagg cgagcaatcc ggcgccctct tcgagatcgc
     2341 gtgcgtcgcc cgcgttaacg aactggccgc tcgcgatcca gtcgccattc aacgtcacgt
     2401 acatctcccc agtcgcgatc gacttgacca tcgcgtcttc gagattcgcg gcgatacgcg
     2461 cgtccatctt ttgcggaagc gtgcgcttga gaacggtgac gagttgctgc ggcgcgccga
     2521 gcagatcgga gatgcgcagg tcttcgcgaa cgggttccgt agaaccgctg gtcaagagca
     2581 gcacgggacg ctcttcgaga cggcacgtga gcgccgtgag cgtatcgacc tcctcaacat
     2641 cttcactgcc gccgtgcaaa ccggcgacaa ggaaactcgc acgaccgcca ttgttctcac
     2701 gcagccactg cgccgcgcga accgcgtcgt caggcgtcgg cacgacgatc gactgtaaag
     2761 acgatccgag cacaccttcc acagcacgtt cccacttcgc atcgacgttc aacgcatcgg
     2821 caagcgtgcc gatgaaatga aaatcgcgcg gtgtttcagt cggagaaaag atgagctgca
     2881 cggccggaga ataataggcg cgccgttggt ccaactcctt caagctttcg agccggtgac
     2941 tcgcacgcga atgctcatca cgcacgcgcg tcagctctgc ctcggtgtcg ctgaccgctt
     3001 cgtgaccgcg gacaacggca tcgacagcca tttcacgttc ggcttgcagc ttcgccacac
     3061 gcgcacgcgc atcacccagc tcgcgtgcat acctttcggc ttcgagcttg cgctcggcat
     3121 gctggctggc ggcgcgatcg ccctcgcgcg caaggctctc ggcctgctgc gacaagcgtt
     3181 cgagcgtcgt ctcgagctgt ctggcaatct cgcgaactct ttcggcgatc gcggtttgtg
     3241 acaatagctc ggcgcgtgag ctctcgatct cgatctcggc ggcgttcgaa gcggacagtt
     3301 tttccgcgta cgactcctca gcagtgcgca acacgagcgc gctctggtca aactcttcgc
     3361 ggaggcgagc gtcttcctgt tgcaggcgac ggcattcagc ttcgatcaga acgagtcgcg
     3421 acgtcaacgc ctcgatctcg gcagtgactt cacccttgcg cttctcgatc tcactgagct
     3481 ggtcacgttg ataaacacac tcgcgtgcct gtcgatcgcg tcgcagcacc gcttcggcag
     3541 ccgcggcacg agcagtcgac aactcatctt cgagactgcg cgcttcgtga gtcgcggcac
     3601 gcgccgcttc ttctcgtttg ttcagatcgt cagcgatacc gagctcgagc tcagtaattt
     3661 cggagagttt taaattcgtg tcttccaaga cggtggcgag cttctgatct tcggcgacgt
     3721 atacgagacg cagcaactcg cgcaactctt cacgcaacac gccataacga cgagccttcg
     3781 cggcctggcg tctgagactg tttacctgtc gatcgatctc agaaacgatg tcggagattc
     3841 gagacagatt tgaacgcgcg gattcgagac gggcttcggt tgctcgctgc cgcacgcgaa
     3901 acttcgtgat gcccgccgct tcctcgatga tggtgcggcg gtccatcggc ttcgcggaaa
     3961 gaatctgtcc gatgcggccc tgttcgatga tggcgtagtg accacccgcc aaacccgtac
     4021 cggaaaacag atcctgaata tcgcgcagtc ggcagatctt tccgttcaat aagtattcgc
     4081 tctcgcccga gcgatagagc ctgcgtgtga tggaaactgc ttcgccgggc gcaaactcga
     4141 gcgcgagatt gcgacgacgc cagtgtcgct tgtgcgtgat cttttgtgtg ggagttggtt
     4201 ccggaatact ggcgtcgggc gcaggttggt cggtgttacc cggcgcgacc gcgctcggtt
     4261 ctgttggtgg cgcggcgtcc gctgctgctt ccacttgttc cggcggcagg atctcatcga
     4321 tattgacgag gcgatcgtca attctctcga gcgttgaatc gatgtcttcg atgtcaggct
     4381 cgacctcgtc aacttcgtcg cggatcatgt gcatgacgac ttccgccatg ccgctcggcg
     4441 gacgattgcg tgcgccctga aagatgacgt ctttcatctc gccgccgcgc agatgcttaa
     4501 cgcgctgctc gccgaggacc cacgcgatcg cgtcggcaac attgctcttg ccgcagccat
     4561 tcgggccaac gatggcggtg atcccgtctc cggtgaagat tatttcggta tagtcggcga
     4621 aggatttaaa cccggtgatt tcgaggcgtt caagtttgaa cattacgggg aagccactat
     4681 aagtgggagc caccccgaat gtcaaccaca acatgtattt agcctagcta aagacccctg
     4741 cggcgttgat aaaggattag attgactccg ggtcggagct caaaggtccc gcccggatca
     4801 ggaccaggta cctgctggta aagctgcccg aagttcgtgt cgacctcgtc cttctggttc
     4861 gcattcgcaa tgatgacggg ttcagtcgac gcagccatcc gcccgtaata tcccacgcgc
     4921 gtgaagttgc ggaagtacca tggcagcggc cagtagtcag gactcacgat cgtgataccc
     4981 gtggtgttgc cggaattctc cttcgcgatc cgttctactt cgttaaccag gtcgatagtg
     5041 tgccgtttcg tgtgagcgta aacgtaaacg tagtactcgc tgtcattatc gtagttgaaa
     5101 aaattcaaat cgatggtttg atagccagcc acactcaacg ccgctaacga aatcataccg
     5161 accagcaacc agcccactaa agttgctccg ataacgtggc cgaacatctc gtagatagcc
     5221 tgaatcgtat acccggcaat gagcgctagc ggcacgatga aattcagtac cagccagggt
     5281 gtcttgtacg gaatcagcga gtaagcagcg ataagaccaa acgaccataa cgcacagaac
     5341 agcgccaacg aattcttcgg cctcaaaacc gtgatcgccg ccccaagcgc gccaaagaac
     5401 aacagtggcg actcctgttt cacgagccag atgaggtact gttcggccga atgcacgtgc
     5461 gcttcgtttc ccgtcttctt ccaaatggca aacgtttgga gtgaatcgtt gatgcctttg
     5521 tagttggtga agaacgatga gtagaagagc aggtaaaggg caacaaagac ggtgagcgca
     5581 atcaggatgt ttattacgag gctggctcga ccacccatct cgtcaatcac taagctcact
     5641 cgctccgaga tgctagcttc cttcgcggaa ccgccagtga cccgcatata aacccacatg
     5701 acggcgaaag cgatgactaa aactgccact gaaatcatcg cggtttcttt ggtcgcaaaa
     5761 agaagcgccg ccgaagccgc cgccggaatg agataagccg cgttgcgctc gtcgtagaac
     5821 cgcacgattg acacaacgat tcccaaagta aagaacacaa acagcgtctc gtgaatgaaa
     5881 taacgcgaga gataaaccgc gccgggcgac acggccagca tcaatccggc tgcgagtacc
     5941 gtgacggttc ccaaccttct tcgcagcaga aaaatgagac caatcgtcgc cagaccgaag
     6001 aggaccggca cgagccggat cgtgaaagtc gtcagaccgt aattgtcgcg cgcagtgttg
     6061 ccgaatagta ctttggtgat ccagggaatg atcgcggcaa agtagtagag ggttggacca
     6121 tgatagttac ccggatcgta cgtgtaagct ccctcgcgaa cgagccgcac gaggaagttt
     6181 ccgttaactc cttcgtcgtg gtgcagcggc acgagattga ggtcgtacaa acgcagcaca
     6241 gcggcgatca ggaagatcgc aatgactccg atgcgccatg ccttttccgg aatgtctgcg
     6301 gcgacggccg tgccagtctc gaagcgcgag tatttaccct gaccgccgcg gttttttggc
     6361 gacgcttttt tatcgccccg ttttgtagag cttgtactca ccaacttctc caactttctg
     6421 aaagcgattg atgaactgat cgttaaccat cgtcacgatc cgctcctgtg gtccgactac
     6481 aagatactcg atgccatact tcgagattaa ctgctccgca tcaggcgcgc cgagatagat
     6541 gcgcttgatc tcgccttcgc gctgaacaaa ctcaagcccg tgtgtccaaa tgtgtcccgg
     6601 gtaacccatc aacgaacgtc gccccgtcag aaacacgggc gtgttgtgaa ccggcgcatg
     6661 aacgatcagg gagcgcggcg tagtttgcgc tttcacaagc tcagcgaagc gaacaccgtc
     6721 agcatcgaaa acctggtact tgatcgatcg cagggcaatg ccggcgacgt cgagcgctcc
     6781 cgccaatgtc acacagacaa aaagcacgac ggcgatgacg cgtcgcgtca tgccctgttg
     6841 ccacagtcta gccaacagca gcgccacgag cggcgacgat gcgagccacc agtaataaag
     6901 cactttgatg ttgccccaga tccacggcgc catcttcagg aagttgggaa cgatgaagca
     6961 gagggtgaat ggcagataga agactagaag gcgtctgggg acgacgtaac ttccgttttt
     7021 aagaagtacg gcggtaagca gcaaggggat gaagacgccc gtgtttttga accagaaata
     7081 taccggattc tcctggccgt gatcccatcc tacttcgaac gcaaagaaac tgcctgcgtt
     7141 cacggcgctg tgcattgtgg accagagtag ttgtgggacc gcgattatca acgccgccgc
     7201 aaagaacgca aaccattcgc gccagcggat ctgaagcagt gcgagacatc cggccatcgc
     7261 catcactacg acgaagctgt gcgcatgcac aagtggtaat aatcccgcgg cgacgccggc
     7321 cgcgatcatg cggcgtgtgt tgagcggaaa acgcgtgtgc tccaactgat cctgccagac
     7381 gttcttttgt ttgctggtcg cgcgtgtctt cttcgacgat ttttcaggcg gttgcggggg
     7441 ccgcttgtcg gtcgcgatcc accattgcgt aaaggcgatc accgcgagcg gaagtcccat
     7501 cagaaatccg cgttgtggaa ccagtagagc ggagatcgca ttgccccagc gccacgtggt
     7561 ttccgggatc actgtgaacg aaggtggcag atccatcagc acgccgaaga gtccaccttc
     7621 gttcgagccg gctttttcaa agagaagggt gaaacccatg ccgccgttca aaagaactat
     7681 cagcggcgtg ataagcgctg ccagccggtc gcgcagtatt acgatcgccc agcgatggat
     7741 caggccgaca aaagcaattc cgagaaggta gttctcgatg aacattgact cgcgcaagtc
     7801 ggcgccacac cgcacgaaca ccgcagagac gaagtctgaa agaaatggat aggtgaatct
     7861 gacgccggcg taagtcggat cttcgggtgg gaagttgttt ccgaaaacga aactcgtgat
     7921 cacgctgagg tgaaacggaa gatcgccgaa gttgtttagt agtcccgtgc tgaggccggt
     7981 ctcgtcctcg atcacggcgc gactaaacac tttccacaag gtcaccgcga cgatgaaata
     8041 aaaaaggaaa tagatgactg cccacgaatc gaatcgcagt agtttccgag tgccagtcga
     8101 aattcccgcg aggtcttgct ggaagcgctt caacgtgttt gcgtcggtga gtattgccag
     8161 cgggctacag caaatcagcg tgctgaagaa aacagccatg ccgcttaatc cgatcagtga
     8221 cgcgactacg aagctgatca gaccgagcgc cgacaggccg agcgccgcac ccgcgcacaa
     8281 ccgcgcaccc aacgatgcgt tctcatcgta gagataggtg gcaacggtac cgctcagggt
     8341 gactagtaac gccagtagta aggacgcaat catctatcgg agctgctgtc ttgggctgcc
     8401 aggtcgcttc ggattatacc catttcgaac ccttatccac atattacgca gattgttatt
     8461 tgaatctgtg taatctgcgg atgacctgct ttgcatcgtt cgcagtaatc ggttacgttt
     8521 ctgcgcgagg cgaataatgt taagagcagg catcgtcggg ctgcccaacg tgggtaagtc
     8581 gaccttattc aacgcgctca cggcgcagtc gtctgcactg gcggcaaatt atcctttcgc
     8641 gacgatcgag ccgaacgtcg gtgtggtttc agttcctgac gagcgtctgg aaccgctggc
     8701 gcgactggtg aagacggaga agatcgtgcc ggccacggtc gagtttctcg acattgccgg
     8761 gctcgttcgc ggcgcgtcga aaggcgaagg gctgggaaat cagtttctcg cgaatatcag
     8821 ggaaacggat acggtcgtgc aggtagtgcg ctgttttgaa gacgagaaca tcgttcacgt
     8881 tgaaggcgcg gtcgatccgc tacgcgatat cgagacgatt cagatcgagc tcgcgctcgc
     8941 ggatctagct accgtggaac gtcgacgcga gaaggcacag aagaacctca agagcggaga
     9001 caagatcgca cgacaggagt tggatgtgct ggacaagatt caacccgcgc tcgagcaggg
     9061 acgctcggcg cgaactgtcg cgctcactga tgatgagaaa gctgtcgcgc gtaacttttt
     9121 tctgctaacg acgaagccga cgatctatgc agcgaatgtt gaggagtcgg cgctcgctga
     9181 tctcgagagt aattcgatgg tgaatgcagt acgtgagatc gcagcgaagg aagcggcgga
     9241 gtgtgtcgtc atctgcgcgc agctcgaagc cgatctggtt gcgttgccgc ctgaagagcg
     9301 cgttgactat ttgaagagtt tgaatgtaga cgcgagcggt gtggaccaac tgatcaagag
     9361 cgcgtatcac ctgctgggct tgatgtcgtt tctgactgcg ggtgagaaag aagttcgcgc
     9421 ctggaccatt ccgcagggca cacgcgcgca gaccgcagcg ggcaccattc attcagacat
     9481 cgagcgcggc ttcatccgcg ccgagattgt cagctacaac gacctgatcg ccgccggctc
     9541 ctacgccatc gcccgagaga aaggcctgct acgcctcgaa ggaaaagact acatcatgca
     9601 ggaaggcgat gtagttcatt tccgattcaa tgtttagcca caaaggcaca aagaaagaag
     9661 aggcttggtg ctatttgttt aatctgagtt gccgtttgga ttcttgcgaa gactctcgat
     9721 gaactcccgc aatgctttgt cggtcaactt ctgtgattcc tttaactcgg cctgtgactc
     9781 ccttagctct gctatagctt tttggttctg agcttgacca gcggcattct ggcttaattg
     9841 ctcatctatc tttgccgttc ttacgcgcca ttcggcctca tccctaatct gggcatcaat
     9901 aagactattg atcttaaacc gccattcgct tcgagtgcgc cggccggatt tgatcgttgc
     9961 aattaagatt cgttctgctc gatcgagtcg ttgttctggt gtcagcattt cgtccatcct
    10021 tcaggtgagg tgaaaaatca gcggcatgat cgcatgtgcg tataactccg tcaactcaag
    10081 ccgatcaaaa agcacaaaag gcacaagaga gaatgattct cttttgtgcc ttttgtgcct
    10141 tttgtggcta actaccgtag caaggcacga ataccaacaa gcacgaggta tgcaatcgcc
    10201 agtgttaccg ccaccttgat caacagtact gccagtccaa ttatgatgcc caatgtaatt
    10261 cccaccactt tcaacgccag cggcaggaca actgcaaaaa caaatacacc cagaagaatc
    10321 attcctatga ttctcagaac gtttctcgca tccttcatcg tctgctctcc attcgctttc
    10381 gcgagcccca aattgcttcg gtttatcgct cgcagccaac tatacgcaga ttctcacaat
    10441 cgcgttccaa tgaagattct gaatacttta cccgtaaaaa tggctaccca gccagtcgct
    10501 tgtttgattc ttttcggcga taattataca cactttttgc tccatggacc gctggaattc
    10561 accaagcctg tttagcgacg acgccccaga gaccggggcg ccggtcgagg gcgtcccgct
    10621 cgccgagcgc atgcgaccgc gctccctgtc ggagtttgtc ggccagacgc atctcgtcgg
    10681 cgaaggccgc atcctgcgac gattgctcgc gggcggcggc gccctgcctt cgctgatcct
    10741 atggggcgcg cccggcacag gcaaaacaac tctggcccgc ctgctcgctg acagcagcgg
    10801 cctgcgcttc atcgcactct ccgccgtctt ctcaggcgtg aaagacttgc gcgaggcgat
    10861 tcaacaggcg aaggccgaac aacgctacgg caaacgcacg gtgctcttca tcgacgagat
    10921 ccatcgcttc aacaaatcgc aacaggacgc actgttgcac gcagtcgaaa acggcactgt
    10981 cacactgatc ggagcgacga ccgagaatcc gtcgttcgaa gtcacggggg cgcttctttc
    11041 acgcgcgcgt gtgctggtac tcaaccaact ctccgaagct gacattaaaa ctattctcca
    11101 aaacgccatc actgacaacg agcgtgggct cgcccgtttc aatccatcaa tctccgacga
    11161 attactggat cgaatggcgc actccgcgaa cggcgacgca cgcgttgcac tcaccgcgct
    11221 ggacgcagcc gtcgagtcaa ccacaccaga cgcaaacggc gtcagaacag tttccgagca
    11281 aaccgtgatc gaggcgctcg gtcgcgcaca ctacgcctac gacaaaggcg gcgaagaata
    11341 ctacaacctg atctccgcgc tgattaagtc tttgcgtaac tcagacgtcg acgcggcgct
    11401 ctactggctc gcgcggatga tcgaaggcgg cgccgatccg atctacatcg cgcgacgttt
    11461 gtgcattctc gcttccgaag acatcgggct cgcggatccg caggcgatgg tccaagccgc
    11521 tgccgctgcc gatatcgtgc aactgatcgg tttgcctgaa ggactctttc cactctcgca
    11581 ggcgacgatc tatctggcac gcgcacctaa gtcgaacgca atcaagagtg cgtatttcgc
    11641 agcggcagca gacgcagaag aaacagctcg cgtgccagtg ccgctgcatt taagaaacgc
    11701 agccacgccg ctgatgaaac aagtcggtta cggcaaagat tatcgttacg tgcacagcga
    11761 tccagccgcg caggaagaga tggagtgttt gcccgaacaa ctgcgtggta gaaaatattt
    11821 ttcagaagaa ggtttatcag aacaggaaca aaggggtgag acaacaggaa gtgacgacta
    11881 acaggaagtc tgacaaggaa ctcgcattcc ttcaggattt attcatcgcg cccgattggg
    11941 gcgaacgttt tgcagaacta attgacgagc acctaacact tccgaaagag ggggaagtgc
    12001 tttacgtcaa cgccggtacg ggcggtcatg cgctcgcgct gcacgagcgc gcggacgaca
    12061 aactcgagtt gctctgcgtc gacgagaatc ccgagtgcat agagctcgcg cgcgcaaaag
    12121 cgacagcaac cagcgaaaag atcttcttcg gcactacacc agtcgacaat ctcaatctgg
    12181 aagacaatcg ttttgattta gtaataggta atgcatcact tgtttcgcgc caacgagtgc
    12241 gcaaaatgtt ttccgagctc gtgcgcgtcg cagcgcccgg ggcaacgatc gcgctgacac
    12301 tgccgactta ctcgagcttc ggcgagttct tttcgatcta ctgggaagca ctgcacaaca
    12361 acggattgct cgatcacgaa tcagacctcg agcagttgat caccgaattg ctgacagttt
    12421 ctgaagttga acaactagca gaagacgaag gcctcggcga cgtgaaatca ttcacgcgga
    12481 tcgaggagtt tgacttcgag tcaggcgagc agtttctaag ctctccactc gtagcggagt
    12541 ttctgatgcg cgattggctg gccctcgtac ctgacgataa gagggccgca ttgttcgatg
    12601 agatctctcg gttaataaac gaagagaggc atgaggcgga gttcgcgctt tccgtgaaag
    12661 caactttagt cgtcggacag aaggcccata gtcattgaaa acgaaggcgg gagacttcag
    12721 cactgggcca tgtccgcacg tgcgctactt ctccagctta cggtcctcgc tgcgctcacc
    12781 atcttcgata ccctcacgaa aagccttctt gctttgtccg agggctttcg ccagttgcgg
    12841 caaccgggtg gcgccgaaaa gcaaaaagat cacgagcacg ataattacga gttcgggggt
    12901 gccgagtcct ccgatcccga aagccaggat tgtctgattc ataatttcct ctttctaacg
    12961 ggccgcgccg ggtggggccg aattactctc tagtgtctga ttcgtagaac aggtacttcc
    13021 tccaggtctc gtcagctctg cccaggtaac gcatgatctg tcgattcacg tgcagtgaaa
    13081 acccacgagg tctgcgcaac aggtgcatgc cggattcctc tggagtccgg ttaccctttc
    13141 gatgattaca acgcttgcaa cacgctacca gattatccca cgcggactcc cctccgcgcg
    13201 agcgtggaag cacatgatcg agcgttaatt ccgaaggcgg gtactgagtg ccgcagtact
    13261 ggcacgtcga atggtctcgc agcaggatgt tcttgcgcga gagcgagcga cgttcaaaag
    13321 gtatgtggat gtattccgtc aagcgaataa cagaaggtgc atggatggac agtttttgcg
    13381 aatggagcat gtgaccattg tgctcctccg cccgcgccac tcccttgaag atcatcacga
    13441 cggcgcgcct ttcagtgcac acattgatgg gctcaaagga agtattaagg actaaaactc
    13501 tgccattcat tagcgcgata ttacgcgagc gcggaagagg gtgtcaaaat aactttgggt
    13561 aaactcggcc taccgcgccc gggcgactgt cagaacgaag acattccgaa ccccagccgc
    13621 catcagggtt tcggcgcagg ccgagatggt ggcgccggtc gtaaacacgt catctaccag
    13681 caaaatatcc tcacccgcga ccagccgcgg gttgctagcg cggaacgctc cagccacggt
    13741 atcccgccgc cctttcgcat ccatgccggc gcggtatttt tccgtagtcg agacacgcac
    13801 aagactgaat tcatcgagcg gtaacctcag cgccttcgat accgtttgcc cgatgatcga
    13861 tgcctgattg aaaccccttg tctttagccg tttcggatgc agcggcaccg gaatgactcg
    13921 cgtactgcga ctcagcggct cacgccttac tacatcagtc agcaaccctt caacgtattg
    13981 cggcaagtaa ggctttcttt tcaacaacaa caccgactcg cgcaacgcgc cctcgtaaat
    14041 cccggccgcc cgcgcagccg tgaacgccat ctcctcacac ctgccgcacc gctcctgatc
    14101 cggcatcccg cacttcaaac aaatccgttc ctcatccgcg aagatccgtg ttccctgcca
    14161 acacgtctca cacgcaactc cgaatcgtct ctgctcgacg ctttgtccgc agattacaca
    14221 gatttgcgga tatgcaagca tgactactgc atcgtagaca ttcgtgaggt tcatacttaa
    14281 acaaagcggc ctccccgaat cgaggaagcc gcaaattatc tcaaactaac accaaactta
    14341 atcttcgtcg tcgcccaaca acccgcgctc ctgcaagtcc tggcagcgca cgcaatgtcg
    14401 cgcccacggc accgcgtcga gacgcttatc cggcactgct tcaccacagt gcacacactt
    14461 tccataaccc gaagcttcga tacgttcaag cgcttcatcg atgagattca gcaactgccg
    14521 atcattgtca gacatcgaga ccagcagctc tttcgtgtat gcattagccg ccatgtctgc
    14581 cggatcctgc gtggcttctg catcgcgctc gcgactgtaa agctcagccg cctgcacctg
    14641 tcccacaagg ccttcgcgac gatccaataa cttgtctcga aaaacttttg tgcgcctctt
    14701 atccattatt tattttgacc ctccacccta cttgttattg ctaagatcac ttctgatacg
    14761 gcaaaccatg aatgatcgtg tacgctcgat ataactgttc aaataacagc actcgtgcca
    14821 tctcgtgcgt tagtgtcaag cgcgacagcg accaacgctt gtctgcacgt gctttgaaat
    14881 cgcttgccaa accgttaggc ccgccgatga caaaggcgac ttccttaacc ccgcttcctt
    14941 cccagctttg cacgtgagca gcgagttgct gcgacgtcca ctcagcaccc tcggaatcca
    15001 aaagcaccgt gaccgcgccg ggacggagag cgtctgagat gcgtttcgtc tcccgttcga
    15061 tgcccgactt gtcggcgcgt ccaagttctc gcaattcagt cacttcacaa cgtacaaaat
    15121 gactgagtcg cgcggcgtaa tcgtcaacga gcgcgcgcag tctcgcatcg cgagtcttcc
    15181 ctgtccagat gatccgcaag cgcatcagtt atccacagat tacacagatt acgcagactc
    15241 ttttcgctga ttaatctgcg ccatctgtgt aatctgtgga taaccgatag cgcgcgatct
    15301 tctcgtagag attcttgcgg ctgattccga gtatgcgcgc gcactctgcc ttgttccctc
    15361 cggtagccgc aagcgtttct gaaatgtaag acgcttccag ctgcgccaga ctctgcggct
    15421 tcgtctttcg ctgttgcgca ctcaccgcgg cgctcaatcc cgccggtaaa tccgcttctt
    15481 caactttctt cccggtggcg acgatcaccg cgcgctcgat gatgttcgcg agctcgcgcg
    15541 cattgccggg atagtcgtaa gacttgagca ctgcccgcgc cgccggtgac acacgctgca
    15601 catcgcgccc gtgcttcgcg gagtagtgat tcagaaaatg ctcgatcagt tgtgaaagat
    15661 cgtccttgcg ctcacgcagc ggaggcagtc ggatgtttat tacgttcagt cgataaaaaa
    15721 ggtcttcccg aaaacgacca ttcttcacag ccgcatcgag atcgacattc gtcaacgcaa
    15781 tcacacgcgc atccagcttt atggtcttgc gcccgccgag tcgttcaaac tcacgacgct
    15841 cgatcacgcg caacaacttt gcctgcgcgt cggtcgagag atgcgcaatc tcgtcgagca
    15901 cgacagtgcc tttatcggca gcctcgaagc gcccggcctt tgcttcaacc gcgcctgtaa
    15961 acgcaccgcg ctcgtaaccg aatagctcgg cttcgagcaa ctcgccgggc agcgtcgcgc
    16021 aatcgatctt tatgaacggg tgtgttgcgc gtcgcgactg cgagtgaacg tacgcggcca
    16081 ggagatcctt tcccgccccc gactcacccg taatcagaac gttcgcatcg gttgaactta
    16141 cgcgctccgc gagaccgacc gcctcgcgca ttccgggaga gcgcgcaaat agttcgatca
    16201 cgattctctt ctcaaagaac cactaccatc cgcgctaaat tccgccggca gctcgacgcg
    16261 cttcgactcg cgccagagac gctcgaggtc gtagaacttc cgcgccttct gctcaaacac
    16321 gtgcactacg aaatcaccgt aatccaaaag aatccactcg gcggttttgt agccctcgac
    16381 gcgtagcgcc gcatggcccg cccttttcag cgtctcgtaa acctcgtctg aaatcgcctg
    16441 cacttgccgc tcgttcgcgc cactcacgat caagaagtag tcagtgaaac tggcaatctc
    16501 gcgcaggtcc aaaacgaccg gctcgatcgc tttcttctcg gcggctgcgt gaagtgcagc
    16561 gaggacgcgc tcgtcgagct gatcggcaac cgttggttga ggagtggccc gcacgctggg
    16621 ttgcgggttt aacgactgtt ccttaactgt tgagttttgc ttcatcaatt ctataaattc
    16681 cgtacttctt tatgtactca agcaccggtc cagggacgag accggccaac tcttccacgc
    16741 gaccttcact cgcgagacgt ctgatgctcg ttgccgaaac atctttcatc accacgtctg
    16801 tgaagaagat tttctttgca ccttcgatcc gcgagggcga cttgccaccg cgcacgtcaa
    16861 cgatgcggtc actgactttg cccacgtgcg ttgttacggg ttcgtagccg ggccgggtga
    16921 cgacgatatg gttcgtcatc gacagcaaac gttcccactc acgccaggtc gtgatctcag
    16981 accacgaatc ggcgcccatt atgaaaaaga gctcggttgc atcacccagc acttgctgaa
    17041 agtgatcgac cgtatccacc gtgtaacgcc gatccggagc atcgagctcg aaagtcgaaa
    17101 tcgataactg cggatcgtcc tgcgtcgcga gggcgagcat cgcatagcga tgtatcggct
    17161 ccgttacagg acgcccgatc ttgtgcggcg ccatctgggc cgggataaaa agtactttct
    17221 caatctcaaa tagctgtaaa actcggcgcg cgacttctag atgacctgca tgaaccggat
    17281 cgaaagtgcc gccatacaac gctatccgct tctgcgcgta catttcttac agagccagct
    17341 ccaccgtctc agcgcgtttg ctctccgcgt tcaactcttc gagcttcgcg gccattgcat
    17401 tcaccagctc acgcacgcct gcaccagtcg cagacgaaat cgcaaagaac ggtttgttgt
    17461 ctgacatagc acgctgctgc aacgactcca aacgctccgg ctcatcaagc gagtcgatct
    17521 tcgtagcgac aacgaactgc ggccgtgtag ccagatcctg gttgtacgac gcgagctcgt
    17581 gattcaccgt ctcgtaatca gtaaccgctt cgcgtcctga cactgacgaa acatcgacga
    17641 gatgcagcaa caacttcgtg cgctcgatgt gtcgcagaaa tcgatcgccc aaaccggctc
    17701 ccgtgtgcgc gccttcgatc aatccgggta tgtcggcgat cacgaaagta cgaaagtcac
    17761 cgagatcgac cacaccgaga tgcggctcca gggtcgtgaa tggatagtcc gcaatcttcg
    17821 gacgcgccgc ggagacagtc gagataaatg tcgacttccc agcattcgga aaacccacga
    17881 gcccgacatc cgctagcagc tttagctcga gctgaaggtc caactcctcg cccttgctgc
    17941 cagtctggtg ataacgaggc gcgcgattcg tcgaagtcgt gaagtgcgcg ttgccaaaac
    18001 cgccgcggcc accacgcgct gccagccagc gatcgccgtc gtgactgagg tcgtgcagca
    18061 agtcaccggt cgtaaaatca aagatctgtg ttcccactgg aacgcgaacc agcaagtctt
    18121 ccccggtccg gcccgagcga ttcgatccct ctccgtgcag accgcgaggg gcgatgtggc
    18181 tagggttgta acgcaggtgc agcagcgtgt tcagcgatgc atccgcaacg agaatcacgt
    18241 cgccgccgcg accaccatct ccgcctgatg gaccaccgcg aggcacaaac ttctcgcgcc
    18301 ggaaagctgt aacgccgtta cctccgtcac ccccttgcac gtgaatcttg gcgcgatcga
    18361 taaacatcta aattgaaaac gacgcccggg cgcgctttat tgctgctcag gcgtcgcgaa
    18421 ctttttcaga actgttctaa gcagcagttt gctccgctgg ataaacgctg acgaacttgc
    18481 cggaccgacc cttgtcttca aacttcacca caccgtcgat cagcgcgaac aacgtgtcgt
    18541 ctttcccgcg gccaacattg ttgccgggtt tccacctcgt gccacgctgg cgcgcgatga
    18601 tgttgccgcc gagcactcgc tcgccgccga atttcttcag accgagtctt tgcgactgcg
    18661 agtcgcgacc gttacgagat gaaccaacac cttttttgtg tgccattgtc tttacccaat
    18721 cgaatcaatc tttaacgttg tgtagccctg gcgatgaccg cgcgtgcgtt tgtactgctt
    18781 gcggcgcttt ttcttgaaga cgacgatctt ctgatctttg ccatgcgcta ccactgtggc
    18841 ggaaatcttg ccgtcaccac ctcccgagct gatcaccggt tcgagctcga tcgacgagcc
    18901 ggcatcggcc gccatcgagg gcacgcgaat cgtctggcct tccgtgacca ggaactgttt
    18961 tccaccggct ttgataactg cgtgtgacat cttaatcacc tcataaaaag tcgagcatta
    19021 taaagtattg cgcccaaaaa ccgcaaactc gatccaccga ttccgcagat tacactgaat
    19081 aaaataatct gcgtaatctg tgcaatctgt ggataaaacg gctacgcgct cacggccttc
    19141 ttgccgtttc gcgccaggaa atactccatg aaatccgtgc cgatatcacc ggcccggaaa
    19201 cgcgggtcgt ccatgatctt ctggtggagc gggattgtcg tcttgatacc ctcgaccacc
    19261 atcacatcga gcgcccgctt catccgcgca atcgcgagct cacgcgtacg cgcatgaacg
    19321 atcaccttcg cgattagcga gtcgtaaaac ggcgtcacct ggtaaccgga ataaacaaac
    19381 gtatcgacgc gcacgcccgg cccgcccggc agattgaagg ccgtgatcgt tcccggcgag
    19441 ggcgcgaatg tctccggatt ctcagcgttg atgcgacact cgatcgcatg tccgacgatc
    19501 agcagatcat cctggttgta attcagcggc tcgccttcgg cgatccggat ctggttgcga
    19561 acgatgtcag cgagggtcac catctcggtc accggatgct cgacctggat gcgcgtgttc
    19621 atctccatga aatagaaatc gccgtgctta tcgaggagaa actcacacgt gcccgcactc
    19681 gaataaccga gtttcttaca cgcagcgacc gccgcatcgc ccatcgtctt gcgctgctcg
    19741 ggtgtcagca cgatggacgg cgcctcttcc atcagtttct ggtgacgacg ttgaatcgag
    19801 cactcacgct cgccgagatg gatgcactcg ccaaactcgt ccgccagcac ctggatctcg
    19861 atgtgtcgcg gctgctcgat gtacttctcg atgtaaacac tgccatcatt gaacgccgcc
    19921 gccgcttcgg tcgatgccgt gtcaagcgct ttcccgagct cgccttccga acgcacgatg
    19981 cgcataccgc gaccaccgcc accggctgaa gccttgacga taatgggata accaatctca
    20041 cgcgcgagct tcagtccttc ttcttccgaa gtgacggcgc cgggacttcc cggcacgatc
    20101 ggcaaaccag cttcgtgcat cgcgcgccgc gcttcaatct tgtcgcccat caaacgaatc
    20161 acgttcgctg gtggtccaat gaacttgatg ttgcaagctt cgcagatctc agcgaagtaa
    20221 gccgactcgg cgagaaaccc gtaaccggga tggatcgcat cgacgttgaa gatctcagcc
    20281 gtgctgatga tggcggggat gttgagataa ctctgcgcgg acggcgctgg tccaatacac
    20341 gcggcctcgt cggcaaagcg cacatgcagc gaatcgcgat cgacgtcgct gtgaacggcg
    20401 accgtcttga tgcccatctc cttgcaggtc cagatgatgc gacaagcaat ctcgccgcga
    20461 ttggcgatca agatcttctt gaacttgcgc ggattaaaag ccatataaaa tctttgccac
    20521 aaaaggcaca aaaagcacaa aaaatcactt ttgtgctttt tgtgcctttt gtggcctaac
    20581 tctggattcc aaagagtggc tggccgtatt caaccggctg gccgttctca acgtaaatct
    20641 tgactacctc tccactcgtc tcggcctgaa tctcgttcat gagtttcatg gcttcgataa
    20701 tacaaacaac cgtgtcgggc tcgacgttgc tgccgatctt cacaaacgcg tcggccgtcg
    20761 gtgatggcga acgatagaac gtgccgacga tgggcgattg gatgatgtgt aaatcctgat
    20821 cctgggcagc agctgtcgtg gcttgtgttc ccggatgtgc cggacctgag ggcgcagtcg
    20881 cc
//
LOCUS       DASSFQ010000167         7360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_518277, whole genome shotgun sequence.
ACCESSION   DASSFQ010000167 DASSFQ010000000
VERSION     DASSFQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518277"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1073)
                     /locus_tag="VFH96_05795"
     CDS             complement(15..1073)
                     /locus_tag="VFH96_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET6973517.1"
                     /translation="MVTQRKPKASKYLTTPTRPVQVDRDRSVAGLLEKMEGTGFGGRQ
                     LAEAHRIWLDMLGDNTTIIVAASGALIPAGMRRLLAYVIKNRFVDVLVLSGSVLFHDL
                     HETLGRYHFQAHPSMTDAELESAQISRMWDLLASDEEYREAKEWIGGFTNQLDQTRAY
                     SPREFLHLLGRELAEIASEDGVLTSAYKARVPIFCPAIADSGLAVGIAGARFEKKNNF
                     MFDLVQDVLDMMNVMANARVSGILNLGGGTTKSFIQTAAMSSFMQKEHHHGHKYAIQI
                     STDAPHPGGRSNAVSFDEGLIYGKLARGAHTAYVPCDATIALPILITSLSQTAAKYMK
                     GRKKPNFTFGRELIVEAP"
     gene            complement(1136..3073)
                     /gene="speA"
                     /locus_tag="VFH96_05800"
     CDS             complement(1136..3073)
                     /gene="speA"
                     /locus_tag="VFH96_05800"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET6973518.1"
                     /translation="MTTAIEQTLETYGIENWGAGYFDVNRKGNLVVRPVEGDPRSADL
                     KEIIDDLAKRGITTPVLLRFPQLVAAQVRKLQRAFSRSIREFEYQGAHMCVYPMKVNQ
                     QRSVVEEYLREGSRYDFGLEAGSKAELYAALALEQPGDSLLVLNGFKDEEFIELAFAG
                     AKVGKKVVIVIEKLNELDHVLNVVERHEGALPMLGMRVKLYSKGSGRWEKSGGEAAKF
                     GLTTTELLEVIRRLEEADRMDMLKLLHFHIGSQLTDIKRIKNAMKEAARVYAKVYQLK
                     APIELLDVGGGMAVDYDGSKTAFDSSANYTAQEFANDVIYTIKQVCDDENVPHPTIIQ
                     ESGRFLAAYHAILVTNVLDEIETVVEDITPIEIDEDDPQVVTELGELRESITVKNYRE
                     YYHDALEHREELFTLFNLGLISLEDRAKGEVLFWDVCERADKYAQQAKYVSEEFGELR
                     RLLCAKYLTNFSVFRSVPDHWAINQLFPIVPIHHLNKPPTEYTTLCDITCDSDGIVDK
                     FVDLHDVKPVLELHDLNPDEPYYLAFLLVGAYQEAMGNNHNLFGAPHEAHIHIDEEGF
                     LIKKVIKGTTVGAAAERARYERSLLHDGFRRLITRRVKDGELSEADGAELAQFYESKY
                     EAYTYLAVNGVKKNSRRRTDY"
     gene            3491..4915
                     /gene="glnA"
                     /locus_tag="VFH96_05805"
     CDS             3491..4915
                     /gene="glnA"
                     /locus_tag="VFH96_05805"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET6973519.1"
                     /translation="MNPKTVLKYAADQGAKFVSVRFTDLPGSWQHLTFPIAELGEDQF
                     EDGFGFDASSIRGWAAIHESDMLLVPDASRFWMDPFAEQPTLCLIGSAVDPITKEGYA
                     FDPRSVAQRAESYLKFTGLADTAYFGPEAEFFVFDSVKFHNEQNSAGFTIDSDEGHWN
                     SGRDANEFAGSNLGYRIRPKEGYVPCAPTDSLTDLRSEISLTLGKCGVTVECHHHEVA
                     TAGQCEIDFRFSTLLGTADNLQIFKYVVRNTAHQFGKTATFMPKPLYGDNGSGMHCHQ
                     SLWKSEKPLFAGDGYAGLSDMAKFYIGGLLKHAAAIVAFAAPTTNSYKRLVPGFEAPV
                     NLAYSARNRSAAIRIPMFSANPKLKRLEFRPPDPSCNAYLAFAAMLLAGLDGVQNRID
                     PGDPLEKDIYDLSPEELKDVPTLPGSLDDSLKALEADHDFLLKGDVFSPQLIERWISY
                     KRDREITPLRMRPHPLEFSLYYDI"
     gene            4944..>7360
                     /locus_tag="VFH96_05810"
     CDS             4944..>7360
                     /locus_tag="VFH96_05810"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973520.1"
                     /translation="MNTDQSQSVFIRGIHDLPDQNGPRLFLERLAKEQSRAHQTLLKQ
                     PALLADVLALAAWSPLLGTTLEQNSEYLSWLGRERADPRVRTADEFKESLARFALTNS
                     SLNTQVLLARFRRRELLRTYLHDIRRSQTLVETMEELSNLADAILDFALSLARQDLDN
                     RYGLPRTVDDRGRSATADYCVIALGKLGSMELNYASDIDLVFLYSDDGTTSGSGQRGE
                     VSNREYFVKLSETIARLVGQSGGEGAAYRVDLRLRPHGRDGALACSLQEGVLYYMHTA
                     QDWERQALIRSRAAVGSAELFSRFKAAVTPAVFRPDVSITGALASVRLAKQKIDRKVE
                     KKSGFNVKLGRGGIREIEFIAQALQLAHGGLDDWLRVPHTLVSLGRLADRGFITQPER
                     SDLSDAYYFLRCLEHRLQMEHGLQTHSVPESEETQSLVGRRMGFAGADDFKAALKTHT
                     TNVRCTYDRLFSEAEETPTVSEEFSPALPEVGDKDTALALGAARVFVSYMDHRWENRP
                     RSLANYLLKLSARALNPHRALVQTSRIAASLEKTEHQFLISEANLDALLNLCGTSDSF
                     AETIAANPALIASLSAPTAATLRRDYRAILRTAIDAERSFPAELAALRLQWAEFMVEI
                     GARDARNELSIFEANHLQTDLATASMNVAYLIARREMARRYGSFSGGPRIAFYGLGRL
                     GTSGVDYGSDLDILITYDSLVPSPIKTLTQDETYSRLVELMIAALSSITREGYLYRVD
                     MRLRPHGKDGPIVTSSEGFLDYLKEQSAPWEWLAYVKLRCVGGDQELGKMIETHARHR
                     IHANASR"
BASE COUNT         1590 a         2037 c         1977 g         1756 t
ORIGIN      
        1 cgtgaaacct gcttttacgg cgcctcgacg atcaactcac gtccgaacgt gaagttaggt
       61 ttcttccgac ccttcatata cttcgccgct gtctgtgaaa gcgacgtgat caagatcggc
      121 agcgcgattg ttgcatcgca gggaacgtaa gcagtatgtg cgccgcgcgc gagcttgccg
      181 tagatcaacc cttcatcaaa cgacactgca ttcgatctgc cgccgggatg cggagcgtcg
      241 gtcgagatct gaatcgcgta cttgtgcccg tgatgatgct ctttctgcat gaacgacgac
      301 attgcggctg tttggataaa actcttcgtg gtcccgccac caagattcag gattccggaa
      361 acacgcgcat tcgccatcac attcatcatg tcgagcacgt cctgaacgag gtcgaacatg
      421 aagttattct tcttctcgaa acgtgcaccg gcgatgccta ccgcaagacc actatcagca
      481 atagccggac aaaagatcgg cacgcgtgcc ttataagccg aagtgagcac gccgtcttcc
      541 gaagcaattt ctgccagctc tcgtcccagc agatgtaaaa actcacgcgg agaataagca
      601 cgcgtttgat cgagttggtt tgtaaatccg ccaatccact ctttcgcttc acgatattct
      661 tcgtcgctgg cgagcaaatc ccacatgcga ctgatctgcg ccgactccaa ctcggcatcg
      721 gtcatacttg gatgagcctg aaagtgataa cgcccgagtg tttcgtgtag atcgtgaaat
      781 agcaccgaac ccgaaaggac cagcacgtca acgaatcggt tcttgattac ataagccagc
      841 aaacggcgca tgccggccgg gatgagcgct ccggaagccg cgacgatgat ggtagtgtta
      901 tcaccaagca tgtcgagcca tatgcgatgc gcctcggcga gttgccttcc tccaaagcct
      961 gttccttcca tcttctcgag caaacccgcc accgaacgat cgcggtccac ctgaacggga
     1021 cgcgtgggag tggtcaggta cttcgatgct ttaggttttc gttgagtgac catgcaggtt
     1081 tgtcaacctc tcggattgaa cgttcctttt tagactttac attttcaata atcgactaat
     1141 aatcagtgcg ccgtcgcgaa tttttcttga cgccattcac tgcgagatag gtataagcct
     1201 cgtattttga ttcgtagaac tgggccaact cggcgccatc ggcctcgctc agctcgccgt
     1261 ctttaacccg ccgggtaatc aaacgacgaa agccgtcgtg caacagactg cgctcgtagc
     1321 gtgcgcgttc cgcagccgcg ccgacagtgg tgccttttat cactttcttg atcaggaaac
     1381 cttcttcgtc gatgtggata tgagcttcgt gcggcgcgcc gaagaggttg tgattatttc
     1441 ccatcgcttc ctgataagcg ccgacgagca ggaatgccag gtagtacggc tcatctggat
     1501 tgaggtcgtg aagttcgagt accggcttga cgtcgtgcag gtccacaaac ttatcgacga
     1561 ttccatcgga gtcgcaggta atgtcgcaga gcgtggtgta ctccgtcggc ggtttgttca
     1621 gatggtgaat cggaacaatc ggaaacagtt gattgatcgc ccagtgatct ggcaccgaac
     1681 gaaacacgga gaagttcgtc agatacttcg cgcaaagcag ccggcgcaat tcgccaaact
     1741 cttccgacac gtatttcgcc tgctgtgcgt atttgtccgc ccgctcgcaa acgtcccaaa
     1801 aaagcacctc gcctttggcg cgatcttcga gcgaaatcaa gccgagattg aacagcgtga
     1861 acaactcttc gcgatgctcg agtgcgtcgt gatagtactc gcgatagttc ttcacggtga
     1921 tcgattcgcg caattcgccg agctcggtta cgacttgcgg atcgtcttcg tcgatctcga
     1981 tcggagtaat gtcttcgacg acggtttcga tctcgtcgag aacattggtg acgaggatcg
     2041 cgtgataagc agccagaaag cgtcctgact cctgaatgat cgtcggatgc ggcacattct
     2101 cgtcgtcgca cacctgtttg attgtgtaga tgacgtcgtt ggcaaactcc tgcgccgtgt
     2161 agttcgcgga agagtcgaaa gcggtttttg atccgtcgta atcgacggcc ataccaccgc
     2221 cgacatccag caactcgatc ggcgctttaa gctggtagac cttcgcgtag actcgggccg
     2281 cttccttcat cgcattcttg atgcgcttga tgtcggtcag ttgcgaaccg atatggaagt
     2341 ggagcagctt cagcatgtcc atgcgatcgg cttcttcgag acgccggatc acttccagaa
     2401 gttcagttgt cgttaaccca aacttcgccg cttcgccacc tgatttttcc cagcggccgg
     2461 agcctttcga atagagcttg acgcgcatgc cgagcatcgg cagcgcgcct tcgtgacgtt
     2521 cgacgacgtt gaggacgtga tccagctcat tcagcttctc gatcacgatc acgactttct
     2581 ttccgacttt cgcgcctgca aaggcaagct cgatgaattc ttcgtccttg aaaccattca
     2641 gaacgagcag gctatcgccc ggttgttcga gcgcgagcgc ggcgtagagt tcggctttcg
     2701 aacccgcttc gagtccgaag tcgtaacggg aaccttcgcg caggtattcc tcgaccacgg
     2761 agcgttgctg gttcaccttc atcgggtaaa cacacatatg cgcaccctgg tattcaaact
     2821 cgcgaatcga gcggctaaag gcgcgctgaa gttttcttac ttgagctgcg acgagctgcg
     2881 gaaagcgcag cagcacgggc gtggtgatgc cacgtttggc aaggtcgtcg ataatctctt
     2941 tgaggtccgc tgagcgtgga tctccctcga cagggcgaac gacaaggttt cccttgcggt
     3001 taacatcaaa gtagccagct ccccagtttt cgattccata agtttcgagc gtttgctcga
     3061 ttgccgtggt caattggcgg tctcctgcct caaaaatcaa acgtgaacga tatgaatgcc
     3121 tttagttgaa acgcagatgt acattttccg aatcgagttg cggagttttt tgtttgggtg
     3181 gaacgcttta gccgcgcaat gtaacagaaa atggccatgc cggtctataa ctatttgcgg
     3241 caaaagcatc cgcagattac gcgtattttg atgcaaattt gtgtaatctg cgaaatctgc
     3301 ggaaagaagt gtctggttta cccttgacat agttttcccg ggtattataa tcacggtttt
     3361 ttacgaatcg tccttcaatg ccgcacggct gtttttctag agtcgttaag cacgccaaaa
     3421 tacttcgcac caaagaccgc gccggggcga agagcacccc caacactccc tttatcttgc
     3481 aggagaacaa atgaacccta agactgtctt gaaatatgct gccgaccagg gcgccaaatt
     3541 cgtttctgtt cgcttcacgg atttgcccgg ctcctggcaa caccttacct tcccgattgc
     3601 cgaactcggc gaagatcagt ttgaagatgg cttcggcttt gacgcctctt ccatccgcgg
     3661 atgggctgcc attcacgagt cagacatgct gctcgttcca gatgctagcc gcttctggat
     3721 ggatccgttt gcggaacagc caaccttgtg tttgatcggc agtgctgtcg acccgataac
     3781 gaaagaagga tacgcattcg atccgcgttc ggttgcgcaa cgcgccgaga gttatctgaa
     3841 gtttaccggc ctggccgaca ccgcttactt tggtccagaa gctgagtttt tcgtgtttga
     3901 ttcagtcaag tttcataacg agcagaattc agccggcttc actatcgact ccgacgaagg
     3961 ccactggaac agcggtcgcg acgcgaacga gtttgccgga tcgaatctcg gctaccgcat
     4021 tcgtccgaag gaaggttacg tgccgtgcgc gccgacagac tcgttgacgg atctgcgctc
     4081 tgagatttcg ctgaccctcg gcaagtgtgg tgtcacggtt gaatgccacc accacgaagt
     4141 tgcgaccgcc ggacagtgcg aaatcgattt tcgtttttcc acgttgctgg gaacggcaga
     4201 caatcttcag atattcaaat atgtagttcg caatactgca caccagtttg gaaaaaccgc
     4261 gacgtttatg ccgaagccgt tgtacggcga caatggttcg ggaatgcact gtcaccagtc
     4321 gttgtggaag agtgagaagc cgctctttgc cggtgacggt tacgcgggac tttcggatat
     4381 ggcgaagttt tatattggtg gattgctgaa acacgccgcc gcaatcgtgg ctttcgccgc
     4441 tcccacgacg aatagctaca agcgtcttgt tcccggtttt gaagcgccgg tgaatctcgc
     4501 gtactcggct cgcaatcgtt cggccgcgat tcgtattccg atgttttcgg cgaatccgaa
     4561 actgaagcga ctggagtttc gtccgcccga tccgtcatgc aatgcttacc tcgcgtttgc
     4621 ggcaatgttg ctcgcgggtt tggatggcgt tcagaatcgc atcgatccgg gcgatccgct
     4681 cgagaaagat atttacgatc tttcgccgga agaactgaag gatgtgccga cactgccggg
     4741 ctcgctcgac gattcgctga aagcgttgga agccgatcac gacttcctgc tcaagggcga
     4801 cgtattcagt ccgcaactga tcgagcgctg gatcagctat aagcgcgatc gtgagatcac
     4861 gccgctgcgc atgcgtccgc acccgctcga gttctcgctg tactacgaca tctaaaacgg
     4921 aaaccagcca cggatgaaca cggatgaata ctgatcagtc acaatcagtg ttcatccgtg
     4981 gcattcatga tcttcccgat cagaacggtc ctcgtctatt tctcgagcgg ttggctaaag
     5041 agcaatcgcg tgctcaccag acacttctca aacaaccagc cctgctcgcg gatgttctcg
     5101 ctctggcggc gtggagtccg ctgctgggga cgactctcga acaaaactcg gaatatcttt
     5161 cctggctcgg tcgcgagcga gcggatccgc gtgttcgaac cgctgacgaa ttcaaagaat
     5221 cactcgcgcg tttcgctctc accaactctt ctctgaatac gcaagtcttg ctcgcgcgtt
     5281 tccggcgtcg cgaattgctg cgtacttatc tgcacgacat tcgtcgctcg caaacgttgg
     5341 ttgagacgat ggaagagttg tcgaacctcg cggacgcgat tctcgatttc gcgctcagcc
     5401 tcgcacgcca ggatctcgac aatcgctacg gcttgccacg gactgtcgac gatcgcggtc
     5461 gcagcgccac ggcagactat tgcgttatcg cgctcggcaa actcggctcg atggagttga
     5521 actacgcttc cgacatcgat cttgttttcc tttactccga cgacggaacg acgtccggtt
     5581 ctggtcaacg cggtgaagtc agcaatcgcg agtacttcgt gaaactgtcg gaaacgatcg
     5641 cgcgtcttgt gggccagtca ggaggcgaag gcgcagctta tcgagtcgat ctgcgtcttc
     5701 gtccgcatgg tcgcgacggc gcgctcgcct gttctcttca ggaaggcgtc ctttactaca
     5761 tgcacactgc gcaggattgg gagcgtcagg cgctgattcg atcgcgagcc gcagtcggct
     5821 cagctgaact cttctcacgt ttcaaagctg ccgtgacacc ggcggttttt cgacccgatg
     5881 tttctatcac aggcgcgctc gcaagcgttc gccttgcgaa acaaaagatc gatcgcaagg
     5941 tcgagaaaaa aagcggtttc aatgtaaagc tcggacgtgg tggcattcgt gagatcgagt
     6001 ttattgcgca ggcgcttcag ttggcacacg gtggacttga cgactggttg cgcgtgccac
     6061 acacgcttgt cagtctcgga cgtctcgccg atcgtggctt catcacgcaa ccggagcgca
     6121 gcgatctgtc tgacgcttat tatttcctgc gctgtttgga gcacaggcta cagatggagc
     6181 acggtctgca aacgcattct gtgcccgaaa gtgaagagac gcagtcactc gtcggacgcc
     6241 gcatgggatt cgccggagcg gacgatttca aagccgcttt aaagacgcac accacaaacg
     6301 tgcgctgcac ttacgatcga ttgttctcag aggctgaaga aactccgaca gtatctgaag
     6361 aattttcacc ggcgcttccg gaagtgggag acaaagacac ggccctggcg ctgggggcgg
     6421 ctcgcgtctt tgtgtcatat atggaccatc gatgggagaa caggccgagg tcgttggcga
     6481 attatttgct gaaactctct gcccgagccc taaatcctca tcgcgccctc gtccagacgt
     6541 ctcgcatcgc ggcttcgctc gaaaagaccg agcaccagtt tttgatttca gaagccaatc
     6601 tcgacgcctt gctgaacctc tgcggcacgt cggattcgtt tgcggaaacg atcgccgcta
     6661 atccggcgtt gattgcttca ctcagtgctc caactgcggc aactctaaga cgcgactacc
     6721 gcgcgattct gcgtaccgca atcgatgccg aacgaagttt tccagcagaa ctggcggcgc
     6781 tgcgtttgca gtgggccgaa ttcatggtcg agatcggcgc gcgcgacgcc cggaacgaac
     6841 tctcaatctt cgaggccaac catttacaga cggacttggc aacggcgagc atgaacgtcg
     6901 cttatctgat cgcgcggcgt gagatggcgc gtcgttacgg ttcgttcagc ggtggaccac
     6961 gcatcgcgtt ttatggactc gggcgactcg gtacgagcgg tgtcgattat ggttctgacc
     7021 tcgacatcct gatcacttac gactctctcg ttccgtcgcc aatcaaaacc ctgacacagg
     7081 atgagaccta ttcgcgtctg gttgagctga tgattgcggc actctcgagt atcacgagag
     7141 aaggttatct ctatcgtgtc gacatgcgtt tgcggccgca tggcaaggat ggacccatcg
     7201 taaccagctc ggagggcttc ctcgactatc taaaagaaca aagcgcgccg tgggagtggc
     7261 tcgcttatgt caagctgcgt tgtgtcggcg gcgatcagga gctcggcaag atgatcgaaa
     7321 cgcacgcgcg ccatcgcatt catgcaaacg cgtcgcgctt
//
LOCUS       DASSFQ010000168         6055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2017439, whole genome shotgun sequence.
ACCESSION   DASSFQ010000168 DASSFQ010000000
VERSION     DASSFQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017439"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VFH96_05815"
     CDS             <1..1196
                     /locus_tag="VFH96_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HET6973521.1"
                     /translation="ENRLPLALPALRKAANIDPDEPDYVFNLGQAAARSEKYKEAADA
                     YERFLIVAPKTDFDRRARIIGLIDFLRYLGRQGLLYVPSGEYRSSVQFEATDNRPVLE
                     VRVNGQKQPLKFVLDTGSGMSVVSEQTAKRLGIKPVAKGGLARAVGGGGKFEIVYGFL
                     DSIEIGDSKISNVPVYIRKFFDSHIPVDGYLGLSVVSKFLTSIDYGTRRMSLVRQQQT
                     EEVEAWTTVRRAEAFQALIPVSPNDGSVEVPLRTTSSGFLSGEVGLDGFDKNVNFIID
                     TAASITVISEKFSQEEQLLDLLQPSKMRVFGAAGVTEDVKLLQLPRLSLGLSRMDKIN
                     AAVLDLEPVNETAGFTQSGILGGNFLRHFRIYFDFARGAMRLEPLNQKPKGVEIMNPE
                     VVVTP"
     gene            1193..1669
                     /locus_tag="VFH96_05820"
     CDS             1193..1669
                     /locus_tag="VFH96_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET6973522.1"
                     /translation="MKQALYLETSVIGAYLDNGEPFRRDLTIRWWEHEMPDYRAVVSP
                     LVSAELSRLKEPHRTSYLKLISHLEQVDLTEEAAILAEGYISRGIFHRKYIADALHVA
                     IASFHKIDYLVTWNFGHLANVRRQARIRLFNTAAGFYVPMIVTPEFLVSEATEQVS"
     gene            1698..1922
                     /locus_tag="VFH96_05825"
     CDS             1698..1922
                     /locus_tag="VFH96_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973523.1"
                     /translation="MYRRPKFLEVLHNIREEMSREADYDVDLFAEMVRSGVQPSHGPQ
                     RVIRGFRTRAPRAETSNGKPVKHRQRKRSA"
     gene            1952..2740
                     /locus_tag="VFH96_05830"
     CDS             1952..2740
                     /locus_tag="VFH96_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3881 family protein"
                     /protein_id="HET6973524.1"
                     /translation="MMNPTFETIGLEVSSEKAFNYLAERAGDQGEPTQLSRRGAVLHG
                     RCWKLGEGLEVWTVLYESGSGDLFYADCRPGFRARYVQKIAPWALTEYDEEGEAVIHG
                     YREGTDCEVLFELQNLTEVGPAGFGEQELHIGLCGLAYRARVLRGKGKTRWTPLEKTA
                     PTRAAHENDWSLSGRVIAFKPLRNPLSGGELFWIHLDLERIKLEVLVNRRALRGELSV
                     GAMLDAEVWLQGHVIDEHALRSRYEGVDFSCTRASFWSGLRRRN"
     gene            2800..3381
                     /locus_tag="VFH96_05835"
     CDS             2800..3381
                     /locus_tag="VFH96_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6973525.1"
                     /translation="MKRVIVVILLALTILLAVYFFNSYWIHRYDALIRTQAAIYRVDP
                     DLVWSIIYEETYFSPWKIGKDGEIGLMQVMPAVGREWAAETGIRDLQSQMLADPQNVL
                     KDPERNIQIGCWYLEKIYSQFRDTPEVETRMVAAYNAGPSRAVEWSRSDPPGSTQSGA
                     EFINRIDIPSTRAYVISIMERYRQLKNAKSASK"
     gene            3378..4700
                     /locus_tag="VFH96_05840"
     CDS             3378..4700
                     /locus_tag="VFH96_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6973526.1"
                     /translation="MTTIYSASWVVPVSAAPIENGAVAVEGQRIAGVGPRAEIVAQFP
                     ESRIEFFGEAIILPGLVNLHTHLELTAMRSYLENEETDFFAWLRKLTVARHELMTADD
                     ILVSATWGACEAVRAGITCVGDASDLGIMSMMALRDVGLRGVVYQESFGPDARHAKEN
                     FEKLQSKADQMRTFQNEVTRVGVSPHAPYTVCGTQLELIAEYAREEGLPLMMHAAESA
                     AEEQFVRDGCGAFAEGLARREIEWKARGVSTIQYLKQVGVLDVQPLLAHCIRVDDRDI
                     ETLRETETKVAHCPKSNAKLGHGRAPFARFLEAGIAVGFGSDSVASNNTCDILEEARF
                     ATLMARAGGGLVPASDALRVATFGRGLSEGALADLAVVSLTGVHQLPSYDPIATLIFA
                     SSGQDVILTVVAGREVYRDGRVSNVDEDRLRARMVEISKKLLAADERG"
     gene            complement(4740..>6055)
                     /locus_tag="VFH96_05845"
     CDS             complement(4740..>6055)
                     /locus_tag="VFH96_05845"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HET6973527.1"
                     /translation="TPKRPEIKTELPGPKGKKIVEADAQFVNPSYPRPSFKLVAERAS
                     GVWVEDVDGNIFLDCNAGVAVCSTGHCHPEVVKAIQEQAAQLIHMCGTDYYYRHMPQL
                     AEKLDAIVPVKSPTRTHFANSGTEAVETALKLAMHKTGREKFIAFFNSFHGRTLGSLS
                     LTSSRAAQRKGFKRQALDVVHVPYPNEFRNPFNPADCGDGGAGQGALNWIEERLFKTT
                     TPPEEVAAIIVEAVQGEGGYVPAPKDFLKGLRRICDEHGILLIVDEVQSGMGRTGKMF
                     ACEHYDVQPDIVCIAKGIGSGLPIGACVARADVMDWKPGAHASTFGGNPVCIAAALKT
                     IELLEGGLVKNSEEVGAYLKEGLVKLQQKHDCIVDVRGFGLMLGVEFENAELRDRIET
                     ACFERGLIILGAGASTIRWSPPLILTKENVDVALEIFNDAITASI"
BASE COUNT         1293 a         1592 c         1806 g         1364 t
ORIGIN      
        1 acgagaaccg tctgccactc gcgctaccgg ctctccgcaa agccgccaac atcgatcctg
       61 atgagcccga ctacgttttt aacctgggcc aggctgcggc gcgcagtgag aaatacaaag
      121 aagctgccga cgcatacgag cgctttctga tcgttgcgcc gaagactgat ttcgaccggc
      181 gtgcccgcat catcgggctc atcgacttcc tccgctatct cggccgccag ggtttacttt
      241 acgtgccgag tggtgaatat cgttccagtg ttcagtttga agcgactgac aatcgtcccg
      301 tgctcgaggt gcgagtcaat gggcagaagc aaccgctgaa gttcgtgctc gacaccggat
      361 cgggcatgtc ggtagtttca gagcagactg caaaacgtct cggcatcaaa ccggtggcga
      421 agggcggtct cgcgcgtgcg gttggtggtg gtggtaagtt tgaaatcgtc tacggctttt
      481 tagactcgat cgagatcggt gattcgaaga tctcgaacgt gccggtctat attcgaaagt
      541 tcttcgacag tcacattccg gttgacgggt atctggggct gtcggtggtc tcgaagtttt
      601 taacttcgat cgattacggc acgagacgca tgtcgctggt gcgacaacag cagaccgaag
      661 aagtagaagc ctggaccaca gtgcgtcgag ccgaagcttt tcaggcgctg attcccgtgt
      721 cgcccaatga tggctcggtt gaagttccct tgcgaacaac gtcgagcggg ttcctgagtg
      781 gcgaagtcgg acttgacggt ttcgataaga acgttaattt tataattgac acggcggcga
      841 gcatcaccgt gatttcggag aagttctcgc aagaggagca gcttctcgat ctattgcagc
      901 cgtcgaagat gcgcgtcttt ggagccgcgg gtgtgaccga agacgtgaag ctgttgcaac
      961 tgccgcgact gagtttgggt ttgagtagaa tggataagat caacgcggcg gtgctcgatc
     1021 tggaaccggt gaacgagact gccgggttta cgcagagcgg catcttaggt ggtaacttcc
     1081 tgcggcactt ccgcatctat tttgactttg ccaggggagc aatgcgtttg gaacctttga
     1141 atcagaaacc gaagggcgta gagattatga atccggaagt ggtagtaact ccatgaagca
     1201 agctctttat ctggagacat ccgtgattgg cgcttacctc gataacggtg agccgttccg
     1261 gcgtgacttg acgatcaggt ggtgggagca cgagatgcct gactatcgcg cggtcgtttc
     1321 accgctcgtg tcggcggagt tgtctcgttt gaaagaaccg catcgcacgt catacttgaa
     1381 gctgatcagc catctcgaac aggttgactt gacagaagag gcggcgattc tcgcagaagg
     1441 gtacatctcg cgcggcatct ttcatcgcaa gtacattgcc gacgcgctgc acgttgcgat
     1501 tgcatcgttt cacaaaatcg attatttggt aacgtggaac ttcggtcatc tcgcgaacgt
     1561 gcgacgccag gcgcgcatcc ggttgttcaa tacggccgcg ggattttatg taccgatgat
     1621 cgtgacaccc gagtttttag tttccgaggc aaccgagcaa gttagttgag ataggaccaa
     1681 taggacttat cggacctatg tatcgtcgcc caaaattttt agaagtgctt cataacattc
     1741 gcgaagagat gtcgcgtgaa gctgactacg atgtcgatct ttttgccgag atggtccgct
     1801 ccggagtgca gccttcgcat ggaccacaac gcgtcatccg aggtttcaga acacgagcac
     1861 ctcgtgctga aaccagcaac ggcaaacccg tcaagcatag acagcgaaag cgcagcgcgt
     1921 aataatggac gcgtaccagt actgataact gatgatgaat ccaacttttg aaacgatcgg
     1981 tttggaagtt tcgagcgaga aggctttcaa ctacctcgcc gagcgcgcag gtgatcaggg
     2041 tgagcccacg cagctcagca gacgcggcgc tgtcttgcac ggtcgctgct ggaaactggg
     2101 agaagggctc gaagtttgga ccgtcctcta cgagtcgggc agtggcgatc tcttctacgc
     2161 tgactgccgt ccgggatttc gcgctcgtta cgttcagaag atcgcgccat gggcgttgac
     2221 ggaatacgac gaagaaggcg aggcagtcat tcacggctat cgtgagggaa ctgactgcga
     2281 agtgctcttc gaattacaga acctgaccga ggttggccca gccggtttcg gcgaacagga
     2341 gctgcacatc ggtctttgcg gtctcgctta tcgcgcgcgt gtgctgcgcg ggaagggaaa
     2401 gacgcgctgg acgccgctcg agaagactgc gccgacgcga gccgcacatg aaaacgactg
     2461 gagtttgtct ggacgcgtga tcgcgttcaa gccgctgcgg aatccgctgt ccggcggcga
     2521 gctcttctgg attcatctcg atctcgagcg catcaagctc gaagtgctcg tcaaccggcg
     2581 cgcgctgcgt ggtgagttga gtgtgggcgc gatgctcgac gcggaagttt ggcttcaggg
     2641 acacgtgatc gacgagcacg ccctgcgttc gcgatacgag ggcgtcgatt tctcctgcac
     2701 gcgcgccagt ttctggagtg gattgaggcg aagaaattaa gccacggatt acacggattt
     2761 acacagtgaa aatccgtgta atccgtggct aatattctaa tgaaacgtgt cattgtcgta
     2821 attctcttag ccctcaccat cctgcttgcc gtctactttt tcaatagcta ctggattcac
     2881 cgctacgacg ccttaatcag aacccaggca gcgatctatc gcgtcgatcc ggatctcgta
     2941 tggagcatca tctacgaaga gacttatttc agtccgtgga agatcggcaa agacggtgag
     3001 atcggtctga tgcaggtgat gccggccgtg ggacgcgagt gggccgcgga aacaggcatt
     3061 cgcgatcttc agagtcagat gctggccgat ccgcaaaacg tcctcaaaga tcccgagcga
     3121 aacatacaga ttggttgctg gtatctcgaa aagatctact cgcagtttcg cgatactcct
     3181 gaagtcgaga cgcggatggt cgcggcttac aacgctggtc cgagtcgcgc ggttgagtgg
     3241 agtcgttccg atccgccggg cagcacgcaa tcgggcgcgg agtttatcaa tcgcatcgac
     3301 attccttcga cgcgcgctta tgtaatctcg atcatggaac gctaccggca gctcaagaac
     3361 gcgaagagcg caagcaaatg acgactatct attcggcgag ttgggttgtt cccgtgtcgg
     3421 cggcgccgat cgagaatggt gcggtcgcgg tggagggcca acgcattgcg ggtgttggac
     3481 cacgcgcgga gattgtcgcg cagtttcccg agtccaggat tgagtttttc ggcgaggcga
     3541 tcattcttcc cggactcgtt aatcttcaca cgcatctcga gctcaccgcg atgcgcagct
     3601 atcttgaaaa cgaggagact gacttctttg cgtggctgcg aaagctgacg gtggcgcgac
     3661 acgagttgat gacggctgat gacattcttg tgtcagccac gtggggtgcg tgtgaagcag
     3721 tgcgcgcggg aatcacgtgt gttggtgatg caagcgatct ggggattatg agcatgatgg
     3781 cgctgcgcga cgttggtttg cgcggcgttg tttaccagga gtcgtttggt ccggatgccc
     3841 gccatgcaaa agaaaacttc gagaagctgc agtcgaaggc ggatcaaatg cgcacattcc
     3901 agaacgaggt cacgagagtt ggtgtttcac cgcacgcgcc gtacacagtt tgtgggacgc
     3961 aactcgagtt gatcgcggaa tacgcgcgcg aagaagggtt gccgttgatg atgcatgcgg
     4021 ctgagtcagc ggcggaagag cagttcgtgc gtgatggctg tggagcgttt gctgaaggac
     4081 tggcgcgccg cgagatcgag tggaaagcgc gcggtgtttc gacgattcag tatttgaagc
     4141 aggttggtgt gcttgatgtt cagccgttgc tggcgcactg cattcgtgtc gacgatcgtg
     4201 atatcgagac attgcgagag actgagacga aggtggcgca ctgtccgaag tcgaatgcga
     4261 agcttgggca cgggcgcgcg ccgtttgcga ggtttttaga ggcggggatc gcggttgggt
     4321 tcggcagtga ttcggtcgcg agcaacaaca cctgcgatat tctcgaagaa gcgcgtttcg
     4381 caacgctcat ggcgcgggcc ggcggcggac tcgtgccggc gagcgatgcg ctgcgagtgg
     4441 cgacgtttgg tcgtgggttg agtgagggcg cgctcgcgga tctcgcggtt gtgtcgttaa
     4501 ccggtgtaca tcaactcccg agctacgatc cgatcgccac gttaatcttt gcttcatctg
     4561 gacaagatgt gatcttgacg gttgttgcag gtcgtgaggt atatcgcgac gggcgcgtaa
     4621 gcaacgtcga tgaagaccgc ctacgcgccc gcatggtaga aatatcaaag aaattattag
     4681 ccgcagatga acgcggataa acgcagatct gcgttcatct gcgtttatcc gcggctaaat
     4741 tagatcgagg cagttattgc gtcgttgaag atctcaagcg cgacgtcgac gttttctttc
     4801 gtcaggatca acggtggtga ccagcggatg gtgctcgccc cggcgccgag gatgatcaag
     4861 ccgcgctcga agcacgccgt ctcaatccga tcgcgcagtt ccgcattctc aaactcaaca
     4921 ccgagcatca atccaaaccc acgcacgtca acgatgcaat cgtgcttctg ctgaagtttc
     4981 accaaacctt ccttcagata cgcaccgact tcttccgagt tcttgacgag cccgccttcg
     5041 agcagctcga tcgtcttcaa ggccgccgca atgcagaccg ggttgccacc aaacgtcgac
     5101 gcatgtgcgc cgggtttcca gtccatcacg tccgcgcgtg cgacacacgc accgatcggc
     5161 aatcccgaac cgatgccttt cgcgatacag acaatgtccg gctgcacatc gtaatgctca
     5221 cacgcaaaca tcttcccggt gcgacccatg cccgactgca cttcatccac gatcagaagg
     5281 attccgtgct catcacagat gcgccgcagt ccttttagaa aatctttcgg cgccggcacg
     5341 taaccacctt ccccttgcac ggcctcgacg atgatcgccg ctacttcttc aggcggcgtc
     5401 gtggttttga acaaacgctc ctcgatccag ttgagggcac cctgtcccgc cccgccatca
     5461 ccacagtcag ccgggttgaa cggattgcga aactcgttcg ggtaaggcac gtgaacaacg
     5521 tcgagcgcct ggcgcttgaa acctttgcgc tgcgcagctc ttgaagatgt cagcgacagt
     5581 gaaccgagtg tgcgtccgtg aaagctattg aagaacgcga tgaacttctc acgaccagtc
     5641 ttgtgcatcg caagcttcaa cgccgtctcg actgcttcag tgccgctgtt tgcgaagtgc
     5701 gttcgcgtcg gcgacttcac cggcacgatc gcatcgagct tctctgcgag ttgcggcatg
     5761 tgacggtagt aataatcagt gccgcacatg tggatcagtt gtgcggcctg ttcctgaatc
     5821 gctttcacga cttccggatg gcaatgacca gtcgagcaga cagcgacgcc ggcgttacag
     5881 tcgagaaaga tgttgccgtc aacgtcttcg acccaaaccc cgctggcgcg ctcggctacg
     5941 agcttgaatg acggccgcgg gtaagaaggg ttaacaaatt gcgcatctgc ttcgacgatc
     6001 tttttgcctt ttggaccggg caactcggtc ttgatctcgg ggcgcttggg tgttg
//
LOCUS       DASSFQ010000169         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1851556, whole genome shotgun sequence.
ACCESSION   DASSFQ010000169 DASSFQ010000000
VERSION     DASSFQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851556"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFH96_05850"
     CDS             <1..396
                     /locus_tag="VFH96_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973528.1"
                     /translation="EGWANTDVARIIAKEYLSDFNKASLGALVLGCTHYPILKKIIAE
                     TVGDQIPLIDSGAATAADVESLLEKSALKHDQLLNLHQDRRLCDDLDHFYVTDAAERF
                     AKVAERFLGSAPSILEAVEIWGHDELRTH"
     gene            374..1129
                     /gene="rph"
                     /locus_tag="VFH96_05855"
     CDS             374..1129
                     /gene="rph"
                     /locus_tag="VFH96_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET6973529.1"
                     /translation="MMSFERTDGRAHDELRPVQITPGFLSYAEGSVLIEMGNTRVICS
                     ASLEDRVPPFLRNSGQGWLTAEYAMLPRSTQTRTSRETGRGGPSGRTHEIQRLIGRSL
                     RAVADMKVLSERTLTIDCDVLQADGGTRTAAITGAYVAFALASHQLLKSGKITRPLTT
                     HQVAAVSVGIVSNTPLLDLKYDEDSKAEVDMNVVCTSDGRFIELQGTAEREPFSRTQM
                     DQLLALATQGIDDLIDLQKAVIAQANGSLASGI"
     gene            1147..1650
                     /locus_tag="VFH96_05860"
     CDS             1147..1650
                     /locus_tag="VFH96_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973530.1"
                     /translation="MKRVISLTLLLLVVAAQIVTAQQAYTHEKVEYTFDLPSPTWRSI
                     LEPDATHENPEFVYGDRLDGYLTIRKEFVDAGTTPSQLAERDAVKLRFLPGFVEGKEE
                     AFNGRLDGITVSYEFVRTGKSMLGRTYYLQADSRTIYALRFTGLRDKLSRIRNQTDLI
                     ARTFKLK"
     gene            1650..2063
                     /locus_tag="VFH96_05865"
     CDS             1650..2063
                     /locus_tag="VFH96_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET6973531.1"
                     /translation="MKLQLSRGLTIKPSPIHGKGCFAAISFKKRHKIAEYTGEKISNA
                     EARRRAHRKMLRICEVNSRWSLDGSRGGNGTHYINHSCSPNAFMQILHDHILFIALRD
                     IQAGEEITIDYQSTLHSDDKRCICGAGNCRGTINR"
     gene            complement(2073..>3407)
                     /locus_tag="VFH96_05870"
     CDS             complement(2073..>3407)
                     /locus_tag="VFH96_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973532.1"
                     /translation="VGQTVGELKQLTVAVPLLHERETAGALLIRPAAQLDDDDKLLLE
                     AVASQFARNLKRESFAKNSARSKSFSYFSQRGSRRKLDALSVLKAITVEQRCEAKALA
                     HITDGVAIAYLDGTVALTNPTILRYAGLTPEHAQQLDLIGLLNTFRTGIFDEPEIAVR
                     RVLQTGIDYEGELDFDQKDQTLKLRISLLRDQQDAATGEPIGIAVYVEDLSQTKDYEK
                     LKSDMISLMSHELRTPLTSINGFAELLTADESIPPQAREFVSIIANESQRMSRMINTF
                     LSVTQLQRSDKQEVLKIPLRLDEVVRETITSLQPVAKKKRIRLIEQPAHRIPPVAADK
                     SLITQAVKNLVHNAIKYSPERTTVTVSTALEAETVRVCVEDRGYGIPAESRERVWDKF
                     YRVVREGQEKDEESTGLGLSFVREVVEQHGGHVELDSLEGRGSKFSFTLPRL"
BASE COUNT          747 a          994 c          941 g          725 t
ORIGIN      
        1 gaaggttggg cgaacacgga cgtcgcacgg atcatagcga aagagtattt gagtgacttt
       61 aataaagcgt cgcttggcgc tctcgtgctt ggttgcactc actacccgat actaaaaaag
      121 atcattgctg aaactgtggg agaccaaatc ccgttgatcg attcaggcgc cgcaaccgca
      181 gcggacgtcg agtctttgct ggaaaagtca gccctgaagc acgatcaact gcttaatctc
      241 catcaggatc gccggctctg tgacgatctc gatcattttt atgtcaccga cgcagccgaa
      301 aggtttgcta aagtagctga aagattctta ggatctgctc cgtcaattct cgaagcagta
      361 gaaatttggg gacatgatga gcttcgaacg cactgacggc cgcgcccatg acgaactgcg
      421 gccggtgcaa ataactcccg gtttcctgtc ttacgccgaa ggctcggttt tgatcgagat
      481 gggaaacaca cgcgtcatct gctccgcgtc actcgaagat cgtgtgccgc cattcctgcg
      541 caattccgga cagggctggc tcaccgccga gtacgcgatg ctgccgcgct caacccaaac
      601 gcgaacctcg cgcgaaaccg gacgtggtgg cccatcaggc cgcacgcacg agattcagcg
      661 actcatcggc agaagcctgc gcgcggtcgc ggacatgaaa gtgctcagcg aacgcacgtt
      721 gacgatcgat tgcgacgtcc tgcaagcaga cggtggcacc cgcaccgcag ctatcaccgg
      781 ggcatacgtc gcgttcgctc tggccagtca tcaactgctc aagagcggaa agatcacacg
      841 gcccttgacg acgcaccagg tcgcggccgt cagcgtcggc atcgtcagca atacaccgtt
      901 gctggatctg aagtatgacg aggattcgaa ggcggaagtc gacatgaacg tggtctgcac
      961 cagcgacggc cggttcatcg aactacaagg cactgccgaa cgcgagccgt tcagtcgcac
     1021 gcagatggat caactcctgg cgctggcaac gcagggaatc gacgatctga tcgacctcca
     1081 aaaggccgta atcgcgcagg caaacggcag tcttgcttcc ggcatctgac ttgggagttt
     1141 ttcggtatga aacgtgttat ttcgctaact ttactcttac tggtggtggc ggcgcagatc
     1201 gtgactgcgc aacaggccta cacgcacgag aaagtcgaat acactttcga cctgccgtca
     1261 cccacgtggc gttccattct ggagccggac gctacgcacg aaaatcccga attcgtttac
     1321 ggcgaccggc tcgacggcta cctcacgatt cgcaaagagt tcgtcgacgc aggaacaacc
     1381 ccctctcaac tggcggaacg ggacgctgtg aaactccgat ttcttcccgg ctttgtcgaa
     1441 ggcaaggaag aggcatttaa cgggcgtctc gacggcatca cggtgtctta cgaattcgtc
     1501 cgcaccggca agtcgatgct cggacgcacc tactacctgc aggccgatag ccgcaccatc
     1561 tacgcgctgc ggttcaccgg cttgcgcgac aagttgagcc gaattcgcaa tcagacagat
     1621 ctaatcgccc gcaccttcaa gctcaaatag tgaagctgca actctcccgc ggactaacaa
     1681 tcaagccttc tcccattcac ggtaaaggct gcttcgccgc catatctttt aaaaagagac
     1741 acaagatcgc cgagtacacc ggtgagaaga tctcgaacgc cgaagcgcga cggcgtgctc
     1801 atcgaaagat gctcaggatt tgtgaagtca atagccgttg gagtctcgac ggcagccgcg
     1861 gcggaaacgg aacgcactac atcaatcatt cctgcagtcc gaatgccttc atgcagatcc
     1921 tgcacgacca cattctcttt atcgccctgc gtgatatcca ggccggcgaa gagatcacga
     1981 tcgattacca gtcaacgctc cactcggacg acaagcggtg catctgcggc gccggcaact
     2041 gccgcggcac gatcaatcgc taaaatccag tatcacagtc gcggcaacgt gaagctgaac
     2101 ttcgagccgc gtccttccag ggaatccagt tccacgtgcc cgccgtgctg ctccaccact
     2161 tcgcgcacaa acgacaatcc cagtccggtc gattcttcgt ctttttcctg accttcgcga
     2221 accacacgat agaacttgtc ccacacacgc tcccgtgatt cagcaggaat gccgtaaccg
     2281 cgatcttcaa cacacacgcg cacggtttcg gcctcgagcg cggtggagac agtcactgtc
     2341 gtgcgctccg gactgtactt gatcgcgttg tggaccaggt tcttcaccgc ctgcgtaatc
     2401 aaactcttgt cggccgcaac cggcggaatg cgatgcgccg gctgttcgat caaacgaatg
     2461 cgctttttct tcgcgaccgg ttgtaacgac gtaatcgttt cgcgcaccac ctcatcgaga
     2521 cgcagcggaa tcttcaacac ttcctgctta tcgctgcgct gcaactgcgt caccgaaagg
     2581 aaggtattga tcatgcgcga cattcgttgc gactcattcg cgatgatcga aacaaactcg
     2641 cgggcttgtg gcggaatact ttcgtcggcg gtcaagagct cggcgaagcc gttgatgctc
     2701 gtcagtggcg tgcgcagctc gtgtgacatc agcgagatca tgtcgctctt aagcttttca
     2761 taatcttttg tctggctgag atcctcgacg tagacagcga tgccgatcgg ttcgccggtg
     2821 gccgcgtcct gctgatcgcg aagcagggag atgcgcagtt tgagcgtctg atctttttgg
     2881 tcaaagtcga gctcgccttc gtagtcgatt ccggtttgca gcacacggcg gacggcgatt
     2941 tccggttcgt cgaaaatgcc ggtgcgaaaa gtgttgagca gaccgatgag gtccaactgc
     3001 tgcgcgtgct cgggcgtgag tccggcgtaa cgaagaatgg ttggattcgt tagcgccact
     3061 gtgccgtcga ggtaggcaat cgcgacaccg tcggtgatgt gcgctagtgc ttttgcttcg
     3121 caacgttgtt cgacggtgat tgccttgagc acgctcaacg catcgagttt gcgccggctg
     3181 ccgcgctgcg agaagtagct gaatgattta ctgcgcgcgg aatttttggc gaagctttcg
     3241 cgtttgagat tgcgtgcgaa ttgcgacgcg accgcttcga gcaacagttt gtcgtcatcg
     3301 tcgagttggg ccgccggccg gatcaggagc gcgcccgcgg tctcgcgttc gtgaagcaac
     3361 gggacagcaa cggttaactg tttcagctcg ccgactgttt gccctac
//
LOCUS       DASSFQ010000170        10583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_77288, whole genome shotgun sequence.
ACCESSION   DASSFQ010000170 DASSFQ010000000
VERSION     DASSFQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10583
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77288"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFH96_05875"
     CDS             <1..460
                     /locus_tag="VFH96_05875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973533.1"
                     /translation="GSGSLHVEVEKRLEAEVASGRVIFTGAVGHERVPGLLDACDILV
                     APHVPLADGSEFFGSPTKIFEYMAMGKGIVASRLGQIGEVLVDGETALLVEPGNVEEL
                     RAAIVRLIEDEGMRRSLGVKAREMAEREHTWTHNARRVLDAYQSLTGLQD"
     gene            475..1278
                     /locus_tag="VFH96_05880"
     CDS             475..1278
                     /locus_tag="VFH96_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6973534.1"
                     /translation="MAEEKLRAREQWGQDPCGAVYDREHELGTRDFFDEVERYRYQEY
                     APWMPRVMEFEKFRGARLLEVGCGMGTDLLQFVRGGAYCTGVDLTPRSVEITRHRFKL
                     YGADANFMLSDGEHLPFRSESFDVVYSNGVLHHTPDTAGSIREVHRVLRPGGVAKIML
                     YHRNSLNYWIEIVLRRGVLGLEFLRGRSAEDIMSRVIEFSDHDARPLVKVYSRKEVRE
                     LFKMFKDVRVEVEQLMRSELRIFSPLVSDSMFDRLCKSIGWNVIITAKK"
     gene            1347..2657
                     /locus_tag="VFH96_05885"
     CDS             1347..2657
                     /locus_tag="VFH96_05885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6973535.1"
                     /translation="MAAISVRVLTLQFMRAHLNDPGWFQVGSYAKFDRQARDILDGRQ
                     KLFWIDDATRTDLAQYPPAFPALVALIYKVTGDRSAYSVQLVIWFADLILSFLLIAGI
                     ALTMFGSRAAVVSAFVVALSPLFAMYAAYPSADVPTTWFVLGGNWLLLLAVKRRSVWL
                     ALAAGVALGIACWLRVNPLYLCVGWAVALLLFMKAAWWLRLKMAAAVLAGTVVLIAPI
                     VIRNYIVFPDFTPTGGTIGANLWEGLGETELGQQHGFIFGDDKMTEVERKRAGWPADM
                     PAEVQFPDGIRRDRERTSESLAFIRQHSVWYAGVMLKRMWGMLKVAGDPVPYTGISGI
                     NVTSKKCLPEHWQGGLLSFGVNLLGMIQSVARYLFLPLAALGIYVGLRRDWRLSFLML
                     VTVLYYLIPGTAAHTEIRYVLPMHGLLIVFSSVSIDYLIRKIRG"
     gene            2664..3401
                     /locus_tag="VFH96_05890"
     CDS             2664..3401
                     /locus_tag="VFH96_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6973536.1"
                     /translation="MKEHTYPIMFRIEQSHWWYTGRRKILTRFVEDICHRVTDRRPRI
                     LDVGCGTGANLLMLSQFGDAEGVDVSEDALAFCRERGLENVKLGAAEKLPYDDGTFDL
                     VTALDVVEHLDDDLAGLREMRRVLRPGGRVLLFVPTFMFLWGLQDDVSNHRRRYRMPE
                     LGRVLEQAGFEIERTTYANITFFMPILAMRKLMRLTGIKAESENNINVSALNGVLARV
                     LGAESYVLRYMNIPFGVSGLCVARTTD"
     gene            3377..4603
                     /locus_tag="VFH96_05895"
     CDS             3377..4603
                     /locus_tag="VFH96_05895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973537.1"
                     /translation="MRRADHGLTRIVYGYVLSNQSIICFGGEDWWYHHPHSKAHLMRK
                     FARAGNKVIFVNSISMGLPSLGHKDLLPRIKRKLGSYSKLARQTDEGITVVSPASLPF
                     FGTAAARSINRRLIGTQIKRLARSRGLTKPILWIAIPTAADMIGTFDESAVVYHVSDK
                     YDANTMDHATDPALIRRLHERAIDAADLVFYSGRKLFTEATRGCERSHLLEQAVEYDH
                     WRRVSDIEVAPAIEKIPRPRLGYFGAVEPWLVDQELIKRAARERPEWQWVFIGNKSRG
                     LEIETLPNTHFLPPVPYAELPAYAAGFDVCVLPWETEQSFTSYGSAIKVREYLATGKP
                     VVISPLPEYEPMRDVLRIARTRDDFIRLVEEALHDTDPADAAKRQAAVASGTWDARAE
                     WVSGLIESVLAADLRG"
     gene            4616..6769
                     /locus_tag="VFH96_05900"
     CDS             4616..6769
                     /locus_tag="VFH96_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HET6973538.1"
                     /translation="MKQVLQNRKTGRPFVGEVPVPALQKGRVLVRTVVSLISAGTERA
                     AVELVSKGLVQEARQRPDLVKAVVAKVKSEGLLNTFASVRDKMAASQSLGYSAAGIVE
                     AVAEDVTEFRAGDRVACAGVGFASHAEVLSVPKNLCVHLPEGVSFESGAYGTLGAIAL
                     QGVRLAEPTLGESVVVIGLGLVGQLTVQLLKANGCRVFGLDLDQSRVALALELGADKA
                     VVSSEAAAKEIETWTRGHGADAVLITAATDSNQPIELAARVSRLKGRVIVVGMTGMDI
                     PRAPFFSRELKLVISMSYGPGRYDPEYEERGHDYPLPYVRWTEKRNIESFLELIADGR
                     LNVERLTTHRFQITEADRAYQLISGELQEPNLGVVLNYDPESEVIRKVSLGAASPVRK
                     SEKSIGLGVIGAGGYVPAMLLPHFKTERVEFRSIATATGVTAHDVGKRFGFAYAVSSA
                     AEVLDDTSVNLVVIGTRNNTHAELARGALERNKHVFCEKPLALNDEQLDSVLEAARSS
                     PAKLMVGFNRRFAPLTQRAKEFFNNVDAPLSVLYRVNAGRIPNEHWMQNAEEGGGRII
                     GEGCHFIDLIQYLVGAPPVSVFAESISARSSKIVDADSVIITLRFADGSHGSVAYLAE
                     GDKGLAKERVEIFGAGRSFVLDDFRRATLYKDGREEQVALKAQDKGQQEQVRVVCASV
                     LQGGAAPIGIDELAATTRTTFRVLDSLRERQPFEI"
     gene            6845..8770
                     /gene="asnB"
                     /locus_tag="VFH96_05905"
     CDS             6845..8770
                     /gene="asnB"
                     /locus_tag="VFH96_05905"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6973539.1"
                     /translation="MCGIAGIVGLEPEQLIAAMLASVEHRGRDDSGTWVSEPIDSSQR
                     RACLGHRRLAIIDTSPAGHEPMFSHDGRYALTFNGEIYNYRELREELRSLGHEFRTDC
                     DAEVLLEAISEWSWDAVGRLNGMFAFAVWDNTERTLTLVRDHVGIKPLYYAFLPAMNA
                     FVFASEIKAILASGLIKAELNPEALHQFLTFLWSPDPNTLFANIKTVPPGHLLHFHND
                     EIKLAQWWDVSFTTIEEGKNEAYWRERVLDTLDRVVKMEMVADVPLGSFLSGGIDSSG
                     IVAMMERHSNGRPISTYTVGIESEDLRYDIIPDDVRWARHVNEQLQTDYHEIMLKPSV
                     ADLLPKLVHHMEEPPIDMAIPSYLISSAARETLTVMLSGMGGDEVFAGYPRQMAMQIA
                     SAFDPVPNMLRRPLMKTVARALPGGRPGRLTAPLRNAKKFATSAALDFEDRYLGYGTY
                     FTNEMKRRLYTDEYRATTSEFDPYATHRGYFARAADAAPLNRLLYVDLKTFLPCLNLM
                     TTDKTSMAANLEVRVPFLNREMIEMAARMPPRLKLRGLKRKYVLKRALESVLPKDVVW
                     RKKAGFGAPIRSWLRGPLRPLIADLLSEETVKRRGIFRPGEVKRILDANFSGREDYNL
                     HVFQLLGLELWHQAFID"
     gene            8915..9304
                     /locus_tag="VFH96_05910"
     CDS             8915..9304
                     /locus_tag="VFH96_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973540.1"
                     /translation="MVNSPEGRKCVLLVEDDPALRRYLEVVLERAGYEVVSAGDGLEA
                     MKFLLGVRIDVIVTDALMPNLDGYELCRFVRNSQHLAHLPIILLSALDPKNTTDESEQ
                     VNAFLAKPVSPEVLLTCIAAFATDSHR"
     gene            complement(9315..10436)
                     /gene="ald"
                     /locus_tag="VFH96_05915"
     CDS             complement(9315..10436)
                     /gene="ald"
                     /locus_tag="VFH96_05915"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET6973541.1"
                     /translation="MIVGLPKEIKDNEYRVGLTPAGVRALKDAGHDIRVETNAGAGSG
                     FEDDLYQRAGATIIGTADEVWATADMIVKVKEPIAPEYPRMREGQLLFTYLHLAPDHA
                     LTQELINRKVTGIAYETITDRRGTLPLLTPMSEVAGRMAIQVGAHYLEKMSGGRGILL
                     GGVPGVPAARVVIIGGGVVGTNAAKIAVGMGAHVTIIDNNLDRLRELDDIFLSKISTL
                     ASSAYMIHDAISQADLIVGAVLVPGAAAPKLVTRGMLKDVPNGAVIVDVAVDQGGCIE
                     TTHPTTHSNPTFYVEGVLHYCVANMPGAVPRTSTFALTNATLPYAMKLANKGFFEAIS
                     KDPGLKEGVNTYAGHCTYEAVASAQGIPYAPLESLINRN"
BASE COUNT         2141 a         2740 c         3305 g         2397 t
ORIGIN      
        1 tgggagtggg agcttgcatg ttgaggttga gaagcgcctc gaggctgagg ttgcaagtgg
       61 acgcgtgatc tttacgggtg cggtgggcca cgagcgtgtg ccgggtttgc tcgatgcttg
      121 cgacatactc gtcgcgccgc atgtgccgct cgctgatggt agtgagttct ttggatcgcc
      181 gacgaagatt tttgagtata tggccatggg gaaagggatt gtggcgagtc gattggggca
      241 gattggcgag gtgcttgttg atggtgagac ggcgttgctg gttgagccgg ggaatgttga
      301 ggagttgaga gcggcgatag tgcggttgat tgaggatgag gggatgcgga ggtctttagg
      361 agttaaggcg cgcgagatgg ctgaaagaga acatacgtgg acgcataatg cgcggcgcgt
      421 gcttgatgca tatcaatcgt tgacaggatt acaggattaa caagattgtc aggattggcc
      481 gaggaaaaac tgagagctcg tgagcagtgg gggcaggatc cgtgtggcgc cgtatacgat
      541 cgcgagcacg agctggggac gcgcgacttt ttcgatgagg tcgagcggta tcgttatcag
      601 gagtatgcgc cgtggatgcc gcgcgtcatg gagtttgaga agtttcgcgg cgcaaggttg
      661 ctcgaagttg gttgtgggat gggaacggat ctattgcagt ttgtgcgtgg tggggcgtat
      721 tgcacaggcg tcgatctcac gccgcgctcg gtcgagatca cgaggcatcg atttaagctc
      781 tatggcgctg atgccaactt catgctctcc gatggcgagc acttgccttt tcgatcggaa
      841 agcttcgacg tggtctactc gaacggtgtt cttcatcaca cgcccgacac agccggatcg
      901 atacgcgaag tgcatcgtgt gcttcgaccg ggtggggtcg cgaagatcat gctctaccat
      961 cgcaactcat tgaactactg gatcgagatc gttctgcggc gcggtgtgct ggggttggag
     1021 tttctgcgtg gtcgatcggc ggaggacatc atgagccgcg tgatcgagtt cagcgatcat
     1081 gatgcgcgcc cattggttaa agtttacagc cgcaaagaag tgcgtgagct gtttaagatg
     1141 ttcaaggatg tgcgagttga ggtggagcag ttgatgcgta gtgagctccg tattttttcg
     1201 ccgctcgtct cggattcgat gtttgatcga ctatgcaaaa gcattggctg gaacgtgatc
     1261 atcacggcaa agaagtagac agccacaaag aggcacacaa agcacaaaaa atcagaagaa
     1321 ccattttgtg ctttgtgtgc ctctttgtgg ctgcgattag cgtgcgcgtc ctcacgctgc
     1381 aattcatgcg cgcgcatctt aacgatcccg gatggtttca agtcggttcg tacgcgaagt
     1441 ttgaccggca ggcgcgcgac attcttgatg gccgccagaa actcttctgg atagacgatg
     1501 cgacacgcac tgacctggcc cagtatccac ccgcatttcc cgcgctcgtt gcgttgatct
     1561 acaaagtcac aggtgatcgc tctgcgtatt cagtgcaact cgtgatctgg tttgcagact
     1621 tgatcctgtc gttccttttg atcgcaggca ttgctttgac tatgtttggc tcgcgggcgg
     1681 ctgttgtgag cgcttttgtc gttgcgctct cgccgttgtt tgcaatgtat gccgcgtatc
     1741 cttcggccga cgtgcctacg acgtggtttg tgctcggcgg caactggctg ctactactcg
     1801 ctgttaagcg caggagtgtt tggctcgcgc tggcggctgg agtggcgttg ggcatcgcgt
     1861 gctggttgcg cgtgaacccg ttgtacctgt gcgtaggttg ggccgtcgcg ctgcttttat
     1921 tcatgaaagc ggcttggtgg ttgcgattaa aaatggccgc ggcagtttta gctgggacag
     1981 ttgttttaat cgcgccgatc gtcatacgca attacatcgt ctttcccgac ttcacgccca
     2041 cgggcggaac aatcggcgcg aatctctggg aaggactcgg cgaaacggag ctcggacagc
     2101 agcacggatt cattttcggc gacgacaaaa tgacggaggt cgagcggaag cgcgccggct
     2161 ggccagcaga catgcctgcc gaggtgcagt ttcctgacgg cattcgacgc gaccgcgaac
     2221 gcacgagcga gtcgctcgca ttcatccggc agcattcggt ttggtatgca ggcgtgatgc
     2281 tgaaacgcat gtggggcatg ttgaaggtcg cgggcgatcc cgtgccttac accggcatca
     2341 gcggcatcaa cgtcacgagc aaaaagtgtc tgccggagca ctggcaaggc ggtttactct
     2401 cgtttggtgt gaacctgctg ggaatgatcc agagcgtcgc gcgatatctc tttctacctt
     2461 tggctgcact tggcatctat gtaggtctgc gacgcgattg gcgactcagt ttcctcatgc
     2521 tcgtcacggt gctttactat ctaattccag gcacggccgc gcacacggag atccgctacg
     2581 ttctgccaat gcacggttta ttaatcgtct tctcatccgt gagtatcgat tacttaatcc
     2641 gtaaaatccg tggctaacga tttatgaagg aacacacgta tccaatcatg ttccgcatcg
     2701 agcagtcgca ctggtggtac accggtcggc gcaagatcct cacacgcttc gtggaagaca
     2761 tttgccaccg cgtcaccgat cgtcgcccgc gcatactcga cgttggttgc ggcacgggcg
     2821 cgaatctttt gatgctttcg cagtttggcg acgcggaggg cgtcgatgtg tccgaagacg
     2881 cgctggcgtt ctgtcgcgag cgtgggctcg aaaacgtgaa gctcggcgcg gctgaaaagc
     2941 ttccgtatga cgatggcacg ttcgatcttg tgacggcgtt ggacgtggtc gagcacctcg
     3001 atgacgatct tgccggactg cgtgagatgc gacgcgtgtt gcggcccggc gggcgcgtgt
     3061 tgttatttgt gccgacgttt atgtttttgt ggggcttgca ggatgatgtg agcaatcacc
     3121 gcaggcgtta tcgcatgccg gagctcgggc gcgtgctcga gcaagccggg tttgaaatcg
     3181 agcgcacgac ttatgcgaac atcacgttct tcatgccgat actggcgatg cgaaagttga
     3241 tgcggctgac ggggatcaaa gccgagagcg agaataatat aaacgtatca gcactgaatg
     3301 gtgtgttagc gcgcgtgctc ggtgcggaga gctatgtgtt gcggtacatg aatattccgt
     3361 ttggtgtttc ggggctgtgc gtcgcgcgga ccacggattg acgcggattg tttacggata
     3421 cgtgctgagt aaccaatcga taatttgctt tggcggtgaa gactggtggt atcaccatcc
     3481 acactccaag gcccacctga tgcgcaagtt tgcgcgcgcg ggcaacaaag tcatcttcgt
     3541 taattcgatc tcgatgggcc tgccgtcgct gggccataaa gatctcttgc cgcgcatcaa
     3601 gcgcaaactc ggcagttact caaaactcgc acgacaaact gacgaaggca tcacggttgt
     3661 ttcccctgcg agcttaccgt tcttcggcac tgcggcagcg cgctcgatca accgacgctt
     3721 gatcggaaca cagatcaaac gcctggcgcg cagtcgcggg ctcaccaaac cgatactctg
     3781 gatcgcgatc cctactgccg ccgacatgat cggcacgttt gacgaatcgg cggtggtcta
     3841 tcacgtgtca gacaagtatg atgcgaacac gatggatcat gctacggatc cggcgttgat
     3901 tcgacggctg cacgagcgtg cgatcgatgc ggctgatctg gtgttctatt cgggtcgaaa
     3961 gctgtttacc gaagcaacgc gcgggtgtga gcgatcgcat ctgctcgaac aggcggttga
     4021 gtacgatcac tggcggcgcg tcagtgacat cgaagttgcg cctgcaatcg agaagattcc
     4081 acggccgcgt ttgggatact ttggcgcggt tgagccgtgg ctcgtcgatc aggaattgat
     4141 caagcgtgcg gcgcgcgagc ggcctgaatg gcagtgggtg ttcatcggta acaagtcgcg
     4201 cgggctcgag attgaaacgt tgccgaacac gcattttttg ccgcccgtgc cgtatgcgga
     4261 gttgccggcg tacgcggcgg gattcgacgt ttgcgtgttg ccgtgggaaa ctgagcagtc
     4321 ttttacgagt tacggatcgg cgatcaaggt gcgcgagtac ctggcgacag gtaagccggt
     4381 ggtgatctcg ccgctgccgg agtatgaacc gatgcgcgac gtgctgcgga tcgcgcgcac
     4441 gcgtgatgat ttcattcggc tggttgaaga ggcgctgcac gataccgatc ctgccgacgc
     4501 agcgaaacga caggccgcag tcgcttcagg aacatgggac gcgcgggctg agtgggttag
     4561 tggattgatc gagagtgttt tggccgcgga tttacgcgga tgaacgcgga tttttatgaa
     4621 gcaagtactt caaaatagaa agacggggag gccgttcgtt ggtgaagtgc cggtgccggc
     4681 gctacagaag ggacgtgtgc tcgtgcgcac tgtggtttcg ttgatcagtg cggggacgga
     4741 gcgtgcggcg gtcgagctcg tcagcaaagg tctggtccag gaggcgcgac agcgacccga
     4801 tctcgtcaaa gcagtcgtcg cgaaagtgaa gagcgaggga ctgctcaaca cgtttgcgtc
     4861 cgtgcgcgac aagatggctg cttcgcaatc gctcggctac agtgcggcgg ggatcgtcga
     4921 ggcagttgcg gaagatgtga cagagtttcg cgctggggat cgggtggcgt gtgcaggtgt
     4981 cggttttgcg tcacacgcgg aagtgttgtc agtgccgaag aacctttgtg tgcacttgcc
     5041 ggagggcgtg agtttcgagt caggagctta cggcacgctc ggcgcgatcg ctttgcaggg
     5101 tgtgcgatta gcagagccaa cgctcggtga gtcagttgtt gtaattggtc tgggtcttgt
     5161 gggccagctc acggtgcaac tgctgaaggc gaatggatgt cgagttttcg gattggacct
     5221 ggaccagtcg cgggtggcgc tagcgctcga gctgggcgcg gacaaggcgg tggtctcgag
     5281 cgaagcggca gcaaaagaga tcgagacgtg gaccagaggt cacggtgctg acgcggtttt
     5341 gatcactgcc gcgactgatt cgaatcagcc gatcgagctg gccgcgcgtg tctcgcgttt
     5401 gaaaggtcgc gtgatcgtgg tcggcatgac cgggatggac atcccgcgcg cgccgttttt
     5461 cagtcgtgag ctcaagctgg ttatttcgat gtcgtatgga ccagggcgtt acgatcctga
     5521 gtacgaagag cgcggtcacg attacccgct gccttacgtg cgttggaccg agaagcgaaa
     5581 catcgagagc tttctcgaac tgattgctga cgggcgtctg aatgttgagc gcctgacgac
     5641 gcaccgcttt cagatcactg aagcggatcg tgcgtaccag ttgatctcgg gagagttgca
     5701 ggagccgaac ctcggcgtcg tgctcaatta cgatcccgaa tcggaagtga ttcgcaaggt
     5761 gtcgctcggc gcggcgtcgc cggtgcggaa gtcggaaaag tcgattgggc tcggcgtgat
     5821 tggcgccggt ggttacgtac cggcgatgtt actgccacac ttcaagaccg agcgtgtcga
     5881 gtttcgctcg attgccactg ctaccggtgt taccgcgcac gacgtcggca agagattcgg
     5941 atttgcgtac gcggtgtcga gtgctgcaga agtgctcgat gatacaagtg tgaatctcgt
     6001 agtgatcggc acgcgcaaca acacgcatgc tgagctcgca cgcggtgcac tcgaacgcaa
     6061 caagcacgtg ttttgcgaaa agccgctggc actcaacgac gagcagctcg actcagtgtt
     6121 ggaagcggcg cgcagttcgc ctgcgaaact gatggtcggg ttcaatcgcc gcttcgcgcc
     6181 gctgacgcaa cgtgcgaaag agttcttcaa taatgtcgac gcgccgctct cggtccttta
     6241 tcgcgtgaac gctggtcgca ttccgaacga acactggatg cagaacgctg aagaaggcgg
     6301 cgggcggatc attggcgagg gttgccactt catcgacttg attcagtatc tggtgggagc
     6361 gccgccggtt tccgtttttg cggaatcgat cagtgcgaga tcgagcaaga ttgttgacgc
     6421 tgattcggtc atcatcacgt tgcgcttcgc ggatggatcg catgggagcg ttgcatatct
     6481 tgctgagggt gacaaagggc tcgcgaagga gcgcgtcgag atatttgggg cgggcagatc
     6541 gttcgtgctc gacgactttc gccgggcgac gctctacaag gatgggcgcg aagaacaggt
     6601 cgcgttgaag gcgcaggaca aaggccagca ggagcaggtg cgcgttgttt gcgcgagcgt
     6661 cttgcaaggc ggagcggcgc cgattggtat cgatgagctg gccgccacca cgcgcacgac
     6721 atttcgggtg cttgattccc tccgcgaacg tcagcccttc gagatttagc cacggataac
     6781 acggatcttc aaaacagatc tacacagatc cgttttcaaa tccgtgtaat ctgtggatgg
     6841 ttttatgtgt ggaattgctg gaattgtagg gctagaaccg gaacagttaa ttgcggcgat
     6901 gctcgcgagt gttgagcatc gagggcgtga tgattcgggg acgtgggttt cggagccgat
     6961 tgattcgtcg cagcgccgcg cgtgtttggg ccatcgacga ctcgcgatca tcgacacgtc
     7021 tcctgccggt cacgagccga tgttctcaca cgacggacgc tatgccctca cgtttaatgg
     7081 cgagatctac aactaccgcg agcttcgcga agaactgcgc tcacttggtc atgagtttcg
     7141 cacagactgc gacgctgaag tgttgctcga agcgatctcc gaatggagtt gggacgcagt
     7201 cggtcgtctc aacggcatgt ttgccttcgc cgtgtgggac aacaccgaac gcaccctcac
     7261 actcgtccgc gatcacgtcg gtatcaagcc gctctactat gctttccttc ccgctatgaa
     7321 tgcatttgtt ttcgcctcag agattaaagc gattctcgcg agcggactga tcaaagccga
     7381 gctcaatccg gaagcgctgc accagtttct gacgtttctc tggtcgcccg atccgaacac
     7441 cctcttcgca aacatcaaaa ctgttccgcc gggtcacctg cttcatttcc acaacgacga
     7501 aatcaaactc gcgcagtggt gggacgtttc cttcacgacg atcgaagaag gaaagaacga
     7561 agcgtattgg cgcgagcgcg tgcttgacac actcgatcgc gtcgtgaaga tggagatggt
     7621 ggctgacgtg ccgctcggct cgtttctttc cggcggcatc gactcgtcag gcatcgtcgc
     7681 gatgatggag cgacacagta atggccgccc gatcagcaca tacacggtcg gcatcgagag
     7741 tgaagatctg cgttacgaca ttattcctga cgacgtgcgt tgggcacgtc acgtcaacga
     7801 gcaactgcag actgactatc acgagatcat gttgaagcct tcggtcgccg atctcctgcc
     7861 gaagctggtc caccacatgg aggagccgcc gatcgacatg gcgattccga gttacctcat
     7921 ctcgagcgcc gcgcgcgaaa cactgaccgt gatgctttca ggcatgggcg gcgacgaagt
     7981 gtttgccgga tatccgcggc agatggcgat gcagatcgcc tccgcattcg atccagtgcc
     8041 gaacatgctg cgacgcccgc ttatgaaaac tgtcgcaagg gcgcttcctg gtggacgccc
     8101 cggacgccta acagcacctc taagaaacgc gaagaagttt gcaactagcg cggccctcga
     8161 ctttgaagat cgctatctcg gttacggaac ctacttcact aacgagatga agcgacggct
     8221 ctacacggat gaataccgcg ccacgacaag cgagttcgat ccttatgcca cgcatcgcgg
     8281 ctatttcgcg cgcgctgcag acgctgctcc gctcaaccgg ctcctgtacg tcgatctaaa
     8341 aacatttctg ccgtgtctca acctgatgac gacagacaag acgtcgatgg ccgcgaatct
     8401 cgaggtgcgc gtgccgtttc tgaatcgcga gatgatcgag atggcggcgc gcatgccacc
     8461 acgtttgaag ctgcgtgggc tgaagcgaaa gtatgtgctc aagcgcgcgc tcgaaagcgt
     8521 tttgccaaag gatgtggtgt ggcggaagaa ggctggattt ggggcgccga tccgttcgtg
     8581 gcttcggggt ccgctgcggc cactcatcgc agacctgctg tcagaggaga cggtgaaacg
     8641 tcgcgggata ttccgtccgg gcgaggtgaa acggatcctg gacgccaact tttccggtcg
     8701 cgaagactat aacctccacg ttttccagct tttagggctt gagctctggc accaagcctt
     8761 catcgattaa atctgcaaaa cattgacaaa ccagcgcgtg gcgggctaac ctgctagggc
     8821 gagccacccc ggctcagaac tcgagagcct cacaggccta agaagtccag ctcactgtat
     8881 gatcacgcag caggaactcg atctagcacc acccatggtt aattcgccgg aaggcagaaa
     8941 gtgtgtcctc ctggtcgagg acgatccggc gttgcgacgt tatctggaag tcgtgctgga
     9001 acgtgcggga tacgaggttg tttccgccgg cgatggtctc gaagcgatga agtttttgtt
     9061 gggtgtgcga atcgacgtga tcgtgaccga tgcgttgatg ccgaacctcg atggttacga
     9121 gttgtgccgc ttcgtgagga acagccagca cctggcccac ctgccgatca tcctgctcag
     9181 cgcgctcgat ccgaagaaca ctactgacga gtctgagcaa gtcaatgcgt ttctcgccaa
     9241 gcccgtgtca cccgaggtgc tgcttacttg catagcagca tttgccacgg attcacacag
     9301 ataaaataca cggattagtt tcgattgatt aggctctcta gtggtgcgta ggggatgcct
     9361 tgggcggagg cgacggcttc gtaggtgcag tggcccgcgt aggtgttgac gccttctttc
     9421 aagccgggat ctttcgagat cgcttcgaag aaacctttgt tggcgagttt catcgcgtaa
     9481 ggcagggttg cgttagtcag cgcgaaggtc gaggtgcgtg gcaccgcgcc cggcatgttg
     9541 gcgacgcagt agtgcagcac gccttcaacg taaaacgtcg gattcgagtg cgtggttgga
     9601 tgtgtcgtct cgatgcagcc gccctggtcg acagcgacat caacgatcac cgcgccattc
     9661 ggaacgtctt tcagcatccc gcgcgtgacg agtttcggtg cggcggcgcc aggcacgagt
     9721 actgctccga cgatcagatc agcttgcgag atagcgtcgt ggatcatgta agcactcgac
     9781 gcgagcgtcg agatcttcga aaggaaaata tcgtcgagtt cgcgcagtcg atcgaggttg
     9841 ttatcgatga tggtgacgtg tgcgcccatg ccgaccgcga tcttcgcagc atttgtaccg
     9901 acaacaccgc cgccgataat caccacgcgc gctgcgggca cacccggaac accaccgagt
     9961 aaaattccgc ggccaccact catcttctcg agatagtggg cgccgacctg gatcgccatg
    10021 cgtcccgcga cttccgacat cggcgtcaac aacggtaatg tcccgcgacg atcggtaatc
    10081 gtctcatagg ctatgcccgt tactttgcga ttaatcaact cttgagtcag cgcgtgatcc
    10141 ggcgcgaggt gcaggtacgt gaagagtagc tggccttcgc gcatgcgtgg atactcgggc
    10201 gcgatcggct cttttacttt cacgatcatg tcagcggtgg cccaaacctc gtcagccgta
    10261 ccgataatcg tcgccccggc gcgttggtag aggtcgtctt caaagccgga acccgcgccg
    10321 gcgttagtct cgaccctgat gtcatgaccg gcatctttga gtgcgcgcac gccggcggga
    10381 gtcagtccga ctcgatattc gttgtcttta atctctttgg gcaggccaac aatcacagtt
    10441 ttattctccg aaacaatcca cagattacac agattacaca gaatctactg aggcgactct
    10501 acttgatcga tctgggcgcg ttgcagtttg ccgctgaagt caacatagat cgacttccac
    10561 tcagaaaaga tgtcgagcac ctg
//
LOCUS       DASSFQ010000171        21142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1851711, whole genome shotgun sequence.
ACCESSION   DASSFQ010000171 DASSFQ010000000
VERSION     DASSFQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21142
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851711"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VFH96_05920"
     CDS             complement(<1..1125)
                     /locus_tag="VFH96_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6973542.1"
                     /translation="MMTNEDQFQLSTYKKMPIAAERGEGVWIYTSDGDKYLDLYGGHA
                     VAGTGHCHPHVVNALCKQAGELLFYSNLVYSSARARAAEKLVSIAPAPLTKTFFCNSG
                     TEANENAMRMARMSTGREKIITFSGGFHGRTADAISATFLGKYRELGKPNVPGHLEAE
                     FGNVESVRALADETVAAIMLEPIQSMAGVRIAQPEFYVALRTLCDERGIVLIYDEVQT
                     GVGRTGQWFFAGSDAGGGVVPDVVTLAKSLGSGVPVGACLVNEAIASHIKENDLGTTF
                     GGGMLAMAAVTATLEAIEQDGMLENVRRVEAYLRERLREVEQVVAVHGLGFLLGIEFN
                     ENAAAVHKRLLEHKIITGTSSDPKVLRLLPPLCLTEEHIDE"
     gene            complement(1159..2196)
                     /gene="argC"
                     /locus_tag="VFH96_05925"
     CDS             complement(1159..2196)
                     /gene="argC"
                     /locus_tag="VFH96_05925"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET6973543.1"
                     /translation="MNTEKTDQIRIGIFGGSGYGGSELLRILLFHPNAEIVFVTANEQ
                     AGKSIGEVHRNLNGLTNLNFIKDPEDLSNLDCVFLALPHGQAMDIVPRLPESIKAIDL
                     SGDFRLRDESVFERHYQQPHTAMHAQAEFVYGLTETNREAIREARLIANPGCFATATL
                     LGLAPLVANGLLNGRVVVDAKTGSSGSGAKAAANTHHPQRMTSFYAYKPFTHQHVPEI
                     EQELAHVGDWTSELVFMTHSLPVARGIFASIYAESKVELAETELREIFADYYRESFFV
                     RLVNGSPDINWVKTTNFCDIGLATRGKQIVVFSAIDNLVKGAAGQAVQNMNLMFGLDE
                     TTGLKLVGTNP"
     gene            complement(2193..3263)
                     /locus_tag="VFH96_05930"
     CDS             complement(2193..3263)
                     /locus_tag="VFH96_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973544.1"
                     /translation="MSTNINVIDVDKIGSATSPLALAKTVALVSSSASTKAGDVVVVR
                     ALTDSATYNMLELPTGRLAKINPGDLLLGVLGRRRALKGFVGDVPASLRSGDELHLLN
                     MGGVMGLCTGHHSSLGDAIKLEVIGLVCDEAGRVQNIADAALPPRTTLGESAPIVMIA
                     GTSMNSGKTYAATELIKQATRAGLRVAAGKLSGVACLRDTLNMADHGAVTTASFLDCG
                     LPSTVDIEDLAPSAKAIVARLNEAHPDLIVIELGDGILGGYSVDTVLADYELLDATAA
                     LVFCASDYVGAWGGIELLRRRGVEVDVIAGSVTDSKMGEDYIRTQFDVPAANARREGG
                     ALFNLVMERVEACKVETVVSHG"
     gene            complement(3260..4456)
                     /locus_tag="VFH96_05935"
     CDS             complement(3260..4456)
                     /locus_tag="VFH96_05935"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET6973545.1"
                     /translation="MKTDIKKIVLAYSGGLDTSVMLRWLKEQYHCEVVCYCADVGQGA
                     ELAGLEEKALATGASKLYVEDLREEFVRDYVWTAVKANAVYEGVYLMGTSLARPVIAK
                     HQIEIARKEGADAVAHGATGKGNDQVRFELTYYALQPDIKVIAPWREWDFKGRMDLIA
                     YAKEHNIPVTATAEKPYSTDANLMHVSYEGGILEDPWAEPPENIFQLTRSPENARAEA
                     QYLEISFVKGEPVAIDGKQLDPVTLLETLNKLGGEHGIGRVDVVENRFVGMKSRGVYE
                     TPGVTILQVAHRALESITLDREVVHLKDSLGVKFAENIYYGFWFAPEFKLMRKMIDET
                     QATVSGDVRLKLFRGNAIVVGRRSPHSLYDEKVATFEADTVYNQRDAEGFIKLNALRL
                     RLGGER"
     gene            complement(4465..4899)
                     /locus_tag="VFH96_05940"
     CDS             complement(4465..4899)
                     /locus_tag="VFH96_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973546.1"
                     /translation="MNKRDRQQKILSLIQAKPVGTQEDLRELLERSGTPATQSSVSRD
                     LEELGVVKHHGHYTLPRTNSFATTRGLVSLDQAGDSLVIARTVPGLASAVAVEIDAAA
                     IEEIVGTIAGEDTIFIAVRDSKSQRAAVKEIWKIFGNTDNTD"
     gene            5053..6405
                     /gene="argH"
                     /locus_tag="VFH96_05945"
     CDS             5053..6405
                     /gene="argH"
                     /locus_tag="VFH96_05945"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET6973547.1"
                     /translation="MSNKLWGGRFEGKIDPGFAEFNKSYGFDRRLFEVDVTASIAYCD
                     ALVKAGVIEAEEGAQIRDALETILKSGADEVNSAEDVHSFVEARLIDLIGDVGRKLHT
                     GRSRNDQVATDFRLWLRGSIDELNETIRDAQTSLLDFAEANRDVVIPGYTHLQRAQPV
                     LLAHWCLAYVEMLKRDRERINEVRRRVNVMPLGSAALAGTSFPINREALATALGFEGV
                     SRNSLDAVSDRDFAVEFLSACSLIMVHLSRLAEDIILYATSEFGFFHLGDAIATGSSL
                     MPQKKNPDSMELVRGKAGRVFGDLMSLLTTLKGLPLAYNKDMQEDKEAVFDALDTTSA
                     SLRVTATVLRNIGIDKERATAAASSGYMNATELADYLVRKGMPFREAHEVVGKIVMRA
                     IESGKELEEMELKQFSDLIAEDVYEALSLERTLGAKNQIGGTAREVVHKALNADRTDL
                     "
     gene            complement(6411..7622)
                     /locus_tag="VFH96_05950"
     CDS             complement(6411..7622)
                     /locus_tag="VFH96_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6973548.1"
                     /translation="MIPGLKKRISVSKSHKLRTAVLPVVIIVLALAFSCSRPGEDNTA
                     PDRTTIEIGYFGDLTGRTFNFGQSAINGVLMATSEVNQSGGINGRKIDIVIEDDKGSP
                     EEAARLTAKLIDQDKVVAIIAGGTSGNSRAAAPKAQASHIPLISPSSTDPAVTQTGNY
                     IFRACFVDTFQGEVMARFAVNTLKAKKAAILTDFNSPYSKGLTDYFKLSFGRLGGTIV
                     SEQTYTQGDADFKGQLSTIRSAEPDVIYIPGYYGEVALIAKQARMIGLTQPLLGGDGW
                     DAPELWQIGGDALNGAYISTHYSADNPSPEIQKFVELYKQRYGNLLPDAHAALAYDAA
                     RILFAAIARTGSTEGDKLRDALSQTKDFNGVTGVISMDANRNAVKPAVVLKLEDLKSI
                     YQETIKPSDQR"
     gene            complement(7585..8289)
                     /locus_tag="VFH96_05955"
     CDS             complement(7585..8289)
                     /locus_tag="VFH96_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973549.1"
                     /translation="MLSLENVSVNYGAIEALTGISLHVEKGEVVTLIGANGAGKTTTL
                     RTITGLLQPRTGKITFEEEEIGGKPTHKLVARGISMSPEGRGVFANLSVRENLQMGAY
                     LIRNKRAVAEDMERAFRMFPRLKEREAQKAGTLSGGEQQMLAMGRALMSKPRLLLLDE
                     PSLGLAPLVVHTIFEAIDEIKSKGTTILLVEQNAHAALGHSDRAYVLETGRIVMEGPS
                     KDLANDPRIKEAYLGE"
     gene            complement(8316..9092)
                     /locus_tag="VFH96_05960"
     CDS             complement(8316..9092)
                     /locus_tag="VFH96_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973550.1"
                     /translation="MSANGSDYLLKTEKTTIRFGGLCAVNELDMAVKKGEIFGLIGPN
                     GAGKTTIFNLLTGVYEPTSGSIYFNGQRINGLRPYQIAARGVSRTFQNIRLFPSMSVL
                     DNVMTACHIHVGQNLLDAVLRTPRFSHDEKKHREYCFELLEIFDLARVCEEGGTSLPY
                     GSQRRLEIVRALATRPQLLLLDEPAAGMNPSEQEDLIHLIKQIRDRFGLTILLVEHNM
                     KVVMGICERIQVVDYGKSIALGLPQEIKNDPKVIEAYLGD"
     gene            complement(9089..10135)
                     /locus_tag="VFH96_05965"
     CDS             complement(9089..10135)
                     /locus_tag="VFH96_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6973551.1"
                     /translation="MPRYLRNLIIGLIVIGVLVGLDFVFQAGIVNPYYTRILMLCGIS
                     ITLAVSLNLINGFTGQFSIGHAGFMAVGAYSSAYFSVNYGMNLANSLGGGKLGWVVAL
                     LLTTIVGAVVAGIAGLAVGIPSLRLRGDYLAIVTLGFGQIIVVFLNNIEAVGGARGYS
                     GIPIVKSFFWIFLIAVLTIVIVYNIVNSAFGRALISIREDELAAEAMGVNTTRYKVMA
                     FVISSAMAGAGGVLLAHFDGYLNPKSFEFIKSFEILIMIILGGLGSIVGSVIGAIVLT
                     ILPESLRGFAEYRMVIYSLLLIILMITRPQGLLGSADAFKGLRKRLRRMTEGPSGTAS
                     SATQSETSVGGERR"
     gene            complement(10193..11161)
                     /locus_tag="VFH96_05970"
     CDS             complement(10193..11161)
                     /locus_tag="VFH96_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003835684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6973552.1"
                     /translation="MSTFLQQLINGISLGAIYSLIALGYTMVYGVLKLINFAHGDVYM
                     VGAFMGYYLANGLGQRGAQMIGMNPDGLIARGLLGGGTLEPSLITALVVMLLAMAICA
                     AIGVIIERLAYRPVRKYSRLTALITAIGVSLLLENGGQVVFGAEPKFFPELFRKRNID
                     LISGASINSADVVVLIIALALMIALQLIVYRTKTGRAMRAVSFNLQSAKLMGINTDRI
                     IAFTFALGSALAAAAGVLVAIRIPRIDPLMGILVGLKAFVAAVLGGIGNIPGAMLGGL
                     LIGITETMVVGYLSPTYKDAVAFAILILILLFRPSGLLGTVAQEKV"
     gene            complement(11180..12340)
                     /locus_tag="VFH96_05975"
     CDS             complement(11180..12340)
                     /locus_tag="VFH96_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6973553.1"
                     /translation="MKRILIASLVIASLLSACQTQGSNSDKVRVGVFMSTTGSTANFG
                     ISSVNGIKMAADEINAAGGINGKQVELLVQDDRSDASEAATIVTKFVTQDQVHAVIGE
                     VASSRSIAAAPIAQNAKIPMLTPSSTNPEVTKKGDFIFRSCFIDPYQGAAIAQFAAKT
                     LGAKTAAIMVDRKNDYSTGLEKVIDQTFTKFGGKIVATQSYQEGDQDFNAQLTSLKGS
                     NPEVIFVPGYYNDVGLIAKQARDKGITVPLIGGDGWDSEQLYKIGGTALNGSYFTNHY
                     SPFDTAPNVVKFVNDYKKRYNSTPDALAATAYDAANIMFDAIKRSKSLSGTDIRDALQ
                     ATNAFPGVTGTVTFNQNRDAVKPIVMIEIKDGGKYEVRERVNVESSAVASNQ"
     gene            12549..13772
                     /locus_tag="VFH96_05980"
     CDS             12549..13772
                     /locus_tag="VFH96_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973554.1"
                     /translation="MISRSRKLACPLFLLVICAQLFAQQPAKPTPSPTPDDDVIRINT
                     NVVQVDATVVDKHGKIVTDLKAEDFEIVEEGKNFRPEYFSFIPLIEQSTEQVEGAATS
                     TTPKPITVDQVKRTFVFLVDNPMIDLSFNNANAFGMSSVSVSMQRRTMRGAVEAERLL
                     TWFIDTQMAANDIAAIVDTELNIGKLSNYTNDRELLKDAIRLIREKAVSNEKAAIRIT
                     SVNGNPNLQPLIQQNLRVIALLSKIIDQTAKIPGRKVVSLVSRGLMYDTRLPGSDIVR
                     ARLDELIAKANRERVTIYTMSPTAIDNLGGVSMSARSRTTPGRISDFNTTQAVQDIDS
                     LQHLASETGGRAIYATNDVRVGFAQVLEENRGYYLLGFNPGAESTGKPHKVKVIVKRP
                     GLKVQARGTAYTRSK"
     gene            13881..15320
                     /locus_tag="VFH96_05985"
     CDS             13881..15320
                     /locus_tag="VFH96_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET6973555.1"
                     /translation="MAKIGGMKLGDMDKEEFRRVGHDLIDWIADYFDHIDELPVLAAI
                     EPGDLKAKLPTSAPVQGEPMESIVADLDRLIVPALTHWSHPSFFAYFATSTSGPGIFG
                     ELLSAAFDNKAMLWRTSPASTELEEVVLDWLRQMMGLDPGMLGIIYDTASVSSLHAIA
                     AAREGVEKRIREEGMSGRSDLPLLRVYVSEQSHSSIEKAVITLGLGQRGLRKIPTDSE
                     FRMDPRALAAAVDEDKRNGYLPFCVVATVGTTSTSSIDPVPEIVPICEEHAIWLHVDA
                     AYAGSAAVVPELRHLLAGCERADSLVTNPHKWLFTPFDLSVLYCRHMDLLKRAFSLVP
                     EYLRTPEQEKVRSGSDYGIQLGRRFRALKLWMVMRYFGQEGLIARIQEHCRLARLFAS
                     WVDESPDWELMAPVPLGLVCFRASQGAADLDSLNESIMHGVNASGRALLSHTRLNDKL
                     TLRLAIGNIRTTEQHVRNVWKLLNEQLKA"
     gene            complement(15386..15784)
                     /locus_tag="VFH96_05990"
     CDS             complement(15386..15784)
                     /locus_tag="VFH96_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973556.1"
                     /translation="MNERSSEFIFCAFCAFCGLSAFVPFCGIKLWQRFDKEIESEPEA
                     PTVVVKGDWKDQRHHKQQHQHVLVVSTDNQQEEEADDQNHDLGGNDVCENRAHKETVL
                     TLEKRHAAWAVMADVERVGDYPGRTTRRTT"
     gene            16095..18743
                     /locus_tag="VFH96_05995"
     CDS             16095..18743
                     /locus_tag="VFH96_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET6973557.1"
                     /translation="MAKQLIVSVNGREKKIAIIEDDKVSEFYIERGEQHQGIVGNIYK
                     GRVMRVLPGMQSAFVDIGLERDAFLYVSDFFDEEEEFERIVIDKSKKGTPDAEHAAAE
                     EVALNRVEREQHIDATQERAEPLLSEIEAPVAAATEEVSEPQDKDRGRRGRRGRRGRR
                     WEKGDEPAPATPPPAPRFEVPDTPFVAGDPSIERIVDEEETALNGEMFKDARLQERLF
                     DQIHAVEFNMEDDFRTAEVGSILTSGFGSESFERVADDEGDAEPIREGSRPPEAHVST
                     FIDDLGSETMPSVGFQRISDDEEEAVGQHIEEKPSKRPRKAAAGGKKRPSKAAATKTA
                     SKKKAASKKSSKKRAASTESDESSEAMTYGDSEARVTESQSRGEFATRRGGRRRRRPG
                     KGKPPSAPAAETDHQVNGQANGEDDEIEAIQFTEPEEIVAAPPPAPAAAPEPSPRQPR
                     GRDQREKRDQRGYRGNQSRRERHQPAITDLLSEGQEILVQIAKEPIAKKGARITSHIA
                     LPGRFLVYMPTVEHVGVSRKIESDNERHRLRKLIQTLRAEEDIPSGGFIVRTAGVGIS
                     EKELREDVRYLVRTWLDIRAAAEKSKAPSLVHRDLDLVQRLLRDQLSDDFESIRVDSE
                     EEYQAIVEFINRIQPRLVKRVKLYTKDQPILEAYGVQVEIDKAIKPRVWLKSGGYLVI
                     NQTEALVAIDVNTGKFVGRGSGRLEDTITRTNLEAVDEIVRQIRLRDLGGIIVLDLID
                     MEERRNRQKVMQALQEALRNDKSPTKVLSFNDFGLVIMTRKRVKQSLERTLCSPCPNC
                     QGAGLIKSPQTICYELLEEARRLAKGINGDKDNKQVTLRINPDVAKALRTSERDVMIE
                     IEDYLGPIDITSDETIHQEQYDFAVT"
     gene            complement(18775..19842)
                     /gene="mqnC"
                     /locus_tag="VFH96_06000"
     CDS             complement(18775..19842)
                     /gene="mqnC"
                     /locus_tag="VFH96_06000"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HET6973558.1"
                     /translation="MTTDECTRLLESHDIARMGVAADEIRARRHPDNIVTYIIDRNIN
                     YTNVCNVVCTFCAFYRRPGAPDTYVRTFDEICAKIDETIALGGTGVLMQGGLHPDFGI
                     EWYEDLLRSLRAKYPGFQLHCFSPPEIHNIHLISKLDYETIMARLKDAGLYSLPGGGA
                     EILDDEVRKRVSTKCTTDEWLDVMRAVHRVGLRSTATMMFGIGDKIEHRVRHLQRIRD
                     VQDETGGFTAFIPWTFQRENTALGRRIKDEPTGVDYLKMLAVSRLFFDNIENFQSSWL
                     TQGLRLGQVALRFGANDMGSIMIEENVVSAAGAHNCADENMLQFLIREAGFIPQQRDI
                     LYKHVNRNTDYTEKTDLALSV"
     gene            complement(19911..20708)
                     /locus_tag="VFH96_06005"
     CDS             complement(19911..20708)
                     /locus_tag="VFH96_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HET6973559.1"
                     /translation="MNAPFRLAASSYLNSAPLIWSFLHGPHEDTVEFVEAVPARCAQL
                     LSRSEVDGALVPVIEYQRIDGGSLVPNVCVGSQKEVLSVVLVSKNKRLENIQSVALDE
                     SSRTSATLVKVIFREFLEREPEWTTRAPNLEEMLEKNDAALIIGDPGMAFPRQGLNVW
                     DMANLWKEHTGLGFVFAMWMVRDEAVSRAREIDFAGARDEGVAHIEEIVQSYQDKIPM
                     RVEELRNYLTENIVFRVDESMERGLRLYFELAFKHGLIERVKPLRLI"
     gene            20747..>21142
                     /locus_tag="VFH96_06010"
     CDS             20747..>21142
                     /locus_tag="VFH96_06010"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6973560.1"
                     /translation="MTYYAPVPIYDISVPISNELPTWPGDPSVQLTVWRSLSSGDEAN
                     VSMLNFGAHTGTHVDAPAHFIEGAAKIESLPLEVLIGAVQVIEVPDDFHAINEEFVLT
                     NCAPETTRVLFKTRNSAFWSESEHVFHTDF"
BASE COUNT         4678 a         5975 c         5899 g         4590 t
ORIGIN      
        1 ctcgtcaatg tgctcctcgg tcagacacag cggcggcaac agcctcagca ccttcggatc
       61 gctcgacgtt ccggtaatga tcttgtgctc gagcagtctc ttatgaacgg ccgccgcatt
      121 ctcgttgaac tcgattccca acaagaatcc gagcccatgc accgccacca cctgctcgac
      181 ttcacgtaaa cgctcgcgta aatacgcctc aacccgccgc acattctcca gcatgccatc
      241 ttgttcaatc gcttcgagcg tcgccgtgac agccgccatc gcgagcatcc cgccgccaaa
      301 cgtcgtaccg agatcgtttt ccttaatgtg tgacgcgatc gcctcattca cgagacacgc
      361 gccgaccgga acaccgctgc ccagcgactt cgcgagggtc acaacgtcag gcacgacacc
      421 tccgcccgcg tcactgccgg caaagaacca ctgccctgtg cgtcccacgc cagtctgtac
      481 ttcgtcgtag atcagcacga tgccacgctc gtcacacagt gtacgtagcg ccacataaaa
      541 ttcgggttgg gcaatgcgca caccagccat cgactggatc ggctcgagca taatcgccgc
      601 gaccgtctcg tctgcgagag cgcgtacgga ttcgacattg ccgaactcgg cttcgaggtg
      661 tccgggcacg tttggcttac cgagctcgcg atacttgccg agaaacgtcg cgctgatcgc
      721 gtcggcagtg cgtccgtgaa agccgccgga gaaggtgatg atcttctcgc ggccagtgga
      781 catgcgcgcc atgcgcatcg cgttctcgtt agcctccgtg ccggagttac agaagaaagt
      841 cttggtcagc ggcgcaggag cgatcgatac gagcttctcc gcagcccgcg cacgtgcact
      901 cgagtagacg aggtttgaat aaaacaacag ctcgccggcc tgtttgcata gggcattgac
      961 gacgtgcgga tggcaatgtc cggtgcccgc gactgcgtga ccgccgtaga ggtcgagata
     1021 cttgtcgccg tcgctcgtgt agatccagac gccttcgccg cgctctgcag cgatgggcat
     1081 tttcttgtac gtggagagct gaaactgatc ttcgtttgtc atcatcagaa aaattagcca
     1141 cggataacac ggatcttgtt acggattggt tccgacaagc ttgaggcccg tagtttcatc
     1201 caggccgaac atcaagttca tgttttgcac ggcctggccg gcggcgccct ttactaaatt
     1261 atcgatcgcg gagaagacca cgatctgttt gccgcgggta gcgagaccta tgtcgcagaa
     1321 gttggtggtc ttcacccagt tgatgtccgg cgagccgttc acgaggcgca cgaagaatga
     1381 ttcgcgataa tagtcagcga agatctcgcg caattctgtt tcggctagct caaccttgct
     1441 ttccgcatag atcgacgcga agatgccgcg cgcgaccggc agcgagtgcg tcataaacac
     1501 aagctcactg gtccaatcgc cgacgtgcgc gagttcctgt tcgatctccg gaacgtgctg
     1561 gtgcgtgaac ggcttgtacg cgtagaacga agtcatgcgc tgcggatggt gcgtgttcgc
     1621 cgcggccttc gcacccgagc ctgacgatcc agtcttcgca tcgacgacaa cgcgaccgtt
     1681 gagcaaaccg ttggcgacga gcggcgcgag ccccaacaac gttgcggtcg caaaacagcc
     1741 gggatttgcg atcagacgcg cctcgcgaat cgcttcgcgg ttggtttcag tcaaaccgta
     1801 aacaaactcc gcctgcgcgt gcatcgccgt gtgcggctgt tggtaatgcc tctcgaacac
     1861 gctctcatcg cgcagccgga aatcgcccga gagatcgatc gctttaatac tttctggcag
     1921 gcgtggaacg atatccatcg cctgcccgtg cgggagcgcg agaaaaacgc agtcgagatt
     1981 gctcagatct tccgggtctt tgatgaagtt cagattcgtg aggccattga gattacgatg
     2041 aacctcaccg atcgacttac cggcctgttc attagccgta acgaaaacta tctcagcatt
     2101 cggatggaac agcagaatac ggagcaactc cgagccgcca taccccgacc cgccaaaaat
     2161 cccaatccga atctgatccg tcttttccgt gttcatccgt ggctaaccac cgtttcaact
     2221 ttgcacgctt ccactctctc cataaccaaa ttaaacagcg caccaccctc gcgccgggca
     2281 ttcgcagcag gcacatcgaa ctgggtcctg atgtaatcct cacccatctt cgaatccgta
     2341 accgacccag cgatcacatc aacctcaaca ccacgccggc gcaacaactc gatgccgccc
     2401 cacgctccaa cataatcaga cgcgcagaac acgagcgccg ccgtcgcgtc gagcaactca
     2461 taatcggcca gcactgtatc caccgaatag ccaccgagaa ttccatcacc caactcaatc
     2521 acgatcaaat cgggatgcgc ttcgttgagt cgcgcgacga tggcctttgc tgacggcgcg
     2581 aggtcctcga tatcgaccgt cgaaggcaaa ccacaatcga gaaaactagc agtcgttacc
     2641 gcgccatggt ctgccatatt caacgtatcg cgcagacaag cgacaccgga aagctttcct
     2701 gccgctactc gcaatcccgc gcgcgtcgcc tgcttgatca attcagttgc agcgtacgtc
     2761 ttgccgctat tcatactcgt gcccgcaatc atcacgatcg gcgcgctttc accgagcgtt
     2821 gtacgcggtg gcaacgccgc atctgcgatg ttttgcacgc gacccgcttc atcgcacacg
     2881 agcccaatca cttccagctt gatcgcatcg ccaagactcg aatgatgtcc ggtgcaaagt
     2941 cccatcacgc cgcccatgtt caggaggtgc agctcatcac cacttcgcag cgacgccggc
     3001 acatcaccca caaagccttt caacgcacga cgacgcccaa gcacgcccag caacagatcg
     3061 ccgggattga tcttcgcgag ccgaccagtc ggcagctcga gcatgttgta cgtcgcgctg
     3121 tcagtcagtg cgcgcacgac gacaacgtcg cccgcttttg ttgacgcaga gctggaaaca
     3181 agcgcgaccg ttttcgcgag tgcgagcggt gatgttgccg aaccgatttt gtcgacgtcg
     3241 atgacgttga tatttgtgct catcgttcac ctcccaatct gagtcgcagc gcattcagct
     3301 tgatgaaacc ttctgcatcg cgctggttgt aaaccgtatc agcctcgaac gtggccacct
     3361 tctcgtcgta aagcgaatgc ggcgagcgac gtccaaccac gatcgcgttt ccacgaaaga
     3421 gctttagacg aacatcgccg ctcacagttg cctgagtctc gtcgatcatt tttcgcatca
     3481 gcttgaactc aggcgcgaac cagaaaccgt aataaatatt ttcggcgaac ttcacgccga
     3541 gcgaatcctt caaatgcacc acttcacgat cgagtgtgat cgactcgagg gcgcgatgtg
     3601 cgacttgcag aatcgtgacg cccggcgtct cgtaaactcc gcgcgacttc atgccgacaa
     3661 aacgattctc aaccacatcg acgcgcccga tgccgtgctc gccaccgagc ttgttcaatg
     3721 tttcgagcag cgtcacggga tccaactgct tgccgtcgat cgcaaccggc tcacctttga
     3781 cgaatgagat ctcgaggtac tgtgcttcgg cgcgcgcgtt ctcaggcgag cgcgtgagct
     3841 gaaaaatatt ctcgggcggc tcggcccatg gatcttcgag aattccgcct tcgtaagaga
     3901 cgtgcatgag attcgcgtca gtcgagtaag gcttctcggc cgtcgcggtt acgggaatgt
     3961 tgtgctcctt cgcatacgcg atcaggtcca tgcgaccttt gaagtcccac tcgcgccacg
     4021 gggcaatgac tttgatgtcc ggctgcagcg cgtaatacgt cagctcgaag cgcacctggt
     4081 cgttgccttt gccggttgcg ccgtgtgcga cagcgtccgc gccttctttg cgcgcgatct
     4141 cgatctggtg tttcgcgatc acaggccggg cgagggaagt gcccatcaga tacacacctt
     4201 cgtaaaccgc gttcgctttg accgcggtcc aaacgtagtc gcgcacaaac tcctcgcgga
     4261 gatcttcgac atagagtttc gaagcgccgg tcgcaagcgc cttctcttcg agccctgcga
     4321 gctccgcgcc ctggcccacg tcagcgcagt aacacacgac ttcgcagtga tactgttcct
     4381 tcagccagcg gagcatgact gacgtgtcga gcccccccga atatgccagc acaattttct
     4441 taatatccgt cttcatccgt gtcttcaatc cgtgttatct gtgtttccga aaattttcca
     4501 aatctctttc actgccgcgc gctgtgactt cgagtcgcgc acggcgataa agatcgtgtc
     4561 ttcaccggcg atcgtgccga cgatctcctc aatcgcggcg gcgtcgatct cgacagcgac
     4621 tgctgatgca aggcccggaa cagtgcgcgc gattaccaga ctgtcgcccg cctggtctaa
     4681 actcacgagg ccgcgcgtgg ttgcgaagct gttcgtgcgc ggcaacgtgt aatggccgtg
     4741 atgtttgacg acgccgagct cttcgagatc gcgcgagacg ctcgactgcg tggcgggcgt
     4801 gcccgagcgc tcgagcaact cgcgcaagtc ttcctgcgtg ccaaccggct tggcttggat
     4861 caggctcaaa atcttctgtt gtcggtcccg tttattcatt ctacttgcat aaacactgca
     4921 tagacgcata aactatcacg ggcgttggca gatcgtcaaa cttagacagg atttacagaa
     4981 tttttcagga tgaacatata aatcctgttc gaatcttgta gatcctgtct aagacttcgt
     5041 tgaggagctg gtatgtcgaa caaactttgg ggcggtagat ttgaagggaa gatcgatccg
     5101 ggctttgccg agttcaataa gtcctatggc ttcgatcgca ggctcttcga ggtggacgtc
     5161 actgccagca ttgcttattg cgacgcgttg gtgaaagcgg gcgtcatcga agccgaggaa
     5221 ggggctcaga tccgcgacgc tctcgaaacg atcctcaaga gcggcgctga tgaagttaat
     5281 tctgccgaag acgtgcattc attcgtcgaa gcacgactga tcgatctgat tggcgacgtc
     5341 ggccgcaagc tgcacaccgg acgcagccgc aacgatcaag tcgcgacaga ttttcgactc
     5401 tggctgcgcg gatcaattga tgagctcaat gaaaccattc gcgacgcaca aacttcactg
     5461 ctcgatttcg ctgaagcgaa tcgcgacgtt gtcattcccg gatacacgca cttgcaacgt
     5521 gcacaaccag tcttactggc tcactggtgt ttagcttacg tcgagatgct taaacgcgat
     5581 cgggagcgca ttaacgaggt gcgccgtcgc gtgaacgtga tgccgttggg ctctgcggcg
     5641 ctggcgggca cctcgtttcc gattaatcgc gaagcactgg caacggcgct cggtttcgaa
     5701 ggagtttcgc gcaacagtct cgacgcggtg agcgatcgtg attttgcggt ggagtttttg
     5761 agtgcgtgct cgttgatcat ggtccacctg tcgcgactcg cagaagacat cattctttac
     5821 gcaacgagcg agttcgggtt tttccatctc ggcgatgcga tcgcgacggg ctcgagcctg
     5881 atgccgcaga agaagaatcc cgactcgatg gagctcgtgc gtggtaaggc cggccgcgtg
     5941 tttggcgatc tgatgtcgtt gctcacaaca ctgaaaggac tgccgctcgc atacaacaaa
     6001 gacatgcagg aagacaaaga ggcggtgttt gatgcgttgg atacgacctc cgcgagtctg
     6061 cgtgtgacgg ctacggtttt gaggaatatc gggatcgata aagagcgagc gactgcggca
     6121 gcgtcgagtg gttacatgaa cgcgactgag cttgcagatt atctcgtgcg gaagggaatg
     6181 ccgttccgtg aggcgcacga ggttgtcggg aagatcgtga tgcgtgcgat tgagtcaggg
     6241 aaagagttgg aggaaatgga gctcaagcag ttctcggatt tgattgcaga agatgtttat
     6301 gaggcgttat cgcttgagag gacgttgggt gcgaagaatc aaattggggg gacggcgcgt
     6361 gaggtggttc ataaggcgtt gaacgcggat cggacggatt tataaaacga tcagcgctga
     6421 tcagagggct tgatggtttc ctggtagatg gatttgagat cttcgagctt caagacgacc
     6481 gcgggtttta cggcgttgcg atttgcatcc atgctgatca cgccggtgac gccgttgaaa
     6541 tcttttgttt gtgagagggc gtcgcggagt ttgtcacctt cggtcgagcc ggtgcgtgcg
     6601 attgcggcaa acaagattcg cgcggcgtcg taagcgaggg ccgcgtgagc gtcaggcaac
     6661 aagttcccgt aacgctgctt gtacaattcc acaaactttt gaatctccgg cgacggatta
     6721 tcagccgagt agtgcgttga gatgtacgcg ccgttcaacg catcgccacc gatttgccag
     6781 agctccggtg cgtcccagcc atcgccaccg agcagcggct gcgtcaaccc gatcatgcgt
     6841 gcttgtttgg cgatgagggc aacctcgccg taataacccg gaatgtagat tacgtcaggc
     6901 tcagcggagc gaatcgtgct cagttgtcct ttgaaatccg catcaccctg cgtgtacgtc
     6961 tgctcactca cgatcgtccc accgagccgt ccaaagctga gcttgaaata atcggtcagt
     7021 cctttgctgt aaggtgagtt gaagtctgta agaatcgcgg ccttcttcgc tttaagggtg
     7081 ttgacggcaa acctcgccat cacttcgccc tgaaacgtgt cgacgaagca cgcgcggaaa
     7141 atgtagttgc cggtctgcgt caccgcggga tcagttgacg acggcgagat caacggaatg
     7201 tgcgacgcct gcgctttcgg agcagcagcg cgactgttgc ctgacgtacc gccggcaatg
     7261 atcgcgacga ctttatcctg atcgatgagt ttggcggtga gtcgcgctgc ttcttccgga
     7321 ctgcccttgt cgtcttcgat cacgatatcg atcttccggc cgttgatgcc gccagactga
     7381 ttgacttcag acgtggccat cagtacgccg ttgatcgctg actgtccgaa gttgaatgtt
     7441 cgtccagtga gatcgccgaa gtaaccgatc tcgatcgtgg tgcggtctgg tgcggtgttg
     7501 tcttcgcccg gtctgctaca cgagaaggcg agtgccagca cgatgatgac gacgggaagt
     7561 actgcggttc gcagcttgtg agatttactc accgagatac gcttctttaa tcctgggatc
     7621 attggcgagg tcttttgatg gaccttccat gactattctt ccggtttcga gcacgtaggc
     7681 gcgatcggaa tgtccgagcg cggcgtgcgc gttttgttcg accaggagaa tcgtagtccc
     7741 tttgcttttg atttcgtcga tagcttcgaa gatcgtatgg accacgagcg gcgcgaggcc
     7801 gagtgacggc tcgtcgagca gcagcaatcg tggcttcgac atcagcgcgc gtcccatggc
     7861 gagcatctgt tgttcgccgc ccgaaagcgt tccggctttc tgtgcttcgc gttctttgag
     7921 acgcggaaac attctaaagg cgcgctccat gtcttcggcg acagcgcgct tgttccggat
     7981 caggtacgcg cccatctgaa gattctcgcg cacggaaagg tttgcgaaca cgccgcgacc
     8041 ttcaggcgac atcgagatac cgcgcgcgac gagtttgtgc gttggcttgc cgccgatctc
     8101 ttcttcttca aaagtgattt tgccggtgcg tggctggagc aggccggtga tggtgcgcag
     8161 cgtggtggtt ttgccggcgc cattcgcgcc gatgagcgtg actacttcgc ctttttcgac
     8221 gtggagcgag atgccggtca aggcctcgat cgcgccatag ttaacggaaa cgttttctaa
     8281 tgaaagcata ggacccatag gacgtatacg acctattaat ctcctaaata cgcctcgatc
     8341 actttcggat cgttcttgat ctcctgcggc aggccgagcg ctatggattt gccgtagtcc
     8401 accacctgga tgcgctcgca aattcccatc actaccttca tgttgtgttc gacgagcagg
     8461 atcgtcagac cgaagcggtc ccggatctgt ttgatcaggt gaattagatc ttcctgctcg
     8521 gaaggattca tgcctgcggc cggctcatcg agcaacaaca actgcggtcg cgtcgcgagc
     8581 gcgcgcacga tctcgagtcg tcgttgtgag ccgtacggca aactcgtgcc gccttcctca
     8641 cacacacgcg ccaggtcgaa gatctcgagc agctcgaaac agtattcacg atgcttcttc
     8701 tcgtcgtgac taaagcgcgg cgtgcgcaag acggcgtcca gcagattttg gccgacgtga
     8761 atgtgacagg ccgtcatgac gttatccagc actgacatgc tcggaaacag tcgaatgttc
     8821 tggaacgtgc gcgagacgcc gcgcgctgcg atctgataag gacgcagacc attaatgcgt
     8881 tggccgttga aatagatcga gccggacgtg ggctcgtaaa cgccggtgag cagattgaag
     8941 atcgtcgtct tgccggcgcc gtttggaccg atgagaccga agatctcgcc tttcttcacg
     9001 gccatgtcga gttcgttgac ggcacagagc ccgccgaaac ggatcgtcgt cttttcagtt
     9061 tttaacaggt agtcggaacc gttcgcgctc aacgcctctc tcctccaact gaggtttcac
     9121 tctgcgtggc cgagctggca gtcccactcg ggccttcggt catcctgcgc aaacgtttgc
     9181 ggagtccttt aaatgcatcg gcggagccga gcagtccctg tggtcgcgtg atcatcagga
     9241 tgatcagcaa gagggaatag atcaccatgc gatactcagc aaagcctcgc aaactctccg
     9301 gcaggatcgt caacacgatc gcgccgatca ccgatccgac gatcgatccg agaccgccga
     9361 gaatgatcat gatcaggatc tcgaacgact tgatgaactc aaacgacttc ggattgagat
     9421 aaccatcgaa gtgagcgagc agcacaccac cggcgcccgc cattgcggag gagatcacaa
     9481 acgccatgac tttgtagcgc gtcgtgttga cgcccatcgc ttccgcggcg agctcgtctt
     9541 cacgaatcga gatgagcgcg cgtccgaaag ctgagttgac gatgttgtaa acaatcacga
     9601 tcgtcagtac cgcgatcaga aagatccaga agaagctctt tacgatcgga atgcccgaat
     9661 acccgcgcgc gccgccgaca gcctcgatgt tgttgagaaa cacgacgatg atctggccga
     9721 acccaagcgt gacgatcgcg agatagtcgc cgcgcaaacg caacgacgga ataccaaccg
     9781 cgagacccgc gatcccggca acaactgcac cgacgatggt cgtcagtaaa agagctacga
     9841 cccagccgag cttcccgccg ccaagcgagt ttgcgaggtt cattccgtag ttgacggaaa
     9901 aataggcaga cgaataagcg ccgacggcca tgaagcccgc gtggccaatc gaaaactgtc
     9961 cggtgaaacc gttaatcagg ttgagtgaaa cggcgagcgt gatcgagatg ccgcacagca
    10021 tcaggatacg cgtgtagtaa ggattgacga tgcccgcctg gaagacgaaa tccaagccca
    10081 cgaggacgcc gatcacgatc aggccgatga tgagattgcg aagataccgg ggcatatcaa
    10141 ctatccaccg attacgcaga ttacacaaac tctgtgaaat ctgtggatgg gtctacactt
    10201 tctcctgagc gaccgtaccg agaagcccgg aaggccggaa aagtagtatc aagatcagta
    10261 tcgcaaatgc gactgcatct ttgtaagtgg gggacagata tccgacgacc atcgtctcgg
    10321 taattccgat cagcaaaccg ccgagcattg cgccggggat attgccgatg ccgccaagca
    10381 cggcagcgac gaaagctttc aacccgacca gaatacccat caacgggtcg atacgcggaa
    10441 tacgaatcgc aaccagcacg cccgcggccg ccgcgagggc cgagccgagc gcgaaggtaa
    10501 atgcgatgat gcgatccgtg ttgataccca tcaacttcgc cgactgcaag ttgaacgaca
    10561 ccgcacgcat cgcacggcca gtcttcgtcc ggtacacaat caactgcaac gcgatcatca
    10621 acgcgagggc aatgatcaaa acgaccacat ccgcgctgtt gatactcgcg ccgctgataa
    10681 gatcgatatt gcgtttgcgg aagagctccg ggaagaactt cggctccgcg ccgaacacta
    10741 cctgaccacc gttttccagc aagagggaaa caccgatcgc cgtgatcagc gccgtcagtc
    10801 gcgagtactt gcgcaccggt cgatacgcga gacgctcgat tatcacaccg atcgccgcac
    10861 agatcgccat cgccagtaac atcaccacga gggccgtgat cagcgacggc tcaagcgtgc
    10921 cgccaccgag cagtccgcgc gcaatgagtc catccgggtt catgccgatc atctgtgcgc
    10981 cgcgttggcc gagcccgtta gcgagatagt agcccatgaa cgcgccgacc atgtacacat
    11041 cgccgtgcgc gaagttgatc agtttcagta ccccgtaaac catcgtgtag ccgagcgcaa
    11101 tcagcgagta gattgcgccc agcgagatac cgttaattag ttgttgaagg aacgtgctca
    11161 tgatcgaggg ttgtagcgat cactgattag acgccaccgc tgagctttca acgttgacgc
    11221 gttcccttac ttcatatttg ccgccgtctt tgatctcgat catgacgatt ggcttaactg
    11281 cgtcgcggtt ctggttgaag gtgacagtgc cggtcactcc gggaaacgcg ttcgtggcct
    11341 ggagcgcgtc gcgaatgtct gttccactaa gcgacttgga acgcttgatg gcgtcaaaca
    11401 tgatattcgc ggcgtcgtaa gcggttgctg cgagcgcgtc gggcgtgctg ttgtagcgtt
    11461 ttttgtagtc gttaacaaac ttgaccacgt tcggcgcggt gtcgaaaggc gagtagtggt
    11521 tggtgaaata agagccgttc agggccgtgc cgccgatctt gtaaagctgt tctgaatccc
    11581 agccgtcgcc gccaatcagc ggcaccgtaa tacctttgtc acgcgcctgc ttggcgatca
    11641 agccgacgtc gttgtaataa cccggcacga agatgacttc aggattcgag cctttcagtg
    11701 acgttagctg cgcgttgaag tcctggtcgc cttcctggta cgactgggtc gcgacgatct
    11761 tgccgccgaa tttagtgaat gtctggtcga ttactttctc gagtccggtt gaatagtcgt
    11821 tcttccggtc gaccatgatc gcggcggtct tggcgcccag tgtcttggcg gcaaactgcg
    11881 cgatggcggc gccttgatac ggatcgatga agcaactgcg gaagatgaag tcgcccttct
    11941 tcgtgacttc gggattagtc gaagacggcg tcaacatcgg aatcttcgca ttctgtgcga
    12001 ttggtgccgc ggcgatcgag cgggagctcg cgacctcgcc gatgacggcg tgaacctggt
    12061 cctgcgtcac aaacttcgtc acgatggtgg ctgcttctga cgcgtcagag cgatcgtcct
    12121 gcaccagcag ttcgacctgt ttaccattga tgccgccggc ggcgttgatc tcatccgcgg
    12181 ccatcttgat accgttgacc gaagagatgc cgaagtttgc tgtggagccg gtggtcgaca
    12241 tgaagacgcc gacccggact ttgtcgctgt tactgccttg cgtttggcaa gcgctcagca
    12301 aactcgctat aactaatgaa gctatcagaa tgcgtttcat acaaatcctc acagggctag
    12361 agtttctgac gaacttttaa gtccgaaccg gaaccgggtg actgttcttg tctttaatct
    12421 cgcagaaggg agaccgaagc ttttgatatt ctgcagtaat cgcgctgaat aatagctttc
    12481 gagcgcttgt taagcaaatc ttgtaagcta tctgactgtt accacaactc ttaaacggat
    12541 aataaacgat gatttctcgc tcccgaaagc tcgcttgccc gttgtttctg ctggttattt
    12601 gcgcgcagtt attcgcacag cagcctgcca agcccacgcc ttcgccaact cccgacgacg
    12661 acgtgatccg cattaacacg aatgtggtgc aggttgatgc gaccgtggtc gataagcacg
    12721 gaaaaatcgt gaccgatctg aaggccgagg attttgagat cgtcgaggaa gggaaaaatt
    12781 tcaggccgga gtatttctcg tttattcccc tcattgagca aagcacggaa caggtcgaag
    12841 gcgccgcgac atccacaacc cctaaaccga tcacggtcga ccaggtaaag cgaacctttg
    12901 tttttctggt agacaatccg atgatcgacc tcagcttcaa caacgccaat gcgtttggaa
    12961 tgtcgagcgt gagtgtcagt atgcagcgcc ggaccatgcg tggagcggtt gaagcggaga
    13021 gactactgac gtggtttatc gatacgcaga tggcggcgaa cgatatcgcg gccatcgtcg
    13081 ataccgagtt gaatatcggt aagttgagca actacacaaa cgatcgcgag ctgctgaagg
    13141 atgcgattcg gctgattcgt gaaaaggcag ttagtaatga aaaggccgcg atcaggatca
    13201 cgagtgtgaa tggcaatccg aatctgcaac cattgataca gcaaaatctg cgcgtgatcg
    13261 cgctgttgag taagatcatc gatcaaacag cgaagattcc gggtcgaaaa gtagtcagtc
    13321 tggtttcgcg tggtctcatg tacgacacac gattgccggg ttctgacatt gtgcgcgcgc
    13381 ggcttgacga gctgatcgcg aaggcgaatc gagagcgcgt aacgatctac acgatgagtc
    13441 cgaccgcgat tgataatctt ggtggagttt caatgagtgc gaggagccgg acgactccgg
    13501 gccgaataag cgacttcaat acgacgcagg cggtgcagga tattgatagt ctgcagcatc
    13561 tcgcgagcga gaccggcggc cgggccatct acgctaccaa cgatgtgcgt gtcggtttcg
    13621 cgcaggtgtt ggaagagaat cgtggttatt acctgctggg cttcaatccc ggagctgagt
    13681 cgacgggcaa accacataag gtgaaggtga tcgtgaagcg tccgggtctg aaagtgcagg
    13741 cacgcggcac ggcgtacaca agatcaaagt aggccacaaa aggcacaaaa gaacaaaaag
    13801 cacaaaaaag cacttgctgt tctttcattc ctctctctgg tttttctttg catttctttt
    13861 ttgtgccttt tgtgcctttt gtggctaaga ttggcggcat gaaactgggc gacatggaca
    13921 aagaagagtt tcgtcgtgtg ggccacgatc tgatcgactg gatcgcggat tattttgacc
    13981 acatcgacga gctccctgtg ctcgctgcga tcgaaccggg cgacttaaaa gcgaagctac
    14041 ccaccagcgc gcccgtgcag ggcgaaccga tggaatcgat cgtggcggat ctcgatcgat
    14101 tgatcgtgcc cgcgttgaca cactggagtc atccctcatt cttcgcctac ttcgccacgt
    14161 caacttcagg gcccggcatc ttcggcgagc ttctgagcgc ggccttcgat aacaaagcga
    14221 tgctctggcg cacttcgccg gcctcaactg agctcgagga agtcgtactc gattggttgc
    14281 gacagatgat gggcctcgat ccgggaatgc ttgggatcat ttacgacacg gcgtcagtat
    14341 cgagcttgca cgcgattgcg gcggcgcgcg aaggagtcga gaagcgcatt cgcgaagagg
    14401 gaatgagtgg tcgatctgat ctgccgctgt tgcgtgttta cgtgtctgaa cagtctcact
    14461 catcgatcga gaaagcagtg atcacgctcg gtctgggcca acgcggcttg cgcaagattc
    14521 ctaccgactc cgaattccgc atggatccgc gcgcgctcgc tgctgcagtg gatgaagaca
    14581 agcggaatgg ttatctgccg ttctgcgtcg tggcaacagt tggtacgacg tcgacttcca
    14641 gcatcgatcc ggtgccggag atcgttccga tatgcgaaga gcacgcgata tggctacacg
    14701 ttgatgcggc gtatgcgggg tcggcggcag tcgttccgga gctgcggcac cttctggcgg
    14761 gttgtgagcg cgcggattct ttagtgacta acccgcacaa gtggctcttc acgccgtttg
    14821 atctttcggt gctttactgt cgtcacatgg acctccttaa gcgcgccttc tcgctcgtgc
    14881 ctgagtattt gcgcacgccc gaacaggaga aggtgcgcag tgggtcggat tatgggattc
    14941 aactcggacg acgcttccgg gcgctgaagt tgtggatggt gatgcgctac ttcggacagg
    15001 agggtctgat cgcccgtata caggaacact gtcgattagc ccgattgttt gcgtcctggg
    15061 ttgatgaatc gcctgactgg gaattgatgg ctcccgttcc cctcggactc gtatgcttcc
    15121 gcgcctcgca aggcgccgcc gatctcgact ctttaaatga atcaatcatg cacggcgtca
    15181 acgcgagcgg ccgcgccctc ctctcgcaca cacgcctcaa cgacaaactc accctccgcc
    15241 tcgccatcgg caacatccgc accaccgaac agcacgtgcg caacgtctgg aagcttttaa
    15301 acgagcaact caaggcgtaa gatatgtgca aaccttgaaa gtagacgttg aacagatcaa
    15361 gacggtgctg ggccggtgtt tgagattacg ttgtcctgcg tgtggtgcgt cccggatagt
    15421 cacccacccg ttccacatcc gccatcactg cccaagctgc atggcgcttt tcaagcgtga
    15481 ggacggtttc tttgtgggcg cgattctcgc aaacgtcgtt accaccgaga tcgtgattct
    15541 ggtcgtctgc ttcttcttcc tgttgattgt cggtgctgac tacgagaacg tgctggtgct
    15601 gttgtttgtg atggcgctga tctttccagt cgccttttac caccacagtt ggagcctctg
    15661 gctcggattc gatttccttg tcgaatcgct gccaaagttt gatcccacaa aaaggcacaa
    15721 aagcacttaa gccacaaaag gcacaaaaag cacaaaaaat aaactcactt gagcgttcat
    15781 tcatttcagc ttttgtgctt tttgtgcctt ttgtggctaa acgtctttct ccttttttgc
    15841 gttttgtgcc tttttgtggc tatactccgc tgagttcgcc cgccagtccg tccagacagg
    15901 ttccgagcac aagtgaactt gctccctgtt aatagacagt gccggcgact acaagtttcg
    15961 tatttgaaag agaattatgc gccactattt caggcgccat cccacaaata cccacgttaa
    16021 tccctgcaca gtgacgcctc gcccatcggc ttgcaggcgt agccctgtgc ctcaactctt
    16081 gaagaaggct ggtaatggca aaacaactaa ttgtgagcgt caacggacgc gagaagaaaa
    16141 ttgcgatcat tgaggatgac aaggtctctg agttctacat agaacgtgga gaacaacacc
    16201 agggcatcgt cggtaacatt tacaaaggac gcgtaatgcg ggttctaccc ggcatgcagt
    16261 ccgctttcgt cgacatcggc ctcgaacgcg acgccttcct ttacgtttct gacttcttcg
    16321 acgaagaaga agagttcgaa cgcatcgtca tcgacaagtc gaagaagggc acgcccgacg
    16381 cggaacacgc agccgccgag gaagtagctc tcaaccgcgt cgaacgcgaa caacacatcg
    16441 atgcaactca ggaacgcgcc gagccgctac tgtccgagat cgaagccccg gttgccgctg
    16501 ccaccgaaga agtctcagaa ccacaggaca aagaccgtgg tcgacgcggc cgccgcggac
    16561 gacgtggacg gcgttgggag aagggcgacg aacccgcacc tgcaactcca ccgccagctc
    16621 cgcgtttcga agttccagac acaccgttcg tcgccggcga tccctcgatc gagcgcatcg
    16681 tggatgagga agagaccgcg ctcaacggcg agatgttcaa agacgctcgt ctccaggaac
    16741 gcctgttcga tcagattcac gccgtcgaat tcaacatgga agacgacttc cgcaccgccg
    16801 aagtcggctc gatactcacg agcggattcg gtagcgagtc gtttgaacgc gttgctgatg
    16861 acgaaggcga tgctgagccg attcgcgagg gaagtagacc accggaagcc cacgtctcga
    16921 cgttcatcga cgaccttggc tcggagacca tgcccagcgt cggatttcaa cgcatcagtg
    16981 acgacgaaga agaagctgta gggcagcaca tcgaagagaa gccgtcgaaa cgaccgcgca
    17041 aagctgctgc gggcggtaaa aagcgtccct cgaaagcggc tgcgactaag acagcgtcga
    17101 agaagaaagc agcgagcaag aagagctcga agaaacgggc cgcaagcacg gagagtgatg
    17161 agagcagcga agccatgact tatggcgact ccgaagcacg cgtcaccgag agccagtcgc
    17221 gtggtgagtt tgccacgcgc cgcggtggtc gcagacgtcg tcgtcccggg aagggcaagc
    17281 cgccatctgc gcccgcggcg gagacggacc atcaggtaaa cgggcaggcg aacggcgaag
    17341 acgatgagat cgaggcgata cagtttacag agccggaaga gatcgtagct gctcctccgc
    17401 cagcgccagc ggcggcgccg gagccgagtc cgcgccaacc acgcgggcgt gaccagcgtg
    17461 aaaaacgcga tcagcgcggt tatcgtggta accaatcacg acgcgagcga caccaaccgg
    17521 cgatcacgga tttgctcagc gaaggtcagg agatactcgt ccagatcgcg aaagagccga
    17581 ttgctaagaa gggcgcgcgc atcacgtcac acatcgcttt gccgggacgt ttcctcgttt
    17641 acatgccgac tgtcgagcac gtcggtgtct cgcgcaagat cgagtcagac aacgagcgtc
    17701 atcgtttgcg taaattaatt cagacgcttc gtgctgaaga agatattcct tcgggcggat
    17761 tcatcgtgcg cactgcgggc gttggcatca gcgagaaaga gctgcgcgaa gacgttcgtt
    17821 acctcgtacg gacgtggctc gatattcgtg ctgctgccga gaagtcgaaa gcgccgtccc
    17881 tggtccatcg cgatttggac ctggtccaga ggctgttgcg cgatcagttg tcagacgact
    17941 tcgaatcgat tcgcgttgat tcggaggagg agtaccaggc gatcgtcgag tttatcaatc
    18001 gcattcagcc gcgtttggtg aagcgcgtca agctctacac gaaggaccag ccgatactcg
    18061 aagcttatgg agtgcaggtt gagatcgaca aagcgatcaa gccgcgtgta tggctgaagt
    18121 ccggcggtta tctcgtgatc aatcaaaccg aggctttggt cgcgatcgat gtgaacacgg
    18181 gcaagtttgt tggtcggggc agtggtcgac tcgaagacac gatcacgcgt acgaacctcg
    18241 aagctgttga cgagatcgtg cggcagattc gtttgcgcga tctcggcggc atcatcgtgc
    18301 tcgacttgat cgacatggaa gagcgtcgca atcgtcagaa agtgatgcag gcgctgcagg
    18361 aagctttgcg taatgataag tcgccgacga aggtgctttc attcaacgac ttcggactcg
    18421 tgatcatgac gcgcaagcgt gtgaagcaat cgctcgagcg gacgctgtgt tcgccgtgtc
    18481 cgaactgcca gggtgccgga ctcatcaagt cgccgcagac gatctgttac gagctgctgg
    18541 aggaagcgcg gcggttggcg aagggaatca acggcgacaa ggacaacaag caggtcacgc
    18601 tgcgaatcaa tcctgatgtg gcgaaggcgc tacgcaccag cgagcgcgac gtgatgatcg
    18661 agatcgagga ctatcttggc ccaatcgaca tcacgagcga cgagacgata caccaggagc
    18721 agtacgactt cgctgtaact tagccacgga ttagacggat catttgccac ggatctacac
    18781 ggataaagct aaatccgtct tttccgtgta atccgtgtta cggtttacgt gtttgtaaag
    18841 tatgtccctt tgttggggaa tgaagccggc ctcacgaatc aaaaactgca gcatgttctc
    18901 atcagcgcag ttgtgagcgc cggcggcgga gacgacgttc tcctcgatca tgatcgagcc
    18961 catgtcgttc gccccgaaac gaagcgcgac ctgcccgagt cgtaaaccct gcgtcaacca
    19021 cgacgactga aagttctcga tgttgtcgaa gaacagccgc gataccgcca gcatcttcag
    19081 ataatccacg ccagtcggtt cgtccttaat tctgcgacca agcgctgtgt tctcacgttg
    19141 aaacgtccac ggaatgaacg ccgtgaagcc gcccgtttcg tcctgaacat cacggatgcg
    19201 ctgcaagtgt ctgacgcggt gttcgatctt atctccgatc ccaaacatca tcgtcgccgt
    19261 cgatctcagc ccgacccgat ggaccgcgcg catcacgtcg agccattcgt cagtcgtaca
    19321 cttcgtcgag actcgcttgc gaacttcatc gtcgaggatc tcagcgccgc cgccgggcag
    19381 actgtagagt ccggcgtctt tcaagcgcgc catgatcgtc tcgtaatcga gtttcgagat
    19441 taggtggatg ttgtgaatct ccggcggtga gaaacagtgc aactgaaatc ccggatactt
    19501 tgcgcgcagc gagcgcaaca gatcttcgta ccattcgatg ccgaagtcag gatgcagacc
    19561 accctgcatc aacacgcccg taccgccgag cgcgatcgtc tcgtcaatct tggcgcagat
    19621 ctcgtcgaac gtgcggacgt aagtgtcggg cgcgcccggt cgacgataga acgcgcagaa
    19681 cgtgcacacg acgttgcaga cgttcgtgta attgatattg cgatcgatga tgtaggtgac
    19741 gatgttatcc ggatggcggc gcgcgcgaat ctcgtcagca gcgacgccca tccgcgcgat
    19801 gtcgtgcgat tcgaggaggc gggtgcactc atccgtggtc atccgctctc cggcgagcac
    19861 ccggtctaag atcgattgaa tgttcgaaga catatccgtg ttatccgtgg ctaaataagt
    19921 ctcaatggct ttacgcgttc gatgagtcca tgcttgaaag ccagttcaaa atagagccgc
    19981 agccctcttt ccatcgactc atctactctg aaaacgatgt tctcagtcaa gtagtttcgc
    20041 agctcctcga ctcgcatcgg gattttatcc tgatacgact gcacgatctc ctcaatatga
    20101 gcgacgccct cgtcgcgtgc gcccgcgaag tcgatttcac gtgcacgcga gacagcctcg
    20161 tcacgcacca tccacatcgc aaaaacaaac ccgagtccgg tgtgttcttt ccagagattc
    20221 gccatatccc aaacgttcag tccctgccgc ggaaacgcca tacccggatc gccgatgatc
    20281 aacgccgcgt cgttcttctc cagcatctct tcaaggttcg gtgcgcgtgt ggtccactca
    20341 ggctcgcgct caagaaactc tctgaagatc actttcacga gcgtcgccga agtacgcgac
    20401 gactcatcga gcgcgacgct ttgaatgttc tcaagtcgtt tattcttcga cacgagtacg
    20461 acgctcaaca cttctttctg cgagccgaca cacacgttcg ggacgagcga gccaccatca
    20521 atgcgttgat actcaatcac cggcacgagc gcgccgtcga cctcacttct cgacagcagt
    20581 tgcgcgcagc gcgccggcac agcttcgaca aactcgactg tgtcttcgtg tggtccgtgt
    20641 aaaaagctcc agattaaagg cgctgaattt aagtagcttg aagcggcgag tcgaaagggc
    20701 gcgttcatgt agtcgcaagt atattcgatt gaactgccgg atcaaattga catactatgc
    20761 gccggtgccc atttacgaca tcagcgttcc catcagcaac gaattgccaa cctggcccgg
    20821 tgatccaagc gtgcagctta ccgtctggcg cagcctctcg tcgggcgacg aggccaacgt
    20881 ttccatgctc aacttcggcg cgcacaccgg cacgcacgtc gacgcgcccg cgcatttcat
    20941 cgaaggcgct gcgaagatcg aatcgttgcc gctcgaagtg ttgattggcg cagtgcaagt
    21001 gatcgaggtt ccggacgatt tccacgccat caatgaagag tttgttctca ctaactgcgc
    21061 tccggagaca acgcgcgtac tcttcaaaac tcgcaactcc gcattctgga gcgagagcga
    21121 gcatgtattt cataccgact tc
//
LOCUS       DASSFQ010000172         4184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_77479, whole genome shotgun sequence.
ACCESSION   DASSFQ010000172 DASSFQ010000000
VERSION     DASSFQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77479"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFH96_06015"
     CDS             complement(<1..272)
                     /locus_tag="VFH96_06015"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HET6973561.1"
                     /translation="MASKRLIRVGVDTGGTFTDFVFEQDGRLQLFKLPSTPSDPSLAI
                     QDGLARICAETGSQLDKIEVVHGTTVGTNALLQRRGARTALITTKG"
     gene            complement(262..1356)
                     /locus_tag="VFH96_06020"
     CDS             complement(262..1356)
                     /locus_tag="VFH96_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6973562.1"
                     /translation="MKKSPVGIGIIGTGFARTTQIPGFRDCMGAKVVAIASRNRDRAE
                     AIAKEFGIEHVATDWHELVAHRDVDLVSVVTPPSTHMEIALAALDQRKAVLCEKPMAL
                     NAGEAARMVEKANAAGVLALIDHELRFLNSRRVMRGMLESGVIGTVRHCNYVFRSDYR
                     GIADRAWDWWSDETMGGGALGAIGSHVVDSFRWMLGAEVSQAFGMLTTHIKQRRDKDN
                     GGLLPVTTDDEAKLLFKFNDGPHTENATGAASISVLESGKYENRLEIYGTKGALMVEE
                     TGELWRSPTGSGTWRPVQVDQDHMAQGMREGSWSRGFTGFSIAICEAMRAGRTTVKDG
                     ATFADGYRVQLVLDALRASNESGCWVTVGQ"
     gene            complement(1404..3392)
                     /locus_tag="VFH96_06025"
     CDS             complement(1404..3392)
                     /locus_tag="VFH96_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973563.1"
                     /translation="MSATQVNVDRPQFGQVYMWSLVIAGAAIALGSLLVLPFHKLDSG
                     FFVLCLMVIASSLIAIRIPRVSGRITVADTFVFLTMLLYGGAAAILVSALEGVAATLI
                     ISKRPRTILFNSAILATSTFFTSTVLNLVVGPPAAIAEAGRFSGPFFRAICLMALVQY
                     VANTTLIAIEKASKIKESTWNTWRTYYLWTSVTYFAGASAAGIIAILIKTYSFYAVIA
                     TVPIILIICFTYQTYLKNLEASLEQTEAARLHVQELSKYVTELQRSEEAREQLLVRAE
                     RARSEAEAANRIKDEFLATLSHELRTPLTSLLGWSSVLREAKRDEKVLHQGLEAIDRN
                     AKVQAQLIDDLLDVSRIVSGKLNLDVRPLDLSSVARAAINVVRPAADAKGITLDYSDE
                     PGLGAISADSARLHQIIWNLLSNAVKFTPHGGKIFVRVERTGSDARITVSDTGQGIEP
                     EFLPRVFDRFRQADSSTTRSFGGLGLGLAIVRHLVELHGGTVSAQSDGLKKGATFTAT
                     FPLLADRTEPVAVAHCEQPLAEMYSLDGLRVLLVDDEAEARQIISTVIMRTGAEVKAC
                     TSAQEAFAKLLEWKPDVILSDIAMPDEDGYSFIGKVRSLPRDKGGETPAAALTAYARD
                     VDRRQALAAGYQMHIAKPIGASQLVTMIARLAGRDTDS"
     gene            complement(3427..>4184)
                     /locus_tag="VFH96_06030"
     CDS             complement(3427..>4184)
                     /locus_tag="VFH96_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6973564.1"
                     /translation="GVSGAANRRMMYLSGTSMATPVAAGTAALMLQANPSLTPNMVKA
                     LLMYTAQPLANYNMLEQGAGQLNTAGAVQLAQLVRTDLSVSTTVGAPLLTADPPAPET
                     TIANQTFTWSRGIILNYTYATGIDLITKYQQVYALGNLLNSGVITGDGVITGDSSYLS
                     DGVITGDQILTSSGVITGDGSAFLAVSLLFQDLLPEGSLLSDGVITGDGVITGDGVIT
                     GDLVASAQSAMLNGEGSSTMAAPSDDGNDCLDY"
BASE COUNT          902 a         1230 c         1147 g          905 t
ORIGIN      
        1 aaacccttgg tcgtgatcaa cgccgtgcgt gcgccgcggc gttggagcag cgcgttggtc
       61 ccaacagttg tgccgtgtac gacttcgatt ttatcgagct gtgagccggt ctcagcgcag
      121 atgcgcgcga gtccatcttg aatcgcgagc gatggatctg aaggtgtcga gggaagtttg
      181 aagagttgaa gacggccgtc ttgttcgaag acgaaatctg taaacgtgcc gccggtatcg
      241 acgccgacgc ggatgagtcg tttactggcc aaccgttacc cagcacccac tttcattcga
      301 agcgcgcaac gcgtcaagca caagctgcac tcgataaccg tctgcaaaag tcgccccgtc
      361 tttcacggtc gttcgtccgg cgcgcatcgc ttcgcagatg gcgatcgaga atccagtaaa
      421 gcctcgcgac cagcttcctt cgcgcattcc ctgggccatg tgatcctggt ccacctgcac
      481 cggacgccac gttcccgagc ctgtcggcga gcgccacagt tcgccggttt cttcaaccat
      541 cagcgcgcct ttcgtgccgt agatctcgag ccggttttcg tatttgccgg attcaagcac
      601 ggagatcgat gccgcgccgg tggcattctc cgtgtgtggg ccatcgttga atttgaacaa
      661 tagctttgct tcatcgtccg tggtgacggg aagcaagcca ccattgtctt tgtcacgacg
      721 ctgtttgatg tgtgtcgtga gcatgccgaa cgcttgcgac acttcagccc ccaacatcca
      781 ccggaatgaa tcgaccacgt gcgatccaat cgctccgagc gcgccgccgc ccatcgtctc
      841 gtccgaccac caatcccacg cccgatccgc aataccacga taatcggatc gaaacacgta
      901 attgcagtga cgcaccgtgc caatcacgcc cgattccaac attccgcgca tgacgcgccg
      961 gctgttcaaa aaccgtaact cgtgatcaat cagcgccagc acgccggcgg cgttggcttt
     1021 ttcgaccatg cgcgcggctt cgccggcatt cagagccatc ggtttttcgc aaagcacggc
     1081 tttacgttga tccagcgcgg cgagcgcgat ctccatgtgc gtcgacggcg gcgtgacgac
     1141 gctcacgaga tcgacatcgc gatgagcgac gagttcatgc cagtcggtgg cgacatgttc
     1201 gatgccaaac tctttggcga ttgcttcggc gcggtcgcga ttgcggctgg caatcgccac
     1261 gaccttcgct cccatgcaat ctcgaaaccc gggaatctgc gtcgtgcgag caaaccccgt
     1321 cccaataatt ccgataccta cgggcgattt cttcatggca gtgttattta gccacagatt
     1381 acacagattg caagaccaaa tatctaagaa tctgtgtcgc ggccagcgag gcgggcgatc
     1441 atggtgacga gctgactggc gccaatcggt tttgcgatgt gcatctggta gccggcggcg
     1501 agtgcctgac gccgatcgac gtcgcgggca taagcagtga gtgcggcggc gggcgtttcg
     1561 ccacctttgt cgcgcggcaa cgaacgcact ttaccgataa aactgtagcc gtcttcgtcg
     1621 ggcatggcga tatcgctcag aatcacatcc ggcttccact ccagcaactt cgcgaacgcc
     1681 tcttgagctg acgtgcaagc tttcacttct gctccggtgc gcataatcac tgtgctgatg
     1741 atttgtcgcg cttccgcttc gtcgtcaacc agcaataccc gcagaccgtc gagtgaatac
     1801 atctccgcga gcggctgctc acaatgagcg acggcaaccg gctcagttcg atcagccagc
     1861 agtggaaatg tcgcggtgaa ggttgcgcct ttcttcaggc catcactctg cgcagaaacg
     1921 gtgccgccat gcagttccac caaatgccga acaatcgcca gccccaatcc taagccgcca
     1981 aaacttcgcg tcgtcgaact gtccgcctga cgaaatcgat cgaatactcg cggcaaaaac
     2041 tccggctcga ttccctgccc tgtatcgcta acggttatgc gcgcgtctga tccagtgcgt
     2101 tcgacacgca cgaagatctt gccgccgtgt ggagtgaact tcacggcgtt ggataacagg
     2161 ttccaaatga tctggtgcag tcgggcggaa tcagctgaga tggctccgag accgggttcg
     2221 tcggaataat cgagcgtaat gcctttcgca tccgcggctg gtcgcacgac gttgatcgcg
     2281 gccctggcga ctgaagacag gtccagtggc ctgacatcga gattcaactt gcctgaaacg
     2341 atgcgcgata cgtcgagcag atcgtcgatt agttgggcct gaactttcgc gttccgatcg
     2401 atggcttcga gtccctggtg caacactttc tcgtcgcgtt tcgcttcacg caacacgctg
     2461 gaccagccaa gcaatgaagt cagcggcgtt ctcaattcgt gcgagagagt cgcgagaaat
     2521 tcatccttga tccggttcgc cgcttcggct tccgagcgcg ctcgctcggc acgcaccaac
     2581 aactgctcgc gtgcttcttc ggagcgttgc aactcggtga cgtatttcga tagttcctga
     2641 acgtgcagtc gtgctgcttc ggtttgttcg agcgaggcct cgagattttt gaggtacgtc
     2701 tggtacgtga aacaaatgat gaggatgatc ggaactgttg caattaccgc atagaagcta
     2761 taggtcttga tcagaatcgc aatgatgccc gccgccgacg ctccggcgaa ataagtcacc
     2821 gaggtccaga gataatacgt tcgccacgta ttccacgtag attctttaat tttgctggct
     2881 ttttcgatcg caatcagcgt ggtattagca acgtattgca ccagcgccat caagcaaatc
     2941 gcgcgaaaga acgggcctga aaacctgccc gcctccgcaa ttgccgcggg tggaccaaca
     3001 accagattca aaactgtcga agtaaaaaag gtcgacgttg cgaggatcgc tgaattgaaa
     3061 aggatcgtgc gtgggcgctt gctgatgatc agtgttgctg caacaccttc gagcgccgac
     3121 acgaggattg ccgccgcgcc cccgtagagg agcatcgtta ggaaaacaaa agtatcggcg
     3181 acagtaatgc ggccactaac gcggggaatg cggatagcaa tcagcgaact cgctatcacc
     3241 atcaggcaca gaacgaagaa gcctgagtcc agcttgtgaa aaggcaatac cagcagtgag
     3301 cctaacgcga tcgctgctcc ggcaattacc agcgaccaca tgtagacctg gccgaactga
     3361 ggccgatcga cgttcacttg cgtcgcgctc attgttcttc ctctgtgtgt aacgtgttgc
     3421 tgtggactaa taatccaggc agtcgttgcc atcgtctgat ggtgccgcca tcgtcgaact
     3481 gccttcacca ttgagcattg ccgactgggc gctggccacg aggtcgcctg tgattacgcc
     3541 gtcgccggta atgacaccat cccccgtgat caccccatcg ctcaacaaac taccttcggg
     3601 caagagatcc tgaaacagca ggctgacggc cagaaaggcc gagccgtctc cagtgataac
     3661 gccagaactc gtaaggattt gatcgcccgt gataacgccg tcagacaagt atgaagaatc
     3721 gccagtgatc acgccgtcgc ctgtgattac gccgctgttg agcagatttc cgagagcata
     3781 gacctgttga tacttcgtga tcagatcgat accggtggcg tacgtgtaat taagaatgat
     3841 gccgcgcgac cacgtgaacg tttgattcgc aatcgtggtt tccggagccg gcgggtcggc
     3901 agtaagcaac ggcgcgccga cagtggtcga aacactcaaa tcagttctca cgagttgagc
     3961 caactgcaca gcgcccgcgg tgttcaactg tccggcgcct tgctcgagca tgttgtagtt
     4021 cgccagcggt tgggccgtgt acatcaacag cgccttaacc atgttcggcg tgagactcgg
     4081 attggcctgc agcatcaacg cggctgttcc cgccgcgact ggcgtggcca tagaagtgcc
     4141 actcaaatac atcatcctgc gatttgcagc gccactgact ccgg
//
LOCUS       DASSFQ010000173         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1110396, whole genome shotgun sequence.
ACCESSION   DASSFQ010000173 DASSFQ010000000
VERSION     DASSFQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110396"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..815
                     /locus_tag="VFH96_06035"
     CDS             27..815
                     /locus_tag="VFH96_06035"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6973565.1"
                     /translation="MKCLHLLVILLVLVIPAVAQKSGAFPSGRIVDLTYAFDANSVYW
                     PTAEQFKLETDFEGMTDKGYFYSAYRYSAAEHGGTHLDSPVHFAKGRYTVDQLPLEQL
                     MGPAIVIDVTAKCATNPDYLVSVADFENWEKRNGKIPAGTIVLLRTGFGKFYPDRKKY
                     LGTEERGAEAVAKLHFPGLDPAAARWVTTNRSIKAIGLDTASIDYGQSTLFESHRALF
                     EKNVPAFENVANLDQLPAKGFSVIALPMKIKGGSGGPLRIIAVL"
     gene            821..1504
                     /gene="pgl"
                     /locus_tag="VFH96_06040"
     CDS             821..1504
                     /gene="pgl"
                     /locus_tag="VFH96_06040"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET6973566.1"
                     /translation="MIEIYSNAEELARAAAEYFVAQCPETVALSGGSTPKLMFQVLAD
                     QFRDEVAWSNIQFFWSDERHVPPDNAESNYRMANEALLSHVPVSANNVHRIRSENPDA
                     AAVASEYEQTIIDVTRQTLPRLDLIFLGLGTDGHTASIFPGSEVLHETKRLVAAPYVE
                     KFKSYRITMTLPLLNNGASIVFLVSGAEKAEIVKQVIQGEHKYPAQAVKPTQGELIWM
                     LDKDAASKL"
     gene            complement(1569..3290)
                     /locus_tag="VFH96_06045"
     CDS             complement(1569..3290)
                     /locus_tag="VFH96_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET6973567.1"
                     /translation="METKVDAALRRLVATNLVAILFTDLAGNILNADDSFLNLLGYTS
                     TNLPSSIHDLTPPEHHRLDEEAFEKLMAFGACAPFEKDLLTHDGTPLPVLFGAVMHDE
                     EIACFVVDLSQNKQTQEKLNHLAYHDALTDLPNQVLFKDRLRQAIALSRRNDHMQAVL
                     LLNLDRFKTINDSLGYTAGDRLLQSVAQRLTSCVRESDTVARFGGDEFAVLLTNIPRA
                     QDAANVARAIKQALDQAFIFEDQEICVASSIGISMYPHDGRDTAGLLKTAGVALDRAK
                     VQGGNNFHFYTAGGTTRALKQLVLESNIRPGIERSEFFVQYQPQVDIRGFQVVGMEAL
                     VRWQHPGLGLLYPSEFVTIAEETGLIIALGDFVMRTACAQNKNWQDKGLQQMRISVNF
                     SARQFQQPTFIADVSHILKETNLDPRWLELELTESSIMKDPDEAIEKLHELKLMGINV
                     AIDDFGTGYSSLNYLKRFPIDTLKIDKTFVSDVCKDPHDTAIVRAIINLGHALDLTVI
                     AEGVETKEQLQYLSALECDVVQGFLFSKALPAKAFEELLMEQRRVTSGNVSPTVPLSI
                     DSLVPAS"
     gene            complement(3424..>4067)
                     /locus_tag="VFH96_06050"
     CDS             complement(3424..>4067)
                     /locus_tag="VFH96_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6973568.1"
                     /translation="LWHQMLAQQGYIVMSVDNRGTPAPRGRAWRKIIYRKMGIVNSGD
                     QAAAARAVAKWPFVDPARIGIWGWSGGGSSTLNAMFRYPDVYATGMAVAPVPDLHYYD
                     TIYQERYGGLPKDHPEEWKQSSPITFAGQLKGNLLVVHGTGDDNVHYQGTQALINALV
                     AANKQFSMFAYPNRSHSISEGPGTSRHLMGLLTRYLNDKMPPGPATSATSSVR"
BASE COUNT          925 a         1111 c         1130 g          901 t
ORIGIN      
        1 aatcctaacg cgaaggagca attcagatga agtgtttgca cctgcttgtc attttactgg
       61 ttctcgtaat tcctgcggtg gcgcagaaat ccggcgcgtt tccctcggga cgtatcgtcg
      121 atctcaccta tgcgttcgac gcgaacagcg tctactggcc cacggccgag cagttcaagc
      181 tcgaaacaga tttcgaaggg atgactgaca agggttactt ctactcggcg tatcgttact
      241 cggcggcgga gcacggcggc acgcatctcg attcgcccgt gcacttcgcc aaagggcgct
      301 acacggtcga tcaattaccg ctggaacaat tgatgggtcc cgcgatcgtg atcgatgtga
      361 cggcgaagtg tgcgacgaat cctgactatc tcgtgagcgt ggctgatttt gaaaactggg
      421 agaaacgcaa cgggaagatt ccggcgggca cgatcgtctt gctgcgcaca ggctttggaa
      481 agttttatcc ggatcgaaag aagtatctcg ggactgaaga gcgtggcgcg gaagcagttg
      541 cgaagttaca ctttccggga ctggatcccg cggccgcacg ctgggtcaca acgaatagat
      601 cgatcaaggc gatcgggtta gacacggcga gcatcgatta cgggcagtcg acgttgtttg
      661 agagtcatcg ggccttgttt gagaagaatg ttccggcgtt cgagaatgtg gcgaacctgg
      721 accagttgcc ggcgaagggc ttctcggtga tcgcgttgcc gatgaagatc aaaggtggaa
      781 gtggtggacc gttgcgaata attgctgtgt tataggtctt atgatcgaaa tctactcaaa
      841 tgctgaggaa ctcgcgcggg cggcggcgga gtattttgtt gcacagtgtc cggagactgt
      901 ggcgctgtcg ggcgggtcga cgccgaagtt gatgtttcag gttttggccg atcagtttag
      961 agatgaggtg gcgtggtcca atattcaatt cttctggtct gacgagcgac acgttcctcc
     1021 tgataacgct gagagcaact atcggatggc taacgaggcg ctgctgtcgc acgttccggt
     1081 ttcagcaaac aacgtacatc gcattcgcag cgaaaacccg gatgccgcgg cagtagcgag
     1141 cgagtacgaa caaaccatca tcgatgtaac aagacaaacc ttgccacgtc tcgatctgat
     1201 cttcctgggt ctcggaacgg acggccacac cgcttcgatc tttccaggtt ctgaagtgct
     1261 gcacgaaaca aagcgcctcg tcgctgcgcc atatgtcgag aagttcaaat cctatcgcat
     1321 cacgatgaca ttgccactgc tgaacaacgg cgcatctatt gtctttctgg tgagcggtgc
     1381 ggagaaggca gagatcgtaa aacaagtaat tcaaggcgag cacaaatatc cggcgcaggc
     1441 cgtgaagcca acgcaaggtg agctgatctg gatgctcgat aaagacgccg cgagtaaatt
     1501 gtagacaaga tttacaagat ttttcaggat agaacatgta aatcctgaaa catcttgtta
     1561 atcctgtctc aactcgcagg taccagcgaa tcgatcgata agggcaccgt cggcgaaacg
     1621 tttcctgacg tcacccgccg ctgctccatc aacaactctt caaacgcttt ggccggtaac
     1681 gccttgctaa acaaaaaacc ctgcacgaca tcacactcga gcgcactcag atactgaagc
     1741 tgttcctttg tctcaacacc ttctgcgatc accgtcagat ccagcgcgtg tccgaggttg
     1801 atgatcgcgc gcacgatcgc cgtgtcgtgt ggatctttgc aaacatcgga aacgaatgtc
     1861 ttgtcgatct tcaacgtgtc gatcggaaaa cgcttgaggt aattgagcga cgaatagccg
     1921 gtgccgaagt cgtcaatcgc gacgttgatg cccatcaact tcaactcgtg cagcttctca
     1981 atcgcctcgt ccggatcctt catgatcgag ctctctgtca gctcgagctc gagccagcgc
     2041 ggatcgaggt ttgtctcttt cagtatgtgc gacacatcgg cgatgaacgt cggctgctga
     2101 aactgccggg ccgagaagtt caccgagatg cgcatctgct gcagtccttt gtcctgccag
     2161 tttttgtttt gtgcacacgc cgtgcgcatc acgaagtcgc ccaacgcgat gatcaagccg
     2221 gtctcttcgg cgatcgtaac gaactcgctc ggatacaaca gacccaaacc cggatgctgc
     2281 cagcgcacga gcgcttccat cccgacgact tgaaagccac gaatatcaac ctgcggctga
     2341 tactgcacga aaaattcaga acgctcgatc cccgggcgga tattactctc gagcacgagc
     2401 tgtttgagcg cgcgcgtcgt gccgcccgcc gtgtagaaat gaaaattgtt gccgccctgc
     2461 accttcgcac gatcgagtgc gacgccggct gtcttcaaga gtcccgcggt gtcgcgaccg
     2521 tcgtgcggat acatgctgat gccgatactg ctcgctacgc agatctcctg atcttcaaag
     2581 atgaatgcct gatcgagtgc ttgcttgatc gcgcgcgcga cgttcgctgc gtcctgcgcg
     2641 cggggaatgt ttgtaagcag cacggcgaat tcatcaccac cgaaacgcgc gaccgtatcg
     2701 ctttcgcgaa cacagctcgt caatcgctgg gccaccgatt gcaacaaacg atcgcctgcc
     2761 gtgtagccga gcgaatcgtt gatggtcttg aagcgatcga gattgagtaa caacaccgcc
     2821 tgcatgtggt cgttgcgacg agaaagcgcg atagcctgcc gcaatcgatc tttgaacagc
     2881 acctggttcg gcagatccgt cagcgcatcg tgatacgcga ggtggttgag tttttcctga
     2941 gtctgcttgt tctgagagag atcgacgacg aaacacgcga tctcttcgtc gtgcatcact
     3001 gcgccaaaca aaaccggcaa cggtgtgccg tcgtgtgtca gaagatcttt ttcgaaaggc
     3061 gcacacgcgc caaacgccat cagcttctcg aacgcttctt catcgaggcg atgatgttcg
     3121 ggcggcgtta gatcgtgaat gctggaaggc aggtttgtgg aagtgtagcc gagcagattc
     3181 aggaaactgt cgtcggcgtt gagaatgttt ccggcaagat cggtaaataa aatggcgaca
     3241 agattggtgg cgacgagacg ccgcagggct gcatcgactt tggtttccat accatgaatg
     3301 agaattcgct gaatttagga aaggtgagta ccgggcaagc gggttggccg tcaacccgaa
     3361 gttaggctac cacagattac gcagatgaca cagaaaaatc tgcgtaatct gtggatgtgt
     3421 cggttaccga acagaactcg tcgcgcttgt tgctggtccc ggcggcattt tgtcgttgag
     3481 atagcgcgtc agtagtccca tcaagtgtct cgacgtcccc ggtccctccg agatcgagtg
     3541 tgatcgattt ggatacgcaa acatcgaaaa ctgcttattc gccgcgacca gagcgttgat
     3601 caacgcctgc gtgccctggt aatggacgtt gtcatcgccc gtcccatgca cgaccagcag
     3661 attgcccttc aactggcccg cgaacgtgat cggcgagctc tgcttccact cctccggatg
     3721 gtctttcgga agtccgccgt atcgctcctg gtaaatcgtg tcgtagtaat gaaggtcagg
     3781 caccggcgct accgccattc ccgtcgcata aacatccgga tagcggaaca tcgcattgag
     3841 cgtcgacgag ccgccgccgc tccaacccca gattccgatg cgcgccggat cgacaaacgg
     3901 ccacttcgcg accgcacgcg ctgccgccgc ctgatcgccc gaattcacaa tacccatctt
     3961 gcgatagatg atcttgcgcc acgcacggcc gcgcggcgca ggcgtgccac gattatcgac
     4021 actcatcacg atataaccct gctgcgccag catctggtgc cagaggc
//
LOCUS       DASSFQ010000174        20045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1705840, whole genome shotgun sequence.
ACCESSION   DASSFQ010000174 DASSFQ010000000
VERSION     DASSFQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20045
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705840"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFH96_06055"
     CDS             complement(<1..612)
                     /locus_tag="VFH96_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973569.1"
                     /translation="MSLARAIHDAGGRALLVGGCVRDALMGVQPKDWDLEIYNLDAAR
                     LREVLDQFGPVNVVGEAFTVYKLGNHLDVSIPRRERKSGRGHKGFVIEGDPSMSVAEA
                     AHRRDFTINAILEDPLTGELIDPFEGRRDIEQRVLRAVSKDTFAEDSLRVLRAAQFAA
                     RFEFDIEPETIELCRTIDLSDLPAERIWGEIEKLLLRAIHPSIG"
     gene            complement(648..1013)
                     /locus_tag="VFH96_06060"
     CDS             complement(648..1013)
                     /locus_tag="VFH96_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973570.1"
                     /translation="MPVVKKRNQQTETRLLLESEDPVELPPAEIDDNAVLVQFEKFKP
                     YIPELLETLRAGTVAEPVPVMIDGSTFAIHGPTRTFWVKLWHAAGSDMTKIDRAQVIS
                     CLPSTRGVQILVESPEEDP"
     gene            complement(1020..2408)
                     /locus_tag="VFH96_06065"
     CDS             complement(1020..2408)
                     /locus_tag="VFH96_06065"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET6973571.1"
                     /translation="MPAPNIDTIVSLAKRRGFVFPSSEVYGGLGSAWDYGPLGVELCN
                     NVKQAWWRWLVYERDDIEGIDSSIILNRLVWKYSGHEATFNDWLIDCRKCKGRFRADK
                     IVDNKCPSCGASDWTEARPFNTMLQTKLGAVADDDPTALAYLRPETAQGIFINFLNVQ
                     TTMRRKLPFGIAQVGKSFRNEVTPGNFILRTREFEQMEMEYFVSPTDEHFKLVDYWVE
                     QYLEWFATLGVNRENLELYEVPNEELAHYSKRTVDIMYRYFPEREKPWEELMGIAWRT
                     DFDLGIHSKNKLDAEGKRLNPDSTEDLSYFDEAAKQRFYPHVIEPAAGVNRTVLAVLM
                     DAYSEKTNDKGETRVILKLSPQLAPIKAAILPLAKNKPEIVGMARAIKRSLQPVLRAV
                     YDDTGGIGKLYARQDEIGTPFCITVDHQSLEDEAVTVRDRDTWEQERIKVADLVEHLQ
                     KRLKPYPVADSI"
     gene            complement(2540..2704)
                     /locus_tag="VFH96_06070"
     CDS             complement(2540..2704)
                     /locus_tag="VFH96_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973572.1"
                     /translation="MAEEGGGGAGSVGVVAIVVIFVLIVLVGVFMFRNRIFGGGGSQK
                     IDVNIQTPSK"
     gene            complement(2852..3847)
                     /locus_tag="VFH96_06075"
     CDS             complement(2852..3847)
                     /locus_tag="VFH96_06075"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6973573.1"
                     /translation="MSQRSPILRNLKFAFIALFALVFFAVPAPTASAKPKKAKYATIK
                     IQTNPGGLLLMIDGKPHGETTADFRAFDLDAGLHTVQVRLPNGQFWTREVELPAGRVK
                     CVVVNYRPLPPLPKSPCPFPVSISAPNQVTEGEIITYTADVAYSGNAALKYNWVVTPS
                     SAHVISGLGTSTLNIESTGLGGQRIIATLTADDGSSDPACAQSAQAVSTIVPIKKVAI
                     VAREFDECNNCTFDDQKARLDNLAVELQNDPTTRAYIIAYGGRMSPVGQVEKLMNRAR
                     EYMITQRGIDASRLSVVNGGYREEDSVELWIVPSGAAAPQATPTVQAGEIKRRKR"
     gene            complement(3847..4419)
                     /locus_tag="VFH96_06080"
     CDS             complement(3847..4419)
                     /locus_tag="VFH96_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET6973574.1"
                     /translation="MLVVLLTAVPALAEPVKFNQVVQTLTSTQGVIDLRLNTLTQDPK
                     KEGESVAKAKDPKQTDGKTESVISSVVVTGAGKTIGVEEIEDGEVEGTICDCGEIFIA
                     GASFPKWPFLFLAAVPLVFIDRDSDEQPTPTPPPGNSPTPTPTPTPTPNSTPTPTPEP
                     ASLLLFGTGLLAAGAGLRKRYAKSKIKEDQ"
     gene            complement(4852..6996)
                     /gene="pnp"
                     /locus_tag="VFH96_06085"
     CDS             complement(4852..6996)
                     /gene="pnp"
                     /locus_tag="VFH96_06085"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET6973575.1"
                     /translation="MSQRKYLKESIKLGDKELTVESGRVAKQADGSVVIRYGDTMLLV
                     AAVGALTPREGVDFFPLTVEYRESNYAAGRIPGNYFRREGRPNEKEVLTSRLIDRPCR
                     PLFAEGYRNETQVIASVISADPDNNPDVIAITGASCALYLSDIPFMNPIAGVRIGLIE
                     GRYIVNPTYDEVRESRLNLIVAGTEEAIVMVEAGASEVSEEIMVEALMLAHKEINRLC
                     RWQRELYKALEIQKREVTPLELDPEMTREITERYAENLRTALDTSAQEKRASYAAVDA
                     LKKEVVESYPEDQPEKRSMAKLIFDQLKEMVFRDDILNKRRRPDGRRFSEIRPITSEV
                     GWLPRVHGSALFTRGETQALVSTTLGTKEDEQFIDDLEKGEVKRRFLLHYNFPQYSVG
                     EVGRYGSSSRREIGHGALARRALETVLPEDSDFPYTIRIVSDITESNGSSSMATVCGG
                     TLSLMDAGVPLKAPVAGVAMGLVMEGNKYAILTDIAGAEDHYGDMDFKVAGTRNGITA
                     LQMDIKIAGINAQIMAEALEQAKKGRLYILGAMEKTLAEPREEISAHAPRIIQIKINP
                     DKIRDVIGPGGKVIRSLVEETGAKIDVSDDGTISIATASGDAADAAVNRIRGLTAEAE
                     VGQTYLGTVNRIVDFGAFVEIFPGTDGLLHISEIADRRVKDVRDELKEGQQIMVKCIG
                     REGNKIKLSRKAVLREENRKAEAASPSDGGGE"
     gene            complement(7321..7590)
                     /gene="rpsO"
                     /locus_tag="VFH96_06090"
     CDS             complement(7321..7590)
                     /gene="rpsO"
                     /locus_tag="VFH96_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET6973576.1"
                     /translation="MALAKQTKEQIVTDFRTHESDTGSPQVQVALLSKRINDLTEHFK
                     THKKDNHSRRGLLKMVSQRRSLLDYLKRTDIERYHEVVNRLGLRR"
     gene            7835..9067
                     /locus_tag="VFH96_06095"
     CDS             7835..9067
                     /locus_tag="VFH96_06095"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6973577.1"
                     /translation="MAKAKIVILGGGFGGIFTALELAGAADVTLVSDADHFLFTPMLY
                     EYLSGEVEAWHIAPRYDELLEENVHSVKSAATGIDLKSQSVSLANQQTLNYDVLVLAL
                     GGITNYVGVEGAEEFSKPFRKLEHADDLRALMVTALDRIPPDMSPLDMRQALTFAVVG
                     GGASGCELSTKMADLLNDAVKRRGLNGEPRVLVIEMADRIVPGMGDQIRVFVEDALRQ
                     SKVEAHTETRVVKVTQDGITFEHEGNQESLKTAAVVWTGGVKMNPVIEHLDVEKNKRG
                     LLMVKPTLQLSQYVNVFALGDIAFYPDATPTLAGTAQLAFQQASLAGSNIKLYLEGKE
                     MKTKHFEELGEALSLGTERAAVLTGGKAFGGALARQARFALYTSRLPTWHHRLRVGAS
                     WFFEGTTPQPLLPLGIER"
     gene            9135..9767
                     /locus_tag="VFH96_06100"
     CDS             9135..9767
                     /locus_tag="VFH96_06100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6973578.1"
                     /translation="MAKLRILLCVVFLVVVSLVWLTITYRTKPVLPSIDLPPTSPVAV
                     GTPLPPEPEPSPSPESANFIGQVNLIVPVAGVRPDQLIDTFDDARSEGRVHDAIDIPA
                     PAETPVLAAANGKILKLFQSERGGTTIYQLNTNGDLVFYYAHLARYADGLAEGNVVNQ
                     GQVIAYVGDTGNAGPGNYHLHFSIATISDPKRYWEGTNINPYPLLHDRTH"
     gene            9850..11145
                     /locus_tag="VFH96_06105"
     CDS             9850..11145
                     /locus_tag="VFH96_06105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET6973579.1"
                     /translation="MTLIRRLFILAWFLIVLTTGCAPAPQSNSNSTVSTQPSPSPSAS
                     PSPAITIADTASVQITLPLLDALLTDDKFVARVKKDLKLNDQQIEELKNVSGSAVARL
                     RESNAEDLDGDEPAAPARAEDQLRSVLGEEKARDLAKLANDYWSKGDASEASNDNIQM
                     LKGPNAVPTDTRVVVNIPAFRMDLFQDGSLVKSYKVGIGYPQFPLPLGLRRAQQIIFN
                     PTWTPPDSPWVAKMKNVAPGETVEAGDKDNPLGPIKIPIGLPSLIHGGKSPAKIGKFA
                     SHGCVGLTNAQIKDFATMLGQISNTPGVPTKMATYLEDETKTQTVKLTQTVPVELRYE
                     TIVIEDGKLHIYRDVYAQDTNTEENLRRVLEANGMKLEDLSEQQRTQILAALNSKPKK
                     EVVIDLGTVAQKGYPAPVDLNTGTTDKTDKQRGSKRIRT"
     gene            complement(11135..12196)
                     /gene="dusB"
                     /locus_tag="VFH96_06110"
     CDS             complement(11135..12196)
                     /gene="dusB"
                     /locus_tag="VFH96_06110"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET6973580.1"
                     /translation="MTEIKPFKIRDVEISPPLVLSPMAGVTDVSFRRLLKKRGGVGLS
                     VSEFISVEGLTRNNPKSKRQMRFYEDERPFAVQIFGGQVERMCMAAEMAQEVGADILD
                     VNCGCPAPKVVKHGGGSGLLKDHSRLADILKAIKKTITIPLTVKIRAGFYDHTINAVD
                     TAKLAEDCGVEHIALHGRTKEQGYRGLANWDLVRQVKEAVRVPVSGSGDVTTIEGAFA
                     KFRETGCDGVLIGRGAMANPWIFRQIEDAMNGRPIFEPTLADKRAILHEYFDMLREDM
                     PETPAINRMKQLAGQFTRGLQGGALFRTSIYHSHTVEEVLDRIEEYFEAIESGRPYYG
                     EGGPPINADEITDCAAFTS"
     gene            complement(12196..12723)
                     /locus_tag="VFH96_06115"
     CDS             complement(12196..12723)
                     /locus_tag="VFH96_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET6973581.1"
                     /translation="MTLFISLLICVICVHPWQGQPIEKPRIVIYKHERKLELYSNKKL
                     IRTYRVGLGFSPVADKVREGDGATPEGDFYVFVKNNKSAYYLSLGVSYPNVEDAERGL
                     RDGLINRAQYNTIVEAHKKKTAPPQYTKLGGLIYIHGNGAKSDWTWGCVALENEQMKE
                     LFDAVSVGTPVTIKP"
     gene            complement(12748..13851)
                     /locus_tag="VFH96_06120"
     CDS             complement(12748..13851)
                     /locus_tag="VFH96_06120"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6973582.1"
                     /translation="MKVKYFAAAFAVLILFSATSVFAQEGEMQVVDEVIAQVNDDVIT
                     LSMLKKETKERIEALKQNGMTEQQAMDEVSKRQNELIATLINEKLLLQRGKELELSND
                     VEAEVNRRMLEIAQQQGINSIEKLYEAMRQSGLVPEDVRRTMRNEMMKQAVLQQEVDR
                     KVYLGPSTDEVKKYYEAHPDKFRKPETVKLSEIYLVTTGKDEAAVKARALELITQARA
                     GADFGALAAANSEREKEGKRTAPQDKGYVGEFDVPSLREDLGATLKDVKAGGVTEPIR
                     TSEGYQILRVDARIPGGATPTFNDNRVREAMLMERQPKERENYLQNLRNEAFIKVTDS
                     YRAGVDPLLKIQAPVAAKGDDKKKKDEKKSNKP"
     gene            complement(13946..17569)
                     /gene="mfd"
                     /locus_tag="VFH96_06125"
     CDS             complement(13946..17569)
                     /gene="mfd"
                     /locus_tag="VFH96_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET6973583.1"
                     /translation="MIEQVTETREYKRLLAELRRGERVISISGLVAGSARALAVAALQ
                     RETGKTFAVLSHTTRDLETWESDLEFWYSALSGKEADEKDILVLPASETDPYAGISPH
                     AQTLERRALALWRLQQRSSKFVLLTARALARKTVSPAAIAKTGVVLRRDEDFSPEVLV
                     ERLIATGYMREDPVGGVGEFSLRGGIVDVWPPGREAPVRLEFFGDTVDSVREFDPENQ
                     LSTAQLKEVEVPPMREFSVNALDFQLWSEAARERWDDERYARSLRDRTAFADEGEAFA
                     GWEWLVPITMDCCSSVFDHLGDVVLVIDEPSGIEAYLSEFYERLSDRYREIDEADDIA
                     LTPEELFLTADELRELISTRQRVELRTLGRAASEIDMDLALEAEAPTVQLGRARGMRQ
                     PVFLFPAVESAVEVDWKSQSTLRYHGRIADLAAEVSRAVEEARTTLFVMPSIGVAERI
                     VEILNEYKIDTRLTYTTEASDPTSWRPVIVTVGDLSGGFEMPQARLVVHVESDVFDET
                     AAAVERRPAAVERTQRTERKKRRSKAAAFLSDFRDLKPNDYVVHIDHGVARFGGLQTL
                     DLGPRTGEFMLLYYADDAKLYVPVERLDLVQRYSSAEGHQPQLDRLGGLGWQKTKAKA
                     KRAMRDMADELLRLYAERKLVGGYAFAADTPWQQEFEDGFPFVLTPDQETAIEDVKKD
                     MEEAVPMDRLLCGDVGYGKTEVAMRAAFKSVMEGKQAAILTPTTVLAYQHYDTFRNRF
                     APFPVKVELLSRFRSTKEQKEVVKRIETGEVDVIIGTHRILSKDVQFKDLGIVVVDEE
                     QRFGVAHKERLKHLKKKVDVLTLSATPIPRTLNMSLSGMRDMSLIETPPRDRLAIQTQ
                     VVQFSESVIKSAIDLELSRGGQVFFIHNRVETIETVAALVKRLVPNARLAVGHGQMNE
                     KDMERVMLDFIDYKYDVLVATTIIENGIDIPRANTIIINRADHYGLSQLYQLRGRVGR
                     SSRRAYAYLLIPSEQELSPIARRRLAAIREFSDLGAGFRIAALDLELRGAGNLLGGEQ
                     SGHMEALGFDLYTQMLERTVAELQGEEVEDETSVTLNLGVDVAIPDDYISDMGQRLRT
                     YKRVSSARDEATLTAIREETEDRYGRIPVPVDRLFSYARLRRLSEELGIVSIDKTPDG
                     VAVKFSEKARISPEKLGDFVTKNQGATFTPNGVLRLVLTEDEQDNVLDAARAVLLQIR
                     ASD"
     gene            complement(17622..18095)
                     /gene="rfaE2"
                     /locus_tag="VFH96_06130"
     CDS             complement(17622..18095)
                     /gene="rfaE2"
                     /locus_tag="VFH96_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HET6973584.1"
                     /translation="MIEKILTRAAMLAERERLRAGGRRLVFTNGVFDLLHVGHVRYLA
                     QARALGDALVVAINSDRTVRELKGPNRPVFNQAERAEILAALRVVDYVVVFDDVSPRS
                     LIAELLPDVLVKGGDYDLDQIHGREEVEAAGGKVISLPFVEGSSTTDLIKRMGRG"
     gene            18159..19664
                     /locus_tag="VFH96_06135"
     CDS             18159..19664
                     /locus_tag="VFH96_06135"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET6973585.1"
                     /translation="MSIPFIEENDQTRARREHLEALQKLVGNVYPNKYERSRVVDVEK
                     DDTITAVVEKFTDFQPQEDLNKFVVRVAGRIATPPRQMGKVAFVHLSDGVSRLQIYIR
                     KNDVVGVYNSADGGDVDGWELFLLLDHGDFIGVEGFLFVTRTGELSIHVQKIQFLSKA
                     LLPLPDKMHGIQDPEIRQRQRYADLIAGSLKVEKDDELTAREVFEVRAAVVRELRHIL
                     EDHGYIEVETPMLTPLPTGAAARPFKTHHNALDIDLYARIAPELYLKRLLVGGFEKVY
                     ELNRNFRNEGISTRHNPEFTMLEFYWAYADVNAMMDFCEQLLRTVVFTVLGHTHVKFG
                     EHEIDFGSLFSRVGMKESLTGRVPADQIADDKVLELFEAHVEHELVQPTFITDFPKPI
                     SPLSKASPANPNVAERFELYIANMEIGNGFSELNDPEEQYERFRDQVKQRERGDEEAM
                     LMDEDYIRALSYGMPPAAGIGIGIDRLVMLLTNRTSIRDVILFPHMRPKVD"
     gene            complement(19760..>20045)
                     /locus_tag="VFH96_06140"
     CDS             complement(19760..>20045)
                     /locus_tag="VFH96_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6973586.1"
                     /translation="NLSADAVMISNSGVADKKTSLTAMEGEACEVKSYELMDLKVTFL
                     NSSTAILTYKSTQDATCGGEAVPPTVWSSSVYVMRGGRWYAASHQETIAK"
BASE COUNT         4363 a         5767 c         5381 g         4534 t
ORIGIN      
        1 cccgatcgat ggatgtattg ctcgcagtag aagtttctcg atctcacccc agatacgctc
       61 ggcaggcaga tcgctgagat cgatcgtgcg gcaaagctca atggtctccg gctcaatgtc
      121 gaactcaaac cgtgcggcaa actgcgccgc acgcagcacg cgcaaactat cttctgcaaa
      181 cgtgtctttc gagactgcac ggagtacacg ctgttcgatg tcgcgacggc cttcaaatgg
      241 atcgattagc tcgccggtga gtggatcctc gagaatagcg ttgatggtga agtcgcgacg
      301 gtgcgccgct tcggcaacgc tcatcgaggg atcgccttcg atcacgaaac ccttgtgacc
      361 gcgtcctgat ttacgttcac gacgagggat cgagacgtcg agatgattcc cgagcttgta
      421 gaccgtgaag gcttcaccaa cgacgttgac tggtccaaac tggtccagaa cctctctcag
      481 acgcgcagca tccagattgt agatttccag atcccagtcc ttcggttgca cgcccatcag
      541 cgcgtcgcga acacagccac cgacgagcaa ggcacggccg cccgcgtcgt gtattgcgcg
      601 agcaagagac aagacgttgt tattagccac ggataaacat ggatttttta cggatcttct
      661 tcaggagatt cgacgaggat ctgcacgccg cgcgtggacg gcaaacaact gatcacctgc
      721 gcgcgatcga tctttgtcat gtcgctgccg gctgcgtgcc aaagcttcac ccagaaagtt
      781 cgcgtcggtc cgtgaatcgc gaaggtcgat ccatcaatca ttacgggcac cggctcggca
      841 accgttcctg ctcgcaacgt ctccagtagc tccggaatat acggcttgaa cttctcaaac
      901 tggaccagca ctgcattgtc atcgatctct gccggcggca gctcgaccgg atcttccgac
      961 tcgagcaaaa gccgcgtctc agtttgctgg ttccgtttct taactacagg catagaattc
     1021 taaatcgaat ccgccaccgg atagggcttc aaacgcttct gcaaatgctc caccagatcg
     1081 gcaaccttga tccgctcctg ctcccacgtg tcgcggtcgc gcactgtgac tgcttcatcc
     1141 tcgagcgatt ggtgatcgac ggtgatgcaa aacggagttc cgatctcatc ctgacgcgcg
     1201 tagagttttc cgatgccgcc agtgtcgtcg taaacggcgc gcagcaccgg ttgcaacgat
     1261 cgcttgatcg ccctggccat gcccacgatc tccggtttgt tcttcgcgag cggcaagatg
     1321 gcagctttga ttggtgcgag ctgcgggctc agtttcagaa tgactctggt ctcgccctta
     1381 tcgttcgtct tttcagagta ggcatccatc aacaccgcca gcaccgttct gttgacacct
     1441 gccgccggtt cgatgacgtg cggataaaaa cgctgcttcg ccgcctcgtc gaagtacgac
     1501 agatcctcag tcgagtctgg gttgaggcgc tttccttcag cgtcgagttt attcttcgag
     1561 tggatgccca aatcgaaatc agtgcgccag gcaattccca tcaattcttc ccacggtttt
     1621 tcacgctcgg gaaaatacct gtacatgata tcgaccgtac gtttggagta atgcgccagc
     1681 tcctcgttcg gaacttcata cagctcgaga ttctcgcgat tgacaccgag ggtggcaaac
     1741 cattccaggt attgttcgac ccagtagtcg accaatttaa agtgttcgtc agttggtgaa
     1801 acgaaatact ccatctccat ctgctcgaat tcacgagttc tcaaaatgaa gtttcccggg
     1861 gtcacttcat tgcgaaaaga cttaccaacc tgcgcgatac caaatggcaa tttgcgccgc
     1921 atggtcgtct gcacattcaa aaagttgatg aatatgccct gagcggtttc aggtcgcaga
     1981 taagccaacg ccgtcggatc gtcatctgca accgcgccca atttggtttg cagcatcgtg
     2041 ttgaacggcc gcgcttcggt ccagtcgctc gcaccgcatg aaggacattt attgtcgaca
     2101 atcttgtcag cgcgaaaacg gcctttgcac ttgcggcaat cgatcagcca atcattaaac
     2161 gtcgcctcat ggcccgagta cttccaaacg agccgattga gaatgatcga cgagtcgatg
     2221 ccttcgatgt cgtcacgttc gtagacgagc cagcgccacc aggcttgttt gacgttattg
     2281 cagagctcga cgccgagtgg accatagtcc caggcgctgc cgaggccgcc ataaacttcg
     2341 gaggagggga aaacaaaacc ccgccgctta gcgagagaaa cgatggtgtc gatgtttggt
     2401 gcaggcacga atacaggatt atcaaaattc cggaaattag taaaacaagc cgcggattta
     2461 cgccgataaa cgcggatcag aattggaaat attttcagat cggcgttcat ccgcgtaaat
     2521 ccgcggctca tgttctgtct tatttacttg gtgtttgaat gttgacgtcg atcttctgcg
     2581 atcctccacc tccgaatatt cggtttctaa acataaacac tccaactaat acgatgagca
     2641 cgaaaatcac cacaatagcg acaacgccga cactaccggc accaccacca ccttcttcgg
     2701 ccatgaaggc tcctccttgt ttttgagcga gattaccgag ggggctaact ggagggaacg
     2761 gcgtaactgt aaaccagaaa gaaaaattta gccacagatt tacacggatt tgatccggat
     2821 ctgatccgtg aaatctgtgg ctaaataaaa cctaacgttt gcggcgcttg atttctcccg
     2881 cttgcaccgt gggcgtggct tgcggggcag cggcgccgct cggaactatc cacagctcga
     2941 cactgtcttc ctcgcgataa ccaccgttca caaccgagag ccgcgaagca tcgatgccgc
     3001 gctgcgtaat catatactcg cgcgcacgat tcatcagctt ctcgacctgg cccaccgggc
     3061 tcatccgtcc accataggcg atgatgtaag cacgagtagt cgggtcgttc tgcaattcga
     3121 ctgcaaggtt gtcgagacgt gccttctgat cgtcgaaagt gcagttgtta cactcgtcaa
     3181 actcacgagc tacaatcgcg accttcttga tcggcacgat cgtcgaaact gcctgcgcgc
     3241 tttgagcaca cgccggatca gacgatccat catcagcagt caacgtcgcg atgatgcgtt
     3301 gcccgccgag tccggtggac tcgatattca acgtcgacgt tcccaaaccg ctgatcacgt
     3361 gagcactgga tggcgtgact acccagttgt atttcagcgc tgcgttgcca ctgtaagcaa
     3421 cgtcggcggt gtaagtgatg atctcgcctt ccgtgacctg gtttggcgcc gagatgctga
     3481 ccgggaatgg gcacggtgac ttcggcaatg gtggcaacgg acgataattc accaccacgc
     3541 acttcacgcg tccggcgggc agctcgactt cacgtgtcca gaactgtcca ttcggcaacc
     3601 tgacctgtac ggtgtgcaat ccggcatcga gatcgaaggc acgaaaatcg gcagtggttt
     3661 ccccatgcgg cttgccatca atcatcagca ggagaccgcc cgggttcgtc tgaatcttga
     3721 tagttgcgta tttcgctttc ttcggtttgg cagacgctgt cggcgccggc accgcaaaga
     3781 agacaagcgc aaatagagcg atgaaagcaa acttgagatt acggaggata gggctacgtt
     3841 gcgacattat tggtcctcct taatcttcga tttcgcataa cgttttctca aacccgcacc
     3901 ggcagcgagc aagcccgttc cgaacaacag cagcgacgcg ggctctggag tgggcgttgg
     3961 agttgaattt ggcgtcggtg tcggtgtcgg cgtcggcgtt gggctattgc ccggtggcgg
     4021 cgtcggcgtt ggctgttcgt ctgaatcacg gtcgatgaaa acgaggggca cagccgcgag
     4081 gaacaggaac ggccacttcg ggaacgaagc accggcgata aagatctcgc cgcagtcgca
     4141 gatcgtgcct tcaacttcgc cgtcttcgat ttcctcgact ccgatcgttt tcccggcgcc
     4201 ggttactaca acactggaaa taaccgattc ggttttgccg tcggtttgtt ttggatcttt
     4261 agcctttgcg acagactcgc cttctttttt gggatcctgg gtaagcgtgt tgagcctcag
     4321 gtcgataaca ccttgagtgc tggtaagagt ttggaccacc tggttgaatt ttaccggttc
     4381 agccagggcg ggcaccgcgg tcaaaagaac gaccagcatc gcggggagca cgcgactggt
     4441 tcgcccacgt ttcgggagtg tcttacgaga gtacagggca caagttgaat tcatactgtt
     4501 tacagcgttg gacgccaacc gccttgatct aatcgagatg tctaatccaa tacagggaca
     4561 gtgctaataa ccaaaaaact ccagccgcac ctaaagcttt cgttattgcc tgcagtttgc
     4621 tgggtgaggg agcgcccagg gacaatagtc aatcacacat aaaagtgtga aaaactacga
     4681 gcaattcgag ggccgcggaa aatatctgga aaaatcgaga gatttgaggc ttagaccgca
     4741 aatctgtcta ggacctggac gcgctgtcta gaaactggac cattactgga ccaaaaaaat
     4801 aggtcctata ggacttatag gacctatcaa acttcagcac gtgttcctgg tctactcgcc
     4861 gccgccgtcg ctcgggctgg cggcttcggc tttgcgattc tcttcgcgca acacggcttt
     4921 ccgcgagagc ttgatcttgt tgccttcgcg gccgatgcac ttcaccatga tctgctgccc
     4981 ttccttcaac tcgtcacgaa cgtccttaac acgacgatcc gcgatctccg agatgtgcag
     5041 caagccgtcc gtgcccggaa agatctctac gaacgcgccg aagtcaacga tccggttgac
     5101 cgtgcccaga tacgtctggc ccacttcggc ttccgcagtt agaccacgaa tacgatttac
     5161 cgccgcatcc gcagcgtcac cacttgcggt agcaatcgaa atcgtaccat cgtccgagac
     5221 gtcgatcttc gcgcccgtct cttcgacgag cgatcggatc actttaccgc ctggtccaat
     5281 gacgtcgcga atcttgtccg ggttgatctt gatttgaata atgcgcggag cgtgtgccga
     5341 gatctcctcg cgtggctcgg ccagcgtttt ctccatcgcg ccgagaatgt agagccggcc
     5401 tttctttgcc tgctcgagcg cctcggccat gatctgcgcg ttgatgcccg cgatcttgat
     5461 gtccatctgc aacgccgtga tgccgttgcg cgttccggcc accttgaaat ccatgtcgcc
     5521 gtaatgatct tcggcgcctg cgatgtcagt cagaatcgcg tacttgtttc cttccatcac
     5581 gagacccatc gccacgcccg ccaccggagc tttcagaggc acacccgcgt ccatcaacga
     5641 aagcgtcccg ccgcaaacag tcgccattga tgacgagccg ttcgattcgg tgatatctga
     5701 aactatgcgg atggtgtatg gaaaatctga atcttcaggc aggactgtct caagtgcgcg
     5761 tcgcgcgagg gcgccgtgtc cgatctcgcg ccggctcgac gagccgtaac gtccgacttc
     5821 accgaccgaa tactgcggga aattgtagtg cagcagaaaa cggcgtttga cttcgccctt
     5881 ttcgagatcg tcgatgaact gctcgtcttc cttggtgccg agcgttgtcg aaacgagtgc
     5941 ctgcgtctcg ccgcgtgtga aaagcgccga gccgtgtacg cgtggcagcc aaccgacttc
     6001 cgaagtgatc ggacgaatct ctgaaaatct tcgtccatcg ggacgccgtc gtttgttgag
     6061 aatatcgtcg cggaagacca tctctttgag ctggtcgaag atcagtttcg ccatcgagcg
     6121 tttttcaggc tgatcttccg gatacgactc gacaacttcc ttcttcagcg cgtctaccgc
     6181 cgcgtagctg gcgcgttttt cctgagcgct cgtgtcgaga gccgtgcgca ggttttccgc
     6241 gtaacgctcg gtgatctcgc gggtcatttc gggatcgagc tccagaggtg tgacttcacg
     6301 cttctggatt tcgagcgctt tgtagagctc tctctgccag cgacagaggc gattgatctc
     6361 cttatgcgcg agcatcaggg cttcaaccat aatctcttcc gaaacttcac tcgcaccggc
     6421 ttcaaccatc acgattgctt cttcagtgcc ggcgacaatc aagttcaagc gcgactcacg
     6481 cacttcgtcg taagtcgggt taacaatgta acgtccctcg atcaacccga tacgcacgcc
     6541 cgcaatggga ttcataaacg gaatatctga tagataaagc gcgcacgacg cgcccgtgat
     6601 ggcaataacg tctggattgt tgtcggggtc ggcggaaata acgctggcaa taacctgagt
     6661 ttcgtttcga taaccttcgg caaacagcgg gcgacaggga cggtcgatca ggcggctggt
     6721 caggacttcc ttttcattag gacggccttc acgcctgaag taattgccgg ggatgcgccc
     6781 ggccgcataa tttgattcac gatactcgac cgtgagggga aagaaatcaa cgccttcgcg
     6841 gggggttaga gcgccaacgg cggcgaccag caacatcgta tcgccataac gtataacgac
     6901 agagccgtcc gcctgctttg caacgcggcc ggattcaacg gtcaactcct tatcgccaag
     6961 cttgattgat tctttgaggt atttcctttg tgacatttca ttctccttag cacgatacac
     7021 ctgaacatta atcaggtcgc aagcgcccac atagacgctc gctgttttgg caagctaact
     7081 tgctattaaa gttttctggg ccgccacgtg aagcggtgaa ggcgaaagca gcaaccaaaa
     7141 aaggcggcac gagcctcttt gaaccgtgaa gctcatgccg cctaattgct cgaagctttg
     7201 ggccgtacac gtccgcttgt tcgcagtcaa tcgccgtcac gttcggcact gccacaagct
     7261 cttcgataga cgcgggcttt aactgaacag agattcgaag ctctctctca acgtttgaat
     7321 ctaacgacgc agacccagac gattaactac ctcatggtag cgttcgatat cagtgcgctt
     7381 taagtaatcc aacagactgc gacgctgcga gaccattttc agcaggccgc gccgcgagtg
     7441 attgtctttc ttgtgggtct taaaatgctc tgtgaggtcg ttgattctct tgctcaaaag
     7501 cgccacctgg acttgtggtg acccggtgtc tgactcatgg gtacggaagt cagtaactat
     7561 ctgttccttg gtttgttttg ctaacgccac ttgcttaaat ggtcctcctc gaccgcagta
     7621 cactccgcct aaaagcggca accgctaaac ttaaaaagca agctcactgt aacatgaagc
     7681 cgtgaaaacg cacaaatgcg gagtttttag ccgcaaaaag gcacaaaatg cacaaaaaga
     7741 atttagttag gttaaagcgt ccgcctgaac tctgaactct tttttgtgct ttttgtgcct
     7801 ttttgcggcc aatttcggtg tactatcgcg gccgatggct aaagcaaaaa tcgtgattct
     7861 cggaggcggc ttcgggggta tttttactgc actcgaacta gccggagccg cggacgtgac
     7921 cctcgtcagc gacgccgatc acttcctgtt cacgcccatg ctctacgagt atctgagcgg
     7981 cgaagtcgag gcatggcaca tcgcgccaag gtacgatgaa ctcctcgaag agaacgttca
     8041 ttctgttaaa agtgcggcca ccggaatcga cctcaagtcg cagtccgttt cactcgccaa
     8101 ccagcaaact ctcaattacg acgtactcgt gctcgccctc ggaggcatca cgaattacgt
     8161 cggcgtcgaa ggcgcggaag agttttcaaa gccatttcga aaactcgagc acgcggacga
     8221 cctgcgcgcg ctgatggtga cggcgttgga tcgtattccg cctgacatgt cgccgctgga
     8281 catgcgccaa gcgctgacat ttgcagtggt cggcgggggc gcgagcggct gtgagctatc
     8341 taccaagatg gctgacctgc tgaacgatgc agtcaagcgg cgcggattga acggcgagcc
     8401 gcgcgtactc gtgatcgaga tggccgaccg gattgttccc ggcatgggcg accagatacg
     8461 cgtgttcgtt gaagatgcgt tgcgccagtc aaaggttgaa gctcatacag agacgcgcgt
     8521 ggtgaaagtg acgcaggatg gaatcacgtt tgagcatgaa ggaaatcagg aaagtttaaa
     8581 gacggcggca gtcgtgtgga ctggcggcgt gaagatgaat cccgttatcg agcacctcga
     8641 tgttgagaag aacaagcgtg gactgctaat ggtgaagccg acattgcagt tgtcgcagta
     8701 cgtgaacgtc ttcgcgctcg gtgacattgc gttttatccc gatgcgacgc cgacgctcgc
     8761 gggcacggca cagctcgcgt ttcagcaagc gagtctcgcc gggagtaaca tcaagcttta
     8821 tcttgaagga aaagagatga agacgaagca cttcgaggag cttggtgaag cgctcagtct
     8881 cgggacggag cgcgcggcgg ttttgactgg cggaaaggcg tttggtggag cccttgcgag
     8941 acaggcgaga tttgcgttgt acacgtcacg cctaccgacg tggcatcatc gattgcgtgt
     9001 cggggcgagc tggttttttg agggaacgac gccgcagcca ctgttgccgc tcggaataga
     9061 gagatagttt gaacgcggat tttcgcggat aaaacgcgga tcggcggcat agttcttgtt
     9121 gtgtagcccg cttcgtggca aagcttcgga tactgttgtg tgttgttttt ctggtggtgg
     9181 tgtcgctggt gtggttgacg attacttacc ggacgaagcc tgtgcttccc tccatcgatc
     9241 tgccaccgac tagtcccgtc gcggttggca cgccgctgcc gcccgaaccc gaaccaagtc
     9301 catcaccaga gtcagcaaac tttatcggtc aggtaaacct gatcgttccc gttgccggcg
     9361 tccgtccgga tcaacttatc gacaccttcg atgatgcgcg ctcggaaggg cgcgtccacg
     9421 acgcgatcga tataccggct ccggctgaga caccggtgct tgcggcggca aatggcaaga
     9481 ttctcaaact ttttcagagc gaacgcggcg gcacgacgat ctatcagcta aacacaaacg
     9541 gtgatctcgt tttctactac gctcacctcg cgcgctacgc tgatggcctc gccgaaggaa
     9601 acgtcgtgaa ccagggccaa gtgatcgctt atgtcggcga caccggcaat gcaggccccg
     9661 gaaactatca tctgcacttc tcgatcgcca ccatttccga cccgaaacgc tactgggaag
     9721 gcaccaatat caacccgtac ccactgctac acgaccgaac gcactagccg gcctatcgtt
     9781 cgcaaatcgt tcagaaaatc cgtgtaaata gtgtctcccc actggaacgc ccgttgctca
     9841 agcactcgca tgaccttaat tcgtcgactt tttattctcg cgtggttcct gattgtactg
     9901 acgacgggct gtgcgcccgc gccgcagagt aattcaaact caacggtgtc gacacagccg
     9961 agcccatctc caagcgcatc gccgtcaccg gcaatcacca tcgccgacac cgcgtcagtc
    10021 cagataacac tgccgctact cgatgcactg ttgaccgacg ataaatttgt ggcgcgagtc
    10081 aaaaaggatc tcaaactcaa cgaccagcag atcgaagagc tcaagaacgt ctctggatcg
    10141 gcagtggcgc ggctgcgcga aagtaacgct gaagatctcg acggtgatga acctgctgca
    10201 ccagcgcgcg ccgaagacca gttgcgctcg gtgctcggtg aagaaaaggc acgagacctc
    10261 gcgaaactcg caaacgacta ctggtccaaa ggcgacgcct cggaagcttc gaatgacaat
    10321 atccagatgt tgaagggacc aaacgccgtg ccgactgaca cgcgtgtggt cgtcaacatc
    10381 cccgcgtttc gcatggatct ttttcaggat ggatcgctcg tgaagtcgta caaggtcggc
    10441 atcggttatc cgcagtttcc tttgccgctc ggattaagaa gagcgcagca gatcatcttc
    10501 aatccgacgt ggacgccgcc ggattcgcct tgggtcgcga agatgaaaaa cgtcgctccg
    10561 ggtgagactg tcgaagccgg cgacaaagac aatccgcttg gtccaatcaa gataccgatc
    10621 ggactgccgt cgctgattca tggtggcaag tcgcccgcga agatcggaaa gtttgcctcc
    10681 cacgggtgtg tgggactaac gaacgcgcag atcaaagact tcgccacgat gctcgggcaa
    10741 atttcaaaca cgccgggcgt gccgacgaag atggccacgt atttagagga cgagacgaaa
    10801 acgcagactg taaaactcac ccagaccgtg ccggtcgagt tgcgctacga gacaatcgtg
    10861 atcgaggacg gaaagctaca catttaccga gacgtttacg cgcaggacac caacaccgaa
    10921 gagaatctgc ggcgcgtgct cgaagcgaat ggcatgaagt tggaggatct ttcggagcag
    10981 cagcgaacgc agattctagc tgcgttgaat tcgaagccga agaaagaggt cgtgatcgat
    11041 ctgggaacgg tggcgcagaa gggttatccg gcaccggttg atctgaacac agggaccacg
    11101 gataaaacgg ataaacagcg cggatcaaaa cggatcagga cgtgaaggcg gcacaatccg
    11161 tgatttcatc cgcgttgatc ggtggcccgc cttctccgta atatgggcgg ccggattcaa
    11221 tcgcttcgaa atactcttcg atgcggtcca atacctcttc aactgtgtgg gagtggtaaa
    11281 tcgaagttcg gaagagcgcg ccgccctgca agccgcgcgt gaactgaccc gccaactgct
    11341 tcatccgatt gatcgcgggc gtctccggca tgtcttcacg caacatgtcg aaatactcgt
    11401 gcaggatcgc gcgtttatca gctaacgtcg gctcgaagat cggacggccg ttcatcgcgt
    11461 cttcgatctg ccggaagatc cacggattcg ccatcgcgcc acggccaatc aacacaccgt
    11521 cacaaccagt ttcacgaaac ttcgcaaacg cgccttcaat cgtcgtgacg tcaccgctgc
    11581 cagacaccgg cacacgcacc gcttccttca cttgcctgac gaggtcccag ttcgcgagac
    11641 cacgataacc ctgctctttc gtgcgaccgt gcaaggcgat gtgctcaacg ccgcaatcct
    11701 cggccagctt cgcagtgtcc accgcattga tcgtgtgatc gtagaagccc gcccgaatct
    11761 tcaccgttaa cggaatcgtg atcgtctttt tgatcgcttt gagaatgtct gcgagacggc
    11821 tgtgatcctt gagcagcccc gatccgccgc cgtgcttcac gactttcgga gccgggcagc
    11881 cgcagttcac gtcgagaatg tcggcgccga cttcctgcgc catttccgcc gccatgcaca
    11941 tgcgctcaac ctggccacca aagatctgca ccgcaaaggg gcgctcgtct tcgtagaagc
    12001 gcatctgtcg tttcgacttc ggattgtttc tcgtcaaacc ctcaactgaa atgaactcag
    12061 aaactgacag accaacaccg ccacgctttt tcagcagacg acgaaaagag acgtccgtca
    12121 cgcccgccat cggtgacaaa accagcggcg ggctgatctc gacgtctcga attttgaaag
    12181 gcttgatttc cgtcatcacg gtttgatcgt caccggtgtt ccgactgaaa cagcgtcgaa
    12241 caactccttc atctgctcat tctccaacgc gacgcagccc catgtccaat cgctcttcgc
    12301 gccattcccg tgaatgtaga tcaggccgcc gagttttgtg tactgcggcg gcgcggtctt
    12361 cttcttgtgc gcttcgacaa tcgtgttgta ctgggcgcgg ttgatcagac cgtcacgcaa
    12421 gccgcgttct gcgtcttcga cgttcggata actgacgccg agcgacagat agtaagcgct
    12481 cttgttgttc tttacgaaga cgtaaaagtc accttcggga gtcgcgccgt cgccttcgcg
    12541 caccttgtcg gcaaccggac taaacccgag cccgacgcga taggtgcgaa tcagtttttt
    12601 gtttgagtaa agctcgagct tacgttcgtg tttgtaaatg acgatgcgag gcttttcgat
    12661 gggctgacct tgccacggat gaacacagat aacacagatc agaagtgaaa tgaacagtgt
    12721 catccgtgtt catccgtggc tagaaactta tggtttattt gatttcttct cgtccttctt
    12781 ctttttatcg tcacccttcg cggccactgg cgcctggatc ttcaggagcg gatcaacgcc
    12841 ggcacggtat gagtcagtga ctttgataaa cgcttcgttt cgcaggttct gaagatagtt
    12901 ctcgcgctct ttcggctgtc gctccatcaa catcgcttcg cgaacgcgat tgtcgttgaa
    12961 cgtcggcgtt gcgccgccag ggatgcgggc gtcgacacgc aggatctgat aaccctcgct
    13021 tgttctaatc ggctcggtga caccgccggc tttcacatcc ttcaacgtag ccccaagatc
    13081 ctcgcgcagg ctcgggacgt caaactcgcc gacgtagccc ttatcctgcg gcgctgtgcg
    13141 cttcccttcc ttctcacgct ccgaattcgc tgcggcaagc gcgccgaaat ccgcacccgc
    13201 gcgggcctgt gtgatcagct cgagcgcgcg agccttgact gccgcttcat ctttgccagt
    13261 cgtcacgaga tagatctcgg aaagcttcac ggtctcgggt ttccgaaact tgtcaggatg
    13321 agcttcataa tacttcttta cttcatcagt ggacgggccg aggtaaactt tccggtcaac
    13381 ttcctgttgc agcaccgcct gcttcatcat ctcgttgcgc attgtgcgac gaacgtcttc
    13441 gggaacgagt ccgctctggc gcatcgcttc gtagagcttt tcgatcgagt tgataccctg
    13501 ctgttgcgcg atttccaaca tacggcgatt aacttcagcc tcgacgtcgt tggagagctc
    13561 gagctccttg cctctttgca gcaacagctt ctcgttgatc aacgtcgcga tcagctcgtt
    13621 ctggcgcttc gagacttcat ccatcgcctg ctgctcggtc atgccgttct gtttcagcgc
    13681 ttcgatgcgt tctttggttt ccttctttag catcgagagg gtgatcacgt cgtcgttgac
    13741 ctgggcgatc acttcgtcga cgacctgcat ctcgccttcc tgtgcgaaga cgctggtggc
    13801 gctgaagaga atcagcacgg caaaggctgc tgcaaaatac ttaactttca caataaaaac
    13861 tcctggttat tgttatccgc agattgcaca gattacacag attacacaga atgaatctgt
    13921 gtaatctgtg gataagtttt ggattctaat cgctcgcacg aatctggagc aacacggcgc
    13981 gggctgcgtc gagcacgttg tcctgctcgt cctctgttaa aaccagtcgc aaaacaccgt
    14041 tgggcgtaaa cgttgctccc tggttcttcg tcacgaaatc tcccaatttt tcgggtgaaa
    14101 tcctcgcctt ttcactaaat ttgaccgcca ctccgtccgg cgtcttatcg atcgaaacta
    14161 tccccaactc ctccgataaa cgacgcaaac gtgcatagga aaatagcctg tcgaccggca
    14221 ccggaatacg tccatagcgg tcttccgtct cctccctgat tgccgtgagt gtcgcttcgt
    14281 cacgggccga agaaactcgt ttgtacgtcc gcagccgctg tcccatatcg gagatgtaat
    14341 cgtccggaat ggcgacatcc acgcccagat tcagcgtgac gctcgtctca tcctcgactt
    14401 cttcaccctg caactcggcg acagtgcgct cgagcatttg cgtgtaaaga tcgaagccca
    14461 gcgcctccat gtgccccgac tgctcgccgc cgagcaggtt gcccgcgcca cgtagctcaa
    14521 ggtcgagggc ggcaatacga aacccggccc ccagatcaga aaactcgcga atggctgcta
    14581 aacgtcgacg ggcaatgggc gaaagctcct gttcactcgg tatcagcagg taagcataag
    14641 ccctgcggct ggagcgcccc acacgcccgc gtaattgata aagctgagaa agaccgtagt
    14701 gatcggcgcg atttatgatg atggtgtttg cacgcgggat gtctatgccg ttctcgatga
    14761 tggtggtcgc aaccagaacg tcatatttgt agtcgatgaa gtcgagcatc acccgctcca
    14821 tgtccttttc gttcatctgc ccgtggccca cggcgagacg cgcgttcggc acgaggcgct
    14881 tcacgagggc cgcgacggtt tcgatcgtct ccacgcgatt gtgaatgaaa aagacctgcc
    14941 cgccgcgtga gagctcgaga tcgatcgccg acttgatcac actctccgaa aactgcacca
    15001 cctgcgtttg aatcgcgagt cgatcgcgcg gcggcgtttc gatgagcgac atatcgcgca
    15061 tgccgctgag tgacatattg agcgtgcgtg gaatcggcgt ggctgaaagc gtcaacacgt
    15121 cgactttctt cttgagatgt ttcagtcgtt ctttgtgggc gacgccgaaa cgctgctcct
    15181 cgtcaaccac cacgataccg agatctttga actgcacgtc tttcgagaga atgcgatgcg
    15241 tgccgatgat cacatctact tcaccggtct cgatccgctt cacgacttcc ttctgttcct
    15301 tcgtcgagcg aaagcgcgac aacaactcga ccttcaccgg aaacggcgcg aaacgattac
    15361 gaaaggtgtc gtagtgttga taagcgagta cggtcgtggg cgtgagtatc gctgcctgtt
    15421 ttccttccat cactgacttg aacgccgctc gcatcgcgac ttccgttttg ccgtagccga
    15481 cgtcaccgca cagcagacgg tccatcggca cggcctcttc catgtctttc ttcacgtctt
    15541 cgatcgctgt ctcctggtca ggcgtgagta cgaacggaaa gccgtcttca aactcctgct
    15601 gccagggtgt gtcggcagcg aaggcgtagc caccgacgag ttttcgttca gcgtaaagac
    15661 gcaaaagctc gtcggccatg tcgcgcatcg cgcgctttgc tttggctttt gtcttttgcc
    15721 agccgagacc gccgagtcga tcgagctggg gttgatgtcc ttccgcgctc gaataacgct
    15781 gcacgaggtc gagacgctcg acgggaacgt agagcttcgc gtcgtcggcg tagtaaagca
    15841 gcatgaactc gccggtgcgt ggtccaagat cgagcgtctg caaaccgccg aagcgcgcga
    15901 cgccgtgatc gatgtggacc acgtaatcgt tgggcttgag atcgcgaaag tcggaaagga
    15961 aggccgctgc tttcgacctg cgttttttcc tttcggttcg ctgtgttctt tcaacggctg
    16021 cgggtcgtcg ctcgacggcg gcggcagttt cgtcaaagac gtctgactcg acgtgaacaa
    16081 ccaggcgggc ctgcggcatt tcgaagccac cgctcagatc gcccaccgtc acgatcacag
    16141 gtcgccaact tgttggatca gaggcttcgg tggtgtaggt cagacgagtg tcgatcttgt
    16201 actcgttgag gatctcgacg attctctcgg ccacgccgat actcggcatc acgaagagtg
    16261 ttgtgcgtgc ttcttccacc gcgcgactca cttcagccgc gagatccgcg atgcgaccgt
    16321 gataacgaag cgtcgattgc gacttccaat cgacttccac cgcgctctcc actgccggaa
    16381 acagaaatac cggctgccgc atgccgcgtg cacgaccgag ttgaaccgtg ggcgcttcgg
    16441 cttcgagcgc gaggtccatg tctatttctg aagcagcgcg gccgagtgtg cgcagctcga
    16501 ctcgctggcg agttgaaatc agctctctta attcgtcggc ggtaagaaat agttcttcag
    16561 gcgtgagtgc gatgtcgtcg gcttcgtcga tctcgcgata gcgatctgat agtcgctcgt
    16621 aaaactcact cagatacgct tcaattccgg atggttcatc aatcacgaga acgacatcgc
    16681 cgagatgatc gaacacactc gagcagcagt ccatcgtgat cgggaccagc cattcccagc
    16741 cggcaaacgc ttcgccttcg tcagcgaaag ctgttcgatc gcggagcgag cgcgcgtaac
    16801 gctcgtcgtc ccagcgctcg cgcgccgcct cgctccaaag ttggaaatcg agggcgttca
    16861 cggagaactc gcgcatcggc ggcacttcga cttctttcaa ctgcgcggtg gagagctggt
    16921 tttccggatc gaattcgcgg actgaatcga ctgtatcgcc gaaaaattcg agtcgtaccg
    16981 gcgcttctcg tccgggcggc caaacatcga cgatgccgcc gcgtagcgag aactcgccga
    17041 cgccgccaac cggatcttca cgcatgtagc cggtcgcgat cagtcgctcg acaagcactt
    17101 ccggcgagaa gtcttcatcg cgtcgcaata cgacgcctgt cttcgcgatc gcggccggac
    17161 tgaccgtctt acgcgccagg gcacgcgccg tcagtagcac gaacttcgac gagcgctgtt
    17221 gtagacgcca gagcgcgagt gcgcgtcgct cgagtgtttg cgcgtgcggt gagataccgg
    17281 cgtatggatc agtctccgac gcgggcaaaa cgagtatgtc tttctcatcc gcctctttcc
    17341 cactgagcgc cgaataccaa aactccagat cgctctccca cgtctcgagg tcacgcgtcg
    17401 tatgcgacaa aacggcgaaa gtcttccccg tctctcgttg caacgcagcc accgccagcg
    17461 cccgcgccga ccccgccacc aacccactga tcgaaatcac acgttcccca cgccgcaact
    17521 ccgccagcag ccgcttgtat tccctcgtct ctgtaacttg ttcgatcatt tatttagccg
    17581 cggattttcg cggataaacg ctgatctgcg ttcatccgcg ttcatccgcg gcccattctc
    17641 ttgattaaat cggtcgtcga agatccctct acgaacggta aagatattac ttttccaccc
    17701 gcggcttcga cttcctcgcg cccgtgaatc tgatcgagat cgtagtcgcc gcccttcacc
    17761 agcacatccg gcagcagttc tgcgatcaga ctgcgcggtg atacgtcgtc aaacacaact
    17821 acgtaatcaa ccacacgcaa agcagcgagt atctcagccc gctccgcctg attgaaaacg
    17881 ggccgatttg gtcccttcaa ctcccgcaca gttctatcac tattgatcgc cacgacgagc
    17941 gcgtcaccca gcgcgcgcgc ctgcgccagg tagcgcacgt gcccgacgtg caaaaggtca
    18001 aacacaccat tcgtaaaaac gagccgccgg ccaccggcac gcagacgctc gcgctcggca
    18061 agcatcgccg cccgggtcaa aatcttctcg atcattgaac atcagattac aggatttgcg
    18121 ggatgtctgt ataatcgggg ctttttggag aactacacgt gtctatcccc tttatcgaag
    18181 agaatgacca aacgcgcgcg cggcgcgaac acctcgaagc attgcaaaag ctcgtcggca
    18241 atgtttaccc gaacaaatac gagcgcagcc gtgtcgtcga cgtggaaaaa gacgacacga
    18301 tcaccgccgt ggtcgagaag tttaccgact tccaacctca ggaagatctg aacaagtttg
    18361 tcgtgcgcgt cgccggacgc atcgcaacgc caccgcgcca gatgggcaag gtcgcgttcg
    18421 ttcatctttc ggatggcgtg tcgcgcctgc aaatctatat tcgcaagaac gacgtcgttg
    18481 gcgtttacaa cagcgctgac ggcggcgacg ttgatggctg ggaattgttc ctactgttgg
    18541 accacggcga cttcatcggc gtcgaaggct tcctcttcgt gacgcgcaca ggcgagctct
    18601 cgatccacgt gcagaagatt cagtttctgt cgaaagcact cctgccgctg ccagacaaga
    18661 tgcacggcat ccaggatccg gagattcgcc agcggcaacg ctatgcggat ctgatcgccg
    18721 gaagtctcaa agtggagaaa gacgacgaac tcacggcgcg tgaagtattc gaggttcgtg
    18781 ccgccgtggt gcgtgagctg cggcacattc tcgaagacca cggttacatc gaagtcgaga
    18841 caccgatgct gacgccgctc ccgaccggtg ccgcggcgcg tccgttcaag actcatcaca
    18901 acgcgctcga tatcgatctc tacgcgcgca tcgcgccgga gttgtatttg aagcgcctgc
    18961 tcgtcggcgg cttcgaaaag gtttacgagc tcaatcgaaa cttccgcaac gaaggcatct
    19021 cgacgcgtca caatccggag ttcacgatgc tcgagtttta ctgggcctac gccgacgtga
    19081 acgcgatgat ggatttttgc gagcagttgt tgcgcacggt ggtgttcacc gtgttgggcc
    19141 acacgcacgt gaagtttggc gagcatgaga tcgatttcgg atcgcttttc tcgcgggtgg
    19201 ggatgaagga gagtttgacc ggacgagtgc cggcggatca gatcgcggac gacaaggtgc
    19261 tggagctctt cgaagcgcac gtcgagcacg agctggtgca gccgactttc atcactgact
    19321 ttccgaagcc gatctcgcca ctctcgaagg cgtcgccggc gaatccaaac gtggctgagc
    19381 gttttgagtt gtacatcgcg aacatggaga tcggtaacgg gttttctgag ttgaacgatc
    19441 ccgaagagca gtatgagcgc ttccgcgatc aggtgaagca gcgtgagcga ggcgacgaag
    19501 aggcgatgct gatggatgaa gactacatcc gcgcgttgtc ttacgggatg ccgcccgcgg
    19561 cggggatcgg tatcggtatc gatcgactcg tgatgctgct cacgaaccgg acatcaatac
    19621 gcgacgtcat cctcttcccg cacatgcgcc ccaaagtaga ctaagatcac aaaaggcaca
    19681 aagaagacca caaaaagcac aaaacctttt tgggaaattc tttgtgcttt ttgtggtctt
    19741 ctttgtgcct tttgtgatct tacttggcga tggtttcctg gtgggaagcc gcgtaccagc
    19801 gtccgcctct cattacgtaa acactactgg accagaccgt gggtgggact gcctcgccac
    19861 cacacgtcgc atcctgtgtc gatttgtaag tcagaatcgc ggtactgctg ttgagaaatg
    19921 tgactttgag atccatcagc tcgtaagact tgacttcgca agcctcgcct tccattgctg
    19981 tgagcgaagt cttcttgtca gccacgccac tgttgctgat catgacggcg tcagccgaga
    20041 gattg
//
LOCUS       DASSFQ010000175         5057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_975604, whole genome shotgun sequence.
ACCESSION   DASSFQ010000175 DASSFQ010000000
VERSION     DASSFQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975604"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="VFH96_06145"
     CDS             complement(<1..1711)
                     /locus_tag="VFH96_06145"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET6973587.1"
                     /translation="MRVSRLKTSFLFSFFLLTFLFITIPVRADDDDNDDSDDYDVKAR
                     VARISLIGGEVNLRRAGNKDWETARVNYPLVEGDTITTGKGSQLEIQIDSRNFVRLTD
                     SAMLRIVTLRDEGVAISLIEGTLVLRLSKFDREKEYFEIDAPRSTLAAEKKGLYRIDA
                     PREGRVRLTVRDGGQARIYSDTSGFTLRDERTAELIASGPNAGDWDFYPASEAPDAVD
                     DWVKQREQYFASRQNRDPKYFDEYVFGAEDLDDYGDWVNANDYGWIWRPHASSLSVYS
                     DWAPYRYGQWTWCPPYGWTWVGYESWGWAPYHYGRWVYYNGYWAWCPRSQFYRHRSWW
                     RPALVAFVFDFSFGNNICWYPLNYYQQDPHSRHYRHDNRYPHPGYGNNQPGRPNEPGR
                     SEYKQWRGVTRVPRGDFGNPNRRAEGVDETVARRVLDREPDRENLPGRTFSDGRPGRQ
                     VPVIDVPDRPTGAGDRTPGVTLDEELKRRRVYRGRDPRPNEPRTPSQPGNSASPVTET
                     QPTGAVSRPQNPSTSNQPGEQPDQPGRFVRPTLPEGVLKRQRGQQAIENQPPRNNPEP
                     RSEP"
     gene            2008..3177
                     /locus_tag="VFH96_06150"
     CDS             2008..3177
                     /locus_tag="VFH96_06150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET6973588.1"
                     /translation="MVRASRYDVVIIGAGINGAGIARDAAMRGLKVLLIEKGEIGAGT
                     TSASTRLIHGGLRYLEHFEFGLVYESLRERETLLRIAPELVRPLAMAIPIYKQSRRGW
                     VTIRAGMILYDLLSWRKSLPRHQMLSREETLKRWPGLNPEGLVGSALYYDAQVEFPER
                     LVLANVQSAREFGAEVLTHTRVTSLTGSGVEFGGHFIEADVVINAAGPWIDLVLEQAP
                     VKSSKLIGGTKGCHIVVPVFPGAPANAIYLEARSDGRPIFIIPWNKLYLIGTTDVRFE
                     CDPDEVRCEDWEIDYLLSETNLALPGAKLTRDSILHTYSGVRPLPYQSSTRRHFIREH
                     PLPNLLSIVGGKLTTYRSLSEECVDLIFRKLGRDSPPCRTAKEYLATDDTDKIRI"
     gene            complement(3174..3746)
                     /locus_tag="VFH96_06155"
     CDS             complement(3174..3746)
                     /locus_tag="VFH96_06155"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HET6973589.1"
                     /translation="MFLIREPSEADAARFVASQRDLPFTYAEVGATNATPPTSYTVDH
                     NRIQIGAGAATYRKAVDALKNWRHFELGWVTIAPRGVPVEVGATVAVKARAFGTWSLS
                     ATSIVYIIDEDRRFGFAYGTLPDHVEKGEERFLIEWLPDDTVWYDILAFSRPHHPLVR
                     LSAPLARRLQKQFARASLTQMKLLATDNTD"
     gene            complement(3750..4646)
                     /gene="xerD"
                     /locus_tag="VFH96_06160"
     CDS             complement(3750..4646)
                     /gene="xerD"
                     /locus_tag="VFH96_06160"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET6973590.1"
                     /translation="MLPVERDFLREFITYLQVEKGLARHSIDGYRRDLDRLQTWSNKN
                     GKQVSELTRADLRKWIASLSREGLAPASISRAVSATRGFFKFLMLDGHIKSHPAEDLD
                     TPQRFAYLPKFLTEDEINRLFTAPDISTEEGVRDRAVLEIMYATGLRVSELVNLKHAD
                     VDLLAGLVKCHGKGNKERRVPLGKSAIHWLQQYSAVKAGYGKPSTPNMFLHRGRPFTR
                     QLAWSMIKSHAEKVGIKNVSPHTLRHSFATHLLQHGADSRSVQALLGHSDISTTQIYT
                     HITDMHLRAAYDRHHPRARMKS"
     gene            complement(4716..>5057)
                     /locus_tag="VFH96_06165"
     CDS             complement(4716..>5057)
                     /locus_tag="VFH96_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6973591.1"
                     /translation="GGIGVMNIMLVSVTERTREIGVRKAIGARRRDIVRQFLFEAMML
                     TFLGGVLGVVLAAGIAQLLMLLIPSMPASIPTWAVVTGLTVSIGVGLIFGVWPARKAS
                     RLDPIEALRYE"
BASE COUNT         1067 a         1385 c         1452 g         1153 t
ORIGIN      
        1 gtggctcgct gcgtggctcg ggattgttgc gcggcggctg gttttctatc gcctgctggc
       61 cacgctgtcg tttcaaaaca ccttccggca atgttggtcg cacaaaacga cccggctgat
      121 ccggctgttc tccaggctgg tttgacgtag acggattctg gggccggcta acagcaccag
      181 tcggttgcgt ttcggtaact ggagacgcgc tgttgcctgg ttgtgacggc gttcttggct
      241 cgtttggtcg cggatcgcgg ccacggtaaa cgcgtctacg cttcagctct tcatccaacg
      301 tcacgccggg tgtgcgatcc cctgctccgg tcggacgatc cggcacgtcg atcaccggca
      361 cctgacgtcc gggacgacca tcggagaaag tgcgacccgg gagattttca cgatccggct
      421 cgcggtcgag cactctccgc gctacggttt catcaactcc ttctgcgcgc cggttgggat
      481 tgccaaaatc gccgcggggc acacgcgtca cgccgcgcca ttgcttgtat tccgaacggc
      541 caggctcatt tggtcgtccg ggttggttat taccgtaccc cgggtgcggg taacgattgt
      601 cgtgacgata gtgtcgcgaa tgcggatctt gctggtagta attcagcggg taccagcaaa
      661 tgttattgcc aaacgagaag tcgaagacaa acgcgaccag cgcgggccgc caccagcttc
      721 ggtgacggta gaactggctg cgcgggcacc acgcccagta gccgttgtaa tagacccagc
      781 ggccgtagtg atacggcgcc cagccccacg actcataacc gacccacgtc cagccgtatg
      841 gcgggcacca ggtccactgc ccgtagcgat acggcgccca atcgctgtag acgctgagcg
      901 agctcgcgtg tggccgccag atccagccgt agtcgttggc gttgacccag tcgccgtagt
      961 catcgagatc ttcggcgcca aagacgtatt cgtcgaaata tttcgggtca cggttttgac
     1021 gcgacgcgaa atactgctcg cgctgtttca cccaatcatc aaccgcgtcc ggcgcttcag
     1081 aagctggata gaaatcccag tcacctgcat ttgggccgct ggcgattaac tcagctgtgc
     1141 gctcgtcgcg cagggtgaat ccggaagtat ctgaataaat gcgagcctgc ccaccgtcgc
     1201 gcacagtcaa acgaacgcgc ccctcgcgcg gtgcgtcgat gcggtaaagt cctttctttt
     1261 cggcggcaag ggtggatcgt ggcgcatcaa tctcgaaata ctctttttcg cggtcaaact
     1321 ttgacagtcg cagtacgagc gtgccctcga tgagactgat cgcgactcct tcatcacgca
     1381 acgtgacgat tcgcagcatc gcgctgtctg tcaggcgcac gaagtttcgc gagtcgatct
     1441 ggatttcgag ctggctaccc ttgccggtcg tgatggtgtc gccctcgacc agcgggtagt
     1501 tcacgcgggc tgtttcccaa tccttgttgc cggcgcgtct gaggttgacc tcgccgccga
     1561 tcaggctgat acgcgcgacg cgggctttca cgtcgtaatc gtcgctgtca tcgttatcgt
     1621 cgtcgtcggc gcggaccgga attgttatga ataaaaaagt gagaagaaaa aaagaaaaga
     1681 gaaagctggt ttttagtcgg gagactcgca ttgttatgct ccttcttggt tcgaagtgcg
     1741 gttgggccgg aaccgagcaa agtgcatgcc cgcgcgcacg cttttaagga gcaatgaaac
     1801 ggaattgaga attctcaatc cggtaaatcc tgtgaatatg gcaatcctgt ctaagtcttc
     1861 cacaagattg cacggttcac aggatttacc agatggatcc atcgctcgct tgaggattct
     1921 caaaatttta tttggaccag aaaccaagcg attgatcgaa ttcgatttga agtgacttta
     1981 cctgagacag tgcatgcctg acacgatatg gtgcgagctt ctcgttacga cgtggtgatc
     2041 atcggggccg gaatcaacgg cgccgggatc gctcgcgacg ctgcgatgcg cggtttgaaa
     2101 gtgttgttga tcgagaaagg tgagatcggc gccgggacga cgagcgcgtc gacgcggctc
     2161 attcacggcg gcttgcgcta tctcgaacat tttgagtttg gactggtgta tgagtcgttg
     2221 cgtgagcgtg agacgttatt gcggatcgct cctgagctcg tgcgaccgct ggcgatggcg
     2281 atcccgattt acaaacagag cagacgcggg tgggtgacga ttcgggccgg gatgattctt
     2341 tatgatctgt tgtcgtggag gaagtcgttg ccgcgacatc aaatgttgtc gcgcgaagag
     2401 actcttaagc gctggccggg actcaatccg gaaggtttgg tcgggtcagc gctctattac
     2461 gacgcgcaag tcgagtttcc cgaacgactt gtgctcgcga atgtacagtc ggcgcgcgag
     2521 tttggcgcgg aggtgctaac gcacacgcgc gtgacgagtt tgactggttc gggtgttgag
     2581 tttggagggc atttcattga ggcggatgtg gttatcaatg ctgccgggcc gtggatcgat
     2641 ctagttctcg aacaagcacc ggtcaagagt tcgaaattga ttggtgggac gaagggctgt
     2701 catatcgtcg taccggtatt tccgggtgcg cctgcgaatg cgatttacct agaggcacgc
     2761 agcgatgggc ggccaatctt catcattccg tggaacaagc tttatctgat tggaaccacc
     2821 gacgttcgct ttgaatgcga cccggatgag gtccggtgcg aagattggga aatcgactac
     2881 ttgctttctg agactaatct cgctttacct ggcgcgaagc tcacgcgcga tagcattctg
     2941 cacacgtatt ccggcgtgcg gccactacct taccaaagca gcacgcggcg gcacttcatc
     3001 cgcgaacatc cgcttccgaa tctgctctcg atcgtcggcg ggaaactcac gacgtatcgc
     3061 agcctctcgg aagagtgcgt cgatctgatc tttcgaaagc tcgggcgtga ttctccgccg
     3121 tgtcgaactg caaaggaata tttagccacg gatgacacgg ataaaatccg gatttaatcc
     3181 gtgttatccg tggccaataa tttcatttga gttaaggagg cgcgggcgaa ttgtttttgt
     3241 aatcgtcgtg cgagcggagc gctgagtctg acgagtggat gatgaggacg ggagaaggcg
     3301 aggatgtcgt accagacggt gtcgtctgga agccactcaa ttaaaaatcg ctcttcgccc
     3361 ttttcgacgt gatcgggcaa cgtaccgtac gcaaaaccga aacgacggtc ttcatcaatt
     3421 atgtaaacaa tgctcgtggc gctgagtgac cacgttccaa atgcacgcgc cttcaccgcg
     3481 acagtcgctc cgacctcgac cggaacgcca cgcggcgcaa tcgttaccca gccgagttcg
     3541 aagtgtcgcc agttcttcaa cgcatcaaca gccttgcgat aggtcgctgc accggcgccg
     3601 atctgaatgc gattgtgatc cacagtgtag cttgtcggtg gcgtagcatt agtcgcgccg
     3661 acttccgcat atgtaaacgg taaatcgcgt tgcgacgcga caaaccgcgc ggcgtctgct
     3721 tcactcggct ctcgtatcag aaacattttt tacgatttca tccgcgcccg aggatggtga
     3781 cggtcatacg ccgctcgcaa atgcatgtcg gtgatgtgtg tgtagatttg tgtggttgag
     3841 atgtcgctgt gaccgagcag cgcctgcacc gaacgcgagt ctgcgccgtg ttgcaaaaga
     3901 tgagtcgcga agctgtgtcg cagtgtgtgt ggcgaaacgt ttttgattcc cactttctcc
     3961 gcgtgactct tgatcatgga ccacgccagc tggcgcgtaa acggccgccc gcgatgcaaa
     4021 aacatattcg gcgtcgacgg tttgccgtat cccgctttta ctgccgagta ttgttgcagc
     4081 cagtgaatcg cgctcttgcc gaggggcacg cggcgctcct tatttccctt gccatgacac
     4141 ttaaccagac ccgccagcag gtcgacatcc gcatgcttca agttcacgag ctcagagacg
     4201 cgcagaccgg tggcatacat gatttccagc accgcacgat cgcgtacgcc ttcctcggtt
     4261 gagatatcag gcgccgtaaa caagcgattg atctcgtctt cggtgagaaa cttcgggaga
     4321 taggcgaagc gttgcggtgt gtcgagatct tcagcgggat gcgacttgat gtgaccgtcg
     4381 agcatcagga acttaaagaa cccgcgggtg gcactgaccg cacgcgagat cgacgccggc
     4441 gcgagacctt cgcgtgacag actcgcgatc cacttgcgca gatccgcgcg cgtgagctca
     4501 gaaacttgtt tgccgttttt gttggaccag gtttggagtc gatcgagatc gcgtcgatag
     4561 ccatcaatag aatgacgcgc gagacctttc tcgacttgga ggtaagtaat aaattctctt
     4621 aggaagtcgc gctccacagg aagcattcta catgattaca ggattttcaa aattaacaag
     4681 atgaatcttg aaaatcctgt aatcttgtct acgacttatt cgtaacggag cgcttcgatc
     4741 ggatcgagcc gtgacgcctt cctcgcgggc catacgccga agatcaatcc aacaccaatc
     4801 gatacagtca gcccggtaac aactgcccac gtaggaatcg acgccggcat actcggtatc
     4861 agcaacatga gcagttgcgc tattcccgct gccagcacca cgccgagcac accaccaaga
     4921 aacgtcagca tcatcgcttc aaacagaaat tgccgcacga tgtcacgacg ccgcgcacca
     4981 atcgctttgc gcacaccgat ctctcgcgtt cgctcggtaa cactgacgag catgatattc
     5041 atcacgccaa tgccgcc
//
LOCUS       DASSFQ010000176         7676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_674584, whole genome shotgun sequence.
ACCESSION   DASSFQ010000176 DASSFQ010000000
VERSION     DASSFQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674584"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2284)
                     /locus_tag="VFH96_06170"
     CDS             complement(<1..2284)
                     /locus_tag="VFH96_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET6973592.1"
                     /translation="MAAHIQEKPRVILPRAGRGNEILAILFIAFGLLLALCLISAAFY
                     PNDPSWNSVGGTETHNWAGAIGANVAATGFQLIGLAAFLLPLLLFAAAVRRFHTATIH
                     RPLMRIVGLLILVVAASALLSISHLHPLFDSSVQPGGLLGAVVAQGLASGLSTVGATV
                     LLVAIAAMGLLLATNFSFVRLYEILANAIGSRFAFVGKLPERFRAWRVMRAEQARLKK
                     ETKAAIKAEREAAKNALKQTVNLTPAERVADFMKEPIKPVVEPAAVIRTEAKSTTIEP
                     QATAVAASAGAGVGIAAPPVVTGRSGRRSIIPDVVDEEDDAEEMVKGASVLRVKADEP
                     ATAKLPFDASRRVNYSDYGLPTLDFLNEAPPHSEQADAELLSLATRLAEKCKEFNVTG
                     QIKHICPGPVVTTYEFKPDPGVKYSRVVSLVDDLCLALKAESIRIDRMPGKPHVGIEV
                     PNPRRETIFLREVIESRAFQESGSKLTIALGKTIDGLNYVADLAKMPHLLIAGTTGSG
                     KSVGVNALIVSILYRARPDEVKFILIDPKRLELGLYEDIPHLATPIIVDPKLASRSLK
                     WAVAEMERRYRDLAGWGVRNIDGYNTEIMRRNLIKEYDDKGEPWKPLPYIVIIIDELA
                     DLMMTSGREVEESITRLAQMARAVGIHLVLATQRPSVDVITGLIKANFPSRISFRVSS
                     KIDSRTIIDTNGAEQLLGRGDMLFLPPGTSRLIRVHGAYIDETEIGKIVAHIKAQGPP
                     SYDESITQSEEDALGLESASG"
     gene            complement(2314..3421)
                     /gene="prfB"
                     /locus_tag="VFH96_06175"
     CDS             complement(join(2314..3351,3353..3421))
                     /gene="prfB"
                     /locus_tag="VFH96_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006089.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET6973593.1"
                     /translation="MDPLSIQELRTNFDTLRARVDQLGGFFDVPAGREELNRIEAQAS
                     SPDFWSDQEAAQKLLQDRSRLEKKIERQEGFESQIADAEVLFEFAEEDESSIKELQPL
                     LESLEHELSQAETEMLLAGDNDRRNAICTIHPGAGGTESQDWAEMLLRMYLKWAEQRG
                     FKAEVLEYQPGEEAGLKSVTFRVEGEYAYGLLAAEAGVHRLVRISPFDQAARRHTSFA
                     SLFVYPEIDEDIEVEINDKDLRVDTYRATGAGGQHINTTDSAVRITHIPTGIVVSCQN
                     QRSQHQNRAVAMQVLRSRLYELELERRRAETAELEANKADISFGSQIRSYVLAPYQLV
                     KDTRTKLERGNVEAVLGGDIDDFIKAFLLARKAA"
     gene            complement(3427..4974)
                     /gene="lnt"
                     /locus_tag="VFH96_06180"
     CDS             complement(3427..4974)
                     /gene="lnt"
                     /locus_tag="VFH96_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET6973594.1"
                     /translation="MKRFDIRNYEWGAAAASALLLILSFPNFEYYQLAWIGLVPLLVV
                     IGRRPSPLRAFILGWAVGSVFFYATCYWLTYSMIHYGGLPTALAYLLLIPGALVVGIF
                     HGLFAALIALAIQKWGRMTVLLAPIFWITFEWVRLVVTGQLWNALGYSQAYHQWVIQP
                     ATWGGVYALSFLIVLINSVIALLIIRWTKLSYFVAVGLAVAVWLVIVFSGLVLIDRSD
                     LQDNHFVTIVAVQPNVPMNPVKSTEETTALVERHVALSTTGLATPSYAEGPRLVIWPE
                     SPMNFTYTSNKTFQEFVANFTRQNHTSLLFNSLEPAPNDGSYNSALLVNEEGRLISQY
                     DKIRLMPFGEYVPLPQWLPGASLISGIVGEFTPGSNYTLMPFGDRRAGVFICIESAYP
                     WIARRLAHDGADVLINISNDGYLGPTAVMRQHLANAIFRAVENGRPVLRVTNSGLSAE
                     ISAEGHVYGLTEPFQADVRVWVNQPGYERNTFYTRHGDLFVYVCAVISAIIFIAAVAD
                     RITRFTR"
     gene            complement(5485..7233)
                     /locus_tag="VFH96_06185"
     CDS             complement(5485..7233)
                     /locus_tag="VFH96_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973595.1"
                     /translation="MIFALLLTLVVTSAQAQNPTADVVMVLPFENTSKPFRAEYNWVG
                     ESFADALAELLNKPGLVVVSSDERELAYQQLRLPETVIPSRATAIKLARQAKASIIVI
                     GSYTVTPAPQNQAENSEKTSGGYVQVTARVIKVNEGRNFGEVKDGGWVTRQFDRGDAL
                     TALQEIHGWLAYQILYQRDKALPYSQNQLVQEATKIPQKAFEAYVKAVQLPLRSDVRA
                     NYLKNALRYYAESLGGAVYPQAAFELGRGYMDLGNWKDATEYFSKLQKKEPHYAEAAF
                     YASLGYTKLGDYGRALATVVPLSSDLPLIAVYNNAGAVAVQAARENKNGEERTRLLNQ
                     GMDFLGQASKSAPSDQMVNFNYGYGLFLAGKYKEATEHFRPVITADQQDGQAYFLFSK
                     SLAKLGNAEAAAAADDMARRYLRTAYAKWETEWQKSGSTSGVSLRMRDVLNREEVFGL
                     DREKNITAAMGSPSSATQDLLVKARDLYQAGRDEEALPELHRVVMIEPTNAEAYLLSG
                     RINLRRSDQEAAIAALKTAIFWDPKLIDAHILLGKIFLERGDRGEARKYALSAMAIDP
                     NNQEAIALQRQVTMGN"
     gene            complement(7294..>7676)
                     /locus_tag="VFH96_06190"
     CDS             complement(7294..>7676)
                     /locus_tag="VFH96_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HET6973596.1"
                     /translation="FSQSVSPYIDYVMHQDGSLVVNNQTVHLYKFWDATAFAEYLYEC
                     VAETIRTDLKEEIGFLRALDGAIRATMEIVDMPDRRASMLVRLILQNNGRLSHAKRST
                     FPELKDEEISAIEEAVARTQEDQR"
BASE COUNT         1688 a         2156 c         2115 g         1717 t
ORIGIN      
        1 caccggaagc gctctcgagt ccgagtgcgt cttcttcaga ttgtgtgatg ctctcgtcgt
       61 acgacggcgg gccttgtgcc ttgatgtgtg cgacgatctt gccaatctcg gtctcgtcga
      121 tatacgcgcc gtgaacacgg atcaatcgcg aagtacccgg cggcaggaac agcatgtcgc
      181 cgcgtccgag cagttgctcc gcgccgtttg tgtcgatgat tgtgcgcgag tcgatcttcg
      241 atgacacgcg gaacgaaata cgcgacggga agtttgcttt gatcagcccg gtgatcacat
      301 cgactgacgg tctctgagtc gcgagcacga gatgaatacc gactgcgcgc gccatctgcg
      361 cgaggcgcgt gatggactct tcaacttcac gccccgaagt catcatcaga tcggcgagct
      421 cgtcgatgat gatgacgatg tacggcaacg gcttccacgg ctcgcctttg tcgtcgtatt
      481 ccttgatgag gttgcgacgc atgatctcgg tgttgtagcc atcgatattc cgcacacccc
      541 agccggcgag gtcgcgatac cttcgctcca tctccgccac ggcccacttg agcgagcgcg
      601 acgcgagctt cggatcgacg atgatgggcg ttgcgagatg tggaatgtct tcgtagagac
      661 cgagctcgag gcgcttcgga tcgatcagga taaacttcac ttcatccgga cgcgcgcgat
      721 aaagaattga gacgatcagt gcgttcacgc cgactgactt accagagccc gtcgtaccgg
      781 caatcagcag gtgcggcatc ttcgcgagat ccgcgacgta gttcaaacca tcgatcgttt
      841 tgccgagcgc gatcgtgagt ttcgatcccg attcctgaaa cgcgcgcgac tcgatcactt
      901 cgcgcaggaa gatcgtttca cgacgcgggt tcggcacttc gataccgacg tgcggcttac
      961 ccggcatgcg atcgatgcga atcgactccg ctttcaacgc caaacacagg tcgtcgacaa
     1021 gcgacacgac gcgcgaatac ttcacacccg gatcgggctt gaattcgtaa gtcgtcacca
     1081 ctggaccagg acagatgtgt ttgatctgac cggtgacatt aaactctttg cacttttcag
     1141 ccagccgggt cgcaaggctc aaaagctccg cgtcggcctg ctcgctgtgt ggcggcgctt
     1201 cgttgagaaa atcgagcgtc ggcaaaccgt aatcgctgta gttaacgcga cgtgacgcgt
     1261 cgaacggcag tttcgcagtc gcaggctcgt cggctttgac gcgcaacacg gaagcgcctt
     1321 tgaccatctc ttccgcatcg tcttcttcat caacgacatc aggaatgatc gatcgccgtc
     1381 ctgatcttcc agtaacgact ggaggtgcgg cgataccaac accagcacca gcacttgccg
     1441 cgactgctgt tgcctgtggc tctatcgttg ttgatttcgc ctccgtgcga atcaccgccg
     1501 ccggctcaac aacaggcttg atcggctctt tcatgaaatc cgcaacacgc tctgcaggcg
     1561 tcaaattcac agtctgcttc aacgcgttct tcgccgcttc gcgttcagcc ttgattgccg
     1621 ccttcgtctc tttcttcagt cgtgcctgct cagcacgcat cacgcgccat gcgcgaaaac
     1681 gctctggaag tttcccgaca aaggcgaatc gcgaaccgat cgcattcgcg aggatctcgt
     1741 aaagtcgcac aaacgagaaa ttcgtcgcga gcaacaatcc catcgctgcg atcgcgacca
     1801 gcaacaccgt cgccccaacg gtactgagtc cactcgccag tccctgggcc acgactgcgc
     1861 cgagcagtcc acccggttgc acactcgaat caaacaacgg atgcagatgc gagatcgata
     1921 acaacgccga cgccgccacg acgaggatca agagtcccac gattcgcatc aacggcctgt
     1981 gaatcgtggc cgtgtggaag cgtcgcactg cggctgcgaa taaaagcaac ggcaacagga
     2041 aagcggcgag accgataagc tgaaagccgg tggcggcaac attcgcgccg atcgcgccgg
     2101 cccagttgtg cgtctccgtt ccgcccacgg agttccacga cggatcattc gggtagaacg
     2161 cggccgagat gaggcagagc gccagcagta atccaaacgc gatgaagaga atcgcgagga
     2221 tctcattgcc acgaccagcg cgcggaagaa tcacgcgcgg cttttcttga atgtgtgctg
     2281 ccatttaaaa ctctccaaca cgaactgtga tgcctacgct gccttgcgag ccagcaggaa
     2341 ggctttgatg aagtcgtcga tgtcgccgcc gagcactgcc tcgacgttac ctcgttccag
     2401 cttagtgcga gtgtctttta cgagctgata gggcgccagc acataacttc tgatttggga
     2461 cccgaatgaa atgtcggcct tgttggcttc gagctcggcg gtttcggcgc gccgtcgttc
     2521 caactcgagt tcataaagac gtgagcgcag cacctgcatg gcgacggctc gattttgatg
     2581 ttgtgaacgt tggttttgac aggatacgac gatcccggtc ggtatgtgag taatacgaac
     2641 tgctgaatcg gtagtgttga tgtgttgtcc gcccgcgccg gtggcgcgat aagtatcgac
     2701 acgcaagtct ttgtcgttga tctctacttc gatgtcctcg tcgatctccg gatagacaaa
     2761 cagagatgcg aatgaagtgt gtcgccgcgc cgcctggtca aatggcgaga tgcggacgag
     2821 acggtggacg ccggcctcgg cagccaaaag gccgtaggcg tattcacctt cgactctgaa
     2881 tgtaacgctc ttcagtcccg cttcctcgcc tggttgatac tcgagcactt cagctttgaa
     2941 gccgcgttgc tcggcccact tgagatacat gcgcagcagc atctcggccc agtcctgcga
     3001 ctcggtgccg cccgcgcccg gatgaatcgt gcaaatagcg ttgcggcgat cgttgtcgcc
     3061 ggcgagcagc atttccgttt cggcctggct caattcgtgt tcgagtgatt caagtaacgg
     3121 ttgcaactct ttgattgacg actcgtcttc ttccgcaaac tcaaagagca cttcggcgtc
     3181 ggcgatctgt gattcgaaac cttcctgacg ctcaatcttc ttttcaagtc gactacgatc
     3241 ttgcagaagt ttctgtgcgg cctcctgatc gctccagaag tcgggcgatg aggcctgggc
     3301 ttcgatacgg ttcaactctt cgcgacctgc agggacatca aagaaacctc ccgagctggt
     3361 ccacacgggc gcggagagtg tcgaagttag tgcgtagttc ttgaattgat aaagggtcca
     3421 tcttgttcat cttgtaaatc ttgtaatcct gtctgcaact gctgcaataa aaatgatcgc
     3481 tgaaatcacc gcgcaaacgt aaacgaacaa atcgccgtgg cgcgtgtaga aagtatttcg
     3541 ctcataaccc ggttgattca cccagacacg aacatccgcc tggaacggct cagtcagacc
     3601 gtagacatgg ccctctgcgc ttatctccgc cgacaagcca ctgttcgtaa cgcgcagcac
     3661 cggtcgaccg ttttccaccg cgcgaaagat cgcgtttgcg agatgttgtc gcatcacggc
     3721 tgtcggtccg agatacccgt cgtttgagat attgatcagc acgtccgctc catcatgagc
     3781 gagccgccgc gcgatccacg ggtacgctga ctcgatgcag atgaagacgc cagccctgcg
     3841 atcaccgaac ggcatcaacg tgtagttgct gcccggagtg aactcaccga cgattccact
     3901 tatcaaagac gctcccggta accattgcgg gagcggcaca tattcgccaa acggcattag
     3961 gcgaatctta tcatactgcg agatcaggcg gccctcttca tttacgagca gcgcggaatt
     4021 gtatgagcca tcgttcggcg ccggctcgag cgagttgaag aggagcgacg tgtggttctg
     4081 tctggtgaag ttcgcgacga actcctgaaa tgttttgttg gacgtgtacg tgaagttcat
     4141 cggcgactcg ggccagatca cgaggcgtgg tccttcggca tagctcgggg ttgcgagtcc
     4201 tgttgtgctc agcgctacgt gtcgctcgac gagcgcggtg gtttcttctg tactcttgac
     4261 cgggttcatc ggcacgtttg gctgaacagc gacgatggtt acgaaatggt tgtcctgcaa
     4321 gtcggagcgg tcaatcagca ccagtcctga aaacacgatc accagccaga cggcgactgc
     4381 gagcccgacc gccacaaagt agcttaactt tgtccatcgg atgattagta gagcgattac
     4441 tgaattgatc aaaactatga gaaaactaag cgcatagaca ccgccccacg ttgcgggctg
     4501 gatcacccac tggtgatagg cctgcgagta accgagtgcg ttccagagtt ggcccgtgac
     4561 cacgagtcga acccattcga aagtgatcca gaatatgggc gccagcagga ctgtcatgcg
     4621 tccccacttc tgaatcgcga gtgcgatcag tgctgcgaag aggccatgaa agatgccgac
     4681 gacgagagcg ccggggatga gtaatagata ggccagcgcg gtcgggagcc cgccgtagtg
     4741 aatcatcgaa tacgtcagcc agtaacaggt ggcgtagaag aagactgaac caacggccca
     4801 acccagtatg aacgcgcgca atggtgaggg gcgtcgcccg atcacgacga gcagcggaac
     4861 aaggccgatc caggcgagtt ggtaatactc gaagttagga aacgagagga taaggagtag
     4921 cgcgctggca gcagccgcac cccactcgta attcctaata tcgaaccgtt tcatccgctg
     4981 caaaaaattt ttttctaact gaccgaaaca tcgtaaatcg aatacgtcaa ttagccaaat
     5041 attgacgcaa aaagacagaa accgtcactg tcaaagtgac aaatttcggc aagccacaaa
     5101 aagcacaaaa ggcacaaaag aagtaatggc agcaacgtga ttctctcttt tgtgcctctt
     5161 gtgctttttg tggctgtatt taaaagcggg aagacccggt catttctgac caggtccacg
     5221 tttcgggggg aagcactgaa ggaacgctcg gaaggttagc tggcgcagcg gcaggcggcg
     5281 ctcagggaat gttggcggcg ggggacatcg gcttaacttc ctttccgtgt cgtgccgcgt
     5341 tccctttgag cacgcgccgt gccacaaagg agcacaaaaa tgattagcca caaaaaggca
     5401 cataagacac aaaagccttt tcaggaaaaa ctcttttgtg tttatgtgcc tttttgtggc
     5461 taaaaactct tctgccttta tggctcagtt acccatagtg acctggcgtt gcagcgcgat
     5521 tgcttcctgg ttgttcggat cgatcgccat tgcgctcagc gcatacttgc gcgcttcacc
     5581 gcggtcgcct cgttcgagaa agatcttgcc caagagaatg tgtgcgtcga tgagtttggg
     5641 atcccagaag atcgcggtct tcaaagccgc gattgctgct tcctgatcgc tgcgccgcag
     5701 attgatgcga ccgctgagta aatacgcttc ggcgtttgtc ggctcgatca tcactacgcg
     5761 atgtaactca ggcagcgctt cctcgtcgcg cccggcctgg taaagatcgc gagctttcac
     5821 gagcaggtcc tgcgtcgcgg agctgggcga gcccatcgcc gcggtgatgt tcttttcacg
     5881 gtccagacca aacacttcct cgcgattcag tacgtcgcgc atgcgcaggc taacaccgct
     5941 ggtcgatccc gatttctgcc attcagtttc ccacttcgca tatgctgtgc ggagatagcg
     6001 acgcgccata tcgtcggcgg ccgccgctgc ttctgcgttg ccgagttttg cgagcgattt
     6061 ggagaagagg aaataggctt gaccgtcctg ctggtcggcg gtgatcaccg gacgaaagtg
     6121 ctcggttgct tccttgtact tgcctgcgag aaataatcca tagccgtaat tgaagttgac
     6181 catctgatct gatggcgccg acttcgaagc ctgaccgaga aagtccattc cctggttcag
     6241 cagacgcgtg cgctcctcgc cgttcttgtt ctctcgtgcg gcctgaactg caactgcgcc
     6301 ggcgttgttg taaacagcga tcagcggtag atcactcgaa aggggcacta ctgtcgcgag
     6361 cgcgcggccg taatcaccaa gctttgtgta accgagactc gcgtagaaag cggcttcggc
     6421 gtagtgcggt tctttcttct ggagctttga aaagtactca gttgcgtctt tccagttgcc
     6481 gaggtccatg taaccgcggc ccagctcgaa agcagcctgt ggataaactg cgccaccgag
     6541 cgactccgcg tagtaacgca gagcattctt taaataattc gcgcgcacgt cgctgcgcag
     6601 cggcaactgc accgccttca cgtaagcctc aaacgccttc tgcggaatct tcgtggcttc
     6661 ctgtacgagc tggttttgcg agtagggaag cgctttgtcg cgttgataaa gaatctggta
     6721 cgcgagccag ccgtgaatct cctgcagtgc ggttagtgcg tcgccgcggt caaactgtcg
     6781 cgtaacccag ccgccgtctt tcacttcacc gaagttgcga ccttcgttga ccttgatcac
     6841 acgcgcggtc acctgcacgt aaccaccaga agttttctca ctgttttcag cttgattctg
     6901 aggcgcaggc gtgacggtat aactaccaat gacgatgatg cttgcttttg cctggcgcgc
     6961 gagcttgatg gctgtggctc gcgacggaat caccgtctcg ggcaagcgta gctgttggta
     7021 cgcgagctcg cgttcatcgc tggaaacaac gaccagaccg ggcttattca atagctcggc
     7081 aagggcgtcg gcaaaactct cgccgaccca gttgtactca gcgcggaacg gtttcgaagt
     7141 gttttcaaac ggcagcacca tcacgacatc ggcggtcgga ttctgggcct gcgcgctggt
     7201 tacaacgagt gttaataaaa gagcgaatat caatttaaga cctaaaaatc gcatgcctaa
     7261 ctttaccacg gttgccacgg atctaaagac cgatcaacgc tgatcttcct gggtacgagc
     7321 cacagcttct tcgatggcag agatctcctc atccttgagc tcgggaaacg tggagcgttt
     7381 tgcatgcgat agccggccat tgttttgtag gatcaagcgt accagcattg aagcgcgccg
     7441 atcgggcata tctacgattt ccatggttgc ccgaatagct ccatccaaag ctctcaggaa
     7501 tccgatctcc tccttgagat cggtgcgaat ggtttcagcc acgcattcat ataaatattc
     7561 cgcaaacgcg gttgcgtccc agaatttata aaggtgcaca gtctggttat tgacgacgag
     7621 cgatccgtcc tgatgcatca cgtagtcgat gtacggtgag acggattgag agaagc
//
LOCUS       DASSFQ010000177         5746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1411030, whole genome shotgun sequence.
ACCESSION   DASSFQ010000177 DASSFQ010000000
VERSION     DASSFQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411030"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..556)
                     /gene="atpH"
                     /locus_tag="VFH96_06195"
     CDS             complement(14..556)
                     /gene="atpH"
                     /locus_tag="VFH96_06195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET6973597.1"
                     /translation="MSLQTVARRYATALADVAIERREEREVQKELDQWAAMIESNPQL
                     KEVFVNPTIIHDHKRKVLEDLIARSRVRDTSASFLRVLLKNQRLSQLRDIAARYSQVL
                     DERAGMLAGSVTTARPISEDLKKTLHETLSAATGHTVRLTFETDEKIIGGLVARIGST
                     VFDGSVESHLERLADGMAGN"
     gene            complement(553..1062)
                     /locus_tag="VFH96_06200"
     CDS             complement(553..1062)
                     /locus_tag="VFH96_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET6973598.1"
                     /translation="MLLLAAEWYNYPGLELWKFANLAIFTGVGIYILRKPISQALQSR
                     RGAIEQELINAQNERDQALARVAEADSMLSGLDNDVRKVQEQAREEVTSERQRINVST
                     EREIEKLKQQAQREMETADKLSRKELRQFLAEKSVQMARESIRTQMRPEDDTALIRES
                     IGELRRTTV"
     gene            complement(1078..1524)
                     /locus_tag="VFH96_06205"
     CDS             complement(1078..1524)
                     /locus_tag="VFH96_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET6973599.1"
                     /translation="MILAEASIQLVPDGTLLLHLVLVVVMVFVLNRTLLKPINDILSE
                     REKQITGRLREAEAMAAESQEKLKKYNDTLREARAAGYRLLEKERAEGLKEKEQQLRQ
                     RRDELKKEVSSQVDQIRKQEQAVKGELEAQAATISSLISSQILRRH"
     gene            complement(1800..2663)
                     /locus_tag="VFH96_06210"
     CDS             complement(1800..2663)
                     /locus_tag="VFH96_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET6973600.1"
                     /translation="MNRKALGRGLGALLSSDSTIDLGSEPSEVEIDSIVPGSMQPRTH
                     FDEASLEGLADSIRTHGIVQPLLVRRRDGGYELIAGERRWRAAKLAGITRVPVVVREV
                     ADDNLLEIALIENIQRENLNPIEEAQAYKKLIETVGLTQESLATRVGRDRSYITNYLR
                     LLRLPDDVQQLVKEGRLSTGHARTLLALDQVDLQRRLARQIIDGGLSVRATEQLVQKA
                     TEEKPARRSAPQAVDPNIKAAETKLRRALGTQVKILQAADGRGRVEISFFNTQDLDRL
                     YNLLMPRGIEV"
     gene            complement(2614..3414)
                     /locus_tag="VFH96_06215"
     CDS             complement(2614..3414)
                     /locus_tag="VFH96_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET6973601.1"
                     /translation="MAKTIAVANQKGGVGKTTTAVNLAAIIASWGSRVLLVDADPQGN
                     ATSGIGVQRGSFRRGLYHTVVLDEPLSSIALSTSIPTLSVVPSNKDLAGAEVELVGLD
                     QREFRLKSALAKVEGDYDYIFIDCPPSLGLLTLNALTAAKSLLVPIQCEYYALEGVTE
                     LFDTLARLRRLHNPGLMIEGLLLTMYDERTNLSAAVAADLRDFYGLQVFQTVIPRNVR
                     LAEAPSYGKPIISYDANSKGADAYSQLAREIIHESKSARSGAGGSPVV"
     gene            complement(3491..5395)
                     /gene="mnmG"
                     /locus_tag="VFH96_06220"
     CDS             complement(3491..5395)
                     /gene="mnmG"
                     /locus_tag="VFH96_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET6973602.1"
                     /translation="MYWKVDMSFDEAFDVIVIGAGHAGCEAASAAARLGAETGLITLN
                     LDLVGQMSCNPAIGGIAKGHLVREIDALGGIMGRVIDRTGIQFRLLNRSRGPAVQSPR
                     AQADRMRYRAEMRRTLEATSNLHLRQGKVVDFVLDQGRLAGVVLEDGRRFGADAVVVA
                     TGTFLNGLIHTGRRTYSGGRAGEPASIELSETLKRLGFPVGRLKTGTPPRLDGRTVDW
                     SAFEPQPGDEMPVPFSFSTDNIEQKQIQCYIGYTTPDVHDAIRENIAESPLYSGKIKG
                     IGPRYCPSIEDKVVKFSDKSRHQLFLEPEGHDTNEVYLNGFSTSLPAEMQQELVRMIP
                     GLEETRIIRPGYAIEYDFVDPRELGPDLQTTRLPGLFHAGQINGTTGYEEAACQGLLA
                     GINAALYTQKRESLRLRREESYIGVLVEDLIKQGVDEPYRIFTSRAEYRLALRYDNAD
                     ERLERYGKELGLVGDSDWERFNSRQARLAKIRTALETVRLRRSDAQHAAVSALIGNDL
                     GDSISLEQLAKRPKVTAQLIMSLLPAELKPVAETDLNSVLADSLYAGYLDTQKAAIAK
                     LYQHDTLKIPRHTTFKSIAGLSNEIVERLDRAQPLTFGEARRLPGLTPGALSTLLAFL
                     SAQQKTGVNG"
     gene            complement(5380..>5746)
                     /locus_tag="VFH96_06225"
     CDS             complement(5380..>5746)
                     /locus_tag="VFH96_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973603.1"
                     /translation="NKSDLPGFNVPASCIEELRAINVSARTREGLATLRAAILASVSS
                     NGVDDGGLLITNARHYDLLCSTQLALEAARAALRERHSEELVLVPLHNALKFLGQITG
                     ETTTEDILSEIFATFCIGK"
BASE COUNT         1173 a         1705 c         1531 g         1337 t
ORIGIN      
        1 ttgaatccta tatttagttg cccgccatac cgtccgccaa tcgttcgaga tggctctcca
       61 ctgatccatc gaagacggtc gagccgatgc gcgcaaccag accaccaatg atcttttcat
      121 cagtctcaaa agttaaacga accgtgtggc cggtggcagc cgaaagcgtc tcgtgcaatg
      181 tcttctttaa atcttctgag atcggtctgg ccgtggtcac gcttccggcc agcatgcccg
      241 cacgctcgtc gagcacctgg ctgtatcgcg ctgcaatgtc tcgcaactgc gagaggcgtt
      301 gattcttcaa caagacgcgc agaaaactcg ccgacgtgtc tctcacgcgg ctgcgcgcga
      361 tcaagtcttc caaaactttt cgtttgtgat cgtgaatgat tgtaggattg acgaagactt
      421 cttttaactg cgggttcgac tcgatcatcg cggcccactg gtccaactct ttctggactt
      481 ccctctcttc gcgtcgttcg atagccacat cggcgagggc cgtagcgtag cggcgtgcaa
      541 ccgtttgcaa gctcaaaccg tggtcctcct caactcacca atgctttccc ggatcaacgc
      601 cgtgtcgtct tcaggccgca tctgagtgcg aatcgactcg cgcgccattt gcacgctttt
      661 ctccgcgagg aattggcgga gttctttacg cgacagctta tcggcggttt ccatttctcg
      721 ctgcgcttgc tgttttaact tttcaatctc gcgctcggtg gaaacgttga tgcgctgccg
      781 ttcggacgtt acttcttcgc gggcttgttc ctgaactttg cgaacgtcat tgtccagtcc
      841 gctcaacatg ctatcggctt cggcaacacg cgccaacgcc tggtcgcgct cgttctgcgc
      901 gttgattagt tcctgctcta tcgccccgcg tcgcgactga agcgcctggc tgattggttt
      961 gcgcaggatg taaattccca cgccggtgaa gatggcgagg tttgcaaact tccacagctc
     1021 aaggcccggg tagttgtacc attctgcggc tagtaacagc attgttttct atggacttta
     1081 gtgtcgacgc aatatctgag aacttatcaa actgctgatc gttgcggctt gcgcctcgag
     1141 ctcgcccttg actgcctgtt cctgctttcg aatttgatcg acttgcgagg agacttcctt
     1201 ttttaactcg tctcgccgct gtcggagttg ttgctctttt tctttgagac cctcggcacg
     1261 ttccttttca agcagtctgt agccggcggc gcgcgcttcg cgcaaagtgt cgttgtactt
     1321 cttgagtttc tcctggctct cggccgccat cgcctcggct tctctgagcc gtccggtgat
     1381 ctgtttttcg cgctcgctga ggatgtcgtt gattggcttc agcagggtcc gattcaggac
     1441 aaaaaccatt accaccacca acacgaggtg gagcagtaac gtaccgtcag gaaccaactg
     1501 aattgaggcc tctgcgagaa tcatccgatt cctctattgt gcgcaattta tggctgtcga
     1561 aaagcgcgcg agtctaacat ggcgcaaaaa cagaatcaac agaccgagcg ggcgcctaga
     1621 ggttcagttt gatgtccgat aagctttagc ttgtcggaat gtcgcgataa actaaagttt
     1681 atcggacact tgcagcgaaa gtgaccacca ccagcgggca tgacgactaa tggatttttc
     1741 tctgcagatt tcttatttta cattatgctg tctgaactag ggcgaattga tggtatcttt
     1801 taaacttcga taccgcgtgg catgaggagg ttgtagagac ggtccaagtc ctgagtgttg
     1861 aagaagctga tctcaacccg acctcgacca tcagccgctt ggagtatctt cacctgagtg
     1921 cccaacgccc ttctgagttt cgtttccgca gctttgatat tcgggtcgac ggcttgaggg
     1981 gcagatcgtc tggccggctt ctcttccgtc gccttttgaa ctagctgctc agttgctcgc
     2041 accgacagtc cgccatcaat gatctgccgc gcgagccggc gctgcaggtc gacctgatca
     2101 agcgccaaca gcgttcgagc gtgaccggtt gagagccggc cttctttaac taactgctgg
     2161 acatcgtccg gtagacggag cagacgaaga tagttcgtga tgtacgagcg gtcacgtccg
     2221 acgcgcgttg caagcgactc ctgtgttagt cccacggtct cgattaattt tttgtaagcc
     2281 tgcgcttctt cgatcggatt gaggttctcg cgttgaatat tctcgatcag cgcgatctcc
     2341 agcaggttgt cgtcggccac ttccctgacg accaccggca ctcgagtgat tcctgccagt
     2401 ttagccgcgc gccaacgccg ctcaccggca atcagctcat agccgccgtc gcggcgtctc
     2461 accaggagcg gctggacgat gccgtgcgtc cggatggaat cagcaagacc ctcaagactc
     2521 gcttcgtcga agtgagtccg cggctgcatc gatcccggca caatggagtc gatttcgact
     2581 tccgacggtt cagatccgag gtcaatcgtg ctgtcagacg acaggagagc ccccagcccc
     2641 cgacctagcg cttttcgatt catgaataat ctccctggct aattgtgagt atgcgtcagc
     2701 ccccttggag tttgcgtcat aactgattat cggcttcccg tagctgggcg cctcggccag
     2761 cctgacattt ctgggaatca cggtctgaaa aacctgcaaa ccgtagaaat cgcgcaagtc
     2821 tgccgcaacc gcggcagaca gattggtccg ctcatcgtac atcgtcagca gcaagccttc
     2881 gatcatcagg cccggattat gtagccgccg cagacgtgcg agcgtatcga agagctcagt
     2941 tacgccttcc agcgcgtagt attcacactg aatcgggacg aggagcgact ttgccgcagt
     3001 cagagcattg agcgtcagga gaccgagcga cggagggcaa tcgataaata tgtagtcgta
     3061 gtcgccctca accttagcga gagcgctctt cagtcgaaac tctcgctggt cgagcccgac
     3121 caactcgact tccgcgcccg cgagatcctt attagacgga acgacactca acgtcggaat
     3181 cgacgtcgac agcgcgatgc tcgaaagggg ctcgtcgaga acaacggtgt gatagaggcc
     3241 tcgccggaag ctgccgcgct gaacgccgat gcctgacgtg gcattgcctt gcgggtctgc
     3301 gtcgacaagc agaacacgac tgccccagga ggcgatgatc gctgcgagat tgacggcggt
     3361 agtggtcttt ccaactccgc ctttttgatt ggcgacggct atcgtcttag ccatggttgg
     3421 cgctaaacct aacacacgag gggctctgtg ggctacaccg ggaatgtttc acgtgaaaca
     3481 ttaactcaca ttaaccgttt acgccggtct tttgctgggc tgagaggaac gcgagcagcg
     3541 tggagagcgc gccgggcgtg agtccgggca gccgtcgcgc ttcgccaaac gtgagcggtt
     3601 gagcgcgatc gagccgttca acaatctcgt tcgacagccc cgcaatgctc ttgaatgttg
     3661 tgtgtcgcgg aatcttcaac gtgtcgtgct ggtatagttt tgcaatcgcc gccttctgcg
     3721 tgtccagata accggcatac agggaatccg ccagcacgga atttagatcc gtttctgcta
     3781 ccggcttgag ctccgccggc aacaacgaca tgatcaactg cgccgtcact ttcggacgct
     3841 tcgccaactg ctccagcgaa atcgaatcac caagatcatt cccgatgagc gccgacaccg
     3901 ctgcatgctg agcatccgag cgacgcaggc gcacagtttc cagcgcagtc ctgatcttcg
     3961 cgagacgagc ttgtcgtgag ttgaagcgct cccaatcgga atctcccacg aggccgagtt
     4021 ccttgccgta gcgctcaaga cgttcgtcgg cattgtcgta acgaagcgcc agccggtatt
     4081 ccgcacgcga ggtgaagatt cgataaggct catcgacacc ctgcttgatc agatcctcga
     4141 cgaggacacc gatatacgac tcttcacgcc gcagccgcaa tgactcccgc ttctgcgtgt
     4201 agagcgctgc gttaatccct gccagcagtc cctgacaggc agcttcctca taacccgttg
     4261 tcccattgat ctggcccgca tgaaagagac cgggcaatcg cgtggtttgc agatcaggcc
     4321 cgagctctcg cgggtcgacg aagtcgtact caatcgcgta gccgggccga atgattctcg
     4381 tttcctccag cccgggaatc atgcgcacga gctcctgctg catttccgcc ggcagtgagg
     4441 tcgaaaagcc gttcaaataa acttcgttcg tgtcgtgtcc ctcgggctcg agaaagagct
     4501 ggtgcctcga cttgtcagaa aacttcacga ccttgtcttc tatagaagga caatatcgcg
     4561 gcccaatacc cttgatcttt cccgaataaa gcggcgactc tgcgatgttc tcgcgaatcg
     4621 cgtcgtgcac atccggcgtc gtatagccga tgtagcactg gatctgtttc tgctcgatgt
     4681 tgtcggtcga gaacgagaac ggcaccggca tctcatcgcc tggctgcggc tcgaatgcgc
     4741 tccagtccac cgttcggcca tccagacgcg gcggggttcc ggtcttgaga cgaccaaccg
     4801 gaaatccgag ccgcttcagt gtctctgaca actcgatcga ggcgggctcg ccggcgcgac
     4861 ctcccgaata agtgcgacgt ccggtgtgaa ttagtccatt caaaaaagtg ccggtcgcga
     4921 caaccaccgc gtctgcacca aaacgacgtc catcttccaa cacgacgccc gctagtcttc
     4981 cttgatcgag cacgaaatcg acgactttcc cctgtcgcaa atgaagattc gaggtcgcct
     5041 cgagcgtgcg cctcatttcg gcgcgatagc gcatgcgatc ggcctgcgct cgcggcgact
     5101 gaactgcggg accacgcgat cgattgagca gccgaaactg aatgccggtg cgatcgatga
     5161 cgcgacccat gatgccgccg agcgcgtcta tctcgcgcac gagatgcccc ttggcaattc
     5221 cgccaatcgc gggattgcac gacatctgac cgaccagatc gaggttcagt gtaatcagac
     5281 cggtctcggc gccgaggcgt gccgcggcag acgccgcctc gcagcccgcg tggcccgcgc
     5341 cgatcacgat tacatcgaat gcttcatcaa acgacatgtc tactttccaa tacaaaacgt
     5401 agcaaatatc tccgacagta tatcttccgt cgtcgtctca ccggtgatct gtcccagaaa
     5461 ctttagtgca ttgtgcagcg gcacaagcac aagttcctca ctgtgtcgct cgcgtagcgc
     5521 cgcacgtgcc gcttctagcg ccaactgcgt gctgcacaac agatcgtaat ggcgcgcgtt
     5581 cgtaattaac aaaccaccat catcgacgcc gttgctgctc acacttgcga gaatcgcggc
     5641 gcgcagcgtc gccaaacctt cacgggttcg tgccgataca ttaatcgctc gcagctcttc
     5701 aatacaactt gcgggaacgt taaagccggg cagatcgctt ttgttc
//
LOCUS       DASSFQ010000178        19181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_978626, whole genome shotgun sequence.
ACCESSION   DASSFQ010000178 DASSFQ010000000
VERSION     DASSFQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19181
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978626"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VFH96_06230"
     CDS             complement(<1..1307)
                     /locus_tag="VFH96_06230"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6973604.1"
                     /translation="MIGQTVSHYRILEKLGEGGMGAVYLAEDLHLARRVAIKFLTATD
                     HHYRARFIREARAVSALSHINIAMVHDYGETTTGQPFIVMEYVKGKSLSDLLDEGLTL
                     NRTVEIISAVAEALGEAHQQGIVHRDIKPSNVLVNERGQVKVVDFGLVKHLFEPPSSD
                     VDLDAKTIYATKTRSDVIVGTPLYLSPEQATGKEVDGRSDLFALGALLYECMTGQSAF
                     SGGSVLEIGAQIIHVTPPPPSKINPNVTPALDRVTLKALEKKVDSRYQTAAEFLKDLK
                     DAATGLSGNSVLVSSRPAKPTEGFKRATNALGTLTMQLRRQRFSIASVIPVFIGAGLL
                     IWAAYYFWPRSYYKPSSAAALWYDQGTDNLRNGAFYQASKALAQAIVIDDKFALAHAR
                     LAQAWTELDYTDKAKDELLTVAKLRERSPLSQRDTLYLDAILAT"
     gene            complement(1449..1946)
                     /gene="purE"
                     /locus_tag="VFH96_06235"
     CDS             complement(1449..1946)
                     /gene="purE"
                     /locus_tag="VFH96_06235"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET6973605.1"
                     /translation="MAEKNGNKPLVAVIMGSQSDWDTMRHADETLTQFGVDHECRVIS
                     AHRSPSLAAEFGSTAESRGLEVVIAAAGGAAHLAGVLAAHTTLPVLGVPMKSDALNGL
                     DSLLATVQMPGGIPVATLAIGKPGAINAALFAVAILANSRPELRQKLHEFRAAQEKKI
                     RETVL"
     gene            complement(1948..3396)
                     /locus_tag="VFH96_06240"
     CDS             complement(1948..3396)
                     /locus_tag="VFH96_06240"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET6973606.1"
                     /translation="MSQRIPITVANGDGIGPEIMAATLRILEASGAPLEIETIDIGEK
                     VYLAGNSSGIEPSSWESLRRTKVFLKAPITTPQGGGYKSLNVTVRKVLGLYANVRPCV
                     SYHPFVETKHPVMDVVIVRENEEDLYAGIEHRQTNQVVQCLKLISRPGSEKIIRYAFE
                     YARRNNRKKVTCFTKDNIMKLTDGLFHKIFDEIAAEYTDLENEHWIVDIGAAKLADTP
                     EAFDVIVMPNLYGDVLSDVAAQIAGSVGLAGSANIGEHVSMFEAIHGSAPRRAGQNLA
                     NPSGLLLGAVMMLVHVGLTDHAERVHNAWLRAIEDGIHTYDIYDDKVSKQKVGTKEFA
                     DAVIKRLGQTPQILKAKKYSKGEESHASAGASRKAPQKELVGVDVFLDWTKGSPNDLG
                     DALGKVNGDGVKLTMISNRGVKVWPGGHKETFCSDHWRCRFLTEANSGKVAHAQVVSL
                     LGRIADAGYDFIKTEGLYTFDGERGFSLDQGE"
     gene            3773..4150
                     /locus_tag="VFH96_06245"
     CDS             3773..4150
                     /locus_tag="VFH96_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6973607.1"
                     /translation="MATKLYVGNLSFRTTSEELREAFASVGTVESASVIEDRETGRSR
                     GFAFVEMATPEEAAAAIEAFNGKDFGGRNLTVNEAKPRTDRGGGGRGGYGGGRGGGGG
                     RGGYGGGGGRDRDNDRYEREPRW"
     gene            complement(4212..5954)
                     /locus_tag="VFH96_06250"
     CDS             complement(4212..5954)
                     /locus_tag="VFH96_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6973608.1"
                     /translation="MKSKRFTSLVAALLLATFAAVAQQAETEPSAAKLQQHVTYLASD
                     ELDGRRTGTAGANGAAKYIAREFERLGIRPAPATDSRKINVMLARYLQTFPYIGTVEL
                     GKNNVLSVRGSETLNFRAGEDWMPLGISINKKLELTEVVFAGYGITANELNHNDYKGT
                     YSKTQVAVIQKGSPDGDNPHGKFTTAGQLRFKVAAAQSAGVGALLIISSEDDLKNDAL
                     AKLHYDNVGLTGIPVAVISKQTAEKLAKAKQVTLTTDVVRADVPAHNVVGVIEGSDPV
                     LKNESIVIGAHYDHLGRGGEGSLAPRSGEIHHGADDNASGTAGVIELARIFSTQRPKL
                     KRTLVFIAFGGEEEGLLGSNYYVNHPLMPLDKTVAMINMDMIGRMKDRKLVIGGVGTA
                     KEWRQLINTGSNGAFELTMNEDGYGPSDHSSFYSKQIPVLFFWTGTHNDYHKPSDTSE
                     KINYADEARILSLVARIVTDIDAADPRLTFTTAKSDPPRGGGFRVYLGTIPNYADSSD
                     GLLLDGIRDDSPAAKAGLKAGDKIVKIGNREVKNVYDYTAALGDMKAGQEYVIEVKRG
                     EEKLILKITPQSRN"
     gene            complement(5975..6988)
                     /locus_tag="VFH96_06255"
     CDS             complement(5975..6988)
                     /locus_tag="VFH96_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973609.1"
                     /translation="MILFSAAPATTQQNQLVLPNEKHLQNLKQLTFGGENAEAYFSAD
                     GKQLIFQSTREGHGCDQIYTMNIDGSNVKMISNGDGRTTCSYFFPDAKRVLYSSTYLG
                     DKQCPPRPDFSKGYVWAVYPTFDIFTAKPDGSDLKQLTNTPGYDAETTITLDGKKLVF
                     TSMRDGDLDIYTMDANGKNVRRLTNELGYDGGPFWSYDGKQIVYRANHPQTDQQKADY
                     LDLLKQNLIRPTTLEIWVMDADGSNKRQVTSNGKANFGPYFFPDGKRIIFASNMDDPR
                     GRNFDLYKINTDGTGLERITFNDTFDGFPMFSPDGKKLVFASNRAAKTPGETNVFIAD
                     WVE"
     gene            complement(7018..7848)
                     /gene="lgt"
                     /locus_tag="VFH96_06260"
     CDS             complement(7018..7848)
                     /gene="lgt"
                     /locus_tag="VFH96_06260"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET6973610.1"
                     /translation="MYPELFRIGNFPINTYGVFLALAFLCAILIAVRLARRDGLPAEK
                     IYDLRLWMLLAGLVGSKVLMLFTEPDYRDNPALLLSLDFLRSGGVFYGGLLGAVLAGY
                     FLMKRYKLPWWKTADACAPGIAVANFFGRQGCFAAGCCWGKPTGLPWGVKFTELGHQI
                     TGVPTDVHLHPTQLYESFAMLLVFFFLLWLHKRKRFDGQVILAYALLYSAIRFAIEFV
                     RDDPRGDVFGLTSLTGLSTSQLISLVIGIWALILLIRRRRRANSVNLENPVILSKTAV
                     "
     gene            complement(7838..8377)
                     /gene="lspA"
                     /locus_tag="VFH96_06265"
     CDS             complement(7838..8377)
                     /gene="lspA"
                     /locus_tag="VFH96_06265"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET6973611.1"
                     /translation="MDTSIEKRADFRTRFAWRAGYLLAAFGLYMADQASKAWAVRALR
                     FGDERVVIRGFLQFIYTENPGIAFGQLQEGGSFGRWFFVVLAVLAAIAVFYYFMRTPR
                     NDDRVLGACALLLAGISGNLTDRARLGYVIDFIVLHAGDYHWPTFNIADASITVGALL
                     LAYDLIFSHRSTRIKADVS"
     gene            complement(8429..11383)
                     /gene="ileS"
                     /locus_tag="VFH96_06270"
     CDS             complement(8429..11383)
                     /gene="ileS"
                     /locus_tag="VFH96_06270"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET6973612.1"
                     /translation="MSTETPLDLKKTVNLPKTAFAQKANLAQAEPARLEKWNKLDLYR
                     LIRRAGEGRPKFIMHDGPPYANADIHLGTAMNKILKDFIIKSRTMMGFDTPYVPGYDC
                     HGLPIETFVEKDLEKKGKKKNELSVEVFRRICREHAEKALKSQTRDFKRLGILGEWDD
                     PYLTMSNHYEAETARMFARFVERGWVYKGARPVYWCLKDQTALAEAEVEYHQHTSPSV
                     YVKFPLQSDPALIDPALAGRKVFLIIWTTTPWTLPANLGITVHPDFEYSAFEYGDEVY
                     IVASELVGAVAGKCGLGSDLKVLARFPGSKLDRLEARHAWLDRPSLLMVGEHVTLGGE
                     SDAETELDVSEAQKKADHKAGTGLVHTAPGHGHDDFVIGKRYGLEIYCPVDNAGRFTP
                     EVEHFAGQRVFDANDPIVEFMREQGVLLFSEKYDHRYPHCWRCKNPVIFRATPQWFIA
                     MDHAHTDLVEKDEDGRDRSNFTDNLSETPAVSLRAAALSEIERVNWIPGWGVDRMRNM
                     QKGRPDWCVSRQRVWGVNIPVFYCAHCTEAVADPSVINHVADIFERESGDAWYTHPAE
                     ELLPPGYTCKGCGAQEWTKETDILDVWFDSGTSSVAVLENRPELRWPADVYIEGGDQF
                     RGWFNSSLLIGIAAHDRAPYKTVITHGWTLDAQGRAMHKSAGNAVEPEKVIKQSGAET
                     IRLWCASTNYFDDMRASDEILQRVTDGYRKLRNTARFALGNLDGFEPARDTVPYDELE
                     EIDRWALAELGHVTKEVLAAYEAYEFHNVYRALFNWATVALSARYFDIIKDRLYTFAP
                     RNKARRSAQTVLLAIADALARMLGPILVFTADEIWENLPDRAEPSIHLALLPKANPND
                     DKELLANWEKLFWVRELVLATLEKARELKRIGSSLEAEITIETGKSNFELLKAHEANL
                     RYLFIVSKVTLLEKPELAGTQVNVEVTEAPGEKCERCWNYSTHVGESERYPTVCERCI
                     AALSEIEEGATD"
     gene            complement(11527..12102)
                     /locus_tag="VFH96_06275"
     CDS             complement(11527..12102)
                     /locus_tag="VFH96_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973613.1"
                     /translation="MNLALLLQMSTNDPMFWIMVIIAVSFVFIAVAMIALAVFVSRAV
                     KTVNRLEEKVEPLLERVTAISEQGKLVAVQGKLIAEQFTVVSGHLSTATGHVSESLAV
                     IKSEIIELKELVSDTAVEARDKVELVSRTIDRTHLQVAQTTDYVQSKVIEPAREIAAI
                     MAGFRRGLEVLVAPMPKPINQTYAEDEMFIG"
     gene            complement(12108..12518)
                     /locus_tag="VFH96_06280"
     CDS             complement(12108..12518)
                     /locus_tag="VFH96_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET6973614.1"
                     /translation="MGDESISNKLTYLLIGGGIGAILALLFAPKSGHELRGDIADATR
                     KGIDRSREAAQQLGDRAGEYYEATRGRAAELYSQAAERVSDAAQSARDAASRQTGTLT
                     AAIDAGKKAYQEEKRKTEMSGRIEAPNFGGESTN"
     gene            complement(12624..14693)
                     /locus_tag="VFH96_06285"
     CDS             complement(12624..14693)
                     /locus_tag="VFH96_06285"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6973615.1"
                     /translation="MHEAREGIINRLAMTLPHAINLDRFLNVIVSELGRMMNVDRCDV
                     IQLKSASELRISHEWRASNDVPSSLGTVIPLDLKQLQEHVDLRKPIRLDDISASGLDQ
                     KVRYLAASLGTRSLLVVPVMLGEDVLGLVGLHTTHSPREWLDDEVSFLQSIAGQIAIG
                     YQYTRLYTDKEREAETKRALLSIANALNARSDFGEVSSLVLERAIALVHADYAALGVL
                     DPAGSRLSLAAFKAAPDAAVDSVHGLFDSHGKSIDLTTFPAMIEVLAQGKTLPLLETD
                     LPFTWRMAFNVVLGARAALVAPVRVGGQTFGLLGLVWSQVRDGFEDHDVALVEGIADQ
                     IGTALERDQLSAEVLHLRSALHERYGEERIIGQTPVIRRAIELALSVADTQTTVLIEG
                     ESGTGKELLANLIHFNSGREDQPYVKLNCGAIPETLLESELFGHEKGAFTDARQLRRG
                     RFEEASGGTLFLDEVGEMSPSAQVKLLRVLQDGEFMRVGGSEVIKTDVRVLAASNVDL
                     KRAVDLGSFRQDLFYRLSVFPIRLPPLRERPEDIHPLVIHFLEHYKQKTGRFISGISK
                     DALQALITYDWPGNVRELENAIERAVIIASGRAIELEDLPEAISKIALQDRDRIKVER
                     AKAASEGRTTTFEVTVPSSMEEIERLAIEATLDYTDGDKSHAARALGIGRKTLYRKLD
                     QYSNGQS"
     gene            14849..15163
                     /locus_tag="VFH96_06290"
     CDS             14849..15163
                     /locus_tag="VFH96_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6973616.1"
                     /translation="MPIYEYECKKCKAHVEAFQKLSDKPLTKCRKCGGKLEKKISAPA
                     IQFKGSGWYVTDYAGKTTKSEKSESESTSESKTEKTDKKKESSPQKTSDKTSPPKAST
                     GE"
     gene            15181..16071
                     /locus_tag="VFH96_06295"
     CDS             15181..16071
                     /locus_tag="VFH96_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973617.1"
                     /translation="MRSRSKLLITLLVIGLVSISILAQSGVRPNANAAGGVMIGVVAR
                     RDNKQTNPVTSNDLAIFDNGIQQSIRNFTPDPSPARIVLLVDNSLTIRADVEKLEQAA
                     REFAYEIYEGDKLLIVGYDEQAEIVSDWTDDAKAIESSLKLFRKKGQPHLFDAIRAVV
                     EEAMRPMQNPKRIIVVISDGLDRGSKATFEQTLAELQTENIMVYAVQAVDRTRGAIRR
                     DVPKPKVVIDKLAEGTGGQIFSIDEPSVAAKAICDELRQNRYVLSYVPSSAPFGQARS
                     LLVVGNEGITVRSKSMQQPN"
     gene            complement(16138..16299)
                     /locus_tag="VFH96_06300"
     CDS             complement(16138..16299)
                     /locus_tag="VFH96_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973618.1"
                     /translation="MIWIAGGVLMGTYVAYSNAFTDDGEFSFSFFVFMEALILLIMGG
                     MFYFYSRKG"
     gene            complement(16320..17222)
                     /locus_tag="VFH96_06305"
     CDS             complement(16320..17222)
                     /locus_tag="VFH96_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973619.1"
                     /translation="MKLFILIFFVVVCFTPAVAQQQADPEFDTSVAKPAYKKDGPRVM
                     FDEAHHNFHTSDGRYKPFVDLLVNDGYNVIRNRQPFVKTRLSGFKILVIANALGAEED
                     DDKGADDSAFTTEEIAAVADWVKGGGALLLIADHAPFGGAASALANSFGVDMSKGYTF
                     DPANSVAGSPTQLIFSRENKLLANHPIADGRNETERINLVRSFTGQSLKGPEGSVEIL
                     KLADSATDKPDFAAETSTPAAGRAQAIALKFGKGRVVVQGEAAMLSAQISGADKHTMG
                     MNVPGNDDKQYALNLLHWLSGILK"
     gene            complement(17287..18486)
                     /locus_tag="VFH96_06310"
     CDS             complement(17287..18486)
                     /locus_tag="VFH96_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6973620.1"
                     /translation="MSTASPSKSRYVSLKASSFTESVIREMTREALKYGAVNLSQGFP
                     DFSAPADIKRRAMEAIDQDINQYAITWGSKDFRDAIAAKTHSYLGIEIDPEAEITVTC
                     GSTEGMIAAMMATVDPGEEVIVFEPFYENYAPDAILSDAHPIYVPLRAPDWTFDRDQL
                     RAAFNKKTKAIIVCNPNNPTGKVFSRDEMEFIAGLCQEFDALCFTDEIYEHILYPREG
                     AEIKHISMAQLDGMRERTVVVNSMSKTYSVTGWRVGYCIAPPEITGAIRKVHDFLTVG
                     AAAPLQAAGAYALSLPQEYYDTMQREYQRRRDMLLPVLEQSGFGTFAPDGAYYIMTDI
                     SKFGFADDVEFTRHLIREIGVACVPGSSFYSNPADGAQQVRFCFCKKDETLHSAAERL
                     SKLKSKI"
     gene            complement(18570..>19181)
                     /locus_tag="VFH96_06315"
     CDS             complement(18570..>19181)
                     /locus_tag="VFH96_06315"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973621.1"
                     /translation="SDPAELKFQEAEAAREKARDDPSLQNAADLYREAIKLRPAFPAA
                     QVGLARVLMSLNEFKDAHAAIDAARRARPAYAEASAVEGRIYREEAFDDDAIRSFRRS
                     IREGGGVQPEAYVGMAKVWEDKGQFAEAIVEYKKALAQLSDSEPVIYQMLGAAYERVE
                     KPKEAVTAYEKYLELAPNGSYAAAIRSILTQLKREAAGEQIIP"
BASE COUNT         4194 a         5516 c         5198 g         4273 t
ORIGIN      
        1 gttgccagga tcgcgtccag atacaaagtg tctcgctgcg aaagcggtga acgttccctg
       61 agctttgcta cagtcagcaa ctcgtccttt gctttatctg tgtaatcgag ctcggtccac
      121 gcctgtgcga gacgcgcatg ggccagggca aatttatcgt cgatcacaat ggcttgagcg
      181 agtgctttgc tggcctggta aaaagcgcca ttacgcagat tgtcggttcc ctggtcgtac
      241 cagagtgcag cggctgaaga tggtttgtag taacttcgcg gccagaagta ataggcggcc
      301 cagatcagta gtccggcacc gatgaaaacg ggaatcacgg atgcgattga aaaacgctgt
      361 cgtcgcagtt gcatcgtcag cgttcctaag gcattcgtgg cgcgcttaaa gccttccgta
      421 ggtttggctg gccggctcga gaccagcaca ctattgccac tcaagcccgt cgcggcatct
      481 ttcaagtcct tgagaaactc agcggccgtc tggtagcgag agtcgacctt cttttccaac
      541 gctttcaacg tcactcgatc cagcgcgggc gtcacgttgg gattgatttt cgacggcggt
      601 ggcggcgtga cgtgaatgat ctgcgcaccg atctccaaca cgctgccgcc tgaaaacgcc
      661 gactgcccgg tcatgcactc gtaaagcagc gctccgagcg cgaacaagtc actacgtcca
      721 tcaacctctt tgccggtcgc ctgctcgggc gaaagataga gcggcgttcc cacgatcacg
      781 tcactacgcg tcttcgtggc gtaaatcgtc ttcgcgtcga gatcgacatc gctcgagggc
      841 ggctcaaaca ggtgcttcac gaggccgaag tccaccactt tcacttgccc acgctcgttg
      901 acgaggacgt tcgaaggctt aatgtcgcgg tggacgatgc cctgctggtg agcttctccg
      961 agtgcttcag caacagccga aatgatttcc acggtgcgat tgagcgtgag cccttcatca
     1021 aggagatcgc tgagcgactt ccccttcaca tactccatca cgatgaacgg ctgaccagtc
     1081 gtggtctcgc cgtaatcatg gaccatcgcg atgtttatat ggctcagcgc cgagacagcc
     1141 cgtgcttcac gaataaagcg cgcgcggtag tgatggtcag tggcagtgag aaacttgatc
     1201 gcgacacgcc gcgcgagatg caggtcttcg gcgagataaa ccgcgcccat gccaccttcg
     1261 ccaagttttt ctagaattcg ataatgggag actgtctggc cgatcatact cgcctgggtc
     1321 catcgggttg cgagagagag acgtggtttg ggcgcgagtg tttacatggg gggattgtgc
     1381 gacaggatta caggattttc aagaaaatcc tgtaaatcat gcaaacccta taatcctgcc
     1441 aaaggccttt acagtacggt ctcccgtatc ttcttctctt gcgccgccct gaactcgtgc
     1501 agcttctgcc gcaactctgg tcggctgtta gctagtatcg cgaccgcgaa aagcgcggcg
     1561 tttatcgcac caggcttgcc aatcgcaagc gttgccaccg gaattccacc gggcatctga
     1621 accgtcgcca acagcgaatc aagcccgttt agcgcatcac tcttcatcgg cacgccgagc
     1681 accggtaacg tcgtatgcgc agcaagaacg ccggcgagat gggccgcgcc gcccgcggct
     1741 gctatgacaa cttccaaccc gcgggattcc gcggttgacc caaactccgc tgccagtgac
     1801 ggcgatcgat gagctgaaat cacccggcac tcgtgatcca ctccaaactg agtcagcgtc
     1861 tcgtccgcat gtcgcatcgt gtcccagtca gactggctac ccataatgac tgccaccagc
     1921 ggtttatttc catttttttc ggccatctta ttctccctgg tccaacgaga acccacgctc
     1981 gccgtcaaat gtataaagac cttccgtctt gatgaaatcg taacccgcgt cagcaatacg
     2041 accgagcagc gagacaacct gcgcatgcgc gactttgccg ctgtttgcct cagtcagaaa
     2101 ccgacagcgc cagtggtccg aacaaaaagt ttccttatgg ccgccgggcc agactttcac
     2161 gccccgatta ctgatcatcg tgagtttgac gccgtcgccg ttgactttac cgagcgcgtc
     2221 gccgagatcg ttgggcgagc ccttggtcca atcgaggaaa acgtcaacac cgacgagctc
     2281 tttctgcggc gccttgcgac tggcgccggc gctcgcatgc gactcctcgc ccttggaata
     2341 cttcttcgcc ttcaaaatct gtggcgtttg tcccaaccgc ttgatgacgg cgtccgcaaa
     2401 ctccttcgtg cccaccttct gcttgctgac tttgtcgtcg taaatgtcgt acgtgtgaat
     2461 accgtcttca atcgcgcgca gccacgcgtt gtgaacacgt tcggcgtgat cagttaagcc
     2521 gacgtggacc agcatcatca ccgcgcccag caacagtccc gacggattcg cgaggttctg
     2581 accagcgcgt cgcggcgccg agccgtgaat cgcttcaaac atcgaaacgt gctcgccgat
     2641 atttgcagat cccgccagtc cgaccgatcc ggcaatctgc gcggcaacgt cagaaagtac
     2701 gtcgccataa aggtttggca tcacgatcac gtcgaatgct tccggcgtgt cggcgagttt
     2761 tgccgcgccg atatcgacaa tccaatgctc gttttcgagg tcggtgtact cagccgcaat
     2821 ttcgtcaaag atcttatgaa agagaccgtc agtcagcttc atgatgttgt ctttcgtgaa
     2881 gcaggtgact ttcttgcgat tgttccggcg cgcgtattca aacgcgtaac gaataatctt
     2941 ttccgatcca ggacgcgaga tcagtttcaa acactgcacc acctggttcg tctgccggtg
     3001 ctcgattccc gcgtaaagat cctcttcgtt ctcacgcaca atcacgacgt ccatcaccgg
     3061 atgcttagtc tcgacaaacg ggtgatacga gacgcaaggc cgtacgttgg catacagccc
     3121 gagcacttta cgaacggtaa cattcaggct tttgtagcca ccgccctgtg gcgtggtgat
     3181 gggtgcttta agaaagactt tggtgcgtcg taacgattcc cacgagctcg gctcgatgcc
     3241 cgatgagttg ccggcgagat aaactttctc gccgatatcg atggtttcaa tctcgagggg
     3301 cgctccggaa gcttcgagga tgcggagcgt ggcggccatg atttccggtc cgataccgtc
     3361 accattggcc acagtgatag gaatacgttg cgacattttc caaacctttc gtgggtgatt
     3421 tctgaacgag ggacagatta tacggattac gaggaatttg cctaatatgt taaatctgtg
     3481 gctgaaaagt caaaattgtc cttgaccctt tccaagggct cctgcatact tttgcctacc
     3541 agttacctgc tctcgcgcat caacggtgat agcgcctgcc cgtgatcgtt aaggcgacga
     3601 ggttcggctg aatagagagt gaggcagttg tcaaccagtt agaagccgct ctcgagattt
     3661 ttttagagca ccgggttcgt cgcagctcgc cgaaagtatc ggtcgtcccc cccgaactgg
     3721 atcagcgaat tccagcaaga cccgcgcttc ccttcagata aggatagtta gaatggctac
     3781 aaaactctat gtcggaaatc tctcgtttcg tactacgagc gaggaactgc gcgaagcttt
     3841 cgcgtcagta ggtacagtcg aatcagcttc agtcattgaa gatcgcgaga caggacgatc
     3901 gcgcggcttt gcttttgtgg aaatggcaac ccctgaagaa gcagcagcag ccatcgaggc
     3961 tttcaatggc aaagactttg gcggacgcaa tctaaccgtc aacgaagcca agcccagaac
     4021 tgaccggggc ggtggtggtc gtggtggtta tggtggtgga cgtggtggtg gcggcggtcg
     4081 tggtggttac ggcggcggtg gcggccgtga tcgcgataac gatcgttacg agcgcgagcc
     4141 ccgctggtaa gattttgggc cacggattac acggatttaa tccggattta tctgtgtaat
     4201 ccgtggctaa attagtttct ggactgaggc gtgatcttta gaattaattt ctcttcaccc
     4261 cgttttactt caatcacata ctcctgaccg gctttcatat cgcccagcgc tgctgtgtaa
     4321 tcgtaaacat tcttcacttc acgattgccg atcttgacga tcttatcccc tgctttaagt
     4381 ccggccttgg ccgccggtga atcgtcgcgg atgccgtcga gcagtaatcc atcgctacta
     4441 tctgcgtagt ttggaatggt tccgagataa acacggaagc cgccaccgcg cggcgggtca
     4501 ctcttcgcgg tcgtgaacgt gagtcgcgga tcggctgcgt caatgtccgt cacgatgcgc
     4561 gccacgagac tcagaatgcg tgcttcgtca gcgtagttga tcttctcgga cgtgtcagac
     4621 ggtttgtgat agtcgttgtg cgttccggtc cagaagaaca gaacggggat ctgtttgctg
     4681 taaaacgatg aatgatcgct cggaccgtag ccgtcttcgt tcatcgtcaa ctcgaaagcg
     4741 ccgttcgaac cggtgttaat aagctggcgc cactctttcg ccgttcccac accgccgatc
     4801 acgagtttgc gatctttcat gcggccgatc atgtccatgt tgatcatcgc cacggtcttg
     4861 tcgagcggca tgagcgggtg attgacgtag taattcgaac cgagcagtcc ttcctcttca
     4921 ccaccgaaag cgatgaagac gagtgtgcgt ttgagctttg gacgttgtgt gctgaagata
     4981 cgcgcgagct cgatcacacc tgcggttccg gaggcgttgt cgtcagcgcc gtggtggatc
     5041 tcgcctgagc gcggcgcgag gcttccttca ccgccgcgac cgagatgatc gtagtgtgcg
     5101 ccgatgacga tgctctcgtt cttcagcact ggatctgagc cctcgatcac gccgacgacg
     5161 ttgtgtgccg gaacgtcggc acgtacgacg tcggttgtca gcgtgacttg ttttgccttt
     5221 gccagcttct cggctgtctg tttggaaatc acggcaaccg gaatgcctgt cagtccgacg
     5281 ttgtcgtaat gcagtttcgc gagtgcatcg ttctttagat cgtcttcact cgagataatt
     5341 aaaagcgcgc cgacacccgc gctctgtgcg gccgcgactt taaaacgtag ttgaccggcg
     5401 gtggtaaatt ttccgtgtgg gttgtcgccg tccggcgagc ccttttgaat cacggcaacc
     5461 tgggttttcg agtatgtacc tttatagtcg ttgtgattga gctcgttggc cgtgatgcca
     5521 tagccggcaa acaccacttc tgttaactcg agcttcttgt taattgaaat gccgagcggc
     5581 atccagtctt cgccggcgcg aaaattcaga gtttcgctgc cgcgaactga gagcacattg
     5641 ttcttgccca gctccacggt accgatgtat gggaatgtct gcaggtaccg cgccaacatt
     5701 acgttgattt tgcgagagtc agtggcgggc gcaggccgta tgccgagacg ctcgaattcc
     5761 ctggcgatat acttcgcggc cccgttcgcg cccgccgtgc cggtgcgtcg accatccaac
     5821 tcgtcagagg cgagatacgt gacgtgctgc tgaagcttcg cagccgaagg ctcagtctca
     5881 gcttgctgcg cgacagcggc aaacgtcgca agtaacagcg cagcgacgag agaagtaaat
     5941 cttttactct tcatttttgt gcctttttgc ggccctattc gacccaatca gcaataaaca
     6001 cgttcgtctc accgggagtt tttgcggcgc ggtttgaagc gaacacgagt ttctttccat
     6061 caggcgagaa catcgggaat ccatcgaacg tgtcgttgaa cgtgatgcgt tctaatccgg
     6121 tcccatccgt gttgatcttg tagagatcga aattcctccc ccgcggatcg tccatgtttg
     6181 acgcgaagat gattcgcttt ccgtccggga agaagtatgg accaaagttg gctttgccgt
     6241 tgctcgtcac ctgtcgcttg ttcgagccgt cagcatccat cacccagatt tcgagcgtcg
     6301 ttggacgaat caaattctgt ttcagcagat ccaggtagtc ggctttctgc tggtccgtct
     6361 gcggatggtt tgcgcgataa acgatctgct tgccgtcgta agaccagaat ggcccaccgt
     6421 catatccgag ctcatttgtg agccggcgca cgttcttgcc attcgcgtcc atcgtgtaga
     6481 tatcgagatc gccgtcacgc atcgacgtaa agacgagctt cttgccgtcg agtgtgatcg
     6541 ttgtttcggc gtcgtagccc ggagtgtttg tgagctgctt cagatctgag ccgtccggct
     6601 tggcagtgaa gatgtcgaac gtcggataca ccgcccaaac gtaacctttc gagaagtcgg
     6661 gtcgtggcgg acactgctta tctccgagat atgttgacga gtaaagaacg cgcttcgcgt
     6721 ccgggaagaa gtacgaacac gtcgtgcgtc catcaccatt cgagatcatc ttcacgttcg
     6781 agccgtcgat gttcatcgtg tagatctgat cgcagccgtg gccctcgcgc gttgattgaa
     6841 agatgagctg cttaccgtca gcggaaaagt aagcttcggc gttctcgccg ccgaaggtta
     6901 gctgcttgag attttgcaga tgtttctcgt tgggaagaac cagttgattc tgttgtgttg
     6961 tagcgggggc tgcactgaac agaatcacga ctaaaaacag gggtaatttt agtgagctca
     7021 tacggctgtt tttgacagga ttacaggatt ttcaagattt acagaatttg cgcgacggcg
     7081 tcgacggatc aagaggatca gcgcccagat cccgatgaca aggctgatca actgcgaggt
     7141 cgagagtccg gtcagtgacg tcagaccgaa gacgtcgcca cgcggatcgt cgcgcacgaa
     7201 ttcaatcgcg aagcgaatcg cggagtagag caatgcgtac gcgagaatca cctggccgtc
     7261 gaagcgtttg cgcttgtgca gccacagtaa aaagaaaaac acgagcaaca ttgcgaacga
     7321 ctcgtagagc tgtgtcggat gaaggtgaac gtcagtcggc acgcccgtga tctgatggcc
     7381 caattcggta aacttcacgc cccacggaag ccccgtcggc ttgccccagc aacagcccgc
     7441 ggcgaagcaa ccctggcgtc cgaagaaatt cgccaccgca atgccgggcg cacacgcgtc
     7501 agcagttttc caccacggca acttgtaacg cttcatcagg aagtagccgg cgagcacggc
     7561 gccgagcagt ccgccataaa acacgccgcc tgaacgcaga aaatcgagag acagcagcag
     7621 cgccggattg tcgcggtaat caggttcggt gaagagcatc aaaaccttcg agccgaccag
     7681 tcccgccagc agcatccaca acctgaggtc gtagattttt tcggccggca acccatcgcg
     7741 ccgcgcgagt ctaaccgcaa tcagaatcgc gcagagaaac gcgagggcga gaaacacacc
     7801 gtaggtgttg atggggaaat taccgattcg aaatagctca ggatacatcc gcttttatcc
     7861 gtgtcgatct gtggctaaaa attagatcgt acgccaacaa taacgctcca accgtaatgc
     7921 tcgcgtcggc gatattgaac gtgggccaat gatagtcgcc ggcgtgcagg acaatgaaat
     7981 cgatcacgta accgagacga gcgcggtcag tcaagttacc cgaaatccca gcgagcagca
     8041 gcgcacacgc gccgagcacg cgatcgtcgt tacgcggcgt gcgcatgaag taataaaaca
     8101 ctgcgatcgc tgcaagcacc gccagcacga cgaagaacca gcgtccaaac gagccgcctt
     8161 cctgtaattg cccaaacgcg atgccggggt tttcagtgta gatgaactgc aagaaaccgc
     8221 gaatcaccac gcgctcatcg ccgaagcgca gcgcccgcac cgcccacgct ttggacgcct
     8281 ggtcggccat gtacaagccg aacgcagcca gcaggtaacc agcgcgccag gcgaagcgcg
     8341 tgcgaaaatc ggccctcttt tcgatactgg tatccaaggt tatttggacg cggattaacg
     8401 cggaaataaa tccgattttt tatctgtgtt aatccgtggc tccttcttca atctcactta
     8461 gcgcggcgat gcagcgctcg cacaccgtcg gatatcgttc cgattcgccg acgtgcgttg
     8521 aataattcca gcagcgctcg cacttttccc cgggagcttc tgtaacctcc acattcactt
     8581 gtgttcctgc taactcaggt ttttccagta aggttacttt agatacgata aataagtagc
     8641 gcaaattagc ttcatgcgcc ttcaacaact caaaattcga cttgcccgtt tcgatggtga
     8701 tttcagcttc tagagaactt ccgattcgtt tcagctcgcg ggccttttca agcgtcgcaa
     8761 gaaccaattc acgaacccag aacaactttt cccaatttgc caacagctcc ttatcatcgt
     8821 tcggattggc cttcggtaac agcgcgagat ggatcgacgg ctccgcgcgg tcaggcaagt
     8881 tttcccagat ctcgtcagcc gtgaaaacga ggatcggccc gagcattcgc gcgagcgcgt
     8941 ctgcaatcgc gagcagcacg gtctgcgccg aacgccgcgc tttgttacgc ggcgcaaacg
     9001 tgtaaagccg atccttgatg atgtcgaaat agcgcgctga caacgccacc gtcgcccagt
     9061 tgaacaacgc gcgatagacg ttgtgaaact cgtaagcttc gtacgccgcg agcacttctt
     9121 tcgtcacgtg cccgagctcc gcgagcgccc agcggtcgat ctcttccaac tcgtcgtaag
     9181 gcacagtgtc gcgtgccggt tcgaatccgt cgagatttcc gagggcaaac cgcgcggtgt
     9241 tgcgcagctt gcgatacccg tcagtcacac gctgaaggat ctcgtcagac gcgcgcatgt
     9301 cgtcgaaata gtttgtcgac gcacaccaca aacgaatagt ctcggcgccc gactgcttga
     9361 tgaccttctc aggctcgacc gcgttgcccg cagacttgtg catcgcgcga ccctgcgcgt
     9421 cgagcgtcca gccgtgtgtg atcacggtct tgtacggagc gcgatcgtgg gccgcaatgc
     9481 cgatcaggag cgacgaattg aaccaaccac gaaactgatc gccgccttcg atgtaaacat
     9541 ccgcgggcca gcgcagctcc ggacgattct ccagcaccgc tacggatgat gtgccactat
     9601 cgaaccagac gtcgagaatg tcagtttcct tggtccattc ctgcgcgcca catcccttgc
     9661 acgtataacc cggcggtaac aactcttcgg ccggatgcgt gtaccaggca tcgcccgact
     9721 cgcgttcgaa tatatccgcg acgtgattga tcactgacgg atcagccact gcttcggtgc
     9781 agtgcgcgca gtagaaaaca ggaatgttta caccccaaac acgctggcgc gacacgcacc
     9841 agtcaggccg ccctttctgc atgttgcgca tgcgatcgac gccccaaccg ggaatccagt
     9901 tgacgcgctc gatctccgaa agcgcggctg cacgcaatga cacagcaggt gtttccgaca
     9961 agttgtcagt gaagttgctg cgatcgcgac cgtcttcgtc tttctcgacg agatcggtgt
    10021 gcgcgtggtc catcgcgatg aaccactgcg gcgtcgcccg gaagatcaca ggattcttac
    10081 agcgccagca atgaggataa cgatggtcgt acttctcact gaaaagcagc acaccctgct
    10141 cgcgcataaa ctcaacaatc ggatcgttcg catcaaacac gcgctgtccc gcgaagtgtt
    10201 cgacttcagg cgtgaaccgt cccgcattgt cgaccgggca ataaatctcg agaccgtagc
    10261 gtttgccgat cacaaagtcg tcatgaccat gaccgggcgc ggtgtgtacg agaccagtcc
    10321 cggctttgtg gtccgccttc ttctgcgctt cgctgacgtc aagctccgtc tccgcgtcag
    10381 actcaccgcc gagcgtcaca tgctcgccga ccatcaacag cgacggacga tcgagccacg
    10441 catgacgagc ttcaagccgg tcgagcttcg aacccggaaa ccgcgccagc accttcagat
    10501 cagatcccag gccacacttt ccagccactg ctccgaccag ctcactcgca acgatataaa
    10561 cctcgtcgcc atactcaaac gccgagtatt caaaatcggg atgcacagtg atgccgaggt
    10621 tggcaggcag cgtccacggt gtcgtggtcc aaatgatcag gaacactttg cggccggcga
    10681 gcgcgggatc gatcaacgcc ggatcgctct ggagcggaaa cttcacatac accgacgggc
    10741 tcgtgtgctg gtgatactca acctcagctt ccgcgagcgc cgtctggtcc ttgaggcacc
    10801 agtaaaccgg ccgtgcgccc ttgtagaccc agccacgctc gacgaaccgt gcgaacatgc
    10861 gcgcagtctc ggcctcgtag tgattcgaca tcgtgagata cggatcgtcc cactcgccaa
    10921 gtattccgag tcgtttgaaa tcgcgcgtct ggctcttcaa agccttctca gcgtgctcgc
    10981 gacagatgcg gcgaaacact tcgacactca actcgttctt cttcttgccc tttttctcga
    11041 ggtctttttc gacgaaggtc tcgatcggca gaccatgaca gtcgtaaccg ggaacgtagg
    11101 gcgtatcgaa gcccatcatc gtgcgcgatt tgatgatgaa gtctttcaaa atcttattca
    11161 tcgccgtgcc gagatggatg tcggcgttcg cgtatggcgg accgtcgtgc atgatgaact
    11221 tcgggcggcc ttcgccggcc cgacgaatga gtcgatagag atcgagcttg ttccatttct
    11281 cgaggcgcgc cggttcggcc tgcgccagat tggccttctg cgcgaaagcg gtctttggca
    11341 gattgacggt tttcttgaga tcgagcgggg tttcagtaga cataagcgta agtcgtaaat
    11401 agtaacaaag ccatccgcag attacacaga tggcagaaac aatctgcgta atctgtggtt
    11461 aaaaaactaa gaacccgagc catccaggat gagctcgggt ccgttcgaat caacttctaa
    11521 ttggaattaa ccgataaaca tctcatcctc ggcgtaggtt tgattaatgg gcttcggcat
    11581 cggcgccacg agaacctcga ggccacggcg gaagcccgcc ataatcgccg caatctcgcg
    11641 cgcaggctct atcaccttcg actgcacgta gtccgtcgtt tgcgccacct gcaggtgcgt
    11701 gcgatcgatc gtgcggctaa ccagctcgac tttatcccga gcctcgaccg ccgtatcact
    11761 caccagttcc ttcaactcaa tgatctcact cttgatcacc gccagcgact cggaaacgtg
    11821 acccgtcgcc gtcgacagat gaccggaaac taccgtgaac tgctcggcaa tcagtttccc
    11881 ctgcacggcg accagtttgc cctgctcgct aatcgcggtg acacgttcga gcagcggctc
    11941 aaccttctct tcgagccggt tcacagtctt cacggcgcgg ctgacgaaaa ccgcgagcgc
    12001 gatcatcgcc accgcaataa agacaaacga aacggcgatg atcaccatga tccagaacat
    12061 cggatcgtta gtgctcattt gtagcagcaa tgcgagattc attctggtta gttcgtcgac
    12121 tcaccaccaa agtttggcgc ttcgatgcgg cccgacattt cagtcttgcg cttttcttcc
    12181 tggtaagcct tcttgccggc gtcgattgcc gcggtcagcg ttccggtttg acgtgatgcc
    12241 gcatcgcgtg cactttgggc cgcgtcggaa actctctccg ccgcttgcga atacaactcg
    12301 gcggcacggc cgcgcgttgc ttcgtagtat tcaccggcac ggtcacccaa ctgttgcgct
    12361 gcctcgcgcg agcgatcgat acccttgcgg gtggcgtcgg cgatgtcgcc gcgcaattcg
    12421 tggcccgatt tcggggcaaa aagcagtgcg agaatcgcac cgatgccgcc gccgatgagc
    12481 aggtaagtca gtttgttcga gatactttcg tctcccattt tctggggtcc tttcttacaa
    12541 caaaattacg aggattacga ttttggacta tagcagccac aggaggtttt gccaagaata
    12601 gggcctataa gtcctatatt ttcttaagac tggccgttgc tgtactggtc cagcttgcgg
    12661 tagagcgtct tgcggccgat gccgagagcg cgggcggcgt gggatttgtc gccgtcggtg
    12721 tagtcgagcg tggcctcgat cgccaggcgc tcgatctctt ccatcgatga aggaaccgtg
    12781 acctcgaaag tcgtcgtgcg tccctcgctc gcggcttttg cacgttcgac tttgatccgg
    12841 tcgcgatcct gcaacgcgat cttgctgatc gcttcgggga ggtcttcgag ctcaatcgca
    12901 cgtccggacg caatgatcac cgcccgctcg atcgcgtttt caagctcacg aacgttgccg
    12961 ggccagtcgt aagtgatcaa cgcctggagc gcgtctttcg agattccgga aatgaagcgc
    13021 ccggtcttct gtttgtagtg ttcgaggaag tggatcacga gcggatgaat gtcttcgggc
    13081 cgctcgcgca aaggcggcaa cctaatcgga aatacggaga gcctgtagaa cagatcctgc
    13141 cgaaaacttc cgaggtcaac tgcgcgtttc agatcgacat tcgacgccgc gagcacgcgc
    13201 acatcagttt tgatcacttc gctaccgcca acacgcatga actcgccgtc ctgcagcacg
    13261 cgcagcaatt tcacctgcgc cgacggactc atctcaccga cttcatcgag aaacaacgtg
    13321 ccgccgctcg cttcttcaaa ccggccgcga cgcaactggc gcgcatcagt aaacgcgccc
    13381 ttctcgtgac caaaaagctc cgactcgagc agcgtctcgg gaatcgcgcc gcagtttaat
    13441 ttgacgtaag gctggtcttc gcgaccggag ttgaaatgga tcaaattcgc gagcagctct
    13501 ttacccgttc ctgattcgcc ttcgatcaaa actgtggtct gcgtatcggc gacgctcaac
    13561 gcgagctcga tcgcgcgccg tatcacaggc gtctgaccga tgatacgttc ctcgccgtag
    13621 cgctcgtgta gcgcgctgcg caggtgcaag acttctgccg acaactgatc gcgctcgagt
    13681 gccgttccga tctgatcggc gatgccttcg acgagcgcca catcgtgatc ctcaaaacca
    13741 tcgcgcacct ggctccaaac cagtccgagt aagccaaacg tctgtccacc aactcgcacc
    13801 ggagcgacga gcgccgcccg tgcaccgagc acgacgttga aagccatgcg ccacgtaaac
    13861 ggtagatcag tttccagcag gggcagtgtt tttccctgcg cgagcacctc gatcatcgct
    13921 ggaaacgtag tcaagtcgat cgatttgccg tgcgagtcga agagtccgtg aacggaatcg
    13981 accgccgcat cgggcgcagc cttgaacgct gcaagactga gacgactacc cgccggatcg
    14041 agcacgccaa gcgccgcata atcagcgtgg acgagggcga tcgcgcgttc gagcacgagc
    14101 gacgaaacct cgccaaaatc cgagcgcgcg ttcaacgcat tcgcgatcga caacagcgcg
    14161 cgcttcgttt cggcttcgcg ttctttatcg gtgtagagtc gcgtgtattg ataaccaatc
    14221 gcgatctgcc cggcgatcga ttgcaggaaa ctgacttcat catcgagcca ctcgcgcggc
    14281 gagtgcgtgg tgtgtagacc aacgaggccc agcacgtctt cgccaagcat gactggcacg
    14341 acgagtaacg atcgcgtacc gagactcgca gcaagatatc taaccttctg atcgagacca
    14401 gaagcggaaa tgtcatccag ccggatcggc ttccgcagat cgacgtgttc ctgaagttgt
    14461 ttgaggtcga gtggaataac agtgccgaga ctcgaaggca catcgttcga cgcgcgccac
    14521 tcgtgactga tgcgcagctc gctcgcagac ttcaactgga tcacatcgca gcgatcgacg
    14581 ttcatcatcc ggcccagctc ggaaacgatg acgttgagga agcggtcgag attgatggca
    14641 tgaggcaaag tcatcgcgag gcggttgata atcccctcgc gcgcctcatg cattttgatc
    14701 ttgcgttcca gagaaagcga ttgggcggtg ttggacatcg tacgggtaca gaggatatca
    14761 agttatcgtg taaatcctga aatcctgtga aaagacttac acaggattcc gggatgtaca
    14821 ggattcacat gattgctata atccctccat gccgatttac gagtacgaat gtaagaaatg
    14881 caaggctcat gtcgaggcct tccagaagct cagcgacaag ccgctgacaa agtgccgcaa
    14941 gtgcggcggg aagctggaaa agaagatctc cgcgcccgcg attcagttca agggctccgg
    15001 ctggtacgtc accgattacg ccggaaagac aactaaaagc gaaaagtcag aatccgagtc
    15061 aacgtccgag tccaaaaccg aaaaaacaga caagaagaaa gagagttccc cccagaagac
    15121 ttccgacaag acttcacctc cgaaagcttc aaccggcgaa tagccgctcc ggatttcaac
    15181 atgcgttcgc gatctaaact cctgatcacc ctcctcgtca tcggattggt ttcaatctcg
    15241 atcctggccc aatccggcgt tcgaccaaac gcgaacgcgg cgggcggtgt gatgatcggc
    15301 gtagtggcgc gacgggacaa caagcagacc aacccggtca cgagcaacga ccttgcgatt
    15361 ttcgacaacg gcatccaaca gtcgattcgt aacttcacac ccgatccgtc gccggcgcgc
    15421 atcgtgctcc tcgtcgacaa ttcactaacg attcgcgcgg acgtcgagaa gctcgagcag
    15481 gccgcgcgcg agtttgctta cgagatttac gaaggcgaca agctgctgat cgtcggttac
    15541 gacgaacagg ccgagatcgt ctcagactgg acagacgatg cgaaagcgat tgagtcgtcg
    15601 ttgaagctgt ttcgaaagaa aggccagcca cacttgttcg acgcgatccg tgcggtggtc
    15661 gaagaagcga tgcggccgat gcagaatccg aagcgcatca tcgtcgtgat cagtgacggt
    15721 ctcgatcgtg gcagcaaagc gacgttcgaa cagacgctgg cggagctcca gacagagaac
    15781 atcatggtct atgcggtgca ggcggtcgac cgcacgcgcg gcgcgattcg acgcgacgtg
    15841 ccgaaaccga aagtcgtgat cgataaactg gcagaaggaa cgggcgggca gatcttttca
    15901 attgatgagc cgtcagtagc agcgaaagcg atttgtgacg agctacgaca gaatcgttat
    15961 gttctctcgt acgtgccgtc gagtgcgccg ttcgggcaag cgcgtagttt gttagtggtc
    16021 ggcaacgaag gcatcaccgt ccgctctaag tcaatgcagc aacccaacta ggtttgatgt
    16081 ccgataagct ttagcttgtc ggtaggtcac gacaagctaa agcttatcgg acatcaacta
    16141 acccttcctc gaatagaaat aaaacatccc acccataatc agcaaaatca gcgcttccat
    16201 gaacacgaag aagctgaacg aaaattcgcc atcatccgtg aacgcgttcg agtaagcgac
    16261 gtacgttccc atcagcacac cgcccgcaat ccaaatcaat tgacgcttct tgtcccacac
    16321 tacttcaata tccctgacag ccagtggagc agattgagcg cgtattgctt gtcgtcgttt
    16381 ccgggcacat tcattcccat cgtgtgtttg tccgcccctg aaatctgcgc cgacaacatc
    16441 gccgcttcgc cctggaccac tacgcgacct ttcccaaact tcaatgcgat cgcctgagcg
    16501 cgtccagcgg caggcgttga cgtttcggct gcgaaatccg gcttatcagt tgccgagtca
    16561 gccagcttca gaatctcgac actgccttca ggtcctttca acgattgacc ggtgaaactg
    16621 cgcacgagat tgatccgctc agtctcattg cggccgtcag cgatcggatg atttgcgaga
    16681 agtttgtttt cgcgcgagaa gatcagttgt gtaggactgc cggcgaccga gtttgccgga
    16741 tcgaacgtgt aacccttgct catatcgacg ccgaagctgt tcgcgagtgc cgacgcggcg
    16801 ccaccaaacg gcgcgtgatc ggcaatcagc aacaacgcgc caccgccctt cacccaatcg
    16861 gcgacggcag cgatctcttc cgtagtgaac gctgaatcgt cggcgccttt gtcgtcatct
    16921 tcttcggccc cgagcgcgtt ggcgatgacg agaatcttga agccgctgag ccgtgtttta
    16981 acgaacggct gtcgattgcg aatgacgttg taaccgtcgt tcaccagcag atcgacaaat
    17041 ggtttgtagc ggccgtcgct ggtgtggaag ttgtgatgcg cttcgtcgaa catcacgcgc
    17101 ggtccgtctt ttttatacgc gggtttcgcg acggatgtat caaactccgg atcggcttgt
    17161 tgctgcgcta ctgccggcgt aaaacacaca acgacgaaga agataagaat gaagagcttc
    17221 atagtgatgc aaaaaaaggc atccacagat cacaccgatt actttctgtg aaatctgtgg
    17281 atggtcttaa atctttgact ttaatttcga cagtcgctca gccgcgctgt gcagcgtctc
    17341 gtctttttta cagaaacaga agcgcacctg ctgcgcgcca tcggcgggat tgctgtagaa
    17401 gctcgatcca ggcacacacg ccacgccaat ctcccggatt aaatgccgcg taaactctac
    17461 gtcgtccgca aagccaaact tcgagatgtc agtcatgatg tagtaagcgc cgtcgggtgc
    17521 aaacgttccg aatcccgatt gctcgagcac gggcaacagc atgtctcgac gcctctgata
    17581 ctcgcgctgc atcgtgtcgt aatactcctg cggcaacgac aacgcgtaag cgccagcggc
    17641 ttgcaacggc gccgccgcgc cgacggtcag aaaatcatgg accttgcgaa tggcacccgt
    17701 gatctcgggg ggcgcgatgc aataacccac gcgccagccg gtcacagagt aagttttcga
    17761 catcgagttc acgacgactg ttcgttcgcg catgccgtcg agctgggcca tcgagatgtg
    17821 tttgatctcg gcgccctcgc gcgggtaaag gatgtgctcg taaatctcgt cggtaaagca
    17881 cagcgcgtca aattcctggc aaagtccggc gataaactcc atctcatcgc gactgaaaac
    17941 ttttccggtc ggattgtttg gattgcagac gatgatggct ttcgtcttct tgttgaacgc
    18001 cgctcgcaac tgatcgcgat cgaaggtcca gtcaggcgcg cgcagcggca cgtagatcgg
    18061 gtgcgcatcg gaaaggatcg cgtcaggcgc gtagttctcg tagaacggct cgaagacgat
    18121 gacttcttcg ccgggatcga cggtcgccat cattgctgcg atcatgcctt cagtcgagcc
    18181 gcatgtgacg gtgatctccg cttccggatc gatctcgatg ccgaggtacg aatgcgtctt
    18241 cgctgcgatt gcgtcgcgaa aatctttcga gccccacgtg atggcgtact ggttgatgtc
    18301 ctgatcgatc gcttccatcg cgcgacgttt gatatcggcg ggcgcggaaa aatccgggaa
    18361 gccctgcgac aggttcacgg cgccatactt caacgcctca cgcgtcattt cccgaatcac
    18421 cgactcggta aacgagcttg ctttcagcga cacgtagcga ctctttgagg gtgatgctgt
    18481 actcatttca gaacatttag ccacaaaagg tacaaaaagc acaaaagata aagaggaaac
    18541 cacttttgtg ctttttgtgc cttttgtggc taaggaatga tctgctcgcc ggccgcttcg
    18601 cgtttgagtt gtgtgagaat tgagcgaatt gccgcagcgt agctgccgtt cggcgccagc
    18661 tcgagatact tctcgtaagc agtcacggct tctttcggct tctcaactcg ttcgtacgcg
    18721 gcgccgagca tctggtagat cacgggctcg ctgtcagaaa gttgggccag ggcttttttg
    18781 tattcgacga tcgcttctgc aaactggccc ttgtcttccc aaactttcgc catgccgaca
    18841 taagcttcag gttgtacgcc gccaccttct ctgatcgacc ggcgaaacga tcgtatcgcg
    18901 tcgtcgtcaa acgcctcttc gcgataaata cgaccttcaa ccgctgaagc ttcggcgtac
    18961 gccggcctgg ctcgccgcgc ggcatcgatc gcggcgtgtg catctttgaa ctcgttgagc
    19021 gacattagca cacgcgcgag accgacttgc gccgcgggaa atgccggcct gagtttgatc
    19081 gcttcacgat aaagatcggc agcgttttgt aacgacggat cgtcgcgcgc tttctcacga
    19141 gctgcttcgg cttcctggaa tttcagctct gccggatcgc t
//
LOCUS       DASSFQ010000179         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1856850, whole genome shotgun sequence.
ACCESSION   DASSFQ010000179 DASSFQ010000000
VERSION     DASSFQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856850"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFH96_06320"
     CDS             complement(<1..872)
                     /locus_tag="VFH96_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET6973622.1"
                     /translation="MSSTPPNVTIPPPAAPPLNFIAILREMLRASDKVSDLIFSPGRP
                     PQIELAGKLVPVRIAGLEKLTPAHTAGISKLIIGTQQTAVDSLEKNGSADLSFSVPGD
                     ARFRVNIFKQRGTHAIVMRVIPMRPPKFSDFNLPGQLADIVNIKNGIVLVTGPTGSGK
                     SSTLAAIIDLINETHHYHIVTIEDPIEFLHTHKNSTIHQRELHSDTPSFAVALRAALR
                     QAPKVILVGEMRDRETIEVALEAAETGHLVLSTLHTIDASKTVDRIIGVFPKNEEKVI
                     RTRIAQSFRFIISQR"
     gene            1041..1298
                     /locus_tag="VFH96_06325"
     CDS             1041..1298
                     /locus_tag="VFH96_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973623.1"
                     /translation="MATYRDPVTCFAPLEVIERLLEIRVEAGYSPAVYLLSPDDLADY
                     LDAYGFDVYALSEAEAEFHFGDYRAPLAPGGDHAHCSLTAH"
     gene            complement(1357..>2096)
                     /locus_tag="VFH96_06330"
     CDS             complement(1357..>2096)
                     /locus_tag="VFH96_06330"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET6973624.1"
                     /translation="EKKRLEGQLEVARQVQLELLPAHDPEITGYDISAYNFPTEEVSG
                     DYYDWVKIFDDQIAIVIADVAGKGVPAALLMAFLRASLRAATHIGYATHISMSKVNYL
                     LWESIERNQYVTAFYGILDASKGTLAYSNAGHNPPLLLNAAGTSRFIDKGEQPLGMFR
                     ETRYHEYYLDFEPGDVLVLYTDGATEASSPSGEEFGRERLAQAVKDNYDKPAREMIAS
                     LQMAVLEWTDNAGSNDDVTFFIIKALK"
BASE COUNT          433 a          575 c          605 g          483 t
ORIGIN      
        1 cgttgggaaa tgatgaagcg aaacgactgc gcgatgcgcg tgcgaatcac tttctcttcg
       61 tttttcggga acacgccgat gatgcggtcc accgtcttcg aagcgtcgat cgtgtgcagc
      121 gtggagagca ccaggtggcc cgtttccgcc gcctcgaggg caacttcaat cgtctcacgg
      181 tcgcgcatct caccgacgag aatcactttc ggcgcctggc gcagcgcggc gcgcagcgcg
      241 acggcgaagc tcggcgtgtc ggagtggagc tcgcgctggt gaatagtgga atttttgtgc
      301 gtgtggagaa actcaatcgg atcctcgatg gtcacgatgt ggtagtgatg cgtctcgttg
      361 atcaggtcga taatggctgc gagggtcgag ctcttaccgc tgccggtagg tccagtcacc
      421 agcacgatgc cgttcttgat attgacgata tccgccagtt ggccgggaag attgaagtcg
      481 ctgaacttcg gcgggcgcat gggaataacg cgcatcacga tcgcgtgagt gccgcgctgt
      541 ttaaagatat ttacgcgaaa acgcgcgtcg ccggggacgc tgaacgacag gtccgccgag
      601 ccgttcttct ctaaactatc gaccgccgtt tgttgtgtgc cgatgatcaa ttttgaaatt
      661 cctgcagtgt gcgcgggcgt caacttctcc aagccggcga ttcgaaccgg gaccagtttt
      721 cctgccagct cgatctgcgg cggccgtccg ggactaaaaa tcaggtcgct gactttgtcg
      781 gacgcgcgca gcatctcccg caaaatggcg atgaaattca acggcggcgc tgccggcggc
      841 ggaatggtta cgtttggtgg tgtgctactc atgattcagg aacttttcac tatgctcgcg
      901 cgtcaaaaga atgcgtgacg aaagattgcg tggcgaacaa actactttga gcgtacgtac
      961 atcaaagtcg tcgatataac aggcacggtg cacggaaatt tgaaccgtgg acctgatttt
     1021 ggaagaaagg cgctggacac atggcaacgt acagagatcc tgttacatgc tttgcaccac
     1081 ttgaagtaat cgagcgcctg ctggaaattc gtgttgaagc gggttactcg cctgccgtat
     1141 atcttctttc ccccgatgat ttggcagact atctcgatgc gtatggcttc gatgtgtatg
     1201 ctttatcgga ggccgaagct gagtttcact ttggcgatta tcgtgctcca ctagcaccgg
     1261 gcggagatca cgcccactgc tcgctgactg cacactagaa tcacaaatcg ttcaagaatt
     1321 agcaacgcgg gagactggaa acagctcccg cgtttgttat ttgagcgctt tgatgatgaa
     1381 gaaagtaacg tcgtcgttgg agccggcatt gtcggtccac tccaaaaccg ccatctgcaa
     1441 actcgcgatc atctcgcggg ccggcttgtc gtagttgtct ttcactgcct gcgcgagccg
     1501 ctcgcgccca aactcttcgc ccgatgggct cgatgcttcc gtggcgccgt ccgtgtaaag
     1561 caccagcacg tcgccgggct cgaaatcgag gtaatactcg tgatatctcg tttcccgaaa
     1621 cattcccagg ggctgctcgc ctttgtcgat aaatcgcgaa gtaccggcgg cattcaagag
     1681 caacggcgga ttgtgtccgg cgtttgaata agcgagagtt cccttcgacg cgtcgagaat
     1741 gccgtagaac gccgtcacat actggttgcg ttcgatcgac tcccacagca gatagttcac
     1801 tttcgacatc gagatgtgcg tcgcgtaacc gatgtgcgtt gccgctcgca gactcgcgcg
     1861 tagaaacgcc atcaacagcg ccgcaggcac gcctttgccc gccacgtcgg cgatgacgat
     1921 ggcgatctgg tcgtcaaaga tctttaccca gtcgtagtaa tcgccggaaa cctcttcggt
     1981 gggaaagttg taggcgctga tgtcgtagcc ggtgatttcc ggatcgtgcg ccggcaacag
     2041 ctccaactgc acctggcgcg cgacttcgag ctggccctcg agccgcttct tctcca
//
LOCUS       DASSFQ010000180         4900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1275098, whole genome shotgun sequence.
ACCESSION   DASSFQ010000180 DASSFQ010000000
VERSION     DASSFQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275098"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFH96_06335"
     CDS             complement(<1..979)
                     /locus_tag="VFH96_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6973625.1"
                     /translation="MEPSTQKLIHTFGALADLGQEIAGTGDFTEMVRTSMHLLLGTLA
                     LRRGAVVECSGPGPLRCVAIWGLTDRALHGFEIDEEQMESFLGGEIRESLVRNTSSAN
                     VFLGKYRNKLAGEGIELVVPMVVRGEVTGLVLLGGKASGEEFSNEDFETIRAMVRHIG
                     VGIHTHRLLEQLAQRAEENRRLYDELRTIYRDTVRAFAAAIDIKDKYTQGHSERVGRY
                     SEIIARELGWSEEEVEGIQIAGYLHDIGKLIVDRDIINAPYKIDAKNSIELNRHPNAG
                     YEILAPIRHPYADIPFMAKHHHERLDGRGYPDGLTDQQIPMGAKIVSLAD"
     gene            complement(982..1365)
                     /locus_tag="VFH96_06340"
     CDS             complement(982..1365)
                     /locus_tag="VFH96_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6973626.1"
                     /translation="MDTSSGGLVRSHCVGTTAVVYASDYLNKLSGEKIERECRRQLNC
                     GCRALVIDFSDTKLVNSIGISILLGIIDVAEKSGAQIVFSDVNNQTVQLFEMLGLTRH
                     VALAKDEREALLNLSHFETTTPVVH"
     gene            complement(1455..3713)
                     /locus_tag="VFH96_06345"
     CDS             complement(1455..3713)
                     /locus_tag="VFH96_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973627.1"
                     /translation="MSSRSENFRRVLGRVVQQDFVGRAAELDRIMAQLERANAGRGLL
                     LLMEPSAGVSELLRQAYDQIFNRRSDVIPIYFAFTRNETTAVSAAIEFLNTFVQQYIA
                     YRRDEPVVSEAPLTLQELIELAPAADLEWIEQLVQSYNRLRFSNDDKALVRFCLSAPQ
                     RIPAARGRAFVMLDGSQLAEYLNGVVVLGTEILRVFSRGSFSFVLAGLRRQILEAAHD
                     AESNFERLDILRLEQLAPTEATKLVEHVARRQQVATSEAARDLIVQQFAGSPFFINIY
                     LQAAREKRAALLSYLDCERLYVDELFGGHIHRHFADLLEEISPRLDTRHLLIRLLWEA
                     AAGEEQVSTFEIWRKRLHMTAAELEDVLHRLHVQEFINWDETTVEAGAGPQAWKDYLK
                     IRYRLDVMNEPRALVVADMLADSLKRAPHTMARHYKQIANAGLRDLISRFNCQQVPSV
                     LFDYQNFSAKYKGAEGEYIISGLQSETEFIKLPQTIHLASCSSFMSDMRQLCEEEHCL
                     VAHTFEEGNYSDAREVIWLAADIGSKLEVDLAVVRAWCERFENMAERLGFSRFQIWFI
                     SNEGFSEDASKLLERRKAFGSSRQQLELLASRVRDTVPSEVVQSAEPDEFLMVVPMGE
                     DNELIAANTAEQIAKRLTFRPEAINQIKTAIVEACINASEHSFSPDRKIYQRFRVESD
                     RLVITISSRGVVPANLNGANSGTEVKQAAEERRGWGLKLIRTLMDEVEFERVDDGTSL
                     RMTKYLRPQKGN"
     gene            complement(3815..>4900)
                     /locus_tag="VFH96_06350"
     CDS             complement(3815..>4900)
                     /locus_tag="VFH96_06350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET6973628.1"
                     /translation="TSPTEPDLQGEIQRLRRELEYRARFTNSLQHFLERISCADPAKT
                     YNSIVSNSQELLQAERASLMIFDETANELVLKAANGLPTDPASVSAVRVGEGVSGEVI
                     DTGKPVMVTDLRIAGRKAAPPERRYKTNSFISYPIMIGGRKVGVLNVTDKSGGGVYDE
                     VDLSLLEIIGPQVALALERAEWQERATEFQLMSITDSLTGLPNRRYLEERLSEELNRS
                     KRYDYPMSFLMIDIDDFKAYNDKNGHQAGDVALQITAHCLKGALRAVDIASRYGGEEF
                     CILLPQTGISEAGVIADRIRHRVSTTEFPHGKAQPLGQVTISVGVSTFSKNVDTSENI
                     IAAADRALYQAKSMGKDRVEFYGEHER"
BASE COUNT         1046 a         1401 c         1333 g         1120 t
ORIGIN      
        1 aatcagccag cgagacgatc ttcgcgccca tcggtatctg ctggtccgtc agcccgtccg
       61 gatagccacg cccgtcgaga cgctcgtgat gatgtttcgc cataaacgga atgtcagcgt
      121 aaggatggcg aatcggcgcg aggatctcgt agccggcgtt tggatgccgg ttcagctcga
      181 tcgagttctt tgcatcgatt ttgtaaggcg cgttgatgat gtcgcggtcg acgatcagtt
      241 tgccgatgtc gtgcaggtaa cccgcaatct gaatcccctc aacctcttct tcactccaac
      301 ccaattcacg cgcgatgatc tcgctgtagc ggccgacacg ttctgagtgg ccctgcgtgt
      361 acttgtcttt gatgtctatc gcagccgcga aagctcgcac cgtatcgcga taaatcgtgc
      421 gcagctcgtc atataacctg cgattctctt ccgcgcgctg ggccaactgc tcgagtaaac
      481 gatgcgtgtg aatgccgaca ccgatgtgac ggaccatcgc gcgaattgtc tcgaagtcct
      541 cgttggaaaa ctcttcaccg ctcgctttcc cgccgaggag gaccaacccg gtgacttcac
      601 cacgcaccac catcggcact acgagctcga tcccctcacc agcgagcttg tttcggtatt
      661 tgcccaaaaa gacatttgca ctcgatgtat tcctaaccag cgactcgcgg atctcgccac
      721 cgaggaacga ctccatctgc tcctcatcga tctcaaatcc atgcaacgcc cggtcagtca
      781 gtccccagat cgcaacgcaa cgcaatggtc caggcccgga gcactcgacg accgcaccgc
      841 gtctaagcgc gagcgttccc agcagcaggt gcatcgacgt ccgcaccatc tcggtgaagt
      901 cgccggtgcc ggcaatctcc tgcccgagat cggccagggc tccgaaagtg tggataagct
      961 tctgggtcga aggctccatg ttcagtggac tacgggtgta gtagtctcaa aatgtgaaag
     1021 attcagaagt gcttctcgtt cgtccttcgc gagggcaaca tggcgggtca gtccaagcat
     1081 ctcgaacagt tggactgttt gattgttgac atcggagaag acgatttgcg cgccgctctt
     1141 ttcggcgacg tcgatgatgc cgaggagaat tgagattccg atactattga cgagtttggt
     1201 gtcgctgaaa tcaataacga gggcgcgaca gccgcaattc agttgccggc gacactcgcg
     1261 ctcgatcttt tcaccagaca gcttgttcag gtagtcgcta gcgtaaacaa ccgcggtagt
     1321 gccaacacag tgcgagcgta ccaaccctcc actcgatgta tccacttgat tgctcccctc
     1381 ccttgggccg caaaacggac acaaccccac agatcaattg tgttacttac tgaggtgccg
     1441 ctaatgctag gttcttaatt gcctttttgc ggtctgagat acttggtcat gcgcaaacta
     1501 gtgccgtcat cgacccgttc aaactcgacc tcatccatga gtgttcggat gagcttgagt
     1561 ccccaaccgc gtctttcttc agccgcttgt ttgacttctg taccgctatt cgcgccgttt
     1621 agattggccg gcacgacccc tcgactcgat atagtgatga ctaatctatc actttccacg
     1681 cgaaaacgtt gataaatttt tcgatcgggg ctgaaactat gctcagatgc gtttatgcag
     1741 gcctcgacga tggccgtttt tatctgattg atggcctccg gacgaaaggt taaacgcttt
     1801 gcgatctgct cggcggtgtt ggctgcgatc agctcgttgt cctcgcccat cgggaccacc
     1861 atgagaaact catcgggttc tgcggattgt acaacctcac tcggtacggt atcgcgcaca
     1921 cgcgaggcga gtaactccaa ctgctggcgg ctcgagccaa acgccttgcg acgttccagc
     1981 aacttgcttg cgtcttcaga gaaaccctcg tttgaaatga accagatctg aaagcgactg
     2041 aaaccgagtc gctcggccat attctcgaaa cgttcgcacc aggcgcgcac gacggcgagg
     2101 tctacttcca gcttcgaccc gatgtcagcc gccagccaga tcacttcgcg cgcgtcagaa
     2161 taattccctt cctcaaacgt gtgcgcgacg agacagtgct cctcttcgca aagctgtcgc
     2221 atgtcgctca tgaagctgga gcagcttgcg aggtggattg tttgcgggag tttgatgaac
     2281 tcggtttcgg actgcagtcc ggaaataatg tactcgccct cggcgccttt gtacttcgcg
     2341 ctgaagttct gatagtcaaa aagaacagac ggcacctgct ggcaattgaa gcgcgagatc
     2401 aaatcgcgca ggcccgcgtt ggcgatctgt ttgtaatgac gggccatcgt gtgtggcgcg
     2461 cgcttgagag aatcggcgag catgtcagca acgaccagag cgcgcggctc gttcattacg
     2521 tcgagacgat agcgaatctt caagtagtct ttccatgctt gtggaccagc gccggcctcg
     2581 actgtcgttt cgtcccagtt aataaactcc tgtacatgca ggcgatgcag cacgtcttcg
     2641 agctccgctg cggtcatgtg caggcgtttg cgccagatct caaatgtcga tacctgctct
     2701 tcgccggctg cggcttccca caacaagcgg atcaggagat gtcttgtgtc gagtcgcggg
     2761 gagatctctt cgagtagatc tgcgaagtgt cgatgaatgt gaccgccgaa aagctcatca
     2821 acgtagagcc gctcgcaatc gagatacgag agcagcgcgg cgcgtttctc acgtgctgcc
     2881 tggaggtaaa tattgatgaa gaacggactg ccggcaaact gctgcacgat cagatcgcgc
     2941 gcggcttcgc tggtggcgac ctgctgcctg cgcgcgacgt gttcgaccag ttttgttgct
     3001 tcggttgggg cgagttgttc gagacgcagg atgtcgaggc gctcgaaatt actttccgca
     3061 tcgtgagccg cttcgaggat ctgacggcgc aaacccgcga gcacaaatga aaaactgccg
     3121 cgtgaaaata cgcggagtat ctcggttccc agtacgacta cgccgttcag atactcggcg
     3181 agctgcgagc cgtcgagcat cacgaaggcg cggccacggg cagccggaat tcgttgcggc
     3241 gcgctcagac agaatcgcac gagtgctttg tcgtcgttgc taaagcgcag gcggttgtac
     3301 gattggacca gttgctcgat ccactcgaga tccgcggcag gcgcgagctc gatgagctct
     3361 tgtagcgtca gtggcgcctc tgacacgacc ggctcgtcac gacggtaagc gatatattgc
     3421 tggacgaagg tgttgagaaa ctcaatcgcc gcgcttacgg cagtggtttc gttgcgcgtg
     3481 aaggcgaagt aaatcgggat gacgtccgag cgtcgattga aaatttgatc gtatgcctgt
     3541 cggaggagct cggagacacc ggctgaaggt tccatgagca acagcagccc gcgacccgca
     3601 ttggcacgtt ccaactgggc cataatgcgg tcgagctccg ccgcacgacc gacgaagtcc
     3661 tgctggacca cgcggccaag aacacggcgg aagttttcac tgcggctact cattcaacac
     3721 agttgttaga caggatttac aagatttcac aggatgaaca ggacaaatct tgttaatcct
     3781 gaaaacatct tgtaaatcgt gtctacagcc accttcacct ttcgtgctct ccgtagaact
     3841 caacccgatc tttccccatg ctcttggcct ggtacagcgc gcgatcggcc gccgcgatga
     3901 tgttctccga cgtatctacg tttttcgaaa acgtcgacac accaacactg atcgtcacct
     3961 gcccgagcgg ctgcgcttta ccgtgcggaa actccgttgt cgaaacgcga tgcctgatac
     4021 gatcggcaat cacaccagcc tcactaattc ccgtttgcgg caacaaaata caaaactctt
     4081 caccaccata acgcgaagcg atgtcgaccg cccgcagcgc gcccttcaaa caatgcgccg
     4141 tgatctgaag cgccacgtca ccggcctggt gaccgttctt gtcgttgtaa gccttgaaat
     4201 cgtcgatgtc gatcatcaga aaactcatcg gatagtcgta acgcttcgaa cgattcaact
     4261 cttccgacag acgctcttcg agataacgac ggttgggcag gcccgttagc gaatccgtga
     4321 tcgacatgag ctgaaactca gtcgcgcgtt cctgccattc agcacgctcc agcgcgagcg
     4381 cgacttgtgg tccaatgatc tcgagtagac tcagatcgac ttcgtcgtaa acgccgccgc
     4441 cggatttgtc agtcacattc aagacaccga cttttcgccc accgatcatg atcggatagc
     4501 tgatgaatga gtttgtttta tagcgtcgtt ctggtggcgc ggctttgcgg cctgcgatgc
     4561 gcaggtcagt gaccatcact ggcttgcccg tgtcgatgac ttctcccgaa actccctcgc
     4621 cgacacgaac agccgaaact gatgcgggat ctgtcgggag tccgttggct gccttgagga
     4681 ccaactcgtt cgcggtctcg tcaaagatca tgagcgaggc gcgctccgct tgcagcagct
     4741 cctgcgagtt gctgacgatc gagttgtaag tcttggctgg atcggcgcag cttattcgtt
     4801 cgaggaagtg ttgtagcgag ttagtgaaac gggcgcgata ttcgagctcg cgtcgcaggc
     4861 gttgaatttc gccctggaga tctggttcgg ttggggaggt
//
LOCUS       DASSFQ010000181         7675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1571483, whole genome shotgun sequence.
ACCESSION   DASSFQ010000181 DASSFQ010000000
VERSION     DASSFQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571483"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFH96_06355"
     CDS             complement(<1..1025)
                     /locus_tag="VFH96_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET6973629.1"
                     /translation="MKSDNSWLQTTTGIVVVLLAVQFLTGVLLAFYYVPSVDHAYTTV
                     SYIEKVVSSGSWIRSLHHYGSQWLTVFVFMHVIRLFLDRAYASNRIQWIAAILILGLV
                     MAAGGTGYSLPWDARAFFSTRVAEGLMAGLPIAGGMARLWLLGGSEISGVTLSRFFAL
                     HVLVIPAVIGAVVVWRMFREQIAEFLGPRDAIVGTLVFLVLALWALQHPAPFGPSVAE
                     AGADYLPRPGAQFLWLYQSLKYIPGGLGSIVGVVLPGIVLAVLVGLPWLKRQRLIAGS
                     LLGAGVVLVALMTTVSYFSDRRDPRTAQQFARQVAAEEAWRRQPFNPATITLAPATPA
                     NTTGGAPV"
     gene            complement(1022..1498)
                     /locus_tag="VFH96_06360"
     CDS             complement(1022..1498)
                     /locus_tag="VFH96_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET6973630.1"
                     /translation="MNIDTSRRSFLKFLPLAAVFVSIGGAAFRFLRPKLSAATNAWLD
                     VASVSELSGPQPISRKIVAEQITGWAITTEEHNVFVLPAKNNQVVSAICPHEGCEVAW
                     EQNRNRFSCPCHESYFAADGSRISGPAQRGLDALPARVQDGKLQVQYISREQATRA"
     gene            1690..2460
                     /locus_tag="VFH96_06365"
     CDS             1690..2460
                     /locus_tag="VFH96_06365"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET6973631.1"
                     /translation="MSSDKDPGNATRGSLRLAPAYAEVEVEKAKLPRQFVNFTFYKVR
                     PEWRLLADGDKQRGRDEFVKTVDEFRSSLLIHSYSTIGLRTNVDFMIWRIGYELDPIQ
                     EMTSQLNKTEMAKYIEPSQSFLSMTKRSMYIDKDNPEHVEDRLHIVPGKSQYLFVYPF
                     VKTREWYSKPPEQRQEMMDEHIRIGMKYRSVKLHTTYSFGLDDQEFVVAFETDYPSDF
                     LDLVQELRETKASSYTLRDTPMFTCRQRTLSECLEALG"
     gene            2477..5194
                     /locus_tag="VFH96_06370"
     CDS             2477..5194
                     /locus_tag="VFH96_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973632.1"
                     /translation="MTEIRKHLRNLWIVLFVLLSAATLYSQTDTPSEYLWYEAENMRG
                     FSTGKLGEPLLNPSHMNLSREKAPGWGISGPGVSAEWTQGGESEWNSAAASADETRAT
                     LYQDLEIPRGGEYRLWVRYADWAKRTENFTITIAQDGAEVFRHEFGAKDVIDPHDEVS
                     MYWGWAFAWDSAGATVKKGPARVSIVIEKAAEARRHVDCFLLTNDLAFKPSGRQKPDF
                     SAMRYMRTWSTTRKPLQALLPREEMTRVPEAWVRPKVGGRDFLMPWNIAKEFWQLLDK
                     PAEDRPLFPFNAEPIEEFVKKYKGTREVPLFQSNLVVPVIYINDLPEYLKEGSPFLRY
                     LRETKVPFAILINYGAAKLDPAAWQLLNGELKDQFLGLISGESVGYVWDSAPAELKIT
                     PSMSRRELLEAHRQFYSNAIARKWRETFQTETGPLWDKLIPAQSTSSTSFAHALTQWG
                     VNLIGMETAAVMPMTGMRIAFTRGAARQFGGAFLYYHAPNFGDTATTFTKQQNFAGPD
                     YFYHSRYGPTMGPSLAWYRKSYYLYYMAGASAIYLEQGQDQFFKPGPGEHPFQLNPLG
                     RITDEFMRFVAKHPERGTPYTPIAFLLDPAHGFEMTDYPQWPFEVSQIDRGDRALREL
                     FGVAYYPGLVVEGEPAMADRQPFVPGVFGDIFDVITAADVQGSRSKVQTLLPSYRAVV
                     VGGRIDWSNEWIARLNEYVRNGGTVVVNAAQIKKLPEQFLGVRLLNDTGEAHNAVCSA
                     PGDEAEYLKGQIFRYEKVELKGATALMTVPSGDPIVTVNKVGKGSVVFVAVSDLLGED
                     ERMTPFAVHLLAHVFADAVPVKVTGDVEYLINRTANGWVVTLLNNNGVFKPQQGMAHV
                     DRNAYVTATISVPQMKSAIEWTSESTLKPENGKATLQIPPGGLAIVEIR"
     gene            complement(5202..6824)
                     /locus_tag="VFH96_06375"
     CDS             complement(5202..6824)
                     /locus_tag="VFH96_06375"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET6973633.1"
                     /translation="MQPSRRVFLGQVGAATLAATVIGSEAQAANGANQRANECAKLRR
                     EAAMAGLQATPPNLQHPNNEDENLYPNKIGNYSKGLPHNNDGTVVLSAYNALVNAINS
                     SRPADFDAIPLGGTRGLTNPQAGLAFDMQGPDAHALVQPPAPALASREQAAEISENYW
                     MALLRDVPYSQYSTNPIANAAAADLTLYGADFKGPKSGGAVTTATLFRGLTPGDRVGP
                     QLSQFFYQDCNFGANKIEQKITTTVPGVNYMTDFNTWLAVQRGIAQGPDAFDPVPRYM
                     RNGRDIGQWVHIDVLFQAYFHAFLILAGAGAPFDNGNPYNNNPTQIGFGTFGGPHIAT
                     LLCEVSTRALKAVWFQKWFVHRRLRPEVMAERVDRTLFAGAGYPVHPEILNSFNDSNR
                     LKGFMPAGSALLPMAFPEGSPTHPAYGAGHATVAGACVTILKAWFKETTRLVDIGLDP
                     VQPTDDGLSLGPYTGSDTADITVGGELNKIAANVALGRNIAGVHWRSDATESLKLGEA
                     IAIGILKDQRACYNEVFNGFSLTKFDGSTIVV"
     gene            complement(6950..>7675)
                     /locus_tag="VFH96_06380"
     CDS             complement(6950..>7675)
                     /locus_tag="VFH96_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973634.1"
                     /translation="FTRGVVWRHARKADRNMLASVNPVLDPLLVSPLRNAVVHSAWGR
                     GKIDIPPGIARKLNDTTVVTSTTPVAPKFRPDLAKRMRVNNAPDHAKGQKFQVRDERQ
                     ERGRGGPPQQSAQAPAPVQQQQRPAQVEQRDRGQEQRGRGQEQREARRAEQPQQQRPQ
                     PQQQQPRGQQKQAERRASAPQAERPARPVRQEQPRGERVAAPARPQPQAQQPKHERPP
                     QAQKQQQPKQERGNPGKGKGKKP"
BASE COUNT         1594 a         2144 c         2251 g         1686 t
ORIGIN      
        1 actggcgcgc cgccagttgt atttgcaggc gtcgccggcg cgagtgtgat cgttgccgga
       61 ttgaacggct gacggcgcca cgcttcttcc gctgctactt gtcgagcgaa ttgttgggcc
      121 gttcgcggat cacgccggtc actgaaataa gacactgtgg tcatgagcgc gaccagaacg
      181 accccagcgc cgagtaacga accagcaatc aatctttgcc gtttgagcca tggcaatcca
      241 accaaaacgg ccaggacgat tcccggcagg accaccccca cgatcgatcc gagtccgccc
      301 ggaatgtact tcaacgattg atacaaccac aaaaactgcg cgcccggtcg cggcaaatag
      361 tctgcacccg cttctgcgac tgatggacca aacggcgccg gatgctgcaa tgcccaaagc
      421 gcgagcacga gaaacacgag cgttcccacg atcgcgtcgc gtggtccaag aaactccgcg
      481 atttgctccc tgaacattcg ccagaccaca accgcaccga tcaccgccgg aatcacgagc
      541 acgtgaagcg caaagaatcg cgagagtgtc acccccgaaa tctcgctgcc gccaagcaac
      601 cagagccgcg ccattcctcc cgcaatcgga agcccggcca tcaaaccttc cgctaccctc
      661 gtactgaaaa acgcgcgcgc gtcccacggc agtgaatagc ccgtgccgcc cgctgccatc
      721 acgagcccga gaatcaagat cgcagcgatc cattgaatcc tgttcgacgc ataagcgcgg
      781 tccaaaaaca gccggatcac atgcatgaaa acaaagacgg tgagccactg tgagccgtag
      841 tgatgcagcg agcgaatcca cgaacccgac gacacgactt tctcgatgta cgacacagtc
      901 gtgtatgcat gatcgacaga aggcacgtaa taaaacgcca gcaaaactcc cgtcagaaac
      961 tgaaccgcaa ggagcacaac cacgatgccg gtcgtcgtct gtaaccacga gttatcgctt
     1021 ttcatgcgcg tgtcgcctgt tccctggaga tgtattgcac ttgcagtttt ccatcctgca
     1081 cacgcgccgg caacgcgtcg agcccgcgct gagctggacc actgatacgc gagccgtcgg
     1141 cggcgaagta gctctcatga cacggacacg aaaaacgatt cctgttttgt tcccacgcga
     1201 cttcacagcc ttcgtgagga cagattgccg ataccacctg attattcttc gcgggcagga
     1261 caaacacgtt gtgctcttca gtcgtgatgg cccagcccgt gatctgctcg gcaacgatct
     1321 tgcggctgat tggctgtgga ccactcagct ccgaaactga ggcgacatcg agccaggcat
     1381 tcgtcgcggc ggagagtttt gggcgcagaa agcgaaatgc ggcgccgccg atcgatacga
     1441 agaccgcggc cagcggcaga aacttgagaa atgagcgacg cgatgtgtcg atgttcatgc
     1501 gtttacctcg tcacctttga cgctggagcg cccggctttc ttccgcgtag ttagacaggg
     1561 tttcaggatt ttcaagattg acacgattct ccccgtggtt gcattcggct ctgagactgg
     1621 gcagaataac tttgcatcgc tgattcttcc aaggttagtc atccgtaatt cagacctgag
     1681 gtaaaaacaa tgagtagcga taaggatcca ggtaatgcca cgcgtggctc gcttcgactg
     1741 gcccctgcct acgccgaagt cgaagtggaa aaagccaagc tgccccgaca gtttgttaac
     1801 ttcacgttct ataaagtgcg tccggagtgg cgtctgctcg ctgacggcga caagcagcgc
     1861 ggtcgtgatg agtttgttaa gactgtcgat gagtttcggt cctcgttgct gattcattct
     1921 tactcaacta tcggacttcg tacgaatgtc gacttcatga tctggcgcat cggctacgag
     1981 ctcgatccga tccaggagat gacctcgcaa ctcaacaaaa ctgagatggc caaatacatc
     2041 gagccgtcgc agtcgttcct ctcgatgacg aagcgctcga tgtacatcga caaagataat
     2101 cccgagcacg ttgaagatcg gctgcacatc gttcccggca agtcgcagta tctcttcgtt
     2161 tatccattcg tgaaaactcg cgagtggtat tcaaagcctc cggagcaacg ccaggaaatg
     2221 atggacgagc acatccgcat cggcatgaag tatcgctcgg taaaactgca caccacgtac
     2281 tcgttcggcc tcgacgatca ggagtttgtc gttgcgtttg aaaccgacta cccgtctgac
     2341 tttctcgacc tggtccagga gctacgcgaa acaaaagcca gctcctacac gctgcgcgac
     2401 acaccgatgt tcacttgccg ccagcgaacg ctcagcgagt gtctcgaagc tttaggctaa
     2461 gccacagatt acacagatga cagagattag aaagcatctg cgtaatctgt ggatagttct
     2521 gtttgttcta ctgtccgccg cgactcttta ttcccaaacc gatacgcctt cggagtatct
     2581 ctggtacgaa gccgagaaca tgcgcgggtt cagcaccggc aagctcggtg agccgctgct
     2641 taatccgtcg cacatgaatc tgtcgcgtga gaaagcgccg ggttggggaa tcagtgggcc
     2701 gggagtttca gcggagtgga cgcagggcgg tgagagcgaa tggaactctg ccgccgcgag
     2761 cgctgatgag acgcgtgcga ctttgtatca ggatctcgag attccacgtg gcggcgaata
     2821 ccggttgtgg gttcgttacg ctgactgggc gaagcgaact gaaaacttca cgatcacgat
     2881 cgcgcaggat ggcgctgaag tgtttcgaca cgagtttggc gcgaaagatg tgatcgatcc
     2941 acacgacgaa gtcagcatgt actggggctg ggccttcgcg tgggacagtg caggggcgac
     3001 ggtgaagaaa ggccccgcgc gtgtgtcgat cgttatcgag aaagccgcgg aagcgcgaag
     3061 gcacgttgac tgtttcctgc tcacgaatga tctggcgttc aagccgtcgg gtcgacaaaa
     3121 gcctgacttc tcagcgatgc gttatatgcg cacgtggtcc acgacgcgca agccgttgca
     3181 agcgctactg ccacgtgaag aaatgacgcg cgtgccggaa gcgtgggtgc gaccgaaggt
     3241 tggtggtcgc gacttcctga tgccgtggaa catcgcgaag gagttctggc agctgctcga
     3301 taaacctgcc gaagaccgcc cgctgtttcc attcaatgcc gaaccgattg aagagttcgt
     3361 caagaagtac aaaggcacgc gcgaggtgcc gctctttcaa tcgaatctcg tggtgccggt
     3421 tatttacatc aacgacctgc ctgagtactt aaaagagggc agcccgtttc tgcgttacct
     3481 gcgcgagacg aaagttccct ttgcgatcct tatcaactac ggcgcggcga agctcgatcc
     3541 ggcagcatgg caactgctca acggcgagtt gaaggaccag ttcctgggtt tgatctcggg
     3601 tgagagtgtc ggctacgtgt gggactccgc acccgctgag ttgaagatca caccgtcgat
     3661 gtcgcgccgt gagttacttg aagcgcaccg gcagttttac tcgaacgcga tcgcgcggaa
     3721 gtggcgcgag acgtttcaaa ccgagaccgg tccgctgtgg gacaagctga ttcccgcgca
     3781 gtcgacgtcg agcacttcgt ttgcacatgc gttgacgcag tggggcgtga atctgatcgg
     3841 catggaaacc gctgcggtga tgccgatgac gggaatgcgc atcgctttca cgcgcggcgc
     3901 tgcgcgccag tttggcggcg cgtttctcta ttaccacgcg ccaaacttcg gcgacaccgc
     3961 tacgactttc acgaagcagc aaaacttcgc cggcccggat tacttttacc attcgcgcta
     4021 tggtccaacg atggggccgt cgctcgcctg gtatcgcaag agttattacc tctattacat
     4081 ggctggggct tcggcgatct atctcgagca gggccaggac cagttcttca aaccgggacc
     4141 gggcgagcat ccgttccaac tcaacccgct cggccgcatc accgacgagt ttatgcgttt
     4201 cgttgcaaag catccggagc gcggaacgcc gtacacgcct atcgctttcc tgctcgatcc
     4261 ggcacacggg tttgaaatga cggattaccc gcaatggccg tttgaagtgt cgcagataga
     4321 tcgaggggat cgggcgctga gagagttgtt tggcgtggct tactatccgg gactcgtggt
     4381 tgaaggtgag cctgcgatgg ccgaccggca gccgtttgtg cctggagttt tcggcgacat
     4441 attcgatgtg atcactgcgg ctgatgttca gggatcgaga tcgaaagtgc agacgctctt
     4501 gccgtcgtat cgcgcggttg ttgttggcgg acgtatcgat tggtccaacg agtggattgc
     4561 aagactaaat gagtatgtgc gaaacggcgg tactgtcgtc gtcaacgcgg cgcagatcaa
     4621 aaagctgccc gagcagtttt taggtgtgcg attgctcaat gacactggtg aagctcacaa
     4681 cgctgtttgc tccgcgcccg gcgacgaagc tgaatatctc aaaggccaga tattccggta
     4741 tgagaaagtc gagctaaagg gtgcgacggc gctgatgacc gtgccgagtg gtgatccgat
     4801 cgtgacggtg aataaagtcg ggaagggaag cgtcgtgttt gtggcagtct ctgatttgct
     4861 cggcgaagat gaacgcatga cgccgttcgc cgtgcacctg ctcgcgcacg tgtttgctga
     4921 tgctgtgccg gtgaaggtaa caggtgatgt tgagtatctg atcaatcgaa cggcgaacgg
     4981 ctgggtggtg acgttgctga ataacaacgg cgtcttcaag ccgcagcagg gaatggccca
     5041 cgtcgatcgg aatgcttatg tgacggcgac gatctctgta ccgcagatga agagcgcgat
     5101 tgaatggact agtgaatcga ctttgaaacc tgaaaatgga aaggccaccc ttcaaattcc
     5161 accgggtggc ctcgccattg tcgaaatccg gtaacgcgga tttaaaccac gattgttgag
     5221 ccgtcgaatt tagttagact gaagccgttg aagacttcgt tgtagcaagc tcgctggtcc
     5281 ttcaaaattc cgattgcgat tgcctcaccg agtttcagcg actcagtggc gtcagagcgc
     5341 cagtggacac cggctatgtt tcttccgagg gcgacgtttg ccgcgatctt gtttagctca
     5401 ccgccgacgg tgatgtcggc tgtgtcggaa cctgtatacg gtccgagaga caggccatcg
     5461 tcggttggtt gcactggatc gagaccgatg tcgaccagtc gcgttgtctc cttgaaccac
     5521 gccttcagaa tcgtgacgca cgcgcccgcg acggttgcgt gtccggcacc gtaagcggga
     5581 tgtgtcggcg agccttccgg aaacgccatc ggcaggagcg cgctgcccgc cggcatgaat
     5641 cccttcagac gattcgaatc gttgaacgaa ttgagtatct ccggatgcac cggataaccc
     5701 gcacccgcaa aaagcgtccg gtcaactcgc tccgccatca cttccggtcg caggcgtcga
     5761 tgcacgaacc acttctgaaa ccacacggcc ttcaacgcgc gggttgagac ttcgcacagg
     5821 agcgtcgcga tgtgtggtcc accaaaagtg ccgaacccga tctgagtcgg attgttgttg
     5881 taagggttgc cgttatcgaa cggcgcgcct gcgccggcga gaatcaggaa cgcatggaag
     5941 tacgcctgga acaacacgtc gatatgtacc cactgtccga tgtcgcgacc attacgcatg
     6001 taacgcggca ccggatcgaa tgcgtccggg ccttgcgcga tgccgcgctg gactgcgagc
     6061 cacgtgttga agtcagtcat gtagttgacg ccaggaacag tggttgtgat cttttgctcg
     6121 atcttgttcg cgccgaagtt gcagtcctgg tagaagaatt gagagagctg tggtccaacg
     6181 cgatcgccgg gcgtgagacc gcgaaataga gtggcagttg taactgcgcc gccactcttc
     6241 gggcctttga agtccgcgcc gtacagcgtc agatcggcag cggcagcatt tgcgatcgga
     6301 ttcgttgagt attgtgagta gggaacgtcg cgcagtaacg ccatccagta gttttccgaa
     6361 atctctgcgg cttgttcacg actcgcaaga gcgggcgctg gtggttgtac gagtgcgtgc
     6421 gcgtcgggac cttgcatgtc gaaagcgagt ccggcttgag gattggttag ccctcgcgtg
     6481 ccgccgagtg gaatggcgtc gaagtctgcc ggtcggctgc tgttaatcgc gttgacgaga
     6541 gcgttgtaag cgctgagcac gacagtgccg tcgttgttgt gcggcaggcc cttcgagtag
     6601 ttgccgattt tgtttgggta gaggttttcg tcctcgttgt ttggatgttg caggttcggt
     6661 ggtgtcgctt gcaggcccgc catcgccgct tcgcgacgga gcttcgcgca ctcattcgcg
     6721 cgttgattgg cgccgttggc tgcttgagct tcggagccga ttacagttgc ggcaagggtg
     6781 gcagcgccca cttgaccgag gaatacacgt ctgctgggtt gcatggagga acttcctttt
     6841 ctctgggatg tgtcaggggc gggtattcta cacaggtgag gcacagatta cacggatttt
     6901 aaatccggat ttaatccgga tctaatccgt gtaatccgcg ttacgggttt tatggtttct
     6961 tgcctttacc ctttccagga ttaccgcgct cctgcttagg ctgttgttgc ttctgcgcct
     7021 gcggtggacg ctcgtgtttc ggttgctgcg cctgcggttg agggcgcgct ggtgctgcga
     7081 cacgctcgcc tcgtggctgt tcctgtctca ccggtcgcgc gggtcgttcc gcctgaggcg
     7141 cactggcccg tcgctcagcc tgcttctgtt gaccgcgcgg ctgctgttgc tgcggttgcg
     7201 gtctttgctg ctgcggctgc tcagcgcgtc gagcttcacg ttgttcctga ccgcgacctc
     7261 tctgctcctg gccacgatct ctctgttcaa cctgagcagg tcgctgctgt tgttgcaccg
     7321 gagccggagc ttgcgctgac tgctgtggtg gtccaccacg tccgcgttcc tggcgctcat
     7381 cacgcacctg gaatttctgt cccttcgcat gatcgggtgc attgttcaca cgcatccgct
     7441 tcgccagatc tggccggaac ttcggtgcga ccggagtcgt gctcgtaacg acggtcgtgt
     7501 cgttcagctt ccgtgcgatt cccggcggga tgtcgatctt gccgcgaccc catgccgagt
     7561 gcaccactgc attccgcaaa ggcgacacca gcaacggatc caacaccgga ttcactgacg
     7621 cgagcatatt tcgatcggcc ttccgcgcgt gtctccaaac gacgccacgt gtgaa
//
LOCUS       DASSFQ010000182         8092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1571719, whole genome shotgun sequence.
ACCESSION   DASSFQ010000182 DASSFQ010000000
VERSION     DASSFQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571719"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFH96_06385"
     CDS             complement(<1..634)
                     /locus_tag="VFH96_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET6973635.1"
                     /translation="MRRVGFVCAVLVLSGVAAMGQAPAAPATDCGELKTRLDRAETRL
                     SDWPALARYREDNKKTMAPAKGEKRVVFMGDSITDSWDSPSNGGFFPGKPFVNRGISG
                     QTTPQMLIRFRRDVIELKPKVVVILAGTNDIAGNTGPMTLEAIEDNLTSMAELATANG
                     IRVVFASLLPVSDYEVRDGKPIVQTMRRPPEKILELNKWMKAFAAVHHMIY"
     gene            complement(688..1623)
                     /locus_tag="VFH96_06390"
     CDS             complement(688..1623)
                     /locus_tag="VFH96_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973636.1"
                     /translation="MNRLGPVLGLLLVIGVFSLLVDTPSRLLSPNNLRIVLSQTVIVA
                     IGAIGMTMIIISGGIDLAVGSTIALTGVVTALAINAGFPPSVALLSGIMVGGLVGMVN
                     GLVITRLKVVPFIATLGMLGVARGVAKWLASQQTVNMPGTWLNELLVMFPTPAWLLVA
                     PGVWIAIVLAVFAAIVLRNTVFGRRVFALGSNEAAARACGIATDRLKIWIYGLAGLLF
                     GLAGVMQMSRLRQGDPTVAAGSELDIIAAVVIGGGSLNGGEGSILGSMIGALIMAFLR
                     NGCQQVGWPNYIQEIIIGSIIVIAVAIDRWRSSRA"
     gene            complement(1684..2682)
                     /locus_tag="VFH96_06395"
     CDS             complement(1684..2682)
                     /locus_tag="VFH96_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6973637.1"
                     /translation="MRKLLLLVLALTLVGACTKSNSPANNGKKKLTIAVIPKGTTHEF
                     WKSIHAGSIKAANELTAQGTEVEVIWKGPLREDDREQQIQVVEGFAAQGVSGIVLAPL
                     DDRALVRPVSDAGRAGVPTVIIDSGLQSNDYVSFVATDNRKGGSLAADRMGQLLNGKG
                     KVLVLRYAEGSASTTEREAGFIDQIKAKFPEIELVSTDQYAGATRDTAKRASENLLNR
                     FGDVVQGIFTPNESSTAGMLLALQDIGKAGKISFVGFDSSQTFIDAINANQLHGIVVQ
                     NPFNMGYLGVRTMVDSLMGKQVEKKIDTGVMLVTKENLETPDVKTLLHPPLDQYLH"
     gene            complement(2712..3644)
                     /locus_tag="VFH96_06400"
     CDS             complement(2712..3644)
                     /locus_tag="VFH96_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6973638.1"
                     /translation="MHIISFATEDSNWRSPRMGIILHVGDRDTGYRLDCEKLFDKADR
                     PANPLFWFDMDGPWFQKARETHDALVRDENALADANEKGWLVPSSDAYWFAPVPRPGK
                     IICIGLNYRDHAAESNMAIPEKPIMFSKFSTCVIAPGEPVVVPSTSQQVDYEAELAVV
                     IGRRAKHVSADNAYDYVLGYTAFNDVSARDFQFADGQWQRGKSCDTFAPMGQTIVTTD
                     AIKDPHKLSIKLVLNGQTMQDSNTDQLIFGVPALIEHITQSITLEPGDVIATGTPPGV
                     GFARKPPVFLKAGDQMEVLIEGMGGLGNPVVNDE"
     gene            complement(3644..4300)
                     /locus_tag="VFH96_06405"
     CDS             complement(3644..4300)
                     /locus_tag="VFH96_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HET6973639.1"
                     /translation="MNNSARETILGSIRSHLAASVPYDKQEDSRGFTRMNADQNPSSS
                     VEICGQFKASLEAVDGHCVIVRGDNEIAQALTNIIQGGRIAISDNPEVERLLQLTDLE
                     IEELGIAPSAHDIFRFDVGISTVQAAIAETGTLVLDSALERHRFVSLVPPVHIAIVRA
                     SQIYRTLSEVLSLIRKDQEVSPAVTFITGPSRTADIELTLAIGVHGPQELYVIIDEVN
                     "
     gene            complement(4293..5663)
                     /locus_tag="VFH96_06410"
     CDS             complement(4293..5663)
                     /locus_tag="VFH96_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HET6973640.1"
                     /translation="MNHLSSETFDQNAKAALADSHLRGALKNATSLFGERRKAAADSL
                     PNWEELRSEARAIKDEVLLHLDRYLLEFVGNAESRGAKIHWARDAAEANSIICGLALE
                     RQARTVVKSKSMTTEETHLNDALEAAGMQVVETDLGEYIIQLAEEPPSHIIAPAIHKT
                     RGQIAELFNAELGMPLTDDIARLTSTARATLRDRFAEADVGISGVNFAIAETGTIVIV
                     ENEGNIRLTTSLPRIHIAVMGIEKVLPRFADLDVFLKLLPRSGTGQRLTTYQSYITGT
                     KGQPTDEGPEELHVVLLDNGRSRMLAHPVTRQSLACIRCGACLNACPVYQQVGGHAYG
                     SVYPGPIGAVITPQLMGIQKAAQLPFASSLCGACREVCPVKIDIPRLLLHLRAEISPS
                     KGKLSERLAFKMWAQVMMRPSLYKLSSVVGRVMQRFFPVSKAWTSGRDLRPIESKSFR
                     ELWSDE"
     gene            complement(5676..6407)
                     /locus_tag="VFH96_06415"
     CDS             complement(5676..6407)
                     /locus_tag="VFH96_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET6973641.1"
                     /translation="MKVSLFVTCLVDQLWTSVGVSSVEVLRRVGCEVEFDKRQTCCAQ
                     PAFNTGYLDEARPVARRFIEIFEESKADAIVSPSGSCTAMVHHYPQLFAGDPEWLPRA
                     QAVAARTHELSSFLVRVLKVEDVGASWQGRLTWHDACHGLRDLNLKSEPRKLIRNVRG
                     AEFVELEHADSCCGFGGTFSVKYPEISVAILDQKIDAIERAGVNAVVSGDASCLMQIG
                     GRLSRKGSKVKVMHLAELLAADTRG"
     gene            complement(6453..7631)
                     /locus_tag="VFH96_06420"
     CDS             complement(6453..7631)
                     /locus_tag="VFH96_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6973642.1"
                     /translation="MESQTMKASVLCDVNRLEVRDIPRPVISPYEVLIRVAAVGLCGT
                     DVHIFAGHANYNTNEYGQAIPLALQPQILGHEITGVVEQVGSAVRDLSEGDRVVVDQG
                     LNCVSRRREGLCDYCGTENSHQCEFYREHGITGLPGGLAEFIAVPAVNAVKITMPLDA
                     SIAALVEPLGCIIHSSDTVAKARTRHAVRAESAERRVRSVLICGAGPAGLLFTQYLRN
                     VLLYEGWLFVSDPNERKRQLARQLGADEAIDPVTSDLVEVVHEHTGGKGVEYLIEASG
                     SGDVFRVMPGLIRKQATVLCYGHGHAGTDLSVLSSIFFKEPVLVSSVGASGGFESDGR
                     PSVYTRALKLIEQKQIDIAPLITHRYTSFEEVEKALSTDIHAADYVKGVVVLAADLRG
                     "
     gene            complement(7659..>8092)
                     /locus_tag="VFH96_06425"
     CDS             complement(7659..>8092)
                     /locus_tag="VFH96_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973643.1"
                     /translation="LTVYGVNRDTGLTSDPTKAMKSAVLGRPFHIRFGGATDFQYVAD
                     TAAAFLACADNAPEGAHVFNLHGETVTVDRIANFINSVSGDLITFGGPPIPIAAAMDD
                     SAIRNTIGALPSTPLETGIRETMDRFAALRDADRLDTSDLI"
BASE COUNT         1883 a         2503 c         1949 g         1757 t
ORIGIN      
        1 ggtagatcat gtgatgcaca gcggcaaacg ctttcatcca tttgttcaac tccaggatct
       61 tctccggagg ccggcgcatt gtctgcacaa tcggcttacc atcgcgaacc tcgtaatcac
      121 tcaccggcaa gagcgatgca aacacgacgc gaataccgtt tgcggtagcg agctcagcca
      181 ttgaagttag attatcttca atcgcttcga gcgtcattgg accagtgttg ccggcaatat
      241 cgtttgtacc ggcgagtatc accactacct tcggcttgag ttcgatcaca tcacgacgaa
      301 agcgaatcag catctgcggc gtcgtttgcc cgctgatccc ccgattgaca aaaggcttgc
      361 ccggaaaaaa cccaccattg ctcggactgt cccacgaatc ggtgatcgaa tcacccataa
      421 acaccacgcg cttctcgccc ttcgccggag ccattgtctt cttattatcc tcacgataac
      481 gcgccagcgc cggccaatca ctcaaccttg tctccgcccg atcaagcctt gtcttcaact
      541 ccccacaatc agtcgcaggc gctgccggtg cttgtcccat tgctgcaact cccgacaaca
      601 ccaacacagc acaaacaaaa ccaactctcc tcatctcttc tccttttgtg cttttgtgcc
      661 tttttgtggc taaatttttc tttgtggcta agcccggctt gatctccagc gatcgatcgc
      721 gaccgcaatg acgatgatcg acccgatgat gatctcctga atgtagttgg gccaaccaac
      781 ctgctgacag ccgttgcgca ggaacgccat gatcagcgcc ccgatcatag atccgagaat
      841 gcttccctcg ccgccattca aactgccgcc accaatcacc accgcggcga tgatgtccaa
      901 ctccgatccg gccgccacag ttgggtcacc ttgtcgcaaa cgcgacatct gcatcactcc
      961 ggcaagtccg aacagcaatc ccgccaatcc gtaaatccag atcttcaacc tgtcagtcgc
     1021 aatcccacac gcacgcgccg ccgcttcatt cgacccaagc gcaaaaacac gtcgtccaaa
     1081 aaccgtgtta cgcaaaacga tcgcggcaaa aaccgccagg actatcgcaa tccacacacc
     1141 aggcgcgacc agtaaccacg ccggcgtcgg aaacatcact agcaactcat tcaaccaagt
     1201 gcctggcatg ttcactgtct gctggctcgc caaccactta gccacgccgc gcgcgacacc
     1261 gagcattccc agcgtggcga taaacggcac gactttgagg cgcgtgatca caagaccatt
     1321 caccatgccg acgagcccgc ccaccatgat tccactcaac aaagcgactg aaggtggaaa
     1381 cccggcattg attgcgagcg ccgtgacaac tccggtcaac gcgatcgtcg atccaaccgc
     1441 aagatcgatt ccgccactga tgatgatcat cgtcatcccg atcgcgccga tggcgacaat
     1501 cactgtttgc gaaaggacga tgcggagatt gttcggtgaa agaaggcgcg aaggcgtgtc
     1561 aaccagaagt gaaaagacac caatcaccaa cagcaacccg agcaccggcc caagtcgatt
     1621 caaccattga tggaggagac gcgccgcaac cataatccgg attttatccg tgcaatccgc
     1681 ggtttaatgt aagtattggt ccagtggcgg atggaggagt gtcttaacgt ccggtgtttc
     1741 gagattctct ttcgtcacaa gcatcacacc cgtgtcgatc ttcttctcga cttgcttacc
     1801 catcaaacta tcgaccatcg tccgcacgcc gagataaccc atattgaatg gattctgcac
     1861 cacgatccca tgtaactggt tcgcattgat cgcgtcgatg aacgtctgac tcgagtcgaa
     1921 accgacaaac gaaatctttc cagccttgcc gatgtcctgc agcgccaaaa gcatgccggc
     1981 agtcgacgat tcattcggcg tgaagatccc ctgcacgacg tcgccaaaac gattcaacaa
     2041 attctccgac gcgcgtttcg ccgtgtcgcg cgtggcgccg gcgtattgat cggtcgaaac
     2101 taactcgatc tccggaaact tcgctttgat ttgatcgata aaaccggctt cacgctcggt
     2161 cgtgctcgcc gaaccttccg cgtatctcaa gaccagcacc ttgcctttgc cattcaagag
     2221 ttggcccata cgatcggcgg caagcgaacc gcctttacga ttgtcagtcg ccacgaagct
     2281 aacatagtca ttcgactgca aacccgaatc gatgatcacg gtgggcacac cggcgcgacc
     2341 agcgtcggac actggacgaa caagcgcgcg atcgtcgagt ggcgccagca cgatgccact
     2401 cacaccctgc gcggcaaagc cttcgacaac ctgaatctgc tgttcgcgat catcctcgcg
     2461 caacgggcct ttccagatca cttcgacctc tgtgccctgc gcggtgagtt catttgcagc
     2521 cttaatgctg ccggcgtgaa tgctcttcca aaactcatgc gtggtgcctt tagggatgac
     2581 ggcgatcgtg agtttcttct tgccgttgtt tgccgggctg ttagatttcg tgcaagcacc
     2641 gactagcgtc agtgcgagta ctaacaaaag aagtttgcgc atgtgatttt tgtgcctctt
     2701 cgtggccaat actattcgtc attaacaacc ggattaccca aaccacccat gccctcgatc
     2761 aacacttcca tttgatcccc agccttgaga aaaaccggcg gcttgcgtgc gaagccaaca
     2821 cctggcggcg tacccgtcgc tatcacgtca ccgggttcaa gcgtgatcga ctgtgtgatg
     2881 tgttcgatca acgccggcac accgaagatc agttggtccg tgttcgagtc ctgcatcgtt
     2941 tgaccgttca gcaccagctt aatcgagagc ttatgcgggt ccttgatcgc gtcggtagtc
     3001 acgatcgtct ggcccatcgg agcaaacgtg tcacacgatt ttccacgttg ccactgtcca
     3061 tccgcaaact gaaaatcacg cgcgctcacg tcgttgaaag cggtgtagcc caacacgtaa
     3121 tcgtaagcat tatcggcgct gacatgcttt gcacgccgtc cgatcacgac cgcaagctcc
     3181 gcttcgtaat caacctgttg cgaagtcgac ggcactacca ccggctcacc gggcgcaatc
     3241 acgcaagtcg aaaacttcga aaacatgatg ggcttttcgg gaatcgccat gttgctttcg
     3301 gctgcgtgat ctcggtagtt caggccgatg cagattatct ttccgggtcg cggcacgggc
     3361 gcgaaccagt aagcgtcgct gcttggaacc agccagcctt tttcgtttgc atcggcgagc
     3421 gcgttttcat cgcgcactag tgcgtcgtga gtttcgcgcg ctttttgaaa ccatggacca
     3481 tccatgtcaa accagaagag cggatttgcc ggacgatcgg ctttgtcgaa caacttctcg
     3541 cagtcgagac gatagccagt gtcgcgatcg ccaacgtgta aaatgattcc catgcgtggc
     3601 gagcgccagt ttgagtcttc agttgcaaaa gagatgatgt gcattagttc acttcgtcga
     3661 tgatgacata aagctcttgc ggtccatgca ctccaatcgc gagtgtcaac tcgatgtcgg
     3721 cagtgcgcga tggtccagtg ataaatgtaa cggccggact aacttcttga tctttgcgaa
     3781 tgagactgag aacttcgctt aaggtccgat aaatctgcga ggctcgcacg atcgctatgt
     3841 gcacgggcgg cacgagcgaa acgaatcgat ggcgttcaag cgcactgtcg agcaccaagg
     3901 ttcctgtctc cgcaatcgcc gcctgcaccg tgctgatgcc gacgtcaaat cgaaaaatgt
     3961 cgtgagcgct cggcgcaata ccgagttctt caatctcgag atccgtgagc tgaaggagac
     4021 gctcaacttc cggattatca gagatcgcga tgcgtcctcc ctgaatgata ttcgttaacg
     4081 cttgggcaat ttcattatca ccccgaacga tcacgcagtg cccgtcgacc gcctcgagac
     4141 tcgccttaaa ctggccacag atctccactg atgaagacgg attctgatcc gcgttcatcc
     4201 gcgtaaatcc gcggctatct tcctgcttgt cgtaaggcac gctcgccgcc agatgcgatc
     4261 gaatagatcc gagaatggtt tcgcgcgctg agttattcat cggaccacaa ctctcgaaaa
     4321 ctctttgatt cgattggacg caggtcgcga ccgctggtcc atgccttcga cacgggaaag
     4381 aatcgctgca ttaccctgcc cacgaccgag ctcagcttgt aaagcgatgg ccgcatcata
     4441 acctgagccc acatcttgaa cgccagccgc tcgctgagtt tgcctttgct tggagaaatc
     4501 tcggcgcgca ggtgtaacaa gagacgtgga atatcaatct tcaccggaca aacttcccgg
     4561 caggctccac acaaactcga agcaaacggg agctgcgcgg ctttttgaat tcccatcaac
     4621 tggggcgtga ttaccgcgcc gatcggaccg ggataaaccg agccatacgc atgaccacca
     4681 acctgctggt aaaccggaca cgcattcaaa caagccccgc aacgaataca tgccagcgac
     4741 tgtcgtgtta ccggatgggc caacatacgc gagcgaccat tatcgagcag cacgacatgc
     4801 aattcttccg gaccttcatc tgttggctgt ccttttgtac cggtgatgta cgactgatac
     4861 gtcgtcagtc tttgacccgt gcccgagcgt ggcaggagtt tgagaaacac atcgaggtct
     4921 gcaaagcgcg gcaaaacttt ctcgataccc attaccgcga tgtgaatacg cggcaggctc
     4981 gtggtcaaac gaatattgcc ttcattctcg acgatcacga tcgttccggt ttcggcaatc
     5041 gcaaaattta cgccgctaat gccgacgtcg gcctccgcaa aacgatcgcg caaagtggcg
     5101 cgcgccgtgc tcgtaagacg cgctatgtcg tcggtaagcg gcattccgag ttcggcattg
     5161 aacaactctg caatctgccc gcgtgttttg tgaatggccg gcgcgatgat gtgcgatggt
     5221 ggttcttccg caagctgaat gatgtactca ccgagatcgg tttcgaccac ctgcatcccc
     5281 gccgcttcga gcgcatcgtt gagatgcgtc tcctcggtcg tcatgctctt gcttttgacg
     5341 acggttcgag cctgacgctc caacgccaac ccacaaataa tcgagtttgc ttctgccgca
     5401 tcgcgtgccc aatgaatctt tgcgccacgc gactcagcat taccaacaaa ctccaacaga
     5461 tatcgatcga gatgcaacaa cacctcgtct ttgatcgcac gcgcctcgct gcgtaactct
     5521 tcccaattcg gcaaactgtc cgccgccgct ttgcgccgtt cgccaaacag cgacgtcgca
     5581 ttcttcaacg ccccacgcag atgtgaatca gccagcgcgg ccttcgcatt ctgatcaaat
     5641 gtttcgctgc ttaaatgatt cattccgatc cgcgtttatc cgcgagtatc cgcggccaat
     5701 aactctgcca aatgcatcac cttcaccttc gatcccttgc gcgacaaccg gccaccaatc
     5761 tgcatcaaac aactcgcatc gccagacacc accgcattga cacccgcacg ctcgatcgcg
     5821 tcgatcttct ggtccaggat cgcaaccgaa atctccggat acttcaccga gaatgtccca
     5881 ccaaaaccac aacacgaatc cgcatgctcg agttcgacaa actcagcccc acgcacattc
     5941 cgaattaact ttctcggctc tgatttgaga ttgagatcgc gcaaaccatg acacgcgtca
     6001 tgccaggtta gcctgccttg ccacgacgct ccgacgtctt caactttgag cacacgaacc
     6061 agaaacgagc tcagctcgtg tgttcgcgct gctacggcct gcgctcgcgg gagccactct
     6121 ggatcaccgg caaacagttg cgggtagtga tgaaccatcg cagtacacga tcccgagggg
     6181 ctgacaatcg catcagcctt gctctcttca aagatctcga taaagcgacg ggcaaccggg
     6241 cgcgcctcgt cgagatagcc cgtgttgaaa gccggctgag cgcaacacgt ctgtcgcttg
     6301 tcaaactcaa cctcacatcc gacacgccgc aacacctcaa cactgctcac gccgacgctg
     6361 gtccacaact gatcgacgag acaagttaca aaaagcgata ccttcataca gtatttagcc
     6421 gcggatgaac gcggattttc gctgatctgc gttcatccgc gaagatccgc ggctaaaaca
     6481 acgactccct ttacataatc ggcggcgtgg atatctgtgg acaatgcctt ctcgacttct
     6541 tcgaacgatg tgtaacgatg cgtgatcagc ggtgcgatat cgatctgctt ctgttcgatc
     6601 aacttcaacg cgcgcgtgta aaccgacggc cggccatcac tctcaaaccc accggacgca
     6661 ccgacagaac tcaccagcac aggctccttg aaaaaaatgc tgctcaacac gctcaaatca
     6721 gttcccgcat gtccgtgccc ataacacaac acggtcgcct gtttgcggat caacccgggc
     6781 atcacccgaa acacatcacc cgatcccgaa gcttcgatca agtactctac acccttgccg
     6841 ccagtgtgct cgtggaccac ctcaaccaga tctgaagtta ccggatcgat cgcctcgtca
     6901 gcgcccaatt gcctggcgag ctgtcgcttg cgctcatttg gatcgctgac aaacaaccat
     6961 ccctcgtaaa ggagcacgtt tcgcaaatac tgtgtgaata ataatcccgc cggtccggcg
     7021 ccgcatatta acacgctgcg cacccggcgc tccgcgcttt cagcccgcac cgcatgccgc
     7081 gtgcgcgcct tcgccaccgt gtccgacgaa tggatgatgc agccgagtgg ctcgacgagc
     7141 gccgcgatcg atgcatcgag cggcatagtg atctttacag cattaacagc cggaacagca
     7201 ataaactcgg ccaacccgcc aggcaatccg gtaattccat gttcgcgata aaactcgcat
     7261 tgatgactat tctcagtgcc acaatagtca caaagacctt cgcgccgccg gctgacgcaa
     7321 ttcaagccct ggtccaccac cactcgatcg ccttcgctga gatcgcgaac cgcactgcca
     7381 acctgttcga cgacgcccgt gatctcatgc ccgagaatct gcggttgcaa agcaagcgga
     7441 atcgcctgcc catactcgtt cgtgttgtag tttgcgtgac cagcaaagat gtgcacgtcg
     7501 gttccgcaaa gaccgacagc cgcgacgcga atcagcacct cgtaaggcga gatcaccggt
     7561 cgtggaatgt cgcgcacctc gagacgattg acgtcgcaca acacactcgc cttcatcgtc
     7621 tggctttcca tctgcgttta tccgcgtaaa tccgcgtttc agatcaaatc gctggtgtcg
     7681 agtcgatcag cgtcacgaag cgccgcaaac cgatccatcg tttcacgtat gccggtttca
     7741 agcggcgtcg acggcaacgc accaatcgta ttcctgatcg ctgagtcgtc catggcggca
     7801 gctatcggaa tcggtggacc accaaaagtg atcaagtcac cacttacaga gttaatgaaa
     7861 tttgcgatgc gatcgacagt gaccgtttca ccgtgaagat tgaaaacgtg tgcgccttcg
     7921 ggtgcgttgt cggcacacgc gagaaatgct gctgcggtat cggcaacgta ttgaaagtct
     7981 gtcgcgccgc cgaagcgaat gtgaaacggg cggccgagca ctgccgattt catcgccttg
     8041 gttggatcgc ttgttagccc tgtatcacga ttcactccgt agacggttaa cg
//
LOCUS       DASSFQ010000183        13197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_84185, whole genome shotgun sequence.
ACCESSION   DASSFQ010000183 DASSFQ010000000
VERSION     DASSFQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84185"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3743)
                     /locus_tag="VFH96_06430"
     CDS             complement(<1..3743)
                     /locus_tag="VFH96_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973644.1"
                     /translation="MNSPFYRIIRSLILASSLLLFSFTSIVAQQQSTDGATPLGLSPG
                     APAGSYPLSDFENVNLYNGTLNFSLPLLKIGGRGEAGYSLMMRVDHKWLVQKEPYDGQ
                     PPVNIYTPMPGWWTDIGWAQIYSMGRVETRQGGSRDFVLGSGGCGYIHRLTLTRLTFI
                     APDGTEYELRDQATNGQPVLATCTAYNRGTVFVTADGSAATFISDTNISDYQYDNPAN
                     VPPSGYMMLKNGTRYRVDGGKVTWMRDRNGNKLTFNYDNFMRMTSVTDSLNRQVTINY
                     DAGSGAYQEITYKGFGGATRSLKVYFNYLSNVLRSDFTLLTTGQMFPELNGAGSGYHN
                     PQVVSAVELPNGKQYQFRYNSYAELTRVILPTGGAIEYDYAAGLTDSASGSGVVSGLA
                     QKEIYRRVIERRVYPDGGSGSGFASRMTYSRPETSTTNAGYVTTDQYNASGTLLTRAI
                     HYFYGSPRASLFQQPTEYAAWTDGREYKTEAFAANGTTLLRRVENTFQQRATVSWWTG
                     GAATAPPNDVRMSETVTTLADTNQISKQAFGYDDTVPFNNQNNVKEYDYGAGAVGPLV
                     RETRTTYLTSASYTGTSVHIRNLPTQVSIYDGGGVERARTTYEYDNYPLDSTHSPLTN
                     RPSISGFDATFTTGYPTRGNVTGTTRYLLSGGAVTGSLSSYSQYDIAGNVTKVIDGRG
                     NATELNYDDCFGAPNGEARTTSAPAELAGLSSYAFITSIKNPLNHWTYSQFDFYLGAP
                     VDGEDANGVVASGTYNDSLDRPTQIRRAAGTSITNQTTFSYDDTNRIITTSSDRDTNN
                     DNALVNSVVYDQMGRTKETRQYVGGTNYIAVQSEYDALGRLYKTSYPFRPWLSETPVW
                     TTTAFDTLSRATSVTTPDSAAVSSSYSGNSVTVTDQAGKARKSVTDALGRLSEVYEDP
                     AGVNYQTSYLYDSLDNLARVTQGSQQRFFMYDSLKRLIRADNPEQETLSALNLTDPAT
                     SHSNWTVKYEYDNNGNLTSKTDARGVVTENRYDALNRLDTVLYRINGQPDPNTGDIQY
                     VYDNATYGKGRLWLTYRWGAKPSHTTVGEYDAMGRVKQLWNDFSDGQGGWLPGYGISR
                     NYNLAGQVTSQTYPSGHTVNYSYDAAGRTSSVTGNLGDGVTRSYASSFVYNARSQVTQ
                     ELFGTQTPLYHKLQYNIRGQLWDVRVSTNNDINGSSNRGGLQYFYEGNLGYGTSGPDN
                     NGNVLFANIHSPEDEQMVHWATTRQGYTYDSLNRLKSVKEYFK"
     gene            complement(3805..4227)
                     /locus_tag="VFH96_06435"
     CDS             complement(3805..4227)
                     /locus_tag="VFH96_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973645.1"
                     /translation="MPVKLKYSLLLLLVIGLATSSALVRSYAGRNPTKLETSSVAKAS
                     IVSADDVPQVISLQVTPSGFEPRETIAPKGKFLILLQNRTGRRDLNFWLARENEGRVA
                     ESKSQNRDWKAQVQLAPGAYIIGETNHPEWQSIIRVTN"
     gene            complement(4341..6269)
                     /gene="selB"
                     /locus_tag="VFH96_06440"
     CDS             complement(4341..6269)
                     /gene="selB"
                     /locus_tag="VFH96_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HET6973646.1"
                     /translation="MSTHSLIVGTAGHIDHGKSALVRALTGVDPDRLPEEKRRGITID
                     LGFADLELDDLRIGFVDVPGHERFVKNMLAGAHGIDLLALVIAADEGVMPQTREHFDI
                     CRLLGVSNGLVVITKTDLVDEEMLVLVEDDAHELIAGSFLEDAPLVSVSSRTGAGMDQ
                     LKSQLVDLGKRVPPRSKDFTTRLPIDRAFSMKGFGAVVTGTLISGKITEGDELELLPS
                     KVNVRVRGLQVHNKSVHDAHAGQRTAVNLAGIDTAQIERGMVLAPVGRLRPTQIVDAW
                     IDVLPGASRAVRSRSRVRFHIGAAEVLGRVRVLGGSSVEAGSGGLVQLRLEAPVVAVH
                     GDRFILRSYSPAETIAGGVIVDPFATKHRGREMDHALELLRLLMRDERTSKFEGFVRA
                     AGVRGVRLADLAAATGWTNEVLANVAQEVQQRGSVIEVGGLYIARDSLERLSTALIAE
                     LERHHKREPLARGMLRETLREKVFTHSAPELFNGVLARLEAEGKVVSEKDIVRSSEHS
                     VGLSDKEVELSNRIEQIYLTAGVEAPSLDDAMAKANVPAAQRAQARKLLQLLLDDRKL
                     VRIQGEMFMHSKVVQDLKTKLHTYASQHEPDRLIDVPAFKDLAGVSRKYAIPLLEYFD
                     REQITRRAGDKRLILKPR"
     gene            complement(6284..7417)
                     /gene="bshA"
                     /locus_tag="VFH96_06445"
     CDS             complement(6284..7417)
                     /gene="bshA"
                     /locus_tag="VFH96_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HET6973647.1"
                     /translation="MNIGITVYPTYGGSGIVGSELGKELAQRGHTVHFISSALPTRLT
                     ELNERVRFHEVEMMSYPLFEHQPYTLALATKMATVAEEENLDLLHVHYAIPHSISAIL
                     ARESLKPRRRLPVVTTLHGTDITLVGADRSYLPITRYGIVQSDGVTAISNYLKDATHE
                     IFHFDDIKVIPNFVCQYDYKRLEVDSLRETLAPQNEPLLVHVSNFRPVKRPVDCVEIL
                     ARVLKKGVKTRLIMVGDGSERPNAEHRARCLGIYDQCSFVGKQPRIVDYLSASDVLLL
                     PSDQESFGLAALEAMACEVPVIASRVGGLPEVVTDGETGFLSPVGDVDKMAADAARLL
                     SDGKFRREMGHRARESAISRYRTDVVIPQYIEFYEQVIAKQSD"
     gene            7700..8278
                     /locus_tag="VFH96_06450"
     CDS             7700..8278
                     /locus_tag="VFH96_06450"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET6973648.1"
                     /translation="MKGYNVGMPKISDIEETPNPNAVKFILREAVSNGVARQYGSAEL
                     AENDQLAKSLFDVGHVVSVFYMDRMITVEKDDEGDWDELLPALAVPIRAADAVNGAAA
                     AAAAVGGPIAAVTSDDPRLLRINEILDEKVRPALMGDGGYLEIIGLSEHTLSIRYQGA
                     CGSCPSSLTGTLMAIESMLKQEVDPELEVIAV"
     gene            8419..10296
                     /locus_tag="VFH96_06455"
     CDS             8419..10296
                     /locus_tag="VFH96_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973649.1"
                     /translation="MRLPYAAIACLCLALFVPVTFAQPKKAKPTTTPAATEDADAEAA
                     QQRTVAISLVSTLADEARSFKDQTRRARVQARAADVLWDTDPERARELFRRAWEAAEI
                     VDAESARKRTEEMKRLETAGEPVVVRGGPDIRSEVLRLVAKHDRKLGEEFLKALDEAN
                     EKQRSEAAADQRRNDPGAQLGISQRLQLARRLVEDGEVERAIEFATPAFTAVSPDTIF
                     FLSALREKNAQVADSAYAALMARAARDPNSDANTVSGLSSYLFTPFLYVTFEPSGGAN
                     QSRQRGPTPAPEVDQTLRNNFLKVAYQILMQPLAAPDQDRTKSGQTGKYLMIKRLLPL
                     FEQYAADLAPGLRTQMTALASYVPSTFQQGDNRAVTVGIRPESEEGDPAQRMQERLDR
                     AKTSEERDAIYADYAVAFSDKGDPRGSDLVDKIENTELRKSVKAYTDFQLVQLAVRKK
                     DVTEITRLVKSGDLTSIQKVWALTSAAKFVMANERSRATDLLDEALAESRRISASDPD
                     RGRALTAVAVGLAEIDRVRAWEVLNEVVKAANGAETYTGEDSRIASVLRTKMMVVVSQ
                     STAEDFDLLAVFRHLARADLLRAIQLAKTFTGEAPRAVATLAIARTVLEKRRDIAAAR
                     N"
     gene            10385..12265
                     /locus_tag="VFH96_06460"
     CDS             10385..12265
                     /locus_tag="VFH96_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973650.1"
                     /translation="MKRINLTLCLLVVVTVTQAQTQTPDTKPAPPAKTVAPKSAAITE
                     EELAQRERRAKARALLVALSSDARTFHDEMLRARSLARIADALWQVDAEQGRLLFRKA
                     WDAAEVADIESDKKLQEEIRQQQLRTGSKGYVANSPPNIRREVLRLAAKHDRALGEEF
                     LEKLRVQKLEAATSATAKPSPNNLSEAMNQRLSVARELLQNGELERAVQFADPALALV
                     TVESMNFLSFVREKNANLADARYAALLASSNNNPQADANTVSLLSSYIFTPHLHMVFN
                     GTSTSTSQSASTITPAAVSPELKNAFFQTAATILLKPLPPPGQPDTSSAGLDGKYLVI
                     KRLLPFFEQSAPPAMVESLRGHMSALSTMISDGARRYDQDSLSEGLRAEKPAVDREQS
                     LLDKVERAKTSDERDSLYVQLAQFAAHQGDMRARDYVGKVENTELRKQAQAYIDGSLA
                     VHFVEKKQPEQALELAHKAELTQMIKTWVLAQSAKLLFKTDKDKAQEVLDEAATEARR
                     LDVSDPNLPRALVGVANALKLIDPPRVWDATFDAVKAANSAEGFTGEDGEIVLMFQSK
                     NGSSVRTDDVPDFDLEGIFKDLALQDYDRAVELARGFQGEGPRAVATIAIARAILEPK
                     RR"
     gene            complement(12294..13040)
                     /gene="bshB1"
                     /locus_tag="VFH96_06465"
     CDS             complement(12294..13040)
                     /gene="bshB1"
                     /locus_tag="VFH96_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HET6973651.1"
                     /translation="MSLSEIQLDILAVFSHPDDAELSVAGTLLKLKSQGYRTGVCDMT
                     RGEMGTRGTPEIRAKEALDAARTMKLDVRLNLEQPDGHVWPTEVARTALVRVLRTHQP
                     KVLITTHEDDPHPDHANTSIIVRQAARLATMKRYDEEPGLAPVPMPAIMHSLFSRRIV
                     PSFVVDVSDFVDEKMNAIRAYGSQFYDAASKEPATRISQRGFLSEIEYRMRYFGSLIG
                     VEAGEPFYVREALNVDDPLALLTRPMNIYS"
BASE COUNT         2990 a         3669 c         3686 g         2852 t
ORIGIN      
        1 tttttgaaat attcttttac cgacttcagg cggttcaacg aatcgtaggt gtaaccttgc
       61 cgggtagtgg cccaatgaac catctgttcg tcctcaggcg agtggatgtt cgcaaagagc
      121 acattcccat tgttgtccgg accgctggtc ccgtagccta aattgccttc gtaaaaatat
      181 tgtaagccgc cacgattcga gctgccgtta atatcgttat tagtcgagac gcgcacatcc
      241 cataactgac cgcggatgtt gtattgcagc ttgtgataga gcggtgtttg cgtcccgaat
      301 agttcctgcg tcacttgact gcgcgcgttg taaacgaacg acgaggcata actgcgcgtg
      361 acgccatcac ccaggttgcc ggtaacactg ctggtgcgac cggctgcatc gtaagaatag
      421 ttgaccgtgt gacccgaggg ataagtctga gaggtaactt gccccgcaag attgtagtta
      481 cgactgattc cgtaacctgg caaccaccca ccctgtccgt cactgaaatc gttccacaac
      541 tgcttcacgc gacccatggc atcgtactca cccacggttg tgtgcgacgg tttcgcaccc
      601 cagcgataag tcagccataa gcgacctttg ccataggtgg cgttgtcgta cacatactgg
      661 atatctccgg tgtttggatc gggctgcccg ttgatgcgat acaacacggt atctaaccgg
      721 ttaagtgcgt cgtagcgatt ttcagtgacc actccacgcg catcagtttt ggaggtgaga
      781 tttccattat tgtcgtactc gtatttgacc gtccagttac tgtgactcgt agccggatca
      841 gtgagattca gcgcgctcag agtctcttgt tccggattgt cagcacggat cagtcgcttc
      901 aacgagtcgt acatgaagaa gcgctgctgg ctgccctgcg tcactctcgc cagattatct
      961 aaggagtcgt acaggtagct ggtctgataa ttcactcccg cgggatcttc gtaaacttcg
     1021 ctcaaacgtc cgagcgcatc agtcacgctc ttacgcgcct tacctgcctg atcggtgacc
     1081 gtgacgctgt tgccgctata gctgcttgaa accgccgcgc tgtcgggcgt cgtcaccgag
     1141 gtggcgcggc tcagcgtgtc gaatgcagtc gtggtccaga cgggcgtctc gctcagccac
     1201 ggtctgaagg gataggaagt tttgtacaaa cggcctaacg catcgtattc cgactgaacc
     1261 gcaatgtagt tggtcccgcc cacgtactgc cgtgtctctt tggtgcggcc catctggtcg
     1321 tagacgaccg agttaaccag tgcattatcg ttattagtgt cgcgatcgct cgaggtggtg
     1381 atgattcgat tggtatcgtc gtaactgaag gtggtctggt tggtaatact ggttccggcc
     1441 gcgcgtctga tctgcgtggg ccgatcgaga ctatcgttgt aagtgccgct ggcgaccacg
     1501 ccgttggcat cttcgccatc aactggcgca cccaggtaaa agtcaaactg gctataggtc
     1561 cagtgattca gcgggttctt gatggaagtg ataaatgcat aactggaaag acccgcgagc
     1621 tcggcgggcg ctgaagtcgt ccgcgcttcg ccattcgggg ctccaaagca atcgtcgtaa
     1681 ttaagctcgg tagcgttacc gcgcccatcg atgactttag ttacattgcc ggcgatgtcg
     1741 tattgcgaat acgacgagag cgagccggtc acggcgccac ctgacaacaa gtaccgcgtc
     1801 gtgccggtca cgttcccgcg cgtcgggtag ccggtagtga aggtcgcatc aaaaccgctg
     1861 atgctggggc gattcgtcaa aggcgagtgg gttgaatcca acggatagtt gtcatattca
     1921 taggtggtgc gagcccgctc aactccgcct ccatcgtaga tcgagacctg cgtcggtaga
     1981 tttcggatat ggacgctcgt tccggtataa cttgcactcg tcagataagt ggtgcgtgtt
     2041 tcgcgtacca gcggtccaac tgcacccgcg ccgtagtcgt attccttcac gttgttttgg
     2101 ttgttgaaag gaacggtatc gtcatagcca aacgcctgct tcgaaatctg attagtatcg
     2161 gcgagggttg tgaccgtctc gctcatacga acatcgttgg gcggcgcagt cgcagcacct
     2221 ccagtccacc agctcaccgt ggcgcgttgc tggaaagtgt tttccacccg gcgtagcagc
     2281 gtggttccat tagctgcaaa cgcctcggtc ttgtactcac gaccatcagt ccacgcggca
     2341 tattcagtcg gctgctggaa gagtgaagcg cggggactgc cgtagaagta atggattgcg
     2401 cgcgttaata acgtgccact ggcgttatat tgatcggtgg tcacatagcc cgcgttggtg
     2461 gtgctggtct cgggccggct gtaggtcatg cggctggcga aaccactgcc actgccgcca
     2521 tccggataga cgcgccgctc aatgacgcga cgatagattt ctttctgggc caggccactg
     2581 accacgccgc tgccgctggc ggaatcagtt aagccggcag cgtagtcata ttcgatagct
     2641 ccgccggtcg gcagtattac gcgcgttaac tcggcgtagc tgttatagcg aaactggtat
     2701 tgcttgccgt taggcagctc aaccgcactc accacttgag ggttgtgata accgctgccc
     2761 gcgccattca attccggaaa catctggccc gtggtcagca gcgtgaagtc actgcgcaac
     2821 acgtttgaga ggtaattaaa ataaactttg agactgcgcg tagcaccgcc aaagcctttg
     2881 taggtgatct cctgataggc accactgccc gcatcgtagt taatcgtcac ctgccggttc
     2941 aaagaatcgg ttacggacgt cattcgcata aaattatcgt aattgaaggt gagcttattg
     3001 ccgttacgat cgcgcatcca cgtcactttg ccaccgtcca cacgatagcg cgtgccgttc
     3061 ttcagcatca tgtagcccga cggtggcacg ttggcgggat tgtcgtattg gtaatcagaa
     3121 atatttgtat ctgaaatgaa agtcgcggca cttccgtcgg cggtcacaaa aacagtgccg
     3181 cggttatagg cggtacaggt ggccagcacc ggttgaccgt tagttgcttg atcgcgcaac
     3241 tcgtactcgg tgccgtcagg cgcaatgaac gtcaaccgcg ttagcgtcag ccggtgaatg
     3301 tagccacacc cgccggagcc cagcacgaaa tcacggctgc cgccctgacg cgtctccacc
     3361 cggcccatgc tgtaaatctg agcccagccg atgtcagtcc accaacctgg catcggtgta
     3421 tagatgttga caggcggttg gccatcgtag ggttccttct gcaccagcca cttatgatcg
     3481 acccgcatca ttagcgaata cccggcctca cctcgtcccc cgattttcag caacggcaga
     3541 gagaaattca gagtaccgtt atagaggttt acattttcga aatcgctcag tgggtaggag
     3601 ccggcaggag ctccgggtga aagcccgagc ggagtcgcgc cgtccgtaga ctgttgctgc
     3661 gcaacgatac tcgtgaatga aaaaagcagc agagatgatg caagaatcaa agagcgaatg
     3721 atgcggtaga agggcgaatt catatcggac ctcgatcaat aaggaatgag agtgtttgtt
     3781 atggaaactg cgaccgctgc gcgtttagtt cgtcacgcga ataatcgact gccactcagg
     3841 atgattggtt tcaccaatga tgtaggcgcc gggagcgagt tggacctgcg ctttccaatc
     3901 gcggttttga gattttgatt cagccactcg tccttcgttt tcacgcgcca gccagaagtt
     3961 gagatcgcgc cgaccagtgc gattttgcaa aaggatcaga aacttcccct tcggcgcaat
     4021 tgtctcgcgc ggttcgaaac cggacggtgt gacttgcaaa gagatcacct gcggcacgtc
     4081 gtccgcggaa acgatcgagg cttttgctac ggaactggtc tccagcttcg ttggattccg
     4141 gcccgcgtac gatcgcacca acgcagaaga cgtcgctaga ccgatgacta gaagaagtag
     4201 taaggaatac ttaagcttca cgggcatttg cctcctggga taacactgaa tacaaggtaa
     4261 agagggggag aaagtgggcg ggactttact cctgtgttgg accactgtca atacgaaaat
     4321 ttagaccacc tggcattttt ttagcgaggc ttgaggatga ggcgcttgtc gccggcgcga
     4381 cgggtgattt gttcgcggtc gaagtattcg agaagaggaa tggcgtattt gcgagagacg
     4441 cccgcgagat ctttgaacgc agggacgtcg atcagacgat cgggttcgtg ttgtgaggcg
     4501 taggtgtgca gtttggtttt tagatcctgg accactttcg agtgcatgaa catttcgccc
     4561 tggatgcgga cgagtttgcg atcgtcgagc aacagttgca ggagcttgcg agcctgggcg
     4621 cgttgggctg caggtacgtt tgcttttgcc atcgcgtcgt caagtgatgg agcttcgact
     4681 ccggctgtga ggtagatctg ttcgatgcga ttgctgagct cgacctcttt atctgacaag
     4741 ccgacactgt gttcactcga acgcacgatg tctttctcgg aaactacttt gccttcagct
     4801 tccagccgcg cgagcacgcc gttgaataac tcaggcgcag aatgcgtgaa gactttctcg
     4861 cgcagtgtct cgcgtaacat gccgcgcgcg agcggttcgc gcttgtgatg cctctccaac
     4921 tccgcgatca acgcggtgct gagacgttct aaactgtcgc gcgcgatata cagcccgcca
     4981 acctcgatca cggaccctcg ctgttgaact tcctgcgcca cattcgcaag tacttcgttg
     5041 gtccaaccag tcgctgccgc aaggtccgcg agccgcacgc cacgcactcc cgcagcccgt
     5101 acgaaacctt caaacttcga cgtccgctcg tcacgcatca ataagcgtaa cagctccagc
     5161 gcgtgatcca tctcacggcc gcgatgtttc gtcgcaaacg gatcgacgat cactccgcct
     5221 gcgatcgtct cggcgggcga atagctccgc aaaataaacc gatctccgtg cactgcaacc
     5281 accggcgcct ccagccgcaa ctgcacgaga ccgccactgc cagcttctac cgacgaaccg
     5341 cccaacacgc gcacccgtcc cagcacttca gccgcaccaa tgtgaaaccg aactcgcgaa
     5401 cgcgagcgca ccgcacgcga cgcaccaggc aacacatcaa tccacgcatc gacgatctgc
     5461 gtcggacgca aacgtccgac aggcgcgagc accatgccgc gctcgatctg cgccgtatca
     5521 atgccggcga gattcactgc cgtacgctgt cctgcatgtg cgtcgtgaac cgacttgtta
     5581 tgtacctgca acccacgcac gcgcacattg actttcgacg gcaacaactc gagctcgtcg
     5641 ccttccgtaa tcttcccgga aatcagcgta ccggtaacga cggctccaaa tcctttcatc
     5701 gaaaacgcac gatcgatcgg aagacgcgtc gtgaaatctt tcgaacgcgg cggcacacgt
     5761 ttgcccagat cgacaagctg cgacttcaac tggtccatcc ctgcgcctgt gcgcgagctc
     5821 accgaaacaa gcggcgcatc ttcgagaaac gatccagcga tcagctcgtg cgcgtcgtcc
     5881 tcaaccagca caagcatttc ttcatcaacc agatctgtct ttgtgatcac aacgagacca
     5941 ttgctgacgc cgagcaagcg gcaaatatcg aaatgctcgc gcgtctgcgg catcacgcct
     6001 tcatcagccg cgatcacgag ggccagcaga tcgatgccgt gcgctcccgc cagcatgttt
     6061 ttgacgaaac gctcgtgacc gggaacgtcg acgaaaccga tgcgcagatc atccagctcg
     6121 agatcggcaa agccgagatc gatcgtgata ccgcgacgtt tttcttcagg aaggcgatcg
     6181 ggatcgacgc cggtcaaagc gcgcacgaga gcgctcttgc catgatcgat gtgaccggca
     6241 gtgccgacga tcagtgaatg cgtgctcaca gattaaacgg atttcagtcg gattgttttg
     6301 cgattacctg ctcgtaaaat tctatgtact ggggaataac gacgtcggtg cggtagcggc
     6361 tgatggcgga ttctctggcg cgatgcccca tctcgcgacg aaatttcccg tcagaaagca
     6421 gacgcgcagc gtccgcagcc atcttgtcga cgtcgcccac cggactcaga aacccggtct
     6481 caccatcagt cacgacttcc ggaagaccac cgacacgtga cgcgatcacc ggaacttcgc
     6541 acgccatcgc ttccagcgcc gccagtccaa acgattcctg gtctgacggc agcaacaaca
     6601 catcagacgc tgaaagatag tcgacgatgc gcggctgttt gccgacgaat gaacactgat
     6661 cgtaaatccc gagacagcgc gcgcgatgct ccgcgttcgg gcgttcactg ccatcgccga
     6721 ccatgatcaa acgcgtcttc acgcctttct tcagaacgcg cgcaaggatc tcgacgcagt
     6781 cgaccggtcg cttaaccgga cggaaattcg agacgtgaac cagcaacggc tcgttttgcg
     6841 gcgcgagcgt ttcgcgcagg ctgtcgactt cgagacgctt gtaatcgtac tggcaaacaa
     6901 aattcggaat gaccttgatg tcgtcgaagt gaaagatctc atgcgttgcg tcttttaagt
     6961 aattggagat cgccgtgaca ccgtccgact gcacgattcc ataacgcgtg attggcaaat
     7021 acgatctgtc agcaccgacg agcgtgatgt ctgttccgtg caaggtggtg acaacaggca
     7081 gacgtcgtcg cggcttgagc gactcgcgcg cgagaatcgc actgatcgag tgtggaatcg
     7141 cgtagtgaac gtgcagcaga tcgagatttt cttcttcggc gacggtcgcc atcttcgtcg
     7201 cgagcgcgag tgtgtaaggc tggtgctcaa acaacgggta cgacatcatt tccacttcgt
     7261 gaaaacgcac ccgctcgttg agctcagtca atcgtgtcgg cagcgccgaa gaaataaagt
     7321 gaaccgtgtg accgcgttga gcgagttctt tgccgagctc agaaccaacg atgccgctgc
     7381 cgccgtaggt aggatatacc gtgatgccga tgttcattgc gctctcaaat tagcaagcac
     7441 aaagcacaag ttcaaccgca aaaaacgtga ggcaggcgac cctcgctatc gcctgcctct
     7501 atctcaactt gactcacctt ctgagagggg gggcaagtga ggagcggagt atattcctgc
     7561 ttttaaaaat tagcaatgat tcttgcaacg acgtgtagtg gttcactttc gctgcaaagt
     7621 gtccgataaa cgttagtttg tcgcgacatt ccgacaagct aaagcttatc ggacatcatg
     7681 tgcgctcggt tctgtcagtg tgaagggcta taatgtcggc atgccaaaga ttagtgatat
     7741 tgaagaaaca ccgaacccga atgccgtgaa attcatcctg cgcgaggccg tcagtaacgg
     7801 cgtcgcacgg caatatggtt cagcagaact ggctgaaaac gatcagttag cgaagtcgtt
     7861 gtttgatgtg ggccacgtag tttcggtttt ttacatggac cggatgatta cggtcgagaa
     7921 agacgacgag ggcgactggg atgagctact gcccgcgctc gccgttccga ttagggcggc
     7981 ggatgcagtg aatggtgcgg cagcagcggc cgcggcagtc ggtggaccaa tcgcggcagt
     8041 gacgagcgac gatcctcgcc tgctgagaat caacgaaata ttggacgaaa aagtacgacc
     8101 ggcgttgatg ggtgacggcg gttacctcga aatcattgga ttgagtgaac acacgctgag
     8161 cattcgttac cagggtgcgt gtggaagctg tcccagttcc ctcaccggca cactgatggc
     8221 tatcgagagc atgttgaagc aggaagtcga tcccgagtta gaggtcattg ccgtctgatt
     8281 cacagattcc gcaggttgca cagaaaaaat ccgtaaaaaa atctgtgtaa tctgtggatg
     8341 ttttggaacc tgcccgccgc gcgcgcgtgg aaccgttcgg ttacttccat ccgcatttca
     8401 gacctacagg agtgttctat gcgcctaccc tacgccgcaa tcgcgtgtct ctgccttgct
     8461 ctgttcgtcc cagtcacttt cgcccagcct aaaaaagcca agccaaccac aactccggcc
     8521 gctaccgaag acgccgacgc cgaggccgcg cagcaacgca ctgtcgcgat ttcgctcgtc
     8581 agcacactcg ctgacgaagc ccgcagcttc aaggatcaaa cgcgacgcgc gcgcgtgcag
     8641 gcacgagctg cagacgttct gtgggacacc gatcccgagc gtgcgcgaga acttttccgc
     8701 cgcgcgtggg aagcagctga gatcgtcgac gccgaatccg ccaggaaacg caccgaagaa
     8761 atgaagcgac tggaaactgc aggcgagcca gtcgtggtcc gcggcggtcc tgatattcgc
     8821 agcgaagtcc tgcgactcgt cgccaagcac gatcgcaaac tcggcgaaga gtttctcaaa
     8881 gcactcgacg aagcaaacga gaaacaacgc agtgaagctg ccgcagatca acgtcgtaac
     8941 gatccgggcg cgcaactcgg catatcgcag cgtttgcaac tggcacggcg cctcgtcgaa
     9001 gacggtgaag tcgaacgcgc gatcgaattc gccactcccg cgttcaccgc agttagtccc
     9061 gacacgatct tctttctctc agcactgcgc gagaagaatg cgcaagtcgc agattcagct
     9121 tacgcagcac tgatggctcg tgcggcgcgc gatccaaact ctgacgccaa caccgtttcc
     9181 ggtctgtcgt catacttgtt cacaccattt ctttacgtga cattcgagcc cagcggtggc
     9241 gcgaatcaaa gccggcaaag aggtccaacg cctgcacccg aagtcgatca aacgctgcga
     9301 aacaactttc tcaaagtcgc ttaccagatc ctgatgcaac cactcgccgc accggatcag
     9361 gatcgcacca agtcaggtca aaccggaaag tacctgatga tcaaacgact gctgccgttg
     9421 ttcgaacagt atgctgcgga tctcgcgccc ggcttgcgca cacagatgac cgcgctcgct
     9481 tcgtatgttc cgagcacatt tcagcaaggt gacaatcgtg ctgtcactgt cggcattcga
     9541 ccagagtcgg aagaaggcga tcctgctcaa cgcatgcagg agcgtctcga tcgcgcgaag
     9601 acgtcggaag agcgcgatgc gatttatgcc gactatgcag tcgcgttctc tgacaagggc
     9661 gatccgcgcg gaagtgatct cgtcgataag atcgagaaca cggagctgcg caagagcgtc
     9721 aaggcttata cagattttca gctcgtgcaa ctcgcagtgc gaaagaaaga cgtcaccgag
     9781 ataacccggc tcgttaaaag tggcgatctc acgagtattc aaaaggtgtg ggcgttgacc
     9841 agtgcggcga agttcgtcat ggcaaacgaa cgctcacgcg caaccgatct gctcgacgag
     9901 gcactcgcgg aaagtcgtcg catcagtgcg agcgatccgg atcgtggacg agcgttgacc
     9961 gcagttgctg tcggacttgc ggagatcgat cgtgttcgtg cgtgggaagt tctcaatgaa
    10021 gtggtgaaag cggcgaacgg cgccgagact tacactggtg aagacagccg cattgcttca
    10081 gtcctgcgca cgaagatgat ggtagtggtg tcgcaaagca cggctgaaga ttttgactta
    10141 ctggcggttt tcaggcatct tgcgcgtgcc gatctacttc gagcgattca gcttgccaaa
    10201 acattcacgg gagaagcgcc tcgggctgtt gcaacactcg cgattgcacg cacagtgctc
    10261 gagaagagaa gagatatagc agctgctagg aattaattgt aggggcggcc ctccgtggcc
    10321 gcccgttcgc ggattcacct gacgggtggc cacggagggc cacccctaca actccttcca
    10381 caatatgaaa agaatcaatc tcaccctatg tctattggta gttgtcacgg ttacgcaggc
    10441 acagacgcag acacccgata ccaagccggc gccgcctgcg aagacggtag cgccgaaatc
    10501 tgctgcgata acagaagaag agcttgcgca aagagagcgt cgcgcgaagg cacgcgcgct
    10561 actcgtcgcg ctttcgagtg atgcgcgcac cttccacgac gagatgttgc gcgcacgatc
    10621 gctcgcacgc atcgctgacg cactctggca agtcgatgcc gaacaaggtc gattgttgtt
    10681 tcgcaaagcg tgggacgctg ctgaagtcgc tgacatcgag agcgacaaaa aacttcagga
    10741 agagattcgc caacaacagc ttcgcaccgg tagtaaaggt tacgtagcca atagtccacc
    10801 caacatacgt cgtgaagtgc tgcggctggc agcgaaacac gatcgcgcgc ttggagaaga
    10861 gtttctcgag aagctgcgag ttcaaaaact cgaagcggca acctcagcca ccgcaaaacc
    10921 aagcccgaac aatctcagcg aggcgatgaa ccagcggttg tcggtggcga gagaattact
    10981 tcagaacgga gaattggagc gcgccgtgca attcgctgat ccggccctgg ctctcgtcac
    11041 ggtggaaagc atgaacttcc tcagttttgt gcgcgagaaa aacgcgaatc tcgcagacgc
    11101 ccgttacgct gcactgctgg ccagctcaaa caacaatccg caagccgatg ccaatacagt
    11161 atcgttgctc tcgtcgtata tctttactcc tcaccttcac atggtcttca atggcaccag
    11221 cacatcgacc tcgcaatcag cttcgacgat cacgccggct gcagtctcac ctgaattgaa
    11281 gaacgcattt tttcagacag cggcaacgat cctcttaaaa ccgttgccac cgccgggaca
    11341 accagacaca agctcggcag gtcttgacgg aaagtatctc gttataaaac gtctgttacc
    11401 gttcttcgaa caatcagcgc cgccggcgat ggtcgagtcg cttcgcggtc acatgagcgc
    11461 gttgagtacg atgatttctg acggtgctcg ccgttacgat caggattcgt tgagcgaggg
    11521 gctacgggcc gagaaacctg ccgtcgaccg tgagcagtct ttgctcgata aagtcgagcg
    11581 cgcgaagaca tctgacgaac gtgattcgtt gtatgtgcag ctcgcccaat tcgctgctca
    11641 ccagggcgat atgcgggctc gcgattatgt cggcaaagtg gaaaatacag agttgcgcaa
    11701 acaggcgcag gcttacatcg atgggtcgct cgccgttcat tttgtggaga agaagcagcc
    11761 ggaacaggct ttggagctgg cccacaaagc cgagttaact cagatgatca agacgtgggt
    11821 gctcgcgcag agtgccaaat tgctgttcaa gactgacaaa gacaaagcgc aggaagtttt
    11881 ggatgaggct gcgactgaag cgcgtcgact cgatgtgtct gatcccaatc tgccgcgcgc
    11941 gttggtggga gtggcaaacg cgctcaagct tatcgatcct ccgcgcgtgt gggatgcgac
    12001 attcgacgcc gtgaaagcag cgaactccgc ggaagggttt acaggtgaag atggcgagat
    12061 cgtgttgatg tttcagagca aaaacgggtc gtcagtgcgt accgatgacg tgcccgattt
    12121 cgatctcgag gggatcttca aagatttggc gctgcaggat tacgatcggg ctgtcgagct
    12181 ggcgcgggga tttcagggtg aaggcccacg cgccgtggcg accattgcga tcgcgcgggc
    12241 aatactcgaa ccgaaacgca gataaacgcg gattgaaaac gcggatcggg ttgttaagag
    12301 taaatgttca tggggcgcgt gaggagtgcg agggggtcgt cgacgtttag cgcttcgcgc
    12361 acgtaaaaag gttcgccggc ttcgacgccg atcagcgatc cgaagtatct catccgatat
    12421 tcaatctcac tcaaaaatcc gcgttggctg atgcgcgtcg ccggctcttt cgacgctgcg
    12481 tcgtaaaact gtgacccgta ggcgcggatt gcattcattt tttcatcaac gaaatcagag
    12541 acgtcaacta caaatgacgg cacgattcgt cgcgagaaaa gcgagtgcat gatggcaggc
    12601 atcggcaccg gcgcgagtcc cggctcctcg tcgtagcgct tcatcgtcgc gagtcgcgct
    12661 gcctgtctca cgatgatcga tgtgttcgcg tgatcgggat gcggatcgtc ttcgtgagtc
    12721 gtgatgagaa ctttcggctg gtgcgtgcgc agaacgcgaa caagcgcagt gcgcgccact
    12781 tcggtcggcc aaacgtgccc atccggctgc tcgagattca agcgcacgtc gagtttcatg
    12841 gtgcgcgccg cgtcgagtgc ttctttggcg cgaatttcgg gagtgccgcg cgttcccatc
    12901 tcgccgcgcg tcatgtcgca aacacccgtg cgatagccct gtgacttcaa tttcagcagt
    12961 gttcccgcaa ccgacaactc cgcgtcatca ggatgtgaga aaaccgcgag aatatcgagt
    13021 tggatctcgg acaaactcat ggacgaagtt tagcttaaaa tgacgatgtc cgacatgctt
    13081 tagcttgtcg gattgcgcga caagctaaag catgtcgggc attttcagat tggagatatg
    13141 atggtactcg tactcttgct ctctctactc atgggccaca acgatactcc gcttctc
//
LOCUS       DASSFQ010000184         4236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1711215, whole genome shotgun sequence.
ACCESSION   DASSFQ010000184 DASSFQ010000000
VERSION     DASSFQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711215"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..789)
                     /locus_tag="VFH96_06470"
     CDS             complement(46..789)
                     /locus_tag="VFH96_06470"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET6973652.1"
                     /translation="MTFLKPKVLRFESLPSTNTELARRASEGAAEGLAIVAEEQTAGR
                     GRLQRAWSSPKGAGLYFSILLRPTIPQNYWPLITFMAALAVGDALREAAGVETDIKWP
                     NDLLSGERKICGILAEAIDTPLGRAVILGIGINLTENAFPPELANVATSVSEAMGSAP
                     DREQILAALLDALSRWYSRFDEPAQIVDAWSNRSSFAIGKLVQVSNGDDVWQGTTCGV
                     EPDGALRLRTSSGEIKLVRAGDVYSVRAS"
     gene            790..1419
                     /locus_tag="VFH96_06475"
     CDS             790..1419
                     /locus_tag="VFH96_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973653.1"
                     /translation="MSDYTEPSAVAPGMRSSLRYLEIPVVMIALLAGAAFLTKMCYLS
                     LAFNTIFVLIFLTLFYSYVRIRLNIQIPLTLLALVFLALQVDALGNFFRMYGRQFGPM
                     QYDEFAHTMVQVLVSPIIVWLTGRVLQHFGYRLPLKLTAFFAATIVFSLSAIYEIIEL
                     WDEIYFGGHRIWGPYDTATDLQWDLCGIIVGTLFSCIMLRDTRDQLVSH"
     gene            1397..2056
                     /gene="modB"
                     /locus_tag="VFH96_06480"
     CDS             1397..2056
                     /gene="modB"
                     /locus_tag="VFH96_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HET6973654.1"
                     /translation="MINWFPIRLSFVVATAATLIALIAGSGLAWLLARKRFPGRNLVD
                     ALVTLPLVLPPTVLGYYLLVLLGTRSAIGGFLYRTLGIRLTFTVTAAIIAASIHALPL
                     VTKSLRAAFEGVSTELEAAARTLGLNNRRIFFRVTLPLAAKGVLAATALAFARALGDF
                     GVTIMIAGNIPGKTQTASVAIYDAVQAGRDNEAFTLAVTVSVIAGFMLYVINRFGRVR
                     W"
     gene            complement(2117..3352)
                     /locus_tag="VFH96_06485"
     CDS             complement(2117..3352)
                     /locus_tag="VFH96_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HET6973655.1"
                     /translation="MNRRFFLKSGSVALASVGMSLSAPSFLERIALGNTSGKRKTLIA
                     IFQRGAVDGLNVVVPFGESNYYEQRPSIAIPKSEAINLDGFFGLHPSLSAFKPLWDSK
                     RLAIIHASGSPDNTRSHFDAQDYMESATPGVKSTSDGWLNRYLRSKQDSDQSLFRAVS
                     MTKTMPRVLQGRAPAVAMSNLSDFAIRAGKSSENLQGGFEAIYARETKDSLAGMGRET
                     FEAVNYLKKANPAQYQPENGAQYPRNPFGTSLLQIAQLIKAGVGLEVAFTDIGGWDTH
                     VNQGNSRGQLANLLQQFSSGIAALYQDLGQRMDDVVVLTMSEFGRTVRENGNRGTDHG
                     HANAMFVLGNSVKGGKVYGQWPGLKSDQLYEGRDLALTTDFRDVFGEIATRHLGSTDV
                     KSIFPGYSSSASKFKGFLS"
     gene            complement(3355..>4236)
                     /locus_tag="VFH96_06490"
     CDS             complement(3355..>4236)
                     /locus_tag="VFH96_06490"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HET6973656.1"
                     /translation="RELMELHTLGVDGGYTQKDVQEVARCFTGWTIFQPRGGGAAVNA
                     MMGEAGRRNAGTFFFNARVHDDGEKTVLGHKIPAGGGIKDGLTVLDILAHHPSTAKFI
                     ATKLVRHFVSDMPPQSLVDRVAATFTKSDGDIRETLKAIFFSKEFNSTEAYRVKIKRP
                     FELVISAIRTLGADTNGGPGTHRWIERMGEPLYGFQTPNGYSDAAESWVNTGGLLERM
                     NFGLALANNRVQGTTVNLAKTNGEQAKVMDEYLKTLLAGDISAATREALLKQLEPSDT
                     ATKVVGLILGTPEFQRQ"
BASE COUNT          869 a         1259 c         1134 g          974 t
ORIGIN      
        1 caatcgtttg acaagatttc aggattttca agatttacaa gtcatttaag aagctctaac
       61 gctgtaaacg tctccggctc tcactaattt gatttcaccg ctcgacgttc gcagtcgtag
      121 cgcgccgtcg ggctcgacgc cgcaagtagt tccctgccaa acatcgtcac cgttactcac
      181 ctggaccagt tttccgattg caaaagaaga acgattggac catgcatcaa caatctgtgc
      241 aggttcgtcg aagcgcgaat accagcgcga cagcgcgtcc aacagtgccg ccaggatctg
      301 ctcgcggtca ggcgcggaac ccattgcctc cgacaccgaa gtcgccacat ttgcaagctc
      361 cggcggaaag gcattttccg taaggttaat cccgatcccg aggatcacgg cacgtcccaa
      421 aggagtgtcg attgcctcag ccaggatgcc gcagattttt ctttcacctg aaagaagatc
      481 gttgggccac ttgatatccg tttccacacc agccgcttca cgcaaggcgt cgccaaccgc
      541 gagcgctgcc ataaacgtga tcaaaggcca gtaattctga gggattgtcg ggcgcagcag
      601 gatgctgaag tacagccccg cacccttcgg cgaagaccac gcgcgctgca aacgacctcg
      661 gcccgcagtt tgctcctccg caacaatcgc aaggccttcc gccgcgcctt cagacgcacg
      721 tcgcgccagc tcggtgtttg tagaaggcaa cgactcgaat cgcaaaacct tcggcttaag
      781 gaacgtcaca tgagcgatta tacagaaccg agcgcggtag cgccgggtat gcgatcaagc
      841 ttacgctatc tcgaaatacc cgtagtgatg atcgcgttgt tagcgggcgc cgccttcctc
      901 acgaagatgt gctatctctc gctcgccttc aacacgatct ttgtcctgat ttttctaact
      961 ttgttttatt catacgttcg cattcgtctc aacattcaaa tccctctgac attgctggcg
     1021 ctcgtctttc tcgcgttgca agtcgacgca ctcggaaact ttttccgcat gtacggccgg
     1081 cagtttggtc caatgcagta cgacgagttt gcacacacga tggtccaggt gctcgtcagc
     1141 ccgatcatcg tttggctcac cggccgcgtg ctgcaacact tcggctatcg actgccgctc
     1201 aagctcactg cattcttcgc cgccacgatc gtgttcagct tgtcggcgat ctacgagatc
     1261 atcgagttgt gggatgagat ctatttcggc gggcaccgta tctggggacc ttacgacacc
     1321 gccaccgatc tgcagtggga cctctgcgga ataattgtcg ggacactgtt ttcttgtatc
     1381 atgctgcgag acacccgtga tcaactggtt tcccattagg ctttcgttcg tcgtagccac
     1441 agcagcaacg ctgatcgcat tgatcgccgg ctcggggctc gcgtggttgt tagcccgcaa
     1501 gcgatttccc gggcgcaacc tcgtcgatgc gctcgtgacg ctgccgctcg tgttgccgcc
     1561 gactgtgttg ggttattacc tgctggtgtt gctcggcacg cgctcggcaa tcggtggctt
     1621 tctctatcgc acactcggca ttcgactgac gttcacggtg acggcggcga tcattgcggc
     1681 gtcgattcat gcgctgcccc tcgtgacgaa gagtttgcgg gctgcgtttg aaggagtgag
     1741 tacggaactc gaagcggcgg cgcgcacgct cgggttgaat aatcgacgga tcttctttcg
     1801 ggtaacgctg ccgttagcag cgaagggtgt gctggcggcg acggcactcg cgttcgcacg
     1861 tgcgctcggt gatttcggag tgacgatcat gatcgcgggg aacattccgg ggaagacgca
     1921 gactgcttcg gtggcgatct atgatgcggt gcaggcggga cgtgataacg aggcgtttac
     1981 gttggcggtg acggtgtcag tgatcgctgg attcatgctg tacgtgatta atagatttgg
     2041 acgcgtacgt tggtaggttt gtttatccac agattacgca gatgatttat agaatctgtg
     2101 taatctgtgg aaagggttaa ctcaagaagc ctttgaattt tgaggcgctc gatgagtagc
     2161 ctgggaagat tgacttcacg tcagtgctcc ccagatgtcg ggtggcgatc tcgccgaaca
     2221 catctcggaa gtcagtcgtc aacgccagat cacgaccctc gtaaagctga tcgctcttga
     2281 gtcccggcca ctgcccgtaa acttttccgc ccttcacact attacccaag acgaacatcg
     2341 cattcgcatg tccgtggtcc gtgccgcgat tgccgttctc acgcaccgtc cgaccaaact
     2401 cagacatcgt caacacaacc acatcatcca tgcgctggcc cagatcctgg tagagcgccg
     2461 cgataccgct gctgaactgc tgcaacagat tcgccagttg cccacgtgaa ttgccctggt
     2521 taacgtgcgt gtcccaacca ccgatgtcgg tgaacgccac ttccaaaccg acgcccgctt
     2581 tgatgagctg cgcgatctgc aacaacgagg tgccgaacgg attgcgcgga tactgcgcgc
     2641 cattctccgg ttgatactgc gccggattcg ccttcttcaa atagttgact gcttcaaacg
     2701 tctcgcgacc catgcccgcc agcgaatctt tcgtctcgcg cgcgtagatc gcttcgaacc
     2761 cgccctgcag gttttccgat gacttacctg cgcgaatcgc gaagtctgag aggttcgaca
     2821 tcgccaccgc cggcgcgcgt ccctgcaaca cgcgcggcat cgtcttcgtc atcgagactg
     2881 cacgaaacag cgactgatcc gaatcctgtt tactgcgcag ataacgattg agccagccgt
     2941 cactcgtcga cttcactccg ggcgttgccg actccatgta atcctgcgcg tcgaagtgtg
     3001 aacgggtgtt gtccggtgag ccggacgcgt gaatgatcgc gaggcgcttc gagtcccaca
     3061 aaggtttgaa tgcgctcagc gacggatgca gaccaaagaa gccgtcgaga ttgatcgcct
     3121 cagacttcgg aatcgcgatc gagggccgct gctcgtaata gtttgattcg ccaaacggca
     3181 cgacgacgtt cagtccatca accgcaccac gctgaaagat cgcgatcagt gtctttcgtt
     3241 taccactcgt attgccgagt gcgatgcgct cgagaaacga cggcgcggag agcgacatac
     3301 caacactggc gagtgcgacg ctgcctgatt tgagaaaaaa tctgcgattc ataattattg
     3361 cctctgaaac tccggcgtcc ccagaatcaa tccgacaact ttcgtcgccg tatcggacgg
     3421 ctcgagctgc ttcaacaacg cctcacgcgt cgcggctgaa atgtctcccg ccagaagagt
     3481 ctttagatac tcgtccatca ccttcgcctg ctcgccattc gtcttcgcaa gattaaccgt
     3541 cgttccctgc acgcgattgt ttgcgagcgc gagtccgaag ttcattcgct cgagcaatcc
     3601 gccggtgttg acccagctct ccgctgcatc cgaataacca ttcggcgttt ggaacccata
     3661 aagcggctcg cccatgcgct cgatccaacg gtgcgtgccc ggcccgccat tcgtgtcagc
     3721 gccgagcgtt ctgatcgcgc tgatcacgag ctcaaatggt cgcttgatct tcacgcgata
     3781 cgcttcagtc gagttgaatt ctttcgagaa gaagatcgct ttgagcgtct cacgaatatc
     3841 accgtcgctc tttgtgaaag tcgctgccac gcgatcgacg agactctgcg gtggcatgtc
     3901 ggaaacaaaa tgccgcacga gcttggtcgc gatgaacttc gccgtcgatg ggtgatgggc
     3961 gaggatgtcg agtaccgtca gcccgtcttt gattccacca ccggcgggga tcttgtggcc
     4021 gagtacagtc ttctcaccgt cgtcatgcac gcgggcatta aagaagaacg tgcctgcatt
     4081 gcgacgacca gcttcaccca tcatcgcatt cacggccgca ccgccgcccc gcggttgaaa
     4141 gatggtccaa ccagtgaagc agcgcgctac ttcctgcacg tctttctgtg tgtagccacc
     4201 gtcgacgccg agtgtgtgca gctccatcag ctcgcg
//
LOCUS       DASSFQ010000185        14551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1572749, whole genome shotgun sequence.
ACCESSION   DASSFQ010000185 DASSFQ010000000
VERSION     DASSFQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572749"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFH96_06495"
     CDS             complement(<1..948)
                     /locus_tag="VFH96_06495"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973657.1"
                     /translation="MRATTLLLIFTLDVVAQNGVHSADALRSWIALDAPPGWEHIATD
                     PIMRAMPGWKRDVLGNLILRKGSGSPRRVVACGLDRPGFAVTEITDDGYLRLREAGAG
                     RQHSLWVQFHEGQRVRVLTRAGVVPGVVIVKSTHLQGGRVLNAPVATLNDLWVDVGAA
                     SKDEVQRLGIEMLNPVVRDVGGWSYGEFVAGALAGLRVGCAAVASAAQGEVKSGETIF
                     LITTLRSFGFDGLEAALRSLGRVDGVTLVDASDSVGEGISQRLVEKPAYLPESTGLSS
                     VTVLAPRVRFAGTLVESVNVSDASKLLAAVTTVAGVDKEP"
     gene            1235..2419
                     /locus_tag="VFH96_06500"
     CDS             1235..2419
                     /locus_tag="VFH96_06500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET6973658.1"
                     /translation="MKKVLALVVFTGLCIAAFLSAPPAGKAKNDKLRKNANKIENSYI
                     VVLNNDVVGEQGMFSIAPYIADELSNTHKGKLKHIYQNAINGFAVEMSPEDAERLSMD
                     FRVAYVEEDGLMTADTTQTNPPWGLDRIDQRNRPTNGTYVFNHTGAGVFAYVIDTGIR
                     TTHTQFGGRAANVFDAFGGSGADCNGHGTHVSGTIGGSTYGVAKSVNLRGVRVLDCNG
                     SGSTSGVIAGVDFVRTNHSNPAVANMSLGGGASSSLDTAVNNLSNSGVAIAVAAGNSN
                     ANACNSSPARAANAITVGSTTTTDARSSFSNFGTCLDLFAPGSSILSSWFSSDTATAT
                     LSGTSMASPHVAGVAALYKQVNPSASSTTVRNAIVNGATTNVVTNAGTGSPNRLLYSL
                     FF"
     gene            2581..5547
                     /locus_tag="VFH96_06505"
     CDS             2581..5547
                     /locus_tag="VFH96_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6973659.1"
                     /translation="MKLQRTVALVCTFALLATTVLAQQTAAPASKRPLTHQDYDSWRS
                     ILAQQVSRDGKFVAYAYVPQDGDGEIVVRNIATGVDWRAPRGYRPPVPPPDDPGTNLA
                     EFQAEQARLLRPVFTADSRLLVFTTEPTKAEVNKAKKEKKKPEDMPKNGLSIMDLSNG
                     TVTKIERVKTFRVPEDGSGFIAYSLEATPPARPESSPATRTEPSAVAPGNAEPSAKTE
                     STERPSSARSRNKKEYGTDLILRNTTTSTDRKFSDVLDFTISKDAKTLVYTVSSRKED
                     TNGVYAVTTDSDAAPATLLSGKGKYQKLTWDEDNTQLAFTSDKEDADAKPSKFRVYQW
                     NRKDPQATEIVSTTSPGFRKEFVVSERANLNFSLDGSHLFLGTAPPPEPEKNPDEEIP
                     ADEKVLVDLWHWKDDYVQPIQKVRAEQDRNRSYRAVYDLKDKKFVQLADDTMENISPS
                     NDGSYAIGADNRKYRVTNDYDPGFTDYYLVNTSDGSRKPLLTKQRSNVSLSPNAKYAI
                     YFDGKDWYSYSVNGGSTVNLTKDIKVNFYNEENDLPSTPGSYGIAGWTKDDADVLIYD
                     KYDIWRVSPDGSASTNLTDGVGRKESTTLRYVRLDPRERSIDPDKPMLLSAENQETHD
                     SGFYRDKVNATTLPQKLLMTAKDFNSPTKAKDADVLIMTASRFDQFPDVWVTNSAFRE
                     LKRVSNGDAQRAAYTWGTGELVSFKNTDGVPLKGLLLKPENFDPKKKYPMIVYIYEKL
                     SQGLHQFRNPGPGTSINPTYYVSNGYLVFMPDIVYTIGYPGPSALKCVLPGVQAVVDK
                     GYVNENAIGIQGHSWGGYQIAYMVTQTNRFKAAAPGALVANMTSAYSGIRWGTGLPRQ
                     FQYERSQSRIGGSLWDYPLRYLDNSPIFRADRVETPLLMIHNDEDDAVPWYQGIEYFL
                     ALRRLGKEAYMFTYNGEKHGLRKRINQKDYTRRLQEFFDHFLKGAPAPEWMEKGIPYL
                     QREKEKEKYRTSEDKQ"
     gene            complement(5569..6366)
                     /locus_tag="VFH96_06510"
     CDS             complement(5569..6366)
                     /locus_tag="VFH96_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973660.1"
                     /translation="MAGKIKTLIVDDEPLARRNLRVLLERDPQIEILDECRNGREAVK
                     AINTLSPDLIFLDIQMPEMDGFDVLARVGPEHIQAIIFVTAFDQYALKAFDVHALDYL
                     LKPFDDERFARALERAKSQIAAREINQLSERLLALLEERETERKGSKPEERYLTRLMI
                     KASSRMMLLKVDEIDFIEADGNYAKLHAGRKTHLLREKMNDLEGRLDPAKFVRIHRSV
                     IVNLERIKEMHPHFNGDYVVVLEDGRQLRLSRTRREQLEVRLRVNRG"
     gene            complement(6366..7445)
                     /locus_tag="VFH96_06515"
     CDS             complement(6366..7445)
                     /locus_tag="VFH96_06515"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6973661.1"
                     /translation="MKWLGVVAVWAMLGVIYAAPTYIEVRAAQHGHMAWRIFTWGILT
                     WVAWAPLTPVMVWLAQKYSLVGEAWKRNLIVHVPVFLLVSTLHTAAATAVTLWLQPFD
                     NMGESPKTFWPRFLPRVPGSFGSDLLVYGAVIGVFYAIDYYRKYREREVLASRLEAQL
                     AQAQLDSLRNQLHPHFLFNTLNGIVGLVRDNKNTAAVSMLVGLSDLLRHTLEHSSRHE
                     VELREEINFIKLYLSIQEMRFSDRLRIEFDIDPSTTKALVPNLILQPLTENALRHGIA
                     RSENSGLVGIRSVALDGYLRLTVYDEGAGLPDDWQLKGSAGIGLANTIARLQQLYDDD
                     HGFDIRNRDGGGVEVVIVIPFKTAS"
     gene            complement(7594..8745)
                     /gene="moeB"
                     /locus_tag="VFH96_06520"
     CDS             complement(7594..8745)
                     /gene="moeB"
                     /locus_tag="VFH96_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET6973662.1"
                     /translation="MPELSNEEILRYSRHLIMPEVGMQGQRRLKAASVLMIGTGGLGA
                     PVGMYLAAAGVGRIGIVDFDVVEESNLQRQIIHGTKDLGRRKIESARDRLRDINPHIE
                     IETHEARLTSHNAMRLFRNYDIIVDGTDNFPTRYLVNDACVLSGKPNVYGSIFRFEGQ
                     ASIFWAEKGACYRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGAIQANETIKIILGAED
                     LLINRLLLFDAWPMKFREFKLRKDPKCPVCGEQPTIKELIDYEEFCGVAAHQLPQTES
                     KLEEISATELNARRLRGDKIQIIDVREPHEFEIARIPGTKLIPLGHVVSRMNEIDQEV
                     ETIVHCKGGVRSAKAIEALKRAGFQGRLINLKGGISAWSDDVDPRVAKY"
     gene            complement(8788..9048)
                     /locus_tag="VFH96_06525"
                     /pseudo
     CDS             complement(8788..9048)
                     /locus_tag="VFH96_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
     gene            complement(9101..9541)
                     /locus_tag="VFH96_06530"
     CDS             complement(9101..9541)
                     /locus_tag="VFH96_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET6973663.1"
                     /translation="MITISQSLLHEIRAHGVKDYPYECCGLLLGRYGAEGKVVTETYP
                     ISNAREESAKRNRFLIEPVELMRGERYARAHDLEVVGFYHSHPDSPAAPSQYDLEHAW
                     PTYSYIIVKTLAHEATDLFSWEQEPDRTRFNREELRILAADLRG"
     gene            complement(9538..10677)
                     /locus_tag="VFH96_06535"
     CDS             complement(9538..10677)
                     /locus_tag="VFH96_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HET6973664.1"
                     /translation="MTKRDITTKLVHAGERRPLPEAQPVTTPIYASATFTYDTMQEID
                     QVFSGEKQGFIYTRYGNPTTAALEEAVREIEEGAFACAYATGMAAVHAALIACDLKAG
                     STVLASRDVYGATTGLLNTVLSNFGVKTIQADFSDLDAVRETARELKPQVLIAETISN
                     PLLKVCDIEACAQIAHDNGARLIIDNTFATPYLRQPLKLGADIVVHSATKYLGGHADT
                     MGGIVVSRDEMDSLSLISVMKLVGGVLGVFDAHEILRGLKTLAVRMDRQCENARSLAA
                     YLKEHERIGRVHYPGLGALVSIELRDNTKEAAFRFMDALKLVLRSTSLGDVFTSVLHP
                     ATASHRDLLPSKREEWGIVDGLVRISVGIEKLDDIIADIEQALVA"
     gene            complement(10670..11599)
                     /locus_tag="VFH96_06540"
     CDS             complement(10670..11599)
                     /locus_tag="VFH96_06540"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET6973665.1"
                     /translation="MQLDVLETPIPIIEPGRVEAHIGHTPLLRLRLFDKELSPGVQIF
                     AKAEHLNPGGSVKDRAALSMILEGERSGKLKPGMTILDATSGNTGIAYAMIGASRGYA
                     VKVCLPKNASPGRKRILRSYGVEIVETDPMLSTDGAQLIAREIFVENSAKYFYPDQYN
                     NDANWLAHYETTANEIWEQTEGRVTHFVTGLGTSGTFVGVTRRLRELNARLRAIAVQP
                     ESPLHGLEGLKHMATALTPGIFDQKLVTETLEVATEDALLMTSKLARQEGLLVGPSSG
                     ANVFAAWRVAAALREPAVVVTVLCDGGERYLND"
     gene            11852..12739
                     /locus_tag="VFH96_06545"
     CDS             11852..12739
                     /locus_tag="VFH96_06545"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET6973666.1"
                     /translation="MSDWPSLPLEEWKDTCATLHMWSQIVGKIRLAQTPLVNHWWNVP
                     LYVSARGLTTTAMPYEERFFEMEFDFIDHNLVTKCSDGTTAIVPLMPKSVARFYQETM
                     DALETLGIEVKIWKMPVEIADPIPFDQDEQHKSYDREYVERLWSVLRLSERAMQEFRS
                     KFIGKSSPVHFFWGSFDLAVTRFSGRPAPVKPDADPITREAYSHEVISHGWWPGQGPL
                     GKAAYYSYTAPAPDGLGEASVRPEQAFYSKDMGEFLLMYDDVRTAAQPEAALMEFMQS
                     TYEAGADLAKWDRAALERS"
     gene            complement(12788..14419)
                     /locus_tag="VFH96_06550"
     CDS             complement(12788..14419)
                     /locus_tag="VFH96_06550"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HET6973667.1"
                     /translation="MIFQSPAPTPWPSPKGPTPQALFEQYFHDLVNKTWGNISPLYQL
                     DTFDWLILLLYFAILGTLAVYGAYRIKQVIDFWRYKNFVPTPAGHFPEAELPTITVQL
                     PLFNELYVVERLLKAVTAIDYPREKLEIQVLDDSTDETVRVAEAVVAQYAAQGFDIHY
                     IHRADRTGFKAGALENGNKFAKGELLAIFDADFVPKPDCLRKLVDFFTDPMVGCAQMR
                     WSHINGDYNLLTRLQTIMLDGHFVVEQTTRNRTGGFFNFNGTAGIWRRQAIELSGGWQ
                     HDTLTEDTDLSFRAQLMGWKFVYLLDEEAPAEIPVEINAFKAQQRRWAKGVMQVGIKL
                     YPRIWLAPLPFRVKLEMFFRLTGNISYPLMIVASFLQFPLLLVRYNQPFYHLMVLDLP
                     LLFFSSISVVLFYGSAVWYLDKKRAPRLLHLPLVMGLGIGLAFSNARAVLEALLGVKS
                     EFVRTPKYRVEDTADATWKRKKYKRKRGLLPLLELSFAVYFLLAIAYSARLHMWGTIP
                     FLLLFFFGFGYMGLMSLLQTASGKRLAALWRPLKT"
BASE COUNT         3371 a         4308 c         3836 g         3036 t
ORIGIN      
        1 aggttcttta tctactccgg cgactgttgt tacggcggct aataattttg aggcgtcaga
       61 aacattcacg ctttcgacga gcgtcccggc aaagcggacg cgcggcgcca aaactgtaac
      121 agagcttaag cccgtagact ccggcaagta cgccggcttc tcaaccagtc tctgactaat
      181 cccttcacca accgaatcag acgcatcaac gagcgtcact ccatcaacac gcccgagcga
      241 tctcagcgcc gcttccaacc catcgaaacc aaaactgcgc agcgtcgtga tcagaaagat
      301 cgtctcgcca ctcttcacct ctccctgcgc cgcactcgcc accgccgcac aaccaacccg
      361 taaacccgcc agtgcgcccg cgacaaactc accgtacgac catccaccaa catcacgcac
      421 caccggattc aacatctcga tcccgagtcg ctgcacctca tctttcgaag cagccccaac
      481 atcaacccaa agatcgttga gcgtcgcaac cggcgcattc aaaacacgcc cgccttgcaa
      541 gtgcgtgctc ttaacaatca ctaccccagg cacaacaccc gcacgtgtca aaactctgac
      601 ccgctgtcct tcatgaaact ggacccagag cgaatgctgt cgtccggcac cggcctcacg
      661 taaccgcagg taaccgtcgt cagtaatctc agtaacagca aaccctggac gatccaaacc
      721 acacgcaact acacgtctcg gactaccact accttttcgc aaaataagat tgccgagcac
      781 atcacgtttc cacccgggca tcgctcgcat gatcggatca gtggcgatgt gttcccaacc
      841 tggcggcgca tcgagtgcga tccaactgcg aagtgcgtcc gcgctatgaa caccattttg
      901 agcgacaaca tcgagcgtaa atatcaacaa caatgtagtc gcccgcaaca aagttttgac
      961 aggatttgaa ggattcacgg gatggacaag atatacgctt tgaaatactt gcgctacgaa
     1021 aaaattgatg gcatactgcg cccctcccgt cccagaacac tcgtcccata cagtagttag
     1081 ttaagttttc ataacacttc agagtttcct cccagatacg gctggtctat atgtgtcgga
     1141 tttattcaat cgccgattgt ctatcccgct cgttttctcg ccgttgcggg cggctcaacc
     1201 cccaccaacc caactctatc tggaggaaaa aagcatgaag aaagttctcg cgttagttgt
     1261 atttaccggc ctttgcatcg ctgcgttcct gtcagcacca ccagcaggca aagccaagaa
     1321 tgacaagctt cgcaaaaacg caaacaagat tgagaacagc tatatcgtcg tactcaacaa
     1381 cgatgttgtc ggcgagcagg gcatgttctc gatcgcgccg tacatcgccg acgagctgag
     1441 caacacgcat aaaggaaaac tcaaacatat ctaccagaac gccatcaacg gctttgcggt
     1501 agaaatgtca cccgaagatg cggagcggct gagcatggac ttcagggttg catacgttga
     1561 agaggacggt ctcatgaccg ccgacacaac gcaaaccaat ccaccgtggg gcctggatcg
     1621 tattgaccag cgcaatcgcc caacgaatgg aacctacgtc ttcaaccata ccggtgcagg
     1681 cgtctttgcg tatgtcattg ataccggcat tcgcactacc cacacccaat tcggcggacg
     1741 agctgcgaac gtgttcgatg cgttcggcgg cagcggcgcg gattgtaacg gtcacggcac
     1801 gcacgtctct ggcacgatcg gcggatcgac ctacggcgta gccaagagcg tcaacctccg
     1861 tggtgtacgt gtgcttgact gcaacggctc cggctcaact tcaggcgtga ttgccggtgt
     1921 tgattttgtg agaaccaatc actccaatcc cgctgtcgcg aatatgagcc tcggcggcgg
     1981 agcctcgagt tctctcgaca ctgccgtgaa caacctctcg aactcgggag tagcgatcgc
     2041 cgtggccgcg ggtaacagca atgccaatgc atgcaactct tcacccgctc gcgcggcgaa
     2101 tgcgatcacg gtcggctcga cgaccaccac tgacgcacgc tcttcattct caaacttcgg
     2161 cacgtgtctg gatctcttcg cgccgggttc gagcatcttg tcgtcatggt ttagttctga
     2221 cacagcgacc gcaacgttga gtggaacctc aatggcttca ccgcacgttg ccggcgtagc
     2281 agcgctttac aagcaggtta acccgtcagc atcgtcaaca accgttcgca acgccatcgt
     2341 caacggcgcg acgacgaacg ttgtgactaa cgctggtact ggttcaccaa accggctgtt
     2401 gtactcgctg ttcttctaaa agcaaatgtc cgataaactt cagtttgtcg cgcaatccga
     2461 caagctaaag catgtcggac atcaaagtcg ctaagacgta ttgacgcggg tgacacggat
     2521 ctgctctgat ctgcgtcacc cgcgtttttt gtcttagtcc cacaacttat gaggtgcccc
     2581 atgaagcttc aacgaactgt cgctttggtt tgcacgtttg ccttgctcgc gacgacggtg
     2641 ctcgcgcaac aaaccgctgc tcccgctagc aaacgtccgc tcacacacca ggattacgat
     2701 tcgtggcgct cgatcctggc gcaacaggtt tcgcgcgacg gtaagtttgt ggcgtatgcg
     2761 tacgtgccgc aggacggcga cggcgagatc gtcgtgcgca atatcgcgac cggtgtcgat
     2821 tggcgcgcgc caagaggtta tcgtccgcca gtgcccccac ctgacgatcc gggcacgaac
     2881 ctggccgagt ttcaggccga gcaggcgcgt ctgctgcgac cggtgttcac cgcagattct
     2941 cgtttgcttg tcttcaccac tgagcccact aaagccgaag taaacaaagc gaagaaggaa
     3001 aagaagaagc ccgaggacat gccgaagaac ggcctctcga tcatggacct ctcaaacggc
     3061 accgtgacaa agatcgaaag agtaaaaacc ttccgcgtcc ccgaagacgg ctcgggcttt
     3121 atcgcatact cgcttgaagc cacaccgcct gctcgcccgg agtcgagtcc cgcaactcgc
     3181 acagaaccga gcgcggtagc gccgggtaac gcagagccaa gcgctaaaac agaatctacg
     3241 gaacgcccat cctccgctcg ttcacgaaac aagaaggaat acggcaccga tctcatcctt
     3301 cgtaacacca ccacctctac cgatcgcaag ttcagcgacg tcctcgactt cacgatcagc
     3361 aaagacgcaa agacactcgt ctacaccgtc tcatcacgca aagaagacac aaacggagtc
     3421 tacgccgtca caaccgacag cgacgccgca cccgccacac tcctcagcgg caaaggcaag
     3481 tatcaaaaac tgacttggga cgaagacaac acgcagctcg ctttcacgag cgataaagaa
     3541 gacgctgacg cgaagccatc caagtttcgc gtctatcaat ggaaccgcaa agacccgcaa
     3601 gcgaccgaga tcgtctccac cacctcgccg ggcttccgca aagagtttgt cgtgagcgag
     3661 cgcgcaaatc tcaacttctc gctcgacggt agtcacctgt tcctcggcac cgcgcctccg
     3721 cccgagccgg aaaagaaccc cgacgaagaa atccccgccg atgaaaaagt gctcgtcgat
     3781 ctttggcact ggaaagacga ttacgtgcaa ccgattcaga aggtacgcgc cgagcaggat
     3841 cgcaatcgct cgtatcgcgc cgtctacgat ctaaaggata agaaattcgt ccagctcgcc
     3901 gacgatacga tggaaaacat ctcgccctcg aacgacggca gctacgctat cggcgccgac
     3961 aatcgtaaat atcgagtcac gaacgactac gatccaggtt tcactgatta ctatcttgtc
     4021 aacacttccg acggctcgcg caagccactc ttaaccaaac agcgcagcaa cgtctcgctt
     4081 tctcccaatg cgaaatacgc gatctacttc gacggcaaag actggtattc gtattcagtc
     4141 aacggcggct cgacagtaaa tctcaccaaa gacatcaaag ttaacttcta caacgaagag
     4201 aacgacctac catcgacgcc cggctcgtac ggcatcgccg gctggaccaa agacgatgcc
     4261 gacgttctga tctacgataa atacgacatc tggcgcgtct cgccggacgg cagcgcctcc
     4321 acaaacctca ctgacggcgt cggccgtaaa gaatccacca cactccgtta cgttcgactc
     4381 gatccgcgcg agcgctcgat cgatccggac aagccgatgc tgctctcggc tgaaaaccag
     4441 gaaacacacg actccggttt ctatcgcgat aaagtgaacg ccaccacgct cccgcaaaaa
     4501 ctcctgatga ccgcgaagga tttcaacagt ccgacgaagg ccaaagacgc cgacgtgctg
     4561 atcatgacgg cctcgcgctt cgatcagttt ccggatgtct gggtgacaaa cagcgcgttc
     4621 cgcgagttga agcgcgtcag caacggcgac gctcaacggg cagcatatac gtggggcacc
     4681 ggcgagctcg tgagtttcaa aaatactgac ggtgtgcctt tgaaaggact acttctgaaa
     4741 ccggaaaatt tcgatccgaa gaagaagtac ccgatgattg tttacatcta cgagaagctc
     4801 tcgcagggtt tgcaccagtt ccgcaatccc gggccaggca cgtcgatcaa tccgacttac
     4861 tacgtcagca acggctatct agtcttcatg cctgacatcg tttacacgat cggttatccc
     4921 ggaccgagtg cgttgaaatg cgtgttgccg ggagttcagg cggtcgtcga taaaggttac
     4981 gtgaacgaga acgcgatcgg gattcaggga cacagttggg gcggttatca aatcgcttac
     5041 atggtcacgc agacgaatcg cttcaaagcg gcggcgccgg gcgccctcgt cgcaaacatg
     5101 acgagcgctt acagcggcat tcgctggggc acgggattgc caagacagtt tcagtatgag
     5161 cgctcgcagt cgcgcatcgg tggcagcctg tgggattatc cgctgcgcta tctcgacaac
     5221 tcgcccatct tccgcgcgga tcgagtggag acgccgctgc tgatgattca caacgacgaa
     5281 gacgatgcgg tgccgtggta ccagggaatc gagtacttcc tcgccctacg acgtctcggc
     5341 aaagaagcct acatgttcac ttacaacggt gagaagcatg ggttacggaa gcggatcaat
     5401 cagaaggact acacgcgacg cctccaggaa ttcttcgatc acttcctgaa aggcgcaccc
     5461 gcaccggaat ggatggaaaa aggaattccc tatttgcagc gcgaaaaaga aaaggaaaaa
     5521 tatcgaacct cggaagacaa gcagtaaatc gtgttttaag atccgtgttc atccgcgatt
     5581 aactcttagc cggacctcga gctgttctcg tcgggtccgg ctaagtctta attgccttcc
     5641 atcttcgagc acgaccacat agtccccatt gaaatgcgga tgcatctcct tgatccgttc
     5701 gagattgacg atgaccgatc gatgaatccg cacaaacttc gccggatcga gccgcccctc
     5761 gagatcattc atcttctcgc gcagcaaatg cgtcttacgt cccgcatgca gcttcgcata
     5821 attcccatcc gcttcgataa agtcgatctc atcaaccttc aaaagcatca ttcgacttga
     5881 cgccttgatc atcaacctcg tcaggtaacg ctcttcgggt ttcgaccctt tgcgctccgt
     5941 ctcacgctct tccagcaacg ccagcagccg ctcactcaac tggttgatct cgcgcgcggc
     6001 aatctgcgac ttcgcgcgtt cgagtgcgcg cgcaaatcgc tcatcgtcga aaggcttcaa
     6061 caagtagtcg agcgcatgaa cgtcgaaagc cttcagtgca tactgatcga acgcggtaac
     6121 aaagatgatc gcctggatgt gctctggtcc aacacgcgcg agcacgtcga aaccgtccat
     6181 ctccggcatc tggatgtcga gaaagatcag atcgggtgaa agcgtgttga tggcttttac
     6241 tgcctcgcgg ccgttgcgac actcatcgag aatctcgatc tgcggatctc tttcgagcag
     6301 cacacgcaga ttgcgacgcg caagcggctc gtcatcaacg atgagtgtct tgatcttccc
     6361 ggccattacg acgcagtctt aaaaggaatt acgatcacga cttcaacgcc tccgccatct
     6421 cgattacgaa tatcaaaccc gtgatcgtca tcatagagtt gttgcagccg tgcgatagtg
     6481 ttggctaaac cgattccggc gctgcctttc agttgccagt cgtccggcaa gccggcgcct
     6541 tcgtcgtaca cagtcaaccg caggtaaccg tccaacgcaa cagagcggat cccgaccaga
     6601 cctgaattct cactgcgagc aatgccgtga cgcagtgcgt tttccgtgag tggttgcagg
     6661 atcaaattgg ggacgagcgc cttcgtcgtg ctcggatcga tgtcgaactc gatccgcagt
     6721 cgatcggaaa acctcatctc ctgaatactc aaatacagct taatgaagtt gatctcttcc
     6781 ctcaattcga cttcgtgacg cgaagaatgt tcgagcgtgt gacgcaggag atcgctcaac
     6841 ccgacgagca tgctcaccgc cgcagtgttc ttgttatcgc gaacgaggcc gacgatgccg
     6901 ttcagtgtgt tgaagagaaa gtgtgggtgc aactggtttc gcagtgagtc gagctgcgcc
     6961 tgggcgagtt gcgcttctaa tcgcgacgcg agaacctcgc gctcacgata tttgcggtag
     7021 tagtcgatcg catagaagac accgatcaca gcgccataca ccagcagatc agatccaaac
     7081 gatcccggta cacgtggaag gaagcgaggc caaaacgttt tgggactctc tcccatgttg
     7141 tcgaacggct gaagccataa cgtgaccgcc gtggccgccg cagtgtgaag cgttgacacc
     7201 aacagaaaca caggcacgtg aacaataaga ttacgcttcc acgcttcgcc gacgagcgaa
     7261 tacttctgtg cgagccagac catcacgggc gtgagcggcg cccacgcgac ccacgtgaga
     7321 atcccccacg tgaagatgcg ccacgccatg tgcccatgct gtgccgcgcg gacctcgata
     7381 taggtcggcg cggcatagat gacacccagc atcgcccaga cagcaacaac gcccagccac
     7441 ttcatccgca taattgattt tacttatttc ttcacgggtg aggactactt tgtcgcaggt
     7501 tgaagtaagc gagacacaag ccgcctccag catgtgacga actgcatcca cagattacac
     7561 agattgctct gcgtaatctg tgaaatctgt ggactaatac ttcgcaaccc gtggatcgac
     7621 gtcgtctgac caggccgaga ttccgccctt gagattgatc agtcgtccct ggaatccagc
     7681 gcgcttcaat gcctcgattg ccttcgcact acgcacgccg cctttgcaat gaacgatcgt
     7741 ctccacctcc tgatcgatct cgttcatccg gctcacgacg tggcccagcg gaatcagctt
     7801 cgttcccggt atgcgcgcga tctcaaactc gtgcggctcg cgcacgtcga tgatctgaat
     7861 cttatcgcca cgcaagcgac gcgcgttcaa ctcagtcgca gagatctctt cgagcttcga
     7921 ttcagtctgc ggcagctggt gggccgccac tccgcaaaac tcttcgtagt cgatcaattc
     7981 cttgatcgtc ggctgctcgc cgcaaacagg acacttcgga tcctttcgca gcttgaactc
     8041 gcgaaacttc atgggccagg cgtcaaacaa caatagacga ttgatcagca gatcctccgc
     8101 gccgagaatg atcttgatcg tctcgtttgc ctggatcgcg ccgacgatgc ccggcaacac
     8161 gccgagcaca ccaccttccg cgcacgacgg cacgagcccg ggcggcggcg gctccggata
     8221 caaacagcga taacacgcgc ctttctcggc ccagaagatg ctcgcctgtc cttcgaagcg
     8281 gaagatcgag ccgtagacgt ttggcttacc actcaacaca cacgcatcgt tcacgagata
     8341 acgcgtcgga aagttgtcag tgccgtcgac gatgatgtcg tagttgcgga acagtcgcat
     8401 cgcattgtga ctcgtgaggc gcgcctcgtg tgtttcgatc tcgatgtgcg gattgatgtc
     8461 gcgtaaacga tcgcgcgcgg attcgatctt gcgacggccg aggtctttcg tgccgtgaat
     8521 aatctgtcgc tgcagattcg attcttcaac cacgtcgaaa tcaacgatgc cgatgcgacc
     8581 gacgcccgcg gcggcgagat acatgccaac cggcgcaccg agaccgcccg tgccgatcat
     8641 caacacactg gctgctttca aacgccgctg cccctgcatg ccaacctccg gcataatcag
     8701 gtgacggctg tagcgcagga tctcctcgtt tgaaagctcc ggcaacgatt ggcccacttc
     8761 cgactcgatc acagcgccgc cggcaatcga aggaacgatc atgatcgtgt cgccgtcttt
     8821 gactggcgtg tcaggaccgg acgcgtggcg gatgtcttcg tcgttgagat agacgttgac
     8881 gaagttgcgc agcacgttcg gatcgttgaa gagatgtcgg cgcaagccgg tgtgtgttga
     8941 ggtgagcagg tcgagcgcct caccggcggt tgttgcttcg actgtgatct gcgattgtcc
     9001 ccctgcgaat tgccgtagcg gcgccgggat ggtgattgtg attggcatgt tttgacctct
     9061 ttttagccgc ggatctacgc ggattaacgc tgatctgcgt ttatccgcgt aaatccgcgg
     9121 ctaatattct taattcttct cgattgaacc gtgtgcgatc aggctcctgt tcccaggaga
     9181 acaagtccgt cgcttcgtga gcaagcgtct tcacgatgat gtacgagtaa gtgggccacg
     9241 cgtgctcgag atcgtactgc gacggcgccg ccggcgaatc cggatgcgaa tggtaaaacc
     9301 caaccacctc gagatcgtgc gcgcgcgcgt atcgctcacc acgcatcagc tccaccggct
     9361 cgatcaaaaa ccggttgcgc ttcgcactct cctcgcgtgc attcgagatc ggatacgtct
     9421 ctgtaacaac tttgccttcg gcgccgtaac gaccgagcaa cagcccgcag cactcgtacg
     9481 gataatcctt cacgccatgg gcgcggattt cgtgaagtaa actttgggaa atcgtaatca
     9541 tgcgactaac gcctgctcga tatcggcgat gatgtcgtcg agcttttcga tgccaacgga
     9601 tatgcgcacc aaaccatcga cgatgcccca ctcctctcgt ttcgacggta aaagatctcg
     9661 atgcgatgcg gtcgcaggat gcagcacact cgtaaacaca tcgcccaaac tcgtcgagcg
     9721 aagcacgagc ttcaatgcgt ccatgaaacg aaacgccgct tcttttgtgt tgtcgcgcag
     9781 ctcgatcgag acgagcgcac cgagaccggg ataatgaaca cgaccgatac gctcgtgttc
     9841 ttttaaataa gcggcgagcg agcgcgcatt ctcacattgc cgatccatcc gcacggcgag
     9901 cgtcttcaac cctctcagaa tctcgtgtgc atcaaaaact ccgagcacgc cgccgacaag
     9961 cttcatcacg ctgatgagcg agaggctgtc catctcgtcg cgcgatacca cgatgccgcc
    10021 catcgtgtct gcatggccgc ccaggtactt tgtcgcgctg tggaccacga tgtctgcacc
    10081 gagttttaga ggctggcgca ggtaaggcgt cgcgaacgtg ttgtcgatga tcaaacgtgc
    10141 gccgttgtcg tgtgcgatct gagcgcacgc ttcgatgtcg cagactttca gcaacgggtt
    10201 cgagatcgtt tccgcgatga gtacctgtgg tttaagctca cgcgctgttt cacgtaccgc
    10261 atcgaggtcg ctgaaatcag cctgaattgt cttcacaccg aaattagaca acacagtgtt
    10321 cagcagtccc gtggttgcgc cgtagacgtc tcgcgaagca agtacagtcg atccggcttt
    10381 caaatcacac gcgatgagcg ctgcgtggac tgctgccatt cccgtcgcgt atgcacacgc
    10441 gaaagcaccc tcctcgattt cgcgcacggc ttcttccagt gcggccgtcg tcgggttgcc
    10501 gtaacgcgtg taaatgaatc cttgcttctc accactgaag acctgatcga tctcctgcat
    10561 cgtgtcgtaa gtgaacgtcg ccgaggcgta gatcggtgtg gtgacaggct gcgcttccgg
    10621 caacgggcgt cgttcgccgg cgtgcacgag tttggtcgtg atatcgcgtt tagtcattta
    10681 agtaacgttc gccgccgtcg cacaacaccg tcacgacaac tgccggctca cgcaacgccg
    10741 ccgccacgcg ccacgcggca aacacgttcg ctccggagct cggcccgacc agtaatcctt
    10801 cctgacgtgc tagtttactt gtcattaata acgcatcttc ggtagcaact tccagcgtct
    10861 ccgtgaccag cttctggtcg aagattcccg gcgtgagcgc cgtggccatg tgtttcagac
    10921 cttcgagccc gtgcaaaggc gactccggct gcaccgcgat tgctctcagc cgcgcgttca
    10981 attctcgcaa tcgtctcgtc acaccaacaa aagtacccga cgttcctagt ccggttacga
    11041 aatgcgtgac gcgcccttcg gtctgttccc agatctcgtt cgccgtcgtt tcgtaatgcg
    11101 cgagccagtt cgcgtcgttg ttgtactgat cgggataaaa atacttagcg gaattctcga
    11161 cgaagatctc acgcgcgatg agctgcgcgc cgtcggtcga aagcatcgga tcggtctcga
    11221 cgatctcgac gccataactt ctcagaattc gtttccgccc ggggctcgca ttcttcggta
    11281 aacaaacctt caccgcgtaa ccgcgcgacg caccgatcat cgcgtaggcg atcccggtgt
    11341 tgccgctggt tgcatcgagg atcgtcatgc cgggtttgag tttgccgctg cgctcgcctt
    11401 caagaatcat cgagagtgcc gcgcgatcct tcaccgagcc acccggattc agatgttcgg
    11461 ccttcgcaaa gatctgcacg cccgggctca gttccttgtc gaataatcgc agtcgtaaca
    11521 acggcgtgtg accgatatgt gcttcaaccc ggccgggctc aataatgggt ataggggttt
    11581 cgagaacgtc gagttgcata aattgtccaa aaggaaaacg gtcgcaactt ccaggaagtg
    11641 cgaccgtttc gccgtcaaaa ctgctggtgg ttagctcatc tgctatccaa cctgctgcgc
    11701 ggccgctcgg tcgcggtgca gtagcaccac tgacaatagc gacaacatgc gcaggccttc
    11761 atcatgatgg gaaactatag gcccgcgcca atgcagtgtc aagccacgga tccttttgct
    11821 gatccgcatt ttccgcgcta agctttgaca catgtctgac tggccatcat taccactcga
    11881 agaatggaaa gatacgtgtg cgaccctcca catgtggtcg cagatcgtcg gcaaaattcg
    11941 gctcgcgcaa acgccgctcg ttaaccactg gtggaacgtg ccgctttacg tttcagcacg
    12001 tggtctgacg acgacggcga tgccttacga ggagcgcttt ttcgagatgg agtttgactt
    12061 catcgatcac aacctcgtga cgaagtgcag cgatgggacg acggcgatcg tgccgctaat
    12121 gccgaagtct gtggcgcggt tttaccagga aacaatggat gcgctcgaga cgctcggcat
    12181 tgaagtgaag atctggaaga tgccggtcga gattgccgat ccgattccgt tcgatcagga
    12241 tgagcagcac aagtcatacg atcgagagta cgtcgagagg ttatggagtg tgctgcgttt
    12301 gtcggagcga gcgatgcagg agtttcgatc gaaatttatc ggcaagtcta gtccggtgca
    12361 tttcttctgg ggcagcttcg atctcgcggt gacgcgcttc tcggggaggc cggcgccagt
    12421 gaaacccgat gccgatccga tcacgcgtga agcgtattcg cacgaggtga tcagtcatgg
    12481 ctggtggccc gggcagggtc cgctcggcaa ggctgcttac tattcctaca ccgcgcccgc
    12541 gcctgatgga ttgggtgaag cctccgtacg accggagcag gcgttctaca gtaaagacat
    12601 gggcgagttt ttattgatgt acgacgatgt gcgaaccgcg gcgcagcctg aagcggcgct
    12661 gatggaattt atgcagagta cgtatgaggc tggggctgat ttagcgaaat gggatcgcgc
    12721 ggctctcgaa agaagttagc cacggataac acggataaaa atccggattt atccgtgtaa
    12781 tctgtggtta ggtttttagt ggtcgccaca atgccgcgag acgcttgcca ctcgccgttt
    12841 gcaacaagct catcaggccc atgtacccaa acccgaaaaa gaacagcagc agaaacggta
    12901 tcgttcccca catgtgcagc cgcgcggagt aggcgatcgc gagcagaaag taaaccgcaa
    12961 agctcagctc gagcagcggc agcagaccgc gtttgcgttt gtacttcttt cgcttccagg
    13021 tggcgtcggc agtgtcttca acacgatact tcggcgtccg cacaaattcc gacttcacac
    13081 ccagcagtgc ttccagcacc gcgcgcgcat tagaaaacgc gagaccaata ccgagaccca
    13141 tcacgagtgg taagtgcaac aatcgcggcg cacgcttctt atcgagatac caaaccgccg
    13201 agccataaaa caacactacc gagatcgacg agaagaacaa cagcggcagg tcgagcacca
    13261 tcaagtgata aaacggctgg ttgtaacgca ccaaaaggag gggaaactgt aaaaagctcg
    13321 cgacgatcat cagcggataa ctgatgttgc ccgtcaaacg aaagaacatt tccagcttca
    13381 cgcgaaacgg cagcggcgcc agccagatgc gcgggtaaag cttgatgcca acctgcatca
    13441 cgcccttggc ccaacgtcgc tgctgcgcct tgaacgcgtt gatctcgacc gggatttccg
    13501 ccggcgcttc ctcgtcgagc aggtaaacga atttccagcc cattagctgc gcgcgaaaac
    13561 tcagatcggt gtcttcagtc aacgtgtcgt gttgccagcc gccgctcagc tcgattgcct
    13621 gtcgacgcca gatgccggcc gtgccgttga agttgaagaa gccgccagtg cgattgcgtg
    13681 tggtttgttc gacgacgaag tgaccgtcga gcatgatcgt ctgcagacgc gtcaacaggt
    13741 tgtagtcgcc gttgatatgc gaccagcgca tctgcgcgca gccaaccatc ggatcggtaa
    13801 agaagtcgac cagcttgcgc aggcagtcgg gcttcggtac aaagtcggcg tcgaagatcg
    13861 cgagcaactc gcctttggca aacttgttgc cgttctcgag tgcgcccgct ttaaagcccg
    13921 tgcgatcggc gcgatggatg tagtgaatat cgaagccttg cgccgcatat tgagcgacaa
    13981 cggcttcagc gacgcgcact gtttcgtcag tcgaatcgtc gagcacctgg atctcgagct
    14041 tctcgcgcgg atagtcgatc gcggtgacgg ctttcagcag tcgctcgacg acgtacagct
    14101 cgttgaaaag cgggagctgg acggtgatcg tcggcagctc agcttcggga aaatgcccgg
    14161 caggcgtcgg tacaaaattt ttgtatcgcc agaagtcgat gacctgcttg atgcggtacg
    14221 caccgtagac agcgagcgtg ccgaggattg cgaaatacag gagcagaatt aaccagtcga
    14281 aggtgtcgag ctggtaaagc gggctgatgt ttccccaggt tttgttgacg agatcgtgga
    14341 agtactgctc gaaaagggct tgcggcgttg gccccttcgg gctgggccac ggtgtcggtg
    14401 ctggtgattg aaaaatcatg aattacagta cgtccaaaag cgcaagattc taacctaaca
    14461 atcgggcggc ttcaaattcg gcggtcgggg gcgctgtaat cgctgggggc ggccgcgatg
    14521 cgctccggcg aagcgagcgt gaacgttacg g
//
LOCUS       DASSFQ010000186         8623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_231121, whole genome shotgun sequence.
ACCESSION   DASSFQ010000186 DASSFQ010000000
VERSION     DASSFQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231121"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFH96_06555"
     CDS             <1..607
                     /locus_tag="VFH96_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HET6973668.1"
                     /translation="GAAIITTVCSDYGPIPEMKTEATGYGAGTREYPDFPNVLRVLLG
                     ETEAHGATDERLWMLETNLDDASPQIIGHCMDRVLELGALDCFFTPVQMKKNRPGVLL
                     SVLCGRDEKEAVMKLLFTETTTLGVRSYEVARRALQRSVVQVETQYGPIDVKVAHLDG
                     RVVNEMPEFEQCRQAAVKANVPVKIVEEAARLALAKIRERG"
     gene            627..1196
                     /gene="folE"
                     /locus_tag="VFH96_06560"
     CDS             627..1196
                     /gene="folE"
                     /locus_tag="VFH96_06560"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET6973669.1"
                     /translation="MVVDLDRIAQGVRLILEGIGEDPERAGLKETPQRVAEMYAELTS
                     GMREDPSQHVIPLPGDKHDEMVIVKDISIASLCEHHLAPFVGRCHIAYIPKGGRILGI
                     SKLARLAETFSRRLQLQERLTTDIANTLFEGLKPLGVMVVIEAEHTCMTLRGVRKAGA
                     KTVTSALLGGFRSDPRTRAEAMALIKGKD"
     gene            1196..2839
                     /locus_tag="VFH96_06565"
     CDS             1196..2839
                     /locus_tag="VFH96_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6973670.1"
                     /translation="MRRFIFPLVIILIAFGIVFSQQSSALNEATLRAHIKFLSDDLLE
                     GRGTGARGGDLAAKYIAAQLEAVGARGAGENTSFFQPVSLVGVKADPGTVLTISGASG
                     SESFKFADDFVAFTGAQTEAVDVDADLVFVGYGINAPEQKWNDYKGADTDYRGKILVM
                     LVNDPPATTAEPNLFGGKALTYYGRWTYKYEEAARRGAVGAILLHTDQSAGYPWGVVR
                     TSNGSWRFDIARTQNDTTPFLQFRSWVTDDAAHRLMKLAGQDLDALRTKAATRDFQPV
                     KLGLKGKLSLRSEVKRVAAPNVVGVIEGSDPQLKNEYVVYSAHWDHLGIGAPNKDGDT
                     IYNGALDNASGVAVVLAIAETIARMPPAHRPKRSSLFLFPTAEEQGLLGAEWYAKHPV
                     VPLDKTAANVNLDSMNVLGPTQDFIPLGAERSSLKAVVEAVARDMKLRVSADARPEQG
                     SFYRSDHFPFAKAGVPSISLKEGNDYVGRSKEWGEQQFRAYNTAHYHQPSDEMRDTWD
                     FRGMIQEGEIALAIGRMIADAPEKPRFNPGDEFAKKAGN"
     gene            2873..4963
                     /gene="metG"
                     /locus_tag="VFH96_06570"
     CDS             2873..4963
                     /gene="metG"
                     /locus_tag="VFH96_06570"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET6973671.1"
                     /translation="MNTFYVTTPIYYANSLPHLGHLYTMIVGDTISRAKRQRGVDTYF
                     LTGTDEHGINIERAAQKAGRTPKEQADYVVSYYKKMTSEFGLDTAHGGYDIFMRTTKP
                     FHYQGVSELWRRIAKAKTPKGNDAIYKGHYEGWFCAACAEYKTEDEYQKPKNEGDPPT
                     CLIHDTPLDRVSEESYFFRLSDYDEALLSVLEERPDFVRPDYRRNEVASFVRGGLQDL
                     SVSRLKTSVSWGVPVPDDPAHTMYVWFDALTNYITAIGFGNEERQKAVGFEKYWPALH
                     LVGKDILRFHAVYWPAFLLAAGIELPRAIVAHGMWLDPNGRKMSKTLGNTIELDVLQK
                     HFSIDAIRYFCLREMVFGQDGRFGYEGLIDRANSDLASGWGNLSSRTLTMIARYCDSR
                     IPSGLIPEDKLLLAKRTGVDTDETTVAGFIEHARDQFVHHLDNLAFSKALETAWSVVA
                     RVDKMITDAKPWDLAKDENQKQTLGVVLYRAAESLRWLSVLLYPVMPEASLAIWKQLG
                     LDDSPAHIDPTQLKWGELKEGSTIGEVQPVFPRMDKARIMGDINSAALPTEPSAVAPG
                     PPEPSAPVVHATQADAVGVTSFIEIDDFAKVDLRVGQVLSAERIPKADKLLLMKVDIG
                     EEQPRQILAGIAQYYEPEKLIGRKVVIVANLKPRKLRGLESQGMVVAASYGEEGRPVI
                     ATFTEDVPNGARLK"
     gene            5007..5795
                     /locus_tag="VFH96_06575"
     CDS             5007..5795
                     /locus_tag="VFH96_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6973672.1"
                     /translation="MAKKMFIDSHAHIDGHEFDADRDEVIQRAQAAGVSVILNVGTGD
                     PHSGALERAVELGEAHESIYTAIGTHPHDARLYNDSAEAKIKNLINDKHVIAWGEIGL
                     DFHYDNSPRDVQIEVFKRQLRAARECDLPVVIHTREAEAETIEILKSEYDGAQRRGVF
                     HCFSGSMNLARRSIEIGFMISFSGIVTFKKADELRAVAKEVPLDRLLIETDCPYLTPI
                     PYRGKRNEPAYVVEVARALATLHGVDIEDMARITSENFNRFFGL"
     gene            5869..6366
                     /locus_tag="VFH96_06580"
     CDS             5869..6366
                     /locus_tag="VFH96_06580"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET6973673.1"
                     /translation="MAQQNSFDIVSQVDSAEIANALNQTIKEVRQRFDFKGSSANVVH
                     EKNELVLTAEDETRLRNMNDILQQKLVRRGVPLKAFSYGKIEPAAGGTVRQRAAIQEG
                     IPQEKAKEVVKFIKDSKAKVQASIQGDIVRVSGRDRDTLQDIIAKLKDKDFGIHMQFT
                     NYRSN"
     gene            6367..7389
                     /locus_tag="VFH96_06585"
     CDS             6367..7389
                     /locus_tag="VFH96_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6973674.1"
                     /translation="MTTSGNTIALDEEARISARRIFSIIANELMQVELEFERQARSNV
                     QVIDYLGEYLRASGGKRVRPALNILSNYAVGGDGSRYNSIRMATVMEFLHTATLVHDD
                     IIDKADTRRKRPTVNALYGNETAVLMGDWLYMSAFETSLAERSLPVLDILTSVTRKMT
                     EGEILQLTLLGEADVSEAQYFDVLKRKTAYLFSASCEIGAILGGASEKQQTALRDYGL
                     YLGTAFQLIDDLLDFTSSEEALGKAAGADLLGGKVTLPLIYLRDTEPGARVMVETVLR
                     EGQYSSVKPKDLLEAIVRSGALEQTRSRANEYAENACSALEILPDSDYCESLRALPTY
                     ILNRDR"
     gene            7564..8082
                     /locus_tag="VFH96_06590"
     CDS             7564..8082
                     /locus_tag="VFH96_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973675.1"
                     /translation="MHLAARLRELEAEADSVRAELAEMDTLASQTEAAIYRLLSSVLA
                     SGNINLGLPSDAELEAAMRQSQPQSSLRAQIPVERRPQIPTVRNNVEVLSDRFMDRTI
                     PQAVTLLLREEGGPLHVNELYNRLLEGGFKFTGQNPTISVAVSLNRNTRFRKVAPGTF
                     DLVMRDASSKAS"
BASE COUNT         1977 a         2385 c         2492 g         1769 t
ORIGIN      
        1 aggcgcggcg atcattacga ctgtttgcag tgattatggt ccaattccgg aaatgaagac
       61 ggaggcgact ggctacggcg ccgggacgcg tgaatacccg gactttccca acgtcctgcg
      121 cgtgttgctg ggcgagactg aagcacacgg cgctactgac gaacgcttgt ggatgctcga
      181 aacaaacctc gacgacgcgt cgccacaaat catcggtcac tgcatggacc gcgtgctcga
      241 actcggcgcg ctcgactgct tcttcacgcc cgtgcagatg aagaaaaatc gtcccggcgt
      301 gctgctgtca gtgctctgtg ggcgcgatga gaaagaagcg gtgatgaagt tattgttcac
      361 tgagacaacg acgctcggcg tgcgtagtta tgaagtcgcg cggcgcgcgt tgcagcgcag
      421 tgtcgtgcag gtggaaacgc agtatggacc gatcgatgtt aaagtagcgc acctggatgg
      481 gcgtgtcgta aacgagatgc cggagtttga gcagtgccgg caagccgcgg taaaggcgaa
      541 cgtgccggtg aaaatagtgg aagaggctgc tcgattggca ttagcaaaaa ttcgggaacg
      601 cggataaacg cggattgaga acggatatgg ttgttgatct tgatcgcatc gcacagggtg
      661 tgcggctaat tttggaaggg atcggggaag atccggagcg cgcgggcttg aaggagaccc
      721 cgcagcgcgt cgccgagatg tatgcggagt tgacctccgg catgcgcgag gatccgtcgc
      781 agcacgtgat accgctgccg ggtgacaagc acgacgagat ggtgatcgtg aaagacatct
      841 cgatcgcttc gttgtgcgag catcacctcg cgccgtttgt gggtcgttgt catatcgcct
      901 acattccgaa gggcggcagg attctcggga tctcgaagct cgcgcgcctc gcagagacgt
      961 tctcgcggcg cttgcaactg caggagagat taacgacaga catcgcaaat acgttgttcg
     1021 aaggtctcaa gccactcggc gtgatggtcg tgatcgaagc tgagcacacg tgcatgacgt
     1081 tgcgcggcgt gcgcaaggcc ggtgcgaaga cggtgacttc cgcgctgctg ggcggttttc
     1141 ggtccgaccc gcgcacacgc gccgaagcga tggcgcttat taaaggtaaa gactgatgcg
     1201 tcgattcatt tttccattag taatcattct gatcgccttt gggatcgttt tttctcaaca
     1261 atcgtccgcg ctcaacgaag cgacgctccg ggcacatatc aagtttcttt ccgacgattt
     1321 gctcgaaggc cgtggcaccg gcgcgcgtgg tggcgacctc gccgctaaat acattgccgc
     1381 acagctcgaa gccgttggcg caagaggcgc aggtgagaat accagtttct ttcaacctgt
     1441 ttcgctcgtc ggtgtgaaag ccgatcccgg cactgtgttg acgatctcgg gcgcgagcgg
     1501 tagtgagagt ttcaaattcg cggatgactt cgtcgcgttc accggcgccc aaaccgaagc
     1561 cgttgatgtc gacgcagatc tggtattcgt cggatatggg atcaacgcgc cggagcaaaa
     1621 atggaacgac tacaaaggcg ctgacactga ctatcgcggc aagatcctcg taatgctggt
     1681 gaacgatcca ccggcgacga ctgccgagcc gaatctcttc gggggcaagg cgctcactta
     1741 ctacggtcgc tggacttaca agtatgaaga agcggcgcgt cgtggtgcag tgggagcgat
     1801 tctgctgcac accgatcagt cggctggtta tccgtggggc gtagtccgca cgtcgaatgg
     1861 ctcgtggcgc tttgatattg cacgtacgca aaacgacacc acgccgtttc tacagtttcg
     1921 ctcatgggtg actgacgatg ctgcacatcg tttgatgaaa ctcgccggcc aggatctcga
     1981 cgcgctgcgc accaaagccg cgacgcgcga ttttcaacca gtcaaactcg gactcaaagg
     2041 caagctaagt ctcagaagcg aagtgaagcg tgttgcggcg ccgaatgttg tcggcgtgat
     2101 cgagggaagc gatccacagt tgaagaatga atacgtagtg tattcggcgc actgggacca
     2161 tctcggaatc ggcgcaccga acaaggatgg cgacacgatc tataacggcg cgctcgataa
     2221 tgcttccgga gtcgctgtcg tgctggcgat tgcggagacg atcgcgcgga tgcctccggc
     2281 gcaccgtccg aaacgttcca gtctgtttct gtttccgact gctgaagagc agggattact
     2341 gggcgcggag tggtatgcga aacatccggt cgtgccactc gataagactg ccgccaacgt
     2401 aaacctcgac tcgatgaacg tgcttgggcc aacacaggat ttcattccac tcggtgcgga
     2461 gcgttcgtcg ctgaaagcag tggttgaggc agtcgcgcgc gacatgaagt tgcgtgtttc
     2521 ggcagatgcg cgcccggagc agggatcgtt ctatcgctcg gatcattttc cgttcgcaaa
     2581 agccggagtg ccgtcgatca gtttgaaaga aggaaatgat tacgtcgggc gatcgaagga
     2641 atggggcgag cagcagttcc gggcttacaa cacggcgcac tatcaccagc cgtcggatga
     2701 gatgcgcgac acttgggact tccgcggaat gatccaggaa ggtgagatcg cccttgcgat
     2761 tggcagaatg atcgcagatg cgccggagaa gccgcgattt aatcccgggg atgagttcgc
     2821 gaagaaagcc ggaaattagc cacaaaaagg cacaaaaaca caaaactatt taatgaacac
     2881 tttctacgtc accacaccaa tctactacgc caacagcctg ccgcatctcg gtcatctcta
     2941 caccatgatc gtgggcgaca cgatctcgcg tgcaaaacgt cagcgcggcg tcgacactta
     3001 cttcctgacc ggcaccgacg aacacggcat caacatcgag cgtgccgcac agaaggccgg
     3061 gcgcacgccg aaagagcagg ccgattacgt cgtcagttat tacaagaaga tgacgagcga
     3121 gttcgggctc gacaccgcac acggcggcta cgacatcttc atgcgcacca ccaagccgtt
     3181 tcattaccag ggcgtcagcg agctctggcg ccgcattgcg aaagcaaaaa cgccgaaggg
     3241 caacgacgcg atttacaagg gccactatga aggctggttc tgcgccgctt gcgccgagta
     3301 caagactgaa gacgagtatc agaaaccgaa gaacgaaggt gatccgccga cctgccttat
     3361 tcacgacacg ccgctcgatc gtgtctcgga agagagttac ttctttcgac tctcagatta
     3421 tgacgaagcg cttttgtcag tgctcgagga acggcccgat ttcgtgagac ctgattacag
     3481 gcgaaacgaa gtggcgagtt ttgtgcgcgg cgggcttcag gatctgtcag tcagtcgttt
     3541 gaagacttcg gtgagctggg gcgtacccgt gccggatgat ccggcgcata cgatgtacgt
     3601 gtggtttgac gcgctcacga attacatcac cgcgattggt ttcggtaatg aagagcgaca
     3661 gaaggcagtc ggctttgaga agtactggcc ggcgctccat ctcgtcggga aagacatact
     3721 ccgttttcat gcggtctact ggccggcgtt tctgctcgcg gccggcatcg agctaccgcg
     3781 cgcgatcgtg gcccatggca tgtggctcga tccgaacggc cgcaagatgt cgaagacgct
     3841 cggcaacacg attgagctcg acgttctgca aaaacatttt tccatcgacg cgattcgtta
     3901 cttctgcctg cgcgagatgg tgttcggaca ggacggtcgt ttcggttatg agggattgat
     3961 cgatcgcgcg aatagtgatc tggcgagcgg ttggggcaat ctctccagcc gcacgctcac
     4021 gatgattgcg cgttactgcg actcgcgtat tccgtcagga ctgattccgg aagacaagct
     4081 tctgctggcg aagcgcaccg gtgtcgatac tgatgagacg actgtcgccg gattcatcga
     4141 gcacgcgcgc gatcagtttg tgcatcacct ggacaatctc gcgtttagca aagcgctcga
     4201 gacggcgtgg tcggtcgtgg cgcgcgtcga caagatgatc actgacgcga agccctggga
     4261 tctcgcgaag gatgagaatc agaagcagac gctcggagtc gtgttgtatc gcgcggcgga
     4321 atcattgcgc tggttgagtg tgctgcttta tccggtgatg ccggaagcga gcctcgcgat
     4381 ttggaagcag ttaggactgg acgattcgcc cgcacacatc gatccgacac agctcaaatg
     4441 gggcgagttg aaggaaggtt cgacgattgg ggaagtgcag cccgtcttcc cgcgcatgga
     4501 caaagccagg atcatgggag acatcaattc agccgcgctc ccaacagaac cgagcgcggt
     4561 agcgccgggt ccgcctgaac cgagcgctcc agttgttcac gcaactcaag ccgacgccgt
     4621 cggcgtgacc agcttcatcg agatcgacga cttcgccaaa gtcgatctgc gcgtgggcca
     4681 ggtcctttcc gccgagcgta tcccgaaagc cgataaactt ttactgatga aagtcgatat
     4741 cggcgaagag cagccacgac aaatactcgc cggcatcgcg cagtactatg aaccggagaa
     4801 attgatcgga cgcaaagtcg tgatcgtcgc aaacctcaaa ccacgcaagc tgcgtggtct
     4861 cgaatcgcag ggaatggtcg tcgcggcatc atatggcgaa gaaggccgcc cggtgatcgc
     4921 caccttcacc gaagacgtcc ccaacggtgc tcgactgaag tgatcacaaa aggcacagat
     4981 tcatttgtgt ttttctgtgc atttttgtgg ctaagaaaat gttcatcgac tcgcacgcgc
     5041 atattgatgg tcacgagttt gacgctgatc gtgacgaagt aattcaacgc gcgcaggcgg
     5101 cgggtgtttc agtaattttg aatgttggga cgggagatcc gcacagtggc gccctcgagc
     5161 gcgcagttga attaggtgaa gcgcacgaat ccatttacac agccatcggc acgcatcccc
     5221 acgacgcacg cctctacaac gactccgccg aagcgaagat caaaaacctc attaacgaca
     5281 aacacgtcat cgcgtggggc gagatcggtc tcgacttcca ttacgacaac tcaccacgcg
     5341 acgtacaaat cgaggtcttc aaacgccaac tcagagccgc gcgtgaatgc gatctaccag
     5401 tcgtgatcca cacgcgtgaa gctgaagcag aaacaatcga gattttgaaa agtgaatacg
     5461 acggagcaca gcgtcgcggc gtgtttcatt gcttcagcgg cagcatgaat ctcgcccgac
     5521 gttctatcga gatcggtttc atgatctcgt tctcaggcat cgtcactttc aagaaggcag
     5581 atgaattacg tgccgttgca aaagaagtcc cactcgatcg attgctgatc gaaaccgact
     5641 gtccatacct gactccgatc ccgtatcgcg gcaaacgcaa cgagcctgct tacgtcgtcg
     5701 aagtcgcgcg tgccctcgcg acacttcacg gcgtagacat cgaagacatg gcccgcatca
     5761 cgagtgagaa cttcaacagg ttcttcggat tataaggtac tttgtgccgt tttcatctct
     5821 tgtggcaagt ttcacatgct gccgctttga tgttattctc acctgcaaat ggcgcagcag
     5881 aactccttcg acatcgtctc tcaggtcgat tcagccgaga tcgcaaacgc gctcaaccaa
     5941 acgatcaaag aggtgcgcca gcgtttcgac ttcaagggta gctcggccaa cgtcgtgcac
     6001 gagaagaacg agctcgtgct caccgccgaa gacgaaacca ggctgcgcaa catgaacgat
     6061 atcctgcagc agaaactggt ccgtcgtggc gtgccgttga aagcattcag ctacggcaag
     6121 atcgaaccgg ctgcgggtgg cacagtgcga cagcgcgcag cgatccagga aggtattccg
     6181 caggagaaag ccaaagaggt cgtcaagttc atcaaggaca gtaaagcaaa ggttcaggct
     6241 tccattcagg gtgacatcgt gcgcgtgtcc ggccgcgatc gcgacacgct tcaggacatt
     6301 atcgcgaagc tgaaggataa agacttcggc attcacatgc agtttacgaa ctatcgctca
     6361 aactgaatga caacgtcagg caacaccatc gcgctcgacg aagaggcaag gatttcagcg
     6421 cgacgcattt tcagcatcat cgccaacgaa ctgatgcaag tcgagcttga gtttgaacgc
     6481 caggcgcgtt ccaacgtgca ggtgatcgac tatcttggcg agtacttgcg cgcgtcgggt
     6541 gggaaacgcg tgcgacccgc gctcaacatc ctttcaaact atgctgtcgg tggcgatggt
     6601 tcgcgttaca actcgatccg catggcgacg gtgatggagt ttctccacac tgcgacgctg
     6661 gtccatgatg acatcatcga caaagccgac acgcgccgga aacggcccac cgtcaacgcg
     6721 ctttatggaa acgaaaccgc ggtgctcatg ggcgactggc tttacatgtc tgcgttcgag
     6781 acgagtcttg cggagcgatc gttgccggtg ctcgacatcc tgaccagcgt cacgcgcaag
     6841 atgaccgagg gagagatatt gcagctaacg ttgctgggcg aagcagatgt gtcggaggcg
     6901 cagtacttcg acgtactgaa gcgcaagacg gcgtatctgt tcagtgcgag ttgtgagatt
     6961 ggcgcgatac tcggtggcgc gagtgagaag caacagacgg cgctgcgcga ttacgggctt
     7021 tacctcggca cagcgtttca gttgatagac gacctgctcg atttcacctc gagcgaggaa
     7081 gcgcttggga aagctgcggg cgcggacctc ctcggcggca aggtcacttt gccgctgatc
     7141 tatctgcgcg acacagagcc gggcgcgcgt gtaatggttg agaccgtgtt gcgtgaaggg
     7201 cagtacagca gcgtcaaacc aaaggacctg ctcgaagcaa tcgtacgcag cggcgccctc
     7261 gaacagactc ggtcacgggc caacgaatac gcggaaaacg cctgctcggc ccttgagatt
     7321 ctccccgact ccgactactg cgaatctctt cgcgccctcc ccacctacat cctgaaccgc
     7381 gaccgatagt attttagccg cggattcacg cggataaacg cggattgatc tgcgttcatc
     7441 tgcgtgaatc cgcggctaaa aactgttgtc taatctcacg aatccactta gaatcctcga
     7501 ccgatgagcg agaacctgct gtcacgtctg gaacagagtc tgcgacagtc gcgcgcggcg
     7561 aggatgcatt tggccgcacg gctgcgcgag ctcgaggccg aggctgatag cgtgcgcgca
     7621 gagctcgccg agatggatac gctcgcgagc caaacggaag cggcgattta caggcttctt
     7681 tcctccgtgt tggcgtcggg aaacatcaat ctgggattgc cgtcagatgc tgaactcgaa
     7741 gcggccatgc gccagtcgca gccgcagtca tctttgcgcg cacaaatccc ggtggagcgt
     7801 cggcctcaga tcccgacagt ccgcaacaac gtcgaagttc tgagcgaccg cttcatggac
     7861 cgcacgatcc cgcaagccgt gaccctcctg cttcgcgaag aaggtggacc actacacgtc
     7921 aacgagcttt acaaccgtct tctcgaaggc ggcttcaagt tcaccggtca aaacccgacc
     7981 atcagcgtcg ccgtctccct caaccgcaac acccgttttc gcaaagtcgc ccccggcacc
     8041 ttcgacctgg tcatgcgcga cgcctcgtcc aaggcttcct gacaatcaga tactccgcga
     8101 ctcgcgacaa tccttggcac ggtagttcgt gcaaccaagg aatgggcctc gccggccatt
     8161 tttcctgacc agccgaccac cacatccaac tcgcggacat ggcgttgtac cggaggaact
     8221 tgaacccggt gagcgtcgtg gctttcgctg cggccgtcgc gaatctatct ttgttccaca
     8281 atcgtcacat tcgaaataga ttccgtaacg gccgttcttc catgtcattg gctcaacaca
     8341 atcaggacac tcgggattct cggcctcaca aatactcttt tgctgctttc gggcagttgg
     8401 gcttgtgaca taaccgctca cacgagcgca tgccgcaggg ctttgaagtc gcaacatact
     8461 ctcggttgta tctcgttgag aaaaaatatt gagcgaacca tgccagagta tctcctcgtc
     8521 aataattgct atcttctcgt gaatccttgc ccggtagtcg acaggtatac ctaattcacg
     8581 aatatgctta acgacttcag gcaagccttc gtcgagcacg cca
//
LOCUS       DASSFQ010000187         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_84814, whole genome shotgun sequence.
ACCESSION   DASSFQ010000187 DASSFQ010000000
VERSION     DASSFQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84814"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1662
                     /locus_tag="VFH96_06595"
     CDS             94..1662
                     /locus_tag="VFH96_06595"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HET6973676.1"
                     /translation="MSSVQTPIASLSEFAIVVDPKDNVAVVKKATWPGLELILPNESL
                     VEVRGEIPPGHRFATRDITTGEFVLQFGQPIGTSLGIKQGEQITHDNMTDDVPIVREL
                     PEDLHTAPPDYLPIDERGTFMGYRRPNGRVGTRNYVLIVPTSMCASHEASQISMIAEF
                     TLYNRDKYPNVDGVVAIPHNKGCGCQDGSTVDVMLRTLSNYADHPNVGGVILIDLGCE
                     KTNLAQVEKYLLKREKSFDKPVAKIGIQEVGGTQAAIQQGLKEVEQMLPEVNRTAREE
                     CSVSELVLGVKCGGSDGFSGISANPSLGRAADLLVRSGGTVLITEVPEFCGAEHLLAY
                     RAKDVETGRAIYRMVDWYKDYASKFGAVLNNNPSPGNVAGGLLNITIKSLGAIAKAGT
                     SRVEGVIEYAETPAGRGLNLMQGPGYDQESTPGLVAAGATVVVFTTGRGTTIGNAITP
                     VIKLASNTPVFERMSNDLDLSAGGVIDGTETIDEVGQRVFEHVIEVATGVQAKAEIHK
                     HREFQFWAEQTVSL"
     gene            1759..1857
                     /locus_tag="VFH96_06600"
     CDS             1759..1857
                     /locus_tag="VFH96_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET6973677.1"
                     /translation="MKVHWLTDADAALAEARTQKKPVLIDFSAAPA"
     gene            1873..2325
                     /locus_tag="VFH96_06605"
     CDS             1873..2325
                     /locus_tag="VFH96_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973678.1"
                     /translation="MEAESYDDDETAAFIDNNFVPLEAHIKEHPKNFHRFDALWTPSV
                     IILDPNGKERFRIEGYLPKDEFRAQLELALARVAFMSKNWKDAERRYTEVLERYPESK
                     AAPEALYWKGVSHYKATNDHTVLGELPGQFQEKYPDSEWALKTAAWVH"
     gene            2369..3499
                     /locus_tag="VFH96_06610"
     CDS             2369..3499
                     /locus_tag="VFH96_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6973679.1"
                     /translation="MKAQIKLGRIFGVEIGLHYSWFIIALLITFSLAGQFQENNPGWS
                     DGLRWGVSIITAVLFFAAIVVHELSHAIVAKLRGLPVRSITLFALGGVAQIEKEAADA
                     KTEFWMGIIGPITSFVIGVVCLAITVALGWKPPEFPQQPLPAMLMWLGVINIGLAIFN
                     MIPGFPLDGGRVLRGIVWWITGNAVRATRIAARVGQVIAFVMILYGLLQFFRGAGFNG
                     LWMAFIGWFLLSASRESYAQMVISEGLRGLRVGDVMSRDFPVVDAHSNLQTFAEEHLT
                     RTGRRFWVVTLNGQPEGIITPHEINAVPRNRWPYTTVADVMRPLDATHTVNPNTPVTE
                     ALEVMARQDLNQLPVINERGLAGLISRSHILQLIQTRAELHL"
BASE COUNT          803 a          944 c         1058 g          739 t
ORIGIN      
        1 tgatcacgca acttcagacg aggcgattga tgagcaacaa ctgtagggac ggccctccgt
       61 ggccgcccgt gctttaattc ttttaggaaa ctaatgtcga gcgttcaaac gcccatagcg
      121 tctttaagtg agttcgcgat cgtcgtagat ccgaaggaca acgttgccgt agtcaagaaa
      181 gcaacgtggc ccggactgga gctcatactc ccaaacgagt cgctggttga ggtccgcggc
      241 gagattccac cgggccatcg cttcgcgacg cgagacatta cgaccggcga gtttgtgttg
      301 cagtttggac agccgatcgg aacctcgctc ggcatcaagc agggcgaaca gatcacgcac
      361 gataacatga cggatgatgt tccaatcgtt cgcgagcttc ctgaagatct acacaccgcg
      421 ccacctgatt atcttcccat cgatgagcgc ggcacgttta tgggctaccg gcgtccgaac
      481 ggtcgcgtcg gaacacgcaa ttacgtgctc atcgtgccga ccagcatgtg cgccagtcat
      541 gaagcgtcgc agatctcgat gatcgcggag ttcacgcttt acaaccgcga caagtatccg
      601 aacgtcgatg gcgtcgttgc gatcccgcat aacaagggct gcggttgtca ggatggatct
      661 acagtcgatg tgatgctgcg aacgctttcg aattacgcag accatccgaa cgtcggcggc
      721 gtgatactga tcgatcttgg ttgtgaaaag acaaacctgg cgcaggtcga gaagtacttg
      781 ctcaagcgcg agaagtcgtt tgataagccg gtggcgaaga tcgggattca ggaagttggc
      841 ggcacgcagg cagcgattca gcagggactg aaagaagtcg agcagatgtt gcccgaggtg
      901 aatcgtacgg cgcgcgaaga gtgttccgtg agtgagctcg tgttgggggt aaagtgtggc
      961 ggctccgacg gcttttcggg tatctcggca aacccgtcgc tgggtcgcgc tgccgatctg
     1021 ctcgtacgct cagggggaac ggttttgata actgaagtgc ctgagttctg cggcgccgaa
     1081 cacctgctcg cgtatcgcgc gaaagacgtc gagactggtc gcgcgattta tcgcatggtc
     1141 gactggtaca aagactatgc gtcgaagttt ggcgcggtgc tcaataacaa tccgagtccg
     1201 ggtaacgtcg caggcggact gctcaacatt acgatcaaat cgctcggcgc gatcgcgaaa
     1261 gccggaacaa gtcgcgtcga gggcgtgatt gaatacgctg aaacaccagc gggccgcggc
     1321 ttgaatctca tgcagggacc cggctacgac caggaatcga caccgggcct tgttgctgcc
     1381 ggtgcgaccg tcgtcgtctt cacgaccgga cgcggcacca cgatcggcaa cgcgatcaca
     1441 cccgtgatca agctcgcctc aaacactccc gtattcgagc gcatgtcgaa cgatctcgat
     1501 ctctccgccg gcggcgtgat cgacggtacc gaaacgatcg acgaagtagg acaacgcgtt
     1561 ttcgaacacg tgatcgaagt cgcgaccggc gtacaagcca aagccgaaat ccacaaacac
     1621 cgcgaattcc aattctgggc cgaacaaacc gtctctctct aaccagccac aaaaaggcgc
     1681 aaaaaagcac aaagtgaata tgtgcctttt gtgccttttt gcggcaaaat aaaaccactg
     1741 aataggagga cgcgagaaat gaaagtacat tggttaacag acgctgacgc ggcactcgcg
     1801 gaagccagga cacagaagaa gccggtgctc attgacttta gcgccgcgcc ggcatgaggc
     1861 gcatgtgctc ggctggaagc cgagtcatac gacgatgacg agaccgctgc atttattgac
     1921 aacaacttcg tgccgctcga agcacacatc aaagagcatc ccaagaattt ccaccgattc
     1981 gatgccttgt ggacgccgag cgtgatcatt cttgatccaa acggaaaaga acggtttcgt
     2041 atcgaaggtt atttgccgaa agacgagttt cgcgcgcaat tggagttggc tctcgcgcgc
     2101 gtcgcgttca tgagtaagaa ctggaaagat gccgaacgca gatataccga agtgctcgag
     2161 cgttatccgg agtcgaaggc cgcgccggaa gcgctttact ggaaaggtgt gagccattac
     2221 aaagcaacga acgatcacac agtgctcggc gagttgcctg gtcagtttca ggagaaatat
     2281 cctgacagcg agtgggcatt gaaaacagcc gcctgggttc attagcgagg cgggagaacg
     2341 gagcgacgga gcaaggggga gattatcgat gaaggctcaa attaaactcg gccgtatttt
     2401 tggcgttgag atcggcctgc actacagttg gttcatcatt gctcttctga tcacgttctc
     2461 cctcgccgga caatttcagg agaacaatcc gggttggagt gacggcttgc gctggggcgt
     2521 atcaatcatc acggcggttc tgttctttgc cgcgatcgtg gtccatgagc tctcgcacgc
     2581 aattgtcgcg aagctgcgcg gcttgccggt gcgctcgatc acgctcttcg cgctcggcgg
     2641 tgtagcgcag atcgagaaag aagccgccga cgcgaagacg gagttttgga tgggcatcat
     2701 cggtccgatc acgagcttcg tgatcggggt ggtgtgtctc gcgatcactg tcgcgcttgg
     2761 ctggaagccg cccgagtttc cgcaacaacc gctgcccgcg atgttgatgt ggctcggcgt
     2821 catcaacatc gggctcgcga tcttcaacat gattccggga tttccgcttg atggcgggcg
     2881 cgtgctgcgc ggcatcgtgt ggtggatcac gggcaatgcc gtgcgcgcga cgaggatcgc
     2941 ggctcgtgtt ggtcaggtga ttgcgttcgt gatgattctc tacggtttgc tgcaattctt
     3001 cagaggcgcc ggtttcaatg gtctatggat ggcgttcatt ggctggttcc tcctgagtgc
     3061 atcgcgtgag agttatgcgc agatggtcat cagcgaagga ctgcgcggac tgcgtgtggg
     3121 cgacgtgatg tcgcgcgatt ttccagttgt tgatgcacac tcgaatcttc agacatttgc
     3181 cgaggagcat ttaacgcgca cgggacggcg cttctgggtc gtgactctga acgggcagcc
     3241 ggaggggatc atcacgccac acgagataaa cgccgtgccg cggaatcgtt ggccgtacac
     3301 gactgtggca gacgtcatgc gaccgctcga tgcaacacac accgttaatc caaacacacc
     3361 tgtcaccgaa gcactcgagg tgatggcgcg gcaggacctg aatcaactgc ccgtcataaa
     3421 cgagcgtggt ttggcgggac tgatttcgcg atcgcacatc ctgcaactca tccaaacgcg
     3481 cgccgagctg cacctttaat atatttcggc acggtgaata acaactgtag gggtggccct
     3541 ccgt
//
LOCUS       DASSFQ010000188        12892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_85035, whole genome shotgun sequence.
ACCESSION   DASSFQ010000188 DASSFQ010000000
VERSION     DASSFQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12892
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85035"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..378
                     /gene="gatC"
                     /locus_tag="VFH96_06615"
     CDS             82..378
                     /gene="gatC"
                     /locus_tag="VFH96_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET6973680.1"
                     /translation="MPITQSDIEKVAQLAHLELGEEELKTLGPQIAEIVAYVEQLNEL
                     DTTDVEPAIGGLTPEGERTETSRPDEIFGSLGQKIALAEAPDPAEGHFRVPKVL"
     gene            511..1020
                     /locus_tag="VFH96_06620"
     CDS             511..1020
                     /locus_tag="VFH96_06620"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973681.1"
                     /translation="MIAIRSDIYWIAGPWRGQLAILARPRGEDWLEDEVDGWKKAGVG
                     VVVSLLAFSEESELGLTSEAEIVKRSGLTYISSPIADYSVPASKTVMQQLATELNNQL
                     SRGACIGVHCRQGIGRSSLVAACVLVTSGESPQSAFEYIELARGRSVPDTAEQKQWVI
                     SFARDLVTD"
     gene            1026..2420
                     /gene="gatA"
                     /locus_tag="VFH96_06625"
     CDS             1026..2420
                     /gene="gatA"
                     /locus_tag="VFH96_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET6973682.1"
                     /translation="MSRQTVEFSINAAEKLNETLNAFLQIDREGALGRAEEIERSGQG
                     GPLAGIPIAVKDNICVKGMQASCGSKILGDYHPPYNATVVERLTNAGGVVIGKTNCDE
                     FAMGSSNENSAFGPVRNPWDTTRVSGGSSGGSATAVAAGIVPVALGSDTGGSVRQPAS
                     LCGVVGMKPTYGRNSRYGLVAFASSLDQVGVFAREVEDVARVLGVIAGRDPQDATTAD
                     VPVPDYTTSLTGDLKGARIGFPRALFGKGLDDEVKNSVESVVDVYRDLGAEIVDVELP
                     NAGYAIAVYYIIATAEASSNLARFDGVRYGFRAENAPELRQMYRKTRAEGFGAEVKRR
                     IMLGTYVLSAGYYDAYYRKAQQVRTLIKNDFLKAFAGCDAIITPTAPTPAFAIGEKTD
                     DPLAMYLNDIYTVTANLAGIPGISVPCGLSSERLPIGFQLLGPYWSEEMILKLAHAYE
                     QARPFTERPPISAS"
     gene            complement(2407..2721)
                     /locus_tag="VFH96_06630"
     CDS             complement(2407..2721)
                     /locus_tag="VFH96_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973683.1"
                     /translation="MSTFKRRIIRAGGWGVAKRLIKPIPIVGSVFAFGLAGYEIKRKG
                     LIPGAVHVGLDVTPIVGTAKGVLELFTGDLIPDKRKPSTDYTDRSVKKSVKSAQSAVK
                     RR"
     gene            2737..3783
                     /locus_tag="VFH96_06635"
     CDS             2737..3783
                     /locus_tag="VFH96_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6973684.1"
                     /translation="MIACISMRLKLFWLACAIIAIALLLSCRPGSGNTSRLLIYTPHG
                     QDLLKDFVTRYKQQHPQVDIQFLDMGSRQILERVRVEKNRPQADLWWGASHTTFQTAA
                     DENLLLEFRPTWADKVPATARDAGDRWYGTYETPQVIAYNSEAVTEAEAPHDWDDLLD
                     PKWHDKVLIRNPNPSDTMRAIFGAMIWRFYKDTGSTDKGYEWLRKLDANVHEYTADGT
                     LLMQKIARREGLVTMWDMPDVRLHKEQKNFPVGYKIPASGTPLIIDGIAIVRGGPNTE
                     EAKRFYEFVTTPESLAYAAQKYYRIPVRTDVDRAQLPAWMNERFTVMPLDWDLLRKNG
                     GDWLRYWDTEIRKH"
     gene            3839..4912
                     /locus_tag="VFH96_06640"
     CDS             3839..4912
                     /locus_tag="VFH96_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973685.1"
                     /translation="MPLLSLTNISRTFGTTRAVAGVSLDVNQGEFFGLLGPSGCGKTT
                     TLRMIAGLEKPDAGSILFQDKDITNLPPERRGFGMVFQNYALFPHLNVFENVAFGLRA
                     RHKSKEEMNERVNSALELVQLPNYGKRAIDQLSGGQQQRVAIARAIAIEPALLLFDEP
                     LSNLDVSLREETRRELRELVTRLGLTAVYVTHDQEEAFALCDRISVMVGGKLMQTGRP
                     RELYEQPSEISVASFLGRNNLIKAMRLSSSKTTDGEFKTLEGEHTLHLPIKHEDLAPL
                     NKPVYLAIRPEHVELSTNNNTAVNSLHGNIREIVFGGATSTVRVNANGLLIEALVLRT
                     DGLELDQPCNVILSPDHLTLLKD"
     gene            4981..6645
                     /locus_tag="VFH96_06645"
     CDS             4981..6645
                     /locus_tag="VFH96_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET6973686.1"
                     /translation="MALFLITGIVLLYGVIYPNLSVAISSFQRAGSWTLANYTEILSQ
                     RIVLEAIVSSLGLSVATVFFCALVGIPLAFLFERYTFPGRRLFAAFAALPLVLPPLVG
                     TVAFIYLVGESGILAHGIQNLLGLQNPPWRLRGWSALILFHTYTMYPFFYVLTGAGLR
                     RIDASLAEAARSLGASKTTVLRRVLLPQLTPSLLAAALLTFMTSMASFSAPLLFGGDV
                     RVLTLEIFTARQRGDFVIAITETVILAVISLGALFFFQRYEGTRRFAAAAMKGAPRRR
                     ESIASGPARVLATILAIVFAIVLVLPVATLFLVSFAREGSWTTQTIPSAYTVANYQRL
                     FVDPWAAEVFFNSVTMSAIAAVAALLWSFAVVTLLRRGRNEQKFFGEGWRRLLSLLVL
                     VPWALPGTVVAVSVAEAYGGPSLLLGSFVLVGTFWILPVVYFLRFMPLVVRALQASME
                     QVDPTLEEAAGSLGARTWHRFMRVTLPLVWPGAVAGTLLAFVIALGEYVASVLVFVPA
                     NRPISIAIASELRDFNLGAAAAYGVVLIVIISISMIVAGRLERLRG"
     gene            6654..9083
                     /locus_tag="VFH96_06650"
     CDS             6654..9083
                     /locus_tag="VFH96_06650"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6973687.1"
                     /translation="MNLLKNPRNLWLLIPVALLLVLPSAHSQTESDKAELHQALLDLT
                     SPWTVMCVAAHPDDEDGTTLTILRRKYGMHTVSLFSTFGEGGQNAVGPELYEDLGVIR
                     ARETMAAAEVQGSEPHFLGFKDFGFSKSAEEAFRAWGEREILRRMVLQIRLLRPDVII
                     TNHDTTSGHGHHQATGRTVLAAVDAAADPQQFPEQLAQAGTWQVQRLFVRLRGEPAAG
                     GQVVTLDPNETDPVRGTTYAQQALDGLHKHATQGPWPKTVPAGGARPIRYSLVKQAPN
                     SPAVPANAKTPIDGLVLPETVASKLVAPTIETKPLTEFVERRLEVLVALLNAKRRGAF
                     TAPKEVVATDPHRFSLMSSRLDDAIAAAAGASVALNPEDAVLVPGYDARLAAVVTNGG
                     VAEIQIKQLKFTGLGANRNLQAAEKMLPGTDTSVEIKTGVPKNTNLSVPAAEHLYDGR
                     LFGEPLKVDAELSIEGVSFHVETDTRREVAPAVEIVSVDPSPYVTSPATSQRPLDFKV
                     TLKNHLPTEFRGLLRVRGQSLETGREIDLRPGQTETSDLVVRTGLPTGQIQGSSVAIT
                     VDQPNPKDPIAKRDVPLVYADAIVVSGRKVGYLPSYDQTLERSLAALGVDAKKLTVDD
                     IAKSDLSGFNAIIIDNRGYQAHPDLVPVNNRLLKFAGDGGTLLVFYHRTNEWNPNETQ
                     GRPQLAPYPIIIDDSRVTEEDAPVLFLQPRHPLLNFPNKITQKDFENWVQERGLYFPR
                     EWDQQHYAAILSTNDKGEPPLKGGLLVASYGRGNYIYTSYVWYRQLRAGLPGGYRMFA
                     NLISYGRPKED"
     gene            complement(9118..9648)
                     /locus_tag="VFH96_06655"
     CDS             complement(9118..9648)
                     /locus_tag="VFH96_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973688.1"
                     /translation="MFRIKASYAEQMELRTTVERAREFFGELRNFADLMPGIEGIRKE
                     AGGIMRWMVRAEVPVIGAVHAAFAVEKTEDSPERLEWSPARSEMKNYLRYAAVFEERG
                     HKVLIKIVQHVELRRQHARELHRLAILVGEGRISAEMQKRVREMIKTFLERARVKLEA
                     EESLDLDSEGMLDSPA"
     gene            complement(9969..>12892)
                     /locus_tag="VFH96_06660"
     CDS             complement(9969..>12892)
                     /locus_tag="VFH96_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973689.1"
                     /translation="GGPPLQLLVTSVTMGGVPKADANIDFLLRDLPDQHGARLFIERL
                     AKEQPRAHQTLLKQPALLADVLALAAWSPLLATTLEQNPEYLPWLSRERTDPRVRTRD
                     ELKEALARFALMNSSLNPQVLLARFRRRELLRTYLHDIRRSQTLVETMEELSNLADAI
                     LDYALSLARQDLDNRYGLPRRVDDRGRSATAEFCVIALGKLGSMELNYASDIDLVFLY
                     SDDGTTAGTGERGEVSNREYFVKLSETIARIVGQSGGEGAAYRVDLRLRPHGRDGSLA
                     CSLQEAVRYYMKTAQDWERQTLIRSRAAAGSAEFFSRFKAAVTPAVFRPDVSVSGALA
                     SVRLAKQKIDRQVEKKSGYNVKLGRGGIREIEFIAQALQLAHGGKDDWLRVPHTLVSL
                     GRLADRGFITQPERSELSDAYRVLRCLEHRLQMEHGLQTHTVPEAGEAQALLARRMGF
                     SGADQFLHALKTHTTNVRNTYERLFSEVEETAQAKDEIPPVVIDKDAALATAAARILA
                     AHRTERGSAGSTALAHHLIYLARQSLNPHRALLQLSRITASIEKLDHHLVITDANLDE
                     LVQLCGTSDSFAEMIAANPTLIASLGAAPATSLRRDYRAILRTAIDAERTFPAEMAVF
                     RLQWAELLVEIGALDVANELSIFEANRLQTDLATASMNVAYLIARREMARRYGSFSGG
                     PRIAFYGLGRLGSGGVDYGSDLDILITYDSLVPSPIKSLTQDEAYSRLVELMIAALSS
                     ITREGYLYRVDMRLRPHGKNGPLVTSSEGFLDYLKQESAPWEWLAYVKLRSVGGDQEM
                     GKMIETHARHRIHANAEQLDPKELKAVTRHVRERLEKEKGTTRRKLGTDIKYGPGGML
                     DVYFASRYLQLRDEVMDEGDDRSTAFTLDRLREEGSLSEEDFVVLSSGYGLLRSIDHN
                     LRLIVGRSTRLPDPNHPTASDVALRAGFESVAEMQETLTDQMNSVRAAYNRILATDER
                     G"
BASE COUNT         2805 a         3636 c         3706 g         2745 t
ORIGIN      
        1 caacgcgatt cacgacaaag agctgcgaaa aaattttctt aagactgcct tactctcgtt
       61 gaaacgcaag aaataacaca aatgccgata actcaatcag atatcgagaa ggtcgcacaa
      121 ctagcccatc ttgagctggg cgaggaagag ctaaaaaccc ttggcccaca gatcgccgag
      181 atcgttgcct acgtcgaaca actcaacgag ctcgacacca cagacgtcga accagccatc
      241 ggcggcctga cacccgaagg tgaaagaacc gaaacctcgc gccccgacga aatcttcggc
      301 tccctcggcc aaaaaatcgc cctcgccgaa gctcccgacc ccgccgaggg ccacttccgc
      361 gtaccaaagg ttttgtagta taaatccaga caaataatct tttttgaaac gtattttggc
      421 cacagatgaa cacggataaa atccgcggat ttcatccgtg ttcatccgcg gctaaattct
      481 cataccaaca gagtgtgcga agactgattt atgatcgcaa ttagatctga catttattgg
      541 atagcaggac cgtggcgcgg ccagctcgcg atccttgcgc gacctcgcgg tgaagactgg
      601 ttggaagatg aggtcgatgg atggaagaaa gctggcgtcg gcgtcgttgt atctttgctg
      661 gcatttagtg aggaatctga gttgggtctg acaagcgagg ctgagatcgt aaaacgctcc
      721 ggactcacat acatcagctc tccaattgcg gactacagtg tgccggcctc aaaaactgtg
      781 atgcagcagc tcgctactga attaaacaat cagttatctc gcggagcctg cattggtgtt
      841 cactgtcgcc agggcatcgg acgttcgtct ctggtggctg cttgcgtgct agtgacttcc
      901 ggcgaatctc cgcaaagcgc gtttgagtat atcgaactag cacgcggaag atctgttccc
      961 gatacggctg aacagaaaca gtgggtaata tccttcgctc gggatttagt aacggattag
     1021 taagcatgtc gcgtcaaact gtcgaatttt caattaatgc agctgagaag ctgaatgaaa
     1081 cactcaacgc ttttctgcaa atagatcgcg agggggcgtt ggggcgcgct gaggagattg
     1141 agcggagtgg tcagggtggt ccactcgccg gtattcccat cgctgtcaaa gacaatattt
     1201 gcgtaaaagg gatgcaggcg tcctgcgggt cgaagattct cggtgattat catccacctt
     1261 acaacgctac cgtggtcgag cgtcttacta acgccggcgg cgtcgtgatc ggtaagacta
     1321 actgcgacga gtttgcgatg ggctcgtcga atgaaaactc tgcgtttggt ccggtgcgaa
     1381 atccgtggga tacgacgcgt gtttccggcg gctcgtcagg cggctcggca acggctgtcg
     1441 ctgccggcat cgttccggtc gcgctcggtt cagacacggg aggctcagtc agacaaccag
     1501 catcgttgtg cggcgtcgtc gggatgaaac cgacttacgg tcgcaactcg cgctacggtc
     1561 tcgtcgcatt cgcatcgtct ttggaccagg ttggggtgtt tgcgcgcgaa gttgaagacg
     1621 tggcgcgcgt gctcggcgtg atcgccggac gcgatccgca agacgcaacg accgctgatg
     1681 ttccggtacc ggattacacc acttcactga caggcgatct gaagggcgcg cgtatcggtt
     1741 ttccgcgcgc actcttcggc aagggactcg atgatgaagt gaagaattct gttgagagtg
     1801 tggttgacgt ctatcgcgat ctcggcgcgg agattgtcga tgtggagctg ccgaatgccg
     1861 gctacgcaat cgctgtctac tacatcatcg ccacggccga agcctcttcg aatctcgcgc
     1921 gctttgacgg tgtgcgttac gggttccgtg cggagaacgc gccggaactg cggcagatgt
     1981 atcgcaaaac gcgcgcagag ggttttggag cggaagttaa acgtcgcatc atgctcggca
     2041 cgtacgtgct ctccgccggt tattacgatg cctactaccg caaagcacaa caagtgcgga
     2101 cactgatcaa gaacgatttc ctcaaggcgt tcgcgggctg tgatgcgatc atcacgccca
     2161 cggcgccaac gcctgcgttt gcgatcggcg agaagacgga cgatccgctc gcgatgtatc
     2221 tcaatgatat ttatacggtg acggcaaact tggcgggaat tccggggatc agcgtgccgt
     2281 gcgggctttc atctgaacga ctgccgatcg ggtttcagtt gcttgggcca tactggtctg
     2341 aagagatgat tttgaaattg gcgcatgcgt atgagcaagc gcgacccttc acggagcgac
     2401 cgccaatcag cgcctcttaa ccgcagattg cgcagatttc acagatttct ttacagaacg
     2461 atctgtgtaa tctgtggatg gttttctctt gtcagggatc agatcgcccg tgaataattc
     2521 gaggacgccc ttggcggtgc cgacgatagg cgtcacgtcc aggcccacgt gaacggcgcc
     2581 cgggatcaga cccttcctct tgatctcgta gcccgccagc ccgaaggcaa aaaccgaccc
     2641 cacaatcgga atgggtttga tgaggcgctt cgcgacgccc cagccaccgg ccctgatgat
     2701 tctgcgtttg aaggtgctca ttcttaaccc ctaaacgtga tagcatgcat ttcgatgcga
     2761 ctcaagctgt tttggctggc ctgcgcaatt atcgcgattg cgttgctact ttcctgtcgt
     2821 ccgggctccg gtaacacgag ccgcctgctg atctacacgc cgcacggtca ggatctactg
     2881 aaagacttcg tcactcgcta caagcaacaa cacccgcagg tcgatattca gtttctcgac
     2941 atgggctcgc gacagatcct cgagcgtgtg cgcgtcgaga aaaaccgccc gcaagctgac
     3001 ttgtggtggg gcgcgtcgca cacgactttc caaacagccg ctgacgagaa tttgttgctc
     3061 gagtttcgac cgacctgggc cgacaaagtg ccagcgactg cacgagacgc tggtgatcgc
     3121 tggtacggca cttacgaaac gccgcaggtg atcgcataca acagcgaagc agtcacagaa
     3181 gcagaagcgc cgcacgactg ggatgacctg ctcgatccga agtggcacga caaggtcctg
     3241 attcgtaatc ccaatccgtc tgacacgatg cgcgcgatct tcggcgcgat gatctggcgg
     3301 ttctacaaag acaccggctc gaccgacaaa ggttacgagt ggttgcgcaa gctcgacgct
     3361 aacgtacacg aatacactgc tgacggaacg ctcctgatgc agaaaatcgc gcgccgcgaa
     3421 ggactcgtca caatgtggga catgcccgat gttcgtcttc acaaagagca gaagaatttc
     3481 ccggtaggct acaagatacc ggcgagtggt acaccgttga tcatcgacgg catcgcgatc
     3541 gttcgtggtg ggccaaacac ggaagaggcg aaacggtttt atgagtttgt cacgacgccg
     3601 gagagcctgg cttacgcggc gcagaagtat taccgcattc cggtgcgcac cgatgtcgat
     3661 cgcgcgcagc taccggcgtg gatgaacgag cgattcacag tcatgccgct cgattgggac
     3721 ctcttgcgca agaacggcgg cgattggtta agatactggg acactgagat cagaaaacac
     3781 taatgaaacc atccacagat tacgcagaaa tctgtgaaat ctgtggatgg ctttaaagat
     3841 gcctttactc tcgctaacga atatctcccg gacgttcggc acgacgcgcg ctgtcgccgg
     3901 cgtctcactc gacgtcaacc agggcgagtt cttcggcctg cttggtccat ctggctgcgg
     3961 taagacgacg acgctgcgca tgatcgccgg cctcgagaaa cccgacgccg gctcaatcct
     4021 ctttcaagac aaagacatca cgaacctgcc acctgagcga cgcggcttcg gcatggtctt
     4081 tcaaaactac gcgctcttcc ctcacctgaa cgtcttcgag aacgtcgcct tcggtctgcg
     4141 cgcacgtcac aagagcaaag aggagatgaa cgagcgcgtg aacagcgccc tcgagctggt
     4201 ccaactgccc aattacggta aacgcgcaat cgatcagctt tccggtggac aacaacaacg
     4261 cgtcgccatc gcgcgcgcga tagccatcga gcctgccctg ctgttatttg acgagccact
     4321 ctcaaacctc gacgtctcgc ttcgcgaaga gacgcgccgc gagttgcgcg agctggtaac
     4381 acgtctcgga ctaacggcgg tttacgtcac acacgatcag gaagaagcat tcgcactctg
     4441 cgatcgcatc agcgtgatgg tcggcggaaa gctcatgcag acaggtcgcc cgcgcgagct
     4501 ttacgagcaa ccatcggaaa tctcagtcgc gagttttctc ggacggaaca acctgatcaa
     4561 agcgatgcga ctgagctcga gcaaaacaac tgacggtgag ttcaaaacgc tcgaaggcga
     4621 acacacgctg catctgccaa ttaagcacga agacctcgcg ccgctgaaca agcccgtcta
     4681 tctcgcgatt cgcccggagc acgtcgagct cagcacaaac aacaacaccg ccgtgaattc
     4741 gctgcatgga aacattcgcg agatcgtttt tggtggtgcg acatcaaccg tgcgcgtcaa
     4801 cgccaacgga ttgctaatcg aagccctcgt attaagaacc gacggtcttg aactggacca
     4861 gccatgcaac gtgatcctct ccccggatca tttgaccttg ttgaaagatt aagccacgga
     4921 ttgacacgga tgaacacggc tcaagcaaaa aaaagaaacc gtgttcatcc gtggagatcc
     4981 gtggctctat ttctgatcac tggtatcgtg cttctgtatg gcgtgattta cccaaacctc
     5041 tccgtggcca tcagctcgtt tcaacgggca ggatcttgga cgctcgccaa ttacaccgag
     5101 atactctccc aacgaatcgt cctcgaagct atcgtcagca gcctcggact gtcagtcgca
     5161 acagtcttct tctgcgctct cgtcggcata ccgctcgcat ttctattcga acgttacacg
     5221 tttccgggaa gacggttgtt cgccgccttc gcagcccttc ccctggtcct gccaccactc
     5281 gtcggcaccg tcgctttcat atatctcgtc ggtgagtcgg gcatcctcgc gcacggaatc
     5341 cagaacctgc tcggcctgca aaacccaccg tggcgactgc gaggctggtc cgcactaatt
     5401 ctctttcata catacacgat gtacccgttc ttctacgtgc tcacgggcgc cggactgcgc
     5461 cgtatcgacg cgagtctcgc cgaagcagca cgtagcttag gggcatcgaa gacaaccgtg
     5521 cttaggcgcg tcctgttacc gcaactaaca ccatcgctac tcgccgccgc actactcact
     5581 ttcatgacct caatggcgtc gttcagcgcg ccactcttgt ttggcggcga cgtgcgcgta
     5641 ctaacactcg agatcttcac ggcgcgccaa cgcggcgact tcgtcatcgc gatcaccgaa
     5701 actgtgatac tggcggtcat ctcactcggc gcgctcttct tcttccaacg ttacgaagga
     5761 acacgacgat tcgcagccgc cgcaatgaaa ggcgcaccgc gtcgacgcga aagcatcgcg
     5821 agcggaccgg cgcgcgtgct cgccacgatc ctcgcgatcg tcttcgcaat cgtgctggtg
     5881 ttgccggtgg cgacgttatt ccttgtgagt ttcgcgcgcg aaggctcgtg gacgacgcag
     5941 acgatcccat cagcttacac ggtggcaaac taccagcgac tattcgtcga tccatgggcg
     6001 gctgaagttt tcttcaacag cgttacgatg tcggcgatcg cagcggtagc ggcgctgttg
     6061 tggagcttcg cggtcgtcac gttgctacga agaggaagga acgagcaaaa gttctttggc
     6121 gagggatggc ggcgcttgtt gtcactgctg gtgctggttc cgtgggcgtt gccgggaacg
     6181 gtcgtcgcgg tttcggtggc ggaagcttac ggcggaccga gtcttttgct cggctcgttc
     6241 gtgttggtcg gtacgttctg gatcctgccg gtggtttatt tcctgcggtt catgcccctc
     6301 gtcgtgcgtg cacttcaggc cagcatggaa caagtggatc caaccctgga agaagcggcg
     6361 ggctcgctcg gcgcgcgtac ctggcaccga tttatgcgtg tgaccttgcc gctcgtgtgg
     6421 cccggcgcag tcgcgggcac gctgctggca tttgtgatcg cgttaggcga atatgtggct
     6481 tcagtgctcg tgtttgttcc agcgaatcga ccgatctcga tcgcgattgc atctgaatta
     6541 cgagacttca atctcggcgc agcggcggca tacggagtgg tgttgatcgt tatcatctcg
     6601 atcagcatga tcgtagccgg acgtctggag agattaagag gataaaacgg taaatgaatc
     6661 tgcttaaaaa tccgcgtaat ctgtggttac tgattcctgt cgcactactg ctggttcttc
     6721 cctcggcgca ctcgcaaacc gaatccgata aagcagagct gcaccaggcg ctgctcgatc
     6781 tcacgagccc gtggacggtc atgtgtgtgg ccgcgcatcc tgacgacgaa gacggcacca
     6841 cgctcacgat tctgcgccgc aagtatggaa tgcacacggt ctcgctgttc tcgactttcg
     6901 gcgagggcgg gcagaacgcc gtcggtcccg agctgtacga agacctcggc gtgattcgcg
     6961 ctcgcgagac gatggccgcc gctgaagtac aaggctcgga gccgcacttc ctcggcttca
     7021 aggattttgg tttttcgaag tctgctgaag aggcgtttcg cgcgtggggc gagcgagaaa
     7081 tactccgacg catggtgttg cagattcgac tgcttcgtcc cgacgtcatt atcaccaatc
     7141 acgacacgac gagcggacac ggacatcacc aggcgaccgg tcgcactgtc ttggcagcag
     7201 ttgatgctgc tgccgatccg caacagtttc cggaacagct cgcgcaggcg ggtacgtggc
     7261 aagtgcagag actcttcgtt cgcttgcgtg gcgaaccggc tgcgggcgga caagtcgtca
     7321 cactcgatcc taacgaaacc gatccggtgc gcggcacgac ctacgcgcaa caagcactcg
     7381 acggattaca caaacacgcg acgcagggtc cgtggccgaa gacagtcccg gcgggcggcg
     7441 cacgaccgat tcgctacagc ctcgtcaaac aagctccgaa ctcgcctgct gttcccgcaa
     7501 atgcaaagac accgatcgac ggtttagttt tgccggaaac agttgcttcg aaactcgtcg
     7561 ccccgacgat cgaaaccaaa ccgttgacgg agttcgtcga gcgacgtctc gaggtgctcg
     7621 tcgcgctgct caacgcgaaa cgacgcggcg cgttcacagc gccgaaagaa gtggttgcca
     7681 cggatccgca tcgtttcagt ctgatgtcgt cgcgtcttga tgatgcgatt gcggcagcag
     7741 caggtgcgtc agtggcgttg aatccggagg atgcggtgct cgtgcccggt tacgatgccc
     7801 ggctcgcggc agtcgtcact aatggcggcg ttgctgaaat ccagatcaag cagttgaagt
     7861 ttaccggcct cggcgccaac agaaatctgc aggctgcgga gaagatgctg cccggcactg
     7921 acacatcggt ggaaatcaaa accggtgtgc cgaagaacac gaatttgagt gtgcccgcgg
     7981 ctgagcatct ttacgacggg cgattgtttg gcgagccgct gaaagtcgac gctgagttat
     8041 ctatcgaagg agtgtcgttt catgtcgaga ctgacacgcg tcgagaagtc gcacctgctg
     8101 ttgaaatagt cagcgtcgat ccgtcgccgt acgtcacgtc gcctgcaact tcacagaggc
     8161 cactcgattt caaagtcacg ttaaagaacc atctgccgac tgagtttcgt ggcctgttac
     8221 gcgtcagagg tcagagcctg gagacgggtc gtgaaatcga tctccgacca ggccagactg
     8281 aaacatcaga tctcgtagtg cgcacggggc tgccgacggg gcagatccag ggatcgagcg
     8341 tcgcgatcac ggtggaccaa ccgaacccga aagatccgat cgcgaagaga gatgtgccgc
     8401 tggtttacgc ggacgcgatt gtggtgagtg gcaggaaagt cggttacctg ccgagttacg
     8461 atcaaactct tgaacgatcg ctggcggcgt tgggcgtcga cgcgaagaaa ctgactgtcg
     8521 acgacatcgc caagtcagac ttgtcaggat ttaacgccat catcatcgac aatcgtggtt
     8581 atcaggcaca cccggatctg gttccggtga acaatcgtct gctaaagttt gccggggatg
     8641 gtgggacgct actcgtcttt tatcatcgca cgaatgagtg gaacccgaat gagacgcagg
     8701 gccggccgca gcttgcacct tatccgatca tcatcgacga ctcgcgcgtg acggaagaag
     8761 atgcgcccgt gctctttctg cagccacgtc atccgctgct caactttcca aacaagatca
     8821 cgcagaagga ttttgagaac tgggttcagg agcggggact gtactttccg cgcgaatggg
     8881 accagcaaca ctacgccgcc atattgtcga caaacgataa aggcgagccg ccgctaaaag
     8941 gtggactgct ggtggcgtct tacggacgcg ggaattacat ctacacgagt tatgtgtggt
     9001 atcgacaact gcgagcgggc ttgccgggcg gttaccggat gtttgcgaat ttgattagtt
     9061 acggaagacc gaaggaagat taaaacgcgg atctccgcgg attgaaaacg cggatcttta
     9121 agctggtgag tcgagcattc cttctgagtc gagatcgagg gattcttcgg cttccagttt
     9181 tacgcgggcg cgttcgagga atgttttgat catttcgcgg acgcgttttt gcatctcggc
     9241 actgatgcgt ccctcgccga cgagaattgc gagacgatgc agttctctcg cgtgttggcg
     9301 acgcagctcg acgtgctgga caatcttgat cagaactttg tggccacgct cttcaaacac
     9361 agcagcgtag cgcagatagt ttttcatttc ggagcgggcc ggtgaccact cgagacgttc
     9421 cggagaatcc tcggtttttt ccaccgcaaa ggccgcatgg accgcgccaa tcaccggaac
     9481 ttcagcacgg accatccagc gcatgatgcc tcccgcttcc ttgcgtatgc cttcgatgcc
     9541 gggcatcagg tccgcaaaat tacggagttc gccaaagaat tcacgagcgc gttccaccgt
     9601 ggtccgtagt tccatttgtt ctgcgtaaga ggccttgatg cgaaacattt attctccgtt
     9661 aaccgtttct ccgagatgtt gatgcgcgcc ggttccgatt tggtttcccg tattcgcagt
     9721 tgctaagtta cacgaggact gtttggtagt aaatatatat tatgagtgtt aacaacaaat
     9781 gtctgagatg cgacaaaccg ggcctcaaaa cgtgggctga actgaacgac gaggagcgcg
     9841 aggtggtgaa gcgactgccc gcatcagccg ataactctat ggaagaacgc aaggcgtcac
     9901 atcgctggtg cacacggtgt tggaacgagg acttagccgc ggatgaacac ggatgaaatc
     9961 cgcggatttt atccgcgttc atctgtggcc aaaatacgat tgtaggcagc gcgcactgag
    10021 ttcatttgat ccgttagcgt ctcctgcatt tcggcgactg actcgaatcc cgcacgcaaa
    10081 gcgacatcac tcgctgtcgg gtgattcgga tccggcaggc gcgtcgatcg accgacaatc
    10141 aatcgcagat tgtgatcgat cgatctcaat aagccgtacc cactactcaa cacgacaaag
    10201 tcctcttcac tcaaagatcc ctcttcccgc agccggtcga gcgtaaaggc cgtcgagcga
    10261 tcgtcacctt catccatcac ctcatctctg agctgtaaat atcgtgacgc aaaataaaca
    10321 tccagcatgc cgcctgggcc atacttgata tcagtgccga gtttccggcg cgtcgtgccc
    10381 ttctcttttt cgagccgctc acgcacgtgt cgcgtcaccg ctttcagctc tttcggatcg
    10441 agctgctcag cgttcgcgtg tatgcgatga cgcgcatgcg tttcgatcat cttgcccatt
    10501 tcctgatcgc cgccaactga acgcagcttg acgtatgcga gccactccca cggcgcgctc
    10561 tcctgtttca gatagtcaag aaaaccttcc gagctggtga cgagtggtcc atttttgcca
    10621 tgtgggcgca aacgcatgtc gacgcgatag agataacctt cgcgcgtgat gctcgaaagc
    10681 gcggcgatca tcagctccac gagtcgcgag tacgcctcat cctgtgttaa cgacttgatc
    10741 ggtgagggca cgagcgagtc gtaggtgatc aggatgtcga gatcggaacc gtaatcgaca
    10801 ccgccgctgc cgaggcgtcc caaaccgtaa aacgcgatgc gtggaccacc actgaacgag
    10861 ccgtaacgac gcgccatctc gcggcgcgcg atcaggtaag ccacgttcat actcgcggtc
    10921 gcaaggtcgg tctgtagacg attcgcttca aaaattgata gctcgttcgc gacgtccaaa
    10981 gcaccgatct cgacgagcag ctcggcccac tgcaaacgaa acacggccat ctcggcgggg
    11041 aatgtgcgct cggcgtcgat tgctgtgcgc aggatcgcgc ggtaatcgcg tcggagcgat
    11101 gtcgccggcg cagcgccgag tgaggcgatc agcgtcgggt tggcggcgat catttcggcg
    11161 aatgaatcag acgtgccgca gagttggacc agctcgtcga gatttgcgtc tgtaatgacg
    11221 agatgatggt cgagtttttc gatcgaagcc gtgatgcgcg agagttggag cagggcgcga
    11281 tgcggattaa gggattggcg ggctaaataa atcaggtggt gcgccaatgc cgttgacccg
    11341 gcgctaccgc gctcggttct gtgtgctgcg aggatgcgcg cggcggcggt tgcgagggcg
    11401 gcgtctttgt cgatgactac cggtggaatt tcgtctttgg cttgagcagt ttcctcgact
    11461 tcggaaaaca gacgctcgta ggtgttgcgc acgttggtgg tgtgcgtctt taacgcgtgc
    11521 agaaattgat cggcgccaga gaaccccatg cggcgtgcga ggagtgcctg agcttcaccg
    11581 gcttcaggaa ccgtgtgcgt ctgtaaccca tgctccatct gcagacggtg ttcgaggcaa
    11641 cgaaggacgc gataagcgtc tgacagctcg ctccgttcag gctgcgtgat aaagccgcga
    11701 tcggcgagcc ggcccagact cacgagcgta tgcggcacgc gcagccaatc gtccttgcca
    11761 ccatgggcta gctgtagcgc ctgtgcgatg aactcgatct cgcgaattcc gccgcgaccg
    11821 agtttcacgt tgtagccgct cttcttctcg acctgacgat cgatcttctg cttcgccaga
    11881 cgaacactcg cgagcgcgcc actgacagaa acatcgggac gaaatactgc cggcgtcacg
    11941 gctgctttga atcgcgaaaa aaattccgcc gacccagctg cggcgcgcga gcggatcagc
    12001 gtctggcgtt cccagtcctg cgcggttttc atgtagtaac gcacggcttc ctgcaacgaa
    12061 cacgccagcg agccgtcgcg cccgtgcggt cgcagacgca gatcgacgcg atacgccgcg
    12121 ccctcgccgc ccgattggcc cacgatgcgc gcgatcgttt ccgagagctt cacgaagtac
    12181 tcgcgattgc tgacttcacc gcgctcgccc gtgcctgcgg tcgtgccgtc gtctgaataa
    12241 agaaagacga gatcgatatc ggaagcgtag ttgagctcca tcgagccgag cttgccgagc
    12301 gcgatgacgc aaaactcggc agtagcgctg cgaccgcgat cgtcaacacg acgcggcagg
    12361 ccgtaacgat tatcgagatc ctggcgcgcg agactgagcg cgtaatcgag aatcgcgtcg
    12421 gcgaggttcg acagctcttc catcgtctcg acgagtgttt gcgagcggcg aatgtcgtgc
    12481 aggtaagtgc gcaacaattc gcggcgacga aaacgcgcga gcaggacctg cggattcagt
    12541 gatgagttca tcaaagcgaa ccgcgcgagt gcttctttta actcgtcacg cgttctgacg
    12601 cgcggatcgg ttcgctcgcg cgagagccac ggaagatatt cggggttctg ttcgagcgtc
    12661 gtggccagca gcgggctcca cgcagcgagt gcgagaacgt ctgcgagcag cgcgggttgt
    12721 ttgaggagag tctggtgagc gcgcggctgc tctttggcaa gccgctcgat aaaaagacga
    12781 gcgccatgct gatcgggcag atcgcggagc aggaaatcta tattggcgtc agctttgggc
    12841 acgccgccca ttgtaacgga ggtgaccagc aactgtaggg gtggccctcc gt
//
LOCUS       DASSFQ010000189         4676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_822345, whole genome shotgun sequence.
ACCESSION   DASSFQ010000189 DASSFQ010000000
VERSION     DASSFQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822345"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4676)
                     /locus_tag="VFH96_06665"
     CDS             complement(<1..>4676)
                     /locus_tag="VFH96_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET6973690.1"
                     /translation="LLLAGFQALLSRYTGQNDIAVGTDVANRTLLETEDLIGFFINQL
                     VLRTQITPATSFREMLRLVRETTLESYAHQELPFEKLVEELAPERHLDRSPLFQVKLI
                     LQNAPQSDLALGDMNLSQMPIQQPEARFDVVLSLVESPGGIGGNVEYAAGLYDELTIV
                     RMMDHLEVLLESAIAAPDTPVRNLNMTTPAEVNLLLEYGATKVEAQPSRGGVHEMFAQ
                     QVMRTPHAVAVVDQGRPYLYLEVEMRANRLANYLRGIGVGPDVRVGLFMERSLDLAVA
                     MLGVLKAGGAYVPLNQNWPATRIAHVLDDAAIPVLLVHEHLRDRLPMTWATTISIDAD
                     WPDVAAQDAERPSVEVHPEMLAYVIYTSGSTGQPKGVMVSHGALASRVSALAEMYELV
                     PEDRFSQFIAPSFDPFGLEFYPTLSRGASIAFEPNTSEMTGRELVRLLKDREVTVSLI
                     LPAKMEEVTAAVRELPEDERPSLRLLLSGGEAATVAGVAAWHDAFNAPTLHQYGPTEA
                     TILASAYACNESSPVDHETTRSLLGDAIAETQLYVMDEDDELSPLGVSGELYIGGAGV
                     ARGYLNRAGLTAERFVPDLFSGVEGARLYRTGDVVRWRADGELEFLGRRDHQVKLRGY
                     RIELGEIEGVLAQHPLVQQCVVVIRDLGGNATLIAYVVGSVETVELREYLSQQLPAYM
                     VPQRIVSLESLPLTSNGKVDRKALPEPGAKEVERSGQRRARTPMEELLCGIWAEVLGR
                     AEAGIDENFFELGGHSLLATQVVARIRAVFGIEIPLRILFEGPTVEAVAVELGKLKQT
                     QAAPVPELVRVPRDGTLPLSFGQQRLWFLNQLETNNPAYNVQLAVRLDGELDVAALNR
                     ALSEIVRRHEVLRTTFPTVDGIPVQQIAEAVPLSASLIDLRHLDRESGESVASEVAGL
                     EASRPFNLAAGPLLRAMLMRLSDQEHVLVVVMHHIAADAGSLSVLSKEFGLLYEAYRR
                     GLPSPLAELKIQYADYAVWQREWLGEALVNQQLQYWRQQLEGLQHLELPADMPRPAIS
                     TGRGGMVHFEIDQQLAEQLRQLSRREGVTLFMTLTAAFQLLLSKYSGQQDVAIGTVIA
                     NRNRVEIEPLVGLFINQLVLRTRFDRCVNVRDLLGQVRENVLGAYAHQDLPFERLVDE
                     ISPERDLSRSPLFQAMFFYEQHQANVPARTADNAQEDEQHWAKFDLTLMIDDTPAGLG
                     LRLEYAADLFHRSTVERLAKSLCLILETMSAQSQQRLSRLSVIDEAQRLLLTAGYNQT
                     ELPYESRLMHQFFEAQAARTPERTAITCCQRSMSYGELNQRAEALAECLIELGAGPEQ
                     RIAVCFERGPELMVALLAVLKTGSAYVPIDPEYPIDRINYVLEDSEAIIVLTQCHLTA
                     RLPEQERPVLCIDEDVESVEAVSDISPGRCVPANTAYVIYTSGSTGLPKGVAITHDNV
                     ANFFAAMDEKIGPRGEDGKRRWLAVTTVSFDISVLELFWTLGRGFEVVLERAPQQESI
                     AATIREHEITHLQCTPSLGQMLLTEARPADLQSLAALLLGGEALPGAQVAQLRQSYSG
                     PLYNMY"
BASE COUNT         1001 a         1502 c         1212 g          961 t
ORIGIN      
        1 catacatgtt gtagagcggg ccgctgtaac tctgccgcag ttgcgccacc tgcgcgcccg
       61 gcaacgcttc gccacccagc aacaaggctg ccaacgattg cagatctgcc ggtctggctt
      121 cggtcagcag catctggccc aacgacggcg tgcactgcaa atgcgtgatc tcgtgctcgc
      181 gaatcgtcgc tgcgattgac tcttgctgcg gtgcgcgttc caacacaacc tcaaacccgc
      241 gacccaacgt ccagaacaat tccaacactg aaatgtcgaa gctgactgtc gttactgcca
      301 accatctccg ctttccatct tctcctcgtg gtccaatctt ctcatccatc gcggcaaaga
      361 agttcgccac gttgtcgtgt gtaatcgcga cgccttttgg tagtcctgtc gaaccggacg
      421 tgtagatgac ataagccgtg ttagccggca cgcatcgtcc aggtgatata tcgctgacgg
      481 cttcgacact ctccacatct tcgtcgatgc aaagcaccgg gcgctcctgc tccggcagcc
      541 gcgccgtcag atgacactga gtcaagacaa tgattgcttc actgtcttcg agcacgtagt
      601 tgatgcgatc gatcggatac tccggatcga tcggcacata cgcactgccg gtcttaagca
      661 ctgccaatag cgccaccatc aactccgggc cgcgttcgaa acagaccgcg attcgttgct
      721 ctggtccagc acccagctcg atcaggcatt cagccagagc ttcggcccgc tgattcaatt
      781 cgccgtaact catactccgc tggcagcacg tgatggccgt gcgctccgga gtgcgcgccg
      841 cctgcgcctc gaagaactga tgcatcaagc gcgactcata aggtagttct gtctggttgt
      901 aaccagccgt cagcagcagt ctttgtgctt catcaatcac tgacaaccgc gacagtctct
      961 gctgcgactg agcactcatt gtttcgagga tgaggcacag gcttttggcc aaacgctcaa
     1021 ctgtcgaacg atgaaacagg tcagcagcgt attcgagccg caggcccaat cccgcggggg
     1081 tgtcgtcaat cattagcgtg agatcaaact tggcccagtg ctgctcgtct tcctgcgcat
     1141 tgtcagcggt acgcgctggt acgttcgcct gatgttgctc gtagaagaac atcgcctgga
     1201 acaggggcga acgagagaga tcacgctcag gactgatctc gtcgacgaga cgctcaaacg
     1261 gcaagtcctg atgagcataa gcgccgagca cgttctctct gacctggccc aatagatctc
     1321 tgacattcac gcagcgatcg aaacgtgttc gcaacacgag ctgattgatg aacaaaccaa
     1381 ccagcggctc gatctcgacg cgattgcgat tagcaatgac cgtcccgatc gcaacgtcct
     1441 gctgtccgga gtatttgctc agcagcagct ggaaggccgc cgtaagcgtc atgaacaaag
     1501 tgacgccttc gcgccggctt aactgtctga gttgttcggc gagttgctga tcgatctcaa
     1561 agtggaccat gcctccacga ccagtgctga tagcaggccg cggcatgtcg gccggtagtt
     1621 ccagatgctg tagaccttca agctgctggc gccagtactg caactgctga ttcactagcg
     1681 cttcgcccag ccattcgcgt tgccagacgg catagtcagc atactgaatc ttcaactccg
     1741 cgagcggcga cggcaaaccg cgccggtacg cttcgtaaag cagtccgaac tctttgctga
     1801 gcacgctcaa cgaaccggca tcagccgcga tgtgatgcat cacgaccacg agcacgtgct
     1861 cctggtcact cagtctcatc aacatcgctc gcagcagcgg acccgcagcc aggttgaatg
     1921 gtcgactggc ttccaatccg gcaacttcac tcgccacact ctcgccgctc tctcgatcga
     1981 ggtgccgaag atcgatgagt gacgccgata gcgggaccgc ttctgcgatc tgttgaacag
     2041 gaatgccgtc cacggtcggg aaagtcgtac gcaacacttc gtgtcgtcgc actatctccg
     2101 aaagtgcacg gttcagcgct gcaacgtcca actcgccgtc caatcgcact gcgagctgca
     2161 cgttgtaggc aggattgttt gtttccaact ggttgaggaa ccacagtcgc tgctgtccaa
     2221 acgacagcgg cagcgtgcca tcgcgtggca cgcgtaccag ctcaggcacc ggcgctgcct
     2281 gcgtctgctt cagttttcct aactccacag ccactgcttc aacggtcggg ccttcgaaca
     2341 agattcgcag tggtatctct attccaaaga ccgcgcggat gcgcgctacc acctgcgtcg
     2401 ccagcaacga gtgcccaccc aattcgaaga agttctcgtc tataccggcc tctgcgcggc
     2461 ctaacacttc cgcccatatc ccgcacaaca actcttccat cggcgtccgt gcccgtcgct
     2521 gcccgctgcg ctctacttct ttagcacccg gctccggcaa agctttgcga tctactttgc
     2581 cgttactggt caatggaaga ctctccaacg acacgatccg ctgcggcacc atgtaggccg
     2641 gcaactgctg gctcagatac tcgcgcaatt cgaccgtttc cacacttccc acgacataag
     2701 cgatcagcgt cgcattcccg cccagatctc tgataaccac gacgcactgc tgcaccaggg
     2761 gatgctgcgc cagcactcct tctatctcac ccaactcgat gcgatagccc cgcagcttca
     2821 cctggtgatc gcgccgtccg agaaactcca actcgccatc cgcgcgccat cgcaccacgt
     2881 cgccagtccg gtacaaccgt gctccttcca cgcccgaaaa cagatccggc acgaaacgct
     2941 ccgcggtcaa accggcacgg ttcaaataac cacgcgccac gcccgcgccg ccaatgtaaa
     3001 gctcgccact cactcccaac ggactcagct catcatcctc atccatcacg tacaactgcg
     3061 tctcagcaat cgcatctcca agcaaactcc gcgtggtctc atggtccacc ggagaactct
     3121 cgttgcaagc ataagctgac gcgaggatgg tcgcttctgt tggtccatac tgatgcagtg
     3181 tcggcgcatt aaaagcatcg tgccacgccg ccactccggc aaccgttgca gcttcaccgc
     3241 cggaaagcag tagccggagt gacggtcgtt cgtcttcggg taactcacgt acagccgctg
     3301 tcacttcttc catcttcgcc ggcaggatca gcgaaacggt cacctccctg tctttcagca
     3361 gccgcaccaa ctctctcccc gtcatctccg aagtgttcgg ctcaaacgcg atcgaagcgc
     3421 cgcgcgacag agtgggataa aactccaatc caaacggatc aaaagacggc gcgatgaatt
     3481 gtgaaaaacg atcttcaggc acaagctcat acatttccgc caaggcggat acgcgtgaag
     3541 ccaacgcgcc atgcgaaacc atcacgcctt ttggttgacc agtcgatccc gacgtataga
     3601 tcacgtaagc gagcatctcc ggatgcactt caacagacgg acgttccgcg tcctgcgcgg
     3661 caacgtccgg ccaatccgcg tcgatgctga tcgtcgtggc ccacgtcatg ggcaaccggt
     3721 cacgcaagtg ctcgtgtacg agcagcaccg ggatggcagc gtcgtcgagt acgtgagcaa
     3781 tgcgtgttgc cggccagttt tgattaagcg gaacataagc tccgcctgcc ttaagcacgc
     3841 ccagcatcgc cacggccaga tcgaggctgc gttccatgaa caagcccacg cgtacatctg
     3901 gtccaactcc aattccgcgc aggtagttcg ccagacgatt agcacgcatc tcgacttcga
     3961 gatagagata cggtcgtccc tggtccacga ctgcgacagc gtgcggcgtg cgcatcacct
     4021 gttgtgcgaa catctcatga acaccgccgc gactcggttg agcctcaacc ttcgtggcgc
     4081 cgtattcaag tagcaggttg acttcagccg gagtggtcat gttgaggttg cgcaccggcg
     4141 tatcaggcgc agcaatcgcg ctctcgagca ggacctcgag atggtccatc atccgcacga
     4201 tcgtcaactc gtcgtacaaa cctgcggcat attcaacgtt cccgccgata ccgcccgggc
     4261 tttcgaccag cgacaacacc acgtcgaacc gcgcttccgg ttgctggatc ggcatctggc
     4321 tgagattcat gtcgccgagc gcaaggtcgg attgcggagc gttttgcagg atcagcttca
     4381 cctggaatag cggtgagcga tcgagatgac gctcgggcgc aagctcttcg acgagttttt
     4441 cgaatggcag ctcctgatgt gcgtagcttt ccagcgtcgt ctctcggacg aggcgcagca
     4501 tttcacggaa cgaagtcgca ggcgtgatct gcgtacgcaa cacaagctgg ttgataaaga
     4561 acccgatcag gtcttcggtt tcgagcagag tacgattcgc tacgtcggtg ccgactgcaa
     4621 tgtcgttctg tcccgtgtac ctcgagagca gcgcctgaaa gccggcgagg agcaac
//
LOCUS       DASSFQ010000190         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1277932, whole genome shotgun sequence.
ACCESSION   DASSFQ010000190 DASSFQ010000000
VERSION     DASSFQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277932"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2648
                     /locus_tag="VFH96_06670"
     CDS             <1..2648
                     /locus_tag="VFH96_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973691.1"
                     /translation="VTEEPLSTRDLAEDRNVNHANTPEEAGTFSLAGAPAYSNNLTID
                     GLDNNDDRAARERFQPSLEAVAEVQVITNQFSAEYGRASGGRVNLRTRSGSKTFQARA
                     FYFFRDEALNANTFRNNSLGLPRLPLQDHAGGFTLGGPLVNETVFFVSYELNKVLDSA
                     LIDTLVPVKQSHLFPLPAPTDLSRARLEDAEIASEIAPFISSISTPLKNTSTTARVDH
                     QFGPMHNAAIVYLRGRLINLRGFGGGNRLAEALQGRRRNSDAISYSDTFVFSERLVNQ
                     ARFQYSRLAPAFEARGGNSPVVLITLNDPLPSDDPDRRTGTLVAGSSTSGGSDRREER
                     VQVQNVLSFINGDNSLKFGADVHHVRSTFIDLADLSGTFNFASAGDFLAGVPSRFRQN
                     FQSSSTQTNLYTGVFVHDEWQILPQLLLSYGLRYEYETILRDANNFGPRFSTAYSPLD
                     SGKLVFRAGGGIFFNRPLLRTIDDFTLGQQQLFFDTNQLRDPSTGKLMTAAQRRAFIA
                     ANLRFPQTLSVDSPLVRELGVKNTDFSRRLDPHLRIPESYQINAGVERDLGRGYSVEA
                     NFTFTRGIHLWREFNANAPILPAGYKNFTEYLASRDFANFINPATATRPLYSASTAGE
                     LVRFVSGSSDSNTINRSIEFGVPVSVMNLNSRSSTTMLEAALAAISSLRPDPTRGELE
                     QLISVGNSFYRGMTVELRKRFSSGFAFRAGYTLSSLIDDGVVNTSDALVPGDFRAERA
                     RSLLDRRHRFVFSGTFDVRVFQLSPIWRVASGAPFNISIGGVDRNLDDVSNDRPNYSG
                     DLPPIGQVGNLPRNAGRGPGLFVFDLNVTREFQIGRVVVRPSVEFDNVLNKTVFSFGS
                     EFIESPVPTRTMRPREIRVGLKINF"
BASE COUNT          575 a          768 c          790 g          662 t
ORIGIN      
        1 gcgtcactga agagccactc tcgacgcgcg acctcgcgga agatcgcaat gtcaatcacg
       61 cgaacacgcc ggaagaggcg ggcaccttct cgctcgcagg tgcgccggct tactcaaaca
      121 acctgacgat cgacggtctc gacaacaacg acgatcgtgc cgcgcgcgaa cgttttcaac
      181 catcgctcga agcggtggcg gaagtgcagg tgattacaaa ccagttctcg gcggagtatg
      241 gccgggcatc gggcgggcgc gttaacttgc gtacacgcag cggctcgaag acttttcaag
      301 cgcgcgcgtt ttacttcttc cgtgacgaag cgctaaacgc gaatacattt agaaacaatt
      361 cgctggggct gccgagattg cctctacagg accacgccgg cggcttcacg cttggtgggc
      421 cacttgtaaa cgagacagtt ttctttgttt cttacgagct aaacaaagta ttggacagcg
      481 cgctgatcga cacgctcgtg ccggtgaagc agagtcactt gtttccttta cctgcgccga
      541 cagatctgag ccgcgcgcgt ctggaagacg cagaaatcgc ctccgagatc gcgccgttca
      601 tctcgtcgat cagcacgccg ctaaaaaaca caagcaccac cgcgcgcgtc gatcaccagt
      661 ttggaccgat gcacaatgca gcgatcgttt atctacgcgg acgactgatc aacttacgtg
      721 gttttggcgg cggcaatcgc ctcgctgaag cattgcaggg ccggcgacgc aattccgacg
      781 cgatctcgta ttccgacacg ttcgtcttct cagaacgact cgtgaaccag gcgcgctttc
      841 aatactcacg tctcgcgccg gcgttcgagg ctcgcggagg caattcgcct gtcgtcctta
      901 tcacgctcaa cgatccgttg ccgtccgacg atccggatcg acgcacaggg actttagtcg
      961 cgggctcgtc aacgtcaggc gggagcgata gacgtgaaga acgcgtacag gtgcaaaacg
     1021 tgctttcgtt catcaacgga gataactcgc tgaaattcgg tgctgacgtt catcacgtgc
     1081 gctcaacctt catcgatctc gcggatctca gcggcacatt caacttcgcg agtgccggtg
     1141 attttcttgc gggtgttccg agccggttcc ggcagaactt ccagagctca tcaacgcaga
     1201 cgaatctcta cacgggcgtg ttcgtgcacg atgagtggca aatcctgccg cagcttttgc
     1261 tgagttatgg attgcgttac gagtacgaga cgatcctgcg cgatgcgaac aacttcggcc
     1321 cgcgcttttc cactgcgtac agcccgctcg actccggcaa gctcgtgttt cgtgcaggcg
     1381 gtgggatctt tttcaatcgc ccgctgctgc ggacgatcga tgatttcacg ctcggacagc
     1441 agcaactctt tttcgacacg aaccagcttc gcgatccgtc aacgggaaag ttgatgactg
     1501 ccgctcagcg acgtgcgttc atcgctgcga atctgcgctt tccgcaaaca ttgagtgttg
     1561 attcaccgct ggtccgtgag ctcggcgtaa agaacactga tttttcacgt cgcctcgatc
     1621 cgcacttgcg aattccggaa agttatcaga tcaatgccgg tgtcgagcgc gatctcggtc
     1681 gcggttattc cgtcgaagca aacttcacct tcacgcgtgg catacacctt tggcgcgagt
     1741 tcaacgcgaa cgcgccgatt cttccggccg gctacaaaaa cttcaccgag tatctggcgt
     1801 cgcgtgactt cgcgaacttc atcaatccag cgactgcgac gcgtccgctt tatagcgcgt
     1861 caaccgcggg ggagctcgtg aggtttgttt ccggcagctc agattcaaac acaataaatc
     1921 gctcgatcga gtttggcgtt cccgtttcag tgatgaatct caacagtcga tcgtcgacaa
     1981 cgatgctcga agccgcgctt gctgcaatta gctcgttgcg tccggatcct acgcgcggcg
     2041 agctcgagca actgatctcc gttggaaaca gtttctatcg agggatgact gtcgagttgc
     2101 gaaagcgatt cagttcggga ttcgcctttc gcgctggtta cacgctttcg agtttgattg
     2161 acgatggggt agtcaatact tcggacgcgc tcgtgcccgg agactttcgt gcggaacgag
     2221 cgcgcagttt gctcgaccgg cggcatcgct ttgtcttttc cgggacgttc gatgttcgtg
     2281 tgttccagtt gtcgccgatc tggcgtgtcg cttcgggtgc gccgttcaac atctcgattg
     2341 gcggtgtgga tcggaatctt gatgacgtct caaacgatcg accgaactat tcgggagatc
     2401 tgccgccgat tgggcaagtt gggaatttgc cacgcaatgc tggtcgtggg ccgggattat
     2461 tcgtgttcga tctgaatgtg acgcgcgagt ttcagattgg gcgcgttgtg gtgaggccgt
     2521 cggttgaatt cgacaatgtc ttgaataaga ccgtgtttag tttcggctct gagttcatcg
     2581 agtcgccggt accgacgagg acgatgagac cgagggagat tcgcgttggt ttgaagatca
     2641 atttttagcc gcggatgaac gcagatgaac gcggatctta aatactttgc actgacgcag
     2701 gagattattg gtgtctacta tgaggtttat aacgagctgg ggacgggatt cttagagtct
     2761 gtataccaaa aatctctggc gttggcgctc gaatc
//
LOCUS       DASSFQ010000191         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_982965, whole genome shotgun sequence.
ACCESSION   DASSFQ010000191 DASSFQ010000000
VERSION     DASSFQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982965"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2390
                     /locus_tag="VFH96_06675"
     CDS             <1..2390
                     /locus_tag="VFH96_06675"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HET6973692.1"
                     /translation="QFALFWNQPSTNVYDPIFGKSLGFYLFSLPLYDLLSSWLLGVTF
                     IILIAAVLYSLLGLPQTVLKPSVRWSSGAAFRGVCCALALFFLVVSWHTYLSRFPFLW
                     EDHATISGVTYTEAHYTLPALLFVCVALIIAAIILIVNAFAMRRFSLLLLAIALPVAV
                     YIVGVLLVPSYIQSFIVKPNEIDRESPYIGYNIEWTRKGFGLDQIELRDFEAENSTAA
                     LDLPNNRESLQNIRLWDWRALQDTLRQIQAIRTYYDFPDVDVDRYVTGGTTRQMMIAP
                     REINDSKLPSQSRNWINERLIYTHGYGVTMNSANGFTPEGLPQFMLSNMPVESTAPEI
                     KVTRPEIYFGELTDRYVYVKTKQNEFDYPQGEINAVTTYQGTGGIRVGNRLRRMLLAW
                     GIGDVSKLPFSNDVTSDSRVLINRNIRDIVNGVAPFLTYDKDAYIVVSNEGRLFWMLD
                     AFTESDYFPYSTHHDVAGNSLNYIRNSVKVVIDAYNGTANFYVFDNQDPIINAYSRVF
                     PNLFKDASQMPADLRAHVRYPETLVRAQGEVYSLYHTQNPKVFFQREDVWSFAQQNIV
                     TAEGKKENAPIDPYYVMMQLPGEQQKNEFVLIVPFTPANRNNMIGWMAGRSDGEHYGK
                     LVVYNFPKSRLIDGPVQIEARIDQNAQLSAQFSLWNQQGSRVIRGHLLVIPLGRSLMF
                     VEPVYLQAERSPMPELRLVVLATQEKLGYGQTFGEAMNSLFGEAAVKPPAEEPAQPTP
                     EQPTPTADLQQLINRAIQEFEDYQRLTSQGKLGEAGQKLEQHKRTLEEIRQKSKP"
     gene            complement(2403..>2831)
                     /locus_tag="VFH96_06680"
     CDS             complement(2403..>2831)
                     /locus_tag="VFH96_06680"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET6973693.1"
                     /translation="PEGHFWLGANYGGSAAHSTLSNLSSFQDIKGEMEAVLKLDEGYQ
                     GYSAYLGLGRLYLQAPKMLGGDPAKAVEYLEKGVKFNPNNTLMRYELAAAYEAVNRNG
                     DAKKQIETLMSTTPDPKYVAEHKQAVEDAKKLLQKIESES"
BASE COUNT          594 a          813 c          774 g          650 t
ORIGIN      
        1 gccagttcgc actcttctgg aaccagccgt caacgaacgt ttacgatccg atctttggca
       61 agtcgctcgg tttctatctt ttttcgctgc cgctttacga tctgctgagc tcgtggctgc
      121 tcggtgtgac gttcatcatc ctgatcgcgg cggtgcttta ctcgctgctt ggtctaccgc
      181 aaaccgtgct gaaaccgagc gtgcgttggt cgtcgggcgc agcgttccgc ggtgtctgtt
      241 gcgcgcttgc actctttttc ctggtggtga gctggcacac gtatctctcg cgcttccctt
      301 tcctctggga agatcatgcg accatttccg gagtgactta caccgaagct cactacacgc
      361 ttcccgcgct cctgttcgtc tgtgtggcgt tgatcatcgc cgcgatcatt ttgatcgtta
      421 acgcgtttgc catgcggcgc ttcagcttgt tgctgctcgc tatcgcgctt ccggtcgcgg
      481 tttacattgt cggcgtgctg ctggtgccgt cgtatatcca gagcttcatc gtcaaaccga
      541 atgagatcga ccgcgagtca ccttacatcg gttacaacat cgaatggacg cgcaaaggct
      601 ttggtctcga tcagatcgag ctgcgcgact tcgaagcgga gaattcaacc gccgcactcg
      661 atctgcccaa caatcgcgaa agcctccaga acatccggct ttgggactgg cgcgcgctgc
      721 aagacactct ccgacaaatt caggccatcc gcacttatta cgactttcct gacgtcgacg
      781 tagatcgcta cgtcaccggc ggcactacgc gccagatgat gatcgcgccg cgcgagatta
      841 acgactccaa gcttccgtca cagtcgcgca actggatcaa cgagcggctg atctacacgc
      901 acggttacgg cgtgacgatg aatagcgcga atggcttcac gcccgagggc ctgccgcagt
      961 ttatgttgtc gaacatgccg gtggagtcga ctgcacccga gatcaaagtc acgcgtccgg
     1021 agatctattt cggcgagctc acggatcgtt acgtctacgt gaagacgaag cagaacgagt
     1081 ttgactaccc gcaaggcgag ataaacgccg tcacgactta ccagggcacg ggcggaattc
     1141 gcgtcggaaa ccgtttgcgc cgcatgctac tcgcgtgggg catcggcgat gtctcgaagc
     1201 tgccgttctc taacgatgtg acgagcgaca gccgtgtgct gatcaatcga aacattcgcg
     1261 atatcgtcaa cggtgtcgct ccgtttctga cttacgataa agacgcgtat atcgtcgtct
     1321 ccaatgaagg ccggctgttc tggatgctcg atgcttttac cgagtcggat tactttccgt
     1381 attccacaca tcacgacgtc gccggaaact cgctcaacta cattcgcaac agcgtcaaag
     1441 tcgtgatcga cgcatacaac ggcacggcta atttctacgt gtttgataat caggatccga
     1501 tcatcaacgc ttacagtcga gtctttccga atctcttcaa ggacgcgagc cagatgcctg
     1561 ccgatctgcg ggcgcacgtt cgctatccgg aaacactcgt gcgcgctcag ggcgaagtct
     1621 acagccttta tcacacgcaa aacccgaaag tgtttttcca acgcgaggat gtttggagct
     1681 tcgcgcagca gaacatcgtc actgctgaag gcaagaaaga gaacgcgccg atcgatcctt
     1741 attacgtgat gatgcagttg ccgggcgagc agcagaagaa tgagtttgtg ctgatcgtgc
     1801 cgttcacgcc ggctaaccgg aacaacatga tcgggtggat ggcgggccgc agtgacggcg
     1861 agcactacgg caaactggtc gtctataact tcccgaagtc tcgcctgatc gatgggccag
     1921 tgcagatcga agcacgcatc gatcagaacg cgcagttatc ggcccagttc agtctttgga
     1981 accagcaagg ctcgcgtgtg attcgtgggc atctgctggt gataccgctg ggaaggtcgt
     2041 tgatgtttgt cgagccggtg tatttgcagg ctgagcgcag tccgatgccg gagctgcggc
     2101 tggtcgtgct ggctacgcag gagaagctgg gttatgggca aacatttggc gaggcgatga
     2161 atagcttatt cggtgaagcg gccgtgaaac ctccggctga ggaaccagct caaccaacgc
     2221 ctgaacagcc cacaccaact gcggacctac agcagttgat caaccgagca atccaggagt
     2281 ttgaggatta ccagcgactc acctcgcagg gcaaactcgg cgaggccggg cagaagctcg
     2341 agcagcacaa gcgaaccctc gaggagatcc gccagaaaag caaaccttag ccgcggctaa
     2401 atctaactct cggattcgat cttctgtaaa agtttcttcg cgtcttcgac ggcttgtttg
     2461 tgttccgcca cgtatttcgg atcgggagtc gtcgacatca gggtttcgat ctgtttctta
     2521 gcgtcgccat tccgattcac agcttcgtag gctgccgcga gctcgtagcg catcagcgtg
     2581 ttgttgggat tgaacttgac gcccttctcg agatactcga ccgcctttgc cggatcacca
     2641 ccgagcatct tcggcgcctg aagatagagc cgtcctagac cgaggtaagc actatagccc
     2701 tggtaaccct catccagctt caacaccgcc tccatctcgc ccttgatgtc ctggaagctc
     2761 gacaaatttg aaagcgtgct gtgtgcggca ctgccgccgt agtttgcgcc cagccagaaa
     2821 tgcccttcgg g
//
LOCUS       DASSFQ010000192         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1860479, whole genome shotgun sequence.
ACCESSION   DASSFQ010000192 DASSFQ010000000
VERSION     DASSFQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860479"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFH96_06685"
     CDS             complement(<1..904)
                     /locus_tag="VFH96_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973694.1"
                     /translation="MKTKFRNIITRNLAAEKWNFVVAITCTLIVSVADLLRPWPLKLI
                     VDNILLGKTLPPSLRSFAHFFVYQKVWATVAVASVLIVLSAVKGFSVYAQTSITSRIG
                     YRIAHALRRELFSHLQRLSLAFHKRSRSGELLTKVTSDTNSVRDAFSEFALTLVTETL
                     TLVGMILVMFAMNVELSLVVLSTFPILTIISVYRFRRIRDSARKVRKAEGNLAARVSE
                     TLHGIALVKAFGRERYETERFESESSETLNESIRTARLEAASSRAADIVNAVGTFAVL
                     VFGSLQALRGHITPGSLLVFVAYMN"
     gene            complement(894..2096)
                     /locus_tag="VFH96_06690"
     CDS             complement(894..2096)
                     /locus_tag="VFH96_06690"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6973695.1"
                     /translation="MNTTSLLAVDPALPQRDLLLDESLMTEYLSRLIVDIEDCERLRT
                     KYRVGTSLRVLYELNGGGRSHRIAARAFPRTRRVTNQFTSQTEINVPELNATFWIFPH
                     DRKIKNLAVLSNIPDELKNIEGRTWSTSRIVAHAPEKSVTAQCLDDNNQIIAYAKIYA
                     GDEGRQIFQTYKALTGKVQIPRVIAYSETHRVLLLESIAGVPLSTITESENAYFRLGE
                     ALQSLHRITPPSSLPRHTRLTPEAVTRASATISQARPDVADLVQQLSAKLIAQYTHGK
                     PVFLHGDVHPKNIFLNEESLFLLDLDQAATGTATADLGSVIAGLYCDACTGSRTWREA
                     FAQKHALLDGYGAPHDTHELRWHIAAALLQERALRSVTRIRPTALQKLPEILRTAETV
                     LNGGVIEN"
     gene            complement(2093..>3579)
                     /locus_tag="VFH96_06695"
     CDS             complement(2093..>3579)
                     /locus_tag="VFH96_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET6973696.1"
                     /translation="YAWNVFSRKCEFEVEFENQIIAHLAIEVRHLRRNLTAERNHRTL
                     ELYALFIAALALPELDHDGLLEFAVAELHRNLLTDVRPDGVHREQSTHYHMTALRSFL
                     GARENALRFGVEFPAGYDERLEKACEFLLHVQRPDGLIPALSDADTGSYSDLLALAAT
                     IFRREDFLCAASAETCPSFPDGGYFIQRSGWDDRARYLVFDCGPIGDGGHGHYDLLSI
                     EVAANGRPLIVDPGRFTYAESDWRRQFKGTAAHNTVCVDDLDQVEYRRGKPKPPLAQG
                     RLLERFNGHGLDMLCGEVRSPMYEAVHTRRIFFVAGEYWLLVDSLRGSVSHKYDLRFH
                     LTAAALNHCTRIAGGIRTPDMVLLLSNCRGGPPWPPVAQPPRGYEERAATEGRPYSCS
                     LSHGWISPRYGIKQAAPVVSFVSTGEANADFYTLIAPRTLSAPLPQLQVLSNSDPLYS
                     GRVRIEGVGENFTEVDELSWHRDSSSGVTGPTWTGQVAWRRVSL"
BASE COUNT          778 a          956 c         1060 g          785 t
ORIGIN      
        1 tgttcatgta cgcgacgaac acgagcagcg atccgggtgt gatgtgcccg cgcaaagcct
       61 ggagcgagcc gaatacgagg accgcaaacg ttcccactgc gttgacgatg tctgcggcgc
      121 gcgatgatgc ggcttcgaga cgagccgtgc gtatgctttc gttcagcgtc tccgagcttt
      181 ctgactcgaa ccgttccgtc tcgtaacgtt cgcgaccgaa agctttcacg agcgcgatcc
      241 cgtgtaacgt ctcgctgacg cgcgcggcca gattgccttc ggctttgcgg acctttcgcg
      301 ccgagtcgcg tatgcgccgg aagcgataga cagaaatgat tgtcagaata ggaaatgttg
      361 acagcaccac gaggctcagc tcgacgttca tcgcaaacat cacgaggatc attccgacga
      421 gcgtgagcgt ttcggtgacc agtgtcaggg cgaattctga aaacgcatcg cggacggagt
      481 tggtgtcgct ggtgactttc gtcagcaact cgccggatcg agatcgtttg tgaaatgcga
      541 gcgagaggcg ttgtagatgt gaaaacagct cgcggcgtag tgcgtgcgcg attcgatatc
      601 cgatgcgtga cgtgatcgat gtttgcgcgt aaacggagaa gcctttgacg gcgctcaata
      661 caatcagcac gctcgcgacc gcgactgtgg cccatacctt ttgatagaca aagaagtgtg
      721 caaacgagcg cagtgaaggc ggcaaagttt tgccgaggag aatgttgtcg acgatcagtt
      781 tcaacggcca ggggcgtagt aaatccgcaa ctgaaacgat gagcgtgcag gtgatggcga
      841 cgacgaagtt ccatttctcg gcggcgagat tgcgagtgat gatgtttcgg aacttagttt
      901 tcaatgacgc ctccgttcag cacggtctcg gctgtgcgca ggatctccgg cagcttttgc
      961 agtgcggtcg ggcggatgcg tgtgacggag cgtagagcgc gttcctggag taacgctgcg
     1021 gcgatgtgcc agcgcagttc atgtgtgtcg tgcggcgcgc cgtagccgtc tagtaaggca
     1081 tgtttttgag cgaacgcttc acgccaggtg cgcgagccgg tgcaggcgtc gcagtaaaga
     1141 ccggcgatca cgctgccgag atctgctgtc gcagtgccag tcgccgcctg gtccaggtcg
     1201 agcaggaaca agctctcctc gttcagaaag atgttttttg gatgaacgtc gccgtgtaag
     1261 aacacgggtt tgccgtgcgt gtattgcgcg attagtttgg cgctcagttg ttggaccaga
     1321 tctgcaacat caggtctggc ctggctgatg gtcgcgctgg ctcgcgttac tgcttccggt
     1381 gtcagtcgag tatggcgcgg aagcgatgaa ggcggggtga tgcgatgaag actctgcaac
     1441 gcttcgccta gccggaagta tgcgttctcg ctttcagtga tagtagacaa cggaacaccg
     1501 gcaatggatt caagcagcag cacacgatgt gtttcggaat atgcgattac tcgcgggatt
     1561 tgtacctttc ctgttaaggc tttgtatgtt tggaagatct ggcggccttc atcacctgca
     1621 tagatcttcg cgtaggcgat gatttggttg ttgtcgtcga ggcactgtgc ggttactgat
     1681 ttctccggcg cgtgggcgac gatgcgactc gtggaccatg ttcgtccttc gatgtttttc
     1741 agttcgtccg gaatattgct tagcaccgcg aggtttttga tctttcgatc gtgcggaaag
     1801 atccagaagg tggcgtttag ttcgggaacg ttgatctctg tctgcgatgt gaattgatta
     1861 gtaacccggc gtgttcgtgg gaaagcgcga gccgcgatgc gatgtgaccg accgccgccg
     1921 tttaactcgt aaagcacccg caggctcgta ccgacgcgat actttgtccg gaggcgctcg
     1981 cagtcttcga tgtcaacgat taggcgagac aagtactccg tcatcaggct ctcatcgagt
     2041 aacagatcgc gttgcggcag agccggatcg acggccagta atgaagtcgt gttcataacg
     2101 aaacgcgcct ccacgccact tgccctgtcc aagttggacc agtgactcct gatgaagagt
     2161 ccctatgcca actcagttcg tctacttcgg tgaagttctc gccaacgcct tcgatgcgaa
     2221 cacgacctga gtagagcgga tccgaattgc tcaggacttg aagctgtggc aatggcgcgc
     2281 tcaacgtacg cggcgcgatc aatgtgtaga aatctgcgtt tgcttctccg gtcgaaacaa
     2341 aactgaccac cggcgccgct tgtttaatgc cgtagcgcgg tgagatccat ccatgactta
     2401 aagaacaact gtaggggcgg ccctctgtgg ccgcccgttc ttcgtaaccg cgcggtggtt
     2461 gagcaacggg tggccacgga gggccacccc tacagttgct caatagaagc accatgtcgg
     2521 gcgtgcggat gccgccggcg atgcgggtgc agtgattcag ggctgcggcg gttaaatgga
     2581 aacgaagatc gtacttgtga gaaacgctgc ctctaagact gtcgacgagc agccaatact
     2641 cacctgcgac aaagaatatt cgtctcgtgt gaaccgcttc gtacatcgga ctgcgaacct
     2701 caccgcagag catgtcgagg ccgtgcccgt tgaagcgttc gagtaatcga ccctgtgcga
     2761 ggggaggctt cggcttgccg cggcggtatt caacctggtc cagatcatca acacagaccg
     2821 tgttatgtgc cgccgttccc ttgaactgac gacgccaatc cgattcggca tacgtgaaac
     2881 gtcccggatc gacaatcagc ggacgcccat ttgccgcgac ctcaatactc agcagatcgt
     2941 agtgaccatg accaccatct ccgatcggac cacagtcgaa gacgagatat ctcgcgcgat
     3001 cgtcccaccc gcttcgctga atgaaataac caccgtcagg aaagctcggg cacgtttctg
     3061 ccgaggcggc gcagagaaaa tcctcgcgtc tgaaaatcgt tgccgcgagc gccaacagat
     3121 ccgagtaact acccgtatct gcatccgaca gcgcgggtat caaaccgtcc ggccgctgaa
     3181 catgaagcag aaactcacaa gccttttcga gacgctcgtc gtacccggca ggaaactcaa
     3241 ctccaaagcg aagggcgttc tcgcgtgcgc ccagaaacga acgcaacgcc gtcatgtgat
     3301 agtgcgtcga ctgctcgcga tgtacgccgt ccggtcgcac atctgtgagc agattgcgat
     3361 gcagctccgc aaccgcaaac tcgagcaaac cgtcgtggtc cagctcaggc agtgccaaag
     3421 ctgcgatgaa cagtgcgtag agctcgagcg tgcgatgatt gcgttcggcg gtcaggttgc
     3481 ggcgtaagtg acggacttca atggcgagat gggcaataat ctgattctca aactcaactt
     3541 caaactcaca tttccgtgaa aaaacattcc acgcgtaaa
//
LOCUS       DASSFQ010000193         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1575317, whole genome shotgun sequence.
ACCESSION   DASSFQ010000193 DASSFQ010000000
VERSION     DASSFQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575317"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="trpA"
                     /locus_tag="VFH96_06700"
     CDS             complement(<1..587)
                     /gene="trpA"
                     /locus_tag="VFH96_06700"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET6973697.1"
                     /translation="MGRIADAFTTLKRDRKKGFIPYITAGDPDLATTEQLLVTLAQHA
                     TLIELGVPFSDPMADGPVIQRASERALKNNFGLQDILDTAARARKQIETPIILFSYYN
                     PLLQFGLKRVAGAASEAGIDGVLVTDLTPEESGAFEAELRAHDLDMIFLIAPTSTDER
                     LKLVAKHASGFIYAVSRAGVTGTRETVSTEAEKLV"
     gene            complement(591..1796)
                     /gene="trpB"
                     /locus_tag="VFH96_06705"
     CDS             complement(591..1796)
                     /gene="trpB"
                     /locus_tag="VFH96_06705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET6973698.1"
                     /translation="MPTAITQPDSLGHWGQYGGRFVPETLMAPLEELTTAYLASCDDE
                     SFQSELKDLLKNYAGRPTPLFHAARLTEHCGGATIYLKREDLLHTGAHKINNTIGQVL
                     LARRMNKKRIVAETGAGQHGVATATVCALFGLDCVVYMGAEDMRRQALNVFRMQLLGA
                     DVREVNAGSRTLKDAINEALRDWVTNVGDTYYLLGSALGPHPYPLMVRDYQSVIGREA
                     RKQILEQEGRLPDVLIACVGGGSNSIGLFHPFLADTHIRMIGVEAGGSGNELGQHAAR
                     FNVAGGGRPGVLQGTMSYVLQDTNGQVAATHSISAGLDYPSIGPEHAFLHDAGRVEYV
                     SVSDSEALEAFQLLSKLEGIIPALESAHAVAHALKTAREMKSDQILVVNLSGRGDKDI
                     NSVREALES"
     gene            complement(1873..>2464)
                     /locus_tag="VFH96_06710"
     CDS             complement(1873..>2464)
                     /locus_tag="VFH96_06710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6973699.1"
                     /translation="PLEVDLLVERTQQLVLLRQKIDRLRADADLDSLTGLANRRRFRV
                     ALAREVERWRRYRVPCALLMLDIDHLKFVNDHYGHPVGDSVIRQIAQTLKEVSRDNDT
                     AARLGGEEFALLLAGVDLDRAVAAAERLRTVLSNKRVEGVGTVTVSIGVAGCPESAAS
                     ERALYSASDEALYVSKNAGRDRVSVAPPLQEKLPGV"
BASE COUNT          526 a          766 c          646 g          526 t
ORIGIN      
        1 ttcacaagct tctccgcttc cgtgctcacc gtctcacgcg tgcccgtcac tccggctcgc
       61 gacacagcgt agatgaatcc actcgcatgc ttcgccacca gcttcaagcg ctcgtcagtc
      121 gacgtcggcg cgatcagaaa aatcatatcg agatcgtgcg cgcgcaactc cgcttcaaac
      181 gcgcctgact cttccggtgt cagatcagtc accaacacgc catcgatccc cgcctcactt
      241 gccgcgccag ccactcgctt caaaccaaac tgaagcagcg gattgtaata actgaaaaga
      301 atgatcggcg tctcaatctg ctttcgcgcc cgcgccgcag tgtcgagaat atcctgcaag
      361 ccgaaattgt ttttgagagc cctttccgaa gcgcgttgaa tcactggtcc atcagccatc
      421 ggatcgctga acggtacacc gagctcgatc aacgttgcgt gctgtgcgag agtgacgagg
      481 agttgttcgg tggttgcgag gtcgggatcg ccggcggtga tgtagggaat gaatcccttc
      541 ttccggtctc gcttcagagt tgtgaaggcg tcagcgatgc gtcccaacgg ttacgactcc
      601 aatgcttcgc gcacggaatt tatatcttta tctccgcgtc cggaaaggtt cacgacaagt
      661 atttgatcgc tcttcatctc gcgcgcggtt ttgagtgcat gggccactgc gtgcgctgac
      721 tcgagcgcgg gaatgatgcc ttcgagttta gacagaagct gaaacgcctc gagcgcctcg
      781 ctgtcagaaa cggaaacata ctcgacgcgt cccgcatcgt gcagaaacgc gtgttccgga
      841 ccgattgaag gatagtcgag accggccgag atcgagtgag tcgctgctac ctggccgttc
      901 gtgtcctgca agacgtaact catcgtgccc tgcaaaacgc cgggacgtcc gccacccgcg
      961 acgttgaatc gcgccgcgtg ttggcccagc tcgttgcctg agccaccggc ttcgacacca
     1021 atcatcctga tatgcgtgtc ggcgaggaat ggatgaaaca gtccgatgga attcgagccg
     1081 ccaccgacac acgcgatgag aacgtccggc agccggcctt cctgttcgag aatctgctta
     1141 cgtgcttcgc gaccgatcac gctctgatag tcgcgcacca tgagtgggta tggatgtggg
     1201 ccaagcgcgc tgccgagtaa ataataggta tcgccgacgt tcgtcaccca atcgcgtagc
     1261 gcttcgttga tcgcgtcttt gagcgtgcgc gagccggcat ttacctcgcg aacgtcagcg
     1321 ccaagcagtt gcatgcgaaa gacgttcaac gcctggcgac gcatgtcttc ggcgcccatg
     1381 tagacaacgc agtcgagccc gaagagcgcg cagactgttg ccgtcgcgac accatgctgg
     1441 ccagctccgg tctcagcaac gatgcgtttc ttgttcatgc gccgcgcgag cagcacttgc
     1501 ccgatcgtgt tgttgatctt gtgtgcgccg gtgtgcagga gatcttcgcg tttgagatag
     1561 atcgtagcgc ccccgcagtg ttcggtaagt cgcgcggcgt gaaagagagg cgtgggtcga
     1621 cccgcgtagt ttttcagcag gtctttcagt tctgattgaa acgattcatc gtcacagctc
     1681 gcgaggtacg ccgtggttag ttcttcgagc ggtgccatca acgtctcagg tacaaagcga
     1741 ccaccgtact gtccccagtg accaagactg tcaggctgtg tgattgctgt tggcatactt
     1801 tacaagatta caagatagaa caggatttac atgttaatcc tgaaaatctt gtaaatcatg
     1861 tctacagttt cgttagactc cgggcagttt ttcctgcaac ggcggcgcca ccgacacgcg
     1921 atctcgcccc gcattcttcg acacatacag cgcctcatcg ctcgccgagt aaagcgctct
     1981 ttcagacgcc gcactctccg gacagcccgc caccccaata ctcaccgtga ccgtcccaac
     2041 accttccaca cgtttattac tcagcaccgt gcgcaggcgc tcagccgccg ccaccgcgcg
     2101 gtccagatcg acaccggcca gcaacagcgc aaactcttca ccaccgagtc gtgccgccgt
     2161 gtcgttgtct cgcgaaacct ctttcaacgt ctgcgcgatc tggcgaatca cactatcgcc
     2221 aacgggatgc ccataatgat cattcacgaa cttcaagtga tcgatatcga gcatcaacaa
     2281 cgcgcacgga acacgataac gccgccagcg ctcaacctct cgcgccaacg ccacccgaaa
     2341 ccggcgacga ttcgccagcc cagtcaatga atccaaatct gcatccgctc gcagtcgatc
     2401 gatcttctga cgcagcaaga ctaactgctg cgttcgctcg accagcagat caacttcaag
     2461 cggc
//
LOCUS       DASSFQ010000194         8118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1120352, whole genome shotgun sequence.
ACCESSION   DASSFQ010000194 DASSFQ010000000
VERSION     DASSFQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120352"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="VFH96_06715"
     CDS             complement(<1..1531)
                     /locus_tag="VFH96_06715"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HET6973700.1"
                     /translation="MPIAPSYPGVYIEEIPSGVRTITGVATSITAFIGRAERGPVNEP
                     TTITSFGDYERTFGGLNLLSTMSYAVRDFYQNGGSQAIIVRVIRNGSTARFSLPVEAG
                     SPPATSPPSGNLIIEAASAGAWGRNLTVIVDYNTADPTAAASPPPPVNPELFNLIVRE
                     TVGTTIRQEEKFLLVSMNPSSPRFLPRVLEKNSLLLRVPKVNGVFQFPVDGDGDGLRP
                     LVTGTPATPTQGSDGVDITATELTGSANDKTGIFALDKADLFNLLCIPPAVRDGDVDA
                     SVLQAAAAYCTKRRAMLIVDPPIDWGRRAETAAASARAGLGDLNLSGTIARNAALYFP
                     RVVQADPLRDRQNDVFVPCGIIAGVMARTDVQRGVWKAPAGQDAALNGIQGLQVNLTD
                     DENGLLNPLGINNLRSFPVIGRVVWGARTLDGADRLASEWKYIPVRRTALFIEESLYR
                     GTQWVVFEPNDEPLWAQIRLNVGAFMQNLFRQGAFQGRTPREAYFVKCDKETTTQNDI
                     NL"
     gene            complement(1574..1858)
                     /locus_tag="VFH96_06720"
     CDS             complement(1574..1858)
                     /locus_tag="VFH96_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973701.1"
                     /translation="MERLNSSVDLPRDDLIVRILRYLVNHPTAKDTTKGIEKWWLSGA
                     SRGGTSTEDALKLLVTKGWLTARYSPQSETLYSLNESYLKEIMAFLDAEP"
     gene            complement(2018..3391)
                     /locus_tag="VFH96_06725"
     CDS             complement(2018..3391)
                     /locus_tag="VFH96_06725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6973702.1"
                     /translation="MGNALIVNGSHFTPCAHGCASLLTLLQRTNFFDRVQERPFESPP
                     RDSQLTHDLILPRFTLKDWTKESVRFWKRTWASAKTFAVLCDQTLKHDWQIPPALIDV
                     DDFLVCPYKENELLLRLKRLLKTQRTATTKQNGESPRDLDLLVGESETFMRVLRKVSL
                     LAQSKAPVVIAGETGSGKELLARAIHYQSVRKSKPFIPVNCGALPDELFENELFGHLK
                     GAYTHASSNEKGLIAEAEGGTLLLDEVDALSPAAQIKLLRFLQSGEYRPLGSSRSVVA
                     DVRIIAATNTDLHTQVELKEFREDLHYRLNVLSLSIPPLRDRGRDVLSLASHFLAVYS
                     KEHQRFAPQLSPDALEKLLNYGWPGNVRELEGVIQRAIVLQPAPVLTPDDIELPKAND
                     RCLLEPESFRLEKSLAIGQFERDLVARLLEQHEGNISRAAKSVGKDRRSFQRLVNKYG
                     FDRRTFK"
     gene            complement(3470..5029)
                     /locus_tag="VFH96_06730"
     CDS             complement(3470..5029)
                     /locus_tag="VFH96_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET6973703.1"
                     /translation="MHERVLPVLRAPLERVHDWNEFHTHLNGSTLREQGGRCLDCGIP
                     FCHTGELINGAASGCPLNNLIPEWNNLVYRGNWRAALDLLHQTNNFPEFTGRVCPAPC
                     EGSCVRALTDEPVTIKTIECAIVDRGFDEGWIVPRPPQRRTGKRVAVIGSGPAGLACA
                     AQLNSSGHSVKVFEKADRVGGLLMYGIPNMKLDKAIVQRRVDLLAAEGVDFVTNTNIG
                     RDYPVERLRDEFDAVVLCGGASRPRDLAVENRNLPGVHFAMEFLRANTRNLLDGSDFI
                     SAKDKDVIVIGGGDTGTDCVATALRHGCRSLVQFEIMQQSPLARAADNPWPQWPRIHR
                     VDYGHEEAIALRGNDPRMYSVMTRRFIGEGAVEAVETVEVDSTFSEISDTGRVWPAQL
                     VLLAMGFLGPEKDGLISQLGVTLNTRGLVAVDANKMTNIPGVFAAGDIERGQSLVVWA
                     IADGRHAATCVDKYLTLSTDYADEIKRICVICGWSYFLLTQSTRARILPHLSQLEEFL
                     RTLLSAVRSPT"
     gene            complement(5022..>8118)
                     /gene="gltB"
                     /locus_tag="VFH96_06735"
     CDS             complement(5022..>8118)
                     /gene="gltB"
                     /locus_tag="VFH96_06735"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HET6973704.1"
                     /translation="RVFGYTHEELRLILEPMAASGSEAIGSMGTDTPLAILSQRPQLL
                     YNYFKQLFAQVTNPPVDAIREELVMSADTCVGPEVNMLEPSPECARQIRLSSPILTNE
                     ELEKLRLFGDSDSHWGRSGIKSLTIPIVYLVVGGGNGLASALDNICEQSIKAIDGGYD
                     LIILSDRNIDVEMAPIPALLAVGAVHHHLIREGTRTHVGLIVESGEPREVHHFALLLG
                     YGAAAINPYLVFETLHDRIRQGLLQETDEPTALHNYVHAINKGIIKVISKMGISTTQG
                     YCGAQIFEAIGLDHDLVDKYFTWTPSRIGGANLEVVAEEIRRRHAAAFVEEKPALESG
                     GHYQYRKDGEPHLFNPLTVHKLQAACRTGNYTTFKEYSELVNNQSRNLCTLRGVMEFR
                     SNRPPINIAEVEPPEAIVKRFKTGAMSYGSISKEAHESIAIAMNRIGGKSNTGEGGED
                     PERFADQRNSAIKQVASGRFGVTSHYLVNAQELQIKIAQGAKPGEGGQLPGEKVYPWI
                     ATVRHSTPGVGLISPPPHHDIYSIEDLAQLIYDLKNANPEARISVKLVAESGVGTIAA
                     GVAKARADVVLISGYDGGTGAAPLTSIKHAGAPWELGLAETHQTLLLNNLRSRITIET
                     DGQLKTGRDVVIAAMLGAEEFGFATAPLVTLGCIMMRACHLNTCPVGVATQDPKLRAK
                     FTGDPSHLVNFMMFIAQEVRELMAQLGFRNVNEMVGRSECLEMENVEHWKARHLDLSA
                     VLFKPDVPASVGRYCQTKQRHDLENTLDSKLLLDLCRPALRDGEPVSATLPIRNVHRA
                     VGTRLGSEVTRQYGPAGLPHDTIQLRFEGSAGQSFGAFIPKGITLRLEGDANDYLGKG
                     LSGGKIVVVPPKNATFVPEENVIIGNVVFYGATSGEAYINGIAGARFAVRNSGANVVV
                     EAVGDHGCEYMTGGRVVVLGQVGRNFAAGMSGGIAYLVDDNNNQGKFCNLEMVSLTSL
                     DDQAEIEIVKDMIFRHAEQTGSRRATEIILAWDQFLSKIIRVMPNDYARALEKVALAH
                     A"
BASE COUNT         1739 a         2301 c         2251 g         1827 t
ORIGIN      
        1 ctagattgat gtcgttttgc gtggtggtct ctttgtcgca cttgacgaag taggcttcgc
       61 gcggcgtgcg cccctgaaag gcgccctgtc tgaagaggtt ctgcatgaag gcgccgacgt
      121 tgagccggat ctgcgcccag agcggctcgt cgttcggctc gaagaccacc cactgcgtgc
      181 cgcgataaag gctctcttcg atgaagagcg ccgtgcgccg caccgggatg tatttccact
      241 ctgaggccag gcggtcggcg ccgtccagag tgcgcgctcc ccaaacgact ctgccgataa
      301 ctgggaaact gcgcaggtta ttgatcccga gtggattcaa cagtccgttc tcatcgtcgg
      361 tgagatttac ttgcaggccc tgaattccat tcagcgccgc gtcctgaccg gcgggcgctt
      421 tccagacgcc gcgttgcacg tcagtgcgcg ccatcacgcc ggcgatgatt ccacacggaa
      481 cgaagacgtc gttctgtcga tcgcgtaacg gatctgcctg gaccacacgc gggaaataaa
      541 gcgcggcgtt gcgagcgatg gtgccggaaa gattgagatc gccgagtccg gcgcgcgcac
      601 tcgcggcggc ggtttcagca cgacggcccc aatcgatcgg cggatcgacg atcaacatcg
      661 cgcgccgttt ggtgcagtaa gctgccgccg cttgaaggac cgacgcatcg acgtcgccat
      721 cgcggacggc aggtggaatg cacaacaggt tgaagagatc ggctttgtcg agcgcgaaga
      781 ttccggtctt gtcgttggca ctgccagtga gctcagtcgc ggtgatgtcg acgccgtcgc
      841 tgccctgagt tggagttgcc ggagttccag ttacgagtgg ccgcaagcca tcgccgtcgc
      901 cgtcaactgg aaactggaaa actccgttga ctttcggaac gcgcaagagc agcgaattct
      961 tctccagcac gcgcggcagg aagcgaggtg agctgggatt catcgaaacg agcaggaatt
     1021 tttcttcctg ccggatcgtc gtgccgactg tttccctgac gatgagattg aacagctccg
     1081 gattcactgg cggtggcggc gaagcggcgg cagtcggatc ggcggtgttg tagtcgacaa
     1141 tcaccgtcag attgcgtccc cacgcgcctg cacttgcagc ctcgattatc aggttaccgc
     1201 tcggcggcga agtcgcgggc gggctgcccg cttccaccgg caggctaaaa cgcgcagtgc
     1261 tgccgtttct gatgacgcgc acgatgatgg cctgactgcc gccattctga tagaagtcgc
     1321 ggacggcgta actcatcgtg ctgagcaaat tcaatccgcc aaaggtgcgc tcataatcac
     1381 caaaactggt gatcgtcgtc ggctcattca ctggaccacg ttcggcgcgt ccaatgaacg
     1441 cggtgatcga tgtggcaact ccagtgatcg tgcgcacgcc actcggaatc tcttcaatgt
     1501 atacacccgg ataagaaggt gctattggca ttatcggtcc ttcctttcag ttaacagcgg
     1561 cagggatcca taactagggt tctgcgtcga gaaacgccat gatttctttt aggtagcttt
     1621 cgttaagcga ataaagtgtt tctgattgag gtgagtagcg ggcggttagc cagcctttgg
     1681 tcaccagtaa tttgagagcg tcttcggttg atgttcctcc acgactggcg ccggacagcc
     1741 accatttctc gatgcccttg gtcgtatctt tcgccgtcgg atgattgacc aggtagcgca
     1801 gtatgcgaac gatcaagtca tcgcgcggca gatcaactga tgaattgaga cgttccattg
     1861 cacaaccatt acgggttgag ccatggcaaa gaggaggcca aaaggttttg ttgagtattt
     1921 aagaagtgag cgactaaaag tgaggctttg ctaccgcact aagtgcggct gaatccccgc
     1981 attaaaatcc gtgcttttta tccgcggtga tccgtggtta cttaaacgtc cggcgatcaa
     2041 atccgtactt gttaaccagt cgctgaaatg aacgccgatc ttttccgact gacttcgctg
     2101 cgcgcgaaat atttccttca tgttgctcca gcaatcgggc caccaagtcg cgttcaaact
     2161 gtccgatcgc aagcgatttc tccagacgaa atgactccgg ctcgagcaga cagcgatcgt
     2221 tcgctttcgg tagttcgatg tcatcgggtg tgagtaccgg cgccggctgc aacacgatgg
     2281 cgcgttgaat aacgccttcc agctcgcgca catttcccgg ccaaccgtaa ttcagcagtt
     2341 tctccaatgc gtccggcgat agttgtggcg caaaacgctg gtgttccttt gagtagacgg
     2401 cgaggaaatg cgacgcgagg ctcaaaacgt cgcgaccacg atcgcgcagc ggtggaatcg
     2461 aaagcgaaag cacgttgagc cggtagtgaa ggtcttcgcg aaattccttg agttcaactt
     2521 gcgtgtgtag atcggtattc gtcgccgcga taatgcgcac gtccgccacc accgagcgtg
     2581 acgatccgag tggccgatat tcaccactct gcaaaaatcg cagcaatttg atctgagcgg
     2641 caggcgacag agcgtctact tcgtcgagta gtagagttcc gccttcggct tcggcgatta
     2701 atcctttttc gttggaagac gcgtgtgtgt aagcgccttt gagatggcca aagagctcgt
     2761 tttcgaagag ttcatccggc agtgcgccgc agtttacggg aatgaaaggc ttcgatttcc
     2821 tgacgctttg ataatgaatt gcgcgcgcga gcaattcctt gccgcttccc gtttcgccgg
     2881 cgatgacgac cggcgctttc gactgtgcca gcaggctcac tttgcgaagc acgcgcataa
     2941 atgtctcact ctctccgacg agcaaatcga gatcgcgcgg gctctcgcca ttctgttttg
     3001 tagttgctgt gcgctgcgtc tttaacagac gtttcagccg caataacagc tcgttttcct
     3061 tgtatgggca gacgaggaaa tcgtctacat cgatgagcgc cggcggaatc tgccaatcgt
     3121 gtttgagagt ttgatcgcac aaaacggcga aggttttggc tgacgcccag gtgcgtttcc
     3181 agaaccggac gctttctttg gtccagtctt tgagggtgaa acgcggcagg atcaggtcgt
     3241 gagtgagttg cgaatcacgc ggtggagatt caaacggtct ttcctgaacg cgatcgaaaa
     3301 aattggtgcg ttgcagcagg gtcaggagcg aggcacaccc atgcgcgcaa ggcgtaaagt
     3361 gacttccatt gacaataagt gcgtttccca cggcgcttgc aatccaggct cttacgaggt
     3421 gtgacggtta acgacgggcg ttgggggtac gcgaaaacac agtctggcct caggtcggac
     3481 ttcgaacggc agaaaggaga gtgcgaagga actcctcaag ctgcgatagg tgcggcagta
     3541 tacgcgcccg agtcgattgg gtcaataaaa agtaggacca tccacagatt acacagattc
     3601 tcttaatttc atctgcgtaa tctgtggata aagttagata tttgtccacg caggtggcgg
     3661 cgtggcggcc gtcggcgatt gcccagacga cgagcgattg gccacgttcg atgtcgccgg
     3721 cggcgaagac accgggaatg tttgtcattt tgtttgcatc aacggcgacg aggcctcgcg
     3781 tgttcaacgt cacgccgagt tgagaaatca atccgtcttt ttcgggtccg agaaatccca
     3841 tcgcgagcag caccagctgc gcaggccaca cacgaccggt gtctgaaatc tcgctgaagg
     3901 ttgaatcgac ctcgacagtc tccactgcct cgaccgcacc ctcaccaatg aagcgccgcg
     3961 tcatcaccga atacatgcgc ggatcattgc cgcgcaacgc aatcgcctct tcatgcccgt
     4021 aatcaacgcg atggatccgc ggccactgcg gccaaggatt atcggcggcg cgagcgagcg
     4081 gcgactgctg catgatctca aactgaacca gactccggca gccatgtcgt aacgctgtcg
     4141 cgacgcagtc agtgccggta tctccgcctc cgataacaat cacgtcttta tctttcgcgg
     4201 agatgaaatc ggatccatcc agcaggttcc tggtattcgc gcgcagaaat tccatcgcga
     4261 aatgaacacc gggcagattt ctattctcaa ccgcaaggtc gcgcggacgg ctggcgccac
     4321 cacacagcac gacagcatcg aactcatccc gcaatcgctc gaccggataa tcgcgaccaa
     4381 tattagtatt ggtaacgaag tcgacacctt cggcagcaag caaatccacg cgtcgttgaa
     4441 cgatcgcttt atcgagtttc atgtttggaa tgccgtacat caacaatcca ccaacacgat
     4501 cggccttctc aaacactttt accgaatgcc cgctggagtt gagttgagct gcgcaagcca
     4561 ggcctgctgg tccggatcca atgacggcaa cacgctttcc ggttcgacgc tgaggcggac
     4621 gtggaacaat ccaaccctca tcgaatccgc gatcgacgat cgcgcattca atcgttttga
     4681 tcgtcactgg ctcatcggta agtgcacgca cgcacgaacc ctcgcatggc gccggacaaa
     4741 ctcggcccgt gaactccgga aagttattcg tctggtgcag cagatcgagc gccgcacgcc
     4801 aattgcctcg atagacgaga ttgttccact cgggaatcag gttgttgagc gggcaaccag
     4861 aagcggctcc attgatcagc tctccggtgt gacagaacgg tatgccgcaa tccaggcagc
     4921 gtccgccctg ttcgcgcaga gtcgagccgt taagatgcgt gtgaaactcg ttccaatcgt
     4981 gaacgcgttc caaaggtgct cgcagcacgg gaagaacgcg ttcatgcatg ggccaaggcc
     5041 accttctcta acgcgcgggc gtaatcattc ggcatcacgc ggattatttt cgaaagaaac
     5101 tgatcccagg cgaggataat ctccgtggcg cgccggctgc cggtttgctc cgcatgacga
     5161 aagatcatgt ctttaacaat ttcaatctcc gcctgatcgt cgagtgacgt caacgaaacc
     5221 atctcgagat tgcagaactt cccctggttg ttgttgtcgt caacaagata agcaatgcct
     5281 cccgacattc cggcggcaaa gtttcttccg acctggccca acacgacgac tcgtccacct
     5341 gtcatgtact cgcagccatg atcgccaacc gcttcaacca ccacattcgc accgctgtta
     5401 cgaaccgcaa agcgcgctcc cgcaatgccg ttgatgtaag cttcgccact cgtcgcgcca
     5461 taaaacacga cgttgccgat gatgacgttc tcttccggaa caaacgtcgc attcttcggc
     5521 ggcacgacga cgatctttcc acctgacaaa cccttgccga gatagtcgtt ggcatctcct
     5581 tcgagtcgga gcgtgatgcc tttcggaata aacgcgccaa aactctggcc cgcagatcct
     5641 tcgaagcgaa gctgaatcgt gtcgtgcggc aaaccggctg ggccatactg gcgcgtcact
     5701 tcactgccga gtcttgttcc aactgcacga tgaacgttgc gaatgggtag tgttgccgac
     5761 actggctcgc cgtcgcgcaa cgcaggccgg caaagatcga gtagcaactt gctgtcgagc
     5821 gtgttttcga gatcgtgacg ctgttttgtc tggcagtagc gaccaacact cgccggaaca
     5881 tctggcttga agagcaccgc cgacagatcg agatgccgcg ctttccagtg ctcgacgttc
     5941 tccatttcca aacactccga acgtcccacc atctcgttca cgttccgaaa gccgagttgg
     6001 gccatcagtt cgcgcacttc ctgggcgatg aacatcatga agttaacgag gtgtgaagga
     6061 tcgccggtga acttcgctcg cagtttcgga tcctgcgtcg cgacgccgac aggacaggta
     6121 ttgagatgac acgcacgcat catgatgcaa ccgagcgtca cgagtggcgc agttgcaaag
     6181 ccaaactctt ccgcgccgag cattgcggcg ataactacgt cacgacctgt cttcaactgc
     6241 ccgtcagttt cgatcgtgat gcgactgcga agattgttca gcaacagtgt ttggtgtgtc
     6301 tcggcgaggc caagctccca gggcgcgccc gcgtgcttga tgctcgtgag cggtgcggcg
     6361 cctgtgccgc catcgtaacc gctaatcaga actacatctg cgcgagcctt cgcgacaccg
     6421 gctgcaatcg tgccgacacc cgattccgcc accagcttca cactaatgcg cgcttcggga
     6481 ttcgcgttct tcaggtcgta aatgagttgg gccagatctt cgatcgaata gatgtcgtga
     6541 tgcggtggtg gcgagatcag tccgacgccc ggtgtcgagt gtctgacggt cgcgatccat
     6601 ggataaactt tttctccggg aagttgacca ccttcacctg gctttgcgcc ctgtgcgatc
     6661 ttgatctgca gctcctgcgc gttgacgagg taatgactcg tcacgccaaa ccttccagac
     6721 gcaacctgtt tgatcgcgct gttccgttga tcggcgaagc gttccggatc ctcgccaccc
     6781 tcgcccgtgt tgctcttgcc gccgatgcgg ttcatcgcaa tcgcgatcga ctcgtgcgct
     6841 tctttgctga tcgaaccgta tgacatcgcg cctgttttga atcgcttgac gatcgcttcg
     6901 ggaggctcga cttcggcaat attgatcgga ggacgatttg atctgaactc catcacgcca
     6961 cgcaaggtgc aaagattgcg cgactgattg ttgactagct cgctgtactc tttgaacgtc
     7021 gtgtaatttc cggtccgaca cgctgcttgt agcttgtgaa cggtcagagg attgaagaga
     7081 tgtggctcac cgtctttgcg atactgatag tggccaccgc tttctaatgc cggtttttct
     7141 tcgacgaacg ctgccgcgtg ccggcgtcga atttcttctg ctaccacttc gagattcgcg
     7201 ccaccaatcc ttgaaggtgt ccaggtgaag tatttatcga cgagatcgtg gtccagtccg
     7261 atagcttcga agatctgagc accacagtat ccctgcgtcg ttgagatgcc catcttcgag
     7321 atgaccttga tgatgccttt gttgatagcg tgcacataat tgtgcagcgc cgtcggctcg
     7381 tcggtttcct gaagcagccc ctgacgaatg cggtcatgaa gggtttcaaa aacgaggtat
     7441 ggattgatcg cggcagcacc gtaacccaac agtaaagcaa agtgatgcac ttcacgcggc
     7501 tcgccactct cgacgatcaa accgacgtgc gtgcgggtgc cttcacgaat caggtgatga
     7561 tgcaccgcgc caactgcaag caacgccggt atcggcgcca tctcaacgtc gatgttgcgg
     7621 tcggagagaa tgatgagatc gtatccgccg tcgatcgctt taatcgactg ctcgcaaatg
     7681 ttgtcgagcg cgcttgcgag tccattcccg ccgccaacaa ccaaataaac tattggaatc
     7741 gtgagcgatt tgattccgga tctgccccag tgactatcag agtcgccaaa gagtcttagc
     7801 ttctccagtt cttcgttagt cagaatcggc gatgagagtc tgatctgacg cgcgcactcg
     7861 ggcgacggct cgagcatgtt cacttccggt ccaacgcacg tatcggcaga catcaccagc
     7921 tcctcgcgaa tcgcatcgac gggcggattc gtcacttgcg cgaagagttg tttgaagtag
     7981 ttgtagagca actgtgggcg ctgcgacaga atcgcgagcg gcgtgtcagt gcccatcgag
     8041 ccgattgcct cgctcccgct cgcagccatc ggttcgagta ttaatcgcag ctcttcgtgc
     8101 gtgtaaccaa aaacacgt
//
LOCUS       DASSFQ010000195         5106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2173076, whole genome shotgun sequence.
ACCESSION   DASSFQ010000195 DASSFQ010000000
VERSION     DASSFQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173076"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFH96_06740"
     CDS             <1..279
                     /locus_tag="VFH96_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973705.1"
                     /translation="ADVIYAKSWGSKQFYGNTDQDIANRAQYRSKWIVDEEKMARTNN
                     AIFMHCLPVRRNVIVTDAVIDSPASVVIDEAENRLHVQKAILARLLGG"
     gene            283..1191
                     /gene="argB"
                     /locus_tag="VFH96_06745"
     CDS             283..1191
                     /gene="argB"
                     /locus_tag="VFH96_06745"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET6973706.1"
                     /translation="MLETKAVGDQLRLDLLREALPYIQRFQGQTFVVKLSGKVTEDQE
                     NLNSLAEELALLHQVGIRICVVHGGGKQLSDLARKLGIEQTIIEGRRVTDDATLEMAK
                     MIFAGKINTDILAALRQRSIEAVGLSGVDGNIVHAEKRPPKEILNRETGVRDKIDFGF
                     VGDVVQINARLLTVLLDHGYLPVISSLGADDDGMVFNINADTIAAEIAVQLKAEKLIL
                     LSDVDGIYLTAGDPQTKLSRVTAAEAGELINNGSASGGMIPKLQSIIVLLERGVHSAH
                     IISGTKRNALLSEIFTDKGTGTMVHG"
     gene            complement(1257..1835)
                     /locus_tag="VFH96_06750"
     CDS             complement(1257..1835)
                     /locus_tag="VFH96_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973707.1"
                     /translation="MTFIEFADEPSQEERINKLRAELEKLGGNTSTLESMPADMEEEF
                     LRHVLEYETAEQISLLRLLENAGLEVPAPETLDDDALKIRLKELIERMASVGAYVLHT
                     NHLSDRDLYNYLYHDALREETVLFPENPSYAYMIDLTGSGGEDDNQTYLKYYADEEYR
                     RQWAHDWPNDPIPEHEEPPFDRDRFLPQSPVG"
     gene            complement(2004..2750)
                     /locus_tag="VFH96_06755"
     CDS             complement(2004..2750)
                     /locus_tag="VFH96_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6973708.1"
                     /translation="MKRRQLLTGISVAGAGVLLNACRSNTQTRTGGATNDENKEAPAP
                     GEAEPVEVTATEDLMREHGILRRALLVYQESAVRLRQDPASVPPEALEKTAKLFRVFG
                     EDYHEKQLEETFIFPAVKKTTNAATPYVDILLAQHVRGREITDYLLSISQADKIASNS
                     IEPLAKTLESFVRMYEHHAAIEDTVVFPAWKAAVGGNELDALGEKFEEIEHDQFGSDG
                     FETALKRMEEIEQSLGLSNLDMFTAPAPPK"
     gene            2802..3254
                     /locus_tag="VFH96_06760"
     CDS             2802..3254
                     /locus_tag="VFH96_06760"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET6973709.1"
                     /translation="MDNKEIARRFYRLSALMEVRGDDPFRLRSYRNAAEAIEVWPTSL
                     KEIADQEGVTGLQEIPGIGKAIAGKVIELLTKGTFDAWERLTAETPETVLDLLEIPGI
                     GPKTAALLHQRFKVSSLPDLKTFAAGGGLDMVDGIGPKTAERIKESLS"
     gene            3308..4426
                     /locus_tag="VFH96_06765"
     CDS             3308..4426
                     /locus_tag="VFH96_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HET6973710.1"
                     /translation="MKKMKRRDFMRNGAVAGLTLAAAKNEVAGFPMILTQSAVKPLVI
                     SSANGNHFKNGGSVTAVQKAFTMITGGADVLDAVIAGVNIVELDPLDDSVGYGGLPNA
                     DGVVQLDSSVMHGPKKRAGAVGALEGVRTPSLVARAVLEETDHHLIVGKGAQDFARNM
                     GFKIEDDLNTENSRKQWLEWKRRSDPQHYLDPKKRAEAGHRVALEMLAEGKLREENLH
                     GTINCDGINSRGEICGVTTTSGLAWKIPGRLGDSPILGAGLYVDGAVGAAGSTGRGEA
                     NLFNLCSFVIVEEMRRGAHPKDAGMEVLNRIKNNTVEKRLLNGRGLPNFGINFYILNA
                     KGEYAGVTMYDGASFAICNENGPQTLKSEPLLQGKPSD"
     gene            complement(4458..4820)
                     /locus_tag="VFH96_06770"
     CDS             complement(4458..4820)
                     /locus_tag="VFH96_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973711.1"
                     /translation="MSKPLIGVILGAVLGAIDGLTAWFTPAVRDQLFGIVFGSTIKGI
                     IAGVAIGFFAKKFHSLPIGILFGVIVGAILAWLVAFMQGGYYFQIILPGTLVGLIVGF
                     ATQKYGATDKHGLVADQR"
     gene            complement(4881..>5106)
                     /locus_tag="VFH96_06775"
     CDS             complement(4881..>5106)
                     /locus_tag="VFH96_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973712.1"
                     /translation="ITSKATATAKESRYATASELSADIARLLDAEPVSAYRENAFERT
                     SRWVGKNRFLVLLVLAYLLMRIFLIFTSRS"
BASE COUNT         1182 a         1401 c         1430 g         1093 t
ORIGIN      
        1 gcagacgtta tttacgcgaa gagctggggc tcgaaacagt tttacggcaa cacggatcag
       61 gacatcgcga atcgcgcgca ataccggagc aagtggatcg tcgatgaaga aaagatggcg
      121 cgcacaaaca acgcgatctt catgcactgc ctgccggtgc gccgaaacgt gatcgtgact
      181 gacgctgtga tcgattcgcc cgcgtccgtc gtgatcgatg aagctgagaa tcgtctgcac
      241 gtgcagaaag cgatactcgc aaggttgttg ggaggttaga ccatgctcga aaccaaagcg
      301 gtcggcgatc agcttcgttt ggatctgttg cgcgaggcgt tgccctatat ccagcgcttc
      361 cagggacaga cgtttgtcgt caaactctcg ggcaaagtca cggaagatca ggagaatctg
      421 aactcactcg ccgaggagct ggcgttgttg caccaggtcg gtattcgcat ctgtgtggtg
      481 catggcgggg gcaaacaact gagcgacctt gccaggaagc tcggaatcga gcagacgatc
      541 atcgagggcc ggcgcgtcac tgacgacgcg acgctcgaaa tggcgaagat gatctttgcc
      601 ggaaagatca atacagacat tctggcggca ctccgccagc gcagcatcga agcggtggga
      661 ctctcgggcg ttgacggcaa catcgtccat gccgagaagc gcccgccgaa agagatcctg
      721 aaccgcgaaa cgggcgtgcg cgataaaatc gatttcgggt ttgtcggtga cgtggtccag
      781 atcaatgcgc gactattaac tgtcttgctg gatcacggtt atttgccggt gatctcgtca
      841 ctcggtgcag acgatgacgg catggtgttc aacatcaacg ccgatacgat cgccgccgag
      901 atcgcagttc aactaaaggc cgagaaactc atcctgctca gcgatgtcga cggaatttac
      961 ctcaccgcgg gcgatccgca gacaaaattg tcccgtgtca ccgcagccga agccggtgag
     1021 ttgattaata atggctcggc ttcgggtggg atgatcccga agcttcagag catcatcgtc
     1081 ttgttagagc gcggcgttca cagcgcacac atcatcagcg gcaccaaacg taatgcactc
     1141 ctgtccgaga tcttcacgga caaaggcacc ggcacaatgg tccacggata atacggattt
     1201 ttaaaaacag atctgtgcag atcagaaata aatccgtatt atccgtggct aaaaaatcac
     1261 cccaccggtg actgtggcag gaaccgatcg cgatcgaacg gcggctcctc gtgctcgggt
     1321 atcggatcgt tgggccaatc atgcgcccac tgccttcgat actcctcatc cgcgtaatac
     1381 ttcaggtaag tctggttgtc gtcttcgccg ccactgccag tcaggtcgat catgtaagcg
     1441 taactgggat tctcgggaaa cagcaccgtc tcttcacgca acgcatcgtg gtaaagatag
     1501 ttgtacaaat cgcgatccga aagatggttc gtatgcagga cataagcacc gactgacgcc
     1561 atgcgttcga taagctcttt cagccttatc ttcaacgcat cgtcgtcgag cgtctcaggc
     1621 gccggcactt ccaacccggc attctcgagc aaacgcagca gactgatctg ttcggcggtt
     1681 tcgtattcga gtacgtgccg caggaattct tcttccatgt cagcgggcat cgactcgagt
     1741 gtggacgtat tgccgccgag tttttccagt tcagcgcgga gtttgttgat gcgttcttcc
     1801 tggctgggct cgtcagcgaa ttctatgaat gtcatgttgt ggctccttaa ggttagaggt
     1861 tgaggtttga ggtgccttgg gagcgtgtga ggccctccgt ccgctttgag gggtagcgca
     1921 ttgtcgtctc tgcgtgagga aaagtaaaga ccatccacag atacacagaa ctttttaact
     1981 atctgcgtaa tctgtggatg gttttattta ggtggtgcgg gcgcggtgaa catatccagg
     2041 ttcgacaagc cgagactctg ctcgatctct tccattcgct tcagcgctgt ctcgaatcca
     2101 tcgcttccaa actgatcgtg ctcgatctct tcaaacttct cgcccagcgc atcgagctca
     2161 ttcccgccga cggcagcctt ccacgccgga aacacaaccg tgtcttcaat cgcagcatga
     2221 tgctcataca tgcgcacaaa cgactccaac gtcttcgcga gcggctcaat actgttcgac
     2281 gcaattttat ccgcctgtga gatcgacaac aggtaatccg tgatctcgcg tccgcgcaca
     2341 tgctgggcca gcaatatatc gacgtacggc gtcgccgcgt tcgtcgtctt ctttaccgcc
     2401 ggaaagatga acgtctcctc caactgcttc tcgtggtaat cctcgccgaa cactcgaaac
     2461 agctttgccg ttttttcgag tgcttcgggc ggcactgatg cgggatcctg ccgcaaacga
     2521 acggccgact cctggtagac cagcagcgcg cgtcgcagga tgccgtgttc gcgcatcaag
     2581 tcttcagtcg cagtcacctc aaccggctca gcctcacccg gcgccggcgc ttctttattt
     2641 tcatcattcg tggcgccgcc ggtgcgcgtt tgtgtgttac tacggcaagc attgagcaaa
     2701 actccggcgc ccgcgacgga aatgcccgtt agcagctgtc gtcttttcat gtccatgcct
     2761 ttgatattac tctgcagacg acgttagact aagcaaaatt aatggacaac aaggaaattg
     2821 cccgccggtt ctatcgtctt tctgcgctca tggaagttcg tggcgacgat ccatttcgtc
     2881 tgcgctcgta tcgtaacgca gccgaagcga tcgaggtgtg gccgacgtcg ctgaaagaga
     2941 ttgcggatca ggaaggcgtc acaggcttac aggaaatccc gggtatcggc aaagcgatcg
     3001 ctggcaaagt gatcgagtta ttgacgaagg gaactttcga cgcgtgggaa cgactcaccg
     3061 ccgagactcc ggaaaccgta ctcgaccttc tggaaatccc cggcatcggc ccgaagacgg
     3121 cggcgctcct ccaccagcgc ttcaaagtct cctcccttcc cgatctaaaa actttcgcag
     3181 ctggcggcgg tctcgacatg gtcgacggca tcggccccaa aactgcggaa agaatcaaag
     3241 aatcactttc ctgatcagtg tcatctgcgt taatctgtgg cccactctcc agataaacga
     3301 ggacaacatg aaaaaaatga aacggcgcga tttcatgcgc aacggggccg tggctggatt
     3361 gactttggct gccgccaaaa atgaagttgc gggttttccg atgattttga cgcagagcgc
     3421 ggtgaagccg ctcgtgattt cgtctgctaa cgggaatcac ttcaagaatg gcggaagtgt
     3481 gacggcggtg cagaaggcgt tcacgatgat tactgggggc gctgatgtgc tcgatgccgt
     3541 gattgcgggg gtgaatatcg tcgaactcga tccgctcgat gacagcgtcg gctacggcgg
     3601 gttgccgaat gctgacggcg tggtccaact cgattcttca gtgatgcacg ggccgaaaaa
     3661 acgcgccggc gccgtgggag cactcgaggg tgtacgcacg ccgtcgctcg tggccagggc
     3721 ggtgctggaa gaaaccgatc accatctcat cgtcggcaaa ggcgcgcagg atttcgcgcg
     3781 caacatggga ttcaagatcg aagacgatct caacactgag aactcgcgca aacagtggct
     3841 cgagtggaaa cgacgctcag atccacaaca ctatctcgat ccgaagaagc gcgctgaagc
     3901 tggtcatcgc gtcgcgctcg agatgctcgc ggaaggaaag ctacgcgaag aaaacctgca
     3961 cggtacgatc aactgcgacg gcatcaactc gcgcggggag atctgcggcg tcactaccac
     4021 cagcggactc gcgtggaaaa ttcccgggcg tctcggtgat tctccgatcc tcggcgcggg
     4081 gctatatgtc gatggcgccg tcggcgcggc gggctcgacc ggacgtggtg aagcgaatct
     4141 cttcaatctc tgctcgttcg tgatcgtcga agagatgcga cgcggggcgc atccgaaaga
     4201 cgcaggcatg gaagtgctga accggatcaa gaacaacacg gtggagaaga gattgctgaa
     4261 cggccgcggc ttgccgaact tcgggatcaa cttctacata ctgaatgcga agggtgagta
     4321 tgcgggagtg acgatgtatg acggagcgtc gtttgcgatc tgtaacgaga atggtccaca
     4381 gacactcaaa tcagagccgc tgctgcaagg caaaccgagc gattagccac ggataacacg
     4441 gattttaaaa acccagatca gcgctgatca gccaccaatc cgtgtttatc cgtggccccg
     4501 tacttctgcg tcgcgaagcc gacgatgagg ccgacgagcg tgcccggcag gatgatctgg
     4561 aagtagtaac cgccctgcat gaacgctacg agccacgcga ggatcgcacc tacaatcacg
     4621 ccaaacaaaa tcccgattgg caacgagtga aacttcttcg cgaagaagcc gatcgcaacg
     4681 ccggcgatga ttcctttgat cgtggaaccg aagacgattc cgaaaagctg atctctcacg
     4741 gccggcgtga accacgcagt caggccgtcg atggcgccca ggacggcacc gagtattact
     4801 ccaatcaggg gcttggacat cgtttttctc cttctattca gatcggtctg ttcttctatt
     4861 aaacgtctgt cgagaaatct ttaacttcga gaagtaaaaa tcaaaaaaat tctcatcagc
     4921 aggtaagcga gcacgagcaa caccagaaag cggttcttac caacccagcg actcgtgcgt
     4981 tcaaaagcat tttcgcgata tgcggagacg ggctcagcat cgagcaagcg agcgatatcc
     5041 gccgagagtt ctgatgcagt cgcgtaccgg ctctccttcg cggtcgctgt ggccttcgaa
     5101 gtgatc
//
LOCUS       DASSFQ010000196        13929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1281637, whole genome shotgun sequence.
ACCESSION   DASSFQ010000196 DASSFQ010000000
VERSION     DASSFQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281637"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFH96_06780"
     CDS             <1..911
                     /locus_tag="VFH96_06780"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6973713.1"
                     /translation="ITVIARTGEYAIEHADHEPKKRGHVVAVFSPKGGCGTSLIATNL
                     AMTQKSPTVLVDLNLQSGDLELLLGLKPKFSLADVVENRDRLDDALLASYITPHSKNL
                     SLLAAPTKAESADDIEPKHIYEVMELLRQRYDNVIIDTPHSFDSVTISALDHADQILV
                     VLTLEIHAIRSTRRALEIFDRLGYTRKKVRLVVNRYSKNIELDQKQVEDFLGERVVAF
                     IQSDYKAAVNSINLGQPLVDSAPGSLVTTDLFNLASKVFADKVEPPAPTAGGRRPFNS
                     LFKRSSTSMQDLGLNAALERANLSEG"
     gene            1007..1285
                     /locus_tag="VFH96_06785"
     CDS             1007..1285
                     /locus_tag="VFH96_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973714.1"
                     /translation="MALRERLVRESGMTGALADDVISNQQGFQDMKARLHRMLINRMD
                     LTKLGQLEPEQLHEEVARLASSLLAQEDTPLSTVERERLIHEVRHELF"
     gene            1355..2341
                     /locus_tag="VFH96_06790"
     CDS             1355..2341
                     /locus_tag="VFH96_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET6973715.1"
                     /translation="MYIERGGKLEKTSIEFKDNEHLMRVIERIVSSVGRRIDEASPMV
                     DARLKDGSRVNAIIPPLSLDGPVLSIRRFGAEPLRMHRLLENQALTPDMAAMFEMCVN
                     AKLNVLISGGTGAGKTTLLNALSAYIPADERIVTIEDSAELQMQQPHVVRLETRPPNI
                     EGKGEVSQRDLVKNALRMRPDRIVIGEVRGGEAIDMLQAMNTGHDGSLTTVHANTPRD
                     ALSRLETMIQMTGMRLSDRAMRQQIASAINLVIQVARLTDGTRRITSISEITGMEGET
                     ITMQEVFIFDRRGVDKDGKVLGKFRPTGVRPRFAERLKIYGMQLPRSFFEEV"
     gene            2347..3315
                     /locus_tag="VFH96_06795"
     CDS             2347..3315
                     /locus_tag="VFH96_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6973716.1"
                     /translation="MFLILFFVFLTCLFLTYTLYLFTSRASDKKRALLKERLADAIRT
                     SAHSTDMDVQLARQELMSEIPWMNRVLIKLQVSSKLKQMIDQADSHITVMRLILFSLT
                     AGVLAALAVSMISTSYLLMGFFGFVATALPFLHIHSKRKKRLKKFLQLLPDALDLMSR
                     GLSAGHAFTEALHMVATEMPEPISTEFRKTYEEQNLGLSLKLALQNMVERVPLLDLRM
                     CVTAVLIQRETGGNLSELLEKVAYTIRERFRIMEDLKTMTLSSRWSAWLLCGLPIFLA
                     VYVTIMNPGYMDVMWRDARGNRLLALAAIMQVLGMLMVQRIMKIRI"
     gene            3318..4262
                     /locus_tag="VFH96_06800"
     CDS             3318..4262
                     /locus_tag="VFH96_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6973717.1"
                     /translation="MVTIILISTFICIAGAVMAIYWLLFRPVSATAQRLSDLDEGRVG
                     VIAPSIEVSGMETLAARLAEPINKLVPPSAAGAKKLQRELMQAGYRSPAAPAIYRVSQ
                     LFSMLFFPTLIVFVWTVVMVHPLNDALLPALFAFGAGFILPRFILNRKIANRKLRITW
                     GLADALDLMVITMEAGLGLNAAMLRVCEELRPVHPDISKEFELANLEIRVGRERSEAL
                     RNLADRTGVDDLNSLVGMLIQADRFGTSIARAVRVYSDSLRTKRRQRAEQAAQKAAFK
                     LLLPLGALLFPTMFIVILGPALLNISDMLSGSGGGVTF"
     gene            4281..5279
                     /locus_tag="VFH96_06805"
     CDS             4281..5279
                     /locus_tag="VFH96_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973718.1"
                     /translation="MAKRSLIVFALTLVLVTAAVPVRHVHGVLMTPEEVAMLDAASRD
                     TDDQSKGDNTFVRVLKAPFKAIGRLFGTGKKDDDKPQRLSRKDVKKFETIGAAKVVDA
                     RTTGFETPVASTTEKVDPAAAALAPAQANLERGRALLNSGNATDAIAVLSTAVSTDPT
                     LHEAHNLLGVAYEMKGLRDRAFESFEKALKADNDNGEYLNNLGFLYFKNGDNDKAAKY
                     LKRAVKVAPQAQRYWNNLGLVQAQRAKFDEAYDCFVRAVGEYQGHINVANRSQTMGYD
                     KIAIKHLEQARVLRPATAEILLRLAVLYKRTGNEELAAEANAALVAAKSQANATKE"
     gene            5387..6661
                     /locus_tag="VFH96_06810"
     CDS             5387..6661
                     /locus_tag="VFH96_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973719.1"
                     /translation="MLEELADSGIHEQFLCDLALKHVAMLPDPNTTAVADRLHLPRVL
                     TEELLYHLYREKLIEMRLQSAVGSTRYAMLDHGWERVTRLNAQSGYLGPAPVSLEDYA
                     LMMRLQAVPSKPASFDTVRNAFKDLVLPDSLLQTLGCVINSRSSLFLTGLPGTGKTAV
                     SERMNQALFGSIWIPHAIEVDGNIIRIYDAHCHHPVPERTSIIEYDRRWIEIQRPLII
                     VGGELTLDNTDLAFSETARFYEAPFQVKSNGGTLVIDDFGRQRVSPQDLLNRWILPLE
                     RRVDFLTLHSGKKIEVPFEQLVVFSTNLDEKDLVDEAFLRRMGYRARVEPPTPAAFSE
                     IFKRTAKARGVAIDEECLRHILHKYVDENRIMKGCEPRDLLNKVNDICLFEGRDLRLT
                     RELVDLAWGNYFGTSHGFAPEAAKFSSNGSHG"
     gene            6719..7621
                     /locus_tag="VFH96_06815"
     CDS             6719..7621
                     /locus_tag="VFH96_06815"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6973720.1"
                     /translation="MLSALAIHAQDKAAKTPPKIIGYTVTLVDGYTFDVDEVSRQGDE
                     TWVRKGNIQRQVQGPIKSIKPILEQPKAEVAEAPKTVAPSTTTTTKPAGPNPIWIYLV
                     DGAKFRVDQVQEQTDGAWYSRQNVSIFLAKERISRIEYEAPNTPAATTKNADWTSGSP
                     VIDQLIRTHANRVGIDPYLVFLVIEKESRFHTRAVSPKGAMGLMQLMPGTARRLGVRK
                     PFDAAENIRGGTQYMKELMDMFGGKVDLVLASYNAGEGAVLKYGRNVPPYRETRDYVK
                     TIGKRYGLTGRQPGAQTDVPEPQR"
     gene            7633..8376
                     /locus_tag="VFH96_06820"
     CDS             7633..8376
                     /locus_tag="VFH96_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973721.1"
                     /translation="MKSFFPTILITLLLLAGSALGVQAQTKDEGPMTNAEVIKLVKAG
                     FKEKTIVLIIASRIPNFDLTSDQMIKLKRAGVNENIIVAMLARQEGTDVPADDSSWGD
                     DPFFNSRLDPKDGSVKSSGSGSGSDGGTNIFGSSSGAREKNSTRTTNGSASGDSQLSG
                     SATVRLIKPPSEAGGGTAAKLEKTKSLTNESIVELVEAGFSEGTIIRRIEQSPVEFDL
                     AADKLDALRKKRVTDKVIVAMKAAMGEDK"
     gene            8404..9534
                     /locus_tag="VFH96_06825"
     CDS             8404..9534
                     /locus_tag="VFH96_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973722.1"
                     /translation="MRVKSLRWIALLVCAALLFSAASAQRKGQRQTKPQPKPKATPAP
                     QSSLETMGAPPPVPSLKKPPEQEVSPGDVVSVDTTEVMFPVTVRDSNGRLVNELTRND
                     FRVFEDGAMQPLSDLALRQVPVDVVLMVDASSSVANNLDDFRRAAQGFAARLEADDRL
                     SLIKFDDRIELLQDWTKSRFQLQRALGRIEPGMFTRFNDALQLAAKEQFGPVTKSRRA
                     VIVLSDGIDNGQGTTSLETALTSLIRAQVAVYIVANTEISRAAKRAELDTLLGGTESS
                     IKFNQVNIDRLRLGLKVLDQSEENLAQLAKMTGGRLYKPQTFDALESTYAEVAEELRH
                     QYALYFTPLNKARDGSFRKVRVEMTNPALYTQTRVGYFAPKS"
     gene            9592..10146
                     /locus_tag="VFH96_06830"
     CDS             9592..10146
                     /locus_tag="VFH96_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973723.1"
                     /translation="MLSRLPYCLIVLALSLTYVYAQETKDKVTIVLATGNTLLVDEVS
                     ESSDGYWYKRGNITTFLDRDRVTRIEHPKPEGEAATPKPARESKWTLAEATKVEQFFV
                     SKFKRPLPLTAFGQSDLHTRWGLDHRNGMDVGLHPDSVEGRALIEFLRAESIPFLVFR
                     GPVPGVATGPHIHVGNRSSRSYGR"
     gene            10182..10394
                     /locus_tag="VFH96_06835"
     CDS             10182..10394
                     /locus_tag="VFH96_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973724.1"
                     /translation="MMKFLEFPHPEGGRMIVHVDHITSAIYRKGEGDVKTRLGLDLDQ
                     RQNDIVIFGDDAEDAWAALQRLKDES"
     gene            10391..12112
                     /locus_tag="VFH96_06840"
     CDS             10391..12112
                     /locus_tag="VFH96_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6973725.1"
                     /translation="MNQDSKKRTSSNDPLSEQWIDLRLIAGMRVVLGTCALFVLLIGP
                     MRTLPGYMPIYFTLVFYSLYGLVIYHLSVRRNPLVAHKAVPWLDLLWYVALIAFTSNI
                     NSLFYYFFFYSIIVASFSWGLTEGLRLTLASAAIFTTVSILTAPKDRPLDLDNLLIAP
                     IGLLIFGYIIARWGGYHTELKNRLKLLKEVTIFSNPRFGIDRTIKAIMESIRAFYDAD
                     TCVLVVPGKPGDADSYLMYRVTRGTHLSRSAPPEIGHEAAEQFLSPSLDFAVIYKKNG
                     HAYTRLFDVKSRVFSDTNSSESEKLAGMLDAQKYLSVPVYYRHQAVGRLYIIDGPERI
                     DSAAMDFMLQLMDHVTPVMENIRLIDNLASDAAEQERQRIAHDIHDSVIQPYLGLQFG
                     LVALDQKLEAGQLGIRDNVQELLELTNHELAELRRFVWGLRANEERTDVLLPAIERYA
                     ERFSMVTGIKVDVAAKGKVKVNDRLAAELFQIVAEGLSNVRRHAFCRDARVEISCKET
                     SLLLQIKNSRPSKGVNPEGERVDGNEGVKPFRPHSIAERAASLGGDTHVFVDDKDYTV
                     VSVGIPL"
     gene            12128..12793
                     /locus_tag="VFH96_06845"
     CDS             12128..12793
                     /locus_tag="VFH96_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6973726.1"
                     /translation="MSSRPITIMIIDDHVVIRSGLRMLIEYDQRMQVVAQAGSKVEAL
                     ERAAATNPDVIILDLLLGDDDGLSFLPELCQASPNSRVLVLTGVQNADAHRRAIRRGA
                     MGIVLKEHAADKLLKAIMKVSEGEVWIERSMMGSMIQEFNKPALVDPEVSKIESLTDR
                     EREVIALIGEGLKNKQVGERLFISETTVTHHLSSVFSKLEVSDRLELIIYAFRHGLAK
                     MPK"
     gene            12917..13594
                     /locus_tag="VFH96_06850"
     CDS             12917..13594
                     /locus_tag="VFH96_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6973727.1"
                     /translation="MEPAPIKIRILIVDDQLVVREGLRMLIENHPGTKVIAMASTRSE
                     ALDILARETCDLIILDLELGGYSATSFIPQLREAARNSRVLVLTGSRDTAIHQKAAQL
                     GAMGVVLKEDAADLLLKAIEKVYKGEAWLDRVTLGNLLFQMSNHDRQPLDPRAQKISS
                     LTDRERQVIALIAEGLKNRQIAERLFISPTTVTHHLSSIYSKLGVSDRLELVIYAFAN
                     KLAKMPE"
BASE COUNT         3175 a         3876 c         3864 g         3014 t
ORIGIN      
        1 taatcaccgt catcgcacgg accggagagt atgcgattga acatgccgat cacgaaccga
       61 agaagcgggg tcatgtggtc gccgtgtttt caccgaaggg tggttgcggc accagcctga
      121 tcgcgacgaa cctcgcgatg acgcaaaaga gtccgacagt gcttgtcgat ctcaatctgc
      181 aatcaggcga tctcgagttg ctgctcggtc tgaagccgaa gttttcactc gctgacgtgg
      241 ttgagaaccg cgatcgtttg gacgacgcgc tgctagccag ctacatcacg ccgcattcga
      301 agaacctttc gctgcttgcg gcgccgacga aagccgagag tgctgacgac atcgagccga
      361 aacacattta cgaagtgatg gagctgctgc gacagcgtta cgacaacgtt atcatcgaca
      421 cgccgcacag cttcgactcc gtcaccatct cggcgttgga ccatgccgat caaatcctgg
      481 tggtgttgac gctcgagatt catgcgattc gcagcacgcg ccgggcgctc gagatattcg
      541 atcgactcgg atacacgcgt aagaaggtgc gactcgtcgt caaccggtat agcaagaata
      601 tagagttgga tcagaagcag gttgaagact tcctcggcga gcgcgtggtt gcttttattc
      661 agagtgacta taaagccgca gtgaattcga tcaatctcgg ccagccactc gtcgattcgg
      721 ctcccggatc gttagtgacg acggatctct tcaaccttgc gagcaaagtt ttcgcggaca
      781 aggtcgagcc accggcgccc accgccggtg gcaggcgtcc gttcaactcc cttttcaaac
      841 gcagctcaac cagcatgcag gatcttggtc tcaacgccgc cctggagcgg gcaaatttga
      901 gtgaaggata gggtcagtgt ccgacatgct ttagcttgtc ggttacaaga cagaggaccg
      961 acaagctaaa gcatgtcgga cagggatcaa aagttaggga atcattatgg cactacgtga
     1021 acgtttagta agagaaagcg gaatgaccgg cgccctcgct gatgacgtaa tcagcaatca
     1081 gcaagggttc caagacatga aagcaagact ccaccggatg cttatcaacc ggatggacct
     1141 gaccaagctg ggccaactcg agccggagca gcttcatgaa gaagtggcgc gtctcgcctc
     1201 gagcctgctg gcccaggaag acacgccgct ctcgacagtc gagcgcgagc gtttgatcca
     1261 tgaagtgcgg cacgagctct tctgactggg accactcgag caactactcg ccgacacttc
     1321 catttccgac attctggtta actctcccta caacatctac atcgagcgcg gcggcaaact
     1381 cgagaagacg agtatcgagt tcaaagacaa cgagcacctg atgcgtgtta tcgagcgcat
     1441 cgtctcgtcg gtcggccgtc gcatcgacga agcgtcgccg atggttgacg ctcgtttgaa
     1501 agacggatcg cgcgtcaacg cgatcatccc gccactctcg ctcgacggcc ccgtgctctc
     1561 gattcgacgt ttcggcgccg agccgctgcg catgcatcgt ctgctcgaga accaggcgct
     1621 cacgcccgac atggctgcga tgtttgagat gtgtgtcaac gcaaagctca acgtcctgat
     1681 ctcaggcggt actggcgctg gtaaaacgac attgctcaat gctctttcgg cctacatccc
     1741 ggccgatgaa cgcatcgtca cgatcgaaga ctcggctgaa ttgcagatgc aacagccgca
     1801 cgtcgtgcgt ctcgaaacac gtccacccaa catcgagggc aagggcgaag tgagccagcg
     1861 cgatctcgtc aagaacgcac tgcgtatgcg acccgatcgc atcgtgatcg gcgaggtccg
     1921 cggtggtgaa gcaatcgaca tgttacaggc gatgaacacc ggtcacgatg gttcattgac
     1981 aacggttcac gccaacactc cgcgcgacgc tctatcccgt ctcgaaacga tgatccagat
     2041 gaccggcatg cgtctctccg atcgcgcgat gcgacaacag atcgcgtcgg ccatcaatct
     2101 cgtgatccag gtggcgcgcc tgacagacgg cacgcgccgt atcacctcca tctctgaaat
     2161 caccggtatg gaaggcgaga cgatcacgat gcaggaagtg ttcatcttcg atcgccgggg
     2221 tgtggacaaa gacgggaaag tgcttgggaa gtttcgacca acgggcgtgc gaccgcgttt
     2281 cgccgaacga ctcaaaatct acggcatgca gttgccacga tcgttcttcg aggaggttta
     2341 agcgtaatgt ttctaattct cttcttcgta tttctaacct gtctttttct gacgtacacg
     2401 ctgtacttgt tcacgtcgcg tgcctcagac aagaaacgag cgctactcaa ggaacgtctg
     2461 gcggacgcga tccgcacttc ggcgcactcg accgatatgg acgtgcaact ggcgcgccag
     2521 gaattgatga gcgaaatccc gtggatgaac cgggtgctga tcaagctgca ggtttcttcg
     2581 aaactcaagc agatgatcga tcaggccgac tcgcacatca cggtcatgcg cctgatcctc
     2641 ttctcgttga cggccggtgt gctcgcggcc ctcgcggtct cgatgatcag cacgtcgtac
     2701 ctgttgatgg gcttctttgg cttcgtcgcc accgcgcttc cgtttcttca cattcacagc
     2761 aagcgcaaaa aacgactcaa aaaattcctc cagcttctgc ctgacgcact cgatctcatg
     2821 agtcggggac tttctgccgg tcacgccttc accgaagcgt tgcacatggt ggccacggaa
     2881 atgcccgagc cgatttcgac tgagtttcgc aagacttacg aagagcaaaa cctcggactc
     2941 tcgctgaagc ttgcactcca gaacatggtt gagcgtgtgc ctcttctcga tctgcgcatg
     3001 tgcgtcaccg ctgtcctgat ccagcgagag accggcggta acctttccga gctgctcgag
     3061 aaagttgctt acactattcg cgaacgcttc cgcatcatgg aagacctgaa gacgatgacg
     3121 ctgagctcgc gctggtccgc gtggctactc tgcgggctgc cgatcttcct cgcagtctac
     3181 gtgacgatta tgaatccggg ttacatggac gtgatgtggc gcgatgcacg cggcaacagg
     3241 ctgctggcac tcgcggcgat catgcaggtg ctcggtatgc tgatggtcca gaggatcatg
     3301 aagataagga tctaatcatg gtaacaatca tccttatatc gacatttatt tgcatcgccg
     3361 gcgcggtgat ggcgatctac tggctgctct tccgccccgt aagcgcgact gcgcaacgtt
     3421 tgagcgacct cgatgaaggc agagtcggag ttattgctcc gagcatcgag gtcagcggaa
     3481 tggagacgct cgctgcacgt ttggctgagc caatcaataa actcgtaccg ccatccgcgg
     3541 cgggcgctaa aaaactgcaa cgcgagctga tgcaggccgg gtatcgctcg cctgccgcac
     3601 ctgccattta tcgagtgtca cagttgttct caatgctctt ttttccgacg ttgatcgtgt
     3661 tcgtctggac ggtggtaatg gtccatccat tgaacgacgc cctgctaccg gcgttgtttg
     3721 cattcggtgc cggcttcata ctgccgcgct tcatcctcaa tcgcaagatc gccaaccgca
     3781 agctgcgcat cacgtggggt ctcgccgacg ctctcgacct gatggtcatc acgatggaag
     3841 caggcttggg cttgaacgcc gcgatgcttc gcgtttgtga agagctgcgg ccggtccatc
     3901 ctgatatcag taaggaattc gagctggcga acctcgaaat tcgcgtcggt cgcgagcgca
     3961 gtgaagcgtt gcgaaatctg gccgaccgca ccggtgtcga cgatctcaac agtctcgttg
     4021 gcatgttgat ccaggcggat cgcttcggta cttcaatcgc acgcgcagta cgcgtctatt
     4081 ctgacagctt gcgcacgaag cgtcgccagc gcgccgaaca ggccgcacag aaagcagcgt
     4141 tcaaactgct gctgccgctt ggagcgctgc tgttcccaac gatgttcatc gtcattcttg
     4201 gtccagcgtt gctgaacatt tctgacatgc tgagcggtag tggtggtggt gtcactttct
     4261 aatccccaag ggaggctcct atggcaaagc gttcattaat cgtcttcgcg ctcacacttg
     4321 tactggttac cgccgcggtg cccgttcgtc acgttcatgg cgttctcatg acgccggaag
     4381 aagtcgcaat gcttgatgcg gcttcgcgtg acactgatga ccagtccaaa ggcgacaaca
     4441 cttttgtccg cgttttgaaa gcccccttca aagcgatcgg tcgcctgttt ggtacgggaa
     4501 agaaagacga cgacaaacca caacggcttt ctcgcaagga cgtgaagaag tttgagacca
     4561 tcggcgcggc gaaagtcgtc gacgcgcgca ctaccggatt cgaaacgccc gtggcttcca
     4621 ctacagagaa agtggatcct gctgctgccg cactcgcccc cgctcaggca aatctcgaac
     4681 gtggccgagc gttactcaac agcggcaatg cgaccgatgc gattgcagtg ctttcaaccg
     4741 cagtgtcaac agatccgact ctgcacgaag ctcacaacct gctgggtgtt gcgtacgaga
     4801 tgaagggact gcgcgatcgc gcgttcgagt cgttcgagaa agcgctcaaa gccgataacg
     4861 acaacggcga atacctgaac aatctcggtt tcctgtattt caaaaacggt gacaacgaca
     4921 aagccgcgaa gtatttgaag cgcgcggtga aagtcgcgcc gcaagcacag cgctactgga
     4981 acaacctcgg cctggtccag gcccagcggg cgaagtttga cgaagcttac gattgtttcg
     5041 tgcgtgcggt tggtgagtat cagggacaca tcaacgtggc caaccggtcg caaacgatgg
     5101 gttacgacaa gatcgccatc aagcacctcg aacaggcgcg cgtgctgcgt ccggcaacgg
     5161 ctgagattct tttgcgactc gctgttcttt acaagcgcac tggcaatgaa gaactcgccg
     5221 cagaggcaaa cgctgccctc gtggcggcga agtctcaggc gaacgccacc aaggaatagg
     5281 tcttatagga cgaatcggtc ctatttaact cagtttcaag gaactaaaaa gaatgggaaa
     5341 ccttagcata ttgagccgct cagaaatcga aggcttaggt ccacctatgc ttgaggagtt
     5401 ggccgattcc gggatccacg agcagttttt gtgtgacctg gccctcaagc acgtggccat
     5461 gcttcccgac ccgaatacta cagccgtcgc cgacaggctt cacctgccgc gcgtacttac
     5521 agaagaactc ctttaccacc tgtaccgcga gaagttgatc gaaatgcggt tgcagagcgc
     5581 agtgggctcg acccgttacg ccatgctcga tcacggctgg gaacgtgtga cgcgtctcaa
     5641 tgcgcagagc ggctatcttg gaccagcgcc tgtttcgctc gaagactacg cgttgatgat
     5701 gcgcttacag gcagtgcctt ccaagccggc gtcgttcgac accgttcgca acgcattcaa
     5761 ggatctcgtg ttgccggatt cgctcctgca aacgctcggt tgtgtgatca actcacgctc
     5821 gtcactcttc ctgaccggcc tgccgggaac tggaaagaca gccgtctcag aacgcatgaa
     5881 ccaggcgctc ttcggctcga tctggattcc acatgcgatc gaagtcgatg gcaacatcat
     5941 tcgtatttac gatgctcact gccatcatcc ggtgccggag cgaacgtcga tcatcgaata
     6001 cgatcgtcgc tggatcgaga ttcagcgacc actgatcatc gtcggtggtg agctgacact
     6061 cgataacact gacctcgcgt tctccgaaac ggcgagattt tacgaagcgc cttttcaggt
     6121 gaagtcgaat ggtggcacac tggtcatcga cgacttcggc cggcagcgcg tatcgcctca
     6181 agacctgctg aatcgctgga ttttgccgct cgagagacgg gttgattttc tgacgctgca
     6241 cagtggcaag aagatcgaag tgccgttcga acagctcgtc gtgttttcga cgaacctcga
     6301 cgaaaaggac ctcgttgacg aagcgttcct gcgacgcatg ggttatcgcg cgcgcgtcga
     6361 gccgccaacg ccggctgcat tttcagagat cttcaaacgc acggcgaagg cgcgtggcgt
     6421 tgcgatcgat gaggagtgcc tgcgtcacat tctccataag tacgtggatg agaatcgcat
     6481 catgaaggga tgcgagccgc gtgacctgtt gaataaagtt aacgacattt gtctcttcga
     6541 gggacgcgac ttgagattga ccagagagct cgttgatctc gcgtggggca actacttcgg
     6601 cacgtcgcac ggttttgcac ctgaggcggc gaagtttagc tcgaacggat cacacggata
     6661 acggatagta gttatgtgcg gacagtggaa acagattacg tgtgtacttg tgctgtgttt
     6721 gctgtccgct cttgcgatcc acgcgcagga caaagctgct aaaaccccac caaagatcat
     6781 cggttatacg gtcacgctcg ttgacggcta cacattcgac gttgacgaag tttccaggca
     6841 gggcgacgag acctgggttc gcaagggaaa tatccaacgg caggtccagg gaccgatcaa
     6901 gtcaatcaaa ccgatactcg agcagcccaa agccgaagtg gcggaagctc ccaaaactgt
     6961 agccccttcc acaacaacaa ccaccaaacc tgccggtccc aacccgatct ggatctatct
     7021 ggtcgacggc gcgaaattcc gtgtcgatca agttcaggaa cagactgacg gtgcctggta
     7081 cagccgtcaa aacgtctcga tttttctcgc caaagaacga atctcccgga tcgaatacga
     7141 agctccgaac acgcccgcgg ccacgaccaa aaatgccgac tggacttccg gaagtcccgt
     7201 aattgaccag ttaatccgca cacatgcgaa ccgtgttgga atcgaccctt atttggtctt
     7261 tttagtaatt gagaaagaat cgcggttcca cacgcgcgca gtcagtccaa aaggggcaat
     7321 gggcctgatg caactgatgc ccggcacagc ccgccgttta ggcgttcgca agcctttcga
     7381 cgcggctgaa aacatcagag gcggcaccca gtacatgaag gagctgatgg acatgtttgg
     7441 ggggaaagtc gatctggtgc tggcgagtta taatgcgggc gaaggcgcgg tgctcaagta
     7501 tggtcgcaac gtccctccct accgagaaac gcgcgattac gttaagacga ttggtaaacg
     7561 atacggactg acgggccggc aaccgggagc acaaaccgac gttccggaac cacaacgctg
     7621 agggggtgcg cgatgaaatc tttcttcccc acaattttaa tcactttact gctactggcc
     7681 ggatcggctc ttggtgtgca ggcgcaaacc aaagacgaag gtccaatgac gaatgctgaa
     7741 gtaatcaaac tcgtcaaagc cggcttcaaa gaaaaaacga tcgtgctgat catcgcttcg
     7801 cgaattccga actttgatct cacatccgat cagatgatta aactcaagcg cgccggggtg
     7861 aatgaaaaca tcatcgtcgc gatgcttgct cgccaggaag gtactgacgt tcccgctgac
     7921 gattcgtcgt ggggtgacga tccgttcttc aattcgagac tcgatccgaa agacggaagc
     7981 gtcaagagct ctggctctgg ctctggctct gatggcggaa cgaacatctt tggctccagc
     8041 agtggagcac gagagaagaa cagcacccgg accacaaacg gttccgcgtc tggtgacagt
     8101 caactttccg gcagcgcgac agtgcgtctt atcaaaccgc cgagtgaagc cggaggcggc
     8161 acagccgcga aactcgagaa gaccaagagc ctgacgaatg aatcgatcgt tgagctcgtg
     8221 gaggcaggtt tttcagaagg aacaattatt cgtcgcatcg agcagtcgcc ggtcgagttt
     8281 gatctggccg ccgacaagct ggacgcgctt cgtaaaaaac gtgttactga caaggtcatc
     8341 gtagcgatga aggctgcgat gggtgaggac aagtaaatct gtgtaaaccg tggttaagga
     8401 cgtatgagag taaaatcact tcgttggatc gcgctgcttg tttgtgcggc gcttttattt
     8461 tccgccgcga gcgcgcaacg gaaaggccag cgacagacta agcctcaacc aaaaccgaag
     8521 gctacgcctg ccccacagtc gtcgctcgaa acaatgggag cgcctccgcc ggttcccagt
     8581 ttaaagaaac cacctgaaca ggaagtcagt cccggtgacg ttgtttccgt cgatacgacg
     8641 gaagtgatgt ttccggtgac ggtgcgcgat tcaaacggac gtttggtcaa cgagctgacg
     8701 cgtaacgatt tccgtgtgtt tgaagacggc gcgatgcagc cgttgagcga tcttgctttg
     8761 cgacaggtgc ccgtcgatgt ggtgttgatg gtcgacgctt cgtcgtccgt cgccaacaat
     8821 ctcgacgact ttcgccgcgc cgcgcagggt tttgcggcac gtctcgaagc ggacgatcgc
     8881 cttagtctga tcaagtttga cgatcggatc gagctgctac aggattggac caagagccgg
     8941 ttccagttgc agcgcgcgct gggtcgcatc gaacccggta tgttcacgcg gttcaatgat
     9001 gcgttgcagc ttgctgcgaa agagcagttt gggccggtga cgaagtcgcg tcgcgcagtg
     9061 atcgtgttgt ctgatggcat cgataacggc caggggacca caagtctcga aactgcgttg
     9121 acttcgctga tcagggcgca ggtagcggtt tacatcgtcg ccaataccga gatctcgcgc
     9181 gcggccaagc gggctgagct cgatacactg ctgggcggaa cggagtcgtc gatcaagttc
     9241 aatcaggtga atatcgatcg tttgcggctc ggtttgaagg ttctcgatca gagtgaagag
     9301 aacctggccc aactcgcgaa gatgaccggc ggacgattgt acaagccgca gacgtttgat
     9361 gcgcttgagt cgacttatgc ggaagtggcg gaagagctgc gacaccagta cgcactttac
     9421 ttcacaccgc tgaacaaagc gcgggatgga agttttcgta aagtgcgcgt cgagatgacg
     9481 aatccggccc tttacacaca aacgcgcgtc ggctacttcg ccccgaagag ttaagacttt
     9541 catccacaga tgacacagat aatctgcgta atctgtggac aaaggctcgt catgttgagc
     9601 agactgccct attgcttaat cgttctcgca ctcagcctta cgtatgtcta cgcgcaggaa
     9661 accaaagaca aggtcacgat cgtgctcgcc accggtaaca cgctcctcgt cgatgaagta
     9721 tccgagagct ccgatggcta ctggtacaaa cgcggtaaca tcaccacgtt tctcgatcgc
     9781 gaccgcgtca ctcgcatcga acacccgaaa cctgaaggcg aagctgccac gcccaagccc
     9841 gcccgcgaaa gcaaatggac gctcgcggaa gcaaccaaag tcgagcagtt cttcgtctcg
     9901 aagtttaagc ggccgttgcc gctcacagct ttcggacagt cagatttaca cacacgctgg
     9961 ggactggacc atcgcaacgg tatggacgtt ggtctgcatc ccgacagcgt tgaaggtcgt
    10021 gcgttgattg aatttttgcg cgccgagtcg atcccgtttt tggtttttcg cggcccggtc
    10081 cccggagtgg ccaccggacc tcacatccac gtcggaaaca gatcatcgag atcctacggc
    10141 cgttaaccgg gacaatttgt ttcacccttt tgaggactga agtgatgaaa ttcctcgagt
    10201 ttccccatcc tgagggcggt agaatgatcg tccacgtcga tcacatcacg agtgcgatct
    10261 atcgtaaggg cgaaggcgac gtcaaaacgc gcctcggcct cgatctcgat caacgccaga
    10321 acgacatcgt gatcttcggc gacgacgccg aggatgcgtg ggctgcgctg cagcggctca
    10381 aggacgagag ttgaatcagg attctaaaaa acgaacctcc tccaacgacc cactctccga
    10441 acagtggata gatcttcgtc tgatcgccgg catgcgcgtc gtgctgggca cttgcgcgct
    10501 gttcgtcctc ctcattggac caatgcgaac actaccaggg tacatgccga tctacttcac
    10561 cctggttttt tactcgctgt atggtctggt gatctatcac ctgtcagttc gtcgcaatcc
    10621 actcgttgca cacaaggcgg ttccctggct cgacctgctt tggtatgtgg cgctcatcgc
    10681 ttttaccagc aacatcaaca gtctctttta ctactttttc ttttactcga tcatcgttgc
    10741 gtcgttcagt tggggactca ccgagggctt gcgactgacg ctcgcgtccg ctgcaatctt
    10801 tactaccgtc agcatactca ccgcgcccaa agaccggcca ctcgatcttg ataacctgct
    10861 gatagcgccg attggtttgc tgatctttgg ttacatcatc gcgcgctggg gcggctatca
    10921 caccgagctg aagaatcgtc tcaaactgtt gaaggaggtg acgatctttt ccaacccgcg
    10981 cttcggcatc gatcggacca tcaaagccat catggaaagc attcgtgctt tctacgatgc
    11041 cgacacgtgc gtgctcgtcg ttcccggcaa acccggtgac gctgatagtt acctgatgta
    11101 ccgcgtgacg cgtggcacac atctgagcag atccgcgccg cctgagatcg gtcacgaggc
    11161 cgccgagcag tttctctccc cttctctcga tttcgccgtc atctacaaga aaaacggtca
    11221 cgcctacaca cgcttgtttg acgtcaagtc gcgcgtgttt tctgacacaa actccagcga
    11281 gagcgaaaag ctcgccggca tgctcgacgc gcagaaatat ttgtctgtgc ccgtttacta
    11341 tcggcaccag gcagtgggcc ggctttacat catcgatggt ccggaacgga tcgacagtgc
    11401 cgccatggat ttcatgttgc agttgatgga ccacgtaacg cccgtgatgg aaaacatccg
    11461 gctcatcgac aatctcgctt ccgatgccgc ggagcaggag cgccagcgga tcgcgcacga
    11521 cattcacgac agcgtcattc agccttatct cggtttgcag tttggtcttg tcgcactcga
    11581 tcaaaagctc gaagccggcc agctcggcat tcgcgacaac gtgcaggagt tgctcgagct
    11641 aaccaaccac gagctcgccg agctgcgacg ttttgtgtgg ggacttcgcg caaacgaaga
    11701 gcgaactgac gttctgctac ccgcgatcga gcgctatgcc gagcgctttt caatggtcac
    11761 cggaatcaaa gttgacgtgg ctgcgaaggg aaaagttaag gttaacgacc gtctcgccgc
    11821 tgagttgttc cagattgtcg ccgagggttt gagcaacgtc aggcgtcacg ccttttgtcg
    11881 ggacgcgcgg gttgagatat cgtgtaagga gacctcgctg ctgttacaga ttaaaaacag
    11941 ccggccaagc aagggcgtca atcccgaagg cgaacgcgtc gatgggaatg aaggcgtaaa
    12001 gccgtttcgg ccccattcga tcgcggagcg cgctgcctct ctcggcggag acacgcatgt
    12061 gtttgttgat gacaaagact acaccgtagt tagtgtcgga atccccctgt agttttggag
    12121 tttcatcatg agttctcgtc ctatcacgat catgataatt gatgatcacg ttgtaatccg
    12181 gagtggatta cgcatgttga tcgaatacga tcagcggatg caggtcgtgg cgcaggctgg
    12241 cagcaaagtt gaagcgctcg aaagagccgc cgccaccaat ccggatgtga tcattttgga
    12301 tctgttgctc ggtgacgatg atggtctttc gttcctgccc gagctctgcc aggccagccc
    12361 gaattcacgt gtgctcgtgt tgacgggagt gcagaatgcg gacgcgcacc gccgtgcgat
    12421 tcgtcgtggc gcgatgggca tcgtgctgaa agaacacgct gcagataagc tgctgaaagc
    12481 gatcatgaag gtgagtgagg gcgaggtgtg gatcgagcgg tcgatgatgg gcagtatgat
    12541 ccaggagttt aataaacctg cgttggtgga ccctgaggtc tcgaagatcg aatcgcttac
    12601 cgatcgcgag cgcgaagtga ttgcgttgat tggtgaagga ctgaagaaca agcaggtcgg
    12661 cgagcgcttg tttatcagtg agacaacggt aactcatcat ctgagctcgg tgttctcgaa
    12721 gctggaagtt tccgatcgcc tggagctgat tatctacgcc ttccgccacg gcctcgccaa
    12781 aatgcctaag tagatccctc ccctcagaca ggcagaaagc tgccacataa attggcaatt
    12841 acctctattg tcataaaatt gcaacaacct ccataatccg tcccagagcc tcaacacctg
    12901 ccgttacgga tttgtcatgg agcctgcacc gatcaaaatc cgaatcttaa tagttgacga
    12961 tcagctagtg gtgcgtgagg gtcttcgtat gctgatcgag aaccatcccg gcaccaaagt
    13021 cattgcgatg gccagcactc gttcggaagc gctcgacatt ctcgcccgcg aaacgtgtga
    13081 cctgatcatt ctcgatctcg agctcggcgg ttatagcgcc acgtctttca tccctcaatt
    13141 gcgagaagcg gcaagaaatt cgcgcgtgct cgtgttgacg ggatcgcgcg ataccgcaat
    13201 tcatcaaaag gcggcgcaac tcggtgcgat gggtgtggtg ctgaaggaag acgctgccga
    13261 ccttttgctc aaagcgatcg agaaggtcta caaaggcgaa gcatggctcg atcgggtcac
    13321 gctggggaat ctgctgttcc agatgtcaaa ccacgacagg cagccactcg atccgcgcgc
    13381 gcagaagatt tcttcactca cggatcgtga gcgacaagtg atcgctttga tcgccgaggg
    13441 gctgaagaac cgtcagattg cggaacgact cttcatcagt cccaccaccg tcacccatca
    13501 cctcagctcc atctattcaa agctcggcgt ctccgatcgt ctcgagctcg tcatctacgc
    13561 cttcgccaac aaactcgcga agatgccgga ataaattccg aaacaagata agcaagctcg
    13621 ccaggacttc gaaaagctcg cgaagcgagc gacagcataa agcctggggc gccagcccca
    13681 ggctcacgaa cctaaattat tggtagagcc cgcggagcgg gcgacagcgc acgtgttcat
    13741 cggctgtcgc ccacttcgcg ggctcaattt gtttttgtta acgcgatcct ggggctggcg
    13801 ccccagaaac tatgaagccg ggtctcccga agtcgatttg aaagttggac aatgtccaac
    13861 gtttgagacg agtttggcct ttgcaggttg actcaaaatc tgactgagaa gggagtaccc
    13921 ggtgatgaa
//
LOCUS       DASSFQ010000197        13031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_987171, whole genome shotgun sequence.
ACCESSION   DASSFQ010000197 DASSFQ010000000
VERSION     DASSFQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987171"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFH96_06855"
     CDS             <1..444
                     /locus_tag="VFH96_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973728.1"
                     /translation="CGSGVTTILMGILCGKRNVDVWSLEHYDEWRQRVSGVLADNDIA
                     GATVCSAPLVEYGEFVWYDPPLAQMPEAFSLVICDGPPGSTKGGRYGLLPVLGDRLQE
                     ATILLDDAERPGEAELIERWENEAHFETEVRGGKERGFAVMRRLG"
     gene            449..2380
                     /gene="asnB"
                     /locus_tag="VFH96_06860"
     CDS             449..2380
                     /gene="asnB"
                     /locus_tag="VFH96_06860"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6973729.1"
                     /translation="MCGIAGFIDLWDSREAREKQERAAILDRMCRIIQHRGPDDQGVT
                     VRPGVALGMRRLAIIDLVSGNQPMSGEDGTVTIVFNGEIYNFQEIKPKLEARGHIFQT
                     HSDTEAIVHAYEEFGPDCLKDLRGMFAFAIWDDKARTLFVARDRAGKKPLYYTTTANG
                     TFVFGSELKALLEHPDVQRELNPEGLDAYFTLGYVPDPLSIFRHIHKLPPGCYLTFRN
                     GEVSVKQYWDFELEPAASTKEADYLDELRFLLDESVRLRLISDVPLGAFLSGGIDSST
                     VVALMARHMGQPVKTFSIGFHEDSYNELKFARVTAQKYGTDHHEFFVTPDICEVVDDL
                     AWHFDEPFADSSAIPTYMVSKLARDHVTVVLSGDGGDELFAGYTRYVVDRKRGGFERL
                     PKPLREGVMRPLSERLPHAAWGRNYLHNVSLDPISRYLDSVSVFTNLNRRSLYTSDFS
                     RQLGPGGYVARLFNKLVENVKSDEPLDRLLYLDSKTYLPGDILTKVDRMSMAVSLEAR
                     APLLDHKLIEFVTRVPAALKLAGLETKHLLKRAVRDLVPAEILDRPKQGFGVPIQEWI
                     NQQLRSRIRDTLSDARTKQRGYIDSHYVGVLLDEHERGRRDHSMSLWALVMLELWHRQ
                     FLDGPTPGRKELREAQLVS"
     gene            2392..3525
                     /locus_tag="VFH96_06865"
     CDS             2392..3525
                     /locus_tag="VFH96_06865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973730.1"
                     /translation="MKPAVLQLIDSFNQGGSERQALQLTRLLVESGRFKVRLACLSPE
                     GVLRDSIDDLNLGEIPSFPLNSFTDRNAVRQLKRFVRWLKSSRIDILHTHDFYTNIFG
                     MAGGGLARLPVRVASMRETAGMRSAAQKQVQRIAYSMAHHVVANSNAVRERLIGDGVA
                     PEKVSVIYNGLDIRRLATTLSRAESLSLLGLPERRFIAIIANMRHDVKDYPMFLRSAR
                     RVKDAVPDAAFLLAGEGELSDSLRVMAGELGIDDSAFFLGRCEKVAELLAVSEICVLS
                     SKAEGFSNSILEYMAAGRPVVVTDVGGAREVVAESETGHLVPSGDDAMMAERLIALLH
                     DPERARAMGAKGKRVVEEKFSCEAQLARTESLYDQLFIHRLHR"
     gene            3585..4721
                     /locus_tag="VFH96_06870"
     CDS             3585..4721
                     /locus_tag="VFH96_06870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6973731.1"
                     /translation="MNTQVQSPVRVLIVAPSFGILGGQSVQAARLLERLRQEPSLEVG
                     FLPINPRLPGGLHHLQGIKYVRTVVTSIAYGLSLLARVYKYDVIHVFSASYFSFVLAP
                     TPAILIGKLYRRKILLNYHSGEAEDHLQRWQRTAIPTIRLVDSIIVPSEYLVRVFASF
                     GLKATAIYNLIDTSTFRFRERSPLRPVFLSNRNLESHYGVDRVLRAFAIIQERIPHAS
                     LTVAGDGSQRQFLQTLAHELKLQNTQFVGQIAPAKFAEVYDAADIFLNGSEIDNQPLS
                     ILEAFSCGLPVVTTDAGGIPDIVQDGRTGMVVSRGDHVEIANRALALLENPVLAKQIV
                     DHARQECLKYSWEAVRDAWMSAYHGLANKGEQHAGSERALVDQR"
     gene            4718..6889
                     /locus_tag="VFH96_06875"
     CDS             4718..6889
                     /locus_tag="VFH96_06875"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET6973732.1"
                     /translation="MKSKLHKLRQVGFDELRVRGSQAFAAFAERQNWSNSSKLISDDA
                     LLRLLNGESKSPSDLLNHFRERTSPKFFASFADPQATVNELRRRWPGAENQIVKRADY
                     IVAGYFDLLGFRNLNFGNPIDWHLEPVAGKRAPLVHWSRLNYLDADLFGDKKITWELN
                     RHQYFITLGQAYWLTGNERYAKTFVEHVNSWMDQNPPKLGINWASSLEIAFRSIAWIW
                     ALHFFKDSDALTPETFSSLCKFLYLNARHLETFLSTYFSPNTHLTGEALGLFYLGALM
                     PEFKESARWRNRGLEILLGQLSKHVQPDGVYFEQSSYYHRYTTDFYLHLRVLLANTGA
                     PIPSVLDEKLCLLLDHLMYITRPDGTTPHFGDDDGGRLLSFFAAPANDFRATLAAGAA
                     LFERADYRFVAGDALEDALWLLGPASFDQIVATEPEKQSVAFPAGGYYVMRDGWTAKS
                     NYLLFDCGPHGTDNCGHAHADALSFELAVNGKTVLVDPGTYTYTGSKEMRDWFRSSEA
                     HNTLTIDGESSSVSEGPFSWKTIARSECSSWITEEGFDYIVASHDGFMRLSEPAKHTR
                     SILFIKNRYWVVRDVVETSGDHVLQARFHLDPRVTLLQSEDNTVRAFSENSDAPVLQF
                     SAFAKNIEWETEHAPVSECYGEKKEALVLSISVLAKGSEELVVFLLPDGAVVREIEAQ
                     DGRTFEIEIGGRRDRLTLRDGENPPQIYTDEHGSVCAVLTE"
     gene            6868..8805
                     /gene="asnB"
                     /locus_tag="VFH96_06880"
     CDS             6868..8805
                     /gene="asnB"
                     /locus_tag="VFH96_06880"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6973733.1"
                     /translation="MCGINGIALASRAVDQRVLERMRDVITHRGPDEAGIFVDANIGL
                     GHRRLSIIDVASGHQPMTNEDGSLQIIYNGEVYNHADYRDELEARGHVYRTHCDTETI
                     LHLYEEHGEACVNYLRGMFAFAIWDGKKKELFIARDRLGVKPLYYFHADDGSLYFGSE
                     IKTIFQASDLKPEINFHALPDYLANHATSGEETLYHRVKRLLPGHTLLWRDGKIEIKK
                     YWDVSFAKTADASRSDKDFIAEWYELFRTSVRLRLMADVPLGMFLSGGIDSSAIAAVM
                     SGMVAEPIKTFSVAFAEREANELAYARIVAEAYKTNHHEVVVSPEEFFNALPRLVWHE
                     DEPLAHPSSVALYFVSLLASQHVKVVLTGEGSDELLAGYGRYRKTILNLSLGKRYWSL
                     TPDVVRGAVSSGIKGLPTSRMRQKLLRSFLGVAPDIESIYFDNFAVFPLAMQADLLSN
                     EAVEQIGRIDPYAGVRTALAETDAESLLDRLLYTDIKTYLHELLMKQDQMSMATSVES
                     RVPFLDHKLVEFTCSLPERLKLRGGTTKYILRESMKGVLPEAILSRSKMGFPVPIASW
                     FRGAYRSVIDEYVLSERAMSRGLFNRDFVRELVNRHQSGVENHDERLWALVNFEIWQR
                     QFFDGEAAPETLRLREETILI"
     gene            8883..10151
                     /locus_tag="VFH96_06885"
     CDS             8883..10151
                     /locus_tag="VFH96_06885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973734.1"
                     /translation="MRILWLKTELLHPVDKGGKIRTYNMLKELKRDHFISYMSLDDGS
                     ADSAAREQASEYCNELICVPYQQREKFSSGFYAELLLNLASRFPYAIRKYESASMRSQ
                     ISERVNSGTFDVVICDFLAPAINVPRDLSCPTILFQHNVEAMIWKRHFEVQNNQLKKI
                     YLRQQWRKMERFERETCRRFDTVIAVSKDDCAQMRNEYAVDNVFEVPTGVDTDFFTPS
                     GKEVVDPRNVVFTGSMDWLPNEDAIRYYTDQILPLIRRSLPDVTLTVVGRNPYPGLIE
                     LSEKDPGIVVTGRVEDVRPYMERAAAYVVPLRIGGGTRLKIYEAMAMEKAIVSTTVGA
                     EGLPVTDGKELFIADAPEKFAAAVVKLLTNPSQANEVGRQARQTVVEKFGWSGVAKCF
                     AEICETTIRDHRRTRVATSVDVRSQQQSAV"
     gene            10069..11184
                     /locus_tag="VFH96_06890"
     CDS             10069..11184
                     /locus_tag="VFH96_06890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6973735.1"
                     /translation="MRRRFAIIVAPESQPVLMLEANNNQPFEQPMSIRAVEACDSVSV
                     IIPCYNEERFIGKALEQLADQFESDRYELVIVDGSSEDRSREVVDEFRTRRPDVPVLL
                     VDNPARNIPTALNLGIAAARGNIIARMDAHAVPSDGYIRRCVEVLKTGNAGAVGMPCL
                     VRPGATTLIAQGIAAAVSHPFGIGDAKYRLGARGPQQEAVDTVAFACFRKSLWKELGG
                     YNESLHTNEDYDFNYRVRRSGRQVILDRGGHCDYFARSTLRTLASQYVRYGGWKAEMI
                     RLHPRSTKLRHLVAPLFVASIVALGIAGIFWKLAWWLLLLELVTYLGCALLAGWQAAR
                     RAGAGLQMALVMPVIFATIHLSWGWSFLVRLIKPAGK"
     gene            11188..12498
                     /locus_tag="VFH96_06895"
     CDS             11188..12498
                     /locus_tag="VFH96_06895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET6973736.1"
                     /translation="MKLSIFGLGYVGCVSAACFAKEGHTVTGVDVNANKAEMINSGTS
                     PIIEAGIGPLLDEVVKANRLSATTDSAAAVHNTDVSLICVGTPSNTNGSLDLRYVTRV
                     CEEIGAALKDKTERHIVVIRSTMLPGTIESVVIPTLEEHSTKRVGKDFGVCINPEFLR
                     EGSSLKDFYAPPFTLIGADDRQTTATVRELYSRIDAPVIATSVKTAEMVKYVCNCFHA
                     LKVSFANEIGNICKAVGVDSHEVMDIFCQDTKLNLSPYYLKPGFAFGGSCLPKDLRAM
                     NYKAKQVDVEVPVLSSILPSNKRQIEHAIDMVLATGRKRVGVVGFSFKAGTDDLRESP
                     IVTLIETLLGKGIQLVVYDKEVSLARLFGANKEYIERQIPHIAQLMRSSIDEVLEFAE
                     VLVIGNNAEEFRQIEQKQREGQVVIDLVRLFNKTSDETYQGICW"
     gene            12495..>13031
                     /locus_tag="VFH96_06900"
     CDS             12495..>13031
                     /locus_tag="VFH96_06900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET6973737.1"
                     /translation="MNPLVSVIIPAYKVAPYLAETLESVFAQTFSDFEVILVNDGSPD
                     TDEMEKVIEPYRDRIVYLRQENRGAGAARNTGLRSARGRYVAFLDGDDIWLPGFLSEM
                     VSFIDGYDLVYANALLFGASQVAGLTYMDTNPSSGEVTCESLLAERCNIITSGVLARR
                     EPIVELGMFDETLRNSQDF"
BASE COUNT         2836 a         3374 c         3809 g         3012 t
ORIGIN      
        1 tgcggcagtg gtgtgacgac gattctgatg ggaattttgt gtggcaaacg caacgtcgac
       61 gtgtggtcac tcgaacatta tgacgaatgg cggcagcgtg tctcaggcgt cctcgcagac
      121 aatgatattg cgggcgccac cgtttgctcg gcgccgttgg tagagtacgg agaattcgtc
      181 tggtacgatc ctccgctggc gcaaatgccc gaagcgttct ccctcgtgat ttgcgacgga
      241 cctccgggat cgacaaaagg cggccgttac ggactgctgc cagtgttggg cgatcggctg
      301 caggaggcga cgattttgct tgacgatgcc gaacgtcccg gcgaagcgga gttgatcgag
      361 cgttgggaaa acgaggcgca ttttgagact gaggttcggg gtggcaaaga gcgtgggttc
      421 gcggtgatgc gacggttagg ttgattacat gtgtggcata gcaggattta tagatctctg
      481 ggactcgcgt gaggcgcgcg aaaagcagga gcgtgcggcg atactcgatc gcatgtgtcg
      541 catcatccag catcgcggtc cggacgatca gggcgtgacg gttaggccgg gcgtggcgct
      601 gggcatgcga cgcttagcca tcatcgatct tgtcagcggc aaccagccga tgtctggcga
      661 agacggcaca gtcacgatcg tcttcaacgg cgaaatctac aacttccagg aaatcaagcc
      721 gaagctcgaa gcgcgtggcc acatctttca aacacactcc gacaccgaag cgatcgtcca
      781 tgcttacgaa gagtttggtc ctgactgtct gaaagatctg cgcggcatgt ttgcctttgc
      841 gatctgggac gacaaagcgc gcacgctctt cgtcgcgcgc gatcgcgccg ggaaaaaacc
      901 cctctattac acgaccaccg caaatggaac attcgttttc ggctcggagc tgaaagccct
      961 cctcgaacat cctgacgtcc agcgcgagct caacccggaa ggactcgatg cttacttcac
     1021 gttgggttac gttccggatc cgctgagtat ctttcgtcac attcacaagc tgccgccggg
     1081 atgctacctg acttttcgaa acggcgaagt ctcggtgaag cagtactggg atttcgagct
     1141 cgagcccgcc gcatcaacga aagaagcaga ctatctcgac gagttacgtt ttctgctcga
     1201 cgaatcagtt cgtttgcgtt tgatttccga tgtgccactc ggcgcgtttc tctccggtgg
     1261 tatcgattcg agcacagtcg ttgctttgat ggcgcgacac atgggccaac ctgtgaagac
     1321 gttctcgatc ggctttcacg aagacagcta caacgaattg aagtttgcgc gggtgacggc
     1381 gcagaaatac ggcacggacc atcacgagtt ttttgtcacc ccggacatct gcgaggtagt
     1441 cgacgatctc gcgtggcatt ttgacgagcc gtttgccgac tcgtccgcta ttcccaccta
     1501 catggtttcg aaactcgcac gcgatcacgt caccgtggtg ctgtccggcg atggtggtga
     1561 cgagttgttt gcgggttaca cgcgttatgt cgtcgatcgg aaacgcggag gctttgagcg
     1621 tttgccgaag ccgctgcgcg aaggagtgat gcggcctttg agcgagcgac tgccgcacgc
     1681 cgcgtgggga cgcaactacc tgcacaacgt ttcgctcgat ccgatttcac gatatctcga
     1741 cagtgtttct gttttcacga acctgaatcg ccgatcgctt tacacgtcag acttttcgcg
     1801 gcagcttgga ccaggcgggt acgttgccag gcttttcaat aaactggtcg agaacgtcaa
     1861 aagcgatgag ccgctcgatc gtctgctgta tctcgacagt aaaacctatc tgcccggcga
     1921 tatactcacg aaagtcgatc gcatgagtat ggcggtatca ctcgaagcac gagcgccgtt
     1981 gctcgatcac aagctgatag agtttgtcac acgcgttccc gcggccttga agctggccgg
     2041 gctcgagacc aaacacctgc tcaagcgagc tgtaagagac ctcgttccgg cggagattct
     2101 cgatcggccg aagcagggat ttggtgtgcc gattcaggag tggatcaatc aacaattgcg
     2161 ctcgcgtatt cgtgatactc tgagcgatgc gcggacgaag cagcgaggct acattgattc
     2221 gcattacgtc ggcgtgttgc tggacgagca cgagcgcggc cgtcgcgatc attcaatgag
     2281 tttgtgggct ttggtgatgc tcgagctctg gcaccggcag tttttggatg gtccaacacc
     2341 cggccgcaaa gagttgcgcg aagcacagtt ggtaagctga ccacgtacaa agtgaagcct
     2401 gccgttcttc aattaatcga cagctttaac cagggcggat ccgagcgtca ggctttgcaa
     2461 ctcacgcggc tgctcgttga aagcggccgg ttcaaagttc ggcttgcgtg tctcagtccg
     2521 gaaggtgtcc tgcgcgatag tatcgacgac ctcaacctcg gcgagattcc ttcctttcct
     2581 ttgaatagct tcaccgatcg aaacgctgtc agacaactca aacgttttgt gcgctggttg
     2641 aagtcgtctc gcatcgacat tcttcataca catgatttct atacaaatat ttttggcatg
     2701 gccggtggcg gactcgcgcg tttgccggtg cgcgtcgcgt cgatgagaga gactgcgggg
     2761 atgcgtagtg cggcgcagaa gcaggtgcag cgaatcgcgt actcgatggc gcatcacgtc
     2821 gttgccaatt cgaatgcggt gcgcgagagg ctcatcggcg atggtgtcgc gccggaaaag
     2881 gtgtcggtca tctacaatgg tttggacatt cggcgactgg ccacgacgtt gtcgcgagcg
     2941 gagtcgcttt cgctgctggg gctaccggag cgccggttca tcgcgatcat tgcgaacatg
     3001 cggcatgacg ttaaggacta tccgatgttt ctgagatcgg cgcgacgggt gaaagatgca
     3061 gttcctgatg cggcgttctt gctggccggt gagggtgagt tgagcgattc gttgcgtgtg
     3121 atggctggag agctgggtat cgacgactcg gcgttctttc tcgggcgttg tgaaaaggtg
     3181 gctgagctgc tggctgtttc ggaaatttgt gtgttgagct cgaaagcaga agggttttca
     3241 aactctattc ttgagtatat ggctgcgggg cggcccgtgg ttgtaactga tgttggtggt
     3301 gcgcgagagg tcgttgctga aagtgagacg ggtcacctcg tgccttcggg tgatgatgcg
     3361 atgatggcgg aacgattgat cgcgctgctc cacgatccgg agcgcgcccg agcgatgggc
     3421 gcaaaaggca agcgcgtggt cgaagagaaa ttctcgtgcg aagctcaact ggcacgcacc
     3481 gagagtcttt atgatcaatt attcatccac agattacaca gataacacag attgactcaa
     3541 aataatctgt gtcgtctgtg taatctgtgg atgaacaaat gcaaatgaac actcaagttc
     3601 aatcgccggt gcgtgtgctg attgtggcgc cgtcgttcgg aattctcggc gggcagtcgg
     3661 tgcaggcggc gcgtttgctc gagcgtctca ggcaggagcc ttcgttggag gtcgggtttc
     3721 tgccgatcaa tccgcgtctg cccggtggtc tacatcatct gcaaggaatc aagtacgttc
     3781 gcaccgtcgt cacgtcgatc gcttacgggt taagcctgct cgctcgggtt tacaaatacg
     3841 atgtcattca cgttttctcc gcttcgtact tctccttcgt gctggcccca acgcccgcga
     3901 tcctgatcgg caagctctat cgccgcaaaa tactgctcaa ctatcacagc ggcgaagcgg
     3961 aagatcatct acaacgctgg caacgcaccg ccattcccac gattcgcctc gttgattcaa
     4021 taatcgttcc ctcggaatac ctcgttcgcg tcttcgcttc cttcggacta aaagcaactg
     4081 cgatctacaa cctgatcgac acgagcacat ttcgctttcg cgagcgctcg ccattgcgcc
     4141 cggtgttttt atcgaatcga aacctggaga gccattacgg cgtcgaccgc gtgctgcgcg
     4201 cgtttgcgat cattcaggaa agaattcccc acgcgagtct gactgtcgcc ggtgacggca
     4261 gccagcgcca atttctccaa acgctggccc acgaactcaa gctgcaaaac actcagttcg
     4321 tcggtcagat cgctcccgca aagttcgccg aggtttacga cgccgcagac atcttcctga
     4381 acggctccga gatcgacaat caaccactct caatcctcga agcgttttca tgcggcttgc
     4441 cggttgtaac aacagacgcc ggtggcattc cggacatcgt gcaggacgga agaactggca
     4501 tggtcgtgtc tcgtggcgat cacgtcgaga ttgcgaatcg agccctcgcg ttgctggaaa
     4561 atcctgtgct cgcaaaacag atcgtcgatc acgcgcgaca ggagtgtttg aaatacagtt
     4621 gggaagcagt gagagacgca tggatgagcg cctatcatgg tcttgcgaat aagggcgagc
     4681 aacacgcagg cagcgaacga gcgctcgtag atcagcgatg aagagcaagt tgcacaagct
     4741 tagacaggtt ggtttcgacg agctgcgcgt ccgcggctcg caggctttcg ctgcgtttgc
     4801 tgaacgacag aactggtcca atagctcgaa gctgatttct gacgatgcgt tgctgcgctt
     4861 gctcaacggt gaatcgaaat caccttcgga tctgctcaat cactttcgcg agcgaacgag
     4921 tccgaagttt ttcgcgtcat tcgccgatcc gcaagccact gtcaacgagc tcagacgtcg
     4981 ctggcctggt gctgaaaacc agattgtgaa acgcgcggac tacatcgtag ccggctattt
     5041 cgatctgctc ggctttcgta atctcaactt tggcaatccg attgactggc atctcgagcc
     5101 cgtcgcaggc aaacgtgcgc ccctcgtcca ctggagccgt ttgaactacc tggatgccga
     5161 tcttttcggc gacaagaaga tcacctggga attaaaccgg caccagtatt tcatcacgct
     5221 cggccaggct tactggctca cgggcaacga gcgctacgca aaaactttcg tcgaacacgt
     5281 taactcgtgg atggaccaga acccgccaaa gcttggcatc aattgggcca gcagtctgga
     5341 aatcgcgttt cgatcgatcg cgtggatctg ggcgcttcat ttcttcaaag attctgacgc
     5401 gctcacgccg gagacgtttt cgagtttgtg caagtttctg tatctgaacg cacgacacct
     5461 cgagacattt ctttccactt acttcagtcc caacacgcac ctgaccggcg aggcgctcgg
     5521 gctcttctat ctcggggcgt tgatgcctga gttcaaagag tctgcaagat ggcggaacag
     5581 aggtcttgaa attctactgg ggcagttgtc gaagcacgtg caaccggacg gcgtctattt
     5641 tgagcagtcg agttactacc atcgctacac gacggacttt tacctgcacc tgcgcgtgtt
     5701 gcttgccaac actggcgcac cgataccttc agtgctcgat gaaaagctct gcttgctgtt
     5761 ggaccatctg atgtacatca cgcgaccgga tggcacgacg ccgcactttg gagacgacga
     5821 cggcgggcgt ctgctgagtt tcttcgctgc acctgccaac gactttcgcg ccaccctcgc
     5881 cgccggtgcc gcgctgtttg aacgcgctga ttacaggttt gtcgccggtg acgcgcttga
     5941 agacgcactc tggttacttg gaccagcctc tttcgatcag atcgttgcaa ccgaaccgga
     6001 aaagcagtcg gtcgcgttcc cggcaggtgg ttattacgtc atgcgcgatg gctggactgc
     6061 taaatcgaat tacctgctgt tcgattgcgg acctcatggc acagacaact gtggccacgc
     6121 acatgcagac gcgctctcgt ttgagctagc cgttaatggc aaaaccgtgc tggtcgatcc
     6181 gggaacttac acgtacaccg gctcgaaaga gatgcgcgat tggtttcgca gctctgaagc
     6241 gcacaacacg ctcacgatcg atggtgagtc gtcatcggtg tctgagggtc cgttctcatg
     6301 gaagacgatt gcgcgttccg agtgctcttc gtggattaca gaagagggtt ttgactacat
     6361 cgtcgcatcg cacgatggtt tcatgcgtct ctctgagccc gccaaacaca cgcgctcgat
     6421 cctgttcatt aaaaacagat actgggtcgt gcgcgacgtc gtcgaaactt ccggcgacca
     6481 cgtgctacag gcgcgatttc atctcgatcc gcgggtgacg ttgttgcaaa gcgaggacaa
     6541 cacggtgcgc gcattcagtg aaaatagcga cgcgccagtc ctgcaattca gcgcttttgc
     6601 taagaatatc gagtgggaga cggaacatgc tcctgtttcg gagtgttatg gtgagaagaa
     6661 ggaggctctc gtcctctcga tcagcgttct ggccaagggt tcggaagagc tagtagtatt
     6721 tcttttgcct gatggcgcgg tcgttagaga gatcgaggcg caggacgggc gcacttttga
     6781 aatcgagatt ggcggccgac gcgatcgcct gacgcttcga gacggagaga atccgccaca
     6841 gatttacacg gacgaacacg gatcagtatg tgcggtatta acggaatagc cctggcatcg
     6901 cgcgctgtcg accaacgtgt cctcgagcgc atgcgcgacg tgatcacgca tcgcggacct
     6961 gacgaagcgg gaatcttcgt cgatgccaat atcggtctcg gtcatcgccg tttgagcatt
     7021 atcgacgtgg cctcaggtca tcaaccgatg actaacgaag acggttcact tcagatcatc
     7081 tacaacggcg aggtctataa ccacgccgat tatcgcgacg agctggaagc gcgcgggcac
     7141 gtctatcgca cccactgcga cacggaaacg attcttcatc tctacgaaga gcacggcgaa
     7201 gcgtgcgtca attacctgcg cggcatgttt gccttcgcca tctgggacgg gaagaaaaaa
     7261 gagctcttca tcgcgcgcga tcgactcggg gttaagccgc tctattactt tcacgctgac
     7321 gatggctcgc tctacttcgg ctcagaaatc aaaacgatct tccaggcgag cgatttaaaa
     7381 cccgagatca actttcacgc gctgccggat tatctcgcca atcacgcgac ttcaggtgaa
     7441 gaaactctct accaccgcgt caagcgtcta ttgccgggcc acacacttct atggcgcgac
     7501 ggcaagatcg agatcaagaa gtattgggac gtgagtttcg cgaagactgc tgacgcgtcg
     7561 cgcagtgaca aagacttcat cgccgagtgg tacgagctgt ttcgcacttc ggtgcggctg
     7621 cgattgatgg ctgatgtgcc gctgggaatg tttctgtcag gcgggatcga ctcgtcagcg
     7681 atcgcagcgg tcatgagcgg catggtcgct gagccgatca agacgttctc ggtcgcgttt
     7741 gccgaacgag aagcaaacga gctcgcctac gcgcgaatcg tggctgaagc ttataagaca
     7801 aaccatcacg aagtggttgt cagtccggaa gagtttttca atgcgttgcc gagactcgtt
     7861 tggcatgagg acgagccgct ggcccatccg tcgagtgtgg cgctctattt tgtttctctg
     7921 ctggcgtcgc agcacgtgaa agtcgtgttg acgggcgagg gaagcgacga gctgctggcc
     7981 ggttacggac gttatcgcaa gacgattctt aatctctcgc tcggcaagcg ttactggagt
     8041 ttgacgccgg atgtggttcg cggtgccgtg agtagcggga tcaaaggctt gccgacgtcg
     8101 cgcatgagac agaagctgct gcgcagtttt ctcggtgtcg cgcctgatat cgagagtatc
     8161 tacttcgaca acttcgcggt gtttccgctc gcgatgcagg cagacttgct ctctaatgag
     8221 gcggtggagc aaatcggtcg catcgatcca tacgccggcg ttcgcactgc gctcgccgaa
     8281 actgatgctg agtcgttgct cgaccgcttg ctctatacag atatcaagac ttacctgcat
     8341 gagttattga tgaagcagga ccagatgagc atggcgactt cggtggagag ccgtgttccg
     8401 tttctggatc ataagctggt cgagtttacg tgcagtttgc ccgagcgatt gaagctgcgt
     8461 ggcggtacga cgaaatacat cttgcgcgag agcatgaagg gtgttttgcc ggaagcgatc
     8521 ctgtcgcgat cgaagatggg atttccggtg ccgattgctt cgtggtttcg cggtgcttat
     8581 cgctcggtga ttgatgagta tgtgttgagc gagcgtgcga tgagtcgcgg gttatttaat
     8641 cgcgactttg tgcgcgagct cgtgaatcga catcaatccg gtgttgagaa tcacgatgag
     8701 cgactatggg cactcgtcaa ttttgagatc tggcagcggc agttttttga cggcgaagcg
     8761 gcgccggaaa ctttgcgtct acgggaagaa acaatcctta tttagccgcg gatctacgcg
     8821 gatgaacgca gatctatccg cgtttatctg cgtaaatccg cggccaaaaa actcgataaa
     8881 tagtgcggat tctttggcta aaaactgagc tgcttcatcc ggttgataag ggcgggaaga
     8941 ttcgcactta caacatgctg aaagagttga agcgcgatca tttcatctcg tatatgtcgc
     9001 tcgacgacgg ttcggcggat agcgccgcgc gcgagcaggc ttcggaatat tgtaacgagc
     9061 tgatttgcgt gccttatcaa cagcgcgaga aattttcgag cgggttttat gctgagctcc
     9121 tgcttaacct cgcgtctcgc tttccttacg caatcaggaa atacgaatcg gcttcgatgc
     9181 gcagccagat tagcgagcgt gtgaacagcg gcacgttcga cgtcgtcatc tgcgactttc
     9241 tagcgccggc gatcaacgta ccgcgcgatt tgtcgtgccc cacgattctc ttccagcaca
     9301 acgtcgaagc gatgatctgg aaacgacact tcgaagtaca aaacaatcaa ctcaaaaaaa
     9361 tctatctgcg acaacagtgg cgcaagatgg agcgctttga aagagaaacg tgccggcgct
     9421 tcgataccgt gatcgcagta tcaaaagacg attgcgcgca aatgcgaaac gaatacgcag
     9481 tcgataacgt ctttgaagtg ccgaccggtg tcgacacaga tttcttcaca ccgtccggca
     9541 aagaagtggt cgatccacgt aacgttgtat tcaccggatc gatggactgg ctcccgaacg
     9601 aagatgcgat tcgttactac acggatcaga tactgccgct gatcaggaga tcgcttccgg
     9661 acgtcacgct gaccgtcgtg ggccgcaatc cttatccggg tctgatcgag ttgagcgaaa
     9721 aagatccggg aattgtagtc acgggccgcg tggaagatgt tagaccatac atggagcgcg
     9781 cagcggcgta tgtcgtaccg cttcgtatcg gcggcggtac gaggttgaag atttacgaag
     9841 caatggcaat ggagaaggcg atcgtctcca cgaccgtagg cgctgaaggt ttgccggtga
     9901 cagacggaaa agagctgttt atcgccgacg cgccggagaa gtttgccgcc gcggtggtga
     9961 aactactaac caatccgtca caggcaaacg aggtgggccg gcaggcgcga caaacagtgg
    10021 tcgagaagtt tggctggagt ggcgtggcta agtgcttcgc ggaaatttgt gagacgacga
    10081 ttcgcgatca tcgtcgcacc agagtcgcaa ccagtgttga tgttagaagc caacaacaat
    10141 cagccgtttg agcagccgat gtcgatacga gcagtagaag cctgcgatag cgtgtcagtc
    10201 atcatcccct gctacaacga agagcgtttc atcggcaagg cgctcgaaca gctcgcggac
    10261 cagtttgagt ccgatcgata cgagctcgtc attgttgacg gttcgtcgga agatcgcagt
    10321 cgcgaagtgg ttgatgaatt cagaacacgc cggcccgatg tgcctgtgtt gcttgtcgat
    10381 aatcccgctc gcaacattcc gactgcactc aatctcggca tcgccgcagc aagaggaaac
    10441 ataatcgcgc ggatggacgc gcacgccgtg ccgtcagacg gatacatccg ccgctgcgtt
    10501 gaagtgttga aaacaggtaa tgcgggagcg gtgggaatgc cgtgtctcgt gcgaccaggc
    10561 gcgaccactt tgatcgcgca gggaatcgcc gcagccgtct cgcacccttt cggaatcggc
    10621 gacgcgaaat accgtctggg cgcgagaggc ccacagcagg aagccgtcga cacagtcgct
    10681 ttcgcctgct ttcggaagtc actctggaaa gagctcggcg gctacaacga gtcgctgcat
    10741 acaaacgagg attacgattt caactatcgg gtgcgacgct ccgggcggca ggtaattctg
    10801 gatcgcggcg gccattgcga ttattttgca cgttcgacgc tgagaaccct ggcctcgcag
    10861 tacgtgcgat acggcggctg gaaggctgag atgattcgac tccacccgcg ctcgacgaag
    10921 cttcgtcacc tcgtggcgcc gctttttgtc gcttcgatcg tggcgctcgg catcgccggg
    10981 attttttgga aactcgcgtg gtggttattg ctcctcgagc tcgtcacgta tcttggttgc
    11041 gcattgctgg cgggatggca agcggcacga cgcgccggcg caggtttgca aatggctttg
    11101 gtgatgcccg taatctttgc gactatacat ctgtcgtggg gatggagttt tttagttcga
    11161 ttaataaaac cagcaggaaa ataattcgtg aaactcagta tttttggtct tggctatgtc
    11221 ggttgcgtct ccgccgcgtg cttcgcgaaa gaaggacaca cggttacggg cgtagatgtg
    11281 aacgcgaaca aagccgagat gatcaactcg ggcaccagtc cgatcatcga ggccggtatt
    11341 gggccattgc ttgacgaagt cgtgaaagcg aatcgcttat cggcgacgac tgattcagcg
    11401 gcggcagttc acaacacaga cgtttcgctc atttgcgtgg gtacgccgag caatacaaac
    11461 ggtagtctcg acctgcgcta cgtgacgcgt gtttgcgagg agattggcgc cgcgttgaaa
    11521 gacaagactg agcgtcacat tgtcgtcatt cgcagcacga tgctgccggg gacaatcgag
    11581 agcgtcgtga ttccaacgct cgaagaacac tcgaccaaac gtgtgggaaa ggattttggc
    11641 gtctgtatca atcccgaatt cctgcgtgaa ggatcttcgt tgaaagattt ctatgcgccg
    11701 ccgttcacgt tgatcggagc agacgacagg cagacaacgg ctacagtgcg cgagctgtat
    11761 agccgtatcg acgcgccagt gatcgcaacc tctgtgaaga ctgcggagat ggtcaagtac
    11821 gtatgcaatt gttttcacgc gctgaaggtc agctttgcca atgaaattgg aaatatctgc
    11881 aaggcggtcg gcgttgacag tcatgaagtc atggatattt tttgccagga cacgaaactc
    11941 aatctatcgc cttactacct gaagcccggc tttgcgttcg gtggctcgtg tttgccgaaa
    12001 gacttgcgcg cgatgaatta caaggcgaaa caggttgatg tggaagtgcc ggttttatct
    12061 tcgatcctgc caagcaataa gagacagatc gaacacgcca tcgacatggt gttggcgacg
    12121 gggcggaagc gagttggggt agttggtttc agcttcaaag ccggtactga tgacctgcgt
    12181 gagtcgccca ttgtcacgct gatcgagacg ctgctcggaa aaggtattca gcttgtcgtt
    12241 tatgataaag aggtctcgct ggctcgtttg tttggcgcga acaaggaata catcgagcgg
    12301 cagattccgc atatcgctca gttaatgcgt tcgagtattg atgaggtact cgagtttgct
    12361 gaagttttgg ttatcggtaa caacgctgaa gagtttcggc agatcgaaca gaagcagcgt
    12421 gaaggacaag tcgtcatcga tctggtgcgg cttttcaaca agacgtctga cgagacgtat
    12481 cagggcattt gctggtgaac ccgttagtaa gcgtcatcat tccggcttac aaagtcgcgc
    12541 catatttggc ggagactttg gaatctgttt ttgcgcaaac tttttcggat ttcgaagtga
    12601 tacttgtcaa cgacggctcg cctgatacgg acgagatgga gaaggtaata gagccgtacc
    12661 gagacagaat tgtgtatctt cgacaggaga atcgcggcgc gggagcggca cgcaatacgg
    12721 ggctgcgctc ggcgcgcggt cgctacgttg ctttcctcga cggagacgac atctggttgc
    12781 cgggtttcct ttcggagatg gtgagtttta tcgacggtta cgatcttgtt tacgccaacg
    12841 ctcttttgtt tggtgcgtcg caggtagccg ggcttaccta catggatacg aatccatcga
    12901 gcggagaggt gacgtgcgag agcctgttgg ccgagcggtg caatatcatc acgtcgggcg
    12961 tgttggcccg gagagaaccg attgttgagc tcgggatgtt tgatgagacg ctgcgtaact
    13021 cgcaggactt t
//
LOCUS       DASSFQ010000198         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1865427, whole genome shotgun sequence.
ACCESSION   DASSFQ010000198 DASSFQ010000000
VERSION     DASSFQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865427"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFH96_06905"
     CDS             complement(<1..1026)
                     /locus_tag="VFH96_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET6973738.1"
                     /translation="MTGNEIREAFLRYFEEHGHTRVRSSPLLPANDPTLLFTNAGMNQ
                     FKDVFLGAEKREYVRAASSQKCIRAGGKHNDLDEVGKTARHHTFFEMLGNFSFGDYFK
                     EDAIKFAWELLVDELKLDPKRLWFTVYEGDAEVPPDEDAERFWEQVGAPRERILRFGR
                     KDNFWQMGETGPCGPCSEVHYYMGESPDDPENSAANVNGPGDTIMEIWNLVFMQFDRS
                     EAEGGKYKLDPLPKPSVDTGAGLERLAVVLQGVKSNYDTDLIRPIVEFTAKLADRHYE
                     PETQEGFAMRVIADHARATAFAIADGIVPGNEGRNYVLRKIMRRAIYQGHHTLRFEGP
                     FFNEVTDF"
     gene            complement(1048..1524)
                     /locus_tag="VFH96_06910"
     CDS             complement(1048..1524)
                     /locus_tag="VFH96_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET6973739.1"
                     /translation="MRRKTFERAIKLLTARPRSVGELRERLLNRKDTNEEVVEAVIAR
                     LREYGYLNDERFAFSYASYKVKQKPVGRRRLEQDLKFKKVDSSVADEALDMVFCETPE
                     EQLIDEAIAKRLRIRGKPKNRLEAKSLFEHLVRQGFEFELVRERVRSLANDYTDED"
     gene            1577..2515
                     /gene="truB"
                     /locus_tag="VFH96_06915"
     CDS             1577..2515
                     /gene="truB"
                     /locus_tag="VFH96_06915"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET6973740.1"
                     /translation="MSDMDGVLVIDKPSGMTSHDIVAQTRRILHERRIGHTGTLDPFA
                     TGVLVVLLGKATRLSQFMTGVDKQYEAIIRLGYSTDTGDRTGNPIPGPSTEAHNWTEP
                     EIEAALQSLRGNIDQVPPMYSAKKVEGKKLYELARRGETVERKPVRVCIHEFAAIRPE
                     GQLIKDNLDGTFDFHARVSCSSGTYVRTLAEDFGKRLYVGAHLAELRRTRVGDLRVDQ
                     AITLDELKIHFGEDSIGEVLLPPGTALSWLPSLHLSGEDVRRANHGRELKALERDWAD
                     GENVRIYDVGEELIAIGRYDASARSVHPQVVLSGEN"
     gene            2795..4732
                     /locus_tag="VFH96_06920"
     CDS             2795..4732
                     /locus_tag="VFH96_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HET6973741.1"
                     /translation="MQEQDGKKQFNISERLEDLRREHEALRWEGTFRDYFELVAQNPR
                     VAQLSHARINDMVHAAGIERHNEGTQHEVLSYNFFAHELFGIEEPIARIVEYFKSAAQ
                     RLEVRKRILLLMGPVGGGKSTIVNMLKRGLEDWTKTDAGAVYAIKDCPMHEEPLHLIP
                     HQLRAEVEKHYGIYIEGDLCPQCRYALEHTYGGRHEDVKVHRVGFSEKERVGIGTFSP
                     SDPKSQDITELTGSIDLSTIGDVGVESDPRAYRFDGELNIANRGLMEFVEMLKVDEKF
                     LYSLLTLSQEQSIKTGRFAMIYADEVILSHTNENEYIAFAGNRKSEALQDRIILVRVS
                     YNLRVSEEERIYDKLLHQSEALRNVHLAPHTLRVAAMFAVMTRLEEPKRQNVDIVKKM
                     KLYDGEDVEGYKSKDVRELKEETIREGMDGISPRYIINRLSSALVRDGVTCINPIDAL
                     RAIKDGFEQHTGISSEQRERYLNHISQARKEYDELAKIEVQRAFVYSFEEMARTMCNN
                     YLDNVEAFCNKERIKDPITEEEMDPDEQLMRSIEEQIGISENAKNTFRQEILIRISSH
                     ARKGKAFEYNSHERLKEAIEKKIFADLKDVVKITTSTKTPDPDQLRRINEVIDRLVKE
                     HGYCPVCANELLTYVGTLLSR"
     gene            4813..>5340
                     /locus_tag="VFH96_06925"
     CDS             4813..>5340
                     /locus_tag="VFH96_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6973742.1"
                     /translation="MKKRYWIAGAATLAIAGKLLTRPRDADWQKCRDVIFHSEHSCFI
                     DVDGIRVHYQEAGDPKAPALVLIHGFASSTLVWSKVFLRLAEAGYRVIALDLLGYGYS
                     AKPKNGEYTIAGQAKLLTRLLDALGIPRAIVVGSSYGGAVAATCALDYPDRVEKLILV
                     GAVNNNQPCDFMLMRF"
BASE COUNT         1253 a         1421 c         1496 g         1170 t
ORIGIN      
        1 gaagtcagta acctcattga agaacgggcc ttcgaatcgc agcgtgtgat ggccctggta
       61 gatcgcgcgt cgcatgatct tgcgtaacac gtaattgcga ccttcgttgc cgggaacaat
      121 tccgtccgca atcgcgaagg cagtcgcgcg cgcgtggtct gcaatgacgc gcatcgcaaa
      181 accttcctgc gtttccggct cgtagtggcg atcggcgagt ttcgcggtga attccacgat
      241 cggacgaatc aggtccgtat cgtagtttga cttcacgccc tgtaaaacaa cagcgagacg
      301 ctcgagcccc gcgcccgtat caaccgaagg cttcggcaat ggatcgagct tgtacttgcc
      361 cccctcagct tcgctgcgat caaactgcat gaagaccagg ttccagatct ccatgatcgt
      421 gtcgcccgga ccattcacgt tcgcggccga gttttccgga tcgtccgggc tctcacccat
      481 gtagtagtgg acctcgctgc atggtccaca cgggccggtc tcacccattt gccagaaatt
      541 atctttgcga ccaaagcgca ggatccgctc gcgcggcgca ccaacctgct cccagaaccg
      601 ctcagcgtct tcatcaggcg gcacttccgc gtcgccttca taaactgtga accagagtcg
      661 cttcggatcg agcttcagct catcaaccag cagttcccag gcaaacttga tcgcgtcttc
      721 cttgaagtaa tcgccaaacg aaaaattgcc gagcatctcg aagaacgtgt gatgccgcgc
      781 agtctttcca acttcgtcga ggtcgttgtg cttgccgccg gcgcgaatac acttctgcga
      841 cgacgcggcg cgcacatact cgcgcttctc ggcacccaga aagacatcct taaactgatt
      901 catgcctgcg ttcgtgaaga gcaacgttgg atcgttggcg ggcaggagcg gcgacgagcg
      961 cacccgcgtg tgcccgtgct cctcaaaata cctcaaaaaa gcttcgcgaa tttcgttacc
     1021 agtcatagtt gaatcgagtg taacaaatca gtcttcatcc gtgtaatcgt tggccaatga
     1081 acgcactctc tccctcacca actcgaattc aaacccctgc cgcacgaggt gttcgaacag
     1141 actcttcgct tccaaccggt tcttcggctt gccgcggatg cgcagacgtt tcgcaatcgc
     1201 ctcgtcgatc agttgttctt cgggagtctc gcaaaagacc atatcgaggg cttcatcggc
     1261 aacactgctg tcaacttttt tgaacttgag atcctgctcg aggcgcctgc gtccgaccgg
     1321 cttctgcttc actttgtagg atgcgtaact gaaagcgaaa cgctcgtcgt tcaggtaacc
     1381 atactcacgc agacgggcga taacggcttc taccacttct tcgttcgtgt ctttgcggtt
     1441 gagcaaacgc tcgcgcagct cgccgacaga tcgaggccgt gcggtcagca attttatggc
     1501 gcgctcaaat gttttacgtc gcaaatcagt cacgataatt gaacacagat taacgcggat
     1561 tggtcgctga tctaaaatgt ctgacatgga tggcgtatta gtaatcgaca aaccttcggg
     1621 catgacttcg cacgacatag tcgcgcaaac gcgtcgcatt ctccacgagc gtcgcatcgg
     1681 ccacaccgga acgttagacc cgtttgccac cggtgtgctc gtcgtcttac tcggcaaggc
     1741 cacacgtctt tcgcagttca tgactggagt tgataaacaa tacgaagcga tcattcgtct
     1801 cggatactca accgacaccg gcgaccgcac cggcaatccg attcctggac catcaaccga
     1861 agcacataac tggactgaac cagaaatcga agcagcgtta caaagccttc gcggaaacat
     1921 agaccaggta ccaccgatgt attcagcgaa gaaagttgag ggcaagaaac tctacgaact
     1981 tgcgcgtcgc ggtgagacgg tggaacgaaa acctgttcgc gtttgtatcc acgagttcgc
     2041 agcgatcaga cctgaaggtc aactcatcaa ggacaacctc gacggcacct tcgactttca
     2101 cgcgcgggtg tcgtgttcgt caggcactta tgtcagaacg ctggcggaag atttcggtaa
     2161 gcgtttgtac gttggcgctc atttagcgga gctgagacgg acgcgcgtcg gcgacctgcg
     2221 tgtcgatcag gcgatcacgc tcgatgagtt gaagatccat tttggcgaag actcgatcgg
     2281 cgaagtcctg ctgccgccgg gcacggcgct ctcatggctg ccttccctgc atctaagcgg
     2341 cgaagacgtg cggcgggcca atcacggaag agagttgaag gcgcttgaac gggattgggc
     2401 cgacggagag aacgtgagga tatacgacgt tggtgaggaa ttgattgcca tcggtcgcta
     2461 tgatgcgagt gcgcgaagtg tgcatcctca ggtggtatta agcggggaaa attgagtttg
     2521 caaaataatc attgacccct gtgctatact tcactcccaa ggaagcgcgc gtgcagttcg
     2581 cttccaatgt tcccaccgga cttcccgttt gaaatcgatc attgagaaga cgtgatgcct
     2641 cgaggacgta gctcacgctt acgcgcgcgt gaggcgtatc acgaagcaca gatcaaacca
     2701 gccgcagttc cccgacgagt gttgcggtgt aggcaaatgg cgtggctcct cacacgctgt
     2761 ccaatgcgcg aaggccgaag aggtttcaaa ggctatgcaa gagcaggatg gcaaaaaaca
     2821 attcaacatc agcgaacgtt tggaagatct gcgccgtgag cacgaagcgc tccgctggga
     2881 agggactttt cgcgactact tcgagttagt cgcccagaat ccgcgcgttg cccagctttc
     2941 acacgctcgc attaacgaca tggttcatgc cgccggcatc gagcggcata acgaaggcac
     3001 tcagcacgaa gttctctcct acaacttctt tgcccatgaa ctcttcggca tcgaagaacc
     3061 catcgccagg attgtcgagt atttcaaatc ggcggcgcag cgactcgaag tgcgcaagcg
     3121 catcctgctg ctcatgggac cggtaggcgg aggcaagtcg acgatcgtca acatgctcaa
     3181 gcgcggtctc gaagattgga ccaagactga cgccggcgca gtttacgcga tcaaagactg
     3241 tccgatgcat gaggagccgc tgcatctcat cccgcatcaa ctgcgtgctg aagtcgagaa
     3301 acattacggc atctatatcg aaggtgatct gtgtccgcag tgtcgctatg cgttggagca
     3361 cacgtatggc gggcgtcatg aagacgtgaa agtgcatcgt gtcgggttct cggaaaagga
     3421 gcgcgtcggc atcggcactt tctcgccgag cgatccgaag tcgcaggaca tcaccgagct
     3481 caccggatcg atcgatctct cgacgatcgg cgatgttggt gtcgagtcgg atccacgtgc
     3541 ttatcggttc gatggtgaat tgaatatcgc caaccgcggc ttgatggaat tcgtcgagat
     3601 gctgaaggtt gacgagaagt ttctctacag cctgctgacg ctctcgcagg agcagagcat
     3661 caagaccggg cgcttcgcga tgatctacgc cgacgaagtg atcctttcac atacgaacga
     3721 gaatgagtac attgcgttcg ccggcaatcg aaaatccgaa gcgttgcagg atcggatcat
     3781 cctcgtgagg gtctcataca acctccgcgt gtcggaggag gagcggattt acgacaagct
     3841 cttgcaccag tcggaagcgc tgcgcaacgt tcacctcgcg ccgcacacgt tgagagtcgc
     3901 agcgatgttt gcggtgatga cgcgcctcga agaaccgaag cgccagaacg tcgacatcgt
     3961 gaagaagatg aagctttatg acggcgagga cgttgaaggc tacaagtcga aagacgtgcg
     4021 cgagctgaag gaagagacga tccgcgaagg catggatgga atctcgccgc gttacatcat
     4081 caaccggctt tcaagcgcgc tggtgcgcga tggggtcacg tgtatcaatc cgatcgatgc
     4141 gctgcgagcg atcaaggacg ggttcgaaca acacaccggg attagcagcg agcagcgtga
     4201 aaggtatttg aatcatattt cgcaggcgcg caaggaatac gacgagctcg cgaagatcga
     4261 agtgcagcgc gcgtttgttt actcgtttga agagatggcg cgcacgatgt gcaacaacta
     4321 tctcgataac gttgaagcgt tctgcaacaa ggagcgcatt aaggatccga tcactgaaga
     4381 agagatggat ccggacgagc agttgatgcg gtcgatcgaa gagcagatcg ggatctcgga
     4441 aaacgcgaag aatactttcc ggcaggagat cctgattcgt atctcgtcgc acgctcgcaa
     4501 ggggaaggcg tttgagtaca attcgcacga gcgtctgaag gaagcgatcg agaagaagat
     4561 cttcgctgat ttgaaagatg tggtgaagat cacgacgtca acgaagacgc ccgatccgga
     4621 tcaactgcga aggatcaacg aggtcatcga tcggctcgtg aaggagcacg ggtattgtcc
     4681 ggtttgtgcg aatgagttgt tgacctatgt aggtacgttg ttatctcggt agatatttag
     4741 ccacagatta cacagataaa tacggatttt taatccgtgt catccgtgta atctgtggct
     4801 aaaaatatga ggatgaagaa acgttactgg attgcaggtg cggcgacact cgctattgca
     4861 ggcaaacttt tgacgcggcc gcgtgatgcc gattggcaga agtgccgcga cgtcattttt
     4921 cattccgagc attcgtgttt cattgacgtc gatggcattc gcgttcatta ccaggaagct
     4981 ggcgatccaa aagcacccgc gctcgtcttg attcacgggt tcgcgtcgtc gacgctcgtg
     5041 tggagcaaag tgttcttgcg attagctgaa gcgggttatc gagtgatcgc gctcgactta
     5101 cttggttacg gttattccgc gaagccaaag aatggcgagt acacgattgc aggacaggcg
     5161 aaactgttaa cgcgcttgct cgatgcgctt ggaatcccgc gcgcgattgt tgtcgggagc
     5221 tcgtacggtg gtgcagtcgc tgcgacatgt gccctggatt atccggatcg agtcgagaag
     5281 ttaatactgg ttggcgccgt gaacaataac cagccgtgcg actttatgtt gatgcgcttc
//
LOCUS       DASSFQ010000199         6440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1420569, whole genome shotgun sequence.
ACCESSION   DASSFQ010000199 DASSFQ010000000
VERSION     DASSFQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420569"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3387
                     /locus_tag="VFH96_06930"
     CDS             <1..3387
                     /locus_tag="VFH96_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET6973743.1"
                     /translation="ISKGHIEISRLTLGVGRSRLAAALAQLYREARGKGLSLEELAIA
                     TARVHATEEDHARALDDLEATMDEFLGVRRTTPAARLNRSEVLEAWTETQKFLQTIPS
                     HETLADFCRLVENFRSTRPQARAPVADHVKALDALVWGKDLQGRVPQVCLDLFARQYG
                     LEMINLLLRVDQRLNEEKQKLAALDFDDLELRTLALLERPEVIARTSERYRFFLVDEF
                     QDTNAVQRVLLERLALAKGRRPANLFIVGDRKQSIYGFRGADVDVFREMTSILLEAGG
                     EEKPLQLNFRSQPPLIAFFNHLFARLFRVPDDVPASEYKNLDQLGYVKHEMSEAKREL
                     RDSGPLVEMLITTKPSLADDPRAEQDSRVLDAQQLARRILALTKGPNAVKYGDIALLF
                     RAMTQVSIYESVFRRANIPYQTVLGRGFYEREEITDLIQLLRFLDNKTDELALAAVLR
                     SPLCGVSDNALLALRCAPLLSEVETGDPVRGFSATRPLYAALRRHEEIAFISAEEHEL
                     LDRAAKLIGELVARRHHYPIHDLLRFAVQESEYLTVVAANFDGAQRLANVERLFTLAA
                     RFESSGTHLIRDFVRYVQEFEAIGSRESEGQIDQAADAVRLMTIHQAKGLEFPIVIIP
                     DLQRLSRITSDNWFLLDRHQGLTLKVPDGRGNLVAGCTFANFEQRHAWREQFESMRLL
                     YVAASRAEDRLILSGVTEDIANMSTKNDSWLKWIWQALELGECRASDLIDLADEVQLQ
                     LTMNLGDEAEEFELEPESPPQTSMYSADSLNEAFPLVKPLGPQSSHAIHRFSVTQLIN
                     YQRCPRQYYFDRVLQLAAADQMAVWNNAEAPEPPANLTATLKGSVIHRFCERYTSDQS
                     AEECLRTSFDEVLRLRQAQLADRFVEIDHEAAIKEMLPLANNYLASDVFKRIGAAPKI
                     EEHDSARPAGEDGLWSELSFRLRRQLGVLNGIIDKLLITRSANGTAEIEIIDFKTNRI
                     YYDKSETPPEPLPRRIRPPKEDQFAFDFEAAPPPKPVNRHPSLAEALKTAAEDYQLQM
                     QAYALAVRELLPSLSNAKIRVTLHFLDPNVEVNLADELLERTRCEQAIDEGMQRTIFS
                     SDPVNFPVKTAPHCRMCNFLSVCTAGKDWMRENWATDDPD"
     gene            3416..4627
                     /locus_tag="VFH96_06935"
     CDS             3416..4627
                     /locus_tag="VFH96_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6973744.1"
                     /translation="MITIKAFDGGEFDAYLALPASGYGPGIVVLQEIFGVNSFMRGVA
                     DWYAAHGFVALCPDLFWRLERGVELTEADRPKAFQLYQDLDEDLAVQDSAAAVKFLRK
                     HPACNGRVGAVGFCLGGNLAYLLSVRYKPECAVGYYGVGIEKSLDEATSLDNPLLLHI
                     AGKDQFCPPGAQAQIHATFDANPLVTIHDYPEQGHAFGRVGGDHYDAAAAELAHLRSL
                     EFFITNLAGISVTSTQQILSDRWDEHVKYEFATRNTDDTLQTMVVDSYVNHVPVMTGG
                     VGHDELREFYSQRFIPQMPPDTAMTPVSRTIGIDRVVDEMVFEFTHTIKMDWMLPGVE
                     PTGKHVKVALVVIVHFRDGKLAHEHIYWDQASVLAQLGLIDAQTLPVAGVESAEKVLN
                     PRLASNELMNR"
     gene            4641..6008
                     /locus_tag="VFH96_06940"
     CDS             4641..6008
                     /locus_tag="VFH96_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6973745.1"
                     /translation="MKPTDRLRRDLGTLESYAAVIGILIGAGIFQVTSQAWALTGPSV
                     ILGYIVLTPAILATSIPYAAYLSTPLGREPGGEYLHISRTLNGHRLAYVGAWLKIISY
                     IGAGAYLANAMAGYIIGNNVEAQRYRLPLALLALVVFYLIHVVGIRWFGRFQVTFSAL
                     KCLALLVLIVPGLFVLNKTNYTPFFTHGPSGFGASLLPLFFAYAGFESIAQTAGEVKD
                     STRRLPMILLKGITITALIYVFTSVVSFGVLPGGRLQASNAPMAEVASIYLPPGAAIF
                     VTIGAIAAIMTALNGTIFVPSRLAIMFVEDRLAPGWLGFINKKTGTPISGLTLTLICC
                     AALLISRQIALALNVAVFALVVLYFIHSLVFLFLPRLNPPLASEISISLPRRLQQAAA
                     VVSVLSMGLMILIQLRQDVEVLRTQTLAQRIMGQSLTSIELIVVWGVIGAILYQLARM
                     QRSTS"
     gene            6069..>6440
                     /locus_tag="VFH96_06945"
     CDS             6069..>6440
                     /locus_tag="VFH96_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973746.1"
                     /translation="MIEHRHREEFPTLASGIHLLSHSLGPVPRAARDSMLAFIDAWEH
                     HTSEDAWATSWWELSQRVGDRIARILGGAPGSVQIEPNASLALATAASCFDFKSGARR
                     KVVTTDLDFPSTEYFWDGQKQI"
BASE COUNT         1351 a         1789 c         1832 g         1468 t
ORIGIN      
        1 atcagcaaag gccatatcga gatctcgcgc ttgacgttgg gcgtcggccg gagtcgtttg
       61 gcggcggcgc tggcccaact ctaccgcgag gctcgcggca aggggttgag tctcgaagaa
      121 ctcgcgattg caacggcacg cgtacacgcg accgaagaag atcatgcgcg agcgctcgac
      181 gaccttgaag caacgatgga tgagtttctc ggcgtgcgtc gcacgacgcc agcggcgcgg
      241 ctaaaccgtt cggaagttct cgaagcctgg accgaaacgc agaagttttt gcagacgatt
      301 ccttcgcacg aaacgctcgc cgacttctgc cgtctcgttg agaatttccg atccacgcgc
      361 ccgcaagcgc gcgcgccggt tgcggatcac gtgaaagcgc tcgacgcgct tgtttgggga
      421 aaagatcttc aaggccgcgt accgcaagtt tgtctcgatc tgtttgcgcg gcaatacgga
      481 cttgaaatga tcaacctgct gttgcgcgtc gatcagcgac tcaacgaaga gaaacaaaaa
      541 ctcgccgcgc tcgactttga cgatctcgaa ttgcgaacgc ttgcattgct ggaacgtccg
      601 gaagtgatcg cgcgcacgtc cgagcgttat cgcttctttc tcgtcgacga atttcaggac
      661 acgaacgcgg tgcagcgtgt gttgctcgaa cgtctggcgc tcgcaaaagg ccggcggccc
      721 gcaaatctct tcatcgtcgg cgatcgcaag cagtcgattt acggttttcg tggcgcagac
      781 gtcgatgtgt ttcgtgagat gacgtcgatc ctgctcgaag ccggtggcga agagaagcct
      841 ttgcagttga acttccgtag ccagccgccg ctgatcgcgt tcttcaacca cctctttgcg
      901 cgactgttcc gggtgcctga cgacgtgccg gcgagtgaat ataaaaacct ggaccagctt
      961 ggttacgtga agcatgaaat gagcgaagca aagcgagagc tccgcgactc aggcccgctg
     1021 gttgagatgc tcatcacgac gaagccgtcg ctcgcagacg atccacgcgc cgaacaagac
     1081 tcgcgcgtgc tcgacgcgca acagcttgcg cgaagaatac tcgctctcac aaaaggacca
     1141 aacgcagtta agtacggcga cattgcgttg ctctttcgcg cgatgacgca ggtatcgatc
     1201 tacgagtcgg tttttcgacg cgccaacatc ccgtatcaaa ccgtgctcgg acgcggcttc
     1261 tacgaacgtg aagagatcac cgatctaatc caattgctcc gctttctgga taacaaaaca
     1321 gacgaactcg cgctcgcggc ggtacttcgt tcgccgttat gcggagtctc cgacaacgcg
     1381 ttgttggcct tgcgatgcgc gccgctgctg agcgaagtcg agaccggcga tccggtgcgt
     1441 ggtttctcag cgacccgccc actatacgcc gcactgcgtc gtcatgaaga gatcgcattt
     1501 atcagtgctg aagaacacga gctgctcgat cgcgcggcga agttgatcgg cgaactcgtg
     1561 gcgcggcgtc atcactatcc gatccatgat ctgctgcggt ttgccgttca ggagtcagaa
     1621 tatctaacag ttgtcgcggc gaattttgac ggcgcgcaac gtctcgctaa cgtcgaacga
     1681 ttgttcactc ttgccgctcg gtttgagagt tcgggaacac acttgattcg cgactttgtg
     1741 cgttacgtgc aggagtttga ggcgattggc agtcgcgaga gtgaaggaca gatcgatcag
     1801 gcagcggacg ccgtgcgctt gatgacgatt caccaggcga aaggtctcga gtttccgatc
     1861 gtcatcattc ccgatctgca acgactctcg cgtatcacca gtgacaactg gttcttactc
     1921 gatcgtcatc agggtctgac tttgaaggtg cctgacggcc gtggaaatct cgtcgccggt
     1981 tgcaccttcg cgaatttcga acagcgacac gcctggcgcg aacagtttga aagcatgcgt
     2041 ttgctctacg tcgcggcgtc gcgtgctgaa gatcgattga ttctctccgg cgtgacagaa
     2101 gacattgcga acatgagcac gaagaacgac tcgtggctca aatggatctg gcaggcactc
     2161 gaactcggcg agtgccgcgc cagtgacttg atcgatctgg cagacgaagt gcagttgcaa
     2221 ctgacgatga atctcggcga cgaagccgaa gagtttgaac tcgaacccga atcgccgccg
     2281 caaacgagca tgtattcagc cgactcattg aacgaggcgt ttccgctcgt gaaaccgctc
     2341 gggccgcaaa gtagccacgc gattcaccgt ttcagtgtca cgcagttgat caattatcag
     2401 cgctgtccgc gtcaatatta tttcgaccgc gtcctccaac tagcggcggc cgatcaaatg
     2461 gccgtttgga acaacgccga agcgcccgag ccgcctgcga atctgaccgc gacgttgaaa
     2521 ggctccgtca ttcatcgttt ctgcgagcgt tacacttcgg atcagagtgc cgaagagtgt
     2581 ttgcgcacga gctttgacga agtgctgcgc ttgcggcagg cgcaactcgc cgatcgtttt
     2641 gtggaaatcg atcacgaagc ggccatcaaa gagatgttgc cactcgcgaa taattacctc
     2701 gcgagcgacg tgttcaagcg catcggggct gccccgaaga tcgaagagca tgactcggcg
     2761 cgtccggccg gtgaagatgg actatggagt gagctcagct ttcgtttacg gcggcagctt
     2821 ggtgtcttga acggcatcat cgacaaactc ctgatcacgc ggtcagcaaa cggcactgcc
     2881 gaaatcgaga tcatcgactt caagacaaac cggatctatt acgacaaatc agaaactcca
     2941 cctgagccac tgccacgccg tattcggccg ccaaaagaag atcaattcgc gttcgacttc
     3001 gaagccgcgc ccccgccgaa gccggtcaat cgtcatccat cgctcgcgga agcgttgaaa
     3061 actgcggctg aagattatca actgcaaatg caggcgtacg cgctcgccgt tcgcgagcta
     3121 ttgccgtcgc tttcgaacgc aaagattcgc gtgaccttgc actttctcga tccaaacgtc
     3181 gaagtgaatc tcgcggatga gttgctcgaa cgcactcgtt gcgaacaggc gatcgacgag
     3241 ggaatgcagc gcacgatctt ctcttcggat cccgtaaact ttcccgtcaa gaccgcgccc
     3301 cactgccgta tgtgcaactt ccttagcgtg tgcaccgcgg gtaaagactg gatgcgagag
     3361 aattgggcca cggatgaccc ggattgatcc gtcttttccg tgttaatctg tggccatgat
     3421 cacaatcaaa gccttcgacg gtggtgagtt cgatgcttac cttgccttac ccgcgagtgg
     3481 ttatggacca ggtatcgtcg tgcttcagga gatctttggc gtcaatagct tcatgcgcgg
     3541 tgtggctgac tggtacgcgg cgcacggatt tgtcgcgttg tgtccggatc ttttctggcg
     3601 tctcgaacgt ggtgttgagc tgacggaagc cgatcgaccg aaagcctttc agctatacca
     3661 ggacctcgac gaggacttgg cggtgcagga ctcagcggct gcggtcaaat ttctgcgcaa
     3721 gcatccggcg tgcaatggac gcgtgggcgc ggtgggtttt tgtctcggtg gcaatctcgc
     3781 ctacctgttg tcggtgcgtt acaagcctga gtgtgcagtc ggctattacg gcgtcggtat
     3841 cgaaaaatcg ctcgacgaag caacgagcct ggataatccg ttgttgctgc acatcgcggg
     3901 caaggatcag ttttgtccgc ccggggcgca ggcacagatt cacgccacat tcgatgcaaa
     3961 tccgctcgtg acgattcacg attaccccga acaggggcat gcattcggtc gtgttggcgg
     4021 tgaccattac gacgcggcag cggcggagct tgcacatctt cgcagtctcg aattctttat
     4081 aactaatctc gccggcatca gtgtcacttc aacgcaacaa attctgtcgg atcgttggga
     4141 cgaacacgtt aagtatgagt ttgcgactcg gaacactgac gacacgctcc aaacgatggt
     4201 ggtggattct tacgtgaatc acgtgccggt gatgaccggc ggcgtcggcc atgacgagct
     4261 gcgcgagttt tattcgcagc gtttcatccc ccaaatgcca cccgatacgg cgatgacacc
     4321 cgtgtcacgc acgattggca tcgatcgcgt ggttgacgag atggtttttg agtttacaca
     4381 caccatcaag atggattgga tgttgccggg agtcgaaccg accggcaaac acgtcaaagt
     4441 agcgctcgtc gtgatcgttc actttcgtga cggcaaactc gcgcacgaac acatctactg
     4501 ggatcaggcg tcagtgctgg ctcaactcgg gttgattgat gcacagacgc ttcctgttgc
     4561 cggtgtcgag agcgctgaga aggtgctcaa tccaagattg gcgtccaatg agttgatgaa
     4621 tcgttgacag gattaaccag atgaaaccta ccgaccgcct gcgacgcgat ctgggcacac
     4681 tggaatcgta cgctgccgtc attggcatac tcatcggcgc gggtatcttt caggttacca
     4741 gccaggcgtg ggcgctgact ggtccaagcg tgattctcgg ttacatcgtc ctgacacccg
     4801 cgattctcgc tacctcgatt ccttacgccg cgtacctttc aactccactc ggacgagaac
     4861 cgggcggcga gtatctgcac atctcgcgaa ctttgaacgg ccatcgtctc gcatacgtcg
     4921 gcgcgtggct caagatcatt tcttacatcg gcgcgggcgc gtatctggcg aacgcgatgg
     4981 cgggttacat catcggcaat aacgtcgaag cgcaacgtta ccgtttgccg ctggcactgc
     5041 tcgcactcgt tgtcttttat ttaatccacg tcgtcggcat tcgctggttt ggtcgctttc
     5101 aggtgacgtt ctcggcgctg aagtgtttag cgttgctcgt gctgatcgtg cccggtttgt
     5161 ttgtgctcaa caaaacaaac tacacgccat tctttacgca tgggccgagt gggtttggcg
     5221 cgagcttgct gccgctattc tttgcgtacg ccggcttcga atcgatcgcg caaacagctg
     5281 gtgaagtgaa agacagcacc cgccgtttgc cgatgattct gttgaaggga atcacgatca
     5341 cggcgctgat ttacgtgttt acctcggtcg tgtcgtttgg cgtattgccg ggcggacgct
     5401 tgcaggcgag caacgcgccg atggctgaag tcgcatcgat ctatttacca cccggcgcgg
     5461 cgatatttgt cactatcggc gcgatcgcag cgattatgac tgcgctcaac ggcacgatct
     5521 tcgttccatc gcgtctggcg atcatgtttg tggaagacag acttgcgcct ggctggctcg
     5581 gattcatcaa caagaagaca ggtacgccga taagcggctt aactctaacg ctcatttgtt
     5641 gcgcggcgtt actgattagt cgtcaaattg cgctcgcctt gaatgttgca gtttttgccc
     5701 tcgtcgtgct ttatttcatt cacagcttag tattcctgtt tctcccccga ctgaatccgc
     5761 cgcttgcgag cgagatttcg atcagtctgc cgcgtcggtt gcagcaggcc gcagccgttg
     5821 tttcagtgct gtcgatgggg ttgatgatac tgattcagtt gcgtcaggat gtggaagttt
     5881 tgcggacgca gacgttggcg cagcggatta tggggcagtc gttgacaagc atcgagttga
     5941 tcgtcgtttg gggcgtgatc ggagcgatac tgtatcaact cgcgcgcatg cagcggagta
     6001 ctagctaagg acgcccttgg tttagagtaa gttttacagg aaaatcttct taatcctgtc
     6061 aacagctgat gatcgaacac aggcaccgcg aagaattccc aactctggct tcgggcattc
     6121 atctgctgag ccatagcctc ggcccggtgc cgcgtgcggc gcgcgactcg atgctcgcgt
     6181 ttatcgacgc ctgggaacat cacacgagtg aagatgcctg ggccacaagc tggtgggaac
     6241 tctcgcaacg tgtcggcgat cgcatcgcgc gcattcttgg cggcgcgccc ggttccgttc
     6301 aaatcgaacc aaacgcttcg ctcgcactcg ccaccgccgc gtcgtgcttc gatttcaaga
     6361 gtggcgctcg ccggaaagtc gtcacgacgg acctcgactt tccttcaacg gaatatttct
     6421 gggacggaca aaaacagatc
//
LOCUS       DASSFQ010000200        34715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1124473, whole genome shotgun sequence.
ACCESSION   DASSFQ010000200 DASSFQ010000000
VERSION     DASSFQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 34715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34715
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124473"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFH96_06950"
     CDS             <1..840
                     /locus_tag="VFH96_06950"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET6973747.1"
                     /translation="KGVDAKKTLDSIRLLKDAGVDGVNIPDGARAQSRMSALATAVLV
                     EQQIGIETVLHYCCRDRNLLGMMSDLLGAAALGLRNILVITGDPPKMGPYPDATAVFD
                     IDSIGLTNMVNKLNHGLDLGNNPIGEPTSFCIGVGVNPGAINLDEEIRRFEWKVEAGA
                     QYAITQPVFDTNQLRDFLKRIEHVRIPIIAGIWPLVSYRNAEFLHNEVPGVRVTPSIM
                     DRMRAASNISKEAGRDEGLKIARESLLEVRDEIQGVQVSAPFGNVKYALEVFEALPEF
                     SRG"
     gene            complement(861..1757)
                     /locus_tag="VFH96_06955"
     CDS             complement(861..1757)
                     /locus_tag="VFH96_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HET6973748.1"
                     /translation="MRILVSGSHGLVGQALISSLTSDGHEIVRLVRSKPAGATEIEWH
                     PNQEKIDAASLEGLGAVVHLAGESIASSRWTEEKKRAIRDSRVKGTALLSEALARMSR
                     PPSVFVSASAIGYYGNRGDEVLTEQSAPGDDFLANVCVEWENATIPAIEKGIRTVHAR
                     FGIILDQKEGALAKMLTPCRMGIGGRIGDGKQWMSWIDIQDVVNGLKFLIGNSSVNGP
                     VNFVAPNPVTNAEFTKTLGRVLSKPTIFPIPAFGARLAFGEMGDALLLSSARVKPGVL
                     QNFAYPTLDSALRHLLNADKRG"
     gene            1919..4240
                     /locus_tag="VFH96_06960"
     CDS             1919..4240
                     /locus_tag="VFH96_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET6973749.1"
                     /translation="MLRLTTFFLISVFTFIVIAICGPGRVRSVSTLTRLTNTPEHVLN
                     LNPAISDDGRTIVFESSADLANTGGSASFRLLRADPSFVELGRTRAVCPAMSGDGRTI
                     VFASREDLVGRNADRNSEIFLFDGVKLNQLTETSGASNLQPSISSDGRAVAFVSNGEI
                     VLYDTLDRKSTRLGSGVSPKISGDARYVYYTRNLNADNADLIRIDVETLTSRVLLANV
                     AELSIVEGRAVSNDGMRVVYSAATGLNQTQVFMFDGRDESIRQLTNLGSRAVDVGLQP
                     TISGDGRRVAFATRRRVVGLSDGGVELYVLDLPTGVIQQITNAPSTATGEVVSSLNFD
                     GSAVAFNFPRILSGAVNDDDLRNNSEIYVASIAPRPIGTATVVNAAAPELANIALGSI
                     ASIRGSALGPSVAVNGQAAQIFYSSPQEVVFLVPSGLIVGPAQFLVTNAEGLQSKAEV
                     IISPTAPGVFNNAGEAIVLNSDTLTPGPFDPSKGELRLSIFVTGVARAGNVLVTINSK
                     PVIVETVVPASLPGLDEIHVLVPAELRGAGKSTLVVSADDVQSNSVSLVLTGATPTPT
                     PTPTPTPSPSPSPSPTPTPSPGSSPHIVISQVFGGGGNSGAPFRNDFIEIFNAGQTPV
                     NLSGWSVQYASATASTWAVTPLTSVTLNPGQYYLIQESSGGSNGVLLTAPDATGTIAM
                     AAGSGKVVVTKTSTTLTGTCPNDPNIVDLVAYGNTANCFKGSAPAPTPSNTNAIQRAA
                     AGCTDTRNNVADFALGPPNPRNSNFLPRICTDP"
     gene            4265..6118
                     /locus_tag="VFH96_06965"
     CDS             4265..6118
                     /locus_tag="VFH96_06965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET6973750.1"
                     /translation="MHSLTDAVASLRQHFGFEDFREGQREVIGSILEGKDAVVVMPTG
                     SGKSLCYQLPAMILDGVTLVVSPLIALMKDQVDALLARGLPATFINSSISESEQRARI
                     DALKRREHKLVYIAPERFRSSRFNAALQQIPISLFAVDEAHCISTWGHDFRPDYLRLR
                     NVIKSLDKVQTLALTATATPYVRSDIIQQLGLDRPQTFVSGFDRPNLSIEVDHTERER
                     QKIARIRNLAHQNPGSGIIYASTRKAVEQVGAKLRALDLKVSEYHAGMSDALRIRAQE
                     DFMSGRTQMIVATNAFGMGIDKPDIRFVVHYQMPGSIEAYYQEIGRAGRDGLPSTCVL
                     LFNYADKNTHDFFIEGSYPNISIIKQVYDSLAGTELKRIELSTQEIARRAGETNEMAV
                     QSALYMLERAGHIQRTAPALNRGGRGAQQGRSIIMLDNEPVAKLRIDPAEASRRGELE
                     RRKLRAMIDFCYTDRCYRAYILNYFGDRKSERLCGTCGNCEPTSTTSIPSAPVDFAPR
                     TPTDDELLRVRKILACAKRMNGRFGKKMLISTLRGSSAKQLLTAKLNELSTYGLLRDM
                     ANDELEAYIDALIEAHCLRVKPGAYPTISTTELGDRVMREQEQIALSLKSV"
     gene            complement(6136..6933)
                     /locus_tag="VFH96_06970"
     CDS             complement(6136..6933)
                     /locus_tag="VFH96_06970"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET6973751.1"
                     /translation="MSTAATAASPKEKLIIAGREFSSRLIIGTGKYRSYEQMRSAHEA
                     SGAEMVTVAVRRVPLDRSSESFLDYLSPALQILPNTAGCYTAEDAVRTARLAREALQT
                     DLVKLEVIGDQTTLFPDNEQTLEAARILVKEGFVVLPYFNDDLIMAKKLLDAGCAAIM
                     PLAAPIGSGLGVQNPANLRIMREQLPDATMIVDAGVGTASDAAIAMELGADGVLMNTA
                     IAEAEDPSKMATAMKLAIEAGRLAYVAGRMTKRLYASASSPLQGVIK"
     gene            complement(6934..7131)
                     /gene="thiS"
                     /locus_tag="VFH96_06975"
     CDS             complement(6934..7131)
                     /gene="thiS"
                     /locus_tag="VFH96_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET6973752.1"
                     /translation="MKVFVNDELREVEVSTLAELIAELDLPVARIAIELNRDVVRRSD
                     WGSTMLKDEDRIEIVHFVGGG"
     gene            complement(7205..8065)
                     /locus_tag="VFH96_06980"
     CDS             complement(7205..8065)
                     /locus_tag="VFH96_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973753.1"
                     /translation="MITGFNTDIEHDGVTYHVQTEDKGLDSPIILSLVYVGGTILASK
                     RSPYGDLIASGFSDEVLSERLKRQHRLICAAINSGRIDDLKKMSGRPKEAPQKTVEEA
                     APEVEAPSVSTVDEPFEIEYWPVSQEWTPPPTPEDEELAAVMEPGMEPRYEPVDLTAK
                     APEPEPEVEEPLEVEEFVSEPDLEDGLFIELLDDDEFYSGQRYTLRVLVNNRTNGDEK
                     PLANVAVSVKVLGTTFRPLIYTLKTESDGVASISTDIPQFNSGRAAVLIRAVTKDQAA
                     ELRRLIHPKD"
     gene            8360..8836
                     /gene="ndhC"
                     /locus_tag="VFH96_06985"
     CDS             8360..8836
                     /gene="ndhC"
                     /locus_tag="VFH96_06985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET6973754.1"
                     /translation="MSFRLYDYLPIVLMFIVAGGFAVGNVLLSQFVGQRKRTRTKLMP
                     YECGKDPVGSARERFSVKFYLIAMIFILFDIEVIFLVPWAAVYKALAAQGPAMRSFAY
                     LEMMIFVGLLFVGYIYIVKKGAFDWGERARREAEAEARALADLEARKVEKRRRPAA"
     gene            8847..9476
                     /locus_tag="VFH96_06990"
     CDS             8847..9476
                     /locus_tag="VFH96_06990"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET6973755.1"
                     /translation="MGLENVIAEALPEVLTTRLDSLINWARKSSLWPATFGLACCAIE
                     MMNATSSRNDLARFGSEVFRASPRQADVMIVSGRVSRKMAPVLRRIYDQMPEPKWVIS
                     MGACATSGGVFDNYAIVQGVDKIVPVDVYIPGCPPRPEMLIHAVMMLQDKVMKESVRD
                     RRDIPEEEAREAVVPGTLPITDGSTAQQNTLPESRPYTIASRHERRRST"
     gene            9498..10007
                     /locus_tag="VFH96_06995"
     CDS             9498..10007
                     /locus_tag="VFH96_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET6973756.1"
                     /translation="MTTEQTPSSEIAPSPSVTALQREHPEWVREVIVAFGETTIVVPR
                     ENIVDACLFLKNSPETRFDFLADICGADRGVEEEPRFEVNYHLFSTTKYHRVRLKVLL
                     NEEDTHVPSVTGVWRTSNWHERETFDLFGVIFDGHPDLRRILLPDDWQGYALRKDFPL
                     RGYEPYSLK"
     gene            10049..11263
                     /gene="nuoD"
                     /locus_tag="VFH96_07000"
     CDS             10049..11263
                     /gene="nuoD"
                     /locus_tag="VFH96_07000"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HET6973757.1"
                     /translation="MSSVTIPPQFEIVDRPLDTQMTISMGPQHPSTHGVLRLELVLDG
                     EMVVKATPDIGYLHTGMEKLFEYKKYQQGIVITDRMDYLNPLGNNLAYVMAGEKLLGL
                     EIPERAQTIRVLMCELQRIGSHLVWLGTHALDIGAMTVFFYCFREREKILNLIEAASG
                     GRLTPSYFRIGGLMMDLPAGFDNRCKQFLDDFPPALDEFHKLLSGNKIFQRRTQGIGR
                     ISAEDAIDWGLSGPCLRGSGINLDIRRANPYSGYEKYDFEVPTEPDGDVWARYMVRMR
                     EMKESHRIATQALSRLQPGPIKADAPKVVLPDREAMKSHMDALIHHFLIVAEGFDVPA
                     GEVYHPIEASKGELGVYMRSDGGPKPSRVHFRGPSFVNLSALPVMSEGEMVADIVAII
                     GSLDIVLGEIDR"
     gene            11338..11868
                     /gene="nuoE"
                     /locus_tag="VFH96_07005"
     CDS             11338..11868
                     /gene="nuoE"
                     /locus_tag="VFH96_07005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HET6973758.1"
                     /translation="MSTQIVNIQPLSQPVDLGVEPATFSPQVEAEIDRHLAKYPVMRS
                     AILPLMFIVQRERGYLDPPGVLYLANRLKVRVTDIWEVATFYSMIHTEPIGKYHIQVC
                     KTLSCKIMGAGKVTEHCSQKLGIKVGETTADGRFTLSEVECLGSCGTAPMFQIGFDYH
                     ENLTPEKIDQILDKLP"
     gene            11868..13157
                     /gene="nuoF"
                     /locus_tag="VFH96_07010"
     CDS             11868..13157
                     /gene="nuoF"
                     /locus_tag="VFH96_07010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET6973759.1"
                     /translation="MRIAAIGCEPLQLRRVHLEWSHTLDVYRRNGGYQALKKVLDGMS
                     PDDVINEVKKSALRGRGGAGFPTGMKWSFVPKDSPKPKYVVCNADESEPGTFKDRYLM
                     ERDPHMLIEGMLIAAYALNSKTCYIYTRGEYKYLIDIMDVALEEARKAGLVGDKILGT
                     DFSCEIHTHTGAGAYICGEETALLSSLEGLRGHPRMKPPFPAVSGLYAAPTVVNNVET
                     LTAVPDIIKMGGEAYQKLGTEKSGGTKLWSVSGHVKLPGVYELPMGYDDMEKFIMQDC
                     GGMLNDKQLKACIPGGSSVYIMRADQIIGKDVRMDYEGLVAAGSSVGSGGFIVMDETV
                     DVFESTKNLTEFYKHESCGWCTPCREGTDWLVKIFKRIENGGGRPDDAQLMLDLVDNI
                     EGKSFCPLGDAASWPIQSAIKRFPEDFRKRLSTDYTD"
     gene            13193..15460
                     /locus_tag="VFH96_07015"
     CDS             13193..15460
                     /locus_tag="VFH96_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6973760.1"
                     /translation="MSTEQVKVTINGKDFEVPKGARLIDVCREKGFDIPSFCYYSDLA
                     LQASCRMCLVRIEKMPKLQTSCTITCTDGMIVTTQSEEIEKAQRGMTEFLLANHPLDC
                     PVCDRGGECELQEMTFDWGGLEERFTEKKNYYAERYLSPMVANDPQRCILCKRCTRVC
                     DEWMGEDAIEAGGRGAHTVIGTYLGWLDCSQCGNCIEVCPTGTLLDATYRHQTRPWEL
                     AQTITTCNFCSDGCQMSLGSRGGEVRRAVARDRYVNGLNGEFLCVKGRFGHPFINHED
                     RIRTPMIRYKKGGKLIPATWDEAIRHVAERLDQVADAHGRNSIGVVGSPRLTNESLYV
                     LRKFATELVGTENYTTTDLFSLKNFFNNLGAQLATHRDIRYAKTIVQIGGDPSELQPL
                     TGKQMRQAVRNGGATLVLINSVPIRLREQAQVFLHVRPGTEDAIVLALAGSSNDSLAA
                     QKAGVEASAIDAARQAIANTEGDVVIMFGHEVSEAAQGVIAQLSQTFGGDKRRILLHP
                     LPFFNNSVGAHAVGMMERPTSPRQILDAAGGEIRALYMAGSFLPEHLKGRDAALSQLD
                     FLVVQELFENETTAAADVVLPAASYAEQDGTFTNNDGFVQRVRQSIPPLHQSKPDWMI
                     VAQLAKELGLDFGYEMSASAVFREIGEHVPAFSGMRYPLLKDEKNPIQAKFEVGSSDV
                     SDALTSIRDHVESLPDEGDKITMTPDVGTELFRLGGLTEKVGQFQLLASGNPRPETTA
                     VSPLYQIQSVLSVAK"
     gene            15465..16517
                     /gene="nuoH"
                     /locus_tag="VFH96_07020"
     CDS             15465..16517
                     /gene="nuoH"
                     /locus_tag="VFH96_07020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET6973761.1"
                     /translation="MDTAIDIVLKLVAITVAFTVVLLIVAYTVLAERRVLGLIQGRLG
                     PNRVGYGGVLQPFADLLKFILKEDIVPDKSTRFIYFLAPVIATMAALMTMIVYPFGPE
                     IAIPFTNITIPLTIARFDIGLLYVLGITSIGVYGIMLAGWSSNNKYSLMGGLRSSAQM
                     ISYELALGMSLIGVIMMAGTLDLGQIVENQSGWHGTHWYIVFQPLGFIIYLIAAIAET
                     NRVPFDLPEAETELVAGFHTEYSAMKFALFFLAEYINMFTVSMLATTLFFGGWNFPFL
                     DLALTRGTFLFGLVSALGFIFKICVFLFIYIWLRGTLPRFRFDQLMNFGWKFLLPMAL
                     LNIFLTATLIYFFPNL"
     gene            16737..17396
                     /locus_tag="VFH96_07025"
     CDS             16737..17396
                     /locus_tag="VFH96_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET6973762.1"
                     /translation="MLALLFILFAGLAIGCGLAVVAQRNPLYSAISLIGVFISLACLY
                     VMLAAPFIAAVQVIVYAGAIMVLVVFVIMLLNVEQEERGRPRLKFLVPVAVALSAILI
                     AEVAFILVSVQEFRVNPTSVPSDVGLTHSIGAQLFTRYLLPFEITSILLLMAIVGAMT
                     LARRGGLLPPNARIVPGAQVLPLSMVDPNAEPDDLRPVEADSSDEAARVLGITDFQKR
                     D"
     gene            17401..17706
                     /gene="nuoK"
                     /locus_tag="VFH96_07030"
     CDS             17401..17706
                     /gene="nuoK"
                     /locus_tag="VFH96_07030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET6973763.1"
                     /translation="MEPQLSWYLALSAILFTIGVTGVIIKRNVIAMFMCIELMLNAVN
                     LTFVAFASFFQDVTGQLFVFIVMTVAAAEAAVGLGIIIAIFRNRESLDVDEASILKL"
     gene            17751..19853
                     /gene="nuoL"
                     /locus_tag="VFH96_07035"
     CDS             17751..19853
                     /gene="nuoL"
                     /locus_tag="VFH96_07035"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET6973764.1"
                     /translation="MENNLLSLIIFAPLAGAAINWFIGRRVRDERFIGIVACGSVAIS
                     AIVAFYLALNSGGALRTETPVLDHIWTWLQVGNFHADFALGMDRLSGTYALFITFVGF
                     LIHVFAVGYMHGEAGFYRFFAFLNLFMFAMLTLVLADNFVLMFVGWEGVGLCSYLLIG
                     YYFDKKEAGDAAKKAFITNRIGDWGFVLGIMLTFFVTGSVSFFANPAAGEAAHTALSF
                     FRNQPVETFTAGAIFAGGATSIAVLLFIGAAGKSAQIPLYVWLPDAMAGPTPVSALIH
                     AATMVTAGVYMIVRCSEIFVHAPTAMFIVAIIGAATALFAATIGLAQNDIKKVLAYST
                     ISQLGYMFLACGVGAFIPAIFHVITHAFFKALLFLGSGSVIHGMHHEQDMRRMGGLKK
                     YMPITFVTMITGGLAISGVPFFAGFFSKDEILWRTWSAPAFSLPSTGFSKFLWFIGAL
                     TAFLTAIYMTRMMVMTFWGKERFRETHDSKHQEPDHHHGTVEPHESPWLMTVPLIVLA
                     VLSTVGGFIGIPYAFVGKDVNVIERTLEPVVPKAGAPVGTHATEAPVVEHGEAAATET
                     AHATHSSEELNAERMLAGLSVLIALLGIGAGWYMFQKRPLLQMPRLLENKYYVDEFYD
                     AALIHPIEVASREGLWKIFDMGALDGGLHTIGEAVQEMGRLARHLQAGFVRGYAAIIL
                     VGALILIGVFAYLGFPGV"
     gene            19893..21431
                     /locus_tag="VFH96_07040"
     CDS             19893..21431
                     /locus_tag="VFH96_07040"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET6973765.1"
                     /translation="MLTILILLPVAGTLALVGYSFVSSRREEHYRWIALIVTAATFVL
                     SLLLLRGIGASGAELRFEENVSWIGAIGSRYHVAVDGISLWLVLLTTLLMPIAVISSW
                     TAIHKRQLSYYAFLLILESAMIGVFVSFDLLLFYLFFEASLVPMFFLIGIWGGERRVY
                     AAVKFFIYTAVGSLLMLVGIISLYFLYHTFDYPTLLQAITATPLANFGRAEYWLFVAF
                     ALAFCIKVPLFPFHTWLPDAHTEAPTAGSVILAGVLLKMGTYGLLRFNLALFPNASRD
                     WAWVIIILAVIGIIYGALVAMVQPDVKRLVAYSSVSHMGFVVLGLFSFTELGMQGALY
                     QMLNHGVSTGALFLFVGFIYERRHTRQISEFGGLAKPMPWFATLFVFASLSSIGLPFL
                     NGFVGEFFILLGTWTSRAVGRPWLTTMLAATGVIWAAVYMLWMLQRVVFGPITNPENA
                     ELSDLNKREIGLLIPLLVLMLFMGVYPRVFLDRSKPSVEVIRARVSSSPAGGSYTTEV
                     VRQD"
     gene            21484..23082
                     /locus_tag="VFH96_07045"
     CDS             21484..23082
                     /locus_tag="VFH96_07045"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET6973766.1"
                     /translation="MSSFLAQSVMPIVNWSLIAPEVIVCAAAVLVMLVDAFVRPTQRW
                     ITGGISLAGIIAAAISTLWLWSTGTPSSDAFNGMIALDELRLGFTLIFLLVSALTLLI
                     STVWVQGEQLPAGEFHSLLLFATVGMMLMASGNDLVIIFLGLEILSIATYVMAGFRRT
                     DVRSNESSLKYFILGSFSSAFLLYGIALIYGATSIAEPGPGGSLSRIVAGTTNIAEIA
                     QRVGQAQYPALLFAGAAMMLVGFGFKIATAPFHIWTPDVYEGAPTPVTAFMAAGPKAA
                     GFASFMRVFIFGLPFVVSTSNATTGNLHHAWVSVLVVMAILTMTLGNVVAIVQNNVKR
                     MLAYSSIAHAGYAMVGFIAAGSATDPVQRHNAITSVVFYLLTYAVMNIGAFAVVQLIA
                     RNGDRRTSIEDYRGIGFESPVLAFSLSLFILSLFGMPLTAGFMGKILVFGAAIDQKFY
                     GLVVVGVLNTAVSAYYYLRLIIVMFFGERTMAWSAPRVPASVAVALVITVLGVLYLGI
                     FPGRVINALQTRIESQLFTNQLHR"
     gene            23082..23567
                     /locus_tag="VFH96_07050"
     CDS             23082..23567
                     /locus_tag="VFH96_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973767.1"
                     /translation="MPAESYAQLIQEQLGESWLPRIYRERILKLRTRSYHFESAKPAA
                     RVEIQHTLLGVELKVGRRRLLCPDLATARYLSVFARLGCEDVAVPYDITKISHLADEL
                     ESSWYRMFLLVEQETRAKSTRFSARLRGLLIAKIREEIKEAGPGTRVPEFRQTTKQRT
                     R"
     gene            23612..24493
                     /locus_tag="VFH96_07055"
     CDS             23612..24493
                     /locus_tag="VFH96_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973768.1"
                     /translation="MTQEYTPTTSLPFPFNQQNFCRYEPINQSIDEARVLVIDNLLRD
                     ERDLSSVARAEWGETLAAEMRRERLIAGMAVENILNNISRLVKTPTTEVAHLSEVGTA
                     AKSFAPDAIVLSGTLRDFDYYDQSILDDFGQFIRNTKTPVLAICGGHQLVGLSFGARV
                     ITLDKLEQHEQRLARPQEYQYRFIRITEPDDPIFKNINCSEPDAPARILRVWQNHGLQ
                     LDRIPEGFELLATSYLCRNQMMVKRSDGQMIYTVQFHLEKSFEDWNKSRTRWQHPNDS
                     RDGRILFENFLTLAVQN"
     gene            24490..25167
                     /locus_tag="VFH96_07060"
     CDS             24490..25167
                     /locus_tag="VFH96_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973769.1"
                     /translation="MRPLTEKTLAVAARELATRDEMLAGIHAIHGDPPLWRRATGFTT
                     LVHIILEQQVSLQSAKAMLVRLQGIIEPFTPERFVELGYDYLRSLGVTRQKSAYLIDL
                     STSIVNGQLSFTKLARMSDDDARLALTRIKGIGLWSADVYLLMAMRRADIWPAGDLAL
                     AVAMKELKGLPVRPSSLELEQLAEQWRPLRAVAARMLWQYYLAKRGTQKSTKEQKAQK
                     ESSEFKL"
     gene            complement(25215..26378)
                     /locus_tag="VFH96_07065"
     CDS             complement(25215..26378)
                     /locus_tag="VFH96_07065"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HET6973770.1"
                     /translation="MFDQFTLGIEEEFQIVDPQTRELRSHVAEILEEGKMLLGEQIKP
                     EMIQSMVEVGTGICRNIQEARTDITRLRSIIAGLARKNGLVIVAASTHPISRWQDQKI
                     FDDERYELLVQELQTVARSLLIFGLHVHVGMADRERAIHIMNAARYFLPHVLALSTSS
                     PFWMGHNTGLKSYRTEIFKQFPRTDIPDHFDSYLGFQRYVDLLVKTGCINDGKKIWWD
                     IRPHPYFPTLEFRVCDIPTRVDDTIAIAALFQAIVAKLNKLIDQNLGFRLYRRMLIQE
                     NKWRAVRYGLDGKLLDLGKQTEVPVRDLVMELLEFVDEVVDELGSRKEIEHIHTILER
                     GTSADRQLQVYHETNDLKAVVDDLIKLTMENVPDTPDLTIPPQTTTTASTIAK"
     gene            complement(26496..27014)
                     /locus_tag="VFH96_07070"
     CDS             complement(26496..27014)
                     /locus_tag="VFH96_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973771.1"
                     /translation="MAEVELTWADLSGETREMLSGKLAGLWGAESDAAAFDSWPLDKK
                     HALLLLLKRMDTKKLWHLVKRITNVYGEGGVGLQFEAWPMIESTLSRRNDFTRLMANH
                     KDTTGGFYEKRRAAAVLHFLFQEGEPRTWYVHFDLYSPVHSPGSMWKHLRHEFMGKVK
                     PDWKMIAKWLDK"
     gene            complement(27007..27342)
                     /locus_tag="VFH96_07075"
     CDS             complement(27007..27342)
                     /locus_tag="VFH96_07075"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6973772.1"
                     /translation="MPDDKDRGFETQEERRSYNRSRLIVDVFFDGKDLTGVASTKDIS
                     VGGLYMNTQAEIPEGTLLLVRIPFRHDVQAVCNAVVVYSNPGRGVGLRFQGLSDDVRA
                     MLEREVSNG"
     gene            complement(27394..28746)
                     /locus_tag="VFH96_07080"
     CDS             complement(27394..28746)
                     /locus_tag="VFH96_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973773.1"
                     /translation="MFPEAVARYHDLLSNQELAESSRALLDEGLEKARLIFGGRRLSP
                     YLRPHFVSESDFAHICQICETVWSAIQKVKDAAVADPNVVDDLGLTEIERELVAIDPG
                     YVAVSPTSRLDSFLTKSAYSFVELNGESPAGIAYADAAYDIFSSLPVMREFAVDYNVR
                     PLYGRRLMLDVLLSSYEEFLGRKPERPPRIAIVDLADVPTVKEFELFREFFEQSGYPS
                     IICTPQELEFSNDRLSARGQEIDIVYKRLLVNEYLPIIKEYPALLDAYRAGAICMVNS
                     FRSKLIHKKALFAVLTNPKHAPLFSQQELDAIHAHVPWTRVVRDEHTDYFGREVNLLD
                     FIRADGERLVLKPNDDYGGHGITIGWNVDQRGWDEAINNALADGDYLVQERVPTARET
                     FPALTDDGRIEFAEQLVDLDPLLFNGKVGSAFTRLSFTELANVSSGGGMVPTYIISKK
                     "
     gene            complement(28804..29097)
                     /locus_tag="VFH96_07085"
     CDS             complement(28804..29097)
                     /locus_tag="VFH96_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973774.1"
                     /translation="MTRKIIYAFSKRAFLNPASTHLTSYIQAYVQRGDKWGDNTVLIA
                     DCKRVVQLEFFLGTKRHRRISLAKIDLLIEILTQFRAALVKEIQSIESQKGTK"
     gene            complement(29263..29466)
                     /locus_tag="VFH96_07090"
     CDS             complement(29263..29466)
                     /locus_tag="VFH96_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973775.1"
                     /translation="MNKLRRMCAVAIVTAVLAVSAFGGQLDSPGVIGTIDSPGAPAPQ
                     PTQTSTSTSTTTTIILTILSLIR"
     gene            29615..30937
                     /locus_tag="VFH96_07095"
     CDS             29615..30937
                     /locus_tag="VFH96_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6973776.1"
                     /translation="MTLRASLLRELVNSNLDVGGRAELCCKLAREFENKGEYEEAREM
                     LYGLWPRIDQRPRVKGLEPGIAAEVLMRAGVLTGWIGSEQEQAKDFISESLTIFEFRK
                     YKKKTAEAQTELALCYMRLGEYDNASDLLKLALRELITDSELKAKALLRLGIVKRHAS
                     PLNEALTFLTNNESLFHKINNQTLKGCYHQTIGDVLENLCESVGPKDYLDRALIEYAA
                     ASHHFELAQHRYYLANVENNLGFLLYKVDQFEEAHEHLNRARRIFTCLKDRIALAVVD
                     ETRARVFLKEKRNAEAEKAARSSVQTLESRDRPSLLAEALKRHGMALARLGHYSAALN
                     AFRRAIDLSQDNFNRAADIALTAFQELGDRLAVKDAITTSGRPLNEEIFSLEHSLIKH
                     ALENAHGRVTFAARTLGMSYQRLTHKLQTKHKDLLKDRSPLRHRTRKP"
     gene            31080..32063
                     /locus_tag="VFH96_07100"
     CDS             31080..32063
                     /locus_tag="VFH96_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973777.1"
                     /translation="MSEDIEIEDASGRLGSITFDRSPRTLTAERTASGFELQLPIEIT
                     LRVRLRDDPMLMLSNLRGSVLVKKPDGSPINVGTLLGKFRYTAGISTSEPYSYKNTKY
                     LSWTGTFADLAHIEQCRNGQPPQFLMHVEGEWSFLVPPVLPSASEIPQELLSQELETR
                     LRKYQSFRFLTEPQDVYSRTGNIQVTYPRELWVEMLRRLGVAENVLVEVPLPANPSGP
                     WDDVWQALIDARNAFEQGGSTGWQSTVTSVRRALERWHEIEQEDQGPAWKTPSQSDLK
                     LRTKKQRLDALRWHLRQVAHLAPHSSAEEWTRDDALLMLATLSSLLAERKP"
     gene            complement(32099..33637)
                     /locus_tag="VFH96_07105"
     CDS             complement(32099..33637)
                     /locus_tag="VFH96_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6973778.1"
                     /translation="MNFRRIISAVLLVSVLLCQAIATAQTPAPSPAPAPKAPKAPAAP
                     QVVTIIHRLNGLKMFRLLLRSEQVQSIDALDSAFNLMDDVHTNVIAGLAMDDGETIAA
                     WLPEADVEFGPPVVAFPVRPGPPKSPRAATGMFPGAVNGFFDGPDVSVIGPDGKQIPA
                     KYIGLDAATGLSILRLENKSAVSASAIKAEQVSSGDVVLLFGPEPVTKTRPVLARMAA
                     IEGRVEDVVPAPSGGIARFRVSAAKLSKSNIGGVAINQAGDTIGIIDGLEGNEASILP
                     AASIKRAAQRVLERQASVPRPWLGVKGEAVAALKLDQIQNHGWEMTRAAALAGKHRGI
                     LLTSIVPGSPAERAQLRAGDVILKVDDKDIETADEFTWWLDQAGPSSSVEFTVTRPDR
                     PMEWAMNVKLSGMSDQWSRVNWRSGFPSMKGFWLIDQGIETIALKPLVASQLGTNPGL
                     LVIYVDPVTAAFEAGLEPGDVIQTINGKPVSAFRTTPNSDSYTFEVVRKKQKRVVKVP
                     AKKK"
     gene            complement(33649..34380)
                     /locus_tag="VFH96_07110"
     CDS             complement(33649..34380)
                     /locus_tag="VFH96_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973779.1"
                     /translation="MRKTNCKVIRRELDELMLGESWSAAATGHLHECAECREFHVQQT
                     KLRQIVGSLGTVEAPADFDFRLRARLANDASSGPAIYWRLVRGGLVTAALAIIVLMGV
                     VAVRDAMRLGENNGEVARKPEVHQPVKTDQAPPVVQEDKKPDQVVAGVTTAPIRKIRP
                     TQIDYRRQPLSTKDVSSVGASVLNGTTPAGMSAAFPIDSSVQSFTVSLEDGRGNAKTI
                     SVPTVSFGSQRIGPGGNQFAPKRDW"
     gene            complement(34393..>34715)
                     /locus_tag="VFH96_07115"
     CDS             complement(34393..>34715)
                     /locus_tag="VFH96_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6973780.1"
                     /translation="RKRKRRKLVSLTGFFQQTDGGEAKELNPPDGRPLQDLELVDTER
                     RNAVQRAIGTLPEKYRAPLILRDVDGRSYDEIAKILETSEGTVKSRISRARGFLREKM
                     RAYL"
BASE COUNT         7427 a         9552 c         9714 g         8022 t
ORIGIN      
        1 aaaggtgtcg acgcgaagaa gacgctcgat tcgatccgtc tgctgaaaga cgcgggcgtt
       61 gacggcgtga acattcctga cggcgcgcgc gcgcagtcgc gtatgagtgc gctggcgacg
      121 gccgtgctgg tcgaacaaca gatcggcatt gaaacggtgc tgcactactg ctgtcgggat
      181 cgcaatctgt tgggcatgat gtccgacctg ctcggcgcgg cggcgctcgg tttgcgcaat
      241 atcctcgtga tcaccggcga cccgccaaag atgggtccgt atcccgacgc cacagccgta
      301 ttcgacatcg attcgatcgg cttgaccaac atggtcaaca aactgaacca cggtctcgat
      361 ctcggaaaca atccgatcgg cgagccgacc tcgttctgca tcggcgtcgg cgtgaacccg
      421 ggcgcgatca atctcgacga agagatcagg cgcttcgaat ggaaggtaga agcgggcgcg
      481 cagtatgcga tcacgcagcc tgtatttgat accaatcaac tgcgcgattt tctgaaacgc
      541 atcgagcacg tgcgcattcc gatcattgcg ggcatctggc cgctcgtgtc atatcgcaat
      601 gcggagtttc tgcataacga ggtgccgggc gtgcgggtga cgccgtcgat catggaccgc
      661 atgcgtgcgg cttcaaacat cagcaaggaa gcagggcgtg acgaagggtt gaaaattgcg
      721 cgcgagtctc tgctggaagt gcgtgatgag attcagggtg tgcaggtgtc agcgccgttc
      781 ggaaacgtca agtacgccct ggaagtgttt gaggccctgc cagaatttag ccgcggataa
      841 cacggataaa aatccggatt ttatccgcgt ttatccgcgt tcaaaagatg ccttagtgcc
      901 gaatcaagcg tgggataagc gaaattttgt aacacgcccg gcttcacccg cgcgctcgat
      961 aacaacagcg catcgcccat ctcaccaaac gcgagccgcg cgccgaacgc cggtatggga
     1021 aatatggtcg gcttcgaaag cacgcgtccc agcgtcttcg taaattccgc attcgtcacc
     1081 ggattcggtg cgacaaaatt caccggtcca tttactgagc tgttcccgat cagaaatttc
     1141 aaaccattaa caacgtcctg aatatcgatc caactcatcc actgcttgcc atcaccgatg
     1201 cgtccgccaa tccccatccg acaaggcgtt agcatcttgg ccagcgcgcc ttccttctga
     1261 tcgagaatga tgccgaagcg cgcgtgtacg gtcctgattc ccttctcgat cgcgggtatc
     1321 gtagcgtttt cccactcgac acaaacattc gctaagaagt cgtctccggg cgcgctttgt
     1381 tcagtcaaca cttcatcacc acgattgccg taataaccga ttgctgacgc gctcacgaac
     1441 actgacggcg ggcgtgacat tcgcgcgagg gcctcgctca agagcgcggt tcctttgacg
     1501 cgactgtcgc gaatcgcacg tttcttctct tcggtccaac gactgctcgc gatgctttca
     1561 ccggcgagat ggaccaccgc gccgaggcct tcgaggctcg cggcgtcgat cttttcctga
     1621 ttcggatgcc actcgatttc cgtcgcgccc gccggcttgc ttctcacgag acgaacgatt
     1681 tcgtgaccat cagacgtcag cgagctgatg agtgcctggc cgacgagtcc atgtgaacca
     1741 ctcacgagta ttctcattgg ttttctcgcc gtgagaataa catcaaaaat gtccgataaa
     1801 cttcagtttg tcgcgcaatc ccacaagcta aagcatgtcg gacatcaaac tgcacttgaa
     1861 attttttaat atccgtgtag aatgcgcgga aaaatttaag taaggagcaa cctcacgaat
     1921 gcttcgtctc acgacgtttt tcctcatctc cgtttttact ttcatcgtca tcgctatttg
     1981 tgggccggga cgtgtgcggt cagtttcaac actgacgcgg ctgacgaata cgccggaaca
     2041 cgtgctcaat ctcaatccag ctattagtga tgatgggcgc acgattgtct ttgagtcgtc
     2101 tgccgatctc gcgaatacgg gcggaagcgc ttcgtttcgc ctgttgcgtg ctgatccttc
     2161 gtttgttgag ctgggcagga cgcgcgcggt gtgtccggcg atgtctggtg atgggaggac
     2221 aatcgtcttt gcgtcgcgag aggatctggt cgggagaaat gcggatcgga attcagagat
     2281 ctttctgttc gatggtgtga agctaaatca gttgacggag acatcaggtg cttcaaacct
     2341 ccaaccgtct atatcgagtg atggtcgcgc ggtggcgttt gtgtcgaatg gagagatcgt
     2401 tctttatgac acgttggacc ggaagtctac gcggttgggc agcggcgtga gtccgaagat
     2461 tagtggtgat gcccgatatg tttattacac gaggaattta aacgcggata acgcggatct
     2521 aatacggatt gatgtggaga cactaacgag ccgagttcta ctggcgaatg tggctgagtt
     2581 atcgattgtt gaggggcggg ctgtgagtaa tgatgggatg cgtgtggttt attcggcggc
     2641 tacggggctg aaccagacgc aggttttcat gtttgatggg cgtgatgagt cgattaggca
     2701 gttaacaaat ctgggatcga gggcggttga cgttgggttg cagccgacga ttagcggtga
     2761 tggacggcgc gtggcgtttg cgacgcgaag gcgggtagtg gggttgagtg atggtggcgt
     2821 tgagctgtat gtgcttgatc tgcctacggg ggtcatacaa caaatcacga atgcgccttc
     2881 tacagcgact ggtgaagtgg tttcatcgct caatttcgat gggagtgcgg tggcgttcaa
     2941 ttttccacgc atactttccg gggcggtcaa tgatgacgat ctgagaaata acagcgagat
     3001 ctatgtggcg tctatagcgc cgcgaccaat tggaacagcc actgtagtaa atgctgcggc
     3061 gcctgagttg gcaaacatag cactcggcag cattgcgagt attcgcggga gtgcactcgg
     3121 cccttctgtg gccgtcaatg gccaggcggc gcaaatcttt tattcctctc cacaagaggt
     3181 cgtgtttctg gttccgagtg gattgatcgt tggcccggcg cagttcctgg tcaccaatgc
     3241 agaagggttg cagtcgaagg ctgaagttat catttccccg acggcgcccg gcgttttcaa
     3301 taatgccggt gaagcgatcg ttttgaattc cgacacgctg acgccgggac cgtttgatcc
     3361 gtcgaaagga gagttgcgac tgtcgatttt tgtgaccgga gtcgcgcgag cgggcaatgt
     3421 attggtgacg attaatagca agcccgttat cgtcgagact gtggtaccgg cgagtttgcc
     3481 gggactcgat gagattcacg tgctcgtacc tgctgaattg cgtggcgcag ggaaatcaac
     3541 actcgtcgtg tcagcggatg acgtacaaag caattcggtg tcactcgtac tgacgggggc
     3601 gacacccacg cctactccga ctccgactcc gacgccatca ccatcgccga gtccttcgcc
     3661 aacaccaaca ccgtcacctg gttcatcgcc gcatatcgtg atcagccagg tcttcggcgg
     3721 tggcggtaac tccggcgcac cgtttcgtaa tgacttcatc gagatcttca atgcgggcca
     3781 gacgccggtt aatctcagcg gctggtcggt gcagtatgcg agcgcgacgg cctcgacgtg
     3841 ggcagtcact ccgctaactt ccgtcactct taatcccgga cagtattacc tgatccagga
     3901 aagctccggt ggcagtaacg gcgttctgtt aacggcgccc gatgcaaccg gcacgattgc
     3961 gatggccgcc ggctccggaa aagttgtggt caccaaaacc tcgactacac tgacaggcac
     4021 gtgcccgaac gatccgaaca tcgtcgacct cgtcgcctat ggcaacaccg ccaattgttt
     4081 caaaggctca gctcccgctc ccacgccgag caacacaaac gcgatccagc gcgccgcggc
     4141 cggttgcaca gacacgcgta acaacgtcgc agacttcgct ctcggcccgc ccaacccgag
     4201 aaattcaaat tttttgcctc gaatttgcac cgatccgtaa gatccgtgtt atagactcgc
     4261 acgcatgcac tcgctcacgg acgcagtcgc ttcgctccgc cagcacttcg gcttcgaaga
     4321 ctttcgcgaa ggccaacgcg aagtcatcgg ctcgatcctc gaaggcaaag acgcggtcgt
     4381 cgtgatgccg accggcagcg gcaagtctct ctgctatcaa ctgccggcga tgatcctcga
     4441 cggcgtcaca ctcgtcgtgt ctcctctgat cgccttgatg aaggatcaag tcgacgcatt
     4501 gcttgctcgc ggattgccgg cgacgttcat caacagctcg atctcagaat cagaacaacg
     4561 cgcccgcatc gatgcgctca aacgccgcga gcacaaactc gtctacatcg cccccgagcg
     4621 ctttcgcagc agccgtttca acgcggcact acaacaaatc cccatctcgc tcttcgcagt
     4681 cgacgaagcg cattgcattt caacctgggg ccacgacttt cggcccgact acctgcgcct
     4741 gcgtaacgtc atcaagtcgc tggacaaagt ccaaactctc gcactcacgg cgacggcgac
     4801 gccatacgtc cgttccgaca tcattcagca gctcggtctc gatcgcccgc aaacgtttgt
     4861 cagcggtttc gatcggccga atctctcgat cgaggtggac cacacggagc gcgagcgcca
     4921 gaagatcgcc cgcattcgca acctcgctca ccaaaatccc ggttcaggaa ttatctatgc
     4981 ctcgacccgg aaagcagtgg aacaggtcgg cgcaaagctg agggcgctcg atctcaaggt
     5041 gtcggaatat cacgcgggca tgagcgacgc cctacgcatc cgcgcgcagg aagatttcat
     5101 gagcggccgg acgcagatga tcgtcgccac gaacgcgttc ggcatgggca tcgacaaacc
     5161 tgacatccgc tttgtggtcc attaccagat gcccggctcg atcgaagcgt actaccagga
     5221 gatcgggcgc gccgggcgcg atggtctgcc gtcgacgtgc gtcctgctct tcaactacgc
     5281 cgacaaaaac acacacgact tcttcatcga aggctcgtat ccaaacatca gcataatcaa
     5341 acaagtttac gactcactcg cggggacgga gctcaaacga atcgagctct cgacgcagga
     5401 gatcgcgcgg cgtgccggtg aaacaaacga gatggcggtc caatcggcgc tttatatgct
     5461 cgagcgcgcc ggacacattc aacgcacggc gcccgcactc aatcgcggcg gccgtggcgc
     5521 gcaacaaggc cgcagcatca tcatgctcga taatgagccc gtcgcgaagc tgcggatcga
     5581 tccggcagag gcgtcgcgtc gtggcgagct ggagcggcgc aagctacgag cgatgatcga
     5641 cttctgctac acggatcgtt gttatcgtgc gtacattctt aattacttcg gtgatcgcaa
     5701 aagcgagcgg ctgtgtggga cgtgcggcaa ttgcgaacca acaagcacca catcaattcc
     5761 atcagcgcct gttgatttcg cgccgcgcac accaacggac gatgaattac tgcgcgtgcg
     5821 gaaaatactc gcatgcgcga aacggatgaa tggtcgcttc gggaagaaga tgttgatctc
     5881 gacgctgcgc ggatcgtcgg cgaagcagtt attgacggcg aagcttaatg agttgtcgac
     5941 ctatggattg ttgagagaca tggcgaacga cgagttggag gcctacatcg atgcgttgat
     6001 tgaagcgcat tgcttgcgcg taaaacccgg cgcgtatccc acaatatcaa cgaccgaact
     6061 cggcgatcgc gtcatgcgcg agcaggagca gatcgcgctc tcactaaaat ccgtgtaatc
     6121 cgtggccgga ttttcttact tgatcacgcc ctgcaacgga ctcgacgcgc tggcatacag
     6181 acgcttcgtc atccgtcccg caacatacgc cagtcttcca gcctcgatcg caagcttcat
     6241 cgcggtcgcc atcttcgaag gatcttcagc ctcggcgatc gcggtgttca tcaacacacc
     6301 atcagcaccg agctccatcg cgatcgcggc gtcactcgcc gttcccacac ccgcatcgac
     6361 aatcatcgtc gcatcgggca attgctcgcg catgatgcgc aggttcgccg gattttgaac
     6421 acccaaaccc gatccgatcg gcgccgcgag cggcatgatc gctgcacaac ccgcgtcgag
     6481 cagtttcttc gccatgatca ggtcgtcgtt gaaataaggc agtacgacaa aaccttcctt
     6541 cacgaggatg cgcgcggctt cgagtgtctg ttcgttatcg ggaaacaaag tcgtctgatc
     6601 gccgatgacc tcgagcttca cgagatccgt ctgcaaagct tcgcgtgcaa gccgggcggt
     6661 tctcacggcg tcttcagcgg tataacagcc cgcggtgttc ggaagaatct gaagtgcagg
     6721 actcaggtaa tcgagaaacg actcgctact gcgatcgagt ggcacacgcc gcacggcaac
     6781 ggtcaccatt tcagcgccag aagcttcatg tgcgctgcgc atctgttcgt agctgcgata
     6841 cttgcctgtg ccgataatca gtcgtgatga aaactctcgc ccggcaatga tcaatttctc
     6901 ttttggcgat gctgcggttg ctgctgtcga catttatcct ccaccgacga agtgcacaat
     6961 ctcgatccga tcttcgtcct tcaacatagt actgccccaa tcactacgac gcacgacgtc
     7021 acgattgagt tcgatcgcta tccgcgccac gggcagatcc agctcagcga tcaactccgc
     7081 taaagtagaa acctcaactt cccttagctc atcgttgacg aagactttca ttagccacaa
     7141 aaaagcacaa aaagcacaaa aaagctaatg cgcttctttt tgtgcctttt gtgccttttt
     7201 gtggctaatc ttttgggtgg atgaggcggc ggagctcggc ggcctggtct tttgttaccg
     7261 cgcgaattaa cacggcggcg cgaccggagt tgaactgcgg aatgtctgtg ctaatgctcg
     7321 ccactccatc actctcagtc ttcaacgtgt aaatcaacgg ccggaacgtc gtgcccagca
     7381 cctttactga cacagctacg ttcgcaagcg gcttctcgtc gccattcgtg cgattgttaa
     7441 ctaagactcg caacgtataa cgctgacctg agtaaaactc atcgtcatcg agcagctcta
     7501 taaataagcc atcttccaga tcaggctcac tcacaaactc ctcaacttcg agcggctctt
     7561 caacctccgg ctcaggttcc ggtgccttcg ccgtcagatc caccggctca taacgcggct
     7621 ccatgccggg ctccatcact gccgcgagct cttcatcctc cggcgtcggt ggcggcgtcc
     7681 actcctgcga caccggccag tactcgatct caaacggctc atcgaccgtc gacacagatg
     7741 gcgcctcgac ttcaggcgca gcttcctcca cagtcttttg cggcgcttct ttcgggcgcc
     7801 cgctcatctt cttgagatcg tcgatacgtc ctgaattgat cgcggcgcag atcaaccggt
     7861 gctgccgttt gagccgttca gagagaactt cgtcactaaa acctgacgcg atcagatctc
     7921 cataaggaga gcgcttgctg gccaggatcg tgccgcccac atagacgagc gagaggatga
     7981 ttggtgagtc gagccccttg tcctcggttt gcacatgata ggtaacgcca tcatgctcga
     8041 tatcggtgtt gaatcctgta atcacagttg tcagaaccgg gcggggttcg cgccggggat
     8101 gttccgcaag acgagtcctt tcgacttcaa accgggaggc tgctcaaacc tactcgcaag
     8161 caggttttac tgaacgttaa cacaagcatt ttttcacggt caatgagcat ttttcccagc
     8221 ctgatccacg cgagaagcgt tgcttgacac ccttgatcag ttcgactagc atcacgcttg
     8281 tacaatgatt ctcaatctcg tggaccgagt gacactgacc gcagacgatt gctcagcggc
     8341 cggtgatagt gtgcgttaga tgagttttcg tctttacgat tacctaccca ttgtgctgat
     8401 gttcatcgtc gcaggcggct tcgcggtggg caacgttctc ctgtctcagt ttgtcggaca
     8461 acgtaaacga acaagaacga agttgatgcc ttacgagtgt ggtaaggacc cggtaggctc
     8521 cgcgcgcgag cgtttctcgg tgaagtttta cctgatcgcg atgatcttca tcctgttcga
     8581 tatagaagtg atctttctcg taccgtgggc ggccgtgtac aaggcactcg cggcgcaggg
     8641 cccggcaatg cgctcgtttg catatctcga gatgatgatc ttcgtcggtc tgctttttgt
     8701 cgggtacatc tacatcgtca agaagggcgc gttcgactgg ggcgagagag cacgacgcga
     8761 agcagaagcc gaagcaaggg ctttggccga tctggaagca cgaaaggttg agaagcggcg
     8821 acgacctgct gcataaggaa gtttaaatgg gtctcgaaaa cgtaattgcc gaagctttac
     8881 cggaagtgct gaccacgcgg ctcgattccc tgatcaactg ggcgcgcaag tcttcactgt
     8941 ggccggcgac atttggtctt gcttgttgtg cgatcgagat gatgaatgcg acttcgtctc
     9001 gaaacgatct cgcgcgtttt ggctcggaag tgtttcgcgc cagcccgcga caggctgacg
     9061 tgatgatcgt ctccgggcgc gtatcgcgaa agatggcccc cgtgctgaga cgaatctacg
     9121 atcagatgcc ggaaccgaag tgggtaatct cgatgggcgc ctgcgcgacg tccggcggtg
     9181 tttttgataa ctacgcaatc gttcagggtg tcgacaagat cgttccggtt gacgtctaca
     9241 ttccgggttg tcctccgcgc cccgagatgt tgatacacgc cgtgatgatg ctccaggaca
     9301 aagtgatgaa agagagcgtc cgcgatcgcc gggacattcc cgaagaggaa gcacgcgaag
     9361 ccgtcgtgcc cggcactttg ccgatcaccg atggctcgac cgcgcaacaa aacacgttgc
     9421 cggaatcgcg gccctacacg atcgcttcga gacacgaacg gcgtcgttct acttagagag
     9481 tcgagcaaca agcgcggatg acaacagaac aaaccccgag ttcagaaata gcgccgtcac
     9541 cgtcagtgac ggcgttacag cgtgaacatc ccgaatgggt gcgcgaagtg atcgtcgctt
     9601 tcggtgagac gacgatcgtc gtgccacgcg aaaacatcgt cgatgcttgt ttgtttttga
     9661 agaattcacc ggaaacacgc ttcgactttc ttgcagatat ctgtggcgcc gatcgcggtg
     9721 tggaagaaga gccgcgcttc gaagtcaact accacctttt ctcaaccact aaataccatc
     9781 gcgtcagatt aaaggtgtta ctgaacgagg aagacacgca cgtacccagt gtcacgggcg
     9841 tctggcgcac gtcaaactgg cacgagcgcg agaccttcga cctgttcggc gtgatcttcg
     9901 acggccatcc ggatctgcga cgcattttgt taccagacga ctggcaaggc tacgcattac
     9961 gtaaggactt tcctttacgc ggctacgaac cgtacagcct gaaataactg atcgtgtatt
    10021 tttccgtgta atccgtgtta actgatttat gagctcagta accattccac cacaattcga
    10081 aatcgtcgat cgcccgctcg acacgcagat gacgatctcg atgggtccac aacacccgtc
    10141 gacgcacggc gtgctgcgtc tcgaattggt gctggacggc gaaatggtcg tgaaagcgac
    10201 gcctgacatc ggctatctcc acacgggcat ggagaagctg tttgagtaca agaagtacca
    10261 gcaaggcatc gtcatcaccg accgcatgga ttacctcaat ccactcggca acaatctcgc
    10321 ttacgtgatg gccggcgaga aactgctcgg tctcgagatt cccgaacgcg cgcaaacgat
    10381 tcgcgtgctg atgtgcgaac tacagcgtat cggttcacat cttgtctggc tcggaactca
    10441 cgcgctcgat atcggcgcga tgacggtctt cttttactgt ttccgcgagc gcgaaaagat
    10501 cctgaacctg attgaagccg cttcaggtgg acggctgacc ccgtcgtact tccgcatcgg
    10561 cggtttaatg atggacctgc cggcgggttt cgataatcgc tgcaaacagt ttctcgacga
    10621 cttccctccg gcgctggatg agtttcataa gcttttaagc ggaaacaaga tttttcagcg
    10681 acgcacgcag ggtatcggac gcatctccgc tgaagacgcg atcgattggg gactgtcagg
    10741 tccatgtctg cgtggcagtg gtatcaatct agatatacgt cgcgcgaacc cgtacagcgg
    10801 ttatgagaag tacgattttg aagtgccgac tgagcctgat ggtgatgttt gggcccggta
    10861 catggtgcgg atgcgtgaga tgaaggaatc gcacaggatt gcgacgcagg ctttgtctcg
    10921 tttgcagcct gggccgatca aggctgacgc gccgaaagtc gtgctgccgg atcgtgaagc
    10981 gatgaagagt catatggatg cgttgattca tcacttcctg atcgttgcgg aaggtttcga
    11041 cgtgccggcg ggtgaggttt atcacccgat tgaagcttcg aagggcgagc tcggtgtata
    11101 catgaggtct gatggtggac caaaaccgag tcgagttcac ttccgtggtc cgagctttgt
    11161 gaatttgtcg gcgctgccgg tgatgtctga aggcgagatg gtggcggata tcgtggcgat
    11221 cattggatca ctggatatcg tgttgggaga aatagatcgt tagccacgga ttacacggat
    11281 tttcactgta aaaataatct gtgtatatcc gtgtaatctg tggctaaaac ttcttagatg
    11341 agtacgcaaa tagtaaacat tcaaccgttg tcgcagcctg tcgatttggg ggttgagccg
    11401 gcgacgtttt caccgcaagt ggaggcggag atcgatcgac acctcgcgaa gtatccggtg
    11461 atgcgctcgg cgatcctgcc gttgatgttc atcgtgcagc gcgagcgtgg ctatctcgat
    11521 ccgccgggcg tgctttatct cgcgaatcgt ctgaaggtgc gcgtcacgga catctgggaa
    11581 gtcgcgacgt tctactcgat gatccacacg gagccgatcg gcaagtatca cattcaagtg
    11641 tgcaagacgc tctcatgcaa gatcatgggt gcggggaaag tcaccgaaca ttgttcgcaa
    11701 aagttgggaa tcaaagtcgg ggagacgacg gctgatggac gcttcacgtt gagtgaagtc
    11761 gaatgtctcg gctcgtgcgg cacggcgccg atgtttcaga ttggcttcga ctatcacgaa
    11821 aacctgacgc cggaaaagat cgatcaaata ctggacaaac taccttaatg cgtatcgcgg
    11881 caatcggatg tgaaccatta caactgcgcc gagttcatct cgagtggtcc cacacactcg
    11941 acgtctatcg tcgcaacggt ggttaccaag cgctcaaaaa agttctggac ggcatgtcgc
    12001 ccgacgacgt gatcaacgaa gtgaagaaat cggcactgcg cgggcgcggc ggcgcaggtt
    12061 ttccaaccgg aatgaagtgg agcttcgtgc cgaaagactc accgaagccg aagtacgtcg
    12121 tctgcaatgc cgacgaatcc gagccgggca cgttcaaaga tcgctacctg atggaacgtg
    12181 atccgcacat gctgatcgaa ggcatgctga tcgccgccta cgcactgaat tcaaaaacct
    12241 gctacatcta cacgcgcggc gaatataaat atttgatcga catcatggac gtcgcgcttg
    12301 aagaagcgcg caaagcggga ctcgtcggcg acaagattct cggcactgac ttctcctgcg
    12361 agatccacac gcacaccggc gcgggcgctt acatctgcgg tgaagagacc gcgctgctca
    12421 gctcgctcga aggcctgcgt ggtcatccgc gcatgaagcc gcccttccct gctgtttccg
    12481 gtttgtatgc agcgccgact gtcgtcaaca acgtcgagac actcaccgcc gtgcccgaca
    12541 tcatcaagat gggcggcgag gcttatcaaa aactcggcac tgagaagagc ggcggcacga
    12601 agctgtggtc cgtctccggt cacgtcaagc tgccgggcgt ttacgagttg ccgatgggct
    12661 acgacgacat ggaaaagttc atcatgcaag actgcggcgg catgctcaac gacaagcagc
    12721 tcaaggcttg cattccgggc ggctcatcgg tctacatcat gcgcgccgat cagattatcg
    12781 gaaaagacgt gcgcatggat tacgaggggc ttgtcgcagc gggttcgagt gtgggatcgg
    12841 gtggtttcat cgtgatggat gagacggtcg acgtgtttga gtcgacgaag aacctgactg
    12901 agttttacaa acacgagtcg tgtggctggt gtacgccttg tcgtgaaggc acggactggc
    12961 tggtgaaaat attcaaacgg atcgagaacg gcggcgggcg tcctgacgat gcgcaactga
    13021 tgctcgatct cgtcgacaac atcgagggca agtcgttttg cccgctcggc gacgcggcgt
    13081 cgtggcccat tcagtcggcg atcaagagat tcccggaaga tttcagaaaa aggctatcca
    13141 cagattacac agattaatct gcgtcatctg tgtaatctgt ggataaaact gcatgtcaac
    13201 tgaacaggtc aaagtaacta tcaacggcaa agatttcgag gtgccgaagg gcgcgcgtct
    13261 gatcgacgtg tgccgcgaaa aaggtttcga tattccatcc ttctgttatt actccgatct
    13321 cgcgctgcag gcttcgtgtc gcatgtgtct cgtacggatc gagaagatgc cgaaactgca
    13381 aacgtcctgc acgatcacct gcactgacgg catgattgtc acgacgcaga gtgaagagat
    13441 cgaaaaagca cagcgtggga tgacggagtt tctgctcgcg aaccatccgc ttgattgccc
    13501 tgtgtgcgat cgcggtggtg agtgtgagct gcaggagatg acgttcgact ggggcggtct
    13561 cgaagaacgc ttcaccgaaa agaagaatta ttacgccgag cgatatctct cgccgatggt
    13621 agcgaacgat ccgcagcgtt gcattctctg taaacgctgc acgcgcgtgt gcgacgagtg
    13681 gatgggtgaa gacgcgatcg aagcgggcgg tcgtggcgcg cacaccgtta tcggtacata
    13741 tctcggctgg ctcgattgtt cgcagtgtgg gaactgtatc gaggtctgtc cgaccggaac
    13801 gctgctcgac gcgacttatc gacaccagac gcgtccgtgg gagctcgcgc aaacgatcac
    13861 gacgtgcaac ttctgttctg atggttgcca gatgtcgctg ggatcgcgtg gtggtgaagt
    13921 gcggcgggca gtcgcgcgcg atcgttacgt caatggactt aacggcgagt ttctttgcgt
    13981 gaagggacga ttcgggcatc cgttcatcaa tcacgaagat cgcattcgca cgcccatgat
    14041 ccgctacaag aaaggcggca agctgattcc ggcaacgtgg gatgaagcga tccgtcacgt
    14101 cgcggagcgt ctcgaccagg ttgcagatgc gcatggacga aactcgatcg gtgtggttgg
    14161 gagtccgcga ctgacaaatg aatcgcttta cgtgttgcgc aagtttgcga ccgagttggt
    14221 cggcactgaa aactacacga cgactgatct gttcagcctt aaaaactttt tcaataatct
    14281 cggcgcgcaa ctcgcaactc atcgcgacat tagatacgca aagacaatcg tgcagatcgg
    14341 cggcgatccg agtgagttgc agccattgac tggcaagcag atgcgacagg cagtacgtaa
    14401 cggtggcgcg acgctcgtgc tgatcaattc tgttccgatt cgtttgcgcg aacaggcgca
    14461 ggtgtttctg cacgttagac cggggacaga agatgcgatc gtcctcgcgc tcgctggttc
    14521 gtcaaacgat tcgctcgccg cgcagaaagc cggtgttgaa gcaagtgcga tcgatgcagc
    14581 acgccaggcg attgcaaaca ctgaaggcga cgtcgtgatc atgtttggtc acgaagtcag
    14641 cgaagctgca cagggagtaa tcgctcaact atcacaaaca ttcggcggag ataagcggcg
    14701 aatactgctg cacccccttc cgttcttcaa taacagcgtc ggggcgcacg ccgtgggcat
    14761 gatggaacgt ccgacatcgc cccgtcagat tctcgatgcc gcaggcggtg agattcgcgc
    14821 actctacatg gctggtagct tcctgccgga acatctcaaa ggacgcgatg ccgcgttgtc
    14881 gcaactcgat ttccttgtgg tccaggagct cttcgaaaac gagacgaccg cagcggctga
    14941 cgttgttttg cccgctgctt cgtacgccga acaggacggc acgttcacaa acaacgacgg
    15001 cttcgtgcag cgcgtgcggc agtcgattcc gccgctgcat cagtcgaagc ctgactggat
    15061 gatcgtggcg caactcgcga aagagctggg attggatttt ggctacgaga tgtcagcgag
    15121 cgcagtgttt cgtgagatcg gcgagcacgt gcccgcattc tccggcatgc gctatccgct
    15181 gctgaaggac gagaagaatc cgatacaggc gaagtttgaa gtcggttcga gcgacgtgag
    15241 cgacgctctg acttccattc gagatcacgt tgaatcgtta ccggatgagg gggacaagat
    15301 aacaatgacg cctgatgtgg gaacggagct gttccgtttg ggagggctga cggagaaagt
    15361 gggccaattc cagttgttgg cttccggtaa tccaagaccc gagaccaccg cggtttcgcc
    15421 cttgtatcag atccaatccg tgttatccgt ggctaaataa tttcatggac actgcgatcg
    15481 atatagtcct caagctcgta gccatcaccg tggcgttcac ggtcgtgctt ctgatcgtcg
    15541 cctacacggt gctggccgag cgtcgcgtcc tcggtttgat ccaggggcgg cttggtccaa
    15601 accgcgtcgg ctacggcggc gtgctgcaac cattcgccga tctactgaag ttcattctta
    15661 aagaagacat cgtccccgac aaatcgacac gcttcatcta cttcctcgcg cccgtgatcg
    15721 ccacgatggc cgcgttgatg acgatgatcg tctacccgtt cggtcccgag atcgcgattc
    15781 cgtttacgaa cattacgatc ccgctgacga tcgcgcgttt cgatatcggt ttgctttacg
    15841 tgctcggcat cacttcgatc ggcgtatacg ggatcatgct tgcgggctgg tcttcaaaca
    15901 acaagtacag cttgatgggc ggtctgcgct cgtcagcgca gatgatctcc tacgagctcg
    15961 cgctgggcat gagtctgatc ggcgtcatca tgatggcggg caccctcgat ctcggacaga
    16021 tcgttgagaa ccagtccggt tggcacggca ctcattggta cattgtgttt caaccgctcg
    16081 gttttattat ttatctaatc gccgcgattg cagagacgaa tcgcgttccc ttcgatcttc
    16141 ccgaagctga aaccgagctc gttgccggtt ttcacacgga atacagcgcg atgaagtttg
    16201 cgctcttctt cctcgctgaa tacatcaaca tgttcacggt ttcgatgctg gccaccaccc
    16261 tcttttttgg cggctggaac tttcccttcc tggacctggc tcttacacgt ggcacgttcc
    16321 tgttcggtct cgtgagcgcg ctcggattta tttttaagat ttgtgtgttc ctgtttattt
    16381 acatctggct gcgcgggacg ttaccgcgct ttcgtttcga tcagttaatg aattttggct
    16441 ggaagtttct gttgccgatg gcactgctca acatcttcct gacagccaca ctgatctact
    16501 tctttccgaa cttgtaacta tgcatcgagc ctgcgaagca ggcggaagca taaagcctgg
    16561 ggcgcaagcc ccaggatcat tgaaagaaag attgattgag cccgtgaaac gggcgacagc
    16621 tatgacattc gcaggctgtc gcccgcattc gcgggcttgg gaaatggaaa tgcgaaatac
    16681 ctggggctta cgccccaggc tttatgctta cgcctgcttc gcaggctgac taaatcatgc
    16741 tggcgcttct tttcatcctg ttcgcaggac tggcgatcgg ctgcggtctc gcggtcgtcg
    16801 cgcaacgcaa tccgctctac agcgcgatct cgctgatcgg tgtgttcatc tcgctcgcgt
    16861 gtctctatgt gatgctggcg gcgccgttca ttgcggcggt ccaggtgatc gtttatgcgg
    16921 gcgcgatcat ggtgctcgtc gtgtttgtga tcatgctgct caatgtcgag caggaggagc
    16981 gtggacgccc gcgtttgaag ttcctcgtgc cggtggctgt cgcgctttcc gcaattctga
    17041 ttgctgaagt tgctttcata ctcgtcagcg tgcaggaatt cagagtgaat ccgacgtcgg
    17101 tgccttcgga tgtcggattg acgcactcga tcggcgcgca gttgttcaca cgatatcttt
    17161 taccgtttga gatcacttcg atactgctgc tgatggcgat cgtcggcgcc atgacgctcg
    17221 cgcgtcgcgg tggattgttg ccgccgaacg cgcgcatcgt tccgggggct caagtgcttc
    17281 cgctatcgat ggtcgatcca aacgctgagc cggacgactt gaggccggtt gaagcagaca
    17341 gctcagacga agcagcgcgg gttttaggaa ttactgactt tcaaaagcga gactaagacg
    17401 atggagccac agctttcctg gtacctggcg ctctccgcga tcctgttcac gattggcgtg
    17461 acgggcgtga tcatcaagcg caacgtgatc gcgatgttca tgtgcatcga gttgatgttg
    17521 aatgcggtga atctgacgtt tgtggcgttc gccagtttct ttcaggacgt gacggggcag
    17581 cttttcgtgt tcattgtcat gacggtggcg gcggcggaag cggcggtggg tttggggatc
    17641 atcattgcga tctttcgtaa tcgtgagtcg ctggatgtgg atgaggcgag cattttgaaa
    17701 ctgtagacgc ggatgaacgc ggatttgaag acgcggatct tttcttaggg atggagaata
    17761 atttactcag tttgatcatc tttgctccgc tggcgggagc ggcgatcaat tggtttatcg
    17821 ggcggcgtgt gcgcgatgag cggttcatcg gcatcgtggc gtgtgggtcg gtggcgatct
    17881 cggcgatcgt ggcgttttat ctcgcgctca attcaggggg ggcgttgcgc actgaaacgc
    17941 cggtgctcga tcatatctgg acgtggttgc aggtcggcaa tttccatgcg gacttcgcgc
    18001 tcggaatgga tcgactgagc gggacgtacg cgctcttcat cacgttcgtc ggttttctca
    18061 ttcacgtctt cgccgtcggt tacatgcacg gtgaagcggg cttctatcgc ttcttcgcat
    18121 ttctcaacct gttcatgttc gcgatgctga cgctcgtgct cgccgacaac ttcgtgctga
    18181 tgtttgtcgg ttgggaaggc gtgggcctct gctcgtacct gctcatcggt tattacttcg
    18241 acaagaagga agcgggcgac gccgcgaaga aagcattcat cacgaaccgc atcggcgact
    18301 ggggcttcgt gctcggcatc atgctgacgt tcttcgtcac cggctcggtc agtttcttcg
    18361 ctaatccagc cgctggtgaa gccgcgcaca cggcgttgtc gttcttcaga aaccaaccag
    18421 tcgagacatt cactgccggg gcgatcttcg cgggcggcgc cacgtcgatc gcggtgctgc
    18481 tcttcatcgg cgccgcaggc aagagcgcgc agattccttt atacgtctgg ctgcccgacg
    18541 cgatggccgg cccgacgcca gtgtcggcgt tgattcacgc ggcgacgatg gtcaccgcgg
    18601 gcgtctacat gatcgtgcgg tgctcggaga tcttcgtaca cgcaccgaca gcgatgttca
    18661 tcgtcgcgat cataggcgct gccaccgcgc tcttcgcggc tacgattggt ctggctcaaa
    18721 acgacatcaa aaaggtgctc gcttactcga cgatctcgca gcttggatac atgttcctcg
    18781 cgtgtggcgt cggcgcgttt attcccgcga ttttccacgt catcacacac gcattcttca
    18841 aagcgctgct cttcctcggc tcaggctcag tgattcacgg catgcatcac gagcaggaca
    18901 tgcgacgcat gggcgggctg aagaagtaca tgccgatcac gtttgttacg atgatcacgg
    18961 gcgggctggc gatcagtggt gtgcccttct ttgcgggctt cttctcgaag gacgagattc
    19021 tctggcggac atggagcgcg cccgcgttta gtcttccctc gaccgggttc agcaagtttc
    19081 tctggttcat cggtgcgctc acggcgtttc tgaccgcgat ctacatgacg cgcatgatgg
    19141 tgatgacctt ctggggcaag gagcgcttcc gcgagacgca cgattcaaaa catcaggagc
    19201 cggatcatca tcatggaaca gttgagccgc acgagtcgcc ctggttgatg acggtgccgc
    19261 tgatcgtgct ggcagtgctt tcgactgtcg gtggtttcat cggtattcct tatgcgtttg
    19321 tgggtaagga cgtcaacgtg atcgagcgca cgctcgaacc ggtggtgccg aaagctggcg
    19381 cgcctgttgg gacgcatgcg accgaagcgc cagttgtcga gcacggcgaa gcggctgcca
    19441 ctgagaccgc gcacgcaaca cattcgagcg aagagttaaa tgcggagcgc atgctcgccg
    19501 ggttatcagt gctcattgcg ttgttaggca tcggcgcggg ttggtacatg ttccagaaac
    19561 gaccgctgtt gcagatgccg cgcttgctgg aaaacaagta ctacgttgac gagttttatg
    19621 acgccgcgct cattcacccg attgaagtag cgtcacgcga aggtctctgg aaaatattcg
    19681 acatgggagc gctcgatggg ggcctgcaca cgattggtga agcggttcag gaaatgggac
    19741 ggctcgctcg tcacctgcaa gccggtttcg tgcgtggtta cgcggcgata attctggttg
    19801 gcgcgctgat cttgattgga gtgtttgcat acctcggatt tccgggtgtg taaccacaga
    19861 ttaaacggat tgtttacgga ttgatgtcct atttgttgac catactgatt ctgttaccgg
    19921 tggcgggaac gctggcgctc gtcggctata gctttgtgtc aagcaggcgc gaggagcatt
    19981 accgctggat cgcgctgatc gtgaccgcag cgacgtttgt cttatcgctg ctgttgctcc
    20041 gcggtatcgg tgcgagtggc gctgaattgc ggttcgaaga gaacgtgagc tggatcggcg
    20101 cgatcggctc gcggtatcac gtcgcggttg atggtattag cttatggctt gttttgttga
    20161 cgacgctgct gatgccgatc gcggtgatat cgagctggac cgccattcac aagcgacaac
    20221 tgtcttacta cgcgttcctg ctgatcctcg aatcagcgat gatcggcgtc ttcgtgtcgt
    20281 tcgatctact gctcttctat ctcttcttcg aagcctcgct cgtgccgatg ttcttcctga
    20341 tcggaatctg gggtggcgag cgtcgcgttt acgccgcggt taagtttttc atctacacgg
    20401 cggtcggtag cctgctgatg ctggtcggca tcatctcgct ctatttcctg tatcacacgt
    20461 ttgattatcc aacgctctta caggcaataa cagcaacacc gctggccaat tttggccgcg
    20521 ccgaatactg gctgtttgtc gcatttgctc ttgcgttctg tattaaggta cctctcttcc
    20581 catttcacac gtggctgccc gacgcgcaca ccgaagcgcc tacggccggc tcggtcattc
    20641 tcgccggcgt gctgctgaag atgggaacgt acggcctgct ccgctttaac ctggcgcttt
    20701 ttccgaatgc atcacgtgat tgggcgtggg tgatcataat actcgccgtg atcggaatca
    20761 tctacggcgc gctcgtggcg atggtccagc ctgacgtcaa acggctcgtg gcgtattcgt
    20821 cggtgagtca catgggcttc gtcgtgctcg ggttgttctc gtttacagag ctcggcatgc
    20881 agggcgcgct ttaccagatg ctcaatcacg gcgtctcgac cggcgcgctg tttctcttcg
    20941 tcggcttcat ttacgagcgg cgccacacgc gccagatcag cgagtttggc gggctggcga
    21001 aaccgatgcc gtggttcgcg acgctgtttg tgtttgcgtc gttgtcgtcg atcgggttgc
    21061 cgtttttgaa tgggttcgtg ggcgagtttt tcatactgct ggggacgtgg acgtcgcgag
    21121 cagtcggtcg cccgtggcta accacgatgc tcgcggcgac gggcgtgatc tgggcggcgg
    21181 tttacatgct ctggatgtta caacgcgtgg tgtttgggcc gattacgaat ccggagaatg
    21241 ccgagttgtc ggatcttaat aagcgcgaga tcggattact gataccactg ctggtgctga
    21301 tgcttttcat gggtgtttac ccgcgcgtgt ttttggatcg ttcgaagcca agcgtcgaag
    21361 tgattcgtgc ccgcgtctcg tcgagcccgg ccggaggttc gtacacaacg gaagttgtta
    21421 gacaggatta acaggatttt gcaggattat catgtaaatc ctgtcatcct gtaaaaaggt
    21481 ttgatgagct catttcttgc acaatccgta atgcccatcg tcaactggtc gttaatcgca
    21541 cccgaggtga ttgtttgcgc ggctgccgtt ttagtaatgc tggttgacgc gtttgtgcgt
    21601 ccgacccagc gctggatcac cggcggcatt tcactcgccg gcatcatcgc ggccgcgatt
    21661 agtaccttat ggctctggtc cacgggcacg ccttcgtccg atgcgttcaa tggcatgatc
    21721 gcgctggacg agctacgcct cggcttcact ttgatcttcc tgctcgtttc ggccctcacg
    21781 ttgctcatct cgactgtctg ggttcagggc gaacaactgc ccgcgggcga gtttcactca
    21841 ctactgttat tcgccactgt cggcatgatg ctgatggcct cgggtaatga tctcgtcatc
    21901 atctttctcg ggctcgagat actctcgatc gcaacttacg tcatggccgg cttccgtcgc
    21961 actgacgtgc gctcgaacga atcatcgctg aaatatttca tcctcggctc attctcatcg
    22021 gcttttctgc tctacggtat cgcgctcatc tacggcgcca cgtccattgc cgaacccggc
    22081 ccgggcggtt cgctcagccg tattgtcgcc ggcacaacca acattgccga gatcgcccag
    22141 cgcgtcggcc aggcacagta tccggcactg ctcttcgcgg gcgccgcgat gatgctcgtc
    22201 ggttttggtt tcaagatcgc gactgcgcct tttcatatct ggacgcccga tgtttacgaa
    22261 ggcgcgccga cgcccgttac cgcgttcatg gcggcgggtc cgaaagcagc aggttttgct
    22321 tcgttcatgc gcgtgtttat cttcggactg ccgttcgtag tttcgacgtc gaatgcaacg
    22381 accggaaacc tgcatcacgc gtgggtcagc gtgctcgtcg ttatggcgat actcacgatg
    22441 acgctcggca acgtcgtggc gatcgtgcag aacaacgtca agcgcatgct ggcttactcg
    22501 tcgatcgcgc atgcgggata tgcgatggtc ggttttatcg cggccggctc cgctaccgat
    22561 ccggtacagc gccacaatgc aatcacctcg gtcgtgttct acctgttgac ttacgcggtg
    22621 atgaacatcg gcgcgttcgc ggtcgtgcaa ctaatcgcgc gcaatggcga tcgtcgcacg
    22681 tcgattgaag actatcgcgg aatcggattc gaatcgcccg tgcttgcgtt ttcgttgagt
    22741 ctcttcattt tgagtttgtt cggcatgccg ttgactgccg gattcatggg caagattctc
    22801 gtgtttggcg cggccatcga tcagaagttc tacggcctcg tggtcgtcgg cgtgctcaac
    22861 accgccgtct ccgcttatta ctatctgcga ctaatcatcg tgatgttctt tggtgaacga
    22921 acgatggcgt ggagcgcgcc gcgcgtgccg gcgagtgtgg cggtggcgct cgtgatcacc
    22981 gtgcttggcg tgctttacct cgggattttt cccggccgcg tgatcaatgc gctgcaaacg
    23041 cggatcgagt cgcagttgtt tacgaaccaa ttacacagat agtgccggcc gaaagttacg
    23101 cccaactgat tcaggaacaa cttggggaat catggttgcc gcgaatctat cgcgagcgca
    23161 tcctcaagct acgcacacgc tcgtatcact tcgaaagcgc gaagcctgcg gcgcgtgtcg
    23221 agatccagca cacgctgctc ggtgtcgaac tgaaggtcgg gcggcggcgg ctcctgtgtc
    23281 cggatctggc cacggcgaga tacctctcag tcttcgcgcg gcttggttgt gaggatgtag
    23341 cggttccgta cgacataaca aaaatctcgc atttagcgga cgaattggag tcgtcgtggt
    23401 atagaatgtt tttactagtt gagcaggaaa ctcgtgcgaa gagcacgcga tttagtgcga
    23461 gactccgtgg cctgctaatt gctaaaatcc gcgaggagat caaggaggct ggaccgggga
    23521 cgcgtgttcc ggagttcagg caaacgacaa aacaacgtac gagatagttt tacaaatttt
    23581 agattaaagc cttttttaac gacaatcccg aatgactcaa gaatacaccc ccactacttc
    23641 gcttcccttt ccatttaacc agcagaactt ctgtcgctac gagcccatca accagagcat
    23701 cgacgaggcg cgtgtgctcg taatcgacaa ccttttgcgc gacgaacgcg acctgtcttc
    23761 agtagcgcgc gcggaatggg gcgagacact cgccgccgaa atgcgacgcg agcgactcat
    23821 cgccggcatg gcagttgaaa atattctcaa caacatttct cgtctggtta agacaccgac
    23881 aaccgaagtc gcgcaccttt cggaagtcgg cacggccgcg aagagttttg cgcccgacgc
    23941 gatcgtgctc agcggcacgt tgcgcgattt tgattactac gatcaatcga tactcgatga
    24001 cttcggtcaa tttatccgca acacgaaaac gcccgtgctc gcgatctgcg gcggtcatca
    24061 gctcgtcgga ctgagtttcg gtgcacgcgt gatcacgctc gacaagctcg agcaacacga
    24121 gcagcgcctt gcgcgtccgc aggagtacca gtatcgcttc atccgcatca ccgagcccga
    24181 cgatccgatt ttcaaaaaca tcaactgctc ggaaccggat gcgcctgcgc gaatcctgcg
    24241 cgtctggcag aatcacgggc tgcaactcga tcgcattccg gaaggattcg agctgctcgc
    24301 gacgtcgtat ctctgtcgca atcagatgat ggttaagcgc agtgacgggc agatgatcta
    24361 cacggtccag tttcatctcg aaaaatcgtt cgaagactgg aacaagagcc gcacgcgctg
    24421 gcagcatccg aatgattcgc gcgatggtcg gatcctgttc gagaatttcc tgacactcgc
    24481 cgtccagaat tgaggccgct cacagaaaag actctcgctg tcgcggcgcg tgagctcgcg
    24541 acacgcgacg agatgttggc tggaatacat gccattcacg gcgatccgcc gctctggcgc
    24601 cgcgccaccg gattcacaac gttagttcac atcatcctcg agcaacaggt gtcgttgcag
    24661 tcagcgaagg cgatgcttgt gcgtttgcag ggaataattg aaccgttcac accggagcgg
    24721 tttgtggagc tgggctatga ttacctgcgc agtcttggtg tgacgcgaca gaagtcagcg
    24781 tacctgatcg atctctcaac ctcgatcgtc aacggtcagt taagcttcac aaaactcgcg
    24841 cgcatgagtg atgacgacgc gcgactggcg ttgacgcgga tcaaaggtat cggattgtgg
    24901 agtgcagatg tttatctgtt gatggcgatg cgccgcgcgg acatctggcc ggcgggtgat
    24961 cttgcattgg cagtagcgat gaaggagttg aaaggattgc ccgtgaggcc cagctcgctc
    25021 gaactggaac aactcgctga acagtggcga ccgctgcggg cagtcgctgc acgaatgctc
    25081 tggcagtact atctcgctaa aagaggtaca caaaaaagca caaaagaaca aaaggcacaa
    25141 aaagaaagtt cagagttcaa actttgaatt tcttctttgt gccttttgtg gtcttctttg
    25201 tgctttttgt ggccctattt ggctatcgtt gaagcggtcg tagtggtttg cggcggtatg
    25261 gttaaatccg gtgtatccgg cacgttctcc atcgtcaact taatcagatc atcaaccacc
    25321 gccttgagat cgttcgtctc atgatagacc tggagctgcc tgtctgcgga agtaccgcgc
    25381 tcgaggatcg tgtgaatgtg ctcgatctct ttccgcgatc cgagctcatc caccacctca
    25441 tcgacaaact ccagtaactc catcaccaga tcgcgcacgg gcacttccgt ctgttttcct
    25501 aaatcgagca acttcccgtc caacccataa cgcaccgcgc gccacttgtt ctcctgaatc
    25561 agcatgcgcc ggtataaccg gaatccaaga ttctggtcga tcaacttatt caacttcgcc
    25621 acgatcgcct ggaacaacgc cgcaatcgca atcgtgtcgt caacgcgcgt cgggatatca
    25681 caaacacgaa actcgagcgt cggaaagtat ggatgcggac gaatgtccca ccatatcttc
    25741 tttccatcgt tgatgcagcc agttttaacc agcagatcga cgtagcgctg gaaaccaagg
    25801 tacgaatcga agtgatcggg aatgtctgtg cgcggaaact gtttgaagat ttctgtgcga
    25861 tacgacttga gcccggtgtt gtgacccatc cagaacggtg acgacgtcga gagcgcaagc
    25921 acgtgcggca ggaaataacg cgcggcgttc atgatgtgaa tcgcgcgctc gcgatccgcc
    25981 atcccgacgt gtacgtgcaa tccgaagatc aacaacgacc gcgccacagt ctgaagctcc
    26041 tgcaccagca gctcgtaacg ctcgtcgtcg aagatcttct ggtcctgcca gcgcgagatc
    26101 gggtgcgtcg aagcagcgac gatcacgagg ccattcttcc gcgccagccc ggcaatgatg
    26161 ctgcgtagtc gcgtgatgtc ggtgcgtgct tcctggatgt tgcgacaaat gcccgtgccg
    26221 acctcgacca tcgactggat catctcgggc ttgatctgct cgccgagcaa catcttgcct
    26281 tcttcgagaa tctccgccac gtgggagcgc aactcgcgcg tctgcggatc gacgatctga
    26341 aactcttctt cgatacccag tgtgaattga tcgaacattt gccctctttc tactctgcgg
    26401 ttctctgcgg ccctctgcgt acctcagcgt cctctgcgtt tacgtgcgct tctaacgcag
    26461 agggccgcag agatacgcag agaccgcaga gacggtcact tatccagcca cttcgcgatc
    26521 attttccagt caggcttcac cttgcccatg aactcgtgtc gcaagtgctt ccacatgctg
    26581 cccggcgagt gaacgggact gtaaagatcg aagtgaacat accacgtgcg cggctcacct
    26641 tcctgaaaca aaaaatgcag caccgccgcg gcccttcgct tttcgtaaaa accaccggtg
    26701 gtgtctttgt ggttcgccat cagccgcgtg aaatcattac gacgcgagag tgtcgattcg
    26761 atcatcggcc acgcttcaaa ctgtaacccg acaccgcctt caccatatac attagttatt
    26821 cgtttgacga ggtgccagag ctttttcgtg tccattcgct tcaggagcag cagcagcgcg
    26881 tgtttcttgt cgagcggcca cgagtcgaaa gcggcggcat cgctctctgc gccccacagg
    26941 ccagcgagtt ttccgctcag catctccctt gtttcgccgg aaagatcagc ccacgtcagc
    27001 tcaacttcag ccattggaaa cttcgcgctc gagcatcgcc cgcacgtcgt ctgacaaccc
    27061 ctggaacctc aagccaaccc cacgaccggg attgctgtaa accaccactg cattgcacac
    27121 cgcctgcacg tcgtgccgga aaggtatgcg cacgagcaac agcgtgcctt ccggaatctc
    27181 cgcctgcgtg ttcatgtaaa gcccgcccac gctgatgtct ttcgtgctgg cgactccggt
    27241 aaggtcttta ccgtcaaaga aaacgtcgac gatcagccgc gagcgattgt agcttctgcg
    27301 ctcttcctgt gtttcaaagc ctcggtcttt atcgtcaggc acgcgagcct cctgagtaag
    27361 gtcctgaagt cgcctcgatt gtactcgttt tcctcacttt ttgctgatga tgtaggtggg
    27421 caccatgccg ccgccggagc tcacattcgc cagctcggtg aatgaaagcc gcgtgaacgc
    27481 cgagccgact ttgccgttga aaagcaatgg gtcgaggtcg acgagctgtt cggcgaattc
    27541 gatgcgaccg tcgtccgtga gcgctggaaa agtctcgcgg gcggttggca cacgttcctg
    27601 cacgagataa tcgccgtcgg cgagcgcgtt attgatggcc tcgtcccagc cgcgctggtc
    27661 gacgttccag ccaatcgtga tcccgtggcc gccgtaatcg tcgtttggct tgagcaccag
    27721 tcgctcgccg tcagcgcgaa taaagtcgag gaggtttacc tcgcgaccga aataatcggt
    27781 gtgttcgtct ctgacgacgc gcgtccacgg cacgtgcgcg tggatcgcgt cgagctcttg
    27841 ctgcgagaac aacggcgcgt gtttcggatt cgttaggacg gcaaacagcg ctttcttgtg
    27901 gatcagtttt gaacggaagc tgttgaccat gcagatcgcg cccgcgcgat aagcgtcgag
    27961 caaagccgga tattccttga tgattggcag gtattcgttg acgagcaggc gcttatagac
    28021 gatgtcgatc tcctgcccgc gtgcgcttag tcgatcgttg ctgaactcga gttcctgcgg
    28081 cgtgcagatg atggacgggt aacctgactg ttcgaagaat tcgcggaaga gctcgaattc
    28141 tttgacggtc ggcacgtcag ctaaatcgac gatcgcgata cgcggtgggc gttcgggttt
    28201 tctgccgaga aactcttcgt aagagctgag caacacgtcc agcatcaacc gccggccgta
    28261 cagcggacgc acattgtagt cgacggcaaa ttcgcgcatc acgggcagtg acgagaagat
    28321 gtcgtacgcg gcgtcggcgt aagcaatgcc cgccgggctc tcaccgttca gctcgacaaa
    28381 gctgtaggcc gactttgtca ggaacgaatc gagacgcgaa gtgggcgaga ctgcgacgta
    28441 gccgggatcg atggcaacca gctcgcgttc gatctccgtc aggccgagat cgtcgacgac
    28501 attcggatcg gcaacggcgg cgtctttcac cttctgaatc gctgaccaga cggtctcgca
    28561 gatctggcag atgtgtgcga agtctgattc ggagacgaag tgtggacgga gataaggcga
    28621 caggcgacgg ccgccaaaga tgagcctggc cttctcgagg ccctcgtcga ggagggcacg
    28681 agacgattca gccagctcct ggttggaaag caggtcgtga taacgcgcaa cggcttctgg
    28741 gaacatgggc cgttaggtta tcacttgcca caaaaaggca caaaaagcac ataagaagaa
    28801 atcctatttt gtgccttttt gtgactcgat tgattggatc tctttgacga gcgcagcgcg
    28861 gaactgtgtg aggatttcga tgagcaggtc gatctttgcg aggctgatgc gccggtgccg
    28921 tttcgttccc aggaagaatt caagctggac cactcgcttg cagtcggcga tgagcacggt
    28981 attgtcgccc cacttatcgc ccctttggac gtaggcctga atgtacgagg tcagatgagt
    29041 tgacgccggg tttaagaacg cgcgttttga aaaggcatag atgattttgc gtgtcatggg
    29101 cgctcgcgtt gtatccggcg gctaacccgt tttgcaagcg cttcgctgat gacgagaagc
    29161 acaaaccgca tgcaaatttt cttacgtgga aagtagacac acgaatactg tcccacaaag
    29221 cgcaggtatt cgatcctcaa tgcaaaaact cggataaggc ccttagcgaa tcaaactgag
    29281 aatcgtaagg attatcgtcg tcgtagtgct ggttgaggtt gatgtttggg tcggttgcgg
    29341 tgctggtgcg cctggcgagt ctatcgtccc gataacgccc ggcgagtcta gctgaccgcc
    29401 aaatgcagag acagccagga ccgcagtaac aatcgcaaca gcgcacattc ggcgtaattt
    29461 attcactatt ttcgtgacct cctcaaattc actattttgt gactttagga agatacaaaa
    29521 attcttgcat caaacccaac ccttcttgca ggcgcagaga aattgcgtta gagtgtacgc
    29581 gcagttgaga gattgatttc ttgcggttct cacgatgacg ttacgggcct cactcttacg
    29641 cgaattagta aattcaaacc tcgatgtggg aggccgtgcg gagctttgtt gcaaactagc
    29701 ccgggagttt gagaacaagg gtgagtatga agaagcgcgc gaaatgctct atggcctatg
    29761 gcctcggatc gatcagaggc caagagtaaa agggctggaa ccgggcattg cggccgaagt
    29821 gttgatgcga gcgggagtgt tgacgggttg gatcggcagt gaacaggagc aagcgaaaga
    29881 cttcatcagc gaaagcctca caatcttcga attccgaaaa tataaaaaga aaacagccga
    29941 agcacagaca gaactggctc tgtgttacat gcggcttggt gagtacgaca acgcatccga
    30001 tcttcttaag ctcgctctca gggagttgat tacagacagc gagttaaagg caaaggcctt
    30061 acttcgcctc ggtattgtta agcgacacgc aagcccactt aatgaagcgt tgacgtttct
    30121 tacgaacaat gaatctctat ttcataagat caataatcaa actcttaaag ggtgttatca
    30181 ccagactatc ggtgacgtac tcgagaatct ttgtgaatcg gtagggccga aagactatct
    30241 tgatcgcgct ttaatcgagt acgctgccgc gagccatcac tttgaactcg cacaacacag
    30301 atactatctc gccaatgtcg aaaataatct cggcttcttg ctttataagg ttgaccaatt
    30361 tgaagaagcg catgagcatt tgaaccgcgc cagacgcatt ttcacctgtc ttaaggatag
    30421 gattgcgcta gccgtggttg acgaaacgcg cgctcgcgtg tttcttaaag agaaacggaa
    30481 tgcagaagca gagaaagccg cccgctcatc agtgcagacc ttagaatctc gtgatagacc
    30541 atcattgtta gcagaagcgt tgaaacggca cggcatggct ttagcccggt tgggccacta
    30601 cagcgccgcg ctcaatgcct ttcgacgtgc tatcgattta tctcaggata acttcaaccg
    30661 cgccgcagac attgcgctca cggcgtttca ggaattggga gatcgattag ccgttaaaga
    30721 cgcgataact acttctggca gaccattgaa cgaggaaata ttctcactcg agcattcgtt
    30781 aataaaacac gcgctcgaga acgcccacgg ccgcgtcacc ttcgccgccc gcaccctcgg
    30841 catgtcatat caacgcctga ctcacaaact acagaccaaa cacaaagacc tactcaaaga
    30901 ccgtagcccg ctgcgtcacc gtacccgaaa accgtaaact gatggtaaaa ctcggctttg
    30961 tgtgctacca caagtcgtcc gctttttttg tgagggtgaa acattataaa taaggtggat
    31021 ctctcaccgt accattgtga agaagaatct tgagacacaa acaccgggag aattggaata
    31081 tgtctgaaga tatcgagatt gaagacgcta gtggccgcct cggttcaatc acatttgaca
    31141 ggagtccgcg aactcttacc gcagagcgca ccgcctcagg ttttgaactg caattaccaa
    31201 ttgagattac gctgcgggtc cgcttgcgcg atgatccgat gctgatgctg agcaatctgc
    31261 ggggtagcgt gctggtaaaa aagcccgatg gttccccgat aaatgttggc acactacttg
    31321 gcaaattccg ttacactgcg ggtatttcta cctctgaacc ttacagttat aagaacacca
    31381 agtatttgtc atggacggga acatttgcgg atctggcaca tatcgaacag tgtcgtaatg
    31441 gccagcctcc tcagttccta atgcacgttg aaggtgaatg gtctttctta gtgccacccg
    31501 ttctaccatc agcatctgag attccccagg agttgctatc tcaagagctt gaaactcggc
    31561 tgcgcaaata tcagtcgttt agatttttga ctgaaccaca ggatgtttat agcaggaccg
    31621 gaaatattca agttacatac ccacgagaat tatgggtgga aatgctcagg aggttgggtg
    31681 tggccgaaaa tgttcttgtt gaggtgcctc tgccagcaaa ccctagtggt ccatgggacg
    31741 atgtttggca agcattgata gacgctcgca atgcattcga acaaggcggc tcgaccggtt
    31801 ggcagtcgac tgtcacttcg gtgcgccgcg cgctcgaacg atggcacgaa attgaacaag
    31861 aggatcaagg tccagcctgg aagactccta gccagtcgga cctgaaatta cgaacaaaga
    31921 aacagcgtct cgatgcactg cgctggcatt tacggcaagt cgcccatctt gcgccacata
    31981 gtagtgctga agaatggacg agagatgatg cgttattgat gctggctacg ctttcctctt
    32041 tgttagcgga gaggaagccg tagagtttcg gccggctcac tttgtgcctt tttgtggcct
    32101 acttcttttt cgcgggaacc ttgacgacgc gtttttgttt cttgcggacg acctcgaaag
    32161 tgtacgaatc agaattcgga gtcgtgcgga atgcggaaac gggtttgccg ttgattgtct
    32221 gaatgacgtc acccggctcc agaccggctt cgaatgccgc agtcaccgga tcgacgtaga
    32281 taaccaaaag ccccggattg gttccgagtt gagaagctac cagcggcttc aaggcgatcg
    32341 tctcgatccc ctgatcgatt aaccaaaaac ctttcatcga ggggaaacca gagcgccagt
    32401 tgactctgga ccactggtct gacatgccgc tcaacttcac attcatggcc cattccatcg
    32461 gacgatcggg gcgcgtgact gtaaactcca ccgagctact cggcccagcc tgatcgagcc
    32521 accaggtaaa ctcatcggca gtctcaatat ccttgtcgtc gaccttcaaa atcacatcac
    32581 cggcgcgcaa ctgcgcacgc tcggcgggac tgcccggcac gatcgacgtc aacagaattc
    32641 cacgatgttt gccagccaac gccgccgcgc gagtcatctc ccaaccgtga ttctggatct
    32701 gatcgagttt gagcgctgcg actgcttcac ctttcacccc cagccacggc cgcggcacgc
    32761 ttgcctgccg ttccagcaca cgctgggccg cgcgtttgat tgacgctgcc ggcagaatgc
    32821 tcgcctcgtt cccttccaaa ccgtcgataa tgccaatcgt gtcgccggcc tgattgatag
    32881 caacgccgcc gatgtttgac tttgaaagtt tcgcggcact gactctgaat cgcgcaattc
    32941 cacccgatgg cgcaggcact acatcttcaa cccgaccttc aatcgcggcc atgcgcgcca
    33001 gcaccgggcg cgttttagtc accggctctg gtccaaacag caacacaacg tcaccactgc
    33061 taacctgttc ggctttgatt gcgcttgccg aaactgcact cttgttttcg agccgcagga
    33121 ttgagagccc ggtagcagcg tcgagtccga tgtatttcgc gggaatctgt ttgccatccg
    33181 gaccgatgac tgacacgtcc ggaccatcaa agaaaccatt gacggcgccc ggaaacatcc
    33241 cggttgcagc acgcggtgat ttcggtggac ccgggcgcac cgggaacgcg accacgggtg
    33301 gtccaaactc gacatcagcc tcaggcaacc aggcggcgat tgtttcgccg tcgtccatcg
    33361 cgagcccggc gataacgttg gtgtgcacgt cgtccatcag gttgaaggcg gagtcaagcg
    33421 catcgatcga ttgcacctgc tcggaacgca gcagcaggcg aaacatcttg aggccattga
    33481 gacgatgaat gatcgttact acttgcggcg cggcgggcgc cttcggggcc tttggagcgg
    33541 gcgctggaga cggcgcaggc gtttgcgcag tcgcgattgc ctgacagagc aatacgctca
    33601 ctaacaacac cgctgaaatt atgcgtctga aattcatcgt cgtaatgctt accaatcacg
    33661 cttcggtgcg aactgattcc cgcctggccc gattcgttgt gaaccaaaac tgacggtcgg
    33721 cacggaaatg gttttcgcat tgccgcgacc atcctcaagg gaaaccgtga acgactggac
    33781 cgacgaatcg atcggaaaag ctgctgacat accggcgggt gttgttccgt taagaactga
    33841 cgcgcctaca ctgctgacat cctttgtcga tagcggttgc cttctgtagt cgatttgagt
    33901 gggccggatc tttctaatcg gcgcagtggt aacacctgca accacctgat ccggtttctt
    33961 atcctcctgg acaactggcg gcgcctggtc agtcttcacg ggttggtgca cttcaggctt
    34021 tcgggccacc tcaccgttat tctcgcccag tctcatagca tctcgcaccg caacaacacc
    34081 catcagaact ataatcgcca atgccgcggt gaccagaccg cctcgcacta atctccagta
    34141 aattgccgga ccgctgctcg catcattagc cagccgcgca cgcagacgga aatcaaaatc
    34201 tgccggcgcc tcaaccgtgc cgagactgcc aacaatctgc cgcaacttcg tctgctgcac
    34261 atgaaactcg cgacactcag cacattcatg caagtgcccg gttgcagcag cgctccacga
    34321 ttcaccaagc atcaactcat cgagctctcg acgaatcact ttgcaattag tttttctcat
    34381 aacttccttc acctacaaat aagcccgcat cttctcgcgc aggaatcctc tcgcccggct
    34441 gatcctcgac ttcaccgttc cctcgctcgt ttccaggatc ttcgcgatct cgtcgtaact
    34501 gcgcccgtcg acgtcgcgaa gaatcagtgg cgcgcgatac ttttcaggaa gtgtgccgat
    34561 agcacgctgg accgcgttgc gtcgctcagt atcgacgagc tcgaggtcct gcaaaggacg
    34621 tccgtcgggc ggattcaact ccttcgcttc gccgccatcg gtttgttgga agaatccagt
    34681 cagcgacacg agtttgcggc gcttacgttt ccgca
//
LOCUS       DASSFQ010000201         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1719630, whole genome shotgun sequence.
ACCESSION   DASSFQ010000201 DASSFQ010000000
VERSION     DASSFQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719630"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFH96_07120"
     CDS             <1..484
                     /locus_tag="VFH96_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973781.1"
                     /translation="FIVSLPLITELLPATESSAVAPGYLQSIAELNSDVLSGLKVLTV
                     DDQQDTRELITLALGRYGAEVRASDSASMALKMIEDWKPDVVVSDIGLPEMDGYDFMR
                     RLRELEGDGERIPAIAVTGYAGALDESKALNAGYALHLSKPIELNELAKAIARVSRRV
                     "
     gene            complement(547..1986)
                     /locus_tag="VFH96_07125"
     CDS             complement(547..1986)
                     /locus_tag="VFH96_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6973782.1"
                     /translation="MNVELQVPSFELATEWLNRRITARQTKGHPTLVHFWSVSSEASK
                     TNLAQVAQLRDQRKREGLRVVAVHVPQSDAEKDPGTVRDAIARLNLTEPCALDNDYKV
                     SHGQENLPAYFLFDPEGEIRASATGANGLDTIEDKLDQLLNELRSKNPFCAACELFLN
                     KEAMFCAECGSPLTLPGSATHPYYESHFVASLPTVQFVNTDPLIGHTIDGKYELVAKL
                     GEGGMSVVYRARRVHIGDDVAVKILTGKFVKDDAALARFRREARAAAMLRHPNVITIH
                     DFGETDDVHAPAYIVMEFVRGTPLRELLRSQDHFSVERGVRLMRGICAGVGSAHRQGV
                     VHRDLKPENILVVAPDDEFELESVRVVDFGLAKLLADAGATSSGAVVGTPFYMSPEQC
                     LGEPLDARSDVYSLGTMFYEMLSGKRPFEAETVSGVINRHLQEPPPPFAEALSIPRRI
                     SVGIMHALAKDPDSRPQTATELARQTGLV"
     gene            complement(2013..2288)
                     /locus_tag="VFH96_07130"
     CDS             complement(2013..2288)
                     /locus_tag="VFH96_07130"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HET6973783.1"
                     /translation="MPKYVIERVVPGASKLTPAELKTLSQRSCSVLNEMGPQIQWVQS
                     YVTDNKLYCVYVAPNEEMIQEHARRGGFPANHVAQVKTIIDPTTSEE"
     gene            complement(2309..>2793)
                     /locus_tag="VFH96_07135"
     CDS             complement(2309..>2793)
                     /locus_tag="VFH96_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973784.1"
                     /translation="ELSNDFESARKHYRRAAELKPYDEDAWMFLAGAYRKLGNVTSAD
                     KAAIKVIEITSRKLEASLDDIIVMSRLAEAYARFGSREESTATLRRVLELEPNDGLAV
                     YNCSCAYALLGEKNEALLLLRRAFESGFRTVAHWARADSTFDLIRDDEEFQKLLIELQ
                     "
BASE COUNT          570 a          805 c          772 g          646 t
ORIGIN      
        1 gttcattgtc tcgctgccgc tgattaccga gttgctgcct gctacggaat cgagcgcggt
       61 agcgccgggt tatttgcaat caattgcgga gctgaattct gatgtgttga gtgggctgaa
      121 agttctcact gttgacgatc agcaggacac gcgcgagctg atcacgctcg cactcggtcg
      181 ctacggcgcg gaagtgcggg cgagcgattc tgcgtcgatg gcgttgaaga tgatcgaaga
      241 ctggaaacct gacgttgttg tttctgatat cggcttgccg gagatggatg gatacgactt
      301 catgcggcgg ctgcgtgagc tcgagggtga tggagaacgc attcccgcga tcgcggtgac
      361 aggttacgct ggcgcgcttg atgagtcgaa ggcgctgaac gcgggttacg cgctacatct
      421 atcgaagccg atcgagttga atgagctcgc gaaggcgatc gcacgcgtgt cgcggcgagt
      481 gtagttaaga tgtccgataa actttagttt gtcgcgacct tacgacaagc taaagcttat
      541 cggacatcaa actaaccccg tctgccgcgc cagctcagtc gccgtttgcg gccgcgaatc
      601 cggatctttc gccagcgcat gcatgatgcc aactgaaatc cttcttggga tactgagcgc
      661 ttccgcaaac ggcggcggcg gttcctgcaa gtgcctgtta atcactcctg agacagtctc
      721 ggcttcgaac ggcctcttac ccgaaagcat ctcgtagaac atcgtgccca ggctgtaaac
      781 atctgaacgc gcgtcgagcg gctcacccaa acattgctcg ggcgacatgt aaaacggcgt
      841 gccgacaacc gcgcccgagg acgtcgcgcc cgcgtcagcc aggagcttcg ccaaaccgaa
      901 gtcgaccact ctcacgctct cgagttcaaa ctcgtcatca ggcgcgacga cgagaatatt
      961 ctctggtttg agatcgcgat ggaccacgcc ttgtcgatgc gctgagccga cacccgcgca
     1021 gatcccgcgc atcaatctca ccccgcgctc gaccgaaaaa tgatcttgac tccgcagtag
     1081 ttcacgcagc ggcgttcctc tcacaaactc catcacgatg tacgccggtg cgtgtacgtc
     1141 gtcagtttca ccgaagtcgt gaatcgtgat gacgtttgga tggcgcagca tcgccgcagc
     1201 gcgtgcttct ctccggaaac gtgcgagggc ggcatcgtct tttacgaact tgcccgtgag
     1261 gatcttcacc gctacgtcgt cgccgatgtg tacgcgacgc gcgcgataaa ccacgctcat
     1321 gccgccttcg ccgagcttcg caacgagctc atacttgccg tcgatcgtgt gtccgatgag
     1381 gggatcggta ttgacgaatt gaaccgtggg cagggaagcg acgaagtgac tttcgtaata
     1441 cggatgggtg gcgctgccgg gcagtgttaa cggcgagccg cactctgcgc agaacatcgc
     1501 ttctttgttc aggaacaact cacacgcggc gcaaaatggg tttttcgatc gcagctcatt
     1561 gagcagttgg tccagtttgt cttcaatcgt gtcgaggccg tttgccccag tcgcggaagc
     1621 gcggatctcg ccttcgggat cgaacaggaa gtaggctgga aggttttcct gtccgtgact
     1681 gactttgtag tcgttatcga gcgcgcacgg ctcggtgaga ttgagtcgcg cgatcgcgtc
     1741 gcgcacggtg cctggatctt tttcagcgtc agactgcggc acgtgaacgg cgacgacgcg
     1801 caatccctcg cgtttgcgct gatcgcgtag ctgggccacc tgggccaggt tagtcttcga
     1861 cgcttccgag ctgacagacc agaaatggac cagcgttgga tggccctttg tctgtcgcgc
     1921 agtaatccgc cggttcaacc actccgtcgc caactcaaat gacggcactt gcaattcaac
     1981 gttcatcggc taaaaatatt cttttacgac cattattcct cggaagttgt cgggtcgatg
     2041 atggtcttca cttgcgctac gtgattcgcc ggaaagccac cgcgccgcgc gtgctcctga
     2101 atcatctctt cattcggcgc gacgtaaaca cagtaaagct tgttgtctgt cacgtagctc
     2161 tgcacccatt gtatctgcgg tcccatctca ttcaacacac tgcacgaccg ctgcgacaac
     2221 gttttcagct ccgcgggcgt cagcttgctg gccccgggca ccactcgctc aataacatac
     2281 tttggcattg atgttctcca gtctggattt attgcagttc aattaataac ttctgaaact
     2341 cttcatcatc acgtatcaga tcgaaggtgc tgtccgccct ggcccagtgg gccacagtcc
     2401 tgaatccact ctcaaacgca cgccgaagca gtagcagggc ttcgttcttt tctcccagta
     2461 acgcgtaagc acacgaacaa ttgtatacag cgaggccgtc atttggttcc aattcaagca
     2521 ctcgcctcaa cgtcgcggtg gattcctcac gactgccaaa gcgtgcatac gcttcggcga
     2581 gacgactcat cacgatgatg tcgtcgagac tcgcttcgag ctttcgcgaa gtgatctcga
     2641 tgactttgat tgcggctttg tcagcggagg tcacattgcc aagctttcga taagcacccg
     2701 cgagaaacat ccaggcgtct tcgtcgtacg gcttgagctc cgccgcgcga cggtagtgtt
     2761 tccgtgcact ttcaaaatcg ttcgacagct ccg
//
LOCUS       DASSFQ010000202         7192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_240921, whole genome shotgun sequence.
ACCESSION   DASSFQ010000202 DASSFQ010000000
VERSION     DASSFQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240921"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..886
                     /locus_tag="VFH96_07140"
     CDS             224..886
                     /locus_tag="VFH96_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET6973785.1"
                     /translation="MAGAAFYDLEGTLVSTNLVHTLSFYARRQQGLMASFKKSATTIL
                     SLPVFAVTDQYSRKVFNDLYFQRYKGQSEDRLRYFAQELFEDVIKPAVFPGTYELIDQ
                     SRKLGLRQVVITGALDLSVKPLMEHLGITEYVTNRLEFVNGMATGRLLPPVMAAATKA
                     SWIRTYTEREGISLSDSYAYTDSMSDLPMLSIVGHPAVINPDMRLRQTALHHDWPILN
                     LK"
     gene            937..2535
                     /locus_tag="VFH96_07145"
     CDS             937..2535
                     /locus_tag="VFH96_07145"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET6973786.1"
                     /translation="MALQLRRKTHESVIYIDRDSAPRIMPSGEDFILEDLPIGTRVIY
                     PNQTIKGLPNREAAIRYALNHPLGCDPLFAQLEPGMRVTIAVDDISLPLPPMATPDIR
                     QTILEIVCDMLAGHGVDDVHIIIANSLHRKMTEWEMKRMVGPRIFSDYYPDRYYNHDA
                     EWADGLIKIGETRHKEPLVLNRRCAESDLLIYVNINFVPMDGGHKSVAVGLCDYESLR
                     MHHDPQTIRDSDSYMDPARSALNHKVIKLGKIVDETLNVFHIETAINNRMYGADMDFL
                     LRNEDDFTAFDRMKFEAMRFTMSKLPRAARRKLLHSRPSQYEMTACYAGKTELVHEKI
                     VAKGFEQYAIPIRGQADILITGIPDISPYNVYSILNPLLVQVMALGYHFNFYRNKPLL
                     KKGGVMIVHHPCFDDFDHQFHPSYIEFFNRLLPETRDAFTLREKYEREFANNPSYIEM
                     YRRGNAYHGAHPFFMWYWGENGRQHVGRVIAAGAENAHVPAILGWERADNLTEAIAMA
                     RSYMGNSAEITMLHQPMIGIADME"
     gene            2573..6958
                     /locus_tag="VFH96_07150"
     CDS             2573..6958
                     /locus_tag="VFH96_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6973787.1"
                     /translation="MKLSPTEIYRDRKIFLIGSTGFLGKVTLSMLLHRFPNIGKIYLT
                     VRARSQEESENRFWNNIITAPPFDPLRERYGSALEGFVNDKVIVRGGDIAETNFGYSE
                     EEAQAIADDIDVVINSAGNVTFNPTLESGLRTNVVGTQNVIAFARRMKRPALVHVSTC
                     FVAGNRSGAVWESDSVIGYFPRHREPEMKGVQFSVDQEIADCAKLADRVRDEARDAMM
                     TARFRELARKRLNEEGRDIDDPDALGLAVARERKVWTRTRLTDLGVERAAYWGWPNIY
                     TYTKSLGEQLVAAETEMVRSIVRPSIVESAMSYPFPGWNEGFTTTAPIIFLIRKGQTQ
                     IPADPKLILDITPVDQVASVMLAVSAQACVEEPRLVFQAATGDANANNMERIIGLCGL
                     DKRNYFEEKDSGVKILNKIQGRMEPRPVTVSKFENTSVPMFNAAAKKASSFLDSVRPR
                     WGGGRVGEVIDRVKSSVERVEELTRETTEAFDMFRPFTVENAYVFRSDNVRSLFDRIS
                     EDEQSLLTWNPERFDWYDYWLNIHLPGLTKWVFPTLEDEMRAVPKRVYTYRDLLELFE
                     TSTKRHATRVAMRIERDGRKEQYTYADMRELATRVAGFLANEGIKPGDRVMLVSSNAP
                     EWGMSYFGVLKAGATCIPCDPESSTAEILNFARAGNAAGLICSQAVLDEHPDLQEQLS
                     TRLWTYDDVFAMPDEQTEDERIALLPQRAHAQSVASLIFTSGTTGQPKGVMLSHRNLT
                     SMVSMLSSVFDMTTRDGVLSVLPLHHTFEFSTGFLTPLSRGAQITYLPELTSDALARA
                     IKNGHVTGMVGVPALWELLHRRIKNRLYERSDWIGKAADNLMWVNAWLRDKTPLNFGQ
                     ILFYPIHEGLGGRIRYFISGGSALSEKIQRDFHGLGFTILEGYGLTEASPVLTVTRPE
                     NQMLTGTVGKPLPGVEVRIADPDASGVGEVLARGPNVMLGYYEDELATRKTLVDRWLY
                     TGDLGRVDDDGNLYLVGRSKEIIVDTNGKNVYPDELEEIYGNSVYVKELSIVGLADGI
                     GEKVACLVVPDDEYDISLSRAALHRLVEEHFREVSASLPYFKRVKVLHFTDIELPRTA
                     TRKVKRREVVQIMESLEESQKSGRRGSAQESANADSFWLLGLVSSVANRPRSEVSNDT
                     RLSDLGFDSLMFVELATAIENAGGSISAPERFNEIQDVRELLTVVSRQPSAAPKPETR
                     VDDSKKDDEIHVPSFVKTAGNKAADFLQQFLYDQFLRTHYEGRSNIPPHTNFLVAANH
                     CSHLDMGLTKMALGEFGKDLVALAAADYFFDTKYKRAVMENFTNLVPMERTGSLRQSL
                     RHAKSFLDRGYNALIFPEGTRSMTGIMADFKPVVGYLALHARVGILPMFLDGTYEAMP
                     KGTTLIKSREVGARIGRLLEIEELEEMTKGMPRAEAYRLIAARVRHEVVNLRDRTRYE
                     FDAKELRKLWKSERKKELVTEEVAV"
     gene            6999..>7192
                     /locus_tag="VFH96_07155"
     CDS             6999..>7192
                     /locus_tag="VFH96_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6973788.1"
                     /translation="MPTKKPGKRTARLTTIGTKRRAAIEKRQRAVTLITGGTGFLGSH
                     LVRQLIEEGARDVRVMATSIP"
BASE COUNT         1582 a         1992 c         2113 g         1505 t
ORIGIN      
        1 cgcccaaaac catcgaaaac agtgccgatg tgaccatgac agatcgcaat atttcacggt
       61 gtttcctcag atttatcatc aattaacccc ggattgtcta gcgtcgctat acagtttgta
      121 atacttttgc ttccggttat ctaattccgt ctgaatgcgc tatagtggct tcccacttag
      181 gccgatggcc gcatcgcaaa ctgcaaaaac gccggaggag aacttggcag gggctgcatt
      241 ctacgatctc gagggaactt tggtaagcac aaacctcgtg cataccctct cgttctacgc
      301 gcgtcgccag cagggcctca tggccagctt taaaaagagc gcgaccacta ttctgagcct
      361 tcctgtcttc gcggtgacgg atcagtatag ccgaaaagtc ttcaacgatt tgtatttcca
      421 gcgctacaag gggcaatcag aagaccggct gcggtatttc gcgcaggagt tatttgaaga
      481 cgtgatcaag cccgctgttt ttccgggtac ctacgagcta atcgaccaat cgaggaagct
      541 ggggctgcga caggtagtga tcacgggcgc gctcgatctc tcggtcaagc ccttgatgga
      601 acatctggga attacggagt acgtcacgaa ccggctggag tttgtgaacg gcatggcgac
      661 cgggcgcttg ttgccaccgg tgatggcagc ggcaacgaaa gcgtcctgga ttcgaactta
      721 taccgagcgc gaaggcatca gcctgagcga ctcgtacgct tataccgaca gcatgagcga
      781 tctgccgatg ctgtcgatcg tgggccatcc ggcagtgatt aatccggaca tgcggctgcg
      841 acaaaccgcg ctgcatcacg attggcccat cttaaacctg aaatgaatcc gttttaatcg
      901 gatcttttga tccgtgtaat ccgtggctaa gaactgatgg cacttcaact ccgacgcaag
      961 acacacgaat ccgtaatcta catcgatcgc gacagcgcgc cgcgcatcat gccgtccggc
     1021 gaagatttca tcctcgaaga cttgccgatc ggaacacgtg ttatttaccc gaaccagacg
     1081 atcaaaggtc tgccgaatcg cgaagctgcc attcgttacg cgctcaacca tccgctcggc
     1141 tgcgatccgc tgtttgcaca actcgagccg ggcatgcgcg tcaccattgc tgttgacgat
     1201 atcagtttgc cgttgccgcc gatggcgaca cccgacattc gccagacgat cctcgagatc
     1261 gtgtgcgaca tgctcgctgg tcacggcgtc gacgatgttc acatcatcat cgccaattcc
     1321 ctgcaccgga agatgaccga gtgggagatg aagcgaatgg tcgggccaag gatcttttcc
     1381 gactactatc ccgatcgtta ttacaaccac gatgccgagt gggcggacgg tctcatcaag
     1441 atcggcgaga cacgacacaa agagccgctc gttctgaatc gtcgttgcgc ggaaagcgac
     1501 ctgctgatct acgtcaatat caatttcgtg ccgatggacg gtggacacaa gtcagtggcc
     1561 gtcgggcttt gcgactacga gtcgctgcgc atgcatcacg atccgcagac gattcgcgat
     1621 tcggacagtt acatggatcc ggcgcgctcg gctctgaacc acaaggtcat aaagctcggc
     1681 aagatcgttg acgagactct gaatgttttt cacatcgaga ctgccatcaa caatcgcatg
     1741 tacggcgcgg acatggattt cctgctgcgc aacgaagacg acttcacggc gttcgaccgg
     1801 atgaagtttg aagccatgcg cttcacgatg agcaagttgc cgcgtgcggc gcgccggaag
     1861 ctgctgcact cgcggccgtc tcagtatgaa atgacggcgt gttatgcagg aaagacggag
     1921 ctggtacacg agaagatcgt ggcgaagggt ttcgagcagt atgcgattcc cattcgcggc
     1981 caggctgaca ttctgatcac cggcattccc gatatctcgc cgtacaacgt ctactcgatc
     2041 ctgaacccgt tgctggtgca ggtgatggcg cttggttatc acttcaattt ctatcgcaac
     2101 aagccgttac tgaaaaaagg cggcgtgatg atcgtgcatc acccgtgctt cgatgatttc
     2161 gatcaccagt ttcatccgag ctacatcgag tttttcaacc ggttgctgcc ggagacgcgc
     2221 gatgccttta cgttgcgtga gaagtatgag cgcgagtttg cgaacaatcc gagttatatc
     2281 gagatgtatc gtcgcgggaa tgcgtatcac ggcgcgcacc cgtttttcat gtggtactgg
     2341 ggtgagaatg ggcgccagca tgtcgggcgc gtgattgcgg cgggcgcgga gaatgcgcat
     2401 gtgccggcga ttctcggttg ggagcgtgct gacaatttga cggaagcgat cgcgatggca
     2461 aggagttaca tgggcaactc ggctgagatc acgatgttgc accagcccat gatcgggatc
     2521 gctgacatgg agtaacacgg attggcggat ttttataagg atttgcacgg atatgaaact
     2581 ctctccgact gaaatataca gagatcgaaa aatcttcctg attgggagca cgggttttct
     2641 cggcaaggtc acgctgagta tgttgctgca ccggttcccg aacatcggga agatctatct
     2701 cacagtgcgg gcgcgttcgc aggaggagtc ggagaatcgt ttctggaaca acatcatcac
     2761 cgcgccgcct tttgatccgc tgcgcgaacg ttacggcagc gcgctcgagg gttttgtcaa
     2821 cgataaggtg atcgtcaggg gcggtgacat cgctgagacg aacttcggct acagcgaaga
     2881 agaagcacaa gccatcgccg acgatatcga tgtcgtgatc aattcagccg gcaatgtgac
     2941 tttcaacccg acgctcgaga gcggactgcg tacgaacgtt gtcggtactc agaacgtcat
     3001 cgccttcgca cgccgcatga aacgcccggc gctcgtccat gtctcaacct gtttcgtcgc
     3061 gggtaatcgc tcgggcgcag tttgggagag cgattcggtg atcggctact tcccacgcca
     3121 tcgcgaacct gaaatgaagg gcgtgcagtt ttctgtggac caggagattg ctgactgcgc
     3181 caaactcgcc gaccgcgtga gagacgaagc acgtgacgcg atgatgacgg cgcgtttccg
     3241 ggagctcgcc cgcaaaagac tcaacgaaga aggtcgcgat atcgacgatc cggacgcgct
     3301 cgggctcgcc gtcgcccgcg aacgaaaagt ctggacgcgc acgcgcctca cggatctcgg
     3361 tgtagagcgc gcggcttact ggggttggcc caacatctac acctacacca aatcgcttgg
     3421 cgagcagctt gtcgcggccg agacagaaat ggtgcgtagt attgttcggc cgagcatcgt
     3481 cgagtctgcg atgtcgtacc cgtttccagg ttggaacgaa gggttcacga ccacggcccc
     3541 gatcatcttc ctgatccgta aaggccagac acagattccc gccgatccga agctcatcct
     3601 tgatatcacc cccgtggacc aggtcgcgtc ggtgatgctc gcggtctcgg cccaggcgtg
     3661 cgttgaagag ccgcgactcg ttttccaggc cgccaccggc gatgccaacg ccaacaacat
     3721 ggaacggatc atcggtcttt gcggactcga caagcgcaac tacttcgaag agaaagatag
     3781 cggagtaaag atcctcaata agatccaggg gcggatggag ccgcggcccg tcaccgtgtc
     3841 gaagttcgaa aacacgtcgg tgccgatgtt caacgcggcg gcgaagaaag cgtcgtcatt
     3901 cctcgacagt gtgcgcccgc gctggggcgg cggacgtgtc ggcgaagtga tcgatcgggt
     3961 gaaaagcagt gtcgagcgtg ttgaagagtt gacacgcgaa accaccgaag cgttcgacat
     4021 gtttcgcccg ttcacagtcg agaatgctta cgtgtttcgc tcggacaacg tgcgctcgct
     4081 cttcgatcgc atcagtgaag acgagcaatc gctgttgacg tggaatcccg aaaggtttga
     4141 ctggtacgac tactggctca acattcacct gcccggactc acgaagtggg tgttcccgac
     4201 actcgaagac gagatgcgtg ccgtaccgaa acgcgtctac acctatcgcg atctgctcga
     4261 gctgtttgag acatcaacga aacgtcacgc gacccgcgtg gcgatgcgca tcgagcgcga
     4321 cgggcgtaag gagcaataca cctacgctga catgcgcgag ctggcgacgc gagtcgctgg
     4381 atttctggcg aacgaaggaa tcaaacccgg cgatcgcgta atgctggtga gcagcaacgc
     4441 gcctgagtgg ggcatgagct acttcggcgt gctgaaagcc ggcgccacct gcattccctg
     4501 cgatcccgag agctcgaccg ccgagattct caacttcgcg cgtgccggaa atgccgcggg
     4561 tctgatctgc agtcaggccg tgcttgacga gcacccggat ctgcaagaac aactcagcac
     4621 tcgtctctgg acttacgacg acgtcttcgc aatgccagac gagcagactg aagacgaacg
     4681 catcgcactg ctgccgcaac gcgcccacgc gcaaagcgtc gcgtcactca tcttcacttc
     4741 gggcacgacg ggacagccga aaggcgtgat gctctcgcat cgcaatctga cgagcatggt
     4801 gtcgatgttg tcgtcagtgt ttgacatgac gactcgcgat ggcgtgttgt cagtcttgcc
     4861 gttgcatcac actttcgagt tctccaccgg ttttctgaca ccgctgagtc gtggcgcgca
     4921 gatcacttat ctgcccgagc ttacgagcga cgcgctcgcg cgtgcgatca agaatggtca
     4981 cgtgacgggc atggtcgggg tgcctgcgtt gtgggagctg ttgcaccggc gtatcaagaa
     5041 tcgtctctac gagcgcagtg actggatcgg taaagctgcc gataacctga tgtgggtcaa
     5101 tgcgtggctg cgcgataaaa ctcctttgaa tttcggacag atcctgttct atccgattca
     5161 cgaaggtctc ggcggccgca tccgctattt catcagcggc ggctcggcgt tgagcgagaa
     5221 gattcaacgc gactttcacg gtctgggctt cacgattctc gaaggctacg gtttgactga
     5281 ggcttcgccg gtgctgaccg tcacacgccc ggaaaatcag atgctaaccg gaactgtggg
     5341 caagccttta ccgggcgttg aggttcgcat tgccgatcct gatgccagcg gtgttggcga
     5401 agtgctcgcg cgtggtccaa acgtgatgct cggttactac gaagacgagc ttgccacgcg
     5461 caagacgctc gtcgatcgct ggctctacac gggcgacctc ggtcgtgttg acgacgatgg
     5521 gaatctgtat ctcgtcggtc gttcaaaaga gatcattgtc gatacgaacg gcaagaacgt
     5581 ctatcccgac gagctcgaag aaatttacgg aaactcggtt tacgtgaaag agctaagcat
     5641 cgtcggtctg gctgatggaa tcggtgagaa ggtggcatgt ctcgtcgtgc ccgatgatga
     5701 gtacgacatc tcgctttcgc gcgcggcgtt gcaccgtctc gtcgaagagc acttccgtga
     5761 agtctctgct tctctgcctt acttcaagcg cgtgaaagtt ttgcacttca cggacatcga
     5821 gctgccacgc accgcaacgc gcaaggtgaa gcgacgtgaa gtggtccaga tcatggagtc
     5881 gctggaggag agccagaagt ccggacgacg cggcagcgcg caggaaagtg cgaatgccga
     5941 ttcgttctgg ctgctcggac tggtttcgag tgtcgcgaat cgtccgcgat cggaagtttc
     6001 gaatgacacg aggttgagcg atcttgggtt cgatagtctg atgtttgtcg agctcgcgac
     6061 ggcaatcgag aacgcgggtg ggtcgatatc agcgccggaa cgttttaatg agattcagga
     6121 cgtgcgagag ctgttgacgg tggtcagccg gcagccgagc gccgcgccga agccggagac
     6181 gcgtgttgac gacagcaaga aggatgacga gattcacgtt ccttcgttcg tgaagacggc
     6241 aggaaacaag gccgccgact ttctccaaca atttctctac gatcagtttc tgcgaacgca
     6301 ttacgagggg cgctcgaaca ttccgccgca cacgaatttc ctcgtcgcgg cgaatcactg
     6361 ctcgcacctc gacatgggac tgacgaagat ggcgctcggt gagtttggta aagacctcgt
     6421 cgcactcgcg gcggctgatt atttcttcga cacgaagtac aagcgcgcgg tgatggaaaa
     6481 cttcacgaac ctcgtgccga tggagcgcac gggttcgttg cggcagtcgc tgcgtcacgc
     6541 gaagtcgttt ctcgatcggg gttacaacgc gctcatcttc cccgaaggca cgcgctcgat
     6601 gacgggcatc atggctgact tcaagccggt cgttggctat ctcgcgctgc acgcgcgcgt
     6661 cggcatcctg ccgatgtttc tcgacggcac gtatgaagcg atgccaaaag gcacgacgct
     6721 catcaagagc cgcgaggtag gcgcgcgcat cggtcgcctc cttgagatcg aagagctcga
     6781 ggagatgacg aagggcatgc cgcgcgccga agcgtacagg ctcatcgccg cgcgcgtcag
     6841 acacgaggtc gtaaacctcc gcgaccgcac acgctatgaa ttcgacgcga aggaactgcg
     6901 gaagctctgg aagagcgagc gcaaaaagga actggtgact gaggaagttg cggtctgatc
     6961 cgtggctctg atccgtgtca tctgtggtta agagagctat gccaaccaag aagcccggca
     7021 aacgcacagc gcggctgacc accatcggca cgaagcgtcg cgctgcgatc gagaagcgcc
     7081 agcgcgccgt cacactcatc actggcggca caggctttct cggctcacat ctcgtccgcc
     7141 aactgatcga agaaggcgcc agagacgtgc gcgtcatggc gacttcgatt cc
//
LOCUS       DASSFQ010000203        17679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_88965, whole genome shotgun sequence.
ACCESSION   DASSFQ010000203 DASSFQ010000000
VERSION     DASSFQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17679
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88965"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..2157
                     /locus_tag="VFH96_07160"
     CDS             415..2157
                     /locus_tag="VFH96_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973789.1"
                     /translation="MRVFCPEHQKAFFAPRQSPIKCENRGHLLGAIDFEGQSASPVEL
                     QWQYCCNCEHFCPVDFDQYGLQRCSVCTRRSSLLYLCDRCYVISFESNTPLQTKNFTL
                     TAEGVPQPSCPGCLRPASADLHEHTCDAVNASFITGLNTCPICEERLDIAPAFPSLVA
                     SYLKRTRAANKVSVTFDYESGSFVPVEDGEFVVVTNEDQAFVLPRASKFATKRDFYEF
                     YQDYYHCPNPDAGDVQILQPAFVKPADNGWSLVATGTLDVIKEQQPKKKPVVAPAIQP
                     TPIQQAPPRPAPAPKREEPEPPPVMTAAKKETPAVRTCTECGSQIEEKYFFCWKCGHS
                     MSNEQKPRQILSSAIDMDDELTLQHESRPHNSTVLPWSTSRAQEHPSSSRGSVLKLLA
                     IGGVAFTLLSLGAFGLFRSGTETASATTAPQQQQQVAQPASQPQPTAPITTEVSRNSP
                     AETTTQQVTPNPEDELKKLRERRMSAPASERPKVFQAFAKVEKQYPRDYRFPYERAKF
                     AAKGSKSGSQSEAFKALTTAAQKAISTGKAGEMLNGLESDKSGDFSRISQGRFEWSRL
                     VAALKRKDTSLLGE"
     gene            complement(2224..2517)
                     /locus_tag="VFH96_07165"
     CDS             complement(2224..2517)
                     /locus_tag="VFH96_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973790.1"
                     /translation="MANQSSESKVTTNHDEIRQWVEERGGHPARVKETESKNSPGLLR
                     IDYPGFSGGDSLEEITWDEFFTGFDKNKLAFLYQEKTKDGKESRFSKLIERDQ"
     gene            2634..3995
                     /locus_tag="VFH96_07170"
     CDS             2634..3995
                     /locus_tag="VFH96_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973791.1"
                     /translation="MNSWTKLLFLIPIISITSNAQQVVRITEPNAINPAEVSIAINPK
                     NPDNMIAASFQTGRPPKRRAGSYHYVTFDGGKSWKTVSTPNPTNLVQGDDVLAFSSDG
                     VAYHVHLSFDGIRLARPVRAENGMIVNVSKDGGNTWSDGTAAINHVNTVIPFEDKPGL
                     IVDNAAGSRFKGQVYLAWTRFDVYGSADPEHHSQIYFSRSTDQGQTFSMPFRISDTGG
                     DCLDSDNTVEGAVPAVGPNGEIYVVWAGPLGLVFDKSTDGGLTFGKDKVIGNIPGGWD
                     FGIEGLSRANGMPVTGVDLSNGPNKGTLYVNWIDARNGDPDVFVMSSRNGGETWTAPV
                     RVNDDALKNGKAQFFTWMAIDPADGSVNIVFYDRRDGKGTQTGLTLARSVDGGQTFVN
                     HKIDLPPFSINERAFFGDYSSISAYGGRVVPAFMHFVNDGGNLAISVALFRFKPGTQE
                     LVK"
     gene            complement(4070..5128)
                     /locus_tag="VFH96_07175"
     CDS             complement(4070..5128)
                     /locus_tag="VFH96_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET6973792.1"
                     /translation="MSQDSPTRVRHGEELPVDRLRDYLGAQQLTIEQFPSGFSNLTYL
                     LHADDRELVLRRPPIGAKIKTAHDMSREYRILSHLHPVYAKVPRPFSFCDDESILGAP
                     FYVMERVTGIILRAQKPHGLDLPPALMRRLSETFVDNLAEIHAIDYEAAGLGDLGSPQ
                     GYVKRQVEGWTKRYYNARTDDVPSIEKLADWLHENLPADSERPALIHNDYKYDNIVLA
                     SDDLARVIAVLDWEMATIGDPLMDFGTSLGYWVEATDPEEWQRYGFGLTSLAGSFTRT
                     ELLEHYAQRTGRRIDDPVFYFAYGLLKIAVIVQQIYFRYQKGLTRDPRFAQLGALVNA
                     CGDLAGRAIELKRVDGLG"
     gene            complement(5115..6314)
                     /locus_tag="VFH96_07180"
     CDS             complement(5115..6314)
                     /locus_tag="VFH96_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6973793.1"
                     /translation="MTDHHSEKLVGLLDRIKEFLHTNVYPLESDFLRRPFRELTPTLN
                     ATRQKVKALGLWAPHLPKEHGGLGLTLSEFALVSEELGRTPIGHYLFNCQAPDIGNME
                     ILLAHGTPDQKEKYFKPLASGEVRSCFSMTEPEHPGSNPTWMSTTAVKDGDDYVINGH
                     KWFTSSAEGASFAIVMAITNPESENPYKRASQIIVQIPTPGFQIVRNISVMGDEGSDY
                     ASHAEVRYENCRVPRSNLLGREGEGFAIAQERLGPGRIHHCMRWIGICERAFDLMCSY
                     AARRELSPGVLLASKQNVQEWIAESRAEINAARLLVLKTASGIDTKGAGEVREDISMI
                     KFFVAGILQKVIDRAIQVHGALGLTDATLLSYWYRHERAARIYDGPDEVHKMVVARSV
                     LKKYVSR"
     gene            complement(6333..7160)
                     /locus_tag="VFH96_07185"
     CDS             complement(6333..7160)
                     /locus_tag="VFH96_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973794.1"
                     /translation="MKLVSLALILLFAPTVAPQDHHARLDTLRAEGYEALYNLDYETA
                     RARFKKMIELAPDNPAGAECYASSLWVQQLNEAWKLKATLYSDKSSDTKSRVDSKQSE
                     EFRNWTRQAKQLSQARLKKDPKDVEALYFLGAAEGLEAAFAASVERKFMPAFRSGQDS
                     VEHHRAVLKLAPDFHDAELTIGLMNYVVGSLPLPAKMIAATMGVRGSKKRGLENLERV
                     AKEGKWARDLSRMMLIDLYKRERRFEDAAEMARQLSEKYPRNNLFKLQMTDALNHKL"
     gene            7339..7935
                     /locus_tag="VFH96_07190"
     CDS             7339..7935
                     /locus_tag="VFH96_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973795.1"
                     /translation="MKDEQIHELLYQALETELGGVQIYTTALRCAVNEDLKEEWEEYL
                     QQTKNHVTIMEDVLTKLDLDPDEETPGRKVVRTIGEALVQSMEMALAEGKPGAAQLVA
                     TEAVVLAETKDHQNWHLIGEVAKKAKGDVAKVLKEAYEEVEHEEDEHLYHTKGWSREL
                     WIESLGMKAVLPPPEEVKKVETAIGAARAEKARTQMAK"
     gene            complement(8040..8357)
                     /locus_tag="VFH96_07195"
     CDS             complement(8040..8357)
                     /locus_tag="VFH96_07195"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET6973796.1"
                     /translation="MTEDFYCDEVLSGRTPVRVVRETVNVLAYHHTRPFWPVHIVVIP
                     KRHISSLITLEESDDELLLELLRVVREVAREVLDEHGACRVLTNLGKYQDSKHLHWHV
                     SSG"
     gene            8456..9043
                     /locus_tag="VFH96_07200"
     CDS             8456..9043
                     /locus_tag="VFH96_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973797.1"
                     /translation="MYLRTILFSMLVLFCASTALAQRPGPEFWRKVGCEPLEPRTKLE
                     AIEDRHSTVVIKGFSTITTVEVRGVRIDAIEMRELGNVARAKGLVVVLREGGERPEDN
                     RAFVDYEEIEPLLNAIDAIGRVDESITKLAGFEARYKTQGDLQIAVFRQTARGTAVIL
                     TTGICNRATTTLTLDDLAKVRALIQEAKARLDEIR"
     gene            complement(9096..14975)
                     /locus_tag="VFH96_07205"
     CDS             complement(9096..14975)
                     /locus_tag="VFH96_07205"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HET6973798.1"
                     /translation="MKTIILSIFAFAFLLLNAVAQQPDYSQLKNDAETQYAQGSYARA
                     NEIYSRVDKSKLNPTEARWVEFRLADTSWRAEAATQTSDTTRLEQSQKQLEELIRVKD
                     KDTDRDLVWAEAHESLGDFFWRRGNTTWGNAWPHYQQALDWWAGQRDINLARDRYIRI
                     VFRAERPPIQNNDYYYTVYGTYIPLDVLENALKISTSANDISHLNFLIAMTMRYTGGS
                     YTARYRVPDQFEEALKAGKKSDWYDDALFHYAQWMGNYGSIQPLEDGQFQEQPDYEKA
                     LELYRRLLREFSRGETRYYDSALEQIKGITEASMSIAVSNIFLPDSELIFALNARNVR
                     RVDLALYKIDLTRDVHYISSVEEEDGENDPAPWIQRVPTAGRAPVKTWSKNIPDSQSH
                     QPFAEQSRIEGKLPVGAYLLEAKSGSLTSREIVLISDATLVLKSSSTQALAFFTNALT
                     GAPIANASVALWESYYLNGRWRWRRVRQTTDASGLARFQLRSNSDNRSLYATAAINDR
                     QAFASGYAYTTTRNEGWRIYAFTDRPAYRPKETLQWKFIARHANGGAYSTPANQTIEY
                     VISDPRGSKVASGKATLNAFGSSWGSLELGEQLPLGEYSITFSDAESKQQIGSARLFR
                     LEEYKLPEFKVAVKTPEQEGKKKAFRVGEKVEVEIQADYYFGGPVSNASVEVVVYQLP
                     FYHYWYWPREYSWYYDDMEQQRQNYYGGQGSVVKRETIKTDATGKAKVTFDTPNENYN
                     QDYEFNIEARVVDSSRREILSSDRVRVTRQRYYVYPRSEKYIYRPKDKVTVDIKALDA
                     NEQPVQTEGVVKVTRDYWWEIWIDPRGREVKGEELRQLRQRSEFFPPVTPRGQGRWRL
                     KFRGYQRDEILTQTLKTDAEGVAQLHFTPDREGYYSVAWQSNQGADPKRDRLLPPVKA
                     ETYVFVATNATTDLGYLREGLELVVDKDTFRAGQTAPVMINVPVPDRYVLFSVEGEDL
                     FSYQVVHVAGNTKLIEVPIEEKHIPNIYLSALMISDTQAYSDTKQVVVPPVERFLQVA
                     VNADREQYQPREEGILAISAKDAYGRPVSAEIALGLVDESVKYIQQDYAGDPRQFYYG
                     QKRQHTVQTTSTLDQKSYARYVEVEPGELRDSREDANEYDAMSVVRLKDGVANAPASV
                     GAGAKLESVGVLSVKEIRGLPVQGRVAADYAANAPSPTPLTGAQEPAVQVRSDFRSTI
                     LWLPDVKTDADGTATVKVKYPDSLTTWSATARVTTTGNQFGIGNSTTRTKQPLIVRLQ
                     APRFFVVGDQVTVSGVINNNTNEAMTVTPALNADGLSVTGLLIDGKPVSSAQTPIEVK
                     ANSEARVDWLVAVNHASEATLKVEARGSKYADAMEKKFTIFEHGIEKFVSRSGKMRGD
                     NVSIKLDIPKERRADSTKLTVQIAPSMATTMLDALPYLIDYPYGCTEQTMSRFLPAVI
                     TAKTLRDLGLKPETAMHKIFGGIEESSAAATHSNGRRDLKELDDITRKGLQRLYDFQH
                     ADGGWGWWKDGDSDHYMTAYVIWGMSLARQAGIDVKSDALDRAAAFLDHELVEEETNY
                     DAQAWMLHSLAVYHAMRKQTEVSKFQSVAFDNLWKNRDSLNAYTRALLALAAHNYGYR
                     DRAKTLVANLENGVKIDSRPDTSIVQQGAQSSDPSVMGTAHWGEDGVYWRWSEGGVEA
                     TSFVLRALLAIDPQNKLIEPATNWLVKNRRGAQWSNTRDTAIVVLTLNDYLRVSGEVQ
                     SAMSYQLVVNGNPVATKQVTVEDALSAPSKFAISRELLRDGANEISIVRSSGSGPIYF
                     SAAAEFFSLEEPLKPAGNEIFVKRQYFKLVNHPTLLKGFVTERVPLNDGETVKSGDRV
                     EVVLTVEAKNNYEYLLFEDLKPAGLEAVQIRSGESLYVRQLKSGGGFTGQQRWVYQEL
                     RDRKVALFIDKLPQGVWQMSYEMRAEVPGQFHALPVLGHAMYVPEIRTNGAETRLKVT
                     D"
     gene            complement(15110..16198)
                     /locus_tag="VFH96_07210"
     CDS             complement(15110..16198)
                     /locus_tag="VFH96_07210"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HET6973799.1"
                     /translation="MKTFFVYLSILLLPTVATAQMKPSESLTEKQASHFAALALKCVS
                     REFPNKPEHVINNASEVSSPKALHPAFYGCYDWHSSVHGHWMLVRLLKMFPNLPESAQ
                     VRAALGANLTAENIRAEVAYMKQPNRQSFERTYGWAWALKLAEELNSWNDADGKKWSE
                     NLQPLAETLTRSYLNFLPKQTYPIRTGVHPNTAFGLAFALDYAKAVGERQLEALLNER
                     SRTYYAKDSSYPGAWEPGGEDFFSAALMEADLMRRVMKPAEFATWFHRFLPDVATGGP
                     TALLQPAIVTDRSDPKLVHLDGLNLSRAWCMRSIAAALPANDPARKILAEAADKHAAA
                     ALAHVASGDYAGEHWLASFAVFLLSNEK"
     gene            complement(16208..16918)
                     /locus_tag="VFH96_07215"
     CDS             complement(16208..16918)
                     /locus_tag="VFH96_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973800.1"
                     /translation="MSFNVSAQRKSKKKPAPAQPPTELTKLREEYVKATNEYKASLGK
                     LLPFYENELKKAEEKLEVSKKLLAEGLIPKTQLEEYERAVAVAKQKIAETNRSMTSAD
                     EQVAAVVVEADANEQIAKNLRLAKQGLVRTAAFTRYTGAGGWNLGDSWKIQRFFYDTF
                     NKQLPIAVFGQGPIHDRWRLDHHNAMDIQLHPDGVEGRALLDFLQKNGIPYLAFRAAI
                     PGTATGPHIHIGRPSHRY"
     gene            complement(17008..>17679)
                     /gene="lepB"
                     /locus_tag="VFH96_07220"
     CDS             complement(17008..>17679)
                     /gene="lepB"
                     /locus_tag="VFH96_07220"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6973801.1"
                     /translation="KVPTGSMQNTITIGDHLHVNKFIFAPGKSLPFLPQREIKHGDII
                     VFKYPGNPYDPKRDDRPDNRPILTNYVKRVIGLPGDRIKIQGNNVYVNGEVVPEHHIT
                     ATDHNRKDPLEINDNPPRKPGELYDVYYKPGPADDDQEYDAFQGKDELVVPEASYFVM
                     GDNRNNSEDSRYWGFVPRDLVIGRAMFVYWSYDESAETTEGPFGIVVDFFKNTRWSRT
                     GTLVK"
BASE COUNT         3816 a         4952 c         4811 g         4100 t
ORIGIN      
        1 tttttcgggg ctcccgaaga aaatctcgcg tggttaaagg cgtgtagcgg caaaatgtca
       61 gacgaacaac tcgcctccat cgtcagtcgc tacgtccgcg accaacccaa cccgcaagcc
      121 aacctcaacg tcgtgacctt caacgccttc cgggaagcgt gccggcatca atagtttgat
      181 gtccgataag ctttaggttg tcgcgaccgt acgacaagct aaagcttatc ggacatcaaa
      241 actgattcac caatcttatt gcaaaatttg cgggatgacc gtaatctatc cacgaatccg
      301 caaacctctt cccgtttatt ttcatctgta ttccgtacac cccggcacgt atatgtcatg
      361 cttcgcgcca agtacaactt gaacggtcaa ttgtccctga agggagctac gaacatgaga
      421 gtattctgtc ccgaacatca aaaagcattt ttcgctcctc gacaaagtcc catcaagtgt
      481 gagaatcgcg gccacctcct cggcgccatc gattttgaag gccagtccgc gtctcccgtc
      541 gaattgcagt ggcaatactg ctgtaactgc gagcatttct gcccggtcga ttttgaccag
      601 tacggcttgc agcgctgttc tgtctgtacc cgcagaagct cgctcctgta tctgtgcgac
      661 cgttgctatg tcatcagctt cgagtcgaac acgccgctcc aaacgaagaa tttcacgctc
      721 accgcggaag gcgtgcctca accttcgtgc cccggctgtt tgcgacccgc atccgcagac
      781 ttgcacgagc acacgtgcga cgctgtgaac gcttcgttca tcacgggact gaatacgtgt
      841 ccgatttgcg aggaacgact cgacatcgcg cccgcatttc cgtcactcgt tgcgagttat
      901 ctgaagcgaa ccagggcggc taacaaagta agtgtcactt ttgattacga gagcggctca
      961 tttgtgccag tcgaagacgg tgagtttgtg gtagtcacca atgaagatca ggcattcgta
     1021 ctaccgcgcg cgtcgaagtt cgcgaccaag cgtgactttt acgagttcta ccaggattac
     1081 tatcactgcc ccaatccaga cgcaggcgac gtgcagattc ttcagcccgc gtttgtgaag
     1141 cctgctgata acggctggtc tctcgtcgcc accgggactc tcgacgtaat caaggagcaa
     1201 caaccgaaga agaaaccggt tgtcgcgccg gctatacagc caacaccgat acagcaagca
     1261 ccgccgcggc ccgctccggc tccaaagcgt gaagaacccg agccgccgcc ggtcatgacc
     1321 gccgcaaaga aggaaacacc cgctgtcaga acctgcacgg aatgcggatc tcagatcgaa
     1381 gagaagtatt ttttctgctg gaagtgcggg cattcaatga gcaacgagca gaagccgcga
     1441 cagatccttt cctctgcgat cgatatggac gacgagctca cactccaaca cgagtcacga
     1501 ccgcacaact cgacggtact gccgtggtcg acttcgcggg cgcaggaaca tccatcgtcg
     1561 agccgtggat cagttttgaa attgctcgcg atcggtggtg tcgcattcac gctgttgtcg
     1621 ctcggtgcgt ttggtttgtt ccgttcaggc acagagacgg cttcggcgac aaccgctccg
     1681 cagcaacagc agcaggtggc tcagcctgca agccagccac aaccgaccgc tccgatcacg
     1741 accgaagtct cgcgcaattc gcccgcggaa acgacgactc agcaagtgac tcctaacccc
     1801 gaagacgagc tgaagaaatt gcgtgaacga cgaatgtcag cacctgcttc ggagcgtccg
     1861 aaggtttttc aggcgttcgc aaaggtcgag aagcaatatc cgcgcgatta cagatttccc
     1921 tatgagcgcg cgaagttcgc ggcgaaagga tcgaagtcag gctcccaatc tgaagctttc
     1981 aaggcgttga ccaccgccgc acagaaagcc atttcgactg gcaaagcggg cgagatgttg
     2041 aatggtttgg aaagcgacaa gagtggagac ttcagccgca tctcacaggg acgtttcgag
     2101 tggagccgcc tcgtcgccgc actgaaacgc aaagacacga gcttgttagg cgagtaaatc
     2161 aaccacagat tacacggatt ttaaatccgg atttttatcc gtgtaatccg tggctaaagt
     2221 tttttactgg tctcgctcga tcagcttcga gaaacggctt tccttcccgt ccttcgtctt
     2281 ctcctggtag agaaatgcga gtttgttctt atcgaacccg gtaaagaatt cgtcccacgt
     2341 aatctcttcc agcgaatcgc cacccgagaa acccggatag tcgatgcgaa gcaatcccgg
     2401 actgttcttg ctctcggtct ctttgactcg ggccggatgc ccgccacgtt cttctaccca
     2461 ctgacgaatc tcgtcatgat tcgtcgtcac ttttgattca gatgattgat tagccatatc
     2521 tttcccctcc cataatcaat ttttgggatc tctgtatccg ttctttgtgc gcgagatgta
     2581 gcaaagatct aactattgac aggaccgcac gacttacgtg aaagtcactc actatgaact
     2641 cgtggaccaa attacttttt ctaatcccca tcatttccat aacatcaaac gcgcaacaag
     2701 ttgttcgcat cactgaaccc aacgcgatca atccggctga agtctcgatc gcgatcaatc
     2761 caaagaatcc tgacaacatg atcgcggctt cattccaaac aggtcgcccg ccgaaacgac
     2821 gcgctggcag ttatcactac gtcacattcg acggcggcaa gtcgtggaaa actgtatcga
     2881 cacctaatcc gacaaatctc gttcagggcg acgacgtgct cgcgttcagc agcgatggtg
     2941 ttgcgtatca cgttcacctc tcgttcgacg gcattcgact cgctcgaccg gtgcgcgccg
     3001 agaatggaat gatcgtcaac gtgtcgaagg atggtggtaa cacatggagt gacggcaccg
     3061 ccgcgatcaa tcacgtcaac actgtcattc cgtttgaaga taaacccggt ttgatcgtcg
     3121 acaatgcggc gggctcgcgc ttcaagggcc aggtgtatct cgcctggacc aggttcgatg
     3181 tttacggcag cgccgatccc gaacaccact cgcagatcta tttcagccgc tcgacggatc
     3241 agggacagac gttctcgatg ccgtttcgca tctctgatac gggcggcgac tgtctcgata
     3301 gcgacaacac ggtcgagggc gctgtgccgg cagttggtcc aaatggtgag atttatgtcg
     3361 tgtgggccgg gccgctcggt ctcgtgttcg ataagtcaac ggacggtggt ctgacgtttg
     3421 gcaaagacaa agtcatcgga aacattcccg gcggctggga tttcgggatc gaagggctga
     3481 gtagggccaa cggcatgcct gttacgggag tcgatctgag taatggtcca aacaagggca
     3541 cgctttacgt caactggatc gatgcgcgca acggcgatcc ggacgtgttt gtgatgtcgt
     3601 cacgaaacgg tggtgagacg tggaccgctc ccgtgcgtgt gaatgacgat gcgttaaaga
     3661 atggcaaggc acagttcttc acgtggatgg ctatcgatcc ggcggatggg tcagtcaaca
     3721 ttgtgtttta cgatcggcgc gatgggaagg gcacgcagac agggctgacg ctcgcgcgca
     3781 gcgttgatgg aggtcaaact tttgtgaatc acaagatcga cctgccgccg ttcagcataa
     3841 atgagcgcgc gttttttggt gattacagca gtatctcggc gtacggcgga cgcgtcgtgc
     3901 cggcgttcat gcatttcgtg aatgatggtg ggaatctggc gatatcggtg gcgctgttcc
     3961 gatttaaacc gggcacgcag gagttggtga agtgagtttt agacaggatt tacaagatgt
     4021 ttcagaattt acatgttcat cctgaaacat cttgtaaatc ctgtctcaac tagcccaatc
     4081 catccacgcg cttcaattcg atcgcgcgcc cagccagatc accgcaagca ttcacgagcg
     4141 caccgagctg cgcgaagcgt ggatcgcgcg tcagtccctt ctgataacga aaatagatct
     4201 gctgcacgat cacggcgatc ttcaacaatc catacgcaaa atagaaaacc ggatcgtcaa
     4261 tcctccgccc agtgcgctgc gcatagtgtt ccagcaactc ggtgcgcgtg aaactcccag
     4321 ccagactcgt caacccaaac ccgtatcgct gccactcttc cggatccgtc gcttcaaccc
     4381 agtaaccaag actcgtcccg aaatccatca acggatcgcc aatcgtcgcc atttcccaat
     4441 caagcaccgc gatcacacgc gccagatcgt ctgaagcgag aacgatattg tcatacttgt
     4501 aatcattatg gatcagcgct ggacgttctg aatcggcggg cagattttca tgcaaccaat
     4561 cagcgagctt ctcgatcgat ggaacgtcat ccgtgcgagc gttgtaataa cgcttggtcc
     4621 aaccttcaac ctggcgcttc acgtagcctt gcggcgatcc gagatcacca aggcccgcag
     4681 cttcgtaatc gatcgcgtgt atctcggcga gattgtcgac gaatgtttca gacagacgcc
     4741 gcatcaacgc cggtggaaga tcgaggccgt gcggtttctg cgcgcgcagg atgattcccg
     4801 ttacgcgttc catcacgtag aacggcgcgc cgagtatcga ctcatcgtca caaaacgaaa
     4861 atggccgcgg caccttcgca tagaccgggt gtaagtgcga gaggatgcga tactcgcgac
     4921 tcatatcatg cgcggttttg atcttcgcgc cgatcggcgg acgacgcagg acgagctcgc
     4981 gatcatccgc gtggagcagg taagtgagat tggaaaaccc tgaaggaaac tgttcgatgg
     5041 ttagctgttg cgcaccgaga taatcgcgaa gtcgatcgac aggtaattct tcaccgtgac
     5101 ggaccctggt aggactatct tgagacatac tttttcaaca cgctgcgcgc gacgaccatc
     5161 ttgtgtactt cgtctggacc atcgtagatg cgtgcggcgc gctcatggcg ataccagtac
     5221 gacaacaagg tcgcgtcagt cagtccgagc gcaccgtgca cctgaatcgc acgatcgata
     5281 accttctgca aaatgcccgc gacgaagaat ttgatcatcg agatatcttc gcgcacttcg
     5341 cccgcgccct tcgtgtcgat cccgctcgcc gttttcagca ccagcagccg cgccgcattg
     5401 atctcagcgc gactttccgc gatccactcc tgcacattct gtttgctcgc cagcaacacg
     5461 ccgggcgaca actcacggcg cgcggcgtag ctgcacatca gatcaaacgc gcgctcgcag
     5521 atgccaatcc aacgcatgca gtgatgaatt cttccgggcc cgagtcgctc ctgtgcgatc
     5581 gcaaacccct cgccttctct gcccagcaaa ttacttcgcg gcacgcgaca attctcgtat
     5641 ctgacttccg catgactggc gtaatcgctg ccctcgtcgc ccatcacgct gatgttccgc
     5701 actatttgaa atcccggcgt gggtatctga acgatgatct ggctcgcgcg cttgtaagga
     5761 ttctcgctct cgggattggt gatggccatc acgatggcaa agctcgcgcc ttcggcggac
     5821 gacgtgaacc acttgtgacc attgatgacg taatcatcac cgtctttgac agccgtcgtg
     5881 ctcatccacg tcggattcga accgggatgc tcgggttcag tcatcgagaa acaactgcgc
     5941 acctcaccac tcgcgagtgg tttgaaatat ttctctttct gatcgggcgt gccgtgggcc
     6001 agcaggattt ccatgttgcc gatgtcgggc gcctggcaat tgaagaggta gtgtccgatc
     6061 ggtgtgcggc cgagctcttc actcacgagc gcaaactccg agagcgtgag accgaggccg
     6121 ccgtgttctt tcggcaagtg cggcgcccac agtccgagcg ctttaacttt ctggcgcgtt
     6181 gcgttcagtg ttggggtaag ctcgcgaaag ggacggcgca ggaagtcaga ctcgagtggg
     6241 taaacattcg tgtgcaggaa ttccttgatc ctgtcgagga gtccgaccag tttctcgctg
     6301 tgatgatccg tcatgatgac ggcataaacg tatcacaatt tgtgattaag tgcgtcagtc
     6361 atctgaagct tgaacaggtt attgcgtgga tacttttccg ataactgtcg cgccatctca
     6421 gccgcatcct caaatcgcct ttctcgttta tataaatcga ttaacatcat gcgggagaga
     6481 tccctcgccc acttgccttc cttcgcgaca cgttcgagat tttcgagccc gcgcttcttc
     6541 gatccgcgca cacccatcgt tgccgcgatc atcttcgccg gtaatggaag tgagccgacg
     6601 acgtagttca tcaaaccgat cgttagctct gcgtcgtgaa agtccggggc gagcttgagc
     6661 acggcgcggt gatgttccac cgaatcctgt cccgagcgga atgcgggcat gaacttacgt
     6721 tcgacactgg cggcgaaagc tgcttcgagt ccttccgccg cgccgagaaa atagagcgcc
     6781 tcgacgtctt tcggatcttt cttcaaacgc gcttgagata gttgtttggc ctgacgtgtc
     6841 cagtttcgaa attcttccga ctgtttacta tcgacacgcg atttggtgtc gctgcttttg
     6901 tcgctgtaaa gcgtggcttt gagtttccat gcttcgttca actgctgcac ccacaaactc
     6961 gaggcataac actcagcgcc cgcgggatta tcgggcgcga gttcgatcat cttcttgaaa
     7021 cgcgcgcgcg cagtttcgta gtcgaggttg tagagcgctt cgtagccttc ggcgcgcaga
     7081 gtatcgagtc gcgcgtgatg atcctggggc gcgacggtgg gcgcaaatag gaggatgagc
     7141 gccaggctga cgagtttcat gtgagacatg attacaggat ttttaggatt tacaagatca
     7201 ggccagtgac agttatcaaa ctgccatacg atgtatcctg ttcatcctgg aaaccctgtc
     7261 atcctgtctc ataaaaatac aactccacac ctgatcattg cttattagcg tggcccctta
     7321 cttgctgaat cccacgccat gaaagacgaa caaatacacg aactcttgta tcaggcgctc
     7381 gagactgaac tcggcggcgt tcagatttac accacggcac tccgctgtgc cgtgaatgag
     7441 gatctgaaag aagaatggga agaatatctc caacagacca aaaaccacgt gacgatcatg
     7501 gaagacgtgc tgacgaagct cgatctcgat cctgacgagg aaacaccggg ccgcaaagtc
     7561 gtacgcacta tcggcgaggc cctcgtgcag tcgatggaga tggcgttggc tgaaggtaag
     7621 ccaggagcag ctcaactggt ggcgactgaa gcggttgtcc tggctgagac gaaggatcac
     7681 cagaactggc atttgatcgg tgaagtggcg aagaaagcta aaggcgatgt ggcaaaggtg
     7741 ctgaaagagg cgtacgaaga ggtggagcac gaagaggacg agcacctcta tcacactaaa
     7801 ggttggtcgc gcgagctctg gatcgaatcg ctcggcatga aggctgtgtt accgccgccc
     7861 gaagaggtga agaaggtgga gacggccatc ggtgccgcgc gtgcagaaaa agcacgcact
     7921 cagatggcga aatagtttta tcggcgggtc agccaaacaa agaagcacaa gagccacgaa
     7981 ttttatttgt gacttttgtg cctttttgtg tctgcctttc ttttgtgctt tttgtggccc
     8041 tagccggagc tgacgtgcca gtgcaggtgt tttgaatcct ggtactttcc gagattggtc
     8101 aacacacgac acgcaccgtg ttcgtccagc acttccctcg caacctcgcg cactacccga
     8161 agtaactcga gcagcaactc atcgtcactc tcttccagcg tgatcagcga tgagatgtga
     8221 cgctttggaa tgaccacgat gtgcacgggc cagaacgggc gcgtatggtg atacgcgagc
     8281 acgttgacgg tttcacggac gacgcgcacc ggcgttcgcc cgctcaatac ctcgtcgcag
     8341 taaaaatctt cagtcatcgg gcacggattt tacggattcg tgttattttt tggaactcac
     8401 ggcggacctg tgagtgaaga gtgctcaaca atcctactct cccttggagt ttcccatgta
     8461 tctaagaacg attctattct cgatgctagt gttgttctgt gcgagcacgg cgttggccca
     8521 gagacccgga ccggagttct ggcggaaagt aggttgtgag ccactcgagc cccgcaccaa
     8581 acttgaagcg atcgaagacc ggcacagcac ggtcgtgatt aaaggtttca gtaccatcac
     8641 gacagttgag gttcgcggtg tgcgtatcga tgcgatcgaa atgcgcgagc tggggaatgt
     8701 ggcgcgtgcg aaagggctcg tggtggtttt gcgagagggt ggcgagcgcc ctgaggataa
     8761 tcgcgcgttt gtggattatg aagagatcga gccgctgctg aatgcgattg acgccatcgg
     8821 gcgcgtggac gaaagcatca cgaagctggc gggattcgag gcgcggtata agacgcaggg
     8881 tgacctgcag attgcggtgt ttcggcagac ggcccgcggc acagccgtga tactcacaac
     8941 gggtatctgc aaccgcgcca ccaccacgct cacgctcgat gatctcgcga aggtgcgggc
     9001 cttgatacag gaagcgaaag cgcgactgga cgaaatccgg taacacggat tagacggatt
     9061 gaaatccgga tttaatccgt gttatccggg gctaattaat ccgtaacttt caggcgcgtt
     9121 tcggcgccgt tggtccgtat ctcaggcacg tacatcgcat ggcccaggac cgggagggcg
     9181 tggaattgac ccgggacctc ggcgcgcatc tcgtaactca tctgccagac gccttgcggc
     9241 agtttgtcga tgaacagcgc gacttttcga tcccgtagct cctggtaaac ccagcgctgc
     9301 tgccccgtga atccaccgcc tgacttcaac tggcgcacgt acaaactctc accactgcga
     9361 atctgcaccg cctccagtcc cgccggcttc agatcttcaa acagcaggta ctcatagttg
     9421 ttcttcgcct cgaccgtgag caccacttca acgcgatcgc cgctcttcac cgtctcacca
     9481 tcattcaacg gcactcgttc cgtaacgaac cctttcaaca gcgtcgggtg attcacgagt
     9541 ttgaaatact ggcgcttcac gaagatctcg ttccccgccg gcttcaatgg ctcttccaag
     9601 ctgaaaaact ctgccgccgc cgagaaatag attggaccac tgccactact ccgcacgata
     9661 ctgatctcgt tcgcaccatc gcgcaggagc tcgcgtgaga tcgcaaactt gctcggcgca
     9721 ctgagcgcgt cttctacagt gacctgtttg gtcgcgactg gattgccatt gacgactaac
     9781 tgataactca tcgcagactg aacttcacca ctgacgcgca ggtaatcgtt caaggtaagg
     9841 accacgatcg ccgtatcgcg cgtgttgctc cactgcgcgc cgcgacgatt cttcacgagc
     9901 cagttcgtgg ccggctcgat caacttgttc tgcggatcga tcgcgagcag cgcccgcaaa
     9961 acaaacgacg tcgcctcgac accaccttcc gaccagcgcc agtaaactcc atcctcgccc
    10021 cagtgcgctg ttcccatcac tgacggatcg gacgactgcg cgccttgctg cacgattgaa
    10081 gtgtcaggcc gcgaatcgat cttcacgccg ttttcaaggt tcgcgacgag cgtcttcgcg
    10141 cgatcgcgat agccgtaatt gtgcgctgcg agagcgagca acgcgcgcgt gtaagcattc
    10201 aggctgtcgc gattcttcca gaggttatcg aaagcgacac tctgaaactt gctgacttcc
    10261 gtctgcttgc gcatcgcgtg atacacggcc agcgagtgga gcatccacgc ctgggcgtcg
    10321 taatttgtct cttcttcaac cagctcgtgg tcgagaaacg ctgcagcgcg atcgagagcg
    10381 tctgacttca catcgatgcc ggcctggcgc gccagactca taccccagat gacgtacgca
    10441 gtcatgtaat gatcgctatc gccgtctttc caccagcccc agccgccatc ggcgtgttga
    10501 aagtcgtaca gacgctgaag acccttcctc gtaatgtcgt cgagttcttt cagatctctg
    10561 cgaccgttcg aatgagttgc cgcagcactc gattcctcga tgccgccaaa gatcttgtgc
    10621 atcgcagtct ccggcttgag accgagatcg cgcagcgtct ttgccgtaat cactgccgga
    10681 agaaaccggc tcatcgtctg ttcagtgcag ccgtacgggt aatcgattag atacgggagc
    10741 gcatcgagca tcgtcgtcgc catgctgggg gcgatctgaa cggtgagctt agtcgaatca
    10801 gcgcgacgct ctttgggaat gtcgagcttg atcgagacat tgtcgccacg catcttgccg
    10861 gaccgcgaga caaacttctc gatgccgtgt tcgaagatcg tgaacttctt ttccatcgca
    10921 tcggcatact tgctgccacg cgcttcgacc ttcagtgtcg cttcgctcgc gtgattcact
    10981 gcaaccagcc agtcgactct cgcttcgctg ttggctttca cctcaatcgg cgtctgcgcg
    11041 ctgcttacgg gtttgccgtc gatgagcaat cccgtcaccg acaagccatc ggcattcaac
    11101 gcgggcgtta ccgtcatcgc ttcattggtg ttgttgttga taacgcctga caccgtgact
    11161 tgatcgccga cgacaaagaa gcgcggcgcc tggagtcgca cgatcagcgg ttgctttgtt
    11221 cgggtcgttg aattgccgat gccgaattga ttacccgtag tcgtgacacg cgccgttgcg
    11281 gaccacgtcg tcaacgagtc aggatatttc acctttactg tggcagttcc atcagcgtca
    11341 gtcttcacgt caggcagcca cagaatcgtc gaacgaaagt cacttcgcac ttggactgct
    11401 ggttcttgag ctcccgtaag cggagtgggg gaaggcgcat tcgctgcgta atctgcggct
    11461 actctcccct ggactggtaa tcctctgatc tctttcacac tgagtacacc aactgactcg
    11521 agtttagccc cagctccaac actcgctggg gcattagcaa ctccgtcttt aagacgcacc
    11581 accgacatcg cgtcatattc attcgcatct tctctactat cgcgcagctc gcctggctca
    11641 acttccacgt aacgcgcata actcttctgg tccaatgtcg atgtggtttg cacggtgtgc
    11701 tgtcgctttt gaccgtagta gaactgtcgc ggatcgccgg catagtcctg ttgaatatat
    11761 ttcaccgatt catcaacgag gcccaacgcg atctcagccg acaccggacg tccgtacgca
    11821 tctttcgcac tgatcgcgag gatgccttct tctcgtggct ggtattgctc gcgatcggcg
    11881 ttcactgcaa cttgcagaaa tcgctcaacg gggggcacga cgacctgttt agtatcggaa
    11941 taagcttgag tatcgctgat catcaacgcg ctgagataga tgtttgggat atgcttctct
    12001 tcgatcggca cttcgatcag cttcgtgttg ccggcgacgt gaaccacctg ataactaaac
    12061 aggtcttcac cctcgacgct aaatagcacg taacgatcag gcacgggtac attgatcata
    12121 accggcgcag tctgtcccgc gcggaacgtg tctttgtcga cgaccagctc aagtccttcg
    12181 cgaagataac ccaagtccgt cgttgcgttg gtcgcgacga acacgtacgt ttcggctttc
    12241 accggtggca acaaccgatc gcgctttgga tcagcgccct ggttgctctg ccacgcgacg
    12301 ctataatagc cctcgcgatc tggcgtgaag tggagttgcg cgacaccttc agcatcagtc
    12361 ttcagtgtct gcgtgagaat ctcgtcgcgt tgatagccac gaaacttcag ccgccaacga
    12421 ccctgacctc gcggcgtaac tggcggaaag aattccgatc gctgtcgtag ctgtcgcagc
    12481 tcttcgcctt tcacttcgcg gccacgcgga tcgatccaga tttcccacca gtaatcacgc
    12541 gtgactttta ctactccttc ggtctgaacc ggctgctcgt tcgcatcgag cgctttgatg
    12601 tcgaccgtga ctttgtcttt cgggcgatag atgtactttt ccgaacgcgg gtagacgtaa
    12661 taacgctggc gcgtcacgcg cacacgatcg ctcgaaagga tctcgcggcg cgaagaatcc
    12721 accacacgcg cttcgatatt aaattcgtaa tcctgattgt agttctcgtt gggcgtgtcg
    12781 aacgtgacct tcgcttttcc ggtcgcatcg gttttgatcg tttcacgttt gacgacggag
    12841 ccctggccgc cgtagtagtt ctggcgctgt tgctccatgt cgtcgtagta ccaggaatac
    12901 tcacgtggcc agtaccagta gtgataaaac ggaagctggt aaacaacgac ttcgaccgat
    12961 gcgttcgaga ctgggccacc aaagtaatag tcagcctgaa tctcgacttc gactttctca
    13021 cccacgcgga atgctttctt cttgccttcc tgctcgggcg tcttcaccgc aactttgaac
    13081 tcaggcagct tgtattcttc gagtcgaaag agtcttgcgc taccgatctg ttgcttagat
    13141 tccgcgtcgg aaaatgtaat gctgtattcg cccaagggta gttgctcacc gagctctaac
    13201 gatccccacg agctgccaaa tgcattcagc gtcgctttgc cggaggcgac tttcgatccg
    13261 cgcggatcgc tgatgacgta ttcgatcgtc tggtttgcgg gcgtggaata ggcgccgcca
    13321 ttcgcatgtc tggcgatgaa tttccactgg agcgtttcct ttggtcgata agcaggacga
    13381 tcggtgaagg cgtagatgcg ccagccttcg tttcgagtag ttgtgtaagc gtaacccgaa
    13441 gcgaacgcct ggcggtcgtt gatcgctgcc gtcgcgtaga ggctgcgatt gtcagaattg
    13501 cttcgcagtt gaaagcgtgc gaggccgctt gcgtccgtag tttgacgcac acgacgccaa
    13561 cgccagcgac cattgaggta atagctttcc caaagcgcga cgcttgcgtt tgcgattggc
    13621 gcaccagtca atgcgttcgt gaaaaacgcg agcgcctgcg tcgaggacga cttgaggacg
    13681 agcgtcgcat cagaaatgag aacgatctcg cgcgatgtta gtgagccgct ttttgcttcg
    13741 agcaggtatg cgccgaccgg caacttccct tcgatccggc tctgctccgc gaaaggttgg
    13801 tgcgactggc tgtcgggaat gtttttgctc cacgtcttaa caggtgcacg accggcagtc
    13861 ggaactcttt gaatccacgg tgccggatcg ttttcgccgt cctcttcttc aactgagctg
    13921 atgtaatgaa cgtcgcgcgt caagtcgatc ttgtagagtg cgagatcgac gcgcctgacg
    13981 ttgcgcgcat tcaaggcaaa gatgagctcg gaatcgggca gaaaaatatt cgagaccgcg
    14041 atcgacatcg aagcttcggt gatgcccttg atctgctcca gagccgagtc gtagtaacga
    14101 gtctcacccc tggaaaactc gcgcagcaaa cgacggtaaa gctcgagtgc tttttcgtaa
    14161 tcaggttgct cctgaaactg gccatcttcg agtggttgga tcgagccgta gttgcccatc
    14221 cattgtgcgt aatgaaaaag cgcgtcgtcg taccagtcgg atttcttgcc ggccttgagc
    14281 gcttcttcaa actgatcggg cacacgataa cgagcggtgt aactgccgcc ggtgtaacgc
    14341 atggtcatcg cgatcagaaa gttcaggtgc gagatgtcgt ttgctgaagt gctgatcttg
    14401 agtgcgtttt cgagaacgtc gagcggaatg taggtgccgt agaccgtgta atagtaatcg
    14461 ttgttctgaa tcggcgggcg ttcggcgcgg aagacgatcc ggatgtaacg atcgcgggcg
    14521 agattgatgt cgcgctgtcc ggcccaccag tcgagtgcct gctggtagtg cggccaggcg
    14581 ttgccccagg tcgtgttgcc gcggcgccag aaaaaatcgc cgagtgattc gtgagcttct
    14641 gcccaaacga gatcgcgatc ggtgtctttg tctttcacgc gaatgagctc ctcgagctgt
    14701 ttctgggact gctcgagacg tgtggtgtca gaggtttggg tggcagcctc tgcacgccag
    14761 gacgtgtcgg ccagacgaaa ctcaacccaa cgcgcttctg tcggattcag tttgctcttg
    14821 tcgaccctgg aatagatctc gttggcgcgc gcgtaggagc cctgggcgta ctgcgtttcg
    14881 gcgtcgttct tgagttgaga gtaatccggt tgctgcgcga cggcgtttag cagcaggaag
    14941 gcaaaggcga agatagaaag gattattgtc ttcatggcgg ttatggaacg gtgaagtcag
    15001 gggagacttg cgtaagaact tttagccaca aaaggcacaa aaagcacaaa agagaattta
    15061 tgcccctgga gtaacggtaa accttttgtg ctttttgtac cttttgtggc tacttctcgt
    15121 ttgagagcaa aaacacggcg aacgaggcga gccagtgttc accggcgtaa tcgccgctgg
    15181 cgacgtgggc caaagcggcg gcagcgtgtt tgtctgcggc ctcagcaagg atcttgcggg
    15241 ccggatcgtt tgcgggaagc gcggccgcga tgcttcgcat gcaccacgcg cggctgagat
    15301 tcaaaccgtc gagatgcacg agcttgggat cgctgcgatc cgtgacgatc gccggttgca
    15361 gcaatgctgt tggaccaccc gtcgcgacgt ccggcaggaa ccggtgaaac cacgtcgcaa
    15421 actcggccgg cttcatcacc cgacgcatca agtcggcttc catcagcgcg gcggaaaaga
    15481 aatcttcacc gccaggctcc caggcacccg gatagctact gtcttttgcg taatacgtcc
    15541 ggctgcgttc gttgagtaac gcttccaact gccgctcgcc aactgctttt gcgtagtcga
    15601 gcgcaaaggc caaaccaaac gcggtgtttg gatgcacgcc ggtgcgaatc ggatacgtct
    15661 gcttcggaag aaaattcagg tacgacctgg taagtgtctc ggcgagcggt tgcaggttct
    15721 cggaccactt ctttccatca gcgtcgttcc acgaattgag ctcctcggcg agcttcaagg
    15781 cccaggccca accgtaagta cgctcgaacg actgccggtt cggctgcttc atatatgcaa
    15841 cttcagcgcg aatgttttca gccgtgagat tcgcaccgag cgccgcacgt acctgggccg
    15901 actccggcag gttcggaaac atcttcaaca gtcggaccag catccagtgg ccatgcacgg
    15961 atgagtgcca gtcgtaacaa ccgtagaagg ccgggtgcaa agccttggga ctgctgacct
    16021 cactcgcatt gttgatgacg tgttcgggct tgttcggaaa ctctcgactc acacacttca
    16081 acgcgagcgc cgcgaagtgg gaagcttgtt tctccgtgag agactcgctg ggtttcattt
    16141 gagcagtggc gacagttggt aacagcagga tggaaaggta gacgaagaag gtcttcataa
    16201 gtttcgatca gtaacggtga gatggtctgc cgatgtgtat gtgtggacca gtcgcggtgc
    16261 ccggaatggc cgcgcgaaac gccagatatg gaattccgtt cttttgcaga aagtccaaaa
    16321 gcgcgcgccc ctccacgccg tccggatgaa gctggatatc cattgcgttg tggtggtcca
    16381 ggcgccagcg atcgtggatt ggtccctgtc cgaacactgc gatgggaagc tgcttgttga
    16441 aggtgtcgta gaagaagcgc tgaattttcc aggagtcgcc gaggttccag ccaccggctc
    16501 ccgtgtagcg tgtgaaagct gcggtacgca cgagaccctg ctttgcgagt cgcaggttct
    16561 tggctatctg ctcattggca tccgcttcca cgactacggc ggcgacttgc tcgtcagcgc
    16621 tcgtcatcga gcgattagtt tcagcgatct tttgtttcgc aaccgccacc gcgcgctcgt
    16681 attcttcgag ctgcgtcttg gggatcaatc cttcggcgag tagctttttc gatacctcga
    16741 gcttctcttc cgccttcttc aattcgttct cgtaaaacgg cagtagcttt ccgagactgg
    16801 ctttatactc gttcgtcgcc ttgacgtatt cctcacgcag ctttgtgagc tcagtcggcg
    16861 gctgcgccgg cgcgggcttc ttctttgact tccgttgcgc cgaaacgtta aaagacacta
    16921 gcgccacaag cactaacacc tgcacacaga aactaacccc gtaaccccta aacatcttct
    16981 tatgtgccta ttgtgccttt tcgtggccta cttgacgagt gtgccagtgc ggctccagcg
    17041 cgtgtttttg aagaagtcga cgacgatgcc gaaaggccct tcggtggtct cggcgctctc
    17101 gtcgtaggac cagtaaacaa acatcgcacg accgatgacg agatcgcggg gcacgaatcc
    17161 ccagtaacga ctgtcttcac tattattccg gttatcgccc atcacgaaat aactcgcctc
    17221 aggtacgaca agctcatcct tgccctggaa ggcgtcgtac tcctgatcgt catccgcagg
    17281 tccgggtttg taatagacgt cgtagagttc accgggtttg cgcggcgggt tgtcattaat
    17341 ctccaaagga tccttgcgat tgtgatctgt ggcagtgatg tgatgctcgg gaacgacctc
    17401 gccgttaaca tagacgttgt ttccctgaat cttgattcga tcgccaggca gaccaatcac
    17461 gcgcttcacg tagttggtga gtatcggccg gttgtcaggc cggtcgtcgc gtttgggatc
    17521 gtaagggttg ccgggatact tgaagactat gatgtcgccg tgcttaatct cgcgctgcgg
    17581 cagaaacggc aacgactttc cgggtgcaaa gatgaatttg ttgacgtgca ggtgatcgcc
    17641 gatggtgatc gtgttctgca tcgatccggt cggcacctt
//
LOCUS       DASSFQ010000204         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_241251, whole genome shotgun sequence.
ACCESSION   DASSFQ010000204 DASSFQ010000000
VERSION     DASSFQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241251"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VFH96_07225"
     CDS             <1..1049
                     /locus_tag="VFH96_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET6973802.1"
                     /translation="RLSGIDALRPFTLAKSPLLRVSLLRLSQQEHIAIVSMHHIISDG
                     WSIHLLIEEVTALYEAYRSGKPSPVPDLPVQYGDFAHWQTELLQPGSAVLESQLAYWK
                     EQLRDVAELKLPLDRPRPAVYDPRGALLPLRYSAELSQALKKLSRREDVTLFMTLLSA
                     FNVLLHSLSGQEDIVVGAPIANRTRVEMEKLIGFFVNMLVLRTDLSGDPTFRELLQRT
                     REVMIGAYAHQDVPFAKLVGELNVKRDASRNPLFQVAYIFDNTSVQTPELNGLRLSQF
                     NFEVNTAPFDLSLFLTETADGLRGSVMYNTALFDEQTIRRMLDHHETLLERVVADPNQ
                     RLSSFNFLAKALAG"
     gene            1115..2116
                     /gene="sbnA"
                     /locus_tag="VFH96_07230"
     CDS             1115..2116
                     /gene="sbnA"
                     /locus_tag="VFH96_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HET6973803.1"
                     /translation="MNDGVLSAIGKTPLVRLSRVIPDARFKLYAKLEGLNPGGSTKDR
                     PAFSIIRHAIDNGDIQPDTVVIESSSGNMGIGLAQACKYYGLRFICVVDPKATVQNVR
                     LLKAYSAEVDVVQEPDKRTGEYLQARLNRVQSLLASIENTFWPNQYSHRYNPIAHYQT
                     MDELVTELGGQLDYLFCATSTCGTIRGCAEYARDHKLATRIFAVDAVGSVIFGGTNGK
                     RLIPGHGAAVRPALYKADVAERCIHITDLESVVGCRRLVRDEAILAGGSSGAVLMAIE
                     QMKNEIKPGATCAAIFADKGERYLDTIYSDSWVEEHFGNVAHLWQNEKELPVMVATG"
     gene            2131..>3554
                     /locus_tag="VFH96_07235"
     CDS             2131..>3554
                     /locus_tag="VFH96_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET6973804.1"
                     /translation="MSQKKAAVATDRVIPRRADPEAAIPLSFAQQRLWFLDRLNPQSA
                     FYNVPMAIRLRGELNVKAFERGLNELLQRHESLRTSFSVRDDQAVQVIAPSTEFHLPL
                     TDLTHLPEDEAEAEVRRLANADAQLPFDLRRAPLMRAALLRLSTRHHILLLSLHHIVT
                     DGWSNAVLYRELTTLYHAFNARSKPQLPELPIQYADFAVWQRHWLQGEVLEGHLSYWR
                     EQLAGAAPAINLPTDHPRPAVEGFRGAKQYVRMPAKLLRSLKELAQQEGVTLFMTLLA
                     AWATLLARYSGEEDIIVGTPIANRTRTELEGLIGFFVNSVVLRTSLKGDPSFRELLGR
                     VREVTLGAYAHQDLPFEKLVEELQPERELNHNPLFQVSFALQNAPQGAIEVGGLEMSG
                     VETDRRTARFDLEFNLWERGQELTGSIIYSTELFEAETIERLMSHYERLLTEVVAHPE
                     QRVSELKLLTETEERQLVFEWNDT"
BASE COUNT          753 a          961 c         1073 g          767 t
ORIGIN      
        1 ggcgtctgtc cgggatagat gcgttgcgcc cgtttaccct cgcgaagtca ccattattgc
       61 gcgttagtct gttgcgtttg agccagcagg aacacatcgc catcgtctca atgcaccaca
      121 tcatttccga cggctggtcc atacacctgc tcatcgaaga ggtaacggcc ttgtacgaag
      181 cataccggtc cggaaaaccg tcgccggtgc cggatctgcc ggtgcaatat ggcgatttcg
      241 cgcattggca gacggaactg ttacagccgg gaagcgcggt gctcgagtca caactagcct
      301 actggaaaga acagcttcgc gacgtggccg agctgaagct gcccctcgat cgaccccgac
      361 ccgccgtcta cgatccgcgc ggagcgctcc tgccgttgcg ctactcggct gagttgtcgc
      421 aggcgttgaa aaagttgagc cgtcgtgaag acgtgacgct ctttatgacg ctgttgtccg
      481 cgttcaatgt tttgttgcat tcgctaagcg ggcaggaaga catcgtcgtg ggcgctccga
      541 tcgcgaatcg cactcgcgtc gagatggaaa agctgatcgg attttttgtg aacatgctgg
      601 tcctgcggac agatctgagt ggcgacccga cttttcgcga gctactgcag cgcactcgcg
      661 aggtcatgat cggtgcgtac gcgcaccagg atgtgccgtt cgcgaagctt gtaggagaac
      721 tgaacgtgaa gcgcgacgcg agtcgtaatc cactctttca ggtcgcctac atctttgata
      781 acacctcggt gcagacgccg gagttaaacg gcctcaggtt gagtcagttc aattttgagg
      841 ttaatacagc gccgtttgat ttgagcctgt ttctcaccga gactgcggac ggcctgcgtg
      901 gttcagtgat gtacaacaca gccctcttcg atgagcagac gatccggcgc atgctcgatc
      961 atcacgagac actgcttgaa cgagtggtag cggatccgaa tcaacgcctc tcgagtttca
     1021 acttcctcgc caaagcttta gctggttaat cctcaaacta aaactatcgg tcctatatgt
     1081 cctgtaggac ttatttgtaa ggttgccaaa taaaatgaat gacggtgtgt tgtcagccat
     1141 tggtaaaaca ccgctggtga gactgtcgcg cgtgataccc gacgcgcgtt tcaaactcta
     1201 cgccaagctg gaaggattga atcccggcgg cagcactaaa gaccgtcctg ccttcagcat
     1261 cattcgccac gcgatcgaca acggcgacat ccagccggac acggtcgtga tcgaatcgag
     1321 ctccggcaac atgggcattg gcctggccca ggcttgcaag tattacgggt tgcgctttat
     1381 ctgcgtggtc gatccgaaag ctaccgtgca gaacgtgcgt ctgttgaagg cttacagcgc
     1441 cgaggtcgat gtcgttcagg agcctgacaa aagaaccggc gagtacctgc aagcgcgact
     1501 caatcgcgtc cagagtctgc tcgcgtcaat tgaaaatacg ttctggccca accagtattc
     1561 acatcgttac aacccgatcg cccactacca gacgatggac gagcttgtta ccgagcttgg
     1621 agggcagctt gattatctgt tttgtgcgac gagtacatgc gggacgattc gtggttgtgc
     1681 ggagtatgca cgcgatcaca agttggcgac gcgcatcttc gcagtcgacg ccgtcggcag
     1741 tgtgatcttt ggcggcacga acggcaagcg actgattcca gggcacggcg cagcagtgcg
     1801 tccagcgctg tataaggccg atgtggcgga gcgttgcatt cacatcacgg atctcgagtc
     1861 tgttgttggt tgccggcgtc tggtgcgtga tgaagcgatt cttgcgggtg gatcgtcagg
     1921 cgccgtgctg atggcgatcg agcagatgaa gaacgagatc aagccgggcg ccacctgtgc
     1981 cgcgatcttc gccgacaagg gcgaacgcta tctcgacacg atctattccg atagttgggt
     2041 ggaagaacac ttcggcaacg tggcccatct ctggcagaac gagaaagagc tgccggtgat
     2101 ggtcgctacg ggttgatgtc aaaataaaca atgagtcaga aaaaagcagc cgtcgcaacg
     2161 gatcgcgtca ttcccagacg agctgatccg gaagcggcaa tcccgctgtc gtttgcgcaa
     2221 caacggctct ggtttttaga ccgcttgaat ccccagagcg cgttctacaa cgtgcccatg
     2281 gcgattcgac tgcgtggtga gttgaacgtg aaagcgttcg agcgcggcct gaacgagctg
     2341 ctgcagcgtc acgaatcgct gcgcacttca ttctccgttc gggacgatca ggccgtgcaa
     2401 gtgatcgcac catcaaccga gttccactta ccgctgacag atctcacgca cctccccgaa
     2461 gacgaggccg aagctgaggt gcgacgcctg gcgaatgccg atgcgcaact tcctttcgat
     2521 ctacgccggg cgcccctgat gcgcgctgcg ttgctgcgac tgtcaaccag gcatcacatt
     2581 ctgctgctca gcctgcatca catcgtgacc gacggctggt ccaatgctgt gctgtatcgc
     2641 gagctcacga cgctttatca cgctttcaac gcccgttcga agccgcagtt gccggagttg
     2701 ccgattcagt acgccgactt tgctgtgtgg cagcgccact ggctgcaagg cgaagtgctg
     2761 gaggggcact tgagctactg gcgtgagcaa ctcgcgggcg ccgcgccggc gatcaatctg
     2821 ccgacagatc atccgcgccc ggcggtcgag ggatttcgtg gcgctaagca gtatgtacga
     2881 atgccggcaa agctgttgcg gtcgttgaaa gaattggcgc aacaggaagg cgtgacgttg
     2941 ttcatgacgc tgctggcggc gtgggctaca ttactcgcgc gctacagtgg tgaagaagac
     3001 ataattgtcg gcacgccgat cgcgaaccgg acgcggaccg agctggaagg gttgattggt
     3061 ttcttcgtga actcggtggt gttgcgcacg agcctgaagg gcgatccgag ttttcgcgag
     3121 ttgctgggcc gggtgcgcga agtgacgctg ggtgcatacg ctcatcagga cttgccgttt
     3181 gagaagctgg tggaagagtt gcagccggag cgtgagttga atcataaccc gctgttccag
     3241 gtttcgtttg ccttgcagaa cgcgccgcag ggtgcgatcg aggtgggcgg cctggagatg
     3301 agcggtgtcg agactgaccg ccggaccgca cgcttcgatc tcgaattcaa tctgtgggag
     3361 cgcgggcagg aactaaccgg ttcgatcatc tacagcacgg agttgttcga agcagagacg
     3421 attgagcggc tgatgagtca ctatgagcgg ttgttgactg aagtagtggc ccatccggag
     3481 cagcgcgtat cagaactgaa gctgctgact gaaacggaag agcggcaact cgtgttcgag
     3541 tggaacgaca cgaa
//
LOCUS       DASSFQ010000205        17005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_89488, whole genome shotgun sequence.
ACCESSION   DASSFQ010000205 DASSFQ010000000
VERSION     DASSFQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89488"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..577
                     /locus_tag="VFH96_07240"
     CDS             164..577
                     /locus_tag="VFH96_07240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973805.1"
                     /translation="MTVETSELSLPSRIDTIATVAAAVADFVGRSGISDDAAFGIDMA
                     VREAVTNAVLHGNRQDEDKTVDITLKSSPDAVEISVHDQGPGFNPETVPDPTAQENIL
                     KASGRGIFFMRSFMDEVDWLIRPGGGTTVRMLKRR"
     gene            607..957
                     /locus_tag="VFH96_07245"
     CDS             607..957
                     /locus_tag="VFH96_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6973806.1"
                     /translation="MAELNINERQAGDVTVLDLSGKITIGEGSVALRTAIRRLLEEGK
                     KRILLNLAGVSYVDSSGIGELVSSYTAIGKEQGGQLKLLNLTQKIRDLLTITKLLTVF
                     DVYENEAEALNSYK"
     gene            970..1317
                     /locus_tag="VFH96_07250"
     CDS             970..1317
                     /locus_tag="VFH96_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6973807.1"
                     /translation="MAELDVKERQAGDVTILDLSGEVRIGEGAVSLRDSIRNLSDQGK
                     KKVLLNLAGVKYMDSSGVGELIANYTTVTRQGGQLKLLSLTDRIQNLLVITKLLTVFD
                     SFDNEAEALKSFE"
     gene            1415..1900
                     /locus_tag="VFH96_07255"
     CDS             1415..1900
                     /locus_tag="VFH96_07255"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HET6973808.1"
                     /translation="MFRAAFRRLLAIDDPPERTALAFSIGVFIAFSPFLGLHTIMATA
                     LAFLFRFNKIAIYTGTFVNNPFLTLVPIIIASYAVGAFIIGRPLRIPDEGLELLTHPR
                     IFSGDYYKLLFVQSWNHIVWPFSVGGMVLSVVCSALAYPLTLRALRAYRARKKPKIHT
                     E"
     gene            1955..3721
                     /locus_tag="VFH96_07260"
     CDS             1955..3721
                     /locus_tag="VFH96_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973809.1"
                     /translation="MASSQTVQREPAKPQPSPPVQQRPQTGFEVSEYGVDFQADPRLI
                     LVMAALDTAGLDATPAGRQPSVFRTKLRKDLANLDPALREKMKTFYERNRLPAPATPA
                     DQAARYVSLALALGPSPTFDAPERSEDLPAGLLEVLDFAPLVQEFARRSGIDEHMVEY
                     VRAYQAEGDRLRAPVAEMVRGLLTYLHTRPITSSAERVEVKNPNKKSKEKAYSFRQKE
                     RRFLILPDLLAARGAINFRIIGDDYYAVVPEGTDPSSSELRRAYLQYVIDALVLRFNK
                     DIALRRDQIKQLLNERQQAGAQVTPDVFLSVSRSLVTAADARYEEMRRLEVLGRDARA
                     QLAAAKTEAERASIGKTATEEMKAIQDETVARLAAEYEKGAVLSFYFADQLKGIESAG
                     FDLANFFPDMIASFDPAREAKRPSEYAETVQRATAAREARTAARRSASEVNVEASGKE
                     AALVRDLSSIEDTLRNKDYNEAENRLREMLKEYPREPRIFFALGQTASLAASDATDEN
                     VQAERLNRALGHYRMAVAASSPETDKAIMSRAHEAMGRINMFLENTAEAAKQFDEAIK
                     IGDVRGGAYREAVEARKKLNQP"
     gene            complement(3775..5190)
                     /locus_tag="VFH96_07265"
     CDS             complement(3775..5190)
                     /locus_tag="VFH96_07265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET6973810.1"
                     /translation="MNRNNFFIHLALALVLVLVAAFAGQLSRWRKQWRSHIGPIPATE
                     KKRATTTEEIALSETRSFGRPEVLVKFKTGVSQETIERLTALRHDRVEDRIENVEGLE
                     AIDDLDNADAATVVNEYQQLPEVEYAEPNFEIELDATESSGPLVPILPQDPQFNDQWA
                     LANSGQRGGKQGADISATLAWATTTGSDKVVVAVLDSGVDYTHEDLIQNMWMRPESMA
                     PYHDNELGTIDDENGFNAIDTASDPMDENGHGTHCAGIIGAEGENNIGIAGVNWKVKI
                     MPLKFMNAGGFGTTKDAIEAINYVIDRKKAGVNVRIISASWGSTQRSRALEDAIRKAY
                     ENDILFVAAAGNSTVNNDRTPHYPSSYNVPNVVSVAALDRHDELARFSNWGVKSVAIA
                     APGVDILSTWLGDAYEEKSGTSMATPVVSGVAALILAEHPRMSVDDLKKKLLASTDPI
                     VALKGKTVTGGRINAAKALAN"
     gene            complement(5209..5484)
                     /locus_tag="VFH96_07270"
     CDS             complement(5209..5484)
                     /locus_tag="VFH96_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973811.1"
                     /translation="MAIDKNSAVENARNDLAKRLNVNAADIKEKSVEDDDFPDMALGA
                     AEDGEMSGQMITSGWRIKLEAQGKTYEYRADKNQVRAFNFKGKNHRI"
     gene            complement(5639..6367)
                     /locus_tag="VFH96_07275"
     CDS             complement(5639..6367)
                     /locus_tag="VFH96_07275"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET6973812.1"
                     /translation="MTRRRFHAPPTAFTQTTVTLTADEARHLRDVLRLKAGDEVYVFD
                     GRGREFRCSVLNTKRDSAELRIDAEAQPAKPESQLQLNLCVALLKGEKFDLVVQKTTE
                     LGVVKITPLITRHADIHLRDQSDAAKRVGRWQRIALEAVKQSGRAFVPEIALPVPFET
                     ALDVEGVGVMFSERGGESFESLTGASSITALVGSEGGWADEEIDAARARNFHIVTLGG
                     RVLRAETAAIAVTVLMQHRFGDLR"
     gene            complement(6372..7217)
                     /gene="prmA"
                     /locus_tag="VFH96_07280"
     CDS             complement(6372..7217)
                     /gene="prmA"
                     /locus_tag="VFH96_07280"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET6973813.1"
                     /translation="MTSAKEKPSGWYALDVEIDGAAHEAVEYALMEAGALGTETTDHG
                     VTAYFAETPNRERVRNELFEALRIYELPSSSVRDMNLREVADRDWLEEWKQSWQPVEI
                     GRFIIAPPWSNISDVHDRLVIRIEPGMAFGTGTHETTRLCLDAIQKHFAGGSFLDVGT
                     GTGILAIAAAKLFPSAHIEACDNDETAIAIARENAELNEVQINFWAGSIDDSTASADL
                     VVANLTADVITSIMPALVGVTCGKLILSGILETQLEMVQASLHESGIDEFEITQDGEW
                     IALIV"
     gene            complement(7214..8104)
                     /gene="era"
                     /locus_tag="VFH96_07285"
     CDS             complement(7214..8104)
                     /gene="era"
                     /locus_tag="VFH96_07285"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET6973814.1"
                     /translation="MTHSGYVALIGRPNAGKSTLLNRLVGQKIAAVSDKPQTTRFRIQ
                     GVITKPEGQIVLVDTPGVHQPGYELNRRMMAAVQEALMGVDLVCLIRDVSVSTGNGDR
                     FVLDLIKRSEKPALLLLNKIDKLEEKSALLPLIDWYRNEHEWAEIIPISALKDEMTDV
                     LIESALKHLPEGEPIFSEDELTDQSLRVLVSEIVREKVLHATADEIPYVTAVVTERFE
                     EIREDFTRINCVIVVERTTQKKIIIGKGASRLKDIGIRARQDIEALLGHRVHLELFVK
                     VEQDWRNKEQLLDEYGLSGN"
     gene            complement(8135..9442)
                     /gene="pepT"
                     /locus_tag="VFH96_07290"
     CDS             complement(8135..9442)
                     /gene="pepT"
                     /locus_tag="VFH96_07290"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET6973815.1"
                     /translation="MKSIIAFVLCLVCVGVANTQLKPPVESAMDRFLRYVKIDTQSQE
                     DQTTVPSTRKQLNLANLLAKELTALGAQNVRVSEFGIVYANVPGNLADNSKVPVIGFI
                     AHMDTSPAVSGENVNAIIHKNYQGGDITLPKDPTQVIMVDKTPALKTLIGDDIITADG
                     TTLLGSDDKSGCAEIMTMIDILRQNPQLKHGTLAIAFTPDEEVGGGIEKFDVKGFGAK
                     FAYTVDGEELGEISDETWSARTATVTFQGKSTHPGTAKGIMVNAMYAIADYLNSVPHD
                     ILPETTEGRVGFIHPYAGVIDVEKSTLKILLRDFDISGLDAKEKLLRDMVAKTSDKFP
                     QVKITIDVEENYKNMKEVLKNYPELTNNAMEAAKRAGLKPFLKPIRGGTDGSRLTFEG
                     LPTPNLFTGGGNFHGKLEYNSRRGLEKSTETLVHLVQIFAEKS"
     gene            complement(9454..10170)
                     /locus_tag="VFH96_07295"
     CDS             complement(9454..10170)
                     /locus_tag="VFH96_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6973816.1"
                     /translation="MNTRINRVDRAYEFADLSSYSFLDRVKIRAADLVFYLLINLIGL
                     TARFEVIGWENHDKAEENGGLPIYVFWHDRIFLTTYWWRKRRIVVMTSRSFDGEYIAR
                     FIQRFGYGAVRGSSTRGGVGAIIEMARLMRAGCTTAFTIDGPKGPRHIAKMGAVLLAK
                     KSGQPIVPVTMALARYWTTPSWDLFQIPKPFTLARVYVAPPIYVPADADEATLAAKRN
                     ELQQTLDDLNHQSEEWRKSL"
     gene            complement(10187..11110)
                     /locus_tag="VFH96_07300"
     CDS             complement(10187..11110)
                     /locus_tag="VFH96_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6973817.1"
                     /translation="MAKHGKLRTYLEYAPVKLVLMTLGRLPPRMSYGIGRSLGKIGYL
                     LAGNLRRTGAINLRLAFPEKTDEERAALLRECFESLGRELGLFSQMATRSREEIRQLI
                     EPVGWENLENARAERGDRLIYYTGHLGAWEMTSFGISLLGYPFTFLTRRLDNVKIEEL
                     VDGVRTKFGNRTIDKLSAARSMLKLLRSGEHALGLLPDLNTLDDEAIFVDFFGVPAAT
                     TFVIAKLAVRTNTPLVPFFAPWSEEKGKYLLIAEPPIPAECTGDEEADVRRVTIEITR
                     RLENQIRQFPGQWLWIHKRWKTRPPGEPKIY"
     gene            11280..12128
                     /locus_tag="VFH96_07305"
     CDS             11280..12128
                     /locus_tag="VFH96_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973818.1"
                     /translation="MEKRRILIVDNNDELRVLLEQALGRLGHEVIVTGEREKALRRDD
                     LDSFDLIISDLSEAEVNGEPIGELHRRHLVTELNGQKNGPGVIKAFKMGAANYLRVPY
                     NRDELREIVEQTLANKLRYCDDTSAFSHVHEKIEFELPSDLALMNGVLDFLQERVAKL
                     GLIRPERSNLFVALDEAFVNAVKHGNKNDLTKLVKITAELSPHEASFTVEDEGQGFDI
                     REIPNPCDPENLFRTSGRGVLLIYNIMDEVEYNAQGNRVKMVKRPDAPVKAQLVDPNT
                     PDDKHV"
     gene            12070..13716
                     /locus_tag="VFH96_07310"
     CDS             12070..13716
                     /locus_tag="VFH96_07310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6973819.1"
                     /translation="MLRLKLNSLTQIPLTTNTSETFPFIQSSALSGTASFWRAWRWWL
                     PPAVVALALVLYFVDPFIGDWDGMDYTMLSLAGYPSSMALGRNLFIFSNHYLYQVAHT
                     LFNVQPEQAYLVFKYAVVAQVPLAVIACWVLARDISGSLYTATMAAILIVFSPVFVLY
                     GGQVMTDVPSVLLLAVALIIHLRGIREKKFWMILLGAALLGFGVNLRETIAFYAPWLV
                     IAPFVLGWKFQRREVLWVAASCVVFVVLGLGWFGYWFITDPHYRWVWYGWRESMRDES
                     ARHPVQLSNLRPYVMLYFISAPLVFLPNLFAPVLEWRKGRISPMLLLWAVAFFANLLL
                     FFNYSTTVNWRYFLTGLPGIVPLSAFWLLRIAEKRVGSERRALFACSFLIALLAVIFF
                     IFMRPVSHEFIERRAMSKEYRHRLEEVPLDAIMISGAQTIAVNYWASIGSGRWKTIGT
                     GGGWPGDKLFSVVQNHLNHRRRVFIDADPRWWLPCGWQRDEIPLIVELGNRFSFRHVN
                     ETIYEISYLGDPAARDNPNLTKLLPENRPEDTKKCPPLKP"
     gene            13761..14948
                     /locus_tag="VFH96_07315"
     CDS             13761..14948
                     /locus_tag="VFH96_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973820.1"
                     /translation="MPYEVFLALRYLRSRRKRRLARATSVAAILGIAMGVAALIVAFA
                     LSNGFRDEMREKILQGTAHLSVVRADARPLENYSELASRVKQVDGVVTASATTYDGAL
                     VRGTKGSSYAVIRGVEKDAVHSSWLREGSFGPLFETSSNPSAVVGADLATRIGLSPGD
                     VFQVQPANDSTPRRLRVAGVFSSGLFEYDSTWIYVSMETASEFAGNNHAASVMSVQVN
                     DVDNVKQVSADVSKTLGNSYTIIDWQQANQPLFAALALERRMGLFIIGLIIAIATLNI
                     TTMLILVVVERRRDIAILSALGATRTGVMLLFIIEGAVVGAIGAAVGVAVGFVACAIG
                     NHYKLVSLPADVYSISNVPLIARPSEMLMAALVAFLLSVLATIYPARAASRMRPVEAL
                     RDS"
     gene            14945..15604
                     /locus_tag="VFH96_07320"
     CDS             14945..15604
                     /locus_tag="VFH96_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973821.1"
                     /translation="MISVVDLRKSFVAPNGERIEVLRGVTFTANSGESLAITGASGSG
                     KSTLLHLLGGLETPDHGNISVDGRRKEIGFVFQFHYLLPDLSAIENVALPLLIVRWKK
                     RLAFDRAMELLQETGLGDRAEHPIAHLSGGEQQRVAVARALITEPKLLLADEPTGNLD
                     EAISEQIGRTLIDYAHKHSAITIIATHSATLAGMCNRTLVLKAGRVLSTDYTDSFIES
                     V"
     gene            15698..>17005
                     /locus_tag="VFH96_07325"
     CDS             15698..>17005
                     /locus_tag="VFH96_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6973822.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGAPAIEPEHLLLGLMREDKTL
                     TGRFFPRAQVSIESIRKEIEGRTLLREKISTSVELPLAPETKHVLAYAHEESDRLQHR
                     HIGTEHLLLGLLREERSMAAEILYERGLRLNAVRDEIARQSGADARSTQKKDTPHLVE
                     FSRDLTDDAANDKLDPLIGREAEIERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIVRGEVPSFLEHKRILSLDLSLIVAGTKYRGQFEERLKQIMRELVENPQYIVFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEFRKSIEKDRSLERRFQAVKV
                     PPPSEAEAVSILDGVRERYESFHQVRYTDEALQAAVYQSHRYIPDRFLPDKAIDVIDE
                     AGARVKLRVRREQGNLADWNELNEWSRSYGDNTSFRMHQEDALV"
BASE COUNT         3581 a         4700 c         4757 g         3967 t
ORIGIN      
        1 tttcatcgtt taataggtcc gataagtcct atagcaccta tcgattcttg acacggctat
       61 ctccagcatg ttacaccgtc aaactcctga taagtgtagg tgttattaga aagcagccag
      121 agtcctcgcg cagtggcttt cggagaggtc atgcacaagc attgtgaccg tagagacgag
      181 cgagttaagt ttacccagtc gcattgatac gattgccacc gttgccgctg cggtcgctga
      241 ctttgttggc cgttcaggca tcagcgatga tgctgcgttt ggtatcgaca tggccgtgcg
      301 cgaggctgtc accaacgcag tgctccacgg caatcgtcag gatgaggaca aaaccgtaga
      361 cataactttg aagagctcgc ctgacgctgt tgaaatcagc gtgcacgacc agggcccagg
      421 tttcaacccg gaaacagtcc cagatccaac agcacaagaa aacattctta aagcctcagg
      481 ccgtggcatc tttttcatgc gcagttttat ggacgaagtc gactggttga tcagacccgg
      541 cggcggtacc acggtgcgca tgctgaagcg acgttaattc gcaattgcat aaaggagatt
      601 tctgaaatgg ctgagttgaa tatcaacgag cgtcaagccg gagatgtcac cgttcttgac
      661 ctgagcggaa agataaccat cggtgagggc agcgtggcgt tgcgcactgc tatcaggcga
      721 ttgctcgaag aaggcaagaa gcgcattctt ttgaaccttg ccggggtgag ctacgtcgat
      781 tccagcggta tcggcgagct tgtttccagc tacaccgcca tcggcaaaga acagggtgga
      841 cagctgaagt tactcaatct cacacagaaa attcgagact tactgacgat tacgaagctc
      901 ctgaccgtct tcgacgttta cgaaaacgaa gctgaagcac tcaacagcta caagtaacgg
      961 agaaagctaa tggctgagct cgatgtgaaa gaacgacaag cgggagatgt caccattctc
     1021 gatctgagtg gtgaagtgcg catcggcgag ggcgcggttt ccctgcgcga ttccattcgg
     1081 aacctctccg atcagggcaa aaagaaggtt ttgttgaatc tggcgggggt gaagtacatg
     1141 gattccagtg gtgtcggcga gttgatcgcc aactacacga ccgtgacccg ccagggcggg
     1201 cagctcaagt tgctgagctt aacggatcgg attcagaacc tgctggtgat tacgaagctg
     1261 ctgacagttt tcgattcttt cgataacgaa gcagaagctt tgaagagttt tgagtagctt
     1321 tcttctctgt gcaacctctg tgtcctctgt gtctctgtgg tgaaaggtca caacaaccac
     1381 agagacacag aggacacaga ggttgcacag agttatgttt cgtgccgcat ttcgccgtct
     1441 gcttgcgatc gacgatccac ccgagcggac ggcgcttgct ttttcaatcg gcgtctttat
     1501 cgccttctcg ccgttccttg gcctccacac catcatggct acggcgctcg cctttctgtt
     1561 tcgtttcaac aagatcgcga tctacaccgg aacctttgtc aacaacccgt ttctaacgct
     1621 cgttccgata attattgcgt cttacgctgt gggcgcattc atcattggcc gccctttgcg
     1681 catcccagac gagggcctgg agctcctgac acacccacgg atcttttccg gcgactatta
     1741 caagctgctt tttgtgcaga gttggaacca catcgtgtgg ccgttttcgg tcgggggtat
     1801 ggtgctctcg gtggtttgtt cggcactcgc ttatccgcta acattgcggg cgctgcgcgc
     1861 ttaccgagcc aggaaaaagc caaagattca cacggagtaa cacggattga agcatttttt
     1921 actgatcctc ctaattacag ttttgtctgc cggagtggca tcttcccaaa ccgttcaacg
     1981 agagccggct aaaccacaac catcaccgcc ggtccaacaa cgaccgcaga ccggtttcga
     2041 agtctctgaa tacggtgtcg attttcaggc cgatccgcgc ttgattctgg tgatggccgc
     2101 actcgacacc gccgggctcg atgcgacacc ggcgggccgg cagccttccg tttttcgcac
     2161 gaagctgcgg aaggatctcg ccaatctcga tcccgctctt cgcgagaaga tgaaaacttt
     2221 ttacgagcgc aatcgactgc ccgcgccggc gactccggca gaccaggccg cgcgctacgt
     2281 ttcactcgca ctggcgcttg gtccatcacc aaccttcgac gcgcccgagc gttccgaaga
     2341 tttgcccgcc ggacttttag aggtacttga tttcgcaccg ctcgtgcagg agttcgctcg
     2401 tcgatcaggt atcgacgaac acatggtcga gtacgtgcgc gcataccagg ctgaaggcga
     2461 ccggctgcgc gcgccggtgg ccgagatggt ccgtgggctt ctcacgtacc tgcacacacg
     2521 accgatcacg agctcggctg aacgagttga ggtcaaaaat ccaaacaaga agtcgaaaga
     2581 aaaggcgtac tcgttccggc aaaaagaacg ccgctttctg atcctgccgg acctgctggc
     2641 ggcgcgcggt gcgatcaatt tccgcatcat cggcgacgat tattacgccg ttgttccgga
     2701 aggaaccgat ccgagctcgt ctgagttgcg gcgtgcgtac ctgcagtatg ttatcgacgc
     2761 gctggtgctg cgattcaaca aagacatcgc gcttcgtcgc gatcagatca agcagctttt
     2821 gaacgaacga cagcaggctg gtgcgcaagt tactccggat gtgtttttga gtgtgagtcg
     2881 atcgctcgtg actgctgcgg atgcgcgtta cgaagagatg cgacggctcg aagttcttgg
     2941 gcgtgatgcg cgggcacaac tggcagccgc gaagaccgaa gcggaacgcg cgtcgatcgg
     3001 caagaccgcc acagaagaga tgaaagcgat ccaggacgag accgttgcgc gactcgccgc
     3061 agaatacgag aaaggcgcgg tgctctcgtt ttacttcgcc gatcagctaa agggaattga
     3121 gagtgccggg tttgacctcg caaacttttt cccagacatg atcgcgtcat ttgatccggc
     3181 gcgtgaagcc aagcgtccgt cggagtacgc cgaaacggtg cagagagcga cggcggcgcg
     3241 cgaggcccga acagccgcac gccgatcggc ttcggaagtc aacgtcgagg cgagtggcaa
     3301 agaagcggcg ctcgtgcgtg acctctcgtc gattgaagac acgctgcgca acaaggatta
     3361 caacgaagct gagaatcgct tgcgtgagat gttgaaggag tacccgcgcg agccgcgcat
     3421 ctttttcgcg ttggggcaaa cggcgagtct ggctgcatcg gatgcgactg atgagaacgt
     3481 gcaggctgag cgcctgaacc gcgcgttggg ccactatcgg atggctgtgg cagcctcgtc
     3541 accggaaact gacaaggcga tcatgtcacg cgcccacgag gcgatgggac gcatcaacat
     3601 gtttctcgaa aacactgcgg aagcggcgaa acagtttgat gaggcgataa agatcggcga
     3661 cgtgcgtggc ggtgcttaca gagaagcggt cgaagcaagg aagaagctaa atcagccttg
     3721 aatccgtgtt atccgcggct cattgaacgc ggataacacg gatgaaaacc ggatttagtt
     3781 cgcgagggct ttcgcggcgt tgattctgcc gccggttacg gtctttcctt tcagggcgac
     3841 gattggatcg gttgaggcga ggagcttctt tttcagatcg tcgactgaca tgcgaggatg
     3901 ctcggccagg atgagcgcgg ccacaccgga aaccaccggc gtcgccattg acgttcctga
     3961 cttctcctca taagcatccc cgagccatgt gctgaggata tctacgcccg gcgccgcgat
     4021 cgcgacactc ttcacacccc aattcgaaaa cctcgcgagc tcgtcgtggc gatcgagcgc
     4081 cgccacgctg accacgttcg gcacgttgta gctcgacggg taatgcggag tgcgatcgtt
     4141 atttaccgtc gaattgcctg ccgccgcgac aaacagaatg tcgttctcgt aggccttgcg
     4201 aatcgcatct tcgagcgcgc gcgagcgttg cgtcgaaccc cagcttgcac tgatgatgcg
     4261 aacgttcacg cccgccttct tccgatcgat cacgtaattg atcgcttcga tcgcgtcctt
     4321 cgtcgttcca aagccaccgg cgttcataaa cttcagcggc atgatcttca ctttccagtt
     4381 cacgcccgcg ataccgatgt tgttctcacc ttcggctccg atgatgccgg cgcagtgcgt
     4441 gccgtgacca ttctcgtcca tcggatcgga agcagtgtcg atcgcgttga aaccgttctc
     4501 gtcgtcgatc gtgccgagct cgttgtcgtg atacggcgcc atcgactcag gacgcatcca
     4561 catgttctgg ataagatctt cgtgcgtgta atcaacgcca ctgtcgagca ctgcaacgac
     4621 gactttatcg ctgccggtcg tggtggccca ggcgagcgtg gcgctgatgt ccgcgccctg
     4681 cttgccgccg cgctgtcctg aattcgcgag cgcccattga tcgttgaact gcggatcctg
     4741 cgggagaatc ggtacgagtg gtcctgaaga ctcagtcgcg tcaagctcga tctcgaaatt
     4801 cggctcggcg tactcgactt ccggcagttg ctggtattcg ttaacgacag tcgcagcatc
     4861 agcattgtcg agatcgtcaa tcgcttccag tccctcgacg ttctcgatgc ggtcttcgac
     4921 gcgatcgtga cgtagcgcgg tcagtcgctc gatggtttcc tggctaacgc cggttttgaa
     4981 tttgaccagc acttcgggac ggccgaagct gcgtgtttcg ctgagcgcga tctcttcagt
     5041 agtggttgct cttttcttct cagtggcggg aataggcccg atgtgcgacc tccactgttt
     5101 ccgccaccgg ctcaattgac cagcgaaagc ggcgacgaga acgagaacga gtgcgagcgc
     5161 gaggtggatg aagaaattat tgcgattcat ggtggtacct ctaaagcttt agatccggtg
     5221 atttttgccc ttaaagttaa aagccctgac ctggtttttg tcggcgcggt attcgtaggt
     5281 tttgccctgc gcttcgagct tgatgcgcca gccgctggtg atcatctggc cgctcatctc
     5341 gccgtcttcg gctgctccga gggccatgtc gggaaagtcg tcgtcttcga ctgacttttc
     5401 tttgatgtcg gcagcgttga cgttcaggcg cttggccagg tcgtttcgag cgttctcgac
     5461 tgcggaattt ttatcgatag ccatacggga ttcaaacgtc ccccctggat tctttgttct
     5521 cccattttga gcctgagatt cataatggtc aactcaaaac ggaaattttg ccgcggattt
     5581 tcgcggatga acgctgatca gaatccgcgt tcatctgcga aaatccgcgg ctaaactctt
     5641 acctgagatc tccaaaacga tgttgcatca ggacggtgac tgcgatcgct gccgtttccg
     5701 cgcgcaagac tctgccacca agcgtcacga tatgaaaatt tcgtgcccgt gctgcgtcga
     5761 tttcctcgtc agcccagcca ccttcggatc cgacgagggc cgttatcgag ctggctccag
     5821 ttaagctctc gaacgactcg ccaccgcgtt ccgagaacat tacgcctaca ccttcaacgt
     5881 ccaaagctgt ctcgaacggc accggcaaag cgatctccgg tacaaatgcc ctgccagact
     5941 gtttcaccgc ctcgagtgcg attcgctgcc agcgacccac gcgcttcgca gcgtcgcttt
     6001 gatcgcgtaa atgaatgtca gcatggcgcg ttatcagtgg cgttatcttc acgaccccaa
     6061 gctcagtcgt tttctgcacg acgagatcaa acttctcccc cttcaggagt gcgacgcaca
     6121 aatttagttg caactgtgat tcaggttttg cgggttgggc ttcggcgtcg attctcagtt
     6181 cggctgaatc gcgcttcgtg ttcaagacac tgcagcgaaa ttcacgtccg cggccgtcga
     6241 atacgtaaac ctcatcgccg gctttgagtc gcagcacgtc acggaggtgt cgcgcttcgt
     6301 cggcagtaag cgtgacggtc gtttgcgtga aggcggttgg cggggcgtgg aatcttcggc
     6361 gagtcattga gctaaacaat cagcgctatc cattcgccgt cctgtgtgat ctcaaattcg
     6421 tcgatcccgc tttcatgaag actagcctgg accatctcga gctgcgtttc caaaatgccc
     6481 gagaggatca atttcccgca cgtcacgccg accagcgccg gcattatcga cgtgatcacg
     6541 tcggcagtca gattcgccac cacgagatca gcggaggccg tcgaatcatc tatggagcct
     6601 gcccagaagt tgatctgtac ctcattcaac tctgcattct cgcgcgcgat cgcgatcgcg
     6661 gtttcatcgt tgtcgcaggc ctcaatatgt gctgatggaa agagtttagc agcggcgatc
     6721 gcgaggatgc cggtgcccgt gccgacatca agaaaactgc cgccggcgaa gtgtttttga
     6781 atcgcgtcga gacagagacg ggtcgtctcg tgagtgccgg tgccgaaagc catcccaggc
     6841 tcgatgcgaa ttactaatcg atcgtggacg tcgctgatgt tggaccacgg cggtgcgatg
     6901 atgaagcgcc cgatctcgac tggctgccag ctttgcttcc actcttccag ccaatcgcga
     6961 tctgcaactt cgcgcagatt catgtcgcgc acggacgacg acggcagttc gtaaatgcgc
     7021 aacgcttcaa acaattcatt gcgaacacgc tcgcgattcg gtgtctcagc aaagtaagcg
     7081 gtcacgccat gatcggtagt ttcagttccg agcgcaccgg cttccatcaa cgcatactcg
     7141 acagcctcgt gcgccgcgcc atcgatctca acatcgagcg cgtaccatcc actcggcttt
     7201 tctttagcgc tagtcaattc ccactcaatc catattcatc cagcagttgt tccttgttgc
     7261 gccagtcttg ttcgactttc acaaacagct cgagatgcac gcggtgaccc agcagcgcct
     7321 cgatgtcttg gcgcgcgcga atgccgatgt ctttcaaacg gctggcgcct tttccgatga
     7381 tgatcttttt ctgcgtagtt ctctcgacca cgatgacgca gttgatgcgc gtgaagtctt
     7441 cacgaatttc ttcgaagcgc tcggtaacga cggcggtgac gtatggaatc tcgtcggcgg
     7501 tcgcgtgcaa gactttctca cgcacgatct ccgaaacaag cacgcgcagc gactggtccg
     7561 tcaactcatc ttccgaaaag atcggctcgc cttccggcag gtgcttcaag gcgctctcga
     7621 tcaaaacgtc agtcatctca tccttcagtg ctgagatggg aatgatctcg gcccattcgt
     7681 gctcattgcg ataccaatct ataagtggca agagcgccga cttctcttca agcttatcga
     7741 tcttgttgag cagcaggagt gcaggctttt cggagcgttt gatgagatcg aggacaaagc
     7801 gatcgccgtt acccgtggaa acagaaacat cacgaatcag gcagacgaga tcgacgccca
     7861 tcagcgcctc ctgaaccgcc gccatcatcc gtcgattcaa ctcgtagcca ggttgatgca
     7921 cgccgggtgt atcgacgagc acgatctgcc cttcaggctt cgtgattaca ccctgaatcc
     7981 tgaatctcgt cgtctgcggc ttatcagaaa cagcagcgat cttctgtcca acgagccggt
     8041 taagcagcgt tgacttgccc gcatttggcc gaccaattaa cgccacataa ccactatgag
     8101 tcataatccg tgctttcgat ccgtataatc cgcgttaact cttctctgca aatatctgga
     8161 ccaggtggac cagcgtctcg gttgacttct cgagtccgcg gcgcgagttg tactcgagct
     8221 tgccatgaaa attcccgccg cccgtgaaaa gattaggcgt cggcaacccc tcgaacgtca
     8281 accgcgatcc gtccgtccca ccgcgaatcg gtttcagaaa cggcttcagc cccgctcgct
     8341 tcgctgcttc catcgcgttg ttcgtcagct ccggatagtt cttcagcact tccttcatgt
     8401 tcttgtaatt ctcttctacg tcgatcgtga tcttcacttg cggaaacttg tccgaagttt
     8461 tcgctaccat gtcgcgcaac aatttctctt ttgcatcgag accactgata tcgaaatcac
     8521 gcaacaggat cttcaaggtc gacttctcga catcgatcac acccgcgtaa ggatgaataa
     8581 aaccgacgcg cccttcagtc gtttccggca aaatgtcgtg tggaacactg ttgagataat
     8641 ccgcgatcgc atacatcgca ttgaccatga tccccttcgc cgttcccgga tgcgtgctct
     8701 ttccctggaa cgtgaccgtc gcggtgcgcg cgctccacgt ctcatcgctg atctcgccga
     8761 gctcttcacc gtcaaccgtg taagcgaact tcgcaccgaa acctttaacg tcaaacttct
     8821 cgatgcctcc gccaacctct tcgtcgggcg tgaacgcgat cgcgagggtg ccgtgtttga
     8881 gctgaggatt ctgacgcagg atatcgatca tcgtcatgat ctctgcgcaa cctgatttgt
     8941 cgtccgagcc gagcagcgtg gtcccgtcag cggtgatgat gtcgtcgccg atcagtgttt
     9001 tgagcgctgg cgttttgtcg accatgatca cctgcgttgg atcctttggc aacgtgatgt
     9061 cgccgccctg gtaattcttg tgaatgatcg cattgacgtt ttcacctgag actgcgggcg
     9121 acgtgtccat atgcgcgatg aaaccgatga ccggcacttt cgagttgtca gcgagattac
     9181 ccggaacgtt tgcgtaaacg atgccaaact cactaacgcg cacattctgt gctccgagcg
     9241 cggtcagctc cttcgcgagc aggttcgcga ggttgagctg tttgcgcgtg cttggtacgg
     9301 tcgtctggtc ttcctgcgat tgcgtgtcga tcttcacgta acgaagaaag cgatccatgg
     9361 cggattccac cggcggtttg agctgcgtgt tcgcgacgcc cacgcacacc agacaaagca
     9421 cgaatgcaat aatcgatttc attagttaac ctctcatagt gattttcgcc actcttcgct
     9481 ctgatgattc aaatcgtcga gagtttgctg caattcattg cgtttggccg ccagtgtcgc
     9541 ctcgtctgca tctgccggga cgtagatggg cggtgcaacg tagacgcggg cgagtgtgaa
     9601 cggcttcggg atctggaaga gatcccagct cggcgtcgtc caataacgag cgagcgccat
     9661 cgtgaccggc acgatcggct gacctgactt tttcgccagc agcaccgcgc ccatcttcgc
     9721 aatgtggcgc ggacccttcg gaccgtcgat cgtaaacgcc gtcgtgcatc cggcgcgcat
     9781 cagacgcgcc atctctatga tcgcgccgac accgccgcgc gtgctcgaac cgcgcaccgc
     9841 tccgtatcca aaccgctgaa taaaccgcgc aatatactca ccgtcaaaac tgcgcgacgt
     9901 catgacaacg atgcgtcgtt tgcgccacca gtaagtcgtc agaaaaatgc ggtcgtgcca
     9961 gaaaacgtag atcggaagac cgccattctc ttcggccttg tcgtgattct cccagccgat
    10021 gacttcaaag cgcgccgtca gcccgatcag atttatcagc aggtaaaata ccaaatcagc
    10081 cgcacgaatc ttcactcgat ccagaaacga atagctcgag aggtcggcga attcatacgc
    10141 gcgatcaact cgattaatcc gtgtattcat ccgtgtgcat ccgtggctaa taaatcttag
    10201 gctcgccggg cgggcgcgtc ttccagcgtt tgtgaatcca cagccactgc ccgggaaact
    10261 gcctgatctg gttctcgaga cgccgtgtga tctcgatcgt cacgcgccgg acgtcggcct
    10321 cttcatcgcc agtgcattca gccggaatcg gcggttcggc gattaataaa tactttcctt
    10381 tctcctcact ccacggcgcg aagaacggaa cgagcggcgt gttagtccgc actgcaagct
    10441 tcgcaatcac gaacgtcgta gccgcgggca caccaaaaaa atcgacaaag atcgcttcgt
    10501 catcgagcgt gttcagatcg ggcaaaagcc ccaatgcatg ttcaccggag cgcagcagct
    10561 ttaacatcga gcgcgccgcc gaaagtttgt cgatcgttct attgccaaac ttcgtacgga
    10621 cgccatcgac aagctcctcg atctttacgt tgtcgagcct gcgcgtcaga aacgtaaacg
    10681 ggtatccgag cagcgagata ccgaaactcg tcatctccca cgcgccgaga tgacccgtgt
    10741 agtagatcaa acggtcgcca cgctccgctc gcgcgttttc gagattctcc cagccaacag
    10801 gctcgatgag ttgcctgatc tcttcgcgag accgcgtcgc catctggctg aagagtccga
    10861 gctcacggcc gagactctcg aaacattcgc gcaacagcgc cgcccgctcc tcgtcagttt
    10921 tctccggaaa cgcgagacgc agattgatcg cgcccgtgcg acggagattg ccggccaata
    10981 ggtaaccgat tttgcctaac gaacggccga taccgtatga catgcggggc ggcaggcggc
    11041 caagtgtcat gaggacgagc ttgacgggcg cgtattccag ataggtgcga agctttccgt
    11101 gtttagccat tagctggaca ggattacagg atttacatga ttatcaggat tggtgcaaac
    11161 ccggtctgtc ctgtaacccg gccatcggtt tctgatatat ttcgaacgcg aggcgataga
    11221 ttttcgtaca ggccatcgaa gtttctctcc tcaaacgatt ctttcaagat tggagcttga
    11281 tggaaaaacg ccggatcctc atcgtcgata ataacgatga gttgcgtgtt ctactcgaac
    11341 aggctctcgg gcgcctgggt cacgaagtga tcgtgaccgg cgaacgtgag aaggcgctcc
    11401 gtcgtgacga tctcgatagt ttcgatctca tcatcagcga cctctcggaa gctgaagtaa
    11461 acggcgaacc catcggcgag ctgcaccgca gacacctcgt aaccgagctc aacggacaga
    11521 agaacggccc gggcgtgatc aaagcgttca aaatgggcgc cgcgaattac ctccgcgtgc
    11581 cttacaaccg tgatgagctg cgcgaaatag tagaacagac actcgccaat aaactccgtt
    11641 actgtgacga caccagcgct ttttcccacg tacacgaaaa gatcgagttt gagcttccca
    11701 gcgatctcgc gttgatgaat ggcgtgctcg actttctaca ggaacgcgtc gcgaagctcg
    11761 gtttgattcg tccggaacgc tcgaatcttt ttgttgcgct cgacgaggcg ttcgttaatg
    11821 cagttaagca cggcaataaa aacgatctga ccaaactcgt gaagatcact gccgagttgt
    11881 cgccgcacga agcgagtttc actgtcgaag atgaaggcca gggcttcgac attcgtgaga
    11941 ttccgaatcc gtgcgatccc gagaacctgt ttcgaacctc cggccgtgga gtgctcctca
    12001 tctacaacat catggacgaa gttgagtaca atgcgcaggg taatcgcgta aagatggtta
    12061 agagaccgga tgctccggtt aaagctcaac tcgttgaccc aaatacccct gacgacaaac
    12121 acgtctgaga cgtttccatt catacaatcc agtgccctga gtggtactgc ctcattttgg
    12181 cgtgcgtggc gatggtggtt gcctcccgca gtcgtcgcgt tagcactcgt tctgtatttc
    12241 gtcgatccgt tcattggcga ctgggatggc atggactaca ccatgctctc actcgcgggc
    12301 tatccgtcgt cgatggcgct cggccgaaac ctcttcatct tcagcaatca ctatctctac
    12361 caggttgcgc acacgctttt taacgttcag ccggagcagg catatctggt tttcaagtat
    12421 gcggtggtgg cccaggtgcc tctcgccgtg attgcgtgtt gggtccttgc ccgcgacatc
    12481 agcggctcgc tctacacggc gacgatggcg gcgatcctga tcgtgttctc gcccgtgttt
    12541 gtgctttacg gcgggcaggt gatgactgac gtgccgtcgg tgcttctgct cgcggtggcg
    12601 ctaatcattc atctgcgtgg cattcgtgaa aagaagttct ggatgatttt gctgggcgcg
    12661 gcgctgctgg gttttggagt gaacctgcgt gagacgatcg cgttttatgc gccgtggctg
    12721 gtgatcgcgc cgtttgttct cgggtggaag tttcaacgtc gcgaagtttt gtgggtggcg
    12781 gcgtcgtgcg ttgttttcgt agtgcttggg ctgggatggt ttggttactg gttcatcacc
    12841 gatccgcatt accgctgggt ttggtatggc tggcgtgaat cgatgcgtga tgagtcggcc
    12901 cgacatccgg tgcagctcag caacctgcgg ccgtatgtga tgctttactt cattagcgcg
    12961 ccgctcgtgt ttttgccgaa cttgtttgcg ccagtgctgg agtggcgcaa aggacgaata
    13021 tcgccgatgt tgttgttgtg ggcggtggcg ttcttcgcga acctgctgct gtttttcaat
    13081 tacagcacga ctgttaactg gcgttacttt ttaaccgggc tgccggggat cgtgccgttg
    13141 agtgcgttct ggttgttacg aattgctgaa aagcgtgtag gaagcgagcg ccgggcattg
    13201 tttgcgtgct cgtttttgat tgcgttgctg gccgtgatct ttttcatttt tatgaggccc
    13261 gtgagtcatg agtttattga gcggcgcgcg atgagtaagg agtacaggca tcgattagaa
    13321 gaagtgccgc tcgatgcgat catgatctcg ggcgcgcaaa cgatcgcggt gaattactgg
    13381 gcgtcgatcg gttcgggtcg ttggaagacg attgggactg gcggcgggtg gccgggtgat
    13441 aagttgtttt cggttgttca gaatcatttg aatcaccggc ggcgcgtgtt tattgacgcg
    13501 gatccgcgtt ggtggttgcc atgtggatgg cagcgcgatg agataccgct gattgttgag
    13561 ctggggaatc gttttagttt ccggcatgtg aacgagacga tttacgagat cagttatctc
    13621 ggtgatccgg cggcgcgcga caatccgaat ttgacgaagt tgttgccgga gaacaggccg
    13681 gaagatacga agaagtgtcc acctctcaaa ccttagccac ggatttgacg gatttaaaaa
    13741 accggatttg aacggatcag atgccgtacg aagttttttt agcgctgcgc tacttgagat
    13801 cgcggcgtaa aagacgattg gcgcgggcga cttccgttgc tgcgatactc gggattgcga
    13861 tgggtgtggc ggcgctgatt gtggcgtttg cgctgtcgaa tgggtttcgc gatgagatgc
    13921 gcgagaagat attgcagggc actgcgcatc tcagcgtcgt tcgtgctgat gcgagaccgc
    13981 ttgagaatta ttcagagctt gcgagtcgcg tgaagcaggt tgatggcgtc gtcaccgctt
    14041 cagcgacgac ttatgacggc gcactcgtac gcggaacaaa aggatcgagt tacgcggtta
    14101 ttcgtggcgt ggagaaagac gcggtccatt caagctggtt gagggaaggt tcgtttggac
    14161 cgctgtttga aacgtcatcg aatccgagcg cggtggtggg cgcagatctc gcgacgcgga
    14221 tcgggctctc gcccggtgat gtttttcagg tgcagcccgc gaatgactca acgccgcgtc
    14281 gcttgcgtgt cgccggcgtg tttagttcgg gactcttcga atacgactcg acgtggatct
    14341 atgtctcgat ggaaaccgcg agcgagtttg ccggtaacaa tcatgcggcg tcggtgatga
    14401 gcgttcaggt aaatgacgtt gacaacgtga agcaggtcag cgccgacgtt tcaaaaacgc
    14461 tcggcaacag ctacacgatc atcgactggc aacaagcaaa ccagccgctc ttcgccgcac
    14521 tcgcgctcga acgtcgcatg ggcctgttca tcattggtct aatcatcgcg atcgccaccc
    14581 tcaacatcac cacgatgctg attctcgtcg tagtcgaacg acgccgagac atcgcaatac
    14641 tcagcgccct cggcgccacg cgtacgggag tgatgttgct ctttatcatc gaaggtgcag
    14701 ttgttggcgc gatcggtgcg gcagtcggag tcgcggtcgg atttgtggcg tgcgcgatcg
    14761 gaaatcatta caagcttgtc agcctgccgg cagatgttta ctcaatcagc aatgtgccgc
    14821 tgatcgcgag accgagtgag atgttgatgg ccgcgctggt tgcattcctt ttgagtgtgc
    14881 tggcaacgat ctatcccgcg cgtgcagcgt cgcgcatgcg accggtcgag gcgctgcgcg
    14941 actcatgatc agtgtcgtcg atctgcggaa gagtttcgtt gcgccgaatg gtgagcgcat
    15001 cgaagtactg cgtggcgtga ccttcacggc gaattcgggt gagagccttg caatcaccgg
    15061 cgcttccgga tcgggaaagt cgacgttgct tcatttgttg ggtgggttag agacgccgga
    15121 ccacggcaac atctcggtcg acggtcgtcg gaaagagatc gggtttgttt ttcagtttca
    15181 ctacttgtta cccgatcttt cggcgattga gaacgtcgcc ttaccgttgt tgattgtgcg
    15241 ttggaagaag cggctggcgt ttgaccgggc catggagttg ttgcaggaaa ctggtttggg
    15301 tgaccgggcc gagcacccga tcgcgcatct atcgggcggg gagcagcagc gcgtggcggt
    15361 ggcgagggcg ttaattactg agccaaagct cttgttagcg gatgagccga cgggtaatct
    15421 cgacgaggcg attagcgagc agatcgggcg cactcttatc gattacgcgc acaaacattc
    15481 agcgatcacg attatcgcga cgcacagcgc gactttggca gggatgtgca atcgaacttt
    15541 ggtgctcaaa gctggaaggg ttttatccac agattacaca gactcattta tagaatcggt
    15601 gtaatctgtg gatagctact gaaaacatcg cttcgttacc acttttatct aggttataat
    15661 gcggcgtcgg taattataag gacgttacaa atacaagatg ttcgaacgct atactgaaaa
    15721 agcgcgacgg gtgatctttt tcgcgcgcta cgaagcgtca cagttcgggg ccccggccat
    15781 cgagccggag catctgttgc tgggactaat gcgtgaagac aagaccctca cgggccgctt
    15841 tttcccgcgc gcacaagttt caatcgagtc aattcgcaag gaaatcgagg gtcgtacgct
    15901 cctgcgtgag aagatttcga cttccgtcga gctgccgctc gcgccggaaa ccaagcacgt
    15961 tctcgcttat gcacacgagg aaagcgaccg cctccaacat cgacacatcg gtacagaaca
    16021 tctcctgctc ggcctgcttc gcgaagagcg ttccatggcc gctgagattc tttacgagcg
    16081 cggcctgcga ctcaatgccg ttcgcgatga gattgcacga cagtccggag ccgatgcgcg
    16141 ctccactcaa aaaaaagaca caccccactt ggttgagttc tcccgcgatc tgaccgacga
    16201 cgctgcgaat gacaagctcg acccgttgat cggtcgtgaa gcggagatcg aacgagtcgt
    16261 acagattctc tgccgccgca cgaagaacaa tccggtgctg atcggcgagc ccggggtcgg
    16321 taagacagcg atcgtcgaag gtctcgcgca gcgaatcgtg cgcggcgaag ttccctcgtt
    16381 cctcgagcac aaacgaattc tctcactcga tctttcgttg atcgttgcgg gtacgaagta
    16441 tcgcggccag tttgaagaac gactcaaaca gatcatgcgc gagctcgtcg aaaacccgca
    16501 gtacatcgtt ttcatcgacg agctgcacac gctcgtcggc gccggttctg ccgaaggttc
    16561 tctcgacgcg gcaaacattc tgaagcccgc gttatcacgg ggcgagattc agtgcatcgg
    16621 cgccaccacg cccgccgagt ttcgcaagtc aatcgaaaag gatcgctcgc tcgaacgacg
    16681 cttccaggca gtgaaggttc cgcctccatc cgaagctgaa gccgtcagca ttctcgacgg
    16741 cgtgcgcgag cgttacgaga gttttcacca ggtccgttac accgacgaag cgctgcaagc
    16801 cgctgtttac cagtcgcatc gctacattcc agatcgtttc ctgcctgaca aagcaatcga
    16861 cgtgattgac gaagccgggg cgcgcgtgaa actgcgcgtg cgacgcgagc agggaaatct
    16921 cgctgactgg aacgagctga atgagtggtc gcgatcttac ggcgacaata caagtttccg
    16981 catgcatcag gaagatgcgc tcgtt
//
LOCUS       DASSFQ010000206        28954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_692126, whole genome shotgun sequence.
ACCESSION   DASSFQ010000206 DASSFQ010000000
VERSION     DASSFQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 28954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28954
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692126"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFH96_07330"
     CDS             complement(<1..1041)
                     /locus_tag="VFH96_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6973823.1"
                     /translation="MYFKQFYLGCLAHASYLIGSEGEAVVVDPQRDVDEYLAEADAQG
                     LKIKYVLETHLHADFVSGHQEIAARTGAQIVFGEKAGVEFEHRAVKEGEEIRVGEVVL
                     RFIETPGHTPEGICILVTAPGEPQKLLTGDTLFIGDVGRPDLAGGKGYTPQMMAEMMY
                     DSLHGKILKLPDDTEVYPAHGAGSMCGRNMSKETSSTIGEQRKFNYALKPMSKEEFVT
                     MMTADLPEAPSYFPKDAEINRSGARGLSELQPPQPLTPQEVEQLQDNVLLDVRSAADF
                     GAGHVPGSMNIGLGGQFAMWAGSLIPLNASIVIIADTGAQVDESVVRLARVGIENVKG
                     FLEGGVTSWRDAG"
     gene            complement(1065..3896)
                     /locus_tag="VFH96_07335"
     CDS             complement(1065..3896)
                     /locus_tag="VFH96_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6973824.1"
                     /translation="MKETFYPGPSRSHLAAFPPKERWDNWTELDSRAWPQRKERHYSI
                     VPTTCFNCESACGLLAYIDKVTGDVQKFEGNPEHPGSRGRNCAKGPATLNQVTDPDRI
                     LYPLKRAGKRGEGKWERTTWDEALDTLATRIRNAIVENRRHEVMYHVGRPGEDGFTER
                     ILAAWGIDGHNSHTNICSSGSREGYQLWMGLDRPSPDHANAKVILLISAHLESGHYFN
                     PHAQRVMDAKANGAKLIVFDTRLSNTATHADYWVSPYPGSEAAMLLAIANYLIQNDLY
                     NREFVERWWNWKEFLAAEENPATDFADFEARLKQIYREYTFEFAAQESGVEARVLEEI
                     ARLVATAGTRFSSHNWRSVASGVSGGWMVARCLFMLNALLGAIATEGGVFPNAWNKFV
                     PRPIYTPPHPKMWNEITWPREYPLSMNEMSFLLPHLLKDGRAKLDVYFTRVYNPVWTN
                     PDGFSWIEMLTDPDLVGCYAALTPTWNETAYYADYVLPMGVGSERHDLHSYETHDSQW
                     LGFRQPVMKAMRQRDGETITDTRKVNPGEVWEENEFWLELTWRIDPDGSLGIRQYAES
                     KKNPGLRLGVDEYYEWIFDNSVPGLPERAAEENLTPLEFMRRYGAFEVKEKIGRVYEE
                     LVPAEEIDDARKDRFGRVYTRAAKPAGPNIVPLPLPDGDEEGRRLVGIEMDGAIKRGF
                     PTPSGRLEFYSSTMNGWGWPELAIPVYFKSHVHKDNLEEDQTILISTFRLAVQIHTRS
                     ANAKWLDEIAHTNPLWIHPSHAAKLGVRTGDLVRVETEIGYYVVAAWVTEGIRPGIVA
                     CSHHMGRWKLTDEGQRQLMATVRLDHNGSEWGLKREKGIEPYASEDPDTMRIWWKDAG
                     VHQNMTFPVHPDPISGMHCWHQAVRVRAAEADDKPGDIFVDTQKSREVYKKWLAMTRP
                     ADKFSPDGTRRPYWMLRPLKPAREFYNLR"
     gene            complement(3900..5447)
                     /locus_tag="VFH96_07340"
     CDS             complement(3900..5447)
                     /locus_tag="VFH96_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6973825.1"
                     /translation="MMERSDPIQWAKVIDHTRCIGCHACTTACKSENLVPLSVTRTYV
                     KHVDIGVFPQARRAHQVTRCNQCAHAPCVTACPTAAMFKRADGIVDFDKSICIGCKAC
                     MAACPYDAIFINPDDHSAEKCNFCAHRIDMKLEPACVVVCPTQAILVGDMNDPDSYVA
                     QIINRDTVAVRRPEKETLPKLFYKGAHQATLDPLAAKRPEGGLFMWSEQQEGAGYVTS
                     GNPNYSNSSAAALLSYDVAHSIPWDWRVSLYTWTKGIASGVYLIACLLVLLGVLNVNS
                     PLWLWATPIVSGGFLALTGALLLWDLEHPERFFMIFTRPQWRSWLVKGAFIIAGYTLV
                     LLLHFIASWVGSTTFQSWLMIAGIPLSLLTAIYTAYLFAQAKARDMWQNPLLPPHLVF
                     QAALLGSAILLPIATVMNNRVDLLLWILGVTSVIHLLMVWGEVSLTHPTAHARLAIWE
                     MVSGRYKSDFWMGTALSLIGGLLPLLAMLGIVSTQIGIGGAPFALIGLMLFENAYVQA
                     AQSVPLA"
     gene            5538..5867
                     /locus_tag="VFH96_07345"
     CDS             5538..5867
                     /locus_tag="VFH96_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6973826.1"
                     /translation="MRSTFYFALIVCAAAVLAACNAADHSANTNTKPAQLPGSDTVYA
                     DGARRVTADEMDTMVKNGQAIIIDVRNQASFDMGHIPGAKLIPAGEILNHLNELPRDK
                     MIVTYCS"
     gene            complement(6048..6899)
                     /locus_tag="VFH96_07350"
     CDS             complement(6048..6899)
                     /locus_tag="VFH96_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6973827.1"
                     /translation="MNNLRNLWITDCDRVGVVFPFRNILFPDDSVNAQAALKYANAFA
                     RHGHGQVVLFNVHDAKTELASKIAQAAIDQDIDLITVALRDRNLLTRAFGSSLGEDII
                     AESPCAVLAIRPPQRDFVNGQSELQLKRVLLATNFRPASVAATQLATQMANQLGAELH
                     AVYVIGDYFEQISSMFPEGGLAALTRLRSYVQERIANLSRGGGATHISEGRPYQEIVK
                     LADTLQADLIVIGTAVHGSLFGGNHVLGSEIERVIRNAPCPVLCVPAARVLTPLPALV
                     TQIVPQT"
     gene            7039..8769
                     /locus_tag="VFH96_07355"
     CDS             7039..8769
                     /locus_tag="VFH96_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6973828.1"
                     /translation="MLRGILRTKNLDEILAGVGGPQFGLKRTLTAFSVTLIGIGAIIG
                     AGIFATIGTASSGTAERAGAGPSLMLSFVITAIVCGFTALCYAELASMVPISGSAYTY
                     SYATLGELVAWIIGWDLIIEYAIGNVAVAISWAEFFNRLLRNLGIHMPDWLTSNYRTA
                     TKETLAAAPHIFGLPIVFNILAFGIVALITIVLVWGIKESSNFNAVMVLIKILVLLFF
                     IGLALYYVSPAKMAENWKPFQPQGWFGTLTGAAVVFFAYIGFDAVSTVAEETKNPARD
                     LPVGIIASLIICTILYVLVAGVFTGMVPWETVRAWTSAQRGESLTMALETVAPQALWQ
                     NTVIAFGSVVAHTAVLLVFQLGQPRIFFSMARDGLLPSVFASVHPRFKTPHVTTILTG
                     VAVGGIAAFATVDEMVSLTNIGTLFAFILVCVGVMVLRHKDPDRKRPFRVPFGPYLLP
                     GLGALSCALLIYFLPEGSYWRFVGWLMLGLSVYFSYGYTRSAIGQKLGRPSLTPVGLK
                     FAAVCFLLVAVGLFVIPHDVAPRELFSEAFDASAEKHNQTLYGLLIIIAGLIGAAVGI
                     ALGAPKEKEG"
     gene            8839..10683
                     /locus_tag="VFH96_07360"
     CDS             8839..10683
                     /locus_tag="VFH96_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET6973829.1"
                     /translation="MNALPIIIGSLCVIAISYRYYSAFIAAKVLALDDSRPVPSQTMF
                     DGHNYYPTNKWVLFGHHFAAISGAGPLIGPVLAAQFGFLPGLLWLVIGVCLGGAVHDF
                     MIMAGSVRRKGKSLAEMARTEISPLSGLVAGVAILFIVVIALAGLGLVVVNALAESPW
                     GTFTVAFTIPLALFMGLYMYRFRKGKIAEASIIGIIGLLFAVYLGGYIADSSLAQTFT
                     LSRNSLIVIMAAYGFIASVLPVWMLLCPRDYLSSYLKIGTIAFLIIGVILVHPNLNMP
                     AFTPFTAGGGPVIPGKLYPFVFITIACGAISGFHALISSGTTPKMIAKETDTRMIGYG
                     SMLMEGVVGIVALIAATSLFPGDYFAINTPQRTETQKAAYVQMVDTHTDQGFNLQPQE
                     IDRLEQESGEKDLRGRTGGAVTLALGIAKIFDGIPGLGGLMKYWYHFAIMFEALFILT
                     TIDTGTRVARFLVGEFGGRFYKKLEEPNWLPGSIVTSALVVTAWAAFIWSGSISTIWP
                     MFGIANQLLAAVALCVATTIIINSGRGKYAWVTILPLAFVSTTTLVAGFKSITDIFWP
                     LAQKPETSVQGLINTSLTVIIMVAAVIIMLDSIRRWMETRNYPQITQI"
     gene            10895..13411
                     /locus_tag="VFH96_07365"
     CDS             10895..13411
                     /locus_tag="VFH96_07365"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET6973830.1"
                     /translation="MELSLTLRRAAIIIAATVTTLALGIPTYAQSHQGGEASLELPDL
                     SQVTFLNGITGHNLLLFGIVICVLGLGFGLAIYMNLQKMPVHRAMREISELIYATCKT
                     YLTTQGKFILLLEAFIAVVIILYFGVLSGMEPFRVAIILLFSLIGIAGSYGVAWFGIR
                     VNTFANSRTAFASLEGKPFPLYAIPLKAGMSIGMMLISVELLIMLCILLFIPGSYAGP
                     CFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIVFGIKEDDARNPGVIADCTGDN
                     AGDSVGPSADGFETYGVTGVALITFILLGVTNPIVQVQLLVWIFVMRVMMIVASALSY
                     FINDAIAKARYKNADSMNFEAPLTSLVWITSVVSVVITFIVSYLTIPALSGNPTLWWK
                     LACIISCGTIAGALIPELVKIFTSTASRHVSEVVTSSREGGPSLNILSGLVAGNFSAY
                     WLGISIVGLMAIAYFFTSLGADAGLGLGALMVSPVAAPVFGFGLVAFGFLGMGPVTIA
                     VDSYGPVTDNAQSVYELSLIEQVPDVEAEVKKDFNIDINFSRAKHLLEENDGAGNTFK
                     ATAKPVLIGTAVVGATTMIFSIIMALTDGLRTDVESLSLLHAPFVLGLITGGAVIYWF
                     TGASTQAVTTGAYRAVEFIKANIRLEGVEKASIEDSKKVVQICTQYAQKGMFNIFMTV
                     FFSTLAFAFVEPFFFIGYLISIAIFGLYQAIFMANAGGAWDNAKKVVEVDLKMKGTPL
                     HDATVVGDTVGDPFKDTSSVAMNPVIKFTTLFGLLAVELAVSLTARNGNTTLTHILAL
                     VFFLVSVFFVWRSFHGMRIGTTAMETRPVEEEIVSRAAGD"
     gene            13415..13990
                     /locus_tag="VFH96_07370"
     CDS             13415..13990
                     /locus_tag="VFH96_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET6973831.1"
                     /translation="MSSYAKADTLMGAALAPLDSVRSAGLVIVFSLFIAACAQFSIHI
                     GPIPITGQTFAVLLTGMLLGSRLGAAAVIAYLIEGAIGLPFFAGGGAGLVRFLGPTAG
                     YLVAFPAAAFIVGAFAEHGWDKHYETAVAAMAIGSAIIFLGGWAWFAILTNTSPVAAF
                     QLAVLPFLLGDVIKIALGAAVLPTGWALLKK"
     gene            complement(14032..14289)
                     /locus_tag="VFH96_07375"
     CDS             complement(14032..14289)
                     /locus_tag="VFH96_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973832.1"
                     /translation="MAEDLDFQQARLAYSIIERLIEHNRVVSDLVAMMAQVLDEDTTK
                     ALTQTPTWTAYLDSRRALEKTRADVETFAEVLKELATDDTD"
     gene            complement(14282..15235)
                     /locus_tag="VFH96_07380"
     CDS             complement(14282..15235)
                     /locus_tag="VFH96_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET6973833.1"
                     /translation="MMTEYEVVRDGGAGLIDLSAVRGRIRVSGSEATMFLNGLITNDV
                     KNLAQNRWMPAAFPTVQGRLIGAVRVIRGSEPAFLIDTEMASHEAVLKTITRFTLAGD
                     FKVADVTGETALLTVQGQGATEVIGKVFNTGPSDLPDKGVAEMDGVTILRASHTGEEG
                     FDIVIDTSRKAEFQQSLEAAGAQPIGDDTFEILRVEAGIARFGQDMDETNVVPETNLD
                     EAVSYTKGCYVGQEIIVRIKHRGHPAKKLTGLRFETDQQIESGAVIRSTENQEIGRVT
                     SAVISPRQGSIGLGYVRYEYLAEGTRVVVGDGIEAIVGTNG"
     gene            complement(15257..15859)
                     /locus_tag="VFH96_07385"
     CDS             complement(15257..15859)
                     /locus_tag="VFH96_07385"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6973834.1"
                     /translation="MKNRVFKELVREAAGCTLCPAMCGRSAVLSELNGSPDARIMFIG
                     EAPGRKGADRTRVPFSGDQSGTNLDRFLSSINLTRKEIFITSAALCNPRSESGANRKP
                     TLRELSNCSGFLRRTIELVDPRVIVTLGSVALEALKRIQYHELSLKTAAAQIHSWNER
                     VLVPIYHPSPQVLASHRREAQQLIDYEVVAKAVTRINADL"
     gene            complement(15888..16376)
                     /locus_tag="VFH96_07390"
     CDS             complement(15888..16376)
                     /locus_tag="VFH96_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973835.1"
                     /translation="MNRLFLIILLVLVSVACSGNSDNAHTTATTIVPPPPRQVLRVEK
                     RAASQVPAQPDDPKLKVDAKKPLLVFNLPNGKTFREGEPVVLDFSLANAKLKGDGGEY
                     RVRYIVDDEETQWIDAHEQLVLTGWTLGKHTIRIELVGPDGWPYRNGDYNVVTRELTV
                     IK"
     gene            16449..17504
                     /locus_tag="VFH96_07395"
     CDS             16449..17504
                     /locus_tag="VFH96_07395"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET6973836.1"
                     /translation="MLKRKIAFLLLVIAFIGCGKNTVPISGTSSTSTSTSPKTQPLPA
                     GLAGYEAMRIPPDNPMTPEKVALGKQLFFDERLSIDGSRSCYSCHLCEHGLSDGLPKA
                     IGAENKPLTRNSPTLWNIGYHQAFYWDGRSDSLEKQAMAAWKGGNMGVGERTGEIVAK
                     INALKDYRSQFQKVFQSDATAENMMKAIAAFERTIIGGNTAFDRWRAGDQSAMSESAV
                     RGWNIFQGIKCNNCHDGILFTDQQFHNIGIGMDQENPDPGRYEVTKRREDTGAFKTPT
                     LRDIAKSAPYFHDGSAKTLEEAVDIMVAGGKPNEFLDKKNLQPRHPLPDQREALLDFL
                     RALTVDCTLSKPPLPQK"
     gene            complement(17534..18172)
                     /locus_tag="VFH96_07400"
     CDS             complement(17534..18172)
                     /locus_tag="VFH96_07400"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET6973837.1"
                     /translation="MSSTGSYQPRQFNLSGLKGISDQTLEMHFKLYEGYVKETNNLTN
                     RITEFIKDGNVDQEEMPAYSELTRRLGFEYNGMVLHEYYFDNLQSGGGTGDPSKTSQL
                     VKAAEATFGSYDIWKADFVGIGKMRGVGWAICNQNPANGRISNHWITLHETGNVAGFN
                     PILVMDVWEHAFLLDYKPAERPKYIEAFFSNVNWNAVEDRLKKIKGVQSARA"
     gene            complement(18331..19092)
                     /locus_tag="VFH96_07405"
     CDS             complement(18331..19092)
                     /locus_tag="VFH96_07405"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HET6973838.1"
                     /translation="MFQEVFHSPGGTDIARMILRWSHFVAGITWIGLLYFFNLVNVPF
                     QKGIDADTKKKVNPDLLGRALWYFRWGALITVLVGLTYYAMYILAVDVRNANTLGRGD
                     HVGILYILLFWLGYPLVLFAIEFLIIKKVPGVIKDGRVWAVVMFVLVAIFSYGLVRFF
                     ASAMTVGGESFASNKAYSIGIGGTYGIVMLLNVWGIIWPNNKRILAATAGIGPAAPPE
                     LARQAFIASRTNTWLSLPMLFFMATSHGDWIIFGR"
     gene            complement(19201..19536)
                     /locus_tag="VFH96_07410"
     CDS             complement(19201..19536)
                     /locus_tag="VFH96_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6973839.1"
                     /translation="MPKIQAETANGLVETEPPAGKKLVLALEDAGIDILHRCGGNARC
                     TTCRVEILEGEPGEMGELERNRLAVEAELAPNVRLSCQIHVNSDLKVRVINQASVRGM
                     DAGPRPLDE"
     gene            complement(19730..20086)
                     /locus_tag="VFH96_07415"
     CDS             complement(19730..20086)
                     /locus_tag="VFH96_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET6973840.1"
                     /translation="MSATTAPTVTLNVTESAATEIRKFMSSEEGLPETSGLRVRVVPG
                     GCSGFQYSLNIEEQSREGDFILDEKGVRMFVDMFSAQYLNGVEIDYVSGVMGSGFTFK
                     NPNATGSCGCGTSFTA"
     gene            complement(20151..21233)
                     /locus_tag="VFH96_07420"
     CDS             complement(20151..21233)
                     /locus_tag="VFH96_07420"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET6973841.1"
                     /translation="MNQLNEQLILDGLRQIKDPDLHKDIVTLGFIRDLKIDGGNVSFR
                     IVLTTPACPVKAEMESAAKEIVSSLPGVTSVAVTMDAEVPKGRGLGEKLVIEGVRNII
                     AVSSGKGGVGKSTVAVNLAVSLALDGARVGIMDADVYGPNVPIMLGASDARPEVDVNK
                     LIPIEAFGVKFMSMALLQPGDKPMIVRGPILHGLVKQFLSDVKWGELDYLIVDMPPGT
                     GDVQLSLAQLVPVQGAVLVTTPQNVAVADVRRALRMFETVAIPVLGIVENMSYFIAPD
                     TGTRYNIFGEGGGERLALTYHVPFLGAVPLGIEVREGGDNGVPVVVSKPDSPQAQAFR
                     KVTEEVARQVSIEAMKPELVVLGKGQ"
     gene            21300..21827
                     /locus_tag="VFH96_07425"
     CDS             21300..21827
                     /locus_tag="VFH96_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET6973842.1"
                     /translation="MTNGLDADHFFNPRNVGDADEPSFTGRAASLTCGAHLRFSIQVD
                     EGQRVSQARFRAAGCDVLIAAASMLTERVMGLTTAEAAGIGGESTSCTALARDALLDA
                     IREYSNAARDDWAGDEALICTCFFVSERTIEREICTHGLTTVAEVTRACNAGGGCGSC
                     HQLIQEILATNDLPR"
     gene            21811..22989
                     /gene="nifS"
                     /locus_tag="VFH96_07430"
     CDS             21811..22989
                     /gene="nifS"
                     /locus_tag="VFH96_07430"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HET6973843.1"
                     /translation="MIYLDNNATTRVAPEVLDAMKPYLSEFFGNPSSAHALGREMKRA
                     IEHSREQVVSLIGAAETSEIVFTSCGSESDNWAIRGVLATQPNKKHIITTQVEHEAVR
                     NVCRLLEQEGYEVTWLGVDGNGELDLDELRSALRPDTALVSIMLANNETGVLFPMEEI
                     GLIVREKSEAVFHVDGVQAVGKIPIDLKSTQADLFALSGHKLHAPQGIGALYIRKGLS
                     LPSFIIGGAQEQGRRAGTSAVPNIVGLGAACQLAVEDDNHELIERLRNRLEDEILANI
                     RNARLNGAADRSKRLPNTSNISFEYVDGQNILMHLDMEGICVSTGSACHSTTHESSPT
                     LRAMNVPFTAAQGSIRFSLGRYNTDADIDTTLRVLPSIIEKLAAMSPYPQIAQITQTT
                     "
     gene            23083..24945
                     /locus_tag="VFH96_07435"
     CDS             23083..24945
                     /locus_tag="VFH96_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET6973844.1"
                     /translation="MSTVIEPPVPQTEKNLEEVETVTIRFAGDSGDGMQLTGTQFTNT
                     SAVVGNDISTLPDFPAEIRAPAGSLPGVSGFQLNFSSQDIKTPGDQPNVLVAMNPAAL
                     KVNLADLEEGGTLIVNTDGFTAENLRYASYAANPLEDGSLAGYRVHKLPISTLNMNAL
                     KGNVELSRKEIDRSKNFFALGVLYWLYDRPMDNTLNWIRNKFAKSPELAKANEIALKT
                     GYNFADTTEVFTTHYTVKKAQIAPGKYRKITGNEATAIGFVAASQISGRPLFYGSYPI
                     TPASDILHELSRHKTFGVKTFQAEDEIAAVCAAIGASFAGQIGLTGTSGPGVALKQEA
                     IGLAVMTELPLVIVNVQRGGPSTGLPTKTEQADLFQSVWGRNGECPAIVVAPATPGDC
                     FHMAIEAVRLAFKYMSPVFYLSDGYLANGAEPWAIPAIDDLPKIDVSFANDPATFMPY
                     ARDEETLARPYALPGTPGLEHRVGGIEKQHITGNVNYDPENHHFMVKLRQEKVDRAAA
                     SIPLVEVMGESTGKVLVLGWGSTYGSISSAVEKMQREGKSVSAAHLRYLNPFPRNLGE
                     VLSGFETVIVPEMNLGQLCTMIRAKYLVDAVPFSKVKGRPFQIREIVRKVEEYL"
     gene            24945..25550
                     /locus_tag="VFH96_07440"
     CDS             24945..25550
                     /locus_tag="VFH96_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973845.1"
                     /translation="MLQPKASVIDVYEEARRYFMGEGTLNKTLARLSSDLEERGIDYM
                     VIGAVALLAHGYPRFTEDIDLVMTAEGLQKFHEELIGLGYTPAFPGARKRLRSTTDNV
                     SIEVMTTGEYPGDGKPKPVSMPEPSEASTEIDGIRFVNLEKLVELKLASGISAPDRLK
                     DLADVQELIKIKKLGADFALKLNPYVRAKYLELEEAVRRVN"
     gene            25564..26679
                     /locus_tag="VFH96_07445"
     CDS             25564..26679
                     /locus_tag="VFH96_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET6973846.1"
                     /translation="MSTQSNGHSGNGKPVTPVVSAESEAGSSVSGRVAPPPGQLKKAD
                     FISDQEVRWCPGCGDYAILNNVQKVMPELGIPREKIVFVSGIGCSSRFPYYMNTYGFH
                     SIHGRAPAIATGIKCANPDLSVWVITGDGDALSIGGNHFIHAIRRNLDLNYILFNNRI
                     YGLTKGQYSPTSEFGKKTKSTPAGTIDYPINPLSLAIASESTFVARSIDIDVKHLGTI
                     VEAAAKHKGISFVEVYQNCNIFNDHAFDYFAERTVRSDRVLYLEHGKPMVFGKNREKG
                     IRMNGAHPEVVTIGENGITENDLLVHDIHLNDPSVAFMLARMEQPAFPQPVGIFRAIE
                     RPTYEDMMVDQIEASVAKAGPGSLEKLINSGDTWIVE"
     gene            complement(26749..27390)
                     /locus_tag="VFH96_07450"
     CDS             complement(26749..27390)
                     /locus_tag="VFH96_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973847.1"
                     /translation="MNKALFVVVVICLVQLQALAQSEELPKFEVAAEFTTLEREAFSQ
                     RRTEPGFGGRLTYNLNKTVAFEGAGYFFPKRCFECRNAGNMTQGLAGVKVGKRFEKWG
                     VFAKARPGVISFSQGTFHIIPPIGLPFIPAQLELSRLTTFAMDVGGVVEFYPTKRIVT
                     RFDLGDTIIHFKRRNIDTILLDPVTNTFTVGTFPEPARTSHNFQFMTSVGFRF"
     gene            complement(27435..28520)
                     /locus_tag="VFH96_07455"
     CDS             complement(27435..28520)
                     /locus_tag="VFH96_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973848.1"
                     /translation="MRNKLWILLLLIHCLSVAVVGQNPAKRQLVMQVSNGGFVAFRSE
                     TPGAKSAPDSNSLAALLYSQAFAGNNRIIHRVLTDAANNVVFGYDLWINSDPISKKFS
                     LAVLPADEVFRRTFLKDYTPTRTNASFATFPKSTSPQTLDDGDAVSLDLLVNSESGVK
                     IVDVVSVTFDRATLREGYLESAPKDFTLDAVALSMKNYSLTINGNLVGKSKSSIGAEG
                     ALLWFYVPERGRFIFSLVPREGYNFAKVAVLDGNRIEFTVAGERYEWLSSASILPNGG
                     TWNLWVLHDADYTPMFGTTLIPPRSTQKGPNVFEKIEGALANRGAEVTFSLPGRSGSS
                     KSTTTAPQRVMVGGADSMENLLPKSAP"
     gene            complement(28510..>28954)
                     /locus_tag="VFH96_07460"
     CDS             complement(28510..>28954)
                     /locus_tag="VFH96_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973849.1"
                     /translation="TAPNPSHAEKQRRKEKLGPLFETMRLCVRTQVRIPLPLALAAII
                     ACLALGVFAFRRAEPAVAQPPLLVHVPVQVPVPQERVVTRVVYRDRRSSSRNSRRAIN
                     DAATESTFAKSRKPATDEIPASLNGFKPTDEIKLTVIKGGSPYEK"
BASE COUNT         6212 a         8181 c         7982 g         6579 t
ORIGIN      
        1 tccggcgtcg cgccagctcg tgacgccgcc ttcgagaaaa cccttcacgt tctcgatgcc
       61 gacgcgcgcg agtcgcacga ccgattcgtc aacctgcgcg ccagtgtcgg cgatgattac
      121 gatcgatgca ttcagcggaa tcaagctacc cgcccacatc gcaaactgcc cgccgagtcc
      181 gatgttcatc gagccgggaa cgtgaccggc gccgaagtct gccgcgctgc gcacatcgag
      241 caggacgttg tcctggagtt gctccacctc ttgcggtgtt aacggctgcg gtggttgcaa
      301 ctcggaaagt ccacgcgcac ccgagcgatt gatctctgca tctttcggaa agtagctggg
      361 cgcttcggga aggtctgcgg tcatcatcgt cacaaactct tctttactca tcggcttcaa
      421 cgcgtagttg aacttgcgtt gctcgccgat ggttgacgat gtttctttcg acatgttgcg
      481 accgcacatc gagccggcgc cgtgtgcggg atagacctcc gtatcgtcag gcagcttaag
      541 aatttttccg tgcaggctgt cgtacatcat ctcagccatc atttgcggtg tgtaaccctt
      601 gccgcctgcg agatcggggc gtccgacgtc gccgatgaag agcgtgtcgc cggttagtag
      661 cttttgcggt tcgccgggcg cagtaacgag aatgcaaatt ccttcaggcg tgtgacccgg
      721 cgtctcaata aaccgcaaga cgacctcacc caccctgatc tcttcaccct ctttcacagc
      781 ccgatgctca aactccacac cggctttttc gccaaagaca atttgagcgc cagtgcgcgc
      841 cgcgatctcc tgatgaccac tgacgaaatc agcgtgcagg tgcgtctcaa gcacatactt
      901 gatcttcaat ccctgcgcat cggcctccgc caaatactca tcaacatccc tctgcggatc
      961 gacaaccaca gcttcaccct ccgacccgat caggtacgaa gcatgcgcca aacacccaag
     1021 atagaattgc ttgaaataca taggtcttat tagtcctata ggttctatcg cagattataa
     1081 aactctcgcg ccggtttcaa cgggcgcaac atccaataag gtctgcgcgt cccgtccggc
     1141 gaaaacttat ccgccgggcg cgtcatcgcc agccattttt tataaacctc gcgcgacttc
     1201 tgcgtatcga caaaaatatc gcctggcttg tcatccgcct cagccgcccg cactcgcacc
     1261 gcctggtgcc agcaatgcat ccccgagatc ggatccggat gcaccggaaa agtcatgttc
     1321 tgatggacgc ccgcatcctt ccaccagatg cgcatcgtat ccggatcttc agacgcataa
     1381 ggctcgattc ctttctcacg cttcaagccc cattcgctgc cgttgtggtc caacctgacc
     1441 gtcgccatca actgccgctg cccctcatca gtcaacttcc aacggcccat gtgatggcta
     1501 catgcaacga tacccggtcg aattccttcc gtcacccacg ccgcgacgac gtagtaacca
     1561 atctcagttt caacacgcac gagatcgccg gtgcgcacgc cgagcttcgc cgcatggctg
     1621 ggatgtatcc agagcgggtt cgtgtgcgcg atctcgtcaa gccacttcgc attcgcgctc
     1681 cgggtatgga tttgcactgc gagccggaat gttgaaatca gaatagtctg atcttcctcg
     1741 agattgtctt tgtgaacgtg actcttgaaa taaaccggta tcgcgagctc cggccagccc
     1801 caaccattca ttgttgacga gtaaaactcg agcctcccgc tcggcgtcgg aaaaccgcgt
     1861 ttgattgctc cgtccatctc gatgccgacg agccggcgtc cctcttcatc accatcaggc
     1921 agcgggaggg gaacgatgtt tggtccagcc ggtttcgccg cgcgcgtgta gactcgccca
     1981 aaacgatctt tgcgcgcatc atcgatctct tccgccggaa ctagctcctc gtaaactcgc
     2041 ccgatcttct ctttcacttc gaacgcaccg tagcgacgca taaactccag cggcgtcaaa
     2101 ttctcctcgg cggcgcgttc cggcagacca ggaaccgagt tgtcgaaaat ccactcgtaa
     2161 tattcatcga cgccgagtcg caatcccgga ttcttcttcg actcggcata ctgccgaatc
     2221 ccgaggcttc catcaggatc gatccgccac gtcagctcga gccagaattc attctcttcc
     2281 cacacctcgc ccggattcac cttgcgcgtg tccgtgatcg tctcgccatc acgctgtcgc
     2341 atggctttca tcacgggctg gcggaaaccg agccactgcg aatcatgcgt ctcgtacgag
     2401 tgcagatcgt gtcgctccga tccgaccccc atcggcagca cgtagtcagc gtagtaagca
     2461 gtttcgttcc acgtcggcgt gagcgcggca taacaaccga cgagatccgg atcagtcaac
     2521 atctcgatcc acgagaaccc gtcagggttg gtccaaaccg ggttgtagac gcgcgtaaaa
     2581 taaacgtcga gcttcgcgcg tccgtctttc agcaagtgcg gcagcaggaa cgacatctca
     2641 ttcatcgaga ggggatactc gcgcggccac gtgatctcgt tccacatctt cggatgcggc
     2701 ggcgtgtaaa tcggccttgg cacaaacttg ttccacgcat ttggaaaaac accgccttca
     2761 gtggcaatcg cgcctagcag cgcattcaac atgaagagac aacgcgccac catccaaccg
     2821 ccactcacac ccgacgccac actgcgccag ttatgactcg aaaacctcgt ccccgccgta
     2881 gcaaccagcc tcgcaatctc ctcgagcacg cgcgcctcaa cccccgactc ctgcgccgca
     2941 aactcaaacg tatactcccg atagatctgt tttaatcgtg cttcaaaatc cgcaaaatcc
     3001 gtggccgggt tttcctctgc cgccagaaac tctttccaat tccaccacct ctctacaaac
     3061 tcacgattgt aaagatcgtt ctggatcaga taattcgcaa tcgccaacag catcgctgct
     3121 tccgatcccg gatatggcga tacccagtaa tcagcatgcg tcgcggtgtt cgaaagtcgc
     3181 gtgtcaaaca cgatcagctt cgcgccgttt gctttagcgt ccatcacccg ttgcgcgtgt
     3241 ggattgaagt aatggcccga ctcgagatgc gcgctgatca gcaaaatgac tttcgcattt
     3301 gcgtgatccg gactcggtcg atcaagaccc atccacagtt gatagccttc gcgactgcct
     3361 gacgagcaga tgtttgtgtg tgagttgtga ccgtcgatgc cccacgcggc aaggatgcgc
     3421 tcagtgaaac cgtcttcacc cggacgaccg acgtgataca tcacttcgtg acgccggttc
     3481 tcgacgattg cattgcgaat gcgggtggcg agtgtatcga gtgcttcgtc ccaggtcgtc
     3541 cgttcccatt ttccttcgcc gcgtttgcct gcccgtttga gtggataaag aatgcgatcg
     3601 ggatcggtta cctggttgag tgtggcggga cctttagcgc agttgcggcc acgcgagccc
     3661 gggtgttcgg gattgccttc aaacttctgc acatcgcccg tgactttgtc gatataagcg
     3721 agcaggccac acgccgattc gcagttgaaa cacgtagtcg ggacgatcga gtaatgacgt
     3781 tctttacgtt gcggccacgc gcgcgaatcg agctcggtcc agttgtccca gcgctccttc
     3841 ggtggaaacg cggcgagatg gctgcggctc gggcctgggt agaaagtctc tttcatcttt
     3901 tatgcgagtg gcacagactg cgctgcctga acgtaagcgt tctcgaagag catgagtccg
     3961 atcaaagcga acggtgcgcc gccgatgcct atttgtgtgc tcacaatgcc gagcatcgcg
     4021 agcaaaggca acagaccacc tatcagtgac agcgctgttc ccatccagaa gtcggatttg
     4081 taacgaccgc tcaccatttc ccagatcgcg agccgcgcgt gtgcggtcgg atgagttaac
     4141 gaaacttcac cccaaaccat caacaaatga atcacgctcg ttacgccgag gatccacaac
     4201 aacaagtcca cgcgattgtt catcacggtc gcgatcggca atagaatcgc cgaaccgagc
     4261 agcgccgcct gaaacacgag atgcggtggc agcagcgggt tctgccacat gtcgcgagct
     4321 tttgcctgcg cgaagagata cgcggtgtag attgccgtca gtaacgacaa cggtatgcct
     4381 gcaatcatca gccacgattg aaaggttgtg gatccaaccc agctcgcaat gaagtgcaac
     4441 agcagcacga gcgtgtagcc ggcgatgatg aacgcaccct tcacgagcca gctacgccat
     4501 tgtggccttg taaaaatcat gaagaaccgt tccggatgtt cgaggtccca gagcagcagc
     4561 gcgccggtga gcgccagaaa accaccggag acgatcggcg tggcccataa ccaaagcggg
     4621 ctgtttacgt tgagcacgcc cagcaacacg agcaaacacg caatcaggta aacaccggaa
     4681 gcgatgcctt tggtccaggt gtaaagactg acgcgccagt cccacgggat cgaatgcgcg
     4741 acgtcgtacg acagcaacgc cgccgccgat gagttgctgt aattaggatt acccgacgtg
     4801 acgtagcctg cgccttcctg ttgctcgctc cacatgaaga gtccgccttc aggacgtttc
     4861 gccgcgagcg gatcgagcgt cgcctggtgc gcgcctttgt aaaagagttt cggcagcgtc
     4921 tctttttcgg gacgcctgac ggccaccgta tctcgattga tgatctgtgc gacgtaagaa
     4981 tccgggtcgt tcatgtcgcc gacgagaatc gcctgcgtcg gacaaacaac aacgcacgcg
     5041 ggttcgagct tcatgtcgat gcgatgcgcg cagaagttac atttctcagc cgagtgatcg
     5101 tcgggattga tgaagatcgc gtcgtacggg cacgcagcca tgcacgcctt gcaaccgatg
     5161 cagatcgact tgtcaaaatc gacgatgccg tccgcgcgct tgaacatcgc cgccgtcggg
     5221 cacgcggtta cgcacggcgc gtgagcgcat tgattgcagc gggtgacctg gtgggcgcgg
     5281 cgcgcttgtg ggaagacgcc gatgtcgacg tgtttgacgt aggtgcgcgt gacggagagt
     5341 ggaacgagat tctcagactt gcacgcagtc gtacatgcgt gacagccgat acagcgcgtg
     5401 tggtcaatta ccttggccca ctgaatcggg tccgaccgtt ccatcataga agctcccgtt
     5461 gggaagcgag actataacat acgcgattga caaatcaggc gcggcgtaac acagtaacgg
     5521 gccttggagg tgtcttcatg cgttctacat tctattttgc attgattgtc tgcgcggcag
     5581 cggttttggc tgcgtgtaac gccgccgatc actcggctaa tactaatact aaacctgctc
     5641 agcttcccgg ttcggacacc gtctatgccg atggcgcgcg ccgtgtcacg gccgacgaga
     5701 tggacacgat ggtgaaaaac gggcaggcga ttattatcga cgtgcgcaac caggcctcat
     5761 ttgacatggg acacattccc ggcgccaagc tcatccccgc cggcgagatc ctgaaccacc
     5821 tgaacgagct tccgcgtgac aagatgattg tcacctactg ttcttgacag gccgaacata
     5881 cgtccgccgg tgcggtggtc gctctcaaga caaagggtta cgataacgcg gcagcgttgt
     5941 tgggtgggta caatctctgg caacaggaaa aacgacctat tgaaaagaag aattagccac
     6001 ggataacacg gataaaaatc cggatttgga tccgtgttat ccgtggccta ggtttgcggg
     6061 acgatctgcg tgacgagggc tggtaacggc gtcaggacgc gcgcggccgg cacgcagagc
     6121 accggacacg gcgcattcct gatcactctc tcaatctccg atcctagtac gtggttgcct
     6181 ccaaataacg atccgtgtac cgccgttccg atcacgatca aatccgcctg gagtgtgtcg
     6241 gcgagtttga cgatctcctg gtatggccgg ccttccgaga tgtgcgtcgc accgccccca
     6301 cgcgaaagat tagcgatgcg ctcctgcacg taactccgca atcgtgtcaa cgctgccagt
     6361 ccgccttcgg gaaacatcga tgagatctgt tcgaaatagt ctccaatcac ataaaccgca
     6421 tgcagctccg cgcctaactg attcgccatc tgtgtcgcta gctgcgtcgc cgcgactgac
     6481 gccggccgga agttcgtcgc gagcagcacg cgcttgagtt gaagctcgct ttgcccattg
     6541 acgaaatctc tttgcggcgg cctgatcgcc agcacagcgc acggcgactc cgcaatgatg
     6601 tcttcaccca gcgaactgcc gaacgcgcgt gtcagtagat tacgatcgcg cagtgccaca
     6661 gtaatcaaat cgatgtcttg atcgatcgca gcctgcgcga tctttgaagc aagctccgtt
     6721 tttgcatcgt gcacattgaa caacacgacc tgaccgtggc cgtgtcgcgc aaaagcattg
     6781 gcatacttca acgccgcctg cgcattcact gagtcatccg ggaagagaat gtttctgaac
     6841 ggaaacacga cgccaaccct atcacaatcc gttatccaca gattacgcag attgttcatt
     6901 gaatcggtgt aatctgagta atctgtggat aaataactct tccgtaacca tttacaacag
     6961 agtgacttcc atgccaaact ccgcaagctt tcctaacgca agcaacagtt atctaaatcc
     7021 actcccggag gcgacacctt gcttcgagga attttacgca ccaaaaacct tgacgaaatt
     7081 ctggccggcg tgggcgggcc ccagtttgga ctgaaacgaa cgctcactgc attcagcgtc
     7141 acgctcatcg ggattggagc catcatcggc gccggaattt tcgccactat cggcaccgcc
     7201 tcctcgggca ctgccgaacg cgcgggcgcc ggtccgtcgt tgatgctttc gtttgtcatt
     7261 acggcaattg tgtgcgggtt tacagcactc tgttacgccg agctggcctc gatggttccg
     7321 atctcaggct cggcttacac ctactcttac gcgacgctcg gcgagctcgt ggcgtggatt
     7381 atcggctggg acttgatcat cgagtatgcg attggaaacg tggcggtcgc gattagctgg
     7441 gccgagtttt tcaatcgact gctgcgcaac ctcggcatac acatgcctga ttggctcacg
     7501 tcaaactatc gcaccgcaac taaagagacg ctcgctgccg cgccgcacat cttcggcttg
     7561 ccgatagtct ttaacattct ggcgtttggc atcgtggcgc tgatcacgat cgtgctcgtg
     7621 tggggcatca aggagtcgtc aaacttcaac gccgtcatgg tgctgatcaa gatcctcgtg
     7681 ttgctgttct ttatcggcct cgcgctctac tacgtttcgc ccgcgaagat ggccgaaaac
     7741 tggaagccgt ttcaaccgca gggatggttt ggaacgctga ccggcgcggc ggtcgtgttt
     7801 ttcgcctaca tcggttttga cgcagtcagt actgtagctg aagaaacaaa gaatcccgcc
     7861 cgagatctgc ctgtcggaat catcgcctcg ctgatcattt gcacgattct ttatgtgctg
     7921 gttgcgggcg tgtttaccgg catggtgcca tgggaaacgg tgcgagcctg gacctccgcg
     7981 cagcgtggtg agtcgttgac gatggcgctc gagactgtag cgccacaggc gttgtggcag
     8041 aacacggtga tcgcgttcgg atcggtggtc gcgcacaccg ccgtgttgct ggtgtttcaa
     8101 ctcggacagc cgcgcatctt cttttcgatg gcccgcgatg gattgctgcc ttcggtgttc
     8161 gccagtgttc acccgcgctt taagacaccg cacgtcacaa cgatcctgac cggcgttgct
     8221 gtcggcggca ttgctgcgtt tgcgactgtg gacgagatgg tgagcttgac caatatcggc
     8281 actctcttcg cgttcattct cgtctgtgtc ggcgtgatgg ttttgcgtca caaagatccc
     8341 gatcggaaga gaccattcag ggtgccgttt ggtccttacc tgctgccggg tttgggcgcg
     8401 ttgtcatgcg cgctactgat ctacttcctg ccggaaggtt cctactggcg ctttgtcggt
     8461 tggctgatgc tcgggctgtc agtctacttt tcctatggat atacacgtag cgccatcggt
     8521 cagaagctcg gacgcccatc gctgactccc gtggggctga agtttgcagc cgtctgcttc
     8581 cttctcgtag cagtcggact cttcgtcatc ccgcacgacg ttgcgccgcg tgaattattc
     8641 tcggaagctt ttgacgcgtc cgcggagaag cacaatcaaa ccctctacgg tctactgata
     8701 atcatcgccg gcctgatcgg cgctgcagtt ggaattgccc tcggggcgcc caaagagaag
     8761 gaaggttaac cacagatgac acggataata aatccgattt tttaatccgt gttatccgtg
     8821 gttaggtttt agttttagat gaacgcgctt ccaatcatta tcggttcact ttgcgtgatt
     8881 gcgatctcgt atcgctacta ctcggcgttt attgcggcga aggtgctggc gctggatgac
     8941 tcgagaccgg tgccgtcgca gacgatgttt gatggacaca attattaccc gactaacaag
     9001 tgggttttgt ttggacatca cttcgccgcg atctcggggg cggggccgtt gattgggccg
     9061 gtgctggcgg cgcagtttgg atttttgccg ggactgttgt ggctcgtgat cggcgtgtgt
     9121 ctcggtggcg ccgtgcacga cttcatgatc atggccggca gtgtgcgacg gaaaggaaag
     9181 tcgctggcgg agatggcgcg caccgagatc agtccgttgt cgggattagt cgcgggtgtc
     9241 gcgatacttt ttatcgtcgt gatcgcgctc gcgggtcttg gtttggtcgt cgtgaatgcg
     9301 ctcgccgaat caccgtgggg aactttcacc gtcgccttta cgattccgct cgcactcttc
     9361 atgggtcttt acatgtatcg cttccgcaaa ggcaagattg ctgaggcgag catcatcggc
     9421 atcatcggac tgctgtttgc cgtctatctc ggtggctaca tcgccgattc ttcattagca
     9481 cagacgttca cactttcgcg caacagcctg atcgtgatca tggccgctta cggttttatc
     9541 gcgtcagtgt tgccggtgtg gatgctgctc tgcccgcgcg actacctgtc gtcttactta
     9601 aaaatcggca ccatcgcgtt tctgattatc ggcgtgatcc tcgtccatcc aaacctgaac
     9661 atgccggcgt tcacgccgtt cacagcaggt ggcggaccgg tgattcccgg caagctttat
     9721 ccatttgtct tcatcacgat cgcgtgtggt gcgatttccg gttttcacgc gctcatctct
     9781 tccggtacga cgccgaagat gatcgcgaaa gagactgata cgcggatgat cggttacggc
     9841 agcatgttga tggaaggcgt ggttgggatc gtggcgctta tcgccgcgac ttcgctcttt
     9901 cccggcgatt acttcgccat caacacgccg caacgcaccg agacgcaaaa ggctgcgtac
     9961 gtgcaaatgg tcgacacgca tacggaccag ggctttaatc ttcagccgca ggagatcgat
    10021 cgactcgaac aggaatcagg cgagaaagac ttgcggggtc gaaccggcgg cgcagtcacg
    10081 ctcgcgctcg gcattgcgaa gatcttcgat ggcataccgg gcctcggcgg gttaatgaag
    10141 tactggtatc acttcgcgat catgtttgaa gcgcttttca tcctcaccac gatcgacacg
    10201 ggcacgcgtg tggcgcgatt tctcgtgggg gaatttggcg ggcgtttcta caagaaactc
    10261 gaagagccaa actggctgcc gggatcgatc gtgacctcag cgctggtcgt cacagcgtgg
    10321 gcggcgttta tctggtcagg ctcgatctcg acgatctggc cgatgtttgg gattgcgaat
    10381 caactgcttg cggcggtggc tttgtgcgtg gcgacaacga tcatcatcaa ctccggtcgc
    10441 gggaaatatg cgtgggtgac gatcctgccg ctcgcgtttg tcagtaccac gacgctcgtc
    10501 gcgggcttta aatcgatcac cgacatcttc tggccgctcg cgcaaaaacc ggaaacgtcg
    10561 gttcagggcc ttatcaacac cagcctcacc gtaatcatca tggtcgcggc cgtgatcatc
    10621 atgctcgact cgatccggcg ctggatggaa acgcgcaact atccacagat tacacagatt
    10681 taagaggcag tctcataaac ctctgcggcc ctctgcgtat ctctgcgctc tctgcgttaa
    10741 acggtctatt tcaacgcaga ggccgcagag atacgcagag ggccgcagag aaatccaagg
    10801 ctctctttga aaaatctgtg taatctgtgg ataatacccg ttttcgtaaa gcgtatctcg
    10861 ccataatcta gacaaatcga aaggaatttt cgaagtggaa ctatcgctga cactgcgccg
    10921 tgcagcaatt atcattgcgg ccaccgttac gaccctggct ctcggcatcc caacttacgc
    10981 ccaatcccat cagggcggcg aagctagcct ggagctgccg gatctttcgc aggtgacctt
    11041 cctgaatggc atcaccggcc ataatttgct gctgtttggc attgttatct gcgttctcgg
    11101 gctcggtttc gggctcgcga tttacatgaa cctccagaaa atgccggtgc accgcgcaat
    11161 gcgcgagatt tccgagctga tctacgccac ctgcaaaact tacctgacca cacagggcaa
    11221 attcattctc cttctcgaag ccttcatcgc cgtcgtaatc attctttact tcggcgtgct
    11281 atcagggatg gagccgttcc gcgtcgcgat catcctgttg ttcagcttga tcggtatcgc
    11341 gggcagctac ggcgtcgcct ggtttggcat tcgcgtcaac actttcgcta actcgcgcac
    11401 ggctttcgcc agtcttgaag gcaaaccgtt cccgctttac gcaattccgc taaaagccgg
    11461 catgagcatc ggcatgatgc tgatcagcgt cgagctgctg atcatgctct gcattctgct
    11521 cttcattccg ggcagttacg cgggaccgtg tttcatcggt tttgcgatcg gcgagtcgct
    11581 gggcgcagcc gcactgcgta tcgcgggcgg catcttcacc aagattgccg acatcggttc
    11641 cgacctgatg aagatcgtct tcgggatcaa ggaagacgac gcgcgtaacc ccggcgtgat
    11701 tgcagactgc acgggcgaca acgcgggcga ctcagtcgga cccagcgccg acggttttga
    11761 aacttatggc gtaacgggcg tcgcgctaat caccttcatc ctgctcggcg ttaccaatcc
    11821 catcgtgcag gtgcagctac tggtttggat cttcgtcatg cgcgtgatga tgatcgtcgc
    11881 cagcgccctc tcctacttca tcaacgacgc aatcgcgaag gcgcgttaca agaatgctga
    11941 ttcgatgaac ttcgaagcgc cgttgacgtc gctcgtgtgg atcacgtcgg ttgtttccgt
    12001 agtgatcacg ttcattgttt cttacttgac gattccggcg ctcagcggca acccgacgct
    12061 ctggtggaaa ctcgcctgca ttatttcctg cggcacgatc gctggtgcac tgattcccga
    12121 gctcgtgaag atttttacgt cgaccgcgtc gcggcacgtc agcgaagtgg tgacgtcttc
    12181 gcgcgaaggc ggaccgtcgc tcaacattct ttccggtctc gtcgccggca atttctccgc
    12241 ctactggctc ggcatcagca tcgtcggcct gatggctatc gcatacttct tcactagtct
    12301 cggcgcagac gcgggattgg gcttaggtgc gttgatggtt tctccggtgg cggcgcctgt
    12361 gtttggtttc ggactcgtgg cgttcggctt cctcggtatg ggaccggtga cgatcgcggt
    12421 cgactcgtat ggtccagtga ccgacaacgc gcagtctgtt tacgagcttt cgttgatcga
    12481 gcaagtgccg gacgtcgaag cggaagtcaa aaaagacttc aacatcgata tcaacttctc
    12541 gcgcgccaag catcttctcg aagagaacga cggcgcgggc aacacgttca aagcgacagc
    12601 gaaaccggtg ctgatcggaa cggctgtggt cggtgcgacc acgatgatct tctcgatcat
    12661 catggcgctg accgatggac tgcgtaccga cgttgaaagc ctgtcgctgc tgcacgctcc
    12721 gttcgtgctc ggactgatca cgggcggcgc ggtgatctat tggttcacgg gcgcatcgac
    12781 tcaggccgtg accactggcg cttaccgcgc agttgagttc atcaaggcaa acatccggct
    12841 tgaaggtgtt gagaaagcct cgatcgaaga cagtaagaaa gtggtccaga tctgcacgca
    12901 gtatgcgcag aagggcatgt tcaatatctt catgacggtg ttcttctcga cgctcgcgtt
    12961 cgcgtttgtg gagccgttct tctttattgg ttacctgatt tcgatcgcga tctttggttt
    13021 gtaccaggcg atttttatgg cgaacgccgg cggcgcgtgg gacaatgcga agaaagttgt
    13081 cgaagtcgat ctgaagatga agggcacgcc gctgcacgat gcgacggtcg ttggcgacac
    13141 ggtcggcgat ccgttcaaag acacttcgtc cgtggcgatg aacccggtga tcaagtttac
    13201 gacgctgttc ggattgctgg cggttgagct ggcggtaagt ctgacggcgc gcaacggcaa
    13261 cacgacgttg acgcacatac tggcgctcgt gttcttcctc gtgtcagtat tcttcgtctg
    13321 gcgctcgttc cacggcatgc gcattggaac aacggcaatg gagacgcgtc cggttgaaga
    13381 agaaatcgtg agtcgtgcgg caggagacta attgatgagt agctacgcta aggctgacac
    13441 gttgatggga gcggcgctcg cgccgctcga ctccgttcgt tccgcggggc tggtgatcgt
    13501 tttcagtttg ttcatcgccg cgtgtgcaca gttttcgatt cacatcggtc cgataccgat
    13561 caccgggcag acatttgccg tgttgttgac ggggatgttg ctgggctcgc gtttgggcgc
    13621 agcggcggtc atcgcttacc tgatcgaagg cgcgattggc ttgccgttct ttgcgggcgg
    13681 tggcgcggga ctggtgcgat ttcttggtcc aactgcgggg tatctcgtcg catttccggc
    13741 ggcggcgttc atcgtgggcg cgtttgcaga gcatggttgg gacaagcact acgagacggc
    13801 ggtggcggcg atggcgatcg gctccgcgat catctttctc ggcggctggg cgtggtttgc
    13861 gattctcacg aacacgtcgc cggttgcggc gttccagctc gcggtgttgc cgtttctgct
    13921 gggtgatgtg atcaagatcg cgttgggtgc ggcagtgctg ccgactggtt gggcgttatt
    13981 gaagaaatga gccacggata aacacggaaa agacagatca aatccgattt tttaatccgt
    14041 gtcatccgtg gctaattctt tcagtacctc cgcgaacgtt tccacatccg ccctcgtctt
    14101 ttccaacgct ctccggctat cgagataagc agtccacgtc ggcgtttgcg tgagcgcctt
    14161 cgtcgtgtcc tcgtcgagca cctgggccat cattgctaca agatcactca ccacgcggtt
    14221 gtgctcgatc aaacgttcaa tgatcgaata cgccagccgc gcctgttgaa aatcaagatc
    14281 ttcagccatt cgttcctaca atcgcctcaa taccgtcacc aaccacgacg cgtgtgccct
    14341 cagcgagata ttcataacga acataaccga gcccgatgct tccctgtctc ggcgaaatca
    14401 ccgctgaagt gacgcgcccg atctcctgat tctccgtaga gcgaatcact gcgcccgatt
    14461 caatctgctg gtcagtctcg aaacgcaagc cagtcagttt tttcgccggg tgcccgcggt
    14521 gcttgatacg cacgatgatt tcctgtccga cgtaacagcc tttcgtgtaa ctcaccgcct
    14581 cgtcgagatt tgtctccggg acgacgttag tttcatccat gtcctgtccg aagcgtgcga
    14641 ttccggcctc gacgcgcaga atctcgaatg tgtcgtcacc aatcggttgt gcccctgctg
    14701 cttcaagaga ctgttgaaac tcagctttac gcgacgtgtc gatgacgatg tcgaaaccct
    14761 cttcaccagt gtgactcgca cgaaggatcg tcacaccgtc catctccgca acacctttgt
    14821 ccggcagatc cgaagggcct gtgttaaaga cctttccaat cacttccgtc gcgccttgcc
    14881 cctggaccgt caacaacgca gtctcacccg tcacatccgc gaccttaaaa tcacccgcga
    14941 gcgtgaaccg cgtgatcgtc ttcaacacag cctcgtgcga cgccatttca gtgtcgatca
    15001 aaaacgccgg ctcacttcca cgtatcacac gcacagcgcc gatcaatcgt ccctgcacag
    15061 tcggaaacgc cgcgggcatc caacggttct gcgcgaggtt cttcacatca ttcgtgatga
    15121 gcccgttcag aaacatcgtc gcctcggatc cactcacacg aatgcgccca cgcaccgccg
    15181 atagatcgat cagccccgcc ccaccatcac gtactacttc atattcagtc atcattctga
    15241 tccgtgttaa tcgtctttaa agatccgcat ttatccgtgt tacggctttt gctaccacct
    15301 cataatcgat cagttgctgc gcttcacgtc gatgcgacgc cagcacctgc gggctcggat
    15361 gataaatcgg cacgagcacg cgctcattcc atgagtgtat ttgcgcagcc gccgtcttca
    15421 agctcaattc gtgatattga atgcgcttta gtgcttcgag tgcaactgaa cccagcgtca
    15481 caatcacgcg cgggtcgacg agctcgatcg ttcgtcgcaa aaaccccgag cagttcgaca
    15541 actctcgtag cgtaggtttc cggtttgcgc ctgattcaga gcgtggatta cacagcgccg
    15601 cgctcgtaat gaagatctct ttgcgcgtca gatttatcga gctcagaaaa cgatcgagat
    15661 tcgtgcccga ctgatcgccg gaaaacggca cgcgcgtgcg atcggcgccc tttcgtcccg
    15721 gcgcttcacc aataaacatg atgcgtgcat ccggcgatcc attgagttcg ctcaataccg
    15781 cactgcggcc acacatcgcc gggcagagcg tacagcccgc ggcctccctc accaattcct
    15841 taaatactct attcttcatc cgtcttttat ccgtgaaaat ctgtggctca cttgatcacc
    15901 gtcagctcgc gcgtcacgac attgtaatca ccgttgcgat acggccaacc gtctggccca
    15961 acgagctcga tgcgaatcgt gtgtttgcca agcgtccagc cggtcagcac gagttgctcg
    16021 tgagcgtcga tccactgcgt ctcctcatca tcgacgatat aacgcacgcg atactcgcca
    16081 ccgtcgcctt tcaacttcgc gttcgcaagc gagaagtcga gcacaaccgg ctcgccttca
    16141 cgaaacgtct tgccgtttgg aaggttgaaa accagcaacg gcttcttcgc atccactttc
    16201 aatttgggat catcaggctg cgccgggacc tggcttgccg cgcgcttctc gacacgcaac
    16261 acctgtcgcg gcggtggggg aacgattgtc gtcgctgtcg tgtgcgcgtt gtcgctgttg
    16321 cccgaacacg cgaccgacac gagcactaac aggatgataa ggaaaagccg gttcataaat
    16381 tgtttatcca cagattacgc agattgttta taacatctgt gtaatctgtg gacgaagggg
    16441 cctcatttat gctcaagaga aaaattgctt ttctactgct cgtcattgca ttcatcggtt
    16501 gcggtaagaa cacggtcccg atttccggca catcgtctac gtccacatca acctcgccaa
    16561 aaactcagcc gcttcccgcc ggcctcgcgg gttacgaagc gatgcggatt cctcccgaca
    16621 atcccatgac gcccgagaag gtagcgctcg gcaagcagtt gttctttgac gagcgactga
    16681 gcatcgacgg ctcgcggtcg tgttactcgt gtcacctctg cgagcatggt ctatccgatg
    16741 gtttaccaaa agcgatcggc gcagaaaaca aaccactgac acgcaacagt ccgacgcttt
    16801 ggaacatcgg ctatcaccag gcgttttact gggacggccg cagcgattcg ctcgagaagc
    16861 aggccatggc tgcatggaaa ggcggcaaca tgggtgttgg cgaaaggacg ggcgagatcg
    16921 tcgcgaagat aaatgcgtta aaggattatc gatcgcagtt tcagaaagtg tttcaaagcg
    16981 atgcgacggc tgagaacatg atgaaagcga tcgctgcgtt tgagcggacg atcatcggcg
    17041 gcaatacggc gttcgatcgc tggcgcgcag gcgatcagag cgcgatgagt gaatcggcgg
    17101 tgcgtggttg gaatatcttt caggggatta agtgcaacaa ctgtcacgat ggaatcttgt
    17161 ttacggacca gcagtttcac aacatcggca tcggcatgga ccaggagaat ccggatccgg
    17221 gacgttacga agtgacgaaa aggcgcgagg acacgggcgc gttcaagaca ccgacattga
    17281 gagacatcgc gaagtcagcg ccgtactttc atgacgggag tgcaaagacg cttgaagaag
    17341 cggtggatat tatggtggct ggtgggaaac cgaacgagtt tctcgacaag aagaacctgc
    17401 agccgcgtca cccgcttccc gatcaacgcg aggcgctgct ggatttcctg cgggcgttaa
    17461 cggtggattg caccttgagc aagccaccgc tcccgcagaa gtaagccaca gattacacgg
    17521 attaaaaacc cgattaggcc cgtgccgact ggacgccctt gatcttcttt aatcgatctt
    17581 caactgcgtt ccagttgacg ttcgagaaga aggcttcgat gtacttcgga cgctcggcag
    17641 gtttgtagtc gagcagaaat gcgtgctccc aaacgtccat cacgaggatc ggattgaacc
    17701 ccgcgacatt ccccgtctca tgcaacgtga tccaatggtt tgagattctt ccgttggcag
    17761 gattctggtt gcagatggcc caacccacac cgcgcatctt tccgataccg acaaagtcag
    17821 ccttccaaat atcataactg ccgaatgtcg cttccgcagc tttcacaagc tgtgacgtct
    17881 tagacggatc gcccgtgccg ccaccgctct gaagattgtc gaagtaatac tcgtgcagga
    17941 ccatgccgtt gtactcaaac ccaagccggc gcgtgagctc ggagtaagcg ggcatttctt
    18001 cctggtccac gtttccgtct ttgatgaatt cggtgattcg gtttgtcagg ttgtttgttt
    18061 ctttgacgta gccttcgtag agtttgaagt gcatttccag cgtttgatct gaaatccctt
    18121 taaggccgct gagattaaac tgccgcggtt gataagagcc tgttgaactc atagatacct
    18181 cccctattcg gttgtgtcga tgttggagaa tatcgcgcgg aggtgtgaca aatacgttgt
    18241 gaggctggtg atttagtgtg gcagtccaca gattacgcag attacgcaga cttgatgaaa
    18301 taatctgcgc aatctgtgga taaaaaggtg ttatctaccg aagattatcc agtcgccgtg
    18361 tgatgtggcc ataaagaaca gcattggcag tgacagccat gtgttcgtgc gcgaagcgat
    18421 gaacgcctgt cgcgcgagct cgggtggcgc ggccggaccg atgcctgcag tcgcagccag
    18481 gatgcgtttg ttgttgggcc agattatgcc ccagacattc aacaacatca cgatgccgta
    18541 ggtgccgccg ataccgatcg aataggcttt gttactcgca aagctctcgc cgccgacagt
    18601 cattgctgat gcgaaaaacc tgacgagtcc atagctaaaa attgccacga ggacaaacat
    18661 gacaaccgcc cacacgcgac catccttgat cacccctgga actttcttaa tgatcagaaa
    18721 ctcaatcgcg aagaggacga gcggatagcc gagccaaaag agcagaatat acaagatgcc
    18781 gacatggtcg ccacgcccga gcgtattcgc gttgcgtacg tccactgcga ggatgtacat
    18841 cgcgtaatag gtcagtccaa ctaaaaccgt gataagcgcg ccccaacgga agtaccagag
    18901 tgcgcgtccg agcagatccg gattgacctt tttcttggtg tccgcatcga ttcccttttg
    18961 gaacggcacg ttcaccagat tgaagaagta aaggagaccg atccaggtaa taccggctac
    19021 gaagtgggac cagcggagaa tcattcgcgc gatatcagtg ccgccgggcg aatgaaaaac
    19081 ttcctggaac atagccagac tgatggggaa atgagctgtt gactcaatta gcatacgtcc
    19141 tccgtgttga taacctggaa ttacgggcga agattacacc tagaccaacc tgccgcgcaa
    19201 tcactcatca agcggacgtg gtcccgcatc catgccgcgc acacttgcct ggttgattac
    19261 acgaactttc aaatcactat tgacgtgaat ctggcatgac agacggacgt tgggcgcgag
    19321 ctctgcttct acggcgaggc ggtttcgttc gagctcgccc atttcaccgg gctcgccttc
    19381 gagaatctca acgcgacaag tggtgcagcg cgcgttgccg ccgcagcggt gcagtatgtc
    19441 gatgcccgcg tcttcgaggg cgagtacgag ttttttcccg gcaggtggct cagtttcgac
    19501 gagaccattg gcagtttccg cttgaatttt cggcattgat aagttcactc ctgattgttt
    19561 aggttggacg acaagctcag acaggattta caagatttct caggatagac aagatgaaat
    19621 catgtaaatc atgaaaaatc tagtaaatca tgtctaaggc tttatcttta aacacaaaca
    19681 ggaggcggga tccctcccgt ctcctgtcga aaaatcactc gcggcaggct taggcggtaa
    19741 acgaagtacc gcacccgcaa ctgccggtgg cattcggatt cttaaatgtg aaaccggagc
    19801 ccatgacgcc gctcacgtaa tcgatctcga cgccattcag atactgtgcg ctgaacatat
    19861 cgacaaacat ccgcacgccc ttctcgtcga ggatgaaatc gccttcgcgc gattgctctt
    19921 caatgttcaa gctgtactga aaaccagagc accctccggg cacaactctc acgcggagcc
    19981 ctgaggtttc gggtaggccc tcttcgctgg acatgaactt cctgatctcg gttgcggccg
    20041 attcggtgac gttgagtgtc acggtaggcg ctgtagttgc tgacatcttt actaatcctc
    20101 tctaaattcg tcgttcgtct gagtgctttg attactgaga cgaccttatc ttattgccct
    20161 ttgcccaaaa cgacaagctc aggtttcatg gcttcgatcg acacctgtcg cgccacttct
    20221 tccgtcacct ttcggaacgc ctgcgcctgc ggtgaatcgg gtttgctgac gactacaggc
    20281 acgccgttgt ctccgccttc gcgcacctcg attccgagcg gaaccgcgcc gaggaaaggc
    20341 acgtgatacg tgagcgcgag tcgctcgccg ccgccttcgc cgaagatgtt gtagcgtgtg
    20401 ccggtgtcgg gagcgatgaa gtagctcatg ttttccacga taccgagaac ggggatcgcg
    20461 acagtttcaa acatccgcag cgcgcggcgc acgtctgcga ccgcgacgtt ctgcggcgtc
    20521 gtcactaata ctgcgccttg caccggaact aattgagcga gcgataactg cacgtcgccg
    20581 gttcccggtg gcatgtcgac tatcaggtag tcgagctcgc cccatttcac gtcagataaa
    20641 aactgcttca cgaggccgtg caaaatcgga ccacgcacga tcatcggttt gtcgccgggt
    20701 tggagcagcg ccatcgacat aaacttcacg ccgaaagctt cgatcggaat gagcttgttt
    20761 acgtcgactt ccgggcgcgc gtcgctggcg ccgagcatga tcggaacgtt tggaccgtag
    20821 acgtcggcgt ccatgatccc gacgcgcgcg ccgtcgagtg cgagtgaaac cgcgaggttg
    20881 acggcgaccg tcgatttgcc gacgccgcct ttgcctgatg aaacggcgat gatgttgcgg
    20941 acgccttcga tgaccagttt ctcgccgagg ccgcggcctt tcgggacttc ggcgtccatc
    21001 gtcacggcca cggaggttac gcccggaagc gagctgacga tctccttcgc ggcgctttcc
    21061 atttctgctt tcactgggca ggccggcgtg gtcaggacga tgcgaaacga gacgttaccg
    21121 ccgtcgatct tcaggtcgcg gatgaaaccg agcgtcacga tatctttgtg caaatccgga
    21181 tctttgattt gtcttaagcc gtcgagtatg agttgttcat ttagttgatt cataagcgaa
    21241 aaagcgtagc tgattggcat tctctgtgca actccgtatc ttagccacgg ataacacgga
    21301 tgacgaacgg attagacgcg gatcatttct tcaaccctcg aaatgttggt gacgcagacg
    21361 agccgagctt taccgggcgt gctgcttcgt tgacgtgtgg ggcacatttg cggttctcga
    21421 tccaggtgga tgaagggcag agagtttcgc aggcgaggtt cagggcagcg ggttgtgatg
    21481 tcctaattgc cgcggcgtcg atgttgaccg agcgggtcat gggactgacg acggcggaag
    21541 cagcggggat cggcggcgaa tctacgagct gcactgccct cgctcgtgat gcactgctgg
    21601 acgcgatccg cgagtatagt aatgctgcgc gcgatgactg ggcgggcgat gaagccttga
    21661 tttgtacctg cttcttcgtc tcggagcgaa cgatcgagcg cgaaatctgc acccacggcc
    21721 taacgacggt cgccgaagtc acgagagctt gcaatgcggg cggtggctgt ggctcgtgtc
    21781 accaattgat tcaggagatt cttgctacca atgatttacc tcgataacaa cgccacgacg
    21841 cgcgttgcac cggaagtgct cgacgcgatg aagccttatc tgagcgaatt tttcggcaat
    21901 ccttcatcag cgcatgcgct cggccgcgag atgaaacgcg ctattgaaca ctcgcgcgaa
    21961 caagttgtga gtttgatcgg tgcggctgag acgagcgaga tcgtgttcac gtcgtgtgga
    22021 tcggagtcag acaactgggc catccgcggc gtgctcgcaa cccagccaaa caagaagcac
    22081 atcattacga cgcaagtgga acacgaagct gtgcgaaacg tctgtcgcct gctcgagcag
    22141 gaaggttatg aagtgacgtg gctgggtgtt gatggcaacg gtgagctcga tctcgacgag
    22201 ttgcgctctg ctttacgtcc cgacacagcc ctcgtctcga tcatgctcgc gaataacgag
    22261 accggcgtgt tgtttccgat ggaagagatc ggactcattg tgcgtgagaa atccgaagct
    22321 gtgtttcacg tcgatggcgt gcaggcagtc ggcaagatcc cgattgattt gaaaagcacg
    22381 caggcagatc tttttgcgct atcaggtcac aaactgcacg cgccccaggg catcggcgcg
    22441 ctttacatcc ggaaaggatt gtcgttgccg tcgttcatca tcggcggtgc gcaggagcag
    22501 ggacggcgtg ccggcacgtc tgctgttcca aacatcgtcg gactgggcgc tgcgtgccag
    22561 ctcgcagttg aggacgacaa tcacgagctt atcgaacgcc tgcgcaatcg tctcgaagac
    22621 gagatcctcg caaacatccg gaacgcacgt ctcaacgggg cggcagatcg ctcaaaacgt
    22681 ctgccaaaca cgtcgaacat ctcgttcgaa tacgttgacg gacaaaacat tctcatgcac
    22741 ctggacatgg aaggcatatg cgtttccacc ggctccgcct gccattcaac cacgcacgaa
    22801 tcatcgccaa cactgcgcgc gatgaacgtc ccattcaccg ccgcccaggg ctcgattcgc
    22861 ttctccctcg gccgttacaa caccgacgcc gacatcgaca cgaccctccg cgttcttccc
    22921 tcgatcatcg agaaactcgc tgccatgtcg ccttatccac agattgcaca gattacacag
    22981 acaacataaa catctgcggt atctgtgtaa tctgtggata gatgtcctat actgtctagt
    23041 ggcgacgatc acttttctga aaggccttaa agacaattag agatgagcac tgttatagaa
    23101 cctcctgttc cgcaaactga gaaaaatctg gaagaagtcg agactgtaac catccgcttc
    23161 gccggtgatt ctggcgacgg catgcagtta accggcacgc agttcaccaa cacctcggca
    23221 gtggtcggca acgacatctc gaccttgcct gacttccccg cagagattcg cgctcctgcc
    23281 ggcagcttgc cgggcgtcag cggatttcag ttgaacttct cgagccagga catcaagacc
    23341 cccggcgatc agccgaatgt cctcgtggcg atgaacccgg cggcgctgaa agtgaacctg
    23401 gctgacctcg aagaaggcgg tacgctgatt gtgaataccg acggcttcac cgcagagaat
    23461 ttgagatacg ccagctacgc ggcgaacccg ttggaagacg gctcgctcgc gggttaccgc
    23521 gtgcacaagc tgccgatctc gacgctcaac atgaacgccc tcaaaggcaa cgtcgagctc
    23581 tcgcgtaaag agatcgatcg ctcgaaaaac ttcttcgcgc tcggcgtgct ctactggctc
    23641 tacgaccgcc cgatggacaa cacgctcaac tggattcgca ataagtttgc taagagtccg
    23701 gagctcgcga aagccaacga gatcgcgctg aagactggtt ataacttcgc cgacacgacc
    23761 gaagttttca cgacgcacta cacagtcaag aaggcgcaga tcgctccggg caagtaccgg
    23821 aagatcacgg gcaacgaagc gactgcgatc gggtttgttg cggcgtcgca gatttcgggt
    23881 cgcccgctgt tttacggttc gtatccgatc acgcctgcgt ctgacattct gcacgagctg
    23941 tcgcgtcaca agactttcgg cgtgaagacg tttcaggctg aagacgagat tgcagcggtt
    24001 tgcgctgcga tcggcgcgag cttcgcaggc cagattggat tgactggaac gtccggaccg
    24061 ggtgtggctt tgaaacagga agcgatcggt cttgctgtga tgactgagct gccactcgtg
    24121 atcgtcaacg ttcagcgtgg tggaccatcg accggcttgc cgacgaaaac tgaacaagct
    24181 gatttgttcc agtctgtctg gggacgaaat ggcgagtgtc cggcgatcgt cgtagcgcct
    24241 gcaacgccgg gtgactgttt ccacatggcg atcgaagccg tgcgactggc gttcaagtac
    24301 atgtcgccgg tgttttacct gtctgacggt tatctcgcga acggcgcgga gccgtgggcg
    24361 attcccgcga tcgacgatct gccgaagatt gatgtcagct tcgctaacga tccggcgacg
    24421 ttcatgcctt acgcgcgcga tgaggaaaca ctggcgcggc cctatgcgct gccgggcacg
    24481 ccgggcctcg agcatcgtgt cggcggtatc gagaaacagc acatcaccgg caacgtcaac
    24541 tacgatcccg agaaccacca tttcatggtg aagctgcgcc aggaaaaagt agaccgcgcc
    24601 gccgcgtcaa ttcccctcgt ggaagtgatg ggtgagtcga cggggaaagt cctcgtgctc
    24661 ggctggggct cgacttacgg ctcaatctcg tcggcagttg aaaagatgca gcgcgaagga
    24721 aagagtgttt cagcagcgca cctgcgctac ctgaatccct tcccacgcaa tctcggcgaa
    24781 gtattatcag gcttcgaaac cgtaatcgtc cccgagatga acctgggcca actctgcacg
    24841 atgatccgcg cgaagtatct ggtcgacgcg gtccccttct cgaaagtgaa aggccgcccg
    24901 ttccaaatcc gcgagatcgt caggaaggtc gaagagtatt tgtgatgttg cagccgaagg
    24961 catctgttat agatgtgtat gaggaagctc gtcgctactt tatgggtgaa ggtacgctca
    25021 ataaaactct cgcacgtctt tccagtgatc tcgaagagcg tggcatcgat tacatggtca
    25081 tcggagcggt cgctttgttg gcgcatggtt atccgcggtt cactgaagac atcgatttgg
    25141 tgatgactgc tgaaggtctt cagaaatttc acgaagaact catcgggctc ggctacacgc
    25201 cggcgtttcc aggcgcaaga aaacggctgc gatcaaccac ggacaacgtc agcattgaag
    25261 ttatgaccac cggagagtat ccgggcgacg gaaagcccaa gccggttagc atgccggagc
    25321 cgtcagaagc ttcgaccgaa atcgacggga tcagattcgt aaatctcgaa aaactggtcg
    25381 agttgaagct tgcgtcggga atttctgcgc ccgatcggtt gaaggatctg gcagacgttc
    25441 aagagttgat caagatcaaa aaactcggtg ccgattttgc gctcaaactc aatccgtatg
    25501 tgcgagcgaa atatctcgaa ctcgaagaag ctgtgagaag agtgaattaa ttcaggagaa
    25561 ataatgagta ctcagagtaa cggtcatagt gggaatggga agccggtgac gccggtggtt
    25621 tcggcggaga gtgaggcggg gtcgtcggtg tcggggcgtg tggcgccgcc gccgggacag
    25681 ttgaagaagg ctgattttat ttccgaccag gaagtgcgat ggtgtccggg atgtggtgat
    25741 tacgccattc tgaacaacgt gcagaaggtg atgccggagc tcggcattcc gcgcgagaag
    25801 attgtgtttg tgtcgggtat cggatgctcg tcgcgttttc cttactacat gaacacgtac
    25861 ggcttccaca gcattcacgg gcgcgcgccc gcgattgcaa ccggaatcaa gtgtgccaat
    25921 cccgatctca gtgtttgggt gatcacgggc gacggcgatg ccctctcgat cggcggcaat
    25981 cacttcattc acgcgattcg ccgcaatctc gatctgaatt acatcctttt caacaaccgc
    26041 atctacggtt tgacgaaggg acagtacagt ccgacttctg agtttggtaa gaagacgaag
    26101 tcgacgccgg cgggcaccat cgactatccg atcaatccgc tttcactcgc gatcgcgagc
    26161 gagtcgacat ttgtcgcgcg ctcgatcgat attgacgtca aacacctggg cacgatcgtt
    26221 gaagcggcgg cgaagcacaa gggcatctcg tttgtcgagg tctatcaaaa ctgcaacatc
    26281 ttcaacgatc acgcatttga ttacttcgct gagcgtacgg tgcgctcgga tcgcgtgctg
    26341 tacctcgaac acggtaagcc gatggttttc gggaagaacc gcgaaaaagg aattcgcatg
    26401 aatggcgcgc atccggaagt cgtgacgatt ggtgagaatg gcatcaccga gaacgatctg
    26461 ctggtgcatg acattcacct gaacgatccg tcagtggcgt ttatgctggc gcgcatggag
    26521 cagccggcat tcccgcagcc cgtgggtatc ttccgcgcga ttgagcgacc gacttacgaa
    26581 gacatgatgg tcgatcagat cgaggcatcc gtggcgaagg ccggaccggg cagtctcgag
    26641 aagttgatca atagcggcga cacctggatc gtcgagtaaa aacctggcca cagattacac
    26701 ggataaaaga tcggatttaa aatccgtgta atctgtggcc aaactctatt aaaagcgaaa
    26761 tccgacgctc gtcatgaatt gaaaattgtg tgacgtgcgt gcgggttccg ggaatgtccc
    26821 gaccgtgaat gtattcgtca ccggatccaa caggatcgtg tcgatattgc gacgcttgaa
    26881 atggatgatc gtgtcgccta agtcaaagcg cgtcacgatc cgctttgtgg gataaaactc
    26941 gactacgccg ccgacgtcca tcgcaaatgt tgttaaccgg ctgagctcga gttgggccgg
    27001 gatgaacggc aaaccgatag gcggaattat gtgaaacgtg ccctggctga agctaatcac
    27061 ccccggccgt gctttcgcga agacgcccca cttttcaaat cgcttaccga ctttcactcc
    27121 cgctaagcct tgcgtcatat tccccgcgtt gcgacactca aaacatcgct tcggaaagaa
    27181 gtagccggcg ccttcaaagg cgactgtttt gttgagattg taagttaagc gcccgccaaa
    27241 tccgggctct gttctgcgtt gtgaaaaggc ctcacgctcg agcgtggtga actccgctgc
    27301 aacctcaaac ttcggaagct cctccgactg ggccaatgct tgcaattgaa ctagacagat
    27361 cacgacaaca acaaagagag ctttgttcat tctgtgactc cttagagagt tgtgggaaga
    27421 cttagtggat aaatttaggg agcactttta ggtagaaggt tttccatcga gtcagcgcct
    27481 ccaaccatca cacgctgcgg tgcggtggtc gtgcttttgc tcgagcctga ccgcccgggc
    27541 aggctaaacg tgacttctgc gccacgattg gccaacgcgc cctcgatctt ttcaaacacg
    27601 tttggacctt tctgcgtcga gcgcggaggt atgagtgtgg taccaaacat cggcgtgtaa
    27661 tcggcgtcgt gcaacaccca aagattccac gtgccgccat tcggcagaat cgaggcgctc
    27721 gagagccact cgtatcgttc gccagcaacg gtaaactcga tccggtttcc atcaagcact
    27781 gcgaccttcg caaagttgta accctcgcgc ggcacgagtg agaaaataaa gcgaccgcgt
    27841 tcgggcacgt agaaccacag cagcgcacct tcggcgccga tgctcgattt acttttgccg
    27901 acgagattgc cgttgatcgt caagctgtag ttcttcatcg agagcgccac agcgtcgagc
    27961 gtgaaatctt tcggcgccga ctcgaggtaa ccttcgcgca gtgtggcacg atcgaaagta
    28021 acgctgacca cgtcaacaat cttcaccccc gattcactat tgacgagcag atcgagcgac
    28081 acggcatcgc cgtcatccag cgtttgcggc gatgttgatt tggggaaagt ggcaaatgaa
    28141 gcgttggttc gcgttggtgt gtagtctttc aaaaacgtgc gacgaaacac ttcatcagcg
    28201 ggcaaaacgg cgaggctgaa tttcttactg atcggatccg agttgatcca gaggtcgtaa
    28261 ccgaagacga cgttgttcgc cgcatcggtc agcacgcgat gaatgatgcg gttgtttccg
    28321 gcgaacgcct gcgagtaaag cagcgccgcg agcgaattgc tgtcgggcgc gctcttcgct
    28381 cccggtgttt cacttctaaa cgcgacgaag ccgccgttgg aaacctgcat cacgagctgg
    28441 cgctttgccg gattttgacc cactaccgcg acgcttaaac agtgaatgag tagcagaagg
    28501 atccagagct tatttctcat acggagatcc tcccttgata acggtgagct tgatctcgtc
    28561 tgttggtttg aagccgttga gacttgctgg aatctcgtcg gttgcgggct ttcgcgattt
    28621 ggcaaacgtg ctttcagttg ccgcgtcgtt gattgcacgt cgcgaatttc ttgacgatga
    28681 gcggcggtcg cggtaaacga cgcgcgtcac gactctttcc tggggcaccg gaacctggac
    28741 cggtacgtgt acgagcaacg gaggttgggc cactgctggt tcagcacggc gaaacgcgaa
    28801 tacacctaag gcgaggcagg caatgatcgc tgcgagggcg agcggtaacg gaatgcgaac
    28861 ctgtgttctc acgcagagtc gcatagtctc aaagagaggc ccgagttttt ctttgcgtct
    28921 ctgtttctct gcgtgagaag ggttgggagc ggtc
//
LOCUS       DASSFQ010000207         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_994102, whole genome shotgun sequence.
ACCESSION   DASSFQ010000207 DASSFQ010000000
VERSION     DASSFQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994102"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFH96_07465"
     CDS             <1..544
                     /locus_tag="VFH96_07465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6973850.1"
                     /translation="AALLFLAALAEEAMFRGYGLQTLTRAKLAWLGILLTSVPFGLVH
                     LNNPNVVPVVTVTNTVLAGIWLAAAYLRTRSLWLAVGVHWSWNWALGWFFGLPVSGMT
                     VVSNPLLEAQDIGPKWLTGGTYGIEGGIACTIALILFTAFLWKTRWLSATPELKQMTS
                     EENPATPEPVISIRPANDNA"
     gene            619..1191
                     /locus_tag="VFH96_07470"
     CDS             619..1191
                     /locus_tag="VFH96_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6973851.1"
                     /translation="MYATADMLLTSPQAHAEQSQEPAIEVVLVRAVLQGDREGFSRLY
                     DLYAPLVHGILLARVPRAEVADLVQDIFFHAFKKLHTLRDEAAFGPWIAMIARNRAVD
                     FHRRSKEMVEINDDLRGSDQHDSRAAEILELIRSLPEAYRETLVLRLVEGMTGPEIAA
                     RTGLTAASVRVNLHRGMKLLRERLGFMEGL"
     gene            1188..1676
                     /locus_tag="VFH96_07475"
     CDS             1188..1676
                     /locus_tag="VFH96_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973852.1"
                     /translation="MNDDYLWDKTGEPDPQIQQLEEILGTLRYQPRPLVLPRKRNYFP
                     LLAIAASLLLALLAGGIWLRLRSRNEVPPRQARIETPTPAPKRDVTPVLPEKKSAVNP
                     SPVVANNRRRHRSVPSAAEREEALMAKQQLMLALRLTTEKLHLVRTRTQPNQIKNQHR
                     VG"
     gene            1688..2272
                     /locus_tag="VFH96_07480"
     CDS             1688..2272
                     /locus_tag="VFH96_07480"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HET6973853.1"
                     /translation="MKTLFHINIRTALIAALLVLAPIFAAQAQQGSRIQMSALDHLAA
                     KASQTVDVNIDERLMRLAARVFDDKDEDEREIKKLIANLKGVYVKSFEFETDGQYVAA
                     DLEPIRTQLRGPGWTRLVNVTSKKEGMLEVYLLFNGDVVGGLAVLHTDDKELTVVNIV
                     GPVDVDKLARLEGQLGIPDLGIGSPKNKKNDDEK"
     gene            2281..2823
                     /locus_tag="VFH96_07485"
     CDS             2281..2823
                     /locus_tag="VFH96_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973854.1"
                     /translation="MKAIATKLLITAIVFTTANAAPAKGDDFNSVVKMIEQFYRVKHV
                     GIPFLAKAGMKVGTTAARIKGGQARRIAEAGSIKLAVFEDQDFNGDFTKFRSSLNEAL
                     SATWMPLIQTLSAQDEEQVYIFLKDAGEKVNVLVITIEQRDATVVQVTLSPKNLALLL
                     KDPEGTGKSITQEATINDQE"
     gene            2827..4068
                     /locus_tag="VFH96_07490"
     CDS             2827..4068
                     /locus_tag="VFH96_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973855.1"
                     /translation="MYRYRFPLFLLFILATFASLPVSAQEVEDTIKIKTRVVFLDALV
                     KDKKTNLPISNLTTENFQVLDDGKPRNISYFTREGQARKPLALILILDLREDGAGRFL
                     KRSEILKSMEDELAKLPPGDEVAIMAMDMNNENEERLWLTEFTNDRAKLAAALARVPA
                     MTVSHEGQPSETKSTAAAKTDDSAGKPDDVVETEKIIGRNGGTVIRETHRDGSVNVKR
                     TNKKGDVTIELGDVYDMAAAVKDATRKAEATRPNSQLSIVWVSDGIAPIFFEDRDATE
                     QILIRNNAIFNSLTVELRTLFKFLLPVGKPIAGWLGMSLYGSAKHLAGRSGGEAVKVG
                     RPKDYGAGLSRIIGNLTARYSLGFALAEDEIDDGRMHSLEVRVKAQDEKGKTRKLQVS
                     SRQGYYMSSTIPKDAAAKAQY"
     gene            complement(4131..>4722)
                     /locus_tag="VFH96_07495"
     CDS             complement(4131..>4722)
                     /locus_tag="VFH96_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET6973856.1"
                     /translation="GAFTLLAAYKTFPAGTIHVGVVDPGVGSERRPIIVSANEQFFVG
                     PDNGLFTYICDREPSHRLVHVTSDHYFRRDPSTTFHGRDIFAPVAAALSNGVALEDFG
                     AEINDAVRLPSLETPLRIIHIDRFGNCVTNITRAEKSLVINGQKISEFRQFYGEGDDE
                     SLFAILGSAGFLEISVNGGSAAKVLGARRGDEVGFT"
BASE COUNT         1161 a         1315 c         1284 g          962 t
ORIGIN      
        1 ggcagcgttg ctatttctcg cggccctcgc ggaagaagcc atgttccgcg gctatggttt
       61 gcaaaccctg acccgcgcaa agctcgcctg gctcggcata ctgctgacgt cagtaccgtt
      121 tgggctcgtt catctcaaca atccgaacgt cgttccggtt gtgacagtta ccaacacggt
      181 gctcgccggc atctggctgg ctgcagctta cctgcgcact cggagtctat ggcttgctgt
      241 tggggttcac tggtcgtgga attgggcgct cggctggttc ttcggactac cggtcagcgg
      301 catgacagtc gtttccaatc ctcttcttga agctcaagat atcggaccta aatggttaac
      361 gggcggtact tacggtattg aaggcggcat tgcctgcacg atcgcgctga ttttattcac
      421 agccttcctg tggaaaactc gttggctctc ggccacaccc gagctcaagc aaatgacctc
      481 ggaagaaaac cctgcgacac cagagcccgt aataagcatc cgtcccgcaa acgataacgc
      541 ttaggtctgt taacagtctt caactctctc ccgtcttaat cgtgtccttg aacttcggtt
      601 tacccgatcg agacgccgat gtacgccacc gccgacatgc tactgacgag cccccaagca
      661 cacgccgagc aatcgcaaga gccggcgatc gaagtcgtgc tggtcagagc ggttttgcaa
      721 ggagaccgtg agggattctc gcggctctac gatctctacg caccactcgt acacggcatt
      781 ctgctggctc gcgtgccacg cgccgaagtt gccgatctcg tccaggacat ctttttccac
      841 gccttcaaga agctgcacac gttgcgcgac gaggcggcat tcgggccgtg gatcgcgatg
      901 atcgcccgca accgcgccgt cgatttccat cgccgctcga aagagatggt ggagatcaac
      961 gacgaccttc gcggcagcga ccaacacgac tcgcgagccg cagaaatcct cgagctgatc
     1021 cgcagcctgc cggaagcgta tcgcgaaact ctggtgttgc gcctggtcga gggcatgacg
     1081 ggaccggaga tcgcagcgcg caccggactc accgccgcgt cagtgcgagt caacctgcat
     1141 cgcggcatga agctgctgcg cgaacggctc ggatttatgg agggcttatg aacgacgatt
     1201 acctgtggga caaaaccggt gagcccgatc cacagattca acaactggaa gagatcctcg
     1261 gcacgctgcg ttatcagccc aggcctttgg tgcttccgcg taaacggaat tacttcccac
     1321 tgctggcgat tgccgcatcg ttactgctcg cgctactcgc cggagggatc tggttgcgcc
     1381 tacgttcgcg taacgaagtg ccaccacgac aagcgcgcat agaaacacca acacccgcgc
     1441 ccaagcgaga tgtaacgccg gtattgcctg aaaagaaatc cgcggttaat ccatctccgg
     1501 tcgtcgcaaa caacagacgc cgtcacagat ccgtgccatc agcggccgaa cgcgaagaag
     1561 cgctcatggc gaaacaacaa ctgatgctcg cgctgcgact gacaactgaa aagctacatt
     1621 tggtccgcac aaggacccaa ccgaaccaga tcaaaaacca acatcgagtt ggataaacgg
     1681 agcaattatg aaaacacttt tccacatcaa tatcagaacc gcactgattg ccgcgctgct
     1741 cgttctcgcg ccgatcttcg cggcccaggc tcagcagggc tcgcgcatac aaatgagtgc
     1801 cctcgatcat ctcgcggcga aagccagtca aactgtcgac gtcaatatcg acgagcgatt
     1861 gatgcgcctg gccgcgcggg tcttcgacga taaggatgaa gacgaacgcg aaatcaaaaa
     1921 actcatcgca aatctcaaag gtgtttacgt caagagtttt gaatttgaga ctgacgggca
     1981 atacgtggcc gccgatcttg agccgattcg cacgcagctc cgcggaccag gctggacgcg
     2041 gctggtaaac gtcaccagca agaaagaagg aatgctcgag gtttacctgc tcttcaatgg
     2101 cgacgttgtt ggcggcttgg cagtgcttca taccgatgat aaagagctca cggtcgtgaa
     2161 catcgtcggt ccggtcgatg tcgacaagct cgcgagactc gaaggacagc ttggcatccc
     2221 ggacctggga atcggatcgc caaagaataa aaagaacgac gacgagaagt gaggtgcgcg
     2281 atgaaagcga ttgctacaaa acttctcatc accgcgatcg tcttcacgac cgcgaacgcc
     2341 gctcctgcca aaggtgacga cttcaattca gttgtgaaga tgatcgagca gttctaccgc
     2401 gtaaagcacg tcggaattcc cttcctcgcg aaggcgggaa tgaaagttgg cactacggcg
     2461 gcacgcatca aaggcggcca ggcgagacga atcgccgaag cgggcagcat taagctcgcg
     2521 gtgtttgagg atcaggattt caacggcgac ttcacgaagt ttcgctcttc gttgaatgaa
     2581 gcattgagcg cgacgtggat gcctttgatc caaacactct cagcccagga cgaagaacag
     2641 gtctacattt ttctcaaaga cgcgggcgag aaagtaaacg tgctggtgat cacgatcgag
     2701 caacgcgacg ctaccgtggt ccaggtgacg ctctctccga aaaacctcgc gctgttgctc
     2761 aaagatccgg aaggtacagg taagtccatt acacaggaag caacgattaa cgatcaggag
     2821 tagatcatgt acagataccg tttcccactg ttcctacttt tcatccttgc gacgtttgct
     2881 tctctccccg ttagcgcaca ggaagttgaa gacactatca agatcaagac gcgcgtcgtg
     2941 tttctggacg cgctcgtgaa agacaagaag actaatctcc cgatttcgaa tctgactact
     3001 gagaattttc aggtgctcga cgacggcaag ccgcgcaaca tttcttactt cacgcgcgaa
     3061 ggccaggcgc gcaagccact cgctctgatt ctgattctcg atctgcgcga agacggcgct
     3121 ggccgttttc tcaaacgcag cgagattctc aagtcgatgg aagatgagct ggccaaactg
     3181 cctcccggtg atgaagtcgc gatcatggcg atggacatga ataacgaaaa cgaagagcgg
     3241 ctttggttga ctgagtttac gaacgaccgc gcgaagctcg cggcggcact cgcgcgtgtg
     3301 ccggcgatga ctgtgtcgca cgaaggccag ccgtcagaaa ctaaatcaac ggctgctgcg
     3361 aagactgatg atagcgccgg caagcctgac gacgtggtcg aaaccgaaaa gataatcggt
     3421 cgcaatggcg gcactgtgat ccgcgagacg catcgtgacg gtagcgtcaa tgtgaaacgc
     3481 acgaataaga aaggcgacgt gacgatcgag cttggtgacg tctacgacat ggctgctgca
     3541 gttaaagatg cgacgcgcaa agcggaagcg acgcggccga attcgcagtt gtcgatcgtg
     3601 tgggtatcgg atggtattgc gccgatcttc ttcgaggatc gtgatgcgac tgaacaaatc
     3661 ctcattcgca acaatgcgat cttcaactcg ctcacagtgg agctgcgcac gttgtttaag
     3721 tttctgctgc cggtaggtaa accgatcgcg ggctggctgg ggatgagttt gtatgggagt
     3781 gcgaaacacc tcgccgggag atcgggcggc gaggccgtga aggttggacg cccgaaagat
     3841 tatggtgctg gattgtcgcg aattatcggg aacttgacgg cgcgctacag tctcggtttc
     3901 gcgttagcgg aagatgagat cgacgacggc cgcatgcaca gtctggaagt tcgcgtgaaa
     3961 gcgcaggatg agaaaggcaa gacccggaag ctgcaggtga gctcgcggca gggatattac
     4021 atgtcgtcaa ctattccgaa ggacgccgcc gctaaggctc aatattagac aggatgacag
     4081 gatttgcagg attgacagga tctatcttgt taatcctgaa aatcctgtaa tcatgtaaaa
     4141 ccaacctcgt caccacgcct ggcgcctaat actttcgcag ccgatccacc attcaccgag
     4201 atctcaagaa atccagcact ccccaagatt gcaaacaacg actcatcatc accttcgcca
     4261 tagaactgtc gaaactcact aatcttctgt ccattaataa caagactctt ctctgcgcgc
     4321 gtgatgttcg tcacacaatt cccaaagcga tcgatatgaa tgatccgcaa cggtgtttca
     4381 agcgaaggca atcgaaccgc gtcatttatc tctgccccga aatcttccaa cgcgacacca
     4441 ttcgacagcg cagccgcgac cggcgcgaag atatcgcgac cgtgaaacgt cgtgctcgga
     4501 tcgcgtcgaa agtaatgatc ggaagtgacg tgaactaagc ggtgtgaggg ttcccgatcg
     4561 cagatgtaag tgaaaagccc attatcgggt ccaacgaaga actgctcgtt ggcactcacg
     4621 ataatgggcc gcctttcaga tccaactccc ggatcaacaa ctccaacatg aatcgtcccg
     4681 gcaggaaacg tcttgtaagc cgccagtaac gtgaacgccc ct
//
LOCUS       DASSFQ010000208         5030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_994255, whole genome shotgun sequence.
ACCESSION   DASSFQ010000208 DASSFQ010000000
VERSION     DASSFQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994255"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..368
                     /locus_tag="VFH96_07500"
     CDS             138..368
                     /locus_tag="VFH96_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6973857.1"
                     /translation="MPDKNDKVKENVNGSYYVDSSCIDCDVCRDTAPDNFMRCDENNY
                     SFVYKQPESDEERALCEEAVLCCPVEAIGNDG"
     gene            854..1822
                     /locus_tag="VFH96_07505"
     CDS             854..1822
                     /locus_tag="VFH96_07505"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET6973858.1"
                     /translation="MKRLFMIPALLAVVLSTAVASHAKEITVRGKLQKTVEAGGWLIV
                     ARDTKYLLLNAKDFQSNSWFKESTNVEAVGETKDVMTTFMEGTPFEAKSLKPYEQQAT
                     AGSDDNRRVITRVMVAGDSIVQAQPDTAILTISVVTQARNALDAQQQNATKTDAVVRA
                     LKAAAGAGAEIKTSGYSLQPQRVYKENQPPTISGYEARNSVTVTTGDLNKVGTIIDAA
                     AAAGSNEVQGIAFTLRQDRPARDRALSEATREAVSKAQVIASALGGRVVRIIEVQEEG
                     FQQRPPTPIYRTESFAKADMASTPIEVGSLEISSRVQLIAEVETNL"
     gene            1958..2737
                     /locus_tag="VFH96_07510"
     CDS             1958..2737
                     /locus_tag="VFH96_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6973859.1"
                     /translation="MARYRNDMDDERDYYPREERLRRENFGAYDEQPYRPRREFDTER
                     RPDYNRGRFGERANYESDEPRYAEYRSERPTTAREREYYAPERSRLRCREIMTRDLAV
                     ATRDTTLPEIALMMKQEDTGVIPIVEYDTQNGNGRVETQERKYEGRNYSRGKLIGLIT
                     DRDIVVRAVADNKDCASVRAEDVMSVDIHTAKPNDRVVDVIKKMGDKQVRRIPVANDN
                     GYLVGMISMADIALETRQDRELAEAIEEISKGSSFWNRIFG"
     gene            2835..3197
                     /locus_tag="VFH96_07515"
     CDS             2835..3197
                     /locus_tag="VFH96_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6973860.1"
                     /translation="MRNTNREFSLIRAGRRVLPSGRLWNPAADVYQSADGWVVKVDLA
                     GICSDELEIEIRDSLLSIRGCRRDTHYREGFVYHQMEITYSRFEKSIEFPAPIEGASI
                     RHDYSDGFLIINVRREQD"
     gene            3200..>5030
                     /gene="lon"
                     /locus_tag="VFH96_07520"
     CDS             3200..>5030
                     /gene="lon"
                     /locus_tag="VFH96_07520"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6973861.1"
                     /translation="MPEPLENASPAPETPTAPVETTDLRFAAHEAGQPFEVAVLALQN
                     LTIFPETVIPLGVGRPRSLAAVESALSTPEKLLCCITVRPDRSDDTDAKPADLYDVGT
                     LVMIKRMERTQDGMRIIVQGTERVRVSQWKQEDPHLRAIVEILPEPRIVDPEQVEAAK
                     RNLQQMITEALALLPNVPPEVRIAVLGQVDAVRLSYFLASILTLGVEEEQKMLEANTA
                     DELARLANANLAREVEILQLRSKIASEAQVEMDKAQRDYVLRQQMRAIQKELGEDESG
                     EAAENDVLRERFEKAELPPEVRAEADRELKRLQRLPAAAPDYQVIRTYLEFILELPWL
                     KSSEDKLDLAEARRILDEDHFGLEDVKERILEFLAVIKLRPDTKSPILLFVGPPGVGK
                     TSLGRSIARALGRQFERMSLGGVRDEAELRGHRRTYIGALPGRIIQSIRRAGVNNPVL
                     MLDEVDKLGADYRGDPAAALLEILDPQQNNSFRDHYLDLPFDLSKIFFIATANQLGPI
                     PAPLRDRMEIIRLAGYSDREKLEIARRYLIPRQTTENGLNNAQLKITDDAVELIAARY
                     TREAGVRQMERSVGSIARKVALKIAQGQAESVTVTAADLRDYLG"
BASE COUNT         1235 a         1343 c         1424 g         1028 t
ORIGIN      
        1 aggttgcgaa gaacgctcac aagaaaaaca tcagtttgcg ggaatcggcc gtggaactcg
       61 gttacctgac tgaggaacag tttgacaagc tcgtgatccc ggaagacatg acgcatccct
      121 gattagcaga ttctgaagtg cccgataaaa acgacaaagt aaaagagaac gttaacggtt
      181 cgtattatgt ggactcgtcg tgcatcgact gcgacgtttg tcgcgacacc gcgcctgaca
      241 acttcatgcg ttgcgacgag aacaactact cgtttgttta caagcaaccg gagagtgatg
      301 aagagcgagc gctttgtgag gaagcggtgt tgtgttgtcc ggttgaggcg ataggcaacg
      361 acggatgatc ttagccgcgg atttacgcgg atgaacgcgg atcagaaaat atcttaagaa
      421 gaccctcttc aacttcaacg ctgtacgttc gaatcctatc cggcaccgtc aaacattctc
      481 cactccgcac atcaaactgc cacccgtgta gtccacactc aataatgtgc ccacacagcg
      541 ttccttccgt cagccgcgcg cctttgtgcg gacagaaatt ctcggtggca tagtattcgc
      601 catctacgtt gtacacagct aattcgttgc cgtcaggcaa ctcaaagctg atgcaagtgc
      661 cggggtgcaa gtctgcctta gtatcgaatg attgcatcgg atggatggta acaaaacacg
      721 acgtgcacaa ccagaatcac aaatgctgca aatacaagga agcaagtttc cgtctctcgc
      781 gcgttctcta tctttgctgc cggtttcaac tctcccacgg ctgtgagatt ctaggaccag
      841 aagaggagag attatgaaac gactattcat gattccagcg ctgctggcag ttgttctcag
      901 cactgccgtc gccagccacg caaaagaaat caccgtacgc ggaaaacttc agaagaccgt
      961 cgaggcaggc ggctggctga tcgttgcccg cgacacgaaa tatttacttc ttaacgcaaa
     1021 agactttcaa agcaattcgt ggtttaaaga gtcgacaaac gtcgaagcgg tgggcgaaac
     1081 aaaggacgtg atgaccacct ttatggaagg cacacctttc gaagccaaat cgctcaaacc
     1141 atacgaacaa caagcaaccg ccggcagcga cgacaaccgg cgcgttatca cgcgcgtgat
     1201 ggttgccggc gattccatcg tccaggcgca gcctgacacg gcgattttga cgatttcagt
     1261 cgtcactcaa gcccgcaatg cgctcgacgc gcaacagcaa aacgcgacga aaaccgacgc
     1321 cgtcgtgcgt gcgttgaaag cagcggccgg cgccggcgct gagatcaaaa ccagtggcta
     1381 cagcctgcaa ccgcagcgtg tttacaaaga gaatcagcca cccacgatca gcggttacga
     1441 agcacgaaac agcgtcaccg tcacgaccgg cgatctgaac aaagtcggca cgatcatcga
     1501 cgccgctgcg gcagccggat caaacgaagt gcagggcatc gcatttacgt tacgacagga
     1561 tcggcccgct cgcgatcgcg cgctgagtga agcgactcgt gaagcggtga gcaaagcaca
     1621 agtgattgcg tcggcgctcg gtggacgcgt tgtgcggatt atcgaagttc aggaagaagg
     1681 atttcagcag cggccgccaa cgccgatcta tcggactgaa tcgtttgcaa aggcagatat
     1741 ggcgtcgacc ccgattgaag ttggatcgtt ggagatctct tcgcgagtgc aactgatcgc
     1801 ggaggttgaa acgaacctat gaaagcattt cacgcaaagg cgcaaagaga aaacaagaca
     1861 ctttctttgc ggctttgcgt gaactttgcg cctttgcgtg aaaagttctt cccccgaagc
     1921 ccccaagaga ccgaggttcc tggtaaggag ggaagcaatg gccagataca gaaatgatat
     1981 ggatgacgag agggactatt atccgcgaga ggagcgcctc agacgggaaa acttcggcgc
     2041 ctacgacgaa caaccgtatc gacctcgccg tgaatttgat accgaacggc gacccgacta
     2101 caaccgtggc cgttttggtg aacgagctaa ttacgagtcg gacgaaccgc gttacgcaga
     2161 atatcgcagc gaacgtccga ctacggcgcg cgagcgagaa tactacgcgc ccgaacgttc
     2221 cagattgcgc tgccgtgaaa tcatgactcg cgatctcgca gtcgccacac gcgacaccac
     2281 tcttccggaa atcgccttga tgatgaaaca ggaagacacg ggcgtaattc cgatcgtgga
     2341 gtacgacacg caaaacggca acggccgagt agagactcaa gaacggaaat atgaaggccg
     2401 caattacagt cgcggcaagc taatcggctt aatcactgac cgcgacatcg tcgtgcgcgc
     2461 cgtggccgac aacaaagatt gtgcgagcgt gcgggccgaa gacgtcatgt cggttgatat
     2521 tcacaccgcg aagccgaacg accgcgtcgt cgatgtgatc aagaaaatgg gagacaaaca
     2581 ggtgcgacgt attccggtgg ctaatgacaa cggctacctg gtcggcatga tttccatggc
     2641 cgacatcgca ctcgaaaccc gtcaggatcg cgaattggct gaagcgatcg aggagatctc
     2701 gaagggttca tctttctgga accggatctt cggataattt ttttgccacg gataaacgcg
     2761 gatatcacgg atctaatctg tggtctctgt gggcatccgt ggcaaatttt cttaattatt
     2821 cgtattctct tgccatgcga aataccaatc gtgagttctc gctgatccgt gcgggccggc
     2881 gagtgttacc gtcgggccgc ctctggaatc cggccgcgga cgtctatcag tcggcggatg
     2941 gctgggtcgt gaaagtggat ctggcgggca tttgttcgga cgagttggag atcgaaatcc
     3001 gcgactcact gctgagtatt cgtggttgtc gacgcgacac acattatcgg gaaggctttg
     3061 tttatcacca gatggaaatc acctacagtc gctttgaaaa gtcgatcgag tttccggcgc
     3121 ccattgaagg agcatcgatt cgacacgact acagcgatgg ttttttgatc atcaacgtgc
     3181 gtagggagca ggattagtta tgccggaacc tcttgaaaat gcatcgccag cccccgagac
     3241 accaacggcg ccagtagaaa ctacggatct gagatttgcc gcacatgaag ccgggcagcc
     3301 ttttgaagtg gccgtgctgg cgctgcaaaa tctgacgatc ttcccggaaa cggtgatacc
     3361 tctgggcgtc ggcaggccgc gttcgctcgc cgccgttgaa tccgccctct caactccgga
     3421 aaagttgctg tgttgtatca cggtgcgtcc cgatcgttcg gacgatacag acgcgaaacc
     3481 ggccgatctg tatgacgtcg gcacgctcgt catgatcaag cgcatggagc ggacccagga
     3541 tgggatgcgc attatagttc agggaacgga acgcgtgcgc gtgtcgcagt ggaaacagga
     3601 agatccacac ttgcgcgcga tagtcgagat tctacccgag ccgcgtattg tcgatccgga
     3661 acaagttgaa gcggcgaagc gcaatctgca acagatgatc acggaggctt tggcgctgtt
     3721 gccaaacgtg ccgcctgaag tgcgaattgc tgtgctgggg caggtcgatg ctgttcggct
     3781 ttcgtatttt cttgcctcaa ttctgactct cggcgttgaa gaagagcaga aaatgctcga
     3841 ggcaaacact gctgacgagc tcgcgcgtct cgcaaacgcc aatctcgctc gcgaagtcga
     3901 aatactccag cttcgttcga agatagcttc cgaagcacaa gttgagatgg ataaggcgca
     3961 gcgcgactac gtgctgcgtc agcagatgcg tgcgattcag aaagagctcg gcgaagacga
     4021 gtccggcgaa gctgcggaaa atgatgtgct gcgcgagcga ttcgagaagg ctgaactgcc
     4081 gccagaagtg cgggccgagg ctgatcgcga gctgaagcga ctgcaacgac tgccggccgc
     4141 tgcgccagac tatcaggtta ttcgtaccta tctcgagttc attctcgaac tgccgtggct
     4201 caagtcttct gaagacaaac tggatctcgc cgaagcgcgc cggatcctgg acgaagatca
     4261 cttcggtttg gaagacgtca aagaacgcat tctggaattt ctggccgtaa tcaaactccg
     4321 gcctgacaca aagagcccga tactgctgtt tgtcgggccg ccgggagtcg gcaagacttc
     4381 cctcggccgg tcgattgcgc gcgctttagg aagacagttt gaacgaatgt cgctgggcgg
     4441 cgtgcgtgat gaggcagagt tacgcggtca tcgtcgcacg tatatcggcg cgttgccggg
     4501 tcgaatcatt caatcgattc gacgcgccgg cgtgaacaat cccgtgctga tgctggacga
     4561 agtcgacaag ctgggcgccg attatcgtgg cgatccagcc gcggcactgc tcgagattct
     4621 cgatccgcaa cagaacaatt cgttccgcga tcactatctt gatctgccgt ttgatctctc
     4681 gaagatcttc tttatagcga cggcgaacca gctcggcccg attcccgctc cgctgcgcga
     4741 ccgcatggag atcatccgtt tggccggtta tagcgatcgc gagaaactcg agatcgcgcg
     4801 ccgctacttg atcccgcgtc agaccaccga aaatggtctg aacaacgcac agctcaagat
     4861 cacggacgat gccgtcgagc tgatcgccgc gcgctacacg cgcgaagccg gcgtgcgcca
     4921 gatggagcgg agcgtcggca gcatcgctcg taaagtggca ctgaagatcg cgcagggtca
     4981 ggcagagtca gtcacggtta cggctgccga tcttcgtgat tatctgggtg
//
LOCUS       DASSFQ010000209         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_692977, whole genome shotgun sequence.
ACCESSION   DASSFQ010000209 DASSFQ010000000
VERSION     DASSFQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692977"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="VFH96_07525"
     CDS             complement(<1..1478)
                     /locus_tag="VFH96_07525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6973862.1"
                     /translation="MKPDRWRKVDELFEAALEHEPARRGAFLDEACGSDKDLRDEVEK
                     MLDFEKRSEDFIKTDVFDVAAKLITQPRAKISTTSDSIDDARFVSGDVLLERYRIVGL
                     LGRGGMGEVYRADDLKLKQAVALKFLPASLSADGAALARFYKEVSVARQISHRHVCRV
                     YDVSEFQGEHFISMEYVRGEELSSLLKRIGRLPQDKAIDVARQLCAGLAAVHERGVLH
                     RDLKPANIMLDEHGEVRITDFGIAALASETDRREMSGTPAYMSPEQLDSRELTTRSDI
                     YSLGLVLYEVFTGKKAFEASSMQDLLRLRRSDTTPTSPAQYVPDLDPLIERVIFRCLE
                     RDPAKRPGTTLQVAAALPGGDPLAAALAAGETPSPEMVAAAPRLGGLKPAVAVSLLAA
                     AVILLVATCLLSRTTTLYQQVPLNKSPEVLKDRAAELVKKFGYMATPLDTAYGMGLDR
                     AYLKYVHDNDSSPGRWNRLRSGQPAAIYFWYRQSPRPFEVNDG"
     gene            complement(1447..2070)
                     /locus_tag="VFH96_07530"
     CDS             complement(1447..2070)
                     /locus_tag="VFH96_07530"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6973863.1"
                     /translation="MAEFRAMPEMSPQEVTQLLADWGKGDRSALDRLFPLVHSELRRI
                     AQRQMNQERAGHTLQATALVNEAYLKLVGQQASPWQNRAHFFAVCAQVMRHILIDHAR
                     AHRRDKRGGGAIQVSLNDVAILAEDQSSTVLALDEALRQLETLDPQKGKIVELRYFGG
                     LSIEETAEVLNISPRTVRREWQRSKVWLYRMISEGIENETRPLAKSR"
     gene            2176..>2375
                     /locus_tag="VFH96_07535"
     CDS             2176..>2375
                     /locus_tag="VFH96_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973864.1"
                     /translation="MKTLVFVTLLMFGFSGQTVGTMDSRAADEAALRESVKQLETGWN
                     TKSGALFAKPFAEDADYVVING"
BASE COUNT          477 a          641 c          719 g          538 t
ORIGIN      
        1 ccatcattta cctcaaacgg acgcggactc tgtcgatacc agaaataaat cgcggccggc
       61 tgaccgctgc gcaatctgtt ccagcgtccc ggtgatgaat cgttatcgtg aacgtacttg
      121 agataagcac gatcgaggcc catgccgtat gcagtgtcca atggagtcgc catgtaacca
      181 aacttcttca ccagttcagc ggcacgatcc ttcaagactt caggcgactt gtttagtgga
      241 acttgctggt acagggttgt cgtgcgcgat agcagacagg tggcgacgag caggatcact
      301 gcggcagcga gtaacgaaac cgccaccgcc ggtttcaaac cgcctagtct cggagccgcc
      361 gcgaccatct ccggtgatgg agtttcaccg gctgcaagcg cggccgcgag tggatcgcca
      421 ccgggtaatg ctgctgcaac ttgaagggtt gttcccggtc gtttcgccgg atctctttct
      481 aagcaacgaa agatgacgcg ctcgatcaat gggtcgagat cgggtacgta ctgcgctgga
      541 ctcgtcggcg ttgtgtcgct ccggcgcaga cgcagtaagt cctgcatgct cgaagcctca
      601 aacgctttct tgccggtgaa tacttcatac agcacgagtc cgagcgaata gatgtcgctc
      661 cttgtggtta actcgcgact atcgagttgt tcgggcgaca tgtacgcagg cgtgcctgac
      721 atttcgcgac gatcagtttc gctggcgagt gcggcgatgc cgaagtccgt gatgcgtact
      781 tcgccgtgct cgtcgagcat gatgtttgcc ggtttcagat cgcgatgcag cacgccacgt
      841 tcgtgtacgg cggcgagacc ggcgcagagt tgtctcgcca cgtcgatcgc tttgtcttgc
      901 ggcagacggc cgatgcgttt gagtagcgat gagagttctt cgccacgaac atactccatc
      961 gagatgaagt gctctccctg aaactcggac acgtcgtaaa cacgacacac gtggcgatgc
     1021 gagatctgac gcgcgacgct cacttctttg tagaagcgcg cgagcgccgc accgtcggcg
     1081 ctgagtgaag cgggcagaaa cttgagtgcg acggcctgtt tgagttttag atcgtcggcg
     1141 cgataaacct cgcccatacc gccacgacca agcaggccga cgatgcgata gcgctcgagg
     1201 aggacgtcgc cggagacgaa tctcgcgtcg tcgattgagt cggatgtcgt tgatattttg
     1261 gcgcgcggct gggtgattag tttcgcggcg acgtcgaaga cgtcagtctt gatgaagtct
     1321 tccgagcgct tttcgaagtc gagcattttc tcgacctcgt ctcgaagatc cttgtcgctg
     1381 ccgcacgcct catcgagaaa cgcgccgcga cgggccggct cgtgctcgag cgcggcttca
     1441 aagagttcat cgacttttcg ccagcggtcg ggtttcattc tcgatccctt cggagatcat
     1501 ccggtagagc catacttttg agcgctgcca ctcgcgtcgc accgttcgcg gtgagatgtt
     1561 taggacttct gctgtttcct cgatgctcaa accgccgaag taacgcagct cgacgatctt
     1621 gcctttctgc gggtcgaggg tttcgagctg gcgcagtgct tcatcgagag ccaggacagt
     1681 cgacgactga tcttccgcga gtattgcgac gtcgttgagt gaaacctgaa ttgcgcctcc
     1741 accacgtttg tcgcgccggt gtgcacgcgc gtgatcgatc aagatgtggc gcatcacctg
     1801 ggcgcagacg gcgaagaagt gcgcgcggtt ttgccatggt gaagcctgct ggccgacaag
     1861 cttcaggtaa gcctcgttca cgagcgcggt tgcttgcaag gtgtgacccg cgcgttcctg
     1921 attcatctgg cgttgagcga tccgccgtaa ctccgaatgc accagtggaa agagtctatc
     1981 gagtgcggag cggtcgcctt taccccagtc ggcaagcagt tgagtgactt cctgcggcga
     2041 catctcgggc atcgcgcgaa attctgccac aaagaggcac aaaaagcaca aaaacagatt
     2101 ggtggccgct tttgttttct tttgtcgctt tcggaggtga gtggcgatgg ggccacaaat
     2161 taacggagga tactcatgaa gacattggta tttgtaacgt tgcttatgtt cggtttttcg
     2221 gggcagacag ttggcacgat ggatagtcgc gccgcggatg aagcagcgtt gcgcgagagc
     2281 gtcaaacagt tggagacggg ttggaatacg aagagcgggg cgttatttgc caagccgttt
     2341 gccgaggacg cggattacgt ggtcatcaat ggcag
//
LOCUS       DASSFQ010000210         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_693799, whole genome shotgun sequence.
ACCESSION   DASSFQ010000210 DASSFQ010000000
VERSION     DASSFQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693799"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1094
                     /gene="selD"
                     /locus_tag="VFH96_07540"
                     /pseudo
     CDS             102..1094
                     /gene="selD"
                     /locus_tag="VFH96_07540"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            1191..2534
                     /gene="egtB"
                     /locus_tag="VFH96_07545"
     CDS             1191..2534
                     /gene="egtB"
                     /locus_tag="VFH96_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET6973865.1"
                     /translation="MASTIREISRQDSVSIAELYKQVRARTMQIVAPLEIEDYVIQTA
                     EFMSPPRWHIGHTSWFFETVLQAHKPGYRVYSEDFLFYFNSYYEGFGVRIERPKRGTK
                     SRPTVKETVAYRNHIDEQVLQLLQSPDNLSETLLSLVRLGLEHEMQHQELLVYDIKHL
                     LCDQFDVSMQPLPQPSTTVEAMAEIEGGLFWLGYDHAGANLSYDFAFDNEKPAHQVFL
                     QDYALDKAPVSNGDFLEFINDGGYQNFRWWFSEGWTTVNNEQWRAPLYWELHDDEWMI
                     RDFAGLHPATSKKDEPVSHVSFFEASAFAKWAGKRLPTEAEWEKAACYDARRNVRTAF
                     PWGDTDPTSGKANLFENDFWSVAPIGAFPEGANAYGCQQMIGDVWEWTTSDYVPFPGF
                     KSEFDEYNDKWFVNQKVLRGGSFATPQLHIRATYRNFFHTNERWMLSGFRCAQDR"
     gene            2604..>3665
                     /locus_tag="VFH96_07550"
     CDS             2604..>3665
                     /locus_tag="VFH96_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET6973866.1"
                     /translation="MSPELRRVLAIFVFVVFAAGSALAQTEARSNANPEPAGPGSSTA
                     AKNTPVNPKYTTPKSPLDTAYGAKIAEYTTEKYFMTELVDHLPLSDKVPSPEKVLGYA
                     VGTPNKLTYTKDLYRYYRELAKASPRVRVLVAPEKSEEGREQMLVLVSDEENLAKLDR
                     YKEITAKLADPRKVTDAEAQTLISEGKPFYWASGSIHSPETGSPEMLMEMAYRLAVED
                     SPFIEDIRKNMIIMITPALEVDGRDRMVDLYNYRKANPGKPTPPLLYWGKYVAHDNNR
                     DGLGLALALTRNQMRTFLDYHPTVLHDLHESVPYLYTMTGTGPYNAWLDPLAIDEFQL
                     LAYHEIEEMTKRGVPGVWTH"
BASE COUNT          871 a         1028 c         1016 g          750 t
ORIGIN      
        1 ttgccattcg cttaaactag ccttgtccaa gattacagga aggaggtgat gcgatgcgac
       61 tgacggaaat ggtgtcgtgt tccggttgag cggcgaagct tagcccgagc ttactcgctc
      121 aggttctgag cgggctaccg aaacaacctc atgatccgaa tttgattgtc ggttttgaaa
      181 cggccgacga cgcaggtgta taccgccttc gagacgacct cgcaatcgtc caaacgctcg
      241 atttcttcac gcctattgtt gacgacccgt acgactacgg ccgcatcgcc gcgctcaact
      301 ctatcaacga cgtttgggcc atggccggaa cgccgatcac cgcgatggca atcacgtgct
      361 ttccgaagaa aggcgtcgat ccggcgattc tgggtgagat catgcgcggc gggttggaga
      421 ccatcaacaa gtatggcgtg acgttgattg gcggacacag cgttgataac gagcagatca
      481 tgttcgggta ttcggttacc ggtgtgattg atccgaacaa agtggcaaag aattcgggcg
      541 cgcgagcggg tgatgtgatt attttgacta agccgatcgg aacgggcgtg atttccaccg
      601 ggatcaagaa ggggcgcgcg agtgattcgg tcgttgcagg ttcagttgag acgatgctga
      661 cgccgggcaa gttcgccgcc gaagcgatgc gtgaatttgg cgtgaagggc gcgacggatg
      721 tgacgggctt tgcgctgatg ggtcatgcat gggaaatggc gtgcgcgagt aaagtcacga
      781 tcgaaatcga tgcgagtgcg gtgccgctac tcgacggtgc cttggaaatg gcgtcgcagg
      841 gaatgctgac cagcggtgac aaaacgaatc gtgaatacgt aggcgcagat gtcgagattg
      901 ctgaagctgt cgatccgaac atcgtcaagc tgcttttcga cccgcaaacg gctggtggga
      961 tgctgatcgc gatcgcagaa aacaaagctg aggctctcct ccgcacgctc aacgaacatt
     1021 actcgcacgc gcgcatgatc ggccgcgtgc tccccgaaag cccccgaaag attatcgtgt
     1081 ccgataagct ttagcttgtc gtaaggtcgt aaggtcgcga caagctaaag cttatcggac
     1141 atccgattac gcttttcgag ccgcagcggt tattattaac ccaatcaatc atggcttcga
     1201 caatccgcga gatctcaagg caggattccg tctctatagc ggagctttac aagcaggtgc
     1261 gcgcccgcac gatgcagata gtcgcgccgc tcgagattga agactacgtc atccagaccg
     1321 cggaattcat gagtccgccg cgctggcaca tcggccacac gtcgtggttc ttcgaaacag
     1381 tactgcaagc gcataagccc ggttaccgag tctattccga agacttcctc ttctacttca
     1441 actcgtatta cgaaggcttc ggcgtgcgca tcgagcgtcc gaaacgcggc acgaaatcgc
     1501 ggccgacggt gaaagaaacc gtcgcttacc gcaatcatat cgacgaacaa gtactccaac
     1561 tgctgcaatc accggataat ttatcggaga cgctgctgtc gctggttagg ctcggcctcg
     1621 aacacgagat gcagcaccag gagctactcg tctacgacat caaacatctc ctgtgcgatc
     1681 agtttgacgt ttccatgcag ccactgccgc agccgtcgac caccgttgaa gctatggctg
     1741 aaatcgaagg tggtctgttc tggctaggtt atgaccacgc gggcgcgaac ctttcttacg
     1801 acttcgcatt cgataacgag aagcccgcgc accaggtctt cctgcaggac tacgcgctcg
     1861 acaaagcccc cgtcagcaac ggcgactttc tcgaattcat caacgacggt ggttatcaaa
     1921 acttccgctg gtggttctcc gaaggttgga caacagtcaa caacgaacag tggcgcgcac
     1981 cgctttactg ggaactgcac gacgatgagt ggatgattcg agacttcgcc ggtctgcatc
     2041 ccgccacgag taaaaaagac gagccagttt ctcacgtcag ttttttcgaa gcctcagcgt
     2101 ttgcgaagtg ggccggcaag agactaccaa ccgaagcgga atgggagaag gccgcgtgtt
     2161 atgatgcccg ccgcaacgtc aggacggcat ttccgtgggg cgacactgac cccaccagcg
     2221 gcaaagcaaa tcttttcgag aacgatttct ggtcagtcgc accgatcggc gctttcccgg
     2281 aaggcgcgaa cgcttacggc tgccagcaga tgatcggcga cgtgtgggag tggaccactt
     2341 cggactacgt gccattcccg ggattcaagt cagagtttga cgaatacaac gacaagtggt
     2401 tcgtcaatca aaaggttttg cgcggcggct cgtttgctac gccgcagttg cacatcagag
     2461 ccacgtaccg aaacttcttc cataccaacg agcgctggat gctttcggga tttcggtgtg
     2521 cacaggatcg gtaacacgga ttagacggat cctaaaagaa gatctacgcg gatcagagcc
     2581 tacctaactg aaggagaaac atcatgtccc cagaactccg aagagtgctc gctatatttg
     2641 tgtttgtggt gtttgctgcc ggttcagcgc tcgcgcaaac tgaggcgcgc agtaatgcga
     2701 atcctgaacc cgccggcccc ggcagttcga ccgcggcgaa aaacacgccc gtcaatccga
     2761 aatacaccac accaaagtct ccgctcgaca ctgcttacgg cgcaaagatc gccgagtaca
     2821 cgactgaaaa gtacttcatg accgagctcg tcgatcacct gccgctgtct gacaaggtgc
     2881 cttctcctga aaaggttttg ggttatgcag tcggaactcc aaacaagctc acttacacga
     2941 aagatcttta tcgttactac cgtgagctcg cgaaagcgtc gccgcgcgtg cgcgtgctcg
     3001 tcgcgccgga aaagagcgaa gagggacgcg agcaaatgct cgtgctcgtg agtgatgaag
     3061 aaaacctcgc aaagctcgat cgttacaaag aaatcaccgc gaaactcgcc gatccgagaa
     3121 aggtcacgga cgccgaggca caaacgctga tttcggaagg caagccgttc tactgggcct
     3181 ccggctcgat ccactcgccc gaaaccggct cgcccgagat gctgatggag atggcctatc
     3241 gcctcgcggt tgaggactcg ccgttcatcg aagacattcg caagaacatg atcatcatga
     3301 tcactccggc gctcgaagtc gatggccgcg atcgcatggt tgatctctac aactatcgca
     3361 aagcaaaccc cggcaaaccg actccgccac tgctttactg gggcaagtac gtcgcacacg
     3421 acaacaaccg cgacggtctc ggactcgcac tcgcgcttac gcgcaaccag atgcgaacgt
     3481 ttctcgacta tcacccgacc gtgctgcacg acctgcacga atcagtgccg tacctttaca
     3541 cgatgaccgg cactggccca tacaacgcct ggctcgatcc gctcgcgatc gacgagtttc
     3601 agctactcgc gtatcacgag atcgaagaga tgacgaagcg cggcgttccg ggtgtttgga
     3661 cacac
//
LOCUS       DASSFQ010000211        16683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2039335, whole genome shotgun sequence.
ACCESSION   DASSFQ010000211 DASSFQ010000000
VERSION     DASSFQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16683
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039335"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..504)
                     /locus_tag="VFH96_07555"
     CDS             complement(124..504)
                     /locus_tag="VFH96_07555"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973867.1"
                     /translation="MDHRVIKAPRGAQLSCKGWHQEAALRCLMNNLDPDVAERPDELI
                     VYGGAGKAARNWASFDAIVRELRALENDETLLVQSGKPVAVFRTHEHAPRLDDVRTDD
                     CGQLDLYRHAGHRAGHVRDVCCNG"
     gene            complement(510..818)
                     /locus_tag="VFH96_07560"
     CDS             complement(510..818)
                     /locus_tag="VFH96_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET6973868.1"
                     /translation="MANGMDERRKGLEEEYFHRKNQEAIEKLREKMRVAAQAKEAGTS
                     SMKCPRCDGGTLKEIFIEDVPIDTCDQCAGVWLDSGEMEKLSKTDIARWFRSLWNQME
                     "
     gene            complement(846..2348)
                     /gene="hutH"
                     /locus_tag="VFH96_07565"
     CDS             complement(846..2348)
                     /gene="hutH"
                     /locus_tag="VFH96_07565"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET6973869.1"
                     /translation="MLQLSGQQLTLQQIVDIAEGREQVVLGSDARVRVERARQVVQDI
                     VTQGRTVYGVNTGFGKLSDVSIDRADLIQLQLNLVRSHSCGLGDPLSEPEARAMLLLR
                     ANVLASGFSGARPLVIDTLIAMLEKGVTPVIPEKGSVGASGDLAPLAHLALAAIGEGE
                     VFYEGERLPAATALQRAQIAPLQLEVKEGLALLNGTQAMGAVGALALHRAERLVQLAD
                     VAGAMTLESLKGTPVAFDERIHAARPHPGQLAVAAHLRDLLQDSEIRQSHLVGDPRVQ
                     DAYSLRCMPQVHGAIRTALDHAKQVVEIESGSATDNPLVFSETGEVLSGGNFHGAPLA
                     LVFDYAAIALTDLMSITERRIDRLVNPDSNEGLPPFLTSHPGISSGFMMLQVTAVALL
                     NEAKILAHPASIDNVPTDGGKEDHVSMGMTSATKMRAIVKLAEMATAIELMAGAQALE
                     YHAPLAPGRGVKQAYEIVRHHVAPLTQDRTMAPDIEKLTSAIIRGDFQSV"
     gene            complement(2352..3296)
                     /locus_tag="VFH96_07570"
     CDS             complement(2352..3296)
                     /locus_tag="VFH96_07570"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HET6973870.1"
                     /translation="MSTEASHRGSGQSTSDRVTGRLRSTPPAEPLTDINPQSPAALMR
                     RMSPGEFNTAMIHFYRGEVQRSNTWRNRLDTTTNWAVLTAGATLSFVFSSPNNPHFVI
                     PINSILVMIFLLMEARRYRYYEIWSSRVRILETGYFAHLLMPDSRPADESWAEHLAAD
                     LTTPHFTITEWEAVGRRLRRNYLWIFVLLALSWNLKVYLSPLPARDFNVFINRATVGI
                     VPGWIVFVVGIVFNAAVFGFAIGTVRLREATGEVLPKHEFSLRPFKRMTSWVPATTRR
                     TTVRAKRARQRVRSREQTAEFKKIDISETPETRAEVRS"
     gene            3498..3968
                     /gene="tadA"
                     /locus_tag="VFH96_07575"
     CDS             3498..3968
                     /gene="tadA"
                     /locus_tag="VFH96_07575"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET6973871.1"
                     /translation="MESELDEQWMRQALVAAREAQAIGEVPIGVCIVSENQLVAVCGN
                     RTRTDCDPTAHAEIVALREAARKFGNYRLTGVDVYSTIEPCAMCAGALIQARVRRLIY
                     GAPDERAGAVISRFRICDTDFLNHRIEISAGVLEAECRALMQEFFQTRRGRIVD"
     gene            3946..4035
                     /locus_tag="VFH96_07580"
     tRNA            3946..4035
                     /locus_tag="VFH96_07580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3980..3982,aa:Ser,seq:cga)
     gene            4139..4228
                     /locus_tag="VFH96_07585"
     tRNA            4139..4228
                     /locus_tag="VFH96_07585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4173..4175,aa:Ser,seq:gga)
     gene            4292..4813
                     /locus_tag="VFH96_07590"
     CDS             4292..4813
                     /locus_tag="VFH96_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET6973872.1"
                     /translation="MSDDKLAIEEARRAAQHESVKSQVEGEVQAEIGERAAVKSVPGE
                     SQRIDQVAGEFRSKAVNEVVESEREVERSRGLARISQIVDYIFWVIYALLGMRFLLAL
                     IAARSTAGFVQFIVAISNPFYAPFRGIVASPSTDSGHTLLLPIVVAIIAYALLHLAIN
                     GLLRMFAHRKTEI"
     gene            4823..4921
                     /gene="ffs"
                     /locus_tag="VFH96_07595"
     ncRNA           4823..4921
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFH96_07595"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4960..6672
                     /gene="dnaX"
                     /locus_tag="VFH96_07600"
     CDS             4960..6672
                     /gene="dnaX"
                     /locus_tag="VFH96_07600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET6973873.1"
                     /translation="MAYQVIARKWRPQTFDEVTGQEPITRTLRNAIEHQRLHHAYLFS
                     GARGVGKTTSARLLAKALNCHKAKEPTPTPCRSDDPDQCPSCREITESRSIDVQEIDA
                     ASNTGVDNVRDAIIASVGIAPARDRYKVFIIDEVHMLSGAAFNALLKTLEEPPPRVVF
                     IMATTERHKVPETILSRCQQFEFRTIATAKILERLKLIAKSEKITIPDDALREIARTG
                     EGSMRDAQSALDQVISFAGDKIKKEDVEIALGVAGADILKRIIDSIATNKPAEAIAAV
                     DDIVMRGHDLRNFCRDLLAHFRDLLVTKVSGNEELLEAAVCEPAELKRQADLFSEADL
                     VRFFHSLAETETKLRAATQPRYQLEIGIVKLMELRGVESINEILKRLAALETNEPSST
                     TRTPSSSSGSPGKITPERPTTRSAGSSFTAAAAVSPALEVTAPPAELSTIERVKGVLE
                     RQRKMLLIPALEAAQSIVFEESEMCIEFAPDARHFRDTLSKADNNKFVRDACREVTGK
                     EMGVRIVVKDPSADGEPLSHQDEERLEKQKLREMAESNPTVQTMLKTFRGEIVDVRRV
                     DGER"
     gene            6730..6939
                     /locus_tag="VFH96_07605"
     CDS             6730..6939
                     /locus_tag="VFH96_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973874.1"
                     /translation="MRDPVLAGVLSLIIPGVGQLYNGRILAGILWLIVTPGFWLGTGG
                     TLGWICHIVAAYCAYSYAKEHPVRV"
     gene            complement(7008..8942)
                     /locus_tag="VFH96_07610"
     CDS             complement(7008..8942)
                     /locus_tag="VFH96_07610"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HET6973875.1"
                     /translation="MLRKTALLLICLLAPAARAQDAMRGWQWQNPLPQGNSINSIKFA
                     PDKKHGWAIGSNGVVLTTDNGGFEWEEQVSPAQTTLYGLYVKDRSRLVITGARGVVLT
                     TNNGGDKWVLRPSGVRDHLFCVTFAPKNPLLGWAVGTFGSIIATTDGGKTWKTQSSQT
                     SAHLFSITFADAKNGIVAGSHGTLLFTTNGGADWKQSKRLTDFALTGVTFTSEKKATA
                     VGYRGTILNSEDGGESWSTKESLVTTDLHTVFFTDDVHGWAAGENGVVLTTGDGGNIW
                     LPVNVRTSPRLATLYFSDESLGWAAGADGLVVRTDDGGLSWQRLTEGGRDDLANIFFI
                     DNSHGWAVGERGKILFTANGGKSWTIRPSGETVTLNAVTFMTEKSGWVVGNKGTILHS
                     ITNGVTWERVPSPVTEDLFGVSFVSPTEGWVVGARGAVWHTKDEGVTWEFQHTNSLNW
                     LEDVKFVDPLHGIAVGSGGTILRTEDAGLNWKRVDQNLKEWLYALAFADAERGYIVGA
                     RGLVLRTDDGGATWKDLESGINSNLFGVALASRNDVLVVGEQGSIIHSKDAGQTWEIQ
                     PSITSTSLFSIAYRGGTNVWVAGRGGAILRRVDPIATVSLPVTKLPPLLRGAPKLGNA
                     VIDEQDIPKAKPPKKPAEKP"
     gene            complement(8963..9469)
                     /locus_tag="VFH96_07615"
     CDS             complement(8963..9469)
                     /locus_tag="VFH96_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HET6973876.1"
                     /translation="MRDDNQQTSALKQVAIFCDGSSLGNGKEASRAAGVALLGYKGYW
                     RAVGEYLGKATNQQAEIVAAAVGLESLTQPCQVSIFTDSKYVVETMNGRFRRKANLPW
                     WERLDRAAKAHKVHWEWTRGHAGHILQEAADHAARRIAAAGHVEESILREAVDQLGVI
                     ETTDLRSL"
     gene            9543..9866
                     /locus_tag="VFH96_07620"
     CDS             9543..9866
                     /locus_tag="VFH96_07620"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET6973877.1"
                     /translation="MSGLSLKECVPCRGGVPPLNAEEIAKLLPELDGWEVVNNHHLKK
                     SYRFSNFRESQEFVNRVGDLAEEQGHHPDICFGWGNAEISIWTHKIDGLTESDFILAA
                     KIDKL"
     gene            complement(9847..10944)
                     /locus_tag="VFH96_07625"
     CDS             complement(9847..10944)
                     /locus_tag="VFH96_07625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6973878.1"
                     /translation="MKILQISSASSFGGGERYLADLTNALVERGHELYVAVRPNSPIP
                     RHLQLGAHQIFTLPLRNALDVRSAHELERFVRRNGIEIVHAHMARDYSLASYAARRNS
                     KTKFILTRHVLFQLNRLHRHTLARATRVIAVSNAVAEELRASGVVTEKQIAVIPNGID
                     VDRFSRARARFDRVQFLRSMGLPVDCLLVGSIGELRTLKRHDDFIRAAAIVAARFPET
                     QFVLAGLDTSASGEVRKQLEALVNELGLSERFYFLGWLDDAEKLLCAMDVFVSASETE
                     SFGLAIVEAMAAGTAVVATATEGAREVIEDQRTGLLVPIGDIERLAGSVINLLSDPER
                     RRTIATQSAQSAAKNFSLTRMVDEVLTAYRS"
     gene            11068..11589
                     /locus_tag="VFH96_07630"
     CDS             11068..11589
                     /locus_tag="VFH96_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973879.1"
                     /translation="MAKANTVKATLVTALFKSKIAAESAVDALLKRGFNRDDISVLMS
                     DATRSKEFALQTRSHAADGLGIGGAVGGAVGAVLAAIAAVGTTLFLPGINLVIAGPIA
                     AALAGAGAGGATGGLIGALIGAGIPEYRAKVYEAGIKGGGILIGVEARSDEEVERLED
                     LLAELGGEHVRTE"
     gene            11613..11825
                     /locus_tag="VFH96_07635"
     CDS             11613..11825
                     /locus_tag="VFH96_07635"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET6973880.1"
                     /translation="MGLPNKDEVEGKWDQAKGKTKETVGRVLNDDELEAEGQADNAGG
                     QVKEGFGKARRKVGEAIEDVGDAISR"
     gene            complement(11853..13208)
                     /locus_tag="VFH96_07640"
     CDS             complement(11853..13208)
                     /locus_tag="VFH96_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973881.1"
                     /translation="MIWENTKLLLGLLFRPVSSMGRIVDEGHWIYAAVVVAALSMVFH
                     ATVTSVIYNNVEAVYRELPPAPPPDPEDLEAFDEEAVEGFAQPHFVYDRHPLPLVGDR
                     GWWFVSFAPPSFFTAVMGMAVLYIPVLILLVAMSGANASFSVLFRRDYGPLLTCGLMA
                     WAASHLPFTLAGFALEPLHLSEKTALGLWAASAICFGLLMALGLRMLFGINYSKGLLL
                     VAVAALSFSVQAKLFASVSPYLFSPFLLFYGFMMFRGDIGDIGFSLRQRQGFRRSMEA
                     ATINPKDASAHYQLGLIYQYRRQYGEAISRFEKAIQIAHDETDAHFQLGRIAREQGRL
                     QDAINYFSIVLEQDEKHSHSEIWREIGATYLAASMFADAKDALEKFIDRRPYDPEGLY
                     YLGKTLEQLEQRDEAREIFGRCIEAVNTMPSYRYREHRKWDKLAKERLKNPQITQTQS
                     V"
     gene            13207..13407
                     /locus_tag="VFH96_07645"
     CDS             13207..13407
                     /locus_tag="VFH96_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973882.1"
                     /translation="MSANQVSFRLERQQGKTRVGIRHKKCQELFSHKKAQKAQKIFPC
                     TSFVLFVPFCGKIAPDDGATIP"
     gene            13388..14797
                     /gene="fumC"
                     /locus_tag="VFH96_07650"
     CDS             13388..14797
                     /gene="fumC"
                     /locus_tag="VFH96_07650"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET6973883.1"
                     /translation="MVQQSPEQSAKTRIETDTMGAIEVPADRYWGAQTQRSLHHFNIG
                     NDRMPREMIRALGILKKAAALVNEELGKLSPEKAKLIVQACDEVIAGKLDDQFPLRVW
                     QTGSGTQTNMNANEVISNRAIEMAGGVMGSKQPIHPNDDVNMSQSSNDTFPTAMYIAA
                     AEQLNKLIPRIQELHDAIAAKAKQFAGVVKIGRTHLQDATPLTVDQEMGGWADLVARD
                     VERLKLALPGLFDLAIGGTAVGTGLNSHPEFGERAAKKIAELTGLPFKSHPNKFTALS
                     AHDELVEASGSLKTLAASLMKIANDVRWLASGPRSGLGELILPENEPGSSIMPGKVNP
                     TQSEALTMVAVQVFGNDLATSFGGSQGNFELNVYKPVMIHNFLHSVRLLHDATHSFVE
                     FCINGIELNREQIDQHLKHSLMLVTALNPHIGYDKAAQVAKNAHKKKISLRESAIELG
                     YLTGEQFDAMVIPEEMTHP"
     gene            14812..15042
                     /locus_tag="VFH96_07655"
     CDS             14812..15042
                     /locus_tag="VFH96_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6973884.1"
                     /translation="MPDKNDKVKENVYGAFYVDSSCIDCDVCRDTAPENFTRCDENNY
                     SFVFKQPETEEERALCEEALTCCPVEAIGNDG"
     gene            complement(15066..15419)
                     /locus_tag="VFH96_07660"
     CDS             complement(15066..15419)
                     /locus_tag="VFH96_07660"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET6973885.1"
                     /translation="MQSFDTKTDVIIGRVDDLSPGACVSFELPDGNELAVFNVGGEFY
                     ATDNSCPHRGAPLSQGALCGHVIECWLHGWQFDVRSGECLTVPDRIRTYRVTIEDRMI
                     KVNLTTDDADEKSGF"
     gene            15564..16544
                     /locus_tag="VFH96_07665"
     CDS             15564..16544
                     /locus_tag="VFH96_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET6973886.1"
                     /translation="MKRTLLITGVVALFLTATTVLHAKEITVRGKLQKTAEAGGWLIA
                     GRDAKYLILNAKNFQSNGWFKESTNVEAVGETKDVMTTFMEGTPFEAKSMKPVEEGEQ
                     NTATPGRDDNRRVTRVMVAGDSIVQAQPDTAIVTVSVVTQNRQALAAQQDNAAKTDAV
                     VRALKAAAGSGAEVKTSGYSVQPQRVYKEGQPPTITGYEARNSVTVTLSDLTKVGSVI
                     DAAAQAGANDVAGISFTLRQDRQARDRALSEATREAVSKAQVIASALGGRVVRIVEVQ
                     EEGFQQRPPVPVYQYESMQAKSGMVATPVEIGSLDISSRVQVIAEVETNM"
BASE COUNT         3831 a         4638 c         4697 g         3517 t
ORIGIN      
        1 tcgacgtcga tgccgagaaa cacagcgccg ttcaacgtcg ccgcgagtgg ctgtgcgcca
       61 cccattccac ccatgccgcc tgacacgacg agcttgcccg aaagatcgcc ggcaaactgt
      121 ctgtcagcca ttgcagcaaa cgtctcgaac gtgccctgca cgatgccctg cgtgccgata
      181 tagatccaac tgcccgcagt catctgtccg tacatcatca agccgcggcg cgtgctcgtg
      241 agtacgaaac acagcgacgg gtttgcctga ttggaccagc agtgtttcgt cgttttcgag
      301 cgcgcgcagc tcgcgcacga tcgcgtcgaa gctggcccag ttgcgcgcag ccttgcccgc
      361 accgccgtag acgatcagtt catctggacg ctcggcgacg tccggatcga gattgttcat
      421 cagacaacga agcgctgctt cctgatgcca tcctttgcag ctcaactgcg ccccgcgcgg
      481 agctttaatt actcggtggt ccacttaaac tattccatct gattccagag actgcgaaac
      541 cagcgcgcaa tatctgtttt actgagcttt tccatttctc ccgaatcgag ccacacgccg
      601 gcgcactggt cgcacgtgtc gatcggtacg tcttcgataa agatttcttt cagagttccg
      661 ccgtcgcagc gcgggcactt cattgacgag gtgccggctt ctttcgcctg tgctgccacc
      721 cgcatcttct cgcgcagctt ctcgatcgct tcctggttct tgcgatgaaa atattcttct
      781 tcgagtccct tacgacgttc atccattcca ttcgccatgc atgctcctcc agttttagcc
      841 acggattata cggattgaaa atcgccacgg atgatcgctg atgttagctt ttcgatatcc
      901 ggagccatgg tgcggtcctg cgtgagtggc gccacgtgat ggcgaacaat ctcgtacgcc
      961 tgcttcacgc cgcgtcccgg cgcgagcggc gcatgatact ccaacgcctg cgcgcctgcc
     1021 atcaactcga tcgccgtcgc catctcagcc agcttcacga tcgcgcgcat cttcgtcgcc
     1081 gaagtcatgc ccatcgagac atgatcttct ttgccgccgt ccgtcggcac gttatcgatg
     1141 ctcgccggat gtgcgaggat tttcgcttca ttcaaaagcg cgaccgcagt cacttgcagc
     1201 atcatgaatc ccgatgagat tcccggatgc gacgtcagaa acggcggcag tccttcgttt
     1261 gaatcgggat ttactaaacg atcgatacga cgctcagtga tactcatcaa gtctgtcaaa
     1321 gcaatggcgg cgtagtcaaa aacgagggcg agtggcgcgc cgtgaaagtt tccacctgac
     1381 aacacttcac cagtctcgga aaacacgagt ggattgtccg tcgcgctgcc actctcgatc
     1441 tcgacgacct gtttcgcgtg atcgagcgcg gtgcgaatcg cgccgtgcac ttgcggcatg
     1501 caacgcaaac tgtaagcgtc ctgcacgcgc ggatcgccga cgagatgcga ctgccggatc
     1561 tcgctgtctt gcaggaggtc tcgcaagtgc gctgcgactg ctaactggcc cgggtgcgga
     1621 cgcgctgcgt gaatgcgttc gtcgaatgcg actggcgtgc ctttcaaaga ttcgagtgtc
     1681 atcgcgccgg cgacatcagc gagttgaaca agcctctccg cgcgatgaag cgcaagcgcg
     1741 ccgactgcac ccatcgcctg tgttccgttg agcagcgcga gaccctcttt tacttctaat
     1801 tgcagcggtg cgatctgcgc acgctgcaga gccgtggccg cgggcaatcg ttcgccttcg
     1861 tagaaaactt ctccctcgcc gatcgctgcg agcgcgaggt gcgcgagtgg cgcgagatcg
     1921 ccgctggcgc cgacgcttcc tttttcggga atgacgggcg tcacgccttt ctcgagcatc
     1981 gcgatcagcg tgtcgatcac gagcggccgc gcgccgctga atcctgacgc gagtacgttc
     2041 gcgcgcaaca gcagcatcgc gcgtgcttcc ggctccgaca acggatcacc aagaccacac
     2101 gagtgactgc gcacgaggtt cagttgcaac tggatcagat cggcgcgatc gatgctcacg
     2161 tctgacaact tgccaaaacc tgtattgacg ccgtaaacag ttcgaccctg cgtgacgatg
     2221 tcctggacca cctggcgcgc ccgctcgacg cgcacgcgtg cgtcactacc gagaacaacc
     2281 tgttcccggc cctcggcaat gtctacgatt tgctggagtg tgagttgttg accacttagc
     2341 tgaagcatag actaactacg tacctcagct cgcgtctcgg gtgtttcgga aatatcgatc
     2401 ttcttgaact cggcggtttg ttcgcgactg cgcacacgtt ggcgcgcgcg cttcgcgcgg
     2461 actgtggtgc ggcgtgtagt tgcgggtacc caactggtca ttcgtttgaa cggtcgtaat
     2521 gaaaactcgt gtttcggcaa tacttcaccg gtcgcttcgc gcagtcgaac agttccaatc
     2581 gcaaaaccaa atacggcagc gttaaatact atcccgacga cgaagacgat ccaacccggc
     2641 acgatgccaa ccgtagcgcg attgatgaaa acgttgaaat cgcgcgcggg taacggcgag
     2701 agataaactt tcaggttcca gctcaaggcg agcaggacga agatccagag atagttgcga
     2761 cgcaggcgcc gcccgacagc ttcccattcc gtgatcgtaa agtgcggcgt tgtgagatcg
     2821 gctgcgaggt gctcggccca cgactcgtcg gcaggtctgc tgtccggcat caggaggtgc
     2881 gcaaagtaac ccgtttcgag aattctcacg cgactggacc agatttcgta ataacgataa
     2941 cgccgcgctt ccatgaggag gaagatcatg acgagtatcg agttgatcgg gatcacgaaa
     3001 tgcgggttgt taggcgagct gaaaacgaaa gacagcgtcg cgcccgcagt gagcacggcc
     3061 cagtttgtgg tggtgtcgag ccggttgcgc caggtgtttg agcgttgcac ttcaccgcga
     3121 tagaagtgaa tcatcgcggt gttgaattct ccgggcgaca tgcggcgcat cagcgcagcg
     3181 ggtgattgcg ggttgatatc cgtcaatggc tcggctggtg gcgtcgacct gagcctgccg
     3241 gtaaccctat ctgacgttga ttgaccacta cctctatgac ttgcttcggt ggacattgga
     3301 cttgaatgga tcgtgccgcc agagtggggc gcggaactcc ggaaattgat aatagcatag
     3361 aaagacattt agccgcggat tcacgcggat gaacgccgat ctgcgtttat ccgcgtaaat
     3421 ccgcggctaa atgtctttct gtgcttttgt tggcttcctt gacactcttt cagcgtgcgc
     3481 cctatagtgg ccgttcgttg gaatcagagt tggatgagca gtggatgcgg caggcactcg
     3541 tggccgcgcg cgaagcacag gcaatcggtg aagtgcccat cggcgtctgc atcgttagcg
     3601 aaaaccaact cgtcgctgtt tgcggaaatc gcacgcgcac cgactgcgat ccaacagccc
     3661 acgctgaaat cgttgcgctt cgcgaagccg cccgtaagtt cgggaattac cggctcaccg
     3721 gcgtggacgt ttattcgacg attgagccgt gcgcgatgtg cgctggagcg ctgatacaag
     3781 cgcgcgtgag acgtttgatt tacggcgcac cggatgaacg cgccggagca gttatcagtc
     3841 gctttcgtat ctgcgacaca gattttttga atcaccggat tgagatcagc gcgggcgtgc
     3901 tggaagcgga gtgtcgcgcg ctgatgcagg agtttttcca gacgcggaga ggtcgcatag
     3961 tggattagtg caccggtctc gaaaaccgga gtgggtttac gcccaccgtg ggttcgaatc
     4021 ccaccctctc cgccacatga tttcatgtta atcctgagag atcttgtaaa tcttgtctac
     4081 aactgtctcc gcgatcatct ctttacagga tttacaagat ttttcaggat taacatgagg
     4141 agaggtgcag gagtggttga actggcagca ctggaaatgc tgtgaacggg gaaccgttcc
     4201 gtgggttcga atcccaccct ctccgccata aacacttcga ttttcccgac gcccccagcg
     4261 taacaacgat caatccacat tgggggagtc gatgagcgac gacaagttag caatcgaaga
     4321 ggcaaggcgc gcggcgcagc acgaatcagt aaagagtcag gttgaaggcg aggttcaggc
     4381 cgagattggc gagcgcgccg cagtcaagtc agtaccaggc gaatcgcaga gaatcgatca
     4441 agtcgccggt gagtttcgtt cgaaggcagt aaacgaggtc gttgaaagcg agcgtgaagt
     4501 cgagcgctcg cggggcttgg cgcgaatttc ccagatcgtc gattacatct tctgggtgat
     4561 ctacgcgcta ctcggaatgc gttttctgct ggcgctgata gcagcacgaa gcaccgccgg
     4621 gttcgtacag ttcatcgttg caatcagcaa cccgttttac gcgccttttc gcggaattgt
     4681 cgcgagtccg agcacagaca gtggtcacac cttgctcctt ccgatagttg tagcgataat
     4741 cgcctacgcg ctgctacatc tggctatcaa cggattactg cggatgttcg cgcatcgaaa
     4801 gacagagatt taggttatca gatcccgggc tccgcacaac gctcgccatg tgaaccccgc
     4861 caggccggga acggagcaac ggtagcaacg gcagcgtgtg ttgcgggaaa gtccggggtc
     4921 tggccagttc gctactcact tcaaaaataa acgacacaaa tggcctatca agtaatcgct
     4981 cgcaaatggc gtccgcaaac gttcgacgaa gtaacaggcc aggaaccaat cacgcggaca
     5041 ctccgcaacg ccatcgagca tcagcggttg catcacgctt atctcttctc gggcgcacgc
     5101 ggcgttggta aaacaaccag cgcgcggttg ctcgcgaaag cgctcaactg tcacaaagca
     5161 aaagagccga caccgacgcc atgccgttca gacgatccgg atcagtgtcc gtcgtgtcgt
     5221 gaaatcacgg agagtcggtc gatcgacgtg caggagatcg acgctgcgtc aaacacgggc
     5281 gtcgataacg tgcgcgacgc catcatcgcg agcgttggca ttgcacccgc acgcgatcgg
     5341 tacaaagtct ttatcatcga cgaagttcac atgctctccg gcgctgcctt caacgcgttg
     5401 ctgaagacgc tggaagagcc gccaccgcgc gtggtcttca tcatggccac gacggagcgt
     5461 cacaaagttc ccgaaaccat cctttcgcgt tgtcagcagt ttgaatttcg cacgattgcc
     5521 accgcaaaga ttcttgagcg tctcaaactg atcgcgaagt cggaaaagat tacgattcct
     5581 gacgacgcgc tgcgcgagat cgcgcgcacc ggcgagggct cgatgcgcga cgcgcagtct
     5641 gcgctggacc aggtgatcag tttcgcgggc gacaagatca agaaagaaga cgtggagatt
     5701 gcgctcggcg tcgcgggcgc tgacattctc aaacgcatta tcgacagcat cgcgacaaac
     5761 aagccggccg aggcgatcgc ggcggttgat gacatcgtga tgcgtggtca cgacctgcga
     5821 aacttctgtc gtgatctgct cgcacacttc cgcgacctgc tggtgacgaa agtttcaggc
     5881 aacgaagagt tattggaagc ggcggtgtgt gagccggcgg agctgaagcg ccaggcggac
     5941 ttgttttccg aagccgatct ggtgcgcttc tttcactcgc tcgccgagac tgaaactaag
     6001 ctgcgcgctg cgactcagcc gcgttaccag ctcgagatcg ggatcgtcaa actgatggag
     6061 ctgcgtggtg tcgagagtat caacgagatt ctaaaacgac tggctgcact cgaaacaaac
     6121 gagccctcgt cgacaacgag aacgccgtca agttccagcg gcagcccagg taagatcacg
     6181 cctgagcgtc cgactactcg ttcagcagga tcgtcgttta ctgccgctgc cgcagtctca
     6241 cccgcactgg aagtcactgc gccgccggct gagctctcga cgatcgagag agtcaaaggt
     6301 gttttagagc gtcagagaaa gatgttgctg ataccggcgc tcgaagccgc gcagtcgata
     6361 gtgtttgaag agagcgagat gtgtatcgag tttgcgcctg atgcgcgtca cttccgcgat
     6421 acgctttcga aggctgacaa caacaagttc gtgcgtgacg cgtgccgtga agttaccggc
     6481 aaagagatgg gcgtgcggat cgtcgtgaag gatccgtcgg ccgacggcga gccactcagc
     6541 catcaggacg aagagcggct ggaaaaacaa aagctgcgcg agatggcgga gagtaacccg
     6601 actgtccaga ccatgttgaa gaccttccgt ggcgagatcg tcgacgtgcg gcgcgtcgac
     6661 ggcgagcgat aaacgaacaa atcccgcacg gctgtcgtat gagcttccac ttgtggaggg
     6721 cttgaaacga tgcgcgatcc agtgttggca ggagtcttga gtttgattat tccgggtgtt
     6781 ggtcagctct acaacggccg catactcgcc gggatactgt ggttgatcgt gacgcctggt
     6841 ttctggcttg ggacgggcgg cacgctcggg tggatttgcc acatcgtcgc ggcgtattgc
     6901 gcgtattcct acgccaaaga acaccccgtc cgcgtataga ctctaaaaaa attaaccaca
     6961 gataacacgg attaaaaaat cggatctact atccgtgtaa tctgtggcta aggtttctct
     7021 gcgggcttct tcggcggttt tgctttcggt atatcctgtt cgtcaatcac cgcatttccc
     7081 agcttcggcg cacccctcaa taatggcggc aacttcgtca ctggcagact taccgtcgct
     7141 atcggatcga ctcgcctgag aatcgcgcca ccacgaccgg caacccaaac attcgtcccg
     7201 ccgcgatacg cgatcgaaaa caacgacgtg ctcgtgatcg acggctgaat ttcccacgtc
     7261 tgcccggcat ctttgctatg aataatgctg ccctgctcgc ccacgaccag cacgtcattc
     7321 cggctcgcta gcgcaacgcc aaacagatta ctgttgatgc cactctcgag atctttccac
     7381 gtcgcaccac catcatccgt ccgcagcaca agaccacgcg cgcccacgat gtaaccgcgc
     7441 tctgcatcag caaacgcgag cgcgtacaac cactctttca aattctgatc gacgcgcttc
     7501 cagtttagac cagcatcttc cgtgcgcagg atcgtgccgc ccgaacccac cgcaataccg
     7561 tgcagcggat cgacaaactt cacgtcttcg agccagttga gtgaattcgt gtgctgaaac
     7621 tcccaggtca cgccttcgtc cttcgtatgc caaaccgctc cgcgcgcacc gactacccaa
     7681 ccctcggttg gcgacacaaa cgaaacacca aacaagtcct cagtcaccgg actgggaacg
     7741 cgttcccacg tgacgccatt tgtgatcgaa tgaagaatcg tgcccttgtt gccaaccacc
     7801 cagcctgact tctccgtcat aaaagtgact gcattcagcg ttacagtctc gccggaggga
     7861 cggatggtcc aactcttacc accgttggcg gtgaagagaa tttttccacg ctcgccgacg
     7921 gcccaaccat gtgagttgtc gatgaaaaag atgttggcga gatcgtcgcg tccgccttcg
     7981 gtgagtcttt gccaggatag accaccgtcg tcagtgcgca ccacgagtcc gtcagcaccg
     8041 gcggcccaac cgagcgattc atccgagaag taaagcgtcg ccagacgtgg gctggtgcgc
     8101 acattcaccg gcagccagat gttgccgccg tcgccggttg tcaacacgac gccattctcg
     8161 cccgcggccc agccgtgaac gtcgtcagta aagaagaccg tatgaagatc ggtagtgacc
     8221 aggctctctt ttgtggacca cgactcacca ccgtcttctg aatttagaat ggtgccgcga
     8281 taaccgaccg ccgttgcttt cttttctgaa gtgaacgtga cgccggtgag cgcgaagtcc
     8341 gtcagtcttt tcgactgctt ccaatccgcg ccgccgtttg tggtgaagag caacgttccg
     8401 tgtgagcccg ctacgattcc gttctttgcg tcggcgaagg tgattgaaaa caggtgtgct
     8461 gacgtttgac tcgattgagt cttccaggtc ttgccgccat cagtcgtcgc gatgatcgag
     8521 ccgaacgtgc cgacagccca gccgagcaga gggtttttcg gagcgaaagt gacgcaaaac
     8581 agatgatcgc gaacaccact cggtcgcaaa acccatttat caccgccgtt gttcgttgtc
     8641 agtacaacac cgcgcgcgcc ggtgatcacg aggcgcgagc gatccttaac gtaaagaccg
     8701 taaagcgtcg tttgcgccgg actgacctgc tcttcccatt caaaaccgcc gttatccgtc
     8761 gtcaatacaa cgccattgct gccgattgcc cagccgtgtt ttttatcggg agcgaatttg
     8821 atggaattga tggaattgcc ctgcggcaga gggttttgcc actgccagcc acgcatcgca
     8881 tcctgggcgc gggccgcagg cgcgaggagg cagattagta gcaaagcagt ttttctgagc
     8941 atgggctgat ggggaggcgc gattataaac tgcgcaaatc cgtggtttca atgacgccga
     9001 gctgatcaac cgcctcgcgc aggattgatt cttcgacgtg accggctgcg gcaatgcggc
     9061 gcgcggcgtg gtcggcggct tcctgcaaga tgtgaccggc gtgaccgcgc gtccattccc
     9121 aatggacttt gtgcgccttt gctgcgcgat ccagtcgctc ccaccacggc aggttagcct
     9181 ttcttctaaa acgtccgttc atcgtctcaa caacatattt cgaatctgtg aaaatactga
     9241 cctggcaagg ttgagttagc gactcgagtc ctaccgcagc ggcgacgatt tcggcctgct
     9301 ggttcgtcgc cttgcctaaa tactcgccaa cagcgcgcca gtatcctttg tagccgagca
     9361 atgcgactcc agcggcgcga ctcgcttcct ttccgttgcc aagactcgag ccgtcgcaga
     9421 aaatcgcgac ctgcttgagc gcagaagttt gttgattatc gtctcgcaca gttgagattt
     9481 gaatagttgc gacgtagcct atcacgctat cgacggaaat tgtagaatcc ggggaaacgt
     9541 ttatgagtgg actatcgcta aaagaatgtg tgccgtgtcg tggtggcgtg ccgccgctga
     9601 acgcagaaga gatcgcgaag ctcctgcctg agttggatgg ttgggaggtt gttaacaatc
     9661 accatttgaa gaagagttat cgattttcaa actttcgcga gtcgcaggag tttgtgaacc
     9721 gcgttggtga tctcgcggag gagcagggac atcatccgga tatttgtttt ggatggggaa
     9781 atgcggagat ctcgatctgg acgcacaaga tcgatggtct aactgaaagc gatttcatcc
     9841 tcgcggctaa gatcgataag ctgtaagaac ctcatcaacc atccgtgtca ggctgaagtt
     9901 tttagccgca gattgcgcgg attgggtggc gatggtgcgt cgccgctcgg gatcggaaag
     9961 cagatttatc acggatccgg cgaggcgttc gatgtcgccg atcggtacca agagaccggt
    10021 cctctggtct tcaatgactt cacgtgcgcc ttcggttgcg gtggcgacga cggcggttcc
    10081 ggctgccatc gcctcgacga ttgccaatcc gaacgactca gtctctgacg cagaaacaaa
    10141 cacatccatc gcgcacaaca acttctctgc atcatccagc caaccgagaa aataaaaacg
    10201 ttcgcttaga cctaactcgt tcactaaagc ttcgagttgc ttgcgcactt ctccggaagc
    10261 tgatgtatcc agcccggcaa gtacaaactg cgtctccgga aaacgcgcag caacgatcgc
    10321 ggccgcgcga ataaagtcgt cgtgacgctt caacgtcctt aactcaccga tactcccaac
    10381 cagcaaacaa tcaaccggca atcccatgct gcgcagaaac tgcacgcgat cgaaacgcgc
    10441 gcgcgcgcga ctaaaccgat cgacatcgat cccattcgga atgactgcga tctgtttttc
    10501 cgtaacaacg cccgacgcgc gcagctcctc cgcgaccgca ttcgaaactg caatcacacg
    10561 tgttgctcgc gcgagcgtgt gacgatgcag tcgattgagt tgaaacaaaa cgtgacgcgt
    10621 gaggatgaac ttagtcttcg aattgcgacg cgcggcgtac gaagcgagtg agtaatcgcg
    10681 cgccatgtgg gcatggacga tctcgattcc gttgcgccgc acaaacctct cgagctcatg
    10741 cgcgctgcgc acgtcgagag catttcgcaa cggcagggta aaaatttggt gagcacctaa
    10801 ctgcaagtgt cgcggtatcg gcgagtttgg acgcactgcg acgtacaact cgtgtccgcg
    10861 ttcaacaagt gcatttgtga gatcagcaag ataacgctcg ccgccgccga aagacgaagc
    10921 tgaactgatc tgaaggatct tcaatctgaa tgtccgtacg aaagccgtcc acagattacg
    10981 tgcgatcaca cagaaatctg cgcaatctat gatggcatac gtgttgccaa ccgagatgtc
    11041 aaatagaact gtctgttgga ggattgtatg gccaaggcaa atactgtaaa ggcaacatta
    11101 gtaacagccc tttttaagag caaaatagcc gccgagtcgg ccgtcgatgc gctcctgaag
    11161 cgcggcttca accgcgacga catcagcgta ctcatgtctg acgccactcg atccaaggag
    11221 tttgctctgc aaacacggtc gcatgctgct gacggtttgg gaatcggggg cgccgttggt
    11281 ggagcagtag gcgccgttct cgcggcgatt gccgcagtcg gaacaacgtt gtttcttccc
    11341 ggaatcaatc tcgtgattgc tggtccaatc gctgcggcac tcgccggcgc aggcgcgggc
    11401 ggagcgacgg gcggactgat cggcgcattg attggagcag gcattcctga atatcgcgcc
    11461 aaagtttacg aggccggtat caagggcggt ggaatcttga tcggtgtgga agcacggagt
    11521 gacgaagaag tggagcgcct cgaagatttg ctggctgagc tcggaggcga gcacgttcgc
    11581 acggaataac taatacataa ggagggttag caatgggttt accgaataag gatgaagttg
    11641 aaggaaaatg ggatcaggct aaaggcaaaa cgaaagagac tgtcggcagg gttctcaacg
    11701 acgacgagct cgaagccgag ggccaggcag ataatgccgg tggtcaggtg aaggaaggct
    11761 tcggcaaagc acgacggaag gttggcgaag cgatcgaaga cgttggcgac gcgatcagtc
    11821 gttaacgtcc acagattaca cagatccaca gattacacag attgagtctg cgtaatctgt
    11881 ggattcttaa gtctttcttt cgcgagtttg tcccacttgc gatgctctcg ataacgatac
    11941 gagggcattg tatttactgc ctcaatacaa cgaccaaaaa tttcgcgtgc ttcatctcgt
    12001 tgctcgagtt gttcgagcgt cttgcctaag taatagagtc cttcgggatc gtaaggacgc
    12061 cgatcgatga acttctcgag cgcgtctttg gcatcggcaa acatcgacgc cgcaaggtac
    12121 gtcgcaccaa tctcacgcca gatctcgctg tgcgaatgct tttcgtcctg ttcgagcacg
    12181 atactgaaat agttgatcgc gtcttgcagt cgtccttgtt cgcgtgcgat gcggccgagt
    12241 tgaaagtgcg cgtcggtttc gtcatgggcg atttgtattg ctttctcgaa acgcgagatc
    12301 gcttctccgt actggcggcg atactggtag atcagaccga gttgataatg cgcgctcgca
    12361 tccttcgggt tgatcgtcgc ggcttccata gagcgacgaa atccctggcg ctgtcgtaaa
    12421 gagaaaccga tatcgccgat gtcaccgcga aacatcatga aaccgtagaa aagcaggaac
    12481 ggcgagaaca ggtatggcga gacgctggcg aacagttttg cctgtactga aaacgagagc
    12541 gccgcgaccg cgaccagcag caagcctttt gaataattaa taccgaagag catgcgcaac
    12601 ccgagcgcca tgagtagtcc gaaacagatt gcgctggcgg cccagagtcc gagcgccgtc
    12661 ttctcactca aatgcaacgg ttcgagggca aatccggcaa gtgtgaaagg caagtgagac
    12721 gctgcccaag ccatcaatcc gcacgtcagt agtgggccgt aatcacgccg aaacagcacg
    12781 ctgaaactcg cattcgcgcc tgacatcgcg accagaagaa tcaataccgg aatgtacaag
    12841 accgccatgc ccatcacggc ggtgaagaaa cttggcggcg caaatgagac aaaccaccag
    12901 ccacgatctc cgacgagcgg caatgggtgt cgatcgtaga caaagtgcgg ttgcgcgaac
    12961 ccttcgacag cttcctcgtc gaaagcttcc aaatcttcgg gatccggagg gggcgccggt
    13021 ggcagttcgc gataaaccgc ctcgacgttg ttgtaaatga ccgaagtgac ggtcgcatga
    13081 aagaccatcg agagcgcagc cacgaccaca gcggcataaa tccagtgacc ttcatcgaca
    13141 atcctgccca tcgacgacac gggacgaaaa agtaggccaa ggaggagttt tgtattttcc
    13201 caaatcatga gtgctaacca ggtgagcttc aggctggaac gccaacaggg caaaaccaga
    13261 gtagggattc gccacaaaaa gtgtcaagaa ctttttagcc acaaaaaggc acaaaaagca
    13321 caaaagattt tcccctgcac ttcatttgtg ctttttgtgc ctttttgtgg caagatcgct
    13381 cccgacgatg gtgcaacaat cccctgaaca atccgcgaaa accagaatag aaactgacac
    13441 gatgggtgca atcgaggtcc ccgccgatcg ctactgggga gctcagacgc agcgctcgct
    13501 tcatcacttc aatatcggca acgaccgcat gccacgcgag atgattcgcg cgctcggcat
    13561 tctcaagaag gccgccgccc tcgtgaacga agagctcggc aaactttcgc ccgagaaagc
    13621 aaagctgatc gtgcaggcgt gtgacgaagt tatcgcaggc aagctcgacg atcagtttcc
    13681 tctgcgcgtc tggcagacgg gtagcggcac gcaaaccaac atgaacgcca acgaagtgat
    13741 ctcgaatcgc gcgatcgaga tggccggcgg cgtgatggga agcaagcagc cgattcaccc
    13801 gaacgacgat gtcaacatgt cgcagtcgtc aaacgacacg tttccgaccg cgatgtacat
    13861 tgccgcagcc gagcagttga acaaactcat tccgcggatt caggagctgc acgacgcaat
    13921 tgctgcgaag gcgaaacagt ttgccggcgt cgtaaagatc ggacgaacgc acctgcagga
    13981 tgcaacgccg ctcacagtcg atcaggaaat gggcggctgg gcagatctcg tcgcccgtga
    14041 cgtcgagcgc ttgaaactcg cgctgcctgg attattcgat ctcgctatcg gtggcacggc
    14101 cgtcggcacc ggactcaact cgcatcctga attcggcgag cgagccgcga agaaaatcgc
    14161 agagctcacg ggcttgccgt ttaagtctca tccgaacaaa ttcaccgcgt tgtcagcaca
    14221 cgacgagctc gtcgaagcga gtggctcgtt gaaaactctc gctgcttcgc tgatgaagat
    14281 tgcgaacgac gtgcgctggc tggcgtcagg gccgcgttcc ggtttaggtg agctcatcct
    14341 gccggaaaac gagccgggct cgtcgatcat gccgggcaaa gttaatccga cgcagagcga
    14401 agcgctcacg atggtcgcgg tgcaggtttt tggtaacgat ctggcgacga gttttggtgg
    14461 ctcgcagggc aatttcgagt tgaacgttta caagcccgtg atgatccata acttcctgca
    14521 ctcagttaga ttgctgcacg acgcgacgca cagtttcgtt gagttttgta tcaatggaat
    14581 cgagctcaat cgcgaacaga tcgatcagca tttgaaacac tccctgatgc tcgtgaccgc
    14641 cttgaaccct cacatcggtt acgacaaagc tgcccaggtt gcaaagaacg ctcacaagaa
    14701 aaagatcagt ttgcgtgaat ccgccatcga gctcggttat ttgaccgggg agcagtttga
    14761 cgcgatggtg attccggaag agatgacgca tccctgacct gcgaatccag cgtgcctgat
    14821 aagaacgaca aagtgaaaga gaatgtctac ggtgcgtttt acgtggactc ctcgtgcatc
    14881 gattgcgatg tctgtcgcga caccgcgccg gagaacttca cgcgctgcga tgagaataat
    14941 tattcgttcg tctttaaaca gccggagact gaggaagaac gggcgctttg tgaagaggcg
    15001 ctgacttgtt gtccggttga ggcgataggc aacgacggat aagtttagcc acagattaca
    15061 cggatttaaa atccggattt ttcatccgcg tcatccgtgg ttaagttgac cttgatcatc
    15121 cgatcctcga tggtcactcg atacgtccgg atccgatccg gtacagtcaa acactcacca
    15181 ctccttacat caaactgcca cccgtgcaac caacactcaa tcacatgccc gcaaagcgca
    15241 ccctgactta acggcgcgcc gcgatgcggg caggagttgt cggtggcata gaattcgcca
    15301 cccacgttga atacggctag ctcgttgcca tcgggcagct caaaactaac gcaggcaccc
    15361 gggctcaggt cgtcaacgcg gccgataatt acgtctgtct tagtatcgaa tgattgcatt
    15421 gaatggatgg taacaaaaca cgttttgcag aacctaattc acaagttgcc agcaagtttt
    15481 cgtttcctgc gcgttctcta tcttcgccgc cggtttccac tctccctcgg ctgtgagatt
    15541 ctaggaccag aagaggagag atcatgaaac gaacactttt aattactggc gttgtggcgc
    15601 tttttctcac tgccaccacg gtgctgcacg ctaaagaaat caccgtacgc ggaaaactac
    15661 aaaaaactgc cgaggcgggc ggctggctca tcgctggccg tgacgcgaaa tatctaatcc
    15721 tgaacgcaaa aaactttcag agtaatggtt ggtttaaaga gtcgacgaat gtcgaggcgg
    15781 tcggcgaaac caaagacgtg atgacgactt tcatggaagg cacgccgttc gaagcgaagt
    15841 cgatgaaacc ggtggaggaa ggggagcaga ataccgccac gccgggtcgc gacgacaatc
    15901 gccgtgtgac gcgcgtgatg gtcgccggcg attcgatcgt ccaggcacaa ccagacactg
    15961 cgatcgtgac cgtttcagta gtgacgcaaa acaggcaggc actcgccgcg caacaagaca
    16021 acgctgcgaa gactgacgct gtcgtgcgtg cgttgaaagc ggcagcagga agtggcgcgg
    16081 aagtgaagac gagtggttac agtgtgcagc cgcaacgcgt ttacaaagag ggtcagccac
    16141 cgacaatcac cggttacgaa gcgcgcaaca gtgtgaccgt aacgctgagc gatttgacga
    16201 aggtcgggag cgtgattgac gcagccgcac aagccggcgc gaacgatgtc gccggtatct
    16261 cgtttacctt gcgacaggac cggcaggcgc gtgaccgcgc attgagtgaa gcgactcgtg
    16321 aagcggtgag caaggcgcag gtgatcgcgt cggcgctcgg cgggcgtgtg gtgcggattg
    16381 ttgaagttca ggaagaaggg tttcagcaga gaccgccagt gccggtttat cagtatgaat
    16441 cgatgcaggc gaaatcagga atggttgcga cgccggtcga gattggatca cttgatatca
    16501 gttcgcgagt ccaggtgatc gctgaggtcg aaacgaatat gtaacgcgga tcttacggat
    16561 cttaaagacg attagtatgg atttcccccc ccagccccca gcaagatgag gttcctggtt
    16621 aaggagggaa ggcaatggcc agatacagaa acgagatgga tgacgatcgg gactattacc
    16681 cac
//
LOCUS       DASSFQ010000212        30886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1129505, whole genome shotgun sequence.
ACCESSION   DASSFQ010000212 DASSFQ010000000
VERSION     DASSFQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 30886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30886
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129505"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFH96_07670"
     CDS             <1..747
                     /locus_tag="VFH96_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET6973887.1"
                     /translation="ARTLSLSGIVGAQGSDSIWYVLYQPAGFLLFLISGIAENNRAPF
                     DLPEAESELVAGFHTEYSGMRWSLFFMAEYAAMVVVAAVATTVYLGGWYFPFVYRLET
                     AGHHNLFVLVSVLVFLVKASLILYFYFWLRWTLPRFRYDQLMDIGWKWLIPSALINIV
                     ISAFAIFLVQALNGFRGMQTIQSLDRGLNLTGSGKAIMIVMGLVGLFATAALLSRINW
                     RSRDFNLKVQRRDIKLVNMPKGKPAVQPGN"
     gene            750..1283
                     /locus_tag="VFH96_07675"
     CDS             750..1283
                     /locus_tag="VFH96_07675"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET6973888.1"
                     /translation="MPLVKTPKPTLGQKLKRFFMIDLIKGLGLTLKYNLGAFTDKDAV
                     AGKGIYTEQYPKVRPDVAPRFHGAPRLNMDPETHDTLCIACNLCAIACPEDCIDVIPM
                     DVEIVVAGKPRRKKVLDEFIFDTSRCMFCALCQEACPTSCLELTQDFELATYSRAGFV
                     WNREMLEQGRETVKYTS"
     gene            1399..2037
                     /locus_tag="VFH96_07680"
     CDS             1399..2037
                     /locus_tag="VFH96_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET6973889.1"
                     /translation="MTLSGYEALFFWIFGLAAIFAGLLTVMARNAVHSALFLISSLVS
                     VAALFILARAEFIAGVQILVYVGGVMVLFLFVIMLVNVGAEERGTEEIFNNQKQVTAS
                     LIFCLLLVGGMLYAINTGYRGLQVRYPATPDAVAAQESIRDEAHAPSATGSSRITKDT
                     ESVGSSLYRYASLPFEIASILLLVAIIGSVMLARTLKQEASADDVDPDFESR"
     gene            2072..2470
                     /gene="nuoK"
                     /locus_tag="VFH96_07685"
     CDS             2072..2470
                     /gene="nuoK"
                     /locus_tag="VFH96_07685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET6973890.1"
                     /translation="MLSFFNILLLLQETETRVDLMKRAAVNPDLSKFLVIGALLFIIG
                     VAGVLTRRNIIVIFMSIELILNAANLNFIAFSRYLSAMGNVNAVAGQIFTVFVIVVAA
                     AEAAIGLGIVIALYRNKETIWVDEIDLLKW"
     gene            2522..4987
                     /gene="nuoL"
                     /locus_tag="VFH96_07690"
     CDS             2522..4987
                     /gene="nuoL"
                     /locus_tag="VFH96_07690"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET6973891.1"
                     /translation="MFKYIWLVPLLPLIGAAINGLLGRKLRFSEQIIGGIAVGSVALA
                     FLISVAAVYSYGFGGHAEWPKPYLTSQDGFSFDWIPGGAVSVNHGAQYALLNIEWSYQ
                     LDPLSSIFMLIVTGVGLCIFIFATGYMHGETGFYRFFAYMGLFMFAMLVLVMGSNFLM
                     MFVGWEGVGLCSYLLIGYYFDRQEAANASRKAFITNRVGDFGFALAIFGIIATFGSAQ
                     YVNVFDQAKSYPIELIGHWGVLSWIALGLFIGACGKSAQLPLHVWLPDAMAGPTPVSA
                     LIHAATMVTAGLYMVTRTNVIFQHSQTMLLVVALVGAATAIFAATIGITQNDIKKVLA
                     YSTVSQLGFMFMACGVGAFTIGIFHVMTHAFFKALMFLGAGSVIHGMHHEQDMRRMGG
                     LKKYMPYTYWTFMAGWLAICGVIPFSGFWSKDEILWNAANTTHIPLGWIVWLVGTIAA
                     TCTAFYMTRLMAMTFWGRERFLEAPAGGEADEAHASAYDKGLTQHDATFASAGDRPRH
                     EPGEHVGPVHEDVAHHIASHGHGGHHGAHIPHESPASMWVPLAVLAILATLGGLVGIG
                     PAFRTLTGTEHPGGHLNIVTWLNPIIWNPVTREFNTPEGGHEAAAPAETAHAETAAPP
                     VAEGAAEAEHGSAAAVYGDTGFNLAHAVEGSTHSELATEWIFILISLVVAGIGMLLGG
                     LFYVWRPALANVWSQRLRPLYVASYNKYWVDEFYGLTVTRRTMDLARAVFRFDSRIVD
                     GIVNGLASLSRLCSRIVGGFDKYFVDGLVNTVAAFIMRLMSPLFRAAQTGFTQNYALV
                     MVLGLMVAVAVFFARDILAVFGR"
     gene            5024..6709
                     /locus_tag="VFH96_07695"
     CDS             5024..6709
                     /locus_tag="VFH96_07695"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET6973892.1"
                     /translation="MTNSYILSLITWLPAVGAVLILALMKKDQSSLIKRFATLWFALD
                     FIISLFLLKYNRGGGGMQFLEDHQWIPVIGARYQMGVDGVAVLLIVLTTLLGAIAALS
                     SWNYIQKREKEYYVLLLLLQTAVIGVFASMDLFLFYLFFEVSLVPMYFLIGIWGGENR
                     LYAAIKFFLYTLVGSVVMLLGVLKMYFLTQDTAMTGKITQATINLLAGNNEAAQLVSS
                     TLQAAVVNGTGTFNIVYMQAMGSVLPTGTLQVLLFAAFALGFAIKVPMFPFHTWLPDA
                     HVEAPTAGSVILAGILLKLGTYGFYRFNLPMFPWASKDQSYFGSFGVRSVVVFLAIVS
                     IIYGALAAMYFVVKKDGDVKKLVAYSSVSSMGMVMLGLFALNPNGVNGGVLQMINHGI
                     YSGALFLLVGIIYERRHTRNVAEFGGLSHVMPGYAAIFLGMVMTAIGLPLLAVFISEF
                     LALRGAFEANPYWAAWAALGIILNAGYFLWLYQRMFFGTIDNPKNEKLTDLSGREWAY
                     MVPLMILALWIGVYPKPFLEYIQKPVNALVQQVRPDYVIPGQAAPARTERAER"
     gene            6776..8320
                     /locus_tag="VFH96_07700"
     CDS             6776..8320
                     /locus_tag="VFH96_07700"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET6973893.1"
                     /translation="MFLLQANTTSLINVSDLRLIAPELILTVSACVALVMEVILPYRK
                     SKLTAYFALAGIALAFVSLALQMTGPMNVSGFYGMVRIDGFAILFRIIFLISAALAIG
                     ISTRFLDIEGEQHGEYYSLILFATVGMMFIACGYDLISLYISLELMALTFYVLVAFTK
                     REKKSNEAAMKYFLLGAFSSGVLLYGMSLIYGLAGSTNVGEIGSNISEIVRNIQAATA
                     DPTTNQQIIQLRPLLLLGMIALSAGLFFKIAAVPFHMWAPDVYEGAPTSVTAFLSTGS
                     KAASFALYARIFSEALPAMRADWAPLLGLVAAITIMVGNWAAVTQENSKRLLAYSSIS
                     NAGYLLLALIANNEYGRIGLIVYLFVYTLMNMGAFGVIISLRRRGIIGDNVDDLTGLA
                     HKAPVMAAMMAIFMLSLGGLPVTGGFIGKYFLFGGLIQQGAIEHKSWYYWLAGWAIIN
                     TVVSFAYYFRFIKVMYLGDRIADNKPLALSPALQVALAIAVIGIIIIGVYPQPFIALA
                     KTLMPS"
     gene            8336..9502
                     /locus_tag="VFH96_07705"
     CDS             8336..9502
                     /locus_tag="VFH96_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6973894.1"
                     /translation="MVVYYFLAVVSCWFGLQSLLSGIRYAAYVRRETSRPLPDFQPFV
                     SVIAPGRGLEPGLIDNLRPLVAQDYARYEVLFVFDALDDPAIKIVDELKNAATRTIIA
                     GPATDSGQKVHNLRVAVNEVDPQSQVLVFVDTDARPANDWLRKLVAPLADESLGASTG
                     YRWFIPDRGGIASRLRSVWNASVASALGGNTAKNFCWGGSTAIRRTTFTKLDVTERWR
                     GSVSDDFTITHVLKQANLPIHFTPNCLVASVGDCDFRELIEFTTRQIKITRVYASHLW
                     LPLLLGSSLFAIAFFGGLILIGVHPRSSAAYIFPLIIFALGAAKSFIRFRAVSTVLKT
                     SRLDLVAHILLWPIASLLYLYNAVVAGFSRRIKWRGITYELKSPTEAVIISRES"
     gene            9502..10623
                     /locus_tag="VFH96_07710"
     CDS             9502..10623
                     /locus_tag="VFH96_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6973895.1"
                     /translation="MRNKAAQFVQLLRSTKREKRLGTVILFVTSRCNAFCQTCFYHEE
                     LNQPGDMTFAQIEKISRTMPPITDLWLSGGEPTLRRDLSDVIDLFVKNNGVDRIIIPT
                     NGIIKTRVYDVVDRALGNNPQIDLYLNIALDGYGQTHDRIRGVPGNWEKALACIESLY
                     ALKEMYTDRFRLNVNTVVCAENYTEIELLSEFMWSNYRLDGQYFNIIRGETLVGETIK
                     QVPPEVLPAMYAHVSKLTKRYGERMFAADDASKRFVKNAVYAGTIITHYRTQHANFQQ
                     PTAWTFPCTAGQTTAVIDYNGDVRACELREKFASLADFDFDFNALWSANDRQTELTAI
                     DGGKACWCTHVCFIHDSMRHSRRAMLYNVPKNYLTRESW"
     gene            10620..10835
                     /locus_tag="VFH96_07715"
     CDS             10620..10835
                     /locus_tag="VFH96_07715"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET6973896.1"
                     /translation="MIKKEDQDENRKAGFAYAAGIALFASVVTFSAAGWFVDKWFGIA
                     PWGIIGGIVLGSAAGLFEFVRISSKTY"
     gene            10917..11294
                     /locus_tag="VFH96_07720"
     CDS             10917..11294
                     /locus_tag="VFH96_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HET6973897.1"
                     /translation="MLESRVFRTMAIASALSVSVAAFIAPWRVTTGLLLGGLLALFSH
                     RWLSNSASAAVQLSATGGRSQWKLAQFVLRYVVIAAAVFAAYTLDLVSLTAVLAGMCS
                     FVVAMFVEAIREFYFAIIKREEN"
     gene            11298..12230
                     /gene="atpB"
                     /locus_tag="VFH96_07725"
     CDS             11298..12230
                     /gene="atpB"
                     /locus_tag="VFH96_07725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET6973898.1"
                     /translation="MFWFAEAAEAAHGAAEHAEEAEHHTPIIVELVNHYFGEYAYRFE
                     IAYTKPFWDKFLGFFGTTPEDAFGPYTPENAIPWYTVMFVIACILSVVIIWILKGKLS
                     ESKPGHGQQTLEVSVLAVRSMIEDIIGPHGLKYFPVVMTFAVLILVSNLMGLFPLFMS
                     PTSATSVTFALGLSSFLYYNYIGIAENGLVGHLKHLAGPIWWIAPLIFPIELISNLIR
                     PFSLGIRLFGNLFADEKVLETLAGLHPWQSWFAPILLMPLSVFVALIQTFVFILLSQL
                     YISEVSHAPHHHEHGADDEEDEYDGDEYIAPVLT"
     gene            12455..12793
                     /locus_tag="VFH96_07730"
     CDS             12455..12793
                     /locus_tag="VFH96_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET6973899.1"
                     /translation="MSKFRMLLFAVIGILASSMSVFAQAGAEAGDNVYSVNKYRAIAA
                     GIGFAIAVFGGALGQSRIGAAACEGAARNPGAAGRIQTMMILGLALIESLVLFALLVI
                     FAVVKGVVTP"
     gene            12908..14512
                     /locus_tag="VFH96_07735"
     CDS             12908..14512
                     /locus_tag="VFH96_07735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6973900.1"
                     /translation="MKTCPKCGVEYPDTTTLCPADGVALEKDPDSLVGTTLAGKYRID
                     ARLNEGGMGTVYRGCHVLMDKTVAIKVLRPSLAADEKIVARFSREARAASRITHPNAI
                     SVTDFGEDERGHVFLVMEYLSGKTLKHVIREEGPLPLARVVEITRQIGDALNAAHQQG
                     IVHRDLKSDNIMLVDTMTGDHAKVLDFGIAKINEPDGVVDTNLTAPNLVIGTPQYMSP
                     EQCSQDVEIDARSDIYSLGVILFEMLVGHVPFSGDSPTIVMMKHLQDPVPSVLEERSD
                     LPASVARVVARAMAKVRDNRYQNVAELIEDLTIASGMTIHRLGPVGVTSTVPTPDPDE
                     VTVVRAREVMPPVSPVPPVQRREPVVVTVSGQMPPPAPAAAGFNPLKVLIPSVIGLLV
                     VFAAIYALTRNTTPGEANTNQQVVPSLAADPNSQPVQPAQPATGKGEEGIPSGGSITP
                     PANASPSASVSASPAPTEDFTPDANANSNTNTNSNQNSNGNSNRKAPALPEPTRSVVP
                     DNPPPPVPSATKPPAEKPSPTATPMK"
     gene            complement(14524..15555)
                     /gene="lpxK"
                     /locus_tag="VFH96_07740"
     CDS             complement(14524..15555)
                     /gene="lpxK"
                     /locus_tag="VFH96_07740"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET6973901.1"
                     /translation="MKSIVLPPLSLLYGAVTRTRLSLYRRGTFQTTKLDRPVISIGNI
                     TTGGTGKTPLVEYVARTIASQGKKVCILTRGYGRKDPHLQVIVSDGYGVLASPSEAGD
                     EPYLLATRLTGQAAVISSADRIAAGQEAIKDFGTEVFVLDDGFQHLRLARDLNIVCID
                     ATNPWGGGRLLPYGRLRESLDGLTRADCFVLTRCDQVESVEALRAEIVELTGGRPIFE
                     SRMRPLRVTSLKNGPETIALPGRVGAFCAVGNPSSFFESLKRLGYELGVERAFADHHA
                     YSQSDVDALDQLARQTGASALITTAKDAVKLKGMEFSRPCYVLEIEIAIDDADGFIRR
                     LRRVFETSV"
     gene            complement(15552..16853)
                     /locus_tag="VFH96_07745"
     CDS             complement(15552..16853)
                     /locus_tag="VFH96_07745"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HET6973902.1"
                     /translation="MYLAYSLLLSLGLIVLIPHFLFQALAHGKYIAGLRQRLGSVPPV
                     TGKPVIWLHCVSVGETQAARPLAKRLKQQFPHHALVVSTITLTGQNLARDVFRNQAES
                     VFYFPFDWRWTVRRALKAVNPAVVLIMETELWPNFLRECNARRIPVALVNGRISRQSF
                     RRYRLIKFFLRRVLSSLSIAVMQSETDLERLKALGMASDRLFNAGNLKFDADLSSELT
                     SKTEEIRHRFAFDSNRPLILAASTHAPEEQIILESIQKLRQKQPARLMLAPRHPERFN
                     EVAGLLQKSGLSWTRRTNPPNANDADASVILLDTIGELPATYALAQIVFVGGSIVDKG
                     GHNVLEPAAAGATVVTGAHTHNFHAIVELMNEAGALVQLPALEDAAASDELAYVFEKL
                     LASPAERDELGRRAKELITANQGAADRTMQLIAPPILAATS"
     gene            complement(16861..17424)
                     /gene="efp"
                     /locus_tag="VFH96_07750"
     CDS             complement(16861..17424)
                     /gene="efp"
                     /locus_tag="VFH96_07750"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET6973903.1"
                     /translation="MAIPATQIRRGMVIVFEGQPCKVLEFRHHTPGNLRAMIQTKLRN
                     IRTGASFEHRFRSADTVDRASLEQHEMEYLYSDGSHHHFMNTENYEQTALSEEELGDA
                     APWLMPGMRIQAEFYEGAPIGIDLPPSMELTVTQTEPQLKSATVSNVNKPATLENGVT
                     ITVPPFINEGDRIRVDPSEGRYIERAK"
     gene            complement(17695..18414)
                     /locus_tag="VFH96_07755"
     CDS             complement(17695..18414)
                     /locus_tag="VFH96_07755"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HET6973904.1"
                     /translation="MNEVRKYFADAFRHMGGNRGVPAIEVRFYPYAGLRHTIRLRSGR
                     VYVRLSDICKDSPPEVLRALAWILVARLLGKRVPSIHDRVYRDYSLTPSVMRSSDLAR
                     RGRGRKMISTAQGEVYDLDRMFAKLNRRYFDGAIPKPTITWSQRRTRSILGHHDHIYD
                     SITISKTLDSKDIPEWFAEYIMYHEMLHIIHPARLINGRRYYHTSAFRQDERKFPYYE
                     ESQKWLERLARLRRVPRARAA"
     gene            18609..19721
                     /gene="hrcA"
                     /locus_tag="VFH96_07760"
     CDS             18609..19721
                     /gene="hrcA"
                     /locus_tag="VFH96_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET6973905.1"
                     /translation="MPRRASNLQTTETAGLDSRAEVLLAALIKEHLVTGEAVGSRVLS
                     DRFSHGFGWSPATIRNVMADLEESGFVEQPHTSAGRVPTDKGYRYYVDNMLGEARLSR
                     TDLKAIDHLFESIDAGSSPDRLMETASHVLSALSQNVGIVVSPSLADNRLNHIEFMLL
                     PDHRILVVLVSTANIIHNKVIRVAGDDLTQDDLEHTARYLNTEFSGKSLLAIRTEIVE
                     LMKEEKALYDRLLQNAILLCERSLEGEDTSAGVYVDGTSNMLTKPEFVDIERMRELFR
                     TFEEKSRLIKILNECVHDAAGYPGDVHVIIGREHPASSMRDCTLITAPYRVGSNDNIG
                     SLSVVGPMRIEYARIMAMVNYMARLIEHRLGEQTAR"
     gene            19743..20417
                     /locus_tag="VFH96_07765"
     CDS             19743..20417
                     /locus_tag="VFH96_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6973906.1"
                     /translation="MTSDRRDKGSHRIPIKFTDADDMAAEDPNYEGEEIDLSDESLNE
                     EDLFSAGDVEPEGGGPEKAELIATRAELKRIEAENAELKDLVARRQADFENYRKRVER
                     ERSETYNRVVADVAAKLLPVVDNLKRALEAEASVEAAESDEFRHFLSGVDLINKQLNG
                     VLDALGIKPVAAVGEQFNPHVHEAVVTEPTDDYAPDTVMQELVTGYRLGDKLIRPALV
                     KVATRK"
     gene            20524..22347
                     /gene="dnaK"
                     /locus_tag="VFH96_07770"
     CDS             20524..22347
                     /gene="dnaK"
                     /locus_tag="VFH96_07770"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6973907.1"
                     /translation="MGKSIGIDLGTTNCCVAVLEGGTVQIVPNKEGGRTTPTVVGFTE
                     KGERLVGQIAKRQAVTNAANTVHAVKRLIGRKFSSREVSKMRESAPFEIVDSPNGDAR
                     VRVQGRNYSPPELQGIVLQRLKAAAEEFLGESVTDAIITVPAYFDDTQRQATKDAGKI
                     AGLKIERIINEPTAAALAYGLGKSSVERIAVYDLGGGTFDISILEMNDGVFEVLSTSG
                     NTFLGGEDFDERIMSWMAGQFLSDTGIDLREDRLALQRLKEVAERAKCDLSTTNETVI
                     NLPFIAADATGPKHFNKTLTREQFETLVSDLVEQTIEPCQTALKDAKLQASDIHKVIL
                     VGGQTRSPIVERTVEKIFGRKPSSEINPDEVVGMGAAIQGGVLNGDVKDIVLLDVLPL
                     SLGLATRGGLFTKIIERNSTIPLRESRTFTTVVDNQSSVEIHVLQGEREVADGNRSLG
                     KFELVGIPPNPRGVPQIEVSFEVDANGIVSVSAQDKATGREQQMRITPTSGLNPEEID
                     RLIHEAESFAETDKNTKEVLVLRTRLDSLLRNTQKSFTKFGGLLPEIDQESADKAFTE
                     AEAALKSDKMDEIKIAMNKVERVAGQLTSAMLDPEADAVES"
     gene            22427..23548
                     /gene="dnaJ"
                     /locus_tag="VFH96_07775"
     CDS             22427..23548
                     /gene="dnaJ"
                     /locus_tag="VFH96_07775"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET6973908.1"
                     /translation="MSKRDYYEVLAVSRTATDQEIKSAYRRLAVQYHPDKNPNDATAE
                     EKFKEAAEAYSVLSNPEQRKRYDRFGHAGVSSGAAGAGAWGAPGFGGIEDILGDLFGF
                     GDVFGGRGGQRRSSAQRGADLRYDLEITLEEAANGMTAQLRIPRLESCDTCKGSGAAA
                     GSHAENCTTCAGTGQVRYQQGFFSVARTCHVCRGTGKVIKNPCPDCSGTGRVEREKQL
                     EVKVPAGVETGSRLRVTGEGEAGAQGGATGDLYVVIHVAEHDMFERQGSNLYEAIPIT
                     FAQAALGAEVMVKTLIDKEEKLKIPMGTQTGTVFRLRGKGMPALGGRGRGDLFVSVTL
                     ITPTTLTREQRRLLEQLAEVENKDLESKGLVDKVRDIFG"
     gene            23599..24747
                     /locus_tag="VFH96_07780"
     CDS             23599..24747
                     /locus_tag="VFH96_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HET6973909.1"
                     /translation="MLVRPRHFFLLAILALLSINVFAQNPIDPLKDLDFSKTSLKQTQ
                     IQPLEMYDLKLLRGIIFGRHGRVFKDSEIKSYLDEQPWYKANPDFKNSMLNDMERRNL
                     DLIRIAEASKHEFIQPGDMRYWRDRQITPRKLGKHSGAEWKVLVAEVEAIHGKRFDDD
                     SWLQQYFEERYWYFPSDKYDAKKLSTIEQKNLQILSDAQKRQRNVALLPGDMEFFENR
                     TITDQNLRGLSLHELRLLRNEVYARHGRMFKAEWLQQYFFQQPWYAPDENFKDEELSG
                     NDKLNVETIVKFENKIHEGLSLAPITRALLEGLFIEDASQMRQEIYARHGKVFKEPWL
                     QKYFSSFDWYKADPNFTEASLTDVEKKNIATIAAYEKRAVTAMSTIEG"
     gene            complement(24833..25372)
                     /gene="rsmD"
                     /locus_tag="VFH96_07785"
     CDS             complement(24833..25372)
                     /gene="rsmD"
                     /locus_tag="VFH96_07785"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET6973910.1"
                     /translation="MRIIAGLYKGRNLKSPPSMNVRPTSDRLRETLFNVIAPRIEDAR
                     FLDLCAGSGAVGIEALSRGASHVTFVDRSRRMCKLIEANVELCRIPEEQWDIYCSEVN
                     EFLRQSKNERWDIVYFDPPYKDDYLRTLGLLGNNDLVIAEHHHKTELPDSIGELQRTR
                     ILKQGDSALSFYSNADERR"
     gene            complement(25369..27633)
                     /gene="recG"
                     /locus_tag="VFH96_07790"
     CDS             complement(25369..27633)
                     /gene="recG"
                     /locus_tag="VFH96_07790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET6973911.1"
                     /translation="MPGAISLNTPIEDLHSFKIARLGPILSQKLAKALATQTHKKNFA
                     EATVEDLLNYFPMRYEDRSRPALIKDLHDGVEASLDLTVTKTNGYAVRNPRGYGRSQL
                     YIFEVYGIDGPMTGREVIVWWFVSGRRAYDIVKYYTAKLTRGTRFITFGRWEWEGRRT
                     TFKLRLNSAADELEILSPPSETPGDDPEQSEENQPDPSLAAIHVGRRVPVYRKLGDFN
                     SKRVREIVHAVLSLLNNKAIEETLPADLRHRAKLIGRAEAVREIHFPPPDVSMADYEQ
                     SKSQAHLRMIFEDFFWVTFAIGLKRGQREKEVKAAPLHIDKTVKAHIASVMPFKLTSA
                     QVKVTKQIFNDMQSNAPMNRLLQGDVGSGKTIVAVVAMMAAMENNLQTALMAPTEILA
                     EQHARNIKRLLAKTPYRVELLTGSLRAAEKRHLQNALKEGAIQACIGTQALIQEAVAF
                     EKLGLVVIDEQHRFGVMQRAELRARGLNPDVLVMTATPIPRSLAMTVYGDLDVSIIDE
                     LPPGRTPIKTLVFGEDQRQEVKRLITREVKAGRQVYVVYPLVEESEKMDLKDATRRYE
                     YLRDTVFPKLTVGLLHGKMKPAEKEKVMSAFVAGEIKILVSTTVIEVGVDVPNASVMI
                     VEHAERFGLSQLHQLRGRVGRGAEKSYCILLTSDKKTAVAEERLGIMAQTNDGFRIAE
                     KDLELRGPGELLGTRQSGLPEFRIGNIVRDQVILEQAKREAEFYLAKPKAVVTAKMVA
                     RIKTDPRFGLARIG"
     gene            27673..28083
                     /locus_tag="VFH96_07795"
     CDS             27673..28083
                     /locus_tag="VFH96_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973912.1"
                     /translation="MDNERASDETRDNLKVLRLGLLRLHKALLDLERRDFERARGRIG
                     NSYEFLQLVLKDPWFDWLHRLSELIVQIDETLDPHSETPATEDDAKALIERARTLLAP
                     SESGSEFQKNYFLALQQSPDVVLLHSEVIKLLGR"
     gene            complement(28143..28541)
                     /locus_tag="VFH96_07800"
     CDS             complement(28143..28541)
                     /locus_tag="VFH96_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HET6973913.1"
                     /translation="MIRPMREDLTRLGAEELKTVEAVDDTIQNSKGTLMVVVNSICGC
                     AAGKARPGVALALQNEVKPDKVATVFAGADIEATERARSYFTGYGPSSPSIALLKDGK
                     LVYMLERYQIEGRDAQQIAGELTRAFEEYC"
     gene            complement(28598..30253)
                     /locus_tag="VFH96_07805"
     CDS             complement(28598..30253)
                     /locus_tag="VFH96_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET6973914.1"
                     /translation="MRPVLSAEQIREIDRLTVENYQTPSVLLMEAASSACLEAIRTRL
                     DNELANRKALILCGKGNNGGDGAALARAMSRAGMHCSVVLFGKLSETSGDARANFDAV
                     ARLASFEAGSPEAPPPMTFIECESVAAWEQIAKPRRAYDIIVDALFGTGLTRPLEGVY
                     LKVIEHLTMLREARERASGVCPLFFSIDIPSGLNADQAKPIGPAVQADLTVTFTAAKT
                     ANVLPPACEFGGELIVADIGSPSSLIAAAKPFLFVTEAEDARAWLASTRYTPDSYKNT
                     HGHVLIAAGSRGYSGAAALCGNAAMRSGAGLVTIATPASAQVSVASSAMPEVITTALA
                     ETDRGVVSDEATNHFLKLAEKATVVAIGPGLTSDDERTRRFVRSVVEQRTTPCVIDAD
                     GLNSLAPWPATLKGSDEHPLILTPHPGEMLRLIDKSDLDDRVATAREFATAHHVVLVL
                     KGARPLIATPGGRVFINPTGNAGLGTAGAGDTLTGLISGFLAQAYGTLKDRANAIRAT
                     VAALYVGGLAGDFAAKRLGMRTMLASDIREHLSEAIRSLDPDV"
     gene            complement(30494..30697)
                     /locus_tag="VFH96_07810"
     CDS             complement(30494..30697)
                     /locus_tag="VFH96_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973915.1"
                     /translation="MYSAPIILPLDWTGRIYVLEDEKGQVVGTGSREVCETLMYMMRA
                     ALSRASENTQVNARSNVRAAITI"
BASE COUNT         6574 a         8619 c         8730 g         6963 t
ORIGIN      
        1 gcacgcacgc tttcgctttc aggcatcgtc ggcgcacaag ggtcggattc gatctggtac
       61 gtcctttatc aacccgcggg cttcctgctg tttttgatca gcggtatcgc ggagaacaat
      121 cgcgcgccgt tcgatctacc ggaagcggaa tctgagctcg tcgctggatt tcatactgaa
      181 tacagtggga tgcgctggtc gctgttcttc atggctgagt acgcggcgat ggtagtcgtc
      241 gcggcagtcg ctaccaccgt ttatctcggc ggctggtatt tcccgtttgt ttatcgactc
      301 gaaacggccg gacatcacaa cctgtttgtg cttgtcagcg tcctcgtctt cctggtgaag
      361 gcttcattga ttctttactt ctacttctgg ctgcgctgga cgttgccgcg ttttcgttac
      421 gatcaactga tggacatcgg ttggaagtgg ctcattccgt cggcgctgat caatatcgtc
      481 atctcggcgt ttgcaatctt tctcgtgcag gctcttaatg gttttcgagg aatgcagacg
      541 atccagtctc tcgatcgcgg gttgaacctg acgggcagtg gtaaagcaat catgatcgtg
      601 atggggctcg tggggctgtt tgcgaccgcc gcactgctct cacgcatcaa ctggcgctcg
      661 cgagatttca atctcaaggt tcaacgcaga gacatcaagc tggtgaacat gccgaagggc
      721 aaacccgcgg tgcagccggg gaattaaata tgccattagt aaaaactccg aagccgacgc
      781 tcggtcagaa gctcaaacgc ttcttcatga tcgatctgat caaagggctc gggctgacgt
      841 tgaaatacaa cctcggcgcg ttcactgaca aagatgcagt ggcgggcaag ggtatctaca
      901 ccgaacagta tccgaaggtt cgacctgatg tggcgccgcg ttttcacggc gcgccgcgtc
      961 tcaacatgga tccggaaacg cacgacacgc tttgcatcgc gtgcaacttg tgcgcgattg
     1021 cgtgtccgga agattgcatc gacgtgattc cgatggacgt cgagatcgtc gtcgccggca
     1081 aaccgcgccg caagaaggtg ctcgatgagt ttatcttcga cacttcacga tgcatgttct
     1141 gcgcgttgtg ccaggaagct tgtccgacaa gttgtttaga gctgacgcag gacttcgagc
     1201 tcgccaccta ctcgcgcgcc ggattcgtct ggaatcgcga gatgctcgag cagggccgcg
     1261 agaccgtgaa gtacacaagc tagttttggc cacaaaaagg cacaaaaagc acaaaatatt
     1321 ttttgccttg agtggcttaa ttctttcaag tggttcggtt ttccattttg tgctttttgt
     1381 gcctttttgt ggctaaaaat gactcttagt ggctacgaag cgcttttttt ctggatcttc
     1441 ggcttggcgg cgatctttgc gggattgctg accgtgatgg cgcgcaatgc tgttcatagc
     1501 gcgctgtttc tgatctcgtc gcttgtttcg gtcgcggcgt tatttattct ggcgcgcgct
     1561 gagttcatcg cgggtgtgca gatactcgtc tacgtcggcg gcgtgatggt cctgtttctg
     1621 tttgtgatca tgctcgtcaa cgtcggcgcc gaggaacgtg gcaccgagga gatcttcaac
     1681 aatcagaaac aagtcacggc gagtttgatc ttctgcctgc tgctcgtcgg ggggatgctt
     1741 tacgcaatca acacgggtta tcgcggtttg caggtgcgct atccggcgac gccggacgca
     1801 gtcgcggcac aggaatcaat ccgagatgag gcacatgcgc cttcagccac aggcagtagc
     1861 cgcatcacga aagatacgga gagcgtaggc agcagtctct atcggtacgc gtcgttgccg
     1921 ttcgagatcg cgagcatctt gttgctggtg gcgattatcg gatccgtgat gctggcgcga
     1981 acactcaaac aggaagcttc ggcggatgat gtcgatccag attttgaatc aaggtaacgc
     2041 ggattaacgc ggatcttaag acgcggattt tatgctcagt ttcttcaaca tcttattgtt
     2101 actgcaggaa accgaaacgc gggttgatct gatgaaacga gcggccgtaa atcccgatct
     2161 gtcgaagttt ctcgtgatcg gcgcgctgtt gttcatcatc ggcgtggcgg gtgtgctaac
     2221 gcggcgaaac atcatcgtga tcttcatgtc gatcgagttg attctcaatg ccgcgaatct
     2281 gaacttcatc gcgttctctc gttatctgtc ggcgatgggg aatgtcaacg cggtcgccgg
     2341 acagatattc acggtgtttg tgatcgtggt cgccgcagcc gaggctgcca ttgggttggg
     2401 aatcgttatc gcgctttacc ggaacaagga aacgatctgg gtagatgaga ttgatttgtt
     2461 gaagtggtaa ttagaatagg acctatagga cgtataggac atatcttaat ttagctctca
     2521 aatgtttaaa tacatctggc tagttcctct gctaccttta atcggcgccg caattaatgg
     2581 tcttctgggc cggaagcttc gcttctctga acagatcatc ggaggcattg ccgtcggttc
     2641 cgtagcgctc gctttcttga ttagcgttgc cgccgtctat tcatatggtt tcggtggtca
     2701 cgcggaatgg cccaagccgt atctcaccag ccaggatggt ttcagcttcg actggattcc
     2761 cggcggtgct gtgagcgtca atcatggcgc ccaatatgca ctgctcaaca ttgagtggag
     2821 ctaccaactc gatccgctct catcgatctt catgctgatc gtcacgggcg tcggcctgtg
     2881 catcttcatc ttcgctaccg gctacatgca cggcgaaacg ggtttctatc gtttcttcgc
     2941 ttacatgggc ctctttatgt tcgcgatgct cgtgctcgtg atgggctcaa acttcctgat
     3001 gatgtttgtc ggttgggaag gcgtgggtct ctgctcttat ttactgatcg gctactactt
     3061 cgatcgacag gaagccgcca acgcttcacg caaggcattc atcacgaatc gcgtcggcga
     3121 cttcggtttc gcactcgcga tcttcggcat catcgcgacc ttcgggagcg cccagtacgt
     3181 caacgtcttc gaccaggcga aaagttatcc gatcgagtta atcggtcact ggggcgtgct
     3241 ctcgtggatc gcgctcggac ttttcatcgg cgcgtgtgga aagtctgcgc aactgccgct
     3301 gcacgtgtgg ctgcctgacg cgatggctgg tccaacgcca gtctccgccc tgatccacgc
     3361 cgccacgatg gttaccgccg gcctctacat ggtcacgcgc accaacgtca tcttccagca
     3421 ctcgcaaaca atgctgctcg tggtcgcact cgtcggcgcc gcgactgcga tcttcgccgc
     3481 cacgatcggc atcacgcaaa acgacatcaa gaaggtgctc gcctattcaa ccgtctcaca
     3541 gctcggcttc atgttcatgg cctgtggcgt gggcgcgttt acgatcggca tcttccacgt
     3601 gatgacacac gcgttcttca aagcgctcat gttcctcggc gcgggcagcg tcattcacgg
     3661 catgcaccac gagcaggaca tgcgtcgcat gggcggcctg aagaagtaca tgccttacac
     3721 gtactggacc ttcatggccg gctggctcgc gatctgcggc gtcattccgt tcagcggttt
     3781 ctggtccaaa gacgaaatcc tctggaacgc tgctaacacg acgcacattc cgctcggttg
     3841 gatcgtctgg ctggtcggca cgatcgccgc cacgtgcacc gcgttttaca tgacgcgact
     3901 gatggcgatg acgttctggg gccgcgagcg atttctcgaa gcgcccgccg gtggtgaagc
     3961 cgacgaagca cacgcttctg cttacgacaa aggtttgact caacacgatg cgactttcgc
     4021 ctcggccgga gatcgtccgc gacacgagcc cggcgagcac gttggaccag tacacgaaga
     4081 cgtggcgcat cacatcgcgt ctcatggtca tggcgggcat cacggcgcgc acataccgca
     4141 cgagtcgccc gcgtcgatgt gggttccgct cgcagtgctg gcaatactcg cgacgctcgg
     4201 tgggttggtc ggtattggac cagcatttcg cacgttgacg ggaaccgagc acccgggcgg
     4261 tcatttgaat atcgtcacct ggctgaatcc gatcatctgg aacccggtca cacgcgagtt
     4321 caatacgcct gagggtggtc acgaagccgc ggcgcccgct gagacagcgc atgccgagac
     4381 agccgcgcca ccagtcgctg aaggagctgc tgaagccgaa cacggcagcg cggctgctgt
     4441 ttacggtgac acgggattca acctggccca cgcagtcgaa ggctcgactc acagcgagct
     4501 cgccacggag tggatcttta tccttatctc gctcgttgtc gccggtatcg gcatgttact
     4561 cggcggattg ttctacgttt ggcgtccggc gctcgcaaat gtttggtcac aacgactgcg
     4621 accactctac gttgcgagct acaacaaata ctgggtcgac gagttttacg gcttgaccgt
     4681 cacgcgtcgc acgatggacc ttgcacgtgc agtctttcgc ttcgattcga gaattgttga
     4741 cggcatcgtc aatggtctcg cctcgctcag ccggttgtgc agtcgaatcg tcggcggctt
     4801 cgacaagtac ttcgtcgacg ggctcgtcaa caccgtcgcc gctttcatca tgcgtttgat
     4861 gtcgccgttg ttccgcgctg cgcagacggg tttcacgcag aactacgcgc tcgtgatggt
     4921 gttgggattg atggtggcgg tggcagtgtt ttttgcgcgc gatattcttg cagtctttgg
     4981 aaggtaagcc gcggatttac gcggattaac gcggatctaa acaatgacta attcgtatat
     5041 tctctctctt atcacctggc tgccggctgt gggcgcggtc ctcattctcg cgctcatgaa
     5101 gaaggaccag tcgagcttga tcaagcggtt tgcgacctta tggtttgcac tcgacttcat
     5161 catctcgctg ttcctgttga aatataaccg cggcggcggc ggcatgcagt tccttgaaga
     5221 tcaccagtgg atcccggtca tcggcgcgcg ttaccagatg ggcgttgacg gcgtcgccgt
     5281 gctgctgatc gtgctgacca cgctgctcgg cgcgatcgcc gcgctctctt cgtggaacta
     5341 cattcaaaaa cgagagaaag agtattacgt gctgttgctg ctgttgcaga cggccgtgat
     5401 tggcgtgttt gcttcgatgg acctgttcct gttttacctg ttcttcgaag tgtcgctggt
     5461 gccgatgtac ttcctgatcg gaatctgggg tggtgagaat cgtctctacg ccgcgatcaa
     5521 gttcttcctt tacacactcg tcggatccgt ggtcatgctg ctcggcgtct tgaagatgta
     5581 cttcctgacg caggacacgg cgatgacggg caagatcacg caggcgacga tcaacctact
     5641 tgccggtaac aacgaggcgg cgcaattagt ttcgagcacg ctgcaagcgg ccgtggtaaa
     5701 cggtacgggc acattcaata tcgtgtacat gcaggcgatg ggatcagtgc tgccaaccgg
     5761 cacgctgcaa gtgcttctct tcgctgcgtt cgcgctcggc ttcgcgatca aagtgccgat
     5821 gtttccgttt cacacgtggc ttccggacgc tcacgtcgaa gcgcccacag ccggctcggt
     5881 gattcttgcc ggcatcctgc tcaaactcgg aacctacggt ttctatcgct tcaacctgcc
     5941 gatgtttccg tgggcctcga aggaccagag ctacttcggc agttttggtg tgcggagtgt
     6001 cgtcgtcttc cttgcgatcg tgagcatcat ctacggcgcc ctcgcggcaa tgtatttcgt
     6061 cgtcaagaaa gacggcgatg taaagaaact cgtcgcctat tcatccgtgt cgtcgatggg
     6121 catggtgatg ctggggctgt ttgcgctcaa tccaaacggc gtgaatggcg gcgtgctgca
     6181 gatgatcaat cacggcatct actccggcgc gctcttcctg ctcgtgggaa taatttacga
     6241 acgtcgacac acgcgtaacg tggctgagtt tggcgggctt tcgcacgtga tgccggggta
     6301 cgccgcgatc tttctcggca tggtaatgac ggcgatcggt ctgccgctgc tggctgtgtt
     6361 catctcggag tttcttgcac tgcgaggagc gttcgaagcg aatccttact gggcggcgtg
     6421 ggcggcgctc ggaatcattc tcaacgcggg ctacttcctt tggctgtacc agcgcatgtt
     6481 cttcggcacg atcgacaatc cgaagaacga gaagttgact gatctcagcg ggcgcgagtg
     6541 ggcttacatg gtgccgttga tgattctcgc tttgtggatc ggcgtctatc ccaagccgtt
     6601 tcttgagtac attcaaaaac cggtgaatgc gctggtgcag caagtcagac ccgattacgt
     6661 gatacctgga caggcggcgc cggcccgaac cgagcgcgca gaaaggtgac gagttaatcc
     6721 acagatttca cagatttctt tctgtgtaat ctgcgcaatc tgtggatagt ttcttatgtt
     6781 tctactacaa gccaacacca cctcgctcat taatgtcagc gatctgcgat tgattgcacc
     6841 cgagttgatc ctgactgtgt ctgcatgcgt ggcgctggtg atggaagtca ttctgccgta
     6901 tcgcaagagc aagctcaccg cttacttcgc gcttgccggc atcgcacttg cttttgtttc
     6961 gctcgcgttg cagatgaccg gcccgatgaa cgtctccggc ttttacggca tggtgcgaat
     7021 cgacggcttc gccatcctgt tccgaataat ctttctgatc tccgcggcac tcgcgatcgg
     7081 tatctctacg cgcttcctcg acatcgaagg cgagcaacac ggcgaatatt actcgctcat
     7141 tctttttgcc accgtcggca tgatgttcat tgcctgcggc tacgatctca tctcgcttta
     7201 catcagtctc gagttgatgg cgctgacgtt ctacgtgctc gttgctttca cgaagcgaga
     7261 gaagaagtcg aacgaagcgg cgatgaagta cttcctgctc ggcgcgtttt cgtcgggtgt
     7321 gctgctttac ggcatgtcgc tgatctacgg gctggccggc tccacgaacg taggcgagat
     7381 cggaagtaat atttcggaga tcgtaaggaa tatccaggca gcgacagccg atcccaccac
     7441 gaatcaacaa atcatccagc tcaggccgtt gttgctgctc ggcatgatcg cactcagcgc
     7501 ggggttattc ttcaagattg ccgcggtgcc gtttcatatg tgggcacctg acgtttacga
     7561 aggcgcgccg acgtcagtca ctgcatttct ctcgaccgga tcgaaagcgg cgagcttcgc
     7621 gttgtacgcc cgcatcttca gtgaagcact gccggccatg cgcgcggatt gggcgccgct
     7681 gctcggtctc gtcgccgcga tcacgatcat ggtcggcaac tgggccgccg tcacccagga
     7741 aaactcaaag cgtctgctcg cttattcttc gatctccaac gcgggatatc tgttgctggc
     7801 gctgatcgcc aacaacgagt atggcaggat cgggttgatc gtttatttat tcgtctacac
     7861 gctcatgaac atgggcgcgt ttggcgtgat tatcagtttg cgccggcgcg gcatcatcgg
     7921 cgacaacgtc gacgacttaa ccggcctggc ccacaaagcg ccggtgatgg ctgcgatgat
     7981 ggcgatcttt atgctgtcac tcggcggctt gcccgtgacg ggcggtttta tcggcaagta
     8041 ttttcttttc ggcggtttga ttcagcaggg cgcgatcgag cataagtcgt ggtattactg
     8101 gctggctggt tgggccatca tcaataccgt agtttcgttt gcttattact tccgcttcat
     8161 caaggtcatg tatcttggcg atcgcatcgc cgacaacaaa ccgctcgcac tttcaccggc
     8221 gctgcaagtg gcgttagcga tcgcagtgat cggaatcata atcattggcg tttatccgca
     8281 gccgttcatt gcgctcgcaa aaacactcat gccctcgtag tgatgcatcc gtgtgatggt
     8341 cgtctactac ttcctcgcag tcgtatcctg ctggttcggt ctgcaatcgt tgttgagcgg
     8401 catacgttac gcggcttacg tgcggcgcga gacgagccgt ccgcttcctg atttccaacc
     8461 attcgtttcc gtgatcgcgc cgggcagggg actggagccc gggctgatcg acaacctgcg
     8521 cccgctcgtc gcgcaggatt atgcgcgtta tgaagtgctg tttgttttcg acgcgctcga
     8581 cgatcccgcg atcaagatcg ttgacgagct gaaaaatgca gctacacgca caatcatcgc
     8641 cggtccggca accgacagcg gccagaaggt tcacaacctg cgcgtcgctg taaacgaggt
     8701 cgatccgcaa tcacaagtgc tggtctttgt tgatactgac gcgcggcccg caaacgactg
     8761 gttgcggaaa ctggtcgcgc cgctcgctga cgagtcgctt ggcgcctcga ctggttatcg
     8821 ctggttcatt cccgatcgcg gtggtatcgc ttctcgtctg cgcagtgttt ggaacgcgtc
     8881 cgtcgcatcg gcgctcggtg gtaacactgc taaaaacttt tgctggggcg gctcgaccgc
     8941 aattcgtcgc acgactttca ccaaactcga cgtgaccgag cgctggcgcg gcagcgtctc
     9001 cgacgacttc acgatcacgc acgttctcaa acaagcaaac ctcccgatcc acttcacacc
     9061 taactgtctc gttgcctccg tcggcgactg cgactttcga gagctgatcg aattcaccac
     9121 ccgccagata aaaatcacgc gcgtctacgc ctcccatctc tggctccctc ttctccttgg
     9181 ctcctccctg ttcgccatcg ccttcttcgg cggcctgatt ctgatcggcg ttcatccgcg
     9241 ttcatccgcg gcttatattt ttccgttaat catcttcgcg ctcggcgcag ccaaatcctt
     9301 catccgcttt cgcgccgtct ccaccgttct caaaacatca cgcctcgatc tcgtcgccca
     9361 catccttctc tggcccatcg cgtcgttgct ctatctttac aacgcggtgg tcgcgggctt
     9421 ttcccgccgc atcaagtggc ggggtatcac ctatgaattg aaatcaccaa ccgaagctgt
     9481 cattatctcc cgcgagtctt aatgcgcaac aaagccgcac aattcgtgca actgcttcga
     9541 tcgacgaagc gcgaaaagcg tctcggtacg gtgatcttat tcgtcacttc gcgctgcaac
     9601 gccttctgcc agacgtgctt ctatcacgaa gagttgaacc agccgggcga catgacgttc
     9661 gcacagatcg agaaaatctc gcggaccatg ccgccgatca cagacctttg gctctccggc
     9721 ggcgagccga cgctgcgacg cgatctttcc gacgtcatcg atctcttcgt caagaacaac
     9781 ggcgtcgatc gcatcatcat tcccactaac ggaataatca aaacgcgtgt gtacgacgtt
     9841 gtcgatcgcg ctctcggaaa caacccgcaa atcgatctct atctgaacat cgcgctcgac
     9901 ggttacggcc agacgcacga tcgcatcagg ggcgttcccg gtaactggga aaaagcgctc
     9961 gcctgtatcg agtcgctcta tgcgctgaag gagatgtaca ccgatcgttt tcgtttgaac
    10021 gtgaacacgg ttgtctgcgc tgaaaactac accgagatcg agctgctctc cgagttcatg
    10081 tggtccaact atcgtttgga tgggcagtat ttcaacatca ttcgcggtga aacactcgtc
    10141 ggcgaaacga tcaaacaagt gccgccggaa gttctgccgg cgatgtacgc gcacgtttcg
    10201 aagctaacaa agcgctacgg cgagcgaatg tttgccgctg acgacgcgag caagcgcttc
    10261 gtgaagaacg ccgtgtacgc cggcacgatc atcactcact accgcaccca acacgcaaac
    10321 ttccagcagc cgactgcgtg gacgtttccg tgtacggcgg ggcaaacgac agcagtgatc
    10381 gactataacg gcgatgtgcg tgcgtgcgag ctccgcgaaa agtttgcctc actcgctgat
    10441 ttcgatttcg acttcaatgc tctctggagt gcaaacgacc gccaaacaga actcaccgcg
    10501 atcgacggcg gcaaggcgtg ctggtgcacg cacgtgtgct tcattcacga ctcgatgcgc
    10561 cattctcgtc gcgccatgct ctacaacgtt cccaaaaact acctgacacg cgaaagctgg
    10621 tgatcaaaaa agaagaccag gacgagaacc gaaaagcggg cttcgcctac gcggctggca
    10681 tcgctctctt cgcctccgtg gtcacgttca gcgccgccgg ctggttcgtc gacaaatggt
    10741 tcggcatcgc cccctggggc ataatcggcg gcatcgtcct cggctccgcc gccggcctct
    10801 tcgaattcgt gcgtatcagc tcaaaaactt attagaatcc gcgtattaag atccgtataa
    10861 tccgcggctt aactcgacaa ccaccctaaa tcccggctaa aatcagcggc tttgcaatgc
    10921 tggagtcccg agtctttcgc acgatggcga ttgcgagcgc gctgagcgtg agtgtcgcgg
    10981 ccttcatcgc gccctggcgt gtgacgacgg gcctgttgtt gggtgggttg ctggcgcttt
    11041 ttagtcatcg ctggctctca aactcagcct cggctgccgt tcagctttcc gccaccggtg
    11101 gccggtcgca atggaagctc gcgcagtttg tgcttcgtta cgtcgtgatc gccgcagccg
    11161 tttttgcagc ctacacacta gatctcgtgt cgctgacagc ggtcctcgcc ggcatgtgca
    11221 gttttgttgt agcaatgttt gttgaagcaa tcagagagtt ttacttcgca atcataaagc
    11281 gagaggaaaa ttagttgatg ttttggtttg cagaagctgc ggaagccgcg cacggagccg
    11341 ccgagcatgc agaagaggcg gagcatcata cgcccatcat cgttgagttg gttaatcact
    11401 acttcggcga gtatgcgtat cgttttgaga ttgcgtacac gaagccgttt tgggacaagt
    11461 ttttaggctt cttcggcacg acgcctgaag atgccttcgg accgtataca ccggagaacg
    11521 caattccctg gtacacggtg atgttcgtca tcgcctgcat cttgagcgtc gtcattatct
    11581 ggattctcaa gggcaagctt tcggaaagta aaccgggcca cggccagcag acactcgaag
    11641 tcagcgtcct ggcagtgcgc agcatgatcg aagacatcat cggcccacac ggtttgaaat
    11701 acttcccggt ggtgatgacg ttcgccgtac tgatcctcgt ctcaaacctg atgggactgt
    11761 ttccgctgtt catgagcccg acatcagcga cgagcgtcac tttcgcgctc ggtttgagct
    11821 cattccttta ctacaactac atcggcatag ccgagaatgg tttggtcggt catctgaagc
    11881 acctcgctgg tcccatctgg tggatcgcgc cgctgatctt tccgatcgag ctgatcagta
    11941 acttgattcg accgttttca ctcggcatcc gtcttttcgg taacttgttc gcggacgaaa
    12001 aggtgctcga aacattggcg ggacttcatc cgtggcagtc atggtttgcg ccgattctgt
    12061 tgatgccgct gagcgtgttc gtggcgttga ttcaaacatt tgttttcata ctcctctcgc
    12121 agctttacat cagcgaggtg tcgcatgcgc cgcatcatca cgagcatggc gcagacgacg
    12181 aagaagacga gtatgacggc gacgaatata tagctccagt tcttacttaa tatatggctt
    12241 tatcaaagcg tgcggtgagc gatgcgggca gcgcgccccc aaacgttacg cacattacga
    12301 gattcatccc gaggcatacc agacactcat agcaagtcat gtctgcttcg caggagcgcc
    12361 gttgtaagag gggcggtgga taccggatgc aggcgaaata atatcgtcgc ggtttgctcg
    12421 gacaggtgag ttgaaacaaa tcagaggagg aagcatgagc aaatttagaa tgttgctatt
    12481 tgcggtgatc ggaattctcg caagcagcat gagtgtattt gcgcaggcgg gtgcggaagc
    12541 cggcgacaac gtgtattccg tcaacaagta cagagcaatc gccgccggta tcggtttcgc
    12601 gatcgccgtt tttggcggcg cgctcggaca gtcgcgcatc ggtgctgcgg cttgtgaagg
    12661 cgcggcgcgt aatccaggtg cagcgggtcg tatccagacg atgatgattc ttggtctggc
    12721 gctgatcgaa tcgctggtgt tgtttgcgct gctggtgatc ttcgcggtcg tcaaaggcgt
    12781 tgtcacgccg tagttgagtc gcgatcagac ttcgcatgag cgggcggggg attcgtctct
    12841 cgcccgtgtt tttttagaga agtacatgat ttgcaagatt ccgatgattc ggagtttaat
    12901 gaactaaatg aagacatgtc cgaaatgtgg agttgaatat cctgacacga caacgctgtg
    12961 ccccgccgac ggcgttgcgc tcgaaaaaga tcccgactcc ctcgtcggca ctacgctcgc
    13021 cggaaaatat cgcatcgacg cacggctcaa cgaaggtggc atgggcactg tctatcgcgg
    13081 ctgtcacgtg ttgatggaca agacagttgc gatcaaagtg ctgcgcccgt cactcgctgc
    13141 tgatgaaaag atcgtggccc ggttctcgcg cgaggcccga gccgcatcgc gtatcacgca
    13201 tccgaatgcg atcagtgtta ccgattttgg tgaagacgaa cgcgggcacg tcttcctcgt
    13261 gatggagtat ctgagcggta agactctcaa gcacgtcatc cgcgaggaag gcccgttgcc
    13321 gctcgcgcgc gtagttgaga tcacgcggca gatcggcgac gcgctaaacg ccgcgcacca
    13381 acagggcatt gtccatcgcg atctcaaaag cgacaacatc atgttggtcg atacgatgac
    13441 gggcgatcac gcgaaggtgc tcgacttcgg tatcgcaaag atcaatgaac ctgacggcgt
    13501 cgtcgataca aacctgacgg ctccgaatct cgtcatcggc acgccgcaat acatgtcgcc
    13561 cgaacagtgc tcgcaggatg tcgagattga tgcgcgctcc gacatctact cgctcggcgt
    13621 gatcctgttc gagatgctcg tgggccatgt gcctttcagc ggcgactcgc cgacgatcgt
    13681 catgatgaag cacttgcagg acccggtgcc atcggtactc gaggaacgaa gcgatctgcc
    13741 tgcgtctgtg gcgcgagtgg tcgcacgagc gatggcgaaa gttcgtgaca atcgttatca
    13801 aaacgtggct gagttgattg aggacctgac gatcgcatcc ggcatgacga tacacaggct
    13861 gggtccggtt ggtgtgacca gcacggtccc gacgcccgat cccgatgaag tgacggtggt
    13921 cagagcgagg gaagtgatgc cgccggtttc gccagtgccg ccggttcagc gacgcgagcc
    13981 ggtggtggtg acagtttccg gccagatgcc gccgcccgcg cctgctgctg cgggtttcaa
    14041 tcctttgaag gtgctgattc cgtcggtgat cgggttgctg gtggtgtttg cggcgatata
    14101 cgcgttgacg aggaatacga cgccgggcga ggccaataca aaccagcaag tggtgccgtc
    14161 gctcgcggcg gatcccaaca gccagccggt gcagcctgcg caaccggcga cgggcaaagg
    14221 tgaagaggga ataccgtcgg gtggatcgat tacaccgccg gcgaatgcga gtccgagtgc
    14281 gagtgtgtcg gcttcaccgg caccgactga ggacttcact ccggacgcga acgcgaacag
    14341 taacacgaat acaaactcta atcagaacag caacggcaac agcaatcgca aagcacccgc
    14401 attgcctgag cctacgcgca gcgtcgtccc cgacaaccca ccaccgcccg tgccatcagc
    14461 gacaaagcca ccagcagaga agccctcgcc aaccgcgact cccatgaagt agttatccac
    14521 agattacaca gatgtctcaa acactctccg tagtctgcgg ataaacccgt ctgcatcatc
    14581 gatcgcgatc tcgatctcca ggacgtaaca cggtcgcgag aactccatcc ctttcaactt
    14641 aaccgcatcc ttcgcggtgg ttatcaacgc actcgctccc gtctgcctcg caagctgatc
    14701 caacgcatca acatcactct gcgaataagc atggtgatcg gcaaaggctc tttccactcc
    14761 gagctcataa cccaaacgct tcaaactctc aaaaaacgac gacggattac ccaccgcaca
    14821 gaacgcacca acccgccccg gcaaggcaat cgtctccggc ccgtttttca acgacgtcac
    14881 acgcagcggc cgcatccgcg attcgaagat cggacgcccg ccagtcagtt caacgatctc
    14941 cgctcgcaac gcttcgacac tttcaacctg atcgcacctc gtcagcacaa aacaatcagc
    15001 ccgagttaga ccatcaagcg actcgcgcaa gcgtccgtaa ggaagcaatc ggccgccgcc
    15061 ccacggatta gtcgcatcga tacacacgat attcagatcg cgtgccagtc gcaggtgctg
    15121 aaacccatcg tccaacacga aaacctcagt gccgaagtct ttgatcgctt cctgtccggc
    15181 cgctatccga tcggcgctgc tgatcactgc tgcctggccc gtgagtcttg tcgcgagcag
    15241 gtacggttca tcgcctgctt cgctgggcga tgcgagcacg ccataaccgt ccgagacgat
    15301 cacctgaaga tgcggatctt ttcgaccgta accgcgcgtc agtatgcaca cctttttacc
    15361 ttgcgacgcg atcgttctgg cgacgtactc gacgagcgga gtctttcccg tgccgccggt
    15421 ggtgatgttg ccgatgctga tcaccggccg atcgagtttc gtagtctgaa aagtcccacg
    15481 acgatacaac gacagacgcg tgcgcgtgac ggcgccgtac agcaaactca atgggggcag
    15541 gacgattgac ttcatgaagt ggcggcaaga ataggcggtg cgatcaattg catcgtgcga
    15601 tcagcggcgc cctggttagc ggtgatcagt tcttttgcgc gtcgacccag ctcgtctctc
    15661 tctgcggggc tggcgagcag tttctcgaaa acgtaggcaa gctcgtcgct tgcggctgcg
    15721 tcttcgagcg cgggcagttg cacgagcgcg cccgcttcgt tcatcagctc gacgatcgcg
    15781 tggaagttat gcgtgtgtgc tccggtcaca acggtcgcgc ctgcggctgc gggttcgagc
    15841 acgttgtgcc cacctttatc gacgatgctg ccaccgacga agacgatctg ggcgagcgca
    15901 taagtcgcgg ggagctcgcc gatcgtatcg agcaggatca cgctggcatc ggcgtcgttc
    15961 gcgttgggcg gattcgttcg tcttgtccag ctcagtccgg atttttgcag taatcctgcg
    16021 acttcgttga agcgctcagg atgtcgcggc gcgagcatca gtcgcgctgg ttgtttctgt
    16081 ctgagcttct gaatgctttc gagaattatc tgttcttccg gcgcgtgtgt gctggcagcg
    16141 agaatgagcg ggcgatttga atcgaaggcg aatcgatgcc tgatctcttc ggttttactg
    16201 gtcaactcgc tcgacagatc cgcgtcaaac ttgagattac cagcgttgaa gagtctgtca
    16261 ctcgccatgc cgagcgcttt cagtcgctca agatcagtct cagattgcat tacagcaatg
    16321 ctgagcgacg aaagcacgcg acgaagaaag aacttgatta gcctgtagcg gcggaatgac
    16381 tgccgcgaga tgcgtccatt gacgagtgcc accggaatgc gtctcgcgtt gcactcgcgc
    16441 aggaagttag gccagagctc agtctccata atcaacacga ctgccggatt cacggctttc
    16501 agcgcgcgac gaacggtcca acgccagtca aacggaaagt aaaagacgct ctcagcctgg
    16561 tttcgaaaga catcgcgcgc gaggttttgc ccggtcaacg taatcgtcga gaccacgagc
    16621 gcatgatgcg gaaactgctg cttcagtcgt ttcgcgagcg gtcgtgccgc ctgagtctca
    16681 ccgactgaaa cgcagtgcag ccagataacc ggcttgccgg tgactggagg aaccgacccg
    16741 agacgctggc gcaatccggc gatgtatttg ccgtgcgcca gcgcttgaaa aaggaagtga
    16801 ggaatcagaa cgatcaggcc gagtgatagc agaaggctgt aagccaggta catacgccgc
    16861 tcacttcgct cgctcgatat accgtccttc cgaaggatcc acgcgaatgc ggtcgccctc
    16921 gttgatgaac ggaggcaccg taatggtcac tccgttttcg agagtggccg gcttgttaac
    16981 gtttgagact gtcgcgctct tcaattgcgg ctcggtttga gtgacggtca actccatcga
    17041 cggtggcaga tcgatgccga tgggtgcgcc ttcgtaaaac tccgcctgga ttctcatgcc
    17101 cggcatgagc cacggggctg cgtcaccgag ctcttcttca ctcagtgctg tctgctcata
    17161 gttttcagtg ttcatgaagt ggtggtgcga gccgtctgag tagagatatt ccatctcgtg
    17221 ctgttcgagt gacgcgcgat cgactgtgtc ggcagagcgg aagcgatgtt caaatgaagc
    17281 gccggtacga atatttcgta gcttagtttg gatcatcgcc cggagattcc cgggcgtgtg
    17341 gtgtctaaat tcaagaactt tgcacggttg gccctcaaaa acgataacca taccgcggcg
    17401 aatctgtgta gcaggaatag ccatatactg aagtctccgt ctaaaaaggt agggctttag
    17461 caaaatgcgt agtttaaggg gcgggattgc gggttgtcta gtcgccgaag agataggtcc
    17521 ggtatgtccg acatgcttta gcttgtcggg attgcgcgac aaactgaagt ttgtcggaca
    17581 ctttggaaag aagaagggcg acctcctgaa aaccactcca gagagaccgc cctttttgag
    17641 gagcccacct acctcggtta ttagaacgct attggccgaa tttagttcca ggacttaggc
    17701 tgcgcgggcg cgcgggacgc ggcgcaggcg cgcgaggcgc tcgagccact tttgcgactc
    17761 ctcgtagtac ggaaacttgc gctcgtcctg acgaaatgcg cttgtgtggt aatagcgtcg
    17821 cccattgatc aaacgtgccg gatggatgat gtgcagcatc tcgtggtaca tgatgtactc
    17881 tgcaaaccac tccggaatat ccttcgaatc aagcgtcttg ctgatcgtga tgctgtcgta
    17941 gatgtgatcg tgatgaccga gaatgcttct ggtccggcgc tgcgaccacg taatcgtcgg
    18001 ttttggaatc gcgccgtcaa aatatcgtcg attcaacttg gcaaacattc gatcaagatc
    18061 gtaaacctca ccctgcgcgg tcgagatcat ctttcgcccg cgaccgcgac gcgccagatc
    18121 agacgagcgc atcaccgacg gcgtcagcga ataatctcga taaactcgat cgtgaatcga
    18181 cggcacgcgc ttgccgagca gtcgcgctac caggatccac gcaagcgcgc gcaacacctc
    18241 aggcggcgag tctttacaga tatccgagag ccgcacgtaa acgcgtcccg agcgcaaacg
    18301 aatggtgtga cgcagtcccg cataaggata aaagcggact tcaatcgcgg gcacaccacg
    18361 attcccgccc atatgtcgaa atgcgtcggc gaaatatttt ctaacttcat tcattgcggt
    18421 taactcggag gccaacagcg gttcgggtga ctaatcttaa ggatggcgga cgcggcgcgt
    18481 caacctttgg cgggcgttct caataagcgc ctcattttca acaacttaga tgcctccaca
    18541 gattgcacag attacgcaga aaggggctaa tctgtgcaat ctgtggataa gtttgtatac
    18601 tgccagcaat gccccggaga gcctcaaatc tgcaaactac tgagacggcc ggcttagact
    18661 cccgcgcgga ggtgctactc gccgccctga tcaaagaaca cctcgtcact ggcgaggccg
    18721 tgggctcgcg tgtgctctcc gatcgcttct cgcatggctt tggctggagc ccggcgacga
    18781 ttcgcaacgt gatggctgac ctggaagagt caggtttcgt cgaacagcca cacacttccg
    18841 cgggacgcgt gccgactgac aagggttatc gttactacgt cgacaacatg ctcggcgagg
    18901 cgcgtttgtc gcgcaccgat ttgaaagcga tcgaccatct cttcgagtcg atcgacgcgg
    18961 gttcgtcacc tgatcggctg atggaaacag cgtcgcacgt gctctcggca ctctcgcaga
    19021 acgtcggcat cgtcgtgtcg ccgtcgctcg cagacaaccg gctgaatcac atcgagttta
    19081 tgttgctgcc ggaccatcgc atcctcgtag tgctcgtctc aacggcaaat atcattcata
    19141 acaaggtgat tcgcgtcgcc ggcgatgatc taacccagga cgacctggaa cacaccgcgc
    19201 gctatttgaa tacggagttc agcggcaaga gcctgctcgc gatccggacg gagatcgtcg
    19261 aactgatgaa agaagagaag gcgctttacg atcgactgct gcaaaacgcg attttgcttt
    19321 gtgagcgaag tctcgaaggc gaggacacga gcgccggagt ttatgtcgac ggcacttcaa
    19381 acatgctgac gaaaccggaa ttcgtcgaca tcgagcgtat gcgcgagctg ttccggacgt
    19441 ttgaagagaa gtcgcgcctg atcaagatac tcaacgagtg cgttcacgac gcagcgggct
    19501 acccgggcga cgtacacgtc attatcgggc gcgagcatcc cgccagctcg atgcgcgact
    19561 gcaccttgat cactgcgcct tatcgcgtcg gatcgaacga caacatcggc tcgctcagcg
    19621 ttgttgggcc gatgagaatt gaatatgcac gaataatggc tatggtaaac tacatggcgc
    19681 ggttgattga acatcgactg ggcgaacaaa ctgcacgtta gtccgagaat cgatcctacc
    19741 taatgacatc agacagacgc gacaagggtt cgcaccgcat cccgatcaag ttcaccgacg
    19801 ctgacgacat ggcagccgaa gatccgaatt acgaaggcga ggagattgac ctttccgacg
    19861 agagtctcaa cgaagaagac ctcttttcgg ccggcgacgt cgagccggaa ggtggtggac
    19921 ccgaaaaagc cgagctgatt gcgacgcgcg cggagctgaa gcgaatcgaa gctgaaaacg
    19981 ctgagctcaa ggatctcgtc gcgcggcgcc aggcagattt cgagaattat cgaaagcgcg
    20041 tcgaacggga acgaagcgag acgtacaatc gcgtagttgc cgatgtggca gcaaaactcc
    20101 tgccggtagt ggacaatctc aagcgagcgc tggaggccga agcgtcggtc gaggccgcgg
    20161 agtcagacga gtttcgtcat tttctcagcg gtgtggatct catcaacaag cagctgaatg
    20221 gcgtgctcga cgcgctcggc atcaagccgg ttgcggcggt tggtgaacag ttcaatcccc
    20281 atgttcacga ggctgtggta actgaaccaa ccgatgacta tgcacccgac acagtaatgc
    20341 aggagttagt tacaggttac cgcctcggcg acaaactcat tcgacccgcg ctcgtgaaag
    20401 ttgcaacacg taagtgaagc cacaaaaagt cacaaaaagc acaaaatgcg atccgcttcg
    20461 atgagcctgt ctgatattcc ttgttatgtg ccttttgtgg ctttttgtgg ctaattctta
    20521 atcatgggaa aatcgatcgg cattgattta gggacgacca actgctgtgt cgccgtgctc
    20581 gaaggcggca cggtgcagat tgtcccgaac aaagaaggtg ggcgcacgac tccgaccgtc
    20641 gttggtttca cggaaaaagg cgagcggctc gtgggccaga tcgccaagcg ccaggcagtc
    20701 accaacgctg ccaacaccgt tcatgccgtc aagcgactca tcggccgcaa gttcagctcg
    20761 cgggaagtct caaagatgcg cgagagcgcg cccttcgaga tcgtcgattc tcctaacggc
    20821 gatgcgcgcg tgcgcgtgca gggccgcaac tacagtccgc cagagttgca gggaatcgtt
    20881 ttgcaacgtc tcaaagcagc cgctgaagag tttctgggag aatcggtgac tgacgcgatc
    20941 atcaccgttc ccgcatattt cgacgacacg cagcgacagg cgaccaaaga cgccggcaag
    21001 atcgcgggtc tgaagatcga gcgcatcatc aacgagccga cagccgcggc gctggcgtat
    21061 ggactcggca agtcaagcgt cgaacggatc gcggtttacg atctcggcgg cggcacgttc
    21121 gatatctcga tccttgaaat gaacgacggc gtgttcgagg tgctctcgac ttcgggaaat
    21181 acgtttctcg gtggcgagga tttcgacgag cgcatcatga gctggatggc aggacagttt
    21241 ctctccgaca ctggcatcga cctgcgcgaa gatcgcctcg ccctgcagcg cttgaaagaa
    21301 gtcgccgagc gcgcgaagtg cgatctatcg accacaaacg aaacggtaat caacctgcca
    21361 ttcatcgcag ccgacgcgac cggtccgaag cacttcaaca agacgctgac acgcgaacag
    21421 tttgagacgc tcgtttccga tctcgtcgaa cagacgatcg agccttgcca gacggcgctg
    21481 aaagacgcga agctacaagc gtcagacatt cacaaggtga ttctcgtcgg tggccaaacg
    21541 agatctccca tcgttgagcg cacggttgaa aagatcttcg gtcgcaagcc atcttctgag
    21601 atcaatccgg acgaagtggt cgggatgggt gcggcgatcc agggcggcgt gttgaatggc
    21661 gacgtgaaag acatcgtgct gctcgacgtg ttgccgctga gtttgggact ggcgacgcgt
    21721 ggcggcttgt tcacgaagat catcgaacga aactcgacga ttcctttgcg cgagagccgg
    21781 acgtttacga ctgttgtcga taaccagtcg tcagtcgaga ttcacgtgct gcagggcgag
    21841 cgcgaagtcg cggatggtaa tcgctcgctc gggaaattcg agctcgtcgg gattccgccg
    21901 aacccgcgcg gcgtgccgca gatcgaggtt tcgttcgaag ttgacgctaa tggtatcgtg
    21961 tcagtttccg cgcaggacaa agccaccggg cgtgagcagc agatgcggat cacgccgacg
    22021 tcgggcctga atcccgagga gatcgacagg ctgatccacg aagcggagag tttcgcggaa
    22081 accgacaaga acacgaaaga ggttttggtg ttgcggaccc ggctcgattc gttgctgcga
    22141 aacacgcaga agtcgtttac gaagtttggt ggcttgctgc cggagatcga tcaggagagc
    22201 gcggacaagg cgttcaccga agccgaagcc gcacttaagt cagacaagat ggacgaaatt
    22261 aaaatcgcta tgaacaaggt ggagcgcgtc gccgggcaac tcacatccgc aatgttggac
    22321 cccgaagccg acgcggtcga atcttgatga tcctgtaaat cctgaaatcc tgtaaaagac
    22381 ttagacagga tttcaagatt tacagaatta ataagatatt aagaatatga gcaaaagaga
    22441 ttattacgaa gttttagccg ttagccgcac cgctacggac caggaaatta agagcgctta
    22501 ccggcgtctg gcagttcaat atcacccgga taaaaatccg aacgacgcta ccgccgaaga
    22561 gaagttcaag gaagcagccg aagcctacag cgtcctctcc aatcccgaac agcgcaaacg
    22621 ttacgaccgt tttggtcatg cgggtgtttc gagtggcgcg gctggcgcag gcgcgtgggg
    22681 cgcgcccggt tttggtggca tcgaagacat actcggcgac ctgtttggtt ttggggacgt
    22741 gtttggcggg cgcggtggcc agcgtcgcag ctcagcacaa cgcggcgccg atctgcgtta
    22801 cgacctcgag atcacgctcg aagaagccgc gaatggaatg acggcgcaac tccgcattcc
    22861 gcggctcgag tcgtgcgata cgtgtaaagg ctcgggcgcg gcggcgggaa gccacgcgga
    22921 aaactgtacc acttgtgcgg gcactggcca ggttcgttac cagcagggat tcttctcggt
    22981 cgcgcgaacg tgccacgttt gtcgcgggac gggcaaagtg atcaagaacc catgtccgga
    23041 ttgtagcggc acaggccggg tcgagcgcga gaaacagttg gaagtaaaag ttccggcagg
    23101 agtcgaaacc ggctctcgtt tacgcgttac tggagaaggc gaagctggcg cacagggtgg
    23161 cgcgactggt gatctttatg tggttatcca cgtagccgag catgacatgt ttgagcgaca
    23221 aggtagcaac ctgtacgagg cgatcccgat caccttcgcg caggccgcgt tgggcgccga
    23281 ggtcatggtc aagacgctca tcgacaaaga agagaagttg aagatcccga tgggcactca
    23341 aaccggcacc gtctttcgct tacgtggaaa gggcatgcct gcactcggcg ggcgcggtcg
    23401 cggcgacctg tttgtttcag tgactctcat cacgccgacc acgctgacgc gcgaacagcg
    23461 acgcctgctc gagcagttag cagaagttga aaataaggat ctcgaaagta aaggcctggt
    23521 cgacaaagtc cgcgacatct tcgggtaacg atccgcgttt ttaatccgcg ttcatccgcg
    23581 ttccaaagtg aggaaaccat gttagttcgc ccccgacact ttttcctgct tgcgatactg
    23641 gctttgctgt ccatcaacgt gttcgcccag aacccgattg acccactcaa agacctcgat
    23701 ttcagcaaga cctcgctcaa acagacccag attcaaccgc tggaaatgta tgatctgaag
    23761 ctgctgcggg gcatcatctt cggccgccac gggcgcgtgt tcaaagacag cgagatcaag
    23821 agctatcttg acgagcagcc ctggtacaaa gccaatcccg acttcaagaa ctccatgctc
    23881 aacgacatgg agcgtcgcaa cctcgacttg atccgcatcg ccgaagcttc caaacacgaa
    23941 ttcatccaac ccggcgacat gcgttactgg cgcgatcggc agatcacgcc acgcaaactc
    24001 ggcaagcata gcggcgctga gtggaaagtg ttggttgcgg aagtcgaagc gattcacgga
    24061 aaacgtttcg acgatgactc gtggctacag cagtatttcg aggagcgtta ctggtacttt
    24121 ccgagcgaca agtacgacgc gaaaaaactc tccacaatcg agcagaagaa ccttcaaatc
    24181 ttgagcgacg cgcagaagcg acagcgaaac gtagctctgc tgccgggcga catggagttt
    24241 ttcgagaacc gcaccatcac cgatcagaac ctccgcgggt taagcctgca cgagttgcga
    24301 ctgttgcgaa acgaggtcta tgcgcgtcat ggtcgcatgt tcaaggccga gtggttacag
    24361 cagtatttct tccaacaacc gtggtacgcg cctgacgaga acttcaaaga cgaagagctt
    24421 tcgggcaacg acaagctgaa cgtcgagacg atcgtaaagt tcgagaacaa gattcacgag
    24481 ggcctgagcc tcgcgccgat cacgcgcgcg ctgctcgaag gcttgttcat cgaagacgcg
    24541 agccagatgc gacaggagat ttacgcgcgt cacggcaaag tcttcaaaga gccgtggctc
    24601 cagaaatact tctcgagttt tgactggtac aaggccgatc caaacttcac tgaggcgtca
    24661 ttgaccgacg tagaaaagaa aaacatcgcc acgattgcgg cgtacgaaaa gcgcgccgta
    24721 acggcgatga gtacgataga gggttgataa ggcatctgga tcccgcgtga cgggatcgaa
    24781 acaagaaaaa aagccgcgga tgaacgcaga tgaacgccga tctgatcagc gtttatctgc
    24841 gttcatccgc gttactataa aaactgagag cagaatcgcc ctgtttgagt attcgagtgc
    24901 gttgaagctc accgatcgaa tcaggcaact cggttttgtg atgatgctcg gcgataacta
    24961 aatcattgtt cccgagtagt ccgagcgtcc gcagataatc atctttataa ggcggatcga
    25021 agtaaacgat gtcccagcgc tcgtttttag actgccgtaa aaactcattc acctcggaac
    25081 aataaatatc ccattgctcc tccggaatcc tgcacagctc cacattcgct tcaatcaact
    25141 tacacatccg tcgcgaccga tcgacaaacg tcacatggct tgcgccacgc gaaagcgcct
    25201 cgatcccaac cgcgcccgac ccggcacaca aatctaaaaa tcgagcatcc tcgatacgcg
    25261 gcgcgatgac attgaagagc gtctcgcgca accgatccga cgtcggccgc acattcatcg
    25321 acggcggact cttcaaattc cgccccttgt aaagccctgc aattatcctc atccgatccg
    25381 tgctaatcca aaacgtggat ccgttttaat ccgtgcaacc atcttcgccg tgaccactgc
    25441 cttcggtttc gccagataaa actcagcttc ccgtttcgcc tgctccagta tcacctgatc
    25501 gcgcacaatg tttccgatgc gaaactccgg caatccggat tgcctcgttc ccaacaactc
    25561 gcccggcccg cgcagctcga gatctttctc ggcgatcctg aatccgtcgt ttgtttgcgc
    25621 catgatgccc agacgctcct cggcaactgc ggtcttcttg tcgcttgtta gcagtatgca
    25681 ataactcttc tccgcgccac gcccgacacg cccgcgtaac tggtgcaact gtgacagacc
    25741 aaaacgctcc gcatgctcca cgatcatcac cgacgcattc ggaacgtcaa caccgacctc
    25801 aatcacagtc gtggaaacca gaatcttgat ctcgcccgcg acaaacgcgc tcataacttt
    25861 ctctttctca gccggcttca tcttcccatg caacagaccg accgtgagtt tcggaaaaac
    25921 ggtatcgcgc aggtactcgt aacgacgcgt cgcatccttg agatccatct tctccgactc
    25981 ttcgacgagc gggtaaacga cataaacctg ccggcctgct ttcacttcgc gcgtgatcaa
    26041 acgtttcact tcctggcgct ggtcttcacc aaagacgagg gtcttgatcg gcgtgcggcc
    26101 gggaggcagc tcgtcgatga tcgagacgtc gagatcgccg taaacagtca tcgcgagtga
    26161 acgtggaatc ggcgtcgcgg tcatgaccag cacatccgga ttgagcccac gcgcacgcaa
    26221 ctccgcgcgt tgcatcacgc cgaagcgatg ctgttcgtcg atcacgacga gcccgagctt
    26281 ttcaaacgcg accgcctcct gaatcagcgc ctgcgtgccg atacacgcct gtatcgcgcc
    26341 ttcctttaag gcattttgca ggtgtcgttt ttcagccgcg cgaagcgatc cggtcaacag
    26401 ctcgacacga taaggagttt ttgccagcag gcgcttgatg ttgcgcgcgt gttgctcggc
    26461 gaggatttca gtcggcgcca tgagagcagt ttgcaggttg ttttccatcg ccgccatcat
    26521 cgcgacgacg gcgacgatcg ttttgccgct accgacgtcg ccctgtagca ggcgattcat
    26581 cggagcgttc gactgcatgt cgttgaagat ctgctttgtg acttttacct gcgcactggt
    26641 tagcttgaac ggcattaccg aagcgatgtg agctttaacg gttttgtcga tatgcaacgg
    26701 cgccgctttc acttccttct cgcgttggcc gcgcttcaaa ccgatcgcga acgtcaccca
    26761 gaagaagtct tcgaagatca tccgcaaatg cgcctggctc ttcgactgtt cgtagtcagc
    26821 catcgaaacg tctggcggtg gaaaatgaat ctcacgcacc gcttcagcgc gaccgatcag
    26881 ctttgcgcgg tgtcgcaagt ctgccggaag tgtttcctcg atcgctttgt tattcaggag
    26941 tgagagaacc gcatggacga tttctcggac gcgcttggaa ttaaaatcgc cgagtttacg
    27001 atagaccggc acgcggcgtc cgacgtgaat ggcagcgagt gaaggatcag gctggttttc
    27061 ttcggactgc tcgggatcat cgcctggcgt ttctgaagga ggcgagagta tctcgagctc
    27121 gtcggcagcg ctgtttagtc gcagcttgaa cgtggtgcgg cgtccttccc attcccagcg
    27181 accgaaagtg atgaagcgtg tgccgcgtgt aagcttcgcg gtgtaatact tgacgatgtc
    27241 gtaagcgcgg cggccggaga caaaccacca gacgatcact tcgcgcccgg tcattggacc
    27301 atcgatgccg tagacttcga agatgtaaag ttgtgagcgc ccgtaaccgc gcggatttct
    27361 caccgcgtag ccgtttgttt tggtcacggt gagatcgagt gaggcttcga caccgtcatg
    27421 caggtctttg atcaaagccg gacgggatct atcctcgtag cgcatcggga agtagttgag
    27481 cagatcctcg accgtggctt cggcgaagtt tttcttgtgc gtttgcgtag cgagtgcttt
    27541 agcgagtttt tgagagagaa tcgggcctaa acgagcgatc ttgaaactgt gaagatcctc
    27601 aatcggagtg ttgagggaga tggcgccagg catcgctggg atcataacat agcgagctga
    27661 gagggacggt ttatggacaa cgaacgagca tcggatgaga cgagagataa tttgaaggtt
    27721 ttgcgactgg gcttgttgcg gctgcacaag gcgctgctgg atctcgagcg cagggatttc
    27781 gagcgtgcgc gcgggcggat tgggaatagt tatgagtttt tgcagctagt gctgaaggac
    27841 ccgtggttcg actggttgca tcgtctttcg gagttgatcg tgcagataga tgaaacgctc
    27901 gatccgcatt ctgaaacacc agcgaccgaa gatgatgcga aggcgttaat cgaaagggcg
    27961 aggacgttgc tggcgccgtc ggaaagcggg agtgagtttc agaagaatta ctttctcgcg
    28021 ttgcagcagt cgccggatgt ggtgctgttg cattccgagg tgatcaagtt gttgggaaga
    28081 taatttagcc acggattaca cggataaaca cagatcggtg tttatccgtg taaatctgtg
    28141 gcttaacaat attcttcaaa tgcgcgcgtt aattcccccg cgatctgctg tgcatcgcgt
    28201 ccttcgatct ggtatcgctc gagcatgtag acgagcttcc cgtccttcaa caatgcgatg
    28261 gacggcgatg atggtccata acccgtgaag tagctgcgtg ctctttcggt tgcctcgata
    28321 tcggcgcccg cgaagacggt cgcgacttta tcaggcttga cctcattctg aagcgcaagc
    28381 gccacgccgg gccgcgcctt tcccgccgca caaccacaga tcgaattaac gacgaccatc
    28441 agcgtgcctt tactattctg tatcgtgtcg tcgactgctt ccacagtctt caattcctca
    28501 gctccgagcc gcgtcagatc ctcgcgcatc ggacgtatca tgatttccgg atatggcatt
    28561 ccagtgagtc tccctaaact aatttttgag ttacagatta tacatcaggg tcgagagaac
    28621 ggatggcctc gctgaggtgt tcgcggatgt cggatgccag cattgtgcgc atacccaatc
    28681 ttttagcggc gaaatcacca gccaggccgc cgacgtaaag tgcggcgact gtggccctga
    28741 ttgcattcgc acgatctttg agcgtgccgt aggcttgcgc gaggaagccc gagattaggc
    28801 ccgtgagggt gtcgccggcg ccggccgtgc cgagtccggc gttgccggtc gggttgatga
    28861 atacgcggcc tccgggtgtg gctatgagcg ggcgcgcgcc tttaagaaca aggactacgt
    28921 gatgagcagt cgcaaactcg cgcgccgtcg cgacccgatc gtccagatct gatttatcga
    28981 tcagccgcag catttcgccg ggatgcggag tcaggattaa cgggtgctca tccgagcctt
    29041 tgagggtggc aggccagggc gcgagcgagt tcaggccgtc tgcgtcgatc acgcagggcg
    29101 tagtgcgctg ctcgacgacg ctgcgaacaa agcgacgcgt gcgttcatca tctgacgtca
    29161 ggcctggtcc aatcgccacc acggtcgcct tttccgcgag tttcaaaaag tgatttgtcg
    29221 cctcatcgct gacgacgcca cgatctgttt cggccaacgc cgtcgtgatc acttccggca
    29281 ttgcgctgct cgcaactgat acttgcgccg aagcaggcgt cgcgatcgtc accaaccccg
    29341 cgccggatct catcgccgcg ttgccacaaa gcgctgccgc acccgagtag ccacgcgagc
    29401 cggcggctat caaaacgtgc ccgtgcgtgt ttttgtaaga gtcgggcgtg tagcgagtgc
    29461 tcgcgagcca cgcacgcgcg tcttcggctt cggtgacgaa taagaatggt ttcgcggcag
    29521 cgattaagga agacggcgaa ccgatgtcag ccactatcaa ctcaccgcca aactcgcacg
    29581 cgggcggaag cacattcgcc gttttggccg cggtgaacgt caccgtgaga tcggcctgga
    29641 ctgctggtcc aatcggcttc gcttggtcgg cattgagtcc ggaaggaatg tcgatggaga
    29701 agaagagcgg gcacacgcct gaggctcgtt ctcgcgcctc acgcagcatc gtcaaatgtt
    29761 cgatgacttt gaggtatacg ccctcgagcg gccgtgttag tccggttccg aaaagcgcgt
    29821 caacaatgat gtcgtaggcg cgacgcggct tcgcaatctg ttcccaggcc gcgacgcttt
    29881 cgcattcaat aaacgtcatt ggcggcggcg cttccggtga accggcctcg aagcttgcca
    29941 ggcgcgcgac cgcgtcgaag ttagcgcgcg cgtcgccgga ggtctccgaa agcttgccga
    30001 agagtacgac gctgcagtgc atgcctgcgc gtgacatagc tcgcgcgagg gctgcgccgt
    30061 caccgccgtt attacctttt ccacagagaa tcagggcttt gcggtttgcg agttcgttgt
    30121 cgaggcgtgt gcggatagcc tccagacacg cactgcttgc cgcttccatc agtagcacgg
    30181 aaggcgtttg gtagttctca acggtaagac ggtcgatctc gcggatctgt tcggcggaga
    30241 gtactggtcg cacgtttgtg atgatagcag gaacgcggat gaaggcggct atatgaacgc
    30301 ggatatacgc gcatcagaca gaaaaccagc cagggccgcc cccgcctgcc tgccgctgct
    30361 ccaaccgtcc gtattttcca ggatgtctaa gatatatatt caatggccca gcctctccag
    30421 aaaaatttaa aaaaagattc tggaacaaac acgaggtctg ttccagaatc ttgtaagcct
    30481 tgttttgtaa gttttagatc gtgatcgcag cgcgaacatt gctgcgggcg ttgacctgcg
    30541 tgttctcact cgcgcgcgac aaagcggcgc gcatcatgta catcagggtc tcgcaaacct
    30601 cgcgtgagcc cgttcctaca acctggcctt tttcgtcttc cagcacgtaa atccggccgg
    30661 tccaatcaag gggaagaatg atgggggcac tatacatcgt tcttagtctc ctgcgattcg
    30721 ttagttttct ttctgttttc tgagacggcg gcgaagcgca cctgcggaac cgacgagtcc
    30781 gctaccgagc aggaacaagc tggctggttc ggggacgttg ttggtggcgg gggcaaatcc
    30841 accgagtcgc cattgattaa tatcctgaaa cgtcgttcca ttcaag
//
LOCUS       DASSFQ010000213         6015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1428499, whole genome shotgun sequence.
ACCESSION   DASSFQ010000213 DASSFQ010000000
VERSION     DASSFQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428499"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFH96_07815"
     CDS             complement(<1..250)
                     /locus_tag="VFH96_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET6973916.1"
                     /translation="MSLTPLPRFLLPTRARIRIVLMAVLAAIAVGTIVFHLVEGWSIL
                     DSLYVTVQTLTTVGFGDITPRTTLGRAFATGFMMVGVGV"
     gene            515..3169
                     /locus_tag="VFH96_07820"
     CDS             515..3169
                     /locus_tag="VFH96_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET6973917.1"
                     /translation="MSYVESRPSFVRRVLRRLFSAPVLIPLVFVTAIVLGILIYYWTV
                     FSRRIDNLLAGEVFTRTAGIYAAPKQLHVNETISQADVINFLKRAGYVEKTQQADESR
                     GRYAANGTSLDIEPSSASSVDGARQFQNVRVQFSGSGKSISALTDLDARGSLQRVWLE
                     PELISSVTGTERAKRKVIGFSDLPPHLVKAITVTEDRSFFEHYGVNVRGIIRALIRRY
                     DSDPNSPIARQGGSSITQQLVKNLLLSPEQTWQRKISEAYMSIILETRLSKEKIFELY
                     CNQVYMGQQAGFSINGFGEASSAYFNKDVTNLTLPESAFLAALIRSPNRYNPYTKIET
                     AAARRNQVLDSMAEAGAITQPEATTAKATPLQVVPARGRIDVSDAPYFADYVQNQLGD
                     MIAGNGGTEHLRIYTTIDMDLQRAAYAALTKQMNALDKIQSKRFAPGTLQAALVAMNA
                     KTGEIVAMVGGRDYSKSQLNRATDAYRQPGSVFKPFVYATALNTAFDPVPRVITPATT
                     YMDEPKTFTFDNQEYSPGNFGETYTHEPVTLRDGLVHSLNVVTVEVAMEVTIGRVMNL
                     AAKAGLPKPPRAYPAMALGTSEATPLQIASAYTAFANLGSRTTPIAINRVTTGEGATI
                     AAPTTQRSEVLRPEVAFVMTSFMKDVVNRGTAAKVRSRGLKQVLAGKTGTSRDGWFAG
                     YTPNLVCAVWVGFDDGSQLGLTGANSALPIWANFMQVALAEHPEWEGDWDMPPGVEQV
                     EINPKTGTPTAAGDTEKRTEFFINGTGPNDTSETPNEEATPVELPSPSPIEPPYQEAP
                     IPLASPSPSPRRAPALPDSGLEGTITLDIDPQTGLIAVESCPVIRTKTFVLGTEPKKY
                     CGPQYHQRNIIEPAPAARPRVVGTPQHR"
     gene            complement(3166..3522)
                     /locus_tag="VFH96_07825"
     CDS             complement(3166..3522)
                     /locus_tag="VFH96_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET6973918.1"
                     /translation="MSYSAKFKDHLAHPRNAGELSDANVVADETNPICGDRLRLSLIV
                     KDDRIEAVRYLAYGCPPTLVCGSVLTELIGGKTIGEVRKLTRADLLQEIGGLPSRKHH
                     AAALAIETLNSALRDF"
     gene            complement(3544..4728)
                     /locus_tag="VFH96_07830"
     CDS             complement(3544..4728)
                     /locus_tag="VFH96_07830"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET6973919.1"
                     /translation="MPARRVYLDNSATTPVDPRVAAAMARAVVENFGNASSVHGFGQQ
                     ARAAVDRARREVAALVGAKQNEIVFTSGGTEANNLAIRGLCEAAATHGRHIITSSIEH
                     PSVTGVCAEMEKRGWEVTRLPVYQNGIVRVEDVATALRPETVLITIMMANNEIGTIQP
                     IAEIGALVREKRAAGHKYLWFHTDAVQAAGRLRLHVDELGCDLLTMSAHKLYAPKGIG
                     ALFVRRGVRLHGQNVGGHQERERRAGTENVPGIVAFGEAAKLAREELEARKVHDLALR
                     DRFENAVAERIPDIVMNGDRERRLGHLSNISFRFIEGEGLLINLDLEGVAVSTGSACS
                     SGTLEPSPVIRALGVNDELARGSIRFSFGKDNTEDEIEYVVEVLVRAVERLRALSPLT
                     LV"
     gene            4899..5891
                     /locus_tag="VFH96_07835"
     CDS             4899..5891
                     /locus_tag="VFH96_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973920.1"
                     /translation="MNYLKLPLVVLLSICVSVLLAIVPAGAQGTTALERGYRTGYSDG
                     YTAGYSDVSNNAPRNYQNKEDYQKADRSYNDVWGPVEDYRDGYQQGFESGYTAGYERQ
                     QFNSALPTGLHRRGANSSSGPANGGAAPPEVNPQPNNDPVDVNATTAPNGSLAIPRNT
                     ILALELLTPVSSDVSQRGDRIQARVFEPAQFAGYIVEGRVAQVKRAGKVKGVAELQLS
                     FDQIRSPDNRSAALHAELVEISPIGSSDPQIDSEGGVKGQDSTKDDVTKVGAASGIGA
                     IIGAIAGGGKGAAIGAIIGGGAGTAGVMTQRGKDIRLEHGQHIKIRTSTDTGVL"
BASE COUNT         1386 a         1725 c         1669 g         1235 t
ORIGIN      
        1 caacgccgac gccgaccatc atgaacccgg tcgcgaaggc gcggcccagc gtggtgcgcg
       61 gggtaatgtc gccgaatccg accgtggtga gcgtctgcac ggtaacataa agactgtcaa
      121 gtatagacca gccttccacg aggtgaaata cgatggttcc gaccgcgatc gcagccagaa
      181 ccgccatcaa aacgatacgg atgcgagcac gggtggggag aaggaaacgc ggcaggggtg
      241 ttagactcat acaggccgag tggcatcaaa gtcaccagcg agggcagcca cgttaacgaa
      301 tgcggccagc cctgtcaaac ttaatgtcga cagaaccgct ggagggatgt tacgtaatag
      361 tgagacagac gtttttatcc acagattaca cagagcttta aaataatctg cgtaatctgt
      421 ggatagataa attgaacctt ttatgccagt gcgctatcca ccagataatc aatatccccg
      481 cacaaccaat cgtgatctca aaaagccgcg cgtcgtgtct tacgtggaat cgcgtccgag
      541 ctttgtgcgg cgagtgctgc gacggttgtt cagcgcgccg gtattgatcc cgctggtgtt
      601 tgtcacggct atcgtactcg ggattctgat ctactactgg acagtcttct cgcgtcgcat
      661 cgataacctg ctcgcgggcg aagttttcac acgcaccgcc ggcatctacg ccgctccgaa
      721 acaactgcac gtcaacgaga cgatctcgca ggccgacgtg attaattttt tgaagcgtgc
      781 gggctacgtc gagaagacgc aacaggcaga cgaatcgcga ggtcgttatg cagcgaacgg
      841 aacatcgctc gatatagagc cgagcagcgc ttcgagtgtt gatggtgcga gacaatttca
      901 gaatgtgcgt gttcagttta gtggttccgg gaagtcgatt agcgcgctca cggacctcga
      961 cgctcgcgga tcgttgcaac gcgtgtggct cgagcccgaa ttgatcagct cagttaccgg
     1021 caccgagcgc gcgaaaagaa aagtcatcgg cttcagcgat ctaccgccac accttgtcaa
     1081 agcgattacc gtcacagaag atcgctcgtt cttcgagcac tacggcgtaa acgtgcgcgg
     1141 catcattcgc gctctcattc gcagatacga ttcggatccg aattcgccga tcgctcgcca
     1201 gggtggttcg agtatcacgc agcagctcgt gaagaacctt ttgctctcgc cggaacagac
     1261 gtggcaacgc aagatcagcg aagcgtacat gtcgatcatt ctcgagacgc ggctgagtaa
     1321 ggaaaaaatc tttgagcttt actgcaatca ggtttacatg ggtcagcagg ctggattctc
     1381 gatcaacggt tttggcgagg cttcgagtgc gtacttcaac aaggacgtta cgaacctcac
     1441 gcttccggaa tcggcttttc tcgcggcatt gattcgcagc ccgaatcgct ataacccgta
     1501 cacgaagatc gagactgcag ccgcgcgtcg caatcaagtg ctggactcga tggccgaagc
     1561 cggcgcgatc acgcagccag aagcaacgac tgcgaaagcg acgccgcttc aggttgttcc
     1621 cgcgcgcggt cgcatcgacg tctctgacgc accgtacttc gccgattacg ttcagaacca
     1681 gctcggcgac atgatcgcgg gcaacggcgg caccgaacat ttgcggatct acacgacgat
     1741 cgacatggat ctccagcgcg ctgcatatgc cgcattgacg aagcagatga atgcgctcga
     1801 caagatccag tcaaaacgtt ttgcgccggg tacgttgcag gcagcgctcg tcgcgatgaa
     1861 tgcgaagacg ggcgagatcg tcgctatggt tggcggtcgt gattattcga agagccagtt
     1921 gaatcgagct actgatgctt atcgacagcc agggtctgtc ttcaaaccat tcgtctacgc
     1981 gacggcgcta aataccgctt tcgatcccgt gccgcgcgtg atcacgccgg ccacgactta
     2041 catggacgag ccgaagacgt ttacgttcga taatcaggaa tattcgccgg gaaactttgg
     2101 cgagacttac acgcacgagc cggtcacgtt acgcgatggg ttggtccata gtcttaacgt
     2161 cgtcacggtt gaagtggcga tggaagtgac gattgggcgc gtgatgaatc tagcggcgaa
     2221 ggcgggattg ccgaaaccgc cgcgcgcata tccagcgatg gcgctcggta cgagtgaagc
     2281 aacgccgctg cagatcgctt ccgcctacac ggcgtttgct aaccttggtt cgcgcacgac
     2341 tccgatcgca atcaatcgcg taacaacggg tgagggcgcc acgatcgcag cgccgacgac
     2401 gcaaaggagc gaagttcttc gtccggaagt cgcattcgtg atgacctcgt ttatgaaaga
     2461 cgtggtcaat cgtggtactg ccgcgaaagt gcgcagtcgc ggtttgaaac aagtgctcgc
     2521 cgggaagacc ggaacatcgc gcgacggatg gtttgctggt tacacgccga accttgtttg
     2581 cgcggtgtgg gttggtttcg acgacggctc gcaactcggt ttgaccgggg ccaattccgc
     2641 cctgcctatc tgggccaact tcatgcaagt cgcactggcg gagcatcccg agtgggaagg
     2701 cgattgggac atgccaccgg gtgtcgaaca ggttgagatc aatccgaaga ccgggacgcc
     2761 gacggcagcg ggcgatacgg agaaacgaac ggagtttttc atcaacggca ctggtccaaa
     2821 cgacacctcc gagacgccga acgaggaagc aacaccggtt gaattgcctt cgccgtcacc
     2881 gatcgaaccg ccatatcagg aagcgccgat acccttagct tctccgtcac catcgccgcg
     2941 tcgtgcgccg gcgttgccag acagcggatt agagggaacg atcacgctgg acatcgatcc
     3001 gcagaccggt ttgattgcgg tcgagagttg tccggtgatt cgcacgaaga cgtttgtgct
     3061 cggtacagag ccgaagaagt attgcgggcc tcaatatcac cagcgcaaca tcatcgaacc
     3121 agcacccgca gcgaggccgc gcgtcgtcgg cacgccacaa catcgttaga agtctcgtaa
     3181 agcggagttg agtgtttcga tggcgagtgc ggccgcgtgg tgtttgcgag aaggcagacc
     3241 accaatttct tgcaacagat cggcccgcgt taactttctg acttcaccaa ttgtctttcc
     3301 accgatcaat tcggtcagca ccgatccaca aacaagcgtc ggcggacagc cgtaagcgag
     3361 atatctcact gcctcaattc gatcgtcttt aacaatcaat gacagccgca accgatcccc
     3421 gcaaatcgga ttcgtctcgt ctgcgacgac attcgcatcc gataactcac ccgcgttacg
     3481 cggatgcgcc agatgatctt taaactttgc ggagtaagac aagaatccgt gtgtaatccg
     3541 tggctaaact aacgtcagtg gcgacagcgc tcgcaatctt tcaacggctc tgacgagcac
     3601 ctcaactacg tactcaatct catcctcggt attatctttc ccgaaactaa accgtattga
     3661 tccacgcgcg agctcgtcat taaccccaag cgcgcgaatc accggcgacg gctccaacgt
     3721 tcctgacgaa caggccgaac cagtcgacac cgcaacccct tcgagatcga gattgatcag
     3781 cagcccttcg ccttcaatga agcgaaacga gatattcgag agatggccca ggcgccgctc
     3841 gcgatcaccg ttcataacta tgtcagggat acgttccgcg acagcattct caaatctatc
     3901 ccgcaacgcc aaatcgtgaa cctttcgcgc ctcgagttct tcgcgtgcga gtttcgcggc
     3961 ctcaccaaac gcgacgatgc ccggtacgtt ctctgtgccc gcgcgtcgct cacgctcctg
     4021 atgcccaccg acattctgac cgtgcaagcg aacaccgcgc cgaacgaaaa gcgcaccaat
     4081 acctttcggc gcatacaact tgtgcgctga catcgtcagc agatcacaac caagctcgtc
     4141 aacatgcaaa cgcaaccgtc ccgcggcttg cactgcgtcc gtgtgaaacc aaagatattt
     4201 gtgtcccgct gctcgtttct cacgcaccag cgcgcctatc tcagcgattg gttgaatcgt
     4261 cccgatctcg ttattcgcca tcatgatcgt gatcaggacc gtctcgggac gcagcgccgt
     4321 cgccacgtct tcaaccctca cgatgccatt ttgatatacc ggcagtcgcg tgacttccca
     4381 gccgcgtttt tccatctcgg cacaaacacc agtaactgac ggatgctcga tcgacgaagt
     4441 gataatgtgc cggccgtgag tcgctgctgc ttcacaaagc ccgcgaatag cgaggttgtt
     4501 tgcctcagtt ccacctgaag tgaacacgat ctcgttctgc tttgcgccaa ccagcgccgc
     4561 gacctcgcgt cgcgcccgat cgaccgccgc ccgcgcctgt tgcccgaagc catgaacact
     4621 ggacgcattc ccaaaattct cgaccaccgc gcgcgccatc gccgccgcga cgcgcgggtc
     4681 gacgggcgtt gtggcgctgt tatcaaggta gactcgacgt gctggcatca aattcaactc
     4741 cacccgcatc tgaatcgcag tttgccataa aggcgcgcaa aaagcacaaa atgtgcccct
     4801 ttttcggcaa ttgatgatac atttcaaacg ctgcgaggtt ggctctccca agacgctacg
     4861 atcattcccg ggtcgtaaat tcttcggaag gacaaaatat gaattatctt aaacttcccc
     4921 tggtggtttt attgagcatc tgcgtgagcg tgctgctggc catcgttcct gccggagcgc
     4981 agggaacgac agcgctcgag cgcggttatc gcactggtta ttcagacggt tacacagccg
     5041 gttatagcga cgtctccaac aacgcgcctc gtaactatca aaataaagag gattatcaaa
     5101 aggccgatcg ttcttacaac gacgtttggg gaccggtcga ggattatcgc gacggttacc
     5161 agcagggatt cgaatccggt tacacagcag gctacgagcg ccagcagttt aactcggcat
     5221 taccaacagg actgcatcgc cgcggagcaa actccagcag tggtcctgcc aatggtggtg
     5281 cggctccgcc ggaagttaac ccgcagccga acaacgatcc ggttgatgtg aatgcgacaa
     5341 cggcgccaaa cggctcactc gctattccac gcaacacgat tctcgcactc gagctcctga
     5401 cgccggtctc gagcgatgtg agccagcgtg gtgatcgaat tcaggcgaga gtcttcgagc
     5461 cggcgcaatt cgctggttac atcgttgaag gacgagttgc gcaggtaaag cgggctggaa
     5521 aagtgaaggg cgttgctgag cttcagttgt cgtttgatca gattcgatca cccgacaatc
     5581 gcagtgcggc gctccacgcg gagttggtcg agataagtcc gattggcagc agcgatccgc
     5641 aaatcgattc agaaggtggt gtgaagggcc aggactcgac gaaagacgat gtgactaaag
     5701 tgggcgcagc gtccggcatc ggcgcgatca tcggtgcgat tgcgggcggc ggcaaaggcg
     5761 cagcgatcgg cgcgatcatc ggcggcggcg caggtacggc cggcgtgatg acgcaacgcg
     5821 gtaaagacat tcgtctcgag cacgggcagc acataaagat tcgcacctcg actgatacag
     5881 gcgttctgtg aatcgcgtta ttactttgct caccgatttc gggacggctg attacttcgt
     5941 cggtgcagtg aagggcgcga ttctcgcggt taatccgcga gcggtgattg tcgatatcac
     6001 tcacgagatc gcgcc
//
LOCUS       DASSFQ010000214         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1587644, whole genome shotgun sequence.
ACCESSION   DASSFQ010000214 DASSFQ010000000
VERSION     DASSFQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587644"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..824)
                     /locus_tag="VFH96_07840"
     CDS             complement(273..824)
                     /locus_tag="VFH96_07840"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET6973921.1"
                     /translation="MKKRCFWARTPLSIAYHDEEWGVPLHDDNRLFEFLILEGAQAGL
                     SWETILRKRDNYRKAFDRFNPAKVAKYDQKKIDQLLNNDGIIRNRLKINSAVTNARAF
                     LEVQREFGSFAEYVWRFVNGKPLKRKRGQPIAATTEISDALSKDLLKRGFKFVGSTIC
                     YSFMQAVGMVNDHDATCFRYRQV"
     gene            complement(901..2505)
                     /locus_tag="VFH96_07845"
     CDS             complement(901..2505)
                     /locus_tag="VFH96_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973922.1"
                     /translation="MSQEAYSRREAQAEPAARPVELHSDLYREIFAHSKEAIAIIDPD
                     GYYLQQNGAHFILLGYADNDLEGKTPAIYLGEETFQKILSELVRNGDFAGETICRTKN
                     GEERTIELSVFTMRSGLGEPQCYVSIKRDITTRKKTEERLAQLLVRERTARADAEKAN
                     RLKNEFLATLSHELRTPLNAVIGWSRILKAGRLDNESSVHAIEVIERNAWAQKQIIED
                     ILDVSRVITGKLQLHLGPVDLISVVNAALDAVRPALEAKDIRIETQFQKGLKVIAGDV
                     DRLQQVVWNLLSNASKFTPVGGTVGVRINQDETYAQIQVRDTGPGIAADFLPHVFERF
                     RQADGSTTRTHGGLGLGLAIVRHLVELHGGMIAAENSGSGAVFTVRLPLPSSELSLER
                     LPAPARVDQTVTEVDLKDVRILVVEDELDALDLLTIDLTAHGARVSGAASAAEAIELL
                     RTEQFDLMISDIGMADTDGYNLIKQVRRQEGEHGEHIPAIALTAYARTQDRIRALTAG
                     YNTHVAKPVETKELVTVVKCLIGKIG"
     gene            complement(2569..3555)
                     /locus_tag="VFH96_07850"
     CDS             complement(2569..3555)
                     /locus_tag="VFH96_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6973923.1"
                     /translation="MAKIKIGIMGAGYIAGVHASVLASDDRTQLTAVYDVDEHAAVKL
                     AGAHNATAVATTLELLERCDAVYITTPNTQHVSLAIASIETGKHVFCEKPLATNIADA
                     ERVFKKASDARGVFQVGHNRRFAPVYATLKRMLSETHRPHSAHVKMNRGELLKPEWTG
                     DPSVTGGFLYETPIHMFDMMRFLFGEIEALHAIGSTHKYKEVDDFSVLLKFASGMHAT
                     LATAADASWMFPFERVEVFAHHATLVTREMESLTYSTALEGNFTEQTMQQLPREEKWG
                     YAQEDRAFIDAIVNGSEPLVTAFDGLMSVRLVDAVYESIRSIAPVTIPSVSA"
BASE COUNT          759 a         1016 c          994 g          846 t
ORIGIN      
        1 tcagccggcg acatcacgcg cggcgcggct tcgttaagta tgcgttgcgg actcacgtag
       61 atgaccgcgt cggcttcagg gagactggcg agtgcggcgg cggagttctg ggcaatcgcg
      121 gcgctcgtta gtgaaagaac agcgagaaga gcaagtggca gaagtttgaa tcgcaaattg
      181 agggacctcc agtggcgaaa agatgcagga taatttctca caaagaactt ctggtggaaa
      241 ggtatttctc tgtagagatc gcagagaaga agttagacct gacggtaacg gaaacaggtc
      301 gcgtcgtggt cgttaaccat tccgaccgcc tgcatgaacg aataacagat cgtcgacccg
      361 acaaacttga acccgcgttt gagcaaatct ttgctgagcg cgtcggagat ctcggtagtt
      421 gcggcaatcg gctggccgcg cttacgcttc agcggtttgc cgtttacaaa ccgccaaacg
      481 tattcagcga agctgccaaa ctcccgttgc acttcgagaa acgcccgggc attggtcaca
      541 gcggagttga tcttgagtcg attgcgaatg atgccgtcgt tgtttagtaa ctggtcaatc
      601 ttcttttgat cgtattttgc gactttcgct ggattgaaac gatcgaaggc tttgcggtag
      661 ttgtcgcgct tgcggagaat agtttcccaa ctaagaccgg cctgcgcgcc ttcgagtatc
      721 agaaattcga acaggcgatt gtcatcgtgg agcggcacac cccactcttc gtcgtgatac
      781 gcgattgata ggggcgtcct ggcccagaag caacgctttt tcatgcagca gtcgtcagac
      841 aggatttcag gatttacaag attatcatga taatcctgaa aatcttgtga atcctgttct
      901 ctaaccgatc ttcccgatca ggcacttaac caccgtcacg agctctttag tctccaccgg
      961 cttcgcgacg tgcgtgttgt aacccgccgt gagcgcgcgt atccggtcct gcgtgcgcgc
     1021 ataagcagtc agcgcgattg ccggaatgtg ctcaccgtgt tccccttcct gtctgcgcac
     1081 ctgcttgatc agattgtaac catccgtatc cgccatgccg atgtcgctga tcatcagatc
     1141 aaactgttcc gtacgaagca actcgatcgc ttccgctgcc gacgccgcgc cacttactct
     1201 cgcaccgtgt gccgtgaggt caatcgtcag cagatcgagc gcatccaact cgtcttcaac
     1261 cacgagaatc cgcacgtctt tcagatcgac ttcagttacc gtttgatcta cacgcgccgg
     1321 tgcgggcaaa cgctccagac tcaactcact gctcggcagc ggaagtctca ccgtaaaaac
     1381 cgcgcctgaa ccggaatttt ccgcggcaat catcccgcca tgcagctcga ccaggtgacg
     1441 cacgatcgcg agtccgagtc cgagaccacc gtgcgtgcga gtagtcgatc cgtctgcttg
     1501 cctgaagcgc tcgaacacgt gcggcagaaa gtcggccgca atgcctggtc ctgtgtcgcg
     1561 cacctggatc tgcgcgtaag tttcatcctg attgatgcga acaccaaccg ttccgccaac
     1621 tggagtaaac ttcgaagcat tcgaaagcag gttccaaacg acctgttgca gtcgatcgac
     1681 gtcgcccgcg atcactttga ggcctttctg aaactgcgtc tcgatgcgga tgtcctttgc
     1741 ttcgagcgcc ggtctgactg catcgagcgc tgcattcaca actgagatca gatcgactgg
     1801 tccaaggtgt agctgaagct tgcccgtgat gacgcgcgac acgtccagaa tgtcttcgat
     1861 gatctgtttc tgcgcccacg cgtttctctc gatcacctcg atcgcgtgaa ccgagctttc
     1921 attgtcgagg cgcccggctt tcagaattcg cgaccaaccg atcacggcgt tcagcggtgt
     1981 gcgcaactcg tgcgagagcg tcgcgagaaa ctcattttta agtcgatttg ctttttcagc
     2041 atcggcgcgc gccgtgcgtt cgcgcacgag cagttgggcc aacctctcct cggtcttctt
     2101 gcgtgtggtg atatcgcgct tgatgctgac gtaacactgc ggctcgccca ggccgctgcg
     2161 catcgtgaac accgagagct cgatcgtccg ttcttcaccg ttcttcgtgc gacagatcgt
     2221 ctcgcctgcg aagtctccat tccttacgag ctcgctgaga atcttctgga aagtttcttc
     2281 accgagatag atcgcgggag ttttgccttc gagatcgttg tcggcgtagc caaggaggat
     2341 gaagtgtgcg ccgttctgtt ggaggtaata gccgtcggga tcgatgatgg cgatcgcttc
     2401 ttttgagtgc gcgaagatct ctcggtagag atcggagtgc aactcgacag gacgagcggc
     2461 cggctcggcc tgagcttctc gacgtgaata ggcttcttgc gacatacaaa cgcctaaact
     2521 ttgaagggga ctgggccggg ggactcgata ttaacactaa accgcggatt acgcggaaac
     2581 actggggatt gtcacgggag ctattgagcg tatggattca tacaccgcgt ccaccaaccg
     2641 caccgacatg agaccatcaa acgccgtcac gagaggctcg gatccgttaa cgatcgcgtc
     2701 gatgaacgcg cggtcttcct gcgcgtagcc ccacttttct tcacgcggca gttgctgcat
     2761 cgtttgttcg gtgaagttac cttccagcgc ggtgctgtaa gtcagactct ccatctcgcg
     2821 cgtcacgagc gtcgcatgat gcgcgaaaac ttccacacgc tcgaatggaa acatccagct
     2881 cgcatccgcc gccgtggcga gcgtcgcgtg catcccgctc gcgaatttca gaagcaccga
     2941 gaagtcgtct acctctttgt acttgtgcgt cgaaccaata gcgtgtaatg cctcaatctc
     3001 gccgaagaga aaccgcatca tgtcgaacat gtgaatcggt gtctcgtaaa gaaagccgcc
     3061 cgtgacagac ggatcgccgg tccattccgg tttgagcaac tcgccgcgat tcatcttcac
     3121 atgcgcggag tgcgggcggt gtgtttcgga gagcatgcgt tttagtgttg cgtaaacggg
     3181 cgcgaagcgg cgattgtggc ccacttggaa tacgccacga gcatcacttg ctttcttaaa
     3241 tactcgctct gcgtcggcta tattcgtggc gagtggcttc tcgcaaaaga cgtgtttgcc
     3301 ggtctcgatc gaagcgattg ccagcgagac gtgttgcgtg tttggagtgg tgatatagac
     3361 cgcgtcgcat ctttcgagga gctcgagggt ggtagcaaca gcggtggcgt tgtgtgcccc
     3421 agctaatttt acagccgcat gttcatcaac atcatagact gccgtgagct gcgtgcggtc
     3481 gtcgctcgcg agcacactcg catgaactcc ggcgatgtaa cccgcgccca tgatcccgat
     3541 tttgatctta gccatataca cctccaaagg atacaactgt aggggtggcc ctccgtggcc
     3601 acccgtttca ttgaa
//
LOCUS       DASSFQ010000215         3514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_248435, whole genome shotgun sequence.
ACCESSION   DASSFQ010000215 DASSFQ010000000
VERSION     DASSFQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248435"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VFH96_07855"
     CDS             <1..1209
                     /locus_tag="VFH96_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET6973924.1"
                     /translation="AAAARLVPTVDEIGEQFGIRVANKRISITPVSLITEGLSGDVVQ
                     VARAIDDAAGTVGVDFIGGYSALVQKGATPSESRFLDSIPEALSSTKCLCSSVNAATT
                     RAGINMDVVGQIGRIIKDAAERTRDRGGLACAKFVCFANAVEDNPFMAGAFHGVSEAE
                     FVVNVGVSGPGVVHHAVRHADRNLPLQDLAETIKKLSFKLARAGELVGREAARRLGIP
                     FGIIDLSLAPTPVPGDSVANIIEAMGFERAGTHGTTAALALITDAVKKGGAMASGSVG
                     GMSGAFIPVSEDAGMIEATQLGALSLAKLEAWTSVCSVGIDMVAVPGSTTAETIAAII
                     ADEMAVGVMNNKTTAVRIIPVPGRDVGDMVEFGGLLGSAPIMPVSEFSSAGFIRRGGR
                     IPAPIHSLRN"
     gene            1271..1594
                     /locus_tag="VFH96_07860"
     CDS             1271..1594
                     /locus_tag="VFH96_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HET6973925.1"
                     /translation="MPEALGMVECRGLVAMIEAADAMVKAANVRLVGWEKIDAGLVTA
                     IVRGDVAAVRSAVDAGAAAAKRVGEVVATHVIPQPHSDVDLGLPVLHTGETTRKKISG
                     SVRGK"
     gene            complement(1595..2860)
                     /locus_tag="VFH96_07865"
     CDS             complement(1595..2860)
                     /locus_tag="VFH96_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973926.1"
                     /translation="MFSEQNLRRTVVIAVCGLVLSGLIFGEGVFAFVSVSQDRPRSTQ
                     TPAATPTPPATVPAQPTSSPTPTPQTQKPQIDRIAPTLGEPPPPPKLKPTPTPTPPEE
                     FDKESIIKIDSELVQLHVRVIDRNNRPINNVPQGDFHVFEDGVAQPIESFTREEVPIS
                     YGLAVDTSASLRSQIQSVIDAGKTIVNSNKPGDETFLVRFISSDKIQTEQDFTDKKDL
                     LIDALENLYVEGGQTAVIDAVYLSAEHVSEYKKNDDSDRRRRALIVITDGEDRSSFYK
                     QEQLFARLREEDVQIFVIGFVNELDKEAGFIRKSPREKAVNLINKLASETGGRAFFPE
                     SISELPQIANEIVRDMRTQYVIAYNPTNKAQDGSYRAIKVTVEQPSDSEKRIALTRNG
                     RLARKDTGASSVRPPAVRLPATSTPVKKP"
     gene            complement(2989..>3514)
                     /locus_tag="VFH96_07870"
     CDS             complement(2989..>3514)
                     /locus_tag="VFH96_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6973927.1"
                     /translation="DGDSILNKLTFVETKSQTALYDACYLGVEKVQRGAHPKRALLLI
                     SDGQDNDSRYTFNELRKLLKESDVTLYAIGILSGSDAGSAMGMEGQGILDELSGVSGG
                     KAFFPRSAAEMDDIFEQIALELRHQYSIGYKPDTFANDGKWHRVKVKVTPPRGLPRLF
                     VRSKEGYYATAGAR"
BASE COUNT          719 a          941 c         1030 g          824 t
ORIGIN      
        1 gccgctgctg ctcgccttgt ccccaccgtc gacgagatcg gcgaacagtt tgggattaga
       61 gtcgcgaaca agcgcatttc gatcacgccg gtttcgctga tcaccgaagg attatcagga
      121 gacgtggtcc aggttgcgcg cgcgatcgac gatgcggcgg gcactgtcgg tgtcgatttt
      181 atcggcggct attcagcgct agtccagaaa ggcgccacgc cttccgagtc cagatttctc
      241 gactcgattc ccgaagcgct ctcgagcacg aagtgtctct gttcttcagt caacgccgct
      301 acgactcgcg ccggtatcaa tatggatgtg gtgggccaga tcggtcgtat catcaaagac
      361 gccgccgagc gaacgcgcga tcggggcgga ctggcctgcg caaagtttgt ctgttttgca
      421 aatgccgttg aagacaatcc cttcatggcg ggcgcgtttc atggagttag tgaagctgag
      481 tttgtggtca atgtcggtgt gtcggggccg ggcgtggtcc atcacgcggt gagacacgcg
      541 gaccgtaatc tgccgttaca agatctcgcc gagacgatca aaaaactctc gttcaagctc
      601 gcgcgcgcag gcgagctcgt gggacgtgaa gccgcgcgtc gtcttggcat tcctttcggc
      661 atcatcgatc tctcgctcgc tcctacgccg gttccgggtg actcggtcgc gaacatcatt
      721 gaagcgatgg ggtttgagcg tgccggtaca cacggaacga ctgcggcgct cgcgcttata
      781 actgacgccg tcaagaaagg tggcgcgatg gccagcggct cggtcggtgg aatgagtgga
      841 gcgtttattc cggtttcgga agacgcggga atgattgaag ccacgcagct cggagcgttg
      901 tctctggcca agctcgaagc atggaccagc gtttgttcgg tcggaatcga catggtggct
      961 gtgcccggct cgacgactgc ggaaactatc gcggccatca tcgcggacga gatggccgtg
     1021 ggggtaatga acaacaagac aaccgcggta cggatcatcc cggtgcccgg tcgtgacgtt
     1081 ggcgatatgg ttgagtttgg tggcctgctg ggaagcgcgc cgatcatgcc ggtcagcgaa
     1141 ttttcgtcgg ccggttttat ccgtcgcggc ggtcgcatcc ccgcccccat ccactcatta
     1201 agaaactagc cacaaaaaat ttcttgtgcc ttttgtgcct ttttgtggct aatattcggt
     1261 ggaggataag atgcctgaag cactcggaat ggtcgaatgt cgtggcctcg tggcaatgat
     1321 tgaagccgct gacgcaatgg tgaaagctgc aaatgtgcgc ctcgtcggtt gggagaagat
     1381 cgacgcgggt ctagttacgg cgatcgttcg tggcgacgtt gcggcagttc ggagtgctgt
     1441 cgatgcgggc gccgcggctg ccaaacgagt cggcgaggtc gtcgcaacgc acgtgatccc
     1501 gcagcctcat tccgacgtcg atctcggttt acctgttttg cataccggcg agacgacgcg
     1561 taaaaagatc agcggttcag tcagaggcaa gtagctacgg ttttttgaca ggtgtgctgg
     1621 tagccggcaa tctcactgcc ggtggtctaa cagaagaggc gccggtgtcc ttacgcgcta
     1681 aacgtccgtt tctcgtcagt gcaattcgtt tctctgaatc ggaaggctgt tccaccgtaa
     1741 ccttgatcgc tcgataggat ccatcctgag ctttgttggt cggattgtag gcaatcacat
     1801 attgcgtgcg catgtctcgc acgatttcat tcgcaatctg aggcagttcg gagattgatt
     1861 cggggaagaa cgcgcgtccg ccggtttcgc tcgcgagctt gttgatcaga ttgacagctt
     1921 tctcgcgcgg gctcttgcga ataaatccag cttccttgtc gagctcgttg acgaagccaa
     1981 tcacaaagat ctgcacatct tcttcgcgca agcgcgcaaa aagctgttct tgtttgtaga
     2041 aactgcttct gtcttcaccg tcggtgatca caatcaacgc gcgtcgccgg cgatcgctgt
     2101 catcattttt cttgtactcg gaaacgtgct cggccgaaag ataaaccgcg tcaataactg
     2161 ccgtctggcc gccttcgaca taaagatttt cgagagcatc aatcaataga tctttcttgt
     2221 cggtgaaatc ctgctccgtt tgaatcttgt cgctgctgat gaaacgcacg agaaaagttt
     2281 catcgcctgg cttgttgctg ttgacgatgg ttttgccggc gtcgataacg ctttggatct
     2341 gcgaacgaag cgaggcggaa gtgtcgacgg cgagaccgta actgatcggc acctcttcgc
     2401 gcgtgaacga ttcaatcggt tgcgccacac cgtcttcaaa aacgtggaag tcgccttgcg
     2461 ggacgttatt aatcggtcga ttgtttcgat cgatgacgcg aacgtgcaac tggaccaact
     2521 cagagtcgat cttgatgata ctttccttat cgaactcttc gggtggagtt ggcgtcggag
     2581 ttggttttaa cttcggtggc ggcggtggct cgcccagtgt cggcgcaatg cgatcaattt
     2641 gcggtttttg agtttgcgga gtcggcgtcg gagacgaagt cggttgagcc ggaacggtcg
     2701 cgggtggtgt cggcgtggcc gcgggagttt gcgtgctgcg tggccgatcc tgactaacac
     2761 ttacaaacgc aaacacaccc tcgccaaaaa tcaatcccga gagtactaaa ccgcagaccg
     2821 cgatgactac ggtccgcctg agattctgct cactaaacaa tctaaaactc ctcaaaacgc
     2881 gcaaggctgg ggctaacagc gcggctgatt aactaaagcc gccaaacgca caccttcaaa
     2941 agatgccccg taagtgagcc tgcgttgccg agagcccttt cttgaaccct aacgcgcgcc
     3001 agccgtggcg taatagcctt ccttggagcg aacaaacaag cgcggcagtc cgcgtggcgg
     3061 cgtcactttc accttcaccc gatgccattt gccgtcgttg gcgaaggtat ccggtttgta
     3121 accgatcgag tactggtgcc gcaactccaa agcaatctgc tcaaagatgt cgtccatttc
     3181 ggctgcggat cgaggaaaga acgctttgcc tccggaaaca cccgaaagct cgtcgagaat
     3241 accctgaccc tccatgccca tcgcgctgcc ggcgtcgctg ccgctgagaa tcccgatggc
     3301 gtaaagggtg acgtccgatt ctttcaaaag cttccgcaat tcgttgaaag tgtaccgaga
     3361 atcgttgtcc tgtccgtcgc tgatgagaag cagcgctcgc ttgggatgtg cgccccgttg
     3421 aactttttca acgccgagat agcaggcgtc gtagagcgcg gtttgactct tggtttcgac
     3481 gaaagtgagc ttgttcagaa tcgaatcgcc gtcg
//
LOCUS       DASSFQ010000216         8154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1130541, whole genome shotgun sequence.
ACCESSION   DASSFQ010000216 DASSFQ010000000
VERSION     DASSFQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130541"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3584)
                     /locus_tag="VFH96_07875"
     CDS             complement(<1..3584)
                     /locus_tag="VFH96_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6973928.1"
                     /translation="MSSVEPERLLHYRIIDKVGEGGMGQVYRAEDTKLGRNVALKLLA
                     PDATRDQTAKRRLLSEAQSASVLNHPNIVTIHAIEEVDGVDFIVMEFVEGETLTAHLA
                     NNGALPLPSLLDVGIQIADALETAHSIGLIHRDIKPANILITPKGVAKVTDFGLAKMV
                     RLSSDEIDREALTLAANLTGPGIVLGTAAYMSPEQTRGEPLDARSEIFSLGSLLYECA
                     TRTRAFNGPSVLAIMHAIAAIDPQPPSKLRPELPREFDIIIERALAKEKTLRYSSASE
                     MADALRSLRSTITGTWSGIPIVYDPDLIDRSSPSFVGRESEIQKLDALLQQAIKGTGR
                     MVFITGEPGIGKTSLSDEFLRRARKEQFGILISRGRCVEQYGTGEAYLPFLDAMGELL
                     QGTGRERIAAIMRTYAPTWCMELPTAFASSGSLEKLQQETIGATKERMMRELGDALSM
                     LASASPVVLLLEDLHWADPSSVDLLRHLSNRISTQRLLLAGTYRPEDVERSGHPLKNL
                     KTEMQVHNLCDEVTLTSWNRQHIAEYVDATFSPNDFPADLAALVHEKTEGHPLFVANL
                     LQYLGERGDVAKVNGCWSLAQPLAEMDLAAPESVRAMISRKIDALETEERRALQYASV
                     EGTEFLSSVTAKLLGVEEIDLEESLALISKSHRLIETLGEEELPDGTLATRYRFSHAL
                     YQNYLYGDLVNKRRVLLHQQAGEQLLSHYGKRAPQIATQLAIHFEQGRDFPRAVEYLT
                     HAGDHAAKLYGYAEAERHYTRALNLAQKLPEEEQPQHLSTLYHKRGTVNHAMGDFTQA
                     AKDFATMLDQTRVLDSLELQSTALNALTMSLFFSHRLDETVARATEALEVAERAGNAK
                     LRVETMFLIGLKHLCYGELREARGLLDEVVATSQKLNHKPGLLSGLTWRACLHFFQSE
                     YEPAVVAAKEALKLASELRDGFLMLCSMFFLGLSQGNQGKMSASLETLNEALKKAQRN
                     GDLFWHPRFPNCIGWIYRELYDFESARKYNEDGIEVARRHHVLEAEANSLINRGIDHT
                     RDGEHEQTAVAFHDVRDIFDRDAWFRWRYNIRLEAAMAEHWLRQNDLEKAREYVERLM
                     ATASEHGVHKYIAVAHNLMARISVRAGDLEAAEKHFDAALNELRDYPAPLVAWRVHAG
                     RARLKSRMGDVAGAEEASARATEIINLIASNVKDENLRNTFLSATTDK"
     gene            3791..5164
                     /locus_tag="VFH96_07880"
     CDS             3791..5164
                     /locus_tag="VFH96_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6973929.1"
                     /translation="MSDCQLGATQIKAKCGTYEVFENRATKKGRKISLKIVLISATGD
                     KREPEPLVYFAGGPGGSATEDAPGIAQAFPQILAHRDLLFVDQRGTGGSHPLDCKFYD
                     ASDLQTYLGYFFPLDDVRKCREQLETNSDLKLYLTTIAADDMDEVRAALGYERLNLFG
                     GSYGTRAALTYLKRHPQHVRAAVLQGISPTDHFMPSDFPMQTERALQGVLSECLADKV
                     CSEAFPNIKEETKAVLAQLIKGPVEVEIQKPKSNDRVKVQLSRDLAAEAIRYMLYSPV
                     PASRVPLILHLASQGNYVPLTYAAIQYRMNLVSGGSNGMYLSVTCAEDLPWIKPGEGE
                     RMAANTFLGDYRLRQQREACTLWPRAETERDYAQPVKSDVPVLILTGEWDPVTPPSNA
                     ERAAKTLTNSLHVVVPSGAHGLDGLEGMDCIDNLIVKFIEGGTTKGLDTSCVKNMHRK
                     GFALKFQ"
     gene            5161..5421
                     /locus_tag="VFH96_07885"
     CDS             5161..5421
                     /locus_tag="VFH96_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET6973930.1"
                     /translation="MRCQSCQTALPDDAQFCASCGLPTSRHPTDAATQLDQQQQTNAD
                     APRATDEQVGWLSSDGKKLTGTGKDAFSGSIGMKYEFSFTRK"
     gene            complement(5470..6651)
                     /locus_tag="VFH96_07890"
     CDS             complement(5470..6651)
                     /locus_tag="VFH96_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973931.1"
                     /translation="MKYLKQIGLSLAISTLVLGASDLANAQGRRGRGEERQQENQAAR
                     EQRQEERQSRRNEQQSQQQPQNSQERQQRWEQQRQQQQQNSQERQQRWEQQRQQQQQQ
                     RNEQQRHQQVQQSRERDDRWQQQRQEQIRQSQERDQRWQRDRQQQQQNRDWESQRQRQ
                     QQQQQLEQQRRQQSDRDRNRDWNDRNRDRNGRRDDNRNNNDNNWRQRNWRQEQEQRAS
                     RYSQDWQRRQSFLYQRQRLLEQQRRLNQLRYQQRYFERVRRDQLRLQQWRYNDYGPYS
                     YRYQRGGSYYETNRYGAEQLQRAINDGYGEGFQAGQADREDGYRFSPEEAYGYQDASY
                     GYDAYYVDLNEYQYYFREGFRRGYEDGFYSRSQYGRYSNGGYSILGTILEQILGLQLL
                     N"
     gene            6980..>8154
                     /locus_tag="VFH96_07895"
     CDS             6980..>8154
                     /locus_tag="VFH96_07895"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET6973932.1"
                     /translation="MKHEQTASAEAIPNAARIQRVEGEVALNNDVAGNTQTEDWIRAT
                     ENQPVSVGDRIYTRDNSRTSLAFTGRNFARLNPNTSLDVLALEDRRTQLALRNGSAVF
                     DVGYLNSGDLFEVATPYGAVDFQQPGLYNVGIDNGQVLISALSGLAQVVGLGGSGQIS
                     KGELLTLVGTTAANAVLSQLNGSDAGHLVDDYYSHQYPQYYDGRYRDYNAYLNDPYYF
                     DPYRRNVSYQYANSYIPGLYDLDYYGDWRNVDGYGYAWAPRVDNGWMPYQTGYWYTDY
                     PYGPTWVSSEPWGYAPYHYGRWAFVGDRWYWVPDRTNVDPVYSPALVAWVPFDQNQIG
                     WVPLGPGDVYVPHYYNNNTWDPYYLTRDNLHQRVVNLDVPGAVTVVSVDDFARDVDW"
BASE COUNT         1666 a         2327 c         2291 g         1870 t
ORIGIN      
        1 aacttgtcgg ttgtggcgct caggaaggta ttgcgaaggt tttcgtcctt aacgtttgag
       61 gcgatgaggt tgatgatttc ggtggcacgt gctgatgctt cttccgctcc cgcaacatca
      121 cccatcctgg acttcaatcg cgcccggccc gcatgaactc gccacgcaac cagcggcgcc
      181 gggtaatcac gcagctcatt caacgccgca tcgaaatgct tctccgcggc ttccaggtca
      241 cccgccctta cagaaattct cgccattaag ttgtgcgcaa ccgcgatgta cttatgtact
      301 ccgtgctcgc tcgcagtcgc catcaaccgt tcaacatact cgcgcgcctt ttcgagatcg
      361 ttctgacgca gccagtgctc ggccatcgcg gcttcgagcc ggatgttata acgccagcga
      421 aaccacgcat cccgatcgaa gatgtctcgc acatcgtgaa aagccaccgc cgtctgctca
      481 tgctctccgt cgcgcgtgtg atcgatacca cgattgatta gcgagttggc ctcggcttct
      541 agcacgtgat gccgccgcgc gacttcaatc ccgtcttcgt tgtacttgcg cgccgactcg
      601 aagtcgtaaa gctcgcgata aatccagccg atgcagttcg ggaaacgcgg atgccagaac
      661 agatcgccgt tgcgctgcgc cttcttcaat gcttcattca gcgtttcgag tgaagcagac
      721 atcttgccct gatttccctg cgataacccg aggaagaaca tcgaacacag catcaagaat
      781 ccgtcgcgca attctgaggc gagtttaagt gcctctttcg ccgccacgac tgcgggctcg
      841 tactctgact ggaagaagtg caaacacgcg cgccacgtca aaccactcag taacccgggt
      901 ttgtgattga gcttctgcga agtcgcgact acttcatcga gcaatcctct tgcttctctg
      961 agctcgccgt aacaaaggtg tttgagtccg atgagaaaca tcgtctcgac tcgcagtttc
     1021 gcgttaccgg ctcgttccgc aacttcaagc gcttcggttg cacgcgcgac tgtctcgtcg
     1081 agtcgatgtg agaagaagag cgacatcgtg agggcgttga gcgccgtcga ctgtaattcc
     1141 agcgagtcga gcacacgcgt ctggtccagc atggtcgcga aatctttggc ggcctgggtg
     1201 aagtcgccca tcgcatgatt caccgtcccg cgtttgtgat aaagcgtcga gaggtgttgc
     1261 ggctgctctt cttccggcag cttctgcgcg agattgagcg cgcgcgtgta atgccgttca
     1321 gcttccgcgt aaccgtaaag ctttgcggcg tgatcgccgg catgggttaa atattcgacc
     1381 gcgcgtggaa agtcccggcc ctgctcgaag tgaatggcga gctgagtcgc gatctgcggc
     1441 gcgcgcttgc catagtgact gagcaactgc tcgcccgcct gctggtgtaa caacacgcgt
     1501 cgtttgttta ccagatcgcc gtacagatag ttttgataaa gcgcgtgtga gaaacgatag
     1561 cgcgtcgcga gtgttccgtc cggcaactct tcttcgccga gcgtttcgat caaccggtga
     1621 ctcttgctga tgagtgccaa tgactcttcg agatcgatct cttcgacgcc gagcagtttc
     1681 gccgtcacac tcgaaagaaa ctccgtgccc tcgacactcg catactgaag cgcacgccgc
     1741 tcttcagttt cgagcgcatc gatcttcctg ctgatcatcg cgcgtacgct ttccggcgca
     1801 gcgagatcca tttccgcgag tggctgcgcc agcgaccagc aaccgttgac cttcgccaca
     1861 tcaccgcgct cgccgagata ctgcaaaagg ttggcgacga agagcgggtg tccttcagtc
     1921 ttctcgtgaa cgagtgcagc gagatctgcc ggaaaatcgt ttggcgagaa cgtcgcatcg
     1981 acatactcgg cgatgtgctg ccggttccac gatgtcaacg tcacctcgtc gcacaggtta
     2041 tggacctgca tctcggtttt aagattcttc agcgggtgac cgctgcgctc gacgtcttca
     2101 ggccggtagg ttccggcaag cagcagacgt tgcgtgctga tgcggttcga gagatgacgc
     2161 aggagatcga cgctcgacgg atcggcccag tgcaagtctt ccaacaacag caccacaggc
     2221 gacgcagacg cgagcatgct cagcgcatcg ccgagctcgc gcatcatgcg ttccttcgtc
     2281 gcaccgatgg tttcctgttg cagcttctcc agcgatccac tcgacgcaaa cgcagtcggc
     2341 aattccatgc accacgtcgg cgcgtaagtg cgcatgatcg cggcgatgcg ttcgcggcca
     2401 gttccctgaa gcagctcgcc catcgcgtcg agaaacggca aataggcttc gcccgtgccg
     2461 tactgttcga cgcaacgacc acgcgagatc agaatcccga actgctcttt tctggcgcga
     2521 cgcaggaatt cgtcggacag cgaagtctta ccgatgccgg gttcccccgt tataaacacc
     2581 atgcgacctg tgcctttgat cgcttgctgt aacagcgcgt cgagtttttg gatctctgat
     2641 tcgcggccta caaacgaagg cgacgagcga tcgatcaaat ccggatcgta aacaatcggg
     2701 atgccggacc acgttccggt gattgtcgat ctgagactgc gcaacgcatc cgccatctca
     2761 cttgctgatg aatagcggag cgttttttct ttcgcgagtg cgcgctcgat gattatgtcg
     2821 aactcacgcg gcagctcggg ccggagtttg ctcggcggct gcggatcgat cgcggcaatc
     2881 gcatgcatga tcgcgagcac gctcggacca ttgaacgcgc gtgttcgcgt cgcgcattcg
     2941 taaagcaggc tgccgagcga aaagatctct gaacgggcgt cgagcggctc accgcgagtc
     3001 tgttcgggag acatgtaggc agcggtgccg agcacgatgc cgggtccggt caggtttgct
     3061 gcgagcgtga gtgcttcgcg atcgatctcg tcagagctga gacggaccat cttcgcgagt
     3121 ccgaagtccg tgactttcgc gacgcctttc ggagtgatca gaatattggc tggtttgatg
     3181 tcgcgatgta tgagtccgat cgagtgcgca gtctcgagtg cgtcggctat ttgaatgccg
     3241 acatccagca gtgacggcaa aggcagggcg ccgttgttgg cgagatgcgc agtcagtgtt
     3301 tcgccctcga caaactccat gacgatgaaa tccaccccgt cgacttcttc gatcgcgtga
     3361 atcgtgacga tgtttggatg attcaggact gaggccgact gcgcttccga gagcaggcgg
     3421 cgcttggcgg tttgatcgcg cgtggcgtca ggtgcgagca gcttgagggc gacgttgcga
     3481 ccgagcttcg tgtcttccgc acgatacacc tggcccattc cgccttcacc tactttgtcg
     3541 atgatgcggt agtgcagcag ccgttctggt tcgacagaac tcatgggggc tctggggatc
     3601 atagaggtta cacatagaac gcgtaaagcc ggccacgaaa aggcacaaaa ggcacaaaaa
     3661 atttcttgta aatcctgcaa atcctgtcat tctgtcaaaa actatgcgta ctacaatgct
     3721 tatcgccatc gcactattac tcacccttca atccgcgagc gcggtcctac aaaatggatc
     3781 caaagtggtt ttgagtgact gccagctcgg cgctactcag atcaaagcaa agtgcggcac
     3841 ctatgaagtc ttcgaaaacc gcgccactaa aaagggccgg aagatctctc tgaagatcgt
     3901 cctcatctcc gccactggcg acaagcgcga accggagcct ctcgtttact tcgctggtgg
     3961 accaggcggc tcagccaccg aagacgcccc cgggatcgcg caggcatttc cacagatact
     4021 cgcgcatcgc gacctgctct tcgtcgatca gcgcggcact ggcggctcgc atccactcga
     4081 ttgcaagttt tacgacgcgt ccgatctgca aacgtatctc ggttatttct tcccgctcga
     4141 cgatgttcgc aagtgccgcg aacaactcga aaccaattcg gatctgaagc tctacctaac
     4201 cacgatcgcc gccgatgata tggacgaagt acgtgcggcg cttggctacg agcgactcaa
     4261 tctcttcggc ggctcttacg gcacgcgagc ggcgctgact tacctgaagc gacatccgca
     4321 gcacgtgcgc gccgcggtgc tccagggcat ctcaccgacg gaccacttca tgcccagcga
     4381 ttttccaatg cagacggagc gagcgcttca gggagttctc tcggaatgtc tcgccgacaa
     4441 ggtttgcagt gaagcctttc cgaatatcaa agaggaaacg aaagcagttc tcgcacagtt
     4501 gatcaaaggt ccggttgaag ttgagattca aaaaccaaaa tcaaacgatc gtgtgaaagt
     4561 acaactttct cgcgatctcg cggcggaagc gattcgctac atgctttatt caccggtgcc
     4621 ggccagtcgc gttccactga ttctgcacct cgcgtcgcag ggcaattacg tgccgttgac
     4681 ctacgccgca attcagtacc ggatgaatct cgtgtcgggc ggcagcaacg gcatgtatct
     4741 ctcagtgact tgtgccgaag acttgccatg gataaaaccc ggcgagggtg agcgaatggc
     4801 ggcgaacacg tttctcggtg attaccgtct gcgccagcaa cgcgaagcct gcacgctctg
     4861 gccacgcgcg gagaccgagc gcgattacgc gcagccagtt aaatcagacg taccggtttt
     4921 gattttgact ggcgaatggg atccggtgac gccgccgtcg aatgctgaac gtgcagcgaa
     4981 gacgttgacg aacagtcttc acgtcgtcgt gccgagtggc gcgcacggtc ttgacgggct
     5041 cgaaggcatg gactgcatcg ataacttgat cgtgaagttc atcgaaggcg gaacaaccaa
     5101 aggtctcgac acgtcgtgcg tcaagaatat gcatcgcaag ggatttgcac tgaaattcca
     5161 atgaggtgcc agagctgtca gaccgcactg cccgacgacg cgcaattctg tgcgagttgc
     5221 ggtctgccga catcacgcca tccgaccgac gccgccacgc agctcgacca gcaacaacaa
     5281 acaaacgctg atgctccgcg agcgacagac gagcaagtcg gatggctttc gagtgatggt
     5341 aagaaactca cgggaacggg caaggatgcg tttagcgggt caatcgggat gaagtatgag
     5401 tttagtttta caaggaaata ggtcctatag gacatatagg acatatagga cctattcttg
     5461 taataacaac tagttcaaaa gctgcagccc gaggatctgt tcgaggatcg taccgagaat
     5521 gctgtagcct ccattcgagt agcggccgta ttgtgagcgg ctgtagaaac cgtcctcata
     5581 cccgcgcctg aaaccttcac ggaagtaata ctgatactcg ttcagatcga cgtaataggc
     5641 gtcgtagccg taactcgcat cctggtagcc ataagcctct tcaggactga agcgataacc
     5701 atcctcgcga tcggcctgcc cggcttggaa gccttctccg taaccatcgt taatcgcgcg
     5761 ctgcaactgc tctgcgccgt aacgattggt ctcgtagtag ctgccgcccc tctgatagcg
     5821 atagctgtac ggaccgtagt cgttataacg ccactgctgc aatcgcaact gatcgcgacg
     5881 cactcgttcg aaataacgct gctggtaacg caactggttc aaacgtcttt gctgttcgag
     5941 cagacgctga cgctggtaca ggaagctctg gcgtcgctgc cagtcttgac tgtagcgtga
     6001 cgcacgctgt tcctgttcct gtcgccagtt gcgctggcgc cagttgttgt cgttgttgtt
     6061 gcgattgtca tcgcgacggc cgttccggtc acgatttcga tcgttccaat ctcgattacg
     6121 atcgcgatct gactgctggc gacgttgttg ttcgagttgc tgttgctgct gttggcgctg
     6181 tcgttgtgat tcccaatcgc gattctgctg ttgttgctgg cggtctcgct gccaacgctg
     6241 atcacgttcc tgcgactggc ggatctgttc ctgtcgttgc tgctgccagc gatcgtcgcg
     6301 ttcgcgggat tgctggacct gctggtgtcg ctgttgctcg ttacgttgct gttgctgctg
     6361 ctggcgctgc tgttcccaac gctgctgccg ttcctggctg ttctgctgct gctgttggcg
     6421 ttgctgctcc cagcgctgtt gtcgttcctg gctgttctgc ggttgctgtt gtgattgttg
     6481 ttcgttacga cggctctgtc gttcttcctg tcgttgttcg cgtgcagcct ggttttcctg
     6541 ctgtcgctcc tcaccccgac cccgtcgtcc ctgggcattg gcgaggtcgc ttgctcccaa
     6601 cacgagagtg cttatcgcca gagatagacc gatctgtttt aaatatttca cctttgattt
     6661 cctttcagtc cgacgggaga gaggtcgact ccggcgtatt tggcaagcgt cattccacac
     6721 gagaataagg ccggattccg gtgcatctat gtaggtttac gagacgatat caagcaggaa
     6781 gtaacgatat gggatagtaa tttgggccac ggatcacacg gatcaggtac ggatcccgca
     6841 ttggcatgga ggttgcggaa tccccctgaa aaatagcttt gaggggggct tttaaatgag
     6901 acaaataaaa gtgtggccac acctcacgat cgtggcggta gtgtgtgccg tcctggccgg
     6961 agctggcgtg gccctgtgga tgaagcacga acagaccgcg tcggctgagg cgataccaaa
     7021 tgcagcgcgt attcagcgcg ttgaaggtga ggtggcgctc aacaacgacg tggccggaaa
     7081 cacccaaacc gaagattgga ttcgcgccac tgagaaccaa ccggtctcgg tcggcgatcg
     7141 catttacacg cgcgacaatt cgcgcacgag tttggcattt accgggcgca acttcgcgcg
     7201 gctcaatcca aacacgtcgc tcgatgtgct tgcgctcgaa gaccgtcgca cgcagctcgc
     7261 tctgcgtaac ggatcagccg tgtttgacgt cggctatctg aactcgggag atctattcga
     7321 agtagcgacg ccgtatggtg cagtcgattt tcagcaacct ggtctctaca acgtcggcat
     7381 cgacaatggt caggtgttga tctcagcgct gagtggtctc gcgcaagtcg tcggactcgg
     7441 cggcagtggc cagatcagca aaggtgagtt gttgacgctt gtcggcacga ccgccgcgaa
     7501 cgcggttctt tctcagctta atggaagcga tgcgggtcac ctggtggacg attactattc
     7561 ccaccagtat ccgcagtatt acgacggccg ctatcgcgac tacaacgctt atcttaacga
     7621 tccttattac ttcgatcctt atcggcgcaa tgtcagttac cagtatgcga actcatacat
     7681 tcccggcctc tatgatctcg attactacgg cgattggcgc aatgtcgacg gctacggcta
     7741 tgcctgggcg ccgcgcgtcg ataacggctg gatgccctat caaacgggtt actggtacac
     7801 ggattacccg tatggtccaa cgtgggtttc gagcgagccg tggggctacg cgccttatca
     7861 ctacgggcgt tgggcctttg tcggggatcg ctggtactgg gtgccggatc gcacgaatgt
     7921 cgacccggtc tactcgcccg cactcgtcgc gtgggttccg ttcgaccaaa accagatcgg
     7981 ctgggtgccg cttggcccag gtgacgtcta cgtgccgcac tattacaaca acaacacgtg
     8041 ggatccttat tacctgacac gtgacaatct ccaccagcgc gtggtgaatc tcgacgtgcc
     8101 tggagccgtt actgtcgtga gtgtggacga cttcgcgcgt gatgtcgatt ggag
//
LOCUS       DASSFQ010000217         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2186445, whole genome shotgun sequence.
ACCESSION   DASSFQ010000217 DASSFQ010000000
VERSION     DASSFQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186445"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFH96_07900"
     CDS             <1..445
                     /locus_tag="VFH96_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973933.1"
                     /translation="NDIEKATELARAMVCEYGMSELGPLTFGKKEEQIFLGREIAQHR
                     DFSEDTAIKIDQEVKSIVTSQYERAKAIIEDNRDTMIRLAECLLERESLDGVEIRRIV
                     AGLPLDENPSIDSGDGEDRPQLKEPTAKPLKPILPPITGGNPAPA"
     gene            528..1370
                     /gene="folP"
                     /locus_tag="VFH96_07905"
     CDS             528..1370
                     /gene="folP"
                     /locus_tag="VFH96_07905"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET6973934.1"
                     /translation="MPIGERTLIMGILNVTPDSFSDGGQFVNLVSALAHAEQMIAEGG
                     DIIDVGGESTRPGGEPVSVEEEISRVVPVIEALAQRTDTPISVDTTKSEVARAALDAG
                     AAIVNDISALRFDFYVADAVARAGAGLVLMHSRGTPATMHRLPPVADIMHEVTHSLRA
                     SINMAERRGVKRESIVIDPGIGFGKSQEQNLELLAKLDRLIAAFPDYPLLIGTSRKSF
                     IGRLLADESGTPAAAEDRLYGSLATITAAILKGAHIVRVHDVKATAETIRVAESIREN
                     PGQM"
     gene            1386..1934
                     /locus_tag="VFH96_07910"
     CDS             1386..1934
                     /locus_tag="VFH96_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HET6973935.1"
                     /translation="MTKLLRVSLFVVMLCFVSGFAECYKPVTKNQLPARIKTIAVPAF
                     QMESQALRYKIESRFADAVMRELVHRGRGLRVQGEREGADAVIEGVIKSFNFGGVLLD
                     DKGRARIYEVTIVGAVTVRDQTENRVLYDNQNFVFRGEYEFGSDPRSFFNEEDPAVLR
                     ISRNFAESIVSTLINAVQLEKK"
     gene            1931..2929
                     /gene="holA"
                     /locus_tag="VFH96_07915"
     CDS             1931..2929
                     /gene="holA"
                     /locus_tag="VFH96_07915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET6973936.1"
                     /translation="MKALSRTELDRSLREGLRPLYLLLGSEVYLRRVAAQTITEAALS
                     RTLLREFNESAFSLVSDPVQSAIAAADQLPMMSDMRVVRIRDFAKLREADEDVLIRYL
                     NNPAPTTVMIFIADELDKRKKSSKVLLDTCTVVDFPPLKDAEAKAWAKTRLKELKVSA
                     DDAVLSDLIRLVGTDVQTLFNELEKLASATASTGNRITLDSIEELIGRSRELSNFELG
                     DHLMAGNRKKALETLNRLLEDGAEPVMLVGLIAGNYHRLALGKHLLTRGGREEVFRNI
                     NLPPFKRDSYISMLQRSTATKIARGIQLTAAADLAIKTSQATPRLQLELLVCELAT"
BASE COUNT          704 a          906 c          811 g          612 t
ORIGIN      
        1 caacgacatt gaaaaggcga ccgagctggc gcgggcgatg gtctgcgagt acggcatgtc
       61 cgagctgggg ccgctgacct tcggcaaaaa ggaagagcaa atcttcctgg gccgtgaaat
      121 cgcacagcat cgtgatttct ctgaagacac cgcgatcaag atcgatcaag aggtgaagag
      181 catcgtcact agtcagtatg aaagagcaaa ggcgatcatt gaagacaatc gcgacacgat
      241 gattcgcttg gctgaatgtc tgttggagcg tgagtcgctg gatggagtcg agattcgcag
      301 gattgtcgcg ggcttgccgc tcgacgagaa tccgtcgatc gactcgggtg atggcgaaga
      361 tcgtccgcag ctcaaggaac caactgctaa accgctgaag ccgatactgc caccgataac
      421 tggcggcaat ccggctcccg cctaaaatcc tatccacaga ttacgcagat tttgtaatct
      481 gtgtaatctg tggataaatg acttcctgga aaatacgaga tcgagaaatg cccattggtg
      541 agcgaacgct catcatgggc attttgaatg ttacgcctga ttccttcagc gacggcggac
      601 agtttgttaa cctcgttagc gctttggccc acgccgaaca gatgattgcg gaaggcgggg
      661 acatcatcga cgtcggcggc gagtctaccc ggcccggcgg cgaaccagtt tccgtcgaag
      721 aagaaatcag ccgcgtcgtt cccgtcatcg aagccctggc gcaacgtacc gacacaccaa
      781 tctcagtcga caccaccaaa tccgaagtcg cacgcgccgc actcgacgcc ggcgccgcaa
      841 tcgtcaacga catctcagcg cttcgtttcg acttctacgt cgcagatgca gtcgcccgcg
      901 ccggcgccgg actcgtcctg atgcactccc gcggcacacc cgcgacgatg catcgtcttc
      961 cacctgtcgc cgacatcatg cacgaagtca cccacagcct gcgcgcgagt atcaacatgg
     1021 ccgagcgacg cggcgtcaag cgcgaatcga tcgtgatcga tcccggcatc gggttcggaa
     1081 aaagccagga acaaaacctc gaacttctcg ccaaactcga tcggctaatc gccgcgtttc
     1141 ccgactaccc actcctgatc ggcacctccc gcaaatcctt catcggccgc ctccttgccg
     1201 acgaatccgg caccccagcc gccgctgaag accgcctcta cggctctcta gccaccatca
     1261 ccgccgcaat tttgaaaggc gcacacatcg tccgcgtcca cgacgtgaaa gcaaccgccg
     1321 aaacaatccg tgttgcggag agcatccgtg aaaatccggg gcaaatgtga gagaatccac
     1381 cgcgaatgac aaagctcctg cgtgtttccc tcttcgtcgt gatgctctgc ttcgtttccg
     1441 gttttgccga gtgctataag ccggttacca agaatcaact tccagctcgt atcaaaacaa
     1501 tcgcggtgcc ggcatttcag atggagagtc aggcgctacg ctacaagatt gagtcgcgtt
     1561 tcgcggacgc ggtgatgcga gaactcgtcc accgcggccg tgggctgcgc gtgcagggag
     1621 aacgcgaagg cgccgacgcc gtgatcgagg gcgtcatcaa gagtttcaat ttcggcggcg
     1681 tgctgctcga cgacaaagga cgcgcgcgaa tttatgaggt tacgatcgtt ggcgctgtaa
     1741 ccgtgcgcga tcaaaccgag aatcgcgtgc tttacgataa tcagaacttc gtcttccgcg
     1801 gcgagtatga attcgggagc gacccgcgca gtttcttcaa cgaagaggat ccggcagtgc
     1861 tccgcatctc gcgtaacttc gccgaaagca tcgtctccac actcatcaac gccgtccaac
     1921 tcgaaaagaa atgaaagcgc tgtctcgaac cgagctcgat cgctcgctgc gtgagggttt
     1981 gcgcccgctt tatctgctgc ttggctcaga agtctattta cgtcgagttg cagcgcaaac
     2041 gatcactgaa gccgcgctca gccgcacgct tttgcgcgag ttcaacgagt cggcatttag
     2101 tttggtgagc gatccggtgc agtctgcgat cgcggcggcg gaccaactgc caatgatgtc
     2161 ggacatgcgc gtggttcgga ttcgcgattt cgcgaagttg cgcgaggcag atgaggatgt
     2221 gctgattcga tacctgaaca atccggcgcc cacgacggtg atgatcttca tcgccgacga
     2281 gctcgacaaa cgaaagaagt cgagcaaggt gttgctggac acgtgcaccg tcgtcgactt
     2341 cccgccactc aaagatgcgg aagcaaaagc gtgggccaag acacgtttga aggaattgaa
     2401 agtttctgct gatgatgcgg tattgagcga cttgatccgg ttggttggga ctgacgtgca
     2461 aactctcttc aacgaactcg agaaactcgc ttcagccacg gcgagcacag gcaatcgcat
     2521 cacgctcgat tcgatcgagg aactgatcgg acgatcgcgt gagctatcaa atttcgaact
     2581 cggagatcac ctgatggcgg gcaatcgcaa gaaggcactc gagaccctga atcgtcttct
     2641 cgaggacggc gccgagccgg tgatgctggt tgggctgatc gccggcaatt atcatcgtct
     2701 cgctctcggt aaacacctgc tcacacgcgg cggtcgggaa gaagtctttc gcaacatcaa
     2761 tctgccgcct ttcaaacgcg actcttacat ttccatgctg caacgcagca cggcaaccaa
     2821 aatcgcccgt ggaatccaac tcaccgccgc cgccgatctc gccatcaaaa cctctcaagc
     2881 cactccccgt ctccaactcg agctacttgt ttgcgaactg gcaacctaat ccgcgttcat
     2941 ccgcgttcat ccgcggctaa aactactctg cacttctcaa cgattgattc atccccaact
     3001 tggtccaacc gctccgcaat acgctcaatc caa
//
LOCUS       DASSFQ010000218         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1294792, whole genome shotgun sequence.
ACCESSION   DASSFQ010000218 DASSFQ010000000
VERSION     DASSFQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294792"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1378)
                     /locus_tag="VFH96_07920"
     CDS             complement(71..1378)
                     /locus_tag="VFH96_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6973937.1"
                     /translation="MNRPSRNSFKAPLLVLAVLCLFVSPLALPTSRAARNDDSSLVST
                     ATPEGRLAVFDDLWETIEDHYYDPGFNGVDWQAKRALFRPAAAKASSTHEFYELLRQM
                     IASLRDAHTRVYAPDEKFDWWNPRFVTVGLAVREVQGVPVVIQVDPSSMAARSDVRAG
                     DVIVSVNELPVKEFIKQRLESLGTSDDERARYRAIAGLFDGPAGSTVRVAWTTREGKH
                     KSAVLQRYWTQRQLGFTTQRKGNLAILRIDAFTQSLALDFSKALATALDGAEGIVLDL
                     RSNGGGDAEAMADVASLFLDDGINLGKFADRSGASFELQTYSKRLWRVPQLSQIKLPL
                     VVLTSESTSSAAEIMAAALQAKRRARVIGTGTCGCVLAIRNRHALPDGGLLDVSEFDY
                     RTAGGVRLQDRGVTPDESTRVTRSDIYSRRDPALEAARRFLEK"
     gene            complement(1416..2294)
                     /locus_tag="VFH96_07925"
     CDS             complement(1416..2294)
                     /locus_tag="VFH96_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6973938.1"
                     /translation="MIRLAFLICGLLVVAGNAYARTSISVLDFGSTDFGKLVAEKLHA
                     QLKAEPEFLVADADLSRSAANGVGYAGSLNLTLTEARDLGSALATDYYLLGDAQLLRR
                     SSFEKPVYYESYCSVFLVSARTGRLLLWERPSFANLYSVQARDLLLKEITNGELSRRL
                     LIATRRANEDERIQRAVISTSSAPVIEEAPDDEKAAEAQGLRLPRPFRRLRPEYPDTA
                     ARAEAEATVDVLVDVGADGEVGEVQVVRWAGFGLDEATIATIKQLHFFPALKNGGAIP
                     MRVLLRYNFRKPPPRQ"
     gene            complement(2291..3601)
                     /locus_tag="VFH96_07930"
     CDS             complement(2291..3601)
                     /locus_tag="VFH96_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6973939.1"
                     /translation="MTERIYRDPVHNIIRLQTDSDEGELMMRLIDASEFQRLRRIKQL
                     GLGLYTYQGAEHSRFTHSLGAFHLMTRVLNRFSERYSIAPADRVAARAAALLHDVGHG
                     SFSHVMEKLINFHHEQRTVQVILEEGSEIGALLKSYSSDLPRNVASIIEGSFKPSALA
                     QLVSSQLDVDRMDYLLRDSLMTGAKYGIYDLEWIINALAIDEDADRIYVEARGLYAVE
                     EYLQARYYMFRQVYFHRTLRSAEAALRSIIRRALRMLDEGQEVWHAPATAFEKILRRE
                     ELSVTEHLQIDDSDFVFHVKQWQSSSDAILSDLSRRFTGRRLFKAIDLDMPQEQQAEF
                     LRGAKKIVERAGFDPEYYFVEDRASDLPYYNYYAAEKAEPKTHIYVESGYASPQIREI
                     SEVSNVVRGLQQPYELHRICFPPEVKTEVYELYHRGAVHKRTVG"
BASE COUNT          813 a         1082 c         1010 g          757 t
ORIGIN      
        1 aagtagagca ctcgccaatc ttacgcttaa agcggtgctt acgagtagag gcgattttgc
       61 aaccgggatt ctacttctcg agaaaacgcc tggctgcttc gagtgcgggg tcccggcggg
      121 agtagatgtc tgagcgcgtg acgcgggttg attcgtcggg agtgacgccg cggtcctgga
      181 gacggacacc gcccgcggtg cggtagtcaa attcactcac gtctaataag ccgccgtcgg
      241 gcaaggcgtg tctgtttctg atcgccaaca cacaaccaca cgttccggtg ccgatcacac
      301 gcgcgcggcg tttcgcctgc aacgccgcag ccatgatctc cgcggcgctg gacgtgctct
      361 cgctggtcaa gaccacgagt ggcaacttga tctgcgacaa ctgcggcacg cgccacaaac
      421 gcttggagta agtttgcaat tcaaacgacg cacccgaacg gtccgcaaac ttcccgaggt
      481 tgatcccgtc gtcgagaaaa agcgaagcga cgtcagccat cgcctcagcg tcgccgccgc
      541 cgttcgaacg cagatcgagc acgatgcctt ccgcgccgtc caaggcagtt gccagtgctt
      601 tggaaaaatc tagggcgaga ctttgcgtga acgcgtcgat cctcaggatc gcgaggttgc
      661 ctttcctttg ggtcgtaaac ccgagctggc gctgagtcca gtagcgctgc aacaccgcgg
      721 atttatgttt tccttcgcgc gtggtccatg cgactctgac tgtgctaccc gctggcccat
      781 caaacagacc ggcaatcgct ctatagcgcg cgcgttcgtc atcactcgta cccaaactct
      841 cgagccgttg cttgatgaat tccttcaccg gcagttcgtt gacgctcaca atcacatcgc
      901 cggcgcgaac gtcactgcgc gcggccatgg agctcgggtc aacctgaatc acgaccggta
      961 cgccttgaac ttcgcgcacc gctaaaccga cggtcacaaa acgcgggttc caccagtcaa
     1021 acttttcatc cggcgcatag acacgcgtgt gtgcgtctcg cagcgacgcg atcatctgtc
     1081 gcagcagttc gtaaaactcg tgcgtgctgc tcgcctttgc ggctgcgggt cgaaatagag
     1141 cgcgcttagc ttgccagtcg acgccgttga atcccggatc gtagtagtgg tcttcgatcg
     1201 tctcccaaag atcatcgaat accgcgaggc gtccttcagg cgtcgcggtc gagacgagtg
     1261 acgaatcgtc gttccgcgcg gcgcgcgagg ttggcaacgc gagcggcgaa acgaaaagac
     1321 agagaactgc aaggactagc aggggagcct taaatgagtt tcgcgaggga cgattcattc
     1381 acttcctcgg gggcattcac acctcgggac caaaatcact gccgtggagg cggcttgcgg
     1441 aaattgtaac gcaacaacac ccgcatcggg atcgcgccgc catttttcag cgccggaaaa
     1501 aagtgcaact gcttgattgt ggcgattgtg gcttcatcca gcccgaaacc agcccagcga
     1561 acaacctgca cttccccgac ttctccatcc gcacctacgt caaccagaac atcaaccgtt
     1621 gcttcagctt ccgcgcgcgc cgcggtgtcg ggatattcgg gccgcaaccg tcgaaacgga
     1681 cgcggcaaac gcaaaccctg cgcttcggcc gccttttcat cgtcgggagc ttcttcgatc
     1741 accggtgcgg aagaggtcga gataaccgca cgttggatac gctcatcttc attcgcacgt
     1801 cttgtggcga tcagcaagcg gcgcgacaat tcgccgttgg taatctcttt caacagcaag
     1861 tccctggctt gcacgctgta aaggttcgca aaactcgggc gctcccacaa cagcaaccgg
     1921 cccgtgcgcg cgctgaccag aaacaccgaa caatacgact cataataaac aggcttttca
     1981 aacgacgaac ggcgcaacag ttgcgcgtcg cccagcaggt aatagtcggt tgcgagggcc
     2041 gagcccagat cacgcgcctc agtcagcgtt agattgagcg atcccgcata accaacaccg
     2101 ttggctgccg agcgagacag gtcagcatcg gcaaccaaaa attccggctc ggctttcaac
     2161 tgcgcgtgaa gcttttcggc aacgagcttt ccaaagtcgg tggaaccaaa gtccaataca
     2221 ctgatgctcg tgcgcgcgta agcgttcccc gcgaccacga gcaatccaca aatgagaaaa
     2281 gcgagacgga tcatccgaca gttcgtttat gcaccgcgcc gcgatgataa agctcgtaga
     2341 cttcggtctt cacttcgggc gggaaacaga tgcgatgcag ctcatacggt tgctgcagtc
     2401 ctcgcaccac attactgact tcactgatct ctctgatctg cggcgacgca taaccacttt
     2461 ccacgtagat gtgcgttttg ggctccgcct tctcagcggc gtagtagttg tagtacggca
     2521 gatcgctcgc gcgatcttcg acgaagtaat actccggatc gaaaccagcc cgctcgacga
     2581 tctttttcgc cccacgaaga aactccgcct gctgttcctg cggcatgtcg agatcgatcg
     2641 ctttgaagag acgccggccg gtaaagcgcc ggctgagatc actcaggatc gcatcactcg
     2701 agctctgcca ttgtttgacg tggaagacga aatcagaatc gtcgatctga agatgttcgg
     2761 tgaccgacaa ctcttcacga cgaagaatct tttcgaacgc ggtggcgggc gcgtgccaaa
     2821 cctcctggcc ttcgtcgagc attcgcagtg cgcgtcgaat gatcgatcgc aacgcggctt
     2881 ccgctgatcg caacgtgcga tgaaagtaga cctggcgaaa catgtagtaa cgcgcctgca
     2941 agtattcttc aactgcataa aggccgcgcg cttcgacgta aatccgatcc gcatcttcat
     3001 cgatcgccag cgcgttgatg atccactcga gatcgtagat accgtacttc gctccggtca
     3061 tcagcgaatc gcgcaacagg tagtccatgc gatcgacgtc gagctgcgac gacacgagtt
     3121 gggccaaagc ggatggtttg aatgaaccct cgattattga ggcaacgttg cgcggcagat
     3181 cggaagaata ggatttcagc agtgcgccga tttcggatcc ttcttcgagt atcacttgca
     3241 ccgtgcgctg ctcgtgatga aagttgatca gcttctccat cacgtgcgag aacgatccat
     3301 ggcccacgtc gtgcagcaac gcggctgcgc gcgccgcgac gcgatcggcc ggagcgattg
     3361 aatatctttc gctgaagcga ttgagcacac gcgtcatgag atgaaacgcg ccgagtgagt
     3421 gagtgaagcg tgagtgttcc gcgccctgat acgtgtagag accgagtccg agttgtttga
     3481 tccggcgcaa acgttgaaac tccgacgcat cgatcagacg catcatcaac tcgccttcgt
     3541 ctgagtcagt ttgcagccgg attatgttgt ggacggggtc acgatagatg cgttctgtca
     3601 ttcggcccta aatatataac agcggtctcc ggaatccagc ggaaacggtt gaaatttctg
     3661 ga
//
LOCUS       DASSFQ010000219         6846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_996631, whole genome shotgun sequence.
ACCESSION   DASSFQ010000219 DASSFQ010000000
VERSION     DASSFQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996631"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFH96_07935"
     CDS             complement(<1..364)
                     /locus_tag="VFH96_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET6973940.1"
                     /translation="MSTAVKQYHEEEAIGKTYDLQVARRLLRYLKPYRRLLLGALALT
                     LVLNLLGVLQPKFTQYAIDWYILPRQTNGLSLLVGLYLASQLLRLIFSYFQSIFVNSI
                     GQYVMFDLRKELYSKLQHL"
     gene            complement(431..2173)
                     /locus_tag="VFH96_07940"
     CDS             complement(431..2173)
                     /locus_tag="VFH96_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6973941.1"
                     /translation="MEDLKKFALYFRNYKLQLTLGILCILGSVTVGLLIPRIVGKAID
                     ANWTEISWWKLTISAAKVLGASLISGLFLYLQRRILIGMSRNIEYDMRDEFYRHLVNQ
                     PLSFFHEHRTGDLMARATNDMSAVRQLAGPMIMYSLSTVFVVIFVLPVMFMLNWWLTL
                     LLFVTMPLVSLTVKFFGAQVHTRFEKIQDFFGQITARAQENFSGVRVVRAYAQEGAEM
                     AAFNNLNRQYAERNLGLVKIDALMRPLMTFLIGFGFVLIIWAGVPLAVRGEITVGEFT
                     MFNLYLTRLIWPLIALGYVVNLYQRGTASLKRLNAILRIEPEIADLPGTVTQPPIKGK
                     IEFRDLTFSYHPNDEPVLKNVTLTIPEGQTVAFVGRTGSGKSTVANLIPRLIDAPENK
                     LFIDEVDIHDYPLAQLRESIGYVPQETFLFSDSVKGNIAFGVADAKMAEVEWAAEIAG
                     LSDDIAGFPDRFDAMVGERGITLSGGQKQRTAIARAVLREPKILILDDALSSVDTYTE
                     EKILGQLRGVMRDRTSVIISHRVSTVREADMICVLDDGRIIEQGTHDELLALGGEYAD
                     LHERQLLEEELAAT"
     gene            complement(2289..2663)
                     /locus_tag="VFH96_07945"
     CDS             complement(2289..2663)
                     /locus_tag="VFH96_07945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6973942.1"
                     /translation="MTLDHVPFAKLLGIEVDSAGPGHAVLSMKLHDDLMRNNGIAHGG
                     AIATLIDSAMAIAIMAQLAEDERTVTVDLTIHYLRPISEGTARASARVVRAGRRVITV
                     SAELFDDAGKLSATAISTYLRM"
     gene            complement(2660..3073)
                     /locus_tag="VFH96_07950"
     CDS             complement(2660..3073)
                     /locus_tag="VFH96_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HET6973943.1"
                     /translation="MSSDSMQPRVPYAVSPPRYSVSHQMVTTAFELPNYRVVQGLGVV
                     RGIVVRSRNIFATIGASLQTIVGGNITVWTTLCEETRAHAFDIMIQHATEIGANAIVG
                     ARYESTELAQGVTEVLAYGTAVIVEPANPEQSYRS"
     gene            complement(3070..3402)
                     /locus_tag="VFH96_07955"
     CDS             complement(3070..3402)
                     /locus_tag="VFH96_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET6973944.1"
                     /translation="MDKENPKYRERVYKIVNRIPSGRVMTYGQIAELLGEGYTPRTVG
                     FCMHSSPEGTPWHRVINAQGKCSTGRVVLPHNKQQLMLEYEGVVFDKNGRCDLQQFLH
                     IPRQGRKR"
     gene            complement(3430..4074)
                     /locus_tag="VFH96_07960"
     CDS             complement(3430..4074)
                     /locus_tag="VFH96_07960"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6973945.1"
                     /translation="MPIFDVTVPVSNELPTWPTDPAVEISDFCSLAAGDGANVSMLNF
                     GAHTGTHVDAPAHFIEGATKVESLSLDALIGEATVIEVPEEATAIDEEFVRKNYVAGT
                     ERVIFKTRNSAFWNEAKPQFHTDFTYLDLPAARWLVEQGIKLVGIDYLSIEKYGSGKH
                     ETHLALLSRGVVILEGLNLTGIAAGKYELICLPLRLRSNKGDGAPARVVLRTSV"
     gene            4149..4946
                     /locus_tag="VFH96_07965"
     CDS             4149..4946
                     /locus_tag="VFH96_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HET6973946.1"
                     /translation="MNSPFRLAASSYLNSAPLIWSFLHGPHRGNVDFVEAVPARCAQL
                     LRDSSVEGALVPVIEYQRIEGGSLVQNVCVGSQKEVLSVVLVSKNKRLEDIRTVALDE
                     SSRTSATLVKIIFREFLDHEPEWTTRAPQLEEMLEKNDAALIIGDPGMAFPRHGLNVW
                     DMAALWKQHTKLGFVFAMWMVRDDAGERARAVDFSGARDEGVAQIEEIVRSYQDKIPM
                     RVDELRNYLTENIVFKVDESMEKGLRLYFELAFKHGLIERVKPLRFI"
     gene            4946..6043
                     /gene="mqnC"
                     /locus_tag="VFH96_07970"
     CDS             4946..6043
                     /gene="mqnC"
                     /locus_tag="VFH96_07970"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HET6973947.1"
                     /translation="MSATNIQPILDRVLAGERMTVDECTTLLESHDLARMGVAADEIR
                     ARRHPDNIVTYIIDRNINYTNVCNVVCTFCAFYRRPGAPDTYVRTFDEICEKIDETIA
                     LGGTGVLMQGGLHPDFGLEWYEDLLRSLRAKYPGFQLHCFSPPEIHNLHLISKLDYET
                     IMARLKDAGLYSLPGGGAEILDDEVRKRVATKCTTDEWLDVMRAVHHVGLRSTATMMF
                     GIGDKIEHRVNHLRRIRDLQDETGGFTAFIPWTFQRENTALGRKIKDEPTGVDYLKML
                     AVSRLFLDNIENFQSSWLTQGLRLGQVALRFGANDMGSIMIEENVVSAAGAHNRADEQ
                     MLQYLIREAGFTPQQRDILYEHVNRSVLSAA"
     gene            complement(6040..>6846)
                     /locus_tag="VFH96_07975"
     CDS             complement(6040..>6846)
                     /locus_tag="VFH96_07975"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HET6973948.1"
                     /translation="DFESIRVDSEEEYQAIVEFINRIQPRLVKRVKLYTKEQPILEAY
                     GVQAEIDKAIKPRVWLKSGGYLVINQTEALVAIDVNTGKFVGRGSGRLEDTITRTNLE
                     AVDEIVRQIRLRDLGGIIVLDLIDMEERRNRQRVMQALQEALRNDKSPTKVLSFNDFG
                     LVIMTRKRVKQSLERTLCSPCPYCQGAGLVKSPQTICYELLEDARRLAKEFNGDKDNK
                     QVTLRINPDVAKALRSSERDVMIEIEDYLGPIDITSDPTIHQEQFDFAVL"
BASE COUNT         1577 a         1953 c         1806 g         1510 t
ORIGIN      
        1 caagatgctg aagcttgctg taaagctcct tgcggagatc gaacatcacg tactgtccga
       61 tactgttgac gaagatcgat tggaagtaag agaagatcag acgtaacagc tgcgaggcga
      121 gatacaaacc gactaacaaa ctcaacccgt tggtttggcg cggcaggatg taccagtcga
      181 ttgcgtattg cgtaaacttc ggctgcaaca cccccaacag attaagcacc agcgtcagcg
      241 cgagagcccc aagaagcagc cgcctatacg gcttcaaata gcgcaacaac cgccgcgcca
      301 cctgaagatc gtaagtcttc ccgatcgcct cttcttcgtg atactgcttt actgctgtag
      361 acatcttagt tagttatcca cagattacgc agatgtctct aaaatatctg cgtaatctgt
      421 ggatacgttt ttaggttgca gccaactctt cctcgagtaa ctgtcgctca tgtaaatccg
      481 catactcacc accgagcgca agcaactcgt cgtgagttcc ctgctcgatg atccgcccat
      541 catccaaaac acaaatcata tccgcttcac gcacagtgga aacacgatgc gagatgatca
      601 cactagtgcg gtcgcgcatc accccacgaa gctgcccgag aatcttttct tcggtgtacg
      661 tatcgactga cgacaacgca tcgtcgagaa tcagtatctt cggctcgcgc aaaacggccc
      721 gcgcaatcgc ggtgcgttgc ttttggccgc ctgacagcgt gatgccgcgc tcaccaacca
      781 ttgcatcaaa acgatccgga aaaccggcaa tatcgtcaga aagcccagcg atctccgccg
      841 cccattcgac ctcagccatc tttgcatccg cgacaccgaa agcgatgttt cccttcactg
      901 agtcactgaa aagaaacgtt tcctgcggca cataaccaat actctcccgc agttgcgcga
      961 gcggatagtc gtgaatgtcc acttcgtcga tgaagagctt gttttccggc gcatcgatca
     1021 aacgtggaat gagattggca acagtagact tgccgctgcc ggtacggccg acaaaggcga
     1081 ccgtttgacc ttcaggaatc gtaagcgtca cgttcttgag caccggctcg tcattcggat
     1141 gataactaaa cgtcagatcg cgaaactcga tcttgccttt gatcggcggc tgcgtcacgg
     1201 tcccggggag atcggcgatc tcaggctcga tccgcagaat cgcgttgagt cgtttcaaac
     1261 tcgccgtgcc gcgctgataa agattcacga catagccgag cgcgatcagc ggccagatca
     1321 ggcgagtaag ataaagattg aacatcgtaa actcgccgac cgtgatttcg cctcgcaccg
     1381 cgagcggcac acccgcccag atgatcagta caaaaccaaa cccgatcaga aacgtcatca
     1441 acgggcgcat caaagcatcg attttgacga gaccaagatt tcgctcggca tactgccggt
     1501 tgagattgtt gaaggccgcc atctcggcgc cttcctgcgc gtacgcacgc acaacacgca
     1561 caccgctgaa gttctcctgc gcgcgcgccg tgatctggcc gaagaaatcc tgaatctttt
     1621 caaaccgcgt atggacttgc gccccgaaga atttcactgt gagcgaaacg agcggcatcg
     1681 tcacaaacag caacaacgtc agccaccagt tcagcatgaa catcaccggc aatacgaaga
     1741 tcacgacgaa gacggtgctc aacgagtaca tgatcatcgg tcccgcgagt tgccggactg
     1801 cagacatatc gttagtggcg cgtgccatca agtcgccggt gcgatgttcg tgaaaaaacg
     1861 agagcggctg attgacgaga tgacgataga actcgtcacg catgtcgtac tcgatgttgc
     1921 gcgacatgcc gatgagaatg cggcgctgca ggtagagaaa gagtccgctg atcagactgg
     1981 cgccgagaac tttggctgcc gagatggtga gcttccacca ggaaatttca gtccagtttg
     2041 cgtcaattgc ctttccgacg atgcgaggga tgagaaggcc gaccgtaaca ctgccgagga
     2101 tgcagaggat gccgagcgtc agttggagct tgtaattccg aaagtagagc gcgaacttct
     2161 ttagatcttc cacacaagga taatacttta tctgcggcaa tccgcgtaaa gcaccacttt
     2221 ggagtgcgcc aacttgttgg cgctttggta ccaaagcggt gacaagtcac cgcactccaa
     2281 aatggtgctt acattcggag atacgtgctg atcgcggtgg cggagagctt tccagcgtcg
     2341 tcgaagagct ctgctgacac ggtgatcacg cggcgtcctg cacgcaccac gcgcgccgac
     2401 gccctcgcag tcccttcact gattggccgc agataatgaa tcgtaaggtc aactgtcacc
     2461 gtgcgctcgt cctcagccag ttgcgccatg atggcaatcg ccatcgccga atcaatcaac
     2521 gtcgcgatgg cgccaccatg tgcgattcca ttgttgcgca tcagatcatc gtgcagcttc
     2581 atcgagagca ccgcgtgtcc gggcccggct gagtcgacct cgatacccag gagtttcgca
     2641 aacggaacgt gatcgagagt catgaacgat aactctgttc gggatttgcc ggctcaacaa
     2701 tcacggccgt gccgtaagcc aaaacttccg tgacgccttg cgcaagctcg gtcgattcgt
     2761 aacgtgctcc gacaatcgcg ttagcgccaa tttccgtcgc atgctggatc atgatgtcga
     2821 aggcgtgcgc acgcgtttcc tcgcacaacg tcgtccagac agtgatattc ccgcccacga
     2881 tcgtttgcag cgaggcgccg atcgtcgcga agatgtttcg ggagcgcacg acaatgccgc
     2941 gcaccactcc gagcccctgg acaacgcgat aattcggcag ctcgaacgcg gtggtcacca
     3001 tttgatgcga gacggaataa cgtggcgggc tcactgcata cggaacccgt ggttgcattg
     3061 aatcagagct catcgtttcc ttccttgcct gggtatgtgg agaaactgct gcaaatcgca
     3121 gcggccattc ttatcaaaaa caacgccttc gtattcgagc atgagttgct gcttgttgtg
     3181 tggcaacaca actctgccgg tcgagcactt gccttgcgca ttgatcacgc gatgccaggg
     3241 cgtaccttca ggcgaggagt gcatgcagaa gcctactgtt cgcggcgtgt agccctcgcc
     3301 aagcagctcg gcgatctggc cgtaagtcat cacgcggccc gatggaatgc ggttcacgat
     3361 cttgtaaacg cgctcgcggt attttgggtt ttctttatcc acagattaca cagatgttct
     3421 atccacagat tacacagatg ttctcagcac cacgcgcgct ggggcgccat cgcctttgtt
     3481 gcttcttaaa cgcagcggca aacagatcag ttcatacttg cccgccgcaa tcccggtcag
     3541 attcaagcct tcgagaatta ctacgccacg cgaaagcagt gcgagatgcg tttcgtgttt
     3601 ccctgaacca tacttctcaa tcgagagata gtcgatgccg acaagcttga ttccctgctc
     3661 gacgagccat cgcgccgccg gcagatcgag gtatgtaaag tcggtatgaa actgcggctt
     3721 ggcttcgttc cagaacgcgg aattgcgtgt cttgaatatt actcgctcag tgcctgccac
     3781 ataattcttc cgcacaaact cttcgtcgat cgcagtcgct tcttccggca cttcgatcac
     3841 cgtggcctcg ccgattagcg cgtcgagtga caacgattca accttcgtag cgccttcgat
     3901 gaaatgcgcc ggcgcgtcaa cgtgcgtgcc ggtgtgtgcg ccgaagttga gcatggaaac
     3961 attcgcgcca tcacccgctg cgaggctgca aaaatcgctg atctcgacgg caggatcggt
     4021 gggccacgtg ggcaattcgt tcgagacggg cacggtaaca tcgaaaatag gcattggcga
     4081 atagtagttt ccggtaattc ggagtgtcaa tttgatccgc gaatttgatc gaatatactt
     4141 gcagccacat gaacagtcct tttcgactcg ccgcctccag ctacttaaat tcagcgcctc
     4201 tgatttggag ctttttacac ggaccacatc gcggcaatgt ggattttgtc gaggctgtcc
     4261 ccgcgcgttg tgcgcaatta cttcgcgatt cgtccgtcga gggcgccctc gtgccggtca
     4321 tcgaatatca gcgaatcgag ggcggttcgc tcgtacagaa cgtttgtgtt ggctctcaaa
     4381 aagaagtgct cagcgtcgta ctggtttcca agaataagcg actcgaggac attcgcacag
     4441 tcgctctcga cgagtcgtct cgcacctcgg caacgctcgt caaaataatc ttcagagaat
     4501 ttctggacca cgagcccgaa tggaccacac gcgcgccaca actcgaagag atgctcgaga
     4561 aaaacgacgc agcgctgata atcggcgacc cgggcatggc ttttcctcgt cacggattga
     4621 acgtctggga catggctgcg ctctggaaac aacacacgaa actcggtttt gtcttcgcga
     4681 tgtggatggt gcgcgacgat gccggcgaac gggctcgagc ggttgatttt tcaggcgcgc
     4741 gagacgaagg agtcgcgcag atcgaagaga ttgttcgctc gtatcaggat aaaataccga
     4801 tgcgagtcga cgagttgaga aactacctga ctgaaaacat cgttttcaaa gtagacgagt
     4861 cgatggaaaa aggcttgcga ctctattttg aattggcgtt caagcatggc ttaattgagc
     4921 gcgtaaagcc gttgaggttt atctaatgtc tgccaccaac attcaaccga ttctcgatcg
     4981 cgtgctcgcc ggcgagcgaa tgactgtcga cgagtgcacg accctcctcg aatcgcacga
     5041 cctcgcccgc atgggagtcg ctgcggatga gatacgcgcg cgacgtcatc ccgacaacat
     5101 cgtcacttac atcatcgatc gcaacatcaa ctacaccaac gtctgcaacg tcgtgtgcac
     5161 gttttgcgcg ttctaccgtc gtccgggcgc acctgatact tacgtccgca ccttcgatga
     5221 aatctgcgag aagattgacg agaccattgc gctcggcggc acgggcgttt tgatgcaggg
     5281 tggattgcat cctgacttcg gcctcgagtg gtacgaagat ctgcttcggt cgctgcgcgc
     5341 aaaatatccg ggatttcaat tgcactgctt ctcgccgccg gagattcaca acctccattt
     5401 gatctcgaaa ctcgattacg aaacgatcat ggcgcgcctc aaagacgcag ggctctacag
     5461 cctgcccggt ggcggcgccg agattctcga cgacgaagtg cgtaagcgcg tcgcgacgaa
     5521 gtgcacgact gatgaatggc tggacgtgat gcgcgcggtg caccatgtcg gtctgcgctc
     5581 aacggcgacg atgatgtttg gtatcggcga caagattgag catcgcgtca accacctgcg
     5641 acgcattcgc gacttacagg atgaaaccgg cggcttcaca gcgttcattc cctggacgtt
     5701 tcaacgcgag aacaccgcgc tcggacgcaa gatcaaagac gaaccaactg gcgtggatta
     5761 tctgaagatg ctggcggttt cgcgcctctt cctcgacaac atcgaaaact ttcagtcgtc
     5821 gtggctgacg cagggtttgc gtctcgggca agtggcgttg cgttttggag cgaacgacat
     5881 gggctcgatc atgattgagg aaaacgtcgt gtcagccgcg ggcgctcaca atcgagccga
     5941 cgagcaaatg cttcaatatt tgattcgcga agctgggttc acgccccagc aacgcgacat
     6001 cctctacgaa cacgtgaaca gatccgtttt atccgcggct taaaggaccg cgaaatcgaa
     6061 ctgctcttga tgaattgtcg gatcactcgt gatgtcgatt ggtccgagat agtcctcaat
     6121 ctcgatcata acatcacgtt cactcgagcg tagcgcttta gccacatcgg gattgatccg
     6181 caaggtgacc tgcttgttgt ccttgtcgcc gttgaactcc ttcgccaacc ggcgcgcatc
     6241 ttccaacaac tcgtaacaga tcgtctgtgg cgacttcacg aggccggcac cctgacagta
     6301 cggacacggc gaacacagcg tgcgctccag cgattgcttc acgcgcttgc gcgtcatgat
     6361 cacgagaccg aaatcgttga acgaaagcac cttcgtcggc gacttgtcgt tgcgcaaagc
     6421 ttcctgcaac gcctgcatca ccctttgccg gttccggcgc tcttccatgt cgatcaggtc
     6481 gagcacgatg atgccgccca gatcgcgcag tcgaatctgt cgcacaatct catccactgc
     6541 ttcgaggttg gtgcgcgtga tcgtgtcttc gaggcgaccg ctgccgcgcc cgacaaactt
     6601 gccggtgtta acgtcgatcg cgacgagcgc ttctgtctga ttgatcacga ggtagccgcc
     6661 cgacttcagc caaacacgcg gcttgatcgc tttatcaatc tcagcctgca cgccgtacgc
     6721 ttcgagtatc ggctgttctt tcgtgtagag cttgacgcgc ttcacgagtc gcggctgaat
     6781 gcgattgatg aactcgacga tcgcctggta ttcctcttcg gaatcaaccc ggatcgattc
     6841 aaaatc
//
LOCUS       DASSFQ010000220         5090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1433133, whole genome shotgun sequence.
ACCESSION   DASSFQ010000220 DASSFQ010000000
VERSION     DASSFQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433133"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1758)
                     /locus_tag="VFH96_07980"
     CDS             complement(58..1758)
                     /locus_tag="VFH96_07980"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6973949.1"
                     /translation="MNISRRQFLSNSGKAAVAVTLLHSFVPARALAQAAAVQPLFARL
                     DEFITRHMHETGAPGMTLALANRDGLIRVSTYGYADIKAGSRVVPETMFEIGSISKSF
                     VGLALMQLRDEGKLDLNKPIVEYLPWLKINSKFAAITTHHILTHSAGLPGAPLLLDAL
                     LGELWTAYEPGKRFLYSNTGYNILGFLIAAIDKRPFADAMRARMIEPLGMSASSPIII
                     NDLRKLMAIGYEPLQEGKPYPVNGPLAEAQWLEVDIAAGSVASTPADMAKYMRMLLNR
                     GALPKGRLISEESFALLTKPLIDSPYRGEPAGYGYGLWVSDIAGHTRLRHTGGMVAFS
                     SSIDVDVTGNIGAFASVNANLRGYRPVAVTKYAVELFNASADNKPLPEAPAPPPSPIE
                     VKNVADYVGTYTTLDGKKLEFVAEGTKLVLVYGNRRVVLERSGGDRFIVKHPDLDTYL
                     LGFVRENQQVTQAYHGANWYLGEKYTGPKTFETKKEWEGFAGHYNNDSPWYGDTRIVM
                     RKGQLYIEGVQPLVPRGDGKFGIGDTEAPDWVTFESIVDGRAMRLNFSGIVFRRVFTP
                     "
     gene            complement(1794..4202)
                     /locus_tag="VFH96_07985"
     CDS             complement(1794..4202)
                     /locus_tag="VFH96_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6973950.1"
                     /translation="MLGQLLLHYRVVEKIGEGGQGTVYKVLDTTLDRPAVIKVLPPDL
                     TDSASNLVRFEREAKLASSLDHPNICTIFGLHKVGDVQFIAMQFIEGRNVREIVDGKP
                     LDLRRALSIAIQVTDALAAAHSRGIVHRDIKARNVMVTRSGTVKVLDFGLAKLIESPQ
                     SAPNDPQLTEMGVPYGTSTYAAPEQAQGLKADHRADIFSTGVLLYEMLAGTWPFRGKT
                     ALDVRYAVVYHKPKPIVGARGEDSAPLRRIQEILDKALAKAPQDRYQKIEDMRADLQE
                     VLREIEVDTSVGRTLTDAPATTTVPRLITTNTFWTTPRKITVGALALLVVAALALLAF
                     RRNPPAESDSTISSLAVLPFTNSDPNIEYLSDGITESLIDSLSRMPNLKVKSSSTVFH
                     YKGRETDPKKIGRELGVHALLSGTVSQVGDDLSVSVELIDVRDDSHIWGERYGRKVSE
                     VVALPQQISRDVSQRLRSRTDNLDHAQLTRNYSPDSEAYRLYLQGRYNWNKRTVQGLQ
                     NGIEYFGKAIQRDQDYALAYAGLADCYLLLNVYNVTSADESFPKAEAASRKALSINES
                     LAEAHTALGFVTYRYHLKWAEAEEHFQKAIALNPNYATAHQWYGSYLAASGRLNEAVV
                     EAKIAHDLEPFSLTIYSDYIRALYYAGRLDEARKESLKLMETDPSFARAHYELGLVAE
                     EEGKFEEAINEFKLGLKALPDNVAALTALGHAQARAGKRADAEKAIARLQELSKRQYV
                     SPFQTAVIYAGMDERKLALDWLEKSRDERFNWLPFIAVDPVFKNLRVEPRFVELSKSM
                     GLTK"
     gene            complement(4470..5027)
                     /locus_tag="VFH96_07990"
     CDS             complement(4470..5027)
                     /locus_tag="VFH96_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973951.1"
                     /translation="MKLSLLGLVFGLLFSFASQVTGINDKQVNDTIKQKGNELEVSLV
                     ADKHSYKPGDRIKLGVTVINSNAVKDLFVYGTLQFGPRASFTLFRRDAKGMEVPTRFI
                     DDAWELPPKANDRSAFVKLLPFHFLGTTYSSSIHLLNMERPGKYTMWVEYHCPLSVEN
                     VEVDPFWGKESGTVKSNVIEIEVKP"
BASE COUNT         1103 a         1425 c         1381 g         1181 t
ORIGIN      
        1 ggtcaccatg tgaggagatg gtccacgcgc acagagttga gtcgttactg atgtaatcta
       61 tggcgtaaat actcggcgga agacgattcc tgagaagttg agccgcatcg cgcgaccatc
      121 aacaatcgat tcgaaagtga cccaatccgg cgcctcggta tcgccgattc caaacttccc
      181 atcaccacgc ggcacgagcg gctgcacgcc ctcgatgtag agctgtccct tgcgcatcac
      241 gatacgcgta tcgccgtacc agggactgtc gttgttgtag tgaccagcaa aaccctccca
      301 ctctttcttc gtctcgaacg tcttcggccc ggtgtacttt tcgccgaggt accagttcgc
      361 gccgtgatac gcctgcgtca cttgctggtt ctcgcgcacg aagcccagca gatacgtatc
      421 cagatcggga tgcttcacga tgaagcgatc gccgccggag cgctcgagta cgacgcgacg
      481 attgccgtac acaagaacga gcttggttcc ctcagccacg aactcgagct tcttgccgtc
      541 gagcgtagtg taagtgccaa cataatcggc tacgttcttt acctcgatcg gcgaaggcgg
      601 cggtgccgga gcttcgggca gcggcttgtt gtctgccgac gcattgaaaa gctcgacggc
      661 atacttcgtc accgccacag gacgatagcc acgcaggttc gcgttgactg acgcaaacgc
      721 gccgatgttt ccggtcacat caacgtcgat cgacgagctg aaagcaacca tgccgccggt
      781 gtgacgcaaa cgcgtgtgtc cagcgatgtc gctcacccac aatccgtaac cataccccgc
      841 cggttcacca cgatacggtg agtcgatcaa cggcttcgta agcaacgcaa aactctcttc
      901 agagatcaaa cggcccttcg gtagtgcacc gcgattgagc agcatgcgca tgtatttcgc
      961 catgtcggcg ggcgtcgagg cgacactacc cgccgcgatg tcgacttcga gccactgcgc
     1021 ttcagcgagt ggaccattaa ccggatacgg cttgccttcc tgaagcggct cgtaaccgat
     1081 ggccatcagc ttgcgcagat cgttgatgat gatcggcgag ctcgcagaca ttccgagcgg
     1141 ctcgatcatg cgcgcacgca tcgcgtccgc gaaaggccgc ttgtcgatcg ctgcgatcag
     1201 gaaaccaaga atgttgtagc cggtgtttga ataaagaaaa cgcttgccgg gctcgtacgc
     1261 ggtccaaagc tcaccgagca acgcgtcgag caggagcggc gcgccgggca gtccggccga
     1321 atgcgtcaag atgtgatggg tagtgatcgc cgcaaacttg gaattgatct tcaaccacgg
     1381 caggtattca acgatgggtt tgttgagatc gagttttccc tcgtcgcgca gttgcatcaa
     1441 tgcgagaccc acgaacgact tcgagatcga tccgatctca aacatcgtct ccggtacgac
     1501 acgcgagccg gctttgatgt cggcgtaacc ataagtcgag actctgatca aaccgtcgcg
     1561 gttcgcgagg gcaagcgtca ttccgggcgc gcccgtctcg tgcatgtgcc gcgtgataaa
     1621 ttcatcgagc cgcgcgaata aaggctgcac cgccgccgcc tgggccaacg cacgcgccgg
     1681 cacgaacgag tgcaaaagcg tcaccgccac cgcggccttc ccactatttg agagaaattg
     1741 tcttcgtgaa atattcatac agatccgtct tcaatccgtt ctatccgtgt ttactatttc
     1801 gtcaaaccca tgctcttcga taactctaca aaccgcggct ccacgcgcaa attcttgaac
     1861 actggatcga ccgcaatgaa cggcaaccag ttgaaccgct cgtcacgcga cttctcgagc
     1921 cagtcgagcg cgagcttgcg ttcatccatg cccgcataaa ttaccgcagt ttgaaagggc
     1981 gaaacgtact gccgcttcga taactcctga agccgcgcga tcgccttctc ggcatcggct
     2041 cgcttccccg cgcgcgcctg cgcatggccc aacgccgtca gtgccgccac gttgtcaggc
     2101 aatgctttca atccaagctt gaactcgttg atcgcttcct cgaacttgcc ttcctcttca
     2161 gcgacgaggc cgagctcgta atgcgcgcgt gcgaagctcg gatcggtttc catgagtttt
     2221 agcgactcct tgcgagcttc gtcaagccgg cctgcataat acagcgcgcg gatgtagtcg
     2281 ctgtagatcg tgagtgagaa cggctcgagg tcgtgcgcaa tcttcgcctc gacgactgct
     2341 tcgttcaatc tgccgctcgc ggccagataa ctaccgtacc actggtgcgc agtcgcatag
     2401 tttggattga gcgcgatcgc tttctggaaa tgttcctctg cctcggccca cttcagatgg
     2461 taacgataag taacaaagcc gagcgcggta tgagcttcag cgagactctc gttgatcgac
     2521 aacgccttcc gtgaagcagc ttcagctttc ggaaaagatt cgtcagccga cgtaacgtta
     2581 tagacgttga gtagcaggta acaatcggcg agtccggcat aagcgagcgc gtaatcctgg
     2641 tcacgttgga ttgctttgcc gaaatattca atgccgtttt gcagtccctg caccgttcgc
     2701 ttgttccagt tgtaacgacc ctgcaggtag agacgatacg cttcagaatc gggtgaatag
     2761 ttgcgcgtca attgcgcgtg gtccaggttg tccgtgcgcg agcgcagtcg ttgcgatacg
     2821 tcacgtgaga tctgttgcgg cagcgccacc acttccgaga ctttgcgacc gtaacgctca
     2881 ccccagatat gactatcgtc acgcacgtcg atcagctcga cactgactga cagatcgtcg
     2941 ccgacttgcg aaacggtgcc tgataacaac gcgtgtacgc cgagctcgcg tccgatcttc
     3001 ttcggatcgg tctcgcgtcc tttgtaatga aagacggtgc tgctcgactt cactttcaga
     3061 ttaggcattc tcgaaaggct gtcgatcagg ctttctgtaa tgccgtctga aaggtattcg
     3121 atgttgggat cgctgttggt gaacggcagg acagcgagcg agctgatcgt cgaatcggac
     3181 tcagccggcg ggtttcttcg aaacgccaag agcgccaggg cggcaaccac gagcagggcc
     3241 aaagcgccaa ccgtgatctt gcgtggcgtg gtccaaaagg tgtttgtagt gattagacgc
     3301 ggtacggttg ttgtcgcagg agcgtcggtc aacgttctcc cgacgctcgt atcgacttcg
     3361 atctcacgca gcacttcctg aagatcggcg cgcatatctt cgatcttctg gtaacgatcc
     3421 tggggcgcct tcgcgagcgc cttgtcgagt atctcctgaa tacgacgcag cggggccgag
     3481 tcttcaccac gcgctccgac gatcggtttc ggcttgtgat agacgacggc gtaacgcacg
     3541 tcgagagcag tcttgccgcg aaagggccac gtgcccgcga gcatttcgta aagcaaaaca
     3601 ccggtagaaa aaatgtctgc gcgatgatcg gctttcaagc cttgcgcctg ctcgggcgcg
     3661 gcgtaggtcg aggttccgta aggcacgccc atttccgtaa gctgcggatc gttcggcgcg
     3721 gattgtgggc tctcgatcag ttttgcaaga ccgaagtcga gcaccttcac cgttcccgag
     3781 cgcgtgacca tcacgttgcg cgccttgatg tcgcgatgga cgatgccgcg actgtgcgct
     3841 gccgcgagcg cgtcagtcac ttggatcgcg atcgagagtg cgcgtcgcaa atcgagtggt
     3901 ttgccgtcga cgatctcgcg cacgttgcgt ccttcgatga actgcatcgc gataaactga
     3961 acgtcgccga ctttatgcag accgaagatg gtgcagatgt ttggatgatc gagcgaagac
     4021 gcgagcttgg cttcgcgttc gaaacgaacg aggttggaag cgctgtcagt aagatccggc
     4081 ggcaggactt ttataaccgc gggccgatcg agcgtcgtat ccaagacctt gtaaacagta
     4141 ccctgtccac cctcgccgat cttctcgacg acacgataat ggaggaggag ttgcccgagc
     4201 ataggagaat ggggaacgtt tagcttccgg tgcgggcgta ttctggacag gattcgcgga
     4261 atttacaaga ggcacggtgg agattagaca gagttatggg attttcaaga gcatctcgtt
     4321 tatcttgaaa atcctgtaat catgtctttt caattcactg ggttgattca attaaattta
     4381 atttgtaata accgcgtccg tcgtagcttt tgcgcgtgac ctcaaatcga ctttcaggcg
     4441 aataagatct gtatgagtct gtatctggct catggtttaa cttcgatttc gatcacattc
     4501 gatttgaccg tgccgctctc ctttccccaa aagggatcca cttcaacatt ttcaactgag
     4561 agagggcagt gatactcaac ccacatcgtg tactttcccg gccgttccat atttagcaga
     4621 tgtattgacg agctgtaggt ggttcctaga aagtgaaaag gaagcagttt cacaaatgcg
     4681 cttctatcgt tagctttcgg tggaagctcc caagcgtcat ctatgaatcg tgttggtact
     4741 tccattcctt tcgcatcgcg acgaaaaagc gtgaagctgg cacggggacc gaactgaagc
     4801 gtgccataga cgaataagtc tttgaccgca ttgctgttaa ttacagtcac tccaagtttg
     4861 attcggtcgc ctggcttgta ggagtgctta tcagcgacaa gtgacacttc caactcattg
     4921 cccttctgct tgatggtgtc atttacttgc ttgtcgttaa ttccagtcac ttgacttgca
     4981 aagctgaaga gcaatccaaa gactagcccg agaagcgata gtttcattgc gcttattctc
     5041 ctccattcac ttacgaagct tttgagggtt gttcatgatc gctagcactt
//
LOCUS       DASSFQ010000221         5380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_407156, whole genome shotgun sequence.
ACCESSION   DASSFQ010000221 DASSFQ010000000
VERSION     DASSFQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407156"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFH96_07995"
     CDS             complement(<1..192)
                     /locus_tag="VFH96_07995"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973952.1"
                     /translation="MPPESISARVKESFPSDEDRQFDLTLRPTRIDEYIGQRQVKENL
                     RVYMKAARNRREALDHVLLT"
     gene            complement(200..820)
                     /gene="ruvA"
                     /locus_tag="VFH96_08000"
     CDS             complement(200..820)
                     /gene="ruvA"
                     /locus_tag="VFH96_08000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6973953.1"
                     /translation="MIAHLSGTLLSKQATSVIVDVSGVGYEVNIPLSTFYDLEDLGST
                     VQLRIYTHVREDAIQLYGFKTARERELFLKVISVSGIGPKLGITLLSGMSADEMIASI
                     RTNNLARLTLIPGVGRKTAERLILELREKVADLSSAQLEEELGAKPEAGEPSEDTVRA
                     DALSALLNLGYQRSAAEKAIDAALGEGGDVSVESILRRSLKKLARV"
     gene            857..1618
                     /locus_tag="VFH96_08005"
     CDS             857..1618
                     /locus_tag="VFH96_08005"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET6973954.1"
                     /translation="MTYKTYRAILAADRFRRMKSEKISELTTSRLSVYLRCLNALHSA
                     GIKTISSQALAEQFNLNSAQIRKDLGYFGEFGVRGVGYYVDDLREHITKILGLDTPHR
                     VGIVGAGRLGTALANYNGFDKSNFTVVALFDNDRQKIGRRIGDAKLLVHDVDQISNII
                     RAEAIDVMVIAVPAKAAQRVLNQIMSAGIKAVLNFAPASLISRRGVKVKTVDLTTSLE
                     SLSYFLSQPSGTASHAKTQKRKSDISWMSRQSNAI"
     gene            1588..2346
                     /locus_tag="VFH96_08010"
     CDS             1588..2346
                     /locus_tag="VFH96_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET6973955.1"
                     /translation="MDEQTIKRDLIRVCHLMYERSYVVSSDGNVSVRLDDGRILATPT
                     MTCKGRMTEDLIAITDLEGKALNDRKASSELAMHLLIYRERPDVKAVCHAHPPHGTAF
                     AVAGLAIDQPILSEVILTLGCVPLAAYGTPSTEELTREMQPFVQHHNALLMANHGAVA
                     YGADLWQAWDRLETLEHTAKIAILSRALGGSKNLPADAIEKLINVREKAGYLGESARG
                     QACGYLNPSENGDAKISLTREELVELLSQAVNGK"
     gene            2381..2662
                     /gene="eutM"
                     /locus_tag="VFH96_08015"
     CDS             2381..2662
                     /gene="eutM"
                     /locus_tag="VFH96_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HET6973956.1"
                     /translation="MQEALGMVETKGLVAMIEAADAMVKAANVTLVGYEKIGAGFVTA
                     IVRGDVAAVKAATDAGAAAARRVGELVSVHVIPRPHGSVDETLPIGLDR"
     gene            2668..2940
                     /locus_tag="VFH96_08020"
     CDS             2668..2940
                     /locus_tag="VFH96_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET6973957.1"
                     /translation="MIIARILGTVVSTQKDERLTGKKLLIVRPINVDGSDTTGYVVAV
                     DTVGAGFHERVLVVAGSSARLAQGMKDVPVDAAIVGVIDTVDVVQN"
     gene            2943..3215
                     /locus_tag="VFH96_08025"
     CDS             2943..3215
                     /locus_tag="VFH96_08025"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET6973958.1"
                     /translation="MQLARVIGTVVATVKNDSLDGRKLLIVQTLDRNLEPSGKPMVAV
                     DAIGAGVGELVFWCRGKEASFPFKREDTPTDCTIIGIVDSEQHVTR"
     gene            3212..3487
                     /locus_tag="VFH96_08030"
     CDS             3212..3487
                     /locus_tag="VFH96_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET6973959.1"
                     /translation="MILARVVGNIVATQKNRRYESARVMLCQQITPEGEETSYSTIAL
                     DSVDAGVGDTVLIVQEGWSASTAATGEAGAAIDSAIVGVVDRIDLLT"
     gene            3488..3820
                     /locus_tag="VFH96_08035"
     CDS             3488..3820
                     /locus_tag="VFH96_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973960.1"
                     /translation="MEDQARLMAERIARRVSAKPVAPQSSDIGAELAGIRASLNELQD
                     RLVQIEAKVSGPRVHSPWSHPSMERFGVEEATVSELVDFFQGEKQCSIEPGGKPCDHC
                     AMCSSRGF"
     gene            complement(3877..4506)
                     /locus_tag="VFH96_08040"
     CDS             complement(3877..4506)
                     /locus_tag="VFH96_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET6973961.1"
                     /translation="MKTRRLVMLVGLVLIAFAASGCGYNTLTTKQQDVKAKWANVETQ
                     LQRRGDLLNNLAESAKMAGIQEQEVFGQIAAARSRLLNAQNQAPQGEGGDKSPEQKQA
                     VIDAANSFGGTIGRLLVLQEAYPTLKSNENFLKLQDEVAGTENRVATARTDYNASVQD
                     YNTTRARFPTVISAKLFGFKEEPFFKADEAAKQVPQLNANSLRNNQNSK"
     gene            complement(4530..5276)
                     /locus_tag="VFH96_08045"
     CDS             complement(4530..5276)
                     /locus_tag="VFH96_08045"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET6973962.1"
                     /translation="MIDMVKTAVKEALNGLVEDLRATHGANLASVVLYGSAAAGDHIE
                     LRSDYNILIALNRITPEDLRQAQAPMREWQRLGHPLPVYFTVEELSDAADVFPIEFHQ
                     MANARVVLYGQDPFEFVKLSDENLRHQAEYELRSKLIQLRRHYIPASVSVDKLCALMS
                     DSLSSFASLFRAVLMLHGHEAPISKPDVVRATAKLLQLDPAPFERIFEFRANDRLPSS
                     EKEANDLFGAYLLQIEQVVEAVDQLGTATQ"
BASE COUNT         1183 a         1416 c         1573 g         1208 t
ORIGIN      
        1 ggtgagcagc acgtggtcca gcgcttcgcg acgattgcgc gcggctttca tgtagacgcg
       61 caggttctcc ttgacctgac gctggccgat gtattcgtcg atacgagtcg ggcgcagcgt
      121 gagatcgaat tgccgatcct cgtcgctggg aaaactctct ttcacgcgcg ctgatattga
      181 ctcaggaggc atgttgattt caaacccttg cgagtttctt cagactgcgt ctcaggatcg
      241 actcgacgct gacgtcgccg ccttcgccga gcgcggcgtc gatcgctttt tccgcggccg
      301 agcgttggta accaagattg agcagggccg aaagcgcgtc ggcgcgcacc gtgtcttccg
      361 aaggctcgcc agcttcgggt ttcgcgccca gctcttcctc gagttgagca gacgataggt
      421 ccgcgacttt ctcacgcagc tccaggatca ggcgctcagc ggttttgcgt ccgacgccgg
      481 ggatcaacgt tagtcgtgcg agattgttgg ttctgattga tgcgatcatc tcatcggcgc
      541 tcatgccgga aagcagcgtg atgccgagtt tggggccgat gccgctcacc gaaataacct
      601 tcagaaacaa ctcacgctcg cgcgcggtct tgaagccgta aagctgaatc gcatcttcgc
      661 ggacgtgtgt gtagatgcga agctgaaccg ttgagccgag gtcttccaga tcgtagaaag
      721 tcgagagcgg gatgttgact tcatagccga cgccgctgac atcgacgatc acggaagttg
      781 cttgtttcga gagcagtgtg ccgctgagat gcgcaatcat gcgggcggga ttgtaacaga
      841 aaccgatagg acagatatga cctataagac ctatcgcgct atacttgctg ccgacaggtt
      901 ccgccgcatg aagtccgaga aaatatcaga attaacaacg agtcgcttga gtgtttacct
      961 tcgttgcctc aatgcgcttc acagtgccgg aatcaaaacc atctcgtcgc aggcgctggc
     1021 tgagcagttc aatctcaact cggctcagat cagaaaagat ctcggttact tcggcgagtt
     1081 tggcgtgcgt ggtgttggtt actacgtcga cgacctacgc gagcacatca cgaagatact
     1141 cggtctcgac acgccgcatc gggtcggcat cgtcggcgcg ggtcgcttgg gtaccgcact
     1201 cgcaaactac aacggcttcg acaagtcaaa cttcacggtg gtcgcactgt ttgataacga
     1261 tcgacaaaag attggccggc ggattggcga cgcgaagctg ctcgtacacg atgttgacca
     1321 gatctcgaac atcattcgtg cggaagcgat cgacgtgatg gtgattgcgg tgcctgcgaa
     1381 agcggcgcaa agagttctca accagatcat gtcggcaggg attaaagcgg tgctgaactt
     1441 tgcgccggcc tcgttgatct cgcgacgcgg cgtgaaggtc aagacagtcg atctgacgac
     1501 gtcgctggaa tcattgtcgt attttctatc tcagccttcc ggcacagcgt ctcatgcaaa
     1561 gacgcaaaag cgcaaaagcg acatttcatg gatgagcaga caatcaaacg cgatctaatc
     1621 cgggtttgcc acctgatgta cgagcggagc tatgtcgttt cgtctgacgg taacgtgagt
     1681 gtgcgactcg acgacggccg catcctcgcg acgccgacaa tgacgtgcaa gggacgcatg
     1741 accgaggatc tgatcgccat taccgatctc gaaggcaaag cgctgaacga tcgcaaagcg
     1801 tcgagtgagc ttgcgatgca tcttttgatc tatcgtgagc gtccggatgt gaaggctgtt
     1861 tgtcacgcac atccgccaca cggaaccgcg ttcgctgtcg ccggtctggc aatcgatcaa
     1921 ccgattcttt cggaagttat cctgacgctc ggctgcgtgc cgctcgctgc ttatggcact
     1981 ccatcaacag aggaattaac gcgggagatg cagccattcg tacaacatca caacgcgctt
     2041 ttgatggcta atcacggagc ggtcgcttac ggcgcggatc tgtggcaggc gtgggaccgt
     2101 ctcgagacgt tggaacacac ggcgaagatc gcgatcctct cgcgcgcgct tggcggttcg
     2161 aaaaatctgc ccgctgatgc gattgagaaa ttaatcaacg tccgtgagaa ggcgggctat
     2221 ctcggcgagt cagcgcgcgg ccaggcgtgc ggttatctaa atccttcgga aaacggcgat
     2281 gcgaagattt cactcacgcg tgaagagctg gtcgaattgt taagtcaggc agttaatggt
     2341 aaataatccg ttgttaaaaa cttctgttag gagtatctga atgcaagaag cactcggaat
     2401 ggttgaaaca aaaggcctcg tcgcgatgat cgaagccgcg gacgcgatgg tgaaggccgc
     2461 gaacgtcacg cttgtcggtt acgaaaagat cggcgccggc tttgtgactg cgatcgtgcg
     2521 tggcgatgtg gctgcggtca aagccgccac tgacgcaggc gccgccgccg cgcgacgcgt
     2581 gggcgagctc gtcagcgtac acgtgattcc gcgtccgcat ggcagtgtcg atgagactct
     2641 tccgatcggt ctcgatcgct aaccagaatg atcattgctc gcatcctcgg gactgttgtc
     2701 tccacgcaaa aagacgaacg gctgaccgga aagaagttgc tgatcgtgag accgatcaat
     2761 gtagacggca gcgacaccac cggctacgtg gttgcagttg acacagtggg cgccggattt
     2821 cacgagcgtg ttctcgtcgt tgcgggatcg agtgcgcgac tggcccaggg gatgaaagat
     2881 gtgcctgttg atgcggctat tgttggcgtg atcgatactg ttgacgtggt ccagaattaa
     2941 agatgcaact cgcgcgcgtg atcggcacag tcgtggcaac agtgaagaac gattcgctcg
     3001 acgggcgaaa gctgctgatc gtgcagacgc tcgatagaaa tctcgaaccg tcaggcaagc
     3061 cgatggttgc tgtcgacgcg attggcgcgg gtgttggaga gctggtgttc tggtgtcgcg
     3121 gcaaggaagc gagtttccca ttcaaacgag aagacacgcc gactgattgc acgatcatcg
     3181 ggattgtcga ttcggagcag cacgtaacca gatgattctc gcacgcgtgg ttggaaacat
     3241 cgtcgcgacg caaaagaaca ggcgttacga gagcgcgcgt gtgatgttgt gccagcagat
     3301 cacgccggaa ggtgaggaaa cgagttattc gacgatcgcg ctcgattcgg ttgatgcggg
     3361 tgttggtgat acggtgctaa tcgttcagga aggatggagc gcatcgactg ccgcgactgg
     3421 cgaggcgggc gcggcgattg attcagcgat cgttggagtt gtagatcgga tcgatctctt
     3481 aacgtaaatg gaagatcaag caagattgat ggcggagagg atcgcgcggc gcgtgtcggc
     3541 aaaacccgtt gcgcctcaaa gcagcgacat cggtgcggag ctggctggaa ttcgcgcgag
     3601 tctcaatgag ctccaggatc gattggttca gattgaggct aaggtaagtg gtccacgcgt
     3661 gcactcgccg tggagtcatc cgagcatgga gcgcttcggt gtggaagagg cgacggtgtc
     3721 tgagctcgtt gatttcttcc agggtgaaaa gcagtgcagt attgagcccg gtgggaagcc
     3781 gtgtgaccac tgcgcgatgt gtagctcgag gggattttag ccacggataa cacggataaa
     3841 aatccggatc aaatccgtgt tatccgtggc tggtttttat ttgctgtttt gattgttccg
     3901 cagtgaatta gcgttgagct gcggtacctg cttcgccgct tcgtcagcct tgaagaacgg
     3961 ctcctccttg aatccgaaaa gcttcgctga gatcactgtc ggaaagcgcg cgcgcgtagt
     4021 gttgtagtcc tgcacgctcg cgttgtaatc cgtgcgcgca gtggccacgc ggttctcggt
     4081 gcccgcaacc tcatcctgta acttcaaaaa gttttcattt gatttcagag ttggatacgc
     4141 ttcctgcagc accagcaaac gcccgatcgt gccgccgaaa ctgttcgcag catcgataac
     4201 ggcttgtttt tgttccggtg atttgtcgcc accctcacct tgcggagcct ggttctgggc
     4261 attgagcagc cgcgaacgtg cagcggcaat ctggccgaat acttcctgct cctgaatgcc
     4321 cgccatcttt gctgactcgg caagattatt taaaaggtcg ccgcgacgtt gaagttgggt
     4381 ttcaacgttg gcccattttg ccttcacgtc ctgttgcttt gtcgtcaggg tgttgtaacc
     4441 acaaccactc gccgcaaaag caatcagcac aaggccgaca agcattacta atcttcgcgt
     4501 cttcatctca aatctcccat gtgtaaaagt tactgtgttg ccgttccaag ctggtcaact
     4561 gcttcaacta cctgctcgat ctgaagcagg taagctccga aaagatcatt cgcttctttt
     4621 tctgacgagg gaagacgatc gtttgcgcga aactcaaaga ttcgttcaaa gggcgctggg
     4681 tcgagttgca acagtttcgc tgtcgcgcgc acaacgtcgg gtttggaaat cggtgcttcg
     4741 tgaccgtgca acatcaacac ggcccggaag agcgatgcga aactcgacaa gctgtcgctc
     4801 atcaaagcgc acaacttgtc gactgaaacc gaggcgggga tgtaatgacg acgtaactga
     4861 atcaacttgc ttctcagctc gtactcagcc tggtgacgca ggttttcatc agagagtttt
     4921 acaaactcaa aaggatcctg gccgtaaagc accacgcgcg cgtttgccat ctggtgaaac
     4981 tcgatcggaa aaacgtccgc cgcatccgag agctcttcga cagtgaaata gacgggcaat
     5041 ggatggccca accgttgcca ctcacgcatc ggcgcttgcg cctggcgcaa gtcttccggc
     5101 gtgatgcggt tcagcgcgat caggatgttg tagtcggagc gtagctcgat gtgatcgccc
     5161 gccgcagcag agccgtagag tacgactgac gcgaggtttg ctccgtgcgt tgcgcgcaga
     5221 tcctcgacga gtccgttgag agcttctttt actgcagtct taaccatatc aatcaccgtt
     5281 ttttagccac agattacacg gattgaaatc cgtgttacca actgccaccc gcgcctccgc
     5341 caccaaagtc gccgcctccg ccaaaaccgc caccaaatcc
//
LOCUS       DASSFQ010000222         5762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_251993, whole genome shotgun sequence.
ACCESSION   DASSFQ010000222 DASSFQ010000000
VERSION     DASSFQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251993"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFH96_08050"
     CDS             <1..661
                     /locus_tag="VFH96_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6973963.1"
                     /translation="VRFYDYASEVIPNENPEVKAEVLIKAADLFARSKTTNDLNNALA
                     RYSTAIKLLASTDPQQITINLRIGDAQYASHRIQEAREAYQKAAELARAAKNSADYGE
                     ALRRIGDTYVSPEKENAKALEAYNRALAAYGVQDPTSSGVNLVFGDARVREAIGLVNE
                     QSGNKTQADSFYRQAKKLYSDLLKRSTRDFDEFPSMRVEISSRLDGVTNSINRLQVSP
                     R"
     gene            765..840
                     /locus_tag="VFH96_08055"
     tRNA            765..840
                     /locus_tag="VFH96_08055"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:798..800,aa:Ala,seq:ggc)
     gene            1018..3447
                     /locus_tag="VFH96_08060"
     CDS             1018..3447
                     /locus_tag="VFH96_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6973964.1"
                     /translation="MDVLWKDLVYSARMLWKKPSLTAVAIIAIGLGIGANTAIFSVVN
                     TVLLQPLPFPQPKQLVNIASEQRNQTLDGRGTSSVPDFLDVQKSSTTLQYVATYQGSG
                     TFTTDGNEPERLLGAAVSYDYFNVLGVKPELGRVFTPDEDKPGAASVVLISHALWQRR
                     YAGDPNIIGREIDLGGKTTVVGVMPAGFEFPISDDNQDYWEPLFSAAFMTKEIRDERA
                     NRFLPVIGRLKPGATVAQAKADLDLISRQIEQSSPVSNTNVIFNTVSMHEDVTRDYRT
                     ALLVMLGAVGLVLLIACANVANLLLARAVARQKEIAIRMALGASRRRIAGQLLMESVL
                     LALAGGAFGLLLATWGTDLLVAYGPADVPRLREVGVDRYVLFFTLGLATLTGIVFGLA
                     PALHAAKPAPGNMLKENGRGVYTGRNRMHSALIVSEVALSLMLLAGAGLLINNFWRLL
                     RTDAGFNPKGVLALDIPLSRETYKTTEERVAAFQQLIARMKTVPGVRDVSVVSNVPLS
                     ENDIEVSFQIEGREPYKPGEEAVADYTVAGNDYFRTMDIALLRGRVFTDSDTANAPSV
                     MVISKAFAKVYFPNEDAIGKRIILDGPNKTPREIVGVVDDVRRKGLDSDVQPEMYFSY
                     VQRPERRLNLVLRSTTEDASQLTPAARAEVKAFNPRQIIWRAQTLEQMLSTSVAPRKF
                     NMMLLGIFAGVALVLAAVGLYGVMSYSVSWRTQEIGIRMALGAKRTDVLRMVVRQGMT
                     MTLIGLALGLVGVIALSRVMVGLLYGVSPTDPLTFTGVSIVLLLVALLACLIPARRAT
                     RVDPIVALRSE"
     gene            complement(3509..>5762)
                     /locus_tag="VFH96_08065"
     CDS             complement(3509..>5762)
                     /locus_tag="VFH96_08065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973965.1"
                     /translation="DGEIERLRWTLASLNANAPHDVELLLLLDGIALNETTMRALPAA
                     NRLRLSGTSDARGTAACFNRLASLTDADLLVLLESGVQVAAGWLDRLLAALDADPRNG
                     LAGPSTNNSWNEQCAFPRCSGNTAQIIAAANEASKRFGSEVRTLEPLYSLGDFCYAVR
                     REVLEAIGAADESYALGPCWEMDYNIRAARAGWRGVWACSAFVHRPPYSARRRIEEGR
                     RFQKNKKLYQDKFCGARLRGDKTDYREHCRGDACPNFAPGGLIEIKRTLPLSTAAIVS
                     APVERVEISEPLVTCIMPTHNRRSFVPQAIRCFLRQDYSNLELLVVDDGTEPVGDLVP
                     ANDRIRYVRFDQKLTIGAKRNLANEKARGEFIVHWDDDDWYPSWRVRAQMRALLDRGA
                     DLCGSSRVAYFDPARDQAWEYRYPSAKGPWVAGNTLAYRKSFWSGHKFPDIQVGEDSR
                     FVWSSKGRALADLADPGLCVATVHSTNTSRKNVNAVYWHAQPRDYIDSLIGDEIYFYR
                     TAEASWPLVSCIMPTYERRRYIPQALQSFLQQDYPNRELIIVDDGKDAIGDLVDKLPN
                     VRYFHVARTSIGAKRNLACKHAAGEIIAHWDDDDWYSPDRLRYQVMPIVAGKADLTGL
                     ENAFVLELPRGEFWTTEPALHQRLFVGNVHGGTLVYRKELWSQGLRYPEINLAEDAWL
                     LMRSTRQGKRLVRLSNPGVFVYVRHGHNAWRQFAPGTFIDPKGWQRIATPLHFPVEVL
                     EFYKSASAGR"
BASE COUNT         1285 a         1660 c         1609 g         1208 t
ORIGIN      
        1 tgtcagattc tacgattacg cgagcgaagt tattccgaac gaaaatcccg aggttaaagc
       61 cgaggtcttg attaaagcgg cggacctgtt tgctcgatcg aagacgacca acgatcttaa
      121 taatgcattg gcgcgatact caacagccat caagttgttg gcgagtactg atccacagca
      181 aatcacaatc aatctgcgaa ttggagacgc acaatacgct tcccatcgaa ttcaagaggc
      241 tcgcgaggct taccaaaaag ccgcggagct cgcgcgtgct gcaaagaatt cggcagatta
      301 tggtgaggcg ttacggcgga tcggagatac atacgtgtct ccggaaaagg aaaatgccaa
      361 ggccctcgaa gcatacaacc gcgcgctcgc agcatatgga gtccaggatc ccacttcttc
      421 cggagtgaac ttagtttttg gcgatgcgcg cgttcgcgaa gcgatcgggt tggttaatga
      481 acaaagtgga aacaaaaccc aggcagattc cttttatcgg caggcgaaga agctttacag
      541 tgacctgttg aaacggtcca ccagggattt tgacgagttt ccctcaatga gagttgaaat
      601 ctcttcgcgt ttggatgggg taaccaatag tatcaatcgt ctccaggtta gcccacggta
      661 aactgagtaa cggcaatttg cgcagaatca cgccgttgct tgactcaacg caaaacgctg
      721 ggctagtctg catcagtcac ttacgaccag ccgcttcaca cattggggcc gtagctcagc
      781 tgggagagcg catgactggc agtcatgagg tcaggggttc gatccccctc ggctccacca
      841 atcatttcct tcaatgacga ataagagatc gaaaatggga tcgagcagtc tcatttacga
      901 aatcgaatga agctcattcc cgcctgaaca attcaatttc ccgccaaata aagctggcac
      961 gagctttgac tgtttgcagg cgatcagttc gcattccgcc agacaaaagg atcacttatg
     1021 gacgtgcttt ggaaggacct cgtctactcg gctcgcatgt tgtggaagaa acccagccta
     1081 acggccgtgg cgataatcgc tatcgggctc ggtatcggcg cgaacaccgc gatcttctcc
     1141 gtagtcaaca ctgtcctgct gcaaccgctt ccgtttccac agcccaaaca actcgtcaac
     1201 atcgcgagtg agcaacgcaa tcagacgctc gacgggcgtg gcacttcctc agtgcctgat
     1261 ttcctcgacg tacagaaatc ctcgacgacg ctccagtacg tcgccacgta tcaaggctcc
     1321 ggcacgttca cgaccgatgg caatgagccc gaacgtttac tcggtgcggc ggttagttac
     1381 gactacttca acgtcctcgg cgtcaagccg gagctcggcc gcgtcttcac tcccgacgaa
     1441 gacaagcccg gcgcagcatc ggtcgtgctg atcagtcacg ctctctggca acggcgttac
     1501 gccggtgatc caaacattat cggtcgcgag atcgacctcg gcggcaagac gaccgtcgtg
     1561 ggcgtgatgc cggcgggctt cgagtttccc atcagtgatg acaaccagga ctactgggag
     1621 ccactcttct ccgccgcgtt catgacgaaa gaaatccgcg acgaacgcgc aaatagattt
     1681 ctgccagtca tcggcagact caaacccggc gcaacagtcg cacaggcaaa agcagatctc
     1741 gatctcatct ctcgccaaat cgaacagtcg tcgccagtct caaacacaaa cgtcattttc
     1801 aacaccgtgt cgatgcatga agacgtcaca cgtgactatc gcacggcgct gctcgtcatg
     1861 ctcggcgccg tcggtctcgt cctgctgatc gcgtgcgcaa acgttgccaa cttactactc
     1921 gcccgtgctg ttgcgcgaca gaaagagatc gcgattcgca tggcgctcgg cgcgagtcgt
     1981 cgtcgcatcg cgggccagct cttgatggaa agcgttctgc ttgcattagc cggcggcgcc
     2041 tttggtctgt tgctcgcgac gtggggcacc gatctgctcg tagcctatgg accagccgac
     2101 gtcccgcggc tgcgcgaagt cggcgtcgat cgttacgtgc tcttcttcac gctcggactc
     2161 gcgacgttga ccggcatcgt atttggtctc gcgcccgcgc tgcacgctgc caaaccagcg
     2221 cccggcaaca tgctcaaaga aaacgggcgt ggcgtttaca cgggccgcaa ccggatgcat
     2281 agcgcgttga tcgtgtctga agtggcgttg tcgttgatgc tgctcgcagg cgcaggactg
     2341 ctaatcaata acttctggcg tttactccga accgacgccg gcttcaatcc gaaaggcgtg
     2401 ctcgcgctcg acattcccct cagtagagaa acgtacaaaa caacggagga gcgtgtagcc
     2461 gcatttcaac aactgatcgc gcgaatgaag acggttcccg gtgtgcgcga tgtttcagtg
     2521 gtgagcaacg tgccgttgag tgagaacgac atcgaggtga gctttcaaat cgaaggtcgc
     2581 gagccgtaca aaccaggtga ggaagctgtt gctgattaca cggtcgccgg caacgattac
     2641 ttccgaacaa tggacatcgc gcttctgcga ggacgcgtat tcactgacag cgatacagcg
     2701 aatgcgccgt cagtaatggt cattagtaaa gcattcgcga aggtttactt tccaaacgag
     2761 gatgccatcg gcaaacgcat cattctcgac ggtccaaaca agacgccgcg cgagatcgtc
     2821 ggcgttgtcg acgacgtgcg acgtaaaggg ctcgacagcg acgtgcagcc ggaaatgtat
     2881 ttctcgtacg ttcagagacc ggagcgtcgc ttgaatctcg tgcttcgatc gacgacggaa
     2941 gatgcgtcgc aactgacgcc ggcggcccgc gcggaagtga aggctttcaa tccgcgacag
     3001 atcatctggc gcgcgcaaac actcgaacag atgttgagca cgtcagtcgc tccgaggaaa
     3061 ttcaacatga tgctgctcgg tatttttgcc ggcgtcgcgc tggtgctcgc tgctgttggt
     3121 ctatatggtg tgatgagtta ttcggttagc tggcgcacac aggagatcgg aattcgcatg
     3181 gctctcggcg cgaaacgtac tgatgtgttg cgaatggttg tacgacaggg aatgacgatg
     3241 acgctgatcg gtctcgcgct cgggttggtt ggcgtgatcg cgctgtcgcg cgtgatggtt
     3301 ggactgctct atggcgtgtc gccgacggat ccgctcacgt tcactggcgt gtcgatcgtg
     3361 ttgttgttgg ttgcattgct ggcgtgtttg attccggcac gtcgcgcgac acgcgtcgat
     3421 ccgatcgtgg cgttgagaag tgagtaagag ttatccacag attacgcaga ttgttatctg
     3481 tgttatctgc gtaatctgtg gatgaaactt accttcctgc actggcgctc ttatagaact
     3541 cgagcacctc aaccgggaaa tgcagcggcg tcgcgatccg ctgccagcct ttcggatcga
     3601 taaacgttcc gggcgcgaac tgccgccacg cattgtgtcc gtgcctcacg taaacaaata
     3661 cgcccggatt cgacagtcgc actaatctct tcccctgccg cgtcgatcgc ataagcagcc
     3721 acgcatcctc cgcgagattg atctccggat accgcaaccc ctgcgaccaa agctccttac
     3781 gataaacgag cgtcccacca tgaacatttc ccacgaacaa acgttggtgc agcgccggct
     3841 ccgtggtcca aaactcaccg cgcggcaact cgagcacaaa cgcattctcg agcccggtca
     3901 aatcagcttt gcctgcgacg atcggcatca cctgataacg caatcgatca ggtgagtacc
     3961 agtcgtcgtc atcccagtgc gcgatgatct cgcccgccgc atgtttgcat gcgagattac
     4021 gcttcgcgcc gatcgacgtg cgcgccacat ggaaataccg aacgttcgga agcttatcaa
     4081 ccagatcgcc gatcgcatcc ttgccgtcgt caacgatgat cagctcgcga ttcggataat
     4141 cctgttgtaa aaatgattgc aacgcctgcg ggatgtaacg gcgtcgctcg tacgtcggca
     4201 tgatgcacga cacaagcggc cacgacgctt ccgcagtgcg ataaaaataa atctcgtcgc
     4261 cgatgaggga atcgatgtag tcgcgcggct gcgcgtgcca gtaaacagcg ttcacgttct
     4321 tccggctcgt gttggttgaa tgaacagtcg ccacacacaa tcccggatcg gcgagatctg
     4381 caagcgcacg tcctttgctg gaccaaacaa agcgtgagtc ttcgccaacc tgaatgtcgg
     4441 gaaacttgtg accgctccaa aaactcttgc ggtaggcgag cgtgttgccg gcaacccacg
     4501 gtcccttcgc agacggataa cgatattccc aggcctgatc gcgcgccggg tcgaaatatg
     4561 cgacgcgact actgccacac agatccgcgc cgcgatcgag cagcgctcgc atctgcgcgc
     4621 gcacacgcca cgatgggtac caatcatcat catcccagtg cacgatgaac tcaccgcgcg
     4681 ctttttcatt cgcgagattg cgtttggctc cgatcgtcag cttctgatcg aaacgcacgt
     4741 aacgaatgcg atcgttcgcc ggtacaagat cgccgacggg ctccgtgccg tcatcaacga
     4801 caagcagctc gaggttcgaa taatcctggc gcaggaaaca acgaatcgcc tgcggaacaa
     4861 agctgcgccg gttgtgagtc ggcatgatgc aggttacgag cggctccgaa atctcgacgc
     4921 gctcaaccgg cgccgagacg atcgccgcgg tcgataacgg caacgtgcgc ttgatctcga
     4981 tcagaccgcc cggcgcgaag tttggacatg catcgccgcg acaatgctcg cggtagtctg
     5041 tcttgtcgcc acgcaaacgc gcgccgcaaa acttgtcctg gtagagtttc ttgttctttt
     5101 ggaagcgacg gccttcctcg atccggcgtc gcgcgctgta cggcggacga tgcacaaaag
     5161 ccgagcaggc ccacacgccg cgccagcccg cgcgcgccgc gcgaatgttg tagtccattt
     5221 cccaacacgg accgagcgcg tagctttcat ccgcagcgcc gatcgcctcg agtacttctc
     5281 gtcgcacggc gtaacagaaa tcgccaagac tgtagagcgg ctcgagcgtg cgcacttcgc
     5341 tgccaaaacg ttttgaggct tcgtttgccg cggcgatgat ctgtgcggtg ttacccgagc
     5401 agcgtgggaa agcacactgt tcgttccatg agttgttcgt cgatggaccc gcgagaccgt
     5461 tacgcggatc ggcgtcgagt gcggcgagca aacggtcaag ccaaccggca gcgacttgca
     5521 cgcctgattc gagtagcact aaaagatcgg cgtctgtgag tgaagcgaga cgattgaaac
     5581 aagcggcagt accacgcgcg tctgatgttc cggagagtct caagcgattt gcggcgggga
     5641 gtgcgcgcat ggtggtttcg ttgagcgcga tgccgtcgag taacagcaac agctccacgt
     5701 cgtgaggggc gttggcattg aggctggcga gcgtccagcg caaccgctcg atctcgccgt
     5761 ca
//
LOCUS       DASSFQ010000223         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_843954, whole genome shotgun sequence.
ACCESSION   DASSFQ010000223 DASSFQ010000000
VERSION     DASSFQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843954"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFH96_08070"
     CDS             <1..692
                     /locus_tag="VFH96_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6973966.1"
                     /translation="NISFDIATGQKVALVGRSGAGKSTIISLLLRLYEIEDGAILIDG
                     INVKDYQRESLRQQIAVVMQDSVMFGASVRENIAYGKVDATADEIIRAAKAANAHEFI
                     SKLEDGYDTVIGERGGTLSGGQRQRIAIARAFVRNAPILVLDEPMTGLDVESEAAVRD
                     ALNRLMRGKTCLAITHDLASAATADCVLILENGRMHRTERGSAGSTSKSRLKGEVDPA
                     LPRSVLCVPAS"
     gene            698..1873
                     /locus_tag="VFH96_08075"
     CDS             698..1873
                     /locus_tag="VFH96_08075"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6973967.1"
                     /translation="MNDRPAILFYCQHSLGMGHLVRSLALAERLGQRFRVMLLNGGRL
                     PAGIVVPSNIQFVNLPPLGMDEANQLVSHDKRISVARALDRRKKMIRVTFDNLRPVVV
                     LVELFPFGRKKFANEILPLLQAAREKETRALVVCSLRDILVSRERDREFNDRAATWAN
                     EFFDVVLIHSDPSFARFEESFHSNVPLQVPVKYTGFVVPSGPPTPTESKRKRIVVSAG
                     GGIAGEPLLRTAIEALDYFQDDPSVEMKVIAGPFLSDESWRALRALAQGRNKLRLVRQ
                     VSDLCSELCSASVSISQAGYNTCLDVLRAKVPAILVPFAKEDEDEQRKRALRLQDLGA
                     VRVLDQNELTPARLAAEIRESMTAKFATPALDLNGAENAAALIESITNLADYTNYAH"
     gene            1896..2630
                     /locus_tag="VFH96_08080"
     CDS             1896..2630
                     /locus_tag="VFH96_08080"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HET6973968.1"
                     /translation="MRKTLDEASGSISFFFRDDDAGWEDARLFELLDVFADYDVPIDL
                     AVIPKSISRATAVSLRQRLEENREHVSAHQHGYAHVNHEQTGRKCEFGESRSSDLQRA
                     DIAAGKALLTDLLGPVTESIFTPPWNRCNASTATGLIAEGFTLLSRDITATQFNSPGL
                     GELPVSIDWFGHRKGVRLMPNEIGDSLSKAATSQAPVGVMLHHALIDDERQRIGELLK
                     LLSSHSQVSCRLMRDLVLPFERKAMS"
     gene            2627..3568
                     /locus_tag="VFH96_08085"
     CDS             2627..3568
                     /locus_tag="VFH96_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973969.1"
                     /translation="MRKVLVIIFTIIVTSICVAAQTTDTLKKEDDRLHRRWWVSMSLA
                     SVYDTNITHDEQLVGSFGLVPSFGFHFRDNAEHPSFEADYEVALHRYNHTDEFDRVSH
                     NMTAVYRKQLAKRFYSKTTSEISLKGSSEDREINNNYILEQQLQYRLPFNTRVAGFAA
                     YRLKRYPLIEQDSNSIDSYAGAKVEQRFSGDRRVELTYRYDHNRAWDPRNNYIRRTYS
                     LEFQTPLSIRRRDSLSAEMRYAPRDYQNRTTRVNGIRVPRHDDRWVFSVLYERLLRPD
                     LSMAFEYQYEKRNSNDIDKIFASNVFGLSFTFDNWSW"
BASE COUNT          838 a          997 c         1012 g          774 t
ORIGIN      
        1 gcaacatctc tttcgacatt gcgaccgggc aaaaggtcgc gctcgtcgga cgctcaggcg
       61 cgggcaagtc gaccatcatc agtcttctct tacgactcta cgaaatcgaa gacggcgcga
      121 ttctgatcga cggaatcaac gtcaaagact accaacgcga atcgctgcgc cagcagatcg
      181 ccgtcgtgat gcaggacagc gtgatgtttg gcgcctcagt cagggagaac attgcgtacg
      241 ggaaagtaga cgcaactgcg gacgagatca ttcgcgctgc gaaggcagcg aacgcgcacg
      301 agttcatcag taagctcgag gatgggtacg acactgtgat tggcgaacgg ggaggcacac
      361 tctccggggg gcaacgccaa cgcatcgcga tagcgcgcgc ctttgtgcgg aacgcaccca
      421 tcctcgttct cgatgagccg atgacgggcc tcgacgtcga gagcgaagcg gcagtgcgcg
      481 acgcgctgaa tcgattgatg cgtggcaaga cgtgtttggc tatcacacac gatctcgctt
      541 ccgccgctac agccgactgc gttttgattc tcgaaaacgg tcgaatgcac agaaccgagc
      601 gcggtagcgc cgggtcaact tccaaatcac gactcaaggg cgaggttgac ccggcgctac
      661 cgcgctcggt tctgtgcgta cccgcgagct aaaacaaatg aacgatcgac ctgcaattct
      721 cttttactgt cagcactcgc tgggcatggg ccatctcgtg cggtcgctcg cgctcgcgga
      781 gcggctgggt caacgatttc gcgtgatgct tttgaacggc ggacgactac ctgccggaat
      841 cgtggttccg tcaaacatcc agttcgtaaa tctgccgcca ctcggaatgg acgaagccaa
      901 tcagcttgtc agtcacgaca aacgaatctc ggtagcgcgc gcactcgatc gacgaaagaa
      961 gatgatccgt gtgaccttcg acaaccttcg tccggtagtg gtgctcgtcg agcttttccc
     1021 tttcggccga aagaaatttg cgaacgagat cctgccgcta ctacaggccg cgcgcgaaaa
     1081 agagacgcgc gccctcgtgg tttgcagcct gcgcgacatt cttgtcagtc gcgaacgtga
     1141 tcgggaattc aacgaccgcg ccgcaacctg ggccaacgag ttcttcgatg tagtgttgat
     1201 tcattcagat ccttcattcg ctcgcttcga agaatctttt cactcaaacg ttccgttgca
     1261 ggtgccggtc aagtacaccg gatttgttgt gccttctggc ccacccacgc caacagaaag
     1321 caagcgaaag cggatcgtcg tttcggcagg cggcgggatt gccggcgagc cactactgcg
     1381 aacagcgatc gaagccctcg actacttcca ggacgacccg tcagtcgaga tgaaagtcat
     1441 cgccggcccg tttctgagtg acgaatcttg gcgcgcactc cgcgcgctcg cgcagggcag
     1501 gaacaagttg cgcctcgtgc ggcaggttag tgacctgtgc agtgagttgt gtagcgcgtc
     1561 tgtatcgatc agccaggcgg gatacaacac gtgtctagat gttctgcgcg cgaaggttcc
     1621 ggcgatcctt gttccatttg cgaaagagga cgaagacgag cagcgcaaac gtgcgttgcg
     1681 tttacaggat ctcggcgcag tcagggtgct cgaccaaaac gagctgacac cagcgcgact
     1741 cgccgccgag attcgcgagt cgatgacagc aaaatttgcg actccagcac tcgatctcaa
     1801 cggagcggag aatgcggcgg cgctgattga gtcaataaca aatctcgcgg attacaccaa
     1861 ttatgcacac tgattctaca aactggttgg atccattgag gaaaacactg gacgaggcgt
     1921 ccggatccat atctttcttt ttccgcgatg acgacgcagg ttgggaagat gcgcggctct
     1981 tcgagttgct tgacgtgttt gcagattacg acgtgcctat agatctggca gtgattccga
     2041 aatcgatttc acgagcgacg gctgtgtctc ttcgccagcg tctggaagaa aatcgggagc
     2101 acgtttcagc tcaccagcac ggttacgcgc atgtgaacca tgaacagacc gggcgtaagt
     2161 gtgagtttgg agaaagtcgt tcgagcgatc tgcagcgtgc cgacatcgcc gcaggcaaag
     2221 cactgctgac tgacttgctt gggccagtta cagagtcgat cttcactccg ccgtggaatc
     2281 gctgcaacgc gtccaccgcg acaggcttaa tagcggaagg tttcacactt ctctctcgcg
     2341 acataaccgc aacgcaattc aactcgccgg ggcttggaga gctgccggtg tcgatcgatt
     2401 ggtttggcca tcgcaaaggt gtgcgactga tgccaaacga gataggagat tcgcttagca
     2461 aagccgccac ttcgcaagcg ccggtcggag tgatgctgca tcacgcgctc atcgacgacg
     2521 aacgccaaag gatcggcgag ttgctcaagc tgctttcgtc acattcgcaa gttagctgcc
     2581 ggttgatgcg cgaccttgtt ctaccgttcg aaaggaaagc gatgtcatga gaaaagtctt
     2641 agtcatcatc tttacaatca tcgtcacgag tatctgcgtt gccgcgcaaa caaccgacac
     2701 gctcaaaaaa gaagacgaca ggttacaccg gcgttggtgg gtttcgatgt cgttggcgag
     2761 tgtgtacgac acgaatatca ctcacgacga gcagttagtc ggctcttttg gtctcgtgcc
     2821 gagtttcggg tttcacttca gagacaacgc tgagcacccg tcgtttgagg ctgattatga
     2881 ggttgcgttg caccgctaca atcatacgga tgagtttgat cgagtcagtc acaatatgac
     2941 ggccgtctat cgcaaacaac tcgcaaagcg gttctattcg aaaacgacga gtgaaatttc
     3001 actcaaagga tcgtctgagg atcgtgagat caacaacaac tacattctcg agcagcagct
     3061 tcaatatcgc ctgcctttca acaccagagt cgcgggtttt gcggcgtatc gtttgaaacg
     3121 gtatccgctg attgaacagg attcgaattc gatcgattcg tacgcgggcg cgaaagttga
     3181 gcagcggttt agtggcgatc gacgcgtcga gctcacatat cgttacgatc acaatcgcgc
     3241 gtgggatccg cggaacaatt acattcgccg gacatactcg ctcgaattcc aaactccgct
     3301 ctcgatcaga cgacgcgatt cgttgagcgc tgagatgcgt tacgctccac gtgattacca
     3361 gaaccgaacc acgcgagtta acggcatccg cgtgcctcga cacgacgacc gctgggtctt
     3421 cagtgttctc tacgagcgct tgctccgccc ggacctctcg atggcattcg aataccagta
     3481 cgaaaaacgc aactccaacg acatcgacaa aatcttcgca tcgaacgtat tcggtctgtc
     3541 attcaccttc gacaactgga gttggtagtc agtggtgttt gagtccgcga agcgagcgac
     3601 ggcagcaaag ctcgcgaagc g
//
LOCUS       DASSFQ010000224        20671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1134986, whole genome shotgun sequence.
ACCESSION   DASSFQ010000224 DASSFQ010000000
VERSION     DASSFQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20671
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134986"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFH96_08090"
     CDS             complement(<1..296)
                     /locus_tag="VFH96_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973970.1"
                     /translation="MTPRPEKEPTTRPSDRSRGRVLLIADDRAQFVETLNDRGIDVFG
                     VSNGTAAIVSLTRTRPHLVIADATTRGLRVRELAKMLAQQEDGIQLLLAGPEPST"
     gene            complement(407..1030)
                     /locus_tag="VFH96_08095"
     CDS             complement(407..1030)
                     /locus_tag="VFH96_08095"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET6973971.1"
                     /translation="MTVVKICGITNLDDALAAVAAGADALGFNFYKPSPRYITPQDAR
                     EIVEQLPVTVLTVGVFVNEESEDVRKIAKEVDLRALQLHGDESPEYCRKLADYFVIKT
                     FAVSDTFDIQAAHEYKVEAIMLDTKHNNLRGGTGRVFDWSIAQEAAKTIPKLFLAGGL
                     SPKNVKNAVEIVRPFAVDACSALEDTPGKKNHERMRVFINTVRGVAP"
     gene            complement(1017..1757)
                     /gene="trpC"
                     /locus_tag="VFH96_08100"
     CDS             complement(1017..1757)
                     /gene="trpC"
                     /locus_tag="VFH96_08100"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET6973972.1"
                     /translation="MLSEIIAKKRERVREAKARARPHAFRRALQADGINVIAEFKRRS
                     PSKGMIREGANPVDVAIAYKAGGAVAMSVLTEEDYFAGSLDDLREVKGAVELPVLRKD
                     FIVDEYQVYESAAAGADAILLIVAALDDESLVRLRRLAEDELGMDALVEVHSSDEMRR
                     AAACGAKLIGVNNRDLRTFAVSLETSLSLAREAPSEALLISESGLNSAADLQRLYAVG
                     YRGFLIGETLMRAGDPAAALRSFRNDSR"
     gene            complement(1808..2836)
                     /gene="trpD"
                     /locus_tag="VFH96_08105"
     CDS             complement(1808..2836)
                     /gene="trpD"
                     /locus_tag="VFH96_08105"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET6973973.1"
                     /translation="MPIHPLTEFVARLVRRENLSREEAFDLLEALLDAEATDAQISGA
                     LVALAAKGETVDELTGMAEGLRSRAVRVISKHDCFIDTAGTGSSRAKTFNISTAAAFV
                     IAGAGLPVAKHGNRAASSKSGSADLLTALGVNVSAIPLVSENALNEIGICFMFAPLFH
                     GATARVAGIRKQLGIHTTFNLLGPLSNPAGAPRQIIGVWRKDLGEKLAQVLSGLGTER
                     AWVVHGEDGLDEITLAGKTHVAEAQNGEVRMFEIGPEDFGFAVGAIEHLRGGDAEANA
                     EIVSGVLSGERKDEARALVIMNAAAALFLGGMAADLREGARLAAHAVDSGEARRKLDL
                     LIEATSGP"
     gene            complement(2843..3406)
                     /locus_tag="VFH96_08110"
     CDS             complement(2843..3406)
                     /locus_tag="VFH96_08110"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET6973974.1"
                     /translation="MLLVIDNYDSFTYNLVQYLGELGATVEVRRNDKVTLDEIENRLR
                     PERIVISPGPGTPNDAGITLEVIQRFAGKIPLLGVCLGHQAIGQAFGGKVVRAPELMH
                     GKASDVHHDGKTIFAGLGDQFRAGRYHSLIVERESLPSCLEISAHTADIIMGLRHRDL
                     KVEGVQFHPESILTSDGKQLLANFLKL"
     gene            complement(3480..4130)
                     /locus_tag="VFH96_08115"
     CDS             complement(3480..4130)
                     /locus_tag="VFH96_08115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6973975.1"
                     /translation="MRNFVALIVVSLGLVIAARAQSNNQIEPPKKANARAAATPAPTG
                     DPFDGATVEKMSGQCVTLETELGAIEIAIMPENAPEAARNFLNLAATGAFDTTTFSRV
                     VKGFVIQGGNLQTSEKWSEALAKRMSHKLPDEPGLVKHERGIVSMARTDEPNSATTHF
                     FILVAPAPHLDSKFAAFGTVTKGMEVADAINQAPVEGDKPEKPVKINRAVVSPCKK"
     gene            complement(4174..5682)
                     /locus_tag="VFH96_08120"
     CDS             complement(4174..5682)
                     /locus_tag="VFH96_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET6973976.1"
                     /translation="MLDLQPPSFEAFEREARQGNVVPVVRSVLADLHTPVGAFMRIAG
                     DSPYSFLLESVEGGERIARYSFLGAEPEMIVRGKGSQTIVERDGKTESYLKVATEWVR
                     DYFRGRSLSRRPGLVPFAGGAVGYIGYDAAQWFEPALRDDNTQPLDPPTDAVWMFFRN
                     VIAFDRVKQRLEIVSIVLTEEAGGSHERLRELYDQAVARTKELEDRIFETPAVPRVQT
                     RNDNAESVALQSNWTRRAFEDGVRQVKEHIAAGDCYQVVLSQRFSARFDGDPLQIYRA
                     LRAINPSPYMFFLRLGDESVVGASPEMLVRCHGQRLDYRPIAGTRKRGATETEDWMLG
                     EDLKTDEKEIAEHMMLVDLGRNDLGRVSDYGSVKVDDLMRVERYSHVQHLETSLRSRL
                     RDGLDRFDALASCFPAGTVTGAPKIRAMEIIRELEPDRRGLYAGSVLYMDYADNLDSC
                     IAIRTIVLRDGKAMVQAGAGIVADSVPEHEYVETVNKARAMFRAIEMAERGL"
     gene            complement(5685..6470)
                     /locus_tag="VFH96_08125"
     CDS             complement(5685..6470)
                     /locus_tag="VFH96_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6973977.1"
                     /translation="MTESIFNEGILKGRVAFVTGGGTGITGGVARALAEAGAAVALVS
                     RKMDHLIPAAQAINENGGSAIAVAADVRQPEEVEKAIAQTVEQLGKIDIVVNGAAGNF
                     LCAAEELSPNGFGTVVDIDLKGTFNVCRAAFAQLKEHHGQILNISATLHYLGTPLQLH
                     VSAAKAGVDALTRNLAVEWGRYGIRVNAIAPGPIEDTEGMQRLVPEPVKERLLKKIPV
                     GRFGRIADIEKAAVFLCSDAASFINGVVLVVDGGHWLNGTRLG"
     gene            complement(6477..7202)
                     /gene="hisA"
                     /locus_tag="VFH96_08130"
     CDS             complement(6477..7202)
                     /gene="hisA"
                     /locus_tag="VFH96_08130"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HET6973978.1"
                     /translation="MIVIPAIDLKNGLCVRLLQGRKSEMTVYNEDPVEVARAFAAAGA
                     EMIHVVDLDGAFNEADSPNRAVVRRIIEAVDVPIEFGGGVRSLEDVQQLCDAGVAWVV
                     LGTVAAEAPARLSEFVNSFGSKICVGIDARDGRVMTRGWETATPLMAVDLARSVAECG
                     VERIIYTDIARDGALTGPNINQTLAVARSANVRVTASGGVSSLDDIERLRDAGVDSVI
                     VGKALYEGKFKLEEAIEAADLRG"
     gene            complement(7199..7942)
                     /locus_tag="VFH96_08135"
     CDS             complement(7199..7942)
                     /locus_tag="VFH96_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET6973979.1"
                     /translation="METDELLEFAIDLSRGAGEITLQYFRKKPETSKKSDGSYVTIAD
                     REAESYLRRRIAERFPDDGILGEEEGELEGRSGRRWILDPIDGTFAFVHGVPFYGVLI
                     AVELKGEMSVGVVNIPALGEIVSAAKGRGCFLNGEPARVSTTVELKDALLLSTSFSRA
                     TESLQARAKVSRTWGDCYGYVLVATGRADVMLDPVMNLWDCAPLLPIMEEAGGTFTDW
                     RGVRTANGGNSIATNGALFEEVMSLIRQQ"
     gene            complement(7932..8555)
                     /gene="hisIE"
                     /locus_tag="VFH96_08140"
     CDS             complement(7932..8555)
                     /gene="hisIE"
                     /locus_tag="VFH96_08140"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HET6973980.1"
                     /translation="MINFDEIRFDERGLIPAIVQDAATREVLTLAYMNRESLARTLET
                     RQTWFWSRSRNELWHKGETSGNTQEVVSLALDCDADAIIVLVKPAGPACHTGAVSCFD
                     TGAQPQSLGLLLDQLYELIQSRERERPSGSYTTYLFEAGLDKILKKVGEESAETIIAA
                     KNEDEARLAEETADLLYHLIVLLVARGVSLTDVAQELAQRKKGQSGN"
     gene            complement(8552..9307)
                     /gene="hisF"
                     /locus_tag="VFH96_08145"
     CDS             complement(8552..9307)
                     /gene="hisF"
                     /locus_tag="VFH96_08145"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET6973981.1"
                     /translation="MLAKRIIPCLDVDNGRVVKGIRFVSLTDAGDPVAQAKRYDLEGA
                     DELTFLDITASSERRDIVAELVRRVADEVFIPFTVGGGLRSVEDIRAILRAGADKITL
                     NTAAVDDPELIKACAETFGSQCVVVAIDARRREQGGWEVYTHGGRRPVGLDAIEWAMR
                     AEQLGAGEILLTSMDCDGTRNGYDIELTRAVSDAVRIPVIASGGAGKVEHFYDALTAG
                     GASAVLAASLFHFGELSIADVKSYLRSRGLEVR"
     gene            complement(9310..9909)
                     /gene="hisH"
                     /locus_tag="VFH96_08150"
     CDS             complement(9310..9909)
                     /gene="hisH"
                     /locus_tag="VFH96_08150"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET6973982.1"
                     /translation="MSVAIIDYGVGNLRSVEKAFAATGCEAIVSGDESVLREAERLVL
                     PGVGAFGACMKALKERGFDGLVKERVREGTPLLGVCVGMQLLFDESEEFGSTQGLGLL
                     RGSVRRFSGDLVVPHIGWNRIHQKRSHELFEEIEEGSFCYFVHSFYCEPVDESVVVGE
                     TEYGVRYASVVADGNVCGVQFHPEKSQDVGLRMLRNFCG"
     gene            complement(9906..10496)
                     /gene="hisB"
                     /locus_tag="VFH96_08155"
     CDS             complement(9906..10496)
                     /gene="hisB"
                     /locus_tag="VFH96_08155"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET6973983.1"
                     /translation="MQSRIANVRRKTKETDVRVELNLDGSGQSSVSIGLPFLEHMLEL
                     FAKHGLFDLQVTCKGDLEIDDHHSVEDIAITLGQALVQALGDKSGINRYGEAVVPMDE
                     ALCRSVIDLSGRFYLVYEVPTRRQMIGNFSVELAEHFWRSFAETAKLNLHIDCLRGRN
                     THHILEGTFKASARALRQAVERNSRILGVPSTKGSL"
     gene            complement(10507..11577)
                     /gene="hisC"
                     /locus_tag="VFH96_08160"
     CDS             complement(10507..11577)
                     /gene="hisC"
                     /locus_tag="VFH96_08160"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET6973984.1"
                     /translation="MKEALDSIKPSVRAVKAYTLPAHRASLKLNQNENPWDAPAHIKE
                     EVLRRFAARKWSQYPDFVPAQLNECLANFAGWRSDGIIAGNGSNELIQALLMVTMETG
                     KTLLLSEPTFTLYRQVATVLGGRVETVLLKSNLEYDSEALQRFVEEKQPDVTIICSPN
                     NPTGCAIDDAALSSLLKTSRGLVVIDEAYHEFAGHTVVPLLKEYENLVVLRTFSKAMA
                     VAALRIGYLLASPDLVREIGKAVLPYNVNVFSQVAAEVAVENYESELRPLVREIVSER
                     DRLFAALSEIDGLAPVASHANFILVKSATDPKRMFTELLARDILIRDVSGYPMLSEYF
                     RFSVGTPEENDYVLKAIREICG"
     gene            complement(11574..12875)
                     /gene="hisD"
                     /locus_tag="VFH96_08165"
     CDS             complement(11574..12875)
                     /gene="hisD"
                     /locus_tag="VFH96_08165"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET6973985.1"
                     /translation="MICRINKMELVERLAARSVAFDADLMKVVGAIVDDVRVRGDDAL
                     IDYTARFDKVELRQLRVSDEELRRCAAGVDECVQRALREAIANVREFHERQVEESWTI
                     NPRAGVHLGQRITPLERVGLYVPGGTAAYPSSVVMNVVPAQVAGVERIVVTTPPRTLS
                     ESSAVAAALVELNVNEVYAVGGAQAIAALAFGTQTVPRVDKITGPGNKYVAAAKKIVF
                     GVVGIDAIAGPTEVVIVADKTARADFIAADLLAQAEHGEDASAVLITNSESLARDVAE
                     EVARQAESLPRREIVQASLRDYGAIVLVETLDEACALVNELAPEHVEIVTSDDEAIGA
                     MIRHAGAIFFGSYTPEAVGDYLAGPNHVLPTSRTARFSSALGVYDFVKRTSLLRYSKD
                     AFAAAAESVAVLAECEGLSGHARSALIRKDRHEKAQKAQNL"
     gene            complement(12888..13508)
                     /gene="hisG"
                     /locus_tag="VFH96_08170"
     CDS             complement(12888..13508)
                     /gene="hisG"
                     /locus_tag="VFH96_08170"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF013773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET6973986.1"
                     /translation="MITIAIGKGRMQESALALLARAGIRLNDGVNSRRLALTDETLSY
                     RFIFVKPADVPVYVEHGIADCGIVGRDVLLESEADLLLPLDLGISRCRIVVAAADPEA
                     MTNVQGMIRVATKYAQIARAHFGARGIPVEIIQLSGSVELAPALGLADMIVDLVETGV
                     TLRENGLTVVEEIAESTGRLVVNRASYQLKSDEVGRLIKVLEDVVS"
     gene            complement(13486..14484)
                     /gene="hisZ"
                     /locus_tag="VFH96_08175"
     CDS             complement(13486..14484)
                     /gene="hisZ"
                     /locus_tag="VFH96_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET6973987.1"
                     /translation="MTSRIPAGMRYYIGAEARLRRTVEDVAMSVFQAWNYEEVITPSV
                     DYYDLFEQGMGQREAQRGFRFTDNDGRLLALRPDVTSSVARMAATLLSERPRPLRFCY
                     AAPVFRQQTQSHAEWRRENTQLGGELIGTGGKPADLEVLRLAAEILARLNLQSRYCIT
                     INNVEIFNGVAAHLNLEERAREQLRRLIDTRETAELQRFLKNYDNPEARAFSQPAQLT
                     GKRNVIQAARHAITNARSLAALDSLEALWTEIESLGFESSFEIDLGDVSSLDYYTGLS
                     LKIFVHGAGASVGRGGRYDGLTGSFGRAEPAVGFVLNLDALTEVLGRNKDDNDRNR"
     gene            complement(14558..14695)
                     /locus_tag="VFH96_08180"
     CDS             complement(14558..14695)
                     /locus_tag="VFH96_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6973988.1"
                     /translation="MTSIGRVRYKPKDMFTTDRGHHANWHHHHTGGGLAIVFAALTEA
                     S"
     gene            complement(14779..16668)
                     /locus_tag="VFH96_08185"
     CDS             complement(14779..16668)
                     /locus_tag="VFH96_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET6973989.1"
                     /translation="MTQTSKKVFFVLFVLFVAKTAVLAQKSLPAIRSQADFDKISVTY
                     DANTPYALPHVMIVIDRKDGNKIYYVNKKRYSFHKDFVNGTYLSLERGREFFVNNYIK
                     PNRRFILGTLAYQTPIKRWVFEFWEGDLIPADQIQLAYDVINKTFFAPVAFKPNSLRQ
                     EEATRSLAGMQRVLQSDIAKEQAYQALNMAKGLGRIHIIPKLDDHVEIGFNEILVLDE
                     VPVQLPPVAGIITSQPSTPLSHINLLAKGWNIPNAYIKNAKELLKQYDGWWVSFETLR
                     ENYTIRRADMNQLREYQRRQVERLDVMKPRYNLDETRLLSLAEQRSKSSLAFGGKSAN
                     LGEVLNARLPGIVVPSGFTIPFYYYDDFIKRNNLDDTIYGLLNDQKFVHDPAYRREQL
                     VQLRQKIESAEFDPKLKEMVLQKVAREYPDKGLFVRSSSNSEDLPNFSGAGLYTTVPN
                     VRGEQPLIDAIKKVWASLWNFEAYEARERASVDHSKIFMAVLLQEGINSESSGVMIST
                     DPFDNENKGAIYISAKRGLGIKVVEGQRIAEQIIFRPRTNAIQVLTRSAEDSLLTFDE
                     NGGVKEVPIEGDRVVLTDEVIRRLVRAATAIKRVFGARDQDIEWAYMKGQIYIVQSRP
                     FIPGG"
     gene            complement(16670..18115)
                     /locus_tag="VFH96_08190"
     CDS             complement(16670..18115)
                     /locus_tag="VFH96_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6973990.1"
                     /translation="MIEDFVEQHRFLGPAVAQIITDHVAGLATRRVTPESTPAELEKL
                     FDEPLPEKGIELEEILARFKNEIAPNAMGVPSPRYFGQFNPTPLPIGVWADALSSMLN
                     QNAGAWRNGPTSAMIEARVMRWLCDLLDYGPQSFGTLASGGSEANLIALKCARDSVAA
                     SIKDRGVRTAPGDLVIYASEQCHYSIDKSADILGLGREGVRKIPTDDRFHIIPDALRE
                     TVAKDREAGLIPCCIVGVAGTTSTGVIDPLEELAEIARANRCWYHVDAAYGGPLAFSP
                     KHKDRLRGINLADSITFDPHKWMFVPFSCGATLVRDGGRVLRDAFDMTPEYLSEDRGG
                     TDVEFDFFRYGQMGTRRFNSLKLWMALKFMGREGYARTVEHQIDMTRYLAQKIDELEN
                     FTRVGEVETAVCCFRLNGAETDRLQQRLQQIIERSGEAWLTTTVLHGRRAMRVNVNSF
                     LTEKHHIDGLIELLVKSAEEITKGTKETKGT"
     gene            18133..18735
                     /gene="rsmG"
                     /locus_tag="VFH96_08195"
     CDS             18133..18735
                     /gene="rsmG"
                     /locus_tag="VFH96_08195"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET6973991.1"
                     /translation="MNTFEQSLLSNMESFDLDLSPETVTQLGEYYSLLTRWNDRLHLV
                     APCSAEEFAVRHVLESLLLLRFLPANARVADIGSGGGLPIVPCLIARPNLEATLIESS
                     QKKVVFLREASNRLGIRTSIIARPFEDVPAPRVSFVTCRALDQFMGKLPALINWAPRG
                     STLLLFGGETLGEQLRRASVNFEQLLIPQSEKRYLFLATD"
     gene            complement(18788..19774)
                     /gene="ligD"
                     /locus_tag="VFH96_08200"
     CDS             complement(18788..19774)
                     /gene="ligD"
                     /locus_tag="VFH96_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET6973992.1"
                     /translation="MNSKQIKNLPGARKTSMPEFVPPQLATLTAKPPSGEEWFHELKF
                     DGYRLLCHIEGRQVRFWTRNRKDWTLKFASVGKAVKALPLKSAILDGEIVALDPSGRA
                     SFQRLQQSINKGAAAGFVYNVFDLIYIEGFNITKTPLRERKRLLAELLEPLGDTGTLR
                     YSDHIEGNGAQFFKEACKLGLEGIVSKLADSAYESTRSRNWMKIKCIKRQEFVIAGYT
                     LSDKGIPFSSLILGFYEKGKLIYAGRAGTGYTNTMRVELKKKLDRIAVDKRPFLEIPK
                     DPGLRHAVWTEPKLVGEVAFTEWTDEGVIRHPSFQGLREDKKPTDVVKEEPS"
     gene            complement(20321..20671)
                     /locus_tag="VFH96_08205"
                     /pseudo
     CDS             complement(20321..20671)
                     /locus_tag="VFH96_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4645 a         6581 c         5007 g         4438 t
ORIGIN      
        1 gttgaaggtt caggaccggc caggagaagc tgaattccat cttcctgctg tgcgagcatc
       61 ttcgcgagct ccctgactcg taaaccccga gtcgtcgcat cagcgatgac caggtgtggc
      121 cgcgtccgcg tgagcgagac aatggcggca gtgccgttcg aaacaccaaa gacatcgata
      181 ccgcggtcgt tgagagtctc gacaaattgc gcccgatcgt ccgcgatcaa aagcacgcga
      241 ccgcgcgaac ggtcggaagg tcgggtagtt ggttcttttt cgggcctcgg ggtcatatcc
      301 agacgtaggg aggtcgacaa atcaaataaa gttgttcggg gaggaccttc tgaaatttct
      361 atgtttaggg gcaagagtta ggatactccg cgactgtagg gaaacgtcaa ggcgcgacgc
      421 cccgaaccgt gtttataaat acacgcatgc gctcgtggtt cttttttccg ggcgtgtctt
      481 cgagcgcgct gcaggcgtcg acggcgaacg ggcgcacaat ttccactgca tttttgacgt
      541 ttttgggcga caggccgccc gcgagaaaga gtttcgggat cgttttggct gcctcttgcg
      601 caatcgacca gtcaaacacg cggccggtgc caccgcggag attgttgtgt ttcgtatcga
      661 gcatgatggc ttcgacttta tactcgtgcg ccgcttgaat atcgaaagtg tctgacacgg
      721 cgaacgtctt gatgacgaaa tagtcggcta gtttgcggca gtattcgggc gactcgtcac
      781 cgtgtagttg cagtgctcgc agatcgactt ctttggcgat ctttcttacg tcctctgatt
      841 cctcattaac aaacacaccg accgtcagca ccgtcaccgg aagctgttcg acgatctcgc
      901 gtgcatcttg cggcgtgatg tagcgcgggc tcggtttgta gaaattgaac ccgagcgcat
      961 ccgcaccagc agctacggca gcgagcgcgt cgtcgagatt cgtaatgccg cagatcttaa
     1021 cgactgtcat ttctgaaact acggagtgcc gctgccggat cgccagcgcg catcaacgtc
     1081 tccccaatca aaaacccgcg ataaccgact gcatacaaac gttgcaaatc agcggcgcta
     1141 ttcagtccac tttcgctaat caacaaagct tcggacggcg cctcgcgcgc gagtgaaagc
     1201 gacgtctcga gcgacactgc aaacgtacgc agatcacgat tattgacccc gatcagcttc
     1261 gccccgcacg ctgcggctct tctcatctcg tcactcgaat gaacctccac aagcgcatcc
     1321 attcccaatt catcctcagc aagccgtcgc aaccgtacca acgattcatc atcaagcgca
     1381 gcgacaatca ggagtatcgc atcagcccca gcagcggcag actcataaac ctgatactcg
     1441 tcgacaataa aatctttgcg cagcaccggc agctccaccg cgcccttcac ctccctgaga
     1501 tcatcaagcg aacccgcaaa ataatcctct tccgtcaaca ccgacatcgc aaccgcaccg
     1561 cctgctttat atgcaatcgc aacatcaacc gggttcgcgc cctcccgaat catccccttc
     1621 gacggcgatc gcctcttaaa ttccgcgata acattaatgc catccgcttg caaagccctg
     1681 cggaaggcgt gcggacgcgc gcgagccttc gcctcacgca cccgctctcg cttcttcgcg
     1741 attatttcag acagcacatc catctaaaat ccaaatccgc gttttgaaaa tccgcgttca
     1801 tccgcgttca gggtccactc gtcgcctcga tcaacaaatc caacttgcgc ctcgcctctc
     1861 cactatcaac cgcatgcgcc gccagcctcg ccccttcacg cagatccgcc gccatcccac
     1921 caaggaacaa cgcggccgcc gcattcatga tcaccaacgc ccttgcttca tctttccgct
     1981 cgcccgagag aaccccactc acgatctcgg cattcgcctc cgcatcacca ccacgcaaat
     2041 gttcgatcgc cccgacagca aacccaaaat cctccggccc gatctcaaac atcctcactt
     2101 ccccgttctg cgcctcagcc acatgcgtct tacccgccag cgtgatctca tccaacccat
     2161 cttcaccatg gaccacccac gcgcgctcag tcccgagccc cgacaacacc tgcgccagct
     2221 tctcgcctaa atccttgcgc cacacaccga tgatctgccg cggcgcgccc gccggattgc
     2281 tcaacggccc gagcagattg aacgtcgtat gaataccgag ttgctttcga atacccgcca
     2341 ctcgcgccgt cgcaccatga aagagcggcg caaacatgaa acagatccca atctcgttaa
     2401 gcgcgttctc ggaaaccaac ggtatcgccg aaacattcac accgagcgcc gtcaacagat
     2461 ccgcactgcc tgacttacta ctcgccgcgc gattcccgtg cttcgccacg ggcagaccag
     2521 cgcccgcgat cacaaatgcc gccgcagtcg agatgttgaa tgtcttcgca cgactcgatc
     2581 ccgtccccgc cgtatcgata aaacagtcat gcttcgaaat cacacgcacc gcgcgcgagc
     2641 ggagaccttc agccattccg gtcaactcat cgaccgtctc acccttcgcc gcgagcgcga
     2701 ctagcgcccc cgagatctgc gcgtcagtcg cttctgcatc gagcaacgcc tcaagcagat
     2761 cgaaagcttc ttcccggctc aggttttcac ggcgtacgag ccgcgcgacg aattcagtta
     2821 aaggatgaat gggcacggta ggtcagagct tcaggaaatt tgcgagcagt tgtttaccat
     2881 ctgaggtcag gatcgattcg ggatgaaact gaacgccctc cactttcaaa tcacgatgac
     2941 gcaatcccat gatgatgtct gcggtatgag ccgagatctc cagacacgat ggcaatgatt
     3001 ccctctcaac gatcagcgag tgataacggc ccgcgcgaaa ctgatcgccc aatccggcga
     3061 agatcgtctt gccgtcgtga tgaacatccg acgctttacc gtgcatcaac tcaggcgcac
     3121 gcacgacttt cccgccaaac gcctgaccga tcgcctggtg gcccagacac acaccaagca
     3181 gcggtatctt tcccgcgaat cgctggatca cctcgagcgt gatcccggca tcgttcggag
     3241 ttcctggccc aggcgagatc acgatccgct ccggacgcag tcgattctca atctcgtcga
     3301 gcgtgacttt atcgttgcga cgcacttcca ccgtcgcccc cagctcaccg agatattgca
     3361 cgaggttgta agtgaacgaa tcgtagttgt cgattactaa cagcattgtc tttttagcca
     3421 cggatgaaca ccgatgacac agataagaaa aaccgtgttc atctgtgaaa atccgtggct
     3481 tacttcttac acggactaac cacagctcga ttgatcttca ccggcttctc cggcttatcg
     3541 ccttcaactg gcgcctggtt gatcgcgtca gccacttcca tccccttcgt caccgttccg
     3601 aacgcagcaa atttgctgtc gagatgcggt gccggcgcga ccagaatgaa gaaatgcgtc
     3661 gtcgcgctat tcggctcatc ggtacgagcc atcgatacga tgccacgctc atgtttcact
     3721 aaaccaggct catcaggaag cttgtgactc attctcttcg cgagcgcctc gctccacttc
     3781 tcgctcgtct gcaaattccc accctgaatc acgaacccct tgacgacgcg actaaacgta
     3841 gtcgtatcaa acgcacctgt ggccgcgaga ttcaaaaaat ttcgcgctgc ttccggagca
     3901 ttctcaggca tgatcgcgat ctcgatcgca cccagctcag tctcgagcgt cacacactgc
     3961 cccgacatct tctcaaccgt cgcgccatca aacggatcac cggtgggcgc cggcgtcgca
     4021 gccgcgcgag cattagcttt tttgggcggc tcgatctgat tattcgactg cgccctcgcc
     4081 gctataacca accctaaact caccacaata agtgcaacaa aattcctcat aaaaatccgc
     4141 gtaaatcgtc ttagaaatcc gtcagatccg tgttcataat ccgcgttccg ccatttcaat
     4201 cgcacgaaac atcgcccgcg ccttattcac tgtctccaca tactcatgct ccggtaccga
     4261 atcagccacg atccccgcgc ccgcctggac catcgccttc ccgtcacgca acacgatcgt
     4321 cctgatcgca atacacgaat cgagattgtc cgcataatcc atgtacaaca ccgaacccgc
     4381 ataaagcccg cgacgatccg gctcgagctc gcgaatgatc tccatcgcgc gaatcttcgg
     4441 tgcacccgtc acagtccccg ccggaaaaca cgatgccagc gcgtcaaacc gatcgagccc
     4501 gtctcgcagt ctcgatctca aactcgtctc cagatgctgt acgtgcgaat aacgttcgac
     4561 gcgcatcaga tcatcaacct tcaccgagcc gtaatccgac acgcgcccga gatcgttgcg
     4621 cccgagatcg acgagcatca tgtgctccgc gatctctttc tcatccgttt tcaaatcttc
     4681 gccgagcatc caatcttccg tctcagtcgc gccacgtttc ctcgtgcctg cgatgggacg
     4741 atagtcgagt cgctgtccat gacaacgcac gagcatctcc ggcgatgcgc ccacgacact
     4801 ctcatcaccg agccgcagga aaaacatgta cggcgacgga ttgatcgcgc gcagggctcg
     4861 atagatctgc aacggatcgc catcgaaccg cgccgagaac cgctgcgaca gcacgacctg
     4921 ataacagtcg ccggccgcga tgtgttcctt cacctggcgc acgccatctt cgaacgcgcg
     4981 gcgcgtccag tttgattgca aggcgacgct ttcagcgttg tcgttgcgcg tttgcacacg
     5041 cggcacagca ggcgtttcaa agatccgatc ttcgagttct tttgtgcggg cgacggcctg
     5101 atcgtaaagc tcgcgcagtc gctcgtgact accgccggcc tcttcagtaa gaactatcga
     5161 aacgatctcg agtcgctgct tcacgcgatc gaacgcgatc acgtttcgaa aaaacatcca
     5221 gactgcatcc gtcggaggat cgagcggctg cgtgttgtcg tcgcgcaacg cgggctcaaa
     5281 ccactgagcc gcgtcgtaac cgatgtaacc gactgcgccg ccggcaaacg gcacgagacc
     5341 cggccggcgc gagagagatc gtccgcggaa ataatctctg acccactcag tcgcgacctt
     5401 cagatatgac tcggtcttgc cgtcgcgctc aacgatcgtc tgcgatccct tgccgcgcac
     5461 gatcatctcc ggctcggcgc cgaggaacga gtagcgcgcg attcgctcgc caccttcaac
     5521 cgattcgagc aggaatgagt acggcgagtc accggcgatg cgcatgaacg cgccgaccgg
     5581 cgtgtgcaaa tccgccagca ccgagcgcac cacgggcaca acattaccct gccgcgcctc
     5641 gcgctcaaac gcctcgaacg atggtggttg cagatcgagc ataactatcc gagccgcgtg
     5701 ccgttgagcc agtgtcctcc atcgacaacc agcacgactc cattaataaa actcgccgca
     5761 tcagaacaga gaaataccgc ggccttttca atatcagcga ttctcccaaa acgtccgacc
     5821 ggaatcttct ttagtagacg ctccttcacc ggttccggca cgagacgctg catgccttcc
     5881 gtgtcttcga ttggaccagg cgcgatcgcg ttcacgcgaa tgccatagcg tccccactcc
     5941 accgcgagat tgcgcgtcaa cgcatcgaca cccgctttcg ccgcagacac gtgaagctgc
     6001 agcggcgtcc cgaggtaatg aagcgtcgcg ctgatgttca gtatctgccc gtgatgttcc
     6061 ttgagttgcg caaaagccgc gcggcagacg ttgaacgtcc cctttagatc gatatcaacc
     6121 accgttccga atccattggg cgacaactcc tccgcggcac aaagaaaatt tcccgccgcg
     6181 ccatttacaa cgatgtcgat cttgccgagc tgctcgaccg tttgcgcgat cgccttctca
     6241 acttcttccg gctgcctaac atcagcagcc acggcaatcg cgctcccacc gttctcattg
     6301 atcgcctgcg cggcaggtat aaggtgatcc atcttgcgac tgacgagcgc tacagcagcg
     6361 cccgcttcgg caagcgcacg cgcaacacca cccgtgatcc cggtcccacc accggtgacg
     6421 aacgccacgc gccccttcaa tattccttca ttaaagatgc tttcagtcat ccgcgtttat
     6481 ccgcgtaaat ccgcggcctc aatagcctct tccaatttaa acttcccctc gtataaagcc
     6541 ttgccgacaa taacactatc tacacctgca tcgcgcaagc gttcaatatc atcaagactc
     6601 gaaacgccac ccgacgctgt cacccttaca ttcgccgatc gcgcaaccgc aagtgtctga
     6661 ttgatgtttg gacccgtgag agcaccatca cgagcgatgt cagtatagat aatcctttcg
     6721 acaccacact ccgcaaccga ccgcgccaga tcaacagcca tcagcggcgt cgccgtttcc
     6781 cagccacgcg tcatcacgcg gccatcgcgc gcgtcgatcc caacacaaat cttagatcca
     6841 aagctattca cgaattcact caaccttgcc ggcgcttccg ccgccactgt tccgagtacg
     6901 acccacgcga caccggcatc gcaaagctgc tgcacatctt cgagcgaacg aaccccacca
     6961 ccaaattcaa tcggaacatc cactgcctca atgattcttc tgactaccgc ccgattaggt
     7021 gaatcagctt cattgaatgc tccatcgaga tcaaccacat gaatcatctc tgcacctgca
     7081 gcagcaaacg cgcgcgccac ttcgaccgga tcctcattgt aaacagtcat ctcagacttt
     7141 cgcccctgca acagccgcac acacaaacca ttcttgagat cgattgccgg tataacgatc
     7201 attgttgacg aatcagactc atcacttctt caaacaacgc gccattcgtc gcgattgagt
     7261 taccaccgtt cgctgtccgc acgccgcgcc aatcagtaaa cgttccgcca gcctcttcca
     7321 tgatcggcaa cagcggcgca caatcccaca ggttcatcac cggatccagc atcacatcag
     7381 cccggccagt cgcgaccaga acataaccat agcagtcgcc ccacgtccgg ctcaccttcg
     7441 cacgcgcttg cagcgactca gtcgcgcgac taaagctggt tgacaacaac agcgcatcct
     7501 tgagctcaac cgtagtcgat acccgcgccg gctcaccatt caaaaaacat ccgcgcccct
     7561 tcgcagccga aacgatctcg ccaagcgccg gaatattcac gaccccgaca ctcatctcgc
     7621 ctttgagctc gaccgcgatc aaaaccccgt aaaacggaac gccatgaaca aaagcaaacg
     7681 tgccgtcgat cggatcgagt atccagcgac gccctgaccg accctccaac tcaccttctt
     7741 cttcacccag aattccgtcg tcaggaaaac gttcggcaat ccgcctgcgc aggtaactct
     7801 cagcctctcg atccgcgatc gtgacgtaac tgccgtcaga cttcttgctc gtctccggct
     7861 ttttgcgaaa gtactgcaac gtgatctcgc cggcgccgcg agagagatct attgcaaact
     7921 cgagcaactc gtcagtttcc actttgcccc ttttttcgtt gcgcgagctc ctgtgcgacg
     7981 tctgtcagac tgacgccacg cgccaccagc aacacgatca ggtgatacaa caggtccgcc
     8041 gtttcctcag ctaaccgcgc ctcgtcttca tttttggcgg cgatgatcgt ctccgcagat
     8101 tcctcgccga ctttcttcag tatcttatcg aggcctgctt cgaagagata agtcgtgtaa
     8161 gaaccggaag gccgctcacg ctcacgactc tggatcaact cgtacagttg gtccaataag
     8221 agtccgagag actgcggttg cgctccagta tcaaagcacg aaaccgcgcc cgtgtgacac
     8281 gccggcccgg caggcttgac cagcacaatg atcgcgtccg catcgcagtc aagcgcgaga
     8341 ctcacgacct cctgcgtgtt gcccgacgtc tcgcccttgt gccagagctc gtttcgcgat
     8401 cgcgaccaga accacgtctg cctggtttcg agcgttcgcg cgaggctctc gcgattcatg
     8461 taagcaagcg tgagcacttc tcgggttgca gcgtcctgca cgatggcagg tatcaggccg
     8521 cgctcgtcga aacgaatctc gtcgaagtta atcatcttac ttccagccca cggcttctta
     8581 agtaactctt cacgtcagca atcgacaatt cgccgaagtg aaaaagtgac gcggcgagga
     8641 cggcactcgc gccgcctgct gtcagcgcgt cgtagaaatg ttcgaccttg ccggcgccgc
     8701 cggaagcgat caccgggatt cgaacggcgt cggacacagc acgcgtcaac tcgatatcgt
     8761 aaccgttgcg cgtgccgtcg caatccatac tcgtcagaag tatctcaccg gcgccgagct
     8821 gctctgcgcg catcgcccat tcgatcgcat caagaccaac agggcgtcga ccgccatgcg
     8881 tgtaaacttc ccaaccaccc tgttcacgcc tgcgcgcatc gattgctacg acgacgcact
     8941 ggcttccgaa agtctccgca caggctttga tcaattccgg atcgtcgacg gccgcggtgt
     9001 tcagcgtgat cttgtcggcc ccggcgcgca gtatcgcgcg aatgtcttcg accgatcgca
     9061 aaccaccacc gaccgtgaac ggaataaata cctcgtcggc aacacgacgc acgagctcgg
     9121 caacgatatc gcggcgctcg gaagacgcgg tgatgtcgag aaacgtcaac tcatcagcgc
     9181 cctccaaatc gtaacgcttg gcctgcgcaa ccggatcacc cgcatccgtg agcgagacaa
     9241 agcgaatgcc tttcacgaca cggccgttgt caacgtcgag acagggaatg attcgtttag
     9301 ccagcatctt tagccacaga aatttctcag catccgtagc ccaacgtcct ggctcttctc
     9361 cggatgaaac tgcacaccac aaacattccc atccgcgacc actgacgcat agcgcacacc
     9421 atactcagtc tcacccacaa ccacgctttc atccaccggc tcgcagtaaa acgaatgcac
     9481 gaaataacag aacgatccct cttcaatctc ttcaaacaac tcatgcgatc gcttctggtg
     9541 aatccggttc catccaatat gcggcacaac caaatcgcca ctaaacctcc gcacgcttcc
     9601 tctcaataat cccaagcctt gcgtcgatcc aaactcctca gactcatcaa acagcaactg
     9661 cattccaacg cacacaccca gcaacggtgt cccttctctc actctttcct tcacgagccc
     9721 gtcgaagcca cgttctttca gcgccttcat acacgcccca aacgcaccaa cacccggcaa
     9781 taccagccgc tcggcttcac gcaacacact ctcatcacca ctgacgatcg cttcacaccc
     9841 tgtcgccgca aaggccttct cgaccgagcg caagttaccg acgccgtagt cgatgatcgc
     9901 tacactcaca acgatccttt cgtgctcgga actccgagga tgcgactgtt tcgctcgacc
     9961 gcctgtcgca acgcgcgcgc agaagctttg aacgtgcctt cgagaatgtg gtgtgtgttg
    10021 cggccgcgga ggcaatcgat atgaagattg agcttcgccg tctccgcaaa cgatcgccag
    10081 aaatgctccg cgagctcgac cgaaaagttt ccgatcatct gtcgccgcgt cggcacctcg
    10141 taaacaagat agaacctgcc cgacaaatcg atcaccgagc gacacagcgc ctcatccatc
    10201 ggcacgacag cttcaccata gcgattgatt ccggacttat ctccgagtgc ttggaccaac
    10261 gcctggccca acgtgatcgc aatgtcctcg accgaatgat gatcgtcgat ctcgagatcg
    10321 cctttgcacg tcacctgcaa gtcgaataat ccatgcttgg cgaagagctc gagcatatgt
    10381 tcgagaaacg gcaaaccgat cgagacactc gactgtccgc tgccgtcgag attgagctca
    10441 acacgcacgt cggtttcttt cgttttccgc cggacgtttg cgattcgtga ttgcatggtc
    10501 gtttgtttag ccacagattt cacggattgc tttcaacacg taatcattct cttcaggcgt
    10561 gccgacgcta aatcgaaaat actcgctcaa catcggatag ccactcacgt cgcgaatgag
    10621 aatgtcgcgc gcgagcagct cggtaaacat ccgtttcgga tcggttgccg acttcaccag
    10681 tataaaattc gcgtgcgaag ccaccggcgc gagaccgtcg atctcagaca acgccgcaaa
    10741 cagacggtcg cgctccgaga cgatctcgcg aacgagtggt ctaagctcgc tttcgtagtt
    10801 ttcaacggcg acttcggcag cgacttgcga gaacacgtta acgttgtaag gcagcacggc
    10861 tttgccgatc tcgcgcacga ggtcaggcga tgcgagcagg taaccgatgc gcaacgcagc
    10921 gacagccatc gcttttgaga aggtgcgcaa cacgaccagg ttctcgtact ctttgagtaa
    10981 cgggacgact gtgtggccgg caaactcgtg atacgcctcg tcaataacga ccaggccgcg
    11041 cgaggttttg agtagagagc tcaacgcagc gtcgtcaatc gcgcaaccgg tcggattgtt
    11101 cggcgagcag atgatcgtca cgtccggctg tttctcttca acgaatcgct ggagcgcttc
    11161 gctgtcgtat tccaaattag acttcagaag aaccgtttca actctaccgc caagcaccgt
    11221 tgcgacttgc ctgtacagcg tgaaggtagg ctcagaaagc agaagcgtct ttccagtctc
    11281 catcgtgacc atcaacagag cctgtatcaa ctcgttggag ccgttccccg cgatgatccc
    11341 atcagacctc cagccggcga agttcgcgag acattcgttt agttgggccg gaacgaagtc
    11401 gggatactgc gaccacttgc gcgccgcaaa ccggcgcaaa acttcctctt tgatatgagc
    11461 cggcgcatcc cagggattct cattttgatt aagcttcaac gaagcacgat gcgccggaag
    11521 cgtgtaggcc ttaaccgcgc gaacagaagg tttgattgaa tcgagcgctt ccttcataaa
    11581 ttttgtgctt tttgtgcctt ttcgtggcga tctttccgaa tcaacgcaga cctcgcatgt
    11641 cccgaaaggc cttcacactc agccagcacc gccacactct ccgccgctgc ggcaaatgcg
    11701 tctttcgaat accgcaacaa actcgttcgc ttcacgaaat cataaactcc gagcgccgag
    11761 gagaaccgcg ccgtccggct tgtcggcaac acatggtttg gaccagcgag ataatcacca
    11821 actgcctccg gcgtgtaact tccaaaaaat atcgcacctg catgtcgaat catggcgcca
    11881 atcgcttcat cgtcactcgt cacgatctca acgtgctcgg gcgcgagctc attcacgagc
    11941 gcacacgctt catccaacgt ctcaaccaaa acgatcgcgc cgtaatctct caacgacgcc
    12001 tgaacaatct cacgccgcgg cagcgattca gcctgacgcg ccacttcctc tgccacgtct
    12061 ctcgccagcg actcgctgtt cgtaatcaaa accgccgacg catcttcacc atgttccgcc
    12121 tgggccaaca agtccgccgc aatgaaatcc gcgcgcgccg tcttgtctgc aacaatcacg
    12181 acctcggtcg gtccggcaat cgcatcgatc cccactactc cgaacacgat cttcttcgcc
    12241 gccgccacgt acttgttgcc cggtccggtt atcttatcga cacgcggcac agtctgcgtg
    12301 ccaaaagcga gcgccgcaat cgcctgcgct ccaccaaccg cataaacctc gttaacgttc
    12361 aactcaacta acgccgcggc aacagcggaa ctctccgaca acgtgcgcgg cggagtcgta
    12421 accacgatgc gctcaacacc tgcgacctgc gccggcacaa cattcatcac caccgacgac
    12481 gggtacgcag ccgttcctcc cggcacatac aacccaactc gttcgagcgg cgtgatccgc
    12541 tggcccaaat gaaccccggc gcgcggattg atcgtccacg attcttcaac ctgccgctca
    12601 tgaaattctc tgacattcgc gatcgcctca cgcagcgcac gctgcacaca ttcatcgact
    12661 cccgccgcac accgccgcag ctcctcatca cttacacgta actgtcttag ctcaacctta
    12721 tcaaaccgcg ccgtgtaatc gatcagcgca tcatcaccac gcacacgcac atcatcaacg
    12781 atcgccccca caactttcat cagatccgca tcaaacgcca ccgagcgtgc cgccaggcgc
    12841 tcaaccagct ccatcttgtt aatcctgcaa atcatgcaat cctgtcttca actaactacg
    12901 tcctcaagca ccttaatcaa tcttccaacc tcatcactct tcaactggta actcgcgcga
    12961 ttcaccacca accttcccgt cgactccgca atctcttcaa ccactgtcaa cccgttctca
    13021 cgcaacgtca ccccagtctc aaccaaatca acgatcatgt ccgcgagtcc gagcgccggc
    13081 gcgagctcaa ccgatcccga taactgaata atctccaccg gtattccgcg tgccccaaaa
    13141 tgcgcgcgcg caatctgcgc atacttcgtc gccacacgaa tcatcccctg cacgttcgtc
    13201 atcgcttccg gatcagccgc agccacgacg atccgacaac gcgaaattcc gagatcgagc
    13261 ggcaaaagca gatccgcttc cgactcgagc aacacatcac gcccgacgat cccacagtcg
    13321 gcaatcccat gctcgacata aaccggcaca tccgcaggct tcacgaaaat aaaacgatac
    13381 gacagcgtct catccgtcag cgcgagacga cgcgagttca caccatcgtt caagcgaata
    13441 cccgcacgcg ccagcagcgc gagcgcgctc tcctgcattc gtcctttacc gattgcgatc
    13501 gttatcatct ttattgcgtc caagcacttc cgttagcgcg tcgagattga gcacgaaacc
    13561 tacagcaggc tcggcgcgtc cgaagctccc ggttagacca tcgtaacgac ctccacgccc
    13621 gacactcgct cccgcgccat gcacgaagat cttcaaactc aaaccggtgt agtagtcgag
    13681 actcgaaacg tcgccgagat cgatctcaaa tgagctctcg aaaccgagcg actcgatctc
    13741 ggtccagaga gcttcgaggg aatcgagggc ggcgagcgat cgcgcgtttg tgatcgcgtg
    13801 acgcgctgcc tgaataacgt tgcgtttacc ggttagttga gccggttgtg aaaaagcgcg
    13861 tgcttcggga ttgtcgtagt ttttgagaaa gcgctgtaac tcagccgttt cgcgtgtatc
    13921 gatcaaacgc cgcagttgtt cgcgcgcacg ctcttccaga tttaagtgcg ccgcgacgcc
    13981 gttgaagatc tcgacgttat tgatcgtgat gcagtaacgc gattgcagat tgagtcgcgc
    14041 caggatctcc gccgcgaggc gcagcacttc gagatctgcg ggcttgccgc cggtgccgat
    14101 cagctcacca ccgagctgcg tattctcgcg acgccattca gcgtgcgact gtgtttgctg
    14161 gcgaaagacc ggcgccgcat aacagaaacg cagcgggcgt ggtcgttcag aaagaagcgt
    14221 tgcggccatg cgtgcaaccg agctcgttac gtcaggtcgc agggcaagca gacgcccgtc
    14281 gttatcagtg aagcggaaac cgcgttgagc ctcacgctgg cccatgccct gttcgaagag
    14341 gtcataataa tcgaccgacg gcgtgatcac ttcctcgtaa ttccaggcct ggaagacaga
    14401 catcgcgaca tcttccaccg tgcggcgcag gcgcgcctcg gcgccgatgt aataacgcat
    14461 tccggctgga attctggaag tcataaaaaa caccaaatga aaaacccgct accggcttcg
    14521 tcgtcggtag cgggtttgga tatcgttttt gcgcttttta gcttgcttct gtcagcgccg
    14581 cgaacacgat cgccagcccg ccaccggtgt ggtggtgatg ccagttcgca tgatggccgc
    14641 gatcggtcgt aaacatgtct ttcggcttat acctgactcg cccgatactt gtcaaacatt
    14701 agacaggatt tacaagattt ctcaggatta acaagaaact tgttcatctt gaaaaatctt
    14761 gtaaatcctg tctaaaactc agccgcctgg aataaacggc cgcgattgca ctatgtagat
    14821 ctgacccttc atgtatgccc actcgatatc ctgatcgcgc gcgccaaaca cgcgcttgat
    14881 cgccgtcgcg gcccgcacca ggcgacgtat cacctcatcc gtcaacacta ctcgatcgcc
    14941 ttcgatcggc acttccttca caccgccgtt ctcatcaaac gtcaacaaac tatcttcagc
    15001 cgagcgcgtc aacacctgga tcgcattcgt gcgtggacga aagattatct gctccgcaat
    15061 gcgctgacct tcaaccacct tgatcccgag acctctcttc gcgctgatat agatcgcgcc
    15121 tttgttttca ttatcgaaag gatccgtgct gatcatcacg ccggagctct cggaattgat
    15181 tccctcctgt aacagcaccg ccatgaagat cttcgagtgg tccacgctcg cacgttcacg
    15241 cgcttcatac gcttcgaagt tccaaagtga cgcccagacc tttttgatcg catcaatcaa
    15301 cggctgttcg ccacgaacat taggcacagt cgtgtagaga ccggcgccac taaagttagg
    15361 caagtcttct gagtttgatg agctgcgcac aaacagtccc ttgtccggat attcacgcgc
    15421 aactttctgt aacaccattt ctttgagctt cggatcaaac tccgcgcttt cgatcttctg
    15481 ccgcagttgg accagttgtt cgcgccggta agcagggtcg tgtacaaact tctgatcgtt
    15541 cagtagtcca tagatcgtgt cgtcgaggtt gttgcgcttg atgaaatcgt cgtagtagta
    15601 aaaaggaatc gtgaaaccgc tcggcacgac aatgccgggc aaacgcgcgt tgagcacctc
    15661 gccgagattt gccgatttgc ctccgaaagc gagactcgat tttgaacgtt gctcagcgag
    15721 actcagcaga cgcgtttcat ccaggttgta gcgtggcttc atcacatcga gtcgttcgac
    15781 ctggcggcgt tgatactcgc gcaactggtt catgtcggca cgtctgatcg tgtagttctc
    15841 gcgcagcgtt tcgaacgaca cccaccagcc gtcatactgc ttcaacaact ctttcgcgtt
    15901 tttgatgtac gcgttgggaa tgttccaacc tttcgccagc aggttgatgt gcgaaagcgg
    15961 cgtcgatggc tgcgacgtga tgatgccggc gacgggcggg agttggaccg gtacttcgtc
    16021 gaggacgagg atctcgttga aaccgatctc aacgtggtcg tcgagcttcg gaatgatgtg
    16081 aatgcggccg aggcccttcg ccatgttaag cgcctgatac gcctgctcct ttgcgatgtc
    16141 gctttgcaac acgcgctgca taccggcgag cgagcgcgtc gcttcctctt gacgcagcga
    16201 gtttggtttg aacgcaaccg gggcaaagaa tgtcttgttg attacgtcgt aggcgagctg
    16261 gatctgatcg gcaggaatca aatcgccttc ccagaactca aagacccaac gcttgattgg
    16321 agtttgataa gcaagcgtac caagaatgaa gcgtcgattc ggtttgatgt agttgtttac
    16381 gaagaattct ctgccgcgct cgagggagag ataagtgccg ttgacgaagt ctttgtggaa
    16441 actgtagcgc tttttgttga cgtaatagat cttgttgccg tctttgcgat cgatcacgat
    16501 catgacatgc ggcaaagcgt agggcgtgtt tgcgtcgtaa gtgacggaga tcttgtcgaa
    16561 gtcagcttga gatctgatcg cgggcaaaga cttctgcgcc aaaacagcag ttttagccac
    16621 aaaaagcaca aaaagcacaa agaagacttt tttacttgtc tgagtcattt tatgtgcctt
    16681 ttgtctcttt tgtgcctttt gtgatctctt ctgccgactt cacgagcaat tctatcagac
    16741 cgtcgatgtg atgtttctct gttagaaagc tgttgacgtt cacacgcatc gcgcgtcgac
    16801 cgtgcagcac cgtggtggtc agccaggctt caccactgcg ctcgattatc tgctgcaggc
    16861 gttgttgtag acgatcggtt tccgctccat tcaatcgaaa acaacacacc gcagtctcga
    16921 cttcgccgac gcgtgtgaag ttttcgagct catcaatctt ctgcgcgaga tacctggtca
    16981 tatcgatctg atgttcgaca gtcctggcgt agccttcgcg gcccatgaac ttgagcgcca
    17041 tccagagttt caaagaattg aaacgacgcg tacccatctg cccgtaacgg aaaaagtcaa
    17101 actcgacgtc ggtgccgccg cgatcttcgc tcagatactc aggcgtcata tcgaacgcat
    17161 cgcgcagcac gcgcccgcca tcgcgcacca gtgtcgcgcc acacgagaac ggcacaaaca
    17221 tccacttgtg cggatcgaac gtgatcgagt ctgcgaggtt gataccccgc agcctgtctt
    17281 tgtgttttgg tgagaaagcg agtggtccac cataagcggc gtcgacgtga taccagcaac
    17341 ggttcgcgcg cgcgatctca gccagctctt cgagtggatc gatcacaccg gttgaggttg
    17401 tcccggcaac gccgacaatg cagcaaggta ttaatcccgc ctcacgatct ttcgcaactg
    17461 tttcgcgcag tgcatccgga attatgtgaa agcgatcgtc ggtcggaatc ttgcgcacgc
    17521 cttcgcgccc gagaccgaga atgtctgcgc tcttgtcgat cgagtagtga cactgttctg
    17581 aggcgtagat cacgagatcg cccggcgccg tgcgtacacc ccgatcctta atactcgccg
    17641 cgacactatc tcgcgcacac ttcagcgcga tcagattcgc ctccgaaccg ccgctggcca
    17701 gtgtgccgaa actctgtgga ccatagtcaa gcaggtcgca cagccagcgc atcacgcgcg
    17761 cttcgatcat cgcggaggtt ggaccattcc gccacgcgcc tgcattttga ttgagcatcg
    17821 aagagagagc atcagcccaa acgccgatcg gcaacggtgt gggattgaac tggccgaagt
    17881 atcgcgggct cgggacgccc atcgcattcg gcgctatctc gtttttgaac cgggccagga
    17941 tctcttcgag ctcgattccc ttctcgggca gcggctcgtc gaagagtttc tcgagctcgg
    18001 cgggcgtgga ctccggcgtc acccggcgcg tcgccagtcc ggccacatga tcggtaataa
    18061 tctgcgcgac cgcagggcct aagaaacgat gttgctcaac aaaatcctca atcatgattt
    18121 atgcttctcg atatgaacac gttcgagcaa tctttgttat cgaacatgga aagcttcgac
    18181 ctcgatctgt ctccggaaac ggtaacacag ctcggggaat actacagttt actcacacgc
    18241 tggaacgatc ggctacacct cgtcgcgccc tgttcggccg aggagtttgc ggtcaggcac
    18301 gtgctcgaat ctttgcttct tttgcgattt ttacctgcga acgctcgtgt ggcagacatc
    18361 ggctcgggtg gaggcttgcc gatcgttccc tgcctgatcg cacgaccgaa tttggaagct
    18421 acgttgatcg aatcttctca gaagaaggtc gtcttccttc gcgaggcttc aaatcgcctc
    18481 ggcattcgta cgagcatcat cgcgcgcccc ttcgaagacg ttcccgctcc gcgcgtttcc
    18541 ttcgtcacct gtcgcgcgtt ggatcagttc atggggaagc tgccggcgct gatcaactgg
    18601 gcgccacgag gcagtacgtt gctgttgttt ggtggtgaaa cgttgggtga gcaattgagg
    18661 cgtgcaagcg tgaacttcga acagctcctc atcccgcagt ctgagaaacg ctacctcttt
    18721 ttagccacgg attaaggcgg attgcataca cggatcgatc cgtgaatgaa atccgtgtaa
    18781 tccgtggcta agatggttct tccttaacga cgtcggttgg ttttttgtct tcgcggaggc
    18841 cctggaatga ggggtgacgg atgacgccct cgtcggtcca ctcggtgaac gcaacctcac
    18901 cgacgagctt cggctcggtc cagaccgcgt ggcgcaaacc aggatctttg gggatttcca
    18961 ggaaggggcg tttgtctacg gcgatgcggt cgagtttttt cttcaactca acccgcattg
    19021 tgtttgtgta accggtgccg gcgcgaccgg cgtaaatcag tttgcccttc tcgtagaaac
    19081 ccagaattaa agagctgaag gggattcctt tgtcagataa ggtatagccg gcaatcacga
    19141 actcctgccg cttgatacac ttgatcttca tccagttgcg actgcgcgtc gactcataag
    19201 ccgagtcagc cagcttcgag acgatccctt cgagcccaag cttgcaggct tccttgaaaa
    19261 actgcgcgcc gttaccttcg atgtgatcgc tgtagcgaag tgttccagtg tcgcccaacg
    19321 gctccagcaa ctccgccaac aaacgcttcc gctcgcgcag gggtgtcttc gtgatattga
    19381 acccttcgat atagatcaag tcgaacacgt tatagacaaa ccctgccgcg gctcccttat
    19441 tgatcgactg ctgcaaccgc tggaaactcg cacgacctga cggatccagc gccacgatct
    19501 ctccatcgag tatcgcgctc ttcaacggca acgctttgac ggccttcccg acactcgcaa
    19561 acttcaaggt ccagtctttt cgattgcgag tccagaaacg tacctggcgc ccttcgatat
    19621 gacagagcaa ccggtaacca tcgaacttca actcgtgaaa ccactcttca ccgctgggag
    19681 gtttagcggt gagtgtggcg agttggggcg gcacaaactc gggcatcgac gtcttgcgcg
    19741 cgcccggcag attcttgatc tgtttcgaat tcatgggagt ttcaatagcg gcatcgagat
    19801 tgcaaactca gcagattgac gtgctgagtt gtaccgaaag aagtcaaccc gagatgtatg
    19861 ctacacgcat gatgactgaa atttatcatc tggtccaacc acgcggacag cacactctgt
    19921 gtgggctaag gataagtcgt atcacgtcag aacgaaagac caacacgttg catctcgtga
    19981 atgatctgac gccaaacctg acgatttgca aacactgcga gaggatacag ggccaggatt
    20041 caaacggaaa ttaaaagccg cggaggcacg tggatgaacg ctgatctgcg tttatccacg
    20101 tgaatccgcg gcccagtttt cttcgtaact aatttacttc ttcgccgtcc gagccagcca
    20161 cgacgtcgcc gtcgctcgct gcagcaggca ccacgatcgg ctcgcgtgca ggcatcacga
    20221 tgtcgtgacc acccacgata tccggaaagt cttccagatc ccgctcacgc gcctgatcga
    20281 tcgtttcatc acgctcgata tcaacgttgc gatagtattc catgccggtt ccggccggaa
    20341 tcaaccgccc catgatcacg ttctccttca gcccgcgcag gtaatcaacg cggcccgaaa
    20401 tcgcagcctc ggtgagaaca cgcgtggtct cctggaacga cgccgccgag atgaatgaat
    20461 cggtcgagag cgaagccttc gtgataccca acaggagcgg ctcggcctgc gccacttcgc
    20521 catcttcctg tcgcacgcgc tcgttctcgt cctggaagcg gaaacgatcg acctgctctt
    20581 cgagcagaaa ctcggtgtcg ccgacttcct tgatcttcac ccagcgcaac atctggcgaa
    20641 caatcacttc gatgtgcttg tcgttgatgt t
//
LOCUS       DASSFQ010000225         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_546476, whole genome shotgun sequence.
ACCESSION   DASSFQ010000225 DASSFQ010000000
VERSION     DASSFQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546476"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFH96_08210"
     CDS             <1..881
                     /locus_tag="VFH96_08210"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HET6973993.1"
                     /translation="KFCSFCIVPYSRGRERSRAASEIIAEVKNLQTLGYKEIQLIGQN
                     VNSYRPRVEDGLEGFKGATPFCRLLRAVAATGMPRIKFTTSFPRDFHRDIVTAIEENP
                     NLCDWIHLPVQSGSDRMLKMMRRGHTRHDYWQRIERIKSSSRRLSLTSDIIVGFPGET
                     DEDFRQTLELVDQCEYDSIYIFKYSKRAGTPAADFTDDVPETEKKARFLALEEVQRRN
                     QERIYRSYVGRTVQVLVEKQSTRSESDLSGHSTCNKVVNFAGDRSLEGELVEITVTAA
                     KTNSLYGTLANRSAVE"
     gene            896..1387
                     /locus_tag="VFH96_08215"
     CDS             896..1387
                     /locus_tag="VFH96_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET6973994.1"
                     /translation="MEIEVKIRALMMDPNSGTPIIILKDVQSDTMLPIWVGSYEANAI
                     ALEIEKIAPPRPMTHDLLRNLIVELGVRVERVVVTSLRDNTFFAVIEMRNSEGDRMTL
                     DSRPSDAIALALRADCPIYVDMEVIKASQHTLPAEESEEGELSTSEEEWPDVIGEAGD
                     LPM"
     gene            1446..2201
                     /locus_tag="VFH96_08220"
     CDS             1446..2201
                     /locus_tag="VFH96_08220"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6973995.1"
                     /translation="MVIAIANQKGGVGKTTTAINLAAAFARKGKRVLLLDLDPQANSS
                     ISFLDPLTVERSVYELMMDGEAPIEQTVYGTPVENLSIVPARINLAKLESKLVGDFDA
                     PFRLKDRLEPFKEHYEVIVIDTPPTLGLITVNALVAASHVLIPIQSSYFALEGTDDLL
                     ETIEKVKARPNPNLELLGVLVTLHDKRTTLAREVHEQIRNVFGPKLFDTVITKSVRLE
                     ESPAYKESIFSFAPNSSGAIEYGKLCEEVISRV"
     gene            2194..>2719
                     /locus_tag="VFH96_08225"
     CDS             2194..>2719
                     /locus_tag="VFH96_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET6973996.1"
                     /translation="MSKRGLPTGLQMRHDSHYVEELSQHRPQQVGRMIPVDKLDPNPE
                     QPRTEFGDLTELTASIAEKGVLEPLLVKPNRLTGRWMIIAGERRWRSAQRAGLKEVPC
                     VEMEVDDGTVAEIALIENMQRKDLTVWEEADGLLALCDRFGYTHDDVARKVGKSRTTV
                     TEALAIAKIPEDVRE"
BASE COUNT          645 a          714 c          820 g          540 t
ORIGIN      
        1 acaagttctg ctcgttttgc attgttcctt attcgcgcgg ccgcgagcgc agccgtgcgg
       61 caagcgagat catcgctgaa gtaaaaaacc tgcaaacgct cggttacaaa gagattcaac
      121 taatcggtca gaacgtcaac agctaccggc cgcgtgtcga agacggtctc gaaggcttta
      181 aaggcgccac gccgttctgc cgtttgcttc gtgcggtagc cgcgaccggg atgccgcgta
      241 ttaagttcac gacgtctttc ccgcgcgact ttcacagaga catcgtcacg gctatcgaag
      301 agaatccgaa tctttgcgac tggattcatc tgcccgtgca atccggcagc gaccgcatgt
      361 tgaaaatgat gcgccgcggt cacacacgtc acgactactg gcagcgtatc gagcgcatca
      421 agagctcgag ccgccgtctg tcgctaacca gcgacatcat cgtcggcttt cccggcgaaa
      481 cggacgaaga ctttcgtcag acactcgagc tcgtcgatca gtgtgagtac gacagcattt
      541 atatcttcaa gtactcaaag cgcgccggaa cccctgcggc agacttcact gacgacgttc
      601 ccgagacaga gaagaaagcg cgctttctcg cactcgaaga agtgcagcgc aggaaccagg
      661 agcggatcta tcgctcgtac gtcggccgca ctgtgcaggt gctggtcgag aaacagagca
      721 cccgatccga aagcgatctc tcggggcact caacgtgcaa caaggtcgtt aattttgccg
      781 gcgatcgcag tctcgaaggc gagttagtcg agatcacggt gactgccgct aagacaaaca
      841 gtttatatgg aacgttggca aatcgttcag cagtagaata aggggtactg ctcttatgga
      901 aatagaggta aagatccgag cactgatgat ggatccgaat agcggaactc cgatcatcat
      961 tctgaaagac gttcagagtg atacgatgct cccgatttgg gtcggctcct acgaggcaaa
     1021 cgccatcgct ttggagattg agaagatcgc gccgccgcgg ccgatgacgc acgacctgct
     1081 acgcaacctg atagttgaac tgggagtgcg cgtcgaaagg gtcgtagtga cgagcctgcg
     1141 cgataacacg tttttcgccg tgatcgaaat gcgcaacagc gaaggcgatc gcatgacgct
     1201 cgactcgcgt ccgtccgatg ccatcgcgct ggcgttgcgc gcggattgtc ccatctacgt
     1261 cgacatggaa gtgatcaagg cttcgcaaca cactctgccg gcagaggaaa gcgaagaggg
     1321 cgagttgtcc accagtgaag aggaatggcc ggacgtcatc ggcgaagccg gcgacctgcc
     1381 gatgtgacga agtatgaacc caaagtcatg aaaacttgga gcgctagttt gccactgtgc
     1441 agcgaatggt aatcgccatc gccaatcaaa aaggtggggt gggaaagacc actaccgcta
     1501 ttaatctcgc cgccgctttt gcccgcaagg gaaaacgtgt ccttcttctc gatctcgatc
     1561 cacaggcgaa tagttctatc tcgtttctcg acccgctgac ggtcgagcga tccgtctacg
     1621 agctgatgat ggatggcgag gcgccgatcg aacagacggt ttacggaacg ccggtcgaga
     1681 acctgtccat cgtgcccgca cgaatcaacc tggcgaaatt agagtcgaaa ctggtgggcg
     1741 acttcgatgc gccgtttcgg ttgaaggatc ggctcgagcc cttcaaggaa cattacgaag
     1801 tgatcgttat cgacacgccg ccgacgttgg gcttgatcac cgtcaacgcg ctggtggccg
     1861 cgtcgcacgt gctgattccg attcagtcgt cgtacttcgc gctggagggc actgacgatc
     1921 tgctggaaac gatcgagaaa gtcaaagcgc gtccgaatcc gaatctcgag ctgctcggcg
     1981 tgctggtcac tttgcacgac aagcgcacga cgctcgcgcg cgaagttcac gaacagatcc
     2041 gcaacgtgtt tggtccgaag ttgtttgaca cagtgatcac gaagagcgtc aggctcgagg
     2101 aatcacccgc gtacaaggaa tcgatcttca gtttcgcacc gaattcatcc ggagccattg
     2161 aatacggaaa actttgtgaa gaggtaatca gccgtgtcta aaagaggact gccgacggga
     2221 ctgcaaatgc gacacgactc gcactacgta gaagagttgt cgcaacatcg gccgcagcaa
     2281 gtgggtcgca tgatccctgt ggacaaactc gatccgaatc cggagcagcc gcgcacggag
     2341 tttggcgacc tgacggagtt gacggcctcg attgcggaaa agggcgtgtt ggaaccgttg
     2401 ctggtgaaac cgaatcggct caccgggcgg tggatgatca tcgcgggtga gcggcgttgg
     2461 cgttcggcgc agcgtgcggg gttgaaggaa gtgccgtgcg tggagatgga agtcgacgat
     2521 ggcacggtgg cggagatcgc gctgatcgaa aacatgcagc gcaaggatct gacggtttgg
     2581 gaagaagcgg atggattgct ggcgttgtgc gataggtttg ggtacacgca tgatgatgtg
     2641 gcgcggaagg ttgggaagag ccggacgaca gtgactgagg cgctcgcgat cgcgaagatt
     2701 cccgaagatg tacgcgaag
//
LOCUS       DASSFQ010000226         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2050125, whole genome shotgun sequence.
ACCESSION   DASSFQ010000226 DASSFQ010000000
VERSION     DASSFQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050125"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1602
                     /locus_tag="VFH96_08230"
     CDS             94..1602
                     /locus_tag="VFH96_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6973997.1"
                     /translation="MLKTVWRYGLAVATVAVALFITKSLEQYTDITPLFYAAIVVSAW
                     FGGMGPGLLAVALSTVAIDYNFIEPLYTLNLGTKRITFLIVFGLLAVVTSWMSSRRRQ
                     SEEALRQARDELDARVQERTKELRQANEILRERADLLDLTHDTVFVRDMNDVITFWNR
                     GAEERYGWTREEAIGQVSHDIVKTAFPAPLSEINAELTRTGRWEGELLHTRRDGAVVT
                     VASRWALQRDDEGNPVAVLETNNDVTERKRAEEALQRVQAELTHATRVMTLGELTASI
                     AHEVNQPLAAIVTNGNACLRWLGGVQPNFLEARQAVERIIKDSYRASEVIARVRALVK
                     KSPPRNDLVDLNDVMVEVLALAQSQARRSHIFVKRQLANDLPPVLGDRVQLQQVVLNL
                     VINGLEAIAKSREGERELTVSSGKDEANNVIVAVRDTGTGIDPANLDRLFDAFFTTKP
                     DGMGMGLAISRTIIEAHGGRLAATPNSPKGSVFQFTLPQVSEKESSERESHD"
     gene            1595..2215
                     /locus_tag="VFH96_08235"
     CDS             1595..2215
                     /locus_tag="VFH96_08235"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6973998.1"
                     /translation="MTNAEAIVFVIDDDESIREALKSLIRSVGLSVETFGSAQDFLQR
                     QRPDIPACLILDVRMPGLSGLDLQRDLTEANIHLPIIFITGHGDIPMSVRAMKAGAVE
                     FLTKPFRDQDLLDAIQQALDRDRLARSQRADSAELLNRYHSLTPRETEVFGLVVKGML
                     NKQIALQLGTSEITVKLHRRQVMEKMHADSLADLVRMAEKLNTFGV"
     gene            2296..2715
                     /locus_tag="VFH96_08240"
     CDS             2296..2715
                     /locus_tag="VFH96_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6973999.1"
                     /translation="MNNAPTPNESKQARMPTPKVPLIAIVDDDDSVRDALKSLIDSIG
                     YRAEVFASGEAFLNSPYVSQTDCLIADVRMPGMSGLELQERLNASSTAVPIVFISAHD
                     DGEARAQGLRAGAVDFLKKPFSEDSLLGAIGACLEKS"
     gene            2875..>3130
                     /locus_tag="VFH96_08245"
     CDS             2875..>3130
                     /locus_tag="VFH96_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974000.1"
                     /translation="MFPASDDHCLTLNLGAPTFVSVLGRKGSVDGRIGSGEVAIIPPG
                     SSWTSRAEGADLPAMLLLYLRPLFVRSVVSEVDFAHNEIGL"
BASE COUNT          680 a          871 c          927 g          652 t
ORIGIN      
        1 cggtaacccc gcaggtccgt gtttccccaa aatccgtgtt acctttgggc gtggaagtac
       61 taaactgcca cgctcaaagg acacggattt aagatgttga agactgtgtg gcgatatggc
      121 ctggctgtgg cgacagtcgc agtggccctc ttcatcacaa aatcgctcga acaatacaca
      181 gacattacgc ctctttttta cgccgcgatc gtcgtcagcg cgtggttcgg cggcatggga
      241 ccaggtctgt tggcggtggc gctctctaca gtcgccatcg actacaactt tattgagccg
      301 ctctacacgt taaatctcgg aacaaagcgg atcaccttcc tgatcgtgtt tggattactg
      361 gctgtcgtaa cgagctggat gagcagcagg cggcggcagt cggaggaagc actgcgacag
      421 gctcgcgacg agcttgacgc gcgcgttcag gagcgaacaa aggaactgag acaagccaac
      481 gagattctgc gcgaacgtgc cgatctgctg gacctgactc acgacactgt cttcgtgcgc
      541 gacatgaacg acgtgatcac gttttggaat cgcggcgcgg aagagcgata cggttggact
      601 cgtgaagagg ccatcggcca ggtttctcac gacattgtga aaacagcatt tcccgcgcct
      661 ctgtcggaga tcaatgcgga gctcacgcgc accggccgtt gggaaggaga gctgctacac
      721 acgcgtcgtg atggagccgt ggtcactgtg gcgagccgct gggcgcttca gcgcgacgac
      781 gaagggaacc cggttgccgt gctggaaacc aacaacgacg tcactgaacg caagcgtgct
      841 gaggaagcat tgcaacgagt gcaggccgag ctcacgcacg cgacgcgggt aatgacgttg
      901 ggagaactga ctgcttcaat cgctcacgaa gttaaccaac cactcgcggc gatcgtcacg
      961 aacggtaatg catgtctgcg ctggctcggc ggcgtgcagc caaacttcct tgaggcgcgc
     1021 caggccgtgg agcgtattat caaagacagt tatcgcgcga gtgaggtcat tgcgcgtgtg
     1081 cgcgcgctgg tgaaaaagtc gccgccgcga aatgatctcg tcgatttgaa tgacgttatg
     1141 gtggaagtgc tcgcgttggc ccaaagtcag gcgcgccgca gtcacatctt tgtaaaacgc
     1201 cagctcgcga acgacttgcc gccggtgctt ggggatcgag tgcagttgca gcaggtggtt
     1261 ttgaacctgg tcatcaacgg tctggaagcg atcgccaaga gtagggaagg cgagcgagag
     1321 ttgacggtca gctcgggcaa ggatgaagca aataacgtga tcgtggcggt gcgtgatacg
     1381 ggtacgggga tcgatccggc gaatctggat cgcctgtttg acgcgttctt tacgaccaag
     1441 ccggatggta tgggcatggg cctggcgatc agccgcacga tcatcgaggc gcatggcggg
     1501 cgtttagctg ctacgccaaa ttcgcccaaa gggtcggtgt ttcagtttac gttgccccag
     1561 gtttctgaga aagagtcttc cgagagagag tcgcatgact aatgccgagg ccatcgtatt
     1621 cgtcatcgac gacgacgaat cgatacgcga ggcgttgaag agtctgatca ggtcggttgg
     1681 gctgagcgtg gaaacgttcg ggtcggccca ggactttctt caacgtcagc gtccggacat
     1741 tcccgcgtgt ctcatccttg acgtgcgcat gcccgggttg agtggtctcg atctgcaacg
     1801 cgatctcacc gaggcaaaca ttcacctccc gatcatcttc atcaccggtc acggcgacat
     1861 tcccatgtcc gtgcgcgcga tgaaagcggg agccgtcgaa tttctgacca aacccttccg
     1921 ggatcaggat ctgctcgatg ctatccaaca ggccctcgac cgcgatcgtc tcgcacgctc
     1981 acagcgcgcg gacagcgcgg agctcctcaa tcgctatcac tcgcttacgc cgcgcgaaac
     2041 ggaagttttc ggcctcgtcg taaagggaat gctgaacaaa cagatcgccc tccaactcgg
     2101 cacgagcgag atcaccgtca aacttcaccg ccgccaggtc atggaaaaaa tgcacgccga
     2161 ctccctcgcc gacctcgtcc gcatggccga aaaactcaac actttcggcg tttaggggca
     2221 ccataccaaa gtatagggtg cggttacgga cgtataatag ttttcgcttt ccacaaatcg
     2281 taatctccta ccgtaatgaa caatgcacct acaccgaacg agtcgaagca agcgaggatg
     2341 cccactccca aggttccgct gatcgcaatt gttgatgacg acgattcggt acgagacgcc
     2401 ctcaagagtt tgatcgattc catcggatat cgcgctgagg tgtttgcttc gggcgaggct
     2461 ttcctgaatt cgccttacgt ttcccaaacg gactgtctga ttgccgacgt ccgcatgccg
     2521 ggaatgagcg ggcttgaact tcaggagcga ctgaacgcct ccagcaccgc cgttccgatt
     2581 gtattcattt cggcccacga cgatggcgaa gcgcgggcac aagggttgcg ggcaggagcc
     2641 gttgatttcc tgaagaagcc gtttagcgag gattctctgc tcggagcaat cggcgcctgt
     2701 cttgagaaga gttaacgaaa aaggggacgt gtgatgttac aaactgctgc tactgctaac
     2761 tatcaatccc tggctgcgag atccgtaaag ccccggtcgg tgctgaaatc gagcgacgcg
     2821 atcggctggc aaaacgtgcg tgccatacat tttcgccaca cctcacgaga ggcgatgttt
     2881 cccgcttctg acgaccattg cctgaccctg aacctgggcg cgccaacgtt tgtcagtgtg
     2941 ctcggccgca aaggaagtgt cgacggccgg atcggctcgg gcgaagtcgc aatcattccg
     3001 ccgggctcat cgtggacctc ccgcgccgag ggcgcagatt tgccggcaat gctgctgctc
     3061 tatctgcgcc ctttgtttgt gcgttcggtc gtgagcgaag tggacttcgc ccacaacgag
     3121 atcggtctga
//
LOCUS       DASSFQ010000227        11125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2052254, whole genome shotgun sequence.
ACCESSION   DASSFQ010000227 DASSFQ010000000
VERSION     DASSFQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052254"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFH96_08250"
     CDS             complement(<1..1011)
                     /locus_tag="VFH96_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HET6974001.1"
                     /translation="MSVLVANCPSCGAPIEFKSGQSIVVICQYCHSAVARTDRELKDL
                     GKVAELVETGSPLDVGLRGTWKDVPFELTGRAQLGHEMGGQWDEWYATFSNGWLGWLA
                     EAQGRFYITFQYPIPEGTYVPTFEQLQLGQEVYGLPWNTPLMVAETGRATALGAKGEI
                     PYLLTPGETYDYADLSGANGAFGTIDYNQTPPLIFLGQQVTLADIGITTTRAPEKEER
                     RVDAAQLGCPNCGGPLELRAPDKTERVTCPNCNSLLDVNQGKLQFLKALEKPWFQPVI
                     PLGTTGEVPEGKMTVIGAMARSVTIEGTQYFWSEYLLYQPQIGFRWLVDSDDHWNYVR
                     AVP"
     gene            complement(1008..1379)
                     /gene="speD"
                     /locus_tag="VFH96_08255"
     CDS             complement(1008..1379)
                     /gene="speD"
                     /locus_tag="VFH96_08255"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HET6974002.1"
                     /translation="MTLGTEWLIDASGCDPTALADLDRLREIFQRVIRDLGLNVIGEI
                     AWHQFDQPHGVSGLALLSESHLTCHTYPEFRAATFNLYCCRNRASWSWEATLKQMLGA
                     TEVNVRVFDRTIEDVAAGVAK"
     gene            complement(1437..4322)
                     /locus_tag="VFH96_08260"
     CDS             complement(1437..4322)
                     /locus_tag="VFH96_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6974003.1"
                     /translation="MKPLPVQTIAHYRIVEPIGAGGMGAVYKAYDNKLQRTVALKLLP
                     SEYVSQQDRRRRFFQEARAASALSHPHILTIYEVGEDNGTPYIAMEYIEGQTLRQKIK
                     KNGLQLKETLDIAIQIAAGLARAHESGIIHRDLKPENLMLSRDGYAKILDFGLAKLVA
                     ERERALVADSEQKTLVLGVQTESGTLLGTVNYMAPEQLLGQRVDRRCDVFSFGVVLCE
                     MLTGTAPFVHDNRIDTMHAILHRDPRFPSDGRPELPFDLQRVLTKALAKTPKDRYQTI
                     DELAEELKTLKRNLELGKTLPVTPRTRLVLKRTAAGARTVDYEKELNEAQFKAVTTTE
                     GPLLIVAGAGTGKTRTLVYRVARLVEIGAKPESILLLTFTRRAAASMLTRAAALADAR
                     CQRVSGGTFHSLGHSVLRKFADEAGVAKNFTVLDQSDTEDLIDLLRRQIRLTKAQHFP
                     RKRTIGAMFSMMVNKVLSLKQVLNQHYPQFVDERSNLETLFKTFEEFKRSRHMLTYDD
                     LLVRFREALENSATMREQISDQYRYIMVDEYQDTNKLQAQIVKLMTARHDNVAVVGDE
                     FQSIYSFRGASHRNMLEFPKLFPSSKIIKLEENFRSTQPILDVANAIISDVKESYKKR
                     LYSRIDGGQAPVVVSALDENEQSRFVSQRIADLREEGVPLSEIAVLFRSSSHSFDLEI
                     ELGRLGIPFRKFGGIRFAESAHIKDALSFLRVVVKPSDTLSWFRALKLIDHIGDATVY
                     QILEHLGVEQREFRSPRSKAGLFKKLQRFPARAAYQAQLQRVARVFVAMLESKSPNEQ
                     LSAVLRFYKPLLKNKYDDVQRRGRDLEHLQSIAKRYKTAAKLLDDIALDPTEVVQENV
                     PRRSSGFVTLSTVHSAKGLEWDNVFLIWMTDGWFPSSRFQDEFDDYEEERRLLYVATT
                     RAKKDLHFVYPAVDVYRGPETDGTPTLSRFLEPIPPSILPHASLAGE"
     gene            4514..5116
                     /locus_tag="VFH96_08265"
     CDS             4514..5116
                     /locus_tag="VFH96_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974004.1"
                     /translation="MICPRCASNQGDDIKFCTSCGANLQAVREAMQVADSSAKFDWGN
                     TWVAEMFMSGQAHELRKQEMERRLGITPQVKRYNEIKAGVIVSSVGIGVSIFLFFIMH
                     AIANQNPRDAELLSSIWLAGVIPFMVGLALIINGVVVSKKMVEVMDREQHKAKSLDEG
                     SAPSPRGLKSADTNEFIPANYSVTDSTTRHLESSERKASR"
     gene            complement(5174..6073)
                     /locus_tag="VFH96_08270"
     CDS             complement(5174..6073)
                     /locus_tag="VFH96_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET6974005.1"
                     /translation="MPELPDIVVYIEALEKRIQGSVLERVRIASPFLLRTAVPPLSSV
                     EGKKIVELRRLGKRICFGLEGDLWLVLHLMIAGRLHWYNERAKAAKGRSLAVFDFSGG
                     SLVLTEAGTQKRASLHVVQGEAGLDNLDPGGLDVFDATFDDFARAIRAKNHTLKRALT
                     DPRILSGIGNAYSDEILFEARLSPFTLTQKLSDKDVENLLESIKRTLTDWLERMRVES
                     RDRFPEKVTAFRPDMATHGKYGEPCPRCGNKIQRIRYGSNETNYCPTCQTGGKLLADR
                     ALSRLLKDDWPRTPEELEMMTKK"
     gene            complement(6089..6352)
                     /locus_tag="VFH96_08275"
     CDS             complement(6089..6352)
                     /locus_tag="VFH96_08275"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET6974006.1"
                     /translation="MMNFIWWLIIGLIAGALARLIMPGRDAMGIIATIILGIVGSLVG
                     GLVSMAIWGNNTAGFQPAGLLLSILGAIIVLWIYRMVKGRSTV"
     gene            complement(6460..7854)
                     /locus_tag="VFH96_08280"
     CDS             complement(6460..7854)
                     /locus_tag="VFH96_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6974007.1"
                     /translation="MKRARVICWLLVFCLGFLITQTIANPRAIKPAIDFTELDKLIPT
                     ELKEKNTPGTVITVISGDQIIYQKAFGVANVETNAEMQPEMLFRLGSTTKMFTAAALL
                     TLAENNKIKMNEPIGNRVKGLNARLGQVTPHHLLSNSAGIRDFAAPVISNDDPSLGNM
                     VRGWKEDVFFADQGEIYSYSSPGYWLSGFVVEELYGKPYADAMTELLFKPIGMERTTL
                     RPFMAITYPFATGHVVQEGKATIIRPMFNNVAMWPAGSIFSNARDLSRWVIALMNEGK
                     VESKQLLPASVINQMVGHHVAVPGESDSYYGYGLTVFKYKGLEFVGHGGFSRGYGSMI
                     QMVPSKKFAVIVLTNKSGETMRKSLNKAMELGLGLKDDEEPKPAPVAPPATSEMSEYA
                     GTYSHAPQTWEVSIKDGKLFMKFDEKDYAMTKSGERKFTFGAQNENEVIFVAGKDGKI
                     HFLFTELYGAKKIR"
     gene            8225..8797
                     /locus_tag="VFH96_08285"
     CDS             8225..8797
                     /locus_tag="VFH96_08285"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HET6974008.1"
                     /translation="MFLRKYATRLLATAVISILLAPTLFNLKSTATQTQGQSTCSDGW
                     YITGYYVPREDELPDTTEQINVERVGDLSFSQKFLNEIRTEGWGITRFGWALGYYSNG
                     WHRSDAGALDASGNPLIVGAIAIDRSVIPRGAHVQIATLPSPWASKTFRATDIGNGIV
                     GQHIDVFTGIGLAAEEETFRITSNNNRVCF"
     gene            8880..9896
                     /locus_tag="VFH96_08290"
     CDS             8880..9896
                     /locus_tag="VFH96_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974009.1"
                     /translation="MVVSASQIDPSLLPFLDANNAPEEAAALARLNAEEIEPTIRRGL
                     GYKLGFSRPELEELHSDIQLRLLKRLRALKQDPAGNQIVNLRGYVATVTRNTCDEYLR
                     QRYPLRRSLRDKVRRHLLSHAEFALWEDNDNWLTGLSDWHDHKLNEELSELQERLKTE
                     WQSVDVQRLNLQELLTTIFRVARGPINLDALTALIAELWGVEDRPAESLDAVTNATLD
                     EQSGVDINPATIIERRQSLQLLWREICELPRRQRVALLLNLRNPNGINVITLLPATGV
                     ATFEQIAQTLEIPANEFEQLWARLPLDDLFLADYLGGTRQQVINLRKTARERLLKRMN
                     GLKA"
     gene            9893..11089
                     /locus_tag="VFH96_08295"
     CDS             9893..11089
                     /locus_tag="VFH96_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974010.1"
                     /translation="MMEHPTKRELDEYRSRVLAPAAFLSIHRHVATCPGCAAQCPDDL
                     ARDLEVLHTALLQDETPYHLSPAEVAAYARGTANEIDLEIADSHLSDCAPCRNEVQRH
                     APAEVKMFKPQRLSFTNAWRIAAAVACAIIVIGLPIWLIRSKPAPHEGQVANPANTST
                     PAPEVQPSLEVVPEFALVLNDGNRKVTVDKQGTLAGLELLPSPVQQKVRAALQTGRLE
                     QSSALAQVKTERSTLLGMSNGGLPFPIIGPLGEVVRSERPTFRWHSSGGVQSYTVTVT
                     DADLNVIATSPPLKTTQWRISKSLKAGGIYAWQVTALKDDGTRITAPVLPAPQAKFKI
                     VDRSIAEMLQQAKSTYPDSHLTLAVLYAEAGLIDDAEQELRMLIRDNPQAGIAQKLLR
                     QVQAMR"
BASE COUNT         2358 a         3036 c         3179 g         2552 t
ORIGIN      
        1 cggaaccgca cgcacgtagt tccagtgatc gtcgctatcg accagccagc gaaatccgat
       61 ctgcggctgg taaagcaggt actcgctcca gaaatattgc gtgccttcga tcgtcacgct
      121 gcgcgccatc gcgccaatga cagtcatctt tccttccggc acttcgccgg tagtcccgag
      181 cggaatcacc ggctgaaacc acggcttctc gagtgctttc agaaactgca gcttgccctg
      241 gttgacatcc agaagcgagt tgcagtttgg acaagtgacg cgctcggtct tgtcaggcgc
      301 acgcagctcg agtggaccac cacagtttgg acaacccagt tgcgcagcgt cgacgcgtcg
      361 ctcttctttt tcaggagcac gcgtcgtggt gataccgata tccgcaagtg tgacttgctg
      421 gccgagaaag atgagtggcg gcgtttggtt gtaatcgatc gttccgaatg cgccgttcgc
      481 gccggagaga tctgcgtaat cgtaagtttc accgggcgtg agcaggtaag gaatctcgcc
      541 tttagcgccg agtgcggtgg cgcgtccggt ttccgcaacc atcagcggag tgttccatgg
      601 caagccgtac acttcctgtc cgagctgtag ctgctcgaat gttggtacgt acgtgccttc
      661 gggaatcgga tattgaaacg tgatgtagaa gcggccctgc gcttccgcga gccagccaag
      721 ccagccgttc gaaaacgtcg cgtaccactc gtcccactgg ccgcccatct cgtgtccgag
      781 ctgcgcgcgg ccggttaact caaacggaac gtctttccac gtgccgcgca gtccgacgtc
      841 cagtggcgat ccggtttcga caagctccgc gaccttgccg agatctttca gttcgcgatc
      901 ggtgcgcgcc accgcggagt ggcagtattg gcaaatgacg acgatggatt gcccgctctt
      961 gaattcaata ggcgcgccgc acgacgggca gttcgccacg agcacgctca tttagcgact
     1021 cctgcggcga cgtcttcgat cgttcgatcg aatacacgaa cgttcacttc cgtggcgccg
     1081 agcatttgtt tgagcgttgc ttcccaggac catgaggcgc gattgcgaca gcagtagaga
     1141 ttgaaggtcg cggcgcgaaa ctcgggatag gtgtggcagg tgagatgcga ctccgaaagc
     1201 agtgcgaggc cgctgactcc gtgtggttga tcgaattgat gccaggcgat ctcgccgatg
     1261 acgtttagac cgagatcgcg gatgacgcgc tggaaaattt cacgcaggcg atcgagatct
     1321 gcaagggccg tcgggtcgca gccgcttgcg tcgatgagcc attcggttcc tagagtcata
     1381 agccatccac agattgcaca gattatcata ttgtctgtgt aatctgtgga tggcttttat
     1441 tcacctgcca gcgatgcgtg tgggaggatt gagggcggta tcggttcgag gaagcgcgag
     1501 agtgttggtg tgccgtccgt ctccggtccg cggtagacgt cgaccgccgg atacacaaag
     1561 tgcaagtcct ttttcgcgcg cgtggtggcg acgtaaagca gccgtcgctc ctcctcgtaa
     1621 tcgtcaaact cgtcctgaaa cctgctcgac ggaaaccagc catcagtcat ccagatcagg
     1681 aaaacgttat cccattcgag tcctttcgct gaatgcacgg tcgagagcgt cacaaaaccg
     1741 ctgctacgtc gcggcacgtt ttcctgtacc acttcggtcg gatcgagcgc gatgtcgtcg
     1801 agcagtttcg cggccgtttt gtagcgcttc gcgatcgatt gcaggtgctc gagatcgcgg
     1861 ccacgtcgct gcacgtcgtc atatttattc ttcaacagcg gcttgtagaa gcgcagcact
     1921 gcagacaact gctcgtttgg cgatttgctt tcgagcatcg cgacgaacac ccgcgccact
     1981 cgctgcagtt gagcctggta agctgcgcga gcagggaagc gctgaagttt cttgaacagt
     2041 ccggccttgc tgcgcggcga tcgaaactct cgttgctcga cgccgagatg ttcgagtatc
     2101 tggtaaacgg tggcgtcgcc gatgtgatcg atcagcttga gcgcgcgaaa ccacgagagc
     2161 gtgtcagatg gcttaacgac cacacgcaga aacgagagcg catccttgat gtgcgccgat
     2221 tccgcaaacc ggataccgcc aaacttgcga aacggaatgc cgagcctgcc gagttcgatc
     2281 tccagatcga atgagtgcga gctggagcgg aacagcacgg cgatttcact cagcggcacg
     2341 ccctcttcgc gaagatcggc aatgcgctgt gagacaaacc gtgattgttc gttctcgtct
     2401 agcgcgctca ccaccaccgg cgcctgccca ccgtcgatac gcgagtaaag ccgtttcttg
     2461 tagctctcct tgacatcgga aatgatcgcg ttggcgacgt cgagaatcgg ttgcgtgctg
     2521 cggaagtttt cttcgagctt gatgatcttc gatgacggga agagcttcgg aaactcgagc
     2581 atgttgcgat gactcgcgcc gcggaacgag tagatcgact gaaactcatc accgacaacc
     2641 gcgacgttat cgtgtcgcgc ggtcatcagt ttcacgatct gcgcctggag tttgttcgtg
     2701 tcctgatact cgtcgaccat aatgtagcga tactggtccg agatctgttc gcgcatcgtc
     2761 gcgctgtttt cgagcgcctc gcggaatcgc acgagcagat cgtcgtacgt cagcatgtgc
     2821 cggctgcgtt tgaactcctc aaacgtcttg aacaacgttt cgaggttgct tcgctcgtcg
     2881 acgaattgcg ggtaatgctg gttcagtacc tgcttcagag acagaacttt gttgaccatc
     2941 atgctgaaca ttgcgccgat ggttcgcttg cgtgggaagt gttgcgcttt cgtgagccgg
     3001 atctggcgtc gtaggagatc gattagatct tcggtgtcgc tttgatcgag cacggtgaaa
     3061 tttttggcga caccggcttc gtctgcgaac tttctcaaga ccgaatggcc caaagagtga
     3121 aacgttccgc cggagacgcg ctggcaacgc gcatcggcga gagcagcggc gcgcgtcaac
     3181 atgctggcag cggcgcggcg cgtgaaagtc aataagagaa tcgactcggg tttcgctccg
     3241 atttcgacga gccgtgcgac ccgatagacg agcgtgcgcg tctttccagt gcctgcaccg
     3301 gcgactatca gcagcggacc ttcagtggtc gtgactgctt tgaactgcgc ttcgtttagt
     3361 tccttctcgt aatcgactgt gcgcgcgcct gcggcggtgc gtttcaacac gagtcgcgtt
     3421 cgtggtgtta caggcagtgt cttgccgagc tcgagattgc gtttgagtgt tttgagctct
     3481 tcggcgagct cgtcgatcgt ttgataacgg tctttcggtg tcttggcgag cgcctttgtg
     3541 agtactcgtt gcagatcgaa aggaagctcc ggacggccgt cgctcggaaa gcgtggatcg
     3601 cgatgcagga tcgcgtgcat cgtgtcgatg cggttgtcgt gtacgaacgg cgccgtgccc
     3661 gtcagcattt cgcagagcac cacgccaaac gagaatacgt cgcagcgacg atcgacgcgt
     3721 tggcccagca actgttcggg cgccatgtaa ttgacggtgc ccagcagtgt gcctgattca
     3781 gtctgaacgc cgagcacgag tgttttctgt tcgctgtcag cgacgagcgc gcgctctcgc
     3841 tcggcgacga gttttgcgag tccgaagtcg agaatcttag cgtagccgtc gcgactcagc
     3901 atcaggttct cgggtttcag atcgcggtga atgatgcctg actcgtgtgc gcgtgcgagt
     3961 ccggcagcga tctggatcgc gatgtcgaga gtttctttga gttgcaggcc gttctttttg
     4021 atcttctggc gcagcgtctg cccttcgatg tactccatcg cgatgtaagg cgtgccgttg
     4081 tcttcgccga cctcgtaaat cgtgagtatg tgtggatggc tcagcgccga ggccgcgcgt
     4141 gcttcctgaa agaaacggcg acggcggtct tgctgcgaga cgtactcaga tgggaggagt
     4201 ttgagcgcga cggtgcgctg gagcttgttg tcataagctt tgtagacggc gcccatgccg
     4261 cccgcgccga tcggctcgac gatccggtag tgcgcaatgg tttgaaccgg gagaggcttc
     4321 atgtcgtaat tttagccgca gatagacgca gatgatacag atatgttctg atctatgtga
     4381 atcaggccag agaaggacgt agtgggtcag tttgatgtcc gataagcttt agcttgtcgg
     4441 catgtcgcga caaactaaag tttatcggac acttgcagcg aaagtgaacc actacccaga
     4501 gaaggagtta cttatgatat gtccgcgttg tgcgtcaaat cagggtgacg acataaagtt
     4561 ttgcacgtcc tgcggcgcga acctgcaagc ggtgcgtgag gctatgcaag ttgccgactc
     4621 gagtgcgaag tttgactggg gcaacacgtg ggtggccgag atgttcatgt cgggccaggc
     4681 tcacgagctc cgcaaacagg aaatggagcg ccgtctcggg atcacgcccc aggttaagcg
     4741 ttacaacgaa ataaaggctg gtgtgatcgt cagcagtgtc ggcattggcg tgtcgatctt
     4801 tttgtttttc ataatgcatg cgattgcgaa tcaaaaccct cgcgacgctg aacttctcag
     4861 cagcatctgg ctggcaggag tcattccttt catggtgggg ctcgcgctca ttattaatgg
     4921 cgttgtagtg agcaagaaga tggtcgaggt gatggaccgc gagcagcaca aggcgaagag
     4981 tcttgacgag ggatcggctc cgtctccgcg tggtctgaaa tcggcggaca cgaacgagtt
     5041 tattcctgca aactatagcg tgacggactc caccacccgg catctcgaaa gttctgaacg
     5101 gaaagcgagt cgttagccac aaaaaggcac aaaaagaaag ttcttctttt gtgctttttg
     5161 tgccttttcg tggctacttc ttcgtcatca tttcgagctc ttccggggtg cggggccagt
     5221 cgtcctttag gagacgtgag agggcgcggt cggcgaggag tttgccgccg gtctgacaag
     5281 tcgggcagta atttgtttcg tttgagccgt aacggatgcg ctggatcttg ttgccgcagc
     5341 gtgggcatgg ctcgccgtat tttccgtggg tggccatgtc gggacggaag gcggtcactt
     5401 tctcaggaaa gcgatcgcgt gattcgacac gcattcgttc gagccagtca gtcagcgtgc
     5461 gcttgatcga ctctaagagg ttctcgacgt ctttgtcgga aagtttctga gttaacgtga
     5521 acggagagag ccgcgcttcg aagaggatct catcggagta agcgttgccg atgccgctga
     5581 gtatgcgcgg atcggtgagt gcgcgcttca gtgtgtgatt cttcgcgcgg atcgctcgcg
     5641 caaagtcgtc gaatgtggcg tcgaaaacat cgagaccgcc gggatcgaga ttgtccaatc
     5701 cggcctcacc ctggaccaca tgaagcgaag cacgcttctg tgtcccggct tcagtcagga
     5761 ccagactgcc gccgctgaaa tcaaatacgg ccagacttcg acccttagca gcctttgcgc
     5821 gctcgttgta ccagtgcagc cggcctgcaa tcatcaggtg caacacgagc cacagatctc
     5881 cttcgaggcc gaagcagatc cgcttgccaa gtctgcgcag ctcgacgatc tttttgcctt
     5941 cgacgctgga cagcgggggc acggccgttc gcagtagaaa aggactggcg atccggacgc
     6001 gttcgagaac actgccttgt attcgtttct cgagagcctc gatataaacg acgatgtccg
     6061 gcagttcagg cactaattgt aactctgctt aaacggtact gcggccttta accatccgat
     6121 agatccaaag cacgatgatg gcgccgagta tcgaaagcag cagaccggcg ggttggaatc
     6181 cggcggtgtt gtttccccag atggccatgc ttacgagacc accaaccagg gagccgacga
     6241 tacccaatat gatggtggca atgatgccca tcgcatctct gcccggcatg atcagacgcg
     6301 ccagtgcacc tgcgatcaat ccaatgatta accaccaaat gaaattcatc atctgttttg
     6361 tcctccgtgg tgcgctttat atcactcctt tgactcaata agaagtcgca agaaacacac
     6421 acaaggaatt agtggaataa atccccgttt taatccgcgt taccggattt tcttcgcccc
     6481 gtaaagctcc gtgaataaaa agtggatctt cccatccttc ccggccacaa agataacctc
     6541 gttctcattc tgcgcgccga acgtaaactt ccgttcgcca ctcttcgtca ttgcataatc
     6601 cttctcatca aacttcataa agagctttcc atctttgatc gagacctccc acgtctgcgg
     6661 tgcgtgcgag taagtgccgg catattcact catctctgac gttgcgggcg gcgccaccgg
     6721 cgccggcttc ggttcctcat cgtcctttaa tcccagcccc agctccatcg ctttattgag
     6781 actcttccgc atcgtctcac cactcttgtt cgtcaacact atcaccgcaa acttcttcga
     6841 cggcaccatc tggatcatcg atccgtaacc gcgactgaat ccgccgtggc ccacaaactc
     6901 cagcccctta tacttgaaca ccgtcaaacc gtagccgtaa tacgaatcgc tttcaccggg
     6961 cacggccacg tgatgcccga ccatctgatt gatcactgac gccggcagca gttgcttgct
     7021 ttcgactttg ccttcgttca tcagcgcgat gacccagcgc gacagatccc tggcgtttga
     7081 gaagatcgaa ccggcgggcc acatcgctac gttattgaac atcggccgga tgatcgtggc
     7141 cttgccttcc tggaccacgt ggcccgttgc gaatggataa gtgatcgcca tgaacggccg
     7201 cagtgtggtt cgttccattc cgatcggttt gaacagtagc tcggtcatcg catcggcgta
     7261 aggcttaccg tacaactctt cgaccacgaa gcctgacagc cagtagccgg ggctcgagta
     7321 ggaatagatc tcaccttgat cggcgaagaa gacgtcttct ttccagccgc gcaccatgtt
     7381 gccgagtgac gggtcgtcat tcgagattac aggcgcagcg aaatctcgaa taccggcgga
     7441 gttgctcaag agatgatgcg gtgtgacctg gcccaacctc gcgttcagac ctttcacgcg
     7501 attgccgatc ggctcgttca tcttgatctt gttgttttca gcgagcgtga gcagcgcagc
     7561 ggccgtgaac attttggtag ttgaaccgag acggaagagc atctccggtt gcatctctgc
     7621 attggtctcg acgttcgcga cgccaaacgc tttttgataa atgatctgat cgccactgat
     7681 gacggtgatc accgtgccgg gtgtgttctt ttctttgagc tcggtgggga tcagcttgtc
     7741 gagttcggtg aagtcaatcg ccggcttgat ggctctgggg tttgcgatcg tctgagtgat
     7801 gagaaagccg agacaaaaca ccagcaacca gcagattacg cgagctcgtt tcattattat
     7861 tcgtctcctt gaggattgcg gctgagacac acgaccgcat gcaagcgctc cttatacgca
     7921 cgcacccgga tcagggttct tggatagcgg tctaaataaa tggagaagga ctgttcagtt
     7981 gatcctcagc cgcggataga cgcagatgaa cgctgatctg cgtttatccg cgtgaatctg
     8041 cggtcgacaa atgcctcgac ttcggctatg cgatcgtgag gaagtctctt tatcttttcg
     8101 attaattcca tgggcgaagt ataacattgt tacccaagtc cgaggcatcc ttgccacaaa
     8161 aaataaaaac taaaacaagg tgtcttcaat cgaaatctca ggttggaatg agtatcttgc
     8221 aggtatgttt ttacggaagt acgcaacacg actgctggcg accgcagtca tctctatatt
     8281 attggcgcca actctattca acttgaagtc aacggcgaca caaacacaag gacaatcaac
     8341 ttgctcagac ggctggtata tcaccggcta ttacgtcccg cgtgaggacg agctgcctga
     8401 tactaccgaa cagattaacg tcgagcgtgt tggcgatctc agtttcagtc aaaaattcct
     8461 aaacgagatt cgcaccgagg gttggggcat cacgcgcttc ggatgggcgc tcggctatta
     8521 ctctaacggc tggcatcgct ctgacgccgg cgcgcttgat gcttccggaa acccactgat
     8581 tgtcggcgcg atcgcaatcg atcgctccgt catcccacga ggcgcccacg tgcaaatcgc
     8641 gaccttgccg agtccgtggg cgtcgaagac tttccgcgct acggacatcg gtaacggcat
     8701 cgtcgggcag cacatcgacg tgtttacagg catcggtctc gcggcggaag aggaaacgtt
     8761 tcgcatcacg tcaaacaaca accgggtctg cttttgattt taagacgtta ttagcaagtg
     8821 gtagactgcc acgagtttcc tgttcccgga agtctctccc cctgcgacag tttaatcgtg
     8881 tggttgtaag tgcctcacaa atcgatccct cgctgctgcc ttttctcgat gcgaataacg
     8941 cgcccgagga agctgcagcc ctcgcacgcc ttaacgccga agagatcgag ccgaccattc
     9001 gccgcggttt aggatacaaa ctcggattct ctcgccccga gctcgaagaa cttcatagcg
     9061 atattcaact gcgcttgctc aaacgcctgc gcgcgttgaa acaggacccg gccggaaatc
     9121 agatcgtgaa cctgcgcggc tatgtcgcga ctgtcacgcg caacacgtgt gacgaatacc
     9181 tgcgacaacg ttacccgctg cgacggagct tgagagataa agttcgtcgc catttgctca
     9241 gtcacgctga gtttgccttg tgggaagata acgacaactg gctcaccggg ctttccgatt
     9301 ggcacgacca taagctgaat gaagaactga gcgagttgca ggagcgtttg aaaaccgaat
     9361 ggcagtcggt agatgttcag cgtctcaatc ttcaagagct gctgacaacg atcttccggg
     9421 tcgcgcgtgg tccaatcaat ctcgatgcgc tcacagcgct catcgccgag ctctggggcg
     9481 ttgaagatcg accagccgaa tcgctcgacg ccgtcacgaa tgccacgctc gacgagcaat
     9541 ctggtgtgga catcaatccc gcgacaatca tcgaacggcg gcagtcgttg caactgcttt
     9601 ggcgtgaaat ctgcgagttg ccgcgccggc agcgcgtcgc gctactgctt aatttgcgga
     9661 acccgaacgg cattaacgtt atcacgctat tgccggcaac gggtgtcgcg acgttcgaac
     9721 agatcgcgca aacattggaa atacctgcga acgagtttga gcaactctgg gccaggttgc
     9781 cgcttgacga tctgtttctt gcggactatc tcggcggcac gcggcaacag gtgatcaact
     9841 tgcgtaagac agcgcgtgaa cgcttactca agcgcatgaa tggactgaag gcatgatgga
     9901 gcatccgaca aaacgggaac tggatgaata tcgctcgcgc gtgctggcgc cggcggcctt
     9961 cctctctatt caccggcatg ttgcgacttg tccgggttgc gccgcgcagt gtcctgatga
    10021 tctcgcgcgt gacctcgagg ttctgcacac agcattgctt caggacgaaa cgccatatca
    10081 cctctctccg gccgaagtgg cggcttacgc gcgtggcact gcaaacgaga tcgatttgga
    10141 gatcgctgat agtcatttga gtgattgcgc gccgtgtcgt aatgaggtgc aacgacatgc
    10201 gccggctgaa gtgaagatgt tcaaaccaca acgcttgtcg ttcacgaacg cgtggcgtat
    10261 cgcggcagcg gtggcttgcg cgattatcgt gatcgggttg ccgatctggt taatccgaag
    10321 caagcccgcg ccacatgaag ggcaagtggc gaatccggct aacacgagca ctccggcgcc
    10381 tgaggtccaa ccgtcgcttg aggtcgtacc tgagttcgca ctggtgttga atgatggcaa
    10441 tcggaaagtc acagtggata agcagggtac gttagccggt ctcgagctgt taccgtcacc
    10501 ggttcaacag aaagttcgtg cagcattgca aacaggcagg ctcgagcagt cgtctgcgtt
    10561 ggcccaggtc aagactgagc gaagcacgtt gctcggtatg tcgaatggtg gattaccgtt
    10621 tccaattatc ggcccacttg gcgaggtggt gcggagtgag cgacccacct tccgttggca
    10681 ttcgtccggc ggcgtccaga gttacaccgt aaccgtgact gatgctgatc taaatgtgat
    10741 tgcgacgagt ccgccgctca aaacgactca gtggcggata tcaaaatcgc ttaaagcagg
    10801 tgggatttat gcgtggcagg tgactgcgct gaaagacgac ggtaccagga ttacagcgcc
    10861 cgtgcttccg gccccacagg cgaaattcaa gatcgtcgat cgctcgatag cggagatgtt
    10921 gcagcaagca aagagtacgt atcctgactc gcatctgacg ctcgccgtgc tttacgcgga
    10981 ggcaggtctc atcgacgacg cggagcagga gttacgaatg ctcattcgcg acaacccgca
    11041 ggccggcatc gcgcagaaat tactgcggca agtccaggcg atgagataac acggactttt
    11101 gaggcgcaac tgtaggggcg gccct
//
LOCUS       DASSFQ010000228         5983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1597636, whole genome shotgun sequence.
ACCESSION   DASSFQ010000228 DASSFQ010000000
VERSION     DASSFQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597636"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFH96_08300"
     CDS             <1..590
                     /locus_tag="VFH96_08300"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET6974011.1"
                     /translation="ILFAALFVIAGAVLASGARAEGEVPSPPAIGATIEDFTLPDVDN
                     KDRSLKSLAGKNGTVLLFIAVQCPVSNAYNERMAKLAEDYKAKGIAVIGINANVSEDA
                     AAVKAHAAEHKLSFPILKDPGNKVADRLGASVTPEAYFIDQNNKLLYHGRIDNSRNAA
                     QVESNDLRNALDAALSGKAVEKTEAKAFGCSIKRG"
     gene            598..1149
                     /locus_tag="VFH96_08305"
     CDS             598..1149
                     /locus_tag="VFH96_08305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6974012.1"
                     /translation="MRSTRIVIPVALCILLLGFAAPAQRKKSPAAKSQAPLPVVSAID
                     TDALKGLITQQRERPLLVNFWATFCDPCRDEFPDLVKIDKEYRPHSLEFVTVSLDDID
                     QLKTDVPKFLSSMKATMPAYLLNVTDPEPAINFIDRRWQGDLPATYLYNEKGEVVYKH
                     IGRVNTAELRQAIEKAVKKSDSQ"
     gene            1239..3428
                     /gene="purL"
                     /locus_tag="VFH96_08310"
     CDS             1239..3428
                     /gene="purL"
                     /locus_tag="VFH96_08310"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET6974013.1"
                     /translation="MITPEVIATHNLTQQEFERIKELLGREPSFAELGIFSVMWSEHC
                     SYKSSRIHLKRLPTTGPRVIVPPGENAGVVDLGDGWCAAFKIESHNHPSFIEPFQGAA
                     TGVGGILRDIFTMGARPVASMNSLRFGSLDHPQHGRRNRSLLAGVVAGIAHYGNAFGV
                     ATVGGEVAFDDAYALNPLVNAFALGLVRHDQIFFGKATGIGNPVLYVGAKTGRDGIHG
                     ATMASAEFDEEALEKRPTVQVGDPFLEKLLLEACLEAMRSGAVAGIQDMGAAGLTSSS
                     CEMAARAGTGIELDLSLVPQREQNMTAYEMLLSESQERMLIVAHSGREREVVDIFRKW
                     DLDAVVIGRVRDTGRMQVVHNGEMVADIPVEALTDEAPRYERPMTAATKPEPQITRIE
                     SADLGSELLRLLASGNIASKQWVYRQYDHMVRTNTAILPGADAAVVRIKETRRALAMT
                     LDGNGRYCAANPREGAKLVVAEAARNVVCVGARPIAITNCLNFASPERAEVMWSFSEV
                     IDGMAEACRAFETPVVSGNVSFYNETEGRGILPTPVIGMVGLIEDVRRVVSPGFKKAG
                     DVIALLGTTQDDLGSLVPELDLDRELAVQKACLEAAEAGLLLSAHDCSDGGLAVTLAE
                     SCFSSLGRDAIGAEIDLHSEIGPTALLFSESPSRIVISFDATETNAIQEIAARSNAPF
                     AIIGRVGGTDLKITVDGTEVVAKNVSELEEVWRTSLSSKLQAEVVSG"
     gene            3435..4274
                     /locus_tag="VFH96_08315"
     CDS             3435..4274
                     /locus_tag="VFH96_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET6974014.1"
                     /translation="MHPVIYLNKTMVEATKARVAPVSSAMLYGRGVFTTLAIYRSQPF
                     LWSKHWQRLTTHAKKLDIDFTGCNEKNVGEALLKLISVNQVKEGRARVIMLARHGRDI
                     WKTKEPGAKKTDLLIMTGDPQKPAPTGLSLAVSPYRTNTFSPLSGIKSLNYLDHVLSW
                     EEAQSRDFDEAVVLNERGEIASATTANLFWAKNGTLHTPNLSTGGLAGITRECVIDIA
                     DKHFVPVLEGIYEMTDLTDADEIFLTSSSLGVAAVTTFDFRRYSEGPLTTTIAEAYRQ
                     LNR"
     gene            complement(4307..>5983)
                     /locus_tag="VFH96_08320"
     CDS             complement(4307..>5983)
                     /locus_tag="VFH96_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET6974015.1"
                     /translation="LAAEQWKVEPASVRIVNARFVNHDASKSFTLAEVAKGQKLVKTI
                     PADIAVTAAKDWTVAGTSVPKVDGRDFVTGKHQYTTDLRREGMLYGRIVRPSALEAKL
                     ISSDTKAAEAIPGVVVVRDGDFIGVVAPNRETAIRAEKAIAVHWQAPGQPGNDELFAY
                     LKKNAEAGRARPVGSVADGMTAADKKLSQTYNIAYIAHAPLEPRAAVAEWNDGKLTVW
                     TGTQRPFGVREELAEAFHIPQEKIRVIVPDTGSGYGGKHTGECAIEAARLARASGKPV
                     KLVWTREEEFNWAYFRPAGVIEVNSGVKNDGTVTAWEFHNYNSGGSGIQVKYDFPNQD
                     IQFHNSKSPLRQGSYRGLAATANHFAREVHMDELAQLVNIDPVQFRLKNIKDARLKAV
                     LEAAADKFGWSRKKTDGRGRGIACGFEKGSYIATAAEVSVDAKTGRLKIEHVVESFEC
                     GAIVNQLHLHNQVEGAVVQAIGGALFESIQFKNGQILNGKFSQYRLPRFSDMPSIEIV
                     LLDRKDLPSAGAGETPIVGLAPAVANAIFDLTRVRIRSLPLAPEGTVNRG"
BASE COUNT         1239 a         1677 c         1751 g         1316 t
ORIGIN      
        1 cgattctgtt cgccgccctt tttgtgattg cgggcgctgt tctggcctca ggcgcgcgtg
       61 ctgaaggtga agtgccctcg ccgccggcga ttggcgccac cattgaagac tttacgcttc
      121 ccgacgtcga caacaaggac cgctcactga aatcactggc cggaaagaac ggcacggtgc
      181 tgctgttcat cgcggttcag tgtccggtgt ccaacgcgta caacgagcgc atggccaagc
      241 tcgccgagga ttacaaagcg aaaggcatcg cagtcatcgg catcaacgcg aacgtctcgg
      301 aagatgcggc cgcagttaag gcccacgctg cagaacacaa actctccttc ccgatcctga
      361 aggatccagg caacaaggtt gctgaccggc tcggcgccag cgtcacgcct gaggcttatt
      421 tcatcgatca gaacaacaag cttctgtatc acggccgcat cgacaactcg cgcaacgctg
      481 cgcaagtgga gtcaaacgat ctgcgcaacg cgctcgacgc cgcgctctcg ggcaaggcag
      541 ttgagaaaac agaagctaag gctttcggtt gcagtatcaa acgcgggtaa gtaagtcatg
      601 cgttctacca gaatagtaat tcccgtcgca ctgtgtattt tgttacttgg ttttgcggcg
      661 ccggcccaac ggaagaagag ccccgccgcg aagtcacaag cacctttgcc tgtcgtgtcg
      721 gcgatcgaca cggacgcgct caaaggcttg atcacgcagc agcgtgagcg tccgttgctc
      781 gttaacttct gggccacgtt ttgcgatccg tgtcgtgacg agtttcccga tctcgtgaag
      841 atcgataagg aataccggcc acattcgctc gagtttgtca cggtgtcgct tgacgacata
      901 gatcagctca agaccgatgt gccgaagttt ctcagctcga tgaaggcgac catgcccgcc
      961 tatttattga acgtcacgga ccccgagccc gcgatcaatt tcatcgatcg cagatggcaa
     1021 ggcgatctgc ccgcgacgta tctgtacaac gaaaagggcg aggtcgttta caagcacatc
     1081 ggacgcgtta acaccgccga gctgcgccag gcgatcgaga aagcagttaa gaaaagtgat
     1141 tcacagtaaa actttagcca cggattacac ggattttcac tgtattaatg ctgatctgtg
     1201 ttcatccgtg taatctgtgg caaagatcaa acctcctgat gattactcct gaagttattg
     1261 ccacccacaa cctcacacaa caggaattcg agcgaataaa ggaactactt ggccgtgaac
     1321 cgagttttgc agagctcggg atcttctcgg tcatgtggtc cgagcactgt tcctacaaat
     1381 cttcgcgcat tcatctcaag cgtctgccca ccactggacc acgcgtgatc gtgccccccg
     1441 gcgagaatgc cggcgtcgtc gatctcggcg acggctggtg cgcggctttc aagatcgagt
     1501 cgcacaacca cccgtcgttc atcgagccgt ttcagggcgc tgcaactggt gtcggcggca
     1561 tactgcgcga catcttcacg atgggcgcac gcccggttgc atcgatgaac agcctgcgtt
     1621 tcggatcgct cgatcacccg cagcacggtc gtcgtaatcg ctcgctgctg gccggtgtcg
     1681 tggcgggcat cgcgcattac ggcaacgcgt ttggcgttgc gactgtcggt ggtgaagtag
     1741 cgtttgatga tgcctacgcg ctgaatcctt tggtgaacgc gtttgcactc gggctcgtgc
     1801 gacacgatca gatcttcttc ggcaaagcca ccggcatcgg caacccggtg ctttacgtcg
     1861 gcgcgaaaac cggacgcgat ggaattcacg gcgcgacgat ggcctccgct gagtttgatg
     1921 aagaagccct cgagaaacgt ccgactgtgc aggttggcga tccgtttctc gagaagctct
     1981 tgttggaagc gtgtctcgag gcgatgcgca gtggtgctgt cgcgggaatt caggacatgg
     2041 gtgcggccgg tctgacttca tcgtcgtgtg agatggcggc gcgtgccggc acagggatcg
     2101 agctggacct gtcgctcgtg ccgcaacgcg agcagaacat gactgcttac gagatgctgc
     2161 tgtcagagtc gcaggagcgg atgttgatcg tggcgcattc gggtcgcgag cgcgaggtgg
     2221 tcgatatctt tcgcaagtgg gatttggatg cggttgtgat cggtcgcgtg cgtgacacgg
     2281 gacggatgca ggttgttcac aacggcgaga tggtcgcgga tattccggtc gaggcgttga
     2341 ctgatgaagc gccgcgatac gagcggccga tgaccgcggc gacgaagcca gagccacaga
     2401 ttacacggat tgaaagtgcg gatctggggt cggagttgtt gaggttgttg gcgtccggga
     2461 atattgcgtc gaagcaatgg gtttatcggc agtacgatca tatggtgcgg acgaatacgg
     2521 cgattctgcc gggggcggat gcggctgttg ttcgtattaa ggagactcgg cgtgcactcg
     2581 cgatgacgct tgatggtaat gggcgttatt gcgctgctaa tccgcgtgaa ggtgcgaagc
     2641 tggtcgtggc tgaggcggcg cgcaatgttg tttgcgtagg cgcgcggccg atcgcgatca
     2701 ccaactgttt gaacttcgcg tcgcctgagc gggcggaagt gatgtggtcg ttctcggagg
     2761 tgatcgatgg gatggcggag gcgtgtcgtg cgtttgagac acccgtcgtt tccggaaatg
     2821 tttcgttcta taacgagact gagggccgtg ggattttgcc gacgcctgtg atcggaatgg
     2881 tcgggctgat cgaggatgtg cggcgcgttg tttcaccggg atttaaaaag gcaggcgacg
     2941 tgattgcgct gctcggtacg acgcaagacg atctgggcag cctcgtgcct gagcttgatc
     3001 tcgatcgtga gttggcagtg caaaaggctt gtctcgaggc ggctgaggcg gggttgttgc
     3061 tctccgcgca tgattgttcg gatggcggac tggccgtcac gcttgccgag tcatgtttct
     3121 cgtcactcgg gcgtgacgcg atcggcgctg aaatcgatct ccacagtgag attggtccaa
     3181 cggcgctgtt gttcagcgag tcgccatcgc ggatcgtgat cagtttcgac gcaacggaga
     3241 cgaatgcgat tcaggagatc gccgctcgca gcaatgcgcc attcgcgatc atcggtcgcg
     3301 ttggtggtac cgatttgaaa attactgtcg acggcactga agtcgtggct aaaaatgttt
     3361 ctgagttgga agaggtttgg cgaacgtcac tatcgagcaa actccaggct gaagtcgtga
     3421 gcggttagca tttaatgcat cccgtcatct acctcaacaa aaccatggtt gaggcgacga
     3481 aagcgcgcgt cgcgccggtc tcttccgcga tgctctatgg gcgcggcgtc ttcaccaccc
     3541 tcgccatcta caggtcacaa ccattcctct ggtccaaaca ttggcaacga ctcaccacac
     3601 acgctaaaaa actcgacatc gatttcaccg gctgtaacga gaagaatgtc ggcgaggcgc
     3661 tgctaaaact gatctcggtg aatcaggtga aggaaggacg cgcgcgcgtg atcatgctcg
     3721 cgcggcatgg tcgcgatatc tggaagacga aagagcccgg cgcgaagaag accgatctgc
     3781 tgatcatgac gggcgatccg cagaagcctg cgcctacggg cttgtcgctc gcagtctcac
     3841 cttatcgcac caacacgttc tcgcccctct cgggaatcaa gagtttgaac tatctcgatc
     3901 acgtgctttc gtgggaggaa gcgcagagcc gcgacttcga tgaagcagtc gtgttgaatg
     3961 agcgtggcga gatcgcttca gcgacgacgg caaacttgtt ctgggcgaag aatggcacgc
     4021 tgcacacgcc gaatttgagc acgggcgggc tcgcgggcat cacgcgcgaa tgcgtgatcg
     4081 atatcgctga caagcatttc gttccggtgc tcgaggggat ttacgagatg actgatctga
     4141 ccgacgcgga tgagatcttt ttgacgtcgt cgagtttggg tgtggcggca gtgacgacat
     4201 tcgactttag acgttattca gagggaccac tcaccaccac gatcgccgaa gcctatcgac
     4261 aactaaaccg ctgatgacgc ggataaaaat ccggatttca tccgtgttat ccgcggttaa
     4321 ctgttccctc gggtgcgagc ggcaacgatc taattcgaac acgtgtcagg tcgaagatcg
     4381 cgttcgctac ggctggcgcg aggccgacga tcggcgtttc acctgcaccc gctgacggca
     4441 gatcttttcg atcgagtaga acgatctcga tcgacggcat gtcactaaac cgcggcaatc
     4501 gatactgcga gaatttcccg ttaagaatct gcccgttctt gaactgaata ctctcaaaca
     4561 acgcgccgcc aatcgcctgc accaccgcgc cttcaacctg gttgtgcaag tgcaactggt
     4621 tgacgatcgc gccacactcg aaactctcga ccacgtgctc aatcttcagg cgcccggttt
     4681 tcgcatcgac tgaaacttcc gccgcggtcg cgatgtaact gcccttctca aacccgcacg
     4741 caataccgcg cccacgtccg tcagttttct tgcgactcca tccaaactta tcagccgccg
     4801 cttccaacac agccttcaag cgcgcgtcct tgatgttctt caaacgaaac tgcaccggat
     4861 cgatattgac gagttgggcc agctcgtcca tgtgcacttc acgcgcgaag tggttcgcag
     4921 tcgccgcgag cccacgatac gagccctgtc gcagcggcga cttgctgttg tgaaattgaa
     4981 tgtcctggtt cggaaagtcg tacttgacct gaatacccga gccgccggag ttgtagttgt
     5041 gaaactccca cgcagtgaca gtgccgtcgt tcttcacccc actattcact tcgatcacac
     5101 ccgctggacg aaagtaggcc cagttgaatt cttcttcgcg cgtccagacg agcttgaccg
     5161 gcttgccact cgcgcgtgcg agccgcgccg cttcgatcgc gcattcgccc gtgtgcttgc
     5221 caccgtaacc agaacccgta tcaggcacaa tcacacgtat cttctcttgc ggaatgtgaa
     5281 acgcttcggc cagttcctcg cggacgccga acggccgctg cgtgccggtc caaactgtca
     5341 gcttgccatc gttccattcg gcaacagcgg cccgtggctc gagtggcgcg tgcgcgatgt
     5401 aagcgatgtt gtacgtttgc gagagtttct tgtccgcggc ggtcataccg tcagcgaccg
     5461 atccaaccgg acgcgcgcga ccagcctcgg cgttcttctt caaataagcg aacaattcgt
     5521 cgttgcccgg ttgtcccggc gcctgccaat ggaccgcgat cgctttttcc gcacgtatcg
     5581 cagtttcacg attcggcgcc acgacaccga tgaagtcgcc atcgcgcacg accacgacac
     5641 ccggaattgc ctcagccgct ttggtatcgc tcgagatgag cttcgcttcg agcgctgatg
     5701 gtctaacgat gcgaccgtaa agcatgcctt cgcgtcgcag gtcagtcgtg tactgatgtt
     5761 ttccggtgac gaaatcgcga ccatcaacct tgggaacaga cgtaccggcc acggtccagt
     5821 ctttcgctgc ggtaaccgcg atatcggcag gaatagtctt gacgagtttt tgacctttcg
     5881 cgacttcagc gagcgtaaaa gattttgacg cgtcgtgatt gacgaaacgc gcattcacga
     5941 tgcgaaccga cgcaggctcg actttccact gctcggcagc cag
//
LOCUS       DASSFQ010000229         3389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1884952, whole genome shotgun sequence.
ACCESSION   DASSFQ010000229 DASSFQ010000000
VERSION     DASSFQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884952"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFH96_08325"
     CDS             complement(<1..244)
                     /locus_tag="VFH96_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974016.1"
                     /translation="MSDPNTPATGAEMSTAETLTGIFFEPSRTFEALRVRPRFLVAGL
                     IAFILTCLVTAALYARVDMAQFIRDRIEQSPRAAQIS"
     gene            complement(362..1369)
                     /locus_tag="VFH96_08330"
     CDS             complement(362..1369)
                     /locus_tag="VFH96_08330"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET6974017.1"
                     /translation="MSARVEIDQTNVSVPAAETRETLVSVSGLVKHFPVEGSTDVVRA
                     VDGVSFEIFAGETLGLVGESGCGKSTVGRCLLRLIEPTAGHIYFEGRDILALNRKELR
                     EIRREMQIIFQDPYASLNPRMKIGDIVGEPLVIHNQGTKSERRDRVAALLRRVGLDPD
                     YMNRYAHEFSGGQRQRIGVARALALNPKLIVADEPVSALDVSVQAQVVNLLQELQEEF
                     KLTYLFISHGLAVVEHISTRVAVMYLGRIVEIAEAGELYANPLHPYTQALLSAIPIPD
                     PKQKRKRIVLEGDVPTPINPPSGCRFRTRCPIAIEDCARIDPELREVSPGHHVACIRV
                     P"
     gene            complement(1353..2378)
                     /locus_tag="VFH96_08335"
     CDS             complement(1353..2378)
                     /locus_tag="VFH96_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974018.1"
                     /translation="MAHLLEVRNLQTHFSTRTGLVRAVDGVSFHLDRGELLGLVGESG
                     CGKSMTALSVMRLIAPPGKIVNGEILFDGKDLLKLSDSEMREMRGDDIAMIFQDPMTS
                     LNPVFTVGEQIAEALRLHRKLSRKQARQATIEAMREVAIPDPARRVDDYPHQLSGGMR
                     QRVMIAMALACNPKLLIADEPTTALDVTIQAQILELLDELRKQRELAVLLITHDLGVV
                     AEVADRVAVMYTGRIVEESPVDELFARPKHPYTEGLLRSVPKLTGTSVIQKERLETIE
                     GVVPRPTDLPPGCHFAPRCPHRMPRCTAEEIPLYQLEGLVEVRCVLYDLAAAVAADHA
                     EVHERPS"
     gene            complement(2385..3272)
                     /locus_tag="VFH96_08340"
     CDS             complement(2385..3272)
                     /locus_tag="VFH96_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET6974019.1"
                     /translation="MVHLNTLKRIAFLSGALLLLSVDALAQSGGMAPPTAAWPRYRVA
                     GEEFSIALPTLPAMTTYAQTREGREPRTQRYLGVYADGVVYTIYCDDTEPQDAVKNSR
                     KRLLPMQGWEPSSEQDVNRDGATGKQFTSSNPLGGVLQVLATKKHFYRIQAFGATVAD
                     PRVQHFFSSLMLGKNPDGIEVTDGPGTPFEPVDVQIPADSMSPGNQVDRKVVLLMKTE
                     PTYTEEARQHRTTGAVILKAVFSANGSVVNIQVKSGLPNGLTEQAIEVAKRIKFIPAA
                     KDGKFVSVWTQLEYNFNLF"
BASE COUNT          770 a          988 c          895 g          736 t
ORIGIN      
        1 cagagatttg ggccgcgcgc ggtgattgct caatccggtc gcgaatgaac tgggccatat
       61 caactctcgc gtacaaggcg gcagttacga ggcaggtcag gatgaacgcg ataagaccag
      121 ccacgagaaa gcgaggccgt acgcgcaggg cctcaaacgt tcttgacggc tcgaagaaga
      181 tgcccgtcag ggtttccgcc gttgacattt ccgcgccagt cgcaggcgtg ttcggatcgc
      241 tcattagatt cctcctaagg aagattacgg gtaagaaagc tgtctactga atacgcagat
      301 tacaccgata aagttatcaa acaatctgca tgatctgtgt aatctgtgga taaaaactct
      361 gttacggcac acggatgcac gcgacgtgat gaccgggcga cacttctctc aactccggat
      421 cgatgcgtgc gcaatcttca attgcaatcg gacaacgcgt ccgaaagcga caacctgacg
      481 gcggattgat cggcgtcggc acatcaccct ccaacacgat ccgctttcgc ttctgcttcg
      541 gatcaggaat cggtatcgct gacagtagcg cctgcgtgta cgggtgcaac gggttcgcat
      601 acaactcgcc ggcctcggct atctcaacaa tgcggcccag atacatcacc gccacacgcg
      661 tcgagatgtg ttcgaccacg gcgagcccgt gcgagatgaa caggtaagtc agcttaaact
      721 cttcctgtaa ctcctgtaac aggttcacca cctgcgcctg caccgaaacg tccagcgccg
      781 acacgggctc atcagcaacg atcagcttcg gattcagcgc gagggcgcgc gcgacgccga
      841 ttcgttgtcg ctgcccgcct gagaattcgt gggcgtagcg attcatgtaa tcaggatcga
      901 gccctactct tcgcagtagc gcagcaacgc gatcgcgtcg ctcactcttc gtgccctgat
      961 tgtgaatcac aaggggctcg ccgacgatgt cgccaatctt catgcgcgga tttagcgacg
     1021 cgtaaggatc ctgaaagatg atctgcatct cgcgacgaat ctcgcgcagc tctttccggt
     1081 tgagcgcgag aatgtcacgt ccttcaaaat agatgtgacc cgcagtcggc tcgatcaagc
     1141 gcagcagaca acgtccgact gtcgacttgc cgcagcctga ttcgcccacc agtcccagcg
     1201 tctctccggc aaagatctca aacgacacac catcaaccgc gcgcaccaca tccgtcgagc
     1261 cttcgacagg aaagtgtttg acgagcccgc taacgctgac gagcgtttcg cgtgtttccg
     1321 ccgctggtac tgaaacatta gtttgatcta tttcaactcg ggcgctcatg aacctccgcg
     1381 tgatcggccg ccactgcagc cgcaagatcg taaagcacgc aacgcacttc tacgaggccc
     1441 tcgagttgat acaacggaat ctcttctgcc gtgcaacgag gcatacgatg cggacagcgc
     1501 ggcgcgaagt gacaaccggg cggcagatcc gtcggtcgcg gcacgacgcc ttcgatcgtc
     1561 tcgagccgtt ctttctgtat cacagaagtg ccggtgagtt ttggaacaga acgcagcaag
     1621 ccttccgtgt agggatgctt cggccgcgca aacaactcgt caaccggcga ctcttccacg
     1681 attctgcccg tgtacatcac cgcgacgcga tcggcgactt cagccaccac accgagatcg
     1741 tgagtgatca agagcacagc cagctcgcgc tgttttctca attcatcgag cagctcgagg
     1801 atctgcgcct ggatcgtgac gtcgagagcg gtggtcggct cgtcagcgat cagaagtttc
     1861 ggattgcagg cgagggccat cgcgatcatc acgcgttgtc gcatgccgcc ggaaagctgg
     1921 tgtgggtaat catcaacgcg acgagcagga tccggaatcg cgacttcgcg catcgcctcg
     1981 atcgtcgctt gtcgtgcttg tttgcgcgag agcttgcggt gtagtcgcag ggcttcggcg
     2041 atctgttcgc cgacagtaaa aacaggattg agcgacgtca tcggatcctg aaagatcatg
     2101 gcgatatcgt cgccgcgcat ctcgcgcatt tcggagtcgg aaagtttgag tagatccttg
     2161 ccgtcaaaaa gtatctcgcc gtttacgatc tttcctgggg gcgcaatgag tcgcatgacg
     2221 gaaagcgcag tcatcgactt cccacaaccc gactcaccaa cgagtcccag caactcgcca
     2281 cgatcgagat gaaaactcac cccgtcaacc gcacgtacga gccccgtgcg tgtggaaaag
     2341 tgtgtttgaa gatttcgaac ttcgagcagg tgggccatta gtcgtcagaa gagattgaag
     2401 ttgtattcga gctgcgtcca cacagagaca aacttgccgt cttttgctgc gggtatgaat
     2461 ttaatccttt ttgcgacttc aatggcttgc tccgttaacc cattcggcaa acctgacttg
     2521 acctggatat tcacgacgct gccattggca gaaaacacgg cctttaatat aacggccccg
     2581 gtagtccggt gttgacgcgc ctcttccgta taagtcggct cggtcttcat cagcagcacg
     2641 accttccggt cgacctgatt acccggggac atactgtcag cgggaatctg aacatcgacg
     2701 ggttcaaacg gagttcctgg accatcagta acctcgatgc catcgggatt ttttcccagc
     2761 atcaacgagg aaaagaagtg ttgcacacgc gggtcggcta ccgttgcacc gaaagcctga
     2821 atccgataga agtgcttctt agtggccagg acctggagca ctcctcccag cggatttgaa
     2881 gacgtgaact gtttacccgt agcaccatca cgattcacat cctgttcact cgagggttcc
     2941 cagccctgca tgggcagaag ccgttttctg gaattcttaa ccgcgtcctg tggctcagtg
     3001 tcgtcacaat agatcgtgta caccacgccg tcagcataga cacccagata tcgctgcgtt
     3061 cgcggctccc tgccttctcg agtttgagca taggtcgtca tcgccggaag ggttggcaaa
     3121 gcaatggaaa actcttctcc cgcaactcga taccttggcc acgcagccgt tggtggcgcc
     3181 attccgccag actgcgccag cgcgtccact gacaaaagca atagcgcacc agacaagaac
     3241 gcaattcgct tcaatgtatt cagatggacc atcagtcgtt agtaaaaatt aaagttgtac
     3301 tcaagttgca tccacgtcga gaccagcttg ccgtctttca tggcgggaat aaactttatc
     3361 ttcttcgcgg cctcgatcgc attttcttc
//
LOCUS       DASSFQ010000230        25043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1139773, whole genome shotgun sequence.
ACCESSION   DASSFQ010000230 DASSFQ010000000
VERSION     DASSFQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 25043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25043
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139773"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFH96_08345"
     CDS             complement(<1..277)
                     /locus_tag="VFH96_08345"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET6974020.1"
                     /translation="MSQHLFLYGTLLPGEAPKEIASIVKRFRRLGSAHVRGRLYDFGE
                     FPGAVLDPSSRTMIHGELVVLPSDERILEALDRYEEFDPMDPKKSLFI"
     gene            complement(274..720)
                     /locus_tag="VFH96_08350"
     CDS             complement(274..720)
                     /locus_tag="VFH96_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974021.1"
                     /translation="MTVKERVEIVYLIAGTISAVGTVVAAVFAAFVYQRNSTFERARW
                     ASSLYEKFYEGVGLKQIRDKLDCLNDSDSVNEIVIREEPAFTDYLNFFEFLAFLRTSK
                     QLKDSEIDVLFGYYLDCLNRHDRVKTYISNPENGYEGLAKLLASRI"
     gene            1106..4210
                     /locus_tag="VFH96_08355"
     CDS             1106..4210
                     /locus_tag="VFH96_08355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6974022.1"
                     /translation="MIPSALGFCFIVSALFGSNLTEFVVVLIVAQVISSVVVWKSWRG
                     TQEQLAGVRHELSSLQGIVDVSRDAIIGVTTEGVIMSWNRGARGIYGYTSKEALGAPI
                     SMLFDHRRGQEASLLFEKVTRGENVTQHEMVHLKKGRTPIDVSLTICPIMEGKTMTGA
                     SVVARDITERKRAAGSLAQQAAAMKASMDGMAIIDHTGVCIYLNDAYAKTFGHADPTR
                     LIGASWEMFYFEEELARMKEQIMPAVWRDGSWRGEAMGLKLNGGTFPLEISISSVDGG
                     GLVQVVRDITERKNLEETLRNSSLKDDLTGLFNRRGLLKQAAPYFDFARRQKEKLLLL
                     FIDLDGMKKINDEFGHNEGDNALIQTAAILNHSFRSSDIIARLGGDEFTVLVTDLNAS
                     KEEAIARLNENLRAYNASETRNHKLSFSIGVATLEPERMACFEELLEQADQAMYEQKR
                     SKRRRTSERSQNEAQYAPDSIPFALPTNSVGRQGTFDNAAIGMAVVSVDGSWLQVNES
                     LCKLLGYSEQELRATSFQRLTHTEDVRHVQSHIQRVLEGYIHSHEQEKRYIHEQGHTV
                     WVQWHVSLLKDSESGAKRLFFQVQDISDRKKAEEKLTQDTLTGLPNRARFYDLLKLRV
                     SNKTRQCAVLLLDVDRFKLVNDSLGNASADQLLIQIAQRVKTCMRQGDVLARVGGDEF
                     AVLLDDVSGEEEASSVATRIQQALSISFNLLGQEVYTTMSIGIALASDYEQVSDMLRD
                     AETAMHQAKARGKARYEVFGHDMHGELMSRLKMETDLRHACERDELFVDYQPIVSLGS
                     RTLIGFEALVRWRHPEFGIIPPQDFIPVAEETGQILTIGQTVLESACRQAREWQQTYP
                     VAPPLFVSVNLSVKQFNQPGLVENIAGLLERFQLPPRCLKLEITESVFTDNIEAAVGL
                     LTQLRELGVQLSIDDFGTGYSSLSYLQRFPIDTLKIDRSFVTQMMENEENLAIVRTIV
                     ALAQNLGMDVVAEGVETEDQLKLLRKLECENGQGYLFSTPLGGWQLNEFIADYVDAPT
                     ALVA"
     gene            complement(4318..5487)
                     /locus_tag="VFH96_08360"
     CDS             complement(4318..5487)
                     /locus_tag="VFH96_08360"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET6974023.1"
                     /translation="MIQPGERVLAIISDKTRDDNTHELFPIANEFLTKRGVASFDALV
                     AQGTHPPMSAAQKFSKIGNADFRGSLFDHRWDDPDELITLGELSAEAVGELTGGLIDN
                     AVPVSINKLLAPGIYDTVLVFGATVPHEVAGFAGGAKYFFPGVAGPELTHTTHWLGAL
                     AGIENIIGQVETPTRRLIEAAADLISARIISLNTVVSRNNGELVTYALFTGDIREAFR
                     RAAEVSRQVHIRYTGRKYKRVIALLDQHYDELWVGGKASYKLGAIIEEGGELIIYAPH
                     LTKLSETHGELIEKYGYAPLESVRDMLGVSQELRENLCIAAHLAHVAYAGRLDQHGKV
                     VPRYQITMATGLDEETCRRVNLGYLDYRTLDYEAMRSDPDTLIVADAGRDLYQVS"
     gene            complement(5602..7161)
                     /locus_tag="VFH96_08365"
     CDS             complement(5602..7161)
                     /locus_tag="VFH96_08365"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HET6974024.1"
                     /translation="MSKLETPISEYAIVVGPNDNVAVVKKETFVDLEVLLPNSALVKL
                     RDAVPPGHRFATRDIPAGEFVTQFGQPIGTSLGIQSGEQITHANMSNDVPLIRDLPDS
                     LHTPPPDYIPEAERRTFMGFRRGDGRVGTRNYVLIVPTSMCASHEAQQISMIAEFTLY
                     NRDKYPNVDGVVAIPHNKGCGCQDGSTIDVMLRTLSNYADHPNVGGVILIDLGCEKTN
                     LSQVEKYLLKREKSFDKPVAKIGIQEVGGTQAAIQQGLKHVEQMLSEVNRAAREECSV
                     SEIVLGVKCGGSDGFSGISANPSLGRAADLLVRSGGTVLITEVPEFCGAEHLLACRAK
                     DVETGREVYRMVDWYKEYASKFGAVLAQNPSPGNITGGLLNITIKSLGAIAKAGTTRV
                     EDVLEYAESWRTRGLNLMQGPGYDQESTPGLVAAGATVVVFTTGRGTTIGNAIAPVIK
                     LASNTPIFERMKNDLDLSAGGVIDGTETIDEVGGRVFDHVIEVASGLEAKAETHKHRE
                     FQFWAEQTVSL"
     gene            complement(7228..7875)
                     /gene="eda"
                     /locus_tag="VFH96_08370"
     CDS             complement(7228..7875)
                     /gene="eda"
                     /locus_tag="VFH96_08370"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET6974025.1"
                     /translation="MKTDVIKHIRDTGVIPVVRATTADEAMRAIDAIREGGISVLEIT
                     MTVPGAVKVIEDVAKRYGNDALVGAGTVLDAETASACVSSGAQFIVSPALNLETIARC
                     RELDIAVMPGALTPTEVVQAWNAGADFVKVFPAGAVGGPSYLKSLKAPLPQIELVPTG
                     GVSLKTAADFIRAGAAALGVGADLVDIKAIREGQSALITERAKQFIEIVREARAS"
     gene            complement(7900..9000)
                     /locus_tag="VFH96_08375"
     CDS             complement(7900..9000)
                     /locus_tag="VFH96_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET6974026.1"
                     /translation="MSAFVAKSKSGCRWDIVSLGEVMLRLDPGEGRVATTRQFQVWEG
                     GGEYNVARGLKRCFGMDAAVVTALADNAVGRLVQDLIYQGGVDQSHLKWVKFDGVGRA
                     VRNGLNFTERGFGVRAAAGCSDRGHTAISQLRPGDIDWEQVFGVEGARWFHTGGIFCA
                     LSETTPAVAREAMEVARKHGTIISYDLNYRESLWKSIGGQARAIAVNRELAPLVDVMI
                     GNEEDFTAALGFEVEGQDEHHSKLDVAGFHRMIQRAVAEFPNFKVVATTLRNAKTATI
                     NDWGAVCYTGGELYQAPVRENLEILDRVGGGDSFASGLIYGFLTERGPQWAVECGAAH
                     GALAMTTPGDTTMATLSEVERVMKGGTARVAR"
     gene            complement(9100..10386)
                     /locus_tag="VFH96_08380"
     CDS             complement(9100..10386)
                     /locus_tag="VFH96_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6974027.1"
                     /translation="MIEEQVSIRVENQRSSAFGFRWVICALLFFAATINYIDRQVIGI
                     LKTTIHDEIGWSEVDYSWVVFSFQGAYAIGLLGVGRLIDRIGTRKGFSLAIIFWSVAA
                     MAHALAHTVLGFGAARFFLGLGEAGNFPAAIKTVAEWFPKKERALATGIFNSGTNIGA
                     LVTPLVVPMITIAWGWRAAFIATGAIGFIWLVAWLLLYRRPEEHTSLSKAELEYIQSD
                     PPDPVVNIPWGRLLPHRQTWAFSVAKFMTDPIWWVYLFWLPDFLHKRHGLSLKDFGPP
                     LVVVYLMADIGSIGGGWLSSSLIKRGWTINAGRKLAMLICAIAVVPIVFAAQTSNLWV
                     AVLLIGLAAAAHQGWSCNLFTTTSDMFPRRAVGSVVGIGGMAGAVGGMMIARLVGEIL
                     ERTGSYVPIFIIAASAYLVALLLFHLLSPRLDPANL"
     gene            complement(10383..11147)
                     /gene="kduD"
                     /locus_tag="VFH96_08385"
     CDS             complement(10383..11147)
                     /gene="kduD"
                     /locus_tag="VFH96_08385"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HET6974028.1"
                     /translation="MILDQFKLHERAALVTGASTGLGAAIAIALAEAGAQVACHGNTR
                     SPESTCDQIESAGGVAHPITGDLSKAETAQDLIAQTLDRFGRIDILINNAGTIRRAPA
                     VDYSDDDWTAVIEVNLSSVFRLSKLAGKQMIDSGRGGKIVNIASLLSFQGGITVPAYA
                     ASKGGVAQLTKALANEWAKHRINVNAIAPGYMRTTNTAALQADETRNRQILERIPAGR
                     WGEPEDVAGAAVFLSSRASDYVNGHVLVVDGGWMGR"
     gene            complement(11144..11521)
                     /locus_tag="VFH96_08390"
     CDS             complement(11144..11521)
                     /locus_tag="VFH96_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6974029.1"
                     /translation="MSTTTSENHGNGNGNGFPRHLNWTTIPVEHPAEGIERQMFVGNR
                     MMICRFRFKPFLVTPEHDHPHEQMTIVERGRVRFFIEGKEQIASAGDVLHFPSNCWHG
                     ATMMDEEVVLIDIFSPLREDFLK"
     gene            complement(11534..12373)
                     /locus_tag="VFH96_08395"
     CDS             complement(11534..12373)
                     /locus_tag="VFH96_08395"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET6974030.1"
                     /translation="MDQEVNQNPITAETCFVPKTHEGKGRRTAVAPGETAARFLHYGR
                     ITLAAGDPPLQFNNNDHEVGLICLNGKVTVRAEGETFDLDRYDAVYVPRDSEIEVSTT
                     DGGDLAEVSAPVAKRYPLKFVSYKEVRQNPKLHLIAGKPPAERDLNVLIGANVEAGRI
                     MAGVTFSTPGNWTSWPPHEHSKLLEEAYLFIDMPSPSFGVQFVYTDPNKPELVQVVRE
                     GDCVLMPQGYHPNVAAPGGQINFLWMMAAIREGEDRLYGVVNVQPEYAAGGSGLEAVS
                     DKK"
     gene            complement(12465..14309)
                     /locus_tag="VFH96_08400"
     CDS             complement(12465..14309)
                     /locus_tag="VFH96_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974031.1"
                     /translation="MIRGFKSVLICVICVSAVITAQGSTVTTASLRDDVNTFLGKELA
                     AHLSAIKSLDPPPDQILGVPTTGEYSWGTFMRSLAAYAETTGNRELAGRDIAKWVGQI
                     GLIESRAGSKAFSQLYAALALRHFGRDLKTNALWQSLSPEEQKAWRSLLNPERFYDPK
                     TNRVINLAENYLGVAARIASIGYELGLSNDRAFLDKLLDRAAVQFTEGNLYADDAPPN
                     GRFDRYSNEYARYIWEAAESAGRKDLLDKVRPSLMVQMRLWWDVVSEDGYGYNWGRSQ
                     GVVSYLDTPEIAGFLASHPEFRPAPLADIAALYYQAWNYLLHDYRSDTHLLSIFAFGR
                     GNYAYISRDREWQQTTGFFGKLANSHTPIVNALEKEGIATFPVVPSLRDVARFEFFRK
                     SPERSAGVWVVRQGALRFALPITTGPKPGMSDYLPAPHGLPGFAAPVEQIYPVLTPYV
                     ELADGRTVVATDGADVIEPASDGRSLRVRWNKWAVVGTPSGKVQDVGLVSEVVWAIEN
                     GTLTREETLSSKGGVTIRRWRIAVPSTYDQVETEMIKGVRVDRFYTAKASLDVRMIDA
                     NFPLKTSIVASGNSALGRGVHGAIPLHLVFEAHDVPAPLKYKIALTPR"
     gene            complement(14325..17369)
                     /locus_tag="VFH96_08405"
     CDS             complement(14325..17369)
                     /locus_tag="VFH96_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HET6974032.1"
                     /translation="MEVSTAAGETVNTTSGEVARVVDKRQVQNLALNGRNYMQLVTLI
                     PGAAILDEDQLALTTSLSISQAAINGNRPNYNSLSVDGGSNMDSGSNNSQVNNVGIDF
                     IQEVKIQTSNFSAEYGRNAGASVNVVTRSGGNDYHGSAYEFGRNDKLDSRNYFSPKKG
                     KLRFNNFGWNFNGPIVKDKLFFFVGQEYKYIRQDAAPVSRNLPTRAERLGDFSLRTGA
                     LNVPVGYTAINPANGATVAAGQPIPGRNLANLRLNGAPVGVTPNGAAIAAVYTAMEKI
                     AVAYSDTPTASNATFQQPNPFDYREDNIRIDYRINSKHSVYGRYLHDKYDLIDPFGTF
                     INSQLPTVPTNRLRPGSSYQLSYTWLITPTLINEAKANASWNGQRIPPVGEFWKRSTF
                     GFTYPQLSSGGRFDDGIPNVTFAGNGAMANFNGPNGSLLSPTADIAVSDNITIIRGKH
                     SLKTGVLVVRNRKDQNGRSIYTGNVAFNSGAASGAVTTGNAFADALLGNFRTYSEADN
                     DPIGFFRFNQVEAFVSDSWKITRDLSFEIGARFYHFGPTYTQANNMANFDPSHYDPSK
                     AVTVLSNGTIDLTKGGNRFNGLVRAGDGIPIDERGRVATSDAALALVPAGAPRGFYQP
                     ADRIAPRFGFAYAPFHDDKTSIRGGFGMYYDKVEGNLIFSQVNAPPFVSTPSLSNGNL
                     ANPAGGTPANAALIGSINSIDPNLDVAYSMNFSLGVQRELPRGFFIEANYVGNLGRHL
                     IRQPDLNQVSFKALTANLPANGGPNVNTDALRPFKGFTQILYRLSDANSNYNGMQLYA
                     AKRKGNLELTASYTWSKALTDTSGNGDGVDVGEDPFNRHSNYGLATFDRRHIFVTTYD
                     YLLPWFSKMKGVGGAVLSGWEISGITRYQSGPYLTVTANTAIGTRRADYVGGPVRAAN
                     FAPNNQWINDAAFAPAPATRRGNSGVGIVQGPNLQTWDFSMRKQFTLSERFNLRFQAD
                     MFNAFNRANFRSPATVLTTGGFGRITTAGPARNIQFGLKLNF"
     gene            complement(17799..19013)
                     /locus_tag="VFH96_08410"
     CDS             complement(17799..19013)
                     /locus_tag="VFH96_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6974033.1"
                     /translation="MRKINPHDFTVARRGTSREINRQIALNLIRTHQPVSRADLARLM
                     NVRRGVASLLVSELLSENLIFEGALGESVGRGRRPTFLYIDSRERCVVGVDIRASRTY
                     ILVTDLVGRQLGAVSSFQTNRDVDTLIQELARRIKQILADYNGCAGVGVSVPGMVEPT
                     KGRVVHAPTLAWHDVNLRDPLAAALGIPVQIENSGKACALAQLWATRSDVVASGNSVF
                     VSVSDGVGVGIIVNGEVLRGRHNTAGEFGHVPLSIDGPKCSCGATGCWEAYVSNLATL
                     SRYFGRDLSDPRLRDSEEAGFSVEDLIARARAGEAKALAALNSTARYLGLGLASIVNA
                     LDPARVYIGGEITTAWSLIEPIARSALRERTLTEFGGATEIHVVPAEDHPRLRGAVAL
                     VAAPAFAAPMVA"
     gene            complement(19220..20221)
                     /locus_tag="VFH96_08415"
     CDS             complement(19220..20221)
                     /locus_tag="VFH96_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6974034.1"
                     /translation="MPVTLEDIARALNVSKMTVSRAINNHPEISSETRARILATAQKM
                     NYRPNQFARALMTKQSYLIGIVVPDLMHSYFAEICRGVEAHARPVGYQNLICSTDEEP
                     RKEMDEIEALLSRTDGLIVASALSASEVKFYKRLISDGAKIVLIDRVLEGLRCSAVTT
                     DDVQVGRLATEHLIKLGHRKIGHLRGPDVSTALKRLQGYREAMSKARLKALVHDCGFT
                     ETDGYAAMQKLIASGELPPAIFAANDPAAIGGMAAANEAGLKVPDDIAFVGAGSIHYG
                     DMLRVPLTTVSWSKAEMGQTAARLLLELIDGKKKNRTITIPPELVIRQSSGNADSRG"
     gene            complement(20228..21001)
                     /locus_tag="VFH96_08420"
     CDS             complement(20228..21001)
                     /locus_tag="VFH96_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6974035.1"
                     /translation="MIWAVLVFSSVVTVGQAPVATPSPDCAETKTRLDRAEARLKDWP
                     ALERYGEENIKTVPASMDGPRRVVFMGDSITDSWDDPKYGGFFPGKSYINRGISGQTT
                     PQMLIRFRPDVIALKPKVVVILAGTNDLAGNTGPTTLAAIEDNLISMAELATANGIRV
                     VFASVLPVSDYEMRDGKPIVQTVRRPPAQILELNKWLKEYAARHHHTYLDYFSAMVDD
                     KGFLKNELSDDGLHPNAQGYVVMAPLAEAAITASLKRRK"
     gene            complement(21097..22050)
                     /locus_tag="VFH96_08425"
     CDS             complement(21097..22050)
                     /locus_tag="VFH96_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974036.1"
                     /translation="MVDLKQNLNRLGPVLGLLLVIGVFSLLVDTPGRLLSPNNLRIVL
                     SQTVIVAIGAIGMTLIIISGGIDLAVGSTIALTGVVTALAINAGLPPAVALLSGILVG
                     GFVGVINGVVITRLRVVPFIATLGMLGVARGVAKWLANQQTVNMPGTWLNELLVMFPK
                     PSWLLVAPGVWITIILAVCAAIVLRNTVFGRRVFALGSNEAAARACGIATDRLKIWIY
                     GIAGLLFGLAGVMQMSRLRQGDPTVAAGSELDIIAAVVIGGGSLSGGEGSILGSMIGA
                     LIMAFLRNGCQQVGWPNYIQEIIIGAIIVVAVAIDRWRSRS"
     gene            complement(22109..23110)
                     /locus_tag="VFH96_08430"
     CDS             complement(22109..23110)
                     /locus_tag="VFH96_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6974037.1"
                     /translation="MRKLLLLLLALTFAIACTPHGGPSTNNAKKKLTIAVIPKGTTHE
                     FWKSIHAGSNKAASELTAQGTEVEVIWKGPLREDDREQQIQVVEGFAAQGVSGIVLAP
                     LDNRALVRPVADAERAGVPTVIIDSGLESDEFVSFVATDNRKGGTLAADRMGQLLNGK
                     GKVLVLRYAEGSASTTEREEGFISQMKAKFPEIELVSTDQYAGATRDTAKRASENLLN
                     RFGDVVQGIFTPNESSTAGMLLALQDIGKAGKVLFVGFDSSQTFIDAINANQLHGIVV
                     QNPFNMGYLGVRTMVDSLLGKPVEKKIDTGVMLVTKENLQSPEVNALLHPPLDQYLK"
     gene            complement(23123..24061)
                     /locus_tag="VFH96_08435"
     CDS             complement(23123..24061)
                     /locus_tag="VFH96_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6974038.1"
                     /translation="MQIISFATQETKWSVPRLGMILEVDGQDTGYRLDCEKLFPEAER
                     PSHPLAWFDMDGPWFQKARATHDILIKDKQALAEARDKGWLVPSRDAYWFAPVPRPGK
                     IICIGLNYRDHAAESKMPIPEKPVVFSKFSTAVIAPGEPVVLPSTSQQVDYEAELAVV
                     IGRHAKHVKADRAYDYVCGYTAFNDVSARDFQFGDGQWQRGKSCDTFAPMGQTIVTTD
                     AIPDPHKLSIKLVLNGQTMQDSNTDQLIFGIPKLIEFLTESITLEPGDVIATGTPPGV
                     GFARNPPVFLKPGDRMEVLIEGMGGLGNPVVSEENS"
     gene            complement(24062..24640)
                     /locus_tag="VFH96_08440"
     CDS             complement(24062..24640)
                     /locus_tag="VFH96_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET6974039.1"
                     /translation="MNNSAREAILGSIRTHLAASPVVDDHPVNPANPVILSKSDPVNR
                     FKESVESVAGHCIITTDVTDSLNQIITDLNAQRIAYSDNPPNAHDIFHFDVGISTAQA
                     AIAETGTLVLDSACERHRLVSLVPPVHIAIINASAIVETLSDALTFLQKKEISPAITF
                     ITGPSRTADIELTLAIGVHGPQELYVIVDGSK"
     gene            complement(24633..>25043)
                     /locus_tag="VFH96_08445"
     CDS             complement(24633..>25043)
                     /locus_tag="VFH96_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HET6974040.1"
                     /translation="ACPVYQQVGGHAYGSVYPGPIGAVITPQLMGIEKAAQLPYASSL
                     CGACREVCPVKIDIPQLLLHLRGEISPRKGSVAERLAFKIWARVMTSPSLYRLSSVAG
                     RILQLAMPISRAWTNDRDLRPIESKTFHDLWSNE"
BASE COUNT         5626 a         7290 c         6698 g         5429 t
ORIGIN      
        1 tgatgaaaag gcttttcttc gggtccatcg gatcgaattc ttcatagcga tcaagcgctt
       61 ccaggattcg ctcatccgaa ggcaacacga ctagttcacc atggatcatg gtgcgcgacg
      121 aagggtcgag caccgcaccc ggaaattcac caaaatcgta gagccttcca cgaacgtggg
      181 ctgatccaag ccgtcgaaaa cgcttaacaa tggaagcaat ctctttcgga gcttcacccg
      241 gaaggagagt gccgtataag aagagatgtt ggctcatatc ctcgatgcga gtaacttagc
      301 caggccttca tagccattct cgggatttga tatataagtc ttcacccgat cgtgtctatt
      361 caaacaatca agataataac cgaaaagcac gtctatttct gagtctttga gctgcttcga
      421 agttctcaga aaggcaagga actcgaagaa gtttaagtaa tcagtgaaag ctggctcttc
      481 ccgaattaca atctcgttaa ctgaatccga atcgttcaag caatcaagct tgtctcttat
      541 ttgcttgaga cctactccct catagaactt ctcgtaaaga ctcgacgccc atctggcacg
      601 ttcaaaagtg gaattgcgtt ggtaaacaaa ggcggcgaat acagccgcga ctaccgtacc
      661 gactgccgag attgtgcccg caattaaata gacaatctca acccgttctt tcacagtcat
      721 ttaattctcc tgcagattct gttttagatg ggttatgagg gcgagcaatt ctgcgtgacc
      781 tgggccacct aatgcaagtg cctttgtgcc tttttgtggc taatcaaaat aatcgaattc
      841 tcgtcagaat gccggagcca ccggttagtt tgactcacct ggtccatggt ttcccctcgg
      901 tatatcagca aatacccact ggcacacgag tggcttacag ccgggtggcg aatagccgca
      961 cgctttgaca acccacgagt ggtaagcccc cagttaccca cttagctcta tccgaaccga
     1021 acattactgt gagccggtta tgtttctcga tagcaactct tttatctccc gcgccagccg
     1081 aaagctttgg cccctcccta ccttcgtgat tccatccgcg cttggattct gcttcatagt
     1141 ctccgcgctc ttcggatcca atctcactga attcgtcgtt gttcttatcg tggcccaggt
     1201 aatctcgagt gttgtcgtct ggaaaagctg gcgcggcact caggaacaac tcgccggcgt
     1261 gagacacgag ctatcgagcc tgcaaggcat cgtcgacgtt tcgcgcgacg caatcatcgg
     1321 cgtgaccacc gaaggcgtga tcatgagctg gaaccgcggc gcacgcggca tctatggcta
     1381 cacttccaaa gaagctctcg gagcaccaat ctcgatgctc ttcgatcatc gtcgtggcca
     1441 ggaagcttcg ttattgtttg aaaaagtcac gcgcggcgag aacgtcacgc aacacgagat
     1501 ggtccacctc aagaagggcc gcactccgat cgacgtctcg ctcacgatct gtccgatcat
     1561 ggaaggcaaa accatgactg gcgcctcagt ggtggcccgt gacatcaccg agcgcaagcg
     1621 cgccgccggc tctcttgccc aacaagccgc ggcgatgaaa gcgtcgatgg acggcatggc
     1681 tatcatcgac cataccggcg tctgcattta tctaaacgac gcttacgcga aaacttttgg
     1741 tcacgcggat cccacgcgac tgatcggcgc gagctgggaa atgttttact tcgaggaaga
     1801 gctcgctcgc atgaaagagc agatcatgcc cgctgtctgg cgcgacggct catggcgcgg
     1861 tgaggcaatg ggtctgaaac tcaacggcgg cacttttcca ctcgaaatct cgatctcgag
     1921 tgtcgatggt ggcggtctgg tccaggtcgt gcgcgacatc accgagcgaa agaacctcga
     1981 agaaaccctg cgtaacagct cgctgaaaga cgatctaacc ggtctgttca atcgacgtgg
     2041 attactcaaa caggccgcgc cttatttcga cttcgcgcgc cgccagaaag aaaagctgct
     2101 gctgctcttc atcgacctcg acggcatgaa gaagatcaac gacgagtttg gacataacga
     2161 aggcgacaac gcactcatcc aaaccgccgc gattctcaat cacagtttcc gcagctccga
     2221 catcatcgcg cgtctcggcg gcgacgagtt tacagtcctc gtcaccgatc tgaacgcaag
     2281 taaagaagaa gcaatcgcga gactcaatga aaacctgaga gcctacaacg cgagcgagac
     2341 tcgtaatcac aagctgtcct tcagtatcgg tgtagcgacg ctcgagccgg agcgcatggc
     2401 ttgtttcgaa gagttactcg aacaagctga tcaggcgatg tacgagcaga agcgcagcaa
     2461 gcgccgccgc acatccgagc gctctcagaa cgaagcacag tacgcgcccg attcgatccc
     2521 gtttgcgctc ccgacaaatt ccgttggccg tcagggcact tttgacaacg ccgcgatcgg
     2581 aatggccgtt gtctcagtcg acggcagttg gctacaggtg aacgaatcgc tttgcaaact
     2641 gctcggctac tcagaacaag agttgcgcgc gaccagtttt caacgactca ctcataccga
     2701 ggatgtgcga cacgtccagt cgcatattca gcgcgtgctc gaaggctaca ttcacagtca
     2761 cgagcaggag aagcgttaca ttcacgagca gggccacacg gtttgggtgc agtggcacgt
     2821 ctcgcttttg aaagattcgg aaagcggcgc gaagcgtttg ttcttccagg tgcaagacat
     2881 cagcgatcgc aagaaggccg aggagaaact gacgcaagac acgctgactg gtctgccgaa
     2941 tcgcgcacgc ttctacgatc tgctcaagct gcgcgtctcg aataaaactc ggcagtgcgc
     3001 ggtgttgctg ctcgacgtcg atcgcttcaa gcttgtgaac gacagtcttg gcaatgcaag
     3061 cgcggatcaa ctgttgatac agatcgcaca gcgcgttaag acgtgcatgc gacagggaga
     3121 cgtactcgcg cgcgtcggtg gcgacgagtt tgccgtgttg ctcgacgatg tttcgggtga
     3181 ggaagaggcg tcgtcagtgg caacgcgcat ccagcaggca ctttcgatct cgttcaacct
     3241 gctgggccag gaagtttaca cgacgatgag catcggcatc gcactcgctt ccgattacga
     3301 gcaggtgtca gacatgctgc gcgatgcaga aacggcgatg caccaggcga aggcgcgcgg
     3361 caaagcgcgt tatgaagtct tcggacacga catgcacggc gagttgatga gccgtttgaa
     3421 gatggagact gatctgcgtc acgcgtgtga gcgcgatgag ctcttcgttg actatcaacc
     3481 aatcgtgtcg ctcggaagtc gcacgctgat tggattcgag gcgctcgtgc gctggcgtca
     3541 tccggagttt gggatcatac cgccgcagga tttcattccg gtcgcggaag agacgggcca
     3601 gatcctgacg atcggacaga ccgtgctcga gtcagcgtgt cgacaggcgc gcgagtggca
     3661 acagacttat ccggtggcgc cgccgttgtt cgtcagcgtc aacttgtcag tgaagcagtt
     3721 taatcagccg ggtttggttg agaacatcgc cgggttgctg gagcggttcc aactgccgcc
     3781 gcgttgtttg aagctcgaga ttacagagtc agtgtttact gacaacatcg aggcggcggt
     3841 cgggttgttg acgcagttgc gagagctcgg tgtgcagttg agtattgatg atttcggaac
     3901 gggttattcg agtctcagtt atttgcaacg cttcccgatc gacacgctga agatcgatcg
     3961 ctcatttgta acgcagatga tggagaacga ggagaacctc gcgatcgtgc gcaccatcgt
     4021 tgcgctcgcg cagaacctcg ggatggatgt agttgcggaa ggtgtcgaga ctgaagatca
     4081 gttgaaactg ttgcgaaagc tcgagtgtga gaatgggcag ggttatttgt tctcgacgcc
     4141 actcggcggg tggcagttga atgagtttat cgcggactac gtcgatgcac cgacggcgtt
     4201 agtagcgtag agccggcaaa ggctgcacag aaccgagcgc ggtagcgccg ggtcaacttc
     4261 accacatggt gctcttaatt gaggttgacc cggcgctacc gcgctcggtt ctgtgcgcta
     4321 cgagacttga tagaggtcgc ggcctgcatc tgcaacgatc aacgtgtccg gatcggagcg
     4381 catcgcttca taatcaagcg tgcgataatc gaggtagccg agattgacgc ggcggcaggt
     4441 ttcttcatcc aggccggttg ccattgtaat ttgataacgc ggaacgacct tgccatgctg
     4501 gtccaacctt ccagcgtaag cgacgtgcgc gaggtgggcc gcgatgcaca ggttctcacg
     4561 aagctcctgc gacacaccaa gcatgtctcg cacgctctcg agcggcgcgt atccgtactt
     4621 ctcgattaat tcaccgtgcg tctcggaaag ttttgtcagg tgcggtgcgt agattatgag
     4681 ctcgccgcct tcctcgatga tcgcgccgag tttgtaactc gccttccctc caacccaaag
     4741 ctcgtcgtaa tgctgatcga gcagcgcgat cacgcgtttg tatttacgtc cggtgtaacg
     4801 aatatggacc tgacgactga cttcagcggc acgtcgaaaa gcttcgcgaa tatcaccggt
     4861 gaagagcgcg tacgtcacca gctcaccgtt atttctggaa actacagtgt tcaaagagat
     4921 gatgcgcgcg gaaatcagat cggcggctgc ttcgatcaaa cggcgcgtag gtgtttcgac
     4981 ttgtccgatg atgttttcga tgccggcgag cgcgccgagc cagtgagtag tgtgcgtcaa
     5041 ctccggccct gcgacgcccg gaaagaaata cttcgcacca cccgcaaagc ccgcaacttc
     5101 gtgtgggacc gtcgcgccga agacgagcac cgtgtcgtag attcccggtg cgagtagctt
     5161 gtttatactg accggcaccg cattatcgat caagccacct gttaactcac cgactgcctc
     5221 agcactcaat tcaccgagcg tgatcaattc atcaggatca tcccagcgat gatcgaacaa
     5281 agatccgcgg aaatccgcgt tcccgatctt cgagaatttc tgcgccgcag acatcggcgg
     5341 atgcgtgccc tgcgcaacga gtgcgtcgaa agacgcaacc ccgcgtttcg ttaagaactc
     5401 attggcgatc gggaaaagct cgtgagtgtt atcgtcccgc gtcttgtcgg aaatgatagc
     5461 gagaacgcgc tcaccgggct ggatcacctc caactcttgc cataaaattt tcctaagatc
     5521 agcggtagtt aacataaata tttagccaca gattacacgg ataaaaaatc cgatttcatc
     5581 cgtgtaatcc gtggcccggt tttacaacga gactgtttgc tcagcccaga attgaaactc
     5641 gcggtgcttg tgcgtttcgg ctttggcttc gagaccgctc gcaacctcga tcacgtgatc
     5701 gaatacgcgc ccacccacct cgtcgatcgt ctctgttcca tcgataacgc cacccgccga
     5761 cagatcgaga tcgttcttca tgcgctcaaa gatcggcgtg tttgacgcga gcttgatcac
     5821 cggcgcgatc gcgtttccga tcgtcgtgcc gcggccggtt gtgaacacaa ctacagtcgc
     5881 accggccgcg acgagtccgg gtgtcgactc ctgatcgtat cccggccctt gcatcagatt
     5941 cagcccgcgc gtcctccagc tctccgcgta ttccaacacg tcctcgacac gcgtcgtccc
     6001 cgcttttgcg atcgctccga gcgacttgat cgtgatgttc agcagtccac ccgtgatgtt
     6061 accgggactc ggattctgtg cgagcaccgc accgaatttc gacgcatact ctttgtacca
     6121 gtccaccatc cggtaaactt ctcgacccgt ctcaacatcc ttcgcacgac acgccagcaa
     6181 atgctcggcg ccacaaaact ccggcacttc agttatcaac acggtgccac ccgatcgcac
     6241 gagcagatcg gctgcgcgcc cgagcgacgg gttcgcagaa attcccgaga acccatcaga
     6301 tccaccacac ttcacaccca gcacgatctc actcacggaa cactcttcgc gcgccgcacg
     6361 attcacctcg gacagcatct gctccacgtg tttgagcccc tgctgaatcg cggcttgcgt
     6421 gccgccaact tcctgaatgc cgatcttcgc caccggctta tcaaacgact tctcgcgctt
     6481 gagcagatac ttctcgactt gcgacaggtt cgtcttctcg cacccgaggt cgatcaagat
     6541 cacgccgccg acattcggat gatccgcgta gtttgaaaga gtgcgcaaca tgacatcgat
     6601 cgtcgatcca tcctggcaac cacaaccttt gttatgcgga atcgcaacaa cgccatcaac
     6661 gttcggatac ttgtcgcggt tgtaaagcgt gaactcagcg atcatcgaga tctgctgcgc
     6721 ttcgtgactc gcgcacatgc tggtcggcac gatgagcaca tagttgcgcg tgcccacgcg
     6781 accatcgccg cggcgaaagc ccatgaaggt gcgacgctcg gcttcaggaa tgtagtcagg
     6841 tggaggcgtg tgcagcgaat cgggtagatc gcgaatgagc ggcacgtcgt tcgacatgtt
     6901 ggcgtgcgtg atctgctcgc cgctctggat gcctaacgat gttccgatcg gttgaccaaa
     6961 ctgagtaaca aactctccgg cgggaatgtc gcgcgtggcg aagcgatgtc cgggcggaac
     7021 ggcatcgcgc agtttcacga gtgcgctatt cggcagcagc acttcgagat cgacgaaggt
     7081 ctcctttttg acgacggcaa cgttatcatt cggaccaacg acgatcgcat actcgcttat
     7141 tggtgtttct aacttagaca tgctccccaa tgttatccac agattacaca gattacgcag
     7201 atctgtgtaa tctgcgtaat ctgtggatca agacgcccgc gcctcgcgca caatctcaat
     7261 aaactgtttc gcccgctcag taatcaacgc gctttgccct tcgcgaatcg ccttgatgtc
     7321 aacgaggtca gctccgacac cgagcgccgc agcaccagcg cgaataaagt ccgccgcagt
     7381 cttgagcgag acgccgccag tcggcacgag ctcgatctgc gggaggggcg ccttcaggga
     7441 tttcagatag ctcggcccac caaccgcgcc tgcgggaaac accttcacaa aatccgcacc
     7501 ggcattccac gcctggacca cctccgtcgg cgtcaacgca cccggcatca ccgcaatatc
     7561 aagctcgcgg caacgcgcaa tcgtttcgag attgagcgcg ggactcacaa taaactgcgc
     7621 accactcgag acacacgccg atgctgtttc cgcatcgagc acagttcccg cacccacgag
     7681 ggcatcgttt ccgtaacgct tcgcaacgtc ttcgatcact ttcaccgcgc ccggcacggt
     7741 catcgtaatc tcgagcaccg agatcccacc ctcgcgaatc gcatcgatcg cgcgcatcgc
     7801 ttcgtcagcc gtcgtcgcgc gcaccacggg aatcacaccc gtgtctctga tatgtttaat
     7861 gacatccgtc ttcatccgtg ttaatccgcg gcccgggttt tacctggcaa cgcgcgccgt
     7921 cccgcctttc atcactcgtt cgacttcaga caatgtcgcc atcgtcgtat cacccggcgt
     7981 cgtcatcgcc agcgcaccat gcgccgcgcc gcactcgacc gcccattgtg gtccacgctc
     8041 ggtaagaaaa ccgtaaatca atcctgacgc gaatgaatca ccaccgccga cgcgatcgag
     8101 tatctccaga ttctcacgca ccggcgcctg gtaaagttcg ccaccggtgt aacaaacagc
     8161 gccccagtcg ttgatggtcg cggtcttcgc attccgcagc gtcgtcgcca cgactttgaa
     8221 gtttggaaac tcagccacag cgcgctggat catgcgatga aatccggcga cgtcgagttt
     8281 ggaatggtgc tcgtcctgtc cttcgacttc aaaaccgagc gcggcagtga agtcttcttc
     8341 attaccgatc atcacatcaa ccaggggcgc cagctcgcga ttgacagcaa tcgcccgtgc
     8401 ctggccacca atcgacttcc aaagcgattc gcgatagttg agatcgtacg agatgatcgt
     8461 gccgtgcttg cgggcgactt ccattgcttc acgcgccaca gccggcgtgg tttcagagag
     8521 tgcacaaaat attccaccag tgtgaaacca gcgagcgcct tctacaccga agacctgttc
     8581 ccagtcgatg tcgcccggcc gcagttgcga aatggcagtg tgaccgcgat ccgagcaacc
     8641 ggcagccgcg cgcacgccaa agccacgctc ggtaaagttg agaccgttgc gaaccgcgcg
     8701 tccgacgccg tcaaacttca cccacttcaa gtgcgactgg tccacgccac cctggtagat
     8761 cagatcctgg accagccggc ccacggcgtt gtcagcgagt gccgtaacaa ctgccgcatc
     8821 cattccgaag catcgtttaa gcccgcgcgc gacgttgtac tcaccgccgc cttcccaaac
     8881 ctgaaactga cgcgtcgtcg ccacacgccc ctcgcccgga tcgagccgca gcatcacctc
     8941 cccgagactc acaatgtccc agcgacaacc actcttcgat ttagccacga aagcactcat
     9001 aagaatttag ccacaaaaag gcacaaaaag cacaaaagag aagaactgaa tagaagttct
     9061 tttttgtgct ttttgtgcct ttttgtggct catcttttct taaaggttag ctgggtcgag
     9121 cctgggtgac aataaatgaa agagaagtaa tgccacaagg taggccgagg cggcaatgat
     9181 aaagatgggc acgtagctgc cggtgcgctc gagaatctcg ccgaccaggc gcgcgatcat
     9241 catgccgccg actgcgcccg ccatcccacc gatgccaact accgatccga ctgcgcgtcg
     9301 cggaaacatg tctgacgtcg tcgtgaagag attgcacgac cagccctggt gagccgccgc
     9361 ggcgagcccg atcaacaaga ctgccaccca gagattggac gtctgcgccg cgaacacgat
     9421 cggcacgact gcaatcgcgc aaatcaacat cgctaacttt cgcccggcgt tgatggtcca
     9481 accacgcttg atcagcgacg acgagagcca gccgccgccg atgctgccga tatctgccat
     9541 caggtaaaca acgacgagcg gtggtccaaa gtccttcaag ctcagcccgt gacgtttgtg
     9601 cagaaagtca ggcagccaga agagatatac ccaccagatc ggatctgtca tgaacttcgc
     9661 gaccgagaaa gcccacgtct gccggtgcgg cagcagccgt ccccacggaa tgttgactac
     9721 tggatccggc ggatcgctct gaatgtattc gagctcggct tttgatagtg acgtgtgctc
     9781 ttccggacgt cgataaagca gcagccacgc gacgagccag ataaaaccga tcgccccggt
     9841 ggcgatgaaa gcggcgcgcc agccccacgc aatcgtgatc atcggcacga cgagcggcgt
     9901 cacgagggcg ccgatgtttg tgcccgagtt aaaaattcca gtcgcgagtg cacgctcttt
     9961 cttgggaaac cactcagcga ctgttttgat cgccgcggga aagtttcccg cctcgcccag
    10021 accgaggaaa aaacgcgccg caccgaagcc gagcacagtg tgagccaacg catgggccat
    10081 cgccgccacg ctccagaaga tgatcgcgag tgagaaaccc ttgcgtgtgc cgatgcgatc
    10141 gatcagacga ccgacgccga gcaagccgat cgcatatgcc ccctgaaatg agaagacgac
    10201 ccaactgtag tcaacttcgc tccagccgat ttcgtcgtgg atcgttgttt tgagtatgcc
    10261 gatgacctgg cgatcgatgt agttgatggt cgcggcgaag aacaggagcg cgcagatgac
    10321 ccagcgaaag ccgaacgcgg atgaacgctg attttcaacg cggattgaga cctgttcttc
    10381 gatcatcttc ccatccagcc tccatcgacg acgagtacgt ggccgtttac gtaatcactc
    10441 gcgcgcgagg aaagaaagac tgcggcgccg gcgacgtctt cgggttcgcc ccagcgaccg
    10501 gccgggatgc gctcgagaat ctgccggttg cgtgtttcgt cagcttgcag cgctgctgtg
    10561 ttagtggtgc gcatgtatcc gggcgcgatc gcgtttacgt ttatccggtg tttggcccat
    10621 tcgtttgcaa gcgccttcgt gagctgggcg acgccgcctt tcgacgcggc ataagcaggg
    10681 acggtgatgc cgccctgaaa tgaaagtagc gaagcgatgt tgacgatctt gccgccgcga
    10741 ccgctgtcga tcatctgttt gcctgcgagt tttgaaagac gaaatacact cgagagattc
    10801 acttcgatga cggcggtcca atcgtcgtcg gaatagtcga ccgcgggcgc gcgtcgaatc
    10861 gtgcctgcgt tattgatgag gatgtcgatg cgtccgaagc gatcgagtgt ttgtgcgatc
    10921 agatcctgtg ctgtttcggc tttgctgaga tcgcccgtga ttggatgtgc aacgccgcct
    10981 gcgctctcga tctgatcgca ggttgattcg ggcgagcgcg tgttgccgtg acaagctact
    11041 tgtgcgccag cttcggcaag cgcgatcgca atcgcagcgc caagaccggt cgacgcaccg
    11101 gtaaccagag cagcgcgctc gtgcagctta aattgatcga gaatcatttc aggaaatctt
    11161 cacgcagcgg ggagaagata tcgatcagca ccacctcttc atccatcatc gtggcgccgt
    11221 gccagcaatt tgacgggaag tgcagcacgt cgccggcgga cgcgatctgt tccttgcctt
    11281 cgatgaagaa ccgcacgcgg ccgcgctcga cgatcgtcat ctgctcgtgt ggatgatcgt
    11341 gctcgggagt caccagaaac ggcttgaaac gaaaacgaca gatcatcatc cgattgccga
    11401 cgaacatctg tcgctcgatt ccctcggccg ggtgctcgac gggaatagtg gtccagttca
    11461 aatgccgcgg gaacccgtta ccgtttccgt tgccgtggtt ctcagaagtg gtggtagaca
    11521 ttttccgtgt cctctacttc ttgtcgctaa cagcttccaa tcccgatccg ccggcggcgt
    11581 attcgggttg cacgttcacg actccgtaaa gccgatcctc gccttccctg atcgcggcca
    11641 tcatccacag gaagttgatc tgcccgcccg gcgccgcgac gttcgggtga taaccctgcg
    11701 gcatcaacac acaatcgcct tcgcgcacga cctggaccaa ctctggctta ttcggatccg
    11761 tgtagacaaa ctgcacgcca aacgagggcg acggcatgtc gataaaaaga taagcctctt
    11821 cgagtagttt tgagtgttcg tgaggaggcc atgacgtcca attgccgggc gtcgagaaag
    11881 ttacaccggc catgatgcgg cccgcttcga cattcgcgcc gatcagcacg ttcaagtcgc
    11941 gctctgcggg tggcttgccg gcgatcagat gcagcttcgg gttctggcgc acttctttgt
    12001 acgagacaaa cttcagcgga tatcgcttag cgacgggcgc tgagacttca gcgagatcgc
    12061 cgccgtcagt tgtgcttact tcgatctcgg aatcacgcgg gacgtagacc gcgtcgtagc
    12121 gatcgagatc gaatgtttcg ccttccgcgc gtacagttac tttaccgttg agacagatca
    12181 gtccgacttc gtgatcgttg ttgttgaact gcaacggtgg atcaccagcg gcgagcgtga
    12241 tgcggccata atgcagaaaa cgcgcggcgg tctcaccggg agcgaccgca gtgcggcgcc
    12301 ctttgccttc gtgggtcttc ggcacgaaac acgtctccgc ggtgattggg ttttgattta
    12361 cttcctggtc catgaaaact cctcacattt ttagacagga ttcacaagat ttttcaggat
    12421 taacatgtaa atcctgttcg atcttgtaaa tcttgtttca caacttacct gggcgtcagc
    12481 gcgatcttat atttcaacgg cgctggaaca tcgtgcgctt cgaacacgag atgcaacggt
    12541 atcgcgccat gcactccacg tccaagcgcg ctattgccac tcgctacgat cgacgtcttc
    12601 aagggaaaat ttgcgtcgat cattcgcacg tccaacgatg ccttcgctgt atagaaccga
    12661 tcgacacgca cgcctttaat catttcagtt tcgacctgat cgtacgtgct cggcacagcg
    12721 atccgccagc gacgaatagt aactccgcct tttgaactca atgtttcctc gcgcgtcaag
    12781 gtgccattct caatcgccca cacgacttcg ctcaccagcc caacatcctg caccttcccc
    12841 gacggcgtac caacaacagc ccatttattc cagcgcacgc gcaacgaccg accatcactc
    12901 gccggctcga tcacatctgc tccatcggtc gcaaccacgg tccgaccatc tgcaagctca
    12961 acatacggcg tcaacaccgg atagatctgc tccaccggcg cagcaaaacc cggcagtcca
    13021 tgcggcgccg gtaaataatc tgacatgccc ggctttggac cagtcgtgat cggcaacgca
    13081 aatcgcaacg cgccctgcct cacaacccaa acacctgccg atcgttccgg cgactttcta
    13141 aaaaattcaa accgcgccac gtcacgcaac gacggcacaa ccggaaacgt cgcaatccct
    13201 tccttctcca acgcattcac gatcggcgtg tgcgagtttg ccagcttccc aaaaaagccc
    13261 gtcgtctgct gccactcacg atcgcgcgag atgtaagcat agttgccgcg gccaaaggca
    13321 aaaatcgaga gcaggtgcgt atcgctgcga taatcgtgca gcaagtaatt ccatgcctgg
    13381 taatacagcg cggcgatgtc agcgagcggc gcgggtctga actccggatg cgaagccaga
    13441 aacccggcga tctcgggcgt gtcgagataa ctgaccacgc cctggctgcg cccccagttg
    13501 taaccgtatc catcctccga aacaacgtcc caccacaaac gcatctggac catcaaggac
    13561 ggccgcactt tgtccaacag gtctttgcgc ccggcgctct ccgctgcttc ccagatgtag
    13621 cgcgcatact cgtttgaata acgatcgaaa cgtccgttcg gcggtgcatc gtcggcgtag
    13681 agattccctt ccgtgaactg caccgcagcg cgatcgagca gcttgtcgag aaaggcgcga
    13741 tcgttcgaga ggccgagctc gtaaccgata ctcgcgatgc gcgccgcgac gccgagatag
    13801 ttctcagcca gattgatgac gcgattcgtt ttcggatcgt aaaagcgttc gggattcagc
    13861 aacgagcgcc aggccttctg ctcctccggc gacaaactct gccaaagtgc attcgttttc
    13921 aaatcgcgcc cgaagtgtcg caaagcgagc gcggcgtaga gctgcgaaaa cgctttgctg
    13981 cccgcgcgcg attcgatcag accaatctgg cccacccact tcgcgatgtc acgtcccgcc
    14041 agctcacgat tgcccgtcgt ctctgcgtac gcggcgagcg agcgcataaa cgtgccccac
    14101 gaatactcac ctgtcgtcgg cacaccgaga atctgatccg gcggcggatc gagcgacttg
    14161 atcgcgctca ggtgcgcggc gagttccttg ccgagaaacg tgtttacgtc gtcgcgcaag
    14221 ctggccgttg ttacggtgct cccctgagcg gtaataactg cggaaacaca gatgacgcag
    14281 atcaagactg atttaaatcc tctaatcatc cgtgttatct gtggttagaa attaagcttc
    14341 aaaccaaact gaatattacg agcgggccca gccgtcgtga tgcgaccaaa ccctcccgtc
    14401 gtcagaacag tagcgggtga cctaaagtta gctcggttga aggcattaaa catgtccgcc
    14461 tgaaagcgaa ggttgaaacg ctcggaaagc gtgaactgct tgcgcatcga gaaatcccag
    14521 gtttgcagat tcgggccctg aacgatgccg acgccgctgt taccgcgacg agtagcagga
    14581 gcgggcgcaa acgccgcatc attaatccat tggttatttg gagcaaagtt cgctgcccgg
    14641 actggcccgc ctacataatc cgcgcgcctc gttcctattg ccgtattagc tgtgacagtt
    14701 aaatatggtc cgctttgata gcgcgtgata ccgctgattt cccagccgga caacactgcc
    14761 ccaccaaccc ctttcatctt gctaaaccat ggaagcagat agtcatacgt ggtcacgaag
    14821 atgtgccggc gatcaaaggt tgccaagcca taatttgagt ggcggttaaa tggatcctca
    14881 ccgacatcaa cgccgtcgcc attaccactc gtatcggtca acgccttgga ccaggtatag
    14941 ctggcggtga gctcaagatt gcccttgcgc ttggccgcgt aaagctgcat gccgttgtag
    15001 ttagagttgg cgtcagacag ccggtagagt atctgagtaa atcctttaaa aggacgcaac
    15061 gcgtcagtat tcacatttgg accaccgttc gcaggcagat ttgccgtcaa ggctttgaat
    15121 gaaacctgat tgaggtctgg ctgtcgaatc aagtgtcgac ccaaattgcc gacgtagttc
    15181 gcctcgatga agaaaccacg aggtagctct ctctggacac caagactaaa attcatcgag
    15241 taagcgacgt ccagattggg atcgatggag ttaatcgagc caatcaaggc tgcattcgct
    15301 ggagttccgc cggcaggatt cgcaagattg ccattgctca gactgggcgt actaacaaat
    15361 ggcggcgcgt taacctgcga gaagatgaga ttaccttcga ctttgtcgta gtacatacca
    15421 aagccgccgc gaatggaagt cttgtcgtca tggaacgggg cgtaggcgaa tccaaatcgc
    15481 ggcgctatgc gatctgccgg ctgataaaaa cctcgcggcg cgccggcagg aacgagggcc
    15541 agcgccgcat cgctggtggc gactcggccc cgctcgtcaa tgggaatgcc atcaccggct
    15601 ctgactaatc cgttaaagcg attacctcct tttgtcagat cgattgtgcc gttggaaagg
    15661 actgtcaccg ccttcgatgg atcgtagtga ctcgggtcga agttcgccat gttgtttgcc
    15721 tgcgtgtacg ttggaccaaa atgatagaaa cgagctccaa tttcaaaact cagatcgcgc
    15781 gtaatcttcc aactgtcgct aacaaaagct tcgacctggt tgaatctgaa gaaaccgatc
    15841 ggatcattgt cggcttcact ataggtgcgg aaatttccca gcaaggcatc tgcaaaagcg
    15901 ttgcccgtag taacggcgcc actggcagca ccggaattga aagcgacgtt gccagtatag
    15961 atagaccgcc cgttctgatc tttgcgatta cgaactacca ggacgccggt cttgagcgaa
    16021 tgcttaccgc gaatgatggt gatgttgtca ctgacggcga tatcagcagt cggcgacaat
    16081 aaagacccgt ttggtccatt aaagtttgcc atcgcgccgt ttccggcgaa tgtaacgttc
    16141 ggaatgccgt cgtcgaaacg tccacccgaa gaaagctgtg gataggtaaa accaaacgta
    16201 gagcgtttcc agaattcccc taccggcggg attcgctgac cattccagga cgcattggct
    16261 ttagcctcat tgattaaagt tggtgttatt agccaggtat aggagagttg ataactactt
    16321 cccggacgaa gtcgattggt tggcactgtc ggcaattgcg aatttatgaa tgtgccgaag
    16381 ggatcgatca ggtcgtactt atcgtggaga tagcgaccat agacactatg tttggaattg
    16441 attctgtaat ctatgcggat gttgtcttca cggtaatcga aaggattagg ctgctgaaaa
    16501 gtcgcgttgc tcgccgtcgg cgtatccgag taggcaactg cgattttctc catagcggta
    16561 tagaccgctg cgattgctgc gccattagga gtgacaccaa ctggagctcc gttgagtctc
    16621 aggtttgcca gattacgccc tggaattggt tgcccggcgg cgactgttgc tccgttcgcc
    16681 ggattgattg ccgtgtagcc gaccggaacg ttcagcgcgc ccgtacgtaa actaaagtcg
    16741 cctaaccgtt cggctctggt cggcaggttg cgggaaacgg gagcggcgtc ctgacggatg
    16801 tacttgtact cctgaccgac gaaaaagaag agcttatctt taacgatcgg gccattgaaa
    16861 ttccagccaa aattgttgaa gcgcaacttt ccttttttcg gtgagaaata gttgcgggaa
    16921 tcgagtttgt cgttacgtcc gaattcgtaa gcactaccgt gatagtcgtt tccaccactc
    16981 cgtgtaacaa cattgacgga agcaccagcg tttcgaccgt actcagcaga gaagtttgag
    17041 gtttgaatct tcacttcctg aataaagtcg atgccgacgt tattaacctg actattgttg
    17101 ctgcccgaat ccatgttgga gccgccatca actgacaggc tgttgtagtt aggtcgattg
    17161 ccgttgatag cagcctgact gatactcaag ctcgttgtaa gagcgagctg gtcttcgtca
    17221 agaatcgccg cgccgggaat aagcgtgacg agctgcatgt agttgcggcc gtttagagcg
    17281 agattttgaa cctggcgctt atcgacgacg cgagcaactt cacctgacgt tgtgttgacg
    17341 gtttcgcctg ccgcggtgga gacttccacg gtctctgaaa cagcgccggc ctcaagagta
    17401 aggttgacag ttagccgcgt gtcagcagct aaaacgttat cggtctgaac tgccttcttg
    17461 aatccggttt gttctacgga gatggtgtag ctccctaccg gaagattggt aaccgtgtag
    17521 aagccttcac cgtctgtaac cgcggaccgt tccaggttag tcgcgttgtt tcggatcttc
    17581 actgcagcgt taggtaaaac tgcacccgaa ctatctgtga tggtgccgga aatgcgcccg
    17641 aagattgttt gaccgctcgc gcttacagcc gcgaaggcga taaacaagat aacagcacaa
    17701 atgggaaagc atcttgatgc ggagcttcga ttattttgcg tccattgcat gtctagcacc
    17761 tccaaagatt gtcggaatct gattggacta cttagccgct acgcgaccat cggagccgcg
    17821 aaggctggtg cggccacgag ggcaactgcg ccgcgcaggc gcgggtgatc ttccgcgggc
    17881 acgacgtgga tctccgtcgc gccgccaaac tcagttaacg tacgctcgcg tagcgcacta
    17941 cgcgcgatcg gctcgatcaa actccacgca gtcgtgattt cgccgccgat gtaaacgcgc
    18001 gcgggatcga gtgcgttgac gatcgaagcg agaccgagac cgagatagcg tgctgtcgag
    18061 ttgagtgctg caagtgcttt cgcctcgccc gcacgcgcac gtgcgatcaa gtcttcaaca
    18121 ctgaagcctg cttcctcgga atcacgcagt cgcggatcag acaagtcgcg accgaagtaa
    18181 cgggacagag tcgccaggtt ggaaacataa gcttcccagc aaccggtcgc accacatgag
    18241 cactttggac catcgatgct caacggcacg tgaccaaact cacccgcagt gttgtgacga
    18301 ccgcgcagca cctcgccatt aacgatgatg ccgacaccga cgccgtccga aacactcacg
    18361 aagaccgagt ttcccgaagc aacaacgtcg ctgcgcgtgg cccatagttg agcgagagcg
    18421 cacgccttac cggaattctc gatttgaacg ggaatgccca acgccgccgc aagcggatcg
    18481 cgcaggttga cgtcatgcca ggcaagtgtc ggcgcatgga ccacacgacc cttcgtcggc
    18541 tcaaccatac cggggaccga cacaccgacg cctgcacacc cattgtaatc ggcgaggatc
    18601 tgcttgatgc gacgcgcgag ctcctggatc aaagtatcga catcacgatt agtttgaaaa
    18661 ctgctgaccg cgccgagctg ccggccgacg agatcggtga cgaggatgta agtgcgcgac
    18721 gcgcgaatgt cgacgccgac aacgcagcgc tcgcgggagt cgatataaag aaatgtggga
    18781 cggcgaccac ggccgactga ctcaccgaga gcgccctcga agatcagatt ttcactcaac
    18841 agttcgctta ccagcagact ggcaacgccg cgccgcacat tcatcagccg ggcgaggtca
    18901 gcgcgcgaga cgggctggtg tgtgcgaatg agatttagag cgatctgccg gttgatctcg
    18961 cggctagtac cccgccgggc aaccgtgaaa tcgtgtgggt taatcttgcg catgagcgat
    19021 attgttcttt attaagacta attagacgaa ctatttgcag cctaaagaac aggcctgctt
    19081 tattcggatt tatttgggta actgagacga acgaatttgt ctaaacgcgg aacatatact
    19141 cttctgaatg cgggctgtca acaattttct ttgttttatg ccgcggattt tcgcagatga
    19201 acgcggatcg atccgcgttt tatccgcgtg aatccgcgtt tccactggac tgtcgaataa
    19261 cgagctctgg agggatggtg atcgtccgat tcttcttctt gccatcgatc aattcgagca
    19321 ggagtcgcgc cgccgtctgt cccatctcgg cctttgacca ggaaacagtg gtcagcggta
    19381 cacgtaacat atcaccataa tgaatactcc ccgcgccgac aaacgcaata tcgtccggca
    19441 ctttcaaacc ggcttcattc gctgccgcca tcccgccgat cgctgccgga tcgtttgccg
    19501 caaagatcgc cggcggtagc tcaccggacg cgatcaactt ctgcatcgcc gcgtaaccat
    19561 cagtttcagt aaacccgcaa tcgtggacca acgctttgag cctcgctttc gacatcgctt
    19621 cacgataacc ctgcaatcgc ttcaacgccg tggagacatc cggtccgcgc aggtgaccga
    19681 tcttacgatg accgagcttg atcagatgct cagtcgctag tcttcccacc tgcacgtcgt
    19741 cggtcgtcac cgccgaacag cgcagtccct caagcacacg atcgatcaaa acgatctttg
    19801 caccgtctga aattagtcgt ttgtaaaatt tcacctcgct tgctgacaac gccgacgcga
    19861 cgatcagccc atcggtgcga gacaagagcg cctcgatctc atccatctcc ttgcgtggct
    19921 cttcgtccgt gctacagatc agattctgat acccgaccgg tcgcgcgtgc gcttccacac
    19981 cccgacagat ctcagcgaaa taagagtgca tgagatcggg aacaacgatg ccgatcagat
    20041 acgactgctt cgtcatcaac gcccgtgcaa actggttcgg gcgataattc atcttctgcg
    20101 ccgtcgcgag aatacgcgcg cgcgtctcac tactgatctc gggatgattg ttgatcgcgc
    20161 gcgacaccgt catcttcgag acgttgaggg cgcgcgcgat gtcttcgaga gttaccggca
    20221 tcgaatgtta cttgcgacgt ttcaaactcg ccgtgattgc ggcttcggcg agaggagcca
    20281 tcacgacata gccttgcgcg ttcggatgaa gaccgtcgtc actcagctcg tttttcagga
    20341 agcctttgtc gtcaaccatt gcggagaagt aatcgagata agtgtgatga tgcctggcgg
    20401 cgtactcttt caaccacttg ttaagctcaa gaatctgggc gggcggacgg cgcacggtct
    20461 gcacgatcgg tttaccatcg cgcatctcat aatcactgac cggcagcact gaggcaaaga
    20521 cgacgcggat gccgttcgcg gtggcgagct ccgccatcga gatcaaattg tcctcgatag
    20581 ccgcgagggt agtcggacca gtgttccccg ccagatcgtt tgtgccggcg agtatcacaa
    20641 cgactttcgg cttgagggca atcacgtcgg gacgaaaacg gataagcatc tgcggtgttg
    20701 tttgcccact aataccgcga ttgatatagg acttgccggg aaaaaatccg ccgtacttgg
    20761 gatcgtccca tgaatctgtg atcgagtcgc ccatgaaaac aacgcgtcgc gggccatcca
    20821 tcgacgccgg aacagtctta atattctcct cgccgtaacg ctcgagtgcc ggccaatctt
    20881 tcaatctcgc ctcggcacgg tccaaccgcg tcttcgtctc agcacaatcc ggactcggtg
    20941 tcgcaaccgg cgcctgcccg actgtaacta cggatgaaaa aactaaaacc gcccaaatca
    21001 ttatgaatgc cttcgccata cgtctccttc gacaggattt acaggatttt caggattaac
    21061 aggtaaaccc tttgtgcttt ttgtgccttt ttgtggctaa gatcttgatc tccagcgatc
    21121 gatcgccacc gccaccacaa tgatcgcccc gataatgatc tcctgaatgt agttgggcca
    21181 acccacctgc tggcagccgt tgcgcagaaa cgccatgatc agcgcaccaa tcatcgaccc
    21241 gagtatgctt ccctcgccac cactcaaact gccaccgcca atcaccaccg cagcgatgat
    21301 gtccaactcc gatcccgcag cgactgtcgg atcgccttgt cgcaagcgcg acatctgcat
    21361 cacgcctgca agtccaaaca acaaccctgc gatcccataa atccaaatct tgagcctgtc
    21421 agtggcgatg ccacacgcgc gcgccgcagc ttcgtttgaa ccaagcgcaa acacacgtcg
    21481 tccaaacacg gtgttacgca gaacgatcgc cgcacaaaca gcgagaataa ttgttatcca
    21541 cacgcccggc gcgacgagca accacgatgg tttcggaaac atcaccagca gctcattcag
    21601 ccacgtgccc ggcatgttga ccgtctgttg attcgccagc cacttcgcaa ccccacgcgc
    21661 cacgccgagc attccgagcg tcgcgatgaa cgggacgacc ctcaagcgtg tgatcacaac
    21721 tccattgatc acaccgacga acccgccaac taaaattccg ctcaacaaag cgaccgctgg
    21781 tggtaagcct gcgttgatcg cgagcgcggt gactacgccg gtcaaagcga tcgtcgatcc
    21841 gacggcgaga tcgataccac cgctgatgat gatcagcgtc atgccgatgg cgccaatcgc
    21901 aacgatgact gtttgcgaga gaacgatgcg aagattgttt ggtgagagaa gcctgcccgg
    21961 tgtatcgact aataacgaga agacgccgat cacgagcagc aatccgagca ccggcccgag
    22021 acggttcaga ttctgtttga gatcaaccat tcaagtaaac gcggattata cggataaaaa
    22081 tccggatttt atccgcgcaa tccgcgtttc attttaggta ttggtctagt ggcggatgga
    22141 gtaatgcgtt cacttccggt gattgcaggt tttctttcgt cacgagcatc acgcctgtgt
    22201 cgatcttctt ctcgaccggt ttgccgagca ggctgtcaac catcgtgcgc acgcccaggt
    22261 aacccatgtt gaaaggattc tgcacgacga tgccgtggag ctggttagcg ttgatggcgt
    22321 caatgaaagt ctggctcgaa tcaaaaccga caaacaaaac ctttcccgcc ttgccgatgt
    22381 cctgaagcgc gagcaacatc cccgcggtcg acgattcatt cggcgtgaag atcccctgca
    22441 ccacgtcacc aaaacgattc agcagatttt ctgacgcgcg cttcgccgtg tcgcgtgtcg
    22501 cgcctgcgta ctgatccgtc gagacgagct cgatctccgg aaacttcgct ttcatttgtg
    22561 aaatgaaacc ttcttcacgc tccgtcgtgc tcgcggatcc ttccgcgtaa cgcaacacga
    22621 gcaccttgcc tttgccgttc agcagttggc ccatcctatc tgccgcgagc gtgccgcctt
    22681 tgcgattatc agtggcgacg aagctcacaa actcatccga ctccaaaccc gaatcgatga
    22741 tcacagtcgg cacacccgcc cgctccgcat cggcgactgg ccgaacgagc gcgcgattgt
    22801 cgagcggtgc gagcacgatg ccgctcacgc cctgcgccgc aaaaccctcc accacctgta
    22861 tctgctgctc gcgatcgtct tcgcgcaacg gtcccttcca gatcacttcg acttcggtgc
    22921 cttgcgcggt gagctcgctg gcggctttgt tgctgccggc gtgaatgctc ttccagaact
    22981 cgtgcgtcgt gcctttcgga atgacggcga tcgtgagttt cttcttcgcg ttgttggtgg
    23041 atggaccacc gtgcggcgta cacgcgatcg cgaaggtcaa agcgagcaat agcagaagta
    23101 atttgcgcat gttgttttcc tgttaagagt tttcctcact gactacggga ttccccaagc
    23161 cgcccatgcc ttcgatcagt acttccatcc gatcgcccgg cttcagaaat accggcgggt
    23221 tgcgtgcgaa gccgacgccc ggaggcgtgc ccgttgcgat cacatctccc ggctcgagcg
    23281 tgatcgattc cgttagaaac tcgatcaact ttgggatacc gaagattagt tgatcggtgt
    23341 ttgagtcctg catcgtctgg ccattgagca ccagcttgat cgagagcttg tgcggatcgg
    23401 ggatcgcgtc ggtggtaacg atcgtctggc ccatcggcgc aaacgtgtca cacgattttc
    23461 cgcgctgcca ctgcccgtcg ccaaactgaa aatcgcgcgc gctcacgtcg ttgaaagccg
    23521 tgtagccaca cacgtaatcg tacgcgcgat ctgctttaac gtgcttcgcg tgccggccaa
    23581 tcacgaccgc cagctccgct tcgtagtcca cttgttgact cgtcgacggc aggaccaccg
    23641 gctcgccggg cgcgatcacc gcggtcgaga acttcgaaaa cacaaccggc ttctcgggaa
    23701 tcggcatctt gctctcagcg gcgtgatcgc gatagttcaa tccgatgcaa atgatttttc
    23761 ccggacgcgg cacgggcgca aaccagtaag catcacgact cggaaccagc cagcccttgt
    23821 cgcgcgcttc agcgagcgcc tgcttgtctt taatcagtat atcgtgtgtc gcgcgtgctt
    23881 tctgaaacca tgggccatcc atgtcaaacc acgcgagcgg atgcgacggg cgctccgctt
    23941 cgggaaacag cttctcgcaa tcgaggcgat agcccgtgtc ttgaccgtcc acctccagaa
    24001 tcatgccgag gcgcggcaca ctccatttgg tttcctgtgt ggcgaaagaa atgatttgca
    24061 tctactttga tccgtcaacg atgacgtaca actcctgtgg gccatgcacg ccgatggcca
    24121 gagtcaactc gatgtcggcc gtgcgcgatg ggccagtgat gaatgtgatt gccgggctga
    24181 tttctttctt ctgtaaaaac gtcaaagcgt cgctgagcgt ctcgacgatt gctgaagcgt
    24241 tgatgatggc gatgtgtact ggtggaacaa gtgacacgag tcgatgacgt tcgcaggcgc
    24301 tgtcgagaac gagcgtgccg gtttcggcga tggctgcctg tgcggttgaa atgccgacgt
    24361 cgaaatgaaa tatatcgtgt gcattcggag gattgtccga gtaggcgata cgttgcgcat
    24421 tcagatccgt gatgatctgg tttaaagaat ccgtcacatc cgtggttatg atgcagtggc
    24481 cggccacgga ttcgacggat tctttgaacc gatttaccgg atcggattta gacaggatga
    24541 caggatttgc aggattaaca ggatgatcat caacgaccgg actcgccgcc aggtgcgtgc
    24601 ggatggagcc gaggattgct tcgcgtgcgg agttattcat tggaccacag atcgtgaaaa
    24661 gtcttcgact cgattggacg caggtcgcgg tcgttcgtcc acgcgcgtga gattggcata
    24721 gcaagctgta gtatccggcc ggcgacggag ctcagcctgt ataacgatgg gctggtcatg
    24781 acgcgagccc atatcttgaa cgcgagacgc tcggcgacgc tgcctttgcg cgggctgatc
    24841 tcgccgcgca gatgtaacaa taactgcggg atgtcgatct tcaccggaca aacttcccgg
    24901 cacgcaccac acaagctcga cgcatacgga agctgcgcag ctttttcgat acccatcaac
    24961 tgcggcgtga tcaccgcgcc aatcggacca ggataaaccg acccgtaagc atgaccacca
    25021 acttgctgat acaccggaca cgc
//
LOCUS       DASSFQ010000231        11215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1598093, whole genome shotgun sequence.
ACCESSION   DASSFQ010000231 DASSFQ010000000
VERSION     DASSFQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598093"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..552
                     /locus_tag="VFH96_08450"
     CDS             67..552
                     /locus_tag="VFH96_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6974041.1"
                     /translation="MTKVFSLIALVILVSGVAAAQTQTAPTDENAAITKTALNYIEGW
                     YEGNAERMESALHPELAKRMIYTDPKSGRSQFNHMGAMTLVQGTRRGGGSQTPKEKQL
                     KEVTILDRFQNAAVVKIVASDWIDYLQVAKFNGEWKIINVLWELKPKPPVQSTSSTTK
                     P"
     gene            661..11136
                     /locus_tag="VFH96_08455"
     CDS             661..11136
                     /locus_tag="VFH96_08455"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET6974042.1"
                     /translation="MFDSLVHLLEVRAGERPDRLAYTFLRDGEVEEANLSYGELACAA
                     RAVGAWLQERKLQNQNVLLLYPPGLEYIVAFWGCLYAGAVAVPAYPPSRNRHLGRIEA
                     IVADAKPQLALTTATIRARIESSLACGVTEEIDPELAKNWRVPHIDHSTLAFLQYTSG
                     STATPKGVMVTHGNLLHNEELIRRAFHQNEHSIIAGWLPLYHDMGLIGNVLQPLYLGA
                     RCILMSPISFLQQPLRWLRAISDYRATTSGGPNFAYDLCTQKISVAERDSLDLTSWSV
                     AFNGAEPVRAETLHRFAEFFEPCGFRREAFYPCYGLAEATLFVSGASGPSIKTSVVGC
                     GALEHDPEVVIVNPETFVRCAPGEEGEIWISGPSVAAGYWNQPEETASVFHARLADGD
                     RGPYLRSGDLGFVGSDELFITGRIKDLIIIRGRNLYPHEIERTVEKSHPSLKPGCGAA
                     FSIADEGEERLVIVQELARRASSSFDDVLGNIRQAVVEEHEVQPYCVVLVSAGSVPKT
                     SSGKIQRGRCRELFLSSRLEVIASWQANKQTVLTDEGVVEVETVLRSHLAAKLGVDPE
                     SIDVHAPLTRYGIDSLSAIEMAHAIESNFNVEWSMASLLEGPSLFEVATQLRAKMDHA
                     TKPVVTPIRTNGTHALTHGQQALWFLHRMNPANAVYNVAAAARIQGALDTNALEKAFL
                     ALAQRHASLRSTFDVVAGQPMQRIHEHLPLHFESIDASSWSEIDEWCAEEALRPFDFN
                     AGPLWRVSLLKRRSDEHVLLIVAHHIIVDFWSLNVMWQELGELYTAQREERQPRLPVL
                     ESQYAGYVRRRAELLDGPEGERLWTYWQRQLGPDLPVLDLPSDRPRPPVQSHRGTAYT
                     FKIDPALTQAIKTVAHDAGATLFATLLAAFETLLYRYSGQESFAVGSPASGRDSAALS
                     HLVGYFVNPLVMRADFSGNPSFAELLSRVRRTALDALRHQAFPFALLVQRLQPERDLS
                     RTPLFQTMFVFQPSQVLNSFAVGEAGSRVKLGELNLESVALTQRTTLFDLRLTMTEAD
                     DTLLASFEYSTDLFDGERIRRMAGHFETILASIVKNPQQRVSELSFLTPAEQRQLVVD
                     WNTGTIDYPREQCMHELFEEQVARTPEATALVHDDERLSYAELNERTNRLAHYLRTFG
                     VGPETLVGILLERSPLMIVGILGVLKAGAAYVPLDINYPRERLQYTLQDARAQVLLTQ
                     AELHSALPETEAEVVIIDERGQATLPDLFNPRSSLGRTSQEEGLAPAAQDLAYVIYTS
                     GSTGRPKGVAIEHRSAVAFIHWAKEVFSIEHLAGLLASTSICFDLSIFEIFVPLCVGG
                     KIIIAAQALELPTLSAAEEVTLINTVPSAMTELVRLHAVPDSVRVVNLAGEPLPNALV
                     QSIYQQAGIEQVLNLYGPSEDTTYSTWTLVEKGSTEAPTIGRPIANTQAYLLDAHLQL
                     VPVGVVGELCLGGHGLARGYLNVPELTAERFIPDPFSTEPGARLYRTGDLARWLPDGQ
                     LEFLGRRDHQLKLRGFRIELGEIEAVLKESRTVEDAVVIAREDTVNDVRLVAYVVGCD
                     NTTELRDHLKQKLPHYMIPSAFVVLDKLPLSPNGKIDRRALPAPEALTPDVENDFVAP
                     RDQREEMIAGIWAAVLNVEQVGIHQNFFDLGGHSLLATQVIARLNEAFHVDLPLRSLF
                     ESPTVAALAERVIHSSGAHYAQPIEHVARDGPLPLSFAQQRLWFLDQLEPGSAAYHVP
                     ILIRLTGPLNASTLEQSIREVVNRHESLRTTFKNVDERPVQVIAPPGSWRLNAIDLSE
                     TDEPEREALRRAKREALLPFDLSTGPLLRATLWHVGADIHILLLAMHHIVADGWSIGI
                     FVSELSAIYNAAPLAELPCQYADFAQWQRRQLSGEALATHLDYWKQRLRGPIPPIELP
                     SRRVRSTTNDVSAATESMPFPEELTTSLQQLSRHHGVTTFMTLLAAFQTLLHRYTGLD
                     DIAVGTPVANRTRTETESLIGFFANTLVLRGDVSGDPSFDELLTRVRTLTLEAHAHQD
                     VPFELLVEELQPERDLDRTPLFQIMLVMQNTPPPVIDLPGVRVEQLELETTAPKFDLV
                     LFLKESDGSLSGSWEYNPARFDAETIRGLASHFETLLESAIADPSQRISQLNMLRDDE
                     RQLVVSKWNDTAVEYPRAAGLHKLFETQAARTPKATALVYDDERLTYEELNERANQLA
                     HHLRGSGVGPETRVGICLERSLEMVIGLLGILKAGAAYVPLEPSYPQERLSLMIEEAG
                     IRVVLTTQRFREILSVDAICLDADWNQIARQSKANPATNTVPGNLAYVIYTSGSTGKP
                     KGSMITHEGICNRLLWMQDAYRLTAADRVLQKTPYTFDVSLWEFFWPLITGARLVMAR
                     PGGQGDSRYLVETIRQEKITTMHFVPSMLAVFLEEREAGECRSLKRVICSGEALSYEL
                     KEKFVKLLPAELHNLYGPTEASVDVTYWDCRSELERAIVPIGRPIANTQIYVLDKNLQ
                     VVPVGVPGELYIGGAGLARGYLNKPDLTAERFLPDPFGGARLYRTGDLARYLADGNVK
                     YLGRTDHQVKLRGFRIELGEIEAALNEHSAVQQAVVVARDKRLVAYVVPDPQRAAPIN
                     RLLGLEAAGQLENLTQFELPNEMTIVCRNRNETEFMYEEIFADKTYLNFGIRFPNDAC
                     IFDVGANIGLFTLFASRLAPQSVIYSFEPIPPVFEILRRNAEIHAPDTKLFACGLSNV
                     SEQASFTYYPNVSLISGRFADVVADGETVKSFVMGQQGGNGNGLLSAEQLDELIAERL
                     TSERFTCQLKTLSEVIRAEGVEKIDLLKIDVEKAELEVLAGIDDADWPKIQQIVMEVH
                     DLDDRVSQVTELLSGHGFAVNVQQDAMLEQTNLYNLYARRNSQTVSHEIDEPAAVVWS
                     SRELLVKELRERLKNRLPEYMVPSAFVLLPELPTTSSGKVDRRALPDPAVESIGAHVE
                     PRTPVEEQLAQMWEQLLNRTRVSVHQNFFELGGHSLLATQLMSRVRDVFQIELPLRSI
                     FEAKTIAELAKRIERGTGTMSPIPRASRDTALPLSFAQQRLWFMDQVVPGNPAYNIQA
                     AVRLSGPLSPTAFDSTLKEIVQRHEILRTTFHVREGQPTQIVGAIVELRLEIRDLSTL
                     QSSSDAIQQLASEEARRPFDLTRGPLLRAMLLRLAETEHVLLLSVHHIVADGWSMGVL
                     VREVATLYRAFVEGRPTPLAELPIQYADFAVWQRQWLQGEVLHEQLDYWRQQLAGLEP
                     LELPSDYPRPPVQSFRGARRFFSLSAQISNELRALSEREGVTLYMTLLAAWQTLLHRY
                     TGQNEIVVGTDIANRNRSEIESLIGFFTNQLVLRSNFNGNPTFRELLRRVRQITLDAY
                     AHQDLPFEKLVETVQPERIFDRNPLFQVMFVFQNAQQLELPGLVVRALEIDEGTTAFD
                     LTLTMEETDEGIKGSIRYSTDLFKATTIERMVQHYQNILTSLVTSIDTPVHLLEMLDP
                     EEAQVKKRSKLKKLSTVTPRAVTVP"
BASE COUNT         2426 a         3163 c         3223 g         2403 t
ORIGIN      
        1 gggtagtaga atacgcgcaa tctgatccac agattacgca gattttactc ggaaaaggag
       61 tctcgcatga cgaaagtatt ctcactgatt gcgcttgtta ttctggtttc aggcgtcgcg
      121 gcagcgcaga cgcaaacggc accaaccgac gagaacgccg cgatcaccaa aaccgccctc
      181 aattacattg aaggctggta cgaaggcaat gcggaacgta tggagagtgc gctgcatccc
      241 gagctcgcca agcgaatgat ctacacggat ccaaaatccg gacgtagcca gttcaatcac
      301 atgggcgcta tgacgctggt ccagggaaca agacgcggtg gcggcagtca aacaccgaaa
      361 gagaagcagt tgaaagaggt gaccatcctc gatcgtttcc agaatgcggc tgtcgtgaag
      421 attgttgcgt ctgactggat tgattacctg caggtcgcga agttcaatgg cgaatggaag
      481 atcatcaacg tgctctggga gttgaaacca aaaccgccgg tgcaatcaac ctctagtacc
      541 actaagccgt aaatcagttc agccacaaaa ggcacaaaag gcacaaaata ataatgtgcc
      601 atttgtctgg ttgttttatc ttgtgccttt tgtgcctttt gtggcccagt cttagtaacg
      661 atgtttgaca gtttggtcca tctcctggaa gtgagagccg gggaacgtcc cgatcggctc
      721 gcctacacct tcctgcgcga cggggaagtt gaggaagcta atctctctta cggggaattg
      781 gcgtgcgctg ctcgtgcggt gggcgcgtgg ctgcaggagc gcaagctgca aaatcaaaac
      841 gtgctcctgc tttatccgcc cggactcgaa tacatcgtcg cgttctgggg ttgcctgtat
      901 gcaggcgcgg tcgctgtgcc tgcgtatcca ccgtcgcgca atcgccatct cggacggatc
      961 gaagcgattg tggctgacgc aaagcctcaa ctcgctttga ccaccgcaac catccgcgcg
     1021 cgtattgaat cgtcactcgc ctgcggcgtt actgaagaga tcgatccgga actggcaaag
     1081 aactggcgcg tgcctcacat cgatcactcg acgctcgcct tcctgcaata cacctcaggt
     1141 tctaccgcca caccgaaggg tgtgatggtc acgcatggca atctgctgca caacgaagaa
     1201 ctgattcgtc gcgccttcca tcagaacgag cattcaatta tcgccggctg gcttccgctt
     1261 tatcatgaca tggggctgat cgggaatgtg ctgcaaccgc tttatctcgg tgcccgctgc
     1321 attctcatgt caccgatttc gtttctgcag cagccgttgc gatggctgcg cgcgatttct
     1381 gattaccgcg ccactaccag cggcggacca aacttcgctt acgacctctg tacccaaaag
     1441 atctcagttg cagaacgtga ctcgctcgac cttaccagtt ggagcgtcgc tttcaacggc
     1501 gcggagccag tgcgcgccga aacgttgcat cgcttcgccg agtttttcga accgtgcggc
     1561 ttcaggcgcg aagcatttta tccctgctac ggtttggcag aagcaacgct cttcgtttcc
     1621 ggtgcgagcg ggccttcgat caaaacttcc gtcgtcggct gtggcgctct ggaacacgat
     1681 cccgaggtcg tcatcgttaa tcccgaaacc ttcgtgcgat gcgcgcctgg tgaagagggc
     1741 gagatttgga tcagtgggcc gagcgtagcc gcgggctact ggaaccaacc cgaagagacg
     1801 gccagcgttt ttcatgctcg tcttgctgac ggcgaccgtg gtccatatct gcgcagtggc
     1861 gatctcggat tcgtcggctc agacgagtta tttatcacgg gacgcatcaa ggatctcatc
     1921 atcattcgtg gccgtaacct ctatccacac gagatcgaga ggacagttga gaagagtcat
     1981 ccctcactga aacccggctg cggtgcggcg ttttccattg cagacgaagg cgaagaacga
     2041 ttggtgatcg tgcaggagct tgcgcgtcgc gcttcgtctt ctttcgacga tgtgctcggc
     2101 aacattcggc aagccgtagt cgaagagcac gaagtccagc cttactgcgt ggtgctggtc
     2161 agtgccggta gtgttcccaa aacttcgagc ggcaagattc agcgtggaag gtgtcgcgag
     2221 ctgtttcttt cttcgcgact ggaagtgatc gctagctggc aggcaaacaa gcaaactgta
     2281 ttgactgatg aaggtgttgt cgaagtagag acggttctac gctcgcatct ggctgcgaag
     2341 ctcggtgtcg atcccgagag catcgacgta cacgcgccgc tcacgcgcta cggtatcgat
     2401 tctttgtcgg ctatcgaaat ggcgcacgcg atcgagtcga acttcaacgt ggaatggtcg
     2461 atggcgagtc tgcttgaagg tccgagtctc tttgaggtcg ccactcagct ccgcgccaag
     2521 atggatcacg ccactaagcc agtcgttact ccaatccgga cgaatggaac gcacgcgctt
     2581 acacacgggc agcaggcgct gtggtttctg caccggatga acccggcaaa cgcggtttat
     2641 aacgtcgcgg ctgcggcacg cattcagggt gcgcttgata cgaacgcgtt ggaaaaagca
     2701 ttcctcgccc tcgcccagcg acacgcctca ttgcgaagca cgttcgatgt ggtcgccgga
     2761 cagcctatgc aacgaatcca cgaacatctg ccgcttcatt tcgaaagtat cgatgcttct
     2821 tcgtggagcg agatcgatga atggtgtgcg gaagaagcat tacgcccgtt tgacttcaat
     2881 gccggaccgc tatggcgagt gagtctgctg aagcgacgca gcgatgagca tgtgctcttg
     2941 atcgtcgcac atcacatcat cgtcgatttc tggtcgctca acgtgatgtg gcaggaattg
     3001 ggcgagctct acactgctca gcgtgaggaa cgacagccac ggcttccggt tttagagtcg
     3061 caatatgccg gctacgtgcg gcggcgggcc gagttgcttg atgggccaga aggtgagcgg
     3121 ctgtggacct actggcagcg acaactcggt ccggatcttc ccgtgctcga tcttccgtcg
     3181 gacaggccgc ggccgccggt gcaatctcat cggggaacgg cgtacacgtt caaaattgat
     3241 ccggcactca cgcaagcgat caaaaccgtc gcgcacgacg ctggcgccac acttttcgca
     3301 accttgctgg ccgcgttcga gacgctgctc tatcgctata gcggccagga aagcttcgcg
     3361 gttggatcgc cagcgtcagg acgagattcc gcggcgttat ctcaccttgt cggttacttc
     3421 gttaacccgt tggtgatgcg cgccgacttt tcgggcaatc cgagtttcgc agagttgttg
     3481 tcgcgcgttc gccgaaccgc cctcgatgcg ctacgccatc aggcgttccc attcgcattg
     3541 ctggtccaac gattacaacc cgagcgcgat ctcagtcgca cgccgttgtt tcaaacaatg
     3601 ttcgtgtttc agccttcaca ggtgctgaat tcatttgcgg tgggcgaagc gggatcgcga
     3661 gttaagttag gcgaactgaa cctggaatcg gtggcgttga cacagcgaac aacgctcttc
     3721 gatctgcggc tgacgatgac tgaggcagac gatacattgc tggcctcgtt cgaatacagc
     3781 accgatctgt tcgacggaga gcgcatcaga cgaatggccg gtcacttcga aaccatactc
     3841 gcgagcatcg tcaaaaatcc gcaacagcgc gtgtctgagc tctcgttctt aacgccggcc
     3901 gagcaacgtc aactggttgt tgactggaac accggcacga ttgactaccc gcgcgagcaa
     3961 tgcatgcacg agttgttcga agagcaggtg gcgcgcacgc ccgaagccac ggcgcttgtt
     4021 cacgacgacg aacggctgag ctatgccgag ttgaacgaac ggacaaaccg cttggcccac
     4081 tacttacgca ccttcggcgt gggaccggag acactcgtcg gcatcctgtt ggaacgttca
     4141 ccgctgatga tcgtcggaat acttggcgtg cttaaggctg gtgccgccta cgtgccactc
     4201 gacatcaatt atccgcgcga gcggctgcag tacacgctcc aggatgcacg cgctcaggtg
     4261 ttgctgacgc aggccgagtt gcattcagca ctgccggaaa cagaagctga ggtggttatt
     4321 atcgatgagc gggggcaagc cactcttcct gacttgttca atcctcggtc gtcgctgggg
     4381 agaacaagtc aggaagaggg gcttgccccc gctgctcaag atctcgctta tgtgatctac
     4441 acctcgggct cgaccggcag accgaaaggc gtagccatcg aacaccgcag tgcggtggcc
     4501 ttcatccatt gggccaaaga agtgtttagt atcgagcatc tcgcaggctt gctggcctcg
     4561 acctcgatct gttttgacct ctcgatcttc gagatctttg tgccgctctg tgtgggcgga
     4621 aaaatcatca ttgctgctca ggcgctggag ctgccaacac ttagtgcggc agaagaagtg
     4681 acgctgatta acacggtgcc ttctgccatg acggagctgg tacgactaca tgcggtgccg
     4741 gattcagtgc gcgtggtgaa tttggcgggc gagccgctac cgaacgcgct agtgcaaagt
     4801 atctatcaac aagcaggtat cgaacaggta ttaaacctgt atggtccatc agaagacacg
     4861 acctattcaa cctggacgct ggtcgagaag ggaagcacag aggcaccgac gatcgggcgg
     4921 ccaatagcaa acactcaggc ttacttgctc gacgcacatc tgcaactggt accggtggga
     4981 gttgtcggtg aactgtgcct gggcggccac ggattggcgc gcggctattt gaacgtaccg
     5041 gagctgactg ccgaacgctt cattcctgat ccattctcga ctgaacccgg cgcgcgcctg
     5101 tatcgcaccg gagatctggc gcgctggctg ccagacggtc agttggaatt cctcggccgc
     5161 cgcgaccacc agctaaagct gagaggtttc cgtatcgagt tgggcgagat cgaagcagtg
     5221 ctgaaagaaa gtcgcacggt ggaagacgca gtggtcatcg cgcgcgagga tacagttaat
     5281 gacgtgcgtc ttgtggccta tgtcgttggt tgtgacaaca ctacggaact acgcgaccac
     5341 ctcaaacaga aactgccgca ctacatgatt ccctcagcct tcgtcgtgct ggacaagctg
     5401 cctctatcgc caaacggcaa gatcgatcga cgcgcgctac ccgcaccgga agctttaaca
     5461 ccggacgtcg agaacgactt cgtcgcacca cgcgatcaac gcgaagaaat gatcgccggt
     5521 atttgggctg cggtccttaa cgtcgagcag gtcggaattc atcaaaactt cttcgatctc
     5581 ggcggtcact cgttacttgc tactcaagta atcgcgcgcc tcaacgaagc tttccacgtt
     5641 gatctgccac tgcgctcgct gttcgaatcc ccgaccgtag ctgctcttgc cgaacgcgtc
     5701 attcattcga gcggcgcgca ctacgctcaa ccaatcgagc acgttgccag agatggtcca
     5761 ttgccactct cgttcgcgca acagcgacta tggtttctgg accaactgga gcccggcagc
     5821 gctgcctatc acgtcccgat tctgatccgg ctcaccggcc cactcaacgc gagcacgtta
     5881 gagcaaagta tccgcgaggt cgttaaccgc cacgaatcgc tgcgcaccac attcaagaac
     5941 gtcgacgagc ggcccgtaca ggtgatcgca ccgccgggat cgtggcgcct taatgcgatc
     6001 gatctcagcg agacggacga accggaacgc gaagcgctgc gccgcgcgaa acgtgaggca
     6061 ctgcttccgt tcgatctctc caccggcccg ctgctgcgcg caacgttgtg gcacgtaggc
     6121 gctgacatcc acatcctgtt gctagcgatg catcacatcg ttgccgacgg ctggtccatc
     6181 ggcatcttcg tcagcgaatt gtctgcaatt tacaacgccg cgccactcgc cgaattgccc
     6241 tgccagtacg ccgacttcgc gcagtggcaa cgccggcagc tttccggcga agcgctcgca
     6301 acacatctcg actattggaa gcagcgtctc cgcggaccga tccccccgat cgaactgccc
     6361 agccggcgag tgcgctcaac cacaaacgat gtcagcgcgg cgactgagtc gatgccgttt
     6421 cccgaagaac tcaccactag cctgcagcag ttaagccgcc atcatggcgt cacgaccttc
     6481 atgactctgc tcgcggcttt ccaaactctg ctgcatcgct acaccggact cgacgacatt
     6541 gcggtcggca ctcccgtcgc gaatcgcacg cgaaccgaaa ctgagtcgct catcggattt
     6601 ttcgcgaaca ccctcgtgtt gcgcggcgac gtgtccggcg atccttcatt cgacgagctc
     6661 ctcacgcgcg tgcgcacctt gacgctcgaa gcacacgcgc accaggacgt gccgttcgag
     6721 cttctggtcg aggagttgca gcccgaaaga gatctcgatc gcacgccgct ttttcaaatc
     6781 atgctggtca tgcaaaacac gccgccgccc gtgattgatt tgccgggcgt tcgggtggag
     6841 cagttggagc tcgaaaccac cgcacctaag tttgacctgg ttcttttcct gaaagagagc
     6901 gatggaagtt tgagtggctc gtgggagtac aacccggctc gcttcgatgc cgaaaccatc
     6961 agaggcctcg cttctcattt cgaaacgttg ctggaaagcg ccatcgccga tccgagtcag
     7021 cgaatctcgc aactcaacat gctacgcgac gacgagcggc agcttgttgt tagcaaatgg
     7081 aatgacactg cggttgagta tccgcgcgca gcaggcctgc acaaactctt cgagacgcaa
     7141 gcggcgcgca caccgaaagc gacagcgctt gtgtacgacg acgaacgact cacctacgag
     7201 gagctgaacg agcgcgcgaa ccagctcgcc catcacttgc gcggcagcgg cgttggacca
     7261 gaaacgcgcg tgggaatttg tctcgagcga tcgctggaaa tggtgatcgg gctgctcgga
     7321 atactcaagg cgggcgcggc gtatgtgcct ttggaaccgt cgtatccgca agagcgactg
     7381 agcctgatga tcgaagaagc agggatacga gtggtcctga ctacgcaacg cttccgcgag
     7441 attctttcag tggatgcgat ctgtcttgat gccgattgga atcagatagc ccgtcagagc
     7501 aaagcgaacc cggcgaccaa taccgttccc ggcaatctgg cttacgtgat ttacacctcg
     7561 ggttccacag gcaaaccaaa gggctcgatg atcacgcacg agggcatctg caaccgcttg
     7621 ctgtggatgc aggacgcgta tcgattaact gccgcagacc gtgtcttgca gaagacgcct
     7681 tatacgtttg acgtttcgtt gtgggagttc ttctggccct tgattaccgg agcgcgactg
     7741 gtcatggcgc ggcctggcgg acagggcgac agtcgctatc tggtcgaaac gatcaggcag
     7801 gaaaagatca cgacgatgca tttcgtgccg tcgatgctgg cggtgttttt ggaggaaagg
     7861 gaagccggcg agtgtcgcag tctcaaacga gtgatttgca gcggtgaagc gttgtcgtat
     7921 gagttgaaag aaaagtttgt gaagctgctg ccggcggagc tgcacaactt gtatggacca
     7981 acggaagcat cggtggacgt gacgtattgg gattgccgca gcgagttgga gcgggcgatt
     8041 gtgccgatcg ggcgaccgat agcgaacacg caaatttacg tgttggataa gaacctgcaa
     8101 gtagtgccgg tcggtgtgcc tggtgagttg tacatcggcg gcgccgggtt ggcgcgcggt
     8161 tatctgaaca agccggatct gacggcggag cgcttcttac cggatccgtt tggcggagcg
     8221 aggctctatc gcacgggcga tctggcgcgc tatttggcgg atggaaatgt taagtacctt
     8281 ggccgcacgg accatcaagt gaagctgcgc ggatttcgca ttgagctcgg agaaatcgaa
     8341 gcggcgttga acgagcattc agccgtgcag caggcggtgg tcgtcgcgcg cgataaacgt
     8401 ttggtggctt acgtcgttcc ggatccacag cgcgccgcac cgatcaaccg gctgctcggc
     8461 ttggaagcgg cgggtcagct cgagaacctg actcagtttg agttgccaaa cgagatgacg
     8521 atcgtttgcc gcaatcgcaa cgagacggag tttatgtatg aggagatctt tgcagacaag
     8581 acctacctca actttggcat ccgtttcccc aacgatgcct gcatctttga tgtgggcgca
     8641 aacatcggcc tcttcacgct ctttgcgagc cgtttagcgc cgcagtcagt gatttattcg
     8701 tttgagccga ttccgccggt gttcgagatc ttgcgacgca atgcggagat tcatgcgccg
     8761 gacacgaagc tgtttgcgtg cgggttgtca aacgtgtctg agcaggcaag ctttacttac
     8821 tacccgaacg tgtcgctgat ttcagggcgt ttcgctgatg tggttgcgga tggcgagacg
     8881 gtgaagtcgt ttgtgatggg ccagcaggga ggcaacggta acggtttgct ttcagcggag
     8941 cagcttgacg agctgattgc cgagcgctta acgagcgaac gctttacgtg ccagttgaag
     9001 actttgtcag aagtgattcg cgctgagggc gtggagaaga tcgatctgct taagattgat
     9061 gtcgagaaag ctgagctcga ggtgttagcg ggcattgacg atgctgattg gcccaagatt
     9121 cagcagatcg tgatggaggt tcacgatctc gacgatcgcg tttcgcaagt gactgagttg
     9181 ctgagcgggc acgggttcgc ggtcaacgtt cagcaggacg cgatgctcga gcaaacgaat
     9241 ctgtataacc tctacgcgcg gcgaaattcg caaactgttt cgcacgagat cgacgaacca
     9301 gccgcagtcg tttggagcag ccgtgagctg cttgtgaaag agctgcgcga gcgactcaaa
     9361 aatcgcttgc cggagtacat ggtgccctcg gctttcgttt tactgccaga actgcctact
     9421 acgtcgagtg gaaaagtcga tcgacgtgca ctgcccgatc cggctgtgga gtcaatcggc
     9481 gcccacgtcg aaccacggac gccggtggag gaacagttgg cgcaaatgtg ggagcagctt
     9541 ttgaaccgca cgcgcgtgag tgtgcaccag aactttttcg agttgggtgg acactcactg
     9601 ctggcgacgc agctcatgtc acgcgtgcgg gatgtgttcc aaatcgagct tcctctgcgc
     9661 agcatcttcg aagcaaaaac aattgccgag ctcgctaaac gcatcgagcg gggcacaggt
     9721 acaatgtcgc ccattccgcg cgcctcacgc gacaccgctt taccactctc gttcgcgcaa
     9781 caacgactct ggtttatgga ccaagtggtt cccggtaacc cggcctacaa cattcaagcg
     9841 gcagtgcgac tctcaggtcc acttagcccc acggcgtttg attccactct taaagagata
     9901 gtgcaacgcc acgagatcct gcggacgaca tttcacgttc gtgaaggtca gcccacgcag
     9961 atcgtcggcg cgattgtcga actccgtttg gagatccgag acttgagcac gcttcagtcc
    10021 tcgtccgacg ccatacaaca gctcgcctcc gaggaagcgc gcagaccttt cgatctcacg
    10081 cgagggccgc tgctgcgagc gatgttgcta cgtctcgccg aaacagagca tgtgctattg
    10141 ctctccgtac accacatcgt cgctgacggg tggtccatgg gcgtcctcgt acgagaagtt
    10201 gcgacgcttt atcgtgcgtt tgttgaagga cgacccacac cgctggcgga acttccgatc
    10261 cagtacgccg acttcgctgt gtggcaaaga caatggctgc aaggtgaagt attgcacgag
    10321 cagctcgact actggcgtca gcaactcgcc ggtctcgaac cgttggaact gccgtcggat
    10381 tatccgcggc cgccagtgca gagttttcgc ggagcgcgac gcttcttttc attgtctgcg
    10441 cagatctcaa acgagctgcg tgcgctcagc gaacgcgagg gcgtcacgct ctacatgacg
    10501 cttttggcag cgtggcagac tctcttgcat cgctacactg gtcagaatga gatcgtcgtc
    10561 ggtacggaca tcgcgaaccg aaaccgcagc gagatcgaaa gtctcatcgg cttcttcact
    10621 aaccagctcg tgcttcgttc gaacttcaac ggcaatccga cattcaggga gctgctgcgg
    10681 cgcgtgcgcc agatcacgct cgacgcttat gcgcaccagg acttgccgtt cgaaaagctg
    10741 gtcgagactg tgcaaccgga acgcatcttc gatcgcaatc cgctgttcca ggtgatgttt
    10801 gtgtttcaaa atgcgcagca actggaactg ccgggcctcg tggtgcgcgc gctcgaaatt
    10861 gatgaaggga cgacggcctt tgacttgact ctgacaatgg aagagacaga cgagggaatc
    10921 aaaggctcga tacgttacag caccgacctc ttcaaggcca ccaccatcga gcgcatggtc
    10981 cagcactatc agaacatcct gaccagcctc gtcacctcga tcgacacgcc cgtgcatttg
    11041 ctcgagatgc ttgatcctga agaggcgcaa gtgaagaaga ggtcgaaact caaaaaactg
    11101 tcaactgtaa ccccacgcgc agtcaccgta ccctaactac gagtccttat ccacagatta
    11161 cgcagattgt tttatggcat ctgtgtaatc tgtggacaaa ataaccatga gtaca
//
LOCUS       DASSFQ010000232         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_551017, whole genome shotgun sequence.
ACCESSION   DASSFQ010000232 DASSFQ010000000
VERSION     DASSFQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551017"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFH96_08460"
     CDS             <1..715
                     /locus_tag="VFH96_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974043.1"
                     /translation="DEVTPFELKAFSYETEQSEVSGDLRVIYGREPLDLTIPMYQTLR
                     VKTTVAPPLYYIVPAQWTEVIDVLKAHGIQMKTLAEFHPVQIESYRFTNVSWPDGPFE
                     GRHMPRFDAEKTVETRVFPPGSAFVPVAQTKAKVILNLLEPEAPDSLAHWGFFNAIFE
                     EKEYAEHYVLEDLAREMMEKDPAVGQEFRQLLALDPEFAASPSARLRFFYKRSPYWDP
                     QMNLYPVGRIISVVDLPLA"
     gene            1288..3075
                     /gene="ftsH"
                     /locus_tag="VFH96_08465"
     CDS             1288..3075
                     /gene="ftsH"
                     /locus_tag="VFH96_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET6974044.1"
                     /translation="MPTPTPRVSVRTRRVALAGLAIITFSLVIAAAIATYQRHQPPVQ
                     KINYSQLYSLAQTGGATALAVEGETLTVTKADGSLQEATVTGETTQAEIVQLFRKQNV
                     PVEFRPLQPGMLSSIVSWALPILTLGLIGFAGWRIYSSMSGRGSFELENHNGKQNVGF
                     EDVAGVDEARAELTETIEFLRNPERFSRLGGRVPRGILLYGPPGTGKTLLARAAAKEA
                     GVAFLAASGSSFQEKFAGLGAARVRHLFAKGRKLSPCIIFIDEIDALGRKRGRGGDSA
                     TADQDQTLNQLLVEMDGFDQSSAVVVIASTNRPDILDKALTRPGRFDREIAVNLADVN
                     GREAILKVHSRNLKLEDGLDLTWIARGTPGFSGAELANLMNEAAIAATRDDASGIARK
                     HVEFARDRILMGAERQGFMMDDDERYATAVHEAGHVAVGLAVENGDPIHKVSILPRGR
                     ALGVTQSLPERDRFMKKREYLADQIALLLGGRAAEQVLLSTMTAGASNDIERAVEIAR
                     AMVSEFGMSRLGPIHLGQPDQQRSQSLLDKVEECTTELINEQLERACAIVTAERDSIG
                     RLVEMLMERDTLDAEEIANCFKRETVRAA"
     gene            complement(3158..>3877)
                     /locus_tag="VFH96_08470"
     CDS             complement(3158..>3877)
                     /locus_tag="VFH96_08470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET6974045.1"
                     /translation="APAPSNVQQAGASAYRDEPASQIRQTIAKRLVTSLGPVPHFFLT
                     TEIEMDRAAEMRKGINALDPDLKISINDIIIKVAAAALIQHPQVNASFQEKIVRYYEH
                     ADVGVAVAIEDGLITPVVRAADQKSLSQIAAEVRELAERARSKKLKPEEYTGATFSVS
                     NLGMFGIDEFTAVINPPEGAILAIGAMSAKPVVRDNEIVIRQMMRVTMSCDHRVIDGA
                     TGAKFLQTFKKILENPLYLVV"
BASE COUNT          853 a         1056 c         1084 g          884 t
ORIGIN      
        1 tgacgaagtc acgccgttcg aattgaaagc tttctcgtac gagaccgagc agagcgaggt
       61 gtcgggagat ctgcgcgtga tttacggtcg cgagccgctc gacttaacga tcccgatgta
      121 tcagacgttg agagtcaaga cgaccgttgc gccgccgctt tattacatcg tgcccgcgca
      181 atggactgaa gtgatcgatg tgttgaaagc gcacggaata caaatgaaga ctctcgctga
      241 gtttcatccg gtgcagatcg aaagttatcg tttcacaaac gtgagctggc ctgacggtcc
      301 attcgaaggc cggcacatgc cacgcttcga cgccgagaag acagtcgaga ctagagtgtt
      361 tcctccgggt tcagcattcg tgccggtggc ccagacgaaa gcgaaagtga tactgaatct
      421 actcgaaccc gaagcgcccg actccctggc ccactggggc ttcttcaatg cgatctttga
      481 agaaaaggag tacgcagaac actacgtgct cgaagatctc gctcgcgaaa tgatggaaaa
      541 agatccagcg gtcgggcagg agtttcgaca gctcctggca ctggatcctg aatttgccgc
      601 cagtccttca gcgcgacttc gcttcttcta caagcgttcg ccctactggg atccgcaaat
      661 gaatctgtat ccagtagggc gaatcattag tgtagttgat ctaccacttg cctgatttca
      721 ttgacgcccg acttaccaag cgtggtgtcg ggcgtcgggc agggcaaaca gtgtgcgagt
      781 ttgcctttaa ataactgttc ggcgaccacc gattatgttg ataagcagca ccacgagtgc
      841 taacacaagt agaatatgga tgaacccacc aaccgtgtag ctacttacta cgccgagtaa
      901 ccacaggacc aaaagaatta caaaaatcgt ccaaagcatt tcttcttccc tccttttgtc
      961 aaccacgccc caattttcgt tggtacaaaa gctatcgtcc tccccttgag caacgaccat
     1021 gccgtttctg tttcttggaa ttttcgcgaa attgacctat gcaggcgtac taaaacgagg
     1081 caatacgaag tagaccgata tgaatctggt tcagggtaat aggtctaata cgtcctattc
     1141 gccctatatt tcacgaagtt gttctggact tcacatatta gtcactggta aagtgcccat
     1201 tagctgtttt cgagcgggtc cttcccttcg aagttccgtc gctttgccaa tcccgattct
     1261 ttttaatctg tttggaggtc ttgttttatg cccaccccca cgccgagggt ctctgttcga
     1321 acacgcagag tcgctctcgc aggtctggca atcataactt tctccctggt tattgccgca
     1381 gctatcgcta cttatcaacg gcaccagccg cctgttcaaa agatcaacta ctcgcagctt
     1441 tatagtctcg cgcaaaccgg tggcgccacg gcgctcgccg ttgaaggtga gacgctgact
     1501 gtcacgaagg ctgacggctc gctgcaggaa gcaactgtga ccggcgagac cacgcaggcc
     1561 gagatcgtcc agctattccg aaagcagaac gtgccggttg aatttcggcc gctgcaaccg
     1621 ggaatgttgt cgagcatcgt gagctgggcg ttgccgatat tgacgctcgg actgattgga
     1681 tttgccggct ggcgcatcta ttcgagcatg agcggtcgcg gatcgttcga gcttgaaaat
     1741 cacaacggca aacagaatgt cggtttcgag gacgtggctg gtgttgacga agcacgcgcc
     1801 gagttaacgg agacgatcga gtttctgcgc aatccggagc gcttcagtcg ccttggtggt
     1861 cgagttccgc gtggaatcct gctttacggc ccgccgggaa ccggcaagac actcctcgcg
     1921 cgagccgcgg caaaggaagc gggcgttgca tttttggccg cgtctggatc gagcttccag
     1981 gagaagtttg cgggacttgg cgctgcgcgt gtcagacatc tgttcgcgaa gggaagaaag
     2041 ctttcaccct gcatcatttt tatcgatgag atcgacgcgc tcggacgcaa gcgcggccgt
     2101 ggcggcgact ctgccaccgc cgatcaggat cagacgctca atcagttgct cgtcgagatg
     2161 gatggtttcg atcagtcgag cgcggttgtc gttatcgctt caaccaaccg gcctgacatt
     2221 ctcgacaaag cgctcacgcg tcccggtcga ttcgatcgtg agatcgctgt gaatctcgct
     2281 gacgtgaatg gtcgtgaggc gattttgaaa gttcattcac gcaacctcaa actcgaggac
     2341 ggtctcgatc tgacgtggat tgcgcgtgga acgccgggct tctcaggcgc cgagctcgca
     2401 aacctgatga acgaagccgc gatcgccgcg acacgtgacg acgcatccgg gatcgctcgc
     2461 aaacacgtcg aatttgcgcg tgaccgcatt ctcatgggcg ccgagcgaca agggttcatg
     2521 atggacgacg acgagcgcta cgcgacggca gttcatgaag cgggtcacgt ggcggttggt
     2581 ctcgcggtgg agaatggcga tccgatacac aaggtttcga tcctgccgcg cggacgtgct
     2641 cttggtgtga cgcagtcact acccgagcgc gatcggttta tgaagaagcg cgagtatctc
     2701 gcagatcaga tcgcattgtt gcttggtggt cgtgccgcgg agcaggtttt gttgtccacg
     2761 atgacagcgg gtgcttcgaa cgacatcgag cgtgccgttg agattgcgcg ggcgatggtg
     2821 agtgagtttg gtatgagccg tctcgggccg attcacctcg gacagccgga ccagcagcgc
     2881 agccagagtt tgctcgataa ggttgaggag tgtacgaccg agttgatcaa cgagcagctc
     2941 gagcgtgcct gtgcgatcgt gacggcggag cgtgattcga ttggacgtct ggtcgagatg
     3001 ctcatggaac gagacacact cgacgcggag gagattgcga actgctttaa gcgcgaaacc
     3061 gtccgcgccg catagttcaa acataagtac acaaaaggca caaaaagcac aaaagagatg
     3121 aagaaattct tttgtgcttt ttgtgccttt tgtgtactca gacgacgagg tataacggat
     3181 tctcgaggat tttcttgaac gtctgtaaga acttggcccc ggtggcgccg tcgatcacgc
     3241 ggtgatcgca tgacatcgtg acgcgcatca tctgcctgat cacgatctcg ttatcacgca
     3301 cgacaggctt cgcactcatc gcgccgatag ccagtatagc gccctcaggc ggattaatca
     3361 ccgccgtgaa ctcatcaatc ccaaacatcc cgagattact gaccgagaaa gtcgcgcccg
     3421 tgtactcctc aggcttcaat ttcttcgatc gtgcacgttc cgccaactcg cgaacttccg
     3481 ccgcaatctg gctcagcgat ttctgatccg ccgcgcgaac caccggcgtg atcaatccat
     3541 cctcgatcgc caccgccacg ccgacgtcgg catgttcgta gtagcgcacg atcttctcct
     3601 ggaacgacgc attcacttgc ggatgttgaa tcaacgccgc tgctgcaacc ttgatgatga
     3661 tgtcgttgat actgatcttc agatcgggat cgagcgcgtt gatgcctttg cgcatttccg
     3721 ccgcgcgatc catctcgatc tcggtggtga gaaagaagtg cggcaccggc ccgagcgacg
     3781 tgaccagtcg cttcgcaatc gtttggcgaa tctgactggc gggctcgtct cgataagctg
     3841 aagcgcctgc ctgctggacg tttgaaggcg ccggcgc
//
LOCUS       DASSFQ010000233        14100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1142121, whole genome shotgun sequence.
ACCESSION   DASSFQ010000233 DASSFQ010000000
VERSION     DASSFQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142121"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..127)
                     /locus_tag="VFH96_08475"
     tRNA            complement(50..127)
                     /locus_tag="VFH96_08475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Arg,seq:gcg)
     gene            complement(134..219)
                     /locus_tag="VFH96_08480"
     tRNA            complement(134..219)
                     /locus_tag="VFH96_08480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(184..186),aa:Ser,seq:gct)
     gene            complement(226..312)
                     /locus_tag="VFH96_08485"
     tRNA            complement(226..312)
                     /locus_tag="VFH96_08485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(277..279),aa:Ser,seq:cga)
     gene            709..870
                     /locus_tag="VFH96_08490"
     CDS             709..870
                     /locus_tag="VFH96_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974046.1"
                     /translation="MLVVDVMTSNEQTSEYMAALGCHLIVCSLCFLIVNIEILLSCGT
                     HSRGASCCV"
     gene            complement(964..2013)
                     /locus_tag="VFH96_08495"
     CDS             complement(964..2013)
                     /locus_tag="VFH96_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974047.1"
                     /translation="MPEAKESHPLVELTLTRMREFLREKEAVFWVFVFPVLMTFALGI
                     AFRNTAPDKTLVAIEAGDAKASETASLLSHSNEITATVLSPDEAQRALRSGKVAIIVK
                     PGDKTFEYRFDPTRPESRAARLLVDDVLQRGKGRADVVSVSEARITEPGARYVDFLVP
                     GLIGMNMMGSGLWGLGFTVVIARSRKILKRFAATPMRRSHYLLSFMLSRLVFLVLEVA
                     AVIIFATLAFGFTVRGSWLSVALMTILGGFTFSGIGLLVAARPTTIEGVSGLMNFIML
                     PMWLLSGTFFSSERFPQILQPFIQALPLTALNNVLRALMNEGATLASNWIPIAILLVW
                     CVVSFVVALKIFKWQ"
     gene            complement(2006..2941)
                     /locus_tag="VFH96_08500"
     CDS             complement(2006..2941)
                     /locus_tag="VFH96_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974048.1"
                     /translation="MSTQTSVALRCAGLVKRFADVTAVNGLELEVFKGECFGLLGPNG
                     AGKTTTVEILEGLTPSDEGTVEILGQRWRKGDDRALRERIGVQLQETQLAEKVTVVET
                     LRLFRSFYQRGHDVDEVIHTVALEEKRNARVGKLSGGQKQRLAVACALVSDPELLFLD
                     EPTTGLDPQARLSLWDIVEKFRAGGGTILLTTHYMEEASRLCDRVAIMDHGKVIALGT
                     PADLIESLGADQIVEFSVTNELAEDGLKGLPAVTHLHKRDGDYSLTVSEIGVALPALL
                     AEIERQKSEIVTLTTHQATLEDVFVSLTGRMLRDA"
     gene            complement(3013..4668)
                     /locus_tag="VFH96_08505"
     CDS             complement(3013..4668)
                     /locus_tag="VFH96_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974049.1"
                     /translation="MSETSSKETLLNAAVLHWMHDLAAQGIVTTDSDLNVVEWNHWMF
                     DHTGKPRAEIIGRNLLELFPELTHRRLDRHYKWALEGKVSVLSQALHGYLLAMPSVSG
                     EHGFTQMQQAVRISPLSHEGRVIGTLTIIDDVTERVARESELQAQVEERSRLLSSEKL
                     ARNEAERANRLKDEFLATISHELRNPLNAILGWAHMMRLGKLNEANAERAVETIYRNA
                     KSQAQLVADLLDVSRIISGKLRLDVRTVDLISIVNAAIDSIRPAADAKSIRLQTMLDP
                     AAGPISGDADRLQQIVWNLLTNAVKFTPKGGRIQVKLQRVDSHVEIVVSDSGVGISKE
                     FLPYVFDRFRQADASTTRIHGGLGLGLSIVHQLVDLHGGSVAVQSEGEGKGATFTITL
                     PFVGVVSQKEAEAVHPTQSEEILSFDGLPSLEGLKVLVVDDEADTRELIGQVLKDCGS
                     EVIITRSAAEALEAIELHKPDILISDLGMPDEDGYSLIEKIRALPSERGGNIPAAALT
                     AYARAEDRMRVLRSGFQFHLPKPVDSAELVTVIASLAGRAYKS"
     gene            complement(4731..5348)
                     /locus_tag="VFH96_08510"
     CDS             complement(4731..5348)
                     /locus_tag="VFH96_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974050.1"
                     /translation="MKLTERQSDALSELINIAFARTGAALSELTGHRVLLSPPEVAVY
                     RTKELRDALAKFVPGDVASIHQVFAGPVAGDALLLLNYAGAVQLTDLLTDEHEPSNFL
                     DESAREVLTEVGNILLNACLGMFGNLLNVHVTFSVPRLHLETLDELLASITSNKTEIH
                     YALVVYTAFQIRDSSVKGFLVIVLSVESLDRLIQEVDAWEAQQGR"
     gene            complement(5335..5718)
                     /locus_tag="VFH96_08515"
     CDS             complement(5335..5718)
                     /locus_tag="VFH96_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6974051.1"
                     /translation="MSQKILIVDDSALSRRTLRRILESAGYEVAEAEDGMTALELYFL
                     EKPSLVLLDLVMKGMYGLDVLVKLREMDPKALVVVASADIQSSTRKLVDEAGALAFIN
                     KPFLSEPVIAAIEAALAKGVTRETN"
     gene            complement(5715..6758)
                     /locus_tag="VFH96_08520"
     CDS             complement(5715..6758)
                     /locus_tag="VFH96_08520"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET6974052.1"
                     /translation="MDRVLRVLVIDDSAYVRKVVREILSRSPFLEVVGTARDGREALD
                     LVEQLDPDVVTCDLIMPELDGVGFVREQMQRRPVPIIIMSVAKETAEAALTALDAGAI
                     DFVQKPTALASEKIFEVSNELIDKVKAAGNISLKRLPTPPDPQIAVTVESAVDGTHSV
                     DLVVLGISTGGPQALKRLIPQLPSDFPVPLVMVMHMPVGYTEMYAAKLNEISNLEVRE
                     AAEGDELKPGHVFLAPAGRHLTLKRNRDGKVVTHLDARPFSTLHRPSVDVLFQSAAEV
                     YRNRVLGVVMTGMGSDGSQGAAWIKSQGGLIFTEAESSCVVYGMPGVVVEAGLSDKSI
                     ALEDMAHAIREVV"
     gene            complement(6761..7606)
                     /locus_tag="VFH96_08525"
     CDS             complement(6761..7606)
                     /locus_tag="VFH96_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET6974053.1"
                     /translation="MRSHNLALPEGTTSLIQSLIHERTGMHFDNGKRDILMDKLSPLV
                     IERGFGSYLDYYYLLKYDPSARVEWQNVINALSVQETYFWREFDQVRALVQTLVPQWH
                     ARNAGSTMRIWCAACATGEEPLTIAIALNEARWFERASIEIVASDASTKAIDRAVGGI
                     YRERAFRNLPPALRDRYFVREGSDWRVRTDIHSRVNWGLANLMEEDQISHLADADFIF
                     CRNVFIYFSESAIGRVVRSFSRHIRPPGYLFVGAAESLIRLTTDFTLTEIDDAFVYVK
                     RERAV"
     gene            complement(7819..9975)
                     /locus_tag="VFH96_08530"
     CDS             complement(7819..9975)
                     /locus_tag="VFH96_08530"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6974054.1"
                     /translation="MTQIGVFTTDTDLVIRVWDATLAGLTGISEESATGRDLTALLPD
                     LEKRGLLKRFQRSLQEGVVEVLATAFHHYLIPCAPVNPSKHFEKMQQLVTIAPLRDDN
                     AIAGLIVTVEDVTERLERERELAARLAEGDEPARLNAAEIISDDETYDTAPLLGALSD
                     ESWRVRRAAIKGVSQRAAPDAIAALISSVVENHHNPSLLNSALQVLTSSDVDTVSPLI
                     ELLKNPNPDLRMQAALALGEQQDARATDALIEKLNDEDANVRYHAVEALGKLKATDAV
                     DALVEIAESRDFFLTFSALEALTKIGDARIVPRITPLLEDELLSIPAVNLLGQLGDES
                     TVAPLTALLNSPTGPTEAVADALATLSDRYEKQYGEARYIADLTSREVLPTGVQNLLD
                     TLEAPGKEDYRSIALVLGWLKGPGIDRALTRLMGRVDLRDEVVEALVRHGSATASLLI
                     SQLTAEDLEVRRSAVVALGRLGDTSATSALVNTLGDESLAIDAVNALGEIGDPQAVDG
                     LLNLLGNEDASIRQAAVAALNSIIPPSLSQRIIPLLHDLDPNVRESAVKIAGYFGYPE
                     ATGALVELSRDPNERVRCAAVEHLPFVEDERALAVLARALKEETPNVRAAAARALGNM
                     DGPQVVERLIEGLADDDVWVRYFSARALGRQRDEGSVEALKRVIEKDEFNHVRIAALD
                     SLGQIGGPRVAVIVAELIHDDDLDVARAAQAALERS"
     gene            complement(9972..12098)
                     /locus_tag="VFH96_08535"
     CDS             complement(9972..12098)
                     /locus_tag="VFH96_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6974055.1"
                     /translation="MSQVLAGSNNNNIERDIGLVRQQAVQVAESASQITVTANEVAEG
                     AETQLRVLDSTVAIANEMTASMGEATTQLESIATSTEELASSVNESAASIEEVTRNAE
                     SLASSINQVSAAIEESTRSIEGVSNTTQSMATSAEEVTGAMTEIAGSIRSVSADTEAL
                     TASVNETAASMEEMAASISSLSGNADDLTVSAEETSSAINEMAASIEEVAAMTEGLAS
                     SVEEVSTSIEQMARSVQGVAQNAERINDAATSAAGSANQMDRSLHAVVTLTKRAEEAT
                     TQLSKQAEEGGAAAQKSMQGILKVRTAMEQSASVIREMGKRSEEIGGIVNTINVIAER
                     TNMLSLNASIEAARAGDAGRGFAVVAEEIRNLADRSAQATADIAVIIRSLQEVVREAV
                     TAASEGTRIAEEGGQLAEEGAAGLRKILTGINQTAQLVGQIADATEEQLTAGQQVVTA
                     IGTTATQTKQVSGAVIEQAQAAQQIVKSAAQMRKTAREVSKAVGEQGRAAREIIKAAQ
                     STSGAAKQVRKSSNEQATAAKEISQAVESMRRGASSTARALSEQAVAGDQVSKEAVQL
                     ALQISEISKAVREQAKASAEISEATQSMRLQSDQVTKAMREQARTAHEMTVAVANVSK
                     DALRITNANRSHLDAADRIRSDVNQLRQITNRNADGVKATLTSTSGLADRARQLGEIM
                     DSMIGGNGAEKPKRKRTRKPAVDERG"
     gene            complement(12119..12568)
                     /locus_tag="VFH96_08540"
     CDS             complement(12119..12568)
                     /locus_tag="VFH96_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6974056.1"
                     /translation="MSLEPFILFELAGTTYGVRSSFVQQIEMIDDVASVPNAHPAVEG
                     VVLVRGQVIPALNLRTRFGFERIERELRSRLVVINTGTRVVGLVVDTAREFLKIPTES
                     IESPPEALTGLSGHYLEGIATIGERMILILNLEAVLDLDYQIEPQIS"
     gene            complement(12606..>14100)
                     /locus_tag="VFH96_08545"
     CDS             complement(12606..>14100)
                     /locus_tag="VFH96_08545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET6974057.1"
                     /translation="VETPTEPETSDSKSTRWLIEFTPTAELAERGVNVNSVRSRLQEM
                     GQLIQAKPVVKGAGEIAFEFIIANAEKSALGDLEADGLTFKPAPVESAPRAKDSQPVA
                     TIAPASVVRVDLDRLDELMRIIGELVISRTRLEDQLTELKRTTPPALWRSLQETSLSM
                     TRQLRDLRESVMRVRMVQIGEIFERMTFVVRDLARESGKKIIVQLSGGETEIDKFLVE
                     RMMDPLMHLVRNAVSHGLETVAEREAQGKRSEGLLSLSATTAGEMIVIKIADDGRGID
                     RKFVLDRASARGLSHGNADLDDAALLDLIATPGFSTRDEADRESGRGVGMDVVKKATE
                     EIGGRVSLATKVGEGTTFTIELPLTLAIAEALIVSVNGQRFAVPQSGVREVLEVESAS
                     TRVLENNEIISYRGKVLPLLRLARVFEMNYQRSEKFHVLVIGEDTNEVGLAVDRILGQ
                     REIVVRAIKDPLAQSKGIAGATELGDQRVVLILDITALRQLAVTSQH"
BASE COUNT         3055 a         4250 c         3605 g         3190 t
ORIGIN      
        1 ttactcacca cagagacaca gagaacacag aggttgcaca gagaagaagt ggtggacctg
       61 gtacgatttg aacgtacgcc ctcgaccttc gcagagtcgc gctctcattc cactgagcta
      121 caggtccaaa gtttggcgga gacggcagga gtcgaacctg cacacgcaat cgcgctacga
      181 cttagcaagc cgctgccata ccaattaggc gacgcctccg aatgatggcg gaagggacag
      241 gacttgaacc tgcaagcgac atgcgcgcgg tagttttcgg gactactgcc ttaccagtta
      301 ggctaccctt ccctgttgag agttacatct cagatttcaa atctcgctgc aaacaagatt
      361 tcaaatccga gattcacttg tgctaaaaaa tagatgaagg cagagagaga cactgcatcg
      421 aacacacttc gaggcacact tctcctgaag atggcgaact cacggactct cgcaaagcac
      481 cgagttcgtc atctgataga aggctccctg ccttcatctc tcgcaaatag gtacttcgat
      541 tatcaaaaaa ctaaaggagc gggcatttag cctcccaggg aagctgcccg ctccttcaat
      601 tctcttgatt ttcgctttga gatttgggag cgggagtccg ctcccgctga ttaaagcctg
      661 ttattccggc gattttcgga tatttggcat ccgggtctac cgccgaaaat gctggtcgtt
      721 gacgtcatga cgtctaacga gcagacgagt gaatacatgg cggcgctcgg ctgccatctc
      781 atcgtttgct ctttatgttt cctcatcgtc aacattgaaa ttcttctttc atgcggcacg
      841 cactcacgcg gcgcaagctg ttgtgtataa ggcaggacca ctaagtctgt caagcacttt
      901 cttcttatcc acagattgca cagatgtttt atggcatctg tgtaatctgt ggatgaagtt
      961 ccgtcactgc cacttgaaaa tcttcaacgc cactacaaaa ctaaccacac accatacaag
     1021 cagaatagcg atcggaatcc agtttgacgc aagcgtggcg ccttcgttca tcaaggcgcg
     1081 gagcacgttg ttgagcgcgg tcagcggtag cgcctgaatg aacggttgca ggatttgtgg
     1141 aaatcgttcc gaagagaaga acgttcccga aagcaaccac atcggcagca tgatgaaatt
     1201 catcaagccc gatacgccct cgattgtcgt cggtcgagca gcgacgagca atccgattcc
     1261 ggaaaaagta aagccaccga gtattgtcat caaagcaaca ctcagccacg agccacgcac
     1321 cgtaaaacca aacgccagcg tcgcgaagat gatgacggct gcgacttcaa gcaccagaaa
     1381 caccaaacgc gacagcatga acgaaagcaa atagtgcgaa cgccgcatcg gtgtcgcggc
     1441 aaagcgtttg aggatcttcc gcgagcgcgc gattaccacc gtaaatccga gtccccacaa
     1501 accgctgccc atcatgttca ttccgatcaa tcccggaacc agaaaatcca cataacgagc
     1561 accgggctcc gtaatcctcg cttcactaac actcaccaca tcggctcggc ccttacctct
     1621 ttgcaaaaca tcgtcaacga gaagtcgcgc agctcgactc tccggtctgg ttggatcgaa
     1681 tcgatattca aatgtcttat cacccggctt tactattatt gccactttcc cgcttcgcaa
     1741 tgctcgctgt gcttcgtcag ggctcaagac agttgcggta atctcattcg aatgagacaa
     1801 gaggctggct gtctcgctcg cctttgcgtc gccggcttcg atggcaacga gggttttatc
     1861 cggcgctgta ttgcgaaacg cgattccgag tgcgaaggtc atcaagacgg gaaagacgaa
     1921 gacccagaac acggcttctt tctcgcgcag aaactcgcgc atgcgcgtca aggtcagctc
     1981 gacgagtgga tgagattctt tcgcttcagg catcgcgcag cattctccct gtcaaactga
     2041 cgaatacatc ttcgagtgtg gcctggtgtg tagtgagtgt gacgatctct gacttttgtc
     2101 gttcgatctc ggcgagcaag gcaggcaagg cgacgccgat ctccgaaacg gtgagcgaat
     2161 agtctccgtc acgtttgtgc aaatgagtaa cggcgggaag acctttcaag ccatcctccg
     2221 caagttcgtt cgtgacacta aattctacaa tctgatcagc gccgagcgat tcgatcagat
     2281 cagccggtgt gccgagtgcg atcacttttc catggtccat gatcgcgacg cgatcgcaaa
     2341 gacgcgacgc ctcttccatg tagtgagtcg tcaaaagaat tgtgccgcca ccggcgcgaa
     2401 acttttcgac gatgtcccag agcgaaagac gtgcctgtgg atcgagtccg gtggtcggct
     2461 catccagaaa cagcagctcc ggatcgctta cgagggcaca ggcaactgcg agtcgttgtt
     2521 tctgaccgcc cgagagcttg ccgacgcgcg cgttacgttt ctcttccagc gccacggtat
     2581 gaataacttc atcaacatcg tgaccccgtt ggtagaagct gcgaaagagt cgcaacgtct
     2641 cgacaaccgt cactttctcg gcgagctgcg tctcttgtaa ctgaacgccg atacgctcgc
     2701 gtaaggcgcg gtcatcacct tttctccagc gctgtccgag tatctcaacc gtcccttcat
     2761 ccgaaggcgt caatccctcc aatatctcga ccgtcgtagt cttcccggcc ccatttggcc
     2821 caagcagccc aaaacactcc cctttgaaca cctccagctc gagcccattg acagccgtca
     2881 catccgcgaa cctcttcacg agccccgcac accgcagcgc caccgatgtt tgcgttgaca
     2941 taagctctgg atattgccac gaaaaggcac ataaagcaca gagatcagcc ctatttttgt
     3001 gctttttgtg gcttaactct tgtacgcgcg gccggcgagg ctggcgatga cggttacgag
     3061 ctcggcggag tcaacgggtt ttgggaggtg gaattggaag ccggagcgga ggacacgcat
     3121 gcgatcttcg gcgcgtgcat aggccgtgag ggctgcggcc gggatgttgc cgccgcgctc
     3181 cgagggcagg gcgcggattt tttcgatcaa tgaatagcca tcctcgtcgg gcattcccag
     3241 atcgctgatg agaatgtcag gcttgtgtag ctcgattgcc tctaacgctt cggcagcgga
     3301 acgggtgatt atcacttccg agccgcagtc cttcagcacc tggccgatca attcgcgtgt
     3361 gtcggcctcg tcatcgacga cgagcacctt gagaccttcc agcgaaggca aaccgtcaaa
     3421 cgaaaggatc tcctcgctct gagtcggatg aactgcctct gcttccttct gactaaccac
     3481 gccaacgaac ggcaacgtga tcgtaaacgt ggctcccttc ccttcgcctt cactctgcac
     3541 cgccacactg ccgccgtgaa gatccaccag ttgatgaacg atcgaaagcc cgagcccgag
     3601 gccgccatgt atgcgcgtgg tgctcgcatc tgcctggcgg aagcgatcaa acacgtaagg
     3661 aagaaactct ttgctgatgc ctacacctga atcactcacg acgatctcga cgtgcgaatc
     3721 gacgcgctgc agcttgactt gtatccgacc gcctttcggt gtgaacttaa ccgcattggt
     3781 caacaggttc cagacaatct gttgcagtcg atcggcatcg ccggaaatcg gaccagccgc
     3841 aggatccagc atcgtttgta agcgaatgct tttagcgtca gccgccggac gaatcgaatc
     3901 gatcgccgca ttgacgatcg aaatcagatc aactgtgcgt acatcgagtc gcaacttgcc
     3961 cgagatgatg cgcgacacgt cgaggagatc ggcgactaac tgggcttgcg acttcgcatt
     4021 ccggtagatc gtttcgaccg cgcgctcggc gttggcctcg ttgagtttgc cgagacgcat
     4081 catgtgggcc cagcccagga tcgcattcaa cggattgcgt aactcgtgcg agatcgtggc
     4141 gagaaactcg tctttcaaac ggttcgctcg ctccgcttcg ttccgcgcta acttctcact
     4201 tgatagcagc cgcgatcgct cttcgacttg cgcttgcaac tcagattcac gcgctacccg
     4261 ttcggtcacg tcatcgataa tcgtcagcgt tccgatcact cggccttcgt gactcagcgg
     4321 agaaattcgc accgcctgtt gcatctgcgt gaagccgtgt tcgcccgaca cggaaggcat
     4381 tgcgagcaaa taaccatgca gcgcctgcga caacacgctt acctttcctt caagcgccca
     4441 tttgtaatgt cgatcgagac ggcggtgagt cagctccggg aacagctcga gcagattgcg
     4501 gccgattatt tcagcccggg gtttcccggt gtggtcgaac atccagtgat tccattcgac
     4561 gacgtttaaa tcgctatcag tcgtgacgat cccctgcgcc gccagatcgt gcatccagtg
     4621 caggaccgca gcgttgagca atgtttcttt ggagctcgtt tctgacatat agcgggaacg
     4681 cggattttac ggatagatcc ggatttatcc gtaaaatccg cggttaaaaa tcaccggccc
     4741 tgttgcgctt cccaggcgtc gacttcctgg attaaacgat cgagcgactc aacgctcaac
     4801 acgattacca gaaatccctt gactgaactg tcgcggattt gaaaggccgt gtagaccacc
     4861 agcgcgtaat gaatctcggt tttatttgac gttatagacg caagcaactc atcgagcgtc
     4921 tcgagatgca gccgcggcac ggaaaacgtg acgtgaacgt tcagcaaatt tccaaacatt
     4981 cccagacacg cattcaaaag aatattccca acctccgtta aaacttcacg cgctgattcg
     5041 tcgagaaagt ttgacggctc gtgctcgtcg gtgagcagat ccgtgagctg tacagcccct
     5101 gcataattca ataacaacaa cgcgtcaccg gccactggtc cggcaaacac ctgatgaatc
     5161 gaagccacat cgccgggaac aaatttggcc agcgcgtcgc gcaactcttt agttctataa
     5221 accgccacct ccggcggact cagcaacaca cgatggcccg tcagctccga gagcgccgct
     5281 cccgtgcgcg cgaaggcaat gttgatgagc tcggaaagcg cgtcgctctg tcgctcagtt
     5341 agtttcacga gtgactccct tcgcaagtgc tgcctcgatc gctgcaatca ccggttccga
     5401 cagaaacggt ttgttgatga aagcgagcgc tcccgcttca tccaccaatt tccgggtcga
     5461 actctgaata tcagcgctgg caactacgac taatgccttc ggatccatct cgcgcaactt
     5521 cacgagcaca tcaagcccgt acataccctt catcaccaga tcgagcaaca ctaaactcgg
     5581 cttttcgagg aagtagagct caagcgcagt cattccatct tctgcttccg ccacttcgta
     5641 accggcggat tcaaggattc gtcgcaacgt cctgcgtgac aacgctgaat catcgacaat
     5701 cagaatcttc tgactcatac aacctccctg atcgcgtggg ccatgtcttc gagagcgatg
     5761 ctcttgtcac tcaaaccggc ttccaccacg acgcctggca taccatacac cacgcaactg
     5821 ctctccgctt ctgtaaagat cagcccgccc tgcgatttga tccaggcggc tccctggctt
     5881 ccatccgatc ccatccccgt cattacaacc ccgagcacgc gattgcgata gacttctgcc
     5941 gcggattgaa ataacacgtc aaccgatgga cgatgcagcg tactaaacgg tctggcatcg
     6001 aggtgggtaa caaccttccc gtctctattt cgtttcaatg tcaggtgacg acccgcgggc
     6061 gcaaggaaga catgacctgg tttcaactcg tctccctccg ctgcttctcg cacttcaaga
     6121 ttagatatct cgttcaactt cgctgcgtac atctcggtgt aaccaaccgg catgtgcatc
     6181 accatcacga gcggcaccgg aaaatctgac ggcaactgcg gtatcaatcg tttgagcgcc
     6241 tgtggtccac cagtcgagat accaagcacg acgaggtcaa ctgagtgcgt cccgtcaacc
     6301 gccgactcga ctgtcactgc gatctgcggg tccggcggtg tgggaagccg tttcagagaa
     6361 atgttgcctg ctgctttgac cttgtcgatc aactcattac tgacttcaaa gatcttttca
     6421 ctcgccagcg ccgtcggttt ctgaacgaag tcgatcgctc cagcgtcaag cgcggttaaa
     6481 gcagcttcag ccgtttcctt cgcgacgctc atgataatga tcggcacagg ccgccgctgc
     6541 atttgttcgc gcacgaatcc gacgccgtcc agctcgggca tgatgagatc acacgtaact
     6601 acgtccggat cgagttgttc gaccagatcg agtgcttctc gaccgtcgcg cgccgtgccg
     6661 acaacttcga ggaaagggct gcgagagagt atctctcgca cgaccttccg cacgtatgca
     6721 gaatcgtcga tcaccaatac acgtaaaact cgatccatcg tcagacggcc ctctcacgct
     6781 ttacatacac gaaagcgtcg tcaatttcgg tcagggtaaa atcggtcgtc aatcgtatta
     6841 gtgattcggc ggcgcctaca aatagataac ccggaggcct gatatgtctc gagaaagaac
     6901 gaaccacgcg gccaatcgcg ctttctgaga agtaaataaa tacgttgcga cagaaaataa
     6961 aatccgcgtc ggcgaggtgg gagatctggt cttcctccat taagtttgca agtccccaat
     7021 taactcgtga atgtatgtcg gttctgacac gccagtctga accttctcgt acgaaatagc
     7081 gatcccgcag agcgggtgga agattgcgga aggcgcgctc tcgataaatg cctccgacgg
     7141 cgcgatcgat ggcctttgtg ctggcgtcac tggctacgat ctcgatcgac gcgcgttcaa
     7201 accatcgggc ttcgttcaag gcaatcgcga tcgtcaacgg ttcctcgccg gtggcacaag
     7261 cggcgcacca gatccgcata gttgagcccg cgtttcgagc gtgccactga ggtactaatg
     7321 tttggaccag cgcgcgcact tgatcgaact cgcgccagaa gtaggtctcc tgcaccgaga
     7381 gtgcgttgat cacgttctgc cattcgacgc gcgctgacgg atcgtatttc agcaagtagt
     7441 aatagtcgag atacgaaccg aaaccgcgct cgatgacgag tggcgagagt ttgtccatga
     7501 gaatgtcgcg cttgccgttg tcgaaatgca ttccggtgcg ttcgtggatc agactttgaa
     7561 tcagactcgt ggtgccctca ggtaatgcca gattatgtga acgcaacgac accatagttc
     7621 gaatgatttc agcccgcgaa cgcgggcggc agcataaagc ctggggcgca agccccagga
     7681 tcaccgtaaa aaaattcgcg ttgagcccgc ggagcgggcg gcagcgctgt cgcccgcgtt
     7741 cgcgggctcg cgctctaata actaacaaag atcctggggc tcgcgcccca ggctttatgc
     7801 ttacgcctgc ttcgcaggct aagacctttc aagcgcagcc tgcgcggcgc gggcgacgtc
     7861 gagatcgtcg tcgtgaatca actccgcaac aattaccgcg acacgcggac caccgatttg
     7921 tcccagcgaa tcaagggccg caatgcgaac gtgattgaat tcgtctttct caatcactcg
     7981 cttgagagcc tcgacgcttc cctcgtcccg ttgacgaccc aacgcgcgcg cgctgaagta
     8041 gcgtacccaa acgtcgtcat ctgccagtcc ttcaatcaat cgctcgacaa cttgcggccc
     8101 gtccatattg ccgagcgcac gagccgcagc cgcacgcaca ttcggcgttt cctctttcaa
     8161 tgccctggca agaacagcta acgcacgttc gtcttcaaca aacggcaggt gctcaaccgc
     8221 cgcacaccga actcgctcgt tcggatcgcg actcagctcc accagggcgc ccgtagcctc
     8281 cgggtatccg aagtacccgg caatcttcac tgccgactcg cgaacgttcg gatccaggtc
     8341 atgaagaagc ggaatgatac gttgcgacag cgacggcgga atgatcgaat tcagcgccgc
     8401 tactgccgcc tgcctgatcg atgcatcctc gttgccgagc aaattcagta gtccatcgac
     8461 tgcctgtgga tcgccgattt caccgagtgc gttcacagca tcgatcgcca gagactcgtc
     8521 accgagtgta tttacaagcg cggaggttgc gcttgtatcg ccgaggcgtc cgagcgcaac
     8581 caccgccgag cgtctcactt ccaaatcttc cgccgtcaac tgtgaaatca atagactggc
     8641 cgtcgcagag ccatgccgca cgagcgcttc gacaacctca tcgcgcagat cgacgcgtcc
     8701 catcaatcgc gtgagcgcgc gatcgatacc gggacctttg agccagccaa gcaccaacgc
     8761 gatcgagcga tagtcttctt taccgggcgc ctccaacgta tcgagcagat tctgaacgcc
     8821 ggtcggcagg acttcacggc tggttagatc cgcgatgtaa cgagcttcgc catattgctt
     8881 ctcatatcga tcgctgagag tggcgagcgc gtccgcgacc gcctccgtgg gaccagtcgg
     8941 actattcaaa agagcagtaa gaggagcgac tgtactttca tcgcccagtt ggcccagcag
     9001 gttgactgct ggtatagaca aaagctcgtc ttccaaaagc ggtgttattc tgggcacgat
     9061 gcgcgcatcg ccgatctttg tgagcgcctc caatgccgaa aacgtaagga agaagtctct
     9121 tgattccgct atctcaacca gtgcatcgac ggcatctgta gccttcaact tacccagcgc
     9181 ctcaaccgcg tgataacgca cgttggcgtc ttcgtcattt agtttttcta tcaacgcgtc
     9241 agttgcacgt gcgtcttgtt gctcacccaa agccaacgct gcctgcatgc gcagatctgg
     9301 attcgggttt ttcagcagct cgatcagggg cgaaacagtg tcgacatcac tcgaggtcaa
     9361 gacctgtaac gcactgttca gcagcgaagg attgtgatgg ttctcgacta ccgagcttat
     9421 cagcgctgcg atcgcatcag gcgcagcacg ttgcgacact cctttgatcg cggcgcgacg
     9481 aactctccaa ctctcatcac tcaacgcacc aagcaacggt gccgtgtcgt aagtttcatc
     9541 atcgctaata atctcagccg cattcagccg cgccggctca tcaccttcag ccaaacgcgc
     9601 cgcaagctcg cgctcacgct caagccgctc agtaacgtct tcaacagtga caatgaggcc
     9661 cgcaatcgcg ttgtcgtcgc gaagcggcgc aatcgtcacc agttgctgca tcttctcgaa
     9721 gtgtttagag ggattaactg gagcacaagg gatcagataa tgatgaaagg cagtggcgag
     9781 cacctccact acgccttcct gcaacgatcg ctggaaacgt ttcaacagcc cacgcttctc
     9841 gagatcgggc agcagcgcag tcagatcgcg gcccgtagca gattcctcac tgataccggt
     9901 aagccccgcg agcgtcgcat cccaaactcg aattacgaga tcggtgtctg ttgtgaaaac
     9961 gccgatctgc gtcatccacg ttcatccact gccggttttc tcgtacgctt gcgtttaggc
    10021 ttctcagcgc cgttgccacc aatcatgctg tccatgatct caccgagttg gcgcgcgcga
    10081 tcagccaaac cggacgtgct ggtcagcgtt gctttaacac catccgcatt gcgatttgtt
    10141 atctgccgca actggtttac atcgctgcga atccgatcag cagcatcgag atggctgcga
    10201 ttcgcattcg taatccgcag cgcgtctttc gaaacgtttg ccacagcgac agtcatctca
    10261 tgcgccgttc gcgcctgctc gcgcatcgcc ttcgttactt gatcggactg caaacgcata
    10321 ctctgcgtcg cctctgaaat ctctgcagac gctttcgcct gctcacgcac cgctttgctg
    10381 atctcactga tctgtagcgc gagttggact gcttccttcg aaacctgatc gccggccact
    10441 gcctgttcac tcagcgcacg cgcagtcgag gaggcgcccc gacgcatcga ttcgacggct
    10501 tgagagatct ccttggcagc cgtggcttgt tcgttcgaag acttgcgcac ctgcttagcc
    10561 gcgccgctcg tgctctgcgc cgccttgatg atctccctcg cagcgcggcc ttgctcgccc
    10621 acagccttgc tgacttccct ggcagttttc cgcatctgcg ctgctgactt gacgatctgt
    10681 tgcgcggcct gggcctgttc gatcacagcg ccggaaactt gttttgtttg cgtcgcagtc
    10741 gtgccgattg cggtgaccac ctgttgacct gctgtcaatt gttcctcggt tgcatccgca
    10801 atctgtccga cgagctgtgc ggtttgattg atgccggtca gtatcttccg tagacccgcc
    10861 gcgccttctt ccgcgagttg accaccctcc tccgcgatac gcgttccctc gctcgcagca
    10921 gtcaccgctt ctctgaccac ttcctgaagc gacctgatga tcactgcgat atcggctgtc
    10981 gcctgcgcgg atcgatcggc gagatttctg atctcttccg caacgacagc aaaccctcgg
    11041 cccgcatcac cggcgcgcgc cgcctcgatc gaagcgttga gcgaaagcat attggtgcgc
    11101 tcggcgatga catttatcgt gttgacgatg ccgccgatct cttcagaacg ttttcccatc
    11161 tcacggatga cgctcgctga ttgctccatc gccgtacgga ctttcagtat tccctgcatc
    11221 gacttctgcg ctgcggctcc gccctcttcg gcttgtttgc tcaactgcgt agtggcttct
    11281 tcggcacgct tcgtcaacgt cacaaccgca tgtaacgaac ggtccatctg attagcgctg
    11341 ccggccgcac tcgtcgcggc gtcattaatg cgctctgcat tctgcgcgac tccctgaact
    11401 gagcgcgcca tctgctcgat cgaagtcgac acttcttcaa ctgacgaagc gagaccttcg
    11461 gtcatcgctg caacctcttc gatcgacgcc gccatctcgt tgatggctga agaagtctcc
    11521 tccgcggaga cggtgagatc gtcggcattt ccggagagac tggagatcga ggctgccatc
    11581 tcttccatgc tggccgcagt ttcgttcacc gaagcggtga gtgcctccgt gtcggcactg
    11641 acagatctga tcgaaccggc gatctccgtc atcgcaccgg taacttcttc agccgatgtc
    11701 gccatgcttt gcgtcgtgtt gctgacgcct tcaatcgaac gcgtgctttc ctcgatggca
    11761 gcggagacct gattgatcga tgaggcaaga gattcggcat tcctggtgac ctcttcgatt
    11821 gatgcggcac tctcgttaac cgacgacgcg agctcttcag ttgaagtagc aatcgactcg
    11881 agctgagtag tcgcctcgcc catggacgca gtcatctcat tagcgatcgc gacggtgctg
    11941 tcgagtacgc gcagctgtgt ttcggcgcct tcagccacct cgttggccgt tactgtgatt
    12001 tgactggcgg attcggccac ctgcactgct tgttggcgaa ccagtccgat gtctctctcg
    12061 atgttgttgt tgtttgagcc agctaggacc tgagacatag tcgtatctcc gtatttgctt
    12121 atgaaatctg tggctcgatc tggtaatcaa gatcgagcac ggcttctaaa ttcaaaatca
    12181 agatcatgcg ctcgccaatc gtggcgatgc cttcaaggta gtgaccactc aatccggtaa
    12241 gcgcttcggg cggcgactcg atcgactccg ttggaatctt gagaaactcg cgcgccgtat
    12301 cgacgacgag tccgaccacg cgcgttccgg tgttgataac aaccaatcgc gaccgcagct
    12361 cgcgttcaat tctttcgaaa ccaaaacggg tgcgcaaatt caacgccgga atcacctgcc
    12421 cgcgaaccag cacgactcct tcgaccgcgg gatgagcatt tggcactgac gcaacgtcgt
    12481 cgatcatctc gatctgttgc acaaagctac tgcggacgcc ataagtcgtg cccgcgagct
    12541 cgaagagtat gaatggttct aagctcatgt ctttaatccg tgtgattatc cgtgcaaatc
    12601 cgtggctaat gttgtgacgt taccgcaagc tgtcgtaggg cagtgatgtc taaaatcaat
    12661 accacgcgct gatcgccgag ctcggtagct cccgcgatgc ctttgctttg cgcgagcgga
    12721 tccttaatgg cgcggacgac gatctctcgc tgtccgagta ttcgatcgac cgcgagaccg
    12781 acttcgtttg tatcttcacc aatcaccagc acgtgaaact tctcgctgcg ctggtaattc
    12841 atttcaaaga cccgcgcgag acgaagcagc ggcaggactt tgccgcgata ggaaatgatt
    12901 tcgttatttt ccaacactct cgtcgacgct gactcaacct ccaacacttc cctcacgccc
    12961 gactgtggca ccgcaaagcg ttgaccattc acgctcacga tcagtgcttc cgcgatcgcg
    13021 agtgtcaaag gcaactcgat cgtgaacgta gttccctcac cgacttttgt cgccagcgaa
    13081 actcggccgc cgatctcttc ggtcgctttc ttgactacat ccattcccac gccacgcccg
    13141 ctttctcgat cggcttcgtc ccgtgttgaa aatccgggag tcgcgatcaa atcgagtaag
    13201 gccgcgtcat cgagatcagc attgccatga ctgagaccgc gggcactggc tctatcgagg
    13261 acgaactttc ggtcgatgcc gcggccgtcg tccgcgatct tgatcacgat catttcaccg
    13321 gcagtcgtcg cgctcagtga taacaaacct tcacttcgct ttccttgtgc ttcacgctca
    13381 gcgacagttt caagtccatg actgacggca ttgcggacga gatgcatgag cggatccatc
    13441 atccgctcga cgagaaactt gtcgatctcc gtttcgccgc cgctgagctg cacgatgatc
    13501 ttcttaccgc tctcacgcgc cagatcgcgc acgacgaacg tcatgcgctc gaagatctcg
    13561 ccgatctgga ccatgcgcac gcgcatgacg ctctcgcgca aatcgcgcaa ttgccgcgtc
    13621 atgctcaggc tggtttcctg tagcgaacgc cagagagcgg gcggtgtcgt gcgcttcagt
    13681 tccgtcagtt gatcctcgag ccgcgtgcga ctgatcacga gctcaccgat gatgcgcatc
    13741 agttcgtcga gacgatcgag atcgacgcgc acgacactcg caggcgcgat cgtcgctact
    13801 ggctggctat ctttcgctcg cggcgcggat tcaaccggcg caggcttgaa cgtcaaacca
    13861 tcggcttcga gatctccgag cgccgacttc tctgcgttgg cgataataaa ttcgaacgcg
    13921 atttcgcccg cgcctttaac caccggcttc gcctggatca actgtcccat ctcttgcagc
    13981 cgtgagcgca cgctgtttac gttgacgcca cgctcggcaa gttcggcagt cggtgtgaat
    14041 tcgatcaacc agcgcgtcga cttgctgtca gatgtttcgg gttctgttgg tgtttcgacc
//
LOCUS       DASSFQ010000234         6611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_851978, whole genome shotgun sequence.
ACCESSION   DASSFQ010000234 DASSFQ010000000
VERSION     DASSFQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851978"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1388)
                     /locus_tag="VFH96_08550"
     CDS             complement(51..1388)
                     /locus_tag="VFH96_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6974058.1"
                     /translation="MINPANSENSAILSKGHLLRVLGVGFGVAVIIGNTIGAGIFRAP
                     GTIAEQLPSTVPFLLVWLAGGLYALLGAISLAELGTMLPRSGGQYVFSRYALGEYAGF
                     IVGWSDWISSCGSTAAVSLLTATFAVALFPSLDGHAAAVAAGIATIFAFLQWRGIVWG
                     SNIQNLTSLLKALAFLALIVAAFLLGGRGALSTPQIHSGPITLAAIVVSLQAAIYTYD
                     GWSGVIYFSEEVKDPNRNIPRALFNGVLTITVIYVLVNIALLYVLPMSRIAGKEFAAG
                     EAANVIFGARGDTVFRCLTIVSMLSAINALHLMATRVIFAMSRDGLVSQKAAVVNEGG
                     TPTIALALSLIVSLLFIIFGEKFETVITVLAFFFVVNYILSFVSLFVLRRREPDKPRP
                     YRAWGYPITPALALIGSVLFLAGAIRADTRNSVYALVLLAVSYPVYRLMSRAS"
     gene            complement(1381..2505)
                     /locus_tag="VFH96_08555"
     CDS             complement(1381..2505)
                     /locus_tag="VFH96_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET6974059.1"
                     /translation="MNDQLKTIIEQYDPNLELDYALTIPSSWYIDKGFYDHELQTVFS
                     QSWQLAARADQLTEPGQYVTTDIAGEPVVIVRGNDGELRGFFNVCRHHAAAVMTAPEG
                     KAAQLRCPYHGWTYSLAGELKGTPDFSGVCDFDRAKNGLVSLEIAEWENWVFVKLVQE
                     LAISIEDFLGEDLVAQTSGLQLKDLHWFERRHYHFDCNWKVFVDNYLDGGYHVPYLHK
                     GLDSVLDYSNYMIENGEHYCLQWSPIVSEGAEEQTGAVRKGDRALYYWIYPNFMINWY
                     DGVMDTNLVVPRGVDQTEVIFDFYFPDVMSEEMCRASVEVGQRIQDEDVAICKSVQRG
                     LNSRAYDAGRLSVRREAGEHLFHRLLYRDVCLTGFQDLHD"
     gene            complement(2502..3704)
                     /locus_tag="VFH96_08560"
     CDS             complement(2502..3704)
                     /locus_tag="VFH96_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HET6974060.1"
                     /translation="MPIGTAFHERTFPLCESLSYREWSGYYAVSSYEPHHEHEYNAIR
                     NAAALIDISPLFKYRLTGKDATRLVDRVITRDVRKVSVGQVVYTPWCDERGKVIDDGT
                     VSRLAENVYRWTAADPSLRWFTQNAAGMDVQIEDISESVSALALQGPTSGRLLKTLVE
                     DADIANLKYFRVTKGAIAGVPVEISRTGYTGDLGYEIWIESEKAIRVWDALMETGRAF
                     DIHPAGMLALDVARVEAGLLLIDVDFNSSKKALVDEQRYSPFEMGLGRLVNLDKNRFV
                     GQQALIEEQKHGHPREIVGLQIDWPQVESLYEKVGLPPAVSPIASRVAVPVFKEGIQV
                     GKATTTTWSPTLKSMIALATVKREYTRPGTRLQFEITVEAVRHQVRATVAKTPFFNPK
                     RKTATPVV"
     gene            3850..4083
                     /locus_tag="VFH96_08565"
     CDS             3850..4083
                     /locus_tag="VFH96_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974061.1"
                     /translation="MSTSSEELLKSFDLLPEVEKREVASEIIRRTFSLGREPAMDEAQ
                     MRSLYASFADEDHELAEDGMEDYDKGLAIEDAQ"
     gene            4300..5877
                     /locus_tag="VFH96_08570"
     CDS             4300..5877
                     /locus_tag="VFH96_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6974062.1"
                     /translation="MQSKFDVVVIGGGHNGLVNAAYLARAGKKVLVLERRHVLGGAAV
                     TEEVFPGFKFSVCSYVVSLLRPEIIRELDLPRHGLEILPLDGTFTPMPNGDYLWRVND
                     HARTRREIARHSRLDAEAYDEYGKAMIEMAHFVKPIMSMTPPDPTSLDPKAWKNLAFL
                     GRRFQKLSDEDKYNQVQLMTMSAVDFLDQWFETDVLKATMSASGIIGTFLGVRSPGTA
                     YVLLHHYMGEIDGAFRSWGFARGGTGAISNSIADAACEAGVEIRTSAPIAKILHKNGR
                     ATGVVLENGDEILADVVSSSVDPRLTFMKMIGEQHLPDDFCTDLKRYKYRGSSGKVNL
                     SLDGLPDFKSLPGPGAHLRGAISISPSVDYMERAYDEAKYGRFSRRPYIDMVIPSLTD
                     PSVAPPGKHVMSCFVQYAPYNLKEGNWDEKREEFGNAVIETIAEHAPNIKSLIRDRQV
                     LTPLDIERDFGLSEGNIFQGELTLEQLFFLRPAPGWAQYRTPIKNLYMCGSATHPGGG
                     IMGASGRNAALQMLKDI"
     gene            5943..>6611
                     /locus_tag="VFH96_08575"
     CDS             5943..>6611
                     /locus_tag="VFH96_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6974063.1"
                     /translation="MYDVIIIGAGHNGLVAACYLAKSGLKTLVLEGREVVGGGAVTEE
                     IHPGFRCSTLDHAATVAAQVVADLNLARHGLEMITPAARVLALSPDGRSLSIYNDVGR
                     TVSEIEKFSANDARSYPEFVNSFSRIGRVLAPLLSMTPPSIDQPSATDLWHLGKVGLA
                     FRGLGKKDEYRLLRWAPMAVADLVSEWFETELLRATVAARGIHGAFAGPWSAGTSLGL
                     LWQAA"
BASE COUNT         1492 a         1893 c         1822 g         1404 t
ORIGIN      
        1 actagataat accggttgac ccggcgctac cgcgctcggt tctgtgcata ctacgacgct
       61 ctactcatta atctgtagac gggataactc accgccaaca acacgagcgc gtaaacacta
      121 tttctcgtat cagcacgtat cgctccggcg agaaacaaca ccgatccaat caacgcgagc
      181 gccggcgtta tcggataacc ccaagcccga tacggacgcg gtttgtcagg ctcacgccgg
      241 cgcaacacga acagcgaaac gaacgagagt atgtaattca ccacgaagaa gaacgcgagc
      301 accgtgatga ccgtctcaaa cttctcgccg aagatgatga atagcagcga aacgatcaaa
      361 ctcaaggcga gggcgattgt cggcgtgccg ccttcgttaa caaccgcagc cttctgcgaa
      421 accagtccat cgcgactcat cgcaaagatc acgcgcgtcg ccatcaagtg aagcgcgttg
      481 atcgcgctca gcatcgacac gatcgtcagg caacgaaaga ccgtgtctcc gcgggctccg
      541 aagataacgt tcgccgcttc gccggccgcg aattccttgc ccgcgattcg cgacatcggc
      601 aacacgtaaa gcaacgcgat gtttacgagc acatagatca cggtgatcgt gagcacgccg
      661 ttgaacagcg cgcgcggaat gttgcggttt gggtctttaa cttcctcgga aaaatagatg
      721 actccgctcc aaccgtcgta ggtgtaaatc gcggcctgca acgagacgac gatggcagcg
      781 agcgtgatcg gacccgaatg aatttgcggc gtcgataacg cgccgcggcc gccaagcagg
      841 aacgcggcaa cgatcagcgc cagaaacgca agtgctttga ggaggctggt gaggttttgg
      901 atattgctgc cccaaacaat tccgcgccac tgcagaaacg cgaagatcgt cgcaatgccg
      961 gcggcaaccg cagcagcgtg tccgtcgagc gatgggaata gtgcaactgc aaacgtcgcg
     1021 gtcagcagcg aaactgccgc cgtcgatcca catgacgaga tccaatcact ccagccgaca
     1081 atgaacccgg catactcgcc gagggcataa cgcgagaaca catactgccc accagagcgc
     1141 ggcagcatcg taccgagctc cgcgagtgag atcgcaccca gcagtgcata tagcccacca
     1201 gccagccaaa cgagaagaaa cggaactgta gacggaagtt gctcggcgat cgtgccgggc
     1261 gctctgaaga tgcctgcacc gatcgtattg ccgatgatta cagcgacacc gaagccaacc
     1321 ccgagcacgc gcagcagatg gcctttagac aggattgcag aattttcaga atttgcagga
     1381 ttaatcatgt aaatcctgaa atcctgtcaa acatacatcc ctatacagca gccggtgaaa
     1441 caagtgctcg ccagcctctc gtctcaccga caatctcccg gcatcgtacg ccctcgaatt
     1501 caacccacgc tgcactgact tacatatcgc gacatcttca tcctggatac gctggcccac
     1561 ctcgacgctc gccctgcaca tctcctcact catcacatcc ggaaagtaaa aatcaaagat
     1621 cacctcggtc tggtccacac cgcgcggcac gaccaggttc gtatccatca cgccgtcgta
     1681 ccagttgatc atgaagttcg gataaatcca gtagtacagc gcacgatcgc cttttctgac
     1741 cgcgcccgtc tgctcctccg cgccctcgct aacgatcggg ctccactgta aacagtagtg
     1801 ctcgccgttt tcgatcatgt aattgctgta atcgagcacg ctgtcgagcc ccttgtgcaa
     1861 atacggcacg tgataaccac cgtcgagata gttatcgaca aacactttcc agttgcaatc
     1921 aaaatgataa tgacgccgct cgaaccagtg cagatctttc aactgcagcc cgctcgtctg
     1981 cgccaccaga tcctcaccaa gaaaatcctc gatcgagatc gcgagttcct ggaccagctt
     2041 cacaaatacc cagttctccc actccgcgat ctccagcgac acgagaccat tcttcgcgcg
     2101 atcgaaatcg cacacacctg aaaaatccgg cgtgcctttc agctcacctg cgagcgagta
     2161 agtccaaccg tgatacgggc aacgaagctg cgccgcttta ccctccggcg cggtcatcac
     2221 tgccgccgcg tgatgccggc aaacgttgaa aaatccgcgc agctcgccgt catttccgcg
     2281 cacgatcacc accggctccc ccgcgatgtc agtggtgacg tactggcccg gttcagtcaa
     2341 ctgatctgcg cgcgccgcga gttgccatga ttgcgagaag acggtttgaa gctcgtgatc
     2401 gtagaagcct ttatcgatgt accacgagga aggaatcgtc aacgcataat cgagctcgag
     2461 gttcggatcg tattgctcga tgatcgtctt caattggtcg ttcatacaac cggcgttgct
     2521 gttttgcgtt tgggattgaa gaatggcgtc ttcgccaccg tggcgcgcac ctgatgcctg
     2581 acggcctcga ccgtgatctc gaattgcagg cgcgtgcccg ggcgtgtgta ctcgcgcttc
     2641 accgtagcga gcgcgatcat cgacttcaac gttggcgacc aggtggtcgt tgtcgctttg
     2701 ccaacttgaa tgccttcctt gaacaccggc acagcgactc gcgatgcgat cggtgatacc
     2761 gctggcggca gtccgacttt ttcatagaga ctctcgacct gcggccagtc gatttgcagg
     2821 ccaacaatct cgcgcgggtg cccgtgcttc tgctcttcga tcaaagcctg ttggcccacg
     2881 aagcgattct tatcgagatt gacgaggcgt ccgaggccca tctcgaaggg tgagtagcgc
     2941 tgttcgtcga cgagcgcctt cttgctgctg ttgaaatcca cgtcgatcaa cagcaatccg
     3001 gcttcaacgc gcgcgacatc gagcgcgagc attcccgcgg gatgaatatc gaacgcgcga
     3061 cccgtttcca tgagcgcgtc ccaaaccctg attgctttct ctgattcaat ccagatctca
     3121 taaccgagat cgcccgtgta gcccgtgcgc gagatctcaa ctggcacgcc cgcgatcgca
     3181 cctttagtta cacgaaaata tttgaggttc gcgatgtccg catcttccac gagagtcttc
     3241 agcaggcggc ctgaagtcgg accttgcaga gcgagtgctg acacgctttc ggagatatct
     3301 tcgatctgca catccatccc ggcggcgttt tgcgtaaacc atcgcagact cggatcggca
     3361 gcggtccagc gatacacgtt ctctgcgagc cgcgaaacgg tgccatcatc gatcaccttc
     3421 ccgcgctcat cgcaccacgg cgtgtagacc acctgaccca cagacacttt gcgcacatcg
     3481 cgcgtgatca cacgatcgac gagccgcgtc gcgtccttgc cggtgagccg gtatttaaac
     3541 agcggcgaaa tatcaatcag tgccgcggca ttccgaatcg cattgtattc atgctcgtga
     3601 tgtggctcat acgagctgac ggcataatac ccagaccact cgcgatagct aagactctcg
     3661 cagagcggga atgtccgttc atgaaacgca gttccgatag gcatctaagc ttgttttttg
     3721 ccgcggatta acgcgaatta acgcagatca gaatgtacct tatttctgat ccgcgtttat
     3781 ctgtgttcat ccgcggctgg tctttttccg gtgtttgtca gggcgagcga ttataagtat
     3841 cctaacgcaa tgagtacaag tagcgaagaa cttctaaaat cgttcgatct tctcccagag
     3901 gttgaaaagc gcgaagtggc ttccgagatt attcgtcgaa cgttttcgct tggtcgcgaa
     3961 ccagccatgg atgaagctca aatgaggagt ctttacgcta gctttgccga tgaagatcac
     4021 gagttggctg aggatgggat ggaggattac gacaaaggtc tggctataga ggatgctcaa
     4081 tgacggtcag gcgcggagag atttggctcg cgaactttaa tccaatacgc ggttcagaac
     4141 aggcaggtac tcgtccggtc ttgatttttc agaatgatgc tataaacaga ttcacaacga
     4201 cctttctttc aattccactg acaaagaaag aaacgctcac agagattgag agtgtcgtga
     4261 tgttcactac cgggattgtg taacccgggc tcaaacagca tgcaaagcaa attcgacgtc
     4321 gtcgtgatcg ggggcgggca taacgggctg gttaatgccg cctatctcgc gcgtgccggg
     4381 aaaaaggtcc tcgtgctcga gcgccggcat gtgctcggtg gggcggcggt tacggaagag
     4441 gtgtttcccg ggttcaagtt ttctgtctgc tcgtatgtag tctcgctgct gagaccggag
     4501 atcattcgcg agctcgatct tccgcgccac ggtctcgaaa ttcttccgct cgacggcacc
     4561 ttcacgccga tgccaaacgg cgactacctc tggcgcgtca acgatcatgc acgcacgcgc
     4621 cgcgagatcg cgcgccactc gcgtctcgac gccgaagctt acgacgagta cggcaaggcg
     4681 atgatcgaga tggctcactt cgtgaagccg atcatgagca tgacgccccc agaccccacg
     4741 tccctggatc ccaaagcctg gaagaacctt gccttcctcg gccggcgctt tcagaaactc
     4801 tccgacgaag acaagtacaa ccaggtgcag ttgatgacca tgagcgcggt cgattttctc
     4861 gatcagtggt tcgagaccga cgtgctcaaa gcgacgatgt cggcatcggg aatcatcggc
     4921 acatttctcg gcgtgcgctc gccgggaacc gcatacgtgc tcttgcatca ctacatgggc
     4981 gagatcgacg gcgcgtttcg ctcgtggggt tttgcgcgcg gcggcaccgg cgcgatctcg
     5041 aattcgatcg ctgacgcggc gtgtgaagcc ggtgtcgaga ttcgcaccag cgcgccgatt
     5101 gcaaaaattt tgcataagaa cggccgagcg accggcgtcg tgctcgagaa tggcgatgag
     5161 atccttgccg acgtggtttc atcgagcgtc gatccgcggt tgacgttcat gaagatgatt
     5221 ggcgaacaac acctgccgga cgatttctgc acggacctca aacgctacaa gtatcgcggc
     5281 tcgtcaggga aagtgaatct ctcgctcgat ggtctgcctg atttcaagtc gctgcctggt
     5341 ccaggcgcgc acctgcgcgg cgcgatctcg atctcgccga gcgttgatta catggagcgc
     5401 gcgtacgatg aagcgaagta cggacgtttt tcgcgtcgcc cctatataga catggtgatc
     5461 ccgagtctca ccgatccttc ggtcgcgccg ccgggcaaac acgtgatgtc gtgcttcgtg
     5521 cagtacgcgc cgtacaacct gaaagaagga aactgggacg agaagcgcga ggagtttggc
     5581 aacgcggtga tcgagacgat tgccgagcat gcgccgaaca tcaagagcct gattcgcgat
     5641 cgacaggtgc tgacgccgct tgatatcgag cgcgattttg ggttgtcgga aggaaatatt
     5701 ttccagggtg agctaacgct cgaacagctc ttctttctgc ggccggcgcc gggttgggcg
     5761 cagtatcgca cgccgatcaa gaacctttac atgtgcggtt cggcgacgca tccgggcggc
     5821 ggaatcatgg gcgcgtcggg acgaaatgcg gcgttgcaga tgctgaaaga catttagccg
     5881 cggattacac ggataaaaat ccggattcaa tccgtgttat ctgtggctaa taaaactgaa
     5941 tgatgtatga cgtaatcatc atcggagctg gtcacaacgg gcttgtggct gcgtgttatc
     6001 tcgcgaagag tgggctgaag acgctcgtgc tcgaaggtcg tgaggtggtt ggtggtggtg
     6061 cggttactga agagatccac ccgggatttc gttgttcgac gttggaccac gcagcgacag
     6121 tggcggcaca agttgtcgct gatctgaatc tggcgcgaca tggattggag atgatcacgc
     6181 ctgcagcgcg tgtgcttgca ctctcgccgg atggaagatc gctgtctatc tacaacgacg
     6241 ttggtcgcac ggttagcgag atcgaaaaat tttccgcaaa cgacgccaga agttatcccg
     6301 agttcgtcaa cagcttctca cgcattggcc gcgtgttggc gccgctgctt tcgatgacgc
     6361 caccgtctat tgatcaacca tcggcgacgg atttatggca tctgggtaaa gtcgggctcg
     6421 cgtttcgtgg tctcggcaag aaagacgagt atcgactact gcgctgggct ccgatggcgg
     6481 tggcagatct cgtcagtgaa tggtttgaaa ccgagttgtt gcgcgcgacg gtcgccgcca
     6541 ggggaattca cggagcgttt gctggtccat ggtctgcggg tacgagtctc ggactgttat
     6601 ggcaggcggc g
//
LOCUS       DASSFQ010000235         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2198861, whole genome shotgun sequence.
ACCESSION   DASSFQ010000235 DASSFQ010000000
VERSION     DASSFQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198861"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..>2047
                     /locus_tag="VFH96_08580"
     CDS             277..>2047
                     /locus_tag="VFH96_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6974064.1"
                     /translation="MMNRAGRRFYLFDNFRVDVNERLLFKDNRELALTPKVFDTLLVL
                     LENSSHVMTKKELMQQVWPDSFVEENNLAQNISILRKALGKTHEGEDYIQTVPKRGYR
                     FVGGVRVMGGEEESVIVHERTRARIVVERDDEDVARVIDVLPTAVSPKQLEPALQRPP
                     ETMYARSGDVNIAYQVIGDAPLDLVFVMGWVSHLEYFWREPSFARFLLRLASFSRLIL
                     FDKRGTGLSDRVPINQLPTLEQRMDDVRAVMDAVGSEHAALIGVSEGGPMCSLFAATY
                     PEKTLALVMIGTYAKRIRDAEYPWGPTLEQREHFFEVMREQWGGPVGIDERAPSVAED
                     PEFRDWWATYLRMGASPGAAVALTQMNAEIDVRNVLPSIRVPSLVIHRSDDQCLKVEE
                     GRFVAQRIPGAKFVELPGNDHLPFVGDQDAILDEVEEFLTGVRHPIEPDTVLATVLVA
                     RLVGIKERVQNLGPERWAEWWRRLHAHITKEIDWFRGREVDILENRLLAIFDGPARAI
                     RCANAITEYTSRLGVQTCTGLHTGECEIVDGQVAGAAAQMCVCVANEAEAGEVLVSST
                     VKDLVAGSGITFEDRGIHMLVGCG"
BASE COUNT          426 a          516 c          663 g          442 t
ORIGIN      
        1 cgttcaatta gcgtgacttc gtcaaagtga tcggataaaa ctcgcgcggc cagaagcccc
       61 gccaaacttc ccccaagcac cacggcgtgg cggccctggg cgtggccgtc gtaatcagcc
      121 tgacgcttcg aacttgtatt cattgcaact cctcctaaat aatctccgct cgagttgtcg
      181 ggtgtgagtg cgtggaagat acgggcagca ggcgagtctc gtatttagag aatcctgaaa
      241 tgcctttgag attgttttca aagagttctg aaagcgatga tgaatcgggc cggtagacgc
      301 ttctatctat ttgacaactt tcgagttgac gtaaacgagc ggctgctctt caaagacaat
      361 cgcgagttgg cgctcacgcc gaaagtcttc gacacgttgc tcgtgctgct cgaaaactcc
      421 agtcacgtga tgacgaagaa ggagttgatg cagcaggtgt ggcctgacag ttttgtcgaa
      481 gagaacaacc tcgcgcagaa catttccata ctgcgaaaag cgctcggcaa gacgcatgaa
      541 ggcgaagact atattcagac cgtgccgaag cgcggttatc gttttgtcgg cggcgtcaga
      601 gtgatgggcg gcgaagagga gagcgtgatc gtgcatgagc gcacgcgcgc gcgaatcgtc
      661 gtcgagcgtg atgatgaaga tgtcgctcgc gtgatcgatg tgttgccgac tgcagtttca
      721 cctaaacagc tcgaacccgc cttacaacga ccacccgaaa cgatgtacgc gcgcagcggc
      781 gacgtaaaca ttgcgtacca ggtaatcggc gacgcgccgc tcgatctcgt tttcgtgatg
      841 ggctgggtgt cgcacctcga atacttctgg cgcgagccga gctttgcgcg cttcctgctg
      901 cggctcgcgt cgttctcgcg actgatactt tttgacaaac gaggcacggg tctttccgat
      961 cgcgttccca tcaatcaact gccgacgctc gagcagcgta tggacgacgt gcgcgccgtg
     1021 atggatgcgg ttgggtcgga acacgcagcg ctgatcggcg tgtcggaagg tggtccaatg
     1081 tgcagtctct tcgcggcgac gtatccggag aagacgctgg cgctcgtgat gattggcacg
     1141 tacgccaagc ggattcgcga tgcagagtat ccgtggggac caacgctcga acaacgcgaa
     1201 cactttttcg aagtgatgcg cgagcagtgg ggtggaccag tcggcatcga cgagcgggcg
     1261 ccgagtgtcg cggaagatcc ggagtttcgc gactggtggg ccacgtattt gagaatgggc
     1321 gcgagtccgg gcgcggctgt cgcgttgacg cagatgaatg cagagatcga cgtgcggaac
     1381 gtgttgccgt cgatacgcgt gccgtcgctc gtgattcatc gttcagacga tcagtgtttg
     1441 aaagttgaag aaggacggtt tgtggcgcag cggataccgg gtgcgaagtt tgtcgagctt
     1501 ccggggaacg atcacttgcc gtttgtcggc gatcaggacg cgatactcga tgaagtggaa
     1561 gagtttctca ctggcgtacg acacccgatc gagcctgaca cggtgctcgc gacggtgctc
     1621 gtggcgcggc tggttggtat caaggagcgc gtgcaaaatc ttggtccaga gcgctgggcg
     1681 gaatggtggc gccgcttgca cgcgcatatc acgaaagaga tcgattggtt tcgcgggcgc
     1741 gaggtcgaca ttctggagaa tcgtttactg gcgatcttcg acggtccggc gcgtgcgatt
     1801 cgttgtgcaa atgcgatcac ggaatacaca tcgcgattgg gtgtgcagac ttgcaccggt
     1861 ctacatactg gcgagtgtga aatcgttgat gggcaggtag caggcgccgc ggcccagatg
     1921 tgtgtgtgcg tcgcaaacga agccgaggcc ggcgaggtgc tggtgtcgag cactgtgaaa
     1981 gatttggtgg caggctcggg aatcactttc gaagaccgtg gcattcacat gctcgtgggt
     2041 tgcggcg
//
LOCUS       DASSFQ010000236         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2198942, whole genome shotgun sequence.
ACCESSION   DASSFQ010000236 DASSFQ010000000
VERSION     DASSFQ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198942"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="VFH96_08585"
     CDS             complement(<1..1595)
                     /locus_tag="VFH96_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HET6974065.1"
                     /translation="MSLRVSTFIDLLQYRALKTPDRTAFIFLEDGETETARITYGELH
                     QRARAIGAWLQLHKAAADRVLLLYPSGLDYIAGLFGCMYAGAIAVPAYPPRHNRTLDR
                     LEGIINDAQPAFALTTQDIHERVLDGNSENLRGIKIVEADRIDKTLTEFWVPPLLSER
                     ALAFLQYTSGSTTTPKGVMLTHANLLFNERLIQKIFQQTEKSTVAGWLPLYHDMGLIG
                     SVLQPIYNGSSCVLMPPAAFLQQPIRWLQTISRYRATTSGGPNFAYELCVRKIDEEQK
                     AALDLSTWSVAFNGAEPIRPESIEHFTKAFGPCGFSPKAFRPCYGLAEATLIVSGQKK
                     PAPPLVKRVEADALTYDRVVQATDETDQARTLIGCGHALGQELIIVNPETLTRCSASR
                     VGEIWLSGRGVAQGYWNNQGETKAVFRAHLANGDGPYLRTGDLGFKHKNELFITGRLK
                     DLIIIRGVSHYPQDIEHTVAHAHENLMNHRGAAFSMEIDGEERLAVIHEIRPRRTTDF
                     DELVNAIRHAVAREHGVNVYTVG"
     gene            complement(1623..2639)
                     /gene="sbnA"
                     /locus_tag="VFH96_08590"
     CDS             complement(1623..2639)
                     /gene="sbnA"
                     /locus_tag="VFH96_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HET6974066.1"
                     /translation="MRSQPESDWSKCLTEGVLAAVGNSPLVQLKRIFRDAHFHLFAKL
                     EATNPGGSSKDRAALSIISKAIEKGRIRPGTTIVESSSGNMGIGLAQICAYLGLRFIC
                     VVDPKTTTLNLDLLKAYGASVDMVTECARPGDFLQARIDRVQALLRLIPDSYWPNQYA
                     NLDNAKAYYETMREIGNSLGRDPDYLFCATSTCGTVRGCAEYVRTHELKTKVIAVDAV
                     GSVIFGGKVRKRLIPGHGAARRPELYAPKLAEECVHVSDLDCVVGCRRLVLHEAIMAG
                     GSSGGVITAVERFKHEIPKDATCVVILPDRGERYLDTVYSDAWVKEHFGDVSHLWEIA
                     RAAA"
     gene            complement(2734..>3267)
                     /locus_tag="VFH96_08595"
     CDS             complement(2734..>3267)
                     /locus_tag="VFH96_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974067.1"
                     /translation="LDQPCCRQFADRCEAEFGFTGIEFAADANELLQNCSLVSLATTA
                     SQPHLSSLPETTLPQTILHISLRDLSPEIILSADNVVDDADHVCRARTSLDLAAQVAG
                     NRDFIRCSLADVLTGKQTPRDGVHNTTIFSPFGLGILDLAVSKLVRDLAQASASGTVV
                     PSFLPDSWRDQVSSNHG"
BASE COUNT          704 a          931 c          929 g          703 t
ORIGIN      
        1 aaaccgacgg tgtaaacatt gacgccgtgt tcgcgcgcga ccgcgtgacg aatcgcgttg
       61 accagctcgt cgaaatccgt tgtgcgacgc ggccgaatct catgaatcac cgcgagccgt
      121 tcctcaccgt caatctccat cgagaacgca gcgccgcgat gattcatcaa attctcgtgt
      181 gcgtgcgcga ccgtgtgttc gatatcctgc ggataatgac tcacgccgcg aatgatgatc
      241 agatctttta atcgaccggt aatgaagagc tcgttcttgt gcttgaaacc aagatcgccg
      301 gtgcgcaggt aaggaccatc accgttcgcg agatgagcgc gaaagacggc cttcgtttcg
      361 ccttgattgt tccagtaacc ttgcgccact ccccggcccg acaaccagat ctcaccaacc
      421 ctgctcgccg agcatctcgt cagcgtctca gggttgacga tgatcagttc ctgtccgagt
      481 gcgtgaccgc aaccgatcaa cgtgcgagcc tgatcggttt catcagtggc ctgcaccaca
      541 cggtcgtaag tcaaagcgtc cgcttcaact cgtttcacca gtggaggcgc gggtttcttt
      601 tggccggaaa cgatcagcgt cgcttcggca agtccataac agggccggaa agctttcgga
      661 gaaaagccac acggaccaaa tgccttcgtg aaatgctcga ttgactccgg gcgaatcggt
      721 tcggcgccgt tgaatgcaac gctccacgtg cttagatcga gcgcagcttt ctgttcctcg
      781 tcgatcttgc ggacgcaaag ttcgtaagcg aagttgggac caccactcgt cgtcgcccga
      841 taacgcgaaa tcgtttgtaa ccaccgaatt ggctgttgca ggaatgccgc aggcggcatc
      901 agcacgcaac ttgagccgtt gtagattggt tgcaacacgc tgccaatcag acccatgtcg
      961 tgatacagcg gcagccagcc cgcgacggtc gatttttcag tctgctggaa gatcttctga
     1021 atcaggcgtt cgttgaagag caggttggcg tgagtcaaca tcacgccttt cggcgtggtg
     1081 gtcgacccgg aggtgtattg caggaacgcc agcgcgcgtt cgctgagtaa tggagggacc
     1141 caaaactccg tgagagtctt gtcgatccga tcggcctcga cgattttgat gccacgcaaa
     1201 ttttctgaat tgccgtcgag aactctctcg tgaatgtctt gagtggtgag tgcaaacgcg
     1261 ggctgcgcgt cgttgatgat gccttcgaga cgatcgaggg tgcgattgtg acgcggtgga
     1321 taagccggca cggcaatcgc gccggcgtac atgcaaccga acagaccggc gatgtaatcg
     1381 agaccggaag gatagagcag cagcacgcgg tcggcggccg ctttgtgcaa ctgcaaccag
     1441 gcgccaatcg cacgcgcacg ctgatgcagc tcgccgtaag tgatgcgagc cgtctccgtt
     1501 tcgccgtctt caagaaagat gaacgccgtg cgatcaggcg tcttcaacgc gcgatactgg
     1561 agcagatcta tgaatgtcga aactcttaga gacacggatc agacgttgat ttcttttgga
     1621 cttcacgcgg cggcgcgggc gatttcccag aggtgtgaga cgtctccaaa gtgttctttg
     1681 acccacgcgt ctgaatagac agtgtcgagg taacgctcgc cgcgatcggg caggatcaca
     1741 acgcacgtgg cgtctttcgg aatctcgtgc ttgaaacgtt cgaccgcggt gatgactcct
     1801 cccgacgaac cgccggccat gatggcttcg tgcaacacca gccgccggca gccaaccacg
     1861 caatcgagat ccgaaacgtg cacgcactct tctgccagct tcggcgcgta aagttcgggc
     1921 cgtcgcgcag caccgtgacc gggaatcagc cgcttgcgca ccttgccgcc gaagatcacg
     1981 ctgccaaccg catcgacagc gatcaccttc gtcttcaact catgcgtgcg aacgtactcc
     2041 gcacaacccc gcactgtgcc gcacgtgctc gtcgcgcaga agagatagtc aggatcgcgc
     2101 ccgaggctgt tgccgatctc gcgcatcgtc tcgtaataag ctttcgcgtt gtcaaggttc
     2161 gcatactggt tgggccagta actatcggga atcaagcgca gcaacgcctg cacacgatcg
     2221 atgcgcgcct gcagaaagtc tcccggccgc gcgcactccg ttaccatgtc gaccgacgcg
     2281 ccgtacgcct tcaacagatc gagattcagt gtcgtggtct tgggatcgac gacgcagatg
     2341 aaacgcaagc caaggtaagc gcaaatctgg gcgagcccga tgcccatgtt gccggagctc
     2401 gattcgacga tcgtcgttcc cggcctaatc ctgcccttct cgattgcttt gctaatgatc
     2461 gacaacgcag ctcgatcttt tgagctgcca ccgggattgg ttgcttcaag tttcgcaaag
     2521 agatgaaagt gagcgtcgcg gaagattcgc ttcagttgga ccagcgggct gttgccaact
     2581 gcggcgagca cgccttcagt cagacactta ctccaatcgc tctcgggctg agatcgcatt
     2641 ttattagtcc tgctttttga gtgtttattg aattacccag gccgttcttt taaccacaga
     2701 taaaatggat agaaatccgg atttttctcc gtgttatccg tggttactac tgacctgatc
     2761 tctccacgag tcggggagga atgagggcac gacggtgccg ctggcgctgg cctgagccag
     2821 gtcgcggacc agtttgctca cggccagatc gagaatgccg aggccgaagg gactaaagat
     2881 cgttgtgttg tggacgccgt ctctcggggt ttgtttaccg gttaagacgt cggcgagtga
     2941 acagcgaatg aagtcgcgat tgccggccac ttgcgccgcg aggtcgagcg acgtgcgcgc
     3001 gcgacaaacg tgatccgcat catccacgac attgtcggcg ctgagaatga tctccggcga
     3061 cagatcgcgt aaagagatgt gcagtattgt ctgaggcaaa gttgtctcgg gaagactaga
     3121 gagatgcggc tgcgatgcag tcgtggccag cgatacgagc gaacagtttt gcaaaagctc
     3181 attcgcatcc gcagcaaact cgattccagt aaacccgaac tccgcttcac atctgtccgc
     3241 aaactgccgg cagcatggtt ggtccag
//
LOCUS       DASSFQ010000237        15135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1142939, whole genome shotgun sequence.
ACCESSION   DASSFQ010000237 DASSFQ010000000
VERSION     DASSFQ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15135
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142939"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFH96_08600"
     CDS             complement(<1..1147)
                     /locus_tag="VFH96_08600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6974068.1"
                     /translation="MTSTRKALIAAAVAVIFATGLIFWQVKARKSGPVELTPEDMALI
                     AEDQPPQMRARLATDETARKDFALQVRQLLAVAEEAEANGVGNAPEMKRQLEFQKASV
                     IAQKYLEKQGEGGSGDITDAEVDEFYKQPGNQAKFDQIIADAKSENPQLASQEIPPEQ
                     LNMLKQRLGRIYIAEKKAIDKGMDKEPGVRLQMLLQHARVIAQKYAVDKLQDKMKATD
                     EEVNAYLTSHPELDTDKKNRAKAEEVLKRARAGEDFGKLAAEFSTDGSKDKGGDLGWF
                     GRGQMVPEFEQAAYALKPGEISDVVQSKFGYHIIKLEERKNETKDGKTEEKVHARHIL
                     ISDAAANPFGPPQSSRDRAKAAVESDKAKKVLDEIVSRSHVKVPDNYT"
     gene            complement(1166..1645)
                     /gene="mce"
                     /locus_tag="VFH96_08605"
     CDS             complement(1166..1645)
                     /gene="mce"
                     /locus_tag="VFH96_08605"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET6974069.1"
                     /translation="MLCLTGWPTKLRRAKETRILRWRRLFRLLKVDHIGIATKSIEEG
                     LEMWRDVLGLRVDSTEEVAEQGVKACMLAVGDTHVELLEPLAPDTPVGRFLAKRGPGM
                     HHIAIEVADIHASLAELKKKGARLIDETPRVGVGGCLVAFVHPSSVNGVLLELVQHQ"
     gene            complement(1551..2498)
                     /gene="meaB"
                     /locus_tag="VFH96_08610"
     CDS             complement(1551..2498)
                     /gene="meaB"
                     /locus_tag="VFH96_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET6974070.1"
                     /translation="MVTNNLSERVNRGEAAAVARAISKIEDGADGAAELMKEIYQRSR
                     GALVIGITGAPGAGKSSLVDKLAMFYRKNGERVGIVAVDPSSPFSGGAILGDRIRMQA
                     LGLDDGVFIRSMATRGNLGGLARATVDAVAILDAAGYAKIIVETVGVGQDEVEVVKAA
                     DVSVVVLVPGMGDDIQAIKAGIMEIGDIFVINKADKEGVYATEKELESLLSLASRDDG
                     WDPPIVKTVATENHGVADLAQAIERFSTAQLGPRVSSDRRRAIARWRIVELLRESLLA
                     RVLGTDASVAMLNRLADEVATRQRDPYSAVEEIIQAAKS"
     gene            complement(2531..3676)
                     /locus_tag="VFH96_08615"
     CDS             complement(2531..3676)
                     /locus_tag="VFH96_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6974071.1"
                     /translation="MDFELTEEQQQIKMSVREFAESEIAPHVMEWDESQHFPIELKPK
                     LAELGLMGVLFPEEYGGAGMGYVEYATIIEELSRVDGSVGISVAAHNSLCSNHIYQYG
                     TEEQKKKYLAPLASGEHLGAWGLTEPSAGSDASGTKSTAVPADGGWIVNGSKNFITHA
                     IHADTCVAMAVTDRAQRSKGISAFIFEKGQKGFSPSKKENKLGLRASETASVVFEDCF
                     VPQDQMLGEEGQGFVNSMQILDGGRISIAALAVGIAQGAYESAVRYAKERQQFGKPIA
                     EFQGIQFKLADMATQIDAARLLMYRAAWMKDAGKKTTKESSMAKLYASEVSVKVCEEA
                     IQVHGGYGYTKDYPPEKYWRDSKLCTIGEGTSEIQRIIIARELLRTI"
     gene            complement(3705..4481)
                     /locus_tag="VFH96_08620"
     CDS             complement(3705..4481)
                     /locus_tag="VFH96_08620"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6974072.1"
                     /translation="MVDRKLGSMEQRLQKLIAAAGVASRRHAEELIAAGRVTVNGEVI
                     KELGTKADPQKDHIKVNGKLINPQLQSREKVYVLLNKPKGYLSSVSDPEGRPLVTELL
                     PPALGKVHPVGRLDFNTEGLLLLTNDGDFTNYVTAARNRVEKVYEVKVKGVPAESGIE
                     RLRRGITLEDGTRTAPAKIVKLHETQTNAWYEVLLHQGRNQQVRKMFELIGHSVLKLR
                     RTRIGFLRDENLKPGYWRLLSPGEVGRIMKKKKAGHGLRG"
     gene            complement(4468..5160)
                     /gene="scpB"
                     /locus_tag="VFH96_08625"
     CDS             complement(4468..5160)
                     /gene="scpB"
                     /locus_tag="VFH96_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET6974073.1"
                     /translation="MASNEETVNQEPVDEAALNEEASRELAAMVAEAEQTLGATEAEG
                     DDDGPLSAPQSGAERAGVIEALIYVSDEPLSVKTIADVLKEDKQVIVEAVAALAQEFN
                     ARNGGLQMREVAGGWQFATRPEYHEHVRSFLKTRPSAKLSIASLETLAVIAYRQPVTV
                     PEILEIRGVQSPSAIKTLLDKKLIVAKGRKETVGRPMMYGTSKEFLMQFGLKDLSELP
                     SVEDFHDLSGGS"
     gene            complement(5188..5982)
                     /locus_tag="VFH96_08630"
     CDS             complement(5188..5982)
                     /locus_tag="VFH96_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET6974074.1"
                     /translation="MNDNIPEAVDTAEERTPLNVAGAVPHIVSDDTTDDLKILLGEFE
                     GPLDLLLHLIRQEHVSIYDIPVARITDEYLRYLRVMQEMDIAVAGDFLVMAATLIELK
                     TKMLLPSDPTAPPEEEEDPRSDLVNQLLEYQKYKAAAQMLWSRATVEQAVFKRAEIET
                     DKNNPEVAVGLFDLLKVFQDILARHKEEKLLEIEREEISMAEMLERLRNMVMSSGELN
                     LRVFFERARSRRELVLAFLSVLELVRTTEVKLFQRETFGDIIARSA"
     gene            complement(5993..6976)
                     /gene="trpS"
                     /locus_tag="VFH96_08635"
     CDS             complement(5993..6976)
                     /gene="trpS"
                     /locus_tag="VFH96_08635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET6974075.1"
                     /translation="MPKRIFSGAQPTGNVHLGNYLGALRNWVALQHEYESFFCIVNLH
                     AITLPQNPALLAEKTRDLARIYLAVGIDPQVSTVFIQSHVKEHAELTWVLNCVARMSE
                     LERMTQYKDKARKQQENISVGLFDYPVLMAADILLYHTDLVPVGDDQRQHLELTRDVA
                     IRFNRDYGETFKVPDAYIPKVGARIMSLSDPTKKMSKSDDDPNGCVLLMDDADTVQKK
                     FKRAVTDSGTEIRFDASRPAINNLLEIYHLITGKPVPAVEEHFAGKGYAQLKGDLAEA
                     TIEFLRPFQERVRAISDEELDAILDQGREKAQQIASATLKEVFARTGLGHR"
     gene            complement(7003..7680)
                     /locus_tag="VFH96_08640"
     CDS             complement(7003..7680)
                     /locus_tag="VFH96_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6974076.1"
                     /translation="MSPEQLIPQLVIYMVVLLLAISAHEAAHAWMSYKFGDDTARLLG
                     RITLNPVAHTDPIGTLLIPIAGFIFGAMGGGRFPLIGWGKPTPVNPLRWRNKDVANVM
                     VSIAGILANLLIAVIAFIIFKFLLMSGLILAMSDSTQEPVILFLDNLLIMNISLAVFN
                     LLPFPPLDGSKVLETFLPASMQPALEFLERYGYFLLILLIYMGVFTAIIRPIINFVHY
                     LLLLGVR"
     gene            complement(7711..8736)
                     /locus_tag="VFH96_08645"
     CDS             complement(7711..8736)
                     /locus_tag="VFH96_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET6974077.1"
                     /translation="MGSAGKIIAVDPPYAIAGGEIVIDCTGLDTREPSKCGVLIGDTP
                     AQVVALGPRRVLVIVPETKGGRVPVTLWNGDEASEPAYLTVARKLAGDLHPVSNPAFD
                     PDDGALFVTRSGSRGEELPVTLFRIDISGDVSEYSGDITNPTGIAFSPEGQMFVTSRL
                     EGTVYKITPFKEAVSFAHNLGVATGIAFNSDGLMHIGDRTGTIFKVNGIGEERAWTQL
                     EPSVSAFHLAFGPDDSLYVAGPTVTSFDAIWRINPDGDIDMFYKGLGRPQGLAFDRDG
                     NLYVAAALRGRRGIVRIAPDGVDAKLVVAGVNLIGLAFSATGEMAVVSIDSVYSLPTQ
                     IKGTLLR"
     gene            8981..10534
                     /gene="hflX"
                     /locus_tag="VFH96_08650"
     CDS             8981..10534
                     /gene="hflX"
                     /locus_tag="VFH96_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET6974078.1"
                     /translation="MSHETRRQIGALIDRQGHIQYVMVGDNRRIELPDFKRVRVASGR
                     FRGLRCVHTHLRGEPLTQDDLTDLALLRLDLMAAIDVDAESGLPGLVRAAHLLPLHAG
                     DLDNATTPELYAFLEPKIPSQLDHDFRELINSLEEEMARNRRTTRRAEVRDRTILVGV
                     ATGSLADAEESMAELAELATSAGVVVQDRIIQRRQAIDPRTVLGRGKLDELIIRALQL
                     GADLLVFDSELSPAQVRSLSEATDLKVIDRSQLILDIFAQRAQSKEGKIQVELAQLKY
                     LLPRLIAGQDSAFSRLAGGIGGRGPGETKLETDRRRVRDRINRLEREIEALQQRRQER
                     RKGRTRNGLPIISIVGYTNAGKSTLLNALTKSDVHAEQRMFATLDPTSRRLRLPREQE
                     VIINDTVGFIRALPPDLLSAFRATLEEISDSTLLIHAVDISNPRWVQQVQSVKRILAD
                     LKLAEIPSILALNKADLVDAETVEAAVRQARQLDSGETVVISAIKPSTLDALLEKAGA
                     VLARNLAAA"
     gene            10647..13037
                     /locus_tag="VFH96_08655"
     CDS             10647..13037
                     /locus_tag="VFH96_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET6974079.1"
                     /translation="MGLRPRFKTPVSQARDKLIAYAMRPLMVFKPRTRFLIACSALVL
                     LTTLLLLTNRGSSFNENYKLGEVIGRSVIAPTDLTAVDQAETERRKNIARETSRPVFN
                     YDSSRAETSVQSFRSDWDDLKTQATEGQSKSLVWSGEGGAAVAQAIAAHKFNEADLER
                     IASILRDSSAGYIYDDAEADRLQREIVLIDVRNSSSPVIMPAPRTSMKALSTARRTLE
                     QRIVNLPAWSAVQKNALNSALAPLIRPNVVLDPSATATARETEASSVEPVVISLKRNQ
                     VVAREGDTVTPGILAQINAIKSSGNSGRPWNNLIGLLLVVIAVYWVAWKFAEHRSGSS
                     LSLSKHKAFALVGSAILVQTALMKVGFAFGDSMAARMQAPFNDPVIWNFAIPFAAAAL
                     LVAMLVDTQLAFLTGIITALFAGMLAPTGMQKALFAMVSCSSAIYGIGRYRERQSVTL
                     AGLLVGGVNAVMAIALIAYSEQPLTLRTILMAAAAGITGGLLTAVFTAGGLPINESLF
                     GILTDVKLLELSNADLPVLGQLAMRAPGTNQHSHAVGQLAEDACRAVGANPLLARIGA
                     LYHDIGKLAAPEYFVENQQGDNPHDRLRPYQSAKIITSHVTYGARLAKEIGLPQKIAD
                     FIPQHHGTRTLHYFLRKAQAQAKPGENVDEGDFRYPGPKPQFKEAAIMMLADSCEAAA
                     RSLARPDPENIRAIVLKIFDSIISDGQLDQCDVTLQELTIIREAMISALTAIYHARID
                     YPGFNPPAMTGPLPPLPAGNLDSEERGITYSKPSEVPISRGGEVEDEAVPRRVSKR"
     gene            complement(13097..13591)
                     /locus_tag="VFH96_08660"
     CDS             complement(13097..13591)
                     /locus_tag="VFH96_08660"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET6974080.1"
                     /translation="MERLWSPWRARYIASAIDAQNDVCVFCAIASDPEHDEENYVLHR
                     APHCFVLLNLYPYISGHLMIVPYLHTSELDSMAKEITDDMMDQTKRAQAALREVYKPA
                     GFNLGMNLGLAAGAGIADHLHIHLLPRWVGDTNFMTTVGETRVLPEDLPTAYSKLRPK
                     FATD"
     gene            complement(13702..13968)
                     /locus_tag="VFH96_08665"
                     /pseudo
     CDS             complement(13702..13968)
                     /locus_tag="VFH96_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(14138..>15135)
                     /locus_tag="VFH96_08670"
     CDS             complement(14138..>15135)
                     /locus_tag="VFH96_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET6974081.1"
                     /translation="NPNELFKVGDTIQVKVLKFDRDRERVSLGYKQLLPDPWSSVEER
                     FPVGTRVSGRVASVADYGAFVELEPGVEGLVHVSEMSWSKRVKHPSKVVNPGDLVEVE
                     VLSVDPRARRISLGMKQVQENPWQTLHERYQVGTRVHGRVRNLTDFGAFIEIEDGVDG
                     LVHVSDISWSRRIKHPSEVLKKGQEIDAVITSIDTENRRLSLSIKDLEPNAWEKFTNE
                     HKPGDVVHGKVARFANFGAFIELDDNLEGLCHISELSEERVAKPEDVVQLGQEMDFKI
                     LRIDPETKKIGLSARAVGKDEPIIDTKIYSSEAGGGMASLGELADFGLSKPDNDQ"
BASE COUNT         3227 a         4632 c         3959 g         3317 t
ORIGIN      
        1 ccgtgtagtt gtcaggcact ttcacgtgcg aacggctcac gatctcgtcg agcactttct
       61 tcgccttgtc tgactcgact gcagccttgg cccgatcgcg tgagctttgc ggaggtccaa
      121 atggattggc cgctgcgtcg ctgatcagaa tgtggcgcgc atgcaccttc tcttctgttt
      181 tgccgtcttt ggtttcgttc tttctctctt cgagcttgat gatgtggtaa ccaaacttgc
      241 tctggaccac gtcgctgatc tcgcccggct tcagtgcgta agcggcctgc tcaaactcgg
      301 gaaccatctg cccacgtccg aaccaaccga gatcgccacc cttgtccttg ctgccgtcag
      361 ttgaaaactc cgccgcgagt ttgccgaaat cttcaccagc gcgcgctcgc ttcaaaacct
      421 cttccgcctt ggcgcggttc ttcttgtccg tgtcgagctc aggatggctg gtgaggtacg
      481 cgtttacttc ctcatcagtc gccttcatct tgtcctgcaa cttgtccacc gcgtatttct
      541 gcgcaatcac ccgcgcgtgc tgcagcagca tttgcaaacg aacacccggt tccttgtcca
      601 tccccttatc aatcgccttc ttctcagcga tgtagatccg gccgaggcgc tgcttgagca
      661 tgtttagctg ctcgggtgga atctcctgtg acgccaactg cggattctcg ctcttcgcat
      721 cggcaatgat ctgatcaaac ttcgcctgat tgcctggctg tttatagaac tcgtcgactt
      781 cggcgtcagt aatgtcgccc gacccgccct caccctgctt ttcgagatac ttctgagcaa
      841 tgacgctggc tttctgaaac tcgagctgac gcttcatctc gggcgcgttg cccacgccgt
      901 tggcttctgc ctcttcagca accgcgagca gttgtcgcac ctgcagcgcg aagtccttgc
      961 gtgctgtctc gtcagtggcc aggcgggcgc gcatttgcgg cggttgatct tcggcgatga
     1021 gcgccatatc ttcaggtgtg agttcgaccg ggcctgattt tcgggcttta acctgccaga
     1081 agatcaaacc cgtcgcaaag atcactgcca cagcggctgc gatgagagct tttcttgttg
     1141 aagtcaaaat actgtccttt cctaactatt gatgctgcac cagctcgagc agcacgccat
     1201 ttaccgatga gggatgcaca aaagcaacga gacaaccgcc gacacccaca cgcggcgttt
     1261 cgtcgatgag tcgtgcgcct tttttcttca attcagcgag cgaggcgtgg atatccgcaa
     1321 cctcgattgc aatatggtgc attcctggcc cacgtttagc gagaaacctt ccgacgggcg
     1381 tgtcaggagc aagcggttcg agcagctcga cgtgagtgtc acccacggcc agcatgcatg
     1441 cctttacgcc ttgttcggcg acttcctcgg tcgagtcgac tctcagcccc agcacatcgc
     1501 gccacatttc caaaccctct tcgatgctct tagtggctat tccgatgtga tcaactttta
     1561 gcagcctgaa taatctcctc caccgcagaa tacgggtctc tttggcgcgt cgcaacttcg
     1621 tcggccagcc tgttaagcat agcaaccgag gcgtcagttc ccaaaacgcg cgcgagcaga
     1681 ctttccctca ataactcaac aattcgccag cgcgcgatgg cgcggcgccg gtcgctgctc
     1741 acgcgaggcc cgagttgtgc ggtagaaaaa cgctcaattg cctgcgccag atcggccacg
     1801 ccgtgatttt cagtggcgac agttttgacg attggcgggt cccagccatc gtcgcgggaa
     1861 gccagcgaca ggagcgattc gagctccttc tcggtggcgt aaacaccttc tttatcggcc
     1921 ttattaataa cgaagatgtc tccgatttcc atgattccgg ccttgattgc ctgaatatcg
     1981 tcgcccatgc ctgggactaa cacgaccacg gaaacatcag cggctttgac gacttcgact
     2041 tcgtcctgac cgacgccgac cgtctcgacg atgatcttcg cataaccagc agcatcgaga
     2101 atcgcgactg cgtcgactgt cgcgcgcgcg aggccgccga gattgccacg cgtggccatc
     2161 gagcggatga aaacgccgtc gtccaatccg agcgcctgca ttcgaatgcg atcgccgagg
     2221 attgccccgc cggaaaacgg gctcgacgga tcgaccgcaa cgataccgac gcgctcgccg
     2281 ttcttgcgat aaaacatcgc gagcttgtct acgagtgatg acttgccggc gccgggcgcg
     2341 cccgtgattc caatcacaag ggcgccgcgc gagcgttgat aaatctcctt catcaactcg
     2401 gcagcaccat ccgcaccatc ttcaatcttc gagatggcac gcgccaccgc cgccgcctca
     2461 ccgcgattaa cgcgctcact gaggttattc gtaaccattc tcttaatagg gcctatagga
     2521 ccaatagaac ctatattgtt ctcagcagct cgcgcgcgat gatgatgcgc tggatctccg
     2581 aagttccttc tccaatcgtg cagagcttcg agtcgcgcca gtatttctca ggcggatagt
     2641 ccttcgtata accatacccg ccgtgtacct ggatcgcttc ctcgcaaact ttcacactca
     2701 cttcagacgc atacagcttc gccatcgacg actctttcgt cgtcttcttc cctgcatcct
     2761 tcatccacgc cgcgcgatac atcaacaagc gcgctgcatc gatctgcgtc gccatgtccg
     2821 caagcttgaa ctgaatcccc tgaaactccg cgatcggttt tccaaactgc tgccgctctt
     2881 tcgcataacg cactgccgac tcgtaggcgc cctgcgcgat accgaccgcc aacgccgcga
     2941 tcgagatacg accgccgtcg agtatctgca tcgaattcac aaagccctga ccttcttcgc
     3001 ccagcatctg atcttgcggc acgaagcagt cttcaaaaac gacgctcgcg gtttctgacg
     3061 cgcgcaatcc gagcttgttc tctttcttcg acggcgagaa acctttctgg cctttctcga
     3121 aaatgaacgc cgagatgcct ttgcttctct gcgcccggtc agtcactgcc atcgccacgc
     3181 aagtgtcagc gtggatcgcg tgcgtgatga agtttttcga gccgttgacg atccagccac
     3241 catcggcggg cactgcggtc gacttcgttc ccgacgcgtc cgagccggcg ctcggctcag
     3301 tcagtcccca cgctccgaga tgctcgccac tcgctaacgg cgcgagatac tttttcttct
     3361 gctcttccgt accgtactgg taaatgtgat tcgagcagag cgaattgtgc gcggctacgg
     3421 agatgccaac cgagccatcg acgcgcgaga gctcttcgat gatcgtcgcg tactcgacat
     3481 accccattcc ggcgccgcca tactcttccg gaaaaagcac acccatcaaa ccgagctcgg
     3541 cgagtttcgg tttgagctcg atgggaaagt gctgcgattc atcccactcc atgacgtgag
     3601 gcgcgatctc tgattcagcg aactcgcgca cgctcatctt gatctgttgt tgctcttcag
     3661 ttaactcaaa atccatagtg cttttcttgt aacataaact gtgtttatcc gcgtaatccg
     3721 tggccagcct ttttcttctt cattattcgc ccgacttctc ccggcgataa gagccgccag
     3781 taaccgggct ttagattctc atctctcaaa aacccgatgc gcgtgcgccg cagcttcaac
     3841 actgagtgcc caatcagctc aaacatcttt cgcacttgct ggttgcgtcc ctggtgcagc
     3901 aacacttcgt accaggcgtt ggtttgagtc tcgtgcagct tcacgatctt tgcaggcgct
     3961 gtgcgcgtgc cgtcttcgag cgtgatgccg cgccgcaatc gttcgatgcc actctccgcc
     4021 ggcacaccct tgactttgac ttcgtaaact ttctcgacgc ggttacgcgc cgcggttacg
     4081 tagttggtga agtcgccatc gttggtgagt aacagcagac cttcggtgtt gaagtcgaga
     4141 cgcccgaccg gatggacctt accaagcgcc ggcggcaata gctcagtaac gagcggccgc
     4201 ccctcgggat cggaaacact cgagagataa cccttcggct tgttaagaag tacgtagact
     4261 ttttcgcgag actgtagctg aggattgatg agcttgccgt tgaccttgat gtgatctttt
     4321 tgcggatcgg ccttcgtgcc gagctctttg attacttcac cattaaccgt aacccgaccc
     4381 gcagcaatca gctcttccgc atgcctgcgt gaggcaactc cagccgcggc aatcaatttt
     4441 tgcagtcgct gctccataga tccgagttta cgatccacca ctcagatcat ggaaatcctc
     4501 gacgcttggc aactccgaca gatccttcaa cccaaactgc atcaaaaact ctttcgacgt
     4561 cccgtacatc atcggtcgcc cgacagtttc cttgcgtccc ttcgcgacga tcagcttctt
     4621 atcgagcaac gtcttgatag ctgatggcga ctgcacgcca cggatctcga gaatctccgg
     4681 aacagttacc ggctgtcgat aggcaatcac agcgagcgtc tcgagtgatg cgatgctgag
     4741 tttcgcactc ggccgcgtct tcagaaacga gcgcacgtgt tcgtgatact caggccgcgt
     4801 cgcaaactgc caaccaccgg cgacttcacg catctgcaaa ccaccgttgc gcgcattgaa
     4861 ctcctgcgcc agtgccgcca ccgcttcaac gatcacttgt ttgtcttcct tgagaacgtc
     4921 agcgatcgtc ttcacactca gcggctcatc cgaaacatag atcagcgcct cgatcacacc
     4981 agcgcgctca gcaccgctct gcggcgccga caaaggccca tcgtcgtcgc cctctgcttc
     5041 agtagcgccc aacgtctgct cagcctcggc caccatcgcc gccagctcgc ggctcgcctc
     5101 ttcattcagc gccgcttcat cgactggctc ctggtttact gtttcctcgt tgctcgccat
     5161 gttttgttga gtctacccgg gtaactgtta cgctgaacgc gcgatgatgt cgccaaaagt
     5221 ctcgcgctga aatagtttga cctcagtcgt tctcactagc tccaacactg acagaaacgc
     5281 cagcaccagc tcgcgtcgcg atcgcgcgcg ctcaaaaaac acacgcaggt tgagctcgcc
     5341 cgacgacatc accatgttac gcaaacgttc gagcatctca gccatcgaga tctcttcacg
     5401 ctcgatctcg agtaacttct cttccttgtg tcgcgcgaga atatcctgaa aaaccttcaa
     5461 cagatcaaac aggccaaccg cgacttccgg attgtttttg tcagtctcaa tttccgcacg
     5521 tttgaataca gcctgctcga cagtcgcgcg ggaccagagc atctgcgcgg cggctttgta
     5581 cttctgatac tcgagcaact ggttcaccag atcactacgc ggatcttctt cttcctcggg
     5641 cggcgcagtg ggatcgctcg gcagcagcat cttcgtcttc agctcgatca gcgtcgccgc
     5701 catcaccaga aaatcacccg cgaccgcgat gtccatctcc tgcatgacgc gcaggtaacg
     5761 aagatactcg tccgtaatgc gcgcgaccgg aatgtcgtaa atgctgacgt gctcctgacg
     5821 gatgaggtga agcaacagat cgagtggacc ttcaaactcg ccgagcaata tttttaagtc
     5881 atcggtagtg tcgtcggaga cgatatgcgg caccgcgccg gccacgttca gtggggtacg
     5941 ctcctcggca gtatcaacgg cttcaggaat gttatcgttc atcaaatccg tgttatctgt
     6001 ggcctaagcc cgtgcgcgcg aatacctctt tcagagtcgc gctcgcaatc tgctgcgcct
     6061 tttcacgtcc ctggtccagg atcgcatcga gctcctcgtc actgatcgcc ctgacacgtt
     6121 cctggaacgg ccgtagaaac tcgatcgtcg cttccgcgag atcgcctttt aactgagcat
     6181 aacctttacc cgcgaagtgc tcttcaactg ccggcacagg cttgccggtg atcaagtgat
     6241 agatctcgag caggttgttg atcgccggcc gcgacgcgtc aaaccggatc tcagttcccg
     6301 agtcagtcac cgcgcgcttg aacttctttt ggaccgtgtc agcatcgtcc atcaacaaaa
     6361 cacaaccatt cggatcgtcg tctgacttcg acatcttctt cgtcggatca gataacgaca
     6421 tgatgcgcgc gccgactttc ggaatgtaag catccggcac tttgaacgtc tcgccgtaat
     6481 cgcgattgaa tctgatggcg acgtcacgcg tcagctcgag atgttgtcgc tgatcgtcac
     6541 caaccggcac gaggtccgtg tgataaagca atatatcggc agccatcaac accggatagt
     6601 cgaacagacc gaccgagatg ttttcctgct gcttgcgtgc cttgtctttg tactgcgtca
     6661 tgcgctcgag ctctgacatg cgcgcgacgc aattcaatac ccacgtcagc tctgcgtgct
     6721 ctttaacatg cgactgaatg aagactgtcg acacttgtgg atcgattccg acggccagat
     6781 agatccgggc gagatctctg gttttctctg cgagcagcgc cgggttttgc ggcagagtga
     6841 tcgcgtgcag gttcacgata caaaagaagc tttcgtattc gtgctgcaag gcaacccagt
     6901 tgcgcaacgc gcccagatag tttccgagat ggacgttgcc tgttggttgc gcgccactaa
     6961 agattcgttt cggcatgagt taaaactatg agtgcgattg gctcagcgta ctccaagcag
     7021 gagaaggtaa tgaacgaaat tgatgatcgg tcttatgatt gctgtaaaga cccccatgta
     7081 aatcaaaaga atcaaaagaa agtagccata gcgttcgagg aattccagcg ccggctgcat
     7141 gctggccggt aaaaacgttt cgagcacttt gcttccatca agcggcggaa acggcagcag
     7201 attgaagacc gccagcgaga tgttcatgat cagcaggttg tcgagaaaca gtatcaccgg
     7261 ctcctgggtc gaatcagaca tcgcgaggat cagcccgctc atcaagagaa acttgaaaat
     7321 gataaaggcg atgaccgcaa tcaacaggtt cgccaaaatc cccgcgatgg aaaccatgac
     7381 gttggcaacg tctttgttac gccagcgcag cgggttcacg ggcgtgggtt tgccccagcc
     7441 tatgagcggg aatcgtccgc cacccatcgc gccaaagatg aacccggcga tcggaatcag
     7501 gagcgtgccg atcggatcgg tgtgagcgac aggattcagc gtgatgcgtc ccagcagtcg
     7561 tgccgtgtca tcaccaaact tgtaagacat ccaggcgtgc gccgcttcgt gtgccgagat
     7621 agcaagcagc agcaccacca tgtagatcac gagttgtgga ataagttgtt cgggactcat
     7681 tcagagcctc aacttagcat tccggtcaaa ctatcgcaac aacgtgccct tgatctgcgt
     7741 cggcagactg taaaccgagt cgatcgatac aactgccatc tctccagtcg cactgaacgc
     7801 gaggccgatg agattcacgc ccgccactac cagcttcgca tcaactccgt ccggtgcgat
     7861 gcgaacgatg ccacgacgcc cgcgcaacgc cgccgccaca tacaagttgc cgtcgcgatc
     7921 gaaggcaaga ccctgagggc gcccgagacc tttataaaac atgtcgatgt cgccgtcagg
     7981 attgatccgc cagatcgcat cgaaactggt gactgttggt ccagcgacgt acagcgagtc
     8041 gtccggacca aacgccagat gaaatgctga aactgacggc tcgagttgcg tccacgcgcg
     8101 ctcttcacca atgccattga ctttgaagat cgttcccgtg cgatcgccga tgtgcatcaa
     8161 cccgtcgctg ttgaatgcga tgcctgtcgc cacgccgaga ttgtgcgcaa acgaaactgc
     8221 ctctttaaac ggcgtgatct tgtaaacagt tccttcgagc ctgctggtca caaacatctg
     8281 accctccgga ctgaacgcga tgccggtcgg gttggtgatg tcgcctgagt actcggaaac
     8341 gtcgccactg atgtcgatgc gaaacaacgt caccggcaac tcttcaccac gcgaacccga
     8401 gcgcgtgacg aacagcgcgc catcgtccgg atcaaacgcc ggattcgaga ccggatgaag
     8461 atcgcccgcg agctttcgcg cgaccgtcag gtacgcaggc tcgctcgcct cgtcgccgtt
     8521 ccacaatgtg accggcactc gtccgccctt cgtctcaggc acgatcacca gcacacgccg
     8581 cggcccaagc gcgacaacct gcgccggcgt atcaccgatg agcacaccac acttcgacgg
     8641 ctcacgcgta tcaaggcccg tacaatcaat cacgatctcg ccgcccgcaa tcgcatacgg
     8701 cggatcgaca gctataattt tccctgcgct acccataaaa gttgcacatc ataacattca
     8761 tccacagatt acacagattc cctaaccatc tgcgtaatct gtggatggct tggtatgtta
     8821 taattcgccc ccgcatagga aggaaaaggc tattacaacc gttctaggca acactctagg
     8881 gctcaaagca aatcaactgc ggcgcatcga aaagctgtac actcggcgca tacctcccga
     8941 tcaaatcgtc accctcgagt ttgcacgcca attgggcgaa ttgtcccacg aaacacgacg
     9001 ccaaatcggc gccctgatcg accgccaggg ccacattcag tacgtgatgg tgggcgacaa
     9061 ccgtcgcatc gagctgcctg atttcaaacg cgttcgcgtc gcttcgggga gatttcgcgg
     9121 cctgcgttgc gtccacacgc acctgcgcgg cgagccgctt acgcaggacg acttaaccga
     9181 cctggccctg ctgcgcctgg acctgatggc ggcgatcgac gtcgacgcgg agtcaggact
     9241 gcccggtctc gtccgtgccg cacacttgtt acctttacac gccggcgatc tcgacaacgc
     9301 caccaccccc gagctctacg cctttctcga gccgaagatc ccgtcgcagc tcgatcacga
     9361 cttccgggag ctgatcaact cgctcgaaga agagatggcg cgcaaccgtc gcacaacacg
     9421 ccgcgcggaa gtacgggatc ggactatcct cgtcggcgtc gcgaccggtt cgctggctga
     9481 cgctgaagag tcgatggcag agcttgccga gctcgccacg tctgccgggg tcgtggtcca
     9541 ggatcgaatc atccaacgcc ggcaggcgat cgatccgcgc accgtgctcg gtcgtggcaa
     9601 actcgacgag ctgatcattc gcgcgctaca gctcggcgcg gatttgctcg tgttcgacag
     9661 cgagctgtca cccgcgcaag tgcggtcgtt gtcggaagca acggatctca aagtaatcga
     9721 tcgctcgcag ttgatactcg acatcttcgc gcaacgtgca caatcgaaag aaggaaagat
     9781 tcaggtcgag ctcgcacagt tgaaatacct gctgccacgt ttgatcgcgg gtcaggattc
     9841 ggcgttctct cgtctggcgg gcggcatcgg cggccgaggt cccggtgaaa cgaaactgga
     9901 aaccgatcgt cgtcgcgtgc gcgatcgcat caatcgtctc gagcgtgaga ttgaagcgct
     9961 gcaacaacgg cgacaggagc gtcgcaaagg tcgaacgaga aacgggctgc cgatcatctc
    10021 gatcgtcggt tacaccaacg ccggcaaatc cacgctgctc aacgcactga cgaaaagcga
    10081 cgtacacgcc gagcagcgca tgtttgccac gctcgacccg acttcacgca gactgcgttt
    10141 gccgcgcgag caggaagtga tcatcaacga cacggtcgga tttattcggg cactgccacc
    10201 ggatctgttg tcggcgttcc gcgcaacgct cgaagagatc agcgacagta ccttgttgat
    10261 tcacgccgtg gacatatcaa atccgcgctg ggtgcagcag gttcagtcag tgaagcggat
    10321 cctcgcggat ctgaaactgg ccgagattcc gtcgatactg gcgctcaaca aggctgacct
    10381 ggtcgacgca gagactgtgg aagcggccgt tcggcaggcg cgacagctcg atagcggcga
    10441 gaccgttgtc atctccgcga tcaagccgtc gacgctcgac gcattactgg aaaaagcggg
    10501 cgccgttctg gcccgcaacc tggctgctgc ataaatttga gccgcaaaaa ggcacaaaaa
    10561 gcacaaatgg attacccaag taagacgcat ggaattcaga ataaatcttt tgtgcttttt
    10621 gtgccttttt gtggctaacg ttgcttatgg gacttcgtcc acgattcaaa actccagtca
    10681 gccaggctcg cgataagctg atcgcctacg caatgcggcc gctgatggta ttcaagccgc
    10741 gcacgcgctt tctgatcgcc tgcagcgccc tcgttctcct gaccactctg ctactcctca
    10801 ccaatcgcgg ctccagtttc aatgaaaact acaagctagg tgaagtgatc ggtcgctcgg
    10861 tcatcgctcc cacagatctt actgcggtgg accaggcgga aactgaacga cgtaaaaaca
    10921 tcgccagaga aacctctcgc cccgtcttca attacgactc gagccgcgcg gaaacttcgg
    10981 tacaaagctt tcgctcagac tgggacgatc tcaagacgca agcaaccgaa ggccaaagca
    11041 agtcgctcgt gtggagcggt gaaggtggcg ccgcagtcgc acaggcgatc gccgcgcaca
    11101 agttcaatga agcagatctc gagcgtatcg cctcgatcct ccgcgacagc agtgccggat
    11161 acatttacga cgacgctgag gcggatcgtt tgcaacggga aatcgtcctg atcgacgttc
    11221 gcaattcaag ctcaccggtg atcatgccgg cgccacgaac gagcatgaag gcgctctcga
    11281 cagcgcgccg gacactcgaa caacgcatcg tcaatttacc cgcctggtcg gccgtgcaaa
    11341 agaacgcgct caactctgct ttggcgccgc tgattcgtcc gaacgtggtc ctcgatccat
    11401 ccgccactgc aactgcccgt gaaactgagg ccagtagcgt cgagccggtc gtgatctctt
    11461 tgaaacgcaa tcaggtggta gcgcgggaag gcgacacggt cacgcccggt attctcgcgc
    11521 agatcaatgc catcaagagc tcgggcaatt caggccggcc ctggaacaac ctgatcggac
    11581 tgttgctcgt ggtgattgcg gtttactggg tcgcgtggaa gtttgccgaa cacagaagcg
    11641 gcagcagctt gagtttgagt aaacacaagg cgtttgctct cgtcggttcg gcaattctgg
    11701 tgcagaccgc gctcatgaaa gtgggcttcg cttttggcga cagtatggcg gcgcggatgc
    11761 aggcgccgtt caacgatcca gtcatctgga acttcgccat acctttcgcc gccgccgcat
    11821 tgctcgtcgc gatgctggtc gacacacagc tcgcttttct caccggaatc atcacggcgc
    11881 tattcgcggg aatgctggcg ccgaccggaa tgcagaaagc actcttcgcg atggtttcgt
    11941 gttcgtcagc gatctacggc attggtcgtt atcgcgagcg acagtcagtg actctggccg
    12001 gactactcgt cggtggtgtg aacgcggtga tggctatcgc cctcatcgct tattccgaac
    12061 agccactcac cctccgcacg attctaatgg ccgcggccgc gggtatcacg ggcgggctgc
    12121 tgaccgcggt gtttaccgca ggcggattgc cgatcaacga atcgcttttt ggaatactga
    12181 ctgacgtcaa actgctggag ctttcaaacg ccgatctacc ggtgctcgga cagctcgcga
    12241 tgcgcgcacc cggcacgaac caacactcgc atgcagtcgg gcagcttgcg gaagatgcgt
    12301 gtcgtgcggt cggcgcaaac ccattgctcg cgcgcatcgg cgctctctat cacgacatcg
    12361 ggaaactcgc cgcgcccgag tactttgtcg agaaccagca gggcgacaac ccgcacgatc
    12421 gactgcgtcc gtaccagagc gcgaagatca ttacgagtca cgtgacttac ggtgcgcgac
    12481 tcgcgaaaga gatcggtctg ccccaaaaaa ttgccgactt cattccgcaa caccacggca
    12541 cgcgcacgct tcattacttc ctgcgcaagg cccaggcgca agcaaagccc ggcgagaacg
    12601 ttgatgaagg agactttcgt tatcctgggc caaagccgca gttcaaggaa gccgcgatca
    12661 tgatgctggc tgactcgtgt gaagcggcag ccagatcttt agcgcggccc gacccggaaa
    12721 acattcgcgc gatcgtgctg aagatcttcg attcgattat cagcgacggc cagctcgatc
    12781 agtgtgatgt gacgttgcag gaattgacga ttatacgcga ggcgatgatc agcgcgttga
    12841 ctgcgatcta tcacgcgcgg atcgactatc ccggatttaa cccaccggcc atgactggac
    12901 cactgccacc gctacccgcg ggcaatctcg acagcgaaga gcgcgggatc acctacagca
    12961 aaccctccga agtcccaatc agccgcggcg gtgaagtcga agacgaagcc gtgccacgaa
    13021 gagtcagcaa aagatagcca caaaaaggca caaaaacaca aaaagagatc tgtgcagatc
    13081 cgtgtatgaa atccgtctaa tccgtggcga attttggccg gagttttgag taggcggtgg
    13141 gaaggtcttc tgggagaacg cgcgtctcgc ccacagtcgt cataaaattc gtatccccaa
    13201 cccaccgcgg tagcaggtga atatgcagat ggtcggcgat gcccgcgccc gccgccaaac
    13261 cgagattcat tcccaggttg aagccagccg gtttgtaaac ctcacgcaac gcagcctgcg
    13321 cgcgctttgt ctggtccatc atatcgtccg tgatctcttt ggccatcgaa tcaagctcgc
    13381 tcgtgtgcag gtacgggacg atcatcaaat gcccgctgat gtacgggtag agattcagca
    13441 gcacaaaaca atgcggcgcg cgatgaagca cgtaattttc ttcgtcgtgc tcggggtcgc
    13501 tcgcgatcgc gcaaaaaaca caaacgtcgt tctgcgcatc gatagcagat gcgatgtacc
    13561 tggcgcgcca gggactccac aatctctcca attcaggcac ctcgacttgg ctggagctta
    13621 aaggatgggc gggcgatgag tgtcatcact gaccgggcgg gcagagtttg ccgccgaggc
    13681 aggcgagccg ttatcgtcgt tgatgagttc tttcagttct ttgccgggtt tgaaataagg
    13741 aactcgcttg gccgggatgc tcacgggatc gcctgttttg ggatttctac cgcgccgcgc
    13801 cccacgctct cgcacgcgaa aactgccgaa gccgcgcaac tcaatcttct cgccctgatt
    13861 cagggtttca atgatgctct caaatacgat ctctactaaa cgctcggcgt ctttcttcgt
    13921 aagctcagcc gctcgagcca cgtcttcgac gagttccgct ttagtcatgc attacctcga
    13981 gtgtttgaaa tataacgtct tgataatggc gaggttatgt gactcgtaca ctaccagaaa
    14041 agcgacaggt ggggcaagag tttagccaca gattacacgg attgcactga ttgggatcca
    14101 gtgtaaatcc gtgtaatccg tggctaaaaa agcgtggtta ttggtcgttg tcgggtttac
    14161 tcaatccgaa gtcggccagc tcgccgagcg aggccatacc gccgccggct tccgacgagt
    14221 agatcttcgt gtcgatgatt ggctcgtcct tgccgactgc gcgcgcggac aggccgatct
    14281 tcttggtctc gggatcgatg cgcaggatct tgaaatccat ctcctggccg agctgcacca
    14341 cgtcttccgg cttcgctaca cgctcttccg aaagctccga gatgtgacag agaccttcga
    14401 gattgtcgtc cagctcaatg aaggcgccga agttggcaaa ccgcgccacc ttcccgtgca
    14461 ctacatcacc cggtttgtgt tcgttggtga acttctccca cgcgttcggt tcgaggtctt
    14521 tgatcgaaag cgacagccgg cgattttccg tgtcgatgct cgtgattacc gcatcgatct
    14581 cctgtccctt cttcagcact tcactcggat gcttgatgcg tcgcgaccac gaaatgtctg
    14641 acacgtgaac aagaccgtca accccatctt caatctcgat gaacgcaccg aagtctgtca
    14701 gatttctgac gcgtccatga acacgcgtgc cgacctggta acgctcgtgc agcgtctgcc
    14761 agggattctc ctgcacctgc ttcatgccga gactgatacg tcgcgctctc ggatcgacac
    14821 tcaacacttc aacttcgacc aggtcgcccg ggttgacaac cttgctcggg tgcttcacgc
    14881 gcttggacca gctcatctcc gaaacgtgca cgaggccttc cacgcccggc tcgagctcga
    14941 cgaacgcacc gtagtcagcg acgctcgcca cccgtccgct tacgcgagta ccaaccggga
    15001 aacgctcctc aacactggac cacggatcgg gcagcagttg cttgtaaccc agcgacacac
    15061 gctcgcgatc gcgatcgaat ttcagcacct tcacctggat cgtgtcgccg actttgaaca
    15121 gctcattcgg gttct
//
LOCUS       DASSFQ010000238         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1741173, whole genome shotgun sequence.
ACCESSION   DASSFQ010000238 DASSFQ010000000
VERSION     DASSFQ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741173"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFH96_08675"
     CDS             complement(<1..507)
                     /locus_tag="VFH96_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HET6974082.1"
                     /translation="MTCPKCGAQLLEGSTFCGACGQRLAPTATQPMNQSAGRGIQIDE
                     DSRGRGRGYDYEVLHQPSFSLAVLRLQAEQSIQAESGAMVSMSANIELQSQMKGGLFG
                     AIKRAAGGESAFISTFTARGGPGEVTLAPGAPGDVAAIELTGQPFFVQSSSYLAGDAS
                     LTVDTKWGG"
     gene            complement(558..1901)
                     /gene="asnS"
                     /locus_tag="VFH96_08680"
     CDS             complement(558..1901)
                     /gene="asnS"
                     /locus_tag="VFH96_08680"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET6974083.1"
                     /translation="MTTQLHEITSDKHTYINQLSNHVGAEVILKGWLYNLRSSGKIAF
                     PQLRDGTGVVQCVVLKTAISPELWEQLKGLGQESALMIKGTVRADSRAPGGYEIDVTD
                     AEVLQEVNDYPITPKEHGTEFLMDHRHLWLRSRRQHAVLKVRHTVVKAVRDFLDNDGF
                     TLMDSPIFTPAACEGTTTLFEVDYFDGEKAYLTQSGQLYNEATAAAFGKVYCFGPTFR
                     AEKSKTRRHLTEFWMVEPEMSYATLDDVMNLSERFLSYIAARVLDQRSEELKVLERDT
                     SKLEAIVPPFPRVHYDDAVKILHEGFEKGAMEAKFEWGGDFGAPDETYISSQYDKPVM
                     VHHYPAEVKAFYMARDPERPELALGVDVLAPEGYGEVIGGGERATSLEFLKEQIALHQ
                     LPQEAFEWYLDLRRYGSVPHGGFGMGIERCTAWMCGIEHIRETIPFPRMLYRIRP"
     gene            complement(1923..>2895)
                     /locus_tag="VFH96_08685"
     CDS             complement(1923..>2895)
                     /locus_tag="VFH96_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6974084.1"
                     /translation="FITEQGIASSDVIPLTPDASTRNYFRIRWKKSTAVAAVYPEPFD
                     PEFHPYLDVTRLFLDCDIPVPEIYAVDGTAGIIVQEDLGDRQLFHIYDESEELCDEYK
                     EKAIALIAKIQKATAHAYETQSIASRLAFDEAKLSWELDFFFDHYFGSLRGETPRHAE
                     VAELKAELNDIAAELAAAPRVLCHRDYHASNLMIDNKKRLRVVDHQDARMGPASYDLV
                     SFLLDRQPCPPSLAELRAYRLFFLEQRRVLGLGAIDPDDFALEFRLMTLQRGLKATGT
                     FSYQTAVCGRGTVYQHFIQPTLEIVLQAAEWLERFPTLRRMLKERIN"
BASE COUNT          641 a          880 c          750 g          624 t
ORIGIN      
        1 gccgccccat ttcgtatcga ccgtgagact cgcatcaccc gcgagatatg aactcgattg
       61 cacgaagaat ggctggcccg tgagctcgat tgcagctacg tcgccgggtg cgccgggcgc
      121 gagcgtgact tcacctggac ctccgcgtgc agtaaaggtc gagatgaagg ccgactcgcc
      181 acccgccgcg cgcttgatcg cgccgaacag cccgcccttc atctgtgatt gaagttcgat
      241 atttgccgac atcgaaacca tcgcgcctga ctcagcctgg attgactgtt ccgcttgcag
      301 ccgaagcacg gcgagcgaga acgacggttg atgtaaaact tcatagtcgt aaccacgccc
      361 acgtccgcgc gagtcttcat cgatctgaat cccgcgcccc gcggattgat tcattggctg
      421 cgtcgcggtc ggcgccagtc gctgcccaca cgctccgcag aacgtcgatc cttcaagtaa
      481 ctgcgcccca cacttcggac aagtcataac tcaaactccc catccgtttt caaatccgta
      541 aaaatccgtg gctggtttta tggtcgaatc cggtacaaca tccgcggaaa cggtatcgtc
      601 tcgcgaatgt gctcgattcc acacatccac gccgtacaac gctcaatccc cattccaaac
      661 ccgccatgcg gcacgctccc ataccgccgc aaatcgagat accactcaaa cgcttcctgc
      721 ggcaattgat gcaacgcgat ctgctctttc aaaaactcga gcgacgtcgc ccgctcacca
      781 ccgccaatca cctcgccata accttcgggc gccagcacgt caacaccgag cgccagctca
      841 ggtctttccg gatcgcgcgc catataaaag gctttcactt cagccggata atgatgcacc
      901 atcaccggct tatcatactg gctcgagata tacgtctcat ccggcgctcc gaaatcaccg
      961 ccccactcaa acttcgcctc catcgcgcct ttctcgaatc cctcgtgcag gatcttcacc
     1021 gcatcatcgt aatgaacacg cggaaacggc ggcacgatcg cttcgagttt tgacgtatcg
     1081 cgctccagta ctttcaactc ttcacttctc tgatcgagca cacgcgctgc aatgtacgat
     1141 aaaaaccgct ccgacaaatt catcacgtcg tcgagcgtcg cgtacgacat ctcaggctca
     1201 accatccaga actcagtcag gtgcctgcgc gtctttgact tctctgccct gaacgtcgga
     1261 ccgaagcagt aaactttccc gaaggctgcc gctgtcgctt cgttgtaaag ctgccccgac
     1321 tgcgtgagat acgctttttc accatcgaag taatcgactt cgaagagcgt cgtcgtgcct
     1381 tcacacgctg cgggcgtgaa gatcggcgaa tccatgagcg taaaaccatc gttgtcgaga
     1441 aaatcccgca ctgccttaac aaccgtgtga cgaaccttca gcaccgcatg ctgccggcgt
     1501 gagcgcagcc agaggtgcct gtggtccatc aggaactccg tgccgtgctc cttcggagtg
     1561 atcggataat cgtttacctc ctgcagcact tcggcatcag tgacgtcgat ctcgtaacca
     1621 ccgggcgcgc gactgtcagc gcgaacagtg cctttgatca tcaacgccga ttcctgcccg
     1681 agtcccttca actgttccca cagctcaggc gagatcgcgg ttttcaaaac aacgcactgg
     1741 accacacccg taccgtcgcg cagttgcgga aaggcgatct tgccgctcga gcgcaggtta
     1801 taaagccatc ctttaaggat tacttcagcc ccgacgtggt ttgaaagttg attgatgtag
     1861 gtgtgcttat cggaagtaat ttcgtgtaat tgagttgtca ttgaaaaacc gaagcgtgca
     1921 aatcagttga tacgttcctt taacatccgg cgtagagtgg ggaatctttc aagccattca
     1981 gcggcctgca aaacgatttc gagtgtgggt tgaatgaagt gctggtagac cgtgcctctg
     2041 ccgcagactg ctgtctggta agagaaggtt ccggtagctt tcaggccgcg ctgaagtgtc
     2101 attaacctaa actcgagcgc gaagtcgtcc gggtcgatcg cgccaagacc gagcacacga
     2161 cgctgttcga ggaaaaacaa tcgataggca cgcaactcag cgagcgacgg cgggcacggt
     2221 tggcgatcca gcagaaacga cacgagatca taacttgccg gacccatgcg cgcgtcctgg
     2281 tgatctacaa cccggagacg cttcttgtta tcgatcatca aattcgatgc gtggtagtcg
     2341 cgatgacaaa gcacgcgcgg cgcggctgca agctcggcgg cgatatcgtt cagctccgct
     2401 ttcagctcgg cgacttcggc gtggcgcgga gtctcgccgc gcaggctgcc gaagtaatga
     2461 tcgaagaaga agtcgagctc ccaggaaagt tttgcttcgt caaaggcgag ccgactcgcg
     2521 atcgactgcg tctcataggc atgagccgta gccttctgaa tcttcgcaat cagcgcgatt
     2581 gccttttcct tgtactcgtc gcagagctct tccgactcgt cgtaaatgtg aaatagctgc
     2641 cggtcgccga gatcctcctg cacgatgatg ccggcagtgc cgtcgactgc gtagatctcc
     2701 ggtaccggaa tgtcgcagtc aaggaagagg cgcgtgacgt cgagatatgg atggaattcg
     2761 ggatcgaacg gctccggata cactgcggct acggcagtgc tctttttcca gcgtatgcga
     2821 aagtagtttc gggttgacgc gtcgggcgta agcgggatga catccgaact cgcgattccc
     2881 tgctcagtga tgaac
//
LOCUS       DASSFQ010000239        30825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1306462, whole genome shotgun sequence.
ACCESSION   DASSFQ010000239 DASSFQ010000000
VERSION     DASSFQ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 30825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30825
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306462"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VFH96_08690"
     CDS             complement(<1..1223)
                     /locus_tag="VFH96_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HET6974085.1"
                     /translation="MNHLSSETFKENAKAALADVQLRGALKNATSLFGERRKEAAASL
                     PNWEDLRSQARAIKDEVLLHLDRYLEEFVRNAESRGAKVHWARDAAEANQIICGLATQ
                     RAARIVVKSKSMTTEETHLNDALEAAGMQVVETDLGEYIIQLANEPPSHIIAPAIHKT
                     RHQIGALFTAELGMPPTDNVDQMTSTARAKLRDRFAAADVGISGVNFAIAETGTIVIV
                     ENEGNIRLTTSLPRLHIAVMGIEKVIPRFNDLDVFLKLLPRSGTGQRLTTYQSFITGT
                     KRAPADEGPDELHIVLLDNGRSRMLAHPVTRQSLACIRCGACLNACPVYQQVGGHAYG
                     SVYPGPIGAVITPQLMGIEKAAQLPYASSLCGACREVCPVKIDIPRLLLHLRGEITER
                     QGSARERLVFKMWAKV"
     gene            complement(1204..1935)
                     /locus_tag="VFH96_08695"
     CDS             complement(1204..1935)
                     /locus_tag="VFH96_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET6974086.1"
                     /translation="MRVSLFVTCLVDQMWTSIGVSSVEVLRRVGCEVEFDKRQTCCAQ
                     PAFNTGYLDEARLVAKRFISIFEESKADAIVSPSGSCTAMVHHYSQLFAGDSQWLSRA
                     HAIAAKTHELSSFLVRVLKKEDVGASWNGRLTWHDACHGLRDLNIKSEPRALIRKVRG
                     AEFVELEHADSCCGFGGTFSVKYPEISVAILDQKIDAIERAGVQAVVSGDASCLMQIG
                     GRLSRKGSKVRVMHLAELLNESSQQ"
     gene            complement(1984..3153)
                     /locus_tag="VFH96_08700"
     CDS             complement(1984..3153)
                     /locus_tag="VFH96_08700"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6974087.1"
                     /translation="MSAPVPGHTMKASVLCDVQRLEVRDIPRPAISPYEVLIRVAAVG
                     LCGTDVHIFAGHANYNTNEYGQAIPLVLQPQILGHEITGVVEQVGSAVSDLREGDRVV
                     IDQGLNCVSRRREVLCEYCRTDNSHQCEFYREHGITGLPGGLAEFIAVPAVNAIKITA
                     PLETSTAALVEPLGCIIHSSDMVAKARQSEQRRVRSVLICGAGPAGLLFTQYLRNVLR
                     YEGLLFVSDPNERKRQLAKSLGADEAIDPAGADLVEVVHEHTGGKGVEYLIEASGSGE
                     VFRSMPGLICKQAVVLLYGHGHAGTDLSVLSSILFKEPVLVSSVGASGGFESDGRPAV
                     YRRALNLIEQQRIDIAPLITHRYTSFSDVEKALSSDIHTDDYVKGVVTLNADFRG"
     gene            complement(3183..4148)
                     /locus_tag="VFH96_08705"
     CDS             complement(3183..4148)
                     /locus_tag="VFH96_08705"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6974088.1"
                     /translation="MPETYFITGAQGCIGSWIVKALTERGDTAVVFDRSDDSRRLSAI
                     INDDDLAHVRFITGDITDQAAVLSALNDSGAARVIHLAGLQVPTCKADPVTGALVNVA
                     GTLNVFEAARKIGLNGIVYASSAAVYGMNDDDLALDESALCEPTTHYGVFKRANEGNA
                     RVYFLDHGINSVGLRPLTVYGVNRDTGLTSDPTKAMKSAVLGRPFHIRFGGATDFQYV
                     ADTAATFIACADNMPEGAHVFNLHGETVTVERIAKFINASSNGDLITFGGPPIPIAAA
                     MDDSAIRRLINDLPSTPLEIGMRETMDRFAALRDAGRLDTSDLTL"
     gene            complement(4148..4954)
                     /locus_tag="VFH96_08710"
     CDS             complement(4148..4954)
                     /locus_tag="VFH96_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6974089.1"
                     /translation="MIDAHQHFWQPRRFDYPWMSEDLGVLYRDYLPADLAPILQQNHI
                     TKTVLVQASNSIAESHWLLELAESNDFIAGVVGWVDLTNPEIPAHPKFKGVRHLVESE
                     PNDDWLVQPRILSGLQKLSAHGLSYDLLVHTRHLKHVPLVAESCLDLKLVIDHLAKPP
                     IARNEIDEWSRAIKPLARYRNIHCKLSGLVTEANWDSWRVDHLRPYVDCALESFGTDR
                     MMFGSDYPVCLLAASYDRVLDGFLEILKALSDADREKIFANNAAKFYRLD"
     gene            complement(4987..6318)
                     /locus_tag="VFH96_08715"
     CDS             complement(4987..6318)
                     /locus_tag="VFH96_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET6974090.1"
                     /translation="MNRRNYLALMGAAAGALATSKLASAQDPKPAEVSAPTTSTIEDR
                     TRRMKWWHEAKFGMFIHWGLYSTLGRHEWVMENEGIPAKEYEQYANNFKPKPNCAEAW
                     AKLAKRAGMKYMVMTTKHHEGFCNFDSKLTNYCATKKAPGRDLVKEYVEAARAEGLRF
                     GFYYSLMDWHHPDGAHCLKDEAARRRFVAYIHGQVRELMTNYGKVDILWYDVPWPLDA
                     KGWESVEMNTMVRTLQPDIIINNRSKIPEDFDTPEQRIEASANRPWESCMTLNDSWGY
                     HISDRNWKTPTTVIRNLITCARDGGNYLLNIGPTEDGSIPLESVKVLSTVGDWMAKHG
                     DTIRLSDNCQPRRGNYLSYTRRGNTLYAHVHYWPGETVVIANLLNKVNSVTMHSTNTP
                     VKFEQDDFRIRLVGLPKNAPELVTTFALECDSEPKQSHEGKRAEKPRDVSA"
     gene            complement(6387..7289)
                     /locus_tag="VFH96_08720"
     CDS             complement(6387..7289)
                     /locus_tag="VFH96_08720"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF002958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HET6974091.1"
                     /translation="MHPNTLRKKLSGVIAFPVTPFKEDLSLDLPGLHENLTKLLEHPI
                     AAIVVVGGTGELYSLTPAEYTRVVELTALAIEDRVPVIAGVGFGQRLAVEMAQAAEKA
                     GADGILAFPPYYPQAEDDGLFEYYRAIGKATRLGMLIYTRDWASFSPSLVERLTAIPT
                     LIGWKDGQGDIRRLQMIMNHVGDRLHWIGGAGDDMVAAYFSIGVTTFTSSIATVAPQL
                     SLKLWELADTGDSETLRDLLDRCVVPLYALRARRNGYEVSAMKAMMDMVGLSGGPVRP
                     PLVNVKPEEEDELRTILACWEKFL"
     gene            complement(7308..9065)
                     /locus_tag="VFH96_08725"
     CDS             complement(7308..9065)
                     /locus_tag="VFH96_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HET6974092.1"
                     /translation="MISRRQILQLAIVSPIIAAFPRLQNAPSKVIPLVDGTDVSRIKI
                     DRGRTRIKNHGRDAVKIKEIVLFDLKLSFPPGTKLYGEGFQMLSQTGGSLGQPADLGN
                     YTDAKHYKLPAPPNTKACYGMMMLSPAAHDHHLFAFTSCRRFMGQFYLNYPSLQVVLD
                     IEGRELRPGEEWQLESFTYKSGNDREQLLDQLATQLSANHPPLKFPSPPTGWCSWYCF
                     GPRVTAQQVRDNLDFIAKHTPGLRYVQIDDGYQPAMGDWLETGAAFGGNVQGVLKEIR
                     ARGFEPAIWVAPFVAEEKSQVFQQHPDWFVKDADGAPLQSDRVTFGGWRRGPWYVLDG
                     THPEVQQHFEKFFRTMKREWGCTYFKLDANFWGAIHGGRFHDQRATRIEAYRSGMRAI
                     LRGAENSFVLGCNHPIWPSLGLIHGSRSSNDIKRTWERIETTARQNLSRNWQNGRLWW
                     NDSDAIVLTGDLPDNEFQFHATAIYASGGMLLSGDDLTKIGAEKLAMLRKLQPPTGVA
                     ARFDDDSFRVGRVQLKDREVITLMNWNDAPQDISIRLPRRCRLTDFWSEADLGIHSET
                     FEIKNMPARSGRLLICVHP"
     gene            complement(9143..11449)
                     /locus_tag="VFH96_08730"
     CDS             complement(9143..11449)
                     /locus_tag="VFH96_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HET6974093.1"
                     /translation="MHDGTNKDLMVMTLGEVSTPIADGTFNPTKDELRLNDGAVIKNY
                     FRDTLKIKYFQPIDKTRFPLPPSGWCSWYFFYQEINEDEIKLNAKWIAENLKDFGVQY
                     VQIDDGWQGTGHGLGENRDWSTINKRFPSGMDGLAAYIKSLGLKPGIWLAPHGQSNEA
                     LVKNHAGVFMMKPDGTTASNTWEGTFLVDPSAPESQQYLKDLFAKLSSWGYEYFKIDG
                     QPIVVREYRNKKSFMKNPADDTDALYRETLKSIRAGIGPGSYLLGCWVVPLEGVGLMN
                     GSRIGADVLPNWDGFKFAIRATMQYYFLHNVAWYTDPDVFIVRAPLPIEQARAWATLQ
                     GLTGQAALMSDRLVDLSADRVDLIKRVYPAVDIRPLDLFSSDRNKRIWDLKINHLGRA
                     YDVVGVFNFDESKPSSSYLSWKDLGLLEDRPMHVFDFWNKEYLGAFEKGLSLELSPAS
                     TRVLSLVPATDQIQLVSTSRHITQGWVDLISQSISGNRYTGKSKLIRNDPYNLCFAFP
                     RGKNFRIKSATASGLPVRIVNHQGWAEVEITPAKTTEVRWEVVFEPAPAYHFPVKEPQ
                     RVWAERYGVDGANLRWVIPAQPTFAYQVSLNGTVLGYTTSQTFALRGLDPNTNYTAEV
                     RTVWQDGTLSEKKAQLSFTLKKIQPPEIFLSDLEPVRLTPGWRQPELNRNVNSISMPT
                     NSEIEFELNGTYDSFVALVRIDHEVNNSKAKVEFSVIGDGQELWKSDTSQSLKIDVKN
                     VKRLMLRVRRVGEGGRVYVDWVDAKLTR"
     gene            complement(11561..15199)
                     /locus_tag="VFH96_08735"
     CDS             complement(11561..15199)
                     /locus_tag="VFH96_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET6974094.1"
                     /translation="MFSFLRCNAAILAVIIVALAFGTVATAQTITGSISGAVMDQTGG
                     MIPGATVTLTSEKTGQARGSTSDSEGRFNFAALQPGAYALKIERVGFQTLEQRGVILS
                     ANENLALGDLKLQPGQVSETVSVMSEGTIVERESSDLTARLTADQINLISTKGRDVTS
                     LLRLLPGTSNNDDIEGAGDGFGTDLPNISGQRGRSTVPTIDGLFAGEPSGSNKLSMTI
                     NQDAVAEVKVLRNNYGAEYGNNGGAIINIVSKGGGKDFTGSVYYFLRNESLNATPFFN
                     NKAGLKKPLYRHLYPGGNIGGPMWLPKFGEGGNFWLKDKAFFFFAYEKPHQITPQDPR
                     FVTMPTALERNGDFSQSINSANQKVFVKDPLLPGGCAASDTSGCFRDPSRATASNPLG
                     LNIIPQTRFNASGFALLNYFPLPNTFGGSGGSAFNYVSQSPQDVPKRSQVLRFDIKPS
                     NNDTIYWKYQWWTSDNLGTGTSGWPGNDNNRWGINSHYLYKDDGWSANWVRVINASFV
                     NEFNFGMRHDSEGFVPGDGEIERVQRGALNYTAPQLFPQNNHLGTIPRVTNWGGVRGP
                     TNGVANINWLDRWGETGNDYIKPSFADNLSITHGDHALKFGVYFERLLNGEAPGGQWS
                     GVFNFAGNDSNYTAALGNTSYAYANALIGNFRNYQESTARPFTNLRLTQVQWYAADQW
                     KIHRRLTLNYGLRFGYHSPFEQIDGQGSNFVQGLFNRSRAVALYESACAVTFTPPATC
                     PAAQRRARNPVTGELIPLVGANAGLVGAIVPNSGDANNGLALGIDPKTTPGYRNTKAI
                     DYEPRFGLAWDVFGSGKSVLRFQGGVYHAPRVGGGTTGGNLVNNQPANRTFSIDFGNI
                     NNLASLTGTALTRPSALNAVEEKSHTPAIYNFTFGIQQDIGFQTVMEVSYVGSFARHL
                     GQRININGVPDGARLGTNNIDPVTGQRIGNDFLRPYRGYADITMVTWAGTSNYNSLQA
                     QITRRYTKGFQYGLAYTYSKSFDYANDDSSDVFFNRPYKDFNYAPSDFDQTHILTINY
                     IYDIPNLSRKFNNGFVKAIFDQWQLTGTTSYASGKPKNISVTYNGGVTDITGGQDNAR
                     PNTICDPMRNISGSDPTGTPYVVNVTCFARPTRLGDIGNTPRNSLRMPSIFNNDIALF
                     KNIPMGESRALQLRWEVYNVFNHTNFRDIDAGLVFDAAGNQTNTRFGAPIAARTPRVM
                     QAAIKFSF"
     gene            complement(15362..16147)
                     /locus_tag="VFH96_08740"
     CDS             complement(15362..16147)
                     /locus_tag="VFH96_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET6974095.1"
                     /translation="MTNSTVPKLKVVQPLRSAPTTEQVVERVYELIKRQDLQPGERLP
                     PERELSKQLGISRPALRAGLSSLISMGVLQSRQGAGTFICDGPPALDSEPLRLLAQLH
                     GFSFEHMFETRSVLEVGAAGLAAERATGEQLATMSEQIADMFAALKDPQQFLVHDVAF
                     HRAVAAASNNPTLATLVEMVSAILYERRRETIERAHDFNEALELHRRVYRAIRARNPE
                     EAREAMREHIVRAQKAFALEEAQNRTERGSAGSNSFTSRDKET"
     gene            complement(16257..17456)
                     /locus_tag="VFH96_08745"
     CDS             complement(16257..17456)
                     /locus_tag="VFH96_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET6974096.1"
                     /translation="MKLIAVIALLLLIPFSNIAQPRTDWSVATVESTMKRYPTAKDLG
                     SWGYAKSLYLYGQYLVYKRTHDPRYLKYIKDWVDMHVDDQGVVRSANAQGEVQEIKFD
                     NLDSMLPGNLLLLLYQETKEQKYKLAADRIRKRFDSYPRTKDDGFWHATSKSREWQLW
                     GDGVFMSMPFLVRYGNMFGDAKYANDEAVLQLLTYWLHLNDIYTGLMFHAYDESGQSA
                     WADKETKHSAEKWCRAMGWFGMTLIEVLELLPRNHPQRPLLIGHLSLLVKAWARYQDE
                     KTGLWYQIVDKGNDPANWFETSSSSMYTYVTAMAVDRGYVEKKYAAGVNRGYAGVLTK
                     ISLDKDGQTNLIDICEGTNVADLAYYYARKRNTNDFHGLGAFLIMNEKFMNKTLNGSS
                     RTLWQPR"
     gene            17620..18252
                     /locus_tag="VFH96_08750"
     CDS             17620..18252
                     /locus_tag="VFH96_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6974097.1"
                     /translation="MTKSRDNRDDHDPGRRKFLKGVGIAGAGAAIADHLWAEAEAAEP
                     PATPESLSGSVKVVLDVNGQKREAQVEPRTTLLNTLRNHLDPAVTGPKLVCDVGTCGA
                     CTVLLDGKPVYSCLVLGVDATNRKITTVEGLGTPENPNAVQSAFVENDALMCGFCTPG
                     FVTTISAYLKKNPNPSLAEVREACKGNFCRCGTYPRVFEAALAAAKGSQA"
     gene            18265..20511
                     /locus_tag="VFH96_08755"
     CDS             18265..20511
                     /locus_tag="VFH96_08755"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6974098.1"
                     /translation="MQQQQPQAQPSPSPKPKKKIRVPRVVNGVEQMVEIEVDADTGPG
                     WGPNDKHKLLNQRYTRVDGPLKATGVAKYTYDQRVPGMLYARILRSPHAHARVTKLDT
                     DAASKIPGVKAIIPAPLTEVRFAGAPVAAVAATTPEIAGDALRAIKVTYEVLPHVVHA
                     HHAIRPDAPKVLADEENLMERGKNGDAQKAEAAFATADAIVEGEYITSRVHHVCLETH
                     GMVVDYRGGDSATIYASTQGTFTIHEDAAKELGLASNAVTTTVEHMGGGFGSKFGLGL
                     EGMLACRLSKQTKAPVKLMFTRYDEFVMAGNRSGSWQKLKAGVKNDGTIVALQARQYR
                     LGGLGQGSQAGQPFIYRMGDTYREVYALHTNEDSSVAMRAPGFPQASFAVETLVDELA
                     YKIKMDPVEFRKKNLRDEVYHRQLDRGAREIGWSRRNPVAGGNAGPLKRGMGCAVGTW
                     GGGGNNQCKVDVTIARDGSVLVAVGTQDLGTGTRTYMRAIVAEELGLGIKDVKERIGN
                     SKLGAANPSGGSTTAPSLSPSVKDAAYKARLLMAERVAPLLGNPKPEDVVFADAKVSA
                     NGKTLSWQQAVAALPAAGITSHGEWRVDLQARGIHGVCFAEVEVDVETGHVKPIKMVH
                     IQDGGLPLNRLTMESQINGGMIQSLGMALWEGRVMDAQLGMMLNPGMGDYKLPGSLEM
                     PEMVPLIDDEDKREAVIGIAEGCIIPALGALANAVFNATGVRVRETPITPDKILMGLM
                     NSTALSRG"
     gene            20538..21539
                     /locus_tag="VFH96_08760"
     CDS             20538..21539
                     /locus_tag="VFH96_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET6974099.1"
                     /translation="MKSFEWTNPASVGDAVKMLAVSTPGDLDDAPRPIAGGQDLLTTM
                     KDYTSRPVRVVNLKNIRGLDKITLNARGLTIGALVTLSELEEHAGVRKNFPGLAEAAH
                     SIATPQIRNLGTVGGNLCQRPRCWYFRLEEVNCLKKGGSECYAANGENKYNAIIGGGP
                     SYIVHPSDLAPMLLALGASVTVVGAKGKRSIPLDKFFTLPSEGNIRRENVLKNEEIIT
                     EIVVPSSPVAARSTYLKFKERESLDFALASAAVAVQLGANREVKDARIVLGGVAPIPW
                     RVPAAEKFVIGKSLNPEVLAETAKIALAEARPLEKNAYKVPLAQTLVRRALAKAGTV"
     gene            21555..21788
                     /locus_tag="VFH96_08765"
     CDS             21555..21788
                     /locus_tag="VFH96_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974100.1"
                     /translation="MANELVEPMQSAFCGSLRSKKYYMLDRLATSADEYLDAANHVWC
                     CETQEVIGPDNARVDPERCGPGRACYTSALKSV"
     gene            21809..22465
                     /locus_tag="VFH96_08770"
     CDS             21809..22465
                     /locus_tag="VFH96_08770"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET6974101.1"
                     /translation="MRKGLLAVAAMLVAVSALVFSSQAENVSLRGDYVEVRTASVFAG
                     ACHYNGEVVTTGRDAMMAWNVTSGKWQGVDLSGVRVLAIVSSDANLSESNAARQSEII
                     IDSNASRTQALAMVNALKEKYAASLGNVVEVRSAPISFARNGREYAVVTNEAAINVEA
                     MPNDLCCKMPNLVWYAPLVGLENRKVGYTSKALYSGKVVGEPWSRSGENSAFYGTFSL
                     "
     gene            complement(22491..24875)
                     /locus_tag="VFH96_08775"
     CDS             complement(22491..24875)
                     /locus_tag="VFH96_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6974102.1"
                     /translation="MKPISRRAFLQTASTALVAGTLLGKPASYLGETSAPYLLSERLR
                     EWEYRRGGLGGPWEAWRKANDDANVWTKVEVPHCFNAFDAVDPDVPYYQGPGWYRTRL
                     AIKNPYPSGRTLLHFEGAGQKTEVFIHTDKVGSHVGGYDEFTIDITDQVNARPKILGE
                     AAVPVAVMCDNSRDLEMIPSNLSDFNLYGGLYRYVNLVYVPALSIERVHVVPELLPNG
                     KARVTVRARLYNPTGIKDEVELEVKVKGPDNEVVHSKTSKLNPWTGTLDLTTFVIDSP
                     SLWSPGKPSLYLCVVTVANEPGISKIAAQFGVRSFEFVKNGPFKLNGERLLIRGTQRH
                     EDHAGVGAAMTEDLMRKEMKLIKDLGANFIRLGHFQQSRTILDLCDKLGLLVWEEIPW
                     CRGGLGGERYQEQARRMLRNMIDQHRNHPSVIIWGLGNENDWPGDFESFDKDKIRAFM
                     IELNTLDPSRKTAIRRCDFCKDVVDVYSPSIWSGWYQGKYTEYKTRVQTEIKKVDHFL
                     HMEWGGDSHARRHSEASEVFARAPQEGDWSETYICDLFDWHLKEQETMPELTGSAQWI
                     FKDFATTLRPGNPVPRVNQKGLVERDLTLKEGYFVFQSYWAEKPMVHIYGHTWPVRWG
                     APDEAKLVKVYSNCGKVELFVNGVSAGVKKRNSQDFPAAGLRWLVKFKAGENVVRAVG
                     KNAEDEIRFVYQTEKWDKPAKLVLNVSNSIVEAQLVDAKGVLCLDARNIVRFGITGDG
                     RLIDNLGTSSTSRKVELYNGRAQISVESKGQLVLSVSADALPTAFLSTDYTDKH"
     gene            24945..26750
                     /locus_tag="VFH96_08780"
     CDS             24945..26750
                     /locus_tag="VFH96_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974103.1"
                     /translation="MNAYLSIAFILLFQNGHIDLRKTALKSTTPSDEIVRLDINHDGK
                     PDILERWWNGKRVRWLDENGDMLPTDTRGDQVGDVMQIDKNGDGIYDSETDINIKWAD
                     NDGDGRADLQAFVTQGRAWSNGNFDPAESHWMVFIDVEKDGVLGWVDWTKYDFGNDNW
                     GYTGTTDWLPDYNGNAIFLKVHRPPQSLPDPRLNWENPFAFFDFDGDGASEMAIRWLD
                     PVPPLDKDVTKLSGVLSEAFATFDLDNDSTKGNETDYDMSLRGAGGPGVPYRQFVHKY
                     PALKGNPKFDGCFQWNNWRQIDELMYMPHEKSYDEFFNARWATMYFVFDEDDDDHRWE
                     RVEMYYPMHGFGGPKDIDLYSTKKWRRGNYAELAMAGPNEKPGLSGHPQADSLGDRGE
                     FDEDNSGGGKLYVGVFDRKLHLAGAEWGAWTVDKNAEYHGGSKTPSPKPIAPRVEELV
                     KYTDTDNNGFLDTIEYDYDGDRTIDLRVSLLDYRTPSNPHPDVVPLIDTHKEGWKGLN
                     VLFGQIASQSFQEGLTVYRAAWRRGLTTPEIDKLASASAIGERYDHGYWIKEKIFREI
                     RKRVRGTKLEKDLTKLYYTGQFEEYARRIAEVPAK"
     gene            26893..28065
                     /locus_tag="VFH96_08785"
     CDS             26893..28065
                     /locus_tag="VFH96_08785"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET6974104.1"
                     /translation="MPQNRVSIFDTTLRDGEQSPGCSMNLTEKVRMAGKLQDLGVDII
                     EAGFPIASDGDFAAVKAIAAQCRHVKVAALCRTAEQDVARAAEALKYAARPRIHTFCA
                     TSDIHLEHKLKKTRAEVIAMTRAAVRQARSYADDVEFSAEDATRSDIDYLCEVLDAAV
                     DAGASVLNVPDTVGYTTPNEYTHLVQQVRERVVRDRDDVIISVHCHNDLGLAVANSLA
                     AIEAGARQVECTINGIGERAGNASLEEIVMALHVRADRLPFETGIKTTELYPTSQLLS
                     SIVGIDVQPNKAIVGRNAFAHEAGIHQHGVINNPLCYEIMTPESVGVPANSLVLGKHS
                     GRHALSLRYAELGYDVTAAELDAAYMRFTNLADRKKNIYDQDLISLIGNTHNVVAA"
     gene            28077..29174
                     /gene="leuB"
                     /locus_tag="VFH96_08790"
     CDS             28077..29174
                     /gene="leuB"
                     /locus_tag="VFH96_08790"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET6974105.1"
                     /translation="MQARITILPGDGIGPEVTTEAVRVLQTIAAIQGYRFEFEEHAIG
                     GVAIKRFGSPLPRTTLDAALASDAVLLGAVGSPEFDRVPPNQRPEAGLLLLRSALGGY
                     ANLRPAIAYSSIAACSPVRTEVAHDADILIVRELLGGLYFGEPRGIDADVATNTMRYS
                     VEEIERVARVAFEAARKRRGKVTSVDKANVLETSQLWRQTVIRVSQDYPDVMLEHLYV
                     DACAMHLIANPRRFDVLLTENLFGDILSDEAAVITGSLGMLASASIGGEVGLYEPVHG
                     SAPDIVGQNAANPCGAIASAAMMLRYSFDLEQDAKDIELAIQDVLEAGYRTPDIAEGT
                     GGYVVTTSEFGELICQAVTEIADMRHAYHAV"
     gene            29260..30627
                     /gene="leuC"
                     /locus_tag="VFH96_08795"
     CDS             29260..30627
                     /gene="leuC"
                     /locus_tag="VFH96_08795"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET6974106.1"
                     /translation="MPTLFDKLWQSHVVREARGEPTLLYIDLHLIHEVTSPQAFDGLR
                     QAGRKVRRPDLTFGTVDHNVPTTDRSLPIADAIAANQIETLRRNCSEFGIQLYDIDSP
                     EQGIVHVIGPELGLTRPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLPQN
                     KPLTMKVETRGALLEGVAAKDLALGIIGKIGTDGATRCVIEYAGDAVRALSMEGRMTL
                     CNMSIEAGARAGMIAPDETTFAYLKGRRFSPEGDVNYWKTLHSDTDAKFDRTVEIDAA
                     QLEPFVTWGTSPGMVVPVTSYVPDPADEAAERALEYMALKPRTNIEDITIDRAFIGSC
                     TNARIEDLRAAARVVKGYRINSSVSAMVVPGSQEVKRAAEREGLDRIFHEAGFEWREA
                     GCSMCLGMNADILQPGERCASTSNRNFEGRQGRGGRTHLVSPMMAAAAAIKGHFTDVR
                     DWDFK"
     gene            30644..>30825
                     /locus_tag="VFH96_08800"
     CDS             30644..>30825
                     /locus_tag="VFH96_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974107.1"
                     /translation="MKAFKKHTGLVAPLDRANVDTDQIIPKQFLKRIERTGFGEFLFY
                     DWRYTAGGALDPSFPL"
BASE COUNT         6619 a         8558 c         8924 g         6724 t
ORIGIN      
        1 acttttgccc acatcttgaa cactaatcgt tcgcgcgcgc tgccttgtcg ttccgtaatc
       61 tcgccgcgca ggtgtaacaa caacctcgga atatcgatct tcaccggaca aacttcccgg
      121 cacgcaccac acaagctcga cgcgtacggc agttgcgccg ctttttcgat tcccatcaac
      181 tgaggcgtga tcactgcacc gatcggaccg ggatacacag acccatacgc atgcccacca
      241 acctgctggt ataccggaca cgcattcaaa cacgcaccgc aacgaataca ggcgagcgat
      301 tgccgcgtca ccggatgggc caacattcgc gagcggccgt tatcgagcaa cacgatgtgc
      361 aactcgtccg gcccttcgtc agccggcgcg cgcttggttc cggtaatgaa tgattgataa
      421 gtagtcaacc gttgccccgt gcccgagcga ggcagcagct tgagaaacac atcgagatcg
      481 ttaaatcgtg gtatgacttt ctcgataccc atcaccgcga tgtgcaggcg cggcaaactc
      541 gtcgtcaaac gaatgttgcc ttcgttctcg acgatcacga tcgtgccggt ttcagcgatc
      601 gcgaagttca cgccgctgat accgacgtcg gcagcggcga aacgatcgcg cagctttgcg
      661 cgcgcggtgc tcgtcatttg atccacgttg tcggtaggcg gcatgccgag ctctgccgtg
      721 aagagcgcgc cgatctgatg gcgtgttttg tgaatcgcag gcgcgatgat gtgcgacggc
      781 ggctcgttgg cgagttgaat gatgtactcg ccgagatccg tttcgaccac ctgcataccg
      841 gcggcttcga gagcgtcgtt gagatgcgtc tcttctgtcg tcatgctttt gcttttgacg
      901 acgatacgcg ctgcgcgctg tgttgctagt ccgcagatga tttggttcgc ttccgcagcg
      961 tcgcgcgccc agtggacctt cgcgccgcga ctctccgcgt tgcgcacaaa ttcttcgaga
     1021 taacgatcga ggtgtagcaa gacctcgtct ttgatcgcgc gcgcctggct gcgcagatcc
     1081 tcccagttcg gcagacttgc ggcagcctct ttgcgccgtt cgccaaacag cgacgtggca
     1141 ttcttcaacg cgccacgaag ctgcacgtcc gcgagcgccg ccttcgcatt ctccttgaat
     1201 gtctcactgc tgagatgatt catttaacaa ttccgcgaga tgcatgactc gcactttcga
     1261 tcctttgcgt gagagccggc caccgatctg catcaaacaa ctcgcatcgc ccgaaacaac
     1321 tgcctgcaca cccgcgcgct cgatcgcgtc gatcttctgg tccaggatcg ccacggagat
     1381 ctcgggatac ttcaccgaga aagtgccgcc gaaaccacaa cacgaatccg catgttcgag
     1441 ctctacgaat tctgcgccac gcaccttgcg aatcagagct cgtggctctg atttgatatt
     1501 gagatcgcgc aagccgtgac aggcgtcgtg ccacgtcaag cggccgttcc acgatgcgcc
     1561 gacgtcttct ttcttgagca cgcgcaccag aaatgagctc agctcgtgcg tcttcgccgc
     1621 gatcgcgtgg gcgcgtgaca gccattggga gtcgccggca aacagttgcg agtaatggtg
     1681 gaccattgcc gtgcacgagc cggaaggact gacgatcgcg tcggctttgc tctcttcgaa
     1741 aatgctgata aagcgcttcg ccacgaggcg cgcttcgtcg agataaccag tattgaaagc
     1801 cggctgcgca cagcaagtct ggcgcttatc aaactcaacc tcacacccga cacgtcgcag
     1861 cacctcaaca ctgctcacgc cgatgctggt ccacatttga tcgacaagac acgttacgaa
     1921 aagcgaaacc ctcataatga aagcattagc cgcggatgaa cgcggatttc cgcggatctg
     1981 cgtttatccg cgaaaatccg cgttcaatgt aacgacacct ttcacatagt cgtcggtgtg
     2041 gatatcgctc gacaaagcct tctcgacgtc gctgaacgag gtgtaacgat gcgtgatcag
     2101 cggtgcgata tcaatgcgct gctgctcgat caggttcaat gcgcgcctgt agactgccgg
     2161 acgcccatcg ctctcaaagc caccggaagc gccgacagaa ctcacgagca ccggctcctt
     2221 gaacagtatg ctgctcaaca cgctgagatc ggttcctgca tgaccatgtc catacaacag
     2281 aactactgcc tgcttgcaga tcaatcccgg catcgaacga aagacctcgc ctgatcccga
     2341 cgcctcgatt aaatattcga cgccctttcc acccgtgtgc tcgtgcacca cctcgaccag
     2401 atctgcgccg gccggatcga ttgcctcatc agcgccgagc gacttcgcga gttgtcgctt
     2461 acgctcattc gggtcgctga cgaagagcag cccttcgtaa cgtagtacgt tgcgcaggta
     2521 ttgagtgaac aacaatccag caggtccagc gccgcatatt aacacgctcc gcacgcgacg
     2581 ctgctcgctt tgacgcgctt tcgctaccat gtcagacgaa tgaatgatgc aaccgagcgg
     2641 ctcgaccagc gccgctgtcg acgtttcgag cggcgcagtg atctttatcg cattcaccgc
     2701 cggcaccgcg atgaactcgg cgagaccgcc gggcaatccg gtgatgccgt gctcgcgata
     2761 aaactcacac tggtgactgt tgtctgtgcg gcagtattca cacaaaactt cgcgacgacg
     2821 gctgacgcaa ttcaagccct gatcgatcac gacgcgatcg ccttcacgca gatcgctgac
     2881 cgcacttccg acttgctcga cgacgccggt gatctcatgt ccgaggatct gcggctgcaa
     2941 gaccagcggg atcgcttggc cgtactcgtt tgtgttgtag tttgcgtgac ccgcgaagat
     3001 gtgaacgtcg gtgccgcaca gtcccactgc cgctacgcga atcagcactt cgtaaggcga
     3061 aattgcggga cgcgggatgt cccgcacctc cagacgttgc acgtcgcaca agacgctcgc
     3121 cttcatcgtg tgacccggta ctggtgcact catatgacgt tgccttaaaa gtcagggctg
     3181 attcataacg ttaaatcgct cgtgtcgagc cggccggcgt cgcgcagggc agcgaagcga
     3241 tccatcgttt cacgcatccc gatctccagc ggcgtcgaag gtagatcgtt gatgagacgt
     3301 ctgatcgccg agtcgtccat cgcggcggcg atcggaatcg gtggaccacc gaatgtgatc
     3361 aagtcgccgt tcgaagatgc gttgatgaac tttgcgatgc gctcgactgt gacggtttcg
     3421 ccgtgcaggt tgaagacgtg tgcgccttcg ggcatgttgt cggcacacgc aatgaatgta
     3481 gcggccgtat cggcaacgta ctgaaaatcc gtcgcgccac caaagcgaat gtgaaatggc
     3541 cggccgagca cggcagattt catggccttc gtcggatcgc tcgtgaggcc ggtgtcgcga
     3601 ttgacgccgt agacagtcag cggacgcaga ccgacgctgt tgataccgtg atcgagaaaa
     3661 tagacgcgcg cgttgccctc gttcgcgcgt ttgaagaccc cgtagtgcgt tgtcggttcg
     3721 caaagcgcgg actcgtcgag cgcaaggtcg tcgtcattca ttccataaac cgctgctgag
     3781 ctcgcgtaaa cgatgccgtt gaggccaatc tttctcgcag cttcgaagac gttaagtgtg
     3841 ccggcgacgt tcacgagtgc gccggtaacg ggatcggctt tgcaggttgg aacttgcagg
     3901 cctgccaggt ggatcacgcg cgccgctccc gagtcattta aagcagacaa cactgcggct
     3961 tgatcggtta tatcgccagt gatgaaacgg acgtgcgcga gatcgtcatc attgatgatc
     4021 gcgctgagcc gacgtgagtc gtcgctgcga tcgaacacca cagccgtatc gccacgctcg
     4081 gtgagcgcct tcacgatcca ggaaccaatg caaccctgcg cgccagtgat gaaataggtt
     4141 tcaggcatca gtcgagacga taaaactttg cggcgttgtt ggcgaatatc ttctcacgat
     4201 ctgcgtcgct tagagccttc aagatctcca aaaatccgtc gagcacgcga tcgtaggaag
     4261 ctgcgagtag acaaacggga tagtcgctgc caaacatcat gcgatccgtg ccgaacgact
     4321 cgagtgcgca gtcgacgtat ggacggagat ggtcaacgcg ccatgaatcc cagtttgctt
     4381 cagtgacgag accggaaagt ttgcaatgga tgtttcgata acgcgccaaa ggtttaattg
     4441 cgcgtgacca ttcatcgatc tcgttacgtg cgatcggcgg cttcgcgaga tgatcgatca
     4501 cgagcttcag atcgaggcag ctttcagcga cgaggggcac gtgtttcagg tgtcgagtgt
     4561 gcaccagcag atcgtagctg agcccgtgcg cggagagttt ttgtaagcct gaaaggatgc
     4621 gtggttgaac gagccagtcg tcattaggtt cgctctcgac gagatgtcgg acgcctttga
     4681 atttcgggtg cgcggggatt tcagggttgg ttagatcgac ccaaccgacg acacctgcga
     4741 tgaagtcatt cgattcagct agctcaagta gccaatgtga ttcggcgatg ctgttgctcg
     4801 cctggacgag gactgttttg gtgatgtgat tttgttgcag gatcggagcg agatctgcgg
     4861 ggaggtagtc gcgatagagc acacccagat cttctgacat ccacgggtag tcgaaacgac
     4921 ggggctgcca gaaatgttga tgcgcatcga tcattggaac gcggattata cggatgaaag
     4981 ccggatttac gcggatacgt cacgcggttt ttcggcgcgc ttgccttcat ggctttgctt
     5041 cggttcgctg tcgcactcga gggcgaaggt tgttacgagt tcgggagcgt ttttggggag
     5101 gccgacgagg cggatgcgga agtcgtcctg ctcaaatttt acgggggtgt tggtgctgtg
     5161 cattgtgacc gagttgacct tattgagaag attggcgatg acgacggttt cgccgggcca
     5221 gtagtgaacg tgcgcgtaga gcgtgttgcc gcggcgcgtg tagctcagat agttgccgcg
     5281 acgcggctgg cagttatcag aaagacgaat cgtgtcgccg tgtttcgcca tccagtcgcc
     5341 gacagttgat agaaccttta cggattcgag ggggatcgaa ccgtcctcag ttgggccaat
     5401 gttcaaaaga taatttccac catcacgcgc gcaggtgatc agattgcgaa tgaccgtggt
     5461 gggcgttttc cagttgcgat cggaaatgtg gtagccccag ctatcgttca aggtcatgca
     5521 cgactcccac ggccggttgg ccgatgcttc gattcgctgt tcgggcgtgt cgaagtcttc
     5581 ggggatcttc gagcgattgt tgatgatgat gtcaggctgg agcgtgcgca ccatggtgtt
     5641 catctcgact gattcccagc ctttcgcgtc gagcggccac ggcacgtcgt accagagaat
     5701 atcgaccttg ccatagttgg tcatcaactc gcgcacctgg ccgtgaatgt aagcgacgaa
     5761 gcgacggcgc gcggcttcat ccttcaggca atgcgcgccg tccggatggt gccagtccat
     5821 caacgagtaa tagaaaccaa atcgcaagcc ttcagcacgc gcagcctcga cgtactcttt
     5881 cacaagatcg cgaccgggcg ctttcttcgt ggcgcagtaa ttcgtgagct tcgagtcgaa
     5941 gttgcagaag ccttcgtgat gcttggtggt catcaccatg tatttcatgc ccgcgcgttt
     6001 cgccagtttg gcccaggcct cggcgcaatt cggtttcggc ttgaagttgt tggcatactg
     6061 ctcgtattcc ttcgctggaa tgccttcgtt ttccatcacc cactcgtgac ggcccagcgt
     6121 gctgtacaga ccccaatgaa tgaacatgcc gaactttgct tcatgccacc acttcatacg
     6181 gcgcgtgcga tcctcgatcg tggaggttgt cggcgcgctc acttcggcgg gcttgggatc
     6241 ctgagcggaa gcgagtttgg aagtggctag agcacctgca gctgcgccca tcagggcgag
     6301 gtagtttcgc ctgttcatta gttatctcct gggtttcgtt agcggtaacg gatagtacca
     6361 cagattgtcg cggatgaaag acggatttat aaaaattttt cccaacaggc gaggatcgtg
     6421 cggagctcat cttcttcctc gggcttgacg ttgacgagcg gcgggcggac tggaccacca
     6481 ctcagaccaa ccatatccat catcgctttc attgctgaaa cttcgtaacc attgcgacgt
     6541 gcgcgcagtg cgtagagggg aacgacgcag cggtccagca agtctctgag cgtttcggag
     6601 tcaccggtgt ctgcgagctc ccagagtttc aacgagagtt gaggcgcaac ggtcgcgatg
     6661 cttgaagtga atgttgttac accgatgctg aagtaagcgg caaccatgtc gtcacccgcg
     6721 ccgccgatcc agtgcaggcg atcgccaacg tgattcatga tcatctgcaa gcgacgaata
     6781 tcgccctgac catctttcca accgatcaaa gtcggtatcg cggtgagacg ctcgaccagc
     6841 gatggactga agctggccca gtcgcgcgtg tagatcaaca tcccaaggcg cgttgcttta
     6901 ccaatcgcgc ggtaatactc aaacaatccg tcgtcttcgg cttgcgggta gtaaggcggg
     6961 aaggcgagga tgccgtcggc gcccgccttc tctgccgcct gcgccatctc gaccgcaaga
     7021 cgttgcccga agccgacgcc cgcgatcacc ggcacacgat cctcgatcgc gagcgcagtc
     7081 agttcaacta cgcgcgtgta ttcagcaggt gtcaacgaat acaactcgcc ggtgccgccc
     7141 actacgacga tagcggcgat cggatgctcg agcagcttcg tgaggttctc gtgcagtccg
     7201 ggcagatcga gtgacagatc ttctttgaaa ggcgtgaccg gaaacgcgat cacgccggag
     7261 agtttcttgc ggagcgtgtt tggatgcatg attttagccg cggattttca cggatgaacg
     7321 cagatcagca accgtccgga gcgtgcgggc atgtttttga tctcgaacgt ttcggagtgt
     7381 atccctaaat cagcctcgct ccaaaaatcc gtgaggcggc agcgtcgcgg gagacgaatc
     7441 gaaatgtctt gcggagcgtc gttccaattc attagcgtga tcacttcgcg gtcctttaac
     7501 tgaactcttc ccacacgaaa tgagtcgtcg tcgaaacgcg cggcgacgcc tgtcggtggt
     7561 tgcagcttgc gcaacatcgc gagcttctcg gcgccgatct tcgtgagatc gtcgcccgac
     7621 aaaagcattc caccggacgc gtaaatcgca gtcgcatgaa actgaaactc gttgtccggc
     7681 agatcgcctg tcaacacgat cgcgtcggaa tcgttccacc acaaacggcc gttctgccag
     7741 ttacgactga ggttttggcg cgcggtcgtc tcaatgcgtt cccaggtgcg cttgatgtcg
     7801 ttcgagctgc gcgagccgtg aatcaatccg agcgacggcc agatcgggtg gttgcaaccg
     7861 agcacgaaac tattttccgc gccgcgcagt atcgcgcgca tcccggagcg ataagcctcg
     7921 atacgcgtag cgcgttggtc gtgaaagcgg ccgccgtgga tcgcgcccca gaagtttgcg
     7981 tcaagcttga agtaagtgca gccccactcg cgcttcatcg tgcgaaaaaa cttttcaaag
     8041 tgctgctgga cttccgggtg cgtgccgtcg agcacgtacc atggaccacg acgccagccg
     8101 ccgaacgtga cgcgatcgga ttgtaacggc gcgccgtctg cgtcctttac aaaccaatcc
     8161 ggatgctgtt ggaacacttg cgacttttct tcagcgacaa acggtgcgac ccagatcgcg
     8221 ggctcgaagc cgcgcgcgcg aatctccttc aacacacctt gcacgtttcc gccgaacgcc
     8281 gcgccggtct cgagccagtc gcccatcgcc ggctggtagc cgtcgtcgat ctgcacgtaa
     8341 cgcaaaccgg gcgtgtgctt cgcgatgaag tcgagattgt cgcggacttg ttgcgcagtc
     8401 acgcgtgggc cgaagcagta ccacgagcac cagccagtcg gcggcgatgg aaattttagt
     8461 ggcgggtgat tggcgctgag ctgtgtcgcg agctgatcga gtaactgttc gcgatcgttg
     8521 ccggatttat aggtgaagga ttcgagttgc cattcctcac cgggacgaag ctcgcggcct
     8581 tcaatgtcga gtacgacctg gagcgatggg tagttgagat aaaactggcc cataaagcgg
     8641 cgacacgaag tgaatgcaaa caggtgatga tcgtgcgcgg cgggcgagag catcatcatg
     8701 ccgtagcaag ctttcgtgtt gggcggcgcg ggaagtttgt agtgtttggc gtcggtgtag
     8761 ttgccgaggt ctgctggttg gcccaaactg ccgccggttt gcgagagcat ctggaagcct
     8821 tcgccgtaga gcttcgtgcc tggtgggaat gatagtttga gatcgaagag aacgatctct
     8881 ttgatcttca ccgcgtcgcg tccgtgattt ttgatccggg ttcggccacg atcgatcttg
     8941 attcgcgaaa catccgtgcc gtcgactaac ggaatgactt tactcggtgc attttgaaga
     9001 cgaggaaagg ctgcgattat cggagagacg atagccagtt gaaggatttg tcttcgagag
     9061 atcatttttt acatgattac cggatttaca tgatttacaa gatatcttga aaaatcctgt
     9121 aatcctgtaa tcctgtctaa aactacctcg tcagcttcgc gtccacccag tcaacataaa
     9181 ctcttccacc ttcaccaacc cttcgcacac gcaacatcaa ccgcttcaca ttctttacat
     9241 cgatctttag tgattgcgac gtatcactct tccacaattc ctgcccgtcc ccaattacag
     9301 aaaactccac cttcgccttg ctgttattga cctcatgatc aatccgtacc agcgcgacga
     9361 agctgtcgta cgtcccattc aactcaaact caatctcgga attcgtcggc atgctgatgc
     9421 tgttgacatt gcgattcagc tcaggctgcc gccaacctgg tgtcagacgc accggctcga
     9481 gatcggacag aaagatctcc ggcggctgga tctttttgag cgtgaacgat agctgtgcct
     9541 tcttctcact cagcgtccca tcctgccaga cagttcgcac ttcggctgtg tagttagtgt
     9601 tgggatcgag gccgcgcagc gcgaacgttt gcgaggttgt gtaaccgagg accgttccgt
     9661 tcagtgagac ctgataagcg aatgtcggct gcgccgggat cacccaacgc aggttggcac
     9721 catcgacacc gtaacgctcg gcccaaacgc gctgcggctc tttcaccggg aagtgataag
     9781 ccggggctgg ttcgaagact acttcccaac gcacttctgt ggttttggcc ggtgtgatct
     9841 ccacttcagc ccaaccctgg tgattcacta tgcggaccgg cagtccactc gcggtcgcgc
     9901 tttttatacg gaagttctta ccgcgaggaa aagcaaaaca cagattgtac ggatcgttgc
     9961 ggattagttt gcttttgccg gtgtatcgat ttccgctgat gctttgcgag attagatcga
    10021 cccatccttg cgtgatatga cgactcgtcg agacgagttg gatctgatcc gttgccggga
    10081 cgagcgataa cacacgcgtg cttgcaggag acaattcgag agagaggcct ttctcaaacg
    10141 cgccgagata ttccttgttc caaaagtcaa aaacgtgcat gggccgatct tccagtaatc
    10201 ccagatcttt ccacgagagg taagaagagc tcggcttgct ctcgtcgaag ttgaagacgc
    10261 cgacaacatc gtaggcacga ccgaggtgat tgatcttcag gtcccagatg cgcttgttac
    10321 gatccgaaga aaacagatcg agcggacgaa tatccaccgc cggatacacg cgcttgatca
    10381 gatccacgcg atccgccgac agatcgacga gccgatcgct catcaacgcc gcctggcccg
    10441 tcaatccctg caacgtggcc cacgcgcgcg cctgctcgat cggcagcggc gcccgcacga
    10501 tgaacacgtc cggatccgtg taccacgcaa cgttgtgcaa aaagtaatac tgcatcgtcg
    10561 cgcgtatcgc gaatttaaat ccatcccagt ttggcaacac atcggcgccg atacgcgaac
    10621 cgttcatcag cccgacgcct tccagcggca cgacccagca gccgagcaga taactgcctg
    10681 gtccaatgcc cgcacgaatg cttttcaacg tctcgcgata gagcgcgtct gtgtcgtctg
    10741 cagggttctt catgaagctc ttcttgttgc gatactcgcg cacgacgatc ggctggccat
    10801 cgatcttgaa atactcgtaa ccccagctcg aaagcttcgc aaacaagtct ttcaaatact
    10861 gctgcgactc aggcgctgaa ggatcgacga gaaacgttcc ttcccacgtg ttcgacgccg
    10921 tcgtgccgtc aggcttcatc atgaaaacac cggcgtgatt cttgacgagt gcttcgttgc
    10981 tctggccgtg cggggcgagc cagatgccgg gtttcagacc aagagatttg atgtacgcgg
    11041 cgagaccatc catgccgctg ggaaagcgtt tgttgatggt ggaccagtcg cggttttcac
    11101 cgaggccgtg gccggtgccc tgccagccat cgtcgatctg aacgtactgc acgccgaagt
    11161 ctttgaggtt ctccgcgatc cacttcgcat tcagcttgat ctcgtcttcg ttgatctcct
    11221 ggtagaagaa gtaccaggaa caccaacccg aaggcggcag tgggaagcgc gttttatcga
    11281 tcggttggaa gtatttgatt ttgagcgtgt cgcggaagta gttcttgata acggcgccgt
    11341 cgttcaggcg caactcatct ttcgttggat taaaagtgcc gtcggcgatc ggtgttgaga
    11401 cttcgccgag cgtcatcacc atcagatctt tgtttgtgcc gtcatgcaca cgcgccggcc
    11461 acgtttgtcc aaaaaccgat ccagccgcga aaaggcacaa aaagcacgaa aggataaatt
    11521 tgatctcctt catgtctttg tgccttttgc ctttttgtgg ctaaaaactg aacttgatcg
    11581 cagcctgcat cacacgtggc gtgcgagcag cgatgggagc gccgaagcgc gtgttggtct
    11641 gattgcctgc cgcatcaaac accaaccccg catcaatgtc cctgaagttt gtgtgattga
    11701 aaacgttgta aacctcccaa cgcagttgca gcgcacgact ctcacccatc ggaatattct
    11761 tgaacagcgc gatgtcgttg ttgaagatcg acggcatgcg caacgaattt cgcggcgtgt
    11821 tgccgatgtc gccaagtcgc gtcggcctcg caaagcaggt aacgttcacg acgtacggcg
    11881 tacccgtggg atcagaacca ctaatgttgc gcatcggatc gcaaatcgta ttcggccgcg
    11941 cgttgtcctg gccaccggtt atatcggtga cgccgccgtt gtaagtcacc gagatgttct
    12001 tcggcttgcc gctcgcataa gaagtagtgc cggtaagctg ccattgatcg aagatcgctt
    12061 ttacgaatcc gttgttgaac ttacggctca gattcggaat gtcgtagatg tagttgatcg
    12121 tcaggatatg cgtctgatcg aaatcggacg gcgcatagtt gaagtccttg taaggacgat
    12181 taaagaacac atccgacgaa tcatcgttcg catagtcaaa cgatttcgaa taggtgtaag
    12241 ccaaaccgta ctgaaaacct ttcgtgtaac ggcgcgtgat ctgcgcttga agtgagttgt
    12301 agtttgaagt gccggcccag gtgaccatgg tgatgtcggc ataaccgcga tacgggcgca
    12361 ggaagtcgtt gccgattcgt tgaccagtca ccggatcgat gttgttcgtg ccgagccggg
    12421 cgccatcagg cacgccgttg atgttgatgc gctggcccag gtgacgcgca aacgatccga
    12481 cataactgac ttccatcacc gtctggaaac cgatgtcctg ctgaattcca aaggtgaagt
    12541 tgtagatcgc cggcgtgtgt gatttctctt ccaccgcgtt cagcgcagac ggtctcgtca
    12601 aggcggtgcc ggtcaggctg gcgaggttgt tgatgttgcc gaagtcgatg ctgaacgtcc
    12661 ggttggcagg ttgattgtta accaggttgc cgccggttgt gccgccgccc acacgcggcg
    12721 cgtgataaac gccaccctga aaacgaagca cagatttacc cgaaccaaaa acgtcccacg
    12781 cgagaccaaa acgcggctcg tagtcgatcg ctttcgtgtt tctgtaaccg ggcgtcgtct
    12841 ttggatcgat gccgagagcg agaccattgt tggcgtcgcc gctgttcggc acaatcgcgc
    12901 cgacaagacc cgcgttcgca ccaaccagcg ggatcagctc gcccgtgacc gggttgcgcg
    12961 cccggcgttg cgcggccggg caagtcgccg gaggagtaaa agtcactgcg cacgcactct
    13021 catacaacgc taccgcgcgc gaccggttga ataatccctg gacaaagttg gaaccctgcc
    13081 catcgatctg ttcgaagggc gagtgatagc caaaacgcag gccatagttg agcgtaagac
    13141 gccggtggat cttccactgg tctgccgcgt accactgcac ctgcgtcagt ctcagattcg
    13201 tgaacgggcg cgcggttgat tcctggtagt ttctgaagtt gccgatcaag gcattcgcgt
    13261 aagcgtaact ggtgttgccg agtgcggccg tgtagttgct gtcgttgccg gcgaagttga
    13321 acacgcccga ccattgtcca cccggtgctt cgccgttgag cagtcgctcg aagtacacac
    13381 caaacttcaa tgcatgatcg ccgtgcgtga tcgagaggtt gtcggcgaat gacggtttga
    13441 tgtagtcgtt tccggtttca ccccagcgat cgagccagtt gatattggcg acaccgttag
    13501 ttggaccacg aacaccgccc cagtttgtaa ctctgggaat cgtgccaagg tgattgtttt
    13561 gtgggaagag ctgcggcgcg gtgtagttca aagcccctcg ctgcacgcgc tcgatctcac
    13621 cgtcaccggg aacaaagcct tcggaatcgt gacgcatgcc gaagttgaat tcgttgacga
    13681 agctggcgtt gatgacgcgt acccagttag cggaccagcc atcgtctttg tagaggtagt
    13741 gtgaattgat cccccaacga ttgttgtcat tgccgggcca gccggaagtg ccggtgccga
    13801 gattgtcaga cgtccaccat tgatacttcc aataaatcgt gtcgttgttt gacggtttga
    13861 tgtcgaagcg aaggacctgg ctgcgcttgg gaacgtcctg cgggctttgc gagacgtagt
    13921 tgaaagcaga gccgccggag ccaccgaacg tattcggcaa cgggaaatag ttgagcaacg
    13981 cgaatccact cgcgttgaag cgcgtctgtg gaatgatgtt gagtccgagc gggttcgacg
    14041 ccgtggcgcg cgacggatcg cgaaagcagc cgctcgtgtc agatgcagcg caaccgcccg
    14101 gcagcagtgg atctttcaca aacactttct gattcgccga gttgatcgac tgggagaagt
    14161 cgccgttgcg ctcgagcgcc gtcggcatcg tcacgaagcg tgggtcctgt ggcgtgatct
    14221 ggtgcggctt ctcgtaggcg aagaagaaga acgctttgtc tttgagccag aagttgccgc
    14281 cttcaccgaa cttcggcagc cacattggac caccgatatt gccgcccgga tagagatgac
    14341 gataaagcgg tttcttcaaa cccgctttgt tgttgaaaaa tggcgtggcg ttcagcgatt
    14401 cgttgcgcag gaagtagtaa accgatccag tgaaatcttt gccgccgcct ttcgaaacga
    14461 tgttgatgat cgcgccgccg ttgttgccat attcagcgcc gtaattgtta cgcagcactt
    14521 tcacttcggc gacggcgtcc tggttgatcg tcatgctcag cttgttggag ccgctaggtt
    14581 cgccggcaaa caagccatcg atagttggca cagtggagcg accacgctga cccgagatgt
    14641 taggcaggtc ggttccgaag ccgtcgcccg caccttcgat gtcgtcgttg tttgaagtac
    14701 cggggaggag gcgcaggagc gaagtaacgt cacggccttt ggtcgagatc aggttgatct
    14761 gatcggcggt gaggcgcgca gtcaggtcac tgctttcacg ttcgacaatc gtgccctcgc
    14821 tcatgacgct gactgtttca ctcacctgtc cgggctggag tttgagatcg ccgagcgcga
    14881 ggttttcatt agcgctgagg atcacgccgc gctgttcgag tgtttgaaaa ccgacgcgtt
    14941 caattttcag agcgtaggcg cctggctgga gagcggcaaa attaaagcgg ccctcactgt
    15001 cagaagtgga gccgcgtgct tgtccggtct tctcgctggt cagcgtgacg gtggcgccgg
    15061 ggatcattcc gcccgtctgg tccatgactg cgcctgagat tgagccggta atcgtttgcg
    15121 cggttgcgac tgtgccgaac gcgagcgcga cgatgatgac cgccaggatt gctgcattac
    15181 aacgcagaaa actgaacatg ggactcctcc ttcgaaattg gcaaatcctt cggaaatgac
    15241 tgctaattca aattgtctct agttgggaca atttgaataa aaagacgagg ggactaccca
    15301 aatcaaaatt gcctactggt tttccggtta ttgaaacaga accgagcgcc gtagcgccgg
    15361 gttaagtttc cttatcacga ctggtgaaag agttagaccc ggcgctaccg cgctcggttc
    15421 tgttttgcgc ttcttccagc gcaaaagctt tttgggctcg aacaatgtgc tcgcgcatcg
    15481 cctcgcgcgc ttcttccgga ttgcgcgcgc ggatggcgcg atagactcga cgatggagct
    15541 cgagtgcctc gttgaagtcg tgggcgcgct cgatcgtctc gcggcgacgc tcgtaaagga
    15601 ttgccgagac catctcgacg agtgtggcca gcgttgggtt gtttgacgcg gctgctaccg
    15661 cgcgatgaaa ggcgacgtcg tgcacgagaa actgctgcgg atctttcaag gccgcaaaca
    15721 tgtcggcaat ctgttccgac atcgtcgcga gttgctctcc ggtggcgcgt tccgcagcca
    15781 gtccggcggc gccgacttcc agcaccgacc gcgtttcaaa catgtgctcg aaactaaaac
    15841 cgtgaagctg agcgagtagg cgcaggggtt cactgtcgag agctggagga ccatcgcaga
    15901 tgaacgtgcc tgcgccctgt cgcgactgca ggacgcccat cgagatcaaa gagctcaggc
    15961 ccgcgcgcaa agcgggacgg ctgattccca gttgttttga cagctcacgc tcgggcggca
    16021 ggcgttcgcc gggctgcaaa tcctggcgct tgatcagctc gtagacgcgc tcgacgacct
    16081 gttctgtcgt cggcgcggaa cgcagcggct gaacaacttt caacttcgga accgtggaat
    16141 tggtcataac aattttgagc cgggcgcgaa acttataata ccaattcaga ttggcgtcaa
    16201 gtttttatcc acagattaca cagataaatt gcttaatctg cggaatctgt ggatggctat
    16261 cgtggttgcc aaagcgtgcg gctgctgccg ttgagcgttt tgttcatgaa cttctcgttc
    16321 atgatcagaa atgcaccgag accgtggaag tcgtttgtgt tgcgcttgcg tgcatagtag
    16381 taagcgaggt ccgcgacgtt tgtgccttcg cagatgtcaa tgaggtttgt ttgaccgtct
    16441 ttgtcgagcg agatcttcgt gagcacgccg gcgtaacctc gattcacccc agccgcatac
    16501 ttcttctcga catagccgcg atcgacggcc atcgcagtca cgtacgtgta catgctggag
    16561 ctcgacgtct cgaaccaatt cgcaggatca ttgcctttat caacaatctg ataccagagc
    16621 ccagtttttt catcctgata acgcgcccac gctttcacga gcaggctgag atgaccaatc
    16681 agcaacgggc gctgcggatg attgcgcggc aacagctcga gcacctcgat gagcgtcatt
    16741 ccaaaccagc ccatcgcacg gcaccacttc tccgcggaat gcttcgtttc tttgtcggcc
    16801 catgcggatt gcccgctctc gtcgtaagcg tgaaacatca ggccggtgta aatatcgttc
    16861 aaatggagcc agtaggtcag taattgaaga acggcctcat catttgcgta tttagcgtcg
    16921 ccaaacatgt ttccgtagcg caccagaaac ggcatcgaca tgaacacgcc gtcgccccac
    16981 aactgccact cgcggctctt actcgtcgcg tgccagaagc cgtcgtcttt agtgcgcggg
    17041 taactgtcga agcgcttgcg aatgcggtcg gcggcaagtt tgtatttttg ctcttttgtc
    17101 tcctggtaaa ggagtagaag gagattgccg ggcagcatgc tgtcgaggtt gtcaaacttg
    17161 atctcctgca cttcgccctg tgcgttcgcg ctgcggacaa cgccctgatc gtccacgtgc
    17221 atgtctaccc agtccttgat gtacttgaga taacgaggat cgtgcgtgcg tttgtagacg
    17281 agatactggc cgtagaggta aagcgattta gcgtaacccc acgaaccgag atctttcgcg
    17341 gtgggatagc gcttcatggt cgattcgacc gtggcgacgg accaatctgt tcttggttgt
    17401 gcgatattgg agaaagggat caataacaac aacgcgatta cagcaatgag cttcattggt
    17461 tgtcctcgtt tgtttagaca ggatttacag gatttgcagg attgaatctt gtaaattctg
    17521 aaaatcctga aatcctgtgc taatttatgc ggaccgatta cagtccaaaa tcaatctgga
    17581 aatggtttga ccaataacac aaaccgcggg agagaagcaa tgaccaagtc tcgtgataat
    17641 cgtgacgacc acgatcctgg caggcggaag tttctgaaag gcgtcggtat cgccggcgcg
    17701 ggcgctgcta tcgccgacca cttgtgggcc gaagcagaag cggcagaacc tcctgccacg
    17761 ccggaatcgc tttcgggatc agtaaaagtc gttctcgacg tgaacggaca aaagcgtgag
    17821 gcgcaggttg agccgcgcac gactttgctc aacacgctcc gcaatcatct cgatcccgcg
    17881 gtcactggtc caaaactcgt ttgcgacgtg ggaacgtgtg gcgcgtgcac cgtcctgctc
    17941 gacggcaagc ctgtttactc gtgccttgtt ctgggcgtcg atgccacaaa cagaaagatt
    18001 acaactgttg agggtttagg aacgccggag aatcctaacg ccgtgcagtc agcgttcgtc
    18061 gagaacgacg ctttgatgtg cggcttctgc acgcccggct tcgtcaccac gatctccgcg
    18121 tatctgaaga agaacccgaa tccatctctc gcggaagttc gcgaagcgtg caagggcaac
    18181 ttctgccggt gcggaacata tccgcgtgtt ttcgaagcgg ccctcgcggc ggctaaagga
    18241 tctcaggctt aacaggaacc actcatgcaa caacagcaac cacaagcgca accgtcacca
    18301 tcgcccaaac cgaagaaaaa gatccgcgtg ccgcgcgtcg tcaacggcgt cgagcagatg
    18361 gtcgagatcg aagtcgacgc agacaccgga ccaggctggg gaccaaacga taaacacaag
    18421 ctactcaatc aacgttacac gcgtgtcgat ggtccactga aagccaccgg tgtcgcgaaa
    18481 tacacctacg atcagcgtgt gccaggcatg ctttacgcgc gcatacttcg cagcccacac
    18541 gcgcacgcgc gcgtcacgaa gctcgacact gacgccgcgt cgaagattcc gggggtgaaa
    18601 gccatcattc ccgccccgct aactgaagtt cgcttcgcgg gcgcgccggt tgccgcagtc
    18661 gccgccacga cgcccgagat cgcgggcgac gcactgcgcg cgatcaaagt cacttacgaa
    18721 gtgttgccgc acgtggtcca cgcgcaccac gcgattcgac ctgacgcgcc taaagttctc
    18781 gccgatgaag agaatctcat ggaaagaggg aagaatggcg acgctcaaaa agccgaagct
    18841 gcatttgcga ctgcagacgc gatcgtcgag ggcgaataca tcacctcgcg cgttcatcac
    18901 gtgtgtctcg aaacgcacgg catggtcgtc gattatcgcg gcggcgactc cgccaccatc
    18961 tacgcgtcaa cgcagggaac attcacgatc cacgaggacg cagccaaaga attgggactc
    19021 gccagcaacg cggtgacgac cacggtcgag catatgggcg gcggcttcgg ctccaagttc
    19081 ggactcggac tcgaaggcat gctcgcgtgc cgtctctcga aacaaacaaa ggcgccggtg
    19141 aagctgatgt tcacgcgtta cgacgagttt gtgatggcag gcaatcgctc gggatcgtgg
    19201 cagaagttga aagcgggcgt gaagaacgac ggcacgatcg tcgcactaca ggcgcgccag
    19261 tatcgactcg gcggtctcgg ccagggctcg caggccggtc agccgttcat ctatcgcatg
    19321 ggcgacacgt atcgtgaggt ttatgcgctg cacacaaacg aggattcgtc ggttgcgatg
    19381 cgggcgccgg gttttccgca agcgtcgttc gccgtggaga ccctcgtcga cgagctcgct
    19441 tacaagatca agatggatcc ggtcgagttt cgcaagaaga acctgcgcga cgaggtttat
    19501 caccggcagt tggatcgcgg cgctcgcgag attggctggt ccaggagaaa tccggttgca
    19561 ggtggaaatg ccggtccatt gaagcgcggg atgggttgcg ctgtgggcac gtggggcggc
    19621 ggtggaaaca accagtgcaa agttgatgtg acgatcgcgc gcgacggatc ggtactcgtc
    19681 gccgtcggca cgcaggatct gggcaccggc acacgcacgt acatgcgagc aatcgtcgcc
    19741 gaggagctcg gtctcgggat caaagacgtg aaagagcgca tcggcaattc gaaactcggc
    19801 gcggcaaatc cgtctggtgg ctcgacgacc gcgccgtcgt tgtcgccgtc agtgaaagac
    19861 gccgcgtaca aagcgcgcct gttgatggct gagcgcgtcg cgccgctgct tggcaatccg
    19921 aaacctgaag acgtggtctt cgctgatgcc aaggtttcag caaatggcaa gacgttgtcg
    19981 tggcagcagg cggtggcggc gctccccgcg gcgggcatca cgtcgcatgg tgagtggcgc
    20041 gtggacttgc aggcgcgtgg cattcatggc gtgtgtttcg ccgaagtgga agtggatgtt
    20101 gaaaccggtc acgtcaaacc gatcaagatg gtccacattc aggatggcgg cttgccgttg
    20161 aaccgtttga cgatggagag ccagatcaac ggcggcatga tccagtcgct cggcatggcg
    20221 ctttgggagg gccgcgtgat ggacgcgcag ctcggcatga tgctcaatcc cggcatgggc
    20281 gattacaagt tgccgggcag tctcgaaatg ccggagatgg tgccgctcat cgacgatgaa
    20341 gataagcgcg aggccgtgat cgggatcgcg gaaggttgca tcattccggc gctcggcgcg
    20401 cttgcgaatg cggttttcaa tgcgacgggt gtgcgcgtgc gcgagacgcc gatcacgcct
    20461 gacaagattt tgatggggct aatgaattcg actgccctga gccgcggata atacggattg
    20521 aaagaggata ttcacagatg aaatcgtttg aatggacgaa tccggccagt gttggtgatg
    20581 cagtgaagat gctcgctgtc tcgacgcctg gcgatcttga tgacgcgccg cggccgatcg
    20641 cgggtgggca ggatctgctg acgacgatga aagattacac gtcgcgtccg gtgcgcgtcg
    20701 tgaatcttaa aaacattcgc ggactggata agatcacact taacgcgcgc gggctcacga
    20761 tcggcgcgct cgttacgttg agtgagctcg aggagcacgc gggtgtgcgc aagaattttc
    20821 cagggcttgc tgaagcggcg cactcgatcg cgacaccgca gattcggaat ctcggcacgg
    20881 tcggtggaaa tctctgccag cgtccgcgtt gctggtactt ccggctcgaa gaagtcaatt
    20941 gtttgaagaa gggcggctcg gagtgttatg cggcgaatgg cgagaacaag tacaacgcga
    21001 tcatcggtgg tgggccatcg tacatcgtgc atccgtcgga tctcgcgccg atgttgctgg
    21061 cgctcggtgc gtccgtaacg gttgttggtg cgaaaggaaa gcgttcgatt ccgttggata
    21121 agtttttcac gttgccgtcg gagggaaata ttcgacgtga gaacgttttg aagaacgaag
    21181 agatcattac ggagatcgtc gttccgtcgt cgccggtggc tgcgcgttcg acgtacttga
    21241 aattcaaaga gcgcgagtcg ctcgacttcg cactcgcgtc agccgcggtt gcggtgcagc
    21301 tcggtgcgaa tcgagaagtg aaggatgcgc gaatcgtgct gggtggcgtg gcgccaatac
    21361 cgtggcgcgt tccggcagcg gagaagtttg tcattggaaa gagtctgaac ccggaagtgc
    21421 tcgccgagac cgcgaagatc gcgctcgccg aggcacgccc gcttgagaag aacgcgtata
    21481 aagtcccgct cgcccaaacg ctcgtccgcc gcgcgttggc caaagccgga actgtttaag
    21541 tttggagaat cgttatggca aacgaattag tcgaaccaat gcaatcagca ttctgcggat
    21601 cgttgcgctc gaagaagtac tacatgctcg atcgactcgc caccagcgcc gacgagtatc
    21661 tcgacgcggc caaccacgtt tggtgctgtg aaacgcagga agtgatcggg cccgacaacg
    21721 ctcgcgtcga tccggagcgc tgcggtcccg ggcgcgcttg ctacacatcc gcactcaaat
    21781 ccgtataatg aaattcggag gtatcgaaat gcgaaaagga ttattggcag tcgcagcgat
    21841 gctcgttgca gtgagcgcgc ttgtattcag ctcgcaagcc gagaatgtca gtcttcgtgg
    21901 ggattacgtt gaggtgcgca ccgcaagcgt gtttgccggt gcgtgtcact acaacggcga
    21961 agtagtcacc accggacgcg atgcgatgat ggcgtggaac gtgacctccg gaaaatggca
    22021 gggcgtcgat ctcagcggcg tgcgcgtgct ggcgatcgtt agctccgacg cgaacctcag
    22081 tgaaagcaat gcggcgcgac agtcggagat catcatcgac tcgaatgcct cgcgcacaca
    22141 agcgctcgca atggtcaacg cgctgaaaga aaaatacgct gcgtcgctcg gcaacgttgt
    22201 cgaagtacga agcgcgccga tcagcttcgc gcgcaatggt cgtgagtatg cggtcgtgac
    22261 gaacgaagcg gcgattaacg tcgaagcgat gccaaacgat ctctgctgca agatgccgaa
    22321 cctggtctgg tacgcaccac tcgtcggcct tgagaatcgc aaggtaggtt acacctcgaa
    22381 ggcgctctat tccggtaaag ttgtcggtga gccgtggtcg cgatcgggtg agaacagcgc
    22441 attctacggc acattttcac tttaaccaca gattacgcag attacgcaga ttaatgctta
    22501 tctgtgtaat ctgtggataa aaatgccgtg ggaagggcgt ctgcggaaac actcaaaacg
    22561 agctgccctt tactctccac actaatttgc gcccgcccgt tgtagagctc gactttgcga
    22621 gaagtgcttg acgtaccgag gttgtcgatc aggcgcccgt cacctgttat tccaaatctc
    22681 acaatgtttc gtgcatcaag acataacact cccttcgcat ccacaagctg agcttcaact
    22741 atcgagttag aaacgttcag cactagcttc gccggcttat cccacttctc cgtctgatac
    22801 acgaaccgga tctcatcctc tgcgtttttt cccaccgcgc gcacaacatt ctcacccgct
    22861 ttgaacttca ccagccagcg caaccccgcc gccggaaagt cctggctatt ccgcttctta
    22921 acacccgccg aaacaccatt cacaaacaac tcaaccttcc cgcagtttga ataaaccttc
    22981 accagcttcg cttcgtcagg cgcgccccag cgcaccggcc acgtgtgccc gtagatatgg
    23041 accatcggct tctcagccca gtacgactga aacacgaaat acccttcctt caacgtgaga
    23101 tcgcgctcga cgagtccctt ctgattaacg cgcggcaccg gattgccggg tcgcaacgtc
    23161 gtcgcgaagt ctttgaagat ccactgcgcg ctgcccgtca gctcaggcat cgtctcctgc
    23221 tctttcaaat gccagtcaaa cagatcgcag atgtacgtct ccgaccaatc gccttcttgc
    23281 ggcgcacgcg cgaaaacctc gctcgcttcg gaatgacggc gcgcgtgact gtcgccgccc
    23341 cactccatgt gcagaaagtg atcgactttc ttgatctccg tctgcacacg cgtcttgtat
    23401 tcggtgtatt tgccctgata ccagccagac cagattgacg gcgagtaaac atccaccacg
    23461 tctttgcaga aatcgcaacg ccggatcgcc gtcttgcgcg acggatcgag cgtgttgagc
    23521 tcgatcataa aggcgcggat cttgtcttta tcaaacgatt cgaaatcgcc gggccagtcg
    23581 ttctcgttgc cgagtcccca gataatgacc gagggatgat tgcgatgctg atcgatcatg
    23641 ttgcgcaaca tgcgccgcgc ctgttcctgg tagcgctcgc caccgagtcc gccgcgacac
    23701 cacggaattt cttcccacac gagcaggccc agcttgtcgc acagatcgag aatcgtgcgt
    23761 gactgttgaa agtgtccgag gcggatgaag tttgcgccga ggtctttgat caacttcatc
    23821 tctttgcgca tcagatcctc ggtcatggcc gcaccgacgc cggcgtgatc ttcgtgacgc
    23881 tgcgtgccgc gaatcaacag tcgctcgccg ttgagtttga atggtccgtt tttgacgaac
    23941 tcaaacgagc gtacgccaaa ctgcgcggcg atttttgata ttcccggctc atttgcgaca
    24001 gttacaacac atagatagag cgaaggcttt cctggcgacc acagcgacgg cgagtcgatc
    24061 acaaacgtgg tcagatcgag tgtgcctgtc cacgggttca gcttggatgt tttggaatga
    24121 accacctcgt tatccggacc cttaactttg acctccagct cgacctcgtc cttgatgccg
    24181 gtaggattgt aaagccgggc gcgaacagtg acccttgctt ttccgtttgg cagcaactcc
    24241 ggcacgacgt gcacacgctc gatggaaagt gccggcacgt agaccaggtt cacgtaacgg
    24301 tatagcccgc cgtatagatt gaagtcgctt aaattcgacg gaatcatctc caggtcgcgc
    24361 gagttgtcgc acattacggc gacgggcacc gcggcctctc ccaaaatctt tgggcgcgcg
    24421 ttcacctggt cagtgatatc gatcgtgaat tcatcgtagc caccgacgtg cgagccaacc
    24481 ttgtcggtat gaatgaacac ctcggtcttt tgtcccgcgc cttcaaagtg cagcagcgtg
    24541 cgacccgagg gatacggatt cttaatcgcc agcctcgttc gataccagcc gggaccctgg
    24601 taatacggaa cgtccggatc gacagcatca aaagcgttga agcaatgtgg tacttccact
    24661 tttgtccaaa cgttcgcgtc gtcattagct ttccgccacg cttcccatgg accaccgaga
    24721 ccgccgcgcc ggtattccca ctccctcaat cgctctgaga gtaggtatgg cgcgctggtc
    24781 tcgccgaggt aagaagcagg cttgccgagc agcgtgccgg ctaccaatgc tgttgatgcg
    24841 gtttgtaaaa aagctcgtct actgatgggc ttcatttgtt aaccgcggat tatacggata
    24901 aaaatccgga tttcatctgt taaatccgtg gttaaataag gactatgaac gcatacctgt
    24961 caattgcatt tattcttctc ttccaaaatg gtcacatcga tctccgcaag accgcactca
    25021 aatcaacaac tccatcagac gagatcgtcc gtctcgacat caatcacgac ggcaagcctg
    25081 acattctcga gcgctggtgg aacggcaagc gcgtccgctg gctcgatgaa aacggcgaca
    25141 tgctcccgac cgacacgcgc ggcgatcagg tcggcgacgt gatgcagatc gacaaaaacg
    25201 gcgacggcat ttacgacagc gaaacagaca tcaacatcaa gtgggctgac aacgacggcg
    25261 acggacgcgc cgatctgcag gccttcgtca cccaggggcg cgcgtggtcc aacggcaact
    25321 tcgatcccgc tgagtcgcac tggatggtct tcatcgacgt cgagaaagat ggcgtgctcg
    25381 gctgggtcga ctggacaaag tacgatttcg gtaacgacaa ctggggctac accggcacga
    25441 cagactggct tcccgactac aacggcaacg ctatcttttt gaaagttcat cgcccgccgc
    25501 agtcgctgcc cgacccgcgt cttaattggg aaaacccttt tgctttcttt gacttcgatg
    25561 gcgatggggc gagcgagatg gcgatccgct ggctcgatcc ggtgccgccg ctcgacaaag
    25621 atgtgaccaa actctcaggc gtgctcagcg aagcgtttgc gacgtttgat ctcgacaacg
    25681 attcgactaa aggtaacgag actgattacg acatgtcgct gcgtggcgcc ggtggtccag
    25741 gcgttccgta ccggcagttt gtgcacaagt atccggcgct caaaggcaat ccgaaattcg
    25801 acggttgctt tcagtggaac aactggcggc agatcgatga gctgatgtac atgccgcacg
    25861 agaagagtta cgacgagttt ttcaacgcgc gttgggccac gatgtatttc gtgttcgacg
    25921 aggacgacga cgatcatcgc tgggaacgtg ttgagatgta ttacccgatg cacggttttg
    25981 gtggtccaaa ggatatcgat ctgtattcga cgaagaagtg gcgtcgcggg aactatgcgg
    26041 agctggcgat ggccgggcct aacgagaagc ccgggctttc gggacatccg caggctgatt
    26101 cgctcggcga tcgtggtgag tttgatgaag acaattccgg cggcgggaag ctctacgtcg
    26161 gcgtgttcga tcgcaagttg cacctcgccg gggcggagtg gggcgcgtgg acggtcgaca
    26221 agaacgccga gtatcacggt ggtagcaaga cgccttcgcc gaagccgatc gcaccgcgcg
    26281 ttgaagagct ggtgaagtac acggacacgg ataacaacgg attcctggac acgattgaat
    26341 atgactatga cggtgatcga acgatcgatc tgcgcgtgtc gctgctcgat taccgcactc
    26401 cgagcaatcc gcaccctgat gttgtgccgc tgatcgatac gcacaaggaa ggttggaagg
    26461 ggctcaacgt tttgttcggg cagatcgcga gccagtcgtt ccaggaaggc ttgacggtct
    26521 atcgcgcggc gtggcgtcgc gggttgacga cgcccgagat cgacaagctc gcctccgcct
    26581 cggccattgg agagcggtac gatcacggtt attggataaa agagaagatc tttcgggaga
    26641 ttcggaaacg ggttcgtggc accaaattgg agaaagatct cacaaaactc tactacaccg
    26701 gccagttcga ggaatatgcc cgccgcattg cagaggttcc ggcgaagtga tccacagatt
    26761 acgcagatga cgcagataat ctgtgtaatc tgtggatgaa atattttaat cttcctataa
    26821 agattatagc tttgactctt ggcccaaccc tccgtagcat ccttgcttgc ttgcccacag
    26881 gaggggaacc ccatgccaca gaaccgagtc agcatctttg atacgacttt acgtgacggc
    26941 gaacagtcgc ccggctgctc gatgaacctg accgagaagg tgcggatggc cggtaagctg
    27001 caggatctcg gtgtcgacat catcgaagcc ggctttccga ttgcttctga cggtgacttt
    27061 gccgccgtca aagccatcgc cgcccagtgc cggcacgtta aggtggccgc gctctgccgc
    27121 accgccgaac aagacgtcgc gcgcgctgcc gaggcgctga agtatgctgc tcgtccacgt
    27181 attcacacgt tctgtgccac gtctgacatc catctcgaac acaaactgaa gaagacgcgc
    27241 gccgaagtga tcgccatgac gcgcgccgcg gtgcgacagg cacgcagcta cgccgatgac
    27301 gttgagtttt cagccgaaga tgcgacgcgc agcgatatcg attacctgtg cgaagtgctc
    27361 gacgctgcgg ttgatgccgg cgcgtcagtt ttgaacgtgc cggacacggt tggatacacg
    27421 acgccaaacg aatacacaca cctcgttcaa caagtgcgcg agcgtgtagt gagggatcgg
    27481 gacgacgtga tcatcagtgt tcactgtcac aacgatctcg gactcgcagt cgcaaacagt
    27541 ctcgcggcga tcgaagcggg ggcgcgacag gttgaatgta cgatcaacgg catcggcgag
    27601 cgcgcgggca atgcgtcgct cgaagagatc gtgatggcgc tacatgtacg cgccgatcgt
    27661 ctgccgttcg agacgggcat caaaaccact gaactttatc cgaccagcca actgctttcg
    27721 agcatcgtcg gaattgacgt gcaaccgaac aaagcaatcg ttggccgcaa tgctttcgca
    27781 catgaggccg ggattcacca gcacggcgtg atcaataatc cgctttgtta tgagatcatg
    27841 acgccggaat cagtcggcgt gcccgcaaac tcgctcgtgc tcggcaagca ttccggacgc
    27901 cacgcactct cgttgcgcta tgctgagctc ggttatgacg tcaccgcggc ggagctcgat
    27961 gcggcataca tgaggtttac aaacctggcg gatcgcaaga agaacatcta cgatcaggat
    28021 ttgatttcat tgattggcaa cacccacaac gttgtagcag cttagaaaga agatttatgc
    28081 aagccagaat tacgattctt cccggggatg gaattggacc agaagtcacc accgaagcag
    28141 tcagagtact gcaaaccatc gcagcaattc agggttaccg gttcgaattt gaagagcacg
    28201 ccatcggcgg cgttgcgatc aagcgctttg gctcgccact cccgcgaacc acgctcgacg
    28261 cagcgcttgc gtcagacgcc gtgttgctcg gcgcggtagg gtcgccggaa ttcgatcgcg
    28321 tgccgccgaa tcaacgtccc gaagcaggtc tgttgttgct gcgaagcgcg cttggcgggt
    28381 atgcgaacct gcggccagca atcgcttact cgtcaatcgc ggcttgttcg cccgtgcgca
    28441 ctgaagtggc gcacgacgcc gatattctca tcgtgcgcga gttgcttggt ggtctttatt
    28501 tcggcgagcc gcgtggtatt gatgctgatg tcgcgacgaa tacgatgcgc tattcagtcg
    28561 aggagattga gcgcgtcgcg agggtcgcgt ttgaagccgc gcgcaaacgt cgcggcaaag
    28621 tcacttctgt cgataaggcg aacgtgctcg agacttcgca gctctggcga cagactgtga
    28681 ttcgcgtgtc gcaggattat cctgacgtga tgctcgaaca tctttacgtc gatgcgtgtg
    28741 cgatgcactt gatcgcgaac ccgcggcgct tcgatgtgtt gttaaccgag aatctctttg
    28801 gcgacattct ttccgacgag gcggcggtga ttaccggatc gttgggcatg cttgcgtcgg
    28861 cgtcgatcgg cggcgaagtc gggctttacg agccggtgca tggctcggcg cctgatatcg
    28921 tcgggcagaa cgcggcgaat ccctgcggcg cgatcgcgtc ggcggcaatg atgttgcgct
    28981 actcgttcga tctcgaacag gacgcgaaag acatcgagct ggcaattcaa gacgtgctcg
    29041 aagccgggta tcgcacgccc gatattgctg aaggtacggg tggatatgtc gtgacgacga
    29101 gcgagtttgg tgagttgatt tgtcaggccg tcaccgagat cgcggatatg cgtcacgcgt
    29161 atcatgctgt gtaagtttat tagacaggat ttacaagatt gttcaggatt gacatgtaaa
    29221 tcttgatcga tcttgtaaat cttgtctaaa actatttcta tgcccacgct attcgataaa
    29281 ctttggcagt cgcatgtcgt acgtgaagca cgcggcgagc ccacgctcct ttacatcgat
    29341 ctacatctca ttcacgaggt gacgtcgccg caggcgtttg acggtttacg ccaggcaggc
    29401 cgcaaagtga gacgaccgga tctcactttc ggcaccgtcg atcacaacgt tcctaccact
    29461 gaccgcagtc tgccgatcgc cgacgctatc gccgccaatc agatcgagac actgcgccga
    29521 aactgtagcg agtttgggat tcaactctac gacatcgact cgcccgagca gggcatcgtt
    29581 cacgtcattg gaccagagct tggactgacg cggcccggta tgacgatcgt ctgtggcgat
    29641 agtcacacga gcacgcatgg cgcgttcggg gctctcgcgt tcggcattgg gacaagcgag
    29701 gtcgagcatg ttttggcgac gcaatgtctg ccgcagaaca agccgttgac gatgaaagta
    29761 gaaacgcgcg gcgcgctact ggaaggtgtg gcggcgaaag atctggcgct cggcatcatc
    29821 ggcaagatcg gtactgacgg cgcgaccaga tgcgtgatcg aatacgcggg cgatgctgtg
    29881 cgggcgcttt cgatggaagg ccgcatgacg ttgtgcaaca tgagtattga agccggcgcg
    29941 cgtgcgggga tgatcgctcc cgatgagacg acgttcgcgt atctcaaggg tcgtcgtttt
    30001 tctccggaag gcgacgtgaa ttactggaag acattgcaca gcgatacgga tgcgaaattc
    30061 gatcgcacag ttgagatcga tgcagcacaa ctcgagccgt ttgtgacgtg ggggacgagt
    30121 ccgggcatgg tcgtgccggt gacgagttat gtgcccgatc cggcggatga ggccgcggag
    30181 cgcgcgctcg agtacatggc cctgaagccg cgcacaaaca ttgaggacat aacaatcgat
    30241 cgcgcgttca tcggctcgtg tacgaatgcc cggatcgagg atctgcgcgc ggcggcgcgt
    30301 gtagtgaagg ggtaccggat caactcgtcc gtgagcgcga tggtcgtgcc gggttcgcag
    30361 gaggtgaagc gcgcagcgga acgcgagggg ctcgatcgga ttttccatga ggcgggattt
    30421 gagtggcgcg aagccggttg ttcgatgtgt ttgggaatga atgctgacat cctgcagccg
    30481 ggcgagcgct gtgcgtcgac gagtaatcgc aacttcgagg gaaggcaggg gcgcggcggc
    30541 cgcacgcacc tcgtgagtcc gatgatggcg gcggcggcgg cgatcaaggg acatttcacg
    30601 gacgtgcgag attgggactt taaatagcca cggatgaaca cggatgaaag cgtttaagaa
    30661 acataccggg cttgtagccc cactcgatcg cgccaacgtc gacacggatc agatcattcc
    30721 gaagcagttc ctgaaacgca tcgagcgcac cggcttcgga gagtttctct tttacgactg
    30781 gcgctacacc gcggggggcg cactcgatcc gtcgtttcca ctgaa
//
LOCUS       DASSFQ010000240         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2200868, whole genome shotgun sequence.
ACCESSION   DASSFQ010000240 DASSFQ010000000
VERSION     DASSFQ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200868"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..732
                     /gene="hisG"
                     /locus_tag="VFH96_08805"
     CDS             100..732
                     /gene="hisG"
                     /locus_tag="VFH96_08805"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET6974108.1"
                     /translation="MITIAIGKGRMQESALALLTRAGIRLSEEAVHSRRLALVDESGQ
                     YRFIFVKPADVPVYVEHGIADCGIVGRDVLLESEADLLLPLDLGISRCRIVVAAANAD
                     AMTKVQGMIRVATKYSQIARAHFGARGIPVEIIQLSGSVEIAPALGLADMIVDLVETG
                     ITLRENGLTVVEEIAVSTGRLVVNRASYQLKTEEVAGLVRALEATLNGKQ"
     gene            729..2057
                     /gene="hisD"
                     /locus_tag="VFH96_08810"
     CDS             729..2057
                     /gene="hisD"
                     /locus_tag="VFH96_08810"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET6974109.1"
                     /translation="MRIFLPTERSALQPWLEHLAARSVALDVELMKQVASIIGDVRAR
                     GDEALIEYSARFDRVELTASQLRISRDELQRFAKGVNEAVRVALREAIGNVRAFHERQ
                     VEQSWRFDPVDGVQLGQRITPLESVGLYVPGGTAAYPSSVVMNVVPAQVAGVERIVVT
                     TPPRTLSENPAVAAALLELDVDEVYAVGGAQAIAALAFGTETVPCVDKITGPGNKYVA
                     AAKKLVFGVVGIDSIAGPTEVVIVADETARPDFIAADLLAQAEHGEDASAILITDSER
                     LAQSVAREVKLQSETLPRQEIVETSLKDYGAIIVVERLAEAPSLVNELAPEHVEIVTS
                     DDEAVAGGIRHAGAIFFGGYTPEAVGDYLAGPNHILPTGRTARFSSALGVYDFVKRTS
                     LLRYSEEAFRNVADSVSVLAECEGLEGHSKSALIRKKISRKGAKKDAKLA"
     gene            2102..>2359
                     /locus_tag="VFH96_08815"
     CDS             2102..>2359
                     /locus_tag="VFH96_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974110.1"
                     /translation="MNPLESIKPAVRALRGYTLAPHRASLKVNQNENPWDAPARIKEE
                     VLRRFTERRWSRYPEFVPSDLHERLAAFAGWKSDGVLAGNGS"
BASE COUNT          469 a          534 c          807 g          549 t
ORIGIN      
        1 tagtttgacg ggaagttttg gacgcgcaga gcctgcggtc ggctttgtgc tcaatctcga
       61 tgcgcttacg gaagttctcg ggcgtagtaa gcaagactga tgattacgat tgcgatcgga
      121 aaaggacgaa tgcaggaaag cgcgttggcg ctgttgacgc gcgctggcat tcgtttgagt
      181 gaggaggcgg tgcattcgcg gcggctcgcg ctcgtcgatg aatcgggcca gtatcgattc
      241 atctttgtga agcctgcaga cgttccggtt tacgtcgagc atgggattgc tgactgtggc
      301 atcgttggcc gggacgtgtt actggagtcg gaggccgatc tgttgttacc gctcgatctt
      361 ggcatctcgc gttgtcgcat cgtcgtggcc gcagcgaatg ctgatgcaat gacgaaggtt
      421 cagggcatga ttcgcgtcgc gacgaagtat tcacaaattg cacgcgcgca ctttggcgcg
      481 cgcggcattc ccgtcgagat tattcagctt tcggggtcgg tcgagatcgc accggcgctt
      541 gggctcgcgg acatgatcgt tgacctggtc gagactggca taacgttgcg ggagaacggg
      601 ctgacagtgg tcgaagagat cgccgtttcg accgggcggc tcgttgtgaa ccgcgcgagt
      661 tatcaattga aaactgaaga agtcgcgggc ctggtgcgag ctttggaagc gactttgaac
      721 ggcaagcaat gaggatcttt cttccaactg agcgaagtgc gctacagccg tggctggagc
      781 atttagcggc gcgttcagtg gcactcgatg tggagttgat gaagcaggtg gcgtcgatta
      841 tcggcgacgt gcgcgcccgt ggagacgagg cgttgatcga atacagcgcc cgcttcgatc
      901 gcgtggagtt gacggcgtca cagttgcgaa tcagcaggga tgagctacaa cgtttcgcga
      961 agggcgtgaa tgaagcggtg cgagttgcac ttcgtgaggc tattggaaac gtcagagcct
     1021 ttcacgagcg gcaggttgag caatcgtgga ggttcgatcc ggttgacggt gttcagttgg
     1081 gccaacgaat taccccgttg gaaagtgtcg ggttgtatgt tccgggaggt actgcggctt
     1141 acccgtcgtc agtggtgatg aatgtggtac cggcgcaggt tgctggtgtg gagcgaatag
     1201 tggtgaccac gccaccgcga acgctgtccg agaatcctgc ggttgcggct gctttgttgg
     1261 agcttgatgt cgacgaggtg tatgcggttg gtggagccca ggcgatagcg gcgctcgcat
     1321 ttggcaccga gactgtgccg tgtgtcgata aaatcactgg gcccggtaac aagtatgtgg
     1381 cggcggcgaa gaagctggtg tttggcgtgg ttgggattga ttcgattgct ggtccaaccg
     1441 aggtggtgat cgttgctgac gagacggcgc ggcccgattt cattgctgca gatctactgg
     1501 cgcaggcgga acatggtgag gatgcgtcgg cgatcttgat taccgatagt gagcgactgg
     1561 cgcagagtgt ggcgcgcgag gtgaagttac agtcagagac gttgccacgt caagagatcg
     1621 ttgagacatc gctgaaggac tatggcgcga tcattgtggt tgagaggttg gctgaggcgc
     1681 cctccttggt aaatgaattg gcgccggagc atgttgagat tgtgacgagt gatgatgagg
     1741 ctgtggctgg agggattagg catgcgggag cgatcttctt cggtggttac acgccggagg
     1801 cggttggtga ttatctggct ggtccaaatc atattttgcc gactggacga acggcgcggt
     1861 tttcgtcggc gcttggggtt tatgatttcg tgaagcgcac gagcttgttg cggtattcgg
     1921 aggaggcgtt cagaaacgtg gcggacagcg tgagcgtgct ggcggaatgt gaaggcttag
     1981 agggacactc aaagtccgct ctaattcgga aaaagatctc acgcaaaggc gcaaagaaag
     2041 acgcaaagct cgcttaaatc gtcttctttg cgcctttgcg tgaaaaatat ctgctcgagt
     2101 tatgaatcca ttggaatcca tcaaacctgc cgttcgcgca ttgcgtggct acacgctcgc
     2161 gccgcatcgc gcgtcgctga aggtcaatca aaacgagaat ccgtgggatg ccccggcgcg
     2221 catcaaggaa gaagtgctgc gccgatttac tgagcgaaga tggtcgcgct atccggagtt
     2281 tgttccttca gacttgcacg aacggctcgc agcgtttgcc ggatggaagt ctgatggcgt
     2341 gctcgccggt aacggctcc
//
LOCUS       DASSFQ010000241         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1602755, whole genome shotgun sequence.
ACCESSION   DASSFQ010000241 DASSFQ010000000
VERSION     DASSFQ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602755"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="VFH96_08820"
     CDS             <1..1501
                     /locus_tag="VFH96_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974111.1"
                     /translation="TILYALIFRLSKNILFQARFQLTIDVLIVSWLVWNSSTVQSPYV
                     ALYIVIIAVSSLFLGPREAIVACVGCAAAFTLCALQITGFGAQTDPTKLVGGSLSQTI
                     QWVGLFDVAFFVVGLLSARLAERQSRSDVRLAAATQSLANLRALHERIVASIRSGLVT
                     TDLEGRIFTFNVAAQEITGYQEETIRGQDASILFGDMKDHIAVTLSALAKGANSPRFE
                     TNCLTSEGMRIRLGYSISPLSSEAGDTTGMVITFQDLTQVRSLEETSRRQDRLAAIGR
                     MAASIAHEIRNPLAAMRGSIQMLHAEVDSDSSQAQLMEIILRESDRLNRIITDFLSYA
                     RPRSLVQARVDVGDLLHQTFALMRHSPEINSNQSIVEELPDEPLFAEADEGQLKQVFW
                     NLARNALQAMPQGGTLRATLDKNSHNRLRISFADTGRGMSPDQVEHLFEPFSSTTGGT
                     GLGLSIVYQIIRDHGGTINVRSLVGQGTTITVELPMATNNNDLASEQSN"
     gene            1523..>2324
                     /locus_tag="VFH96_08825"
     CDS             1523..>2324
                     /locus_tag="VFH96_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6974112.1"
                     /translation="MANLLIVDDELGMRQFLTHLFQREGHVVHVAGNGLEALDEIRQH
                     TPDLIISDIRMPDMGGVDLLRAARELLPDVEVIMMTAFANVDTAREAFLLGAYDFVQK
                     PFDNDLLKETVARALNKISLVKEKEALLEENKALIQGQRTRGKLGNIIGRSGRMLSLY
                     QMIETVAQVQSTVLVTGESGTGKELVARAIHDLSPRAERPFVSVNCGAFTETLLESEL
                     FGYVKGSFTGANANRKGLFEAANSGTIFLDEIGEMSPAMQVKLLRVLQE"
BASE COUNT          509 a          648 c          648 g          519 t
ORIGIN      
        1 cacgattctt tacgctctga ttttccgtct ctcaaaaaac attctctttc aggcgcggtt
       61 tcagctaacg attgacgtct taatcgtcag ctggctggtc tggaattcca gcacggttca
      121 gtcgccctac gtggcgctat acatcgtcat catcgcggtt tcgagtctct ttcttggacc
      181 acgcgaagcg atcgtggcgt gtgttgggtg tgcggcggcg ttcactttat gcgcgctcca
      241 aatcactggt tttggagcgc aaactgatcc gacgaaactg gttggtggct cgctatcgca
      301 aacgattcaa tgggtcggac tgtttgatgt cgcattcttc gtcgtcggcc tgctgtctgc
      361 gcgacttgcc gaacgtcaat cacgatccga tgtgcgactc gctgccgcaa cgcagtcact
      421 cgccaacctg cgcgctctgc acgaacgtat cgtcgcctcg attcgctccg gcctcgtgac
      481 caccgacctc gaaggacgca tcttcacttt caacgttgcg gcacaggaga tcactggtta
      541 tcaggaagag acgattcgcg gtcaggacgc ctcgatcctg tttggcgaca tgaaggacca
      601 tatcgcggtc acgctcagcg ccctcgccaa aggtgcgaat agtccgcgct tcgaaacgaa
      661 ttgtttgacc tcggaaggaa tgcgaattcg tcttggttac agcatctcgc cgttgtcgtc
      721 cgaagcgggt gacaccacgg gcatggtgat cacgtttcag gatctgacgc aggttcgcag
      781 tcttgaagaa acatcgcgac gacaggatcg gctcgccgct attggtcgca tggccgcctc
      841 gatagcgcac gaaattcgaa acccattggc tgccatgcgc ggctcgatcc agatgcttca
      901 cgcagaggtc gacagcgact cgtcgcaagc gcaattgatg gagatcatcc tgcgcgagtc
      961 ggatcgactg aaccgcatca tcaccgattt tctcagttat gcgcggccgc gttcgctcgt
     1021 tcaagctcgc gtggacgtcg gcgacttgtt gcatcaaaca ttcgccctca tgaggcacag
     1081 tccggagatc aactcgaacc agagtatcgt cgaggaatta ccggacgagc cgcttttcgc
     1141 cgaagctgat gaaggccagt tgaagcaggt gttttggaat ctggcgcgca atgctcttca
     1201 ggcaatgccg cagggcggca cgttgcgtgc gaccctggac aagaattccc acaaccgcct
     1261 gcgcatctct tttgctgaca ctggccgagg catgtcgccc gatcaggtag aacatctttt
     1321 cgagcctttc tcctcgacga ccgggggcac cggcctgggg ctgtcaattg tttatcaaat
     1381 cattcgcgat catggtggta caatcaatgt ccgcagtctc gtgggtcagg gaacgacaat
     1441 tacagttgaa ttgccaatgg cgactaacaa caacgatctc gcttcggaac agtcgaacta
     1501 attaccgatt ggaaatgaaa tcatggccaa tcttctgatc gttgacgacg aacttggaat
     1561 gcggcagttc ctgacgcatt tattccagcg cgaaggccac gttgtgcatg tcgcgggtaa
     1621 cggtctggaa gcgctggatg aaatacgcca gcacactccc gatctgatca tctccgacat
     1681 caggatgcct gacatgggcg gcgtcgatct gctgcgcgcg gcgcgcgagc tacttcccga
     1741 cgtggaagta ataatgatga ccgcgttcgc aaacgtcgac acagcgcgcg aagcgtttct
     1801 gctcggtgct tacgacttcg ttcagaagcc gttcgataac gacctattaa aagagacggt
     1861 cgcgcgagct ctcaacaaaa tctccctcgt taaagagaaa gaagcgctac tcgaagagaa
     1921 caaggccctc atccagggcc aacggacgcg cggcaagctg ggcaacatca tcggccgtag
     1981 tgggcggatg ctttcgctct accaaatgat cgagacggtg gcgcaggtgc agtcgacggt
     2041 cctggtaacg ggtgagtccg gcaccggaaa agaactcgtg gctcgcgcta tacacgattt
     2101 aagcccgcga gctgagcggc cgtttgtctc cgtaaactgc ggcgctttta ccgaaacgct
     2161 gctggaatca gagctgtttg gttacgtgaa gggctcgttc accggcgcga acgcgaatcg
     2221 aaaaggactg tttgaagcgg ccaacagcgg cacgattttc ctcgacgaga tcggtgaaat
     2281 gtcgccggca atgcaggtca aactgctgcg cgtattgcag gaac
//
LOCUS       DASSFQ010000242         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_111535, whole genome shotgun sequence.
ACCESSION   DASSFQ010000242 DASSFQ010000000
VERSION     DASSFQ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111535"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="murD"
                     /locus_tag="VFH96_08830"
     CDS             <1..899
                     /gene="murD"
                     /locus_tag="VFH96_08830"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET6974113.1"
                     /translation="DDGWTVAELSSFQLETIKTFRPAVAVVLNVTPNHMDRYETLNDY
                     AAAKHRIFMNQTAEDTAVLNADDPTVSSWASGLRARVVQFSVKKEVENGVFLRGRELI
                     YRGQVLLNVDEMKLRGMHNVENVAAAFAAGIAAGASIESMNGTAKRFDPVEHRLEFVT
                     EIEGVKFYNDSKATSVDATLKALEAFAHEDGKVVLILGGKGKKAPYAPLEPLVRAKVR
                     KLVLIGEDAETIANELGSSAPFERASDMKDAVARSYAAAEKGDVVLLAPACASFDMFE
                     SFEHRGKVFKEEVLSTDFTESA"
     gene            917..2095
                     /gene="ftsW"
                     /locus_tag="VFH96_08835"
     CDS             917..2095
                     /gene="ftsW"
                     /locus_tag="VFH96_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET6974114.1"
                     /translation="MARKLAPDKWLFAATVGLALFGVVMVYSASAVLAQKENGNQFHY
                     VLKQGVWVAIGFVVMLLAMQFNYQQLKNRRLVYALLVICTIGLICVFGFSSINGAHRW
                     IKLPGVNLQPSEISKLTLIIFLSYFLEKRAGEEGDFWRTFVPCGVVTGWLAALVVIEP
                     DFGTAMMLVLIFTVVIYTAGARVRHLALAAAPALVVAAGLLILVPWRLKRLMTFLDPW
                     ADQQGGGFQVVQSLIAIGSGGTNGLGFAQGKQKMLFLPFAHSDFIFAVVGEELGLVGA
                     LAVVAVFALFLWRGVRTALLAPDRFGMLLSLGIVTSIVAQALFNISVVLSLVPTKGIP
                     LPFISYGGSSLVPTLAAVGILLNVSQHASGSLDVGLFEKATTKLRGERKQLNETAFDH
                     "
     gene            2085..3185
                     /gene="murG"
                     /locus_tag="VFH96_08840"
     CDS             2085..3185
                     /gene="murG"
                     /locus_tag="VFH96_08840"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET6974115.1"
                     /translation="MIIEMRVMIAAGGTGGHIYPGIAVAQEIMRRDASSSVHFVGTAR
                     GLETRLVPQAGFELSLIESAGLKNVSLGARMKGVALLPKSFISARRLMRLFQPDVVVG
                     AGGYVSGPVVLTAAITNRPTLVMESNALPGWTNRVLARFVDRAAVSFEQALPYFRGKA
                     KVTGNPVRREFFEIPPKRREPGKFSLLVFGGSQGARAINEAIVAALPKLKELAVELRI
                     KHQTGPADFEKVKAAYEAASWSERGDVRSYIDNMMQDFAEADLVICRAGATTTAELIA
                     AGKASIMVPFPFAADDHQRKNAEALQSAGAARMILQQDLSGERLAAEIEKLVQSPEEL
                     DRMEEASRKLAHGDAAAAAVDMIEELSIKRKL"
     gene            3252..>4009
                     /locus_tag="VFH96_08845"
     CDS             3252..>4009
                     /locus_tag="VFH96_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET6974116.1"
                     /translation="MFRRIQHVHFVGIGGIGMSGIAEVLVNLGFRVSGSDLKRSNVTD
                     RLQQMGVEIFEGHAAENVGHPHVVVRSTAVRDDNPEIIEANRRSIPVIPRAEMLAELM
                     RLKPHTVAVAGSHGKTTTTSMVATVLGHAGLDPTMVVGGVVGAFGSNAHLGTSDLMVV
                     EADESDRSFLMLTPTIAVVTNIDREHMDYYHDMDDVRACFANFVNKVPFYGSSVLCLD
                     DPNVQAIIPLLERRRLTYGLSAQADVSAHGIRHD"
BASE COUNT          826 a         1014 c         1271 g          898 t
ORIGIN      
        1 gcgacgatgg ctggacggtc gcggagcttt cgagttttca gctcgagacg atcaagacct
       61 ttcgcccggc cgtagcagtt gtgctgaacg tgacgccgaa tcacatggat cgttacgaga
      121 cgctcaatga ctacgctgcc gcgaagcacc ggatctttat gaaccagacc gcggaagata
      181 cggcagtgct caatgcagac gatccgacgg tttcgtcctg ggcgagtggt ttgcgtgcga
      241 gggtagtgca gttcagcgtg aagaaggaag tcgagaacgg agttttcctg cgtgggcgtg
      301 agctgattta tcgcgggcaa gtgttgctga acgttgacga gatgaagctg cgtggaatgc
      361 acaacgtcga gaatgtcgcg gccgcattcg cagcaggaat cgcggcgggc gcgtcgattg
      421 agtcgatgaa cggcactgcg aagcgattcg atccggtcga gcaccggctg gagtttgtaa
      481 ccgaaatcga gggcgtgaag ttttacaacg actcaaaagc gacgagcgtc gacgcgacgt
      541 tgaaggcgct ggaagcattc gcgcatgagg atggaaaggt tgtgttgatt ctcggcggta
      601 aagggaagaa ggcgccgtac gcgccgctcg agccgttagt acgcgcgaaa gtgagaaagc
      661 tcgtcctcat cggtgaggat gcggagacga tcgcgaatga actgggaagt agcgcgccat
      721 tcgagcgtgc atcggatatg aaagacgcgg tcgctcgttc gtatgcggct gctgaaaaag
      781 gtgacgtggt tctgctcgcg ccggcatgtg cgagcttcga catgtttgaa agcttcgaac
      841 accgagggaa ggtctttaaa gaagaggtgt tatccacaga tttcacagaa tctgcgtaat
      901 ctgtggatgg ttcgttatgg ctcggaaact agcaccagat aaatggctct tcgcggcaac
      961 ggtcggcctc gcgctgttcg gcgtcgtgat ggtctacagc gcgtccgcgg tgctggcgca
     1021 aaaggagaac ggcaaccagt ttcattacgt gctgaagcag ggcgtttggg tcgcgatcgg
     1081 cttcgtcgtg atgctgctcg cgatgcagtt caattatcaa caactgaaga atcgccggct
     1141 tgtctacgcg ttgttggtta tctgcacgat cggtctcatt tgcgtgtttg gttttagcag
     1201 tattaacggt gcgcatcgct ggataaaact tccgggcgtc aatcttcagc cgagtgagat
     1261 ctcaaaactc acgctcatca tttttctctc gtacttcctc gagaagcgcg cgggtgaaga
     1321 aggagacttc tggcgcacgt ttgttccatg cggtgtggtg actggctggc tcgcggccct
     1381 cgtcgtgatc gaaccggact tcggcacggc gatgatgctg gtgctgatct tcaccgttgt
     1441 gatttacacg gctggcgcgc gcgttcgtca tctcgccctc gctgctgcac ctgctttggt
     1501 cgtagcagcg ggtttgctga tcctggtgcc gtggcgactg aaacgtctga tgacgtttct
     1561 cgatccatgg gccgaccagc agggtggcgg ttttcaggtc gtgcaatcgc tgatcgcgat
     1621 cggatccgga gggacgaacg ggctcggctt cgcacagggc aaacagaaga tgctgtttct
     1681 gccgttcgca cattcggatt tcatctttgc agtagttggc gaagagcttg ggttagtcgg
     1741 cgcactcgct gtcgtcgcgg tgtttgcact ttttctctgg cgtggcgtgc gcacggcatt
     1801 gctcgcgccg gatcggtttg ggatgttgct gagtctcgga atcgtgacga gtatcgtcgc
     1861 gcaggcgctc tttaatatta gtgttgtgct ctcgctcgtg ccgacaaagg gaattccgct
     1921 gccgtttatt tcgtacggtg gatcgtcgct cgtgccgaca ctcgctgcgg tcgggatcct
     1981 gctcaatgtt tcgcagcacg cgagtggatc gctcgacgtc gggctgtttg agaaagcgac
     2041 gacgaagctg cgcggcgagc gaaaacagtt gaacgaaacg gcttttgatc attgaaatgc
     2101 gagtaatgat tgctgccggt ggcactggcg gacacatcta tccaggaatc gcagtggccc
     2161 aggagattat gcggcgcgat gctagctcaa gtgtccattt cgttgggact gcgagagggt
     2221 tagagacccg tttggtgccg caagctggtt ttgagttatc gctcattgaa agtgccgggc
     2281 tgaaaaacgt gagtctcggc gcgcggatga aaggcgtcgc gctgttgccg aagagtttta
     2341 tttcggcgcg acggttgatg cgactgtttc aacctgacgt ggttgtcggc gcgggcggtt
     2401 acgttagcgg gccggtcgtg ttgacggcag cgataacgaa tcgtccgaca ctcgtgatgg
     2461 agtcaaacgc gctgccgggt tggaccaacc gcgtacttgc tcgtttcgtc gatcgtgcgg
     2521 cggtctcgtt tgaacaagca ctgccgtact ttcgtggtaa ggcgaaagtg acaggcaatc
     2581 cggtacgacg cgagtttttt gaaataccgc cgaagcgacg cgagccggga aagttttcgt
     2641 tgctggtctt tggtggctcg cagggggcgc gagcaatcaa cgaagcgata gtggcggcgt
     2701 tgccgaagtt gaaagagctt gcggtggagc ttcgcatcaa gcaccagaca ggaccggcgg
     2761 attttgaaaa ggtaaaggcg gcgtacgagg ccgcgagttg gagcgagcgt ggcgacgttc
     2821 gtagttacat tgacaacatg atgcaagact tcgcggaagc cgatctcgtc atctgccgag
     2881 caggtgcaac gacaacggcg gagctgatcg cagcaggcaa ggcgtcgatc atggtgccgt
     2941 tcccctttgc cgccgacgat caccagcgca agaacgccga ggcattgcag tccgccggcg
     3001 ccgcgcgaat gattttgcag caggatttga gcggggagag attggctgcg gaaattgaga
     3061 agttggtcca atcgccggaa gagttggatc ggatggaaga agcaagtcga aagttggccc
     3121 atggcgatgc ggcggcagcg gccgtagata tgattgagga gctaagtatt aaacgtaagc
     3181 tgtaggacac gattacagga ttttcatgat tgacatgcaa atcctgaaaa tcttgtcaat
     3241 cctgtaagaa aatgtttcgt cgcattcaac atgttcattt cgtaggcatc ggcggtatcg
     3301 gcatgtccgg catcgccgag gtgctcgtca atctcggctt ccgcgtctcg ggctccgatc
     3361 tcaaacgctc gaacgtcacc gatcgcctgc aacagatggg tgttgagatc tttgaaggac
     3421 acgccgctga gaacgtcgga cacccacacg tcgtcgtcag atcaactgcc gtgcgcgacg
     3481 acaaccccga gatcatcgaa gcaaaccgcc gctcgattcc agtgatcccg cgcgctgaga
     3541 tgcttgccga gctgatgcga ctcaaaccgc acaccgtcgc cgtcgcgggt tcgcacggaa
     3601 agacaactac gacttcgatg gttgcgacag tcctgggcca cgccggactc gatcctacaa
     3661 tggtcgtcgg cggtgtcgtg ggcgcgttcg gatcgaatgc ccacctcggc accagcgact
     3721 tgatggtggt ggaagcagac gagagcgatc gctcgtttct gatgttgacg ccgacgatcg
     3781 ccgtcgtcac caacatcgat cgcgagcaca tggactatta ccacgacatg gacgacgtgc
     3841 gcgcgtgctt cgcgaacttc gtcaacaaag tgccgtttta tggttcgtcc gtcttatgtc
     3901 tcgacgatcc aaacgtgcag gcgatcattc cgctgctcga gcggcgccgc ctgacctacg
     3961 gcttgagcgc ccaggctgac gtttccgcgc acggcatccg ccacgacaa
//
LOCUS       DASSFQ010000243         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1743344, whole genome shotgun sequence.
ACCESSION   DASSFQ010000243 DASSFQ010000000
VERSION     DASSFQ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743344"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="VFH96_08850"
     CDS             <1..1761
                     /locus_tag="VFH96_08850"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974117.1"
                     /translation="DEPKPLSQMRPGRIPPKLQAILDKALAKDPRNRYQKISYFANDL
                     REIIRELGSDSLPGLDESTTPVAPKHLKQESPVTRALRWLTRNKSGESSSSQRSGGES
                     QSSPLTSLGDRDRKSVAILPFKNVGNDREIDFYQFSLADAVITELARVRSLVVRPSSV
                     IVKYQNKAVDAAEAGRELLVDAILTATFLRAGDRLRVTAQLLDVRTSEILWSERIDAD
                     ASDIIGVQDTIVQRIVEGLRLELSPDEKVELARKTTADAAASEEYLRGRDCLASFIYH
                     TIEREHLDAAIEHFRRAIEIDPNFALAYSALGSCYVNRVLKGLGQSGDHEKAKNAFRK
                     ALTLDPRLLEARMHMVFIYLTGGQKQKAREAVDALRNEYPNDPGVQFVRGVVARLDSD
                     YEKALRSFDRMVRLNPGEKVLASYNRARIFMYQREYEEAIRELDQGEAIEPEHPLIKV
                     FRARVLYYKGEVDEAIKILQQVLQRHPQIDGVRPILAIALSAQGQHTKANEQLTPKVR
                     QVAEADYDISYWLASAYLLQGRQVEALRWLETAIGLGNENYKWFESDPNWTDMHEDPR
                     FVELMNRIKSNREQPGDRSE"
     gene            1758..2552
                     /locus_tag="VFH96_08855"
     CDS             1758..2552
                     /locus_tag="VFH96_08855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET6974118.1"
                     /translation="MRTFVVGDIHGRCAQLLNLLDMLPRDPENDTLVFLGDLIDRGAD
                     APGCVAHILKLCRENPERVICLRGNHEQMLMDFLDGRSNLWLSAVVGGERTFEQYTGH
                     PVRVDSEKELEEMRALFERSFPAEHLAFMEKMPFYYEDEFAIYVHAGLDEGKHPREST
                     QMSLLWMRDMDFYKNYRGKPCVFGHTPTPLLPLRGRLGRHGIYISHSAVGLDTGYNHH
                     SPLSCLSLPDFNLYQTYVDGREETYQITSFIPEALKEMQRKAGIVN"
     gene            complement(2595..>2872)
                     /locus_tag="VFH96_08860"
     CDS             complement(2595..>2872)
                     /locus_tag="VFH96_08860"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HET6974119.1"
                     /translation="WQDRSRRRKMKLKPVESEMLVAAGYDEKSHSLTAVFRTGETYRY
                     KNVPLFVYERLMSAESKGKFMHKYILNRFDYERVTTTTKFREKLKHG"
BASE COUNT          652 a          759 c          815 g          646 t
ORIGIN      
        1 gatgagccca agccgctgtc gcagatgaga ccggggcgca tacctccaaa gttgcaggcc
       61 atactcgaca aagccctcgc aaaggatccg cggaatcgct accaaaagat ctcgtatttc
      121 gccaacgacc tgcgtgagat cattcgtgag ctgggcagtg attcactacc gggcctcgat
      181 gaaagtacga cgcccgttgc tccgaaacat ctcaaacagg aaagccccgt cacacgtgca
      241 ctgcgttggt tgacgaggaa caagagcggc gagagctctt cgagccagag atcaggtggt
      301 gagtcgcagt cgagcccgtt gacgagtctc ggcgatcgcg atcgaaagag tgtcgcgatt
      361 cttccgttca agaatgtcgg gaacgatcgc gagatcgatt tttaccagtt ctcgcttgcc
      421 gatgcggtga ttactgagct cgcgcgcgtg cgatcgttgg tcgtgcggcc gtcgtcggtc
      481 atcgttaagt atcagaacaa ggccgtcgat gctgctgaag ccggacgcga gctcttagtt
      541 gacgcgattc tcacggcaac gtttctgcgc gctggcgatc gcttgcgtgt aacggcgcaa
      601 ctactcgacg tgcgaacgag tgagattctc tggagcgagc gtatcgatgc cgatgccagc
      661 gacatcatcg gcgtacagga cacgatcgtg cagcgcatcg tcgagggact gcggctggag
      721 ttgagtccgg atgagaaagt cgagctcgcg cgaaagacga ctgctgatgc cgccgcgtca
      781 gaagaatacc tgcgtggtcg cgactgtctt gcgtcgttta tttatcacac gatcgagcgt
      841 gagcatctcg acgcggcgat cgaacacttc cggcgcgcga tcgagatcga tccaaatttc
      901 gcactcgctt actccgcgct gggaagttgt tacgtcaatc gcgtcttgaa aggcttgggc
      961 cagtctggcg atcatgagaa ggccaagaat gcgtttcgaa aagcgctcac gctcgatccg
     1021 cggctgctcg aagcgcgcat gcacatggtg ttcatctatc tcaccggtgg gcagaagcaa
     1081 aaggcccgtg aggcagttga cgcgctgcgc aacgagtatc cgaatgatcc gggtgtgcag
     1141 tttgtgcgtg gcgtggtcgc gcggctcgac agcgattacg aaaaggcgct gcgcagtttc
     1201 gatcgcatgg tgcggctcaa tcccggtgaa aaggtgctgg cgagttacaa tcgtgcgcgc
     1261 atcttcatgt atcaacgcga atatgaagaa gcgatacgcg agctcgatca aggcgaagca
     1321 atcgagcccg aacacccgtt gatcaaagtc ttccgtgcgc gcgttcttta ctacaaaggt
     1381 gaggttgacg aggcgatcaa gattcttcag caggttttgc aacgacatcc gcaaatcgat
     1441 ggcgtgcgcc ccatcctcgc gattgcactg agcgcgcagg gacaacacac caaggcaaac
     1501 gagcaactaa cgccgaaagt caggcaagtg gcggaagccg attacgatat ctcctactgg
     1561 ctcgcttcgg cttatctatt gcaaggccgt caggtagaag cgttacgatg gctggagacc
     1621 gcgatcggtc tcggcaatga gaactacaaa tggtttgagt cagatccaaa ctggaccgac
     1681 atgcacgagg atccacgttt cgttgaattg atgaaccgca tcaaaagcaa ccgcgagcaa
     1741 cccggggacc ggtctgaatg agaacattcg tcgttggcga catacatggc cgttgcgcgc
     1801 agctcctcaa tctcctcgac atgctgccgc gagatccgga aaacgacacg ctcgtttttt
     1861 tgggcgacct gatcgatcgt ggcgccgatg cgccgggctg cgtcgcgcac attctcaagc
     1921 tctgccgcga gaatccggag cgagttatct gcctgcgtgg aaatcacgaa cagatgttga
     1981 tggattttct tgacggccgt agtaacctct ggctctcggc agttgtgggc ggcgaaagaa
     2041 cgtttgagca gtacacaggt catccggttc gagtcgatag tgaaaaggaa cttgaagaga
     2101 tgagggcgtt gtttgagcgc tcgtttcccg cggagcacct cgcgttcatg gagaagatgc
     2161 ctttttacta cgaagacgag tttgcgattt atgtgcacgc cgggctcgac gaaggcaaac
     2221 atccgcgtga gagcacgcag atgtcgctgt tgtggatgcg cgacatggat ttttacaaga
     2281 actatcgcgg gaagccctgc gtgtttggac acacgccgac gccgctgctg ccattacgcg
     2341 ggcgtctcgg tcgtcacggc atctacatct ctcacagcgc ggtcggcctc gatacaggct
     2401 acaaccatca ctcgccgcta agctgtcttt cgctgcccga tttcaatctt tatcagacgt
     2461 atgtcgatgg tcgtgaggaa acctaccaga tcacttcatt cattcctgag gcgctgaagg
     2521 aaatgcagcg caaggccggg attgtaaatt agccacggat aacacggatt ttaaatcgga
     2581 tttttaaatc cgtgtcatcc gtgtttaagt ttttctcgga atttcgtagt cgtcgttact
     2641 cgctcataat cgaagcgatt gaggatgtat ttgtgcatga actttccctt ggactcagcg
     2701 ctcatcaatc gttcgtatac aaacaatggg acgtttttat aacgatatgt ttcgccggtt
     2761 ctgaagacgg ccgtcaggct gtgcgacttt tcatcatagc ccgcagcaac cagcatttcc
     2821 gactctacag gtttcaactt catcttcctt ctcctggatc gatcttgcca ta
//
LOCUS       DASSFQ010000244         6118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2202439, whole genome shotgun sequence.
ACCESSION   DASSFQ010000244 DASSFQ010000000
VERSION     DASSFQ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202439"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VFH96_08865"
     CDS             <1..1064
                     /locus_tag="VFH96_08865"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HET6974120.1"
                     /translation="LSERSKSYENNSDDQKSFDEDFVRIVRYLESEVPPQVQGVAIFA
                     CSADSDWFVVGLFEAPFERNRLFVSDRPHLYPLARLVDQYRRYAVVLADTNRAQIFVF
                     AAGRAVEQEQVENVKTKQTKVGGWSQARYQRHVQNYHLQHAKEVVDTLEKIVRDENIE
                     HVILAGDEAVVIPMLREQMPKTLEEKIIDALSLGIDTPEHELLDESLTAFQRHDSLTD
                     MEKVERLLNEYRADDLGVAGVSETLAALSNGQVEEMLIAAKAESLQYDKEEVEKVLKL
                     YDVDGALPEELDQRTVADELVRRANVLSSARVTFIEDSTRLERVGGVGAFLRYRISEP
                     QVNADKRGSDQRLSASIRG"
     gene            complement(1061..2428)
                     /locus_tag="VFH96_08870"
     CDS             complement(1061..2428)
                     /locus_tag="VFH96_08870"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6974121.1"
                     /translation="MSITSDFVSIPNIMTTTAETMTGEQIVELCRHHTLFEWSAQAAV
                     DPIPVARAKGIYFWTPEGKRFIDFNSQLMCVNIGHGDERVIRAIKEQADTLCYANPFM
                     ATETRARLSVKLAELTPGDIDAFFFTNGGAESNENAIRIARLYTGRHKIGARYRAYHG
                     GTAGALTLTGDPRRWAAEPGIPGVFRIPDFYHGIQKGWDTTEDVLSRTEEVLELEGPN
                     TIAALIVEPVVGTNGILIPPDGYMQGLRALCDKYGILLIADEVMAGFGRTGEWFSIDH
                     WKVVPDLISMAKGLSSAYVQLGAVGMRRQIADHFEKNVFYGGLTYNSHPLACAAALAN
                     IAVYEEDNLIENARRLGTVMRGLLEDLEQRHPSVGATRNIGLFGIVELVRNKETREPM
                     AGFNSAAPEMQALGKYFRQEGLYTIVRWNTFFTNPPLCITEDELREAFSIIDKGLEIT
                     DQAII"
     gene            2492..3427
                     /locus_tag="VFH96_08875"
     CDS             2492..3427
                     /locus_tag="VFH96_08875"
                     /inference="COORDINATES: protein motif:HMM:NF006094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET6974122.1"
                     /translation="MNNVTELTREKIQATERVIRPYIRRTPILEVDGADFGLDSIKIT
                     FKLELLQHAGSFKARGAFTNILTREVPAAGVVAASGGNHGVAVAFAAMKLNQPATIFV
                     PTVASKTKLDRIRRYGAELVITGDRYADSLEASEAWTTQSGALPIHAYEGDETLMGQG
                     TLGMELEEQAPELDSVLVAVGGGGLIGGVASWYQNRVKVIAVEPSEAPTLQRALSAGR
                     PVDSPAGGIAADSLAPRQVGQQMFPIAQKYVHRALLVSDEEIIAAQKRLWETTRIIAE
                     PGGATAFAGLLSGRYKPEPGERVGVIVCGGNTDIL"
     gene            complement(3488..5875)
                     /locus_tag="VFH96_08880"
     CDS             complement(3488..5875)
                     /locus_tag="VFH96_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974123.1"
                     /translation="MPLGGKSQGNWSLSDISLKYRLPILIGTLLAAIILACSWASYRA
                     VKASALDVGRERLKNLTQQLSTTLQQSASNLLTKTVTVANEPVLRAFVKSPETAAQAD
                     VQKLLHQFTVSQDPNAIRVELWNAQGALLLVSPEADKKPLGDLTTEFKQVSSEPFKAV
                     GEIRQLQDVIGFPTVAAIKSEDGKVTGYLVRWRKLSASADTRQQMTRLLGTSATLYLG
                     NQNNETWTDLANIVAKPPVDVRGKEDISAYERNGERNGEVIGLARPIAGTPWLLLIEF
                     PDNVVLKPVSGFITRMVVIGLFLFVMGIAGAFVLSRNITGPLYALTGSATAISRGDDS
                     RTVDIHQDDEVGQLASAFNTMVEKLRDSQRELERKVQERTAQLEEANRQLESLSQTNA
                     HKRSIAEKERADALEALYNTEKQLLQSQKLEAVGRLAGGISHDFNNLLTVILGYSDIM
                     KRNLQQGHPLRRNVEEIVRASERAASLTRQLLAFSRKQVMQPKVFDLNTVISELEKML
                     RRMIGEDIELRVNLQDELDHIKADPVQLEQVIMNLVVNARDAMPRGGKLSIETNNVYL
                     DEAYAREHVSVVPGDYVMLAISDTGCGMSEETRLHIFEPFFTTKEQGKGTGLGLSMVY
                     GIVRQSGGNIWVYSEEGRGTTFKVYFPRVTAHAEEHKRTSGALDGPRGSETILLVEDA
                     ELVRNLARQVLETAGYRVLEAANAEAAIDLCETINGDRIDLLLTDVVMPGMSGNEMSR
                     ILVAKQPGMPVLYMSGYTDEAIVQHGMLEAGINFLQKPFTPGALASKVREVLDTR"
BASE COUNT         1306 a         1724 c         1717 g         1371 t
ORIGIN      
        1 agctctcgga acgaagcaag tcatacgaga acaattcaga cgaccagaag agcttcgacg
       61 aagacttcgt gagaattgtg cgttacctcg agagtgaagt tccgccgcag gtgcagggag
      121 tcgcgatctt cgcgtgctcg gctgacagcg actggtttgt agtcggactg tttgaggcgc
      181 cgttcgaacg caaccgcttg ttcgtttcgg atcgaccgca tctttatccg ctcgcacggc
      241 tggtcgatca atatcgacgt tatgcagtgg tgctcgccga cacgaatcgc gcgcagatct
      301 ttgtctttgc tgccggccgc gccgttgagc aggagcaggt agaaaatgtg aagacgaagc
      361 aaacgaaggt cggcggctgg tcacaggcgc gctaccagcg acacgtacag aattatcatc
      421 tccaacacgc gaaggaagtg gtcgatacgc tggagaagat cgtgcgcgac gagaatatcg
      481 agcacgtaat tctcgctggg gatgaagcgg tggtaatccc aatgctgcgc gagcagatgc
      541 caaaaacact cgaagaaaag atcattgacg cgctcagtct tggcatcgac acgcccgagc
      601 acgagttgct tgatgagtcg ttgactgcgt ttcaacgtca cgactcgctc actgacatgg
      661 agaaagttga gcgactactt aatgaatatc gtgctgatga tctcggggtg gcgggtgttt
      721 ccgagacgct agccgcccta tcaaatggcc aggtcgaaga gatgctgatc gcggcgaaag
      781 ccgagagtct tcagtatgac aaggaagaag tcgaaaaggt tttgaagctt tacgatgtcg
      841 atggagcgct gcctgaagag ctggaccagc gcacggttgc agatgagctg gtgcgacgag
      901 cgaatgtgtt gtcgtcggcg cgagtaacgt ttattgagga cagcacgcgg ctcgagcgag
      961 tcggtggtgt tggtgcgttt ctgcgctacc ggatctctga gccgcaggtg aacgcggata
     1021 aacgcggatc agatcagcgt ttatccgcgt caatccgcgg ctaaattatt gcttgatctg
     1081 tgatctcgag gcctttgtcg atgatcgaga aggcctcgcg cagctcgtct tccgtgatgc
     1141 agagcggcgg attggtgaag aaggtgttcc agcgcacaat cgtgtacaga ccttcctgac
     1201 gaaagtactt tccgagcgcc tgcatctcgg gcgcagcgct gttgaatcca gccatcggct
     1261 cacgcgtctc cttgttgcgc acgagctcaa caatcccgaa gagtccgatg tttcgtgtcg
     1321 caccgactga cgggtgtcgt tgctcgagat cttcgagtag cccgcgcatc acagttccca
     1381 ggcggcgcgc gttctctatc agattatcct cttcatagac ggcgatgttc gcgagggcgg
     1441 cggcgcatgc gagcgggtgg ctgttgtaag tcaatccgcc gtagaacaca ttcttctcaa
     1501 agtgatcggc aatctggcga cgcatgccga ccgcaccgag ttgcacatac gcgctcgaca
     1561 agcctttcgc catcgagatc agatccggaa caactttcca gtgatcgatc gagaaccact
     1621 ctccggtgcg accgaaacct gccatcactt catctgcgat cagcaggatg ccgtacttat
     1681 cgcaaagcgc acgcaggccc tgcatgtaac catccggcgg aatgagaatc ccattcgtgc
     1741 cgacaaccgg ctcgacgatc agtgcggcta tcgtgtttgg tccttccagt tcgagtactt
     1801 cttccgtgcg tgaaagcacg tcttcagtcg tgtcccaacc tttttggatg ccgtgataaa
     1861 aatcaggaat gcgaaacact ccggggatgc ctggctcagc cgcccagcgt cgcggatcgc
     1921 ccgtcagtgt caacgcgccc gccgtgccgc cgtgataagc gcgataacgc gcgccgatct
     1981 tgtggcgtcc ggtgtagagt ctggctatac gaatcgcatt ctcgttcgac tcggcgccgc
     2041 cgttcgtgaa gaagaacgcg tcgatgtcgc cgggtgtcag ctcggcaagc ttcacactca
     2101 ggcgtgcgcg cgtttcggtc gccatgaatg ggttggcgta acaaagcgtg tcagcctgtt
     2161 ctttgatcgc ccgaatcaca cgctcgtcgc cgtgaccgat gttgacgcac atgagctggc
     2221 tgttgaaatc gatgaagcgt ttgccttcgg gcgtccagaa ataaatgccc ttcgcgcgcg
     2281 cgacgggaat cggatcgaca gcagcctgag cagaccattc gaagagagtg tggtggcgac
     2341 agagctcgac gatctgctcg cctgtcattg tttcagcagt ggttgtcatg atgtttggga
     2401 tgctaacgaa atcagaagtg attgacaaac cgttatccac agattaggca gattgttata
     2461 tctgtgtaat ctgtggatac tcttcttaga tatgaacaac gtaaccgaac ttacgcgaga
     2521 aaaaatccag gcaaccgaac gtgtgatccg tccatacatc cgtcggactc caattctcga
     2581 agtcgacggc gcggattttg gactcgattc gatcaagatc acattcaaac tcgagctctt
     2641 acaacacgcc ggatcgttca aagcacgtgg cgcgttcacg aacatactga cgcgtgaagt
     2701 tccagcagcc ggcgtcgtgg cggcgtcagg cggcaatcac ggtgtggccg tcgctttcgc
     2761 ggcgatgaaa ctcaaccagc ccgccacgat cttcgtgccc acggtcgcat cgaaaacgaa
     2821 actcgatcgc attcgccggt acggagccga gctcgtgatc accggcgatc gctacgccga
     2881 ctcactcgaa gccagcgaag cctggaccac acaatcgggc gccctcccga ttcatgcgta
     2941 cgaaggcgat gaaacactga tgggccaggg cacactcggc atggaactgg aagaacaggc
     3001 gccggagctc gattcagtgc tcgttgctgt cggtggtggt ggattaatcg gcggcgttgc
     3061 gtcgtggtat caaaaccggg tcaaggtcat cgcagttgag ccaagcgaag caccgacctt
     3121 gcaacgtgcg ctgagcgccg gccgtccggt cgactcaccc gcaggcggta tcgccgctga
     3181 ctccctcgcg ccgcggcaag ttggtcaaca gatgtttccc atcgcgcaga aatatgttca
     3241 ccgcgcactg ctcgtctcgg atgaagagat cattgccgca cagaagcgac tgtgggaaac
     3301 aacgcgaatc atcgccgagc ccggcggcgc gacggcattc gccggacttc tatcaggacg
     3361 ctataaaccc gaaccaggcg agcgcgttgg ggtgattgtt tgtggtggaa atacagacat
     3421 cttgtaaatt ctgtaaatcc tgaaatcatg tctaacaaga tgacaggatt tgcaaaattg
     3481 acaggattta acgggtatca agcacctcac gcactttcga cgcgagcgca cccggcgtga
     3541 acggcttctg taaaaagtta atccctgctt ccagcatgcc gtgctgcacg atcgcctcat
     3601 ccgtgtagcc cgacatgtaa agcacaggca tacccggctg cttcgccacc agtatccgcg
     3661 acatttcgtt cccactcatg cccggcatca ccacgtcagt caacaacaga tcgatcctgt
     3721 cgccattgat cgtttcacac agatcgatcg cagcttcagc attcgccgct tctagcactc
     3781 gataaccagc agtctcgagc acttgtcgcg caaggttccg caccagctcc gcgtcttcaa
     3841 ctaacaggat cgtttccgag ccgcgtggcc catccaaagc tcctgaagta cgtttgtgtt
     3901 cttcagcatg cgccgtcact ctcggaaagt agaccttgaa tgtggttcct cttccctctt
     3961 cgctgtacac ccagatgttg ccgcccgact gcctgacgat gccgtaaacc atcgacaatc
     4021 ccaggccggt ccccttcccc tgttccttcg tcgtgaaaaa tggctcgaag atgtgcaggc
     4081 gcgtttcctc actcatgccg caaccggtat cgctgatcgc aagcatcacg tagtcacccg
     4141 ggaccactga aacgtgttcg cgcgcatacg cctcgtcgag atagacgttg ttcgtttcga
     4201 tcgataactt gccaccgcgc ggcatcgcat cccgagcgtt caccacgagg ttcatgatca
     4261 cctgttcgag ctgcacgggg tcggctttga tgtggtccaa ttcatcctgt agatttactc
     4321 gcaactcgat gtcttcgccg atcatacgcc gcaacatctt ttcgagctcg ctgatcactg
     4381 tgttcagatc aaacactttc ggctgcatca cctgtttgcg gctgaacgcc agcagttgtc
     4441 gcgtaagcga agcggcgcgc tcgctggccc tgacgatctc ctcgacattg cgtcgcagcg
     4501 gatgaccttg ctgcaagtta cgcttcatga tgtcgctgta gccgagaatc acggtcagca
     4561 ggttgttgaa gtcgtgcgag atgccgcccg cgagtcgacc aacggcttcg agtttttgcg
     4621 actggagcaa ctgcttctct gtgttgtaca aagcttcgag cgcgtcggcg cgctccttct
     4681 cagcgatgct gcgcttgtga gcatttgttt gcgacaaaga ttcaagctgc cggttggctt
     4741 cctcgagttg cgcggttcgt tcctggacct tgcgttcgag ctcgcgctgc gagtcgcgca
     4801 acttctccac catcgtgttg aatgcgctgg cgagttgccc gacttcgtcg tcctggtgaa
     4861 tgtcgacagt gcgcgagtca tcgccgcggc tgatcgcagt tgcgctaccg gttaacgcgt
     4921 acagcgggcc ggtgatgttt cgactgagca caaacgctcc cgcgattccc atcacgaata
     4981 aaaacaagcc gatcactacc atgcgcgtga tgaaaccact gacgggcttg agcaccacgt
     5041 tgtccggaaa ctcgatcaat aggagccaag gagtgccggc aatcggccgt gccaggccga
     5101 tcacttcgcc gttgcgttcg ccattacgtt cgtaggctga gatgtcttct tttccgcgaa
     5161 cgtcgaccgg cggttttgcg acgatgtttg cgaggtcggt ccacgtttcg ttgttctggt
     5221 tgccgagata gagcgtcgcg ctcgtgccga gcaatcgcgt catttgctgc cgcgtgtcgg
     5281 cgctggccga gagcttgcgc cagcggacca ggtagcccgt gacttttccg tcttcactct
     5341 tgatcgccgc gaccgttgga aaaccgatca cgtcttgcaa ctgccttatc tcaccgactg
     5401 ccttgaacgg ttcagacgag acttgtttga actcagtcgt tagatcgccg agtggttttt
     5461 tatcggcctc gggtgagacc agtaaaagcg cgccctgggc attccagagt tcgactctga
     5521 tggcgtttgg gtcttgcgat acggtgaact ggtgcaacag cttctgaacg tcagcttgcg
     5581 ctgcggtctc aggagacttc acgaacgcac gcagcacggg ctcgtttgca actgtgaccg
     5641 tcttcgtcag taagttgctg gccgactgct gcaacgtggt cgagagttgc tgcgtcaggt
     5701 tttttagacg ctcgcggccc acgtcgaggg cggaagcctt caccgcgcga tacgaggccc
     5761 aactgcaagc caggatgata gccgccagca gagtcccaat gagaatcggc agacgatact
     5821 ttaaggaaat atcggagagt gaccagttac cttgcgactt cccaccgagt ggcatcgaca
     5881 caccttccca tgacctaatc tgccgtcgaa acggctgggg gcttactgtg agtatctaac
     5941 tgggaggttg tcactcgcta aggcgagtaa cgggaggcaa gatcttctta cattgcagag
     6001 aataaaatca gtacgtttat aggtcaagag gtcaagagga gatttttgac ccaccttgca
     6061 ttgggccact caccgacaac tgtaggggtg gccctccgtg gccacccgtt gcttgttg
//
LOCUS       DASSFQ010000245        19426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1892551, whole genome shotgun sequence.
ACCESSION   DASSFQ010000245 DASSFQ010000000
VERSION     DASSFQ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19426
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892551"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..465)
                     /locus_tag="VFH96_08885"
     CDS             complement(43..465)
                     /locus_tag="VFH96_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6974124.1"
                     /translation="MLIRDVIRNREPYSTRATATVQEAAEFMAARNIGAVCVVDERGT
                     LLGVFSERDVLNRVVVAKRDPNNVKVGDVTSELRAVIQCDETPHQALERMELIGSRHL
                     PVVDGERWVGMLSMRDLLRVEVGEQGDEIKLLHEYIQH"
     gene            complement(533..1549)
                     /locus_tag="VFH96_08890"
     CDS             complement(533..1549)
                     /locus_tag="VFH96_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6974125.1"
                     /translation="MQYVENPPNPWLTHSVEWIGEPPQAKLEVFEETATRHIITHNNS
                     PDVGFDYTVNCYRGCIHGCTYCFSRPTHEYLGYGAGTDFDRKIVAKVNAPELLRKELM
                     RPSWQGAEIVFSFTSDPYIPLEANYKLTRRCLEVCAEFRNPVGVITKSALIRRDIDVL
                     QDLARNASLGVFFTIPFTDVETARAVEPYAPLPEARFHAMEALAAAGIRVGIGIAPVI
                     PGLSTDIPVLLKRAKEAGAESAFINMLRLPGNVAPYFEERLRAELPTKADRVLNRIRE
                     AREGKLNSSVFGERMRGKGQYWEATERLFKIHSQRLGFNKKRTHQKPAGSTFRRPTAQ
                     ASLF"
     gene            complement(1609..1818)
                     /locus_tag="VFH96_08895"
     CDS             complement(1609..1818)
                     /locus_tag="VFH96_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974126.1"
                     /translation="MAGQHIENLFRRFKGEVVDVKTVSGGIYSGRVVEVTNDYVCLTE
                     TSGAPGQQVFVTFGTIESMAVTAAD"
     gene            complement(1822..2175)
                     /locus_tag="VFH96_08900"
     CDS             complement(1822..2175)
                     /locus_tag="VFH96_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6974127.1"
                     /translation="MDLLFLEVNSLEESLAFYEGVLGMEVESVNNDSEPPMAKVRGGA
                     LRITLAQNLETMLRRGRGVHFFVGVGDVEQFYDDLIARGADVAAPVDEGWGGRFVTLE
                     DPDKYRYFFVTWKER"
     gene            complement(2184..2738)
                     /locus_tag="VFH96_08905"
     CDS             complement(2184..2738)
                     /locus_tag="VFH96_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET6974128.1"
                     /translation="MSQFDSNKDYYGVLGVDKHASQIEIERQFKRQASKHHPDRGGNE
                     EQMKSLNEAYRVLKDKSLRNSYDETQRRPVSHHAFTPVTTPAARDVGVLGHCLSAGLC
                     LIAGVFLLLLVRFQWIFFLWPLGLLALVVLGFGVLLARSAMVAVNDSLPVTNAFKRHT
                     KIQEVAFWTAIVSGGYGLYVLMTL"
     gene            2817..3485
                     /locus_tag="VFH96_08910"
     CDS             2817..3485
                     /locus_tag="VFH96_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974129.1"
                     /translation="MLKVENLSKEYSTPAGPLRIVSDVSLTLPPGSAVSIMGPSGSGK
                     STLLYMIGALEPPSSGTVTLKGRNPFLLNARELAAFRNQQIGFVFQDHCLLPQCSVIE
                     NVLTPTMVAAAANGEPTRRATELLKQVGLSERLDHRPAQLSGGEKQRAALARALIMKP
                     QLLLCDEPTGNLDQKSAEIVADLLLEIHQNAGTILIVVTHSKELAARFPQRFELKNQH
                     LESL"
     gene            3485..6832
                     /locus_tag="VFH96_08915"
     CDS             3485..6832
                     /locus_tag="VFH96_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6974130.1"
                     /translation="MQTTQLIKRSLAYYWQTNLVVVLGVAIAVSVLAGALLIGESVRG
                     SLRDLSARRLGKTDDLIAAPNFFREQLAADLESDIGATCPLIAIKGVVVHESSKRRVG
                     DVKIYGVDDRFWKFHGIGGVKTPDNRNVLVSQSLASELGSGPGESILLRIEKPSDIPI
                     ESLHGRKEDPGKTIRLSVSGVLGSESLGEFSLQPQHDAVRAVFVSLAFLQRELEQDVG
                     IGRINTILVAEHQKNAAALLKEKATLEDFGLKLRALDNQRGVSLESNSRIINEHVATA
                     ANNAAKALSIRTVPVLSYLANNINSGERSTPYSLVTAVDDATLAQIAKTSIQASQHPP
                     IILNDWTARDLDAKPGDTVSLEYYLWHENGRLETKKADFQLAAVVPISGLAADRDLVP
                     EYPGITESEDMSAWDPPFPIDLGRVRKEDEDYWHQYRTTPKAFIPLSTAQQLWQTRFG
                     KLTSIRLESGNQNYAETLRKALDPALMGFQIVPVREQGQSASSGATDFGEYFLYFSFF
                     LVVAALMLTTLFFKLGIEQRAREIGTLQALGFSDTGIRRLFLIEGTLLAGIGSLLGLV
                     GAIAYAALLMYGLRTWWVGAVGTTDLRLHVSWLWLVIGAVGGVLTSIVCIFSTLRGLK
                     RLSIRGLLAGDRTERGSAGSNFQSGAADQTLTRRYRARFRALLGILLTIAGIALLVLG
                     AVQLIPQAAGFFGGGVVLLVAAVWFISRWLKRPTRSYIHGAGWWTIARLGFRNATYHP
                     ARTVLCITLIASAAFIIVAVDSFRRSGVSAADRKSGTGGYPLLAESLLPIVHDPNTSE
                     GRESLNLNTDEATLKNLSFVNLRVRPGDDTSCLNLYQPRNPKIVAPPDSFLRDNRFTF
                     QTSIDKNDNPWLLLDRQFEDGAVPVIGDANSLTYVLHLKVGDEMTVDHANGPLKLRIV
                     AALSDSIFQSELLMSEKNFLRLFPSEEGYRMFLVDVPDKQQSAAVSTALEERLSDFGF
                     DAVSTEERLANFHRVENTYLSTFQMLGGLGLALGTLGMAAVLLRNVFERRRELALLRA
                     VGYNSSHFAAMVITENVLMLVLGLAVGFVCALLAIAPVLFERGGRLPNISLGLLLLAV
                     LLSGATASLVATLAALRSPLLPSLRAE"
     gene            6868..8106
                     /locus_tag="VFH96_08920"
     CDS             6868..8106
                     /locus_tag="VFH96_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6974131.1"
                     /translation="MRTTKWILASLLLFGIVADARTQSDWAQWGGPKRDFTTTSKGLA
                     ATWPATGPKQLWTRPLGAGHSAILASGNTLYTMYSAGDQEVVIALAADTGKTIWEYKY
                     DAPAAGMNYEAGVGPHSTPLLVDDMLYTVGATGKLFALDKKSGKVAWSHDLWKDFGGR
                     KIDRGYSCSPLAYKNTIILTLGGRENQTLIAFNQKDGSVAWKNVSLDMSPSSPILINV
                     DGQEQLVAFLGKVVAGIDPNNGNLLWSHPHVTEWGLNITMPVWGPDNLLFISSAYSGG
                     SRVLKLTQKDGKTTAEELWFHRRMRVHHGTAIRIGDYVYGSSGDFGPAFFAAVNVKTG
                     EIAYQDRTFPKSNFVLADGKLIILDEDGNLALATATPTALKVISKVSVLKNLAWTVPT
                     LVGTKLYLRDRRTITALDLS"
     gene            8179..9429
                     /locus_tag="VFH96_08925"
     CDS             8179..9429
                     /locus_tag="VFH96_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6974132.1"
                     /translation="MKRLGVVALLLVTFAIVRADNWPQWRGPNLNGVSNEKNLPVKWT
                     TDENVAWKVPMPGYSGSTPIIWRDRIFLNVADGDNLFLWCLNKTNGEVLWKKPLGDGN
                     VKMRKQNMSSPSPVTDGRSVYVMTGTGILKGFDFNGKELWTRDIQKEYGQFGLQWGYA
                     SSPLLHEDSLYVQVLHGMKTDDASYVMRIDKKTGKTLWKVDRPTNAIRESPDSYTTPA
                     LLRYGKTTEIVITGGDCVTGHDSATGKELWRANGLNPDNNPSYRIVASPIIFDNIIYA
                     PTRIKPLLALKAGGRGDITSSHVLWSTGNGPDVPTPVTDGKYFYVVNDRGIMFCLDAK
                     TGAEVYVQQRIKPGTYSASPVLADGKIYITNEDGLTTVVAAGPKFEVLAENPLNDYVL
                     SSPAISDGQIFIRTAAHLYAIGKK"
     gene            9475..11004
                     /locus_tag="VFH96_08930"
     CDS             9475..11004
                     /locus_tag="VFH96_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974133.1"
                     /translation="MKRFVVLVVMVGLAALSIRAQESQLGRVEFPTSGSKEAQAHFLR
                     GLAALHSFWYEEAVKAFRESTKVDPDFAMGYWGEAMTYNHPLWSEQDIAAARQVLTKI
                     KDSAKMTERERAYIAAVRLLYGEGDKPARDAAYSAQMEKVYRAYPEDLDAAAFYSLSL
                     LGMAGGGKGYRLQAKAGAIALEVYQKNPNHPGAAHYIIHAFDDPDHAILALPAARRYA
                     SIAPEAHHARHMPSHIFLQLGMWPEAAASNEAAWESSDAWMKRKNLSLSVRDYHSLHW
                     LLYAYLQQGRYNDAEKLLNLMKKVMSESTYDNKLRPGYYENNYANMAAAFVVETERWN
                     LATELFPESKPVSTTEQVSMNGSHSSHNAMQSGATLRTSSASVTLPFFIRGLAAAMNG
                     SPIEPSIAGLQAIHSNTSEIFVLEIGALDASMKKDHAKAIQLMKAATTLEESMTAPYG
                     PPSLIKPSHELFGEILLRAGKPADAAEQFKIALQRQPNRARSLLGATRAAEQLGSQTN
                     R"
     gene            complement(11067..12305)
                     /locus_tag="VFH96_08935"
     CDS             complement(11067..12305)
                     /locus_tag="VFH96_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6974134.1"
                     /translation="MKRRFLLPAAVALLSLALGLVAQAMAQTAAVWPQWRGPNRDGIS
                     KETGLLKQWPAEGPPLVWKASGAGSGYSSFSISDGKLYTMGLRGDREYVIAFDVATGK
                     EAWATAHGSAFRNDRGNGPRGTPTVDGDRVYALGGNGDLSALEARTGKIIWSKNVLKE
                     FGGSNIQWGISESPLVLGNKVLVNPGGPGASIVALNKADGSLIWKSQSDRAGYSSGIP
                     VEINGATQVVFFTSERAVGLDAKDGRLLWDYSRPSNNVANVATPIVRANRVFISSDYG
                     TGGGVVEIKPDNKAQEIWFTKDMRNHHSSSVLIGDYLYGFSSAILTAIKFDTGEIAWR
                     DRSVGKGSLVYADGNLYCFSENNVVGLVEATPTGYKEKGRFRIPQGQSPTWTHPVVAG
                     GRLYLRDQDTIYAFDVKEKK"
     gene            complement(12364..12819)
                     /locus_tag="VFH96_08940"
     CDS             complement(12364..12819)
                     /locus_tag="VFH96_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974135.1"
                     /translation="MQLWILFALGAALSWGLYGPALHGGQVALGNNPMRALLCVGVAY
                     FLIGVLVPLVTLSSQGQLNGFTTKGSVGAIIGGALGAAGAICIIYAFRSGGVPTYVMP
                     LVFAGAPLVNVLYSMWLHPPKTSPNPLLYLGFVLAAAGGGLVLYFKPSS"
     gene            13169..16288
                     /locus_tag="VFH96_08945"
     CDS             13169..16288
                     /locus_tag="VFH96_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6974136.1"
                     /translation="MPASLAAQTHRASVRGTIYDPAHAAIPGASVSVTRTETGEVRTT
                     TANRDGEYAITSLPAGNYELKVEVTGFESFVQKIELLVNQERRLNIDLRLSGPAIKYE
                     VLGEPVPLKKDSASLGTVIENRQITGLPLDGRNFYELSLLVPGAVPPAQGSAGSARGD
                     FAFSVNGAREDANNFLLDGVYNVDPKLNTFGVRPSVDAIREFEMLTSTYDASFGRNPG
                     AQVNVILNSGSNDFHGSAFEFHRNAALDARNFFAPASEPKPKYIRNQFGGALGGPIKH
                     DRTFFFADYEGTRSREGITRITNVPTAEERVGNFSHSVFGVPTNPFTGMPFDNGVIPD
                     FFINPVGRAIAALYPLPNRNVPFQNFVSSPVLRDDNDSFDVRVDHRLTDRADLTFRYS
                     FGDRDLFEPFTGPTFSVVPGFGDTVKRRSQNGMAALTLVLTPNLVNESRVAFNRVAAS
                     VNQEASVLNSEVGLPTISSRERDLGLSFITITGFSPLGDEGNNPQDSVTNVYQFLNNS
                     SYVHGNHLFKFGADLRFSQQNAFRDVESRGRLQFSPFFQFTGNALSDMLLGFPLLTSV
                     ARVDNPQRIRTESYNFFINDSYRVTPRLTLNAGLRYEYNSPPVDADDRATIYDLVTRS
                     LVPVGTNGVPRSGFEADKNNFAPRVGFAWSFNEKTVLRGGYGIYYDQSPLAPAEALYF
                     NSPFFDNNIFFSLPGLPLTLNDPFPSFFPFPLPDSALAIQRDLRTGYMQHWNFNVERQ
                     IFSGSVLEVAYVGSKGTKLLTARDINQPQPSVLPPGLPFVPRPDPRFDDIDLLESRAN
                     SNYNALQVRYQQRLTRGFTALASYTWSKSIDDASNFFTSAGDPNFPQNSYNVAAERGL
                     SNFDVRHRMSLSYSYALPFRKPGDGLVANLVNGWETFGIVTLQSGRPFTVALLSEIDN
                     SGTGRSILGFGANDRPNVVGNPELSNGTTDQWFNTAAFSFPAPGTFGNAGRNILEGPG
                     FQNVNMSILKNTRLNERVNLQFRAEAFNLFNHPNFNLPDNFLGSPTFGRITSARDPRH
                     IQFGLKLLF"
     gene            complement(16376..17725)
                     /locus_tag="VFH96_08950"
     CDS             complement(16376..17725)
                     /locus_tag="VFH96_08950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6974137.1"
                     /translation="MNTNNNFDVIVMGGGPAGSTVAGILAREGRKVILFEKEIFPRHH
                     IGESLMTDTYWTFRRLGILEKMKESPFVRKYSVQFANSAGKESRPFYFFEAVHHESAV
                     TWQITRAQFDHLLIDHAAEQGVTVHQGVLVKQVLFEGDQAVGVEVQMPDGTREKYFAK
                     VVVDATGQMAMLSNKFRWRVRDPKLKKAVIYSYFKDAHREPDLNGGATLVLRTEAGSG
                     GWFWYIPLENDITSVGIVADPEYLVQGRGQDLAKIFQEEIDKCEPVRKRVAPGTRVDK
                     IYSILDYSYRSKHNAGNGFIIIGDAYGFLDPIYSSGVLLALKMAELAADAIHDAFNHD
                     DFSAARLGQYQAKLDRGIESMRKLVYAFYNDGFSFSGFLRKYPEERVHIINLLIGDVF
                     REGVDSIYGPMSEFAEIPPPLYEQVDGTMVQQTPSAEESDLLLSAQYRYYDDRPQTS"
     gene            complement(17722..19035)
                     /locus_tag="VFH96_08955"
     CDS             complement(17722..19035)
                     /locus_tag="VFH96_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HET6974138.1"
                     /translation="MTIDTASTGTEVGNYFVSNYPPFSQWKPEFISEAIDALNQPPRV
                     DDPLGLYIHIPFCRKRCLFCYFKVYTDKNASEIEVYLDALIKENELYSRSPGFQGRKL
                     RFAYFGGGTPSYISEKQLRYLVDGLNRHVSWQNAEEVTFECEPGTIQKAKLETLKAIG
                     VTRLSLGIEHFDDEILAANGRAHLSPEIYRAYQWAREVDFPQINIDLIAGMSGETEEK
                     WREAVRRAIELQPDSVTIYQMELPYNTVISQDILKKGAASPIAGWDTKRRWLNYAFEQ
                     FLAHDYEIAGTDIVATKKKTCRFIYRDALWHGGDMIGIGVSSFSHFGGVNFQNAHNFE
                     EYVRILNEDRLPLLRAVSLTPKQRLIREMVLQLKTGSIDTGYFQKKYGVDVWNEFRAV
                     YEELEEKHMLERHNSHVALTRDGLLQVDHFLPEFFEPEMRTVRYA"
BASE COUNT         4338 a         5512 c         5261 g         4315 t
ORIGIN      
        1 agagggccac ccctacaaaa gcacctccgc gttgttgata gactaatgtt gaatgtactc
       61 gtgtagtaat ttgatttcgt ccccctgttc gccaacttca acacgcagca gatcgcgcat
      121 cgagagcatg cccacccagc gctcgccgtc gacaacgggc aagtgtcgac tgccaatcag
      181 ttccatgcgt tcgagcgcct ggtgcggcgt ctcgtcacac tgaatcacgg cgcggagctc
      241 gctggtaaca tcgccgacct tcacgttgtt gggatcacgc ttcgcgacga cgacgcgatt
      301 gagcacgtcg cgttccgaaa acacacccag caacgtgccg cgttcgtcga cgacgcagac
      361 ggcgccgata tttctcgcgg ccatgaactc cgcagcttcc tgcacggtag ccgtcgccct
      421 cgtagagtaa ggctcacggt tccggatcac gtcccggata agcatcgctt tagcctcctg
      481 attctgttgt ggggtatggg tattatagag aatgcatgga ctgctgtctc tattaaaaca
      541 gacttgcttg cgccgttggg cggcggaagg tcgagccggc gggcttttga tgcgtgcgct
      601 tcttattgaa accgagacgc tggctgtgaa ttttgaagag acgctcagtg gcttcccagt
      661 attgaccttt gccacgcatg cgctcgccga acacactcga gttgagcttg ccttcacgcg
      721 cttcacgaat gcgattcaaa acacggtcgg ctttcgtcgg caactccgca cgcaaccgct
      781 cttcgaagta gggcgcaacg tttccaggca agcgcagcat gttgatgaac gcagactccg
      841 cgcccgcttc tttcgctcgc ttcaacaaca ccggaatgtc tgtcgacaga ccgggaatca
      901 ccggcgcgat accgatgccc actctgattc ccgccgcagc gagcgcttcc atcgcgtgaa
      961 atctcgcttc gggaagcggc gcgtacggtt ccactgcccg cgccgtttca acatccgtga
     1021 acggaatcgt gaagaacacg ccgagactcg cgttgcgcgc gagatcctga agcacgtcga
     1081 tgtctctgcg aatcaacgcc gacttcgtga tcacacccac cggattgcga aactctgcac
     1141 acacttccaa acagcgccgc gtcaatttgt aattagcttc gagcggaatg tacggatcgg
     1201 aagtgaacga gaaaacgatc tcggcgcctt gccacgatgg ccgcatcagc tctttgcgca
     1261 gcagctcggg cgcgttcact ttcgcaacga tcttgcgatc gaaatcagtc cccgcgccat
     1321 agccgaggta ctcgtgcgtc gggcggctga agcaataagt gcagccgtgt atgcagccgc
     1381 gatagcagtt gacggtgtaa tcgaaaccga cgtcgggact gttgttgtga gtgatgatgt
     1441 ggcgcgtagc cgtctcttca aagacttcga gcttcgcttg cggtggttca ccaatccatt
     1501 cgactgagtg cgttaaccac ggattcggcg gattttctac gtactgcata aagcttaaaa
     1561 cctagccgcg gatttgcgcg gataaacgca gatttgcgtt catccgcgtt aatccgcggc
     1621 tgtaactgcc attgattcga tcgtgccaaa tgtaacgaac acctgctgcc cgggggcgcc
     1681 ggaagtctcc gtcaaacaca cataatcatt cgtgacctcg actacgcgtc cggaatagat
     1741 cccgcccgac acagtcttca cgtccactac ttctcccttg aagcggcgaa aaagattctc
     1801 aatgtgttgt cccgccatcc gtcacctttc tttccacgtc acaaagaagt agcgatattt
     1861 atcaggatcc tcgagcgtaa caaagcgtcc gccccagcct tcatcaaccg gcgccgcgac
     1921 gtccgctcca cgtgcgatca aatcatcgta aaactgctcg acatctccaa ctccgacgaa
     1981 gaagtgtact ccacgcccgc gtcgcagcat cgtttcgagg ttttgcgcca gcgttattcg
     2041 caacgcgcca ccacgaacct tcgccatcgg aggctccgag tcattattga ctgactcgac
     2101 ctccatcccc agcacacctt cataaaacgc cagcgactcc tcaagactgt tgacctcgag
     2161 gaaaagtaaa tccaaaccag tgatcataac gtcatcaaaa catacagccc gtaaccgccg
     2221 ctgacaatcg ccgtccaaaa agcgacctcc tggatcttcg tgtgacgctt gaacgcattg
     2281 gtcaccggta gcgaatcgtt aactgcgacc atcgcgctgc gcgcgagcag cacaccgaat
     2341 ccaagcacca cgagggcgag aagtccgagc ggccacaaaa aaaagatcca ttgaaatcgc
     2401 accagcaaca acagaaacac gcctgcgatc aaacaaagcc ccgcgctcag gcagtgacct
     2461 aaaactccga cgtcccgggc ggccggcgtc gtgaccggag tgaatgcgtg atgactgacc
     2521 ggtctacgtt gcgtctcatc gtacgagttg cgcagcgact tgtctttgag aacgcggtaa
     2581 gcctcgttca aagacttcat ctgttcctcg ttgccgccgc gatccggatg atgcttcgac
     2641 gcttgtcgtt tgaactgccg ctcgatctcg atctgagagg catgcttgtc gacgcccaaa
     2701 acaccgtagt agtctttgtt cgaatcaaac tggctcatcg cgaatttcct gattctagaa
     2761 gggtgatatt tgtttggcaa acttaagccg cggatgaacg cggattaacg cagattatgt
     2821 taaaggttga gaatctctcg aaggaatatt ccactcccgc aggaccactg cgtatcgtct
     2881 ccgacgtgtc gctaacacta ccgcccggct cggcagtttc gatcatggga ccatcgggca
     2941 gcggcaagag cacgctgctc tacatgatcg gcgcgctcga gccgccgtcg agcggcacgg
     3001 tcacattaaa aggacgaaac ccgttcctcc tgaacgcaag agagctcgct gcgtttcgca
     3061 atcaacagat cggttttgtg tttcaggatc actgcctgtt gccgcaatgt tcggtgatcg
     3121 agaacgtgct gacaccgacg atggtcgccg cggctgcgaa tggagaaccg acacgccgcg
     3181 ccaccgagtt gctcaaacaa gtcggactca gtgaacgcct cgaccatcgt ccggcgcaac
     3241 tctcgggcgg cgagaaacaa cgtgcggcac tcgcgcgcgc gctgatcatg aagccgcaac
     3301 tgttgttgtg cgacgagcca accggaaatc tcgatcaaaa atcggctgag atcgttgctg
     3361 atttactgct cgagattcat cagaatgccg ggacgatctt gatcgtcgta acgcatagca
     3421 aggaactcgc tgctcgtttc ccccagcgtt tcgaattgaa gaaccagcat ctcgaatcgc
     3481 tttgatgcag actacccaac tcataaaacg cagtctcgcg tactactggc agacgaatct
     3541 cgtggtcgtg ctgggtgtcg cgatcgctgt ttccgtactc gcaggcgcgc tgctgatcgg
     3601 tgagtcagtg cgcggtagtc tgcgagatct ttccgcgcga cgcctcggca agacggacga
     3661 tctgattgcc gcgccaaact tcttccgcga acaactcgct gcggatctcg agagcgacat
     3721 cggagcgacg tgcccgttga tcgcgatcaa gggcgtggtg gtccatgagt cgagcaagcg
     3781 acgagtcggt gatgtgaaga tttacggcgt tgatgatcgg ttctggaagt tccatggcat
     3841 cggtggtgtg aagacgccgg acaatcggaa cgtgctcgtg agtcaaagcc tggcgagtga
     3901 gctcgggagt ggtccgggtg agtcgatact gctgcggatc gagaagcctt ccgatattcc
     3961 gatcgagtcg ttgcacggac gcaaggaaga tccggggaag acaatcaggc taagtgtgag
     4021 tggggtgctt ggtagtgaat cgctgggaga gttttcactg caaccacagc atgacgcggt
     4081 gcgtgctgtg tttgtgtcgc tcgcgttttt gcagagagag ttggagcagg atgtcgggat
     4141 tggcaggatt aacacgatac ttgttgccga gcaccagaag aacgctgcgg cgctattgaa
     4201 agaaaaggcg acgctcgagg actttgggct gaagctgcga gctctcgata accagcgcgg
     4261 tgtttccctc gagagtaata gccgaatcat caatgaacac gtggcgacag cagcgaacaa
     4321 tgctgccaaa gcgctctcga ttcgaaccgt gccggtgctt tcgtatctgg ccaacaacat
     4381 caactctggc gagcgctcga cgccgtattc gctcgtgact gcagtcgatg acgcgacgct
     4441 cgcgcagatt gcaaaaacct ctatccaggc ttcgcaacac ccgccgatca ttctcaacga
     4501 ttggaccgcg cgcgatctcg atgcaaagcc tggtgacacg gtttccctcg aatattatct
     4561 ctggcatgaa aacggcaggc tcgaaacgaa gaaggctgac tttcagttag ccgcagtggt
     4621 gccgatttca ggattggctg cggatcgcga tctggttcct gagtatccgg ggatcactga
     4681 atcggaagac atgtcggcgt gggatccacc gtttccgatc gatctcgggc gagtgcgaaa
     4741 ggaagatgaa gactactggc accagtatcg caccactccg aaagcattca tcccgctctc
     4801 aactgcacag caactctggc agacgcgttt cggcaagctc acctcgattc gtttggagtc
     4861 tggtaatcaa aattacgccg agactcttcg caaagcttta gatccggcat tgatgggatt
     4921 ccagatcgtt cccgtgcgcg agcaggggca gtcggcttca agtggcgcga ctgattttgg
     4981 tgaatacttt ttgtatttca gtttctttct cgtcgtggcg gcgctgatgc tgacgacgct
     5041 ctttttcaaa ctcggaatcg agcagcgcgc gcgtgagatc ggaacgctgc aggcgctggg
     5101 gttttcagat accgggatac gacgactgtt tttgattgag gggacgctgc tggcagggat
     5161 tgggagtttg ttgggtttgg ttggagcaat tgcgtatgca gcgttgttga tgtacgggct
     5221 gagaacgtgg tgggttggag cggttgggac gacggacttg cggttacacg tgtcgtggtt
     5281 gtggttggtg attggtgcgg ttggaggtgt gctcacttcg atcgtgtgca tcttctctac
     5341 gttgcgaggc ctcaagcgtt tatctatacg cggtctgctc gccggagaca gaaccgagcg
     5401 cggtagcgcc gggtcaaact ttcaatcagg cgccgccgac caaacgttga cccggcgcta
     5461 ccgcgctcgg ttccgtgctt tgctcggcat tcttttgacc atcgcgggaa tcgcgctgct
     5521 cgtcctcggg gcagtgcaac taatcccgca ggcggccggc ttcttcggcg gtggtgttgt
     5581 attgctcgtt gcggcggtct ggttcatctc acgctggctg aaacgaccca ctcgatcgta
     5641 cattcacggc gccggttggt ggaccatcgc gcggctcggc tttcgcaacg cgacgtatca
     5701 tccagcgaga accgtcctct gcatcacact aattgcctcc gccgccttca tcatcgtcgc
     5761 ggtcgactcg ttccgccgct ccggtgtctc tgctgccgat cgtaagtcag gcacgggcgg
     5821 ataccctctg ctcgctgaat cgctgctacc aattgtccac gatccaaaca cgagcgaagg
     5881 ccgtgaatca ctcaacctga acaccgacga agccacattg aagaacctga gcttcgtgaa
     5941 cctgcgcgtc cggccgggcg acgacacgag ctgcctcaat ctatatcaac ctcgcaatcc
     6001 gaaaatcgtc gcgccccccg attcgttcct gcgtgacaac cgcttcacat ttcaaacctc
     6061 gattgacaaa aacgacaacc cgtggctcct cctcgatcgc caattcgaag acggcgcagt
     6121 cccagtcatc ggcgacgcga actctttaac ctacgtactg cacctgaaag tcggtgacga
     6181 gatgactgtg gaccacgcga atggtccatt gaagctccgc atcgtcgcgg ctctgtctga
     6241 cagcatcttt cagagcgagt tgttgatgtc cgaaaaaaac ttcctgcgtc tttttccctc
     6301 tgaagaaggc taccggatgt ttctcgtcga cgttccggac aaacaacagt ccgctgccgt
     6361 atcgacggcg ctcgaagagc gcctgtcaga cttcggcttt gacgcagtct cgacagaaga
     6421 acgccttgcc aactttcatc gcgtcgagaa cacatacctc tcgacatttc agatgctggg
     6481 cggtctcggt ctcgcactcg gcacactcgg aatggccgcg gtgttgctcc gcaacgtctt
     6541 cgaacgacgg agagagctag ccctcttgcg tgcggtgggg tataactctt cacacttcgc
     6601 cgcaatggtg attacggaaa acgttctgat gctggtgttg ggtctggcgg tgggttttgt
     6661 atgcgcgctg ctcgcgattg cgcccgtgct cttcgagcgt ggaggccgac ttccaaacat
     6721 ctcgctcggc ctcttgctac tagcagtctt gctttcgggc gcaaccgcat cgctcgtggc
     6781 cacactcgct gccttgcgct caccattgct gccgtcatta cgggccgagt aaatctgtgt
     6841 aatctgtgga taaaaaagga gcaaaccatg cgaaccacaa agtggatttt ggcttccctt
     6901 ctcctcttcg gaatcgtcgc agatgcacgc acccaatctg actgggcgca gtggggtgga
     6961 ccaaagcgcg acttcaccac cacctcaaaa ggcctcgcgg ctacatggcc cgcaactggc
     7021 ccaaaacaac tctggacccg gccactcggc gcgggccact ctgcgatcct cgcaagtggt
     7081 aacacgctct atacgatgta cagcgccggc gatcaggaag ttgtcatcgc gctcgctgcc
     7141 gacaccggca aaacgatctg ggaatataag tacgacgcgc cggcagcggg catgaattac
     7201 gaggccggag ttggaccaca ctcgacaccg ctgctcgtcg acgacatgct ctacaccgtc
     7261 ggcgccactg gcaaactctt cgcgctcgat aaaaaatccg gcaaggtcgc gtggtcacac
     7321 gatctctgga aagactttgg cggccgtaag atagatcgtg gctactcgtg cagtccactc
     7381 gcttacaaaa acacgatcat cctcacactt ggcggtcgtg aaaaccaaac cctgatcgcg
     7441 ttcaatcaga aagacggaag cgtagcgtgg aagaatgtgt cgctcgacat gtcgccgtcg
     7501 tcgccgatcc tcatcaacgt cgatggacag gaacagctgg ttgcgttcct cggcaaggta
     7561 gtcgcgggta tcgatccaaa caacggcaac ttgctctgga gtcatccgca tgtcaccgaa
     7621 tggggactga acatcacgat gccggtctgg ggcccggata atctgctgtt catctcgtct
     7681 gcttacagcg gcggcagccg cgtgctgaag ttgacgcaga aagatggaaa gacgacggct
     7741 gaagagctct ggtttcatcg tcgcatgcgc gtgcatcacg gcactgccat ccgcatcgga
     7801 gattatgtct acggatcgag cggcgacttt ggaccagcat tctttgcggc agtgaatgtg
     7861 aaaaccggcg agattgcata ccaggaccgc acgtttccga aaagcaattt cgtcctcgcc
     7921 gatgggaagt tgattatttt ggacgaagat ggaaatctgg cgctcgcaac cgccacacca
     7981 acagcattga aagtaatctc gaaggtaagt gttttgaaaa atctcgcctg gactgtgccg
     8041 acgcttgtcg ggacaaagct ttacttgcgc gatcgccgca cgatcaccgc gctcgatctt
     8101 agttgatcca cagattccgc agattgcaca gaaaatctgt gtagtctgtg taatctgtgg
     8161 ttaaaaacgg aggaatagat gaaacgtttg ggagtcgtgg ctcttttgct tgtgactttt
     8221 gcgatcgtcc gcgccgataa ctggccgcag tggcgtggtc caaatctcaa cggcgtcagc
     8281 aacgaaaaga acctgcccgt caaatggact accgatgaga acgtcgcctg gaaagtcccg
     8341 atgccggggt acagcggctc gacgcccatc atctggcgcg atcgcatttt cctgaacgtg
     8401 gccgacggcg acaatctctt cctctggtgt ctcaacaaaa ctaacggcga agtcctttgg
     8461 aagaaaccgc tcggcgatgg caacgtgaag atgcgcaagc aaaacatgtc gtcgccgtca
     8521 ccggtcactg acggccgcag cgtctacgta atgaccggca ccggcatact caagggcttc
     8581 gacttcaacg gcaaggagct ttggaccagg gacattcaga aagagtacgg ccagtttggc
     8641 ttgcaatggg gctacgcttc ttcaccgctg ctgcacgaag actcgcttta cgttcaagtg
     8701 ctgcacggca tgaagactga cgatgcgtcg tacgtgatgc gcatcgacaa gaaaaccggc
     8761 aagacgttgt ggaaagtcga tcggccgacg aatgcgattc gcgagtcgcc tgattcttac
     8821 accaccccgg cgctgcttcg ttacggcaag acaacggaga tcgtgatcac gggtggcgat
     8881 tgtgttaccg gtcacgattc ggcaacgggc aaagagcttt ggcgggcgaa tggtctcaat
     8941 ccggataaca atccttcgta tcgcatcgtc gcgtcgccga tcatttttga caacatcatt
     9001 tacgcgccga cgcgcatcaa gccgttgctg gcgttgaaag cgggcgggcg tggagacatc
     9061 accagctcgc atgtgctgtg gtccaccggc aacgggcctg atgtgccgac gccggtcact
     9121 gatggcaagt atttttatgt cgtgaacgat cgcgggatca tgttttgtct cgacgcgaag
     9181 actggcgctg aggtttacgt gcagcaacgc atcaagccgg ggacgtacag cgcctcgccg
     9241 gtgctggctg atgggaagat ctacatcacg aatgaggatg gactgacgac ggtggtggct
     9301 gccggaccga agtttgaagt gctggctgag aatccgttga atgattacgt gctcagctca
     9361 cccgcgattt cggatgggca gatcttcatt cgcactgcgg cgcatcttta tgcgatcggg
     9421 aagaagtaac cacagatttt cacggatttc atacacggat caggaaggtt aatcatgaag
     9481 aggttcgtag ttctggttgt catggttggg ctcgctgcgt tgagtatcag ggcgcaggag
     9541 tcgcagctcg ggcgggttga gtttccgacg tcgggatcga aggaagcaca ggcgcacttt
     9601 ctgcgcggac tcgcggcgtt gcactcgttc tggtacgagg aggcggtcaa ggcttttcgt
     9661 gaatcgacca aagtcgaccc ggatttcgcg atgggctact ggggcgaagc gatgacctac
     9721 aatcacccgc tctggtccga acaggatatc gccgcagcgc gccaggtact cacgaagatc
     9781 aaagactcag cgaagatgac tgagcgcgaa cgagcttaca ttgccgctgt tcgcttgctc
     9841 tacggcgaag gtgacaagcc tgcgcgcgac gcggcgtact cggcacagat ggaaaaggtc
     9901 tatcgcgcgt atccggaaga tctggacgcg gcggcgtttt actcgttgtc actgcttggt
     9961 atggccggtg gcggaaaagg ttatcgcttg caggccaaag ccggcgcgat tgcgctcgag
    10021 gtctaccaga agaatccaaa ccatccggga gcggcgcact acatcattca tgcgtttgac
    10081 gatccggacc acgctattct cgcgctcccg gctgcgcgtc gttacgcgag catcgcgccg
    10141 gaggcacatc acgcgcgtca catgccatcg cacatttttc tccaactcgg aatgtggccc
    10201 gaagccgcag cgtcgaatga agctgcgtgg gagtcgtctg atgcgtggat gaagcgcaaa
    10261 aacctttcgc tcagcgtgcg cgattatcac agcctgcact ggctgctcta cgcgtacctg
    10321 caacagggcc gctataacga tgcggagaag ttgctcaacc tcatgaagaa ggtgatgtca
    10381 gagtcgactt acgacaacaa gctgcgaccc ggctactacg agaacaacta cgcgaacatg
    10441 gctgcggctt ttgtggtcga gactgagcga tggaatcttg ccactgagct ctttcctgaa
    10501 agtaaaccgg tgagtactac cgagcaagtg tcgatgaatg gtagtcacag tagtcacaac
    10561 gcgatgcaga gtggagcaac gttgcgcacg tcgtcagcga gcgtgacact gccgttcttt
    10621 attcggggtc tcgcggcggc gatgaatggt tctccgatag aaccgagcat cgcagggttg
    10681 caggctattc attcaaacac ttcggagatt ttcgtgctcg agatcggagc gctagatgct
    10741 tcgatgaaga aggatcacgc gaaagcgatt cagttgatga aggcggcgac gacactggaa
    10801 gaatccatga ccgcacccta cggtccgccg agccttatca aaccatcaca cgagctcttt
    10861 ggtgagatcc ttttgcgtgc aggaaagcca gcggacgctg cggagcagtt caagatcgca
    10921 ctgcaacgac aaccgaaccg cgcgcgttcc ttactcggcg ccacccgtgc cgcggaacaa
    10981 cttggctctc aaaccaaccg ctaggtacac aaaaggcaca aaaagcacaa aagaattatt
    11041 tttgtgcatt ttgtgccttt tgtgtactac ttcttttctt tgacgtcgaa ggcgtaaatg
    11101 gtgtcttgat cgcgtagata caaacgtcca cctgcaacga ccgggtgcgt ccacgtcggc
    11161 gactggcctt gtggaatacg gaagcgtccc ttctccttat aaccagtcgg cgtggcctca
    11221 accaatccca ctacgttatt ctcgctgaag caatacaaat tcccatcagc atagaccagc
    11281 gatccctttc ctacactccg atcgcgccac gcaatctcgc cggtatcaaa cttgatcgcc
    11341 gtcagaatcg cactcgaaaa cccgtacagg taatcgccaa tcaacaccga gctgctgtga
    11401 tgattgcgca tgtctttcgt aaaccatatt tcctgcgctt tgttgtcagg cttgatctca
    11461 accaccccac cgccagtccc gtaatccgac gagataaaca cgcggttcgc acgcacgatc
    11521 ggcgtcgcga cattcgcgac gttgtttgac ggtcgcgaat aatcccacaa caaacgaccg
    11581 tccttcgcat ccagcccgac tgcgcgttca ctcgtgaaga aaacaacctg ggtggcgccg
    11641 tttatctcga ccggaatacc cgacgagtaa cccgcacgat cactctggct cttccagatc
    11701 aacgatccgt cagctttgtt gagcgctacg atcgacgcac ccggcccacc cggattcacc
    11761 agcactttgt tcccgagcac gagcggcgac tcgctgatgc cccactggat attcgagccg
    11821 ccaaattcct tcagcacgtt cttagaccag ataatcttcc cggtgcgtgc ctcaagcgca
    11881 gacagatcgc cgttaccgcc gagcgcgtaa actcgatcgc catcgactgt cggcgtgcct
    11941 ctcggaccat tgccgcggtc gttgcgaaaa gccgagccgt gcgcagtggc ccatgcttct
    12001 tttccagtcg caacgtcaaa cgcgatgacg tactcgcgat cgccgcgcag acccatcgtg
    12061 tacaacttgc cgtctgagat ggagaacgac gagtagccgc ttcccgcacc cgacgctttc
    12121 cagaccagtg gcgggccttc agccggccac tgcttgagta gccctgtttc tttcgagatg
    12181 ccgtcgcgat ttggaccacg ccactgcggc cagacagcag ccgtctgcgc catcgcctgc
    12241 gccaccaaac ccaaagccaa actcagtaac gcaaccgctg ccggcaacaa aaacctgcgt
    12301 ttcattctga tctccttcgt gtggaaaaaa ctcttgaatc cggggagaca cgaagaggcc
    12361 gctttaagac gagggcttga agtacaacac caggccaccg cctgcagcag ctaacacaaa
    12421 tcccagatag agcaacggat ttggcgatgt cttgggcgga tgcagccaca tcgaatacaa
    12481 cacattcacg agcggcgctc ccgcgaaaac gagcggcatg acataagtcg gtacgccgcc
    12541 tgatctgaac gcgtatatga tgcaaatggc gcccgctgca cctaacgctc cgccgataat
    12601 ggcgccaacg gagcccttcg ttgtgaagcc gttaagctgg ccctgcgaag aaagcgtgac
    12661 gagcggcacc agcacgccaa tcaggaagta agcgacaccc acacacaaca gggcgcgcat
    12721 gggattgtta ccaagtgcta cctggccgcc gtgcagagca gggccataca agccccacga
    12781 aagcgcagcg ccgagagcaa aaaggatcca aagctgcatc aggtcctcca gaatgagaga
    12841 tgatcacaaa agcacaaagc tgaatccaac aaacagcaca aaaaagcagc agacagtttc
    12901 tactctttct tttatgagcc ttatgtgccc tttcgtggct aaactcttgc gcctttttgt
    12961 ggctaaagct gaaacacgaa acaggctttc gccgtaatgt cttcgttcct aaaagttccg
    13021 cgcattctaa catcagtggc ttagacataa tagcaactca tttgctaagg tctcgcaaac
    13081 acgcggccgt ccaaaatctc gaacgaggag cgccaatatt ttgaagtcga gctttcaaac
    13141 cctctgtcta atagctctgt cactactcgt gcctgcttcg ctggcggcgc aaacgcatcg
    13201 cgcctcagtg cgagggacga tttacgatcc ggcacacgcg gcgatccctg gcgcaagcgt
    13261 ctcggtgacg agaaccgaaa caggtgaggt ccgcaccact accgcaaata gagatggcga
    13321 gtatgcaatt acttcacttc ctgcgggtaa ttacgagctc aaggtcgagg tgacaggttt
    13381 cgagagtttc gtgcaaaaga ttgaactcct tgttaaccag gaacgacggc tgaatatcga
    13441 tcttcgtctt tctggaccag cgatcaagta tgaggtttta ggcgagccgg ttccgctgaa
    13501 aaaagactcc gcttcactcg gcaccgtgat cgagaaccgt cagataaccg gactgccgct
    13561 cgatggtcgt aacttctacg agctcagcct gctcgtaccc ggcgccgtgc cgcctgcaca
    13621 aggctcggcc ggatctgctc gcggcgactt cgcgttcagt gttaacggcg cgcgcgaaga
    13681 tgcaaacaac ttcctgctcg acggtgttta taacgtcgat cccaagctca acacattcgg
    13741 cgtgcgaccg tcggtcgatg cgatccgaga gtttgagatg ttgaccagca cgtatgatgc
    13801 gtcgttcggt cgcaatccgg gcgcacaggt aaacgtgatc ctgaattccg gatcgaatga
    13861 tttccatggt tcggcattcg agtttcatcg caacgccgca ctcgacgcgc gcaacttctt
    13921 cgcacccgcg tcagaaccaa aaccaaagta cattcgcaac cagtttggcg gtgcgcttgg
    13981 tggtccaatc aaacacgatc gcacgttctt ctttgctgat tacgaaggca cacgttcgcg
    14041 cgaaggcatc acgcgcatca cgaacgtgcc gactgccgaa gagcgcgtgg gcaacttctc
    14101 gcacagtgtg ttcggagttc cgaccaatcc ctttacgggt atgccgttcg acaatggtgt
    14161 gattccggat ttctttatca atccggttgg ccgtgcgatc gcggcgctct atccgctgcc
    14221 aaaccgcaac gttccgtttc aaaacttcgt ctcatcgccg gtcctgcgtg acgataacga
    14281 cagcttcgac gtgcgcgtcg atcaccggct gactgaccgc gccgatctca cgttccgata
    14341 cagttttggc gaccgcgatt tgtttgagcc atttactggt ccaaccttct cggtcgttcc
    14401 gggttttggc gatacagtga aacgtcgcag tcaaaacggt atggccgcgc tgacactcgt
    14461 gctgacgccg aatctcgtga atgaaagtcg cgtcgcgttt aatcgcgtcg ctgcatcggt
    14521 caaccaggaa gcgagtgttc tgaattccga ggtcggtctg ccgacgattt catcgcgcga
    14581 gcgtgacctt ggactcagct tcatcaccat caccggtttc tcgccgctcg gtgacgaagg
    14641 caacaatccg caggatagcg tgacgaatgt ttaccagttt ctgaacaact caagttacgt
    14701 tcatggcaat cacctgttca agtttggcgc ggacctgcgc ttttcgcagc agaatgcttt
    14761 tcgagacgtt gagtcgcgcg ggcgtttaca gttctcaccg ttcttccagt tcactggtaa
    14821 cgcgctcagc gatatgctgc tcggatttcc gctcttgacg agtgtcgcgc gtgtcgataa
    14881 cccgcaaagg attcgcacgg agagttacaa cttcttcatt aacgacagct atcgcgtcac
    14941 gccgcgactg acgctcaacg ccgggttgcg ttacgagtac aactcgccgc cagttgatgc
    15001 tgatgatcgg gcgactattt atgacctcgt tacgcgcagc ctcgtaccgg tcggcacgaa
    15061 cggcgtgccg cggagtggct tcgaagctga taagaacaac ttcgctccgc gggtagggtt
    15121 cgcctggtcc tttaatgaaa agactgtgtt acgtggcggt tatggcattt actacgatca
    15181 gtcaccgctc gcgcccgctg aagcgcttta tttcaactcg ccgttcttcg ataacaacat
    15241 cttcttctcg ttaccgggct taccgctgac gctcaatgat ccgttcccgt cgttcttccc
    15301 gttcccgctg ccggattccg cgctcgcgat tcaacgcgac ttgcgcacgg gttacatgca
    15361 gcactggaac ttcaacgtcg agcgacagat ttttagcggt agcgtactcg aagttgcgta
    15421 cgtcggatcg aagggcacga agttgttgac tgctcgtgac atcaatcagc cgcagccaag
    15481 cgtgctgccg ccgggactgc cgtttgtgcc gcgacctgat ccgcgtttcg atgatatcga
    15541 cttgctcgag tcgcgtgcga attccaatta caacgcgttg caggtgcgtt atcagcagcg
    15601 gctgacgcgt ggctttacag cgctcgcgtc gtatacgtgg tccaagtcga tcgacgacgc
    15661 ttcaaacttc ttcaccagcg ccggcgatcc aaacttcccg cagaacagtt acaacgtcgc
    15721 cgccgagcgt ggtttgtcta atttcgacgt tcgtcaccgt atgtcgttga gttattcgta
    15781 tgcacttcct tttagaaaac caggcgatgg attggtcgcg aacctcgtga acggatggga
    15841 aacgttcggc atcgtcacgc ttcaatcagg acgaccgttc accgtcgcac tgctgtctga
    15901 gatcgataac agcggcacgg gccgatcgat tctcggcttt ggcgcgaacg accggcctaa
    15961 cgtcgtcggt aaccctgagc tttcaaatgg aactacagac caatggttca acaccgccgc
    16021 gttttcgttt ccggcgccgg gtacgtttgg aaatgcgggc aggaatattc tggagggacc
    16081 cggctttcag aacgtcaaca tgtcgattct gaagaacacg cgcctcaacg agcgggtcaa
    16141 tttgcagttc cgggccgagg ccttcaacct cttcaaccac ccgaacttca acttgcccga
    16201 caacttcctt ggctccccaa ccttcggccg gatcacctca gcccgtgacc cacgccacat
    16261 ccagttcggg ttgaaattac ttttttagcc acaaaaaggc accaaagaca caaaagacac
    16321 aaaagaaagc aatgaaggaa ggttcttctt ttgtgctttt tgtgcctttt tgtggctaag
    16381 aggtttgcgg acggtcgtcg tagtagcggt attgggcgct cagcaggagg tcggattctt
    16441 ctgctgaagg ggtttgctgg accatcgtgc cgtcgacttg ttcgtagagc ggtggaggga
    16501 tttcggcgaa ctccgacatt ggaccgtaga ttgaatccac gccttcccta aagacatctc
    16561 caatcaagag attaataatg tggacgcgct cttcgggata cttgcgcaga aagcccgaga
    16621 agctgaagcc gtcgttatag aaggcataga ccagcttgcg catcgactcg ataccgcgat
    16681 cgagtttggc ctggtactgg ccgagacgcg cggcggagaa gtcgtcgtga ttgaaagcgt
    16741 cgtgtatcgc gtcggcagca agctccgcca ttttgagcgc gagcaacacg cctgacgaat
    16801 agatgggatc gagaaaaccg taggcgtcgc cgatgatgat gaagccgtta ccggcgttgt
    16861 gtttcgagcg atacgagtaa tcgagaatcg agtagatctt gtcgacgcga gtaccaggag
    16921 cgacgcgttt gcggaccggt tcacacttat cgatttcctc ctgaaagatc ttcgcgagat
    16981 cctggccacg accctgcacg agatattcag gatcggcaac gatgccgaca ctcgtgatgt
    17041 cgttctcgag tgggatatac cagaaccagc ccccgctacc cgcctcagtt cgcagtacga
    17101 gcgtggcgcc tccgtttaaa tccggctcgc ggtgcgcgtc tttgaagtac gaatagatca
    17161 ccgctttctt gagcttcggg tcgcgcacgc gccagcgaaa cttgttcgac agcatcgcca
    17221 tctggccagt cgcatcgaca acgaccttcg cgaaatactt ctcgcgtgtg ccgtcgggca
    17281 tctgcacttc gacaccaact gcctgatcgc cttcaaacag tacctgcttc acgaggacgc
    17341 cctgatgaac cgtaacaccc tgctcggccg cgtgatcgat cagcaagtgg tcaaactggg
    17401 cgcgcgtgat ttgccaggtg accgcactct cgtgatggac cgcttcgaag aagtagaacg
    17461 gtcgcgactc tttccccgcc gaattcgcaa actgcacact gtactttcga acaaacggac
    17521 tttccttcat tttttcgagt atgcccagcc gtctgaacgt ccagtaagtg tccgtcatca
    17581 gcgattcgcc gatgtgatga cgcggaaaga tctctttttc gaaaaggatt accttgcgcc
    17641 cctcgcgcgc gagaatgccg gccacagtgc tccctgcggg tcctccaccc attacgatga
    17701 cgtcgaaatt attattggta ttcatgcgta gcgtacggtt ctcatttcag gttcaaaaaa
    17761 ttcaggcaga aaatgatcca cttgcaaaag accgtcacga gtcagagcaa cgtggctgtt
    17821 gtgacgttcg agcatgtgct tttcttccaa ctcttcataa acagcacgaa actcgttcca
    17881 aacatcgaca ccgtatttct tctgaaagta accggtgtcg atcgatccgg tctttaattg
    17941 caaaaccatc tcgcgaatga gtcgctgttt tggagtcagt gaaacggccc gcagaagcgg
    18001 caggcggtcc tcgttaagga tgcgcacata ctcttcgaag ttgtgcgcgt tttggaaatt
    18061 gacgccgcca aaatgcgaaa acgacgagac gccgatgccg atcatatcgc cgccatgcca
    18121 gagcgcatcg cgatagatga agcgacaggt ctttttcttc gtcgcgacaa tgtcggtgcc
    18181 cgcgatttcg tagtcgtgcg caagaaattg ttcgaaggcg tagttgagcc agcgacgttt
    18241 cgtatcccag ccggcaatcg gcgaagcggc gcctttcttc aaaatgtcct gcgagatgac
    18301 ggtgttgtag ggcaactcca tctggtagat cgtgacggag tcaggttgaa gctcgatcgc
    18361 gcggcgcaca gcttcacgcc acttctcttc cgtctcgcct gacatacccg cgatgagatc
    18421 gatattgatc tgcgggaagt cgacttcgcg tgcccactgg taagcacgat aaatttcagg
    18481 agataaatga gcgcgaccgt tggcagcgag gatctcgtcg tcgaagtgtt cgatgccaag
    18541 acttaaacgc gtcacgccga tcgctttgag tgtttcgagt tttgctttct gaatcgtgcc
    18601 cggctcacac tcgaacgtga cttcttcggc gttctgccaa ctgacatgtc gattcagacc
    18661 atcgacgaga tagcgcaact gcttctcact gatgtatgac ggtgtaccgc cgccgaagta
    18721 tgcaaagcga agcttgcgtc cctgaaaccc agggctgcga ctgtagagct cgttctcttt
    18781 gatgagcgcg tcgaggtaga cctcgatctc agacgcattc ttgtctgtgt agactttgaa
    18841 gtagcagaac aggcagcgct tccggcaaaa cggaatgtga atgtagagac ctagcggatc
    18901 gtcgacgcgc ggcggctggt tgagcgcgtc gattgcttcg gaaataaact cgggcttcca
    18961 ctgcgaaaac ggcggatagt tcgagacaaa ataattgccg acttcggtac ctgtgctggc
    19021 tgtatcaatc gtcattaatg gttcgcgaac ctgtgtcgct atccgaattc taagatcctt
    19081 acctggaaac ttgcgggaat tgatctctgg ggttgcagct caatcgataa gacgacccgg
    19141 ctaatcgttt agtttcatat ccataaaaaa ttaacatccg gtgcgcggtt gagcaatcaa
    19201 ggtcccagat gatcggggca gtagcccgac cgccttgcag gcttcgataa tcgtcgccta
    19261 aactccccag ctaaatgccg cgaggcgatc attgtcgaat ccgcagtcaa ttactaccaa
    19321 gcattctctc gtttacataa ttctgctggt cacttgcggc gcggggatat ggtttgtttt
    19381 gtggaccggg tcggctctcg atcaaaagcg tgacgtaccg gtcgcc
//
LOCUS       DASSFQ010000246         6464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1746010, whole genome shotgun sequence.
ACCESSION   DASSFQ010000246 DASSFQ010000000
VERSION     DASSFQ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746010"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFH96_08960"
     CDS             <1..1180
                     /locus_tag="VFH96_08960"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HET6974139.1"
                     /translation="YQVTPNTGWRNVSLPARNSNGSPVSNPPLALDLHYLLTAYGAEE
                     LHAEILLGYGMQLLHETPVLTRDAIRRSLTPPSPVEAGGDLPPALAALFTSELAEQVE
                     QIKIVPSALNTEEISRMWSAFQAKYRPTAVYQASVVLIQSRGSTRSSLPVRARRVKVV
                     PFKQPIIQTINSRATPADPIVSGQAILSGFQLALIGFDLSGEETQVLIGGKLVPANQT
                     EVTDSQIIASIPATTPAGVSGVQVIHRTLLGSPPVPHRGVESNVAAFVLQPRIQNIAI
                     ANPQSSGSLRSADINLTVNPAIGDTQRVVMFLNEFIPVTSPPSSGDEVPNSYSFVAPT
                     RRPLSPPTSPPGPSVNITIPIKGVKAGSYLVRIQVDGAESPLGVNAAGQYVSPLLTLV
                     "
     gene            1177..3129
                     /locus_tag="VFH96_08965"
     CDS             1177..3129
                     /locus_tag="VFH96_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974140.1"
                     /translation="MIEATAVNWAEANQQYLMARLLLVREALSQFAKRNEKRTADGDA
                     LLEDLNLATSNLPGPAALDHLCAAFNLSPFERDVLLLCAGVELDSSFAELCIAAQSDS
                     HRAYPTFGLALAALPEAHWSAISPAAPLRYWRLIEVGTNDTITRSQLRIDERVLHYLA
                     GVSGLDARLQGLIEPVHSHATLPSSHQELSQQIARLWQTKDATSWPIINLCGDQPAAN
                     QAVAAFTCHLCNVPLNLLRAVEIPSAVAEREALSRLWEREAALGGGALLIDCDEIENT
                     RALNSLLENVRGMLIVVSREPVRLWRRRIIRFDVRKSSASEQKLLWQQSLGANLDGEL
                     NLLVSQFSLEAQTISTVSALAIANQQLDDASLLNDLWGACRTQARSRLDDLAQRIEPF
                     ATWDDIVLPESQRRTLRDIAAHVRQRSKVYETWGFAARGDRGLGISAMFAGVSGTGKT
                     MAAEVLANELQLDLYRIDLSQVVSKYIGETEKNLRRVFDAAEEGGAILLFDEADALFG
                     KRTEVRDSHDRYANIEVSYLLQRMESYRGLAILTTNMKSVLDTAFLRRIRFIVHFPFP
                     DMVHRAEIWRRIFPFETPTEDLDNSKLARLNIAGGNIRNIALHAAFLAADAGKAVGMR
                     HLLHAARSEYAKLEKPLTDTETANWL"
     gene            3259..3558
                     /locus_tag="VFH96_08970"
     CDS             3259..3558
                     /locus_tag="VFH96_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974141.1"
                     /translation="MKDLPTQVNPAAVELHIEELVLHGFAAGDRYAIAEAVQRELSRL
                     LMVQVAGQGLPPSFAENSEQARLDAGTFKVSPSANCEAIGGQIAQTVHQGLNFRE"
     gene            3551..>6464
                     /locus_tag="VFH96_08975"
     CDS             3551..>6464
                     /locus_tag="VFH96_08975"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HET6974142.1"
                     /translation="MSEHAAAAAPPEKVTAPAIATKQDERLLRRKCACGGGSAGLTGA
                     CKDCDENRLRVQRRALTSTRTFGPTNLVTDVLAEEGQDLDESTRIFMETELGHDFRRV
                     RVHTNARASDSARAVNALAYTVGSDIVFASGSYQPHAHAGRHLIAHELVHTLQQNSQP
                     RGQQAKLEIGEENDGFEREADQIADNVMRRSFTGAVPQPPSPPQLPTSSVAPLIQRTP
                     ATEASAKPEGSESATPVPSLIVEDDAAELGPGQMRKSQFLDELRAAACTAADTELKRV
                     GRSTEACPYIARAFERYRTFEPHRLERGLRRYAPEAAGATSASQYIAAVGARVGRAVG
                     VWATTGEITDVPEELKGEFGVAGVLGGVLSGIGSVVGGLFGGIGKALGGIGKLFFKAR
                     AGGARDEDVDPEEIKSQLGSGQALDAPLRSRMEGAFGHDFSRVRVHANTQAAVLTSSL
                     NARAFTVGTDVGFAAGEYKPGTLIGDALIAHELAHVVQQGDSAQNTAAPMKKETGANS
                     DVLEQDADESAVGAVASIWGGVKHGLTKVSRKAMPRLKSGLQLQRCSHDYEVNGLSTE
                     NAPDSIFFDRRSASIDDSQQKKIENLKSPADRKLRLYAFVSEDENIPPASGAETAHER
                     YVRVDEALRKGPNRHKGDQIAGDSTSPGEDTKSSRGNLNYRNMRKVKVVPASVTSSGE
                     KDCSAGGEQPCSDESKFTSAQTKAPTLLDPAINAISSPLSDSAKKLLDTYFHTTDDAT
                     RDMAASVIRDNLNNVKTHITTQMTPVGTEGTSTVAGKPGHVCANECDPSCGGSTVAYN
                     VDRDADALMTLCDSGTSSAFMSQADETDRALTLIHEGLHGITIVRAPTGGPAPAPSGA
                     KDLAYEWQRVIKYLDTPTALRNNDSYVLFISAVNGLSTKSGADIIDDPEVGEQASRAV
                     AFLQGWLIWAHQEIRALYGVINESRTAGSWSNSYYADTMTRIAGLFEMTVPPATPTE"
BASE COUNT         1456 a         1856 c         1878 g         1274 t
ORIGIN      
        1 gtaccaggtg acaccaaata ccggctggcg aaacgtatcg ctcccggcgc gaaacagcaa
       61 cggctcgccc gtcagcaatc caccgctggc cctcgacctg cattacctct taaccgcgta
      121 cggagcggaa gaacttcatg cagagatcct tttgggctac ggcatgcagt tgctgcacga
      181 aacgccggtg ttgacgcgcg atgcaattcg ccgttcgttg acaccgccat caccggtcga
      241 agcgggtggc gacctgccgc ccgcgctggc cgcgcttttc acctccgaac tagccgaaca
      301 agttgaacag atcaaaatcg tgccctcggc tctcaacact gaagagatct cgcgcatgtg
      361 gtctgcattc caggctaagt atcgaccgac tgccgtgtac caggcatcgg tggtgctgat
      421 tcaaagcaga ggctcgacgc gctcgtctct gccggtgcgc gcgcgccggg tcaaagtcgt
      481 tccgttcaaa caaccgatca tccagaccat caattcgcgc gcgaccccgg ccgatcccat
      541 cgtgtccggt caagcaatac tgtccggctt tcaactggct ttgattggtt tcgatctgag
      601 cggcgaggaa acgcaagtgc tgatcggcgg aaaactggtt cccgcaaatc agacagaggt
      661 gacggacagc cagatcatcg cttcgattcc ggcgacgaca cctgcgggtg tcagcggcgt
      721 tcaggtgatt caccgaacct tgctcggatc tccgccggtc ccacatcgcg gagttgaatc
      781 aaacgtcgct gcattcgtgt tgcaaccgcg cattcaaaac atcgcgatcg caaacccgca
      841 gagttccggt tcactgcgtt cggctgacat caatctcacc gtcaatccgg cgattggcga
      901 cacgcagcgt gtggtgatgt tcctgaacga gttcattccg gttacgagtc cgccatcgag
      961 tggtgacgag gtcccgaatt cctacagctt tgtcgcgccg acgcgcagac ctctgagtcc
     1021 gccgacgagt ccgcctggtc caagtgtgaa cattacgatt ccgatcaaag gagttaaggc
     1081 tggaagttac ctggtgcgaa ttcaggttga cggcgcggag agtccgctgg gagtgaacgc
     1141 agccggacaa tacgtttcgc cattactgac tcttgtatga ttgaagcaac tgctgtgaat
     1201 tgggccgaag caaaccagca atacctgatg gccagactat tgctggtgcg cgaagcgctg
     1261 tctcaattcg cgaagcggaa tgagaaacga accgcggacg gtgacgcgct gctcgaagac
     1321 ttgaatctcg ccacgagtaa cctgcctggt ccagcagcgc tcgatcatct gtgtgcggca
     1381 tttaatctct cgccgttcga gcgcgacgtg ttgctgcttt gtgccggagt tgaacttgat
     1441 tcttcgttcg cggaactctg catcgccgca cagtctgact cgcatcgagc ttatccaact
     1501 ttcggactcg cgctagccgc gcttccggaa gcgcactgga gtgcgatttc acctgcggca
     1561 ccgttgcggt actggcgact aatcgaggtg gggacgaacg atacaattac gcgcagtcaa
     1621 ctccgtatcg acgaacgtgt gttgcattac ctcgcgggtg tgtccggcct cgacgctcgt
     1681 cttcagggac tcatcgaacc ggtccactca cacgccacac ttccgagctc acaccaggag
     1741 ctgagccaac agattgcgcg tctctggcaa accaaagatg cgacaagttg gcccatcatc
     1801 aatctctgcg gcgatcagcc ggctgctaac caggccgtcg ccgctttcac gtgtcacctc
     1861 tgtaatgtgc cgctaaatct gctgcgtgcg gttgagatac cgtcggcggt tgccgaacgc
     1921 gaggcactct ctcgtctatg ggagcgagaa gcggcactgg gcggcggcgc tttgctgatc
     1981 gactgcgatg agattgagaa cacgcgagcg ttgaactcgc tgcttgagaa tgtcagagga
     2041 atgttgattg tcgtgagtcg cgagccagtt cgtttgtggc gtcgacgaat cattcgcttc
     2101 gacgtgcgca agtcgagtgc aagcgaacag aaactactgt ggcagcaatc actgggagcg
     2161 aatcttgacg gcgaactcaa cctgctggtg tcgcagttca gtctcgaagc acagacgatc
     2221 agcacggtca gcgctctcgc aatcgccaat cagcaactcg acgacgcctc tttactgaac
     2281 gacttgtggg gagcgtgtcg cacacaagct cggtcgcggc tcgacgatct ggcccaacgg
     2341 atcgaaccct tcgctacctg ggatgacatc gttctgcccg agtcacagcg tcgcacactg
     2401 cgcgacattg cggcccatgt gcgacagcga tcgaaggtct atgaaacctg gggattcgcg
     2461 gcgagaggtg atcgcgggtt gggaatcagc gcgatgttcg cgggcgtgag cgggactggc
     2521 aagacgatgg ctgccgaagt tttagccaac gagctgcaat tggatctcta ccgcatcgat
     2581 ctcagtcagg tcgtcagcaa gtacatcggc gaaacagaaa agaatctgcg acgcgttttt
     2641 gacgcagccg aggaaggcgg cgcgattctc ctgttcgacg aagcggacgc gctcttcggt
     2701 aaacgcactg aagtcagaga cagccacgat cgctacgcga atatcgaagt cagctacctg
     2761 ttgcagcgga tggagagtta tcgcggtctc gcgatcctta ccacgaacat gaagagcgtg
     2821 ttggatacag cgtttctccg gcgcattcgt tttattgtcc atttcccgtt tccggacatg
     2881 gtccatcgcg ccgagatctg gcgacgcatc tttccgtttg agacgccgac cgaggatctc
     2941 gacaacagca aactagcgcg tctcaacatt gccggcggaa acatcaggaa catcgcgttg
     3001 cacgctgcgt ttctcgcggc ggacgcggga aaagcagtcg ggatgcgcca cttgctacac
     3061 gccgcgcgca gtgagtatgc gaagttggag aagccactga cggacacgga aacggcgaat
     3121 tggttgtagc cgaacaagag ttggtcagtg aacgcctcct tggagtgcgc caacttgttg
     3181 gcgctttggt attggttttc gacgagacgt accaaggcgc caacaagttg gcgcactcca
     3241 aggagtcaca aaaaagaaat gaaagatttg ccaacacaag ttaatccggc agcggtggag
     3301 ttgcacatcg aggagctcgt gctgcacggg tttgctgcgg gtgatagata cgcgatcgcc
     3361 gaagcagttc aacgcgagtt gtcacgactg ctgatggtcc aagtcgctgg ccaggggttg
     3421 ccgccttcat ttgcagagaa ctctgaacag gcgcggctcg acgcaggaac attcaaggtg
     3481 tcgccgagcg caaactgcga agcaattggc ggtcagatcg cgcagaccgt ccatcaagga
     3541 ttgaacttcc gtgagtgaac atgcagcggc cgctgcgccg cccgaaaaag ttaccgctcc
     3601 cgcaattgcg accaagcagg acgaacgact gctgcgtcgt aagtgtgcgt gtggcggcgg
     3661 ctcggcgggc ttaaccggcg catgcaaaga ctgcgatgaa aatcgtctgc gagtgcagcg
     3721 gcgcgcactc acctcaacga ggacttttgg tcccacgaac ctagtcactg acgtgctcgc
     3781 tgaagaagga caggacctcg acgaatcgac gcgcatcttc atggaaacag agctgggcca
     3841 cgatttcaga cgagttcgcg tgcacacaaa tgctcgtgct tccgatagcg cgcgcgccgt
     3901 caacgctttg gcctataccg tcgggtcgga cattgtcttc gcgagcggaa gttatcaacc
     3961 gcacgcgcat gcgggccggc atctgatcgc tcacgagctg gtccacacgc tgcagcagaa
     4021 cagtcaaccg cgtggccaac aggccaaact cgaaatcggc gaagagaatg acgggtttga
     4081 acgtgaagcc gatcaaatcg ccgacaacgt catgaggcga agttttaccg gtgctgtgcc
     4141 ccaaccaccg tcgccaccac agcttccaac gtcatctgtc gcaccgttaa tccagcgtac
     4201 ccccgcgact gaagcttctg ctaaacctga aggttccgaa tccgctactc cggttccaag
     4261 tttgattgtg gaagacgacg cggcagaact cggtcccggt cagatgcgca aatcgcagtt
     4321 tcttgacgag ttgcgcgccg ctgcgtgcac tgcagcagac accgagctca agcgcgttgg
     4381 tcgcagcacc gaagcctgtc cttacatcgc gcgcgcgttt gagcgctatc gaacttttga
     4441 gccgcatcgc ctcgaacgcg gcctgcgtcg ctacgcaccg gaagcagctg gagcaacctc
     4501 ggccagtcaa tacatcgccg ccgtcggcgc gcgtgttggt cgagcggtcg gagtgtgggc
     4561 cacgacgggt gagatcactg acgtacccga ggagctgaaa ggcgagtttg gagtggcggg
     4621 cgtcctcggt ggcgttctgt caggcatcgg cagtgttgtc ggcgggttgt tcggtggtat
     4681 tggcaaagcg ctcggaggta tcgggaagtt gttcttcaag gcccgcgcag gtggggcgcg
     4741 cgatgaggac gtcgatccgg aagagatcaa gtcgcagctc ggttccggac aagcgctcga
     4801 cgcgccactg cggagtcgca tggaaggcgc ctttggtcac gacttctcgc gcgtgcgcgt
     4861 gcatgcaaac acacaggcag cggtgttgac cagcagtctg aacgcacgag ctttcaccgt
     4921 cggcactgat gtcggattcg cggccggcga gtacaaaccc ggcacgttaa tcggcgatgc
     4981 gctgatcgct cacgagttgg cccacgtggt ccaacaaggc gactccgctc agaacaccgc
     5041 cgcgccaatg aaaaaggaga ccggcgcaaa ctcggatgtg ctggaacagg atgcggacga
     5101 gtcggcagtt ggcgcagtgg cttcgatctg gggtggagtc aaacacgggc tgaccaaagt
     5161 atcgcgaaag gcaatgcctc gtttgaagtc aggactccaa ttacagcgct gctcacatga
     5221 ttacgaagtc aacggactat caactgagaa cgcgcctgac tcgatcttct tcgaccgccg
     5281 cagtgcaagc attgacgact cacaacaaaa gaaaatcgaa aatctgaaga gtcccgccga
     5341 ccgtaagtta cgactgtacg ccttcgtcag tgaagacgaa aacataccac ccgcatcagg
     5401 cgccgaaact gcacacgagc ggtacgtgag agttgacgaa gcgttgcgta aaggtcccaa
     5461 ccggcacaag ggcgatcaga ttgccggtga ttcgaccagc cccggtgaag acaccaagtc
     5521 aagtcgcggc aatctcaact atcgcaacat gcgaaaggtg aaggtggtac ctgcgtcagt
     5581 cacctcctcg ggggaaaaag attgctctgc cggcggcgag caaccgtgtt cggatgaatc
     5641 caaattcacg agcgcgcaaa caaaggctcc aaccttattg gatccagcca ttaatgcgat
     5701 tagctctcca ctttctgact cggcgaagaa gctgctcgat acctattttc acactaccga
     5761 cgacgccacc cgcgacatgg cggcttcggt gatccgtgac aatctcaaca acgtcaaaac
     5821 gcacatcacg actcagatga caccagtagg cacggaaggc acaagcaccg tcgcaggtaa
     5881 acctggtcat gtttgcgcta acgagtgtga tccgagctgc ggtggatcaa ccgtcgccta
     5941 taacgtcgac cgagacgcgg atgcattgat gacgctgtgt gatagtggca cttcatctgc
     6001 tttcatgagt caggcggacg agaccgatcg cgctctcaca ctgatccatg aaggacttca
     6061 cggcatcacg atcgttcgcg ctccaacagg tgggccggct ccggcgccga gcggagcgaa
     6121 ggaccttgca tacgaatggc aacgagtgat caagtatctc gacactccga ctgcgcttcg
     6181 aaacaacgac agctacgtcc tctttatcag tgccgttaat ggtctcagca ccaagtccgg
     6241 cgccgacatc atcgacgacc cggaagttgg cgaacaagca agtcgtgccg tcgcttttct
     6301 gcagggctgg ctgatttggg cccatcagga aatcagggcc ctgtatggag tcataaatga
     6361 gtcgagaacg gcagggtcat ggtccaacag ctactacgcc gacacgatga cgcggatagc
     6421 aggtctcttt gagatgaccg tgcctcccgc gacaccgact gaag
//
LOCUS       DASSFQ010000247         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_716770, whole genome shotgun sequence.
ACCESSION   DASSFQ010000247 DASSFQ010000000
VERSION     DASSFQ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716770"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..793)
                     /locus_tag="VFH96_08980"
     CDS             complement(125..793)
                     /locus_tag="VFH96_08980"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HET6974143.1"
                     /translation="MQRNQEPVTVERVVATHRARRKEIRKRLREFQDVWRNGSDARLW
                     EELAYCIFTAGASARMGLNSVDAVRPLLLKGDSDAMTAALKKAGAHRFPVARPRYIVS
                     TRDYFHADCGMALRKRLRSFSDPLERRDWLAQDKQVKGLGYKEASHFLRNIGVKGHAI
                     LDKHVMRCLTEIGVIDSARPPATRRKYLEVEQQLIGFARNVGIDFDELDLVLWSMKTG
                     EVLK"
     gene            1040..2332
                     /locus_tag="VFH96_08985"
     CDS             1040..2332
                     /locus_tag="VFH96_08985"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET6974144.1"
                     /translation="MSWLIGVVLFVATGTFAQVPVESPSPSPSPAPTETARPLYGLQG
                     VLIETLDGKIVASQSENEQFNPASTMKLATALVALRTLGPDHRFATGVWTDGVLDKAT
                     GVINGNLYISGRDPSFHYEHAVLLARELNKLGIKQVSGDLFVAPGFTMNFSASATRSG
                     ERLYDTLDATIRSAEAMRAWNYERTALNDRASLETVPSVAVMGAVAVAPVPPSAKLLL
                     TQKSSKLVDILKVLLCYSNNFMAERIGEALGGPESVRQQLTTQLGLAPDDLRIASLSG
                     LGVNRISPRVMMKIYRELRVELNKHGLLPSAIMPVAGIDPGTLEDRFTGLTARGSVIA
                     KTGTLVRTDGGASSLVGQMQAANGEILLFVIMNQRGSVSRFRENQDYLVMLTQNTRGG
                     PKPFTYRPLMLTMQLSHTESTVAGGSEEYEPPSRSNDQ"
BASE COUNT          539 a          711 c          664 g          540 t
ORIGIN      
        1 ggggagcgca aaacacagca cgaaggcggc cacactgaag gccgccatcc ggaagagaca
       61 cctgttgtta ctacatcgca tgatccacac cttctgagtg atcgtcagct tcgcgcatca
      121 actgttactt caaaacttct ccggttttca tggaccagag aaccagatcg agctcgtcga
      181 agtctatacc gacgttgcga gcgaaaccaa tcagttgctg ctcgacttcg aggtacttcc
      241 ttcgcgttgc aggtggcctg gctgagtcga tcactccgat ttcagtcaaa catcgcatca
      301 cgtgtttatc aagaatagcg tgacctttga cgccgatgtt tcgaagaaag tggctggcct
      361 ctttgtatcc cagtcctttg acctgcttgt cctgcgccag ccagtcacga cgttccaacg
      421 gatctgagaa cgagcgcaaa cgctttcgca acgccatgcc gcagtcggcg tgaaagtagt
      481 ctctggtact gacaatgtaa cgcggtcgcg ccacgggaaa acgatgcgcg cctgctttct
      541 tcaacgcggc ggtcatcgcg tccgagtcgc ctttgagcaa cagtggtcgc acggcgtcga
      601 ctgaattcag tcccatacgc gccgaagcac cggcagtgaa aatgcagtag gcgagttctt
      661 cccagagcct ggcgtccgat ccgttgcgcc aaacatcctg aaactcacgg agcctcttcc
      721 gaatctcctt gcgtcgcgct ctgtgagtgg cgactacgcg ttcaactgtg acgggttctt
      781 gattgcgttg cactgtagtt tgtcaatacg gcgattctat gagcgtgctg gaaacattgt
      841 caacgatatc gctagttgtc cgataaactt cagtttgtcg cgcaatccga caagctaagc
      901 atgtcggaca aatttctgtt atgatgactg cggttggccc cgccgagtgg ttacatccct
      961 catacagtta ctatgcatca aagcttgctc tcctcgcgat actaactata tgacgttgag
     1021 atctgtattt aatttaagag tgagttggct gatcggcgtt gtccttttcg tagctacagg
     1081 cacgttcgct caggttccgg ttgagagccc gtcaccgtca ccatctccag cgccaacaga
     1141 aacagcgcgg ccgctctacg gactgcaggg cgtgctgatc gaaactctcg acggcaagat
     1201 cgtcgcttcg caatctgaga acgaacagtt caatcccgcg tcgacaatga aactcgccac
     1261 cgccctcgtg gcgctacgca cactcggtcc ggaccatcgc ttcgccaccg gtgtttggac
     1321 tgacggcgtg ctcgacaaag caacgggcgt catcaacggc aatctctaca tctccggtcg
     1381 cgatccctcg tttcactacg aacacgcggt gctgctggcg cgtgagctca ataaactcgg
     1441 catcaagcag gtcagcggag atctgttcgt cgcaccgggc tttacgatga atttcagcgc
     1501 ttccgcgaca cgctccggag aacgtctcta cgacacgctc gatgcaacga tccgcagtgc
     1561 cgaagcgatg cgcgcgtgga actacgagcg aaccgcgctc aacgatcgcg ccagtttgga
     1621 aaccgtgccg agcgtcgccg tgatgggcgc agtggctgtt gccccggttc ccccgtccgc
     1681 aaagctgctg ttgacgcaga agtcgagcaa gctcgtcgat attctgaaag tgctgctgtg
     1741 ttattcgaac aacttcatgg ccgagcgcat cggtgaagca ctcggcggcc ctgaatccgt
     1801 caggcaacaa ctcacgacgc agctcggtct tgcgcctgac gatctcagga ttgcttcgct
     1861 gagtggactc ggcgtcaacc gcatctcgcc gcgcgtgatg atgaagatct atcgtgagct
     1921 gcgggtggag ttgaataaac acgggttgct gccgtcggcg atcatgcccg tggctggtat
     1981 cgatccgggc acactcgagg atcgctttac tggactgaca gcgcgcggaa gcgtcatcgc
     2041 caagacgggc acattggttc gaaccgacgg cggcgcgagc tctttagtcg ggcagatgca
     2101 ggctgctaat ggcgagatac tcttgtttgt gatcatgaat cagcgaggca gcgtctcgcg
     2161 cttccgcgag aatcaggatt atctcgtgat gctcacgcag aacacgcgtg gtgggcccaa
     2221 gcccttcacc tacagaccgc tgatgctcac aatgcaactc tctcacactg aaagcacggt
     2281 tgcaggcgga agtgaagaat acgagccacc ctcacgatcc aacgatcaat aaataaaaag
     2341 aagtgcgccc cgtcatccga agatgccggg gcgctttacc gtccaatcac agtcacggcg
     2401 tgcggcaccg caaccacggt ggacgattct cgttgtccgg ttttagttaa agga
//
LOCUS       DASSFQ010000248         9577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1006865, whole genome shotgun sequence.
ACCESSION   DASSFQ010000248 DASSFQ010000000
VERSION     DASSFQ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006865"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..634
                     /locus_tag="VFH96_08990"
     CDS             56..634
                     /locus_tag="VFH96_08990"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6974145.1"
                     /translation="MMVAEQLIDGRVIEACQQGDRAAFQLLFETYKDKVFSIAVYSSG
                     GDRAVADDVTQQIFLKLFTAIRQFRGESEFTTWLYRLVVNACLDEHRRRRRLLPWGET
                     VAMRNPSEKKPQEKQYARLEVAEAVRAAIGELKPKFRLPILLKYIEGLSYEEIASVMG
                     CSKGTVASRLNRGHSQLAKRLSHLNNPAAWGE"
     gene            634..1830
                     /locus_tag="VFH96_08995"
     CDS             634..1830
                     /locus_tag="VFH96_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET6974146.1"
                     /translation="MFSKHVTRDISAYCHGELSHEESKQFAEHTIACAKCRAKFEEVK
                     LGVKLAEQLPQLTAPDHLWRELEPLLDREHVERRPARVSAWQMKAAAAVLVLLSAWGV
                     WLIYSNRDEAILTVGGKPAWRVKRLDGTPTVGTEKISNNGELVVGEWLETDGKSRAQI
                     DVSSIGSVDIDENTRVRLLETHPTEHRLELARGKMCARIWAPPRLFFVNTPSAVAADL
                     GCAYTLEVDDQGSSLLRVTSGWVALELEKRESIVPAGAACETRPSLGPGTPYFEDASN
                     GFRQSLKTIDFDPDAAARSTALNAVLDQARPRDTLTLWHLLTRVEGEDRARVYDKMAT
                     LFPPPAGVTREGILALNQQMLDAWRDAMETTWMGIWKGVPKPLAEAYWKTKNGLSRRL
                     KDLAPK"
     gene            complement(1908..2540)
                     /locus_tag="VFH96_09000"
     CDS             complement(1908..2540)
                     /locus_tag="VFH96_09000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6974147.1"
                     /translation="MPLVTNVLLIRHGQSKGNAERRFGGHTATPLSARGRNQAEATAR
                     TLKSESLTAIYSSDLARALETAKPLANMTGLTVQSTTAFRERDVGVMEGLTFEDAAQQ
                     HPEQYAALLRRDFEYVLTGGESYRQLLDRAVQKLDAIIEEHRGGKIVVFSHTGTICIL
                     ALHLMGALDAPELKPVWISSANCGITKFELRDDGFVRVLAVNDTSHLPKT"
     gene            complement(2545..3621)
                     /gene="prfA"
                     /locus_tag="VFH96_09005"
     CDS             complement(2545..3621)
                     /gene="prfA"
                     /locus_tag="VFH96_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET6974148.1"
                     /translation="MLDKLEQIVSRYEELTQELSSPELLSNPTAYGKAAKQHRTLQEV
                     VNKYQVWKALKAELADARELMDTSDDEEMREMARLEFESLETRIDDADRELKLLLIPT
                     DPNDEKNVILEIRAGTGGDEATLFAAEMVRMYSRYAEKQRWRFEVLEASESGIGGLKE
                     AIVLIEGDKVYSKLKHESGVHRVQRVPQTEASGRIHTSAITVAVLPEAEEVDVKIDAK
                     DLRIDTFCSSGPGGQSVNTTYSAVRITHLPTNTVVSMQDEKSQIKNRDKAMRVLRARL
                     QELEEQKQHEALASERRSMVGSGDRSEKIRTYNFKENRVTDHRIGLTIHQLDLVMEGG
                     LDPFIDALVAHYQAEKLKTDAVAA"
     gene            complement(3632..4807)
                     /locus_tag="VFH96_09010"
     CDS             complement(3632..4807)
                     /locus_tag="VFH96_09010"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET6974149.1"
                     /translation="MSTPERDLIVGGQAVIEGVMMRTPNAYAIAVRKADGTIVNTAAR
                     LPKWSDKYPILKLPVLRGGAVLVQSMGLGIKALNYSANEAFAEAEAAEEVKVALSPAV
                     IEGEGDFAGLTGAVPGLFPVPTQKRSKDELKKGTTGAAVGSIIFALIFNVLLFVAAPL
                     LLTNALFIGAGWATTPAAATSTAAPANTTPAAAEPENEAWYTDAWSSVRTYLHPVRPS
                     VAFNLIDGMIRMTFFLIMIVSFSLLKDIRRVFQYHGAEHKTVFTWEAGLPLTVENARP
                     QPRQHPRCGTSFLMVVMLVAIVLFSVIKFDSLLLNFLIRIVLMPVVAGLSYEIIRLSA
                     KKESSWFFKLMTRPGVWLQNVTTKEPDDNQLEVAIEALKESLKLEPTTGEPQPAPLS"
     gene            complement(4901..5137)
                     /gene="rpmE"
                     /locus_tag="VFH96_09015"
     CDS             complement(4901..5137)
                     /gene="rpmE"
                     /locus_tag="VFH96_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET6974150.1"
                     /translation="MKPGIHPEYVDITVTCACGETFQTRSTKKGDLHVEICSACHPFF
                     TGKQKLVDTAGRVDRFNKRYGKKPAAETAASPQH"
     gene            complement(5310..6284)
                     /locus_tag="VFH96_09020"
     CDS             complement(5310..6284)
                     /locus_tag="VFH96_09020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET6974151.1"
                     /translation="MSNNDAHSNRGRAPLNRTVTLSASELARYQASLEKLEAPASLEN
                     VRDKIFCQDVFTAFPFLPEQSVDLMFADPPYNLTKSFNDRKFKQTSIDEYSEWLDSWL
                     SQTVRILKPTASVYICGDWRSSAAIQRVGEKYFIPQNRITWEREKGRGAKSNWKNCSE
                     DIWFFTVSDEYYFNVDAVMLKRQVIAPYTNSNGEPKDWTETENGRFRVTHPSNLWTDL
                     TVPYWSMPENTDHPTQKPEKLLAKIVLASSREGDLVFDPFNGSGTTTVVAKKLGRHYL
                     GVEIDENYCCFAQKRLEMAEIERTIQGYIDGVFWERNSRRRKAFNRIS"
     gene            6488..7360
                     /locus_tag="VFH96_09025"
     CDS             6488..7360
                     /locus_tag="VFH96_09025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET6974152.1"
                     /translation="MSEDTKQPGSEEPTQPGVSDAEASGTPPPSAIPPAGTLAGADAE
                     KASMVEQAKARVAAVKDSLATEAAPGAPKPPVKKKDEGPKPTDAADHPLVKKLKAQFG
                     DAVGDAVEFIGQLSVNVDAARIVEICDFLRTDSETSFNYLSDLTCVHWPRTPAPFEMV
                     YNLFSIDSNERVRLKARIKDSVDSVTGVWPSANWLEREVFDLFGVRFNNHPDLRRLLL
                     PPDWEGYPLRKDYPLEFVENAWTERHLPEMTDVAKEQLHQRRAYGLEILSVPQERMMR
                     EIFIGGKEVMPKDK"
     gene            7357..7605
                     /locus_tag="VFH96_09030"
     CDS             7357..7605
                     /locus_tag="VFH96_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974153.1"
                     /translation="MTPKEIATQYEAKVFDTPDAAEGAGFVLAETVAPRNVWNKASAA
                     NAIVLKLAEKKEAGEVEEIGLVIEPFRVTGCYKPSERG"
     gene            7626..8759
                     /locus_tag="VFH96_09035"
     CDS             7626..8759
                     /locus_tag="VFH96_09035"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET6974154.1"
                     /translation="MHQVEIATTQETLLDSPEMVLNMGPQHPSTHGVLRVVLKLDGET
                     VIDLDCDIGYLHRGMEKIAENRMYTQIAPYWDRLDYIAAVSNGLVWVETVEQMMGLPV
                     PPRAHYLRTILTELNRIASHLLWLATHALDIGAVTVLLWALREREEILKIFERQFGAR
                     LTCHAWRVGGMPNDAYDDFNKDVRQFVNNFPKRLDEYETVLSENRIWLSRTVGIGVIS
                     AEDAIAIGLSGPALRGSGVPWDVRKSRPYAAYGDMDFDIPVGENGDTYDRYLVRLEEM
                     RQSRRIIDQALENLPDGPVNAKLPKIVKPPVGDYYHSIEAPKGEIGCYLVADGTAQPW
                     RVRVRPPSFINLQALRQMCIGHLVADVVAIIGTLDIVLGEIDR"
     gene            8762..>9577
                     /locus_tag="VFH96_09040"
     CDS             8762..>9577
                     /locus_tag="VFH96_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET6974155.1"
                     /translation="MLLFAPIDVLNDLLLRLGFGEETIRYFVWPLIQIGLVVTLVALW
                     VAYATYLERKISAFMQARLGPMRVGPWGLLQPIADGLKLLVKEDFIPEKADRWIFFFA
                     PYIAVASAFIVFSVIPFGPDWAVIADVNIGLLLVLAVSSVGVLALILAGWSSNSKYAL
                     LGGLRSSAQMVSYEVAMGLSLIGALMFSRTLSLSGIVAAQASDSIWFFVYQPVGFLLF
                     LVSGIAENNRAPFDLPEAESELVAGFHTEYSGMRWSLFFMAEYAAMVVVTGVAT"
BASE COUNT         2057 a         2605 c         2704 g         2211 t
ORIGIN      
        1 aatccgcggc ttatttttgg aagaactctg ctctaacccg cgtctaaagt cctgaatgat
       61 ggtggctgaa cagcttatcg atggacgggt tatcgaagcc tgccagcagg gcgaccgcgc
      121 cgcgtttcaa ctcctgttcg aaacctacaa agacaaggtg ttctcgattg ccgtctactc
      181 atcgggtggc gaccgcgccg tcgccgacga cgtcacgcag caaatatttc tcaagctgtt
      241 tacggcgatt cgtcagtttc gcggcgagtc ggagtttacc acctggcttt atcgtctcgt
      301 cgtcaatgcc tgcctcgacg agcatcgccg ccgccggaga ttgctgccgt ggggagagac
      361 tgtggcgatg aggaatccca gcgagaagaa acctcaggaa aaacagtacg cgcggcttga
      421 agtcgcggaa gcggtgcgcg cggcgattgg cgagcttaag ccgaagtttc gtttgccgat
      481 actgttgaaa tacatcgagg gtttgtcata cgaggagatc gcgagcgtga tgggttgttc
      541 gaaaggcacg gtggcttcac gactgaatcg tggtcacagc cagttagcga agcgactgtc
      601 gcatttgaac aatccggcag cgtggggtga gtaatgtttt ctaaacacgt aacaagagat
      661 atttcagctt actgtcacgg agaactgtcg cacgaagagt cgaagcagtt tgcggagcat
      721 actattgcgt gcgcgaagtg tcgcgctaaa tttgaagaag ttaagcttgg ggtgaaactc
      781 gccgaacaac tgccgcagct taccgcgccc gatcatctct ggcgcgagct cgagccattg
      841 ctcgatcgcg aacacgttga gcgtcgtccg gcgcgagttt cagcgtggca aatgaaagcc
      901 gcggcggctg tgttggtatt gctttcggcc tggggtgttt ggttgatcta tagcaatcgc
      961 gacgaggcga tcttaactgt gggtggaaag cctgcctggc gagtgaagcg actggacggc
     1021 acgccgacag tcggaacaga aaaaatctcg aacaatggag agctggttgt cggtgagtgg
     1081 ctcgagacag acggaaagtc gcgcgcgcag atcgacgtga gctcgatcgg cagcgttgat
     1141 atcgatgaaa acacgcgggt gcgattgttg gaaacacatc ccaccgagca tcgtctcgag
     1201 ctcgcgcgtg ggaagatgtg cgcgcgcatc tgggcgccac cgcgattatt ctttgttaat
     1261 actccgtcag cggtcgcagc cgatctaggt tgtgcttaca cgctcgaagt tgacgatcag
     1321 ggatcgagtt tgttgcgggt gacgtcgggt tgggtggcgt tggagttgga gaagcgtgaa
     1381 tcgatcgtgc cggctggagc ggcgtgtgaa acacgaccgt cgcttggtcc gggcacgcct
     1441 tacttcgaag atgcttcgaa cggttttcgt caatcgctca aaacgatcga cttcgatccg
     1501 gacgcagcgg cacgtagcac agccctgaat gcggtattgg accaggctcg tccccgcgac
     1561 acgctcacac tctggcactt gttgacgcgt gtcgagggcg aagatcgcgc tcgtgtttac
     1621 gacaaaatgg ccacgttatt cccgccacct gcgggcgtca ctcgtgaagg tatactcgcc
     1681 ttgaaccaac agatgctcga cgcctggcgc gacgccatgg agacaacgtg gatgggcatt
     1741 tggaaaggcg tgcctaagcc gctcgcggaa gcttactgga aaacgaagaa cgggctgagt
     1801 cgccggttga aagacttggc ccctaagtga gccacaaaaa agcacaaaga ggaacacaaa
     1861 aggcacaaaa ttaatcgtgt aaattctgaa aatcctgaaa tcctgtctca cgtctttggt
     1921 aggtgactcg tatcgttaac ggctaacacg cgcacaaacc cgtcgtcacg aagctcaaac
     1981 ttcgtaatcc cgcaattcgc actcgagatc cagacgggtt tcaattcagg cgcatccagc
     2041 gctcccatca agtgcaacgc cagaatgcat atcgttccgg tgtgactgaa gaccacgatc
     2101 ttgccgccgc gatgctcctc gatgatcgcg tcgagctttt gtacggcgcg gtcgagtagt
     2161 tgtcgataac tctcgccgcc cgtcaacacg tattcgaagt ctctccgcag caacgccgca
     2221 tattgttcgg gatgttgttg ggcggcgtct tcaaatgtca gaccttccat cacgcccaca
     2281 tcacgttcgc gaaatgcagt cgtgctttga acagtcaaac ccgtcatgtt cgcaagcggc
     2341 ttcgcggtct ccagcgcgcg cgcgaggtcg ctcgagtaga tggcagtgag tgattctgat
     2401 ttgagtgtgc gggcggtggc ttcggcctga ttgcggccgc gtgcggacaa gggagttgcc
     2461 gtgtgaccgc caaagcgacg ttcagcattc ccttttgatt gaccgtgacg aattaaaagg
     2521 acattcgtaa cgaggggcat ttatctaagc cgcgactgca tcagtcttca acttctctgc
     2581 ctggtaatgc gcaacgagcg cgtcgatgaa aggatcgagg ccgccttcca tcacgagatc
     2641 gagctggtgg atcgtcaaac cgatgcggtg atccgtgacg cgattctctt tgaagttgta
     2701 agtgcgaatc ttctcggagc gatcgccgct gccgaccatc gagcgacgct cgctggcgag
     2761 tgcttcgtgt tgcttctgct cttcaagctc ctgcaaacgc gctcgcaaaa cacgcattgc
     2821 tttatcgcga ttcttgattt gcgatttctc gtcctgcatt gaaaccacgg tgtttgttgg
     2881 caagtgcgtg atgcgcaccg cggagtaagt cgtgttaact gactgcccgc ctggaccaga
     2941 cgagcagaac gtatcgatgc gcaagtcctt cgcatcgatc ttcacgtcga cttcttcagc
     3001 ttccggcaat acagccactg tgatcgctga cgtatgaatg cgtccactcg cttcagtctg
     3061 cggcacacgt tgcacgcgat ggacgcccga ttcgtgtttc agcttcgaat agaccttgtc
     3121 gccttcgatc agcacgatcg cctctttgag cccgccaatg cctgactctg acgcttcgag
     3181 cacttcgaaa cgccagcgct gcttctccgc gtaacgcgag tacatgcgca ccatttctgc
     3241 cgcaaacagc gtcgcctcat caccgccagt gccggcgcga atctcgagaa tcacgttctt
     3301 ctcgtcattc ggatcggtcg ggatcagcaa aagtttgagc tcgcggtccg cgtcgtcgat
     3361 gcgcgtctct aacgactcga attcaagacg cgccatctcg cgcatctctt cgtcgtcaga
     3421 cgtgtccatc aactcgcgcg cgtccgccag ctcggctttc aaggctttcc acacctggta
     3481 tttgttcacg acttcctgaa gcgtgcgatg ttgcttcgcg gctttcccat acgcggtagg
     3541 attcgagagc agctcgggag acgaaagctc ctgagtcaac tcttcgtaac ggctaactat
     3601 ctgttccagt ttgtctaaca tgtagacctc attatgacag cggagcgggc tgcggctctc
     3661 ccgttgttgg ctccagcttt aaagattctt tcaacgcttc gatcgcaact tcgagttggt
     3721 tgtcgtcggg ctctttagtg gtgacgtttt gcagccacac gcccggtctg gtcattaatt
     3781 tgaagaacca actactctct tttttcgccg agagccgaat gatctcgtac gagagacccg
     3841 cgacaaccgg catcaacacg atacggatca ggaagttcag caacagcgaa tcaaacttga
     3901 ttactgaaaa gagcacgatc gccacgagca tcacgaccat caggaaactc gtgccgcagc
     3961 gtgggtgttg tcgcggttgc ggtcgcgcgt tctcgactgt caaaggcaga ccggcttccc
     4021 acgtaaacac cgtcttgtgt tccgcgccgt gatactgaaa gacgcggcga atgtccttca
     4081 aaagcgagaa gctgacgatc atgatcaaaa agaacgtcat gcgaatcatg ccgtcgatca
     4141 agttgaacgc gactgaagga cgcactggat gcaggtaagt tcgcacactg gaccatgcat
     4201 ccgtgtacca cgcttcattc tcgggttcag cggccgcagg cgtagtgttc gcaggtgcag
     4261 cggtgcttgt cgcagcagca ggagtcgtgg cccaacccgc gccgatgaag agcgcatttg
     4321 tcagcaacaa cggcgccgcg acgaaaagca gtacgttgaa gatgagcgcg aaaattatcg
     4381 agccgactgc cgcaccggtc gtgcctttct tcaactcatc tttcgaacgc ttctgcgtcg
     4441 gcaccggaaa cagaccgggc accgcgccag tcagaccagc aaagtctcct tcaccctcga
     4501 tcaccgcggg cgagagcgcg actttcactt cttccgccgc ctctgcctca gcgaaagcct
     4561 cgttggccga gtagttcagt gccttgatgc cgagccccat cgactgtacg agcaccgcgc
     4621 cgccgcgcag caccggcaac ttgaggatcg gatacttatc gctccacttg ggcagccgtg
     4681 ccgcggtgtt cacaatcgtg ccgtcggcct tgcggacggc gatcgcgtag gcgtttggcg
     4741 tgcgcatcat cacgccttca ataaccgcct ggccgccaac gatgagatct cgttctggtg
     4801 tactcatgat cttgatctgt agttacaaaa atgccggcgt gtgatgtagt tcgcggttac
     4861 gagctactga cttacgccgg caggctggaa tttgtgatgg ctaatgctgt ggggaagccg
     4921 cggtctcggc ggctggtttc ttgccgtagc gcttgttgaa acgatccacg cgtccggcgg
     4981 tgtcgaccag cttttgttta ccggtgaaga acgggtggca agccgagcaa atttcgacgt
     5041 gcaggtcgcc cttcttggtc gagcgggtct ggaatgtctc gccgcaagcg caggtaacag
     5101 ttatatcgac gtattctggg tgaattcctg gtttcatctt gtcactctct aataggcggc
     5161 acctcgccgc attttcaaac aggatatgat aggagcgcgc acggcgggac gtcaagtttg
     5221 gacaggatta ggtaatgtcc gacaaacttc agtttgtcgc gcaatccgac aagctgaagc
     5281 atgtcggaca tcaaactgag tcctgtaaat catgaaatcc tgttaaaagc tttccttcgt
     5341 ctgctgtttc tttcccagaa cacaccatcg atatatccct gaatggtccg ctcaatttcc
     5401 gccatctcca accgcttctg tgcgaaacaa cagtagttct catcaatctc gacgcctaaa
     5461 taatgccgcc cgagtttctt cgccacgacg gtcgtcgtcc ccgagccatt gaatggatca
     5521 aacaccagat cgccttcacg cgagctcgcc agcacgattt tcgccagcag tttttccggt
     5581 ttttgcgtgg gatgatcggt gttttccggc attgaccagt acggcacggt caggtcagtc
     5641 cacagattcg acgggtgcgt cacgcggaaa cgcccgtttt ctgtctctgt ccagtctttc
     5701 ggctcgccat tgctgttcgt gtaaggcgcg atcacctgac gcttgagcat cacggcgtcc
     5761 acgttgaagt aatactcatc gctaacagtg aaaaaccaga tgtcttccga acaattcttc
     5821 cagttagatt tcgcgccgcg tcctttctcg cgttcccatg tgatccgatt ctgcggaatg
     5881 aaatatttct caccaacacg ctgtatcgcc gccgagctac gccaatcgcc gcatatataa
     5941 acagacgccg ttggcttgag tatcctcaca gtttgcgaca accacgaatc gagccattcg
     6001 gagtattcgt cgatcgatgt ttgcttgaat ttgcggtcgt tgaagctctt cgtgaggttg
     6061 tacggcggat cggcaaacat cagatccact gactgctccg gcaaaaacgg gaatgcggtg
     6121 aaaacatcct ggcagaagat cttgtcccgc acattttcga gactcgcggg cgcttcgagc
     6181 ttctcgagtg aggcttgata gcgtgctaac tccgacgcgg aaagcgttac agttctattg
     6241 agcggagccc gccctcgatt actgtgggcg tcattgttag acacactcac actttaccgc
     6301 agttggaaaa aattcgtgag atcattgtca ccatgaggta atatgaacag cgctttgcta
     6361 taatccgttt tcgctttctt ctcttactcg ctgtaacagc agtgtttacg aagcgattgt
     6421 ctccccgatc ccattgcgga gataattagc tccaccgtag tgtagtgtga acgggctgcc
     6481 ggcataaatg tcagaagata cgaagcaacc aggttcggaa gaacccacac agccgggcgt
     6541 ctcagatgct gaggcgtccg gcacgccacc cccttcagca attccgcccg caggaacgct
     6601 cgccggagcc gacgccgaaa aagcgtcgat ggtcgaacaa gccaaagcgc gcgtggctgc
     6661 agtgaaagat tcactagcga ccgaagctgc gccgggcgca ccgaaaccac ccgttaagaa
     6721 aaaggacgaa gggcccaagc cgactgacgc agccgatcat cctttagtca aaaaactgaa
     6781 agcacaattc ggcgacgcgg ttggcgatgc ggttgagttt atcgggcagc tttccgttaa
     6841 cgtcgacgcc gcacgcatcg tcgagatctg tgattttcta agaaccgata gcgagacaag
     6901 cttcaactac ctctcggatc tgacgtgcgt acactggccg cgcacgcccg ctccattcga
     6961 gatggtctac aacctgtttt cgatcgattc gaatgagcgc gtcagactta aagccagaat
     7021 taaagacagt gttgatagtg ttaccggcgt ttggccctcg gcgaactggc tcgaacgcga
     7081 agtcttcgat ctgtttggtg tgcgtttcaa caaccacccg gatctgcgcc gactgctctt
     7141 gccgccggac tgggaaggct atcctttaag aaaagattac ccgctcgagt ttgttgagaa
     7201 tgcgtggacc gaaaggcacc tgcccgagat gactgacgtc gcgaaggagc agttgcacca
     7261 gcggcgcgcg tatggtctcg agatcctttc agtgccgcag gagcgaatga tgcgcgagat
     7321 atttattggc ggcaaggaag tgatgccgaa agataaatga cgccgaaaga gatcgccacc
     7381 cagtacgaag cgaaagtgtt tgacacgccc gatgcggccg agggggcggg gtttgttttg
     7441 gctgaaaccg tggcgccgcg gaatgtgtgg aacaaggcaa gcgcggcgaa tgcgatcgtg
     7501 ttgaagctcg ctgagaaaaa ggaagcgggc gaagtagaag agattggttt agtgatcgag
     7561 ccgttccgcg tgactggttg ctataagccg agtgaacgcg gataaacgcg gatgaagacg
     7621 gatttatgca tcaggtagag atagcgacaa ctcaggagac actgctcgac agcccggaga
     7681 tggtgctgaa catgggcccg cagcatccgt cgacgcacgg cgtgctgcgc gtggttctca
     7741 agctcgatgg cgaaaccgtg atcgatcttg attgcgatat cggttatctc catcgcggca
     7801 tggagaagat cgccgagaac cgcatgtaca cgcagatcgc gccgtactgg gatcggcttg
     7861 attacatcgc ggctgtctcg aatggtcttg tttgggtgga aacagtcgag cagatgatgg
     7921 gactgcccgt cccgccgcgc gcgcattacc tgcgcacgat tctcactgag ctcaatcgca
     7981 tcgcttcgca cctgctctgg ctcgcgacac acgcgctcga catcggcgct gtcactgttc
     8041 tgctgtgggc gctgcgcgaa cgcgaagaaa ttttgaagat cttcgaacga caatttggcg
     8101 cgcgtttgac gtgtcacgcg tggcgagtcg gtggtatgcc gaacgatgct tacgacgact
     8161 tcaacaaaga cgtccgccag ttcgtcaaca acttcccgaa gcgactcgac gaatacgaga
     8221 ccgtgctctc cgagaaccgc atctggctga gtcgaacagt cggcatcggc gtgatctcag
     8281 ctgaagatgc gatcgcgatt ggtttgtcag gtccggcgct gcgtggctcc ggcgtcccgt
     8341 gggatgtgcg gaagtcgcga ccatacgccg cgtatggcga catggacttc gacattccgg
     8401 ttggcgagaa cggtgatact tacgatcgat acctcgtgcg acttgaagag atgcgacagt
     8461 cgcggcggat catcgatcag gctttggaaa atcttcctga cggtccggtc aacgcgaagc
     8521 tgccgaagat cgtgaagccg cccgttggtg attactacca ctcgatcgaa gcaccgaagg
     8581 gtgagatcgg ttgttatctc gtcgcagacg gcaccgcgca accgtggcgc gtccgggtgc
     8641 ggccaccgtc attcatcaat ctgcaggcac tgcgacagat gtgtattggg catctcgtcg
     8701 ccgacgtggt agcgattatc ggaacgttgg atatcgtttt aggagagatc gatcgctgaa
     8761 catgcttctc ttcgctccca ttgacgtact caacgatctt ctgctgagac tgggtttcgg
     8821 cgaggagacg atcaggtatt tcgtttggcc gctgatccaa atcggtctcg tcgtcacgct
     8881 cgtggcgctc tgggtcgcgt acgcgactta cctcgaacgc aagatctcag cgttcatgca
     8941 ggcgcggctc ggcccgatgc gtgttggtcc atggggactg ctccagccga tcgcagacgg
     9001 actcaagctg ctcgttaaag aagacttcat tcccgaaaaa gccgatcgct ggatcttctt
     9061 cttcgcgccg tatatcgccg tcgcttcggc gttcatcgtc ttttctgtga ttccgtttgg
     9121 tcctgattgg gccgtcattg ccgacgttaa tattggacta cttttggtgc tcgcagtttc
     9181 gagtgtcggc gtgcttgcgt tgattctcgc cggctggtcg tcaaattcga agtacgcgct
     9241 tctcggtgga ttgcgttcga gcgcgcagat ggtttcgtac gaagttgcga tggggctttc
     9301 gttgatcggg gcgctgatgt tttcccggac actgtcttta tcgggaatcg ttgccgcaca
     9361 agcgtccgat tcgatctggt tctttgttta ccaaccggtc ggcttcctgc tctttctggt
     9421 gagcggcatt gctgaaaaca atcgtgcgcc attcgactta ccggaagcgg agtcagaatt
     9481 ggtagccggt tttcacactg aatactcagg gatgcgctgg tcgctgttct tcatggctga
     9541 atacgcggcg atggtggttg taaccggcgt tgcgacg
//
LOCUS       DASSFQ010000249         7701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_266505, whole genome shotgun sequence.
ACCESSION   DASSFQ010000249 DASSFQ010000000
VERSION     DASSFQ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266505"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VFH96_09045"
     CDS             <1..1244
                     /locus_tag="VFH96_09045"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET6974156.1"
                     /translation="GNARAAQDVLHYAATLEPDIATKSIKGTVRIRFTTDSPEPQFNC
                     GELTIESVRLANIPLKFNVHDRKLIVSLPSRTTHAIEIEIDFHGTPRYGIRFLPDRQQ
                     VYTIFSTSQWMVCVDDPADKATVNLKLMLPANLTPIANGQLVSDRILPNKKRLSEWEQ
                     KTPIPTYVIGFTAGPFHVVKEKRGNVEFQYFATNYNESEVRRIFRYTPDMLNFFEERA
                     GVKYSDRTYTQVLAAGNVEQEMSSFTALGESYGKRVLDNEQDQWLGAHEFAHQWWGNM
                     VTCVDWRHFWLNEGIATFIAAAYLEHRFGRAAYMREIEANKTAYEKVRAAGKDKSLVF
                     PDWDHPTRDDRTLVYDKGAYVLHLLREQMGERAFWNGLRLFTRRHFGKSVVTSDFVAA
                     MEEANGKSLKEFFAKWVYLSN"
     gene            complement(1233..2462)
                     /locus_tag="VFH96_09050"
     CDS             complement(1233..2462)
                     /locus_tag="VFH96_09050"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6974157.1"
                     /translation="MKAANTIDDPIRDFYTSHPFPPPVENLDRAREMWADENIQRAEF
                     HLLWPDREYRADLKVLVAGCGTFQAAKHAICHPAAHVTGIDVSVTSLEHTNSLKQKYD
                     LSNLEARQLEIERAADLDQQFDLIICTGVLHHLADPDAGLRALRSVLKPDGAMYLMVY
                     APYGRAGVYMIQDYCRRLGIGTSREEISDLMMVLKYLPQHHPLLATQGGSREFPNGDA
                     LADAVLNPRDRSYSVSQVFDFVESNGLKLSRWYWQAPYLPQCGIIASTPHATKLAALP
                     EREQYIEMELWRGLMPNHSFVAQHSDHDAARVSFADDERFLRYVPIRLPWTTCIVERL
                     PPGAAGLLLNQTHLFQDLFVLINEQEKQIVDAIDGRRSVKEIVELVAGSEPTARKFFE
                     KLWRYDQVVFDTHSSIT"
     gene            complement(2472..3191)
                     /locus_tag="VFH96_09055"
     CDS             complement(2472..3191)
                     /locus_tag="VFH96_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6974158.1"
                     /translation="MNVEQLDERAVARIAGAIGEPARARMLFCLMDGHARTSTELATV
                     ADVSTSTASAHLNRLKAERLVKVLTQGKHRFYSLDGPRVARVLEGLSVLADQPRRKFV
                     PNTPTRLRVARVCYDHMAGHLGVLLHDRFKGLKWLSRAPSVAEDAYELTVEGEKALDA
                     LKINVAELRSSRRRFAYACLDWSERRPHIGGALGSALLDTAIKRKWVTKDLDSRAIRV
                     TNAGRRELLARFGLSTDYTDV"
     gene            3260..3598
                     /locus_tag="VFH96_09060"
     CDS             3260..3598
                     /locus_tag="VFH96_09060"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET6974159.1"
                     /translation="MITCIIRYQIDPFQRDEFKTYAENWGRIIPRCGGHLIGYFLPHE
                     GTNDVAWGLIGFDSLAAYETYRARLRTDSEALANFALAQSKRIILREERNFVQVVEGT
                     FALPPQLEEK"
     gene            3600..4433
                     /locus_tag="VFH96_09065"
     CDS             3600..4433
                     /locus_tag="VFH96_09065"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET6974160.1"
                     /translation="MLTQAEKGSAFTELHRRDGTFIIPNPWDLGSARLLANLGFEALA
                     TSSAGYAFSIGKSDNAIGREAMLAHAASLATATDLPVSIDLENGFGDSPEFVAETIRL
                     AVATGAAGGSIEDATQRSDDPIYERSLAVERIRAAADVVHALPFPFTLTARCENFLVG
                     RLDLKDTIERLQMYQEAGADVLYAPGLTTAEQIAAVVSSVDRPVNVLMALKGVELSVA
                     ELSAIGVKRISVGSALARCALTALLRGAREMKEHGTFRFADDAETFRTINSLFASDDR
                     N"
     gene            4478..4630
                     /locus_tag="VFH96_09070"
     CDS             4478..4630
                     /locus_tag="VFH96_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974161.1"
                     /translation="MIRKLKSGKYRLYSRKVDTKTGKRKNLGTFDTREAAEKHERAVQ
                     YFKRAG"
     gene            4664..5338
                     /locus_tag="VFH96_09075"
     CDS             4664..5338
                     /locus_tag="VFH96_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6974162.1"
                     /translation="MRILLVEDEPRMANVIAKGLREQSYAVDVAQDGAAGLYQAAIND
                     YDLVVLDVLLPQRDGFEVYRELRKRGHTTPVLMLTARASIDDRLTGFDAGADDYLTKP
                     FSFRELLARIRALLRRDSQLRPDVLEIGDLVVDAASHRVSRANHDVQLTAKEYALLEY
                     LARRAGELVSRADIAAHVWDDTFDPFSNAIEVYINRLRKKIDGQHKNKLLHTRRGEGY
                     ILEYRH"
     gene            5410..6750
                     /locus_tag="VFH96_09080"
     CDS             5410..6750
                     /locus_tag="VFH96_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6974163.1"
                     /translation="MENRGRPKVFPLAVLLVLLCVSVISTAQTPSPTPETPALSLDEA
                     LRLAIAQASTFQTALLNERVAAEDLRQARAAFLPKVSAPLSYIYSSPALGLPPGEPRG
                     PSFVTSDGIGVYDGLVNVSGDFDIAGRLRATLAKNRALLAAAHAGADVARRELAQAVI
                     EAYYSLALAGAQRRAAETNLAAAEEFEHITSLLLSGGEVAAVDLTRAQLQTLTRRDEL
                     EKARGNEAVAAGSLRVLVGYDFSRPINTTDLALALPSNSGLGQFKADDVARRPEFTQL
                     EQQLRAAQQEIKIARADRLPSLSYSINGGFDTDSLKGPRLKEHSGVSAAISFSIPIFD
                     WGASRSRERQAQLRVDIAENERTMALRGFTQQFYAARAQVDNAAVRINLAREGVTKAQ
                     DNLTASIARYRAGEAQIVEVTDAQTTLVEQRTALYQAIFDYQTALARLRQATGQ"
     gene            6773..>7701
                     /locus_tag="VFH96_09085"
     CDS             6773..>7701
                     /locus_tag="VFH96_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6974164.1"
                     /translation="MSDQPNRIPDDEVDRLLAEQKRARIVRQRNIVLIVGSGLVVLLL
                     VALVVWRLKRSATEAESEVTPVVSVKVVKAEKESIAAPVSAVGTIWPREKADVAAKIS
                     AQIKKMFLLKNKLVHAGDVIAVLESRDLETQRAEAVAALNEARANERSLITGTIPKTN
                     AEDQKALLDARAKVNNARATYDRRRALFERGGISKKDLEASQLELTTAEDELRLQEQT
                     VALRVASLNPNDRALAAARTAQAQQRVATLDAQLSYATIRSPITGIVTDQFQYEGEFA
                     SAGGKLVTIADTSSVIVKAPFADTAVAELKTGDT"
BASE COUNT         1802 a         2228 c         2101 g         1570 t
ORIGIN      
        1 gcggaaacgc acgcgcggcg caggatgtgc tccattacgc cgcaacactc gagcccgaca
       61 ttgcaacgaa atcaatcaaa ggcacagttc gcatccgttt cacaaccgac tcaccggaac
      121 cacaattcaa ctgcggcgag ctcacgatcg aatccgttcg cctcgcaaac atacccctca
      181 agttcaacgt tcacgaccgt aaactcatcg tgtctctccc atcccgaacc acacacgcca
      241 ttgagatcga gatcgacttt cacggcacgc ccagatacgg aattcgcttt cttcctgatc
      301 ggcaacaggt atatacgatc ttctcaacca gccagtggat ggtctgcgtg gatgatcctg
      361 ccgacaaagc aacagtaaac ctgaaactga tgctgccggc gaacttaacc ccaatcgcca
      421 acggccagct cgtctccgat cgcatcttgc cgaacaaaaa gcgcctctcc gagtgggaac
      481 agaaaacccc aatcccaact tacgtcatcg gtttcacagc cggccctttt cacgtcgtta
      541 aagagaagcg aggaaacgtc gagttccaat acttcgccac gaactacaac gagagcgaag
      601 tgcgtcgcat ctttcgctac acgcctgaca tgctcaactt cttcgaagag cgcgcggggg
      661 tgaagtattc agatcgcacg tacacgcagg tactagcggc cggcaacgtt gaacaggaaa
      721 tgagtagctt caccgcgctg ggagaaagtt acgggaagcg ggttctcgac aatgaacagg
      781 atcagtggct tggcgcgcac gagtttgcgc atcagtggtg gggcaacatg gtgacttgcg
      841 tcgattggcg gcacttctgg ctcaacgaag gcatcgccac gttcatcgcc gccgcatatc
      901 tggagcatcg tttcggacgc gcggcgtaca tgcgtgagat tgaagcgaat aaaacggcct
      961 acgaaaaagt acgcgccgcg ggcaaagaca aatcgctcgt gtttccggat tgggaccacc
     1021 ccacgcgcga cgatcggacg ctcgtctacg ataaaggcgc gtacgtatta catctactgc
     1081 gcgaacagat gggcgaacgc gcgttctgga acggtctgcg actcttcact cgcaggcact
     1141 tcggcaaatc cgtcgtcacc agtgacttcg tcgccgcgat ggaagaagca aacggaaaga
     1201 gtttgaaaga atttttcgcg aagtgggttt acttaagtaa ttgagctgtg cgtatcaaac
     1261 accacctgat cgtaccgcca gagcttttca aagaacttgc gcgccgtcgg ttcagatcct
     1321 gccactaact cgacgatttc tttcacgcta cgacgcccgt cgatcgcatc gactatctgc
     1381 ttctcctgtt cgttgatgag cacaaataga tcttgaaaga ggtgcgtttg gttcaacaac
     1441 agaccggcgg cacccggcgg cagacgttct acgatgcaag tggtccaggg cagtcgaatc
     1501 ggcacgtaac gcagaaagcg ctcgtcatcg gcgaagctta ccctcgccgc gtcgtggtcg
     1561 ctgtgttgag ccacgaaact gtgattcggc atcaagccgc gccagagctc catctcgatg
     1621 tactgctcgc gctccggcag cgccgctagt ttagttgcgt gcggtgtgga agcaatgatt
     1681 ccacactgcg gcaagtaagg cgcctgccag taccagcgcg acagtttcag gccgttgctc
     1741 tccacgaagt caaacacttg cgaaacggag tacgaacgat cgcgcggatt caagaccgcg
     1801 tcagctagag catcgccgtt cggaaactcg cgtgagccac cttgcgtggc gagcaacgga
     1861 tgatgctgcg gcagatactt cagcaccatc atgagatcgc tgatctcctc tcgcgaagtt
     1921 ccgataccaa gccggcgaca gtaatcctga atcatataaa cgccggcacg gccatacggc
     1981 gcatacacca tcaagtacat cgctccgtcc ggcttcaaaa ccgatcgcag cgcgcgcaaa
     2041 cccgcatccg gatcagcaag gtggtgcaac actcccgtgc agataatcag atcaaactgc
     2101 tggtccagat ccgccgcgcg ttcgatttcc aactgtcgcg cttcgagatt tgataagtcg
     2161 tatttttgtt tcagactgtt ggtgtgctcg agactcgtca cactgacgtc gatgcccgtc
     2221 acgtgtgcag cgggatgaca aatcgcgtgc ttcgccgcct gaaacgtgcc gcatccggcc
     2281 acgagcacct tcagatcagc tcgatattcc ctgtcgggcc acagcaaatg aaactcggcg
     2341 cgctgaatat tctcatctgc ccacatttcg cgggcgcgat cgagattctc aaccggtgga
     2401 ggaaacgggt gactggtgta gaagtcacga atcggatcat cgattgtatt cgcggctttc
     2461 atagatattc actatacatc tgtgtaatct gtggacaaac cgaatcgcgc caacagctca
     2521 cgacgtcctg cattcgtaac gcgtatcgcc cggctgtcga gatcctttgt cacccatttt
     2581 cttttgatcg cggtgtccaa caaagccgaa cccaaagcac caccgatgtg tggacgacgc
     2641 tcactccaat caagacacgc gtaagcaaaa cgacggcgcg aacttcgcaa ctccgcgacg
     2701 ttaatcttga gagcatcaag cgccttctct ccttcgaccg tcagctcata cgcatcttcg
     2761 gcgacactcg gcgcgcgaga taaccacttg agacccttga atctgtcgtg caacaaaacg
     2821 ccgagatgac cagccatgtg atcgtaacag acacgggcaa cccgcagacg cgtgggtgtg
     2881 ttgggaacaa acttgcggcg cggctggtcc gcgagcacgc tcaggccctc gaggacgcgt
     2941 gcgactcttg gtccgtcgag gctgtaaaag cggtgcttac cttgtgtcag cactttgacg
     3001 agccgctccg ccttcaaacg attgaggtgc gcgctcgcag ttgaagtcga aacatcggca
     3061 acggtcgcaa gctcagtgct ggttcgcgca tgtccatcca tcaagcagaa aagcattcgc
     3121 gcacgcgccg gctcaccaat cgcgcccgcg attctcgcca ccgcccgctc atcgagttgt
     3181 tctacattca tagtttgatg ataaacgaat cgtctccgca cgacaactga tataaccagt
     3241 aatcaaagga gaagcatcta tgatcacctg catcattcgt tatcaaatcg atccgtttca
     3301 acgcgacgag ttcaagacgt acgcggagaa ttgggggcgc atcattccgc gctgcggcgg
     3361 ccatctcatt ggttacttcc tgccacatga aggcacgaat gacgtcgcct ggggactgat
     3421 cggttttgac agcctggccg cgtacgagac atatcgcgcg cgtttgcgca ccgattctga
     3481 agcgctcgcg aacttcgccc tcgcgcagag taaacgtatc attcttcgcg aagagagaaa
     3541 ttttgtgcag gtggtagaag ggacgtttgc actgccgccg caactcgagg agaaataaca
     3601 tgctgacgca agctgaaaaa ggcagcgctt ttaccgaact gcaccggcgc gacggaactt
     3661 tcatcattcc gaatccgtgg gatctcggga gcgcgcgact gctcgcgaat ctcggcttcg
     3721 aagcgctggc taccagcagc gctggatatg ccttttcgat tggaaagtct gacaacgcca
     3781 tcggtcgtga ggcgatgctc gcgcacgctg cgtctctcgc cacggccacc gatctgccgg
     3841 tcagcatcga tttggagaac gggtttggcg attcacctga gttcgttgcg gaaacgattc
     3901 gtctcgctgt cgcaaccgga gctgccggtg ggtcaattga ggatgcaacc cagcgctccg
     3961 acgatccgat ctacgaacgt tcgcttgcag ttgaacgcat tcgcgcagcc gctgacgtcg
     4021 tgcatgctct tccttttccg ttcaccctga ctgcgcgttg cgagaacttt cttgtcggca
     4081 ggctcgatct gaaagatacg atcgaacgtt tgcagatgta tcaggaagca ggcgccgatg
     4141 tgctttatgc gcccggtctc acaactgcgg aacagatcgc agctgtcgtg agttctgtgg
     4201 atcgacctgt caacgtgttg atggcgctga aaggtgtcga attgagtgtg gcggaattgt
     4261 cggcgatcgg cgtaaagcgc atcagcgtgg gcagcgcact ggctcgctgt gcactaacag
     4321 ctttgctccg tggcgcgcgt gaaatgaaag agcacggaac gtttcgcttt gccgacgacg
     4381 ctgagacttt ccgcacgatc aattccctct tcgcgtctga tgatcgcaac taaactttga
     4441 caggattaac aaaattcttg tgtacgatta agaagcaatg atcagaaaac taaaatccgg
     4501 aaagtatcga ctctactcgc gcaaagtcga tacgaagacc ggcaaacgca agaatctcgg
     4561 cacgtttgat acacgcgaag cagcggagaa acacgagcgg gcggtccagt atttcaaacg
     4621 cgccggttga tcatggctca tcagaggcgc agaatctgcg cagatgcgga ttctcctggt
     4681 tgaagatgag ccacgcatgg cgaatgtgat cgcaaagggt ttgcgagaac agagctatgc
     4741 ggtcgacgta gcgcaggatg gcgcggcagg tctgtatcag gctgcgatca acgattacga
     4801 cctcgttgtt cttgatgtgt tgttgccgca acgcgatgga tttgaagtct atcgtgagtt
     4861 gcgaaagcgt ggccatacaa cgccggtctt gatgctgacc gcccgagcgt ccatcgacga
     4921 tcgtctaaca ggtttcgacg cgggcgccga tgattatctg accaagccgt tttcgtttcg
     4981 cgagttgctg gctcgcattc gcgcgctgtt gcgacgcgat tcacaactgc gtccggacgt
     5041 gctggagatt ggtgatctag tcgttgatgc cgccagccat cgtgtttccc gggccaatca
     5101 cgatgtgcaa ttgacggcga aggagtatgc gctgctcgaa tatctcgcgc gtcgagcggg
     5161 cgagcttgtg tcgcgcgcgg acattgcggc gcacgtctgg gacgacacct tcgacccctt
     5221 ctcaaacgcg attgaagtct acataaaccg tctgcgcaaa aagattgacg gccaacacaa
     5281 aaacaaactt ctccacactc gccgcggcga aggatacatc ctcgagtatc gccactaatt
     5341 cgtgatggtc tattaatctt ctattaagga acgacttcat atactcgact tcgaaacgaa
     5401 ccgaggaaca tggagaatcg tggacgaccg aaggtattcc cccttgcggt actgctggta
     5461 ttgctctgcg tctctgtaat ctcaaccgcg caaactcctt cgcctacacc agaaaccccg
     5521 gcgctttcgc tcgacgaagc gttacgactc gcaatcgcgc aagcatcaac ttttcagaca
     5581 gcactcctga acgaacgcgt cgccgccgag gatctgcgac aggcacgcgc ggcgtttctt
     5641 ccgaaagtca gcgcgccact gagttacatc tacagttcgc cggcgctcgg cctgccgccg
     5701 ggtgagccac gcggtccaag ttttgtgacc agcgatggca tcggcgttta cgacgggctc
     5761 gtcaatgttt cgggcgattt cgatatcgcc ggtcggctgc gtgcaacact cgctaagaat
     5821 cgtgccttgc tcgccgcagc tcacgcaggc gctgacgttg cgagacgtga gctcgcgcag
     5881 gcagtgatcg aagcttatta cagcctcgcc ctcgcaggtg cacaacgtcg tgcggcggaa
     5941 acaaacctgg cggccgcgga agaatttgaa cacatcactt ctttgctgct cagcggcggt
     6001 gaagtcgcag cggtcgatct gacgcgcgct caattgcaga cgctcacgcg tcgcgatgaa
     6061 ctcgaaaaag cgcggggcaa cgaagccgtc gccgccggat cgttgcgcgt gctcgtcggg
     6121 tacgacttct ccagaccaat caacacgacg gatctcgctc tcgcgcttcc ctcaaattca
     6181 ggactcggac aatttaaagc tgacgacgtc gcgcgccgtc cggagttcac gcaactcgag
     6241 caacagctcc gcgccgccca gcaggagatc aagatcgctc gcgccgatcg tttgccgtcg
     6301 ctctcgtatt caatcaacgg cggtttcgac accgattcgc tgaagggacc acggctgaag
     6361 gagcacagcg gcgtttcggc cgccatcagt ttctcgattc cgatctttga ttggggcgcg
     6421 agtcgcagcc gcgagcgcca ggcacaactc cgcgtagaca ttgctgaaaa cgaacgaaca
     6481 atggcattgc gtggattcac gcaacagttt tacgcggcgc gcgcgcaagt tgataacgcg
     6541 gcggtacgaa tcaatcttgc acgcgaaggt gtcacgaaag cacaagacaa tctaacagct
     6601 tcgatcgcgc gttaccgagc cggcgaagcg cagattgtcg aagtgaccga cgcacagaca
     6661 acgctggtcg aacaacgaac cgctttgtac caggcgatct tcgattacca gaccgcgctt
     6721 gcgcgactgc gacaagcaac cggtcagtga gaaacaaaca cgagtggcga atatgagtga
     6781 tcagccgaac agaattcccg acgacgaagt cgatcgcctg ctggccgagc agaaacgcgc
     6841 acgcatcgtg cgccagcgaa acatcgttct gatcgtcggc agcggactcg tagtgctgtt
     6901 gctcgtggca ctcgtcgtct ggcgcttgaa gcgatcggca accgaagcag aaagcgaagt
     6961 aacgccggtt gtcagcgtca aagtggtgaa ggctgagaag gaaagcatcg ccgccccagt
     7021 gtccgcagtg ggcacgatct ggccccgaga aaaggctgac gttgccgcga agatcagcgc
     7081 gcagatcaaa aagatgtttc tgctgaagaa caagctggtc catgccggcg acgttattgc
     7141 cgtactcgag tcgcgcgatc tggagactca gcgtgccgaa gccgtggccg cactcaacga
     7201 ggcgcgcgcg aatgagcgca gtctcattac cggcacgatt ccaaagacaa atgccgaaga
     7261 tcagaaagca ctgctcgacg cacgcgccaa ggtcaataac gcgcgcgcca cttacgaccg
     7321 ccgtcgcgcg ctctttgagc gaggtgggat ctccaaaaaa gatctcgaag cctcacaact
     7381 cgaactgacc accgccgaag atgagctgcg cttgcaggaa cagactgtag ctttgcgtgt
     7441 cgcatcgttg aatccaaacg atcgtgctct cgcagcggct agaacagcac aggcacaaca
     7501 acgagtggcg acgctcgatg cgcagttgag ttacgccacg atccgttcgc cgatcactgg
     7561 aatcgtcacc gaccagtttc agtatgaagg cgaattcgct tcagcaggcg ggaaactcgt
     7621 tacgatcgcc gacacgagct cggttatcgt gaaagctcca tttgccgaca ccgcggtggc
     7681 tgaactcaag acgggcgaca c
//
LOCUS       DASSFQ010000250         7879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_425275, whole genome shotgun sequence.
ACCESSION   DASSFQ010000250 DASSFQ010000000
VERSION     DASSFQ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425275"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFH96_09090"
     CDS             <1..790
                     /locus_tag="VFH96_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6974165.1"
                     /translation="AYKTYATVSGRRFVSDLRDALAKGYISRMPSYNSIFDYSKMESL
                     TPYLKQLIAQSALPLQSIESDFAVDSSGFSTTNYMRWFDVKYGNTEDWHEWIKMHVAC
                     GVRTHVVTGVELTSARTHDAPYLKPLLAQTAQAGFKMQEVSADKGYISGDNLQAVVDH
                     GATPYMPFKTNVTGKRGSELWKKLFHYYSYKREEFLTHYHKRSNVETVFSMIKARFGE
                     RLRSKSETAQINEALTKVLCHNLCVVIQSMYELDVTPEFWSEAA"
     gene            complement(828..1919)
                     /locus_tag="VFH96_09095"
     CDS             complement(828..1919)
                     /locus_tag="VFH96_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974166.1"
                     /translation="MRRSLSNFKPNRVLLIGHSLTSSMPEQNTNSPLFADQEAVPGVL
                     TDVEIEAAVSRGALITGSTFHATSLEPSSYDIRVGRKGIIGGQGVELDLSQSPMELEP
                     GAYGGVVSYERLHLPSNVCARIGSKRALSYDGVILLTGSLVDPGYEGHLLFGLYNASQ
                     RRVLIRSGRKICNIVFERLSDSPERQAPSDPNLQNGAFPDAFVDRMANMDVLPWMQIS
                     ERVKQIETITKDILDLKARYDDVLGPIRDLTNNVKSLTSDVTSLTEQTRSIAKDVENV
                     NTMVGENSRQINTLTTNLATMVAQVHAVQERTRNLEEEEKTQGETITTLRTRLGRFQL
                     LIYIFWAILLLVLGGVITRVFAVYFLKQG"
     gene            2185..2949
                     /gene="larB"
                     /locus_tag="VFH96_09100"
     CDS             2185..2949
                     /gene="larB"
                     /locus_tag="VFH96_09100"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET6974167.1"
                     /translation="MDLNELAKLLAAYKSGELETNEAVDRIKNLHFEDIGFARVDHSR
                     ASRQGFPEVVFGGGKTRAQIVSIVDRLVQRSPNILVTRTDESTFGEIRNVVTDAEWHE
                     AARLVRIRRDKTNHGVGSIAVVTAGTSDIPVAEEAALTAETMGNTVDRIWDAGVAGIH
                     RVLAERSLLQRSRVVIVVAGMEGALPSVVGGLVGVPVVAVPTSIGYGASFGGVAALLG
                     MLNSCASNVTVVNIDNGFGAGFVASLINRRWTVPVE"
     gene            2942..4333
                     /locus_tag="VFH96_09105"
     CDS             2942..4333
                     /locus_tag="VFH96_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974168.1"
                     /translation="MNRPAVAYSRRLIFVLIAVSLVSSGCISARRTPNLEQIFATARA
                     RTGKRPVIVIPGILGTELMNPKTGEIVWPSAFRTSQEGLPISPDLAANRDDLIPGKII
                     ETVKLARVLPEVYVYRDLLVALRRYAGYRDGDWNNPAADGYEDTFYVFPYDWRQDNVS
                     NARELIRRISQLKTKLQRPDLKFNIVAHSMGGLIARYAAMYGDADLPPGNGPIQPTWQ
                     GAADISKIVMIGVPNEGSTDAFATLVEGYSITEGLRRRVPLLNKLTAEDVVSAPAVFQ
                     LMPHARVVRFLDENLQPLAVDLYDPEVWKRYGWSVIYSSPDFRRQHAGDLDAYLTATL
                     QRARRFHEALDAMRPEDTPVILLAIGGDCEETLSSPVIVRDQKRNRWVTLTRPREFRT
                     SSGVKISKQKATDAMYAPGDGRVTRQSLLGENIFKSLSRYAIFGCDLHGQLPRNPSLQ
                     DNALTAIVNEVIN"
     gene            complement(4399..5832)
                     /locus_tag="VFH96_09110"
     CDS             complement(4399..5832)
                     /locus_tag="VFH96_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6974169.1"
                     /translation="MKFVWILLFLLIPHPIEASQDLLPELVRRIKPSAVAIETFDSKG
                     EKLSRGSGFFVEADRIVTNRHVLEGAYRAEVHSSSGAVFPVKGVLAVDAEGDIALLKI
                     DAPAPPVRPLPLDKTSPQEGESVVVIGNPLGLEGSVTNGIVSAVRDIPTFGRIIQITA
                     PISSGSSGSPVVNMQGQVIGIATLQITGGQSVNFAIPSERISQMQVAQTQTMSSLADL
                     VASTGRNKRAKAVQFFRDGLSFLSKDDCEKALPYFEKAVESDSNYAEAWAQTGFCNEK
                     LGRHAEALEASKKAVSLRPSAESYFNIGLASFYLKQYKDAENGYRQSIKLDPYNAADA
                     QYALGLVYRDWGKPDDEIQAYKQAIRLRPDYTVAYERLGSRYLKSKKYSEAVEVFRQL
                     AALKPGDANAPNNMGEAYFELNKLNEAVESFRQSIRLKPDFGKAYYNLGRTLMALGNR
                     DAALEQYTILTNIDPDYAEKLNAIINP"
     gene            complement(5848..7569)
                     /locus_tag="VFH96_09115"
     CDS             complement(5848..7569)
                     /locus_tag="VFH96_09115"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET6974170.1"
                     /translation="MRWSRYFIPTLREDPADAEVISHKLLLRAGLIRQLSAGIYSFLP
                     LGQRVAIKVMQILREEMNAIGGQEFFLPALHPAEIWQESGRWYAIGDEMFRLKDRKGT
                     DMCLGMTHEEVFTTIARSELRSYKQLPQVWYQIQTKFRDEARPKSGLMRIRTFIMKDA
                     YTFDVDRAGLDKSFIDQREAYKTIFTRCGLQFLIVEASSGSMGGSESNEFMAPSPAGE
                     DVIVSCTNCDYAANLEKATSRLAQIADEPGPAAPEEFPTPGVRTIEDLITFPGGADAN
                     RQIKSLVFIATIDGESRPVLVLLRGDHQLHETRLSDSIRATAVRPAHPEEIRDLLGAS
                     AGSLGGVGAKNRSRDLRIVADSALQNRRNMTTGANKDDHHLRGVDITRDIPVDEWFEL
                     RTVEAGEGCPRCETGTLEIFKGMEIGHIFKLGTKYSASMGATVLTQDGKEVPIVMGSY
                     GIGVERIITAAVEQHHDDDGIIWPKSLAPFDVVVTITNMKQDDLRNAGEKLYEELQGA
                     GFDVLLDDRDERAGVKFKDADLIGIPYRITIGKKIADGMVELFDRSAKKSEDVRIGDV
                     VARLRHV"
BASE COUNT         1750 a         2174 c         2130 g         1825 t
ORIGIN      
        1 agcgtacaaa acttatgcga ctgtgagcgg tagacggttt gttagtgatc tccgcgatgc
       61 gttggcaaag ggctatatct cgcggatgcc ttcttacaat tcgattttcg actattccaa
      121 aatggagtca ctcacgcctt acctgaaaca gttgatcgcg cagagtgcgt taccgcttca
      181 atcaatcgaa tcagactttg ccgttgatag ctctggtttt tcaaccacga actacatgcg
      241 atggttcgat gtgaagtacg gcaacactga ggactggcat gagtggatca agatgcacgt
      301 tgcttgcgga gtgcgaacac atgttgtaac gggcgttgaa ttgactagcg cacggacgca
      361 cgatgcgccg tacttgaaac ccttgcttgc acagaccgca caggccggat tcaagatgca
      421 agaagtatca gccgacaaag gttacatcag cggcgacaat ctacaggcgg ttgtagatca
      481 cggcgcaacg ccttacatgc cattcaaaac gaacgtgaca ggcaagcgcg gtagcgagct
      541 ttggaaaaag ctttttcatt actacagcta caagcgcgaa gaattcctaa cgcactatca
      601 caagcgcagc aacgtggaaa cggtctttag catgatcaag gcacgtttcg gagagcgttt
      661 gcggagtaaa tctgagacag cgcagatcaa tgaggcactc actaaggttc tctgtcacaa
      721 cctctgcgtt gttattcagt caatgtatga attggatgtc acgcctgagt tttggagtga
      781 ggcggcgtaa aaggcgtgtc tgcgccctcg ctcatactga cagaacccta gccttgcttg
      841 aggaaataaa cagcaaaaac tctagttatc actcccccca agactaaaag caagatggcc
      901 caaaaaatat aaatgagcaa ctgaaaacgt cccagtcttg tgcgaagagt cgttatagtt
      961 tcgccttggg ttttttcttc ctcctccaaa ttgcgggttc gttcttgaac tgcgtgtacc
     1021 tgcgcaacca tcgtagcgag atttgtggtc aacgtgttta tctgtcgact gttttctcca
     1081 accatggtgt taacattttc aacgtccttc gcaattgatc tcgtttgctc cgttagactg
     1141 gttacgtcgc tcgttagaga ctttacattg tttgttagat cgcgaatcgg cccaagcacg
     1201 tcatcataac gggcttttag atccagaata tcttttgtaa tggtttcaat ctgcttgacc
     1261 cgttcgctta tttgcatcca cggaagcacg tccatgtttg ccattctgtc aacgaaagcg
     1321 tcagggaacg ctccgttttg cagattagga tctgaaggcg cttggcgttc tggagagtcc
     1381 gatagtcgct cgaaaacaat attgcatatc tttcgaccac ttctaatcag gacgcggcgt
     1441 tgggatgcgt tgtatagacc aaagagtaga tggccctcat agcccggatc aacgagagaa
     1501 cccgttagaa ggataacgcc atcgtaagaa agcgcccgct ttgaaccaat gcgagcacag
     1561 acattagaag gtaagtgcag acgttcataa gaaaccacgc caccataggc ccccggctca
     1621 agttccattg gtgattggga caaatcaagc tccacacctt gacccccaat gatccctttc
     1681 cggcccactc gtatgtcata gcttgaaggc tcaagggatg ttgcgtgaaa agtgctgccc
     1741 gttatgagtg cgccacgcga gaccgcggct tcaatttcga catccgtgag aacgccgggc
     1801 acagcttctt ggtcagcgaa tagaggggaa ttagtgtttt gttcgggcat tgaggacgtt
     1861 aagctatggc cgatcaataa tacccggttc ggcttgaaat ttgaaaggga acggcgcata
     1921 taatccttta cccggtaaga tgtcgcatct tacacgatgt ttcacgcgcg aaacaagctg
     1981 atttctaata gtggcgcttc gtgcaaacgc tacggtttaa gcacttatcg ggcaagtccc
     2041 acaaccagga ttagcttctc gggcaagatg cactattcgg gcaaagccgc ttgaactctg
     2101 aacctaatca ctgattacga ctcgaaacct ttgcccgttc gtcttatgtg tcttttgtgc
     2161 ctttttgtgg catgctgaac gccgatggac ctgaacgaac tggcgaagct tttagcagct
     2221 tacaagagcg gcgaactcga aacaaacgag gcggtcgacc gcatcaagaa ccttcacttc
     2281 gaagacatcg gtttcgcccg cgtcgaccac tcgcgcgcgt cgcgacaggg tttcccggaa
     2341 gtcgttttcg gcggcggcaa gacgcgcgcc cagatcgtca gtatcgtcga tcgactcgtc
     2401 caacgttcac caaacatcct cgtcacacgc accgatgaaa gcacattcgg cgagattcga
     2461 aatgtggtca cagacgccga atggcacgaa gccgctcgac tcgtacgcat ccgtcgcgac
     2521 aagacgaatc atggtgtcgg ctcgattgca gtcgtgaccg cgggcacgag cgacatcccc
     2581 gtcgcggaag aagctgccct gaccgccgag acgatgggca acacggtgga ccggatctgg
     2641 gatgccggtg tcgccggaat tcatcgcgta cttgccgagc gcagcctgtt gcagcgctcg
     2701 cgcgtcgtca tcgtcgtcgc cggaatggaa ggcgcgttgc cttcggttgt cggtggtctt
     2761 gtcggcgttc cagtggtggc tgtgccaacc agcatcgggt atggcgcatc ttttggaggg
     2821 gtcgcggcct tgctcggcat gttgaactcc tgcgcgtcga acgtgaccgt ggtgaatatc
     2881 gacaacggct tcggcgcagg atttgtcgcg agcctcatca atcgtcgctg gacggtgccc
     2941 gttgaataga ccagcagtcg cgtattctcg tcgtttaatc ttcgttctga ttgccgtctc
     3001 gctggtttcg agcggctgca tctccgcgcg tcgcactccc aatctcgaac agatcttcgc
     3061 caccgcgcgc gcccggaccg gaaagcgtcc ggtgatcgtg attcccggaa ttctcggaac
     3121 ggaactgatg aatccgaaga cgggcgaaat agtttggccc tccgcgtttc gcacgtcgca
     3181 ggaaggactg ccgattagtc ctgatctcgc ggctaatcgt gacgatctca taccgggaaa
     3241 gatcatcgag acggtgaaac tcgcgcgcgt gctgcccgag gtgtacgtct atcgagactt
     3301 gctggtggcg ctgcgtcgct acgccggcta tcgcgatggc gactggaaca atcccgctgc
     3361 tgatggctac gaggacacgt tctacgtttt tccttacgac tggcgacagg acaacgtcag
     3421 caatgcacgt gagctcatca gacgtatctc gcaactgaag acgaaactgc aacgtcctga
     3481 tttgaagttc aatatcgtgg cgcactcgat gggcggcttg attgcgcgtt acgctgcgat
     3541 gtatggcgac gctgatttac cacccggcaa tggtccaatt caaccgacgt ggcagggcgc
     3601 ggcggacatc agcaagatcg tgatgattgg cgtgccgaac gaaggctcaa ctgacgcgtt
     3661 cgcgacgctc gtcgagggtt actcgatcac ggagggattg cgccgtcgtg tgccgctgct
     3721 taataagttg acggcggaag atgtcgtgag cgcgccggcg gtttttcaac tcatgccgca
     3781 cgcgcgcgta gtgcggtttc tcgacgagaa tctgcaaccg ctcgcggtgg atctctacga
     3841 tccggaggtt tggaaacggt atggctggag cgtgatctat tcgtctcccg attttagacg
     3901 acaacatgct ggcgatctcg acgcatatct cactgcgact ctgcaacgcg cgcgtcgttt
     3961 tcatgaagca ctggatgcga tgcgccccga agacacgcca gtgattctgc ttgcgattgg
     4021 tggtgattgt gaagagacgc tgagctcgcc agtgattgtt cgcgatcaaa aacgaaatcg
     4081 ctgggtaacc ttgacgagac cgcgcgagtt tcgcacgtcc agcggcgtaa agatctcgaa
     4141 acagaaggct actgatgcga tgtacgcgcc cggcgacggc cgcgtcacgc gccagtcatt
     4201 gctcggggaa aatattttca aatcgctctc gcgctacgcc atcttcggct gcgatctcca
     4261 tggtcaactt ccacgcaacc caagtctcca ggacaacgcc ctcaccgcca tagtcaacga
     4321 agtgatcaat tagccacgga taacacagat ttctttctga tccgcgttaa tcgtttttag
     4381 gatccgtcta atccgcggtc acggattgat gatcgcgttc agcttttccg cgtaatcagg
     4441 atcgatgttg gtgaggatcg tgtactgctc gagcgcggcg tcgcggttgc ccagcgccat
     4501 cagcgtccgc ccgaggttgt agtaagcctt cccgaaatcc ggcttcaaac gaatcgactg
     4561 ccgaaacgat tccaccgcct cattcaactt gttcaactcg aaatatgcct cgcccatgtt
     4621 gttcggcgcg ttcgcgtcac ccggcttcaa agccgcaagc tgtcggaaga cctcgaccgc
     4681 ttcgctgtac ttcttcgact tcaaataacg tgacccgagt cgctcgtacg caacagtgta
     4741 atccggtcgc aatcgaatcg cctgcttgta ggcctggatc tcatcgtcgg gcttgcccca
     4801 atcgcgataa acaagaccga gcgcgtattg agcgtcggcg gcgttgtacg gatcgagttt
     4861 gatcgactgg cgataaccgt tctcagcgtc tttatattgc ttgagataaa aactcgcgag
     4921 gccgatgttg aaatacgact ccgcagatgg tcgcaagcta acggccttct tcgaagcctc
     4981 gagcgcttca gcatgccggc cgagtttctc attacagaaa cccgtctgcg cccatgcttc
     5041 agcgtagttg ctgtctgact caaccgcctt ctcgaaatac gggagggcct tctcgcaatc
     5101 gtccttagaa agaaaactca aaccatcgcg gaaaaactgg accgccttcg cgcgcttgtt
     5161 acggcccgtg ctcgcgacga gatccgcgag cgacgacatc gtttgggtct gcgcgacttg
     5221 catttgtgaa atgcgttcgg agggaatcgc gaagttgaca ctttgcccgc cggtgatctg
     5281 caaagtggcg atgccgatca cctggccctg catgttgacg accggactgc cgctcgatcc
     5341 cgaagagatc ggcgcggtga tctgaatgat gcggccgaac gtcggaatgt cgcgcaccgc
     5401 cgatacgatt ccatttgtga cgctgccttc gagtccgagc gggttgccaa tcacgaccac
     5461 gctctcgcct tcctgtggcg aggttttgtc gagtggcaac gggcggactg gcggcgccgg
     5521 cgcgtcaatc ttcaagagcg cgatgtcgcc ctcagcatcg accgcaagca cgcctttgac
     5581 agggaagact gccccactcg atgagtgaac ttccgcgcga tatgcgcctt cgaggacgtg
     5641 gcgattcgtg acgatgcgat cggcctcaac aaagaagccg ctgccgcgag acagtttttc
     5701 acccttcgag tcgaacgtct cgatcgcaac cgcggacggc ttgatgcgac gaacgagttc
     5761 gggaagaaga tcctgcgatg cctcgatggg gtggggaatt agcaggaaaa gtagaatcca
     5821 gacgaacttc atgtcggaga aaaattttta cacgtgtctc agcctggcca cgacgtcccc
     5881 gattctaaca tcctcgctct tctttgcgct ccgatcgaac aactcaacca tgccgtcggc
     5941 gatttttttt ccaatcgtga tccgataagg gatgccgatc agatccgcat ctttgaattt
     6001 tacacccgcg cgttcgtcgc gatcgtcgag caagacgtcg aaaccggctc cttgcaattc
     6061 ctcgtacagc ttctcgccag cattacgcag gtcgtcctgc ttcatgttcg tgatcgtcac
     6121 gacaacatca aacggcgcca gcgacttcgg ccagatgatg ccgtcgtcgt cgtggtgttg
     6181 ctcgactgcg gcggtgatga ttcgttccac gccgataccg taactgccca tcacgattgg
     6241 cacttctttg ccgtcctgcg tcagcacggt agcacccatc gaagcggaat atttcgtacc
     6301 gagcttgaag atgtgaccga tctccatgcc tttgaaaatt tcgagcgtgc cggtctcgca
     6361 gcgtgggcat ccctcgccgg cttcaaccgt gcgcagctcg aaccactcgt caacaggaat
     6421 gtcacgtgtg atatcgacgc cacgcaggtg gtgatcgtct ttgttggcgc cggtggtcat
     6481 gttccggcga ttctgcaacg cggaatcagc cacgattcgc aagtcacgcg atctattctt
     6541 tgcaccgacg ccgccgagac tgccggcgct cgcgcccagc aagtcgcgaa tctcttcggg
     6601 atgagcggga cggacggctg tcgcacgaat actgtcgctt agcctcgtct cgtgaagctg
     6661 gtgatctccg cgcaacaaca ccaacaccgg acgcgactcg ccatcgatcg tcgcgatgaa
     6721 aaccagcgac ttgatctggc ggtttgcgtc agcgccaccg ggaaacgtaa tcagatcctc
     6781 gatcgtgcga actcccggcg tcggaaattc ttccggcgct gctggtccag gctcgtctgc
     6841 aatttgtgcc agtcgcgagg tggctttctc gaggttggcg gcgtaatcgc agttagtaca
     6901 actgacaatc acatcctcgc ccgcgggtga tggcgccatg aactcattcg actccgatcc
     6961 gcccatcgag cccgacgacg cttcaacaat caaaaactgt aacccgcaac gagtgaagat
     7021 cgtcttgtaa gcttcacgct gatcgataaa cgacttgtcg agcccggcgc gatcgacgtc
     7081 aaacgtgtat gcgtctttca tgatgaaggt acggattcgc atcagtcctg atttgggccg
     7141 tgcttcatcg cgaaacttgg tttggatctg ataccagacc tgcggcagtt gtttgtacga
     7201 acgaagctcg ctgcgcgcga tcgtcgtgaa gacttcttca tgcgtcatgc ccaggcacat
     7261 gtcggtgccc ttgcgatcct tgagacgaaa catctcgtcg ccgatcgcgt accaacggcc
     7321 cgactcctgc cagatctcag ccggatgaag cgcagggaga aaaaactcct gaccgccaat
     7381 cgcgttcatc tcttcacgca ggatctgcat gactttgatt gcgactcgct ggcccaacgg
     7441 caggaaagaa tagattccag ccgacaattg ccggatcagc ccggcgcgca atagaagctt
     7501 gtgcgaaatg acctcggcgt ccgcggggtc ttcgcgaaga gtgggaatga agtatcgtga
     7561 ccagcgcata gtcttggcat tctcgcttag tgaacgcgag ttaggcaaat gaggctcatc
     7621 cacagattac gcagatttta taaattcgtc tgtgtaatct gtggataaaa tgggttacca
     7681 tcacggcttc ttcttaacat tactccacga aggttcttga tatatgcgta ttgcactcgc
     7741 aagcgatcat gctggttacg ccgagaagga gcgtttgaag ccgctgctgg cggacctcgg
     7801 cgttgagttt gacgatctcg gcaccatgtc ggaagagtcg gttgactatc ctgactatgc
     7861 gcgcaaggtt gcggagcag
//
LOCUS       DASSFQ010000251         9889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_116630, whole genome shotgun sequence.
ACCESSION   DASSFQ010000251 DASSFQ010000000
VERSION     DASSFQ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116630"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFH96_09120"
     CDS             <1..695
                     /locus_tag="VFH96_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974171.1"
                     /translation="ARRGYGVTYSTLRDQQLLAEVARASQMFESLQSAEMPSNLQKSE
                     RHLIPRQFAWTRDDVDAATLPQMIVREDKTIIYQFKPQMASAERFEKIGEGSGYIWGS
                     GSGYLEYTVPTRADRRKVSEIIVRAHIQPVLPVDASTDFVKTRVTLFVEDWDGGSRLI
                     PVEPKGQPIVQEWRLTGLFLRLRAMRGLPLKIRFEVTPESDWLYGVNISNWPEGYDSK
                     DTRPVEVELRHD"
     gene            complement(755..2401)
                     /locus_tag="VFH96_09125"
     CDS             complement(755..2401)
                     /locus_tag="VFH96_09125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6974172.1"
                     /translation="MSVHAEAIITAGLRPDLFAGRGVLAHSKTLVFAALIVLVVAGFG
                     FRATGLSAEGLSEDELNKLNAVADYRAHGLTGANGEHPMLMKACQAVSIILAEKWNNT
                     FASNQIAPETALRLPNAIVGALTTILIFLIASELFGLEVALIAAALWAFDPSAIGLNR
                     IAKEDSFLVFFFLLANVFWLRGQRAAESTDSSPNKYYWLTAASYGAMVASKYVPHLFA
                     ISICYYWMFQSLPETRWRLGKKRLLIFFTIMGLVFLVLSPTVLLPETWRQMGLFAGGK
                     RVSHDGYEFMNQLYTQRFSDWLNGIPVYFYVVFTAVKLPVLTVLGFVAGLPLLFRRKL
                     GDGRYFILFWFLLWVAAFCFPGGKFTRYYTTILPAVLITSALGIQFVGRWLANRISDG
                     ASLKHYIPATLAVIVIAGSVIDSVNAAPHFRLFLNSIGGGTNWAGYYFPHDEFYDASM
                     RDAMAEIATRARSGARVASESPSLATYYAERAKRPDLVCVSLSDPDAVKQLAVGDYVI
                     VARGRRYFSNDSIISTLRDHATPIAELKLGLVPSAKIYEY"
     gene            2561..3868
                     /locus_tag="VFH96_09130"
     CDS             2561..3868
                     /locus_tag="VFH96_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6974173.1"
                     /translation="MDQTQLARRLGLFDATMIVMGGIIGSGIFMNPSVVALQVHTPFL
                     ILGAWAVGGLFALTAAFIWAELASLRPDVGGQYAYLREAFHPGVAFLYGWVLLLVIQT
                     GGMAAVAVTFSRYFVELTNVPISSAYLAAIVLATLTAINCLGVRAGSTVQSILMVLKI
                     VAIAMLVVCGFLFAQRSANPSGLLDRPVSGDLITAFGAALVPVIFAYGGWQTSTFIAG
                     EIKEPRKNLPRGLILGVTGVVVLYLAANVVYTSVLGTAALAASKAPASDVMRSALGDF
                     GARLIAAGIAISTAGFLSQSMLTAPRVYFAMAKDKLFFERVGMVHPQTRAPIVAIALQ
                     GLLAIVIALLGTYERILNYVVSIDMIFFGLTACCIFIFRRREGPGVTRVPGHPFTTIV
                     FIAICWLVVINTVYRYPENTLIGIAIMIAGIPAYWFWRQRSSR"
     gene            3865..4944
                     /locus_tag="VFH96_09135"
     CDS             3865..4944
                     /locus_tag="VFH96_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6974174.1"
                     /translation="MTAAREVMGSAYMTWAKTRSVAKYNLATSGLGNVKLSEIRVSLD
                     DLEITDGGYGYQPLIQSIATRYRVAPEEVVTAAGTTFANHLAMAALIKPGDEILFEHP
                     AYEPMLALAGYLGADVKRFSRRFEDDFQPEDLYDRITPKTRLIVITNLHNPSGVLIDD
                     TRMKQIGEIARRVGARVLVDEVYMETLFEELPRTSFHLGEEFVVTSSLTKAFGLSGLR
                     CGWILAQPDLAKRMWLLNDLFAATPVHAGERLSVVAMQQLEGIGERAKHLLDRNRQIL
                     NEFLDTREDLEVVRPEFGTVMFPRVRHGTSESLCELLREKYETSVVPGVFFEMPAHFR
                     LGIAGDTDLLLEGLTRLGLALDEHG"
     gene            complement(4971..5624)
                     /locus_tag="VFH96_09140"
     CDS             complement(4971..5624)
                     /locus_tag="VFH96_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974175.1"
                     /translation="MKMLPGFQRNRHKKGLFAASSLLLLLATQTVAQSGRRAPPKPDP
                     TPFPTPQTDAAVVPKSNARELKQRVSLLVGIEPSSKHLLSEDAIFANFLQHLGEFKNV
                     KTTSMGDLKRDRAVKRAKSETESIVVLLQFDVDEFQSGTVILNSPDLDVKVLVFEPKT
                     GQKKFEGKVYYKAVGGPMLKKDNWPTGTPIRITTEAVGIEAAEQVRDWLLVQNINHR"
     gene            complement(5670..6632)
                     /locus_tag="VFH96_09145"
     CDS             complement(5670..6632)
                     /locus_tag="VFH96_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974176.1"
                     /translation="MIGHAFLLLLLYTTLFQGPNCGCEQKPEINVLAVVNGVKITRKD
                     LSIDTLTQTSIAQDTVIVARTRQLELKINDLLLEMEAKRRGITTAELLETGVTSKVTP
                     PTEAEAKAIYEQNNDRSQSFNSVKNHIMAQLRTERVAARVREFANTLRAGAKITIANQ
                     LVTPPTNEAELARVFAIVNGVNITSSDIETALLPLIFQVQQQVYALRKRDVDLRINDL
                     LLEQEAKKLGTTPQALIDSHVITKMQIVTEEQARAYYNANKAQLKASFSEAKAMIMQY
                     LLELEKRKAQVAYAEQLRKESAVQIYLTEPKPTNVRQLCCNLVG"
     gene            complement(6708..7682)
                     /gene="hemB"
                     /locus_tag="VFH96_09150"
     CDS             complement(6708..7682)
                     /gene="hemB"
                     /locus_tag="VFH96_09150"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET6974177.1"
                     /translation="MIHRPRRLRRTDAIRSLVRETRLAPENFVLPLFVCPGTGVRREI
                     SSMPGVFNLSIDELTKDVAAAQDTGVRSIILFGLPESKDAQASGAYAEDGIVQRAIRA
                     VRQSSPEMIIMADTCLCEYTSHGHCGVVRGGEVINDESLDLIARTALSQAAAGADIVA
                     PSAMMDGQVETIRTLLDSEGFENTAIMAYAVKYASGFYGPFREAAQSAPEFGDRRAYQ
                     MDPPNVREAMREAELDYAEGADILMVKPATVYLDVLKMVRERFDVPCAAYHVSGEYAL
                     IKAAAQKGWIDEERVMMETLTSIKRAGADIILTYYAREAIRALMSDKL"
     gene            complement(7698..8156)
                     /locus_tag="VFH96_09155"
     CDS             complement(7698..8156)
                     /locus_tag="VFH96_09155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6974178.1"
                     /translation="MNSIQETFAPNLACFGCGPANPKGLHVRSFPDGEEVVAEWQPSK
                     EYEAFEGMLSGGIIGTLLDCHCNWSAAWHLMQKSGADKPPCTVTAEYSIKLLRPTPSD
                     RPVKLVARVVESTNDKAIVEGELIAHDKVCATCRGTFVAVKPGHPAYHRW"
     gene            complement(8153..9721)
                     /locus_tag="VFH96_09160"
     CDS             complement(8153..9721)
                     /locus_tag="VFH96_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET6974179.1"
                     /translation="MNLFNAIFRNDSSSPAIRFDGRTITYGELRAETSTMAQVIKSLG
                     VASGDRVALLLHDSPEFVEAFIATISLGAIAVPINMVLRAGEQCSILHSSGASLAFIE
                     AETCRTLLTHAPEKLQSLKNAVVLERAGGRACYERLGSITLHSLELLRDKAAGTVEFP
                     VAGPTDPAFILYTSGSTGEPKGAVHTQDHIIYTNETFCREVLCLETGDRLFSSSRLPF
                     AYGLGNSFTFPLLNGATAILCREKPSPDIVSRMFNEHRPTIFFAVPVVYNLLLEHHRA
                     GMKLDCSSLRLCVSAGEALPAQLGEEWEKEFGVQLLDGIGSTEMLHMFMSNYENEVRY
                     GSSGRLIKGYEARLVDEHGEPTPVNTEGHLWIKGESAALCYWENPEATANTFVDGWVR
                     TGDLYRCDSDGYWYHMGRSDDCFKSSGRWVSPVEVEGALLRHDGVARAAVVEDFDSNQ
                     LPCACAFVVKQDVESDSAQLEHELRKLAAQSLARFKQPRRYVFVAELPYTATGKIQRF
                     RLRQEIKSVKSVDK"
BASE COUNT         2150 a         2749 c         2770 g         2220 t
ORIGIN      
        1 atgcgcgtag aggttacggt gtgacttatt ccacgcttcg cgatcagcag ttgcttgctg
       61 aagttgcacg cgcgtcgcag atgttcgagt cgttgcagtc ggcggagatg ccgtcgaact
      121 tgcagaagag tgagcgacac ctgattccga gacagtttgc gtggacgcgt gatgacgttg
      181 acgcggcgac gttgccgcag atgatcgtca gggaagacaa gacaatcatt tatcagttca
      241 agccacagat ggcgagtgct gagcgtttcg agaagattgg cgagggctcg ggttacatct
      301 ggggatccgg ttccggttat ctcgaataca ctgtgcccac gcgcgctgac cggcgaaagg
      361 tcagcgagat catcgtgcgc gcgcatattc aaccggtgtt gccggtcgat gcgtcaactg
      421 atttcgtgaa gacgcgtgtg acgctgtttg tagaagattg ggacggcggc tcgcgactga
      481 ttccggtcga gccgaaagga caaccgatcg ttcaggagtg gcgtttgacc ggtctgttcc
      541 tgcgcctccg cgccatgcgt ggtctaccct tgaagattcg cttcgaagtg acacccgaat
      601 cagactggct ctacggcgtc aacatctcaa actggcccga aggctacgac tccaaagaca
      661 cccgccccgt cgaagtcgag ttaagacacg attagccaca aaaaggcaca aaagaactga
      721 acaaacattt tgtgcttttt gtgccttttt gtggctaata ctcgtagatt ttggctgatg
      781 gcacgaggcc gagtttgagt tcggcaatgg gggtggcgtg gtcgcggagg gtggagatga
      841 tcgaatcgtt gctgaagtaa cgacgaccgc gggcgacgat cacgtaatcg ccaaccgcga
      901 gttgctttac tgcgtccgga tcggaaagtg agacgcaaac aagatccggt cgcttcgcac
      961 gctctgcata atatgtcgcc agcgaaggac tctcgcttgc tacacgcgca ccgcttcgag
     1021 cgcgagttgc aatctcggcc atcgcatcgc gcatgctcgc gtcgtaaaac tcatcgtgcg
     1081 ggaagtaata accggcccag ttcgtgccac caccaatcga gtttaggaac aggcgaaagt
     1141 gtggcgcagc gtttacggaa tcgattactg agcccgcgat tacgatcacg gctagagttg
     1201 ccggaatgta atgcttcaaa ctcgcgccat cagaaatccg gttcgcgagc cagcgaccaa
     1261 cgaattgaat accgagcgcg ctcgtaatca acaccgccgg caaaatggtc gtgtagtaac
     1321 gggtaaactt tccgcccgga aaacagaacg ccgccaccca caataaaaac cagaagagaa
     1381 tgaaataacg cccgtcgccg agcttgcgtc gaaagagcag cggcagtcct gctacaaatc
     1441 cgagcacagt caacacgggc aacttcaccg cggtaaagac aacgtagaag taaaccggaa
     1501 ttccgttcaa ccagtcacta aaacgctgcg tgtagagctg gttcatgaat tcgtaaccgt
     1561 cgtgactgac gcgcttgccg cccgcaaaca atcccatctg ccgccacgtt tccggtagca
     1621 gcactgtcgg actcaggacc agaaacacga gccccatgat cgtgaagaaa atgagtaagc
     1681 gtttcttgcc gagacgccag cgcgtttccg gcaggctctg aaacatccag tagtagcaaa
     1741 tgctgatcgc gaagagatgc ggtacatact tcgacgcgac catcgcaccg tacgacgcgg
     1801 cggtgagcca gtaatattta ttcgggctgc tgtctgtgct ctcagcggcg cgctgtccac
     1861 gcaaccaaaa cacattcgcc aacaggaaga agaacaccaa aaaagagtct tctttagcga
     1921 tgcgattgag cccgatcgcc gaagggtcga aggcccacaa agcggccgcg atcagtgcga
     1981 cttccaagcc aaacagttcc gaagcgatca ggaagatcag aatcgtagtt agcgcgccga
     2041 cgatcgcatt aggcagtcgt aatgccgtct cgggcgcgat ctggttagag gcgaatgtgt
     2101 tgttccactt ttcagcgagg atgattgata ccgcctggca cgccttcatc agcatcggat
     2161 gttcgccgtt cgcgcctgtt aggccgtgag cgcggtagtc ggcgacggca ttgagcttgt
     2221 tgagctcatc ctcgctgaga ccttcggcgc tgaggccggt agcgcggaaa ccaaaaccag
     2281 cgacgacgag aacgatgagt gccgcgaaaa cgagtgtttt gctgtgtgcc aggacaccgc
     2341 gtccggcgaa gagatcaggc ctcagaccgg cagtaataat ggcttcagca tgtacagaca
     2401 agattcaaac ctctcaacaa aatgcacaag gtatcggaag agtcgcgtgc tgtcaaaaac
     2461 aggttagatg cttatccaca gattacgcag attacacaga ttttagaaac cgctctgtgt
     2521 aatctgcgta atctgcggat agaatggtaa tcctgtcccc atggaccaaa cacaactagc
     2581 gcgcagactc ggcctcttcg acgccaccat gatcgtgatg ggcggcatca tcggctcggg
     2641 catcttcatg aacccgtcgg tggtcgcact ccaggtccac acgccgtttt tgattctcgg
     2701 cgcgtgggcg gtcggcggat tgtttgcact gacagccgcc ttcatctggg ccgagcttgc
     2761 ttcgctgcga ccggacgtcg gcggccagta tgcttacctg cgcgaagcgt ttcatcccgg
     2821 cgtggcgttt ctttacggtt gggtcttgct actcgtgatt caaaccggtg ggatggccgc
     2881 agtcgcagtc accttctcgc gttactttgt cgagctgaca aatgtgccga tctcatccgc
     2941 gtatctggct gccatcgtgc tcgccacatt gactgcgatc aactgtctgg gtgtgcgcgc
     3001 aggcagcact gtgcaaagca tactgatggt gttgaagatc gtggcgatcg cgatgctggt
     3061 ggtgtgcggg tttctgtttg ctcagcgaag cgcgaatcct tccgggctct tggatagacc
     3121 agtctcaggc gatttgataa cggcattcgg cgctgcgctc gtcccggtga tctttgctta
     3181 tggtggctgg caaacatcga cgttcatcgc cggtgagatc aaagagccac gcaaaaatct
     3241 tccgcgcgga ttaatactcg gcgtcacggg tgtggtcgtg ctctatctag ccgcgaacgt
     3301 ggtctacacg agcgttctgg gtaccgcggc gctcgccgcc tcaaaagcgc ccgcgtctga
     3361 cgtgatgcgc agcgcactcg gagacttcgg cgcgcgtctc atcgcggcgg gcatcgcgat
     3421 ctcgaccgca ggctttctta gccaaagcat gttgactgcg ccgcgagttt acttcgcgat
     3481 ggcaaaagac aaactgttct tcgagcgcgt cggcatggtc catccacaaa cacgcgcgcc
     3541 gatcgtggcc atcgcgcttc agggtctgct ggcgatcgtg atcgctctcc tcggaactta
     3601 cgagcgcatc ctgaactacg tcgtctccat cgacatgatt ttctttggac tcaccgcgtg
     3661 ctgtattttt atctttcgta gacgcgaagg gcctggcgtg acgcgagttc ccggtcaccc
     3721 gttcacgacg atcgtcttta tcgcaatctg ttggctcgtg gttatcaata cggtctatcg
     3781 ttacccggaa aatacattga taggaatcgc aatcatgatc gcaggtattc ccgcttactg
     3841 gttttggcga cagaggagca gcagatgacc gcagcacgtg aagtgatggg ttcggcatac
     3901 atgacctggg ccaagacgcg ctccgtagcg aaatacaatc tcgcgacgag cggtctcggc
     3961 aatgtaaagc taagcgagat tcgtgtttcg ctcgacgatc tcgagatcac tgacggcggc
     4021 tacggttatc aacccttaat acagtcgatt gcaacaagat atcgagtcgc tcctgaagaa
     4081 gtagtgacgg cagcggggac gacgttcgcg aatcacctcg caatggcggc gttgatcaag
     4141 cccggagatg aaatcttatt tgaacatccg gcctacgagc cgatgctcgc gctcgctggt
     4201 tatctcggcg cagacgtcaa acgcttctcg cgccggtttg aggacgactt ccagccggaa
     4261 gatctctacg accggattac tcctaaaact cgactgatcg tgatcacgaa tcttcacaat
     4321 ccgagcggtg tattgatcga cgatacgagg atgaagcaga tcggcgagat cgcacgtcgc
     4381 gtcggtgcgc gcgtgttggt tgatgaagtc tacatggaaa cgttgtttga agagttgccg
     4441 cgtacgagtt ttcatcttgg tgaagagttt gtagtgacga gtagtttaac gaaggctttc
     4501 ggattgagcg gcctgcgatg tggttggatc ctcgcgcaac ccgatctcgc gaaacgcatg
     4561 tggctgctca acgatctctt cgcagcgacg ccggtacatg ccggtgagcg gctgagtgtg
     4621 gtggcgatgc aacagctcga aggtattggc gaacgtgcaa agcatctgct cgatcgaaat
     4681 cgtcagatac tgaatgagtt tctcgacacg cgtgaagatc tcgaagtggt caggccggaa
     4741 tttggaactg taatgttccc gcgcgtgcgt cacggaacat ctgaaagcct ctgtgaatta
     4801 ttgcgtgaaa aatacgagac gtcagtcgtg cccggagtct tcttcgaaat gcccgcacac
     4861 ttccggctcg gcatcgcagg tgacacggat ttattattag aaggcctaac ccgattagga
     4921 ctcgcactgg atgagcacgg ataaacgcag atcggtttaa aaaatccgtg tcatctgtgg
     4981 ttaatgttct gaactaaaag ccagtcgcgc acctgctcgg cggcttcaat cccgacggct
     5041 tcggtcgtga tccggatcgg cgtgcccgtg ggccagttgt ctttcttgag catcggacca
     5101 ccgaccgcct tgtaataaac cttcccctca aacttcttct gccccgtctt cggctcgaac
     5161 acgagcacct taacatcgag atccggtgag ttcagtatga ccgttccgct ctgaaactca
     5221 tcaacgtcaa actgcaacaa cacaacgatc gactccgtct cgctcttcgc tcgcttcacg
     5281 gcgcggtcgc gtttgaggtc gcccattgaa gtcgtcttga cgttcttaaa ctcgccgagg
     5341 tgctggagaa agttggcgaa gatcgcgtct tcactgagca ggtgttttga cgaaggctca
     5401 atcccaacca gcagcgacac acgctgtttc aactcgcgcg cattgctctt cggcacgact
     5461 gcagcgtcgg tttgtggcgt tgggaaaggc gttggatcgg gcttcggagg ggcgcgcctg
     5521 cctgactgcg ccacggtctg cgtggcgagc agaagcagca ggctcgatgc tgcaaaaagg
     5581 ccttttttgt ggcggtttcg ctgaaagccg gggagcattt tcatgcgcta ttctccagcg
     5641 gtgaccagaa tgtgacctta gaagcgggct tagcctacaa ggttgcaaca gagttgtcga
     5701 acattcgtcg gcttcggctc ggtcaggtag atttgcacgg ccgactcctt ccgcaactgc
     5761 tccgcatacg ccacctgcgc ctttcgtttt tccagttcca acagatactg catgatcatc
     5821 gccttcgcct ctgaaaagct ggctttgagt tgcgctttgt tcgcgttgta gtaggcgcgc
     5881 gcctgctcct cggtgacgat ctgcatcttt gtgatgacgt ggctgtcgat gagcgcttgc
     5941 ggcgtcgtac caagcttctt ggcttcctgt tcgagcagca gatcgttgat gcgcagatcg
     6001 acatcgcgct tgcgcagggc atagacctgt tgttggacct ggaagatcag cggcagcagg
     6061 gcagtctcga tgtcgctgga agtgatgttc actccgttga cgatagcaaa cacgcgcgcg
     6121 agctctgctt cattcgtggg cggcgtgact agctggtttg caatcgtgat cttcgctccc
     6181 gcgcgtagag tgtttgcgaa ctctcgtaca cgcgctgcca cgcgctccgt tctcaactgc
     6241 gccatgatgt ggtttttgac gctattgaaa ctctgactgc ggtcattgtt ttgttcgtag
     6301 atcgctttcg cttcagcttc ggttggtggc gtgaccttcg aagtaactcc ggtttcgagg
     6361 agctcggcag tcgtgatgcc gcgacgcttg gcttccatct cgagcagaag atcgttgatc
     6421 ttcaactcga gttgccgggt gcgcgcgacg atcactgtgt cctgcgcgat gctggtttgt
     6481 gtgagcgtgt cgatacttag atctttgcgt gtgatcttga cgccgttgac tacagcgagg
     6541 acgttgatct cgggtttttg ctcgcagccg cagttcgggc cttgaaaaag agttgtgtac
     6601 agtaacagca agaggaaggc atggccgatc atatcaaccg ttaggtagtg gttcactttc
     6661 gctgcaagtg tccgataaac tttagtttgt cgcgacattc cgacaagcta aagcttatcg
     6721 gacatcaaag cacggatcgc ttcacgcgcg tagtacgtca ggatgatgtc ggcgccggcg
     6781 cgtttgatgc tcgtgagtgt ttccatcatc acgcgctctt cgtcgatcca gcctttctgc
     6841 gctgccgctt tgatcagcgc gtactcgccg gagacgtgat acgccgcgca cggaacatcg
     6901 aatcgctcac gcaccatctt caacacgtcg agataaaccg tggcaggctt caccatcagg
     6961 atgtctgcac cttcggcgta gtcgagctcg gcctcacgca ttgcctcgcg cacattcggc
     7021 ggatccatct ggtaagcgcg gcggtcgccg aattcgggcg cgctctgtgc cgcctcgcga
     7081 aatggaccat agaaccctga cgcatacttc accgcatacg ccatgatcgc cgtgttctcg
     7141 aatccctcgg agtcaagtaa ggtgcgaatg gtttcgacct ggccatccat catcgccgag
     7201 ggggcgacga tgtccgcgcc cgcagcagcc tggctcaacg ccgtgcgcgc gattagatcg
     7261 agtgattcat cgttgataac ttcaccaccg cgtaccacac cacaatgacc gtgcgatgtg
     7321 tactcgcaca ggcacgtgtc agccatgatg atcatttcgg gcgacgactg tcgcaccgcg
     7381 cgaatcgcac gctgcacgat tccgtcttcc gcgtaagctc cggacgcctg cgcatctttc
     7441 gactcaggca gaccaaaaag aatgatgctg cgcacgcccg tgtcctgtgc ggcagcgacg
     7501 tcttttgtca gctcatcgat cgagagattg aagacgccgg gcatcgacga gatttcgcga
     7561 cgcacgcctg tcccgggaca gacaaacaac ggcaacacga aattttccgg cgcgagccgt
     7621 gtctcgcgta cgagtgaacg gatcgcgtca gttctgcgaa gtcgacgagg gcgatgaatc
     7681 atgtacgaca tttttattta ccaacggtga tacgcgggat gtccgggttt gacggcgacg
     7741 aaagtgccgc gacaagtggc gcagactttg tcgtgcgcga tcagctcgcc ttcaacgatt
     7801 gctttgtcat tagttgactc gaccacacgc gcgacgagtt tcactggacg atcgctcggc
     7861 gtcgggcgca agagcttgat cgaatactca gccgtgacag tacagggcgg tttgtcggcg
     7921 ccgctcttct gcatcaggtg ccacgcagcc gaccagttgc agtgacaatc gaggagtgtg
     7981 ccgatgatgc cgccgctcag catcccctca aacgcttcat actccttgct cggctgccac
     8041 tccgcgacaa cttcttcgcc gtcgggaaag ctgcggacgt gcaggccttt cggattcgcc
     8101 ggcccacagc cgaaacacgc gagattcggg gcaaatgttt cctgaatact attcatttat
     8161 ccacagattt cactgattta atttcctgcc gcagcctgaa ccgctgaatt tttcccgtcg
     8221 ccgtgtacgg cagctcggcg acgaacacgt accggcgcgg ttgtttgaat cgcgccagcg
     8281 actgagccgc gagcttgcgc aattcgtgct cgagttgcgc cgagtcggat tctacatcct
     8341 gcttcacaac aaacgcacac gcacacggaa gctgattgga gtcaaaatct tcaaccacag
     8401 ccgcgcgcgc cacaccgtcg tgccgcaaca acgcgccctc gacttcaact ggcgagaccc
     8461 agcgcccact cgatttaaaa caatcatcgc tgcgtcccat gtgataccag tagccgtcgc
     8521 tgtcgcaacg atacagatcg ccggtgcgga cccagccgtc gacgaatgtg ttcgcagtcg
     8581 cctcgggatt ttcccagtaa cacagcgccg cgctctcgcc tttgatccag aggtgtcctt
     8641 ctgtattcac cggcgttggc tcgccatgct cgtcaaccag acgtgcttca tagcctttga
     8701 tcagacgtcc actgctgccg tagcgcacct cgttttcata gtttgacatg aacatatgca
     8761 gcatctcggt cgatccgatg ccgtcgagca gttgcacgcc aaactccttt tcccactctt
     8821 caccaagctg agcgggcagc gcttcaccag cggagacgca gagccgcaga ctcgaacagt
     8881 cgagcttcat tcccgcgcga tgatgttcga gcaaaagatt gtagaccacg ggaacggcga
     8941 aaaagattgt cggtcgatgc tcattgaaca tccgcgagac gatatcgggc gacggctttt
     9001 cgcgacagag aatcgctgtt gcgccgttga gaagtggaaa cgtgaagctg tttccgagac
     9061 cgtacgcgaa cggcaatcgc gaagacgaga acagccgatc gccagtctcg agacacaaca
     9121 cttcgcgaca gaaggtttcg tttgtgtaga tgatgtggtc ctgcgtgtga acggcgcctt
     9181 tcggctcgcc cgtgctgccc gaggtataga gtatgaatgc cggatcggtt ggaccagcaa
     9241 ccggaaactc gacggtcccc gcagctttat cgcgcaaaag ctcgagcgaa tgaagagtta
     9301 tcgagcccag gcgctcgtaa caagcccgcc cgcccgcacg ctccaacacg accgcatttt
     9361 tcagtgactg caacttttct ggagcatgtg tcagcaacgt gcggcaggtc tcggcctcga
     9421 taaaggccag actggctccg gaactgtgaa ggattgaaca ctgctctccg gcccgcagca
     9481 ccatgttgat gggaacagcg attgccccaa gcgaaatcgt ggcgatgaag gcttcgacga
     9541 actcgggtga gtcgtgaagc aggagcgcaa ctcgatcgcc gcttgcgacg ccgagcgatt
     9601 tgatgacctg ggccatcgtc gacgtctcgg cgcgcaattc tccataagtg atcgtgcggc
     9661 cgtcgaaacg gatcgctggt gacgaagagt cattccggaa gatggcgttg aagaggttca
     9721 ttagtctttt acatgatttt caagattccc aggattccca agattaaatc ctgtaaatcc
     9781 tgaaatcctg tctaacaaat gtcatggaca tcacacatca taaatcattc tcgaacgtgc
     9841 tcaactcctt catggaagag agcaacattc gctggggcga gatcgtcgg
//
LOCUS       DASSFQ010000252         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1609605, whole genome shotgun sequence.
ACCESSION   DASSFQ010000252 DASSFQ010000000
VERSION     DASSFQ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609605"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..716)
                     /locus_tag="VFH96_09165"
     CDS             complement(93..716)
                     /locus_tag="VFH96_09165"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HET6974180.1"
                     /translation="MSVLASAQAVVPPQADNQLWTDVQFAVPVTKNFDFNLLGTLRLG
                     RDISRPVDERVGVGFTFRWGKYFSASPNYLHIGMQPFRGRKVWENRLTLPATLRFTAG
                     KFRLSDRNQLERRLRNSGAKSWRYRNRFQVERQVGSIKHGLALYVADEVFYDWAVDRW
                     VRNRFSVGGIKVFNKHYTQEFYYLRQNDGVSLPGDLNVIGTTLRFRL"
     gene            complement(752..1966)
                     /locus_tag="VFH96_09170"
     CDS             complement(752..1966)
                     /locus_tag="VFH96_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974181.1"
                     /translation="MVKAKETLIPRVVEPVSNTPTPPAAASVIGISKRFGKASVLENI
                     SFDVAEGEVLVLLGASGSGKTTILRIIAGLEMPYTGKVILHGREVTELPARERGVGVI
                     FQSYALFPKMTVEKNIGYGLRIRKRKRKEIRETVNELLQLVQLEEHRKKYPSQLSGGQ
                     QQRVAIARTLAYKPEVLLFDEPFGALDTQTRVHLRREIRALLKKVNVPSIFITHDQEE
                     ALELGDRVAVLNVGHVEQIGTPFEIYNYPATEYVATFLGAANLLDAVVRSGYLEVGSA
                     QIPATVDKEKFKPGDCVKIVFRPEDVSLNRGDFLRPGLARLGNALIEEISFVGAYERL
                     RLQMDVGGADECKTDETPFYLTTQTPERQSAKSIIATRPKPEAAATRLQVGERVVVAL
                     TSFTVLPSKGAR"
     gene            complement(1976..>2587)
                     /locus_tag="VFH96_09175"
     CDS             complement(1976..>2587)
                     /locus_tag="VFH96_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6974182.1"
                     /translation="TVFNVLFGLFAAYVLSRYNFLGRNALIVTISLPTAIPTAVAGFA
                     LLLLWGPYGTLGRLLTPHGFQIMFTAGAIILANIFVTFPLAFGVIKPVFDTMSRSLEE
                     ASATIGATRWQTFWYVTLPSLRGAIVSGALLTFARAVGEFGSTILVSGNLAGRTQTAP
                     LYIFAKFNEGQIEAANAIAIVLALFSFLIFSVLFLARDWLDVD"
BASE COUNT          605 a          748 c          701 g          533 t
ORIGIN      
        1 atcaaggcac tgacataaag tttcacgcaa aggcgcaaag aagaagagcc cgttttcctt
       61 ttgcatcttt gcgcctttgc gtgaaaccag ccctataacc taaacctcaa ggtggttccg
      121 attacgttca gatccccagg caaactgacg ccgtcgttct ggcgaagata atagaactcc
      181 tgcgtgtagt gtttgttgaa gaccttaatc ccgccgactg aaaaacgatt gcggacccag
      241 cgatctaccg cccagtcgta aaacacttcg tcagcaacat acaacgccag gccgtgcttt
      301 atagaaccga cttgacgctc gacctgaaac cggttgcggt agcgccacga cttcgcgccg
      361 gagtttcgca aacgccgctc aagctgattc cgatcgctca agcgaaactt gcccgccgtg
      421 aaccgcaagg tggccggcaa agtcaggcga ttctcccaaa ccttgcgccc gcgaaacggc
      481 tgcatgccga tgtgtaagta atttggcgac gcgctgaaat acttgcccca acgaaaagtg
      541 aatccgacac cgacgcgctc atctaccggg cggctgatat cacgtcccaa acggagtgtg
      601 cccaaaagat tgaaatcgaa attcttcgtg accgggacgg cgaattgaac gtcagtccat
      661 aattgattat cggcctgtgg tgggacaacc gcctgcgctg aggcaaggac actcaacgca
      721 caaaccaaaa aagaaacgtt gacaattctt ttcaacgtgc gccctttgac ggtaagactg
      781 tgaatgacgt cagcgccacg accacacgct cgcccacctg caatcgcgtc gcggccgctt
      841 ccggcttggg acgcgtggcg atgatcgatt tggctgactg tctctcgggg gtctgcgtgg
      901 ttaggtagaa cggcgtttcg tcagtcttgc actcgtccgc gccgcccaca tccatttgta
      961 aacgcagccg ttcataagcg ccgacgaagc taatctcttc gatcagcgcg ttgccgagcc
     1021 gtgcaagacc cggccgcagg aagtcgccac gattcaacga aacgtcttcc ggtcgaaaga
     1081 cgatcttcac gcaatcgccc ggtttgaact tctccttgtc cactgtcgcg ggaatctgcg
     1141 ccgaaccgac ttctagataa ccacttcgga ccactgcatc gagcagattc gcagcgccga
     1201 gaaaagtcgc gacatactca gtcgccggat agttgtagat ctcgaatggc gttccgattt
     1261 gttcgacatg gcccacgttg aggacggcaa cacggtcgcc gagttcgagc gcttcttcct
     1321 gatcgtgcgt gatgaagatc gacggcacgt ttaccttctt cagcagcgcg cgtatctcgc
     1381 ggcgcaggtg cacgcgcgtt tgcgtgtcga gcgcgccaaa cggctcgtca aacaggagca
     1441 cttcgggttt gtaagcgagc gtgcgcgcga tcgcgacgcg ctgctgctgc cctcccgaaa
     1501 gttgcgacgg atactttttg cggtgctctt cgagttggac cagctgaagc agctcgttca
     1561 cagtctcgcg aatctctttg cgctttcgtt tgcggatccg caagccataa ccgatgttct
     1621 tttcaaccgt catcttcggg aacaacgcgt acgactgaaa gatgacgccg acgccgcgtt
     1681 cacgcgccgg gagttccgtc acttcgcgtc cgtgaagaat aactttgccc gtgtacggca
     1741 tttcgagccc ggcgatgatg cgcaggatgg tcgtcttgcc actgcccgat gcgcccagca
     1801 acacgagcac ctcaccttca gccacatcga agctgatgtt ttcaagcacg ctggctttgc
     1861 cgaagcgttt gctgattccg atcacacttg cagcagccgg cggcgtcggt gtgttcgaca
     1921 ccggttcgac aactctcgga atcaatgttt cctttgcctt aaccatcgct atgtctcagt
     1981 cgacgtcaag ccaatcgcgt gccagaaaca acacgctgaa aatcagaaat gaaaaaagcg
     2041 cgagcacaat cgcaatcgca tttgcggctt cgatctggcc ttcgttgaac ttcgcaaaga
     2101 tgtagagcgg cgcggtttgt gtgcgcccgg caagatttcc cgagacgagg atggtcgagc
     2161 caaactcgcc gaccgcgcgt gcaaacgtca ataatgcgcc ggagacaatc gcgccacgca
     2221 atgacggcaa ggtcacgtac cagaatgtct gccagcgggt ggcgccgatg gttgccgacg
     2281 cttcttcgag cgaacgactc atcgtgtcga aaaccggctt gattactccg aaagcgagcg
     2341 ggaaagtcac gaaaatattt gcgaggatga tcgcgcccgc agtgaacatg atctgaaaac
     2401 catgcggcgt cagcaaccgt ccgagtgtgc catatggacc ccaaagcagg agtaatgcga
     2461 agcccgcgac agcagtcgga atcgcggtcg gcagcgaaat cgtgacgata agtgcgttgc
     2521 ggccgaggaa gttgtaacgt gagagcacgt acgcggcgaa aaggccgaag aggacgttga
     2581 aaacagt
//
LOCUS       DASSFQ010000253         9794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1749581, whole genome shotgun sequence.
ACCESSION   DASSFQ010000253 DASSFQ010000000
VERSION     DASSFQ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749581"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFH96_09180"
     CDS             complement(<1..322)
                     /locus_tag="VFH96_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET6974183.1"
                     /translation="MSDLDDKLAQIKTALAELRASTAGGPATRKQLDALFRQVHNVKA
                     VAAADGLTDLSRAAHELENVLHSLRTGESTLDNHLLQQLGETLVESLPLPNEILSALK
                     TEEKH"
     gene            complement(329..817)
                     /gene="nusB"
                     /locus_tag="VFH96_09185"
     CDS             complement(329..817)
                     /gene="nusB"
                     /locus_tag="VFH96_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6974184.1"
                     /translation="MGSRRKARECALQMLFAADVAEMSADEVVRSYWAELGETDLDAT
                     AREFATRLAARTLSNLELLDERIRSRAEHWRISRMAVVDRNILRLAVYEFLYEPTPRT
                     VAINEALEIARRFSTYEATQFINGILDAIKRDLDEQLPQKNVQPEAEDSEPESSEPAE
                     DE"
     gene            complement(923..1462)
                     /gene="ribH"
                     /locus_tag="VFH96_09190"
     CDS             complement(923..1462)
                     /gene="ribH"
                     /locus_tag="VFH96_09190"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET6974185.1"
                     /translation="MRLEASPILRNVVTIQLAESNRLQPTVYEGRLNAEAFRFAIVAS
                     RWNELISARLVEGALDSLKRLGAKDANVSVYRVPGAFEIPLLALRLAQSRKFDAVICL
                     GTIIRGQTPHFDYIASEVARGISHAGLTSGLPVVFGIVTADTVDQALDRAGVKLGNKG
                     FEAAMTAVELVNLYKEAFE"
     gene            complement(1462..1854)
                     /locus_tag="VFH96_09195"
     CDS             complement(1462..1854)
                     /locus_tag="VFH96_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974186.1"
                     /translation="MSEDFTQKLPKNGNEEILTAITKIDNRLQSLEQKVDGLENKVVG
                     LEKKVDERLYDTRPIWHRVVADIAQLQAGQERLEKGQLAMSADLVRVSRDQIVINDSI
                     RKIQLDYHTIDDRLRRLELNRNPSNSST"
     gene            complement(1958..2851)
                     /locus_tag="VFH96_09200"
     CDS             complement(1958..2851)
                     /locus_tag="VFH96_09200"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET6974187.1"
                     /translation="MEIRKVGVLGCGLMGSGIAQVSASAGFETVVKEVSEDLIAKGFG
                     GIEKSLAKFAEKGTITSDQQKEIRGRLSGTTSFNDLAACDIIIEAIIENLDEKRSTYK
                     QLDEICKPETIFASNTSSLSITEMMTATSAERQRRFIGMHFFNPVPLMKLVEVVKTIL
                     TDADVYEEAVEFGKKLGKVPVRAGDKTGFIVNRLLVPYMLDAIRALEEGVGSIVDIDN
                     AMKLGCGYPMGPFTLGDFVGLDTTYYIAEIMFNEFREKRFAPPPLLKRMVLAGLYGRK
                     SGRGFYDYTRDAKNPTPMNLV"
     gene            complement(2875..4149)
                     /locus_tag="VFH96_09205"
     CDS             complement(2875..4149)
                     /locus_tag="VFH96_09205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6974188.1"
                     /translation="MCRSCGAIVGAGETQCAVCGASTSTQPTAHAGPRPPDRETIRFA
                     RAVLNRPYKFTIAFFVANLFVFLLMWESSGLTFSALQQVFPEAVLVTYGAKLNYLINP
                     PNNQWWRFITPMFVHIDILHLVMNMFSLLILGPFVEKLYGSAKFVVFYVVTGIAGTVG
                     SYLAVRPSLARGLLGSFIFHGVDAPAAGASGALFGLVGVLFVFGIKYRRELPEGFRRV
                     FGTGLLPIIFINLFIGFLGRSVVSNAAHLAGLFVGALLALFIDYRRPGARLSVTTLWR
                     ALQVVCLIVIALGAYKVVRNFNRPIPGIVRATPNANALIFLNYVNAMNQVQEKVSAVI
                     HTNDLSNLEQVTQSAMQAPVPDARAAELRFRLLDILSRLAAAAAEASPPPDNAPRRPP
                     VLDQKLVDEYTNWRKEYDEWLKVAAKTYTGMP"
     gene            complement(4329..4865)
                     /locus_tag="VFH96_09210"
     CDS             complement(4329..4865)
                     /locus_tag="VFH96_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET6974189.1"
                     /translation="MADQPFLTDIKTLRERARQHIENGAVTSGYSADRETVIKLLNEA
                     LATEIVCVLRYRRHYFMASGINAESVATEFLQHANEEQGHADQIAHRIVQLQGEPDFN
                     PDGLLTRSHAEYVEGTTLVDMIKEDLVAERIAIDSYREMITYFGKQDPTSRRMMEEIL
                     AVEEEHADDLVNMLQKMG"
     gene            complement(5011..6930)
                     /locus_tag="VFH96_09215"
     CDS             complement(5011..6930)
                     /locus_tag="VFH96_09215"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HET6974190.1"
                     /translation="MSAQERPKVRAPEITGGQGWLNTDKPLSLAALKGKIVLLDFWTY
                     GCINCIHIIPDLKKLETKYANQLVVIGIHSAKFQNEKETENIRRIILRYEIEHPVYND
                     SEYAVWQSYGVRAWPTQVLIDPAGYIVGGVSGEGNYDVIDQTVAKLITDFRSKGELNE
                     EPLKLVLERAKVGELPLAFPGKILADAKTDRLFIADSNHNRIVVTKLDGTLVDTIGTG
                     DAGAADGAFDKATFYRPQGMALAGDNLYVADTENHLIRRVDLKSRNVETIAGTGQQSR
                     EYFKTGPARSVALSSPWDLQLVDRVLYIAMAGPHQIWMLDLNKNEVSTFAGSGREARI
                     DGPVRDAGFAQPSGMATDGKKLYVADAESNIIREIDLAGRTVKTLVGGDLFEFGDVDG
                     KGDDVRLQHPLGLLTYGDKLLIADTYNHKIKELDPKAEKVTSLLGTGKPGQSDGSAPS
                     FYEPAGLALANGNLYVADTNNHAIRVVDLKTKRTSTLKLNGLTPPMKNVQALETASGP
                     NAEEIKVAAQKFRAGAEGALQIDVELPEGYHLNPLAPQRYKVSVDGKNLAVDEKTAAR
                     SSKDLKLPVRVPLNATANGPATVRAQVTLFYCREDNTGTCRIKTLVWQVPVEVTSDAN
                     APTELKVRGKLSVEP"
     gene            7064..9514
                     /locus_tag="VFH96_09220"
     CDS             7064..9514
                     /locus_tag="VFH96_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET6974191.1"
                     /translation="MNKQAFTTLEYQHLLELIKRNAQTEAGQRRVETLSPIDDASELR
                     HALAALAECVRLRARGVKWYFNGLSEPSETIARLRVEGASLDPLAILQMARLCDQAMS
                     ARAAILAERDEAPVLWQLVQDLPRDLNTLIARVTNKILPSGEIDDRASPELAGIRHEI
                     NVLRSRITRSLEGLMRRSAEAIQDELVTIRNDRFVIPVKADHRGRVQGVAHGYSSSGA
                     TAFIEPLETIDANNELQNLHESEAREIAKILFGLTEELRAQLPGIEMAAEAVAELDFI
                     NSRAVFHQSFDCVIPEIQTETNGSGELELIEARHPLLEENLRATGSAVVPVSFSLNDE
                     KNAMVISGANAGGKTVVLKTTGLLALMAISGISVPAREARFPFYATVLADIGDHQSLA
                     ANLSTFTSHAANIARMIELCEAPALVLLDEVGTGTDPEEGSALGVAVVDHFRRACSAH
                     VIATTHYSGLKMYAANQEGVLNASVEFDEKTLRPTYRLLVGVAGASSGLEIARRFGVP
                     TQIVENAMRSVADSSLQASEYLRRIKRESEDAEALRIALEEERQAVAEKFASLDKEAA
                     KQERERQSMFDQTVQRVIAGLEKHARELVAKIEDRTERVRVERDAQRQIAEIKRTAQR
                     EVRVVREGQTVQPERVEPEAPVADAPEYVAAAPREIVVGDRVKLRSFGSVGIVDQIKN
                     GVAEVRVKSLRLREKLENLDLVEATTPKPQAGKLEKLRRSAETESRLAPADDKAQSEL
                     NVIGQTTDEAVDAVDKFLDEASLASLSKVRIIHGHGTGALRRAIGALLDGHPHVSRFL
                     PAPPDQGGAGATLVELRQ"
BASE COUNT         2167 a         2683 c         2676 g         2268 t
ORIGIN      
        1 cgtgtttttc ttctgtcttg agggcactca atatttcgtt tggaagaggg agactttcta
       61 cgagggtctc tccgagttgt tgtagcaggt gattgtcgag tgttgactcg ccggtgcgta
      121 aagaatgcag cacgttttcg agctcgtgag cggcgcggct gagatcagtg agaccatcgg
      181 cggcggcgac tgccttgacg ttgtgtacct ggcgaaagag tgcgtcgagc tgtttgcgtg
      241 ttgcggggcc gccggcggtc gaagcgcgta gttctgcgag ggcggtcttt atttgtgcga
      301 gcttgtcatc gaggtccgac aagtttttct actcatcttc agcgggctca cttgattcgg
      361 gctcagagtc ttccgcctcg ggttgaacat ttttttgcgg cagctgttcg tcgagatcgc
      421 gttttatggc gtcgagaatg ccgttgatga attgcgttgc ttcgtacgtc gagaaccggc
      481 gcgcgatttc gagtgcttcg ttgatggcga cggtgcgtgg cgtcggttcg tacagaaatt
      541 cgtagacagc gagacgcaga atattgcgat cgacgaccgc catgcgtgag atgcgccagt
      601 gttcggcgcg cgagcggatg cgctcgtcga gtagctccag atttgagagc gtacgcgcgg
      661 cgaggcgcgt ggcaaattcc cgcgctgtgg cgtcgaggtc agtttctcca agctcagccc
      721 agtagcttcg gacgacctcg tccgcagaca tttcggcaac atcagccgca aagagcattt
      781 gcaaggcgca ttcacgcgcc ttacgtcgcg aacccataaa gtattaccaa aaactgttta
      841 agccacaaaa gttatccaca gattacacag attctggggt tgctctgtgt aatctgcgta
      901 atctgtggat aactttgccg gctcattcaa aagcttcctt ataaagattt accaactcca
      961 ccgccgtcat ggcggcttca aaccctttgt tcccgagttt cacacccgca cgatcgagcg
     1021 cctgatcaac tgtatccgct gtaacgattc cgaatacgac cggcaatccg ctcgtcagac
     1081 ctgcatgtga aatgccacgc gcgacttcgc tcgcgatgta atcgaaatgc ggcgtttgac
     1141 cgcgaatgat cgtgccgaga cagattaccg cgtcaaactt tcgcgactgt gcgagtcgca
     1201 acgccagcag cggaatctca aaagcgcccg gcacccgata gacgctgacg ttcgcgtcct
     1261 tcgcgccgag tcgcttcaaa ctatcgagcg cgccttcgac gaggcgagcc gagatcaact
     1321 cgttccagcg actcgccacg atagcgaagc gaaacgcttc agcgttgaga cgtccttcgt
     1381 acaccgttgg ctgcaaccga ttcgactccg cgagctgaat agttactacg tttcgcagta
     1441 taggagatgc ttctaagcgc atcaagttga tgaattcgac gggttacgat ttagttcaag
     1501 acgacggaga cgatcatcaa ttgtgtgata gtcgagttga atcttgcgga tagagtcatt
     1561 gatgactatc tggtcacgac taacccgcac taagtctgcg ctcatggcta attgaccctt
     1621 ttccaatcgt tcctgcccag cctgtaattg ggcaatatcg gccaccactc tatgccagat
     1681 tggacgagta tcgtagagtc tttcgtcaac ctttttctca agaccgacaa ccttgttctc
     1741 aaggccatcg actttttgtt cgaggctctg aagtcgattg tcaatctttg tgattgctgt
     1801 gagaatttct tcgttcccgt tctttggtag tttctgtgtg aagtcttcgc tcattcattt
     1861 ctcccttcag ggagtatcct aacaccttga tcctcatcgc gatagacgcg aaaacggcta
     1921 tttgttgtcg aaaacggcta ttctcacgcc gacgaaccta aacgaggttc atcggcgtgg
     1981 ggtttttggc gtccctggtg tagtcgtaga agccgcggcc ggattttcgt ccgtacaatc
     2041 ccgccagcac catgcgcttc aacaatggtg gcggcgcgaa acgcttctca cgaaactcat
     2101 tgaacatgat ctccgcgatg taatacgtcg tatccagccc aacgaaatcg ccgagcgtaa
     2161 acggacccat cggataacca cacccgagct tcatcgcgtt atcaatatca acaatcgacc
     2221 ccactccctc ttccagcgcg cgaatcgcgt cgagcatgta cggcaccagc agacgattca
     2281 cgatgaaacc cgtcttatca ccagcacgca ccggcacctt tccgagcttc ttcccaaatt
     2341 ccaccgcctc ctcgtacacg tccgcatccg tcaatatcgt cttcactacc tcaaccagct
     2401 tcatcaacgg cactggattg aaaaaatgca tcccaataaa acgacgctga cgctccgcgg
     2461 acgtcgccgt catcatctcc gtgatcgaca gcgacgacgt attcgacgca aagatcgtct
     2521 cgggcttgca gatctcgtcc aactgtttat acgtgctgcg cttctcgtcg agattctcta
     2581 tgatcgcttc gataatgatg tcgcaggctg ccaggtcgtt gaatgaagtc gtccctgaca
     2641 aacgaccgcg aatctccttt tgctgatcgg acgtgatcgt tcctttttca gcaaacttcg
     2701 ccagcgactt ctcgataccg ccaaaacctt tcgcaatcag atcctcagac acttccttca
     2761 ccacagtctc aaatcccgca ctggccgaca cctgcgcgat gcctgatccc atcagtccgc
     2821 atcctaaaac tccgactttc ctgatctcca tttcgctctc ctataaaagt ttgatcacgg
     2881 cattcccgtg taagtttttg cagcgacttt caaccactca tcatattcct tacgccaatt
     2941 tgtatactcg tcaaccagct tttggtccaa caccggcggt cgcctgggcg cgttgtcagg
     3001 cggcgggctt gcctcggccg cggctgctgc cagtctcgaa agaatatcca gcaaccggaa
     3061 tctcaattcg gccgcacgcg cgtcgggtac cggcgcctgc attgcacttt gagtcacctg
     3121 ctcgagatta gacaagtcgt tagtatgaat cacggctgaa actttttcct gaacctggtt
     3181 catcgcattc acatagttca gaaaaatcag cgcgttcgca ttcggcgtgg cacgcacaat
     3241 tcccggtatc ggtcgattga aatttcttac gactttgtac gcccccaacg cgatcacgat
     3301 caagcacacg acctgcaacg ctcgccacaa cgttgtgaca ctcaaacgtg ctccgggccg
     3361 gcgataatcg ataaacaacg ccagcaaagc gcccacgaag agtcccgcca ggtgcgcggc
     3421 attgctgaca acgctgcgcc ctaaaaaacc aatgaacaaa tttataaaga tgatgggcaa
     3481 caggcccgtt ccaaacacgc gcctgaatcc ctcgggaagc tcgcgccggt acttaatgcc
     3541 aaacacaaac agtacgccga caagtccaaa cagcgctccg gaagccccag ccgccggcgc
     3601 gtcgacgcca tggaaaatga agctgcctaa cagtccgcga gcgagcgacg gtcgcaccgc
     3661 gagataactc cccactgtgc cagcaatacc cgtcaccacg tagaacacaa caaacttcgc
     3721 cgaaccgtaa agcttctcga caaatggtcc aagtatcaac aaactgaaca tgttcatcac
     3781 gaggtgcagg atgtcgatgt gaacaaacat tggcgtgatg aagcgccacc actgattatt
     3841 cggcggattg atcaggtaat tcagcttcgc gccgtacgtg acgaggaccg cttccggaaa
     3901 gacttgttgc agcgcgctga acgtgagccc gctcgactcc cacatcagca gaaatacaaa
     3961 caagttggcg acgaagaacg cgatcgtaaa cttgtacggt cgattcagaa cggcgcgagc
     4021 gaagcggatc gtttcccggt ccggtggacg tggaccagcg tgagcggtcg gttgggttga
     4081 ggttgaggca ccgcagacgg cgcattgagt ttcgccggcg ccgacgattg ccccacaact
     4141 acggcacatt gttggccggc cggggttagt ttgattggat tgactttcgg acaacgagca
     4201 gaagactaac aagcgtgatg ctccgtgcgc aaagcatcac gctggaagtc gtttcacagg
     4261 attaacaaga tctgacatga tttacatgtt aatcctgaaa aatcttggaa atccctgtct
     4321 acaactaact agcccatctt ctgaagcatg ttcaccaggt cgtcggcgtg ttcctcttcg
     4381 acggcgagaa tctcttccat catccttctc gacgtcggat cctgtttgcc gaagtaggta
     4441 atcatctcgc gataactgtc gatcgcgata cgctcggcca cgagatcctc tttgatcatg
     4501 tcgacgagcg tcgttccttc gacatactcc gcgtgactac gcgtcagaag tccatcgggg
     4561 ttgaagtccg gctcgccttg taactgcacg atccgatgcg caatctggtc ggcgtgtccc
     4621 tgttcttcgt ttgcgtgctg cagaaactcc gtagccacgc tttccgcgtt gatgccggaa
     4681 gccatgaaat aatggcgccg gtagcggagc acacaaacga tctcggttgc gagcgcttcg
     4741 ttcaaaagtt tgattacggt ttcgcgatcc gcgctataac ctgacgtgac agcaccattc
     4801 tcgatgtgtt gtcgggcgcg ttcgcgcaaa gttttgatat cagtgagaaa tggttgatca
     4861 gccacgagct cactcctctg caatatgaat ttaggggagc ccggatctta acacagatca
     4921 gatcggtttc aatctgcagc gaagtacaca aaaggcacaa aaagacagaa ggcacatgaa
     4981 tacttttctg atttttgtgc cttttgtgta ctaaggttct accgaaagct tgccacgtac
     5041 tttcaactct gttggcgcat tcgcatcact cgtcacctcg actggaactt gccacacgag
     5101 cgttttgatt cgacaggtgc ccgtgttgtc ttcacgacag taaaacaggg tgacctgcgc
     5161 gcgaacggtc gccgggccgt tagcagtcgc gtttagcggc acacgcaccg gaagcttcag
     5221 atctttactg gagcgggccg cagtcttttc atcgaccgcc aaattctttc cgtcaacaga
     5281 aactttgtaa cgctgcggcg cgagcggatt caggtgatag ccttcaggca actccacatc
     5341 gatctgaagc gcgccctcag cacccgcccg aaacttctgc gccgcgacct tgatctcttc
     5401 agcatttgga ccagacgccg tttccagcgc ctgcacgttt ttcatcggcg gcgtgagacc
     5461 gttgagcttc aacgtactcg tgcgttttgt tttcagatcg acgacgcgaa tcgcatgatt
     5521 gtttgtgtca gcgacgtaga gatttccatt cgcgagggcc aatccggcgg gctcgtaaaa
     5581 cgaaggcgct gagccatctg actgtcccgg ctttccggtg ccgagcaggc tcgtgacttt
     5641 ctcggctttt ggatcgagct ctttgatctt gtgattgtaa gtgtcggcga tcaggagttt
     5701 gtcgccatag gttaagagac cgagcggatg ttgcagtcga acatcgtcac ccttaccgtc
     5761 aacatcgcca aactcgaaca gatcgccgcc aacgagagtc ttcactgtac gtccggcaag
     5821 atcgatctcg cggataatgt tcgactcggc gtcagcgacg taaagcttct tgccgtcagt
     5881 cgccataccg gaaggttggg cgaaacccgc gtcgcgaacg ggaccgtcga tgcgtgcttc
     5941 gcgacccgat ccggcgaaag tcgagacctc gttcttgttg agatcgagca tccagatctg
     6001 gtgtggacca gccatcgcga tgtagagcac acgatcgacc agttgcaaat cccacggcga
     6061 gctgagtgcg accgatcgcg ccgggcccgt tttgaaatac tcgcgcgatt gctgccctgt
     6121 accggcgatc gtttcgacgt tgcgtgattt cagatcgacg cgacggatca gatggttttc
     6181 agtgtcggcg acgtaaagat tatcgccggc gagagccatg ccctgcggac ggtagaaagt
     6241 tgctttgtcg aaggcgccgt cagcagcgcc ggcgtcgccc gtaccgatcg tgtcgacgag
     6301 cgtgccgtcg agttttgtga ccacgatgcg attgtgattc gagtcagcga tgaacagtcg
     6361 atcggtcttc gcgtccgcaa gaatcttgcc gggaaaggcg agtggcagct cgccgacttt
     6421 ggctcgttcg agaacgagtt tcaaaggctc ttcgttgagc tcgcctttgc tgcgaaagtc
     6481 tgtgatgagc ttcgcgaccg tttgatcgat gacgtcgtaa ttgccttcgc cggaaacacc
     6541 gccgacgatg taaccggcag gatcgatcag cacctgcgtg ggccatgcgc gcacgccgta
     6601 actttgccag accgcgtatt ctgaatcgtt gtaaaccgga tgctcgatct cgtatcgcag
     6661 gatgatgcgc ctgatgttct cagtctcttt ttcgttctga aacttagcgg agtgaatgcc
     6721 gatcaccacg agctggttcg cgtatttcgt ctcgagcttt ttcagatcgg gaatgatgtg
     6781 gatacagttg atgcagccgt aggtccagaa atcgagtaga acgattttgc ctttcaaagc
     6841 ggctagcgag agtggcttat cggtgttgag ccaaccctgg ccgccggtga tttccggagc
     6901 gcgaactttt ggtctctcct gtgcggacat aggtacaccc ttagctatca aaatcagaac
     6961 gaaaattgcg accacgaatt tgacggattt catatggacg accattgtac gatccgtctg
     7021 catccgtgca aatccgtggc taatatttta acctttacgc ctcgtgaaca agcaggcatt
     7081 tacaactctc gaataccagc atctgctgga gctgattaaa cgaaacgcgc agaccgaagc
     7141 cgggcagcgt cgcgttgaaa ccctgtcgcc gattgacgac gcgtctgagc tgcgccatgc
     7201 gctcgccgcg ctcgccgaat gcgtgcggct gcgcgcgcgc ggcgtcaaat ggtatttcaa
     7261 cggattgtcc gagccgtctg agacaatcgc gcgattgcgt gttgaaggcg cgagtctgga
     7321 tccactcgca atcttgcaaa tggcgcgtct ttgcgatcag gcgatgtcgg cgcgggccgc
     7381 gattcttgcc gagcgcgatg aagcgccagt tctttggcaa ctggtccagg atctgccgcg
     7441 cgatctcaac acgctcattg cgcgcgtcac gaataaaatt ttgccgagtg gagagatcga
     7501 cgatcgcgcg agcccggagc tggcgggcat tcgtcacgag atcaatgtgc tccgttcgcg
     7561 gatcacgcgc tcgcttgaag gactgatgcg gcgttcggct gaggcgattc aggatgagct
     7621 cgtgacgatt cgcaacgatc gtttcgtcat tccggtgaaa gccgatcatc gcgggcgtgt
     7681 tcagggtgta gcgcacggtt attcgtcatc cggtgcgacg gcttttatcg agccgctcga
     7741 gacgatcgat gcgaataatg agttacagaa cctgcacgag agcgaggcgc gcgagatcgc
     7801 gaagattctt ttcggtttga cagaagaatt gcgtgcgcaa ctgcccggga tcgagatggc
     7861 ggcggaggct gtcgccgaac tcgatttcat caactcgcgt gcggtttttc atcagagctt
     7921 cgattgcgtc attcccgaaa ttcaaacgga aacaaacggc agcggtgagc tcgagctgat
     7981 cgaagctcga catccactgc tcgaggaaaa cctgcgcgcg acgggtagcg cggttgtgcc
     8041 agtctcgttt tcactcaacg acgaaaagaa tgcgatggtg atctccggcg cgaacgcggg
     8101 cggcaagact gtggtactga agacgacggg tttgcttgcg ttgatggcga tctcggggat
     8161 ttcggtgccg gcacgtgagg cacgttttcc attttatgca acagtgctcg cggacatcgg
     8221 cgatcatcaa tcgctggctg caaatctctc gacgtttacg tcacacgcgg cgaacatcgc
     8281 gcggatgatc gagttgtgtg aagcgccggc gctagtgctg ctcgatgaag tcggaactgg
     8341 aactgatccc gaagaggggt cggcgctcgg tgtcgctgtc gtcgatcatt tcagacgcgc
     8401 ttgcagtgcg catgtcatag cgacgacgca ttacagcggg ttgaagatgt atgcagccaa
     8461 ccaagagggt gtgctgaacg cgagtgttga gtttgatgaa aagacgctgc gcccgactta
     8521 ccgcttgctc gtaggcgtcg ctggtgcgtc ttctggattg gagattgcgc ggcggtttgg
     8581 cgttccgacg caaatcgtgg aaaacgcgat gcgatcggtg gcagactcgt cgttgcaggc
     8641 gtcggagtat ttgcgacgaa tcaagcgcga gtctgaagac gctgaagcat tgcgcattgc
     8701 gttggaagag gaacgacagg ccgtcgctga gaagtttgcg tcgctcgaca aggaagccgc
     8761 gaaacaggaa cgcgaacggc aatcaatgtt tgatcaaacg gtgcagcggg tgattgccgg
     8821 gctcgagaaa catgcgcgcg agctggttgc gaagattgaa gatcgtacgg agcgtgttcg
     8881 tgttgagcgt gatgcgcaac ggcagatcgc ggagataaag cgcacggctc agcgtgaagt
     8941 tcgggtcgtg cgtgaagggc agactgttca accagaacgc gttgaaccgg aagcgccggt
     9001 tgcagacgcg ccagaatacg tcgcggctgc accgcgcgag atcgtggttg gcgatcgcgt
     9061 taagcttcgc tcgttcggat cggtcggtat tgtggaccag atcaagaatg gtgttgctga
     9121 agtgagagtg aagtctctca ggctccgcga gaagctcgag aatcttgatt tagtcgaagc
     9181 cacgactcca aaaccacaag caggcaaact cgaaaaactg cgacgctccg ccgaaaccga
     9241 gagtcgtctc gcgcctgctg acgacaaagc gcaatcggag ttgaacgtca ttggacagac
     9301 gacggacgaa gcagttgacg ccgttgacaa gtttctcgac gaagcttctt tagcaagtct
     9361 gagcaaagtc agaatcattc acggtcacgg caccggcgcc ctccgccgcg caatcggcgc
     9421 cctgcttgac ggccacccgc acgtttcacg tttcctgccc gccccacccg accagggcgg
     9481 tgctggtgca acactcgttg agttgagaca atgacaactg atgactgaca ctgcatacaa
     9541 ccgattgcta aaacgtgtag tttcggatcc aaaggtgctc ggtggagagc cgcgtattcg
     9601 cggaacacgg attcatgtgg ctgtagtttt agactctttg gctgaaggcc ttacgccggc
     9661 tcaaatcgta gatcattttc ctccgcttac tgaagatgat gtgcgagcag cagtggcgta
     9721 tgcggcggag ttggcgcgta cagtttcagc tgcatgagga tcaagctgga ccactttacg
     9781 cttgacacgg attt
//
LOCUS       DASSFQ010000254         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2063624, whole genome shotgun sequence.
ACCESSION   DASSFQ010000254 DASSFQ010000000
VERSION     DASSFQ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063624"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VFH96_09225"
     CDS             complement(<1..1334)
                     /locus_tag="VFH96_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HET6974192.1"
                     /translation="MWKFKNPIKHPILETVWPAAEDAARSRLFSPVAIGKLEARSRTW
                     VPAMVPWRATEDGFVTQQNLDWYQRFAEGRPGVLVVEATGVRDIPSGPLLRIGDDRFI
                     PGLRKLVDVVREASDGQTLLFIQIIDFLAVKRRPEKSKYFQRFLVVDEHHRRALAEVT
                     EHTDWFTESDTDVRDYLENASDEIVDFVLRPRELEALRFGYRERVTDTHLQHIRDLPL
                     ELPAIFAAAAERAREAGFDGVELHYAHAYTMAGFLSAQNDRADGYGGSREHRVRLPLE
                     VYREVRNRVGDDYVIGVRFLADEVIEGGSRVEDAIYYAKEFAGASFDFLSLSKGGKFE
                     DASEPKVGQAVYPYTGQSGYECMPTVLSDATGPFGRSVPLVAAIKKAVNDAGFAPPIV
                     ATGGISTFDHAEEILRLGHADVIGMARQALADPDWFLKVKLGHGSEVRRCTYT"
     gene            1411..2598
                     /locus_tag="VFH96_09230"
     CDS             1411..2598
                     /locus_tag="VFH96_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6974193.1"
                     /translation="MTDDLLKWRNEFPILDKAVYLISHSLGAMPRATYDRLQEYADIW
                     ASRGVRAWAEGWWDMPVTVGDEVARIIGADRGTVVMHQNVSICQSLVLSCLKPTPQRN
                     KIVYSELNFPSVMYVYEAHARDGSLRIDIVKSDDGMTVPLERMLAAIDETTLLVPFSH
                     VLFKSAFLQDAKAIIDRAHEVGAMVVLDTYQSAGTVPFSVKELNADFATGGSVKWLCG
                     GPGAGYLYVRPDLQTTLEPKTTGWMAHESPFAFDTSLRYAPNITRFLHGSPAIPALYA
                     AQSGYRIINEIGVEKIREKSMRQTEYLINLAEEAGFKVTSPKNVSQRGGTITIAHEHA
                     GAVTKELIHREFIVDYRPGAGIRISPHFYTTDDELGLVIKEMKKISESESYREHEAAG
                     AAF"
     gene            2599..3453
                     /locus_tag="VFH96_09235"
     CDS             2599..3453
                     /locus_tag="VFH96_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET6974194.1"
                     /translation="MPNNYGDNPPLSYNKYLRVQDLINLQDCLSTPAHHDELLFITVH
                     QAYELWFKQILHELDAAVELLENDHVSAATRAIKRVVEIEKLLVNQIHILESMTPISF
                     LAFRDQLNPASGFQSMQFREIEFSSGLKDKGILREFATDQFAHERLAERMSRPTLAEC
                     FYRVLQRRGLQAPLSTSDADQTQQKKDYEARTRAVLEVLTHFEDRYEEFQLAEALLEH
                     DEYFALWRSHHIKMVERMVGAKRGTGGSEGIGYLKTTLDKKFFPELWEARTYLDVGHG
                     DGGCPFAH"
BASE COUNT          843 a          995 c          913 g          761 t
ORIGIN      
        1 gtgtaagtac aacgccgcac ctctgagcca tgaccaagtt tgaccttaag aaaccaatcc
       61 ggatcggcaa gcgcctggcg agccatcccg atcacatccg catgtcccaa gcgcaagatc
      121 tcttcggcgt gatcgaacgt cgagattcct ccggtcgcaa cgatcggcgg cgcaaatcca
      181 gcatcgttga ccgccttctt aattgcggca acgagcggga cgcttcgtcc aaatggtcca
      241 gtcgcatccg aaagcacagt cggcatgcac tcatagccgc tttgacccgt gtaaggataa
      301 acagcctggc ccactttcgg ctccgacgcg tcttcaaact tcccgccttt cgaaagcgag
      361 agaaaatcaa aactcgcgcc tgcaaactcc ttcgcgtaat agatcgcatc ttcaacacgg
      421 ctgccgcctt caataacttc atcagccaga aagcgcacac cgataacgta atcgtcgccc
      481 acgcgatttc gcacttcacg atagacttcg agcggcagac gcacgcgatg ttcgcgcgat
      541 ccgccgtaac cgtcggcgcg atcgttctgc gcgctcaaaa aacccgccat cgtgtaagcg
      601 tgtgcgtagt gaagctcaac gccatcgaaa ccagcctcgc gggctcgctc cgcggctgcg
      661 gcaaagatag cgggcagctc cagtggtaaa tcgcgaatgt gttgcaggtg cgtgtcggta
      721 acacgctctc gatatccgaa gcgtagcgct tcgagttcgc gtggacggag cacgaaatca
      781 acaatctcgt cgctggcatt ctccagatag tcgcgcacgt ctgtatccga ctcggtaaac
      841 caatccgtat gctcggtgac ttcagccaac gcgcgacgat gatgctcgtc tactaccaaa
      901 aatcgttgaa aatatttcga cttctcagga cgtctcttga ccgcaagaaa gtcgatgatc
      961 tgaataaaca gcaacgtctg tccgtcgctc gcctcacgca caacatcgac aagtttgcgc
     1021 agacccggaa tgaatcgatc atcgccgatg cgcagtaaag gaccgctcgg gatgtcgcgc
     1081 acgccggtgg cttcaacgac gagcactccc ggccgccctt cggcaaagcg ttgataccag
     1141 tcgagatttt gctgcgtgac aaaaccatct tcagtcgcgc gccacggcac catcgccgga
     1201 acccacgtcc ggctgcgcgc ttcaagcttg ccaatggcca caggactgaa cagccgagag
     1261 cgagccgcgt cttccgcggc cggccagaca gtctcaagta tcggatgttt tattggattc
     1321 ttgaatttcc acatttcatc tcgccacaga ttacacggat tacacggata tgccgaatct
     1381 gttaaattaa cccaagcagg aggacgatgc ttgactgacg atttactaaa gtggcgtaac
     1441 gagtttccca ttctcgacaa agctgtttat ttgatctcgc acagccttgg ggcaatgccc
     1501 cgggcaactt acgatcgact tcaggagtac gcggacatct gggcgagccg cggtgtgcgt
     1561 gcgtgggcgg aaggttggtg ggacatgccc gtcaccgttg gtgacgaagt cgcgcgcatc
     1621 attggcgccg acaggggcac ggtcgtgatg catcagaacg tctccatctg ccagtcgctc
     1681 gtgctctcgt gtctgaaacc gacgccacaa cgaaacaaga ttgtctattc agagttgaac
     1741 tttccatcgg tgatgtacgt ctacgaggct catgcgcgtg acggaagtct acgcatcgac
     1801 atcgttaaat cagacgacgg catgacagtt ccactcgaac gaatgcttgc tgcgatcgac
     1861 gaaacaactc tcctcgttcc gttctcgcat gtgcttttca agagcgcgtt tctccaggac
     1921 gcaaaagcta tcatcgacag agcacatgaa gtcggcgcga tggtcgtgct cgatacttat
     1981 caatcggcgg gtacagtgcc gttcagcgtg aaagagttga acgcggattt tgccaccggt
     2041 ggctcggtta aatggctttg tggtggacca ggcgcgggtt atctgtatgt gcggccggac
     2101 ctgcaaacta cgttagagcc aaaaacaacg ggctggatgg cccacgaatc accttttgcc
     2161 ttcgacacat cactccgcta cgcgccgaac atcaccaggt tcctgcatgg ctcgcctgcg
     2221 attccggctc tttacgcagc ccaatcgggt tatcgcatca tcaatgaaat cggagttgag
     2281 aaaattcgcg agaagagcat gcgtcaaaca gagtacttga tcaatctcgc cgaagaagca
     2341 ggcttcaaag ttacgagtcc caaaaatgtc agccagcgtg gaggtacgat cacgatagct
     2401 catgagcacg ccggcgcggt gacaaaggaa ctaattcacc gtgaatttat tgtcgattac
     2461 cggccgggcg caggcattcg catctcacca cacttctaca cgactgacga cgagctgggg
     2521 ctcgttatca aagagatgaa gaagatcagc gagagtgaga gttatcgcga acatgaagca
     2581 gcgggggcag cgttctaaat gcccaacaac tacggcgaca atccgcctct ttcttacaac
     2641 aagtatctcc gtgttcagga tctgattaat cttcaggact gtctttcaac gcccgctcat
     2701 cacgacgaac tcctattcat aaccgtccat caggcgtatg agctgtggtt caaacaaatt
     2761 ctccacgagc tcgatgcggc cgtagaatta ctcgagaacg atcatgtttc ggccgccacg
     2821 cgcgcgatca agcgcgtcgt cgagatagag aagttgctcg tgaaccagat tcacattctc
     2881 gagagcatga cgccgatcag ctttcttgcg tttcgcgatc agctaaatcc cgccagcggt
     2941 tttcagtcga tgcaatttcg cgagattgaa ttctcttcgg gtttgaaaga caaaggcatt
     3001 ttgcgtgagt tcgccactga tcagtttgcg cacgagcgac tggcagagcg tatgtcgcgt
     3061 ccaacgctgg ccgagtgttt ttatcgagtg ctccagcgtc gtggtttaca ggcgccgcta
     3121 agcacgtcag acgcggatca gacacaacaa aaaaaggact atgaagctcg cacgcgagcc
     3181 gtactcgagg ttttgactca cttcgaagat cgatacgaag agtttcaact tgccgaagca
     3241 ttgctcgaac acgacgaata cttcgcgctc tggcggtcgc atcacatcaa gatggtcgaa
     3301 cgcatggtcg gcgcaaagcg tggcaccggc ggatcggaag gcatcggtta cttaaaaacg
     3361 acgctcgaca aaaaattctt tcccgagctc tgggaagcac gcacttatct cgacgtcggc
     3421 cacggcgacg gtggttgtcc gtttgcacac tgatctctct tttggagtgc ggcggcctga
     3481 cgccgctttg ttatcgttaa agcccgatcg ag
//
LOCUS       DASSFQ010000255        10758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_426698, whole genome shotgun sequence.
ACCESSION   DASSFQ010000255 DASSFQ010000000
VERSION     DASSFQ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426698"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFH96_09240"
     CDS             <1..256
                     /locus_tag="VFH96_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974195.1"
                     /translation="IVFNAAVAVFAIGTLRLREATGEVLPKHEFSLRPFKRMTSWTAA
                     TTRRTTVRAKKARQRVRSRDVTSEFKKIDVSETPDKRAVS"
     gene            335..1834
                     /gene="hutH"
                     /locus_tag="VFH96_09245"
     CDS             335..1834
                     /gene="hutH"
                     /locus_tag="VFH96_09245"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET6974196.1"
                     /translation="MLELNGQQLTLQQIAAVAQGREQVVLAAEARARVEKARQVVQNI
                     VNEGRTVYGVNTGFGKLSDVSIEQSDLSQLQLNLVRSHSCGLGQPLSEAEARAMLLLR
                     ANVLAAGFSGARPLVIDTLCAMLENGVTPVIPEKGSVGASGDLAPLAHLALAAIGEGE
                     VFYEGERLSASEALKRANVAPLQLEVKEGLALLNGTQAMGAVGSLALNRAEKLVRLAD
                     VAGAMTLEALKGTPVAFDERIHAARPHPAQLAVAAHLRELLQESEIRKSHLNNDPRVQ
                     DAYSLRCMPQVHGAIRTALSHAIQVVEIESGSATDNPLVFSETGEVLSGGNFHGAPLA
                     LVFDYAAIALTDLMSITERRIDRLVNPDSNEGLPPFLTSQPGISSGFMMLQVTAVALL
                     NEAKVLAHPASIDNVPTDGGKEDHVSMGMTAATKLRGIVELAELATSIELLTAAQALE
                     YRSLAPGRGVKQAYDIVRKLVQPLTQDRSMAKDIERLRDAITAGEFAGC"
     gene            1956..2261
                     /locus_tag="VFH96_09250"
     CDS             1956..2261
                     /locus_tag="VFH96_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET6974197.1"
                     /translation="MANGMDDRRKSLEEEYFHRKNKEAIEKLRAKMKVAEEAKAAGLS
                     SMQCPRCDGTLKENKFEEVLIDICDKCGGVWLDSGELEQLTKKDTGGWFSRYWFSEG"
     gene            2258..3913
                     /gene="hutU"
                     /locus_tag="VFH96_09255"
     CDS             2258..3913
                     /gene="hutU"
                     /locus_tag="VFH96_09255"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET6974198.1"
                     /translation="MSHRVIHAPHGPQLSCKGWHQEAALRCLMNNLDPDVAERPDELI
                     VYGGAGKAARNWAAFDAIVRELQALENDETLLVQSGKPVAVFRTHEHAPRVLIANSNL
                     VGAWANWEHFHELERRGLMMYGQMTAGSWIYIGTQGIVQGTFETFAAMADRQFGGDLS
                     GKLVVSGGMGGMGGAQPLAATLNGAVFLGIDVDPERIERRVRTGYCDRIAMTLDEALD
                     ICEDARKQKRGVSVGLVGNCAEILPELVRRGVHVDAVTDQTSAHDPLNGYVPARMSLD
                     DAAELRASDPAAYVEKSMASMAQHVEAMLALKRAGAIAFDYGNNIRKQAFDAGCTDAF
                     EIPGFVPEYIRPLFCEGKGPFRWVALSGDPEDIRKTDDLALEMFPDDRTLNRWLRLAR
                     ERVHFQGLPARICWLGYGERAEMGEAINDLVKRGDLKAPIAIGRDHLDTGSVASPFRE
                     TENMKDGSDAVADWPILNALLNTASGASWVSFHHGGGVGIGYSLHAGQVSVADGTDEA
                     AKRLNRVLTNDPGLGVARHVDAGYEEAENTAREKSINIPLLAG"
     gene            3987..4865
                     /locus_tag="VFH96_09260"
     CDS             3987..4865
                     /locus_tag="VFH96_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET6974199.1"
                     /translation="MKTTTRLVRALLLIFCFATVVSARVDTVRFQSKLINATLPYNVI
                     LPTDYDTSKTTRYPVLYLLHGLTGHYSDWVSRTNVADYASEYRLIVVTPEGNNSWYID
                     SVGVANDKYESYVLNELIPDVQQRYRTIEARYGRSIAGLSMGGYGAFKFGLKTPATFV
                     FAGSMSGAFGVTRFTEKEIGPERFADSLKLFGALGSDTRAANDLYDIIGKLTPARVAS
                     LPYFYFDCGTEDAPLIFPFNRELAALMFEKKIPHEFRELPGDHSWGYWDRQVREILRI
                     ATDKMRMPSTKRISRA"
     gene            4858..5622
                     /locus_tag="VFH96_09265"
     CDS             4858..5622
                     /locus_tag="VFH96_09265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET6974200.1"
                     /translation="MLNFAIDVARDAGSLLVQRLGSAKVSTKGDINLVTEADIAAENL
                     IIDRIRSHYPQHGILAEESGEAVLVGGKRSEWKWIIDPLDGTTNYAHGYPCFCVSIAL
                     ERSGVLEIGVVYDPMRNEMFAAERGQGATLNDRQIRVSSVEELSGAMLCTGFPYNVRE
                     RPDFARGFTNFTMNAQAVRRDGSAALDLAYVACGRFDGFWEDGLSPWDVAAGAVLISE
                     ARGRITNFKDEPVNIYNEQILASNGLIHDAMMRVLS"
     gene            complement(5690..6367)
                     /locus_tag="VFH96_09270"
     CDS             complement(5690..6367)
                     /locus_tag="VFH96_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6974201.1"
                     /translation="MSNNGTARVKREDPRASPEISPLPQWALDSIRPVVGALSRVFFK
                     IEFNGLENVPKNGGLIIAANHQTYIDPFWLSVPIKRPTRYLAWSAAFRWPVVGRFLIW
                     FGAWPIALEGSDPAAIRSSLQFLRNGGAVVIFPEGARSTVSGDLDRFKVGAVRLAMEA
                     QVPILPVTIKGGNRVWPRGWRFPRPGKVVVTYHPLHHAEQCPNEETRACARRESEKLA
                     QVIASAL"
     gene            complement(6397..7119)
                     /locus_tag="VFH96_09275"
     CDS             complement(6397..7119)
                     /locus_tag="VFH96_09275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6974202.1"
                     /translation="MSEERNWKRIRERLALQLPVRVQVRETPDFEWTEITRLTNVTPF
                     GAGFTLKRPTEKGRLLFMTIPMPRQLRVFDHVEDQYRIWAIVRYMKASVGEKGPVFEI
                     GVAFIGKRPPASFETEPWKRYDISTTAFQALSAADEILIPLPSMDQRAYTRHNIAVDM
                     RVELVDIDGAVVASEYTVTEDISTKGATLFTTLEIPKGRFIRLTSEQHGITAHAAIRS
                     RSTGADGIPRIHVEFIDKEWPL"
     gene            7366..9927
                     /gene="gyrA"
                     /locus_tag="VFH96_09280"
     CDS             7366..9927
                     /gene="gyrA"
                     /locus_tag="VFH96_09280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET6974203.1"
                     /translation="METTSERNHINIEDEMRRSYLDYAMSVIIGRALPDIRDGLKPVH
                     RRVLWAMHELGNTYNKPYKKSARIVGDVIGKYHPHGDMPVYDTIVRLAQDFSMRYPLI
                     DGQGNFGSVDGDAAAAMRYTEIRMARLTNEILNDIEKETVDFQPNYDESLSEPKVLPT
                     RLPNLLINGSDGIAVGMATKIPPHNLTEIVDATLALLKDPEVTVQDLTKIVTGPDFPT
                     GGFIYGREEIRKSYEEGRGVLQIRARAGIDRIGRGAQERDAIVITEIPFQVNKARLLE
                     RIAELINEKKIDGISDLRDESDRTGMRIVVELKRDAVPQVVLNKLYKLTPMQSSFGVI
                     NLAIVNGQPRVLNLKQMLEAFVEFRREVVRKRTEYELRKAKARAHILEGLTKAIDALD
                     AIIFLIRNSRSVDEARTWLQGDLKTTAEVKSWKGIGSDVQIKTHLAKIQKTISSLAFS
                     EIQSQAILDLQLRRLSALERQKITDEYEQVIKLIAELEEILANERSLRRVIAKELEEV
                     KKDFGDGRRTEIVDEGVELSIEDLIADEDVAITVTNSGYIKRTPITTYSRQGRGGKGR
                     FGAAAKNGDFVEHLFIASTHAYLMIFTDDGMVYKLKVHEVPDAAASARGKAVVNLINI
                     PSDRRLAGVVPVREFAEGRYVVMVTRKGVIKKTSLADFQNIRTNGIIAINIDDGDQLL
                     DVVLTDGTKRIFIATHDGVAIRFDEKNVRPMGRATRGVRGIDLRKDDYVVSVCPVSAE
                     DTERMLSVSELGYGKQTSITTYRLQSRGGKGVINMKTTEKTGKVVAVFPVEDDAEIMI
                     ITQQGKLIRLEAADIRKTGRSAQGVRLIKTDAGDKVTSASLVEAAAEEETEETPG"
     gene            complement(9924..10598)
                     /locus_tag="VFH96_09285"
     CDS             complement(9924..10598)
                     /locus_tag="VFH96_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET6974204.1"
                     /translation="MTNIRVLFFGAARDAVEANQLDLVLEEPATVSSAFQSLKARFSK
                     LERFGRSLLFAVNQEYATPDTQLKENDELAVFPPVSGGSHDFFELTKEPIDVGEVARR
                     VVLPEFGATVTLDGYAREGTKGKRTLYLIYEAYDTMALTEMERLGVEAHKEFEIAHIG
                     IVHRTGRIDIGETSVVIAASAPHRQAAFQACEWAIKELKRTVPIWKKEVYEDGEAWVD
                     PRESAS"
BASE COUNT         2433 a         2872 c         3070 g         2383 t
ORIGIN      
        1 aattgttttc aatgctgccg tcgcagtttt cgccattggc acgctgcgac tacgcgaggc
       61 tacgggcgaa gtgttgccga agcacgagtt ttcattgcgg ccctttaaac gaatgacaag
      121 ctggacagct gcaaccacac gccgcaccac ggtacgcgcc aagaaggcgc gtcaacgtgt
      181 tcgcagtcgt gatgtgacat cagagttcaa gaagatcgac gtttcagaaa caccggataa
      241 gagggccgtc agttaattcc gattgatttg gccacaggtt tcgcggatta cgcagatcag
      301 tgcagatccg tgtaatctgt ggctgtttat tcatatgctc gaactaaatg gccagcaatt
      361 aacccttcag caaatcgctg ccgttgctca gggtcgggaa caggtcgttc tcgccgcaga
      421 ggcgcgtgct cgcgtcgaga aagctcgaca agtggtccaa aacatcgtta acgagggccg
      481 cactgtttac ggtgtcaaca cgggatttgg aaaactgtca gacgtgagta tcgaacagtc
      541 tgacttgagc cagttgcagt tgaacctggt gcgcagtcac tcatgtggat taggtcagcc
      601 gctgtcggaa gctgaagcac gtgcgatgtt gttgttacgc gcgaatgttt tagcggccgg
      661 attcagtggc gcgcgaccgc ttgtgatcga cacgctttgc gcgatgctgg aaaacggcgt
      721 cacgccggtt attcctgaaa aagggagcgt cggcgcgagt ggcgatctgg cgccgcttgc
      781 acatcttgcg ctcgccgcga ttggcgaagg cgaagttttc tacgagggtg aaagattatc
      841 agcgtccgaa gcactgaagc gcgcgaacgt cgcgccgttg cagttggaag taaaagaagg
      901 actggctcta ctcaacggaa cacaggcgat gggcgccgtc ggatcgctcg cgctcaatcg
      961 cgccgagaag cttgttcgac ttgccgacgt cgccggcgcg atgacgttgg aagcgttgaa
     1021 aggaacgccg gtcgcttttg atgaacgcat tcatgcagcg cgtccgcacc cggcgcagtt
     1081 agcagttgcc gcacacttgc gagagttgtt gcaggagagt gagattcgga aatcacatct
     1141 caacaacgac ccgcgcgtgc aggacgctta cagtttacgt tgcatgccgc aagtgcacgg
     1201 agcgattcgc acggcgttga gtcacgcgat ccaagtggtc gagattgaaa gtggtagcgc
     1261 gacggataac ccgctcgtct tttccgagac tggcgaagtg ttgtcaggcg gaaatttcca
     1321 cggcgcgccg ctcgcactcg tttttgacta cgctgcaatc gctttgacag acttgatgag
     1381 tatcactgag cgacgcatcg acaggctcgt aaatcctgac tcaaacgaag gactgccgcc
     1441 attcttgacg tcgcagcccg gaatctcgtc gggtttcatg atgttgcaag tgactgcggt
     1501 cgcgctgttg aatgaagcga aagtgctggc ccatccggcg agcatcgaca acgtgccgac
     1561 cgatggcgga aaagaagatc acgtctcgat ggggatgacc gcggcgacga agttacgcgg
     1621 aattgtcgaa ctcgccgagc tggcgacctc gatcgagttg ttaaccgcgg cgcaggcgtt
     1681 ggagtatcga tcgctcgcgc ccggacgcgg cgtgaagcag gcttacgaca tcgtgcgtaa
     1741 acttgttcaa ccgctgacac aggatcgatc gatggcgaaa gatattgagc gtcttcgaga
     1801 tgcaattacg gctggagaat ttgcgggctg ttaagctaga agtttgtttc acgcaaaggc
     1861 gcgaagttga cgcagagtcg caaagtatat tttgctctct ttgcgtcttt gcgtgaactt
     1921 tgcgtctttg cgtgaaaact attgggagga cttgtatggc gaacggaatg gatgaccgtc
     1981 gaaaaagttt ggaagaagaa tatttccatc gcaagaacaa ggaagcaatc gagaaactgc
     2041 gcgccaagat gaaggtggcc gaagaggcca aagcggccgg gctgtcttcg atgcagtgcc
     2101 cgcgttgtga cggcactttg aaggaaaaca agtttgagga agtgctgatc gacatttgcg
     2161 acaaatgtgg tggcgtgtgg ctcgattcag gtgaattgga acagttgacg aagaaagaca
     2221 ctggcggttg gttcagccgg tattggttct cagaaggatg agtcatcgcg ttattcacgc
     2281 ccctcatggg ccacagttaa gttgcaaagg ctggcatcag gaagccgcgc ttcgttgcct
     2341 gatgaacaat ctcgatccgg acgtcgccga acggccggac gaattgatcg tctacggcgg
     2401 cgccggcaag gctgcgcgta actgggccgc cttcgacgcg attgtccgcg agttacaggc
     2461 gcttgagaac gacgaaacgt tgttggtcca gtcgggcaaa ccagtcgccg tctttcgcac
     2521 tcacgaacac gcgccacgcg ttttgatcgc gaattcaaat cttgtcggcg cgtgggccaa
     2581 ctgggaacac ttccacgagc tcgaacgccg cggcctgatg atgtacggcc agatgaccgc
     2641 gggcagttgg atctacatcg ggacgcaggg aattgttcaa ggcacttttg agacgttcgc
     2701 ggcaatggct gaccggcaat tcggcggcga tctttccggc aagctcgttg tctcaggtgg
     2761 aatgggcggc atgggcggcg ctcaaccgtt agcggcgacg ttgaacggcg cggtgtttct
     2821 cggtatcgac gtcgatcctg agcgcatcga gcgacgtgtt cgcaccggct attgcgatcg
     2881 gatcgcgatg acgctcgacg aagcgctcga catctgtgaa gacgcacgca aacagaagcg
     2941 tggcgtttcc gttggactcg tcggcaattg cgccgagatt ctgccagagc tggttcggcg
     3001 cggcgttcat gtcgatgcgg ttacagatca aacgagcgca cacgatcctc tgaatggcta
     3061 cgtacccgcg agaatgtcac ttgatgacgc cgccgagctg cgcgcaagcg accccgctgc
     3121 gtacgtcgag aagtcgatgg cgtcgatggc tcagcacgtt gaagcgatgt tggcgttgaa
     3181 gcgtgcgggc gcaatcgcgt ttgattacgg caacaatatt cgcaagcagg cgtttgatgc
     3241 gggatgcaca gacgctttcg agataccagg attcgttccg gaatacattc gtccgctttt
     3301 ctgcgaaggc aaaggaccgt ttcgctgggt agcgttgtca ggcgatccag aggacattcg
     3361 caagaccgac gatctcgcgc tcgagatgtt tcctgacgat cgcacgttga atcgttggct
     3421 gcgtctggcg cgcgagcgcg tccacttcca gggattgccg gcccgcatct gttggctcgg
     3481 ttatggtgaa cgagctgaga tgggcgaggc gattaacgat ctcgtcaagc gtggcgacct
     3541 caaagcgccg atcgcgattg gacgcgatca tctcgacacc ggatcggtgg cgtcgccgtt
     3601 tcgtgaaaca gaaaatatga aagacggatc agatgcagtg gccgattggc ccatcctgaa
     3661 cgcgttgctc aacacggcga gtggcgccag ttgggtatcg tttcatcacg gtggtggggt
     3721 cggaattggt tattcgctgc atgccggtca ggtgtccgtc gccgatggaa ctgatgaggc
     3781 ggcgaagcga ttgaaccgtg ttttgacgaa cgatccgggg cttggtgtgg cgcgacatgt
     3841 tgatgccggt tatgaagagg ctgaaaacac cgcgcgcgag aagagtatta acattccgct
     3901 actcgccggt taattgggac aggatttaca agatgtttca agattcatcc tgaaaaaatc
     3961 ttgtaagtcc tgtccaacat ccgcctatga aaacaacaac tcgattggtc cgagcgcttc
     4021 tgctcatctt ttgctttgcg acagtagtaa gtgcacgtgt cgatacggtt cgctttcaaa
     4081 gcaaactgat caacgcaacg cttccttaca acgtcattct gccaaccgat tacgacacgt
     4141 caaagaccac tcgctatccg gtgctctacc tgcttcacgg tctgaccggt cattactcgg
     4201 attgggtctc gcgcacaaat gtcgccgact acgcttccga atatcgcctg atcgtagtca
     4261 cgcctgaagg caacaacagt tggtacattg acagcgtcgg cgtcgcgaac gacaaatacg
     4321 agagctacgt actcaatgaa ctgatccctg acgtacagca gcgctatcgc acgatcgaag
     4381 ctcgttacgg ccgctccatc gcgggactct cgatgggtgg ttacggcgca ttcaagtttg
     4441 gcttgaagac gccggcgact tttgtctttg ccggatcgat gagtggtgct tttggcgtga
     4501 cgcgcttcac cgagaaagaa atcggccctg aacgctttgc agattcgctg aaactgttcg
     4561 gcgctctcgg cagcgacacc cgtgcggcca acgatcttta cgacatcatc ggcaaactga
     4621 ctcccgcacg ggttgcgtcg ttaccatatt tctactttga ctgcggcact gaagacgcgc
     4681 cgctaatctt ccccttcaac cgcgagctcg cggccctcat gtttgaaaag aaaataccgc
     4741 acgagtttcg agaattaccc ggcgatcact catggggcta ctgggatcga caggtgcggg
     4801 aaatcttgag gattgccaca gacaaaatgc ggatgccgtc gacgaagagg atttcgcgtg
     4861 cttaattttg ccatcgatgt cgcccgtgac gcgggctcgc tgctggtcca acgactcgga
     4921 tcggcgaagg tcagcaccaa aggcgatatc aacctcgtca ccgaagccga cattgctgct
     4981 gaaaatctga tcatcgatcg aattcgttct cactatccac aacacggaat tcttgccgaa
     5041 gagtcaggcg aagcagtttt ggtgggcggc aagcgcagcg agtggaagtg gatcatcgat
     5101 ccgttggacg gcacgacgaa ttacgcgcat ggatatccgt gcttctgtgt ttcgatcgcc
     5161 ctcgagcgat cgggcgtgct cgagatcggc gttgtttacg atccgatgcg caacgagatg
     5221 tttgcggctg aacgtggtca gggcgcaacg ttgaatgatc gccagattcg tgtttcgtca
     5281 gtggaggagt tgagtggtgc gatgttgtgc accggatttc cgtacaacgt gcgtgagcgt
     5341 ccggacttcg cgcgtggatt cacaaacttc acgatgaatg cgcaagcagt gcgtcgcgat
     5401 ggatcggcgg cgctcgatct ggcgtacgtg gcgtgtggtc gctttgatgg tttctgggaa
     5461 gacggtctca gcccctggga cgtcgccgct ggcgcggtat tgatcagtga agcgcgcggc
     5521 aggattacga atttcaaaga cgagccggtg aatatttaca acgagcagat actggcgagt
     5581 aatggactca tccacgacgc catgatgcgt gtgttgagtt aaaagcattt tcacgcaaag
     5641 gcgcaaagaa gtctcaaatt cttctttgcg tctttgcgtg aaattttctt cagagcgccg
     5701 aagcaataac ctgagcaagc ttctcgcttt cacgtcgcgc gcacgcgcgt gtttcttcgt
     5761 tgggacattg ctcggcgtgg tggagcggat gataggtcac tacgactttt ccgggtctcg
     5821 gaaaacgcca gcctcgaggc caaacacggt ttccaccctt aatcgttacc ggcaaaatcg
     5881 gcacctgcgc ttccatcgcc aatcgcaccg cgccgacttt gaatcgatcg agatctccag
     5941 acacggtact ccgcgcgccc tcaggaaaaa tcaccaccgc gcccccatta cgcaagaact
     6001 gtaacgaact ccgaatcgcc gccggatcgc ttccttccaa tgcgatgggc cacgctccaa
     6061 accagatcaa aaatctccca accaccggcc aacgaaacgc cgcactccac gccagatagc
     6121 gcgtcggtcg cttgattgga accgaaagcc agaacggatc gatatacgtc tgatgatttg
     6181 cagcgatgat caaaccacca ttcttcggca cattctcaag tccattgaac tcaatcttga
     6241 agaaaacacg actcaacgcg ccgacgacgg gccggattga atcaagcgcc cactgcggca
     6301 acggcgagat ctcgggcgat gctcgcggat cttctctctt gactcgtgct gtgccgttgt
     6361 tactcaatgt tgttagctgt tcgcgctctg tttatcttac aacggccatt ccttatctat
     6421 gaactccacg tgaattcttg gaatgccgtc ggcgccggta ctgcgcgagc gaatggcagc
     6481 gtgggcggtg atgccgtgct gctcgctggt gagtcgaata aaacgtcctt ttgggatttc
     6541 gagcgtggtg aagagtgtcg ctcctttggt gctgatgtct tcggtgacag tgtattcact
     6601 tgcgaccacg gcaccgtcga tatccaccaa ctccactcgc atatcgaccg cgatattgtg
     6661 gcgggtgtaa gcgcgctgat ccattgaagg aagaggaatc aaaatctcgt cagcagccga
     6721 gagtgcctgg aatgcagtcg ttgagatgtc gtaacgcttc catggctctg tttcaaagct
     6781 tgccgggggt cgcttgccga tgaaggcgac gccaatttca aacacagggc ctttttcgcc
     6841 gacagaagcc ttcatatacc gcacgatggc ccagattcgg tattgatctt caacatgatc
     6901 gaacactcgc aattgccttg gcatcgggat cgtcatgaac aacaaccggc ctttttcagt
     6961 gggacgtttg agcgtaaaac ccgcgccgaa gggagttacg ttggtgagtc tggtgatctc
     7021 agtccactcg aaatcgggcg tctcacgcac ttgcacgcgc accggcagct gcagtgccaa
     7081 tcgctcacga attcgtttcc aatttcgctc ttcactcatg ggagccgact gacgtgggaa
     7141 gtcgtctaaa acggtcggga gtgcctacac gaagctattg attctattag agtttacgcc
     7201 agtttccgcg gttgctaatt gggctcaaaa aggctaaaat acgggctact ttgatcgtga
     7261 tgttccaccc gtttttcttc tccgaaaaag cgccaaactc aagctcggtt tctcttcaaa
     7321 caggggccag actgcgtggc gggattgtac tggaaaggct tttagatgga aacaacttct
     7381 gaacgcaatc atataaacat cgaagacgag atgcggcgct cgtatctcga ctacgcgatg
     7441 tcggtaatca tcggccgcgc gctgccggat attcgcgacg gattgaaacc cgtgcaccgg
     7501 cgcgtgttgt gggcaatgca cgaactcggc aacacataca acaagccgta caagaagagc
     7561 gcgcgcatcg tcggcgacgt catcggtaaa tatcacccgc acggcgacat gccggtgtac
     7621 gacacgatcg ttcgtctcgc gcaggatttc tcgatgcgtt atccgcttat cgacggccag
     7681 ggaaacttcg gctcagttga cggcgatgcg gccgctgcga tgcgttacac ggaaatacgc
     7741 atggcgcgtt tgacgaatga aattctcaac gatatcgaaa aagagaccgt tgactttcaa
     7801 ccaaactacg acgagtcgct cagtgaacca aaagtcttac cgacgcggct tccgaatttg
     7861 cttatcaacg gttctgacgg catcgcggtt ggaatggcga cgaaaattcc gccacacaat
     7921 ctcacagaga tcgtcgatgc gacactcgcg ttgttgaaag atccagaggt tacggttcag
     7981 gacctgacga agatcgtgac tggccccgat tttcccaccg gtggatttat ctacggtcgc
     8041 gaagagatca gaaagtcgta tgaagaaggg cgcggcgttt tgcagatacg cgcgcgcgcc
     8101 ggcatcgacc ggattggtcg tggcgcacag gaacgcgatg caatcgtcat caccgagatt
     8161 ccgtttcagg tcaacaaagc tcgtttgctc gagcgcattg ctgaactgat caacgagaag
     8221 aagatcgacg gtatttcgga tctgcgcgac gagtcagatc gcaccggcat gcgcatcgtc
     8281 gtcgagctca aacgcgatgc ggtgccgcaa gtggtgctga acaagctcta taagttgacg
     8341 ccgatgcaat cgtcgtttgg tgtcatcaac ctggcgatcg tcaatggaca gccacgagtt
     8401 ctgaatctca agcagatgct cgaggcattc gtcgagtttc gccgcgaggt ggtgcgcaaa
     8461 cgcaccgagt atgagctgcg caaagcaaaa gcgcgcgcgc atattctcga aggtctcacg
     8521 aaggctatcg atgcgcttga tgccattatc tttctcatcc gtaactcgcg ttcggttgac
     8581 gaagcccgaa cgtggcttca gggcgatttg aagaccacgg ctgaagttaa gtcgtggaag
     8641 ggtattggct ctgacgttca gatcaagact catctcgcga agatccaaaa gacgatctcg
     8701 agtctcgcgt tttccgaaat ccagtcacag gcaattctcg acctgcaact gcgtcgactc
     8761 tcagctctcg aacgacagaa gatcaccgac gagtacgagc aggtgatcaa gcttatcgcg
     8821 gagctcgaag agatcctcgc caacgagcgc tcgttgcgac gcgtgattgc gaaagagctc
     8881 gaagaggtca agaaagattt tggcgacgga aggcgcaccg agattgtcga cgagggcgtc
     8941 gaactctcga tcgaagatct catcgccgat gaggacgttg cgatcaccgt caccaacagt
     9001 ggttacatca agcgcacgcc gatcacgact tactcgcgtc agggacgcgg tggcaaaggc
     9061 cgtttcggcg cggcggcgaa gaatggcgac tttgtcgagc atctcttcat cgcttcgacg
     9121 cacgcgtatc tgatgatctt cactgacgac ggcatggtct ataagttgaa ggttcacgag
     9181 gtgccggatg ctgctgcttc ggctcgcggc aaggcggttg tgaatctcat caacattcct
     9241 tccgatcgtc gtctcgcagg cgttgttccg gtgcgtgaat ttgcggaagg tcgttacgtc
     9301 gtgatggtca cgcgcaaggg cgtgatcaag aagacgtcgc tggctgattt ccagaacatt
     9361 cgtaccaatg gaatcatcgc gatcaacatt gacgatggcg atcagttgct cgacgtggtg
     9421 ttgactgacg gtacgaagcg cattttcatt gccacgcacg acggcgttgc gattcgtttt
     9481 gacgagaaga acgtacgccc gatgggacgt gcgacgcgcg gtgtgcgtgg catcgatctc
     9541 cgcaaggatg actacgttgt ttcagtttgc ccggtttccg cggaagacac ggagcggatg
     9601 ttgtccgttt ccgaactcgg ctacggcaag caaacgtcga tcacgaccta tcgcttgcag
     9661 tcgcgtggtg gtaaaggcgt cattaacatg aagacgactg aaaagaccgg caaggtagtg
     9721 gcggtgttcc cggtcgaaga tgatgcggag atcatgatca tcacgcagca aggcaagttg
     9781 atccgccttg aagccgctga catccgcaag acgggccgca gcgctcaagg ggtgcgtcta
     9841 atcaagaccg acgccggcga caaagtcacc agcgcctctc tcgtagaagc tgctgctgag
     9901 gaagaaacgg aagaaacacc gggttaggac gcggattcac gcggatcaac ccaagcttcg
     9961 ccatcctcgt acacttcttt tttccagatg gggacggtgc gtttgagttc tttgatggcc
    10021 cactcgcagg cctgaaaggc ggcctgacga tgtggcgcgc tggcggcgat gacgacgctt
    10081 gtttcgccga tgtcgatgcg gccggtgcgg tggacgatgc cgatgtgggc gatctcgaat
    10141 tccttgtgcg cttccacacc gaggcgctcc atctccgtta acgccatcgt gtcgtaagct
    10201 tcatagatca agtacagcgt tcgtttgcct ttggtcccct cacgcgcata accatcgagt
    10261 gtcacagtag cgccgaattc cggcaacacc acgcgccgcg ccacctcgcc cacgtcgatc
    10321 ggctctttcg tcaattcgaa gaagtcgtgc gatccaccac tcaccggcgg aaacacagcc
    10381 aattcatcat tctctttcaa ctgcgtatcc ggcgtggcgt attcctgatt caccgcaaac
    10441 aacaacgacc gcccaaaacg ctcgagcttc gaaaaccgcg ccttcaaact ctgaaacgca
    10501 ctcgataccg tagccggctc ctcgagcacc aaatccagtt ggttagcctc aaccgcatcg
    10561 cgagctgcac caaaaaacaa cacacgaatg ttcgtcattc aatctcaatc cttttaccgc
    10621 catgcccatt gctcttggca agctcgtgaa tccgcgcccg ctcctcgtac cctttgaccg
    10681 cgatgctcaa catcgatcgc aaccgctcca accgttccga agttgaacga agctccagca
    10741 tctcctgttt gacttcgg
//
LOCUS       DASSFQ010000256         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1311964, whole genome shotgun sequence.
ACCESSION   DASSFQ010000256 DASSFQ010000000
VERSION     DASSFQ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311964"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFH96_09290"
     CDS             complement(<1..754)
                     /locus_tag="VFH96_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6974205.1"
                     /translation="MSTGRRILRSIGFLLAGSLLMLVVAIPTLFLARRFYSEVIGSWI
                     GRTVLRIWGINYTTHYSEPLPNVQTIYLSNHTSTLDVMLLIALALPRARFFLSGYLRK
                     FPVVLIIGYAIRVFWTVPQKFQERRREIFQRADRILRRTGDSVYLSPEGKCVTTGEIG
                     AFNKGAFHLATSLKAPIVPIYIHIPPTVQGWRGYHAKPGTVDVYFLPAIDTSRWRLED
                     LEANRDRVRSLYLRVHDAIRADGKLPADLGIET"
     gene            complement(757..1746)
                     /locus_tag="VFH96_09295"
     CDS             complement(757..1746)
                     /locus_tag="VFH96_09295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6974206.1"
                     /translation="MIAEAEILPMTALLESGPPSTHFERVPCYFCQATECATFLTAQD
                     DLTGRPGTFTFITCARCGLRYQNPRLGIEHVKDFYDQEYIAHRKKASWGLLTGFFNWV
                     MDRHDRQKEKLVRRYVKLGTESEVLDVGCAVGTFLQKVRTLYGARVVGVDFKDLRSCP
                     SLEGVEFHCGLFYEQPLERERFDAITMWHFLEHDYDPLRTLETARDLLKESGYLVIEV
                     PRLDSLTFRLYGNRWPGLQAPQHTVLYDREMLLRMVKKAGLDVVDYLPYGAFPAFFYF
                     FAGAAFKLLKGKGLNLSRAIYPYFIGQVLFAPILAFEKHLNFAMQTVVCKRES"
     gene            complement(1743..2408)
                     /locus_tag="VFH96_09300"
     CDS             complement(1743..2408)
                     /locus_tag="VFH96_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974207.1"
                     /translation="MFSTKLILAYWRERYPVSVFVPFAILIAAAGIAAGGSLPTARDV
                     ITSCVLAYTLILVFRILDDLADLTNDRRRYPSRVLVQSSDLTPIMVLAFLIAAADVLI
                     ILWQPQRALRLIVLAAVSIFLALWYLLRMRFRAGPLVSAHLILIKYPVISLLTCTNWE
                     RLTVNTALPLFGTVYLGICIYEQLHDRAVRESRGGSWVFAAEAGLLACLPLLMLLTGG
                     FLR"
     gene            complement(2371..>4106)
                     /locus_tag="VFH96_09305"
     CDS             complement(2371..>4106)
                     /locus_tag="VFH96_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HET6974208.1"
                     /translation="WSDANVNENFPAPISPFLYSFASAGYANYFRNLARAFGLSRTRI
                     AAMDDALRHIIGVHGARMYYNLTNIHTVLRTAPFGDALVAAFNNFVGSGGSFGKVARA
                     KTSRTREVFELAVIAAKVTWLYLFLGRRVASFERTVDDFAERTEPHRLATLSLRELRL
                     LLNELMDIRCHRWTNASLADAAAMVSYAVLRRLVEQADGGKAPKALHNTLLKAIPQLV
                     SSEPVQQLWELSRAVRADAALHRLFQGDAESAVNQINVKPEFAGFRAAFDDYLQRWGF
                     RCSAELMLTAPSYQEQPALLVETIRAYAQRDTESPAAALSRQLAERKEETRRLLRKMR
                     GRTVVLRTVLRWTQAAIACRERARLKQALLYSRCRRLALALGDQFARRGHLAERDDIF
                     WLTVSEVDELAAGNAMFPHHVQELTAMRKRAHEQLSATQPGNDFILREGEYLPLWGEL
                     RPVALENDNTGGSRTLRGVGACGGRAVGRAIVLHDASEAGRLAADDILITRQTDPGWA
                     SVFFLIKGLVIERGGMLSHGAIVAREFGIPCVVGVPHATRELSQAGQIEVDGDQGEVH
                     VLDEIDSRLLA"
BASE COUNT          962 a         1132 c         1188 g          824 t
ORIGIN      
        1 gcgtctcgat gccgagatcc gccggaagct ttccgtctgc gcggatcgcg tcgtgcacac
       61 gtaagtaaag actgcgtact cgatctcggt tggcttcaag atcctcaagt cgccagcgcg
      121 aggtgtcgat cgccggaagg aaatagacgt cgactgtccc tggcttcgcg tggtaacccc
      181 gccaaccctg cactgttggc ggaatgtgaa tgtagatcgg aacgatcggc gccttgaggc
      241 tcgtggcgag atgaaatgct cctttattaa aggcgccgat ttcgcccgtg gtgacacact
      301 ttccttccgg actgaggtac accgaatcac ccgtgcggcg cagaattcga tccgcacgct
      361 ggaagatctc gcgacgccgc tcctgaaact tttggggcac ggtccagaaa acacggattg
      421 cgtaaccgat gatcaagacg accgggaatt tgcgcaggta accggagaga aaaaatcgcg
      481 cgcgtggtaa tgcgagtgct atcaatagca ttacatccaa ggtcgatgtg tggttcgaaa
      541 ggtagatcgt ctggacgttg ggaaggggtt cggaatagtg tgtggtgtag ttgatgcccc
      601 agatccgaag tacggtgcgg ccgatccagc tgccgatgac ttctgaataa aaacggcgtg
      661 cgagaaaaag cgtgggtatc gcgactacca gcatcaacaa cgaaccggct agtagaaatc
      721 cgatcgaacg caaaatacgt ctacccgtcg acatacttaa ctctcccgct tgcagacgac
      781 ggtctgcatc gcaaagttga ggtgcttttc gaacgcgaga atcggtgcaa agagaacctg
      841 cccgataaaa tagggataga tcgcgcgcga taggttgaga cccttgccct ttaacagctt
      901 gaatgctgct ccggcaaaga aataaaaaaa cgctggaaaa gcgccgtagg gcaaatagtc
      961 gacgacgtca agcccggcct tcttgaccat acgcaaaagc atttcgcggt cgtaaagaac
     1021 tgtatgctgc ggcgcctgca gaccaggcca ccggttgcca taaagtcgga acgtaagcga
     1081 gtcgagccgt ggaacctcaa tcacgagata gcctgactct ttcagcagat cgcgggctgt
     1141 ctcgagtgtg cgtaagggat cgtaatcgtg ttcgaggaag tgccacatgg tgatcgcgtc
     1201 gaagcgctcg cgctcaagcg gctgttcgta aaacaaaccg cagtgaaact ccacgccttc
     1261 gagcgacggg cagctgcgta gatccttaaa atcgacacca acaacacgag cgccatagag
     1321 tgtgcgcact ttctgcaaaa acgttccgac cgcacaaccg acgtcgagca cctcgctctc
     1381 cgtaccgagc ttgacgtatc gacggacgag tttttctttc tgccggtcat gtcgatccat
     1441 gacccagttg aagaaacccg taagcaagcc ccaactcgcc tttttgcggt gggcgatgta
     1501 ttcctgatcg tagaagtctt tgacgtgttc aattccgagt cgtggattct gatacctgag
     1561 tccgcagcgc gcgcaggtga taaaggtgaa cgttcccggc cgaccggtga gatcgtcctg
     1621 cgctgtcagg aaggttgcgc attccgtcgc ctggcagaag taacatggaa ctcgctcaaa
     1681 gtgcgtagag ggtggaccgg actcgagtag tgcagtcatc ggcaggattt cggcttcggc
     1741 gatcatcgca ggaaacctcc agtcaaaagc atcaacagcg gtaggcaggc tagtagtccc
     1801 gcctccgcgg cgaagaccca cgagccgccg cgcgattcgc gcaccgcacg atcatgaagc
     1861 tgctcgtaaa tgcatattcc gagataaacg gtgccgaaca acggaagcgc cgtgtttacc
     1921 gtcagacgct cccagttcgt gcaagtcaaa agagaaataa ccggatactt aatgagtatg
     1981 aggtgcgcac tgacgagcgg accggcgcga aagcgcattc gcaggagata ccaaagcgca
     2041 agaaagatcg aaaccgcagc aagaacaatg agtcgcagcg cgcgctgcgg ctgccaaaga
     2101 attatcaaga catcggcggc tgcgatcaaa aacgcaagca ccatgatcgg tgtcaggtcc
     2161 gacgactgga ccaacactct gctgggatag cgccgccgat cgttggtgag atcggcgaga
     2221 tcatcgagga tgcggaaaac caaaatcaac gtataggcga ggacacaact tgttatcacg
     2281 tctcgagctg tcggcagcga acctcccgct gcgattcccg cagccgcgat caagattgcg
     2341 aatggcacga agacgcttac cggataacgc tcacgccagt aagcgagaat caatttcgtc
     2401 gagaacatga acttctccct gatcgccgtc gacttcgatt tgtcctgcct gcgacaactc
     2461 acgagttgca tgcggtacgc cgacgacgca tggaattccg aattcgcgcg cgacaattgc
     2521 tccgtgtgaa agcattcctc ctcgttcaat caccaggccc ttgatcagaa aaaagactga
     2581 cgcccagcct gggtctgttt gacgtgtgat caaaatatcg tccgcggcga ggcggcccgc
     2641 ctcactcgca tcgtgtagaa cgatcgcccg acccacggcg cgaccgccgc aggcgccaac
     2701 acctcgcaga gtgcgcgaac cgcccgtgtt gtcgttttcg agtgcgactg gacgtaactc
     2761 tccccagaga ggcagatatt ctccttcgcg aaggatgaaa tcattccctg gttgggttgc
     2821 ggagagttgt tcgtgcgcgc gcttgcgcat ggccgtcagt tcctgcacgt gatgtgggaa
     2881 cattgcgttt cctgcggcta gctcgtcgac ctcggaaaca gtcaaccaga aaatgtcgtc
     2941 gcgctccgct aaatgtccgc gtcgtgcaaa ctggtcgcct agagccagcg caagtcggcg
     3001 acaacggctg tacaaaaggg cctgtttcag gcgcgctcgt tctcgacaag caatggcggc
     3061 ttgcgtccaa cggagcactg ttcgtaagac gacggtgcgg cctcgcattt ttcgcagcaa
     3121 gcgccgagtt tcttccttcc gctctgcaag ttggcggctg agtgccgcag ccggcgattc
     3181 ggtatcgcgc tgcgcataag cacgaatcgt ttcgaccagc agtgccggtt gttcctggta
     3241 gctcggagcc gtgagcatga gttcggcgga acagcgaaag ccccaacgtt gcaaatagtc
     3301 atcgaacgca gccctaaagc ctgcgaactc cggtttgacg ttgatctggt taacggcaga
     3361 ctccgcatct ccttgaaata acctgtgaag agctgcgtct gcgcgaacag cccgcgagag
     3421 ttcccagagc tgctgcacgg gctcactact gacgagctgt ggaatggctt tgagaagggt
     3481 gttgtgaagt gccttcggag cttttccgcc atcggcttgc tcgaccaaac gtcgaagcac
     3541 ggcgtaactg accatcgcag cggcatcggc gaggctcgca ttggtccagc gatggcagcg
     3601 gatgtccatt agttcgttga gcagtagccg aagctcgcgc agcgagaggg ttgctaaccg
     3661 gtgtggttcc gtgcgctcgg caaaatcgtc gacggttcgt tcaaacgatg ccacgcgtcg
     3721 tccgagaaaa aggtacagcc aggtgacctt tgccgcgatc acggccagct cgaaaacctc
     3781 gcgtgtacga ctggttttgg cgcgtgccac ctttccgaac gaaccgcctg atccgacgaa
     3841 gttgttgaag gcggcaacca gtgcgtcgcc gaagggcgcc gttcgcaaca ccgtgtggat
     3901 gtttgtcagg ttgtaataca tcctcgcgcc atggacgccg atgatgtgac ggagcgcgtc
     3961 gtccatagcg gcgattcgcg ttcttgagag tccgaaggca cgggccagat tccgaaagta
     4021 gttggcgtaa ccagcgctcg cgaacgagta aaggaaaggt gagatgggcg ccgggaaatt
     4081 ttcgtttacg ttggcgtctg accagc
//
LOCUS       DASSFQ010000257         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_270998, whole genome shotgun sequence.
ACCESSION   DASSFQ010000257 DASSFQ010000000
VERSION     DASSFQ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270998"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFH96_09310"
     CDS             <1..865
                     /locus_tag="VFH96_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET6974209.1"
                     /translation="RTAAGRAFATGFMIFGVGVVLYALTSTVQSIVHSELFARYGHSR
                     KMSKLRDHFIICGAGRVGSHLIRSLRAADGIFLVIESDQRKCEALMDMGIPVLIRDAT
                     IEESLIEAGVEHARGLASCLPDDADNVYVVLTARDLNPNIHIVARAAEEQAESKLIRA
                     GANRVVAPTIIGGHRMAMALTKPAVGDFLDSVTANHLELGFEQLEVDPISTLVNRKLS
                     ETVIRSELNIVIVSIRRNNGEIIFNPSGETKIEGGDMLIAIGNAESLARLNALARGRA
                     TQQRAIEAQTR"
     gene            862..1704
                     /locus_tag="VFH96_09315"
     CDS             862..1704
                     /locus_tag="VFH96_09315"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6974210.1"
                     /translation="MKVLVTGGSGYLGTFVRSFFSADDFSRRSGFDILDYQAVERVAD
                     YDAVIHLAAHFDKSPDGAFECFRTNAEGTANVIRNMKPGSTFIYASTKDVYGSNAGEI
                     DTVNEDTSTNYCGQTAFEWSKLIGERYVEYYARTKNIRACIFRLSTVYARPSEDNAPG
                     LVTHYVESVKNRQPIRLPAGVDPVRDIMYVDDFSRACQAFIGLKHEFGLYNLGGGSMN
                     AISLRDILKRVSAIIEIEPVIQEDPSMPAPAPLRYVSDISRAVDDLGWRPWMGIDAGL
                     REIL"
     gene            1705..2799
                     /gene="waaF"
                     /locus_tag="VFH96_09320"
     CDS             1705..2799
                     /gene="waaF"
                     /locus_tag="VFH96_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET6974211.1"
                     /translation="MNVQAESIKRVVVRGTNWVGDSVMTVPALRALRRVLPQAHITLV
                     IRPGTKGIFSEADFIDDVLVYDRKGALSVLGQVREWRRRQFDLAVLFQNAFEAALIPF
                     LAGVPLRLGYATESRQALLTHPLPLPDWRSAKHEVFYYLYLVTALEQMLFGRSDVCES
                     EPDASIQISESRKAEAAELLRANGVGESEPVVAICPGSINSRAKRWPAERFAALADRL
                     IESRRKVLLIGSKGELDVSEEVVRRMQNQPVVLTGKTSLDQITAVLDRADLIVTNDTG
                     PAHIGAALGRPTIVIFGPTNPLTTRPFAPEAEVLRHPPDCAPCMLRDCPIDHRCMTAI
                     TVDEVFEQSHALLKRGSFAKTVRSLCDSVA"
BASE COUNT          596 a          738 c          891 g          670 t
ORIGIN      
        1 gcgcactgcg gcgggccggg ccttcgcgac gggtttcatg attttcggcg tcggcgttgt
       61 gctttatgct ttgaccagca ccgtgcagtc gattgtgcat tcagagctgt ttgccaggta
      121 tggccattcg agaaaaatga gcaaactgcg agaccatttt atcatctgcg gtgcgggccg
      181 cgtcggctcg catctgatac gaagtctgcg agccgccgat ggcatttttc tggtgatcga
      241 gagcgaccag cgcaagtgtg aagccctcat ggatatgggc attcccgtgc tcattcgcga
      301 tgcgacgatc gaagagagtt tgatcgaagc cggcgtggaa cacgcgcgcg gtctggcctc
      361 gtgccttccc gatgacgccg acaacgtcta cgtagttttg actgcgcgtg acttgaatcc
      421 gaatattcac atcgtcgctc gcgctgcgga agaacaggca gagtcgaagt tgatccgcgc
      481 cggtgcaaat cgtgtggtag cgccgacgat catcggtggg caccggatgg cgatggcgtt
      541 gacgaaaccc gctgtcggcg attttctcga ctccgttacg gcgaatcacc tcgagcttgg
      601 tttcgagcag cttgaagtag atccaatttc caccctcgtg aaccgaaagc tgagtgagac
      661 ggtcatccgc tcagagctga acatcgtcat cgtcagtatt cgtcgcaaca acggtgagat
      721 catcttcaat cccagcggcg aaaccaaaat cgaaggtgga gacatgctta tcgctatcgg
      781 caacgcggaa tcgctggcgc gattgaacgc ccttgcacga ggtcgcgcga cacaacaacg
      841 agcaatcgag gcacagacga gatgaaggtt ctggttactg gtggcagtgg ttatctgggc
      901 acgtttgtcc gcagcttttt ttcagccgat gatttttcgc gccgttcggg tttcgacatt
      961 ctcgattatc aagcggtcga gcgggttgca gactacgatg cggtgattca tctcgccgca
     1021 cactttgata aaagtcctga cggggcgttt gagtgtttcc gcacaaatgc tgaaggcaca
     1081 gcgaatgtca ttcggaacat gaagccgggg tctacgttta tttatgcctc gacgaaggac
     1141 gtttacggta gtaacgccgg cgagattgac accgttaacg aagacacgag cacaaactac
     1201 tgcgggcaaa cggcgttcga gtggtccaag ctcatcggcg aacgctatgt cgagtattac
     1261 gcacgcacga agaatattcg tgcctgcatc ttccggctct cgaccgtcta cgcgcggccg
     1321 tcggaagaca acgcgcccgg tttggtcacg cactacgtgg aatcggtgaa gaaccgccag
     1381 ccgatcagac tgcccgccgg agtcgatccg gttcgcgaca tcatgtatgt cgacgacttt
     1441 tccagagcgt gccaggcgtt tatcggtttg aaacatgagt tcggtttgta caacctcggc
     1501 ggcggcagta tgaacgcgat cagtttgcgc gatattctca aacgcgtgag tgcgattatt
     1561 gagattgagc ctgtgatcca ggaagatccg tccatgccgg caccggcgcc tttgcgttat
     1621 gtttctgaca tctcgcgggc cgtcgatgat ttgggctggc gtccgtggat gggaattgat
     1681 gccgggctgc gagaaattct gtagatgaac gtgcaggcgg aaagtattaa gcgagtcgtc
     1741 gtgcgcggga cgaattgggt cggcgactcg gtgatgactg tgccggctct gcgcgcgctg
     1801 cggcgtgtgt tgcctcaggc tcatatcacg ctggtgattc gtcccggcac gaaaggaatt
     1861 ttttccgaag ccgatttcat tgacgacgtg ctcgtttacg atcgcaaggg agcgttgtcg
     1921 gtgctgggcc aggtgcgaga gtggagacgc agacagtttg acctcgcggt gttgtttcaa
     1981 aacgcgttcg aggcagcgtt gattcccttt ctggcgggcg tgccgctgcg gctcggctat
     2041 gcgactgaat cacgacaggc gctgctgaca caccctttgc cattgcctga ctggcgttcg
     2101 gcaaaacacg aggtcttcta ttatttgtat ctagtaaccg cgctcgagca gatgttgttt
     2161 ggccggagcg acgtttgcga aagtgagccg gatgcgagca tacagatctc ggaaagcaga
     2221 aaggcggaag cagcggagtt gcttcgtgcg aacggcgtcg gcgaatcaga acctgtggtc
     2281 gcgatctgtc cgggatcgat caacagtcgc gcgaagcgtt ggcctgcgga aaggtttgcg
     2341 gcgctcgcgg atcgtttgat cgagtcgcgc cggaaagttt tattaatcgg atcgaagggc
     2401 gagctcgacg tttcggaaga ggttgtgcgg cgcatgcaaa accagccggt ggtgctgact
     2461 ggaaagacga gtctcgatca gatcacggca gtgctcgatc gtgcggatct gatcgttacc
     2521 aacgataccg gaccggcgca catcggcgcg gcgctcggac gtccaaccat cgtgatcttt
     2581 ggtccaacga atcccttaac tactcgaccg ttcgctccgg aggcggaggt cctgagacat
     2641 ccgccggatt gtgcgccgtg tatgctgcgt gattgcccga tcgatcatcg ctgcatgacg
     2701 gcgatcacgg ttgatgaagt gtttgagcaa tcgcatgcgt tgttgaagcg cggcagtttc
     2761 gccaaaacag taaggagttt gtgcgactca gtcgcgtgag catgcagatg aagcgtccgg
     2821 cagtttttat cgatcgcgat ggcacgattt ccgaggaggt tggttacgtg aatcatcctt
     2881 cacggtttcg tgtgt
//
LOCUS       DASSFQ010000258         4738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2067242, whole genome shotgun sequence.
ACCESSION   DASSFQ010000258 DASSFQ010000000
VERSION     DASSFQ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067242"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /gene="pepT"
                     /locus_tag="VFH96_09325"
     CDS             <1..1276
                     /gene="pepT"
                     /locus_tag="VFH96_09325"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET6974212.1"
                     /translation="IGVASAQGQLKPPTEPVMDRFLRYVKIDTQSQEDQTTTPSTRKQ
                     LNLANLLAKELTALGAENVRVSEFGIVYATVPGNLPDNSKVPVVGLIAHMDTSPAVSG
                     ENVNPIIHKNYQGGDITLPKDPTQVITVEKNPVLKSLIGDDIITADGTTLLGSDDKSG
                     CAEIMTMIDILRQNPQLKHGTLAIAFTPDEEVGTGIEKFDVKGFGAKVAYTVDGEELG
                     EISDETWSARTATVTFQGKSTHPGTAKGIMINAMYAIADYLDRFPHDMLPETTEGRVG
                     FVHPYAGVIDVETSNLKILLRDFDMSGLDAKEKLLRDMVAQTAAKFPQVKITIDVKEN
                     YKNMKEVLKNYPELTTNAMEAAKRAGLKPFLKPIRGGTDGSRLTFQGLPTPNLFTGGG
                     NFHGKLEFNSRKGLEKSTETLVNLVQIFAEKS"
     gene            1308..2198
                     /gene="era"
                     /locus_tag="VFH96_09330"
     CDS             1308..2198
                     /gene="era"
                     /locus_tag="VFH96_09330"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET6974213.1"
                     /translation="MTHAGYVALIGRPNAGKSTLLNQLVGQKIAAVSDKPQTTRFRIQ
                     GVITKPEGQIVFVDTPGVHQPGYELNRRMMAAVQDALMGVDLVCLIRDVSVSTGNGDR
                     FVLDLVKRSDKPALLLLNKIDKLEEKSALLPLIEWYSKEHDWQEIVPISALKQDQTDV
                     LIESCLRHLPEGEPIFAEDELTDQSLRVLVAEIVREKVLRATADEIPYVTAVVTEKFE
                     EVREDFTRIYCVIVVERGSQKKIIIGKGASRLKEIGIQARKEIENLLGHRCHLELFVK
                     VEEDWRNKDQLLDEYGLSGN"
     gene            2195..3058
                     /locus_tag="VFH96_09335"
     CDS             2195..3058
                     /locus_tag="VFH96_09335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET6974214.1"
                     /translation="MSHAKEKPERWYALDVDLEPAAREAVEYALMEAGALGTETRDEQ
                     AGLIRVVAYFADTPDRERVRNELFEALRIYELPSSSVRDMNAREVAQHDWLEEWKQNW
                     QPVEIGRFIIAPPWSNLDEVHDHLIIRIEPGMAFGTGTHETTRLCLAAIQKHFAGGSF
                     LDVGTGTGILAIAAAKLFPEAHIEACDTDETAIEIACENAAANDVAQIEFWVGSVAES
                     TASADLVCANLTADVITQILPALVGVTCGKLILSGILETQVEMVQAGLHDNGIDEFEI
                     EQDGEWVALII"
     gene            3063..3797
                     /locus_tag="VFH96_09340"
     CDS             3063..3797
                     /locus_tag="VFH96_09340"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET6974215.1"
                     /translation="MSRRRFHAPPDAFKQQTITLGTDEARHLREVLRLKTGDEVYVFD
                     GRGREFRCVVETSRRDTAELRIESEIEPAKPESHLQLNLCVALLKGDKFDLVVQKATE
                     LGVTRITPLITRFADIHLRDESDATKRVARWQRIALEAAKQSGRAFVPEISLPVRFES
                     VDVDGLGVMFAERGGEPLENLTRQTSVTSLTALVGSEGGWSDEEIESARARNFHLITL
                     GGRVLRAETAAITVTVLTQHLFGDLK"
     gene            3956..4231
                     /locus_tag="VFH96_09345"
     CDS             3956..4231
                     /locus_tag="VFH96_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974216.1"
                     /translation="MAISKEIAVENARNDLASRLKINASDVKEKSVEDADFPDMALGA
                     AEDDEMSGQMITRGWRIKLEAQGQAFEYRADKNQVRAYKFRGKNYRI"
     gene            4262..>4738
                     /locus_tag="VFH96_09350"
     CDS             4262..>4738
                     /locus_tag="VFH96_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974217.1"
                     /translation="MNRNNIFIHIALALVLILVAAFAGQLNKWRKQWKHPRIVNVEEK
                     KRSTTIEEISINETQSFAAPEILVKFKSGISQEAIERLTAQRHDRVEDRIENAEGWDA
                     IDDLDNADAATLVKEYQQLPEVEYAEPNYEINLDEATAGPLEPILPRDPQFNDQWAL"
BASE COUNT         1193 a         1248 c         1357 g          940 t
ORIGIN      
        1 tatcggcgtc gcgagtgcgc agggccagct taaaccaccc accgaaccag tgatggatcg
       61 cttcctccgt tacgtgaaga tcgatacgca gtcgcaggaa gatcaaacga cgacgcccag
      121 cacgcggaaa caactcaatc tcgcgaacct gctggcgaaa gagttgaccg cgctcggggc
      181 cgagaatgtt cgcgtcagcg agtttggcat cgtctatgcg actgtgccgg gaaatcttcc
      241 cgataactcg aaggtgccgg tcgtcggatt gatcgcgcac atggacactt cgcccgcagt
      301 ctccggtgaa aacgtcaatc cgatcattca caagaattac cagggcggcg acatcacatt
      361 gccgaaagat ccgacacagg taatcacagt cgagaagaat ccggttctca agtcgctcat
      421 cggcgacgac atcatcactg ccgatggcac cactttactc ggttcggacg ataaatcagg
      481 gtgcgctgag atcatgacga tgatcgatat cctgcgccag aatccacagc tcaaacacgg
      541 cacactcgcg atcgctttca cacctgatga agaagtcggc accggtatcg agaagtttga
      601 cgtgaaaggc ttcggcgcga aggtggctta cacagtcgac ggcgaagaac tcggcgagat
      661 cagtgacgag acgtggagcg cacgcacggc aacagttacg tttcagggca agagcacaca
      721 tcccggcacg gccaagggaa tcatgatcaa cgcgatgtat gcgatcgccg attacctcga
      781 tcgttttccg catgatatgt tgcccgagac aaccgagggg cgcgtcggct ttgttcatcc
      841 gtacgcgggt gtgattgacg tcgagacttc gaatctgaag attttgctgc gcgacttcga
      901 catgagtggt ctcgacgcga aagagaagtt gttgcgcgat atggtggccc aaaccgcggc
      961 gaagtttccg caagtgaaga tcacgatcga cgtcaaagag aattacaaga acatgaagga
     1021 agtgctgaag aactatcctg agttgacgac gaacgcgatg gaagccgcta agcgcgccgg
     1081 attgaagccg tttctgaaac cgattcgcgg cgggacagat ggttcgcgtt tgacgttcca
     1141 gggactgccg acgccaaatc tttttaccgg cggcggaaat tttcacggca aactggagtt
     1201 caactcgcgc aagggactgg agaagtcgac ggagacgctc gttaatttag tccagatctt
     1261 tgcggagaag agttagccac agattacacg gatagaagac ggatcttatg actcatgccg
     1321 gctatgttgc gctgatcggt aggcctaatg ccggtaaatc cacactgttg aatcaactcg
     1381 tggggcagaa gattgcggcc gtttccgata agccgcagac cacgagattc aggattcagg
     1441 gtgtaatcac aaagcctgaa ggacagatcg ttttcgtcga tacgccgggc gttcatcaac
     1501 caggttacga gctcaaccgc cggatgatgg ccgctgtgca ggatgcgctc atgggcgttg
     1561 acctcgtctg cctcattcgt gatgtgtcgg tttcaaccgg caacggcgat cgcttcgtgt
     1621 tggacctcgt aaagcgatcc gacaagccgg ccctcctgtt gttgaataag atcgacaagc
     1681 ttgaagaaaa gtctgcgctg ttgccgttga ttgagtggta cagcaaagag cacgactggc
     1741 aagagatcgt tccgatctca gcactcaagc aagatcagac cgatgtgttg atcgaaagct
     1801 gtttgcgtca tctgcccgaa ggtgaaccga tctttgctga agacgagttg acagaccaat
     1861 cgctgcgcgt gctggttgcg gagatcgttc gcgaaaaagt cttgcgcgcg acggcggatg
     1921 aaattcctta cgttacggcc gtcgtcactg agaagttcga agaagttcgc gaagatttca
     1981 cgcgcatcta ctgcgtcatt gtcgtcgaac gaggctcgca aaaaaagatc atcatcggta
     2041 aaggcgcgag ccgtttgaaa gaaatcggca tccaggcgcg caaagagatc gagaacttgc
     2101 tgggtcatcg ctgccacctc gaactgtttg tcaaagtcga agaagactgg cgcaacaaag
     2161 accaattgct cgatgagtat ggattgagtg gaaattgagt cacgcgaaag aaaagccgga
     2221 gcggtggtac gcgctcgacg tcgatctcga gccggcggcg cgcgaagcgg ttgagtatgc
     2281 cttgatggaa gccggcgctt tgggtacaga gactcgcgac gaacaggcgg gactgatccg
     2341 agtcgtcgcg tattttgctg atactcccga tcgcgaacgt gttcgcaacg agctgtttga
     2401 agcgctgcgc atttacgaac tgccgtcgtc ttcggtgcgc gatatgaacg cgcgcgaagt
     2461 agctcaacac gactggctcg aagagtggaa acaaaactgg cagccggttg agattggccg
     2521 cttcattatt gcgccaccgt ggtccaacct ggacgaggtg cacgatcatt tgatcattcg
     2581 catcgagccg ggcatggcct ttggcaccgg cactcatgaa actacgcgcc tttgtctcgc
     2641 tgcaattcag aaacacttcg cgggcggtag ttttctcgac gtcggcactg gcaccggcat
     2701 tctcgcgatt gcggcggcga agctcttccc ggaagctcat atcgaagcgt gcgacaccga
     2761 cgaaactgcg atcgaaattg cgtgtgagaa cgcggcggcg aatgacgtgg cgcagattga
     2821 attttgggtg ggctcagttg cagagtcgac ggcttccgcc gatctcgttt gcgcaaacct
     2881 cacggccgat gtgattactc aaattctgcc ggccctcgtc ggcgtcacgt gtggaaagct
     2941 aatcctctcg ggaatactcg aaacgcaggt cgagatggtc caggctggtc tgcacgacaa
     3001 cggcatcgat gaattcgaga tcgagcagga cggcgagtgg gtagcgctga tcatttaagt
     3061 caatgagtcg tcgaagattc catgccccgc cggatgcttt caaacagcaa accatcacac
     3121 ttggcacgga cgaagcgcgt cacttgcgcg aagtgctgcg actaaagacg ggtgatgagg
     3181 tttacgtttt cgacggccgc ggacgtgaat ttcgttgcgt cgtcgaaacc agccggcgcg
     3241 acacggcgga gttgagaatc gaatccgaaa tcgagcccgc aaaacctgaa tcacacttgc
     3301 aacttaattt gtgcgtcgcg ctgttgaagg gcgataagtt tgacctcgtc gtgcagaagg
     3361 cgacggagct tggtgttacc agaatcactc cgctgattac tcgtttcgcc gacattcatt
     3421 tgcgagacga gtcggacgcg acgaaacgcg tggcgcgttg gcagcgcatc gcactcgaag
     3481 cggcgaagca gtcgggaagg gcttttgtgc cggaaatcag cttgccggtg cgattcgaat
     3541 cagtcgacgt ggacggattg ggcgtgatgt tcgccgaacg tggcggtgaa ccactagaaa
     3601 acttgacgag acaaacctca gtgacatcgc tgacagccct tgtcggttcc gaaggcgggt
     3661 ggtctgacga ggaaatcgaa agcgcccggg cacggaattt ccatctaatc acgctcggcg
     3721 gccgcgttct gcgcgccgaa accgcggcca ttacggtaac cgtgctaaca caacacctct
     3781 ttggagactt gaaatgaaag atcagtttca cgcaaaggcg caaagacaca aaggaagggc
     3841 agacactctg gctcctcttt gccgtctttg cgcctttgcg tgaaaccgat ctttggctca
     3901 gaatacagct aatcgccgga acaaagaact caaaggggtc gttagaaacg tcataatggc
     3961 aatcagcaaa gaaatcgcag tcgaaaacgc ccgcaacgac ctcgccagcc gcctgaaaat
     4021 caacgccagc gacgtcaaag agaaatcagt tgaagacgcc gatttccccg acatggcgct
     4081 cggcgcagcg gaagatgacg agatgagcgg ccagatgatc acgcgtggct ggcgcatcaa
     4141 gctcgaagcc caggggcaag ccttcgaata tcgcgccgac aaaaaccagg tcagagccta
     4201 taaattcagg ggcaagaatt accgaatcta aatcgagccc atccgatata gaggtaccac
     4261 catgaaccgc aacaacattt tcattcacat cgcgctcgcg ctcgtcctca ttctcgtggc
     4321 cgctttcgcc ggtcaactga acaaatggcg gaaacagtgg aaacatccgc ggatcgtgaa
     4381 cgtcgaggag aagaagaggt caacaaccat tgaagagatc tccatcaacg agacgcagag
     4441 ctttgccgcg ccggaaatcc tcgtcaaatt caagagcggc atcagccagg aagccatcga
     4501 gcgcttaaca gcgcagcgtc acgatcgcgt cgaagatcgc atcgagaacg cggaaggctg
     4561 ggatgccatt gacgacctcg acaacgccga cgcagctacg ctcgtgaagg aataccaaca
     4621 gcttccggaa gtcgaatacg cagaaccaaa ttacgaaatc aatcttgacg aagcgaccgc
     4681 cggaccgctc gagccgattc tgccgcgcga cccgcagttc aacgatcagt gggcgtta
//
LOCUS       DASSFQ010000259         9468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2212904, whole genome shotgun sequence.
ACCESSION   DASSFQ010000259 DASSFQ010000000
VERSION     DASSFQ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9468
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212904"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="VFH96_09355"
     CDS             complement(<1..1593)
                     /locus_tag="VFH96_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6974218.1"
                     /translation="MKVVAILGAALRGRPFERDTIRKQRAAREGHPYSCAIFAAILLL
                     FSFPAFAQTKRLVVIKCDGLPYDVVDRYVKQRDPQSGKSALPWIDHIFYQRGARLSNF
                     YVRGMSLSAPSWSLLETGQHLQIKGNVEFDRFTLHSYDYLNFFPLYLAGALGQRIDMR
                     AVEVLDSLGLPMVSDAYPHDERYITYSIFLRNPRYSTLQASLQKKFMKSPKELFDEWT
                     MGLEMRSAVTDQLVHEVITNLNDPKIQYLDLMMEDFDHVAHHNNDEQSHLFVLKRMDA
                     VIGQVWTAIQKSSLADQTAIVLISDHGINTDAKVYSQGYNLVKLLGSKAGGGHHVVTK
                     RRLMLDYAIKGVNPLVPLITTTTPDTYYLKGESTTYPTALLDFDGNERASLHLRDSDV
                     NLLHILLQQLQRGGLSVEARRAATHEFFVVIDRRRADWEKDLKQLQTELGALDRAIVE
                     QRKLWEAQPKKFTKEQQDAGLDDAAKRIYVQLDRWQMERKEYGEYALALQNLLAISSE
                     AFDASKVKIADVIPKMSMGDRNT"
     gene            complement(1598..3637)
                     /locus_tag="VFH96_09360"
     CDS             complement(1598..3637)
                     /locus_tag="VFH96_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974219.1"
                     /translation="MTTQRRHWHLRASFLFALLLVVSPLLPTISKAQGPNVRETEEND
                     PATVLSQIMRLERVEVPGGAELITVFAKLSGLDPKEVSTEKDNFVPLVSILRDTLGDD
                     TPENNRLRYVWPLTYTRPTTKQRLAAAIPFFYTRVGNKEKLTKTPPPALDLASPESEV
                     WDKIFWTALQSILLDPYGTPIKASTSSYRRNTSDYRRSHIIRALSVLALYQAVKGESA
                     FTPAEMQTIQARLFLSEKTFGGLVDDSNLQSYYVKKTTQTRDERGHNWELLRQKAEAE
                     SLYFEPLTMPDGSATHALLWVAKRDLVSKQSSRYSGRFLNIANPWTDKRLLNWKGYVE
                     TRYFDSENQPVDSQTQGAEAVEMIPLGLYGLDNPKIPMLLVDFRDSYNPKKREMSRRV
                     LNDVTRNVLSLSKFGNLPFFLGRTVFDFVTGRRGMDINQPSRLATYSQLKLLLALNTS
                     MEPELRSQVSDQLEKISLNPFDNDLSAEAKIATQQYDALLAYAKDPEGLAEKIERDRR
                     AEMLPLEHGDKARLAFRVLNVLSFGKYVHREELTNDMEDRLDIARRLRYHTNFLQQIA
                     KSTAEVDITWNLEDVKRSLHFIAEHGSEAGSNTAAVTATIFARTKDIETRRACLDSLS
                     RIHNPKAKNELLRISQNNGVDKTLRDLATEYLAKGPRVQPIAATVSGTPAIAGQP"
     gene            complement(3935..5383)
                     /locus_tag="VFH96_09365"
     CDS             complement(3935..5383)
                     /locus_tag="VFH96_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET6974220.1"
                     /translation="MSRTNGAYRVTGFEPAISLTQNGEGVNQGNEIRDGDEFRMADSD
                     IVLHFFPVRDLPAVVPQPPRETHVAPFIEAAAIESAATARRDDAKVFLREFTRELVRE
                     INPSTKLVALAIALALVGGMLYLGFAMYKELQRSRRLIAEQGEQLGQMRDQVSKTNQQ
                     IDNIDESNKQIQYSLSLAVKLRAEYGSGVCLIAGSYYYVEAGTGRPLRYAEAQLNDSG
                     GAVQSGGQDALTPEGNAAIAEYEFVGSGFHVGQGYVLTNRHIAQPWIADDRAQSITVG
                     LKAQPRLRKLIAYFPNIQQAIPLKFKGAANRDDLAVCSLDIPEVPQTIPILPLEADSD
                     AVAVGKSVVMMGYPSGPDRLLALLDDAESRGIQQRYGTPMAQLAYLAETKRIQPLTTQ
                     GSITDLDVRRIVYDARTAEGGSGAPLFGPSGRVIGVNFAVFTENQASNFAVPIRFGIP
                     LLERNGWEAPAKETVDGETATQTTHPEKSGSR"
     gene            5637..6035
                     /locus_tag="VFH96_09370"
     CDS             5637..6035
                     /locus_tag="VFH96_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET6974221.1"
                     /translation="MSGVEFITDCDRLVQLAPIAEAAIQYHVFVCTGKSCSANDSAEV
                     KNAFERELKARGLQYGKVSKGCSPDGTVVLTECGSVGFCAVGAAVMVYPDGVWYAQVR
                     ADDVPEIVEEHIVNGRPVERLALLRIPANK"
     gene            complement(6049..7557)
                     /gene="gcvPB"
                     /locus_tag="VFH96_09375"
     CDS             complement(6049..7557)
                     /gene="gcvPB"
                     /locus_tag="VFH96_09375"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET6974222.1"
                     /translation="MTITKVTTHISPNEQLIFERSQTGRIGYRLPALDVDEVELSIPS
                     EFRREDDLEGMPEVSEVDVIRHFVRMSTWNYSIDLGMYPLGSCTMKYNSRLNEKVARI
                     AGFANLHPLTEEHDAQGALQVIYELQQHLAEITGLPGISLQPAAGAHGEMTGIMIIRA
                     FLDARDGAAASHRRTMLIPDSAHGTNPASAHLSGFSVKTIRSTAEGLTDLDHLRELCA
                     HGEVAGLMLTNPNTLGLFERNIKQICEIVHEAGGLVYMDGANMNALVGVARPGDMGVD
                     VIHLNLHKTFSTPHGGGGPGSGPCCCTKELEPYLPVPRIVKSSDSTYGLDYDQPKSIG
                     RVKAFFGNYGMMLRALSYTLTHGNDGLREATEAAVLNARYVAAGLIEDYDKPFAGAPM
                     HEVVFTDKKQTRKGVHTLDIAKRLIDYGFHPMTIYFPLIVQGAMLIEPTESVGRQELD
                     QFIAAMKSIAVEAVENPELVLNAPHDTRVGRLDEAAAARKPILRWKAADERG"
     gene            complement(7591..8934)
                     /gene="gcvPA"
                     /locus_tag="VFH96_09380"
     CDS             complement(7591..8934)
                     /gene="gcvPA"
                     /locus_tag="VFH96_09380"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET6974223.1"
                     /translation="MRYIPNSPEERSEMLEAMGLGSAEALFDSIPEDLRLKRPLNTPA
                     ALSEIELLDRFEQMGARNAGAGRISFMGGGAYSHYIPTIVDHILSRSEFFTAYTPYQP
                     EISQGTLQVIFEFQTLVCQLTGMEVANASMYDGSTALAEAVLMAERVTKRSKAVASTA
                     THPQYLEVINTYVQHAGIHLELVPFDEKTGQSWQALAGAIDDETAALVVQSPNFFGCI
                     EDLSALADAAHAKGALLIVAITEAMSLGLLKSPGACGADIVVAEGQSFGVPLSFGGPY
                     VGLFATREKYARQIPGRLVGEAYDKKGRRGFVLTLATREQHIRREKATSNICTNEGLI
                     ALAATVYLETMGRRGVQEAAHQCAQKAAYAARAIAKLNGYSLPFTGPYFNEFVVRAPG
                     RAVDLLSKLGKQNNIDGGIALSRFIPDRPNDFLVCVTETNSREQINQLVQALGSL"
     gene            complement(8937..9329)
                     /gene="gcvH"
                     /locus_tag="VFH96_09385"
     CDS             complement(8937..9329)
                     /gene="gcvH"
                     /locus_tag="VFH96_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET6974224.1"
                     /translation="MANVPEDLHYSKDHEWVRVDGDQAIIGITDYAQNSLGDVVYVEL
                     PKAGDEFAANEPFGSVESVKAVSEVFTPIAGTVVQINESLADEPESVNSDPYGEGWMI
                     RLKMSNPGAVDSLLTAAEYEDFTKAESE"
BASE COUNT         1987 a         2572 c         2679 g         2230 t
ORIGIN      
        1 cgtgttgcgg tcgcccatcg acatctttgg aatcacatct gcgattttta ctttcgaagc
       61 atcgaaagct tccgacgaga tcgcgagcag gttttgtagg gcgagcgcgt attcgccgta
      121 ctccttgcgc tccatttgcc agcggtcgag ctgcacgtaa atccgtttcg cagcgtcgtc
      181 gagaccggca tcctgctgtt ctttcgtaaa cttcttcggt tgcgcttccc agagtttgcg
      241 ttgttcgacg atcgcgcgat cgagcgcgcc taattcagtc tgtaattgct tgagatcttt
      301 ttcccagtca gcacgtcgtc gatcgatgac aacgaagaac tcgtgcgtgg cggccctgcg
      361 agcctcgaca ctgagaccgc cgcgctgtag ttgctgcagg aggatgtgca gcaggttgac
      421 gtcgctgtcg cggagatgca gtgacgcgcg ctcgttgccg tcgaagtcga gcagcgcggt
      481 cggataggtt gtgctctcgc ctttcaaata ataggtgtcg ggcgttgttg tcgtaattag
      541 cggcactaac gggttcacgc ccttgatcgc atagtcgagc atcaggcggc gcttggtgac
      601 gacgtggtgt ccaccgccgg ctttgctgcc gagcagcttc acgaggttat aaccctgact
      661 gtagaccttc gcgtcagtat ttatgccgtg atcggaaatc aaaacgatcg cggtctggtc
      721 agccagcgag ctcttctgaa tcgctgtcca tacttgcccg atgacggcgt ccatgcgttt
      781 gagcacgaag aggtgtgatt gctcgtcgtt gttgtggtgt gcgacgtgat cgaagtcctc
      841 catcatcaga tcgaggtact gaatcttcgg atcgttcagg ttcgtgatta cttcgtggac
      901 gagttggtca gtgaccgcgc tccgcatctc gagacccatc gtccactcgt caaacaactc
      961 tttcggcgac ttcatgaact tcttctgtaa actcgcctgc agtgtcgagt agcgcgggtt
     1021 tcgcagaaag atcgagtagg tgatgtaacg ctcgtcatgc gggtaagcgt cagagaccat
     1081 cggcagcccg agggagtcga gcacttcgac cgcgcgcatg tcgatgcgct gtccaagggc
     1141 gcccgcgaga tagagcggga agaagttcag ataatcgtac gagtgcagcg tgaagcgatc
     1201 gaattcaacg ttgcctttta tctgtagatg ctggcctgtt tcgagcaacg accacgaagg
     1261 cgccgagagg ctcatgccgc gcacgtaaaa gttcgagagt cgcgcgccgc gctggtagaa
     1321 aatgtgatcg atccagggaa gggcgctctt gcccgactgc ggatcgcgct gcttgacgta
     1381 tcgatcgacg acgtcgtacg gcagcccgtc gcatttgatt acgacgaggc gtttcgtttg
     1441 tgcgaacgca ggaaacgaga agagaagaag aattgccgca aagatggcac aactgtaggg
     1501 gtggccttcc ctggccgccc gttgcttacg aatcgtgtcc ctttcaaacg ggcggccacg
     1561 gagggccgcc cctagaattg cgacaacctt cataatttca cggctgaccc gctatggctg
     1621 gcgtaccgct gacggttgcg gcaatcggtt ggactctggg gcctttggcg agatattcag
     1681 tcgcaagatc tcgcagcgtc ttatcgacac cattattctg tgagattcgc agcagctcgt
     1741 tcttcgcctt gggattgtga atgcgcgaca aactatcgag acacgcgcgc cgagtctcaa
     1801 tatctttcgt gcgcgcaaag atcgtagcgg ttactgctgc ggtattcgag cccgcttcgg
     1861 aaccgtgctc ggcgatgaag tgcaacgagc gtttcacatc ttccaggttc caggtaatgt
     1921 caacttcagc cgtcgatttt gcgatctgct gcaggaagtt tgtgtggtag cgaagacgac
     1981 gcgcgatatc gagtcgatcc tccatgtcat tcgtgagctc ttcgcggtgc acatacttgc
     2041 caaaactcag cacgttcagc acgcggaagg cgagccgcgc tttgtcaccg tgctcgagcg
     2101 gcaacatctc cgcccggcga tcacgttcaa tcttttcagc caaaccttcc ggatccttcg
     2161 cgtaagcgag cagcgcgtcg tattgctgcg tcgcgatctt tgcttcagcg cttagatcgt
     2221 tgtcaaacgg attgagtgag attttttcga gttgatcact cacttgagaa cgaagctcag
     2281 gctccatcga cgtgtttagc gcgagcagta gttttaactg cgaatacgtg gcgagtcgcg
     2341 aaggctggtt aatgtccatg ccgcgacgac cagtgacaaa gtcgaatacc gtgcgaccaa
     2401 ggaagaacgg caggtttcca aacttcgaca gcgagaggac gtttcgcgtg acgtcattca
     2461 gcacgcgacg cgacatctcg cgcttcttcg gattgtaact gtcgcgaaaa tcgaccagca
     2521 gcatcggaat cttaggatta tcgagaccgt agagaccgag cggaatcatc tcgaccgctt
     2581 cggcgccctg cgtttgactg tcgaccggct gattctcgga atcgaagtag cgagtctcga
     2641 cgtagccctt ccagttcagc agtctcttat ccgtccacgg attagcgata ttcaggaagc
     2701 gaccgctgta acgcgaactc tgcttgctga ctagatcgcg tttcgccacc cagagcagcg
     2761 cgtgcgtagc gctgccgtcc ggcatcgtta atggttcgaa gtagagtgat tctgcttcag
     2821 ctttttgacg cagcaactcc cagttgtgac cgcgttcgtc gcgtgtctgc gtagtcttct
     2881 tgacgtaata actttgcaga ttcgaatcgt cgacgagccc gccgaatgtc ttttcactta
     2941 gaaacagtcg cgcttggatc gtctgcattt cagcgggcgt gaaagctgat tcacctttca
     3001 ctgcctggta cagcgcgagg acggacagcg cacgaatgat gtgcgatcgg cgatagtcgc
     3061 tcgtgttgcg ccggtaagaa ctggtcgacg ccttaatggg cgtgccgtat ggatcgagca
     3121 gtatgctttg cagcgcagtc caaaagatct tgtcccacac ctcgctctca ggcgatgcga
     3181 gatcgagtgc gggcggcggc gtcttcgtca gcttctcttt gttgccgacg cgcgtgtaga
     3241 aaaacggaat cgctgcggcg agacgctgct tggtggtcgg gcgcgtgtat gtcagcggcc
     3301 aaacatagcg cagacgatta ttctcaggcg tatcgtcgcc gagcgtgtcg cgcagaatag
     3361 acacgagggg tacgaagttg tctttctcag tagacacttc ttttggatcg aggcccgaga
     3421 gctttgcgaa gacagtgatc agctccgcgc cgccaggtac ttcgactcgc tcgagccgca
     3481 tgatctgaga aagaaccgtc gccggatcgt tttcctcagt ttcgcggaca tttgggcctt
     3541 gggccttgct gattgtcggg agtagcggtg agacaacgag cagtaaagca aaaagaaagc
     3601 tcgccctcag gtgccagtgc ctacgttggg tagtcatcct gatcagttac tcaatattct
     3661 ggcagatggg gagtttatat tgcacttaaa gcttacaact ctgaacagat cgttgataaa
     3721 actcttggtc gattttcaaa ctgcaatacc tgtgcccgca tgagcacggt tactaaatat
     3781 acattaggtt aaccccgcct ggaccaattt tgtgcttgct gtgtgacacg ttatgcattg
     3841 taacaagcca tttctgattg cctatttcac gaattgtatg acgattttcg ttgagttgcc
     3901 gttaacgagt aactggtaaa agtaccagcg aggatcacct cgatccggat ttctcagggt
     3961 gagtggtttg tgtggcagtt tcgccatcta ctgtctcttt tgcaggcgct tcccaaccgt
     4021 tccgctcgag cagaggaatt ccgaagcgaa tgggaaccgc aaagttcgat gcctggttct
     4081 cagtgaagac tgcaaaatta actccaatca cgcgacccga aggtccaaac agcggcgcgc
     4141 ccgatccacc ttccgcagtc cgtgcgtcgt aaacgattct ccgtacgtcg aggtcggtaa
     4201 tgctgccctg cgtcgtgagg ggttggatgc gttttgtttc ggcgaggtat gcaagctggg
     4261 ccatcggcgt gccgtaacgt tgctgaattc ctctcgactc cgcatcgtcc aacagcgcga
     4321 ggagtcgatc cggaccggaa ggatatccca tcatcacgac agattttccg accgcgaccg
     4381 catccgaatc cgcttccaat ggaagaatcg ggatggtctg cggaacttcg ggaatatcta
     4441 aactacacac ggcaaggtca tctcggtttg cggcgccctt gaacttgagt gggatcgcct
     4501 gttgaatatt tggaaaataa gcaatcagtt ttctcagtcg cggctgcgcc ttgaggccga
     4561 cggtgatgct ttgggcgcga tcgtccgcga tccatggttg agcgatgtga cgattggtaa
     4621 gcacgtaacc ttgtccgacg tgaaatccgc tgccgacaaa ctcatactcc gcaatcgccg
     4681 cgttgccttc aggggttaac gcatcctgcc cgccgctttg aacagcgccg ccactatcgt
     4741 tcaactgcgc ttcggcgtaa cgtagcggcc gtcccgtccc tgcttcgacg tagtaataag
     4801 aacccgcgat caaacaaacg ccgctgccat actcggcacg caactttact gcgagtgaaa
     4861 ggctgtactg aatctgtttg tttgattcat cgatgttgtc gatctgctgg ttggttttgg
     4921 agacctgatc tcgcatctgg ccgagctgtt cgccctgctc ggcgatcaga cgtcgactgc
     4981 gctgtaactc cttgtacatc gcgaatccga gatagagcat tccaccgacg agggcgagag
     5041 cgattgcgag ggcgaccagc ttggtactcg gattgatctc gcgcaccagc tcgcgggtga
     5101 actcgcgcag gaaaaccttg gcgtcgtcgc gtcgtgcagt cgctgctgat tcgattgcgg
     5161 cagcttcgat aaacggcgcg acatgagttt cgcgtggagg ttgtggtacg acggccggaa
     5221 gatcgcgaac ggggaaaaag tgcaacacga tgtccgagtc ggccatgcga aactcgtcgc
     5281 catcgcggat ctcgttcccc tgattaacgc cctcgccgtt ctgcgtgagc gaaatggcgg
     5341 gctcgaaacc cgtcacacga tacgccccgt tcgtccggct caattcaagc aatacggcgt
     5401 tagcggcggt tttcggcagg ctcgaggaac ggagccgcag atcacaggtc tcgcatgcac
     5461 caatgcgaat gttgtcttcg gaaaggattt cggtgtgccg gtcttcgccc gaggagatgt
     5521 ggataaccag gccggttgac ataaagagtt acagaaccaa aacttccgat ccaagtcgag
     5581 tcgcgcgctc gacgtgttca ttctacaaga agcgcaaaga gtacaaaagt gctagcatga
     5641 gcggcgtgga atttattacg gattgcgatc gtttagttca gcttgcgccg attgccgagg
     5701 cggcgatcca gtatcacgtg tttgtgtgta cgggaaagtc ttgttcggca aatgacagtg
     5761 ctgaagtcaa gaatgcgttt gagcgcgaat tgaaagcgcg cgggctgcag tatgggaaag
     5821 tttcgaaggg atgtagtccg gatgggacag tggtgttgac ggagtgtgga tcggttgggt
     5881 tttgtgctgt cggcgcggcc gtgatggtct atccggatgg tgtttggtac gcgcaggttc
     5941 gtgctgacga tgtgccggag atcgttgaag agcacattgt taatgggcga ccggttgaac
     6001 gtctggcgtt gttaaggatc cctgcgaaca aataatctga tccgcgtttt atccgcgttc
     6061 atccgcggct ttccaacgca agattggttt acgcgcggcg gcggcctcat cgagtcgccc
     6121 gacacgagta tcgtgcggtg cattcaaaac cagctcagga ttttcaacag cctcaactgc
     6181 aatcgatttc atcgctgcga tgaattgatc cagctcctgc cggcccactg actccgtagg
     6241 ctcgatcaac atcgcgccct gcacgatcaa tgggaagtag atcgtcatcg gatgaaatcc
     6301 gtaatcgatc aaacgcttcg caatgtcgag cgtgtgtaca cccttacgtg tctgcttttt
     6361 atcagtaaag accacctcgt gcatcggcgc acccgcaaac ggcttgtcgt aatcctcaat
     6421 caatcccgcc gcgacataac gcgcgttgag caccgccgct tccgtggctt cgcgtaaacc
     6481 atcgttgccg tgcgtcagcg tgtaagacag tgcgcgcagc atcatgccgt agttgccgaa
     6541 gaatgctttt acgcgaccga tcgacttcgg ttgatcgtag tcgagcccgt aggttgaatc
     6601 ggaagacttc acgatccgcg gcaccggcag gtacggttcg agctctttcg tacagcaaca
     6661 cggaccagag cctggtccac cgccgccgtg cggagtcgaa aaagtcttgt gcagattcag
     6721 gtgaatgacg tcgacgccca tgtcgccggg acgggcaacg ccgacgagcg cgttcatgtt
     6781 ggcgccgtcc atgtaaacca ggccgcctgc ttcgtgaacg atctcgcaga tctgtttgat
     6841 gttgcgttcg aagagtccga gcgtgtttgg attcgtcagc atcagtccgg cgacctcacc
     6901 atgcgcgcaa agctcgcgca ggtgatcgag atcggtcaga ccttcggcgg tcgagcgaat
     6961 cgttttgaca gagaaaccgg agagatgcgc tgatgctgga ttagttccgt gagctgaatc
     7021 ggggatcagc atcgtacgtc gatgtgacgc agcggcaccg tcgcgcgcgt cgagaaacgc
     7081 cctgatgatc atgatgccgg tcatctcacc gtgcgcgcct gcggctggtt gcagcgagat
     7141 gccgggcaga cctgtgatct cggccagatg ctgttgtagt tcgtagatca cctggagtgc
     7201 gccttgcgcg tcatgttctt cagtcaacgg gtggaggttg gcgaagcccg cgatccgcgc
     7261 gaccttttcg ttgagtcgcg agttgtattt catcgtgcac gagcccaacg ggtacatgcc
     7321 aagatcgatc gagtagttcc aggtggacat gcgaacgaag tgacggatca cgtcgacttc
     7381 gctgacttcc ggcatgcctt cgagatcgtc ttcacgacga aactcgctcg ggatgctcag
     7441 ctcgacttca tcgacgtcga gggccggcag gcgatacccg atgcggcctg tttgcgatcg
     7501 ctcgaagatg agctgctcgt tcggactgat atgagtggtg actttggtaa tcgtcataaa
     7561 aacaaatagg tcatatacgt cctatatgtc ctataaactt cccaacgctt gaactaattg
     7621 attgatctgc tcgcggctgt ttgtctcggt gacgcaaacc agaaaatcat tgggccggtc
     7681 cggtataaac cgcgacagcg cgatcccgcc atctatgttg ttctgtttgc cgagcttgga
     7741 aagaagatcc acggcccgac ctggcgcccg gaccacgaat tcattgaaat acggaccagt
     7801 gaagggaagt gaataaccat tcagcttcgc gatcgcgcgc gccgcgtacg ctgctttctg
     7861 cgcgcactgg tgcgcggctt cctgcacgcc gcgacggccc atcgtctcta aataaacagt
     7921 ggccgcaagc gcgatcagtc cctcattcgt gcagatgttt gacgtcgctt tctcacgtcg
     7981 aatgtgctgc tcgcgcgtgg cgagcgtgag cacgaaacca cggcgtcctt tcttgtcgta
     8041 agcctcaccg acgagccgcc cgggaatctg tcgtgcgtat ttttcgcgag tcgcaaataa
     8101 tccgacgtat ggaccaccaa aagaaagcgg caccccaaac gactgccctt cagccacgac
     8161 gatgtcagcg ccacacgcgc ccggcgattt caaaagtccg agtgacatcg cctcagtgat
     8221 cgcgacgatc agcaatgcgc ccttagcatg cgcggcatcg gcgagtgccg atagatcctc
     8281 gatacagcca aagaagtttg gtgactggac cacgagcgcc gccgtctcgt cgtcgatggc
     8341 gccggccaac gcctgccacg actggcccgt cttttcatcg aacggaacca gctcgagatg
     8401 aatcccggcg tgctgcacgt aggtgtttat tacttccaga tactgcgggt gtgtcgcagt
     8461 cgaagccaca gctttcgatc gctttgttac acgctcggcc atcaacacag cctccgcgag
     8521 tgctgttgag ccgtcgtaca tcgaagcgtt cgccacttcc atacccgtca actggcacac
     8581 gagtgtttga aactcgaaga tgacttgcag tgtgccttgt gaaatctcgg gctggtaagg
     8641 cgtgtaagcg gtgaaaaact cagagcgcga aagaatgtga tcgacgatgg tcggaatgta
     8701 atgagaatac gcgccgccac ccatgaaact gatgcgacca gcgccggcgt tacgcgcgcc
     8761 catctgttcg aagcgatcga gcagctcgat ttctgaaagc gccgcgggcg tgttgagcgg
     8821 tcgctttaaa cgcagatctt cgggaatgga atcaaacagt gcttcggccg atccgagccc
     8881 cattgcttca agcatttcgc tgcgctcttc cggcgagtta ggaatgtagc gcaagattat
     8941 tcgctctcag ctttcgtaaa atcttcgtac tccgcggcgg tcaggaggct atcgactgcg
     9001 cctgggttag acatcttcag ccggatcatc cagccttcgc catacggatc cgagttaaca
     9061 ctttcaggtt cgtcagccag cgattcattg atttgcacca cagtgccggc aatcggagtg
     9121 aagacttcgg agactgcctt cactgactcg acggagccaa acggctcatt cgctgcaaac
     9181 tcatctcccg ccttcggcaa ctcgacgtac actacgtcac ctaatgagtt ttgtgcgtag
     9241 tcagtgatgc cgatgatggc ctgatcgccg tctactctca cccattcatg atctttgctg
     9301 tagtgcaggt cttccggaac gttcgccatg gatcctccat cctctatctt atttgtttcg
     9361 tttgtagaaa ggcgttttgg tgatttgagc gcccaccagt cgcccgcgta catcgatctg
     9421 tatctcctgg cccacattcg catactcgac gggcacgtag gcaagtcc
//
LOCUS       DASSFQ010000260         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_122240, whole genome shotgun sequence.
ACCESSION   DASSFQ010000260 DASSFQ010000000
VERSION     DASSFQ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122240"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFH96_09390"
     CDS             <1..234
                     /locus_tag="VFH96_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974225.1"
                     /translation="AEIHNKLGDAYYYAGRLREAIDSYEKAKDLQPDNAETFYNLAIA
                     YNEAGNQTMAAIEARTLQRLDPKLYEKYLRETM"
     gene            complement(244..1626)
                     /locus_tag="VFH96_09395"
     CDS             complement(244..1626)
                     /locus_tag="VFH96_09395"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6974226.1"
                     /translation="MKEQIKRIFSAWVLISVVIFGGSEASHATQQGKGQAQETVQSVV
                     AKQAALVSEFEVNGLKVLLKRREGSLTVAAGLFIRGGSTNINAQNAGIESLMLSVATE
                     ATTNFPRAKMRTELARMGTVIGSSSNNDYSVLSLATTRMYFDRSWQIFTDVALRPSFT
                     NEDVSLVQKRAVVSLSDDTDSPDVYLQRLQDRVAYAGHPYLNSTSGTVESVAKLTPDD
                     LKRYHSKVMETSRLLLVIVGDLNPAEVQKLVADSLGKLPRGTYKAEEVPALAFPKSTV
                     EITPRELPTNYIQGLFTAPSLTSPDIYAMRVASSMLRDRVFEEVRVKRNLSYAPDAFL
                     RTQAANVGGLYVTAVDANQSIRVMLAEINRLQTQPVDNDDIHAVIAQYLTTYYLGQET
                     NAAQAGELAQYELIGGGWRNSVDFLEKLSAVTPADIQRVAQKYMRNIRFVVLGNPQSV
                     DPTVFTGQAG"
     gene            complement(1623..>2329)
                     /locus_tag="VFH96_09400"
     CDS             complement(1623..>2329)
                     /locus_tag="VFH96_09400"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET6974227.1"
                     /translation="DDVLKMAQEFYGDWAKREKDPFVEFPLVEHPPLPKSEGTVVIQP
                     IQNVIISIGWHGPSIGKDNPATYAADVFSFILRQPNSRFQRNLVDTGLVTGVDLSYYT
                     QRNVGPIMLIAQTTPDKARAAVKALYNEVAHFNDKDYYTDEELESAKALLEADDLYSR
                     EKLSDYTHTLSFWWASTGLEYFRGYLPRLRATSRAEISRYITTYIQGKPHVGLALMSD
                     QAQGQVKIQPQELIGQ"
BASE COUNT          492 a          695 c          636 g          506 t
ORIGIN      
        1 gcggagattc acaacaagct cggtgacgct tactactacg cgggccgttt gcgtgaggcc
       61 atcgattcgt acgagaaagc gaaagacctg caacccgaca acgcagagac cttctacaac
      121 ctggcgatcg cttacaacga agccggaaat caaacgatgg ccgcgatcga agcgcgcacg
      181 ttgcagcgtc tcgatccgaa gctctacgag aaatacctgc gggaaacaat gtagatccgc
      241 agcttaacca gcctggccgg tgaagaccgt cggatccaca ctctgcggat ttcctaaaac
      301 aacaaaccgg atgttgcgca tgtacttttg cgccacgcgc tgaatgtcag ccggagtcac
      361 cgcgctcagc ttctcgagga aatcaaccga gttgcgccag ccacccccga tcaattcata
      421 ctgcgccaat tcacccgcct gcgccgcatt cgtttcctga cccagataat acgtggtcag
      481 gtactgcgcg attacggcgt gaatgtcgtc gttgtctacg ggttgcgttt gcaggcgatt
      541 gatctcagcc agcatcacgc gaatcgattg attcgcatcg accgcggtca cgtaaagtcc
      601 accaacattc gcggcctgcg tgcgcagaaa cgcgtccggc gcatacgaca ggtttcgctt
      661 cacccgaact tcctcaaaca cacgatcgcg caacatcgag ctcgcgacgc gcatcgcgta
      721 gatgtccgga gaagtcaacg acggcgcggt gaacaaaccc tgaatgtagt tcgtgggcaa
      781 ctcacgcggt gtgatctcga cagttgattt cgggaacgcc agcgccggaa cttcttccgc
      841 tttgtaagtg ccgcgcggca gtttgccgag actatccgcc accagctttt gcacctcagc
      901 cggattcaga tcgccgacga tcacgagcag caatcgcgac gtctccatca ctttcgagtg
      961 ataacgcttc agatcatccg gggtgagttt cgcgaccgac tccaccgtgc cgctagtgct
     1021 gttcaggtac ggatgaccgg cgtacgcaac gcgatcctgc aatctctgca gatagacgtc
     1081 agggctgtcc gtgtcgtcgc tgagcgaaac aaccgctcgc ttttgcacga gcgaaacgtc
     1141 ttcattcgtg aacgacgggc gcagcgcgac gtcagtaaag atctgccacg agcgatcgaa
     1201 atacatgcgc gtggtggcca gcgagagcac tgaataatcg ttgtttgagc tactgccgat
     1261 aacggtgccc atgcgcgcta attccgtgcg catcttggcg cggggaaaat tcgtcgtcgc
     1321 ttcggtcgcg acgctgagca tcagcgattc gattcccgcg ttctgcgcgt tgatgttcgt
     1381 cgagccgccg cgaatgaaga gcccggccgc gacggtcaaa cttccctcgc gccgcttgag
     1441 caggactttc agcccgttaa cctcaaactc actgaccagc gccgcctgct tcgcaacgac
     1501 gctttgcaca gtttcctgcg cctgaccttt gccctgttgg gtggcgtgcg aagcttcgct
     1561 gccgccaaaa atgacaaccg agattaaaac ccacgcgcta aatattctct tgatctgttc
     1621 tttcattgac ctatcaactc ttgtggttgg attttgacct gaccttgcgc ttgatccgac
     1681 atcagcgcga gtccgacgtg cggcttacct tgaatgtagg tggtgatgta acggctgatc
     1741 tcggctcgcg acgtggctcg caaccgcggc aggtaaccgc gaaaatactc gagcccggtc
     1801 gacgcccacc agaagctcag agtgtgggtg tagtcgctca gtttttcacg cgaataaaga
     1861 tcgtcggctt cgagcaaggc cttcgcgctt tccagttctt cgtcagtgta gtagtccttg
     1921 tcgttgaagt gagcgacttc gttgtagagc gccttgaccg ccgcgcgtgc tttgtcgggc
     1981 gtggtctgcg caatcagcat gattggccca acgttgcgct gcgtgtagta gctcagatcg
     2041 actccggtca cgagcccggt gtcgacgagg tttcgctgaa accgcgaatt tggctgccgc
     2101 agaatgaatg agaaaacgtc cgccgcgtag gtcgccggat tgtctttgcc gatggacgga
     2161 ccgtgccagc cgatgctgat gatcacgttc tgaatcggct ggataactac cgtgccctcg
     2221 ctcttcggca acggcggatg ttcgacgagc ggaaactcaa caaacgggtc tttctcgcgc
     2281 ttcgcccagt cgccgtagaa ctcctgcgcc atcttcaaca cgtcatccg
//
LOCUS       DASSFQ010000261        15181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_272228, whole genome shotgun sequence.
ACCESSION   DASSFQ010000261 DASSFQ010000000
VERSION     DASSFQ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15181
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272228"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5588)
                     /locus_tag="VFH96_09405"
     CDS             complement(<1..5588)
                     /locus_tag="VFH96_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET6974228.1"
                     /translation="MSAIDIKSEEISPRDKRALLAELLRQRASKPVVAPTSFAQQRLW
                     FLDQLEPGSPLYNVSSAVRLQGPLEVAALERSFEFLVGRHETLRTTFAWQDGKPVQVI
                     APRLSLVIEHCELEEDSLRSVLEEEALRPFDLASGPLLRVKLLRLGAEDHVLSLTMHH
                     IISDGWSTGVLIGELVSLYEAFAKGESPTLPELPIQYADFARWQREWLTGAVLERQLA
                     YWQKQLDDVTTLQLPTDRPRPSIRSHHGARQNFSLPHEISESLIGLARNEGVTLFTLL
                     LAAWQTLLARYTEQTDIVVGSPIANRNRLETESLIGFFVNTLVLRTDLAGNPTFRELL
                     ERVRSTVLDAQEHQDVPFEKIVEELQLERSLSHTPLFQVVFALHTAPAQGVDLREVKL
                     ATLDVQRHTAKFDLMFELFEHNSKINGSLEYSTDLFDDTTITRLLGHYRRLLSTIVEN
                     PEQRILDLPLLSQTEERQLVYDWNEVAPRAEIEQPLHELFEDQVQRTPGNVALVLEDE
                     VLTYRELNARADELVQDLRELGVQPETLVGIFMERSIEMVVAVLGILKAGGAYVPLDI
                     EHPKERLAFMLEDAEPPVLLTQRRLVDRLPELKTVKAVCIDERTSRDGARVQITSGIA
                     PLRPRGESPAYVIYTSGSTGTPKGVVVTHANVVRLFTATSKWFNFNGDDVWTLFHSYA
                     FDFSVWEIWGALLHGGRLVIVPYLVSRQPEAFYQLLLKERVTVLNQTPSAFRQLIQAE
                     QSSWPNASDAPPLALRFVIFGGEALELQSLGPWFDRHGDEQPLLVNMYGITETTVHVT
                     YRPIREQDLATATGSVIGERIADLQIYVLDAARKAVPIGVPGELYVGGAGLARGYLHR
                     PELTAERFVAHPWDENGARLYRTGDRGRYLANGDLEYLGRVDRQVKVRGFRIELGEIE
                     AVLAQHANVSESVVIARDDDGDTRLVAYVVAADDQDLTVTDLRSHMKERLPEYMVPAA
                     FVLLDTLPLTTNGKVDRRALPSPEDARLDLGEAYVAPESEAERILVDVWAEVLKVERV
                     GVCDNYFALGGDSIRSVNVLSLARERGLNFTLQQLFQHQTIRELAQAATSETVSATRT
                     TPFSLLTEADRLKLPENVIDAYPLSRLQSGMLYHFEATSGVSVYHNINSYHLKAQLNE
                     AVFVQAVQQIVARHDVLRTSFDFSSYSEALQLVHKEAELPVEFEDLRHLSPAAQDDVI
                     NSYIESEQQHRFDLSQPTLLRFHIHRTSDDTFQFTLTECHAILDGWSLQSTLAEIFEV
                     YFALLRNESLPDQKPFATSFREFIALEQAALQSEECQRFWQENLSDATVAEISPWYSE
                     STAVERVRARNLVVSIPDEVSEGLHRLAVNAAVPIKSVLLAAHLKVMSMLMGQSDVIT
                     GVVCNGRPEEADGDLIRGLFLNVIPFRQRVRAQTWIELVRETFNAERNLLPFRRYPLP
                     AIQEQMNTPSFFETTFNYVHYHVLDGVLGSGDVQVLEQVEGYEETNFMLNAGFMSHVL
                     SSRVLLRVALDPKKLSEEQSEAIRGYYERVLAAMASTVAGPLETESLLDETEKQNVLV
                     EWNNTKRDFPFDKCFHQLVEAQVERTPEAEAVVFGEQHLSYQDLNRRANRLAHHLHSL
                     GVGPETLVGISVERSIEMVVGILGILKAGGAFVPLDPSYPRERLAFMIEDSGIEALVA
                     QEALLDVLPRYDGKIIDLNRTDMSYRTDNPDPITAPRNAAYVIYTSGSTGTPKGVVLA
                     HRGLCNLVTAQIHAFNVQPSDRVLQFAALSYDASVFELAMALPRGATLVVAPREDLLP
                     GERLISLLREQSITNVTLPPSVLALLPPEECPTLRTIVVAGEACWAELVQRWAVDGQR
                     FFNAYGPTETTVWIRVAECAA"
     gene            complement(5585..8182)
                     /locus_tag="VFH96_09410"
     CDS             complement(5585..8182)
                     /locus_tag="VFH96_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET6974229.1"
                     /translation="MTDFEQDQNYWLQKLAGDLVVTSLALDHHRSARAVTNKERFTFT
                     LDAETTARVRKITNDDETLLFVTFIMACNVCLFKYTGNEDIIVGTTTYYSTSMNRLLA
                     LRTHVTPDQTVKQLLLEIRDTLAAAYAHQKFSFERLIELLNVEHLPHRMPLFDFMVVL
                     ENTDNTDYVKRLKNDLTVSVAVKDGITGAIDYNADLFRRESIEVLARHLQEILRSASE
                     SPDKTIAQLELLSESRRHELVVEYNNSAVEYPKHKTIVQLFEEQVERTPAHIALEFQN
                     ESLTYRELDNRANQLANWLRARGIKPGVPVGIYLEHSPETIVALLGALKAGGYYVPFD
                     TAHPRSRLDFMIADTATPLVLTQDSDWAAIACERATAPISEATPDDLAYVIYTSGSTG
                     EPKGVEITHRSLVNYIWWAKEQYVRDETLAFALYSSLAFDLTVTSIYTPLITGNRIVI
                     YREDGGSSAIEEILKDNKVGVLKLTPSHLALLKIRDNSSSRIQRLIVGGEQFETELAN
                     EVLRSFNGNVEIINEYGPTEATVGCMIHRFNPDEDTQAFVPVGKPAANAQIYVLDDDL
                     KPVAENVLGEMYLAGDGLARGYLKRPALTAEKFLDNPFTPGQRMYRTGDLARWLPHGI
                     IEYAGRKDSQVKYHGHRVELNEIKRALNRHPQIRDSVIVIARDKTGNDAMMAYYVSEQ
                     ELDSAKLHAFLSSSIIEETIPNVFVHLAQLPLTLNGKLDLRALPTLDEVKQRAQHTYV
                     APRNEVEEQLAEIWQQVFGVERIGIHDNFFELGGHSLIATQVIGRVRHKLHTELLVRS
                     VFEAPTIAELAGAIVKLEEQPKLKQTRINKRIDAQRAGHLLSKVDELSESELDTLLAE
                     MLAEEKVSE"
     gene            complement(8202..13877)
                     /locus_tag="VFH96_09415"
     CDS             complement(8202..13877)
                     /locus_tag="VFH96_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET6974230.1"
                     /translation="MENKVIEGFRLSPQQQRLWLLSKQDGAERYCARCAFMLEGDLDV
                     DKLQRAFQDVVARHEILRTRLEMFPGMKLPLQVIDEAVNTAINMELLPLAPNKQLVSF
                     SLPAFCADRPSLRNLMKDLAQSYGGKVEDEPMQYADFAGWQNELLESAEAEVGKLYWR
                     KRDLSESAALKLPYERSSSSSALAHSEEQALKFAGIDEEILLAAMYALLWRLTGQTDL
                     SIGVAHDGRRYEELRSVIGPVAKYLPVACTVEDVPFSQLVQRVHEANAEARQWQDVFT
                     YETDESAFMPFMFEYTETPEPYRIGDLKFSMYNEDVCLDRFKLKLSCNRESDTLKLQF
                     HYDTKYYGATAITYLSNQYCALLNSVVANSDTSIAQLNLLSEAERQRLLIEWNDTTAP
                     YSLDLCLHELFEAQVKRTPGNTAVVYQDTKLSYAELNERANELAQKLRHLGVGPETVV
                     GVMMDRSLEMVVALLGVMKSSGAYLPLDPTYPEERLQFIIDDAKPTVILTTDNTDQSH
                     GSDPDLIRAFDPRHPCYLIYTSGSTGQPKGVMVPHRGICNRLLWMINAFDFRPTDRFL
                     QKTPFTFDAAGWEFFVPLLIGAQVVIAEPGGHQDTAYLVSAIAEHEITVLQLVPSMLQ
                     IVLEEPGFENCHTLRHVFCGGEALPGSLHTRFHSRLKCELHNLYGPTEYSIDAACWPS
                     QPATFERTVPIGRALSNTQLYVLDQNHNPVAVGMTGELFVGGSGLARAYLNRPALTAE
                     RFVPHCFSTEPGARLYRTGDLVRYLPDGNLEFIGRVDHQVKVRGFRIELGEIEAALCR
                     HPQVREAIVVAREDGEKRLVAYIVGELEAATSELRAHLRLSLPEYMIPAAFVVLDKLP
                     LLSNGKVDRKRLPEPQTSRPSLADTYTPPATEAERVLAGIWSEVLRVEQVGIHDNFFE
                     LGGDSILSVQIITRARQAGLHVTAKQVFHRQTIAELAVVASSSAPQRQATQDEVTGPV
                     ELTPAQREFFRQQLAEPAHWNMAFMVGLSDEIEVAALRGAVAAVLRHHDALRNRFERQ
                     ADGEWRQRCVTDFETPFVEVDLSDVADAELRDSIETAAAEWQKSLDLAKGPMFRAVYF
                     KLGPNRGARLLLAMHHLVMDAVSWRIFLEDLQTAYLRNELQPKTTSYQQWALKLQAYA
                     SSTDLQEEVDYWTDERRSRVQRLPVEQEGSNLESTTAWVRNTLSKELTAALLREVPRA
                     YNTQINEVLLAALAGAHQRWTGEQLLVVDAEGHGREALFDDVDLTRTIGWFTSIYPVL
                     LELPDGNDYGQRLKSIKEQVRLVKHGGVGYGVLRYLSPDREIRNQLAAQPQAEISFNY
                     LGRYEQATSEIAEAGLFQAASERPGPLRADAAERLYKLQLVCSVAGGELQISWEYSSA
                     IHHAETVTQLAADYLAELERLIRHCQSPEAGGFTPSDFPEAELTQPQLDELIASLSLT
                     GDPKKEIEAIYPLSHLQEGLLFHSVYDDSSNMYVSHLSCTLGGELEIAAFKQAWERVV
                     ERHDVLRTMFVWEGLSKPLQVVRRQVESPWVEHDWRHYSAVEQQQRLQTYLEKDRGFN
                     LLDAPLMRLTLIRMGDDSYHFTWSHHHLLMDGWSAMRITGEVFALYEAFAGGEDLELP
                     NTLAYRDYIAWLREQDLKEAEGFWRQTLAGFTSPTPIGGLTSSAVKRDCQSLERELPS
                     TLTRDLETLAQEHQLTMNTLLQGAWGLLLSRHSGRDDVLFGGTVSGRPAELPGVEKVV
                     GLFINTLPVRLQVKKGVALVPWLKDLQEQQLDARQYEYAPLVELHEWSELPRHVPLFE
                     SIFIFESFPVEVSFGERSRRLSISDVQFSIEVNYPLAFVIKPRGAALALELIYDRARF
                     DKAYIETMLRHVETLLGSMVAHPGALLDQLQIVEASMAAAPDLSKSFSF"
     gene            complement(13945..>15181)
                     /locus_tag="VFH96_09420"
     CDS             complement(13945..>15181)
                     /locus_tag="VFH96_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET6974231.1"
                     /translation="DGSPAQLIAPAMPFRLPALDLRDLPEAEREIEVRHLLVQESNQP
                     FQLSQAPLLRCLLLQTEDEEHVFVLTVHHIAADGWSMGVFVRELAALYESYLEQRPSM
                     LPELPVQYADFAVWQRNWLRGEVFDRLLDYWQEQLKNAPTRLALPADKPRPAVLTYRG
                     SAQYKSIAPELAQSLADFSNREGVTLYMTLLSGLNTLLHYYTGQSDIVIGTDVANRNR
                     GETESLIGFFVNELALRTDLSGNPTFRQLLSRTREVTLGAYSHQDMPFDRLVDALKIE
                     RSPAHHPLFQVSFVYQNLPRTSIDVSSLTISTLPVENTVARFELVLRAWESGQGIHLM
                     LEYNSDLFMPTTITRLLDLFETLLGIVVQQPEADLNTLVEMLTQADRAQLADKGKALE
                     EIGSKSLKKATRRAVATNI"
BASE COUNT         3263 a         4413 c         4176 g         3329 t
ORIGIN      
        1 gccgcacact ccgccaccct aatccagaca gtagtctcgg ttggtccata cgcgttgaag
       61 aagcgctggc cgtcaaccgc ccaacgctgg accagctcag cccagcatgc ttcaccggca
      121 acgacgatcg tgcgcagcgt aggacattcc tccggcggaa ggagggcgag gactgaaggc
      181 ggcaacgtga cgttcgtgat cgactgctcg cgcagtaacg agatcaaacg ttcgcccgga
      241 agcaggtctt cgcgcggcgc cacaacgagc gtcgcgccgc gcgggagtgc catcgccagc
      301 tcaaacactg aagcgtcgta gctgagcgcc gcgaattgca ggacgcggtc ggaaggttgc
      361 acattgaaag catgtatttg cgcggtcacg agattgcaca gaccgcgatg agcgagcacg
      421 acgcctttcg gcgtgccggt tgagccggac gtgtagatca catacgcggc gttccgaggt
      481 gcagtgatcg gatctggatt atcggtccga taggacatat ccgtcctatt tagatcgatg
      541 atctttccgt cgtaacgagg aagaacgtcg agcagcgcct cctgcgcgac cagtgcctca
      601 atgccggagt cttcgatcat gaaagccagg cgctctcgcg gataacttgg atccagtggc
      661 acgaaagcac cgccggcttt cagaattccg agaatcccca ccaccatctc gattgatcgc
      721 tcgacactga tcccgacgag tgtttccggt ccgactccaa gcgagtgcag gtgatgggcc
      781 aatcgatttg cgcggcggtt cagatcctgg tagctcaggt gctgctcgcc aaagactacg
      841 gcctcagctt ccggcgtccg ctcgacctgg gcttcgacga gctggtggaa acatttatca
      901 aacgggaagt cccgcttcgt gttgttccac tcaaccaaca cgttttgctt ttcagtttca
      961 tcgagaagag actccgtctc tagtgggcca gcaacagtac tcgccatcgc cgccagcacg
     1021 cgctcgtaat atccgcggat tgcttccgac tgttcttcac tcaacttctt cggatcgagc
     1081 gcaacccgca acagcactcg cgagctcaaa acgtgagaca tgaatcccgc attgagcatg
     1141 aagtttgtct cttcgtaacc ttcgacctgt tccagtacct gcacgtcgcc tgaaccgagt
     1201 acgccatcga gcacgtgata gtgaacgtag ttaaaagtcg tttcgaagaa agacggcgta
     1261 ttcatctgtt cctgaatcgc cggcaacgga tagcgtctga atggcagcag gttgcgttcc
     1321 gcgttgaacg tctcgcgcac aagctcgatc cacgtttgcg cacgcacacg ctgtcgaaaa
     1381 ggaatgacgt tgaggaacaa gccccggatc aggtcgccat ccgcttcttc gggccgcccg
     1441 ttgcaaacca cgccggtaat gacgtctgac tggcccatca acatgctcat cactttgagg
     1501 tgcgccgcga gcagcacgct cttgatcggc accgcggcat tcacggccaa acggtgcaga
     1561 ccttctgaaa cctcgtcagg aatgcttaca acgagattcc gcgcccgcac acgctcgacc
     1621 gcggtcgatt ccgaatacca gggcgaaatc tctgcgaccg tggcgtcact caaattctcc
     1681 tgccagaagc gttggcattc ctccgattgt agtgccgctt gttcgagcgc gatgaactca
     1741 cggaacgacg ttgcaaatgg cttctggtcc ggcaacgact cgtttctgag aagggcaaaa
     1801 taaacctcga agatctctgc cagagttgat tgcaaactcc agccgtcgag gattgcgtgg
     1861 cattcagtta acgtgaactg gaacgtgtcg tcgctggtgc ggtggatatg aaagcgaagc
     1921 agagtcggtt gcgacagatc gaaccggtgt tgttgctcgc tttcaatgta ggaattgatc
     1981 acgtcatcct gtgcagccgg agagagatgc cgcaggtctt cgaattcgac cggcagctca
     2041 gcttctttgt ggaccagttg cagcgcttcg gaatatgacg agaaatcgaa cgaagtgcgc
     2101 agcacgtcat gtcgggcgac gatctgctgc accgcctgga caaaaaccgc ttcgttcaac
     2161 tgggctttga ggtgatagct gttgatgttg tgatagacag agacgccgga tgtcgcctcg
     2221 aagtgataaa gcatgcccga ctgcagccgg ctcaatggat acgcatcgat cacgttttcg
     2281 gggagtttta gcctgtcagc ttccgtcaac agactgaatg gcgtggtgcg tgtggcggac
     2341 acggtttcgc tggttgcagc ttgcgcgagc tcgcgaatgg tttgatgttg aaataattgt
     2401 tgcagagtaa aattcaatcc acgctctctc gccagactca acacattcac gctgcgaatc
     2461 gaatcaccac caagcgcaaa gtagttgtcg cataccccaa cgcgctcaac tttcaggact
     2521 tccgcccaaa catcgacgag aattcgttcc gcttcgcttt cgggtgcaac gtaagcctca
     2581 cccagatcga gtcgcgcatc ttccggcgac ggcaacgcgc gtcgatcgac tttgccgttt
     2641 gtggtcaatg gcagcgtgtc gagcaacacg aacgctgcag ggaccatgta ctcgggcagc
     2701 cgctctttca tgtgactgcg caaatcggtc acggtcagat cctggtcatc agccgctacc
     2761 acataagcga cgagtcgcgt atcaccatcg tcgtcgcgcg cgattaccac gctctcactc
     2821 acgttggcgt gctgcgccaa aacagcctcg atttccccca actcgatacg gaagccgcgt
     2881 accttcacct gtcgatcgac tcgacccagg tactcgagat cgccattcgc gagatatcgc
     2941 ccgcgatcac ccgtgcgata cagacgcgcg ccattttcat cccacggatg tgctacaaag
     3001 cgttctgccg taagctccgg ccggtgcaaa taaccacgcg cgagaccagc cccaccgaca
     3061 tacaactcgc cgggcacacc gatcggcaca gccttgcgcg ccgcgtcgag cacgtagatc
     3121 tgcaagtcag cgatacgttc gccaatcaca ctgcccgtgg cggttgcgag atcttgttcg
     3181 cgaattggtc gataggtgac gtggaccgtc gtctccgtga tgccatacat gttgacgagc
     3241 aatggttgct catcgccgtg ccggtcaaac catggtccaa gactctgtag ctcgagcgcc
     3301 tccccgccaa agatgacgaa ccgcaaagcc agcggtggtg cgtctgaagc gttgggccaa
     3361 ctgctctgct cagcctgtat gagttggcga aacgcggaag gagtttgatt aagcaccgtc
     3421 acgcgctctt tgagtagcaa ctggtaaaac gcttccggtt ggcggctcac gagatacggc
     3481 acgataacga gccgtccgcc gtgaagcagc gcgccccaga tttcccaaac cgagaagtcg
     3541 aatgcgtatg aatgaaaaag tgtccagacg tcgtcgccgt tgaagttgaa ccacttgctc
     3601 gtcgcagtga agagacgaac gacattggcg tgtgtgacga ccacgccttt gggagttccg
     3661 gtcgagccgg aagtgtaaat aacgtaagcc ggactttccc cgcgcggacg caaaggcgca
     3721 ataccgctcg ttatttgcac ccttgcgccg tcgcgcgagg tacgctcgtc aatgcaaacc
     3781 gcttttactg tcttcagctc cggcaacctg tcaaccaacc gtcgctgcgt caacagcacc
     3841 ggcggttccg catcttcgag cataaacgcg agccgctctt tcggatgttc gatatcgagc
     3901 ggcacgtatg cgccaccggc tttgaggata ccgagcactg cgaccaccat ctcgatcgag
     3961 cgctccatga aaataccgac gagggtttct ggttgaacgc caagctcgcg aagatcttgc
     4021 accagctcgt cggcacgcgc attcaactca cggtaagtca acacttcatc ttcaagcacc
     4081 agtgcgacgt ttccgggcgt gcgttgcact tgatcctcga ataactcgtg cagtggttgt
     4141 tcgatctcag cgcgtggtgc gacttcgttc cagtcgtaca ccagttgccg ctcttctgtt
     4201 tggctcaaca gtggcagatc gagaattctt tgttcggggt tctcgacgat cgtggatagc
     4261 agtcgcctgt aatgtccgag caggcgcgtg atcgtcgtgt cgtcgaaaag atctgtgctg
     4321 tattccagcg agccgttgat tttgctgttg tgctcgaaca gctcgaacat gaggtcaaac
     4381 ttcgcggtat ggcgctgaac atcgagcgtc gcgagtttta cctcccgcag gtcgacgcct
     4441 tgtgcgggcg cagtgtgcaa cgcaaacacg acctgaaaca gtggtgtgtg gctcaggctt
     4501 cgctcgagct gcaactcttc gacgatcttt tcaaacggca catcctggtg ctcctgcgcg
     4561 tccagcaccg ttgagcgcac acgttcgagc aactcgcgaa aggttggatt gccggctaaa
     4621 tctgtccgca gcacgagtgt gttgacgaaa aatccgatca ggctttcagt ttcaagacgg
     4681 ttgcgattcg cgatgggcga cccgacgacg atgtccgtct gttcggtgta acgggccagc
     4741 agcgtttgcc acgccgcgag cagtaacgtg aagagcgtga caccttcgtt acgagcgagc
     4801 ccgatcagcg actcagaaat ctcgtgcggc aacgagaagt tttgccttgc gccgtgatga
     4861 gatcggatcg acggtcgcgg gcgatccgtt ggtaactgca acgtggtcac atcgtcgagc
     4921 tgtttctgcc agtaagcgag ttgcctttcg agcacagcgc cggtgagcca ctcacgttgc
     4981 cagcgcgcaa agtcggcgta ttggatcggc agctcgggca gcgttgggct ctcacctttt
     5041 gcaaacgctt cgtacaacga caccagctcg ccgatcagca caccagtgga ccagccgtct
     5101 gagatgatgt gatgcatcgt cagcgacaac acgtgatctt cagctccgag acgcaacaac
     5161 ttcacacgca gcaacggccc acttgcgaga tcaaacggcc ttaacgcttc ctcctcgagc
     5221 acactacgta aggaatcctc ttcaagctca cagtgctcaa tcaccagcga aagccgcggc
     5281 gcaatcacct gcaccggttt cccatcctgc caggcaaacg tggtgcgcag ggtctcgtgt
     5341 cgccccacca aaaactcaaa actacgctcc agcgccgcca cctcaagcgg accttgcaga
     5401 cgaaccgcgg agctcacgtt gtacagcgga ctgcccggct ccagttggtc caaaaaccac
     5461 agccgctgct gcgcgaacga agtcggagcc acgaccggct tgctggctcg ctgccttaac
     5521 aactcggcga ggagggcacg cttgtcgcgc ggtgagatct cttcgctttt gatatcgatg
     5581 gcgctcattc gctcaccttc tcttctgcca acatctcggc caacaacgta tccagctcgc
     5641 tctcagaaag ctcatccacc ttcgacaaga gatgacctgc tcgttgtgca tcgatgcgct
     5701 tgttgattct cgtctgcttg agcttcggct gctcctcgag tttcacgatc gcacctgcca
     5761 gctcagcaat cgtcggcgct tcaaacacag agcgcaccag cagctccgtg tgcaacttgt
     5821 gtcgcacgcg cccgatcacc tgcgtcgcga tcagcgaatg cccgccgagc tcgaagaagt
     5881 tgtcatgaat gccgatgcgc tcgactccaa acacctgctg ccagatctcc gccagctgct
     5941 cttcgacctc gttgcgtggc gcgacgtacg tgtgctgcgc tctctgtttc acttcgtcga
     6001 gcgtcgggag ggcgcgcagg tcgagcttgc cgttgagagt gagtgggagt tgggccaagt
     6061 gtacgaagac attgggtatc gtctcttcga tgatgctgct gcttagaaac gcgtgcagct
     6121 tcgcgctatc gagctcctgt tccgacacgt agtaagccat catcgcgtcg ttgcctgttt
     6181 tgtctcgcgc gatcacgatc acactgtcgc gaatttgcgg gtgacgattc agtgcgcgct
     6241 tgatctcgtt caattcgacg cgatgaccgt gatacttgac ctgactgtcc ttgcggccgg
     6301 cgtactcgat gattccgtgc ggcagccagc gcgcaagatc gccggtgcga tacattcgct
     6361 ggcccggcgt gaacggattg tcgaggaatt tttcagccgt gagtgccggt cgcttcagat
     6421 agccgcgcgc taagccgtcg cccgcgagat acatctcgcc gagcacgttt tcagcaacgg
     6481 gcttcagatc atcgtcgagc acgtagattt gtgcgttagc agcaggcttg ccaaccggaa
     6541 cgaaagcctg cgtgtcttcg tcaggattga agcggtgaat catgcagccg acggtcgctt
     6601 cggttggtcc atactcgttg atgatctcga cgtttccatt gaagcttcgc agcacttcat
     6661 tcgccagctc ggtttcaaac tgctcgccgc cgacgatcaa tcgctggata cgtgacgacg
     6721 aattgtcacg aatcttcaag agagcgagat ggctcggcgt cagcttgagc acgccgacct
     6781 tgttgtcttt caggatctct tcaatcgccg agctgccacc gtcttcgcga taaatcacga
     6841 tgcgattgcc ggtgatgagt ggcgtgtaaa tcgatgtcac cgtgagatcg aaagcgagag
     6901 acgagtagag tgcgaacgcg agtgtctcat cgcgcacgta ttgctccttc gcccaccaga
     6961 tgtaattgac gagactgcga tgtgtgatct cgacgccctt cggctcgccc gtcgagcccg
     7021 aggtatagat cacgtaagcc agatcgtcag gcgtggcttc gctgatcgga gccgtagcgc
     7081 gctcgcacgc aatcgccgcc caatcagagt cctgcgtgag aacgagtgga gtagctgtat
     7141 cggcgatcat gaaatcgaga cgtgagcgcg ggtgagccgt gtcgaaaggt acgtagtagc
     7201 cgcctgcttt caacgcaccg aggagtgcga cgatcgtttc cggtgagtgt tcgagataga
     7261 tcccgacggg tacgcctggc ttgataccgc gtgcgcgcag ccagttggcg agctgattcg
     7321 cgcgattatc gagctcgcgg taagtaagac tctcgttttg aaactcgagc gcgatgtgtg
     7381 ccggcgtccg ctcgacttgt tcttcgaaga gctgaacgat cgttttgtgt tttggatact
     7441 cgaccgcgct gttgttgtac tcgacgacga gctcgtgtct gcgcgactcg gagagcagct
     7501 ccagttgcgc gatggtttta tcgggtgact cggaagccga gcgtagtatc tcctgtaagt
     7561 gtcgcgctag aacttcgata ctttcccgcc ggaaaagatc cgcgttgtaa tcgatcgcgc
     7621 ctgtgattcc gtctttgact gctacactca ccgtcaaatc attcttcaat cgctttacat
     7681 aatccgtgtt atccgtgttc tccagcacga ccatgaaatc gaagagcggc atacgatgcg
     7741 gcagatgttc gacgttcagt aattctatga gccgttcaaa agagaacttc tggtgcgcgt
     7801 aggcggcagc gagcgtatct ctgatctcga gcagtagttg tttgactgtc tgatcgggag
     7861 tgacgtgcgt gcgcaaagcg agcagccggt tcatcgaggt gctgtaatac gtggtagtac
     7921 cgacgatgat gtcctcgttg ccggtgtatt taaagaggca aacgttgcac gccatgatga
     7981 acgtgacgaa cagcagcgtt tcgtcgtcgt tagtgatctt gcgaacgcgt gccgtggttt
     8041 cggcgtcaag cgtgaaggtg aaacgttctt tgttcgtgac cgcgcgcgca gaccggtgat
     8101 ggtccagtgc cagactggtc acgacgagat caccagcgag cttctggagc cagtaattct
     8161 ggtcctgttc gaagtcggtc atgttatttt ttgtctggga tttaaaacga gaagctctta
     8221 ctcagatcgg gagccgccgc cattgaggct tcaacgattt gcaactgatc gagcagcgcg
     8281 ccgggatgcg cgaccatgct gccgagcagc gtctcgacgt gacgcagcat cgtctcgatg
     8341 taagccttgt cgaaacgcgc gcggtcgtag atcagctcca gcgccaacgc cgcccctcgg
     8401 ggtttgatca cgaacgcaag cgggtagtta acctcgatac taaactgaac atcgctgatg
     8461 ctcagccgcc ggctgcgctc accaaacgag acttccaccg ggaagctttc aaaaataaag
     8521 atgctctcaa acaacggcac atggcgcggc aactcgctcc actcatgtaa ctccaccagc
     8581 ggcgcgtact catactgtcg cgcatccaac tgctgctcct gcaaatcctt caaccacggc
     8641 accagcgcga cgcctttctt cacctgcaag cgcaccggca aggtgttgat aaagagtcct
     8701 acgacttttt caacaccagg caactccgcc ggacgtcccg agacagtccc accgaagagc
     8761 acatcgtctc gaccactgtg ccggctaagc aacaatcccc acgcaccctg caagagcgta
     8821 ttcatcgtca attgatgctc ctgggccaac gtctccaggt cgcgtgtcaa cgtcgacgga
     8881 agctctcgtt ccagcgactg gcaatctcgt ttgaccgctg aagaggtgag cccaccgatc
     8941 ggcgtcggcg aggtaaaacc ggcgagcgtt tgtcgccaaa agccctctgc ttctttgagg
     9001 tcctgttcgc gcaaccaagc gatgtagtca cgatacgcaa gcgtgtttgg caactctaaa
     9061 tcctcaccac ccgcgaaagc ttcataaagc gcaaagacct cgcccgtgat ccgcatcgcg
     9121 ctccagccat ccatgagcaa gtgatgatga ctccatgtga agtgataact gtcgtcgccc
     9181 attctgatca gagtcaaacg catcagcggc gcatccagca gattaaaacc acgatctttt
     9241 tcgagataag tctgcaaacg ttgctgctgc tcgacggctg agtagtgccg ccagtcgtgt
     9301 tcaacccatg gagattcgac ctgccggcgt acgacttgaa gcggtttgct aagaccttcc
     9361 caaacaaaca tcgtccgcaa cacatcatgg cgttcgacga cgcgctccca tgcctgcttg
     9421 aacgccgcga tctcgagctc tccaccaagc gtacaactca ggtgcgacac atacatgttc
     9481 gaggaatcgt cgtagacgct atgaaagagt aatccctcct gcaagtgcga gagcggatag
     9541 attgcttcaa tctctttctt tggatcgccg gtcagcgaca gcgaggcgat caactcgtca
     9601 agttgtggct gtgttaattc tgcttcggga aaatccgacg gagtaaagcc gccggcttcg
     9661 gggctttggc agtggcggat caatcgctcc aattctgcta aatagtcagc ggcgagttgg
     9721 gtaacggttt cggcgtgatg aatcgcgctg ctgtattccc aactaatctg caactcgcca
     9781 ccggcaacgc tgcaaacgag ctgcaacttg tacagacgct cagccgcatc cgcgcgcagt
     9841 ggtccaggtc gttctgaggc agcctgaaac aagcctgctt ccgcgatctc gctggtcgcc
     9901 tgttcgtacc gtccgaggta attgaaactg atctccgcct gaggctgtgc ggctaactgg
     9961 ttgcgaattt ctctatccgg gctcaggtaa cgcaacacgc cataaccaac gccgccgtgc
    10021 ttcacgagcc ggacctgttc cttgatactc tttagtcgct ggccgtagtc attgccgtcc
    10081 ggcaactcga gtagtaccgg atagatcgac gtgaaccagc caatcgttcg cgtcaaatcg
    10141 acgtcgtcaa acaatgcctc gcggccgtgt ccttcagcgt ctacgacgag tagttgctca
    10201 ccggtccagc gttgatgcgc accagccagg gcggccagca gcacttcgtt gatctgtgtg
    10261 ttgtatgcgc gcgggacttc tcgtagcagt gccgcagtta gttctttcga gagcgtgtta
    10321 cggacccagg cggtggtgct ttcgaggttg cttccttcct gttccacagg caatcgctgc
    10381 acgcgcgagc ggcgctcgtc cgtccaataa tcaacttctt cctgcaagtc tgtactcgac
    10441 gcataagcct gcagtttgag cgcccattgc tgatacgaag tcgtcttcgg ctgcaactcg
    10501 tttcgcaggt aagcagtttg caagtcttcc agaaagatgc gccatgaaac cgcgtccatc
    10561 acgaggtgat gcatcgcgag caacagccgc gcgccacgat tcgggcccag cttgaaatac
    10621 accgctcgga acattggccc cttcgcgaga tccaaactct tctgccactc tgccgccgct
    10681 gtctcgatcg agtcgcgtag ttctgcgtct gcaacatcac tgagatctac ttctacaaat
    10741 ggcgtttcaa aatcggtaac acaccgctgc cgccattcac catccgcctg tcgttcaaaa
    10801 cgattgcgca aagcatcgtg atgacgaagc actgctgcaa ccgcaccgcg taaggcagca
    10861 acctcgatct cgtctgacag accgaccatg aacgccatgt tccagtgcgc cggttccgcg
    10921 agttgttgcc tgaagaactc tcgctgcgcc ggcgtcaatt cgactggtcc ggtcacttcg
    10981 tcctgtgttg cctggcgctg cggcgcactg ctactcgcca ctactgccag ctccgcaatc
    11041 gtctggcgat gaaaaacctg cttggcggtg acatgcaacc cggcctggcg cgcgcgcgtg
    11101 atgatctgca cgctgagaat tgaatcacca ccgagctcga agaagttgtc gtgtatgcca
    11161 acctgctcca cgcgcagcac ttcactccag ataccggcca gcactcgctc tgcttcggtt
    11221 gccggcggtg tataagtatc ggcgagtgag ggacggctcg tctgcggctc cggcagtcgt
    11281 ttgcgatcca ccttgccgtt actcaacaac ggcaacttgt cgagcacgac gaaagcagcg
    11341 gggatcatgt attccggcaa gctcagtctc agatgagcgc gcaactcact ggtcgcggct
    11401 tccaactcgc caacgatgta agcgacgagc cgcttttcac catcctcgcg cgccactacg
    11461 atcgcttcgc gcacttgcgg atggcgacac aacgctgcct caatctcacc aagctcgatg
    11521 cgaaagccac gcaccttcac ctgatggtcc acacgtccga tgaactccag atttccatca
    11581 ggcaggtaac gcacgagatc gcctgtgcga taaaggcgcg caccgggttc ggtcgagaag
    11641 caatgcggca cgaatcgttc ggctgtcaac gcgggccgat tcagatatgc gcgcgcgagc
    11701 ccgctgccgc cgacgaatag ctcgccggtc atgccaaccg caacagggtt gtggttctga
    11761 tcgagtacgt acaattgcgt gttgctgagt gcgcggccga tcggcactgt gcgctcgaat
    11821 gtcgcgggct gtgaaggcca gcaggccgca tcgatcgaat actctgtggg accgtaaaga
    11881 ttgtgcagct cacacttgag acggctgtgg aagcgggtgt gcaaactgcc gggcagcgct
    11941 tcgccgccgc aaaaaacatg gcgcagtgtg tgacaattct caaagcctgg ttcttccagc
    12001 actatctgca acatcgaggg gacgagttgc agcaccgtga tctcatgctc tgcgatcgca
    12061 ctgacgagat aagcggtgtc ctggtgtccg ccgggctcag cgatgacgac ttgcgcgccg
    12121 atcagcagcg gcacgaagaa ctcccaaccg gcagcgtcga aagtaaatgg cgtcttctgt
    12181 aagaagcgat cggtgggccg gaagtcgaaa gcattgatca tccacaacag gcgattgcag
    12241 atgccgcggt gtgggaccat cacgcctttt ggctgaccgg tcgagcccga tgtgtaaatc
    12301 aggtaacacg gatgccgcgg atcaaaagcg cggattaaat ccggatctga tccgtggctc
    12361 tgatcggtgt tatctgtggt taagatcacc gtcggtttcg cgtcgtcgat gatgaactgg
    12421 agccgctctt cgggataagt gggatccaat ggcagatacg cgccactgga tttcattact
    12481 cctaacaacg cgaccaccat ctcgagagag cggtccatca ttacgccgac gactgtctct
    12541 ggcccgacgc cgaggtggcg cagtttctgt gcgagctcgt tagcgcgttc gttcaactcg
    12601 gcgtagctga gtttcgtgtc ctgatagacg acggcggtgt tgcctggagt gcgtttgacc
    12661 tgcgcttcga atagttcgtg gaggcaaaga tcgagtgagt aaggcgccgt tgtgtcgttc
    12721 cactcaatta aaagacgctg tcgctcggct tcgctgagga gatttaattg cgcgatgctg
    12781 gtgtcggagt tggcaacgac gctgttcagt agagcgcaat attggttgga cagatacgtg
    12841 atcgcagttg cgccgtagta cttcgtatcg tagtgaaact gcaacttcaa cgtatcggat
    12901 tcacgattgc acgacagctt gagcttgaat cgatcgagac agacgtcctc gttgtacatt
    12961 gagaacttta gatcgccaat gcggtaaggc tccggggttt cggtgtactc gaacatgaat
    13021 ggcatgaagg cggattcgtc agtctcgtaa gtaaacacat cctgccattg tcgggcctct
    13081 gcgttagcct catgcactcg ctgcacgagc tgcgaaaacg ggacatcttc caccgtgcaa
    13141 gcaacaggta aatacttcgc aaccggtccg atgacactgc ggagctcttc gtacctgcga
    13201 ccatcatgcg cgacgccaat cgacaaatcc gtttgacccg tgagccgcca cagcaacgca
    13261 tacattgctg caagcaggat ctcttcgtcg attccggcaa atttaagtgc ctgttcctcg
    13321 gaatgagcca gtgcgctgga agaggaagaa cgttcgtacg ggagcttcag cgcggctgat
    13381 tctgacaagt cacgcttgcg ccagtaaagc tttccgactt cagcctctgc ggattcaagc
    13441 agttcattct gccagccggc gaagtccgca tactgcatcg gctcgtcttc aaccttccct
    13501 ccgtacgact gggccaggtc tttcatcaga ttgcgcaagc tcggacggtc tgcgcaaaac
    13561 gccggcagac tgaaagacac cagttgcttg tttggcgcga gcggtaagag ctccatgttt
    13621 atcgcagtgt tgacagcctc atcgatgacc tggagcggca acttcatccc cggaaacatc
    13681 tccaaacgcg ttcgcaaaat ctcgtgacgc gccacgacgt cctgaaacgc gcgctgcaac
    13741 ttatccacat cgagatcgcc ctcgagcata aacgcgcaac gagcacagta tctttccgca
    13801 ccgtcctgct tactcaacag ccagagacgt tgttgctgcg gcgatagccg aaagccttcg
    13861 atgactttgt tttccatctt gataattctg taaatcctga aatcctgtaa acaatttctg
    13921 acaggattac aggattggca ggattcaaat atttgtggcg acggcgcggc gtgttgcttt
    13981 cttgagactc tttgatccga tctcttcgag cgcttttccc ttgtcggcga gttgcgcgcg
    14041 gtcggcctga gtgagcattt cgacgagcgt gttgaggtcc gcttcgggtt gttggaccac
    14101 gatgccgagc agtgtctcga atagatccag caggcgcgtg atcgtcgtcg gcatgaagag
    14161 atcggagttg tactcgagca tcagatggat tccctgcccg ctctcccagg cacgcaacac
    14221 gagctcaaaa cgtgccaccg tattctcaac cggcagtgtg ctgatcgtga gtgagctgac
    14281 gtcaatcgag gtgcgcggca agttctgata gacgaacgac acctgaaaca gcggatgatg
    14341 agcgggtgag cgctcgatct tgagcgcatc cacgagcctg tcaaacggca tgtcctgatg
    14401 actgtaagca ccgagtgtga cttcgcgcgt gcggctcaat agctgccgga acgttggatt
    14461 gccggaaagg tccgtgcgca gcgccagctc attaacaaag aagccgatga gcgattcagt
    14521 ctcgccgcga ttgcgattcg ccacatcggt tccgatgacg atgtcgctct gcccggtgta
    14581 gtaatggagc agcgtgttca aaccagagag cagcgtcatg tacaacgtca ctccctcgcg
    14641 attactgaaa tccgctaaag actgcgccag ctccggcgca atcgatttat attgcgccga
    14701 gccccggtaa gtcagaaccg ccggtcgggg cttatcagcc ggtagcgcca aacgtgtggg
    14761 cgcattcttc aattgctctt gccagtaatc aagtaaccta tcgaacactt caccacgcag
    14821 ccagttgcgt tgccacaccg caaagtcagc atattgcacc ggcagctcgg gaagcattga
    14881 cggcctttgc tccaggtacg attcatacaa cgccgccagc tcgcgcacga acacacccat
    14941 ggaccagcca tccgccgcaa tgtgatgcac cgtgagaacg aagacgtgtt cttcatcctc
    15001 ggtttgcagc agcagacagc gaagcaaagg tgcttgtgag agttgaaaag gctggttcga
    15061 ttcttggacc agcaaatgtc tcacttcgat ctcgcgctct gcttcgggca gatcgcgcag
    15121 atcgagtgcc ggtaacctga atggcattgc aggggcaatc aactgtgccg gactgccgtc
    15181 g
//
LOCUS       DASSFQ010000262         4911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1902826, whole genome shotgun sequence.
ACCESSION   DASSFQ010000262 DASSFQ010000000
VERSION     DASSFQ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902826"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /gene="trpC"
                     /locus_tag="VFH96_09425"
     CDS             complement(<1..693)
                     /gene="trpC"
                     /locus_tag="VFH96_09425"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET6974232.1"
                     /translation="MDILSEIIAKKRERLLVEKNAVPIDEMRRRALEVRSSATAYALT
                     TALRRDEINVIAEFKRRSPSKGIIRPDADLVQIVKSYEAGGAAAISVLTEEDYFSGSL
                     EDLRTVKATVKLPVLRKDFVFEEYQVYESAAAGADAILLIVAALDSDLLGQLRSLADD
                     LGLDALVEVHTSDEMKRAVACGAKLIGVNNRDLRTFEVSLDSSLSLAHEAPAEALLVS
                     ESGLRERDDLRRL"
     gene            complement(699..1727)
                     /gene="trpD"
                     /locus_tag="VFH96_09430"
     CDS             complement(699..1727)
                     /gene="trpD"
                     /locus_tag="VFH96_09430"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET6974233.1"
                     /translation="MATHRLTDFLLRLVRREDLSRDEAFELLEALLDAEATDAQISGT
                     LVALAAKGETIDELAGMAEGLRSRATRVISQHRCFIDTAGTGSSRAKTFNISTAAAFV
                     IAGAGLPVAKHGNRAASSKSGSADLLTALGVNVSAIPLVSENALNEIGICFMFAPLYH
                     GATARVAGIRRQLGIHTTFNLLGPLSNPAGAPRQIIGVWHKELAEKLAHALSELGTEH
                     AWVVHGEDGLDEITLAGKTHVAEAQDGQVKTFAIEPGDFGFETGSLDHLRGGDADANA
                     AIVNAVLDGSRHDEARALVIVNAAAALFLGGAAGDLSVGAKLAAESIDSGAAQHKLDL
                     LIEATSGP"
     gene            complement(1739..2305)
                     /locus_tag="VFH96_09435"
     CDS             complement(1739..2305)
                     /locus_tag="VFH96_09435"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET6974234.1"
                     /translation="MLLVIDNYDSFTYNLVQYLGELGQTVAVRRNNRVTLDEIENELR
                     PERIVISPGPGTPNDAGITLEVIERFSGKIPLLGVCLGHQAIGQAFGGRVIRAPELMH
                     GKASEVRHDGKTIFAGIDDPLLAGRYHSLIVEKESLPSCLEVSAHTADDIIMGLRHRE
                     LKVEGVQFHPESILTSEGKKLLANFLTL"
     gene            complement(2314..2961)
                     /locus_tag="VFH96_09440"
     CDS             complement(2314..2961)
                     /locus_tag="VFH96_09440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6974235.1"
                     /translation="MNKIALVVFSLLLLTEVRAQSNAPIEAPKKANARTTAEAPAPAE
                     PFDGASVEKMGAQCVTLETELGAIEIAMMPEVAPESVRNFLNLSATGALDTTTFSRVV
                     KGFVIQGGNLQTSEKWGMELARRMTHKLPDEPGLVKHVRGIVSMARNDEPNSATTHFF
                     ILVGTGQHLDSKFAAFGTVTKGMEVVDAINQAPAENEKPEKPVKINRAVVSPCKK"
     gene            complement(2998..4509)
                     /locus_tag="VFH96_09445"
     CDS             complement(2998..4509)
                     /locus_tag="VFH96_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET6974236.1"
                     /translation="MLDLQPSTFEAFEREARQGNVVPVVRSVLADLHTPVGAFMRIAG
                     DSPYAFLLESVEGGERIARYSFLGSEPEMIVRGKGMQTVVERDGKTETFPTFATEWVK
                     DYFRGRSLARRPGLVPFAGGAVGYLGYDAAQWFEPALRDDRTQPLEVPIDAVWMFFRN
                     VIAFDRVKQRLEIVSIVFTEEAEGSHERLRELYDQAVARTQAVEEKIFDSTALPTSAP
                     PLNENAENISLQSNFPRRAFEESVRTVKDHIAAGDCYQVVLSQRFSAPFNGNPLQIYR
                     ALRAINPSPYMFFLRIGDETVVGASPEMLVRCHGQRLDYRPIAGTRKRGATETEDWML
                     SEDLKSDEKEVAEHMMLVDLGRNDLGRVSDYGSVKVDELMRVERYSHVQHLETSLRSR
                     LRDGLDRFDALASCFPAGTVTGAPKIRAMEIIRGLEPDRRGIYAGSVLYMDYADNLDS
                     CIAIRTIVLRNGEALVQAGAGIVADSIPEHEYAETVNKARAMFRAIEMAGRGL"
     gene            complement(4520..>4911)
                     /locus_tag="VFH96_09450"
     CDS             complement(4520..>4911)
                     /locus_tag="VFH96_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6974237.1"
                     /translation="FAQLKEHRGQILNISATLHYLGTPMQLHVSAAKAGVDALTRNLA
                     VEWGRYGIRVNAIAPGPIEDTEGMTRLVPQPIKDRLQKQIPLGRFGRIADIEKAAVFI
                     CSDAANFINGVVLVVDGGHWLTGTRLG"
BASE COUNT         1108 a         1533 c         1242 g         1028 t
ORIGIN      
        1 cagacgtcgt agatcgtcgc gttcgcgcag accactctca ctgacgagta aagcttccgc
       61 tggtgcttcg tgggctaacg aaagcgacga gtcgagcgat acttcgaagg ttcgcaagtc
      121 acgattgttg acgccgatga gtttcgcgcc gcacgccacc gctcgcttca tttcgtcgct
      181 cgtgtgaacc tccacgagag cgtcgaggcc gagatcatca gcgagacttc ttaattgccc
      241 gagcagatcg ctatcaagcg ccgcgacgat cagcagaatc gcatctgcac ccgccgctgc
      301 cgattcgtac acctggtact cttcaaaaac aaaatccttg cgcaacaccg gcagcttcac
      361 agttgctttt actgttcgca aatcttcgag cgatccggaa aagtaatcct cctcagtcag
      421 cacagaaatc gctgctgcgc ctccggcctc atagcttttc acgatctgaa ccagatcggc
      481 atcgggccgg ataattccct tcgacggcga acggcgtttg aactcggcaa tgacgttgat
      541 ttcatcgcga cgcagagctg tcgtcaatgc gtaagcagta gcactgcttc gcacttcaag
      601 cgcacgacga cgcatctcgt caatcggcac cgcattcttc tcgacgagca gacgctctcg
      661 tttcttcgca ataatttccg acagtatgtc catcaaactt acggcccgct ggtcgcttca
      721 atcagcagat ccaacttatg ctgagcggcg ccagaatcga tcgattccgc cgccagcttc
      781 gcccccacgc tcaaatcacc cgcagccccg cccagaaaga gagcggcggc cgcattcacg
      841 atcaccagcg ctcgcgcttc gtcatgtcgc gatccatcca acacagcgtt cacgatcgca
      901 gcattcgcgt cagcatctcc accgcgcaaa tggtcgagtg atcctgtttc aaaaccaaaa
      961 tctccaggtt caattgcaaa cgtcttcacc tgcccgtcct gcgcttctgc aacatgcgtc
     1021 tttcccgcga gtgtgatctc gtcgagcccg tcctcgccat ggaccaccca cgcgtgctca
     1081 gtcccaagct ctgaaagcgc atgggccaac ttctccgcca actctttgtg ccacacaccg
     1141 atgatctggc gcggcgcacc ggccggatta ctcaacggcc ctagcagatt gaaagtcgtg
     1201 tgaataccga gttgccgccg aatccctgca acacgagccg tcgcgccatg atagagcggc
     1261 gcaaacataa aacagattcc gatctcgttg agcgcattct cggaaaccag agggatcgcg
     1321 gaaacattaa cgcccaacgc cgtcagcaaa tcagcacttc ccgacttgct gctggcggcg
     1381 cgattaccgt gcttcgccac cggcaacccg gcgccggcaa taacgaaagc cgctgccgtc
     1441 gaaatgttga atgtcttagc gcgactcgat ccggttccgg cggtgtcgat aaaacatcga
     1501 tgctgcgaaa tgacgcgtgt ggctcgcgag cgcagtcctt cagccatacc ggcgagttcg
     1561 tcaatcgttt cacctttcgc tgcgagagcg acgagcgtgc cggaaatctg agcatccgtc
     1621 gcttcggcat caagcaaggc ctccaacaac tcgaaagcct cgtcgcgcga cagatcttca
     1681 cgacgaacga ggcgcaaaag aaaatcagtt aagcgatgcg tggccatgaa ggaaggagtt
     1741 aaagcgtcaa aaagttggcg agcaatttct tgccctcaga agtcaggatc gattcgggat
     1801 gaaattgcac accttcgact ttcaactccc gatggcgcaa gcccatgatg atgtcgtcgg
     1861 cagtgtgcgc agaaacttcc agacacgagg gcagtgattc cttctcgacg atgagcgagt
     1921 gatagcggcc agccaacaac ggatcgtcga ttccggcaaa gatcgtcttg ccatcgtgcc
     1981 gcacctcaga cgctttcccg tgcatcaact caggcgcgcg aatcactcgg ccgccaaacg
     2041 cctgaccaat tgcctgatga ccaaggcaca cgccgagcaa agggatcttt ccggagaaac
     2101 gttcgatgac ctcgagcgtg ataccggcat cgttgggagt accaggaccg ggtgaaatca
     2161 caatccgttc cggtcgcaac tcgttctcga tctcatcgag cgtgacgcga ttgttgcgcc
     2221 gcactgcaac tgtttgccct aactcgccga gatactgcac gaggttgtag gtgaatgagt
     2281 cgtagttgtc gattaccagc aacataacgc gaattatttt ttacacggac tgaccacggc
     2341 gcgattgatc tttaccggtt tctcgggttt ttcattttct gcgggcgcct ggttgattgc
     2401 gtccaccact tccatccctt tggtcaccgt gccaaacgcg gcaaacttgc tatcgaggtg
     2461 ttgtcccgtt ccgacgagaa tgaagaaatg cgtggtggcg ctgttaggct cgtcattgcg
     2521 cgccatcgat acgattccgc gcacatgttt gacgagaccc ggctcatccg gaagtttgtg
     2581 cgtcattcgt cgcgccagtt ccatgcccca cttctcgctg gtctgaagat ttcctccctg
     2641 gatcacaaac cctttgacga cgcgactaaa cgtcgtcgta tccaacgcgc ccgtcgccga
     2701 aagattgaga aagtttcgca ctgactcagg cgcaacttcg ggcatcatcg cgatctcgat
     2761 cgcacccaac tccgtttcca gggtcacaca ttgcgcgccc atcttctcaa ccgacgcgcc
     2821 atcgaacggc tctgccggag cgggcgcctc tgccgtcgta cgagcgttcg ccttcttcgg
     2881 cgcttcaatt ggcgcattcg actgcgccct gacttccgtt agcaacaaca aactaaatac
     2941 aacaagtgca atcttattca tcatgaaaga tccgtgaaaa tcctgcctaa cgatccgtca
     3001 caatccgcgt cccgccatct cgatcgcacg aaacatcgcg cgcgccttgt tcactgtctc
     3061 cgcatactca tgctcgggga tcgagtcggc aacaattccc gcgccagcct ggacgagcgc
     3121 ctcaccattc cgcaagacaa tcgtccggat tgcgatgcac gaatcgagat tgtcagcgta
     3181 atccatgtac agaaccgagc ccgcgtagat cccgcgccga tccggttcga gcccgcgaat
     3241 gatctccatc gcgcgaatct tcggcgcgcc cgtcacggtc ccggccggaa aacacgacgc
     3301 gagggcatcg aagcgatcga gtccatcgcg caaccgcgat ctcaaactcg tctccagatg
     3361 ctgcacatgc gaatagcgct cgacccgcat caactcgtca actttaacag acccataatc
     3421 cgaaacgcgc ccgagatcgt tgcgaccgag atcgacgagc atcatgtgct ccgcgacctc
     3481 tttctcatcc gacttcaagt cctcactcaa catccagtct tccgtttcag ttgcgccacg
     3541 ttttctggtg cccgcaatcg gccggtagtc gagtcgctgc ccgtgacagc gaaccagcat
     3601 ctctggcgac gcgccgacca cggtttcgtc gccgattcgc agaaaaaaca tgtacggtga
     3661 aggattgatc gcacgcaacg ctcgatagat ctggagcggg ttgccgttga acggagccga
     3721 gaatcgttgc gaaagcacta cttgataaca gtcgccggcg gctatgtgat cctttaccgt
     3781 gcgcacgctc tcttcgaaag cacgtcgtgg aaaatttgac tgtaacgaaa tattttccgc
     3841 gttctcatta agcggcgggg cgctcgtcgg taacgctgtc gagtcgaaga tcttctcttc
     3901 tactgcctgc gtgcgtgcga cggcctgatc gtaaagctcc cgcaaacgct cgtgactgcc
     3961 ttctgcttct tcagtgaaga cgatcgatac gatctccaac cgctgcttga cgcgatcgaa
     4021 ggcgatcacg ttacgaaaaa acatccagac tgcgtcgatc ggcacctcga gtggctgcgt
     4081 ccgatcgtca cgcaacgccg gttcaaacca ctgcgccgca tcgtaaccga gatagccgac
     4141 agcgccgccc gcaaaaggca cgagcccagg tcgacgcgcg agtgaacgac cgcggaaata
     4201 atctttgacc cactcggttg cgaaagtcgg aaacgtctcg gtcttaccgt cacgctcgac
     4261 gacagtctgc attcctttgc cgcgcacgat catttccggt tcggaaccga ggaatgaata
     4321 gcgagcgatc cgctcgccac cttcaactga ttcaagcaga aacgcgtacg gcgaatcacc
     4381 agcgatacgc atgaacgcgc cgaccggcgt gtgcaagtca gccagcaccg agcgcacgac
     4441 gggcacgaca ttgccctgac gcgcctcgcg ttcaaatgcc tcaaacgttg atggctgcag
     4501 atcgagcatt gcgttgtttc taaccgagtc gagtgccggt caaccagtga ccaccatcga
     4561 caaccaacac cacgccgtta ataaaattcg ccgcgtcaga acagataaac acggccgctt
     4621 tctcaatgtc ggcaatgcgt ccgaaacgtc cgagcggaat ctgcttttgc aaacgatcct
     4681 tgatcggttg cggcaccaga cgcgtcatgc cttcggtatc ttcgattgga ccaggcgcga
     4741 ttgcgttcac gcgtatgccg tagcgacccc attcgaccgc aagattacga gtcaaggcat
     4801 cgacacctgc tttcgcggca gagacgtgaa gctgcatggg cgtgccgagg taatgaagcg
     4861 tcgcgctgat gttcaagatc tgtccacgat gctctttgag ttgcgcgaac g
//
LOCUS       DASSFQ010000263         7465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2215443, whole genome shotgun sequence.
ACCESSION   DASSFQ010000263 DASSFQ010000000
VERSION     DASSFQ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215443"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5274)
                     /locus_tag="VFH96_09455"
     CDS             complement(<1..5274)
                     /locus_tag="VFH96_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET6974238.1"
                     /translation="MNDRNVDHPLSFAQERLWLAQAIEPESYRYNEVIVHRVVGPLRP
                     AVFAEALVQLAERHEILRTTYSLRAEGPCQTAQPASILALNVVDLSAFDASVALEILR
                     RELERVRENPFRLDREPPVRAVLFQCSPDQSFLAIGVHHIACDRTSAMIFIDELCLIY
                     EALRDGKEPALPPVAAQYSAFAAWERRSFTDERLDREAEYWRSALEDARPPLLPFRKR
                     SDQNTLAAGAHTLTLDPQFVKSLSQTARKSGTTIANVLLTGLACLLSRYTSQSNITIA
                     LPLAARHKPQLQKLIGLFVNTVVMPVDVPGKSSFAELLSRVSRRAFEVYARGDVPFSR
                     VAERHGVAIEPLLEVAWTFLGAELPASRMSGGVRFEPEDGVLKDKAVFPLEFLVVESQ
                     GSIQIRLVYAKDLFDGEPLTRFLTNYENLLRAGASNPGQPISRVLLDQAEVDSLFALG
                     TGPEQPLPDKDFVQLFRGSVGNAPHAAAVTFGDQTITYSELDAWSNRIANIIREASLE
                     TEDAVAVMLERSPEVVVSLLACLKAGVSYLPISRAYPRERVQEILSDASVKLVLTVRG
                     FADDAASVQVVCLDEISAGNDGFEVETPVPRNRLAYIIYTSGSTGRPKGVMVQRGGML
                     NHLLAKVKALELGSTDVVAFNAPISFDISIWQMLTPLLTGACIRIVSEAESNDPVLLI
                     KDIVAGRVRIFETVPSMLGAMLDTPECEQLAGSELRYLISNAEALPPGMCDRFRQKLR
                     PIRLLNAYGPTECSDDVTHFEITTVEGFDNVPVGSPIANTQLYVLDQGYELSPQGVIG
                     ELYIGGTGVARGYLNRPALTAEKFVPDPFATVPGTRMYRTGDVVRWREDGNLEFLGRA
                     DQQVKLRGYRIELGEVEAVMSQHVGVQQSVIVVRDEALIGYVVGTAEVTELREYLAKQ
                     LPSYMVPRLIKLVSLPLTSNGKVDRKALPAPEQWEETEQAGPRTPVEELLCGIWAEVL
                     GREQVGREENFFELGGHSLLATKVVSRVRAVFGVEVALRVLFERPTVAAMAAEVELAQ
                     AGGTAMPALERVERDGPLPLSFAQQRLWFIEQLEPGNTAYNVPLAVSLSGELDYAALG
                     AALSEIVRRHEVLRTRFVNEDGEAVQVIEAARAVLVPVIDVAELGETAGEVAAKRIAQ
                     VGAKRAFELSEGELLRPVLVRLGAREHVLVVVLHHIVSDGWSVGVLISEFNLLYEAYR
                     QGETSPLAELPLQYGDYAVWQRNWLQGEVLERELQYWRRQLNGLERLELPTDKSRSEM
                     TGRSEVVRFEIEPELLDRLRQLSRREGVTLFMLLAAGLKLVLSRYAEQSDVAIGTAIA
                     NRNRVEIEPLVGFFVNQLVLRTRVDRDLSVRDLLQRVRETVLGAYAHQDLPFERLVED
                     IAPSRDVFRSPLFQVMLVMQNFVNVEMQLEGLTTANLNIGNGDAKYDLMLTLDERAEE
                     IVAELEFSTDLFTRATIERFGEHYLHVLDACTHNVEQKLGSIKLTSEAETARLIAKVN
                     AAARPFPIECVHESFRRQAAKTSNAIAVEDDQISWTYAELDRYSEYVAHNLVKARVSP
                     GSLVAICLERSVEMVAAILGVLKAGAAYVPLDSNYPAERLEYMLRDAGVAATITDDRL
                     NVSGPKLLVSDLLLETSGQQLPHVFPDTLAYVIYTSGSTGAPKGVAITHAGLSNHMSW
                     FDEAFALGAQDRVLQKTVVSFDASVWEFYAPLLTGGTLVMASPGAHADPATLIENIRR
                     KRVTIFQVVPTMLRLLVNHAGWNECTSLRFVF"
     gene            complement(5336..6271)
                     /locus_tag="VFH96_09460"
     CDS             complement(5336..6271)
                     /locus_tag="VFH96_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HET6974239.1"
                     /translation="MFKQIKFLRSNNPPGTSFMRYTAARAGAAPAKIRDWWSLDRIDP
                     VFACAVVKSEDFCFSKHYGVAWEATLFATIAALRRKNEIKGVSTITQQLARNLFLTPE
                     RKISRKLREIVYAIAMDRSLGKQRVLELYLNTVEWAEGVWGCAAATQHYLGKNPDQLS
                     PFEGIVLSSLLPAPRRALTGANLERACAVQNRVAWQLYLSGVLEKPEMIECLSRVTRL
                     RELLAQGRSLTGAFLAERTPFAPPMDSVLVPLTRQKAERLLPEDIVAQEYGIERERFE
                     LIELRRSLGTEAWRELVMSGKFFRLAATDAEPNLT"
     gene            complement(6287..7138)
                     /locus_tag="VFH96_09465"
     CDS             complement(6287..7138)
                     /locus_tag="VFH96_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6974240.1"
                     /translation="MENNDTKAVSVIITWCNRPEIETFLAHNGPEFNSVDAEVIVMNC
                     SGDSEALAAAIKRSGYQGVRQIDIAMPKFNKCVAINLGVHLSHGQTIFMNDTDMLLDS
                     GFLKQAVSLVSASNYVTVERVVEKEGTEATPSYLMIMSQTTEFTCRDGRIIRFENERI
                     YLDDNSRVGYGQVVVRKEDFLAAGGMNSQLQGWGYEDQDLHLRLNAVHGLTVVRVGRA
                     THLTHGDNKRDLVGETKSESHRRNLAIGYDNYARGNFMGTYAADVAQWSERSSERVAV
                     TEEVLTY"
     gene            complement(7165..>7465)
                     /locus_tag="VFH96_09470"
     CDS             complement(7165..>7465)
                     /locus_tag="VFH96_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974241.1"
                     /translation="TREAQLGIDYLLPLREIQEYQQRVDARMMPVPVIPPQDVSPWIE
                     TLRRLTEDRADYERVSRGSRAAALAHVETQTVDALENYLHSLQGSHVEKVSTASW"
BASE COUNT         1598 a         2406 c         1923 g         1538 t
ORIGIN      
        1 gaacacgaaa cgaagcgagg tgcattcgtt ccaaccggcg tgatttacga gcagccgcag
       61 catcgtcggc actacctgga aaatcgtcac ccgcttacgg cgaatgttct cgatcagcgt
      121 cgcgggatct gcatgcgctc ccggactggc catcaccaga gttccgccgg tgagcagtgg
      181 cgcataaaac tcccacactg aagcatcgaa actcaccacc gtcttctgga gcacgcgatc
      241 ctgcgcgccg agcgcaaatg cttcgtcgaa ccacgacatg tgattgctca accctgcgtg
      301 agtgatcgcc actcccttgg gggcgccagt cgaacccgac gtgtaaatga cgtaagcaag
      361 tgtgtctggg aagacatgag gtagctgctg accggacgtt tctaacaata aatcactgac
      421 caacagcttc ggccctgaaa cgttcagccg gtcgtctgtg atggtcgccg ccacgcccgc
      481 gtctcggagc atgtactcga gccgttcggc gggatagttg gaatcgagtg gaacgtacgc
      541 cgctcccgct ttgagcacgc cgaggatcgc cgcaaccatc tcgacgctgc gctcgagaca
      601 aatcgcgacc agcgaacccg ggctgacacg cgccttcacc aaattgtgag caacgtactc
      661 ggaataacga tcgagctcag catacgtcca tgagatctga tcgtcttcga ctgcaatcgc
      721 gttcgacgtc ttcgcagcct ggcgccggaa cgactcgtgc acacattcaa tcggaaacgg
      781 acgtgctgcg gcattcactt ttgcaatgag tcgcgcagtc tcagcctctg acgttagctt
      841 gattgagccg agcttctgtt ctacgttgtg ggtgcatgcg tccagcacat gcaagtaatg
      901 ctcaccgaat ctttcaatcg tggcgcgcgt aaacaggtcc gtgctgaatt ccagctccgc
      961 aacgatctct tcggcacgct cgtcgagagt caacatcagg tcgtacttcg catcgccatt
     1021 accgatgttg agattcgcag tcgtcaatcc ttccaactgc atttccacgt tcacgaagtt
     1081 ctgcatcacg agcatcacct ggaagagtgg cgaccggaac acgtcgcggc tcggggcaat
     1141 gtcttcgacc aatcgctcaa acggcagatc ctgatgcgcg taagcaccga gcacagtctc
     1201 acgcactcgc tgcaatagat cgcgaacgct cagatcgcgg tctacccgcg ttcgcagcac
     1261 caactggtta acgaagaagc cgaccagcgg ctcgatctca acgcgattgc gattggcgat
     1321 cgccgttccg atggcgacgt cgctctgttc tgcatagcgg ctcaacacca atttcagccc
     1381 ggccgccagc agcataaaca gcgtcacgcc ttcacgccgg ctcaactgtc gcagtcggtc
     1441 caacaattcc ggttcaatct cgaagcgaac tacttcgcta cgtccggtca tttccgaacg
     1501 tgatttatca gtgggcaact cgagacgttc aagtccattc agttgccggc gccagtactg
     1561 caactctctt tccagcactt cgccctgcaa ccagttgcgc tgccacactg cgtagtcgcc
     1621 atactgcaac ggcaactcag ccaacggcga tgtttctcct tgtcgatatg cttcgtagag
     1681 caggttgaac tcgctgatca gcacgcccac tgaccaaccg tcgctgacga tgtggtgcag
     1741 caccaccacc aacacatgct cccgcgcgcc caaccgcacc agcaccgggc gcagcaactc
     1801 gccttcgctc aactcgaaag cgcgcttagc tcccacctgc gcaatacgct tcgccgctac
     1861 ttctccagcc gtctctccca attccgctac atcaatcacc ggcacgagca ccgcacgcgc
     1921 ggcttcgatc acctgcacgg cctcgccatc ttcgttcacg aaacgcgtac gcaacacttc
     1981 atgtcgtcgc acgatctccg acagcgccgc tcccagtgct gcgtagtcca actctccact
     2041 caaactcact gccagcggca cgttgtaagc tgtgttgccc ggctccaact gctctatgaa
     2101 ccacaaacgc tgctgcgcaa acgacaacgg caacggcccg tcacgctcaa ctcgctccag
     2161 cgccggcatt gccgtgccac ccgcctgcgc cagctccact tcagccgcca ttgccgcgac
     2221 tgtcggccgc tcgaacaaca ctctgagcgc gacttctact ccgaacacag ctcgcacccg
     2281 cgacactact ttcgtcgcca gcaacgagtg tccacccaac tcaaagaagt tctcttctcg
     2341 cccgacctgc tcgcgtccca atacctccgc ccagatgcca cacagcagtt cttccaccgg
     2401 cgtgcgtggt cctgcctgct ccgtctcttc ccactgctcc ggcgctggca acgctttccg
     2461 gtctaccttc ccattgctcg tcaacggcag actcaccagc tttatcaaac gcggcaccat
     2521 gtacgacggc aactgcttcg caagatactc gcgcaactct gttacttccg ccgtccccac
     2581 cacataccca atcagcgcct cgtctcgcac cacaatcacg gactgctgca cgcccacatg
     2641 ctgcgacatc actgcttcca cttcacccag ctcgatgcga tagccacgca gcttcacctg
     2701 ctgatctgct cgtccgagaa attccaggtt cccatcttct cgccaccgca ccacatcacc
     2761 cgtgcgatac atccgcgtgc ccggcacagt cgcaaacgga tcgggcacaa acttctcagc
     2821 cgtcagtgcg ggacggttca ggtaaccacg cgccacacca gtaccgccga tgtacaactc
     2881 gccaatcacc ccttgagggc tcaactcata accctggtcc aacacgtaca actgcgtgtt
     2941 cgcgatcgga gagccgaccg gtacgttgtc gaacccctcg acagtggtta tctcaaaatg
     3001 cgtaacgtcg tcggaacact cagtcggacc atacgcgttc agcaaacgaa tcgggcggag
     3061 cttctggcga aagcgatcac acatacccgg cggcagcgct tcggcattcg aaatcagata
     3121 ccgcagctcc gaccccgcaa gctgctcgca ttccggcgtg tcgagcattg ctcccagcat
     3181 cgatggaacc gtctcgaaaa tcctgacccg gccggccaca atgtccttta ttaataggac
     3241 cggatcattt gactctgctt ccgagacgat acggatacac gcgccggtga gaagcggcgt
     3301 cagcatctgc cagattgaaa tatcgaacga gatcggcgcg ttgaaagcga cgacgtcagt
     3361 tgatccaagc tccagcgcct taaccttcgc cagcagatga ttcaacatgc cgccacgttg
     3421 gaccatcacg cccttaggcc gtcccgtcga tccggaagtg tagataatgt aggcaagcct
     3481 gttccgaggc accggcgttt cgacttcgaa accgtcatta ccagcgctga tttcgtccaa
     3541 acaaacaacc tgcacggatg cagcatcgtc agcgaagcca cgaaccgtca acaccagctt
     3601 tacgctcgca tcgctaagaa tctcctgcac acgttcgcgt ggataggcgc ggctaatcgg
     3661 caggtaactc acacctgctt tcaggcaggc gaggagcgaa acaaccactt ccgggctgcg
     3721 ctccaacatg acggcgacgg cgtcctcggt ttccagtgat gcttcgcgaa tgatgttggc
     3781 gatcctgttg gaccatgcgt ccagttcgct gtacgtgatc gtctggtcgc caaaggtcac
     3841 cgccgccgcg tggggagcgt ttccaactga cccacggaaa agttgcacga agtctttgtc
     3901 aggcagtggt tgttccggcc cggtcccgag cgcgaacaaa ctatcgactt ccgcctggtc
     3961 cagtagcact ctcgagatcg gctgtcccgg attcgatgca ccggctcgca gcaggttttc
     4021 atagttggtg agaaaccgcg tcaacggctc gccatcaaac aaatccttcg cgtagaccag
     4081 ccggatctga atgctcccct ggctctcgac gacaagaaac tccagcggga aaactgcctt
     4141 gtctttgagt acgccatcct ccggctcgaa acgcaccccg cccgacatcc gcgacgcggg
     4201 cagctcggcg ccgaggaaag tccacgccac ctccagcaaa ggctcgatag ccactccatg
     4261 cctttcggca acacggctga atggcacatc accgcgcgcg taaacttcga acgcgcgccg
     4321 cgaaactcgc gatagcagct cggcaaacga cgacttaccg ggcacgtcaa ccggcatcac
     4381 gaccgtgttc acaaacagcc cgatcaactt ctgcaattga ggtttgtgcc gggcagccag
     4441 tggtagcgcg atagtgatgt tgctctggct tgtgtagcga ctgagcaaac atgcgagacc
     4501 agtcaacaaa acgttggcaa tcgtggttcc cgatttgcgc gccgtttgtg agagcgattt
     4561 gacgaattgc ggatcgagcg tgagcgtatg cgcgcccgcc gcgagcgtgt tctgatcgga
     4621 tcgtttcctg aatggcagca gtggcgggcg cgcatcctct aacgcactgc gccaatactc
     4681 agcttcacgg tccagacgtt cgtcagtaaa cgagcggcgc tcccaggctg caaacgctga
     4741 atactgcgcc gcgaccggcg gcagcgccgg ttctttaccg tccctcagag cctcgtagat
     4801 gaggcacaac tcgtcgatga agatcatcgc ggaggtgcgg tcgcaagcga tgtggtggac
     4861 tccaatcgcc aaaaagctct ggtcaggcga acactggaac agaacagcgc gcaccggagg
     4921 ctctcgatcg agacgaaacg gattctctct cacgcgctcg agttctcgac ggagtatttc
     4981 cagcgccact gatgcatcaa acgcggagag gtcgacgaca ttaagggcaa gaattgaggc
     5041 cggttgcgcg gtctggcacg gcccctcagc acgcagcgaa taagtggtgc gaagaatctc
     5101 atgacgctcc gccagttgga ccaacgcttc agcaaacacc gccggacgca atggtcccac
     5161 tacgcgatga actatcacct cgttgtagcg atagctctcg ggctcaattg cttgtgccag
     5221 ccataaccgc tcctgcgcaa aagacagcgg atggtctacg tttcggtcgt tcatcatttt
     5281 tggaattttt ggcgcgtatc cttcggcttc gcgccggctg tgagcagcca gctttttacg
     5341 tgaggttggg ttcggcatcg gtggcagcga ggcggaaaaa ctttcctgac atgaccagct
     5401 cgcgccatgc ttccgttccg agactgcggc gcaactcgat cagctcaaat cgttcgcgct
     5461 cgataccata ctcctgggca acgatgtctt caggtaacaa acgttccgct ttctgccgtg
     5521 tcagcggcac cagcacgctg tccattggtg gagcgaatgg cgttcgctcc gctaaaaagg
     5581 cgccggtcaa cgatcgtccc tgcgccaata actccctcaa ccgcgtgacg cgcgacaggc
     5641 attcgatcat ttccggtttc tcgagcacgc cggaaagata aagttgccag gccacgcgat
     5701 tctgaactgc gcatgctcgt tcgaggttag cgccggtgag cgcccggcgc ggcgccggca
     5761 ataacgacga caaaacgatc ccctcgaacg gactcaactg gtccggattt tttccgagat
     5821 agtgttgcgt cgccgcggca caaccccaaa caccctcggc ccactcgacg gtattcagat
     5881 acaactccaa aacgcgttgc ttgccgagcg aacgatccat cgcgatcgca tagactatct
     5941 cgcgcagctt gcgggagatc ttgcgctccg gcgtgaggaa aagattgcgc gccaactgct
     6001 gcgtgatcgt gctcacgcct tttatttcat tttttcttcg caacgcggcg attgttgcaa
     6061 acagcgtcgc ttcccacgcg acgccgtaat gtttgctgaa gcaaaagtct tccgacttca
     6121 ctaccgcgca agcgaaaacg ggatcgattc gatcgagcga ccaccagtca cgaatttttg
     6181 ccggcgcggc gccagcgcgc gcagccgtgt agcgcatgaa gctggtgccg gggggattgt
     6241 tgctgcgcag gaatttgatc tgcttgaaca tcgcaaaaaa ctttcgtcag taggtcaaca
     6301 cttcttccgt cactgccaca cgctcgctgc tgcgctcgga ccattgcgcc acgtcggcag
     6361 cataagttcc catgaagttt ccgcgcgcat agttgtcgta accgatggcg agattgcgcc
     6421 ggtggctctc ggattttgtc tctcctacga ggtcgcgctt attgtcgccg tgcgtcaggt
     6481 gagtcgcgcg accaacgcgc acaactgtca gtccgtgaac ggcgttcaga cgcaggtgca
     6541 gatcctggtc ctcgtagccc cagccttgca gctgggagtt catcccgccg gcagccagaa
     6601 aatcttcctt gcgcacgacg acctggccgt atccgacgcg gctgttgtcg tcaagataga
     6661 tacgctcgtt ttcaaagcga atgatccggc cgtctcgaca ggtaaactcc gtcgtctgac
     6721 tcatgatcat cagatacgac ggcgtggcct cggtcccttc tttctccacc actcgctcga
     6781 cagtcacgta gttgctcgcg gacaccagcg acaccgcctg tttcagaaac cccgaatcga
     6841 gcagcatgtc cgtgtcgttc atgaatatag tctggccgtg agacaagtgg acgccgagat
     6901 tgatcgccac gcacttgttg aacttcggca tcgcgatatc gatctggcgc acgccctggt
     6961 atccggaacg tttgatcgcc gcagccaaag cctcgctgtc accggagcag ttcatcacga
     7021 tgacttccgc atcgacggag ttaaactccg gaccattgtg cgcgaggaat gtttcgatct
     7081 cgggtcgatt gcaccaggtt atgattaccg aaacggcttt cgtatcgttg ttttccatca
     7141 aattctcctc agttgaaatg acttctacca cgaggcggta ctgaccttct ccacgtgact
     7201 accctgcaga ctgtgtaaat aattttcgag cgcgtcgact gtctgcgtct cgacatgggc
     7261 cagcgccgcc gctcttgatc cgcgcgacac acgctcgtag tcagcacgat cttccgtgag
     7321 acgccgcaat gtctcgatcc acggtgaaac gtcctgcggc ggtattacgg gaacgggcat
     7381 catgcgcgca tctacgcgct gctggtattc ctgaatctcg cgcagcggca atagatagtc
     7441 gattccgagt tgcgcctcgc gcgtg
//
LOCUS       DASSFQ010000264         8811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1904358, whole genome shotgun sequence.
ACCESSION   DASSFQ010000264 DASSFQ010000000
VERSION     DASSFQ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904358"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFH96_09475"
     CDS             <1..655
                     /locus_tag="VFH96_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HET6974242.1"
                     /translation="DRVTQPVTVRTYYDRTSDVAQLNIYDTNREDTGGVGDVATGTFT
                     VPNGTELVAVLNNDLSTQNVREGERFTMTVRSPGQYDGATVEGSVVSINRGGRITGRS
                     EMTLDFDTIRMRDGRSYRFAGILESVRTPNGDVVRVDNEGAVRDSDQTNKTIQRTAIG
                     TAVGAIIGAIAGGGKGAAIGAVIGAGAGAGSVYVQGPNDLDLTAGTELTVRATGPRG"
     gene            complement(701..1282)
                     /locus_tag="VFH96_09480"
     CDS             complement(701..1282)
                     /locus_tag="VFH96_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974243.1"
                     /translation="MTRPALFLLLLAALSSASCTAASLYKVKPVTELPPMPANTRSAD
                     AGGVTIRVAPLLTDEESQELFEANLPLSGVLALRVELAFQSGVPVEIKRARIRLRDSE
                     NKEWKLLSAKSAVSRIMKANGVYAYNPNSRKQFENEFGAYAVDLKTPLSSGDSRRHGF
                     LFFQTPDKRPVRSGQQLTLTVERLPQPASITIN"
     gene            1320..1838
                     /locus_tag="VFH96_09485"
     CDS             1320..1838
                     /locus_tag="VFH96_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET6974244.1"
                     /translation="MIRPFRGVHPQIHPTAYVDPSAQVIGDVHLGEKASIWCNCTVRG
                     DIHYIRIGDRSNVQDNSVIHVRNGTHPTILENEVTVGHSVTLHGCYVERGCLIGIGAI
                     LLDDVRIGELSLVAAGSLVSPGTIIPPRSLVMGFPAKVKRPLTDEEVAGLEVFWKNYV
                     EYTEEYRKEMML"
     gene            1866..2522
                     /locus_tag="VFH96_09490"
     CDS             1866..2522
                     /locus_tag="VFH96_09490"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HET6974245.1"
                     /translation="MTRFSKFIFTLAVLICASVPVFAQTSDNPFLLKRGDNEWGFWAG
                     FSPKATTIFEGLHEDEAADRKFFIAALRYGRTLAANNSLALQYTLDVVPVAVATGVIV
                     SRTTSPTGVDTFRRETAYGAGFTPLGLQLDFANGRKVHPFVHVNGGLLYFNKDVPIED
                     SGRFAFVGEAGGGVRIFTSERRAVNIGVRFHHISNGDTSGSNRGLNQFVIYAGFSIFK
                     "
     gene            2522..3919
                     /gene="hisS"
                     /locus_tag="VFH96_09495"
     CDS             2522..3919
                     /gene="hisS"
                     /locus_tag="VFH96_09495"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET6974246.1"
                     /translation="MPSAKTQPARGMRDFLPADVRKREYVIDLVKEVYESYGFEPLET
                     PAVENIETLMGKYGEEGNQLIFKILKRGVHESTGEADLALRYDLTVPLARVVAEYRDK
                     LPKFFKRYQIQPVWRADRPARGRFREFYQCDVDVLGSRSTIVEAEIISAASEVLSRLG
                     FKDFCVRLNHRKVLTGILGIAGVSLDKHESALIALDKLDKIGSEGVKKEFEARGVAET
                     AGDRLLSFFSALSELEHAAEIVTEDNAQQAFNKAVLGRIVEFVKDNELGAQGVDDLQS
                     ILDYADVMGIGHRVKIDPTLARGLSYYTGAIMEINVKDLAGSLGGGGRYDNLVGMFLG
                     QDIPACGFSLGLERILVVMNERNMFPPDLGSSPADVMVAIWNKDSVAESIKLARELRA
                     GGLSVDLYPEADKLGKQFKYASSIEVPFVAVIGEEEQAKGLVAIKNMASGEQRAVERD
                     NVVKEIVAQRSKVKS"
     gene            3951..4553
                     /locus_tag="VFH96_09500"
     CDS             3951..4553
                     /locus_tag="VFH96_09500"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HET6974247.1"
                     /translation="MSSEDIVQNYHKDAVSAMRAYKKLAEKAIAQLKDEEFFVTLDEE
                     ANSVAVVMKHMAGNMISRWTDFLTSDGEKPDRNRDMEFVIDATTSKDDVLAYWERGWK
                     CVLDAIEPLTPEDFEKTVLIRGEKHTIVQAINRQLMHYAYHIGQIVFLAKHFRSAEWT
                     SLSIPRNRSADFNAYLIAQPQAVADREKQFDAAAGFAEKK"
     gene            4550..4918
                     /locus_tag="VFH96_09505"
     CDS             4550..4918
                     /locus_tag="VFH96_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6974248.1"
                     /translation="MNAQIHHVNVTVPKSLEDAAKHFYGVVMGLEEVPKPEGSRGRGG
                     AWYELGPLQLHLSIEGPLHPDCISKRHVCYTVSDLAQAEERFRNAGVEIIPDDLPTPG
                     WSRFYVRDPGGNRLEIAQAT"
     gene            4929..6737
                     /gene="aspS"
                     /locus_tag="VFH96_09510"
     CDS             4929..6737
                     /gene="aspS"
                     /locus_tag="VFH96_09510"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET6974249.1"
                     /translation="MLDNLGNLERTHSCGELRAQDIGKQVVLMGWVAKRRDFGELTFV
                     DLRDRDGITQVVFNAEDAPEAHTKAKELRGEYVIAVGGEVLARAENTRNPKLPTGEVE
                     VHARELLILNDARTPPFQLDAAPKSLASEDLRLKYRYLDLRRPQLQANLVLRHRVTNE
                     IRRYMSAQGFTEIETPIMVKSTPEGARDYIVPSRIFPGKFFALPQSPQLFKQLCMIGG
                     LDKYFQIAHCFRDEDLRADRQPEFTQLDVEMSFATLDQIYNLIEGLFASVFKLINVEL
                     PGPFPRFTFAEVMRRFGSDKPDLRIQGVELQDLATSVEGTDFPPFASALSSGGQVKGI
                     VVPGGANLSRKVLDELQEFAKRYGAGALAWIKLGDETTSSLLKALGADVVARMADVAG
                     AKKGDAVLIVSGKPKIVAASLGALRNEIAKRENLIPANVYAPLWVTDFPMFEFHEEDG
                     RYYSMHHPFTSPVDEDLELLQRAVEGGEANLLGQIRTKAYDPVINGVEMASGSIRIHR
                     RDVQRLVFKALGMSTEEARERFGFFLDALEYGTPPHGGIAPGIDRLMMVLAGTDNIRD
                     VIAFPKTASAADLMIDAPGEVDPKLLEELHLRVVED"
     gene            6740..7159
                     /locus_tag="VFH96_09515"
     CDS             6740..7159
                     /locus_tag="VFH96_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET6974250.1"
                     /translation="MPLLQLRREHVDAMIAHARETAPEECCGLIGGISETRAVTLYRL
                     RNVTPHPQSAYEAAPEDLFAAQRQMRERGEDLLAIYHSHPRASDPNPSETDVRLAYYP
                     TAKYLIIGLAGIEAVIKAFSISEREHRWQQVEYEIAD"
     gene            7296..7994
                     /locus_tag="VFH96_09520"
     CDS             7296..7994
                     /locus_tag="VFH96_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974251.1"
                     /translation="METKKTKYDTNPLDPDVERKAEDVWGDLGGITQQVGGTTQQVSN
                     EPPRPQHVEDARKNVYSEAPTRRYDNPPLEAPYPSIFVPPTYAPPPAQYQSRQNIYQP
                     PVSAASSSRPVLGIGIPEKWATMLAYTPGYIGLVVGLLELFLVPRSEGKVRFHASQGV
                     ALHIAFLIVQTIFSVISMLTGSTIGGTMFKLAAFIFLIISMIRVWKGEPHQITPLAEP
                     AQWFNQHIEPRNRG"
     gene            8188..>8811
                     /locus_tag="VFH96_09525"
     CDS             8188..>8811
                     /locus_tag="VFH96_09525"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET6974252.1"
                     /translation="MFKRQTLIAWLTLVAISVMPFTALAQATQIQYHSNKYSIQDDIK
                     LGRQAAQEAESQFPLLRDQEVQSYVESVGRRLVTAIPSQFQHPEFQYYFKVVNARDIN
                     AFALPGGPMYVNRGMIEAARTEGEMAGVMAHEISHVALRHGTAQASKGQKYSILGGIA
                     GIAGTILGGPGVGQLAQAPFAVYLLKFSREYETEADILGAQIMAQAGY"
BASE COUNT         2001 a         2287 c         2566 g         1957 t
ORIGIN      
        1 cgatcgcgta acgcaaccag tgacagtcag aacttactac gaccggactt ctgacgtcgc
       61 gcagttgaac atttacgaca ccaatcgcga agacacgggc ggcgttggtg acgtggcgac
      121 gggcacattc acagttccga atggaacgga gctcgtcgca gtgctgaaca acgatctgtc
      181 gacgcagaac gttcgcgagg gcgaacgctt cacgatgacg gttcgatcgc cgggccagta
      241 tgacggtgcg acggttgagg gttcagtggt gagcatcaac cgcggcggcc gcattaccgg
      301 acgctctgag atgacgttgg acttcgacac gattcgtatg cgagacggcc ggagctatcg
      361 ctttgccggc attctcgaat cggttcggac gccgaatggc gacgtggtcc gtgtcgataa
      421 cgaaggcgcg gttcgcgaca gcgaccagac caacaagaca attcaacgca ccgccatcgg
      481 tacagcggtc ggcgccatta ttggagccat tgcgggcggt ggtaaaggtg cggcgatcgg
      541 tgcggtgatt ggcgccggcg ctggtgcagg atcggtatat gttcaaggtc caaatgatct
      601 tgatctgact gccggaactg agctgaccgt gagggccact ggtccgcggg gttagtcaca
      661 aatgcgagta taggtcctat atgtcctata ggacctatac ttagttgatg gtgatcgatg
      721 ctggttgggg caggcgctct acagttaacg ttaactgctg gccggagcgc accggacgtt
      781 tgtccggagt ttgaaaaaat agaaaaccgt ggcgacgcga atctcctgac gacaacggcg
      841 tcttcaaatc gacagcataa gcaccaaact cattctcaaa ctgcttgcgc gaattcggat
      901 tgtaggcgta aaccccgttc gccttcatga ttctcgaaac cgcactcttc gctgacagta
      961 gtttccactc tttgttttca ctgtcacgca gccgaatgcg tgcccgcttt atctccaccg
     1021 gcacaccact ctgaaacgcg agctcgacgc gcaacgccaa cacaccgctc aacggcaggt
     1081 tcgcttcaaa gagttcctgg ctctcttcgt ctgtgagcaa cggcgcgaca cgaatcgtca
     1141 cgccacctgc gtctgcgctg cgcgtgtttg caggcatcgg cggcagctca gtcaccggct
     1201 tcaccttata gagcgaggcg gcggtacacg acgctgagct gagggcagcg agcaggagca
     1261 ggaaaagtgc gggtcgggtc atggcctcaa tttacactat gttactatct gccgaaacca
     1321 tgattagacc gttccgtggg gttcacccgc agattcaccc aaccgcgtat gtcgacccca
     1381 gcgcccaagt gataggcgac gtacacctcg gagaaaaggc ttcaatctgg tgcaactgca
     1441 ccgtccgcgg cgatattcat tacattcgca tcggcgaccg ctccaacgtg caggacaatt
     1501 ccgtgattca tgtgcggaac ggcacacatc ccacgatact cgaaaacgaa gtgaccgtgg
     1561 gccatagtgt cactctgcac ggatgctacg tcgagcgcgg atgtttgatc ggaatcggcg
     1621 cgatcctgct ggacgacgtg cgcatcggcg agttgtcgtt ggttgcggcc gggtcgctcg
     1681 tgagtccagg cacgatcatc ccaccgagat ctctggtgat gggatttccg gcgaaggtga
     1741 agcgcccgtt gacggatgaa gaagtggctg gcctcgaggt gttttggaag aattatgtgg
     1801 agtacaccga ggagtacagg aaagagatga tgttgtaaga aaaacttttg tctgaaggac
     1861 tttaaatgac tcgtttctca aaattcattt tcacgttagc tgtcttgatt tgcgcgagtg
     1921 tgcctgtctt tgcacaaacc agcgacaacc cgtttctcct caaacgaggc gacaacgagt
     1981 gggggttctg ggccggcttt tcaccgaaag caacaacgat ctttgaaggt cttcatgagg
     2041 acgaagccgc agatcgcaaa ttcttcatcg ccgcattgcg gtacggccgc acgctggctg
     2101 ccaataactc gctcgcgttg caatacacgc tcgacgttgt tccagtcgca gtagccaccg
     2161 gcgtgatcgt ctcccgcacc acgagcccga ctggagtcga cacttttcgg cgcgagacag
     2221 cttacggcgc cggattcacg cctctgggtt tacaactaga tttcgcaaac ggcaggaaag
     2281 ttcatccgtt tgttcacgtc aacggcggct tactttattt caataaagac gtgccgattg
     2341 aggactcggg acgttttgct tttgttggtg aagcgggcgg cggcgtgcgc atctttactt
     2401 ccgagcgtcg tgcggtaaat atcggtgtca ggtttcacca catctcgaat ggagatacgt
     2461 cgggttcgaa tcgcggcttg aatcagtttg tgatttacgc cggtttctcg attttcaagt
     2521 aatgccttct gcgaaaacgc aaccagcgcg cggcatgcgc gacttcctgc cggcagacgt
     2581 tcgcaaacgc gagtacgtca tcgatctggt caaggaagtt tatgagagtt acggtttcga
     2641 gccgttggaa acgccggccg ttgaaaacat cgagacgttg atgggcaagt atggcgaaga
     2701 agggaaccag ctcatcttca agatactcaa gcgaggcgtg catgagtcaa cgggcgaagc
     2761 tgatctcgcg ctgcgttacg atctgactgt tccactcgcg cgcgtggtgg cggagtatcg
     2821 cgacaagctg ccgaagtttt tcaagcgtta tcaaatccaa ccagtgtggc gggcagaccg
     2881 gcctgcgcgc gggcgttttc gagagtttta ccagtgtgac gtcgacgtgc tcggctcgcg
     2941 ttcgacgatt gtcgaggcgg agattattag tgcggcgagt gaggttttat cgcggcttgg
     3001 atttaaagat ttctgcgtgc gactcaatca tcggaaagtc ttaactggaa ttctcggcat
     3061 cgccggtgtt tcgctggaca aacacgaatc cgcgctcatc gcgttggata agttagacaa
     3121 gatcggtagc gagggagtca agaaagagtt tgaggcacgc ggtgttgcag agactgccgg
     3181 tgaccgattg ctgtcgttct tctcggcttt atctgagctc gaacatgctg ctgagattgt
     3241 gactgaagac aatgcgcaac aggcgttcaa taaagccgtg ctgggccgta ttgttgagtt
     3301 tgtgaaagac aatgagctcg gcgcgcaggg cgtcgatgat ttgcagtcga tactcgatta
     3361 cgctgacgtg atggggattg gtcatcgggt gaagatcgat ccgactcttg cgcgtggact
     3421 ttcttactac accggcgcga tcatggagat caatgtgaag gatctggcgg ggagtcttgg
     3481 cggcggtggg cgctatgaca atctcgtcgg gatgtttctc gggcaggaca ttcccgcgtg
     3541 tgggttctcg ctcgggctcg aacgcatact cgtcgtgatg aatgagagaa atatgtttcc
     3601 gccagacctg ggttcgtcgc cggctgatgt gatggtggcg atctggaata aagatagtgt
     3661 cgcggagtca attaagttgg cgagggaatt gagagccgga ggtttgtcgg ttgatttgta
     3721 ccctgaggct gataagctcg gcaagcagtt caaatatgcc tcgtcgattg aggtgccgtt
     3781 cgttgctgtg attggtgaag aagagcaggc gaaggggttg gtggcgatca aaaacatggc
     3841 aagcggtgag caacgcgcgg ttgagcgaga taacgtcgtg aaagagatcg tggcgcagcg
     3901 gagtaaagtt aaaagttaga caggattaca ggattggcag gattaacaag atgtcgagtg
     3961 aagatatagt tcagaactat cacaaggatg ccgtgtcggc gatgcgggcg tacaagaagc
     4021 tcgcggagaa ggcgattgcg cagttgaaag acgaagagtt ttttgtaact ctcgacgagg
     4081 aagcaaacag cgtcgccgtg gtcatgaagc acatggccgg gaacatgatc tcgcgttgga
     4141 ctgactttct cacttccgac ggcgagaagc ccgatcgcaa tcgcgatatg gaatttgtca
     4201 ttgatgcgac gaccagcaag gacgacgtgc tcgcgtactg ggagcgcggc tggaaatgcg
     4261 tcctcgatgc aatcgagccg ctgacgccgg aagacttcga aaagacggtg ttgattcgcg
     4321 gtgaaaagca cacgatcgtg caggcgatca atcgccaact gatgcactac gcgtatcaca
     4381 tcggccagat cgtttttctc gcgaagcact ttcgttcagc ggagtggaca tcgttgagca
     4441 ttccgcgaaa tcgctcggcg gactttaatg cgtatctaat cgcgcagccg caggccgttg
     4501 ccgatcgaga gaaacagttc gacgcagccg ctggatttgc cgaaaagaaa tgaacgcgca
     4561 gatacatcac gtgaacgtca ctgttccgaa gtcgctcgaa gatgcggcga agcatttcta
     4621 tggcgtcgtg atgggtctcg aagaagtgcc gaagccggaa ggctcgcgcg ggcgtggcgg
     4681 cgcgtggtac gaactcggcc cgctgcagct tcacttatct attgaaggac cgttgcatcc
     4741 ggactgcatc agcaagcgac acgtttgtta caccgtcagc gaccttgctc aagctgagga
     4801 acggtttcgg aatgcaggcg tcgagatcat acctgacgat ttgcccacgc ccggctggtc
     4861 ccgcttttat gttcgcgatc ccggtgggaa tcgtttagag atcgctcagg ctacttaacc
     4921 acagatttat gctcgataac ttaggaaatt tagaacgcac tcactcttgt ggagaactgc
     4981 gcgctcagga catcggcaag caagtcgtgc tgatgggttg ggttgcaaaa cgtcgtgatt
     5041 ttggcgagct cactttcgtc gatcttcgcg atcgcgacgg tatcactcaa gtcgttttca
     5101 atgccgaaga cgcgccggaa gcgcacacga aagccaaaga gttacgtggc gagtatgtga
     5161 ttgcagtcgg aggcgaagtt ctggcgcgtg ctgagaatac acgcaatcca aagcttccga
     5221 cgggagaggt cgaggtccac gcgcgcgagt tgttaatact gaacgacgca cgcacgccgc
     5281 cgtttcaact tgatgccgct cccaaatcgc tcgcgagtga agacttgcgg ttgaagtatc
     5341 gctacctcga tctgcgccgg ccacagttgc aggcaaacct ggtgcttcgc catcgtgtga
     5401 cgaacgagat ccggcgctac atgtcggcgc agggcttcac cgagatcgaa acgccgatca
     5461 tggtcaagtc aacgcccgaa ggcgcgcgcg attacatcgt gccgtcgcgt attttcccgg
     5521 gcaagttctt tgcactaccg cagtcaccgc aactcttcaa acagttgtgc atgatcggcg
     5581 ggctcgataa gtacttccag atcgcgcact gttttcgtga tgaagacttg cgcgccgatc
     5641 gccagcctga gtttacacag ctcgacgtcg agatgagctt tgccacgctc gatcagatct
     5701 acaatttgat tgaaggtctg tttgcctcag tcttcaaact catcaacgtc gaactaccag
     5761 ggccatttcc gcgcttcacg tttgctgaag tgatgcgacg gtttggttca gacaaaccag
     5821 acctgcgcat tcagggagtg gagttgcagg atctcgccac gtctgtggaa ggaactgatt
     5881 ttccgccgtt cgcgtcggcg ttatcgtccg gcgggcaagt gaagggaatc gttgttccgg
     5941 gcggtgcaaa cctttcgcgc aaggtgctcg acgagttgca ggagtttgcg aagcgttacg
     6001 gcgccggagc gctcgcctgg atcaagttag gcgatgaaac tacatcgtcg ttattgaaag
     6061 ctctcggcgc tgacgttgtc gcgcgcatgg cggacgttgc gggtgcgaaa aaaggcgacg
     6121 ccgttctgat cgtttccggt aagccgaaga tcgtcgcggc gagtctgggc gcgttgcgaa
     6181 acgagatcgc gaagcgtgaa aacctgattc ccgcaaacgt ctacgcgccg ctctgggtga
     6241 ctgactttcc gatgttcgag tttcacgaag aagatggtcg ctactattcg atgcaccatc
     6301 cgttcacgtc gcctgtcgat gaagatctcg aattgctgca acgagcggtc gaaggtggtg
     6361 aagcaaatct gctcggccag attcgaacca aggcttacga tccggtgatc aatggcgtcg
     6421 agatggcgag cggctcgatt cgtattcatc gtcgcgacgt gcagcgtctc gtcttcaaag
     6481 cactcgggat gagcactgaa gaagcgcgcg agcgattcgg tttcttcctc gacgcgctcg
     6541 aatacggcac gcctccacac ggcggcatcg cccccggaat cgatcggctg atgatggtcc
     6601 tcgccggcac tgacaacatt cgcgacgtga tcgcatttcc aaagacagcg tcggctgccg
     6661 atctgatgat cgatgcgccc ggtgaagtcg atccaaaact gctggaagag ttgcacctgc
     6721 gagtcgtcga agattgacca tgccgctgct tcaactccgt cgtgaacatg tggacgcgat
     6781 gatcgcccat gctcgcgaaa cggcgccgga ggagtgttgt ggattgattg gcggaatttc
     6841 cgaaacgcgg gccgtcacgc tttatcgcct tcgcaacgtc acgccgcatc cccaatcagc
     6901 ttacgaggca gcgcctgaag atctctttgc cgcacaacgt cagatgcgcg agcgtggcga
     6961 ggatctgctg gcgatttacc attcacatcc gcgcgcgtcc gatccgaacc cgtctgaaac
     7021 cgacgtacgg ctcgcctact atcctacggc caaatacctg atcatcggcc ttgcgggcat
     7081 tgaggccgtc atcaaggctt tctcaatctc cgagcgggag catcgctggc aacaggttga
     7141 gtatgagatc gcagactagt aagattggcc acaaagaggc acaaaaggca caaaagaaag
     7201 ccgaggcaga agatagaatt aggtttctta ccataatcat tatgtgcatt ttgtgccttt
     7261 tcgtggctaa atagttgtgc cttttgtggc tgaagatgga gacgaagaag accaaatacg
     7321 acactaatcc tctcgatccc gatgttgaac ggaaagcaga ggacgtttgg ggcgacttgg
     7381 gcggcatcac tcagcaagtc gggggcacta cgcagcaagt cagcaacgaa ccgccccgac
     7441 ctcagcacgt tgaggacgcg cgaaaaaacg tttactccga ggcgccgact cgtcgttacg
     7501 acaatccacc gctcgaagct ccgtacccgt caatcttcgt tcccccgact tacgcaccac
     7561 ctccggcgca gtatcaatcg cgacagaata tctaccagcc gcctgtcagc gctgcatcat
     7621 cttctcggcc agtactcggc atcggcatcc cggagaagtg ggcgacgatg ctggcttata
     7681 cgcccggata catcggactc gtggtcggac tgctcgagtt gttcctcgta ccgcgtagtg
     7741 aaggaaaagt tcgctttcac gcgtcacagg gcgtggctct tcacatcgct tttctgatcg
     7801 tccaaacgat cttcagtgtg atcagtatgc tgacgggcag cacaatcggc ggaacgatgt
     7861 tcaaattagc agccttcatt ttcctgatta tctcgatgat tcgcgtctgg aagggcgagc
     7921 cgcatcaaat aacgccgctt gccgaaccgg ctcaatggtt taaccagcat atcgagcctc
     7981 gcaaccgggg ctgatcacca tctggcattt tcattgcatt gataactgcc ctgatcggtt
     8041 aatcgcgaaa tatgcgatat ttacgaagtg aaaccgatct ttggcaagca tatctgcgtt
     8101 tcgttttggt gcaatactcc ccgaaaaagg acgaattgcc gatttccacc aggcaatcgt
     8161 aaatcggcaa tggaggtaac aaaatcgatg tttaagagac aaactttaat tgcgtggcta
     8221 acgctcgtgg cgatcagcgt gatgccgttc acggcgctcg cgcaagcgac gcaaatccag
     8281 tatcacagca acaagtacag tattcaggac gacatcaaac tcggacgcca ggcggcgcag
     8341 gaagctgaaa gccagtttcc cttgctgcgc gatcaggaag tccaaagcta tgttgagagt
     8401 gtcggacgcc ggctcgttac ggccattcca tcgcaatttc aacatcccga atttcagtat
     8461 tacttcaaag ttgtaaacgc gagagacata aacgcgttcg cgcttccggg tggtccaatg
     8521 tacgtcaacc gcggcatgat cgaagcggcg cgcactgaag gtgagatggc gggcgtgatg
     8581 gcgcacgaga tcagtcacgt ggcgctacga cacggcacgg cgcaggcgag taagggacag
     8641 aagtacagca tcctcggcgg catcgccggc attgccggaa cgattcttgg tggaccaggc
     8701 gtcggccagc tagcccaggc gccgtttgcg gtttacctgc tgaagtttag tcgtgaatac
     8761 gaaactgaag ctgacattct tggtgcgcag atcatggccc aggctggtta c
//
LOCUS       DASSFQ010000265         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_868777, whole genome shotgun sequence.
ACCESSION   DASSFQ010000265 DASSFQ010000000
VERSION     DASSFQ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868777"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFH96_09530"
     CDS             <1..851
                     /locus_tag="VFH96_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HET6974253.1"
                     /translation="WTRKLSLLGPGVMGFFLNVMKSSYKAKAITEFRDELGLSDTGNP
                     MFEGQHSPTLVLALFSELFGQRQPDWPPQTEITGFCYYDGNHNVEIPVEFKHFLDNGA
                     PPIVFTLGSSAVWVARDFYEESIRAAKRLGRRAVLLIGDERNLPRALPEGIIALDYVP
                     YQTLLPRACAVVHHGGVGTTSHALLAGVPTLIVPFAFDQSDNAEHARKVGTSRTLYRD
                     KYLETRVADELHELLKRPSYARRAMEVGQRLKQENGPARAAELIEQVLSGERNRTEEA
                     VYATGD"
     gene            835..1599
                     /locus_tag="VFH96_09535"
     CDS             835..1599
                     /locus_tag="VFH96_09535"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET6974254.1"
                     /translation="MLLAIDAGNTNITLGLFRDQELIAQWRLVTERERGAGEYEADVL
                     DVFERAGIDRKEVKGIAIASVVPQLDHALRQIAASFAVEPLFVDHATDTGLKLLYQPP
                     SDLGVDRIVDGVAAMNKYGTPCIVVDFGTATTFNAISRDNEFLGGAISPGLFTCAEAL
                     FQRAAKLPRVEFVRPRLAIASSTVEAMQSGLYYGYAGLVDGMIEKMLEGMGPVSRIVA
                     TGGLAPLIAEASKYIDAIDETLTLDGLRIVYERVSI"
     gene            1596..2300
                     /locus_tag="VFH96_09540"
     CDS             1596..2300
                     /locus_tag="VFH96_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974255.1"
                     /translation="MNYFNYFTEIEDAFVRRRGKHLFLSPMDWALMETWKQQGIPLHI
                     VLRGVEKSFDSYEARPRKRSVKTLLYCQEEVESQYAEWVEAHVGSANASAETDSDKTP
                     FSFAAISEHLQRSRNALSELAASRKQEDDLCEALTRAIALLVDIEKDFASGATLDTRK
                     LEDSLTGLERMLNDSMLSVAGASTLNEFKKEVKDQLKPYRSQMDAAAYKQTYDNLLLK
                     RLREQFALPRLSLFFV"
     gene            2427..>2880
                     /locus_tag="VFH96_09545"
     CDS             2427..>2880
                     /locus_tag="VFH96_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET6974256.1"
                     /translation="MVELEIERILPGGLGLAHADGKTVFVSLAAPGDRVRVRVEREQG
                     NVLFASIQEILTPSPVRIEPPCPYFGRCGGCDFQQLTYEAQLAAKSEMIRDCLHRITR
                     LENIPDIFVTPSPNEWRYRLRAMWQIDRDERWIGYYERGSRRVCDVVDC"
BASE COUNT          622 a          736 c          865 g          657 t
ORIGIN      
        1 agtggaccag aaagctgagc ctgcttggac caggggtgat ggggttcttc ctgaacgtaa
       61 tgaagagctc ctacaaggcg aaggccatca ccgagttccg tgacgagctc ggactttcag
      121 acactggcaa tccgatgttc gaaggtcagc actcacccac actcgtgctg gccttgtttt
      181 cagagctgtt tggccaacga caaccagact ggcccccaca aaccgagatc acgggcttct
      241 gctactacga cgggaatcac aacgtcgaga ttccggtcga gttcaagcac tttctggaca
      301 acggcgcgcc accgatcgtt tttacgctgg gatcgtcggc cgtgtgggtc gcgcgtgatt
      361 tttatgagga aagtattcgc gccgcgaaac gtctcggtcg ccgagccgtg ttgctcatcg
      421 gtgacgagcg caacctgcca cgcgcccttc ccgaaggaat catcgcgctc gattacgtgc
      481 cgtatcaaac tttgctgccg cgcgcgtgtg cggtggtcca tcacggcggc gtcggcacga
      541 cttcgcatgc tttgctcgcc ggtgtcccaa cgctgatcgt tccgtttgct ttcgatcagt
      601 cagacaatgc ggaacatgcg cggaaggtcg gaacgagtcg cacgctgtac cgcgataagt
      661 atctcgagac gagagtcgcg gacgaactac acgagctgct caagcggccc tcgtacgcgc
      721 gccgcgcgat ggaagtcggg cagcggttga aacaggagaa cgggccggct cgcgctgcgg
      781 agcttatcga gcaggtgctg tcaggagagc gaaaccgcac agaggaagcc gtctatgcta
      841 ctggcgattg atgccgggaa tacaaacatc accctaggtc tgtttcgcga tcaggagttg
      901 atcgcgcagt ggcgattggt cactgagcgc gagcgaggcg ccggtgagta tgaagctgat
      961 gtgctcgatg tatttgagcg cgccggcatc gatcgcaaag aagtgaaggg aattgcgatc
     1021 gcgtcggtcg tgccgcagtt ggaccatgcg ctcaggcaaa tcgcggcgag tttcgcagtt
     1081 gagccgctgt ttgtagatca cgcgactgac actggtttga agctgcttta ccagccgccg
     1141 tcagatctcg gggtggatag aatcgtcgat ggcgtggcgg cgatgaacaa gtatggcacg
     1201 ccatgcatcg tcgtcgattt cggcaccgct actacgttca atgcgatcag tagagataac
     1261 gagtttcttg gcggcgcgat cagtcccgga ttgtttacgt gtgccgaggc tttgttccaa
     1321 cgcgccgcta aactgccgcg cgttgagttt gttcgtccgc gcctggcgat cgcgtcttcg
     1381 acagtcgagg cgatgcaatc cgggctttat tacggatatg caggactggt tgacggcatg
     1441 atcgagaaga tgctcgaggg catggggcct gtatcgcgaa tcgttgcgac cggcggcctc
     1501 gcgccactga tcgccgaagc ctcgaagtat atcgatgcca tagacgaaac tctcacgctt
     1561 gacggcttgc gtatcgtgta cgagagagta tccatttgaa ctacttcaac tacttcaccg
     1621 aaatcgaaga cgcattcgtc aggcgacgcg gcaagcatct gtttctgagc ccgatggatt
     1681 gggcgttgat ggagacgtgg aagcagcagg gaattccgct gcacatcgtg cttcgtggcg
     1741 tcgagaaatc gtttgactcg tatgaggctc gaccgcgcaa gcgctcggtg aagacgctgc
     1801 tttattgcca ggaagaggtt gagtcgcaat acgcggaatg ggttgaagcg cacgttggat
     1861 ctgcaaatgc ctcagcggaa actgactcgg acaagactcc attttcattt gccgcgatca
     1921 gtgaacactt acaacgcagt cgcaatgcgt tgtctgagct cgccgcgtcg agaaagcagg
     1981 aagacgatct ttgtgaggcg ttaacgcgcg cgatcgcttt gctcgtagat attgagaaag
     2041 attttgcgag tggcgcgacg ctcgacacgc gcaaactcga agactctttg accggtctcg
     2101 aacgaatgtt gaacgattcg atgctttcag ttgcaggcgc gagtacgttg aatgaattta
     2161 agaaagaagt gaaggatcag ctcaaaccct atcgttcgca aatggacgcc gcggcctaca
     2221 agcagactta cgataatttg ctgttgaagc gtttgcgcga gcagtttgcc ctcccgcgcc
     2281 tcagcctttt cttcgtttag ccacaaaagg cacagaagag tttttagcca caaaaaggca
     2341 caaaaagcac aaagagttta ttgaaaggga cggtgcccaa ttaataatca ttttgtgctt
     2401 tttgtgcctt tttgtggcta aatgaagtgg tggagttaga aatcgaaaga attctaccgg
     2461 gtgggctggg gttggcccat gctgatggga agactgtttt tgtttcgctc gctgcgccgg
     2521 gagatcgcgt gcgtgttcgg gtagagcgag agcaggggaa tgttttgttt gcatctattc
     2581 aagagatcct cacgccgtcg ccagtaagaa tcgagccgcc gtgcccgtac tttggccgtt
     2641 gcggtggttg tgattttcaa caactcactt atgaggcaca gctcgctgcg aagtcggaga
     2701 tgattcgcga ctgtctgcat cgcatcaccc gtctcgagaa tattcccgac atcttcgtca
     2761 cgccctcgcc caacgagtgg cgctatcgct tgcgcgcgat gtggcagatc gatcgggacg
     2821 agcgctggat tggttactac gagcgtggct cgcggcgtgt ttgtgacgtg gttgactgcg
//
LOCUS       DASSFQ010000266         4845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_274476, whole genome shotgun sequence.
ACCESSION   DASSFQ010000266 DASSFQ010000000
VERSION     DASSFQ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274476"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFH96_09550"
     CDS             <1..276
                     /locus_tag="VFH96_09550"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6974257.1"
                     /translation="LSKLVATLSDDNWRVRELAVWALREMKDARAVSALCNVLLSDVR
                     VEVRRGAAEALGEISSTDALPTLKQALNDKEPSVSAKAAWAISEIEG"
     gene            315..1760
                     /locus_tag="VFH96_09555"
     CDS             315..1760
                     /locus_tag="VFH96_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6974258.1"
                     /translation="MSSYKCVLLILFLTTAIFAQQKARTLKIKPYSFENEKKEKVDAE
                     FGTLLVPENRSNPESNLIELAFVRFKSTAKNPGPPIVYLAGGPGGSGIFTAKGSRFPL
                     FMALREVADVIAFDQRGTGYSKPNLGCFDQLSLPLDVAPTREAAIKELRENSRSCAQY
                     WRDIQRVDLTGYNTNESADDLEDLRKALGVNQISLWSISYGTHLAFATMRRHPTSIHR
                     AILAGTEGPDHTYKLPSNIQKHLEDLAAAIKADPEVGKEVPDFLGLMKSVFDRLDTEP
                     QTVEITDPRTKLKVKVIVNKFVMQYIVANNIGTTVTARFPALFYRASKGDFTNPAQVW
                     LDQSRSEIGSAMSYMMDCASGQTAARRDLIARDAKGTLLEDIFNFPFPDVCTEWNAPD
                     LGDEFRAPLKSDVPVLFISGTLDARTPISNAEEYRKGFSNSTHMIIEGAVHSDPLFLS
                     SPKIKEGMMEFLRGQTVTTTRIQATPIKFVL"
     gene            complement(1744..2373)
                     /locus_tag="VFH96_09560"
     CDS             complement(1744..2373)
                     /locus_tag="VFH96_09560"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6974259.1"
                     /translation="MTPRKLPREFYLRRDVLTVSRDLLGKLLVVLGPNRERVSGKIVE
                     VEAYRGPEDRASHAYGGRRTKRTETMYGAGGIAYVYFVYGMYYQFNVVCNVADIPHAI
                     LIRALEPVEGIDVMRARRHSHPDHNLTNGPGKLCIAMGIDRQLNAADLLGDEVWIEDF
                     ETISPRRIARGPRIGIDYAQEWIDKPWRFWIRDNRFVSKSVKSAAKEQT"
     gene            complement(2373..3149)
                     /locus_tag="VFH96_09565"
     CDS             complement(2373..3149)
                     /locus_tag="VFH96_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6974260.1"
                     /translation="MRLFSVLAMLCAAFAAGAINSIAGGGTLLTFPILIWLGLDPKVA
                     NGTSTVALWPGLFGGLFGYRKELEHSSKILFRLGSTSLIGGAIGGLLLVLTPSKVFAD
                     LVPFLILFATLLFMSNGIVNRRLRLGSIDEKPMGLWWTGAIIFQFFSAMYGGYFGAGN
                     GILMLAAMGVLGLNELHRANGIKNFLGICINAVAVLIFAGAGYVVWEDALLMACGALV
                     GGYFGANMAMRVGQKWVRRGIVLIGWIIFFVMIWKVRQHS"
     gene            3220..4611
                     /locus_tag="VFH96_09570"
     CDS             3220..4611
                     /locus_tag="VFH96_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6974261.1"
                     /translation="MRSAITALLFILAFQIAVAAQNGSQINWPRYQDMAVDLMQQYLR
                     VNTSNPPGNEIEAARFLKKIFDQHGIENEIFEFKPGRANIIARLKGNGSKRPIILLSH
                     TDVVTAEPASWEVDPFAGIIKNNSIYGRGALDMKGEGLLHLMTMIVLKREGPPLSRDV
                     IFIGTADEEVDDEGSLWMIANKADLFKNAEYLLTEGGDNLIEGDSVKVVGIDVAEKAP
                     FWLRLTATGLPGHGSRPVNDSATNRLIRAMNRILDWETPVKLLPAVEQFFKDIAPLQS
                     EPLRAHFLNIRESLKDPVFLKALTSQREFNFLVRNTISITMLSGSKQTNVIPNAATCN
                     LDVRLLPGESPEEFLKALTAVIADPSIKIENVNRFKQPNSSPIDTELFSLIARKTKEK
                     HAGAVVTTKMLSGYTESQLYRQLGITAYGWAPIYTTAEEDEGVHGNNERISVKNVREG
                     TRELYEIVRDISR"
BASE COUNT         1157 a         1357 c         1327 g         1004 t
ORIGIN      
        1 ctttccaaac tcgtcgctac cctttcagac gacaactggc gcgttcgtga acttgctgtc
       61 tgggcgttga gagagatgaa ggacgcgcgc gcggtcagtg cgctctgcaa cgttctgctc
      121 tcggatgttc gtgtcgaagt ccggcgtgga gccgcggaag cgctcggcga gatcagcagc
      181 accgacgccc ttcccactct gaaacaagcg ttaaatgaca aagagcccag cgtcagtgcg
      241 aaagcggcct gggccatctc cgaaatcgaa ggctaatccg cgttaaatcc gcgttcataa
      301 aggaggcatc gtcgatgtcc agctacaagt gtgtcctgtt aatacttttc ttaacgactg
      361 cgatcttcgc tcaacaaaaa gcgcgcacgc tcaaaatcaa accgtacagc ttcgagaacg
      421 agaagaaaga aaaagtcgac gcggaattcg gcacgctgct cgtccctgaa aaccgttcga
      481 acccggaaag caacctgatc gaactcgcct ttgtgcgttt caagagtacg gcgaagaatc
      541 cgggaccgcc gatcgtctac ctcgctggtg gtccaggcgg ctcgggcatc ttcactgcca
      601 aaggttcccg gtttcccttg ttcatggcgc tgcgcgaggt cgctgatgta atcgcctttg
      661 accagcgcgg caccggctat tcgaaaccga acctcggttg cttcgaccag ctctcgttgc
      721 cattagacgt ggcgccaact cgggaagctg cgataaagga gctgcgcgag aactcccgca
      781 gttgcgctca atactggcgt gacattcaac gcgttgacct caccggttac aacacaaacg
      841 agagcgccga cgatctcgaa gacttgcgca aagctctcgg tgtaaaccag atcagtctct
      901 ggtccatcag ctacggaaca cacctcgcat tcgcgacgat gagacggcat ccgacgagca
      961 ttcatcgcgc gatcctcgcc ggcacggaag gtccggacca cacctacaag cttccgagca
     1021 acattcagaa acacctcgaa gatctcgcgg ctgcgatcaa agccgatccg gaagttggaa
     1081 aagaagttcc tgattttctc ggattgatga agtcagtctt cgatcgcctc gacactgaac
     1141 cccaaacagt tgagatcacc gatccacgga cgaagctcaa ggtcaaagtc atcgtgaaca
     1201 agtttgtgat gcagtacatc gtcgcaaaca acatcggcac gacggtgact gcgaggtttc
     1261 ccgcgctttt ctatcgcgca tcgaaaggcg atttcacgaa cccggcgcag gtttggctcg
     1321 atcaatcgcg cagcgagatc gggtcggcta tgtcttacat gatggactgt gcttcaggac
     1381 agactgcggc gcgccgtgat ctgattgccc gtgacgcgaa ggggacgctg ctcgaagaca
     1441 tcttcaactt tccgtttccg gatgtttgca cggaatggaa cgcgccggat ctcggtgatg
     1501 agtttcgggc gccgctgaaa tcagatgtgc ccgtgttgtt catctcggga acactcgacg
     1561 cgcgcacgcc aatcagcaac gcggaggagt atcgaaaggg attctcaaac agcacgcaca
     1621 tgatcattga aggtgcggtg cattccgatc cgttgtttct atcgtcgccg aagattaaag
     1681 aggggatgat ggagtttttg agaggacaga ctgtaacgac gaccaggatt caagcaacgc
     1741 caatcaagtt tgttctttag ccgcggattt aacggatttg ctgacgaacc gattatcgcg
     1801 gatccagaag cgccacggtt tgtcgatcca ttcctgcgcg tagtcgatgc cgatgcgtgg
     1861 accgcgagcg atgcggcgcg gtgagatcgt ttcgaagtct tcaatccaca cttcatcgcc
     1921 gagcagatcc gcagcgttga gttgtcgatc gatgcccata gcgatgcaga gttttcctgg
     1981 gccattcgtg aggttgtgat cgggatgcga gtgccggcgc gctcgcatca cgtcaattcc
     2041 ttcgacgggt tctagagcac gaataagaat cgcgtgggga atatcagcaa cattgcagac
     2101 cacattgaat tgatagtaca tgccgtaaac gaagtagacg taagcaatgc cccccgctcc
     2161 gtacatcgtc tctgttcgct tcgtgcggcg tccaccgtaa gcatgtgacg ccctgtcttc
     2221 aggaccacga taagcctcga cctccacgat ctttcccgac acgcgttcgc ggttggggcc
     2281 aagcacaacc agcaacttgc ctaacagatc acgagaaacc gtgagcacat ctcgtcgaag
     2341 gtagaactcg cgcgggagct ttcgtggtgt cattaagagt gttggcgcac tttccagatc
     2401 atcacgaaga atattatcca cccgatcagc acgattccgc gtcgcaccca tttctgtccg
     2461 acacgcatcg ccatgttcgc accgaaatag ccgccgacga gcgcgccgca cgccatcagg
     2521 agcgcatctt cccaaacaac atatcccgcg cctgcgaaga tcagcacagc caccgcgttg
     2581 atgcagatgc cgagaaagtt tttgatgccg ttggcgcgat gcagctcgtt gagaccgaga
     2641 acacccatcg ccgcgagcat gaggatgccg ttgccggcgc cgaagtaccc gccatacatc
     2701 gcggagaaga attgaaagat gatcgcgccc gtccaccaga gacccatcgg cttctcgtca
     2761 atcgacccga gtcgcaggcg cctgttgacg atcccgttcg acatgaagag cagggtcgcg
     2821 aacaggatca ggaacgggac gaggtcggcg aagactttcg acggcgtgag aacgagcaat
     2881 agtccgccta tcgcgcctcc gatgagactc gttgaaccga gtcgaaacag gatcttggaa
     2941 ctgtgttcga gctctttgcg ataaccgaac aggccgccaa agagaccggg ccagagtgcg
     3001 accgtgctgg tcccattggc gactttcgga tcgaggccga gccagatcag aatgggaaag
     3061 gtcagcagcg tccctccgcc ggcaatggaa ttgatcgcgc ccgctgcgaa cgcggcgcat
     3121 aacatggcaa gcacggagaa gagacgcatc gtttgagtta gcgcatgtta atataaatcg
     3181 tgcaaatcct gtaatcctgt ctggaagagc ttcgactaaa tgagatctgc gataacagcg
     3241 ctacttttca tccttgcatt tcagattgcc gttgcggcgc agaatggtag tcaaatcaat
     3301 tggccgcgtt accaggacat ggcggttgat ctgatgcagc agtacctgcg cgtgaataca
     3361 tcaaatccgc ccggcaacga gatcgaagct gcgaggtttt tgaagaagat cttcgatcag
     3421 cacggtattg aaaacgagat cttcgagttc aaacccggtc gtgcaaacat catcgctcgt
     3481 ctcaaaggta acggctcgaa gcgtccaatc attctgctga gtcacactga cgtcgtgaca
     3541 gccgaacctg cctcgtggga agtcgatcct ttcgcaggca tcatcaagaa caattcgatc
     3601 tacggtcgtg gtgcgctcga catgaaaggc gagggactgc tccacctcat gacgatgatc
     3661 gtgctcaagc gcgaaggacc accgttgtca cgcgacgtga tctttatcgg caccgctgac
     3721 gaagaggttg acgacgaagg ctcgctctgg atgatcgcga acaaagcaga cctcttcaaa
     3781 aacgctgagt acctgctgac cgaaggcggt gacaatctga tcgaaggcga ttcggtaaag
     3841 gtggtgggaa tcgatgtcgc tgagaaggcg ccgttctggt tacgactcac cgccaccgga
     3901 ctgccgggac acggctcgcg ccccgtcaat gattccgcga cgaaccgtct gattcgcgcg
     3961 atgaatcgca ttctcgattg ggagacgccg gtaaagcttc tgcctgcggt cgagcagttc
     4021 ttcaaagaca ttgcaccatt gcagtcagag ccgctgcgcg cgcatttcct gaacattcgt
     4081 gagtcgttga aggaccctgt ttttctgaag gcgctgacga gtcaacgcga gttcaacttt
     4141 ctcgtgcgca atacgatctc gatcacgatg ctgagtggca gcaagcaaac taacgttatc
     4201 cccaatgctg cgacgtgcaa tctcgacgtt cgactgctgc cgggcgagtc gccggaagag
     4261 tttctcaagg cgctgacggc cgtgatcgca gatccctcga tcaagattga gaacgtcaat
     4321 cgcttcaaac agccgaacag ttccccgatc gacactgagt tgttttctct catcgcgcgc
     4381 aagacgaaag agaagcacgc gggcgcagtt gtcaccacaa agatgctcag cggttatact
     4441 gaaagccagc tctaccggca gctcggcata accgcttacg gatgggcgcc gatttacacg
     4501 acggccgaag aagacgaagg cgtgcacggc aacaacgagc gcatctctgt gaagaacgtg
     4561 cgcgagggca cgcgcgagct gtacgaaatc gtcagagaca tctcccgtta gccaagcccc
     4621 caatttcttc tctgcgctct ctgcgaatct cagcgttctc tgcgttgagg cgccatttaa
     4681 cgcagagaac gctgagatac gcagagactg cgcagagaag aaaataaata caatcagggg
     4741 gcttggttaa cgggagatgt ctctgacgat ttcgtacagc tcgcgcgtgc cctcacgcac
     4801 gtttttcacc gagatgcgct cgttgttgcc gtgtacgcct tcgtc
//
LOCUS       DASSFQ010000267        30428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2071289, whole genome shotgun sequence.
ACCESSION   DASSFQ010000267 DASSFQ010000000
VERSION     DASSFQ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 30428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30428
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071289"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFH96_09575"
     CDS             complement(<1..467)
                     /locus_tag="VFH96_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HET6974262.1"
                     /translation="MAQPYVGEIRMFAGNFPPVGWMFCDGQLLPISENETLFQLIGTM
                     YGGDGQSTFQLPDLQSRIPLHFGTGPDGITYQQAEAAGTESVTLTTQQIPIHTHAQLC
                     NSGAGQGVADPVNNIASKTDLAQYSSAGATVAMGTPNFVSDIAGGSQPHENCQP"
     gene            875..1255
                     /locus_tag="VFH96_09580"
     CDS             875..1255
                     /locus_tag="VFH96_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974263.1"
                     /translation="MALAAGAPLALTEKAFGIGTTKSTAIGLNMAAFKSQLGTNFLIN
                     HQASKVKFTLVDVTDFASRKQSAAGKEGFSLLFRGPKESTLKQDTYLIEHEQLGMFSF
                     LIVPVGTQDTRAPYYEAVINRLHS"
     gene            complement(1277..1765)
                     /locus_tag="VFH96_09585"
     CDS             complement(1277..1765)
                     /locus_tag="VFH96_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6974264.1"
                     /translation="MSTNVTLRPVGPDDRDFLIEVYGSTRAEEMALVPWTDEQRDAFI
                     QHQFTAQTDHYTQTYPAANHDIILSDDRPVGRLYVARLDHEIRIVDITLLPAERNAGI
                     GSYLIKQLLDEANRSGKITRIFVEEFNPSRSLFERLGFSPSEQHGIHLLLQCNPSQHL
                     TP"
     gene            complement(1762..2073)
                     /locus_tag="VFH96_09590"
     CDS             complement(1762..2073)
                     /locus_tag="VFH96_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974265.1"
                     /translation="MEASLTHEEFSKHANTKFQVQLDQPPPVELDLVEVSELKIYPQQ
                     EEFTILFRGPREAFLDQGVRFLKHDQMGEFELFIVPVSQDAEGFYYEAVFNRIREQSG
                     Q"
     gene            complement(2074..3729)
                     /locus_tag="VFH96_09595"
     CDS             complement(2074..3729)
                     /locus_tag="VFH96_09595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6974266.1"
                     /translation="MPTYGINPYISFVESRVFPGVVQHAVFHRLTGVIAEPDERVRSL
                     LLAAKSGTPLLINEADLQMRQLVQMEFLIPKGHDPLELLLDQYVTRPIQNPAIAYRSK
                     TGEWILVRTSMLQAVYSRRLGELPEIVEEKLSPVAADILLKADGTKTLRQVYNELQVE
                     DSEFRSAIDFLTSQERQLIKFTVRPEELNEPFSLVNIIPRNLYHSERKDGDGITDFHL
                     SGIEDAAWEFDQIEPTVNHGFRFPHEALGGLNYGSRFCLATLRTEVVPLLNNSNALEV
                     LEVGGGMGTFARAFLQQAASVNGTSINYHILDLSPALMASQRKILAELLPESRHFQQD
                     ATEFDLPDHTFDLIISNEVIADFPVASLQRSNDGTWQGEGAYYLDKYNLTDKDAPDSF
                     VVNAGVFRFLERAWKHLTPGGTLILSEYGSEQHYPAASFNLNHDEFTIHFGHVVACAA
                     KVGFQCRLLTLKEFLNFDTDVLVLDGREEHLLCLNHVLKDYGEVLPYAVISKSDFEQR
                     CRSVVEQTGLIGYSFSPLHKGFHFGPNIRDFLVLIMHKPEEEI"
     gene            complement(3992..4474)
                     /locus_tag="VFH96_09600"
     CDS             complement(3992..4474)
                     /locus_tag="VFH96_09600"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6974267.1"
                     /translation="MVQNDETNLSLREVTAEDEPFLLEVYASTRLEELEGFGWSDEQK
                     QAFIKMQFLARERVYPRVDNRIILFEGRPVGRILVDRSDTTILLRDIALLTEFRNARL
                     GSRLVQDLMNEATAAGKPIQLHVLASSPAVRFYERLGFRRTGGDNAYLEMRWVPADVS
                     "
     gene            complement(4520..6430)
                     /locus_tag="VFH96_09605"
     CDS             complement(4520..6430)
                     /locus_tag="VFH96_09605"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6974268.1"
                     /translation="MLLECPAMPPRLAAISGKLKGAIFALNEESLIIGRETSANLCIA
                     DASVSRRHTQIEKHETGFVITDLESLNGTFVNDLPIRSRLLEHGDRVRIGDSQFLFLT
                     HEGDATSKSSDVTIDEAHVVSGSTVQIRFDDAIYQMTRDLSALMKISTEINSIRGLDN
                     LLERLLDLLFEVVPAERGAILLTDESSFETSLVFGLDRVYGKDAAVNVSRTIVQQVLR
                     DGVALLANDAPSESGLVTDSLIEARTHSVMCVPLILFDRKLGVIYLDSTVTRDHFNRE
                     HLQLVTAIAGIAAVAIENARQFEWLETENERLRTDVNIEHNMVGESAAMQRVYHFISK
                     VAPTEATVLISGESGTGKELAARAIHRNSKRAQKPFMAVNCAALNESLLESELFGHEK
                     GSFTGAFAQKKGRLEIADGGSVFLDEIGELTPPLQVKLLRVLQEREFERVGGTVTINV
                     DLRVIAATNKNLEEAIEMGQFRQDLYYRLNVVSLEMPALRERREDIMLLANYFANKFG
                     TRSNRKLKGFSEEARARLTSHDWPGNVRELENAIERAVVLGTTDLILAEDLPEVLLER
                     DVNVVNLNSYHGAVAQAKKQIILNAMEQTSGNFSDAAKLLGVHPNYLQRLVGNLNLRE
                     QLKRFLAADGRR"
     gene            complement(6490..8136)
                     /locus_tag="VFH96_09610"
     CDS             complement(6490..8136)
                     /locus_tag="VFH96_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HET6974269.1"
                     /translation="MKTHKSPLIYSLSVLIMLLALVLVGRSFAPERVQITILGTTDLH
                     GNINPIDYYTNKPDNRGIAKVATLIKRIRKEQPNVVLIDSGDTIQGSPLESFHGRKNN
                     APPDPMMLVMNSLNYDSMTVGNHEYNFGLKVLEKARGEAKFPWLSANTYDTAKQQTHY
                     KPYIIKEVAGVKIGILGLTTPGIPNWDNPPNYAGLEFHEPLVEARKWVPILRDQEKAD
                     VVVIAMHMGLEEDLSTGVMSPGQVLHENEAILIAKEVPGVDVIFMGHTHREVPSLYIN
                     GVLLTQANYWGRHLARADLYLQKAGTGWRMYAKSARTMPTDDRVEPDPDVVKIAEPYD
                     RETQAWLSREIGESPEELTARDARFRDTAILDLIQKVQLDAGKADVSMVASFNPAARI
                     AKGPVSVRDIAGLYVYENTLVVLEVTGQQLKDALEHSAKYFKEYVPGKSASDLVDEKI
                     PAYNFDIAEGVTYDLDISKPIGQRIQNLRFHGQPLSLTKKLRLATNNYRVNGGGGYDM
                     YKNAPVVYRSSEEIRELIIDWVEKNKTVPTKPNNNWRILP"
     gene            8239..9336
                     /locus_tag="VFH96_09615"
     CDS             8239..9336
                     /locus_tag="VFH96_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974270.1"
                     /translation="MDNLVTSNIRQRPIRTLVSVAGVALGVALVMLFTGLARGMSNEL
                     QRRATNVRAELIFMRPGAVQLTATTANLDTRYVDRLKQIEGIEEALPVIRHFSQGTKG
                     IGIEQIDGVDWDAYARMNGINMVAGNGPQSLDEVVIDEVKAEQNNLHVGDRMKPLGTQ
                     EYRITGIYSPESGARVKMSLAAMQKALEAEGKCTFIFLKLRNSDQLKEVAQRIDRELP
                     GNTIQPTREVFTSFEKSIPLLGVFLRVLVVLAAVVSSLVVMLAMYTTITERTREIGIL
                     KAMGASRGYIVGIIEKEAILISLIGLVAGFVVSLVAGYLIHKAYGLVFEYSWQWAVVA
                     GTIGIVGGILGALYPAWRASNLDPVNALAYE"
     gene            complement(9327..10292)
                     /locus_tag="VFH96_09620"
     CDS             complement(9327..10292)
                     /locus_tag="VFH96_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET6974271.1"
                     /translation="MGLLLFAYFVSRAGVGEILSNIQRLGAGFVLILAVSSIRYVVRA
                     LAWTRCIEPPHRLPFRDAFAARLMGDALGNIIPFVSVAVSEPSKAVFVKDRVPLMVGL
                     SALAIENIFYALTVAIFIFSGTATLLLTFSLPKGLRYASIGALVTTLVIIPLVYLLIH
                     WQARFLSGALGFLSRRGVLTKVVGKLIPRAQSLEDRVYGFYATSRRSFLSIFLLDMSF
                     HLAGVLEVFITLSFISPVAPTLTQAFILESVNRIINVAFKFIPLRAGVDEGGTGQVSK
                     VLGFARGIGETLAIVRKGRDIFWSAIGLLLIWKNNRSYSSYRTYS"
     gene            10521..11702
                     /locus_tag="VFH96_09625"
     CDS             10521..11702
                     /locus_tag="VFH96_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6974272.1"
                     /translation="MSQSQSAIATPAPSPLTTLTEDETMFRSSVREFAEGELRPRVEH
                     MDEVAKLDPAIIKQCFDLGLMGIETPEEFGGAGANFFTAILAVEELSRVDASVGVFVD
                     VQNTLVNNAFIRWGTPEQKKKYLTRLASESVGAYALSEPGSGSDAFAMQTRAVEQDDH
                     YVLNGRKLWITNGIEADIFVLFANANPEAGYRGITAFVVEKSFPGFGIGKKENKLGIR
                     ASSTVELILEDCKVPKENVLGEPGKGYKISIETLNEGRIGIGAQMIGIATGALEAALG
                     YTAERQQFGKSINQFQGVQFQLAEMATELEAARLMVYNAARMKDAGVNFVKEAAMAKL
                     YSSRVAERVSSKAIELYGGYGYVKDYPVEKYWRDSKIGAIYEGTSNMQLQTIAKLIIG
                     K"
     gene            11809..13962
                     /locus_tag="VFH96_09630"
     CDS             11809..13962
                     /locus_tag="VFH96_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HET6974273.1"
                     /translation="MNTDTKVLKLVRKSESTHTTATELPDHKLLIEMYRTMYLSRRLD
                     DKEIQLKGQNRIFFQISGAGHEAVLVAAGVVLKPGYDWFYPYYRDRALCLQLGMTPLE
                     MFLSAVGAEADPNSHGRQMPSHWGHKKLNIVSQSSPTGTQLLQAVGCAEASYRFELIP
                     ELQKRVSGFEDDEVTYVSVGDGTTSEGEFWEALNTASNLKLPVLFLIEDNGYAISVPV
                     EVQTAGGDVSRLVENFPSLYLQRCDGTDPIESLETMRRAVEYCRQRKGPALVHAKVIR
                     PYSHSLSDDEKLYRPDEERASDAERDPVKRFGALLIDEGVIDQDGLQQIKDEVDALVN
                     EAADMALTSPQPARETATKYIFSPDVDPASKEFDTEDSVQLSGNPGTMVDLINRCLDT
                     EMARDPRIVVFGEDVADCSRDQYLEAVKGKGGVFKVTANLQRKHGSARVFNSPLAEAN
                     IVGRAIGMATRGLKPVVEIQFFDYIWPAMHQIRNELALLRWRSGNEFKAPVVMRVPVG
                     GYLKGGAIYHSQSGVSTFTQIPGLRVIYPSNALDANGLLRTAIRCDDPVLFLEHKHLY
                     RQAYNKSAYPNDEFMIPFGKAKKVREGTDLSIITYGALVQRSLVAAKQMEQEGISVEI
                     IDLRSLVPFDWNAIAETVKKTNKVIVAHEDSLSWGYGSEIAARITDELFEYLDAPVKR
                     VCALDTFVAYAPQLEDAILPQASDVAKAIAELKSY"
     gene            14190..15197
                     /locus_tag="VFH96_09635"
     CDS             14190..15197
                     /locus_tag="VFH96_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974274.1"
                     /translation="MRKHFSIVWGAMVLTLALAAIALGQTTTVTKAVQNPDGTYTIIE
                     YPVKKEVMLNLTPVNVAGAKGMATILRDDDGTRIKLNLTGVPADMTAMTLYAVDDTGT
                     VTALGPVAITNGTGTLAATTPLSKFMLFASPEPALTAYDPNTKVFFRSAVPDGFTVIP
                     VARTGDEKVAAVGAPVAATAQTTTTTTPGAVVSSPSGTTVVIPMTETKTTYTAPLLNI
                     PAYKTGDDTKIKVDFTGALTGARANVFITPRKDGPTEVKMRFHELKEAPAGQSFILWA
                     VSPDNKFIRLGQVVNTGGRNEAEIRSEVPLPDFGLIVTMESAVGELPVGPAIGTIHIV
                     P"
     gene            15448..16593
                     /locus_tag="VFH96_09640"
     CDS             15448..16593
                     /locus_tag="VFH96_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6974275.1"
                     /translation="MIKRSYYPRLFPLALILLVASFGRASVHADETLPNLVKRVKPAV
                     VAIATYDANGEALMTGSGFFLRPGQVVTNLHVIRGAVRAEIKTLDGKGKIFPVNGTLA
                     VDEEGDLALLSVEMPVANAIRSTELAKDLPDEGETIFVIGNPLKLEGSVSDGIVSAVR
                     EVPNSYRIIQITAPISHGNSGSPVFNLKGQVLGVVTIKVTNGQNINLAIAAARVAELT
                     AGKLQPLSELALKGRGDVAESLYRSGLDSLWLGNYDNAVGYFENAANKNPKRADTWVQ
                     VGYCKVKQGKNGEAIRAYQQALQLKPNDPEIYNKLGDAYYYSGRLREAIDSYSEAARL
                     RPDWAETFYNLAIAYSESGNPSMAAQQARILQRLDPKLYEKYLRETM"
     gene            complement(16643..18079)
                     /locus_tag="VFH96_09645"
     CDS             complement(16643..18079)
                     /locus_tag="VFH96_09645"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6974276.1"
                     /translation="MSQFINQSEQESGMSFRAKLTRRLISSALLVAMMFVASTDYSQA
                     TQPQQTKPQSQETVHSVVAKQAALVSEFEVNGLKVLLKRREGSLTVAAGLFIRGGAAN
                     INAENAGIETLMLSAATEASANFPREKMRSELSRMGTVIGSSSNNDYSVLSLASTRMH
                     FDRSWEMFADVALRPSFTKEDVALVQQRQVVSLSDDTDNPDVYLQKLQERVAYAGHPY
                     VNNTNGTVETVGKLTPDDLRAYHTKLMETSRLLLVIVGDLNPNDVRGLVAASFGKLPR
                     GNYMAKPIPQLAFDKSTVEVTPRELPTNYIQGLFTAPSLTSPDIYAMRIASSLLRDRV
                     FEEVRVKRNLSYAPDAFLRTQAANVGGLYVTAVDANQSIRVMLSEIARLQTEPVDADD
                     IQAVVAQYLTTYYLGQETNAAQAGELAQYELIGGGWRNSVNFLEKLTAVTPADIQRVS
                     QKYMKNIRFVVLGNPKSVDTGVFTGQAG"
     gene            complement(18095..19519)
                     /locus_tag="VFH96_09650"
     CDS             complement(18095..19519)
                     /locus_tag="VFH96_09650"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6974277.1"
                     /translation="MKRSSLVFLLLLTLLTPARAQQRSTKPTTTTAPATAATPARLPE
                     ANLISKVLANGLEIIVLEDHSIPLVTIELAVRNGSYTEPPELNGLSHLYEHMFFKQNR
                     AIANAEDYVRAIGSKGIAYNGTTREEVVDYYFTTTSPNLRTAMQFMKDSVRYPLFDER
                     EFGREKQVVIGEIDRNESNPFFYLAREMNDRLFYKYPSRKNALGNRETVGGATTDMMR
                     LIQGRYYVPNNSALVLTGDVNPTEAFAMAQELYGDWPKREKDPFVEFPLVDHPPLTKS
                     EGAVIQQPVQNVIINIGWHGPSIGKDNPATYAADVFSFILRQPNSRFQRNLVDTGLVT
                     GVDLSYYTQRNVGPIALIAQTTPDKARAAIKAMYEEVAHFNDKDYYTDEQLESAKALL
                     EADDLYSREKLSDYTHTLSFWWASTGLEYFRGYLPRLRATSRADISRYITTYIQGKPH
                     VGLALMSSDAQAKVKLTPEELIGQ"
     gene            complement(19651..21270)
                     /locus_tag="VFH96_09655"
     CDS             complement(19651..21270)
                     /locus_tag="VFH96_09655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6974278.1"
                     /translation="MDVPQLIAGRFRIERQIGTGGMGAVYLATHLDLERPVAVKIIRP
                     EFASDADVSDRFLREARTMAKLRHPNAAMIFDAGNLPDGRHYIVMEFVEGETLSQALA
                     RQGRFSFTQAIDIATQICDVLEEAHRLGIIHRDLKPSNILLGKRGVCVLDFGVAKVLA
                     SSAESTATHASTGSGQLIGTPRYMSPEQCLGQRVGARSDLYSLGVLLYEMLAGRPPFV
                     DPLQSALLVKQATAAPPPLPRLRQDIPRSLALAVHSLLAKRPEDRPRTAAMAKMLLER
                     SLLRPEGPVAPADPMSSMVAAADAGRGLLFRVGAPTILVTIFSALLVWGYTGGQSATE
                     PRIPDSALPVAASQSHFTPASWRDSSTAPSGGVTTPRENPKPVEQLTLDQARKIAARF
                     AKGSVGDVEIVDTDSGQAIVAINNQRKAGTTNFLLLQKQNGKFRLSAQGRLDAEGFSH
                     ASWSSDLVDADEDGFEELIFSGKDSSESRSLRRLVLFVPNDKRTYSMQMTGETTPRGT
                     PRIQWLSNAAGSDAAAYRTALRQKARSMIRK"
     gene            complement(21413..22270)
                     /locus_tag="VFH96_09660"
     CDS             complement(21413..22270)
                     /locus_tag="VFH96_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974279.1"
                     /translation="MPTEPMTYNSARDGNDPNESTPFYEALAKALKKRKIKIEAVCPP
                     TDPVSRRILEEYGAIYVANKKVTPPPVCIFTTEEQVTKFQDDAGFEAELIGYDEIELQ
                     PEALKSLNKARKDAQKEGLDITPRDGAEAARRNYEDSVRLWNSRFDPALDYWLSQGRL
                     TEEQVTRMRGLPLRQQVAEVLELEKTGIYFSKDLSKSILYSIAAPGASQHIAMLAFDV
                     NEFDNPRVREIMAKHGWFQTVLSDLPHFTYLGLKEKDLPKNGLRSLEINGQMFWIPNV
                     PGIHPGLDK"
     gene            22462..22803
                     /locus_tag="VFH96_09665"
     CDS             22462..22803
                     /locus_tag="VFH96_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974280.1"
                     /translation="MKSADLFTELTAMYRKHGWQLRSVLLRPETRVELQLPENLPVKE
                     ATFDALWFSRPSHDDREAWELRLLAQTQYALFETFEADETEEEREDLRLEMEARLREY
                     LATDNTDKKSD"
     gene            complement(22838..23737)
                     /locus_tag="VFH96_09670"
     CDS             complement(22838..23737)
                     /locus_tag="VFH96_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6974281.1"
                     /translation="MSQVAETICSNCHSTMPAELRFCRNCGFRLTGTTGGYTGPAAVA
                     DSAALSKKKRRLSGMSWIFIGLLVFFVGAAAFTALVAPLAKHGNGIVSRTPVNKSYIG
                     IDGFNNSDQGVVFEAVTTPGGPADQAGLVGGDVILSFDGQPIKDEDQIQELMEKTPIG
                     KTVDIEYIRDGQKKTAKLTTISLDDNRRLTREFDHRPEGRAQFGYEDGDAERVQVPGT
                     NTYGVKLGTILRSRPADLAGVKEGDIITAFDGVPIRTSEEFLMRVRRALPYSTVKLSI
                     VRPGQNETDPVEKLEIPVKMGKQ"
     gene            23887..24111
                     /locus_tag="VFH96_09675"
     CDS             23887..24111
                     /locus_tag="VFH96_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974282.1"
                     /translation="MEITRALAALQPLYGKDNVEIITTDGRRVRGTVRSMKTTQALTT
                     PSVKIEIANGQIVKIPFPTIAEIKDHNVST"
     gene            24153..24734
                     /locus_tag="VFH96_09680"
     CDS             24153..24734
                     /locus_tag="VFH96_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974283.1"
                     /translation="MPLPLSIDDFGTRIASVWSELRPATRGLIERALQAASNGTQTPR
                     NFQYDARADLELSRFLAALDAEKNGKLKSIADTCAAVLLEKTESAEVFAQLVRRAETQ
                     RDYRRIDILADALTARFPPSEICELARSEDTIVRELANEALARCPVSVLTALLNDPVD
                     AETARAALRRQVIEYGSEEARQISNALDQADEM"
     gene            complement(24876..25721)
                     /gene="ispH"
                     /locus_tag="VFH96_09685"
     CDS             complement(24876..25721)
                     /gene="ispH"
                     /locus_tag="VFH96_09685"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET6974284.1"
                     /translation="MEVLLAEEFGFCFGVERAVEMVEEVVEEGARPIRSLGPLIHNAQ
                     EMERLGARGVSTIDSPDEADADTIAVIRAHGVTPQVQHELEQRAAKVIDATCPFVTRV
                     QHLAERAARQGRDVVVAGNPDHPEMIGVVGYAPNNTYVVRDANEVAKLPKLRSPLVVS
                     QTTIKLQTFLDVAEAVREKADTEPQVVNTICSATRDRQDAARALAGQVDAFYVIGGRH
                     SSNSVKLLAVCEEQCAKSFLIETPAEINPADLQGVKKVGVTAGASTPNWLIDQVVERL
                     HSISK"
     gene            complement(25723..25986)
                     /locus_tag="VFH96_09690"
     CDS             complement(25723..25986)
                     /locus_tag="VFH96_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974285.1"
                     /translation="MAEGRDPIRDVAEAVLGGLRQIEDCSYAIMPKNIAHSVAGVKKA
                     LLGHLTNALRWEMEWIDDRVAGGDKLREEWREKARQRTSQAAQ"
     gene            complement(26051..27352)
                     /locus_tag="VFH96_09695"
     CDS             complement(26051..27352)
                     /locus_tag="VFH96_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6974286.1"
                     /translation="MKSRRDFLSLAGKGLGLAALTTPTIGALLKEVEAATRSVAHLTP
                     EQVATDEDYWSIIQNSFTVTRGIVNLNNGGVSPSPRIVTEALVRYTWQQEDATAYTMW
                     QLLEPQSETIRTGLAEMFGVDREEIAITRNASESLEILLMGMDFKPGDEILTTTQDYP
                     RMLTTLRQRERREGLVLKMVQIPIPPKNLNEITAAFEKGITSRTKLILVSHQVNITGQ
                     ITPVKAICDLARAKGIETIVDGAHSYAQFDFKQKDLGCDYFGTSLHKWLYAPKGTGLL
                     YVKRERVEKLWPLMAAESKQANDIRKFEEIGTHSAAPRLAIGEALLFHNGIGGKRKEA
                     RLRYLSRYWMNRLKDVPKVSFNTSFDPDQSCAIANVNIAGIDPTAIGSYLFNKHRVFT
                     TPIVHEEFKGIRITPNVYTTLGELDRFCNLMESIARNGLPT"
     gene            complement(27458..28396)
                     /locus_tag="VFH96_09700"
     CDS             complement(27458..28396)
                     /locus_tag="VFH96_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6974287.1"
                     /translation="MKTAILSLVLGSLALSSTLLAQEPNPPVAKPAGPTVRLSLIVTD
                     SKDKSLDAISKDDLRILDDTVEQTILSVEADVRPVDLGIAIDASGSFLRYLEPVIKGA
                     KLMVVNRNPKDEIFLERFISSDKIETLQDFTSDQKLLTSAVESIYVEGGQSAVIDAVY
                     MGVEHIAGHNRNSDRRKALVVFTDGEDRNSYYNVEKLLAFLHKETVQIFVVGLILDLQ
                     DKPTNITRAGNRERAIKLLTTLAEETGGRVFFPRDDSELGHAIAQIVHDLDAQFRITY
                     QSSNTVKKNFRKVEVKVISLAGENRKAIVPRGYETQ"
     gene            complement(28404..30086)
                     /locus_tag="VFH96_09705"
     CDS             complement(28404..30086)
                     /locus_tag="VFH96_09705"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6974288.1"
                     /translation="MKLFLIAASLLFVSFIPIQQSTPADIVFKNGNVYTANDKTPRAQ
                     AIAVKADKIVFVGTNAAAQKFVGPSTRVVDLKGSTVLPGFTDAHQHLSGVGQREMTLN
                     LEGTTSLDDLLAKVKARVDQAKPGEWVTGRGWIETHWQPPVFPTRWDLDKVSPNNPVI
                     LGRADGHGAVANSAALKLANVDKNTPNPFGGEISKDKQSGEPNGMLLDAAQGLVRRRV
                     PPTTREDDEREVVLGAKRDVELGWTQVQDAGGDYGDVDIFKKLYAAGTLKLRIYKAVY
                     GPGPQANRLLNEGAIIGEFGNRLTVRTIKVVSDGALGSRGAALLGPYSDAPDTSGFLT
                     VKAEDLRPMLIDALKKGIQVETHAIGDRANRFILDEYETALKAVPVSERKVVEPRWRV
                     EHAQIVNPADIPRFAKLGIIPSMQPSHAIGDLFFAPSRLGIPRLAGAYAWESFIKSGV
                     VVPGGSDAPVERGEPMIEFYAAVARKDQKGFSGEGWHPEEAVTREQALKMFTIWPAYA
                     AFEDKLRGTIEVGKLADLTILSADIMTIPAPEILKTRCVMTVINGEIVHEAT"
     gene            complement(30135..>30428)
                     /locus_tag="VFH96_09710"
     CDS             complement(30135..>30428)
                     /locus_tag="VFH96_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET6974289.1"
                     /translation="SRYGCALVGMTIGADGAVVYHEGKFIESPGFAAPGGCRDTTGAG
                     DAFHAGFLYGFLTGENLETSLKLGNAVAAMKCSALGARTALPTKSELDKFLRA"
BASE COUNT         6751 a         8362 c         8277 g         7038 t
ORIGIN      
        1 ggctggcaat tttcgtgtgg ctgggaaccg ccggcaatat cgctcacaaa attcggggtt
       61 cccatggcga cagtcgcgcc tgcactgctg tattgggcca agtccgtttt cgaagcaata
      121 ttgttgaccg gatcggcaac accttggcca gcgccgctgt tacacagctg ggcatgggta
      181 tgaatcggaa tctgttgcgt tgtaagtgtt actgactctg tgccagccgc ttccgcctgt
      241 tggtaggtaa taccatcagg accagtaccg aagtgcagcg gaatccggct ctgaagatcg
      301 ggcaattgaa atgtactttg tccatctccg ccatacatcg taccgatcaa ctgaaacagg
      361 gtctcgttct cactaatcgg caagagctgt ccatcacaaa acatccagcc aaccggagga
      421 aagttaccgg caaacattct tatttcgcct acataaggtt gggccatcga atcgtctcct
      481 tatttcgtta aggtaagccg ttaaggaatc ttatgaattt cgggccgact gtatgggcct
      541 ctggacttgt cccaaacagt ggtttttgat cgcacggttc ttttgcaaac cgatgtgaac
      601 cccgtaatgg tcaagcttgc aaactgccgc ggaaggtatc cgcttgtttt tagggtcgag
      661 cgactgaggg cgcactatag atcgaaagcc gagatattac aagagtgtat tggaagattc
      721 tctaaaactt ttttggtgtg ttttttgtat tgcaagtttt ttaaagaagc aatagactgc
      781 accaccattt ctaccgcaag cccagatcaa gcaactttct aggaggaaac aaatgagtag
      841 ttcgcgccga gaattcttga agcgaggtac gttcgtggca cttgcagcgg gagctccgct
      901 ggccctcacc gagaaagcct ttggcatcgg cactaccaaa tcaacggcga ttggtttgaa
      961 tatggctgca ttcaaatctc agctgggcac taattttcta attaatcatc aagcgtcgaa
     1021 agtgaagttc acgctggttg acgtaactga ctttgcatca cggaaacaga gtgcggcagg
     1081 aaaagaaggc ttttcactac tcttccgtgg tccaaaagag tcgactctta aacaggatac
     1141 gtatctgatc gagcatgaac agttgggtat gttctccttc ctgatcgttc cggttggaac
     1201 ccaagacacg cgcgctccat actacgaggc agttatcaac cgcctgcatt cttaacttcc
     1261 gttttacaac cagggctcac ggcgtgaggt gttgactggg gttgcactgc aacagcagat
     1321 gaattccgtg ttgctcgctg ggtgagaaac ccaaacgttc aaaaagacta cgtgaaggat
     1381 taaattcctc cacaaaaatc cgtgtgattt ttccggagcg gtttgcttcg tcgagcagtt
     1441 gtttgatcag atagctgcca atcccggcat tccgctctgc gggcagcaga gtgatatcaa
     1501 caatgcgaat ctcatggtcc aaccgcgcca cgtagagacg gcctacgggt cgatcgtcag
     1561 acaggattat gtcgtggttg gccgcgggat atgtctgggt gtagtgatct gtttgcgcgg
     1621 tgaactggtg ttggataaag gcgtcgcgct gttcgtcggt ccagggcact aaggccatct
     1681 cttccgcccg cgtgctgccg taaacttcaa tgagaaaatc tcgatcgtcc ggaccaaccg
     1741 ggcgcagtgt aacgtttgta ctcattggcc tgactgctcg cgaatacgat tgaatactgc
     1801 ttcgtagtaa aagccctcgg cgtcctggct gacgggcact ataaacaact caaactcgcc
     1861 catttgatcg tgcttgagga accgcacgcc ctgatcgaga aatgcttcgc gtggaccacg
     1921 aaacaaaatc gtaaattcct cctgctgagg atagatcttt agttccgaaa cctcgacgag
     1981 atccaattcc accggcgggg gctggtccag ctgaacctga aatttcgtgt ttgcgtgttt
     2041 cgaaaattcc tcgtgcgtta aagaagcctc cattcaaatc tcctcttcag gtttgtgcat
     2101 aatcaaaacc agaaagtctc tgatgttagg accgaaatga aatcccttgt gcagcggcga
     2161 gaaagaatac ccgatcagtc ctgtctgttc taccacactc cggcatcgtt gttcgaaatc
     2221 tgatttggaa atcaccgcgt aaggaagaac ctctccgtaa tccttcaaca cgtgattcag
     2281 gcagagcaga tgctcttcgc gtccatcaag taccaaaacg tccgtatcaa aattcagaaa
     2341 ctccttcaag gtcagcaaac gacactgaaa gcctactttc gcggcacagg cgaccacgtg
     2401 gccaaagtga atcgtaaact cgtcgtggtt taagttgaat gacgcagccg ggtaatgctg
     2461 ctccgatcca tactccgaga ggatcagtgt cccacccggc gttaaatgtt tccacgcacg
     2521 ttcgaggaaa cgaaaaactc cagcattaac gacgaaagaa tccggagcat ctttgtccgt
     2581 gagattgtat ttgtcgagat agtaagcgcc ttctccctgc cacgtcccat cattactacg
     2641 ctgcagcgac gcgactggaa aatccgcgat gacttcattc gagatgatca ggtcaaaggt
     2701 gtggtccgga agatcaaact cggtcgcgtc ctgttggaaa tgtctcgact caggtaagag
     2761 ctcggccagg atctttcttt gactcgccat caaagccggt gagagatcca ggatgtgata
     2821 gttgatactg gtcccattca cacttgcggc ttgttgtaag aacgctcgcg caaaagttcc
     2881 catgccgccg ccgacttcca gcacttccag cgcgtttgaa ttattcagca aaggaactac
     2941 ttcagtcctc agcgttgcca gacaaaagcg cgagccataa ttgagtccgc cgagcgcttc
     3001 gtgcggaaag cggaagccgt ggtttacggt tggttcgatt tgatcgaatt cccaggctgc
     3061 atcctcaatt ccgcttaaat gaaaatcggt aattccatcg ccgtcttttc gctccgagtg
     3121 ataaaggttc cgcggaatta tattgaccag ggaaaaaggc tcgttgagct cctcaggccg
     3181 cacggtgaat ttgatgagct gccgttcctg gcttgttaag aaatcgatcg ccgatctgaa
     3241 ttccgaatcc tcgacctgta actcgttata aacctgccgg agcgttttgg tgccgtccgc
     3301 cttcaacaga atgtcagcag ctaccggcga caatttttct tcgacaattt caggcagctc
     3361 gccgagtcga cgcgagtaga cggcttgcag cattgaagtt cggaccagga tccattctcc
     3421 ggttttcgag cggtatgcga ttgcgggatt ttgaatcggg cgagtcacgt actgatcgag
     3481 caggagctcc agcggatcgt gccccttcgg gatcaggaac tccatttgga ccagttgtcg
     3541 catttgaaga tcagcttcat tgatcaaaag tggggtgccc gatttggcgg ccagcaacag
     3601 actgcggacc ctttcgtccg gctcagctat cacacctgtt aggcggtgaa agaccgcgtg
     3661 ttggaccaca cccggaaaca cacgactctc gacgaagctg atgtagggat tgatcccgta
     3721 ggtaggcatt aattcaactt gtagcacgac ccagtgtaga aaaagcaaat gggaacgcag
     3781 ctttcggtcc tggcctgcga tagcaggcgt aagcataaag cctgggtcgc cagcccaggg
     3841 atcccgtcgg caaaatttgg cagagcccgt gaacacgggc gacagctttt aagctcaggc
     3901 gctgtcgctc gcttcgcgag ctccaatgat ttttttcttc cgatcctggg gcttgcgccc
     3961 caggctttac gcttacacct gctatcgcag gctaagaaac atcagcagga acccaccgca
     4021 tttccaaata tgcgttgtcg ccgccggtgc gacggaatcc cagacgttcg taaaaccgaa
     4081 ccgccggaga tgaggccagg acgtgcagtt ggatcggttt ccctgcggcg gtagcttcgt
     4141 tcattagatc ctgtactaaa cggctcccta agcgcgcgtt tctaaactct gtcaaaaggg
     4201 cgatgtctcg cagtagtata gtcgtgtcac ttctatcaac caggattctg ccaaccggtc
     4261 gtccttcaaa cagaatgatt cgattatcga ctcgcggata aacccgttcg cgagcgagaa
     4321 actgcatctt gatgaacgct tgcttctgct cgtcgctcca accgaatccc tcgagctctt
     4381 ccagacgcgt gctggcataa acctctaaca aaaagggctc gtcttccgcc gtcacctcac
     4441 gcagggaaag attcgtttcg tcgttttgca ccataaactt tcatgccgcg gattaacgcg
     4501 aatgaacgca gatctgcgtt tatctgcgtc catccgcggc taaaaatctt ttcaactgct
     4561 ctctcaaatt caggttaccc accaggcgtt gcaagtaatt cggatgcact cccagaagct
     4621 tcgccgcatc gctgaaatta cctgacgttt gctccattgc gttcaagata atttgcttct
     4681 tcgcttgtgc tactgctcca tgatacgaat ttagattaac cacattaacg tctcgctcga
     4741 gcaacacctc cggcagatct tccgccagta tcagatcggt cgtcccgagt acgacggcgc
     4801 gctcgatggc attctcgagc tcgcgcacat tgcccggcca atcgtgcgat gtcaaacgag
     4861 cgcgcgcttc ttccgaaaag cctttcaact tgcgattgga acgcgtgcca aacttgtttg
     4921 cgaagtaatt cgcgagcagc atgatgtctt cgcgacgctc acgcagtgcg ggcatctcca
     4981 gcgagactac attgagtcga taataaagat cctgtcgaaa ctggcccatc tcgatcgctt
     5041 cttcgaggtt cttgttcgtt gccgcgatca cacgcagatc cacattgatc gttactgttc
     5101 cgccaacgcg ctcaaactct ctctcctgca gcacgcgcaa cagtttgact tgcagcggcg
     5161 gcgttaactc tccaatctcg tcgagaaaaa ccgagccgcc atcggcgatt tcgagccggc
     5221 ctttcttctg cgcgaacgcg cccgtaaacg agcctttctc gtgcccgaac aactcgctct
     5281 ccaataacga ctcgttgagc gccgcgcagt tcaccgccat gaaaggcttc tgagcgcgct
     5341 tgctgtttcg atgaatcgca cgtgcagcca gctctttgcc cgtgccactc tctccactga
     5401 tcaggaccgt cgcctcagtc ggcgccactt tcgagatgaa gtgataaacg cgctgcatcg
     5461 cggcgctttc gccgaccatg ttgtgctcga tattcacgtc cgtccgcaga cgctcgttct
     5521 ccgtctcgag ccattcaaac tgtcgcgcgt tctcgatcgc gaccgcagca ataccggcaa
     5581 tcgcagtgac gagctgcagg tgttcgcgat tgaaatgatc gcgtgtcacg gtgctgtcga
     5641 gatagatgac accgagtttg cgatcgaaca gaatcagcgg cacacacatc accgagtgcg
     5701 tacgcgcttc aatcaaactg tccgtgacga gtccgctttc cgagggcgcg tcgtttgcca
     5761 acagcgcaac accgtcacgc aacacctgct gcacgatcgt gcggctgaca ttgactgccg
     5821 cgtctttgcc gtagactcga tcgagaccga agaccagact cgtttcgaaa gagctctcgt
     5881 cagtaagcag gatcgcgccg cgctctgcgg gcacgacttc gaagagcaga tcgagtagtc
     5941 gttcgagcag attatcgaga ccgcgaatcg agttgatctc cgtgctgatc ttcatcagcg
     6001 cgctgagatc gcgcgtcatc tggtagatcg cgtcgtcaaa tcgaatctgg actgtggagc
     6061 cgctgacgac gtgcgcttca tcgatcgtga cgtcacttga tttcgaagtc gcgtcacctt
     6121 cgtgcgtcag aaacagaaac tgcgaatcgc cgatgcggac gcgatcgccg tgctcgagca
     6181 gtcgactcct gatcggcaaa tcgttaacaa acgtgccatt gagactctcg agatccgtga
     6241 ttacgaatcc tgtttcgtgc ttttcgatct gcgtgtgacg ccgcgagaca gacgcgtcgg
     6301 cgatgcagag attggcgctg gtctcacgac cgatgatgag cgactcttca ttaagggcga
     6361 agatagctcc ttttagtttg ccggagatgg ctgcgagacg aggcggcatg gcgggacatt
     6421 ctaacagcat cagagccatc cacagattac gcagaaaaat ttatctgtga aatctgtgga
     6481 tgttttttgt tacggtaaaa tccgccaatt attattgggc ttcgttggca ccgttttatt
     6541 cttctcaacc caatcaataa tcagctcgcg tatctcttca ctcgaccggt agaccaccgg
     6601 cgcgttcttg tacatgtcgt agccaccacc gccgttgacg cgatagttgt tggtcgcgag
     6661 tcgaagcttt ttcgtaagtg acaagggctg accgtgaaag cgcaggtttt ggatccgctg
     6721 accgatcggt ttcgagatgt cgagatcgta agtgacgcct tcagcaatgt cgaagttata
     6781 agcgggaatt ttctcgtcga cgagatcgct cgccgacttg cccggaacgt actccttgaa
     6841 atatttcgcg gagtgttcca gcgcgtcttt gagctgttga cctgttactt ccaacaccac
     6901 gagcgtgttt tcataaacat acaagcccgc gatatcgcgc acactcaccg gacctttcgc
     6961 gatgcgcgcg gccgggttga aactcgccac catcgacacg tccgctttgc ccgcgtccaa
     7021 ctgcactttc tgaatcagat cgagaatcgc cgtatcgcga aagcgcgcgt cgcgtgcggt
     7081 taactcctcc ggcgactcac cgatctcgcg cgacaaccac gcttgcgtct cacgatcgta
     7141 aggctccgca atcttcacca cgtcaggatc cggctcgaca cgatcgtcag tcggcatcgt
     7201 acgcgccgac ttcgcataca tacgccagcc cgtaccggct ttctgcaaat acagatcggc
     7261 gcgcgcgaga tgtcggcccc aatagttcgc ctgcgtcagc aacacgccgt tgatgtaaag
     7321 cgaaggcacc tcgcgatgcg tgtggcccat gaagatcacg tcaacacccg gaacttcctt
     7381 cgcgatcagg atcgcttcgt tttcgtgaag cacctggccc ggactcatca cacccgtgct
     7441 cagatcttct tcgagcccca tgtgcatcgc gatcacgacg acgtcagctt tctcctgatc
     7501 gcgcagaatc ggaacccact tgcgcgcttc tacgagtggc tcgtgaaatt cgagaccggc
     7561 gtagtttggt ggattgtccc agttgggaat gccgggcgtc gtcaaaccaa ggatgccgat
     7621 tttcacaccc gcaacttcct tgatgatgta aggcttgtaa tgtgtttgct gtttggccgt
     7681 gtcgtacgtg ttcgcggaga gccacgggaa ttttgcttcg ccccgtgctt tctcgaggac
     7741 cttcagacca aagttgtact cgtgattacc gactgtcatc gagtcgtagt tcagtgagtt
     7801 catcacgagc atcatcggat cgggcggcgc gttgtttttg cgaccgtgaa acgactcgag
     7861 cggcgagccc tggatcgtgt cgcccgaatc gatgagcacg acatttggct gttctttgcg
     7921 aatgcgcttg atcaacgtcg cgactttcgc gatgccgcgg ttgtcgggtt tgttggtgta
     7981 gtaatcgatc gggttgatgt tcccgtgcag atccgtcgtg ccgaggatcg taatttgtac
     8041 gcgctccggg gcgaagctgc ggcccacgag cacgagggca agcagcatga ttaagactga
     8101 tagggagtaa attaatggtg acttgtgcgt cttcatattc ttatccacag attacacagt
     8161 ttacacagat tacagcctat tacattagct ttctgtgtaa tctgtgtaat ctgtggattc
     8221 aggttatact ccctgcaaat ggataacctc gtcacttcaa acattcgcca acgaccgatt
     8281 cgcacgctcg taagtgtcgc cggcgtcgcg ctcggtgtgg ccctggtcat gctattcacg
     8341 ggcctcgcgc gcggcatgtc aaatgaactg cagcgtcggg ccacgaacgt ccgcgccgaa
     8401 ctgatcttca tgcggcccgg cgcggtgcaa ctgaccgcca cgaccgccaa tctcgatacg
     8461 agatacgtcg atcggttgaa gcagatcgag ggcatcgaag aggcgctgcc ggtaattcgc
     8521 catttcagcc agggcacgaa aggaatcggt atcgagcaga ttgacggcgt tgactgggac
     8581 gcttacgcgc gcatgaatgg catcaacatg gtcgcgggaa atgggccaca gagcctcgac
     8641 gaagtggtga tcgatgaggt taaagctgag caaaacaatc tgcacgtcgg cgatcgaatg
     8701 aaaccgctgg gcacacagga atatcgcatc acgggaattt attctcccga atcgggagcg
     8761 cgcgtgaaga tgtcgctggc ggcgatgcag aaggcacttg aagcggaagg taaatgtacg
     8821 ttcatctttc tgaagcttcg taattcggac cagctaaagg aagtcgcgca gcgaatcgac
     8881 cgggagttgc cggggaatac gatccagccg acgcgcgaag tgtttacgag ttttgagaag
     8941 agcatcccgc tgcttggcgt gtttttgcgg gtgctggttg tgctcgcggc ggtggtgagc
     9001 tcgctcgtgg tgatgctggc gatgtacacg acgatcacag aacgcacgcg cgagatcggg
     9061 atcttgaagg cgatgggcgc gtcgcgcggt tacatcgtgg ggatcatcga gaaggaagcg
     9121 atactgatca gtctgatcgg gttggtagca ggatttgtgg tttcactcgt cgcgggatat
     9181 ctcattcaca aagcttacgg gttggtgttt gagtatagct ggcaatgggc ggttgtggcg
     9241 ggaacgattg ggattgttgg tgggattttg ggcgcgctgt atccggcatg gcgggcttcg
     9301 aatctggatc cagtcaacgc gctggcctat gaataagtcc tataggacga ataggaccta
     9361 ttattcttcc aaattaataa aagcccgatc gcgctccaga agatgtcgcg gccttttctc
     9421 acgatggcga gcgtctcgcc gattcctctc gcgaagccga ggacttttga gacttgcccc
     9481 gttccgcctt catccacgcc ggcccgcagc ggaataaact taaacgccac attgatgatt
     9541 cgattcactg actcgagtat gaacgcctgc gtgagcgttg gcgccaccgg gctgatgaac
     9601 gagagcgtga tgaatacctc cagcacacca gcgaggtgaa aactcatgtc gagtagaaag
     9661 atcgacagaa aactgcgccg gctcgtcgca taaaacccgt aaacgcgatc ttccagcgac
     9721 tgcgcgcgcg gtataagctt gccaacgacc ttcgtcagca caccacgtct acttagaaac
     9781 ccgagcgcgc cactaagaaa tctcgcctgc caatgtatca ggagataaac caacggaatg
     9841 atgacaaggg tcgtgacgag tgcaccgatg ctcgcgtatc gcagcccttt tggcagtgag
     9901 aacgtgagca acagggtcgc agtgcccgag aagataaaga tcgcgactgt cagcgcgtaa
     9961 aaaatgtttt cgatcgcgag cgccgacagc cccaccatca aaggcacccg atctttaaca
    10021 aaaaccgcct tcgacggctc gctcaccgct acgcttacga acggaatgat gttgccgagc
    10081 gcgtcgccca tcaagcgcgc cgcgaatgcg tcgcgaaatg gaagtcgatg tggcggctcg
    10141 atgcagcgcg tccaggcgag cgcgcgcacg acgtagcgaa tactggaaac agcaagaatg
    10201 aggacaaaac ccgcgccgag tcgctgaatg ttcgaaagga tctcgccgac gcccgcgcgg
    10261 ctcacgaaat aggcgaacag aagcagtccc aacagaccga agatgatccc aagtggagca
    10321 aatctcctgc tcccactctg gttctgagtc aaaacatcgt tcgccatcga cggatctcta
    10381 gcaaccaata taccttcatc cacaggttac acagattcat ttaatcaacc tgcgtaatct
    10441 gtggatggct tttgtgcctt tttgcggctt tagtttatga tgcaatgttc catttcaaca
    10501 cccgtaagac gagcatcaac atgagtcaat cccagtcagc aattgcaaca cctgcgccga
    10561 gtccactcac cacacttaca gaagacgaga cgatgtttcg ctccagcgtc cgcgaatttg
    10621 ccgagggcga attgcggccg cgcgtcgagc acatggacga ggtcgcgaag ctcgatccag
    10681 ccatcatcaa acagtgtttc gacctcggct tgatgggcat cgagacgccc gaggaatttg
    10741 gcggcgcggg cgcaaatttt ttcactgcaa ttctcgcggt tgaagagttg tcgcgcgttg
    10801 acgcatcggt cggcgttttt gtggacgtgc agaacacgct cgtcaataac gctttcattc
    10861 gctggggcac gccggagcag aagaagaaat acctgacgcg actcgcgagc gagagtgtcg
    10921 gcgcgtacgc gctgagtgag ccaggttccg gttcggatgc cttcgcgatg cagacgcgcg
    10981 cggtcgagca agacgatcat tacgttttga acggtcgcaa gctctggatc acgaacggca
    11041 tcgaagctga catcttcgtg ttgtttgcga atgcgaaccc ggaagccgga tatcgtggca
    11101 ttacggcgtt cgtggtcgag aaaagttttc cgggtttcgg catcggcaag aaggaaaaca
    11161 agctcggcat ccgcgcttcg tcgaccgtcg agctgattct tgaagattgt aaagtgccga
    11221 aggagaacgt gctcggcgaa ccgggtaagg gctacaagat ctcgatcgag acgttgaacg
    11281 agggccgcat cggtatcggc gcgcagatga tcggcatcgc gaccggcgca ctcgaggccg
    11341 cgctgggtta cacggcggag cggcaacagt ttgggaagtc gatcaaccag tttcagggcg
    11401 ttcagtttca acttgctgaa atggcaactg agctcgaggc cgcgcgtttg atggtctaca
    11461 acgccgcgcg catgaaggat gcgggcgtca acttcgtcaa agaggcggcg atggcgaagc
    11521 tctactcgtc tcgtgtggcc gagcgcgtga gctcgaaagc gatcgagctc tacggcggtt
    11581 atggttatgt aaaggattat ccggtagaga aatactggcg cgattcgaag attggtgcga
    11641 tttatgaagg cacgtcgaac atgcagttgc agacgatcgc taaactcatc atcggaaaat
    11701 gagccgcaaa aaggcacaaa aggcacataa aagagcagaa ccttttgacg ccttatttga
    11761 aatttcattt tgtgcttttt gtgccttttt gtggctaaaa atgatcttat gaatactgat
    11821 actaaagttt tgaagctggt ccggaagagt gagtcgacgc acacgacggc gaccgagctt
    11881 cccgatcata agctcctgat cgagatgtat cgcacgatgt atctttcgcg gcgtctcgat
    11941 gataaagaga ttcaactcaa gggacagaac cggatctttt tccagatcag tggggcggga
    12001 cacgaggcgg tgctggttgc cgcgggcgtg gtgttgaagc ctggctacga ctggttttat
    12061 ccgtactatc gcgatcgcgc cctctgtctg cagctcggca tgacgccact cgaaatgttc
    12121 ctctctgccg tgggcgccga agccgatccc aactcacacg gccggcagat gccttcacac
    12181 tggggccaca agaagctcaa catcgtctcg cagtcgtctc cgaccggaac gcagttgctg
    12241 caagcagtcg gttgcgctga ggcgagttat cgcttcgagc tcatccccga actgcaaaag
    12301 cgtgtcagtg gttttgaaga cgacgaagtg acgtacgtct cggtcggcga cggcacgacc
    12361 agcgaaggtg agttctggga agcactcaac accgcgagca atctgaaact gccggtgctg
    12421 ttcctgatcg aagacaacgg ttacgcgatc agcgttccgg ttgaagtgca aaccgcgggc
    12481 ggcgacgttt cgcgcctcgt cgagaatttt ccgtcgcttt acctgcaaag atgtgacggc
    12541 accgatccga tcgagtctct cgaaacgatg cggcgcgcag ttgaatattg ccgccagcga
    12601 aaaggccccg ccctcgttca cgcgaaagtt attcgtccgt attcacactc gctttcagac
    12661 gatgaaaagc tctaccgccc tgacgaggaa cgcgccagcg acgccgagcg cgatccagtc
    12721 aagcgttttg gcgcgctgct gatcgacgaa ggcgtgatcg atcaggacgg cctgcaacag
    12781 atcaaagatg aagtcgacgc gctggtgaat gaagcggccg acatggcgct cacgagtccg
    12841 caaccagcgc gtgaaaccgc gacgaagtac atcttttcac ctgacgtcga tccggctagc
    12901 aaagagtttg atacggaaga cagcgtccaa ctttccggca atcccggcac gatggttgac
    12961 ctgatcaatc gttgtctcga tacagagatg gcgcgcgatc cgcgcatcgt cgtgttcggt
    13021 gaagacgtgg ccgattgctc gcgcgatcag tatctcgaag cggtgaaggg caaaggcggt
    13081 gttttcaaag tcaccgcaaa cctgcaacgc aagcatggaa gcgcgcgcgt tttcaactcg
    13141 ccactcgcgg aggcaaacat cgtgggacgt gcgatcggca tggccacgcg cgggttgaag
    13201 cccgtggtcg agatccagtt cttcgattac atctggcccg cgatgcacca gattcgcaac
    13261 gagctcgccc tgctgcgctg gcgttcggga aatgaattca aagcgccggt tgtgatgcgc
    13321 gtgcccgtcg gcggttactt aaaaggcggt gctatttacc actcgcaatc cggtgtcagc
    13381 acattcacgc agattcccgg cctgcgcgtg atctatccat caaatgcgct cgacgcgaac
    13441 ggattgctac gcactgcgat tcgctgcgac gatccggtgc tgtttctcga gcacaagcat
    13501 ctctatcgcc aggcgtacaa caagtcggcg tatccgaacg acgagtttat gattcccttt
    13561 ggtaaagcga agaaggttcg cgaaggaacg gatctctcga tcatcactta cggcgcgctg
    13621 gtccaacgtt ccctcgtcgc ggcgaagcag atggagcagg aaggcatcag tgttgagatt
    13681 atcgatctgc gcagtctggt gccgtttgat tggaacgcga ttgcggaaac agtgaagaag
    13741 acaaacaagg tgatcgtcgc gcatgaagat tcactctcgt ggggctatgg ctcggagatc
    13801 gctgcgcgaa tcactgatga gttgtttgag tatctcgacg cgccggtcaa acgagtctgc
    13861 gcgcttgata cgttcgtcgc ctacgcaccg cagctcgagg atgcgattct tccgcaggca
    13921 tccgacgtcg ccaaagccat cgcagaactg aaaagttatt gacaatattt agccacagat
    13981 aacaaggatc aatccgcgga ttttatccgt gtcatccgtg gctaagttcc ctcaccatac
    14041 acgcctgtat tgatctactc cgcacaaatg ccgcaaacac gcgattgatc gcgccatccc
    14101 aaacggcata aatcttgcgg aaatctcatt agcttccccc gcgttcgaca attaatcgcg
    14161 attgattaga cacaagggag gtacaactga tgaggaagca tttttcgatc gtttgggggg
    14221 cgatggttct gactcttgca ctcgccgcta tcgctctcgg acaaacgaca accgtcacga
    14281 aggcggtcca aaatcccgac ggcacataca caatcattga gtatccagta aagaaagaag
    14341 taatgcttaa tctaacccca gtcaacgtgg ctggagccaa gggaatggcc accattcttc
    14401 gtgacgacga tggaacgagg atcaagctga acttaacggg cgttcccgca gacatgaccg
    14461 cgatgacgct ttatgcggtt gatgacacag gcaccgttac tgcgcttggt ccagttgcca
    14521 ttaccaacgg aaccggcacg ctcgccgcga cgactccgct gtcgaagttc atgttgttcg
    14581 catcaccaga gccggcattg acggcttatg atccgaatac gaaagtgttc ttcaggagcg
    14641 cagtgccgga cggcttcacg gttatcccgg tagcccgtac tggcgatgaa aaagtagcgg
    14701 ctgtcggagc gccagtggcg gcgaccgcgc agaccacgac caccacgacg cccggagcag
    14761 ttgtttcgtc tccgagtgga acgactgtgg ttattccgat gacggaaacc aagaccactt
    14821 acacagcgcc attgctcaac atcccggctt acaaaacggg cgatgacacc aaaatcaaag
    14881 tcgacttcac gggtgcgcta acgggcgcgc gcgcgaacgt tttcatcacg ccgcggaaag
    14941 acggaccgac tgaggtcaag atgcgtttcc acgagctgaa ggaagcgccc gcgggccaga
    15001 gctttatttt gtgggcggtt tcgcctgaca acaagttcat caggctcggt caggttgtga
    15061 ataccggtgg ccggaacgaa gctgagatta gatcagaagt tccactgccg gactttggtt
    15121 tgattgtgac gatggaaagc gctgtcggtg aattgccggt cggcccggcc atcggaacga
    15181 ttcacattgt gccgtaactg acggcgttgg cgttgctctt ttagggtcgc tcgcgagggc
    15241 gacccttttt ctgtgtcaaa tccttcacaa aaaacttccg tcgaagtacc aacttggttt
    15301 aatatatcgt tcgctgaagt tgcctgagtc atcaggtctc ccgacgggct cagatacgac
    15361 ccacttcatc ttttcaataa ctgcgagatc ccctgttcgt gaatcttcac gcgccgaagt
    15421 ctccgttttt gtctgggtgc taacgacatg attaagagaa gctattatcc tcgtctgttc
    15481 ccccttgcat tgattctcct cgttgcctcg tttgggcgtg catcggtgca tgcagacgaa
    15541 actctcccca atctcgtaaa gagagtaaag ccggcggtgg tcgccatcgc aacttacgac
    15601 gcgaatggcg aagcgctcat gaccggcagc ggtttctttc tgcgacccgg ccaggtcgtg
    15661 acaaatcttc acgtgattcg tggcgcggtt cgcgccgaga tcaagactct cgacggcaaa
    15721 ggcaaaattt ttccggtaaa cgggacgctg gcggtcgatg aagaaggcga cctcgcgttg
    15781 ttgagtgtgg agatgccggt ggcgaacgcc atcagaagca ctgagctcgc gaaagatctg
    15841 ccggatgaag gcgaaacgat ctttgtgatc ggtaatccgc tcaagctcga aggctcggtg
    15901 agcgacggga tagtttcggc cgtgcgggaa gtgccgaaca gttatcgcat cattcagatt
    15961 acggcgccga tatcacatgg aaacagcggt agtccggttt tcaatctgaa agggcaggtg
    16021 ctcggtgttg tgacgataaa agtcacgaac ggacagaaca tcaatctcgc gatcgcagcg
    16081 gcgcgagtgg cggagttgac ggctgggaaa ctgcaaccgc tgtcggagtt ggcgctcaaa
    16141 ggcagaggcg acgtggctga gtccctttat cgctcgggac tggattcgct ctggctcggt
    16201 aattacgaca acgcggtcgg ttattttgaa aacgcggcga acaagaatcc gaagcgcgcc
    16261 gatacgtggg tgcaggttgg ctactgcaaa gtgaagcagg gcaagaacgg ggaagcgatt
    16321 cgcgcttacc agcaggcgct gcagctcaag cctaacgatc cggagattta caacaagctt
    16381 ggcgatgctt attactactc gggacgtttg cgtgaggcta ttgactcgta ctccgaggcg
    16441 gcgcggctgc gtcctgattg ggccgagact ttttacaatc tggcgatagc gtattccgag
    16501 agcggcaatc caagcatggc cgctcagcaa gcccggatcc tccagcgact ggatccgaaa
    16561 ctctacgaga aataccttcg ggaaacgatg taaacgcgga tgacgcggat ttaaatccgg
    16621 atttcatccg cgttaatccg cgttaccctg cctgaccggt gaagacgcca gtgtccacac
    16681 tcttcgggtt acccaacacc acgaaccgaa tattcttcat atacttctgc gagacgcgct
    16741 gaatatccgc cggtgtaacc gcggtcagct tctctaggaa gttcactgaa tttctccagc
    16801 cacctccgat caactcatac tgcgccaact caccggcctg cgcggcattc gtctcctggc
    16861 cgaggtaata cgtggtcaga tattgggcca caaccgcctg aatatcgtca gcgtccaccg
    16921 gttcagtctg caagcgcgcg atctcgctca gcatcacacg tattgattgg ttcgcgtcta
    16981 ctgcagtcac atacaaacca ccgacattcg ccgcctgcgt tcgcagaaat gcgtccggcg
    17041 catacgacaa atttcgcttc acccgaactt cttcaaacac acgatcgcgc aacaacgagc
    17101 tcgcgatacg catcgcgtaa atatcgggcg acgtcagcga cggcgccgtg aacaacccct
    17161 ggatgtagtt tgtcggcaac tcgcgcggcg tgacttccac cgtggattta tcaaacgcaa
    17221 gctgcgggat cggtttcgcc atgtagttac ccctcggcaa cttaccgaag ctcgccgcca
    17281 ccagcccccg cacgtcgttc gggttcagat cgccgacaat cacgagcaac agccgcgacg
    17341 tctccatcag cttcgtgtga tatgcgcgca gatcgtccgg cgtgagcttg cccactgttt
    17401 ccaccgtgcc gttcgtgttg ttcacatacg gatgaccggc gtaggccacg cgctcctgca
    17461 gtttttgcag gtacacgtca ggattgtcag tatcatcact gagcgagacg acctgtctct
    17521 gctgcacgag ggccacatct tctttcgtaa aagacggccg cagcgccacg tcggcaaaca
    17581 tctcccacga gcgatcgaag tgcatgcgcg tgctggccag cgacagcacc gagtaatcat
    17641 tgtttgagct gctgccgatc acggttccca tgcgcgacag ctcgctgcgc attttctcac
    17701 gcggaaagtt tgcgctcgct tccgtggcgg cgctgagcat caacgtctcg atgcccgcgt
    17761 tttccgcgtt gatattcgcc gcaccgccgc gaataaataa tcctgccgcg acggtcaaac
    17821 ttccttcacg tcgcttcagc aacactttca gtccgttaac ttcaaattcg ctgaccagcg
    17881 ccgcctgctt ggcaacgacg ctgtgaacag tctcctgcga ttgcggtttc gtttgttgag
    17941 gctgagtcgc ttgcgaataa tccgtgctgg cgacaaacat catcgcgacc agcagcgcac
    18001 tgctgatcag ccgccgcgtc aacttagcgc gaaaagacat acccgactct tgttctgact
    18061 ggttgataaa ttggctcatg attatcctga cgttctactg acctattaac tcttccggcg
    18121 taagttttac tttcgcttgc gcgtcgctgg acatgagggc gaggccgacg tgcggcttac
    18181 cctggatgta cgtggtgatg tagcggctga tgtcggcccg cgacgtggcg cgcaaccgtg
    18241 gcaggtaacc gcggaaatat tcgagccccg tcgacgccca ccagaaactc agcgtgtgtg
    18301 tgtagtcgct cagcttctca cgcgaataaa gatcgtcggc ttcgagtagt gccttggcgc
    18361 tttcgagctg ctcgtcggtg tagtagtcct tgtcgttgaa gtgggccact tcttcgtaca
    18421 tcgctttgat cgccgcgcgc gctttgtcgg gagtggtttg cgcgatgagg gcgattggac
    18481 caacgttgcg ctgcgtgtag tagctcaaat cgacgccggt caccaaacca gtatcgacga
    18541 ggtttctctg aaagcgtgaa ttcggctgtc gcaggatgaa tgagaaaacg tcggctgcgt
    18601 aagtcgcggg attgtcttta ccgatcgacg gtccatgcca gccgatgttg atgatcacgt
    18661 tctgcaccgg ctgctgaatc actgcgcctt cactctttgt gagcggcggg tgatccacga
    18721 gtggaaactc gacaaacgga tctttctcgc gcttgggcca atcgccgtaa agctcctgcg
    18781 ccatggcgaa tgcttccgtg ggattcacgt cgccggtcaa cacgagtgct gagttgttgg
    18841 gaacgtagta acggccctga atgagccgca tcatatcggt ggtggcgccg cccactgtct
    18901 cgcggtttcc gagtgcgttt ttgcggctgg gatacttgta aaagagacga tcgttcatct
    18961 ctctcgccag gtagaagaac gggttcgact cgttgcgatc gatctcgccg atcacgacct
    19021 gcttctcacg accaaattcg cgctcgtcaa agagcggata acgcaccgag tctttcatga
    19081 actgcatggc cgttcgaaga ttcgggctcg tggtggtgaa gtagtagtca accacttctt
    19141 cccgcgtggt gccgttgtaa gcgataccct ttgacccgat ggcgcgcacg taatcttcag
    19201 catttgcgat cgcgcggttc tgcttgaaga acatgtgctc gtaaaggtgc gacagaccgt
    19261 tcagctcggg cggttcggtg taagagccgt ttctcaccgc gagttcgatc gtgacgagcg
    19321 ggatcgagtg atcttcgagc acgatgatct cgaggccgtt cgcaagcacc ttgctgatca
    19381 ggtttgcttc cggaagtcgc gccggagtgg ctgcagtcgc cggcgccgtg gtggtggttg
    19441 gcttcgtcga acgctgttgt gccctggctg gcgtcaacaa cgttagtaga agcagaaaga
    19501 cgagcgaact tcgtttcact gataactcca tatgctggaa attgatgttg atagtggggg
    19561 aaaggttatc ttgaatcttg taaatcctgc aaatcttgtc atcctgtcac agttttctga
    19621 aggattacac gatttacagg attcacaaga ttacttcctg atcatcgatc gtgctttttg
    19681 gcggagggcg gtgcgatagg cagcggcgtc agatccagcc gcgttcgata accattgaat
    19741 gcgcggagtg ccgcgcgggg tggtttcgcc tgtcatctgc atcgagtaag tgcgtttgtc
    19801 attcggtacg aacaggacga gccggcggag gctgcggctt tcgcttgaat cctttcctga
    19861 aaatattaat tcttcaaaac catcttcatc agcgtcgact agatcagagc tccagctcgc
    19921 atgactgaat ccttcggcat cgagccgtcc ctgggcgctg agccgaaact ttccattctg
    19981 tttctgaagg agcaggaaat ttgtcgtgcc ggctttccgc tgattgttta tggcaactat
    20041 cgcctgtccg gagtcggtat cgacgatctc cacatcaccg accgagcctt tcgcaaaacg
    20101 cgcggcgatt ttgcgtgcct gatcgagtgt taactgttcg acgggttttg gattttcgcg
    20161 cggtgtggtt acgccaccag acggagccgt cgagctatcg cgccatgaag ctggagtaaa
    20221 gtgcgattgt gacgcagcca caggaagagc gctgtctggt atgcgtggct cggtcgcact
    20281 ctggccgccc gtgtaacccc aaacgagtaa tgcactgaaa atcgttacga ggatcgtcgg
    20341 cgcgccgact cgaaacagca aaccgcgtcc ggcatcagca gcggcaacca tcgacgacat
    20401 cggatccgca ggcgccaccg gaccctccgg tcgcagcagg ctgcgctcca aaagcatctt
    20461 cgccatcgct gccgtgcgtg gtcgatcctc cggccgcttc gcaagcaaac tatgaactgc
    20521 gagcgcgagt gaacgcggaa tgtcctgtct caatctcggc aacggcggcg gggccgcggt
    20581 cgcctgtttg actaacaacg ctgattgcag tggatcgacg aacggcggac gacccgcgag
    20641 catttcatat aacaacacac caagactgta gagatcgcta cgcgcgccga cgcgttgtcc
    20701 caaacactgt tccggcgaca tgtaacgcgg cgttccgatc aactggcccg aaccagtgct
    20761 cgcgtgtgtc gcagttgatt ccgctgacga agccaaaacc ttcgcgacac caaaatcgag
    20821 cacacaaacg cctcgtttgc cgagcagtat gtttgaaggt ttgaggtcac gatgaatgat
    20881 cccgagccgg tgtgcttctt cgagtacgtc gcagatctgc gttgcgatat cgattgcttg
    20941 tgtgaacgag aagcgaccct ggcgtgcgag cgcctgcgac agagtttccc cctcaacaaa
    21001 ctccatcacg atgtaatgac gcccatcagg cagatttccc gcgtcgaaga tcatcgcggc
    21061 gttcggatgg cgcagcttgg ccatcgttcg cgcttcgcgg agaaagcgat ctgagacgtc
    21121 ggcgtcagac gcaaactcag gcctgataat cttaactgcg acggggcgtt cgagatcgag
    21181 gtgcgtggca agataaacag cgcccatgcc gccggtcccg atttgacgct ctattctgaa
    21241 tctgccagcg ataagttggg ggacatccat actgaccttc cttcgggtta aaaggtcgaa
    21301 atcaacttcg gttttggacg tggctggggg aagaggcccc gtagtttagc ataaatccaa
    21361 ccacagataa cacggataaa aatccggatt ttagatccgt gttatctgtg gctcacttat
    21421 ccaggccggg atgaatgcct gggacatttg ggatccagaa catctgcccg tttatctcaa
    21481 gactgcgcag cccgtttttc ggcaggtcct tttcctttaa cccgagatag gtgaagtgtg
    21541 gcaagtcgct gagcacagtc tgaaaccaac catgctttgc catgatctcg cgcacgcgcg
    21601 ggttgtcaaa ctcgttcaca tcaaacgcca gcatcgcaat gtgttgcgac gcgccgggcg
    21661 ccgcgatcga gtaaaggatc gactttgaca agtccttgct gaagtaaatc ccggttttct
    21721 cgagctccaa aacttcggcg acttgctgcc tgagcggcag gcctcgcata cgtgtcacct
    21781 gctcctcggt gagtcgtccc tgcgagagcc agtaatcgag ggctggatcg aagcgtgagt
    21841 tccagaggcg cacgctgtct tcgtagttgc gccgcgcggc ctctgcgcca tcgcgcggcg
    21901 taatgtcgag accttccttt tgtgcgtctt tgcgtgcttt gttgagactt tttagtgctt
    21961 cgggttgaag ctcaatctcg tcgtacccga tcagctccgc ttcgaaaccg gcgtcgtcct
    22021 gaaattttgt tacctgttct tcggtagtaa aaatacagac cggtggcgga gtgactttct
    22081 tattcgcgac gtagatcgcg ccatattctt cgagaatgcg ccgcgagacg ggatcggtcg
    22141 gcgggcagac cgcctcgatc ttgattttcc gtttcttgag ggccttcgcg agggcttcgt
    22201 aaaaaggagt tgattcgtta gggtcgttcc cgtcgcgggc tgagttataa gtcattggct
    22261 cggttggcat gttatccggt actgctatca ccccgcttgc aatagatcga cagactatga
    22321 ggagaatcag gcttatcagt acacgactta ctcgtctgga actcaggcga ccctccaaag
    22381 cgcagagatg atagtggcgg ccgattgaga cggtcaacag gagccgaact atgctaggct
    22441 ccgtcggaat cgtatttgaa catgaaaagc gcggatttgt tcacggaatt gacagcgatg
    22501 taccgcaagc acgggtggca attgcggagt gtgctgctgc gacccgagac gcgcgtcgag
    22561 cttcagcttc ctgagaatct gccggttaaa gaggcgacgt ttgatgcgct gtggttttcg
    22621 cgtccatcgc acgacgatcg cgaggcgtgg gaattaaggc ttttggcaca gacgcagtat
    22681 gcgttgtttg aaacgtttga agctgatgaa actgaagaag aacgagaaga tttgagactc
    22741 gaaatggagg cgcggctacg agaatattta gccacggata acacggataa aaaatccgat
    22801 taaatccgga tctatatccg tgttatctgt ggctaattta ttgcttgccc attttaaccg
    22861 gaatctcgag cttctcgact ggatcggttt cgttttgacc cgggcgcacg attgataact
    22921 tcactgtgct gtaaggcagg gcgcggcgca cgcgcatcag aaattcctca ctcgtgcgaa
    22981 tcggcacacc atcaaacgcc gttatgatgt cgccttcctt tactcccgcg agatccgccg
    23041 ggcggctgcg taaaattgtt cccaacttga cgccgtacgt gttagttcct ggaacctgca
    23101 ctctctctgc gtcaccgtct tcgtaaccga actgagcgcg tccttccggt cgatgatcaa
    23161 actcgcgcgt caatcgcctg ttgtcgtcca gcgagatcgt cgtcagcttg gccgtcttct
    23221 tctgaccgtc tcggatgtat tcaatatcga ctgttttccc aatcggcgtt ttctccatca
    23281 gctcttggat ctgatcctcg tctttaatcg gttgaccgtc aaaactgaga atcacatcgc
    23341 caccgacgag ccccgcctga tccgcaggtc cgcccggtgt ggtgacggct tcgaacacta
    23401 cgccctggtc cgagttattg aatccatcaa taccgatata agacttattg actggggtgc
    23461 gggacacgat gccgttcccg tgcttcgcca gcggcgcaac caacgcggtg aacgcggcag
    23521 caccaacaaa gaacacgagc agtccgatga agatccagga catcccgctg aggcgacgtt
    23581 tctttttcga aagcgcagca gagtcagcga ctgcggcagg tcctgtgtag ccacccgtag
    23641 ttccagtaag tcgaaacccg caattccggc aaaaacgcag ctccgccggc atcgtcgaat
    23701 ggcagtttga acaaattgtt tcagcaacct gcgacatagc ggatccacct attcccggaa
    23761 tcttggcgaa gaatacacgg acagggtcta tacgccaaat aatcttttcc ggttgcatcc
    23821 gagctacttc gatagacttt gggccacgca aaggcaataa tgcggggtat ccgggaggtt
    23881 cttattgtgg aaatcaccag agcactcgca gctctacaac ccctttatgg caaggacaac
    23941 gtggagatca tcacgactga tggcaggcga gtgcgtggga cggttagaag catgaaaacc
    24001 acccaggccc tcaccacgcc cagcgtaaag atcgagatag caaacgggca gatcgtgaag
    24061 atccccttcc cgaccattgc ggagattaag gatcacaacg tcagcactta attttcggtt
    24121 ttcggtggta ttggcgtata gtacgccgtt ctatgccttt gcccctcagc attgacgatt
    24181 tcgggacacg catcgccagc gtctggtctg agctccgtcc cgccacccgt gggctcatcg
    24241 aacgagcgtt acaagccgcc tcgaatggga ctcaaacacc tcgaaatttt caatacgatg
    24301 cgcgcgccga tctcgagctc agccgctttc tcgccgctct cgacgcggag aagaacggca
    24361 aactgaagtc catcgccgac acgtgcgccg ccgttctgct cgagaaaacc gaatcagccg
    24421 aggtgttcgc gcagctcgtc cgccgcgcgg aaacgcaaag agactatcgc cgcatcgaca
    24481 tccttgcaga cgcactgacg gcgcgctttc cgcctagcga gatctgcgag ctcgcacgct
    24541 ctgaagacac gattgtccgc gagctcgcga acgaagcact cgcccgctgc cccgtttcgg
    24601 tgttgactgc gttgctgaac gatccggttg atgcagaaac agcccgcgcc gctctgcgaa
    24661 gacaggttat cgaatacgga tcagaggaag cccgccagat ctcgaacgcc ctcgatcaag
    24721 cagacgagat gtagtacaca aaaggcacaa aaggacaaaa agcacaaaaa agacctttaa
    24781 tgagttaagg cgccttatcc tcgtatggaa gaatttagcg ccttaatttc taaaaggtct
    24841 ttttgtgcct tttgttcttt tgtgcctttt gtgtactact tgcttatcga atggaggcgc
    24901 tcgacgacct ggtctatgag ccagttagga gttgaagccc ctgccgttac gccgactttc
    24961 ttcacgccct gtaaatcagc cggattaatc tcggccggag tctcgatcag aaaactcttc
    25021 gcgcactgtt cctcgcaaac tgcgagtaac ttaacgctgt ttgatgaatg gcggccgcca
    25081 atcacataga aggcatctac ttgcccggcg agtgcacgcg cggcgtcctg tcgatcgcgc
    25141 gtcgcagaac agatcgtgtt gacgacttgc ggctcggtgt cggctttctc gcgaactgct
    25201 tccgcaacgt ccaaaaatgt ctgcaacttg atcgtcgttt gcgaaacgac caacggtgaa
    25261 cgcaacttcg gaagctttgc tacttcattc gcatctcgca cgacgtaagt attgtttggc
    25321 gcgtaaccaa ccacgccgat catctccgga tgatccggat tgcccgctac cacaacatcg
    25381 cgtccctggc gcgcggccct ttccgcgagg tgctgcacgc gggtcacaaa cgggcaagtc
    25441 gcatcgatca ctttcgccgc tcgttgctcg agctcgtgtt gtacttgtgg cgtaactccg
    25501 tgcgcgcgaa tcactgcaat cgtgtcggcg tcagcttcgt caggcgagtc gatagtcgaa
    25561 acgccgcggg cgccgagtcg ctccatctcc tgcgcgttgt gaatcagtgg tccaagtgag
    25621 cgaatcgggc gcgcgccttc ttcgaccacc tcttcgacca tctcaacagc gcgctcgacg
    25681 ccgaaacaaa aaccgaattc ctctgcgagt aggacttcca tgttattgag ctgcctgcga
    25741 ggtccgctgc cgggccttct cgcgccactc ctctctcagc ttgtcgccgc ccgcgacgcg
    25801 gtcgtcgatc cattccattt cccaccgcag cgcattcgtg agatggccga gcagggcctt
    25861 cttaacgccc gccacggagt gagcaatgtt tttgggcata attgcgtatg aacaatcctc
    25921 gatctggcgc aaaccgccta aaacggcctc tgcaacgtca cgaattgggt cccgaccttc
    25981 tgccatatca aacctccaac gtccgttcat tctagcagtt ataactgcgg atcacacaga
    26041 gggatacgga ttaagtcggc aggccgttcc gggcgattga ttccatgagg ttgcagaagc
    26101 gatcgagctc gccgagggtc gtgtagacgt tcggggtgat gcggatgccc ttgaattcct
    26161 cgtgcacgat cggggtcgta aagacgcggt gcttgttgaa aagataactc ccgatcgcgg
    26221 tcggatcgat tccggcaata ttgacgttcg cgatcgcgca cgactggtcc ggatcgaacg
    26281 acgtgttgaa gctgactttc ggtacgtctt tcagacggtt catccagtaa cgcgacaagt
    26341 aacggaggcg tgcttctttg cgttttccgc ctatgccgtt atgaaacagc aacgcttcac
    26401 cgatcgcgag ccgcggcgcc gcggagtgtg tgccgatctc ttcaaacttg cgaatgtcgt
    26461 ttgcctgttt tgactccgcc gccatcaacg gccagagctt ctcaacacgc tcgcgcttga
    26521 cgtaaagcag ccccgttcct ttcggcgcgt agagccactt gtgcaaactc gtcccgaagt
    26581 agtcgcagcc gagatctttc tgtttgaagt caaactgtgc atacgaatgc gcgccgtcga
    26641 cgatcgtctc gatgcctttc gcgcgcgcca gatcgcagat cgcttttact ggcgtgatct
    26701 ggcccgtgat gttcacctgg tgcgaaacga gaatgagctt tgttcgactc gtgatgcctt
    26761 tttcaaacgc ggcggtgatc tcgttgagat ttttgggcgg gatcggtatc tgcaccatct
    26821 tgagtacgag accttcgcga cgctcacgct gtcgtagtgt ggtcaacatg cgcgggtagt
    26881 cttgtgtggt ggtgagtatc tcgtcacccg gtttgaagtc cattcccatc aaaaggattt
    26941 cgagggactc tgacgcattg cgcgtgatcg cgatctcttc gcggtcgaca ccaaacatct
    27001 ccgccagtcc ggtgcgaata gtttccgact gtggctcgag tagttgccac atcgtgtacg
    27061 cagttgcgtc ttcctgttgc caggtgtaac ggacgagcgc ttcggtgacg atgcgaggac
    27121 tcggcgagac gccgccgttg ttgagattca cgatgccgcg tgtgacggtg aacgagtttt
    27181 gaatgatgga ccagtagtct tcatccgtgg ccacctgctc gggcgtgaga tgggccacac
    27241 tgcgcgttgc cgcttcgact tctttcaaga gcgcgccaat ggttggcgtc gtgagcgcag
    27301 cgagaccgag acctttgccg gccagtgaga ggaagtcgcg acgagatttc attggagagc
    27361 ctcctactat gagtgtgtta gacaggattt acaagatttt tcatgatgaa caagactcat
    27421 tcttgtaaat cctgaaaaat cttgtaaatc ctgtctgcta ctgcgtttca taaccgcgcg
    27481 gcacaattgc tttccgattt tcaccagcca gtgagatcac cttcacctcg actttgcgaa
    27541 agttcttttt cacggtgttc gacgactgat aagtaattcg aaattgtgca tccaggtcgt
    27601 gaacgatctg agcgatcgca tggcccagct cggagtcgtc tctgggaaag aacacacggc
    27661 cgccagtctc ttcggcaagt gtagtcaaga gttttattgc ccgctctcga ttacctgcac
    27721 gagtgatatt ggtgggctta tcctgtagat ccaaaatcaa cccgacgacg aagatttgga
    27781 ctgtttcctt atgcaaaaat gcgagaagtt tctccacatt gtagtaactg ttgcgatcct
    27841 cgccatcggt aaagactacc agcgccttgc gccgatccga gttgcgattg tgccctgcta
    27901 tgtgctccac gcccatatag accgcatcaa tgaccgctga ttgcccgccc tccacatata
    27961 tcgactcgac tgcgctcgtt aagagctttt gatcgctggt aaagtcctgc agcgtctcga
    28021 tcttgtcact gcttatgaac cgctcaagaa agatctcatc tttaggatta cggtttacaa
    28081 ccatcagctt agcgcctttt ataacgggct cgagatagcg gaggaacgag ccggaggcat
    28141 cgatcgcaat gcctagatca accggcctta catccgcctc gacactcaat atggtttgtt
    28201 caaccgtatc atcaagtatg cggagatcgt ctttgctaat cgcgtcgaga gacttgtcct
    28261 ttgagtcggt gacaatcaga ctcagtcgta cggttggacc agcaggtttt gccacaggtg
    28321 gattcggctc ctgtgccaaa agcgtagagc tcagtgccaa agaaccgagc acgagcgaga
    28381 gaatcgctgt tttcataatc gacttaggtc gcctcatgga caatctcgcc attgataact
    28441 gtcatcacgc accgcgtttt taatatttca ggcgcgggaa tcgtcatgat gtcagcggaa
    28501 aggattgtta agtctgccag tttaccaacc tcgatcgtac cgcgcagttt gtcttcaaac
    28561 gccgcatacg caggccagat cgtgaacatc ttgagcgctt gttcgcgagt taccgcttct
    28621 tcgggatgcc agccttcacc ggaaaatccc ttctggtcct tgcgcgcaac cgcggcgtaa
    28681 aactcgatca tcggctcgcc gcgctcgacc ggcgcgtcag atccaccggg aacaaccacg
    28741 cccgacttga tgaaactctc ccacgcataa gcacctgcca gacgaggaat gccgagacgg
    28801 ctcggcgcga agaagagatc gccaatcgcg tgcgaaggct gcattgacgg aatgattccg
    28861 agcttcgcga aacgcggaat gtcagccgga ttgacgatct gcgcatgctc gacacgccaa
    28921 cgcggctcaa caacttttct ctcgctcacc ggcacagcct tgagcgcggt ttcgtactcg
    28981 tcgagtatga aacgattcgc gcgatcgccg atcgcatgtg tctcgacttg aatgcccttc
    29041 ttcaaagcgt cgatgagcat cggtcgcagg tcttcggctt tgactgtcag aaatccggaa
    29101 gtgtcgggcg cgtctgagta tggaccaagc agcgcggcac cgcgcgagcc gagtgcgcca
    29161 tctgaaacga ccttgatcgt tctgactgtt agacgatttc caaactcgcc gatgattgcg
    29221 ccttcgttga gcaggcgatt agcttgcggg cctggtccat aaaccgcttt gtagatgcgg
    29281 agcttgagcg tgcccgcggc atagagtttt ttgaagatat cgacgtctcc gtaatcgccg
    29341 ccggcgtcct ggacctgcgt ccagccaagc tcgacgtcgc gtttcgctcc cagcacgacc
    29401 tcacgctcgt cgtcttctct ggttgtcggc ggcacgcggc gacgaacgag accctgcgcg
    29461 gcatcgagca acatgccgtt cggctcgccg gactgtttat ctttcgagat ctcaccgccg
    29521 aatgggtttg gagtgttttt gtcaacgttc gccagtttta gcgcggcgct gtttgcgact
    29581 gcaccgtggc cgtcggcgcg tccgagaatt acgggattgt ttggtgacac tttgtcgagg
    29641 tcccagcgcg tcggaaacac tggcggctgc cagtgcgtct cgatccagcc tcgacctgtt
    29701 acccattcgc ccggctttgc ctgatcaacg cgcgctttca ctttcgcgag caagtcgtcg
    29761 agactcgtcg tgccttcgag attgagtgtc atctcgcgct gaccgacacc ggaaaggtgc
    29821 tggtgcgcgt cagtgaaacc cggcaacacc gtgctgcctt tcagatcgac gacgcgtgtg
    29881 cttgggccaa cgaacttctg cgcggctgca ttcgtgccga cgaaaacgat tttgtcagcc
    29941 ttgacggcga tcgcttgtgc tctcggtgtc ttgtcgttgg cggtgtagac gttgccgttt
    30001 ttgaagacga tgtcggcagg tgttgattgt tgaatcggaa taaagctcac gaacagcaga
    30061 cttgctgcaa tcaaaaagag tttcattttt ccctcggaat agttaggacg cggatgaacg
    30121 cggattatag cggatcaggc gcgcagaaac ttatcgagct cagatttagt tgggagagcg
    30181 gtcctcgcgc cgagggcgct gcacttcatg gcggcgacgg cgttgccgag tttcaggctc
    30241 gtttcgagat tttcgccggt taggaagccg taaagaaagc cggcgtgaaa ggcgtcgcct
    30301 gcgccggtcg tgtcgcggca accacccgga gctgcgaagc ccggcgactc gataaactta
    30361 ccttcgtgat aaaccaccgc gccgtccgcc ccgatcgtca tgcccacgag ggcacaacca
    30421 taacggct
//
LOCUS       DASSFQ010000268         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2216653, whole genome shotgun sequence.
ACCESSION   DASSFQ010000268 DASSFQ010000000
VERSION     DASSFQ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216653"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFH96_09715"
     CDS             complement(<1..945)
                     /locus_tag="VFH96_09715"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6974290.1"
                     /translation="MLVARRRMDHDVFEREFAIESLKSERLRVTILMGAIISSVALLL
                     LMTLLFFEQFNTTFRGNARKFLFAAIVLFGINICYLMAERIALGRMIKKQMKPFPALK
                     YLSAFVETSIPTAGIIIGSYFLGPLYTLFTPAAFIYPVFISLSALRLNVRLCVFTGAV
                     AGLEYTLLAFYFVRQAAGTPVDPVLTALPQHVFKGFLLLVMGVVTGLVTLQIRKRILN
                     AFSVVQERNRISRTFGEYVSPEVMSKLLDLKPDLRSENKNVCVMFLDIRNFTGFAEKR
                     SPEEVVQYLESLFEFMIEIVNRHHGIINKFLGDGFMAVF"
     gene            complement(954..1361)
                     /locus_tag="VFH96_09720"
     CDS             complement(954..1361)
                     /locus_tag="VFH96_09720"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HET6974291.1"
                     /translation="MSNEPEPNATGTATLIVGPQDLASSIASVTGDSFPPVFATARMV
                     ALMEVAASRVLVPLLGPGELSVGVTVDINHTAPTPLGAEVTATARYAGREGKLFLFEV
                     SCADKGGEVGRGWHKRAIVSSERLQSGAARRAG"
     gene            complement(1432..>2128)
                     /locus_tag="VFH96_09725"
     CDS             complement(1432..>2128)
                     /locus_tag="VFH96_09725"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6974292.1"
                     /translation="LFFNLKPGVALGDATNFITKTAAETVPSGVRGELQGEALTFSRT
                     VTSLTILMALAVFVMYVILAILYESYVHPLTVLSTLPTALVGGLLTLLVFGQEASLYA
                     FVGMFMLMGIVKKNGIMIVDFARHRVEAGEPADKAIHDASMDRFRPILMTTLAAVFGA
                     IPIALGYGADGSSRRPLGLVVVGGLVVSQFITLYITPVIYLYLEQFQERVLDRTSFFR
                     SRRSQKQPAVVES"
BASE COUNT          541 a          637 c          538 g          412 t
ORIGIN      
        1 aaacacagcc ataaaaccat cacccagaaa cttgttgatg atcccgtgat gccggttcac
       61 gatctcgatc atgaactcaa acaacgactc cagatactgg accacttcct caggacttct
      121 cttctccgca aaccccgtga agtttcgaat atcgagaaac atcacacaaa cattcttgtt
      181 ctcactcctg agatcaggct tcaggtcgag cagcttgctc atcacctcag gcgagacata
      241 ctcaccaaac gtgcgcgaga tccggttgcg ctcctggacc accgaaaaag cgttgagaat
      301 gcgtttgcga atctgaagcg tgacgagtcc cgtgacgact cccatcacaa gcaaaagaaa
      361 tcctttgaac acatgctgcg gaagagcagt aaggaccgga tcgacaggcg tgcccgcggc
      421 ttgtcgcaca aagtagaagg ccagcagcgt atattccaat cccgccaccg cccccgtgaa
      481 aacgcacagc cttacattca gacgcagcgc tgataacgag atgaacaccg gatagataaa
      541 cgcggcgggc gtgaacaacg tgtagagtgg tccaagaaaa tacgagccga tgatgattcc
      601 cgcggtggga atgctcgtct cgacaaacgc gctcaggtac ttcagcgccg gaaacggctt
      661 catctgcttt ttgatcatac ggccgagggc gatccgttca gccatcaggt agcagatgtt
      721 tattccgaag agcacgatcg cggcaaacaa aaacttcctg gcgttgccgc gaaaggtagt
      781 gttgaattgc tcgaagaaga gcagcgtcat caacagcagg agtgcgaccg agctgatgat
      841 cgcgcccatc aggatcgtga cgcgcagtcg ctcgctcttc agactctcaa ttgcaaattc
      901 acgctcgaat acgtcatggt ccattcgtcg ccttgcaact aacatggcac acctcaacca
      961 gcgcgtcttg ccgcgccgct ttgtagccgt tcactcgaaa caatcgcccg cttgtgccat
     1021 cctcgaccaa cttcaccgcc cttatcggcg caactaacct cgaataaaaa gagcttgccc
     1081 tcgcgaccgg cgtagcgcgc agtcgcagtg acttccgcgc cgagcggtgt cggggcggtg
     1141 tgatttatat cgacagtgac gccgactgac aactcccctg gaccaagcaa aggcacgagc
     1201 acgcgcgacg cggcaacctc catcaatgcg accatccgcg ccgtggcaaa tacgggtgga
     1261 aacgaatcgc cggtcacgct tgcgatcgag ctcgcgaggt cctgtggtcc aactatgagt
     1321 gtggcagtgc ctgtagcatt cggttcgggt tcatttgaca tgaggacatt taaccacgga
     1381 tgagacagat aaaaaatccg attttatccg tgttatccgt ggctaaattc tttacgactc
     1441 aaccaccgcg ggttgctttt gagatcttcg cgaccggaag aatgaggtgc gatcgagaac
     1501 tctttcctga aactgctcga ggtacagata aatcacgggc gtgatgtaca gcgtgatgaa
     1561 ctgcgaaacc acgagcccgc caaccaccac caacccgagc ggccgccgcg aagaaccatc
     1621 cgctccataa ccgagcgcga tcggtatcgc gccaaacacc gcggccaggg tcgtcatcag
     1681 aatcggccgg aaacgatcca tgctcgcgtc gtgaatcgcc ttgtctgccg gctcgcccgc
     1741 ttcaactcga tgccgcgcga agtcgacgat catgatgccg ttctttttca cgatacccat
     1801 caacatgaac atgccgacaa acgcgtagag cgacgcttcc tggccgaaaa cgagcaacgt
     1861 caacagccca ccaacgagcg ctgtcggcaa agtggaaagc accgtcaacg ggtgaacgta
     1921 actctcgtag aggatcgcga gtatcacgta catcacaaac actgcgagcg ccatcaggat
     1981 cgtcaaactc gtcaccgtgc gactgaacgt gagcgcctca ccttgcagct cgccacgcac
     2041 accgctcggc accgtctcag ccgctgtttt cgtgatgaag ttcgttgcat caccaagcgc
     2101 gacgcccggt ttgagattga agaagagc
//
LOCUS       DASSFQ010000269         5960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_728303, whole genome shotgun sequence.
ACCESSION   DASSFQ010000269 DASSFQ010000000
VERSION     DASSFQ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728303"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1274
                     /locus_tag="VFH96_09730"
     CDS             231..1274
                     /locus_tag="VFH96_09730"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET6974293.1"
                     /translation="MFSKDGNTKDVKTPRICEVVWHNGEFINWNDARVHVMSHVLHYG
                     SSVFEGIRCYSTKQGPAVFRLREHMQRFLNSAKIYRMDHEWTLDQLVTASVDLVKHSG
                     MDQCYIRPILFRSLDEEHPAFGVNPFPNPLACYIGAWDWGKYLGDEAIERGVDVCVST
                     WQRLHANAMPAMAKSGANYMNSQLIKMEALLNGFAEGIALDDRGYVSEGSGENIFMVA
                     GDVVLTPPLSSSILPGITRDSVIQICQELGITIKERTIQRAALYVADELFFSGTAAEI
                     TPIRSVDRITVGNGARGPVTEKIQKMFFEITKGERPAPGDWLTYVNEFKSAPSDNNGF
                     SRAAIETPAGTIN"
     gene            1455..2618
                     /locus_tag="VFH96_09735"
     CDS             1455..2618
                     /locus_tag="VFH96_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET6974294.1"
                     /translation="MAKIDDLLRVAIGHNSSDLHLKVGAFPVMRIGGELHPVADAPRL
                     KPEDTLDMAFSMMSNRQKQRFKEVSEVDIGYGVSGLGRFRANIFQQRGTVSIVLRVIP
                     DHTKTAAQLGLPPVIDKIAEERRGLILVTGATGSGKSTTLAAMIDCINSTRSGHIVTI
                     EDPIEFLHRDKHAFVTQREVDVDTCSFAQALRGALRQDPDVILVGEMRDHETIETALT
                     AAETGHLVLSTLHTLDATETITRIVSSFPSHQQKSVRIQLAGILKAVISMRLVRAAKG
                     AGRVPAIEVLVSTATIRDHIINEEKTYLIREAIAAGTSQYGMQTFDQSLFHLLQSRLI
                     TQEEALHNATNPDEFRMRVSGIYSKDDTLNHITTPTITPPPVANEPDSIFVKH"
     gene            complement(2628..4136)
                     /locus_tag="VFH96_09740"
     CDS             complement(2628..4136)
                     /locus_tag="VFH96_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974295.1"
                     /translation="MLQDLTTPNLRESAHQQITSLLREYAEWLFVSADLIQSLRREEI
                     ELNFVQQKLMLSCWTEAGTGVWRLLAWDWNGQLLELKVSRRSGAEVSLIELIPRTTAK
                     VVTATIRAARELRCEQLAQLAALESGTTVERLSLSRGAKPGQPGRYAQIVLRRRRESI
                     AVTGPVVSSPPSAVDAFLSSALLWFGRKSVDQLWLIVSPELLKPMIYRVALLRAGLRS
                     LIKVFTVDEQRTALTAVACPDKEELWKNKLAHFPPVAATRASELTTAIVAAAPDAIDV
                     VHARHGETLRYFGLPFARVRSLLGHEKVWFGIDRAHRRLLDESTLNDWHNLLSDLREH
                     RSSSALDHRHAFYRSAPEAWLESLLRRDITRLDPGLIIAPLHAQFRTARGAKLGIRPI
                     DLLALRQDGRLVVIELKVSEDREHVLQGADYWRRVEAHRRRGHIAKAKLFGSRTISDQ
                     PPLVYLVAPTLRVHPAFETLARCISSDIEIYRFDINEDWRSGVRVMRRKRVN"
     gene            4214..5392
                     /locus_tag="VFH96_09745"
     CDS             4214..5392
                     /locus_tag="VFH96_09745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET6974296.1"
                     /translation="MKNVFLAGAVRTPIGKFGGTLASWTAADLGVAVAKESLHRAGLS
                     PEQVTDSIWGCARQAGGGPNVARQITFRAGVPETVPAATINQACGSGLKAIILAAQEI
                     MLGRADAVLAGGTESMSRVPYFADGARWGMRMGNMQLVDGMYRDGFSDPLSGLLMGQT
                     AEKLARQHQISRQEQDEFALRSQQRAEAAIASERFNDEVMPLEIAGKKGTTSFSQDEH
                     CRAGATLQDLAKLPPVFYKDGTVTAGNSSGITDGAAALVVLSEDGIKRFGTTPQVRIV
                     DYEITGVAPDIMGIGPVPAVRALLERQHLTLSQIDLIELNEAFAAQVIACDRELGFDA
                     ERLNVNGGSIALGHPIGCTGVRITTTLIYEMQKRQAKLGLATLCVSGGMGLALLLERV
                     "
     gene            5395..>5960
                     /locus_tag="VFH96_09750"
     CDS             5395..>5960
                     /locus_tag="VFH96_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974297.1"
                     /translation="MPLRSALILIALVPLTLSCRSQSDKTANNNSNNDTIVSSTPPFQ
                     TREPDRYRAIRTVTIGSPNGQTLTTKNLIARDGDLRRNESQVSSKQIAYLETPEGRFV
                     LVPAEKIYAEVAPGADLPASESDETLERSPEGLLHAETGNTSYQRLGEEALGERRTDK
                     YRVIVNTASAASVSVSETLIWIDNALKMP"
BASE COUNT         1362 a         1724 c         1623 g         1251 t
ORIGIN      
        1 ccggcgaaaa acgctgcgag caaaagtaac acgatcacaa cgcgcacggt gtgtttgcag
       61 cctgcaagcc gtcttgccaa gcgactcaaa tctacggtat tgtgccctgt ttgataaccc
      121 actcagtaca tttaaagagt accggtttcc cccagtgtgg aagtgtctct acacaatgaa
      181 gatgagggcc tttcccctgc atctcagtat ttcctgaagg agtaaacaac atgttctcaa
      241 aagacggcaa caccaaagac gttaagacac cacgcatctg cgaagttgtg tggcataacg
      301 gcgagtttat taattggaac gatgcgcgcg ttcacgtgat gtcgcacgtt ttgcattatg
      361 gctccagcgt ctttgaggga atccgttgtt actccaccaa gcagggacca gccgtctttc
      421 gcctgcgcga acacatgcag cgatttctaa actccgccaa gatttatcgc atggaccacg
      481 agtggacgct cgatcagttg gtcacagcct cggtcgacct cgtgaaacat agcggcatgg
      541 atcagtgtta catccggccc atcctgtttc gcagtctcga cgaagagcat ccggcctttg
      601 gcgtcaatcc gtttccgaac ccgctcgcgt gttacatcgg cgcctgggac tggggcaagt
      661 atctcggcga cgaagcaatc gaacgcggcg tggacgtttg tgtttccacc tggcagcgtc
      721 tccacgcgaa cgcgatgccc gcgatggcaa agagcggcgc gaactatatg aactcgcagt
      781 tgatcaaaat ggaagcgctc ctgaacggct tcgcggaagg catcgcgctt gacgatcgag
      841 gttatgtttc ggaaggctcg ggcgagaata tcttcatggt ggccggcgat gttgtgttga
      901 ctccaccgct ctcgtcttca atcctgccgg gcatcacccg cgatagcgtg attcagatct
      961 gccaggaact cggtatcacc attaaagagc ggacgattca gcgagccgcg ctttacgtag
     1021 ctgacgagct cttcttctcc ggcacggccg ccgagatcac gccgattcgc tcggttgacc
     1081 ggattactgt cggcaatggc gcgcgtggtc cagtgaccga gaaaattcag aagatgttct
     1141 tcgagatcac caagggtgaa cgtccggccc cgggcgactg gctcacctat gtgaatgaat
     1201 tcaagtcggc gccgtcggac aacaatggct tctcccgggc ggcaatcgag accccggccg
     1261 gcactataaa ctaagtacta tccacagatt acgcagattt tcattcataa tctgcgtaat
     1321 ctgtggatga agttattgac gcccctcacg gcgtcaagct atattcgaaa atgcccgagc
     1381 gttcctccca gcgctgacac tcgccagcaa tcctctcgta atcccacaag gtttgcagta
     1441 gttcaggata tttcatggcc aaaatcgacg atttactccg agttgccatc ggtcacaatt
     1501 catcggatct ccacctcaag gtcggagcgt tccccgtgat gcgtatcggc ggcgaactcc
     1561 acccagtcgc ggatgctcct cgtctgaagc cggaagacac gctcgacatg gcgttctcga
     1621 tgatgtccaa ccggcaaaag caacgattca aagaggtttc ggaagtcgac atcggttatg
     1681 gcgtgtccgg tttgggccgt ttcagggcaa acatctttca gcaacgcggc acggtcagca
     1741 tcgtcctccg cgtcattccg gatcacacga agacggccgc ccagctcggt ctgccgccgg
     1801 tcatcgacaa gattgccgaa gaacgtcgcg gactgatcct ggtgaccggc gccactggtt
     1861 ccggaaaatc tactacgctc gcggcgatga tcgactgcat caactcaacg cgcagcggcc
     1921 acatcgtgac catcgaagat ccgattgaat ttctgcatcg tgacaagcat gcgtttgtga
     1981 cgcagcgcga agtcgacgtc gatacatgtt cattcgcgca agcgcttcgt ggtgcattgc
     2041 gtcaggatcc tgacgtcatt ctcgtcggcg aaatgcgcga tcatgaaacg attgaaacag
     2101 cattgacggc agcggaaacc gggcacctcg tgctttccac gttgcacaca ctcgatgcga
     2161 ccgaaacgat tacgcgtatc gtttcctcgt ttccgtcaca ccagcaaaag tctgtgcgca
     2221 ttcagttggc aggaattctg aaggccgtaa tctcgatgcg cctggtgcgt gccgcaaaag
     2281 gcgcaggacg cgtgccggcc attgaagtgc ttgtctcgac ggccaccatt cgcgatcaca
     2341 tcatcaatga agagaagacg tatttgattc gcgaagcaat tgcggcgggc acatcgcagt
     2401 acggcatgca gacgttcgat cagtcgctct ttcatctatt gcaatcgcga ctcatcacgc
     2461 aggaagaggc gctgcacaac gccaccaacc ctgatgagtt ccgcatgcgc gtctccggca
     2521 tctactccaa agacgacacg ctgaatcaca tcaccacccc caccataacg ccaccacccg
     2581 tcgccaacga acccgacagc atcttcgtca agcattagtt ttagccgcta attaactctt
     2641 ttgcggcgca tgacgcggac gccggagcgc cagtcttcgt tgatgtcgaa gcgatagatt
     2701 tcgatgtcgg aggagatgca gcgtgccaac gtttcaaacg cggggtggac gcgcagagtc
     2761 ggagcgacga ggtagacgag gggtggctgg tcgctgattg ttcgtgaacc gaatagtttt
     2821 gctttcgcga tgtgaccgcg gcgccgatgg gcttcaacgc gccgccagta atcagcaccc
     2881 tgcaacacgt gctcgcgatc ttccgagact ttcaattcaa tcaccactaa acgaccgtcc
     2941 tgtcgcagcg ccaagagatc tatgggcctg atgccaagct tcgcaccacg tgccgttcga
     3001 aattgcgcgt gcagcggcgc gatgatcaaa cccggatcga gtcgagtgat gtctcgtcgc
     3061 agcaaggact caagccacgc ttccggtgcg gaacggtaaa aagcgtgacg atgatcaagg
     3121 gctgacgacg atcgatgctc gcgcaagtcg ctcaacaagt tgtgccagtc gttcaaggtt
     3181 gactcgtcga gcaggcgacg atgtgcgcgg tcgatgccaa accagacttt ttcgtgtccg
     3241 agcaacgaac gcacgcgcgc gaatggtagt ccgaagtagc gcagtgtttc tccgtgtcgt
     3301 gcgtggacca cgtcgatcgc atctggtgcg gcggcgacta tcgcagtcgt gagctcactc
     3361 gctcgtgtcg cggcaacggg tggaaaatgc gcgagcttat ttttccataa ctcttctttg
     3421 tcaggacacg cgacggcggt tagtgcagtc cgttgttcgt caacggtgaa aaccttgatc
     3481 agacttctca aaccagcgcg caggagtgcg acgcggtaaa tcattggctt gagcagctcg
     3541 ggagaaacaa tcagccagag ctgatcaacc gactttcgtc caaaccacaa gagcgccgag
     3601 gaaagaaacg catcgacagc agacggtgga ctggaaacaa ccggacccgt tacagcaatg
     3661 ctctcgcgcc tccttcgtag cacaatttgc gcgtaacgac ccggctgccc cggcttcgct
     3721 ccgcgactca acgacaatcg ttccactgtc gtcccgcttt ccaacgccgc cagttgggcc
     3781 aactgttcac agcgtaactc acgcgcggct cgaatcgttg cggtaacaac cttcgccgtc
     3841 gttcgcggaa tcaattcaat gagcgaaacc tccgctcccg accgtcgcga gactttcaac
     3901 tcgagcagtt gtccattcca atcccaggcc aacaaacgcc acacgccagt gcccgcttca
     3961 gtccaacacg aaagcatcag cttctgctga acaaagttga gctcgatctc ctctcgtcgc
     4021 agcgactgaa tcagatctgc ggagacaaac agccactccg cgtattcgcg aagcaacgaa
     4081 gtaatttgct gatgcgcgga ttcgcgcaag tttggcgtcg tgaggtcttg cagcatgtga
     4141 ggtctgcttt cttatgtgct tttgtgcctt tttgtggcct acttctaccc tgtgctacat
     4201 ttttggtcaa cttatgaaga acgtttttct tgctggagca gtacgaacac cgatcggcaa
     4261 attcggcggc acgctcgcct catggactgc cgcggacctc ggcgtcgcag tcgcgaaaga
     4321 gagcctgcat cgcgccggac tgtcgccgga acaggtcact gattcgattt ggggttgcgc
     4381 gcgtcaggcc ggtggcggac caaacgtcgc gcgacaaatc acatttcgcg ccggcgtccc
     4441 ggaaacagtg cccgccgcga cgatcaatca ggcatgtggc tcgggattga aagcaataat
     4501 cctcgccgcg caggaaatca tgctcggtcg cgccgacgcc gttctggcag gtggcacaga
     4561 aagcatgtcg cgcgtgccgt atttcgccga tggtgctcgt tggggcatgc gcatgggaaa
     4621 catgcaactc gtcgacggca tgtatcgcga tggtttcagc gatccgcttt caggattgct
     4681 gatgggccaa acagctgaaa agctggcgcg gcaacatcag atctcgcgac aggaacaaga
     4741 tgagtttgcg ttgcgttcgc agcaaagagc cgaagcggcg atcgcgtccg agagattcaa
     4801 cgacgaagtg atgcctcttg aaatcgccgg caagaaagga acgacgagct tctcccagga
     4861 cgaacactgt cgcgccggcg ccacccttca ggacctcgcc aaactgccgc cagtcttcta
     4921 caaagacggc accgtcactg ccggcaattc ttccggcatc accgatggcg ccgctgcgct
     4981 cgtggtgttg agtgaagacg ggatcaaacg cttcggaacc acgccccagg ttcgcattgt
     5041 tgattatgag atcaccggcg ttgcgccgga catcatgggt atcggtcccg tgcctgcggt
     5101 ccgtgcatta ctcgagcgac aacatctgac tctgtcgcag atcgatctga tcgagctgaa
     5161 cgaagcgttc gcggcgcagg tgattgcctg cgatcgggaa ctcggattcg acgctgaacg
     5221 tttgaacgtc aacggcggct cgattgcgtt gggccatccg atcggctgta ccggagttcg
     5281 catcacgacg acgctcattt acgagatgca aaagcgacaa gcgaagctcg gtctcgccac
     5341 gctctgcgtc agcggtggta tgggactcgc gctcttgctg gaacgcgtat gaccgtgccg
     5401 ctaagaagtg cgctgattct gatcgcgctg gtcccgctca cgctttcctg tcgttcgcag
     5461 agtgataaga ctgcaaacaa caactcgaac aacgacacta tagtttcctc aacaccaccg
     5521 tttcagacgc gcgaacccga tcgctaccgc gccatccgga ccgtcacgat cgggtcgcca
     5581 aacggtcaga ccttaacaac caaaaacttg atcgcgcgcg atggcgactt gcgccgaaac
     5641 gaatctcaag tgagttcgaa acagattgcg tatctggaaa ctcccgaagg aagattcgtt
     5701 ttggtcccag cagaaaagat ctacgctgaa gttgctcctg gcgcagatct tccagccagc
     5761 gaatccgacg agacactaga gcgctctccc gaaggtttac tgcacgcaga aaccggcaac
     5821 acgagttatc agagactcgg cgaagaagcg ctcggagaac gcaggacaga caaataccgc
     5881 gtaattgtta acactgcgag tgcggcgagc gtcagcgtaa gtgagacgtt gatttggatc
     5941 gacaacgctc tgaagatgcc
//
LOCUS       DASSFQ010000270         3633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_275557, whole genome shotgun sequence.
ACCESSION   DASSFQ010000270 DASSFQ010000000
VERSION     DASSFQ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275557"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VFH96_09755"
     CDS             complement(<1..1020)
                     /locus_tag="VFH96_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET6974298.1"
                     /translation="MQFYFIEFSYLLASIFFILGLKGLSHPESAKRGMHLAEFGMLMA
                     IIGTLMHHEIITYTWILAGLAIGSVVGLAMGVWVPMTAMPQRTALSHAFGAFAAALVG
                     ISEFYRFGGHLENRVMMGALGFEVMLGALTTTGSLLAFAKLQGLVRGTPMTYKGQNVF
                     NMALLAVTIGIFVYLIVVPGSMPLFFTMVGLAFVFGLLLVMPIGSADMPVVMSLLNSY
                     AGLASAATGFVLSNKVLIIAGTLDGFSGFILSILMCRAMNRSMTNVLFGAFGSAATAA
                     AGGEQDGTMREVGLDDIATQLAYANKVVFVPGYGLATAQAQHAVRELADVLEKRGVTV
                     KYGIHP"
     gene            complement(1092..1415)
                     /locus_tag="VFH96_09760"
     CDS             complement(1092..1415)
                     /locus_tag="VFH96_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET6974299.1"
                     /translation="MPLDDPVVQKILLISALYVFALAAFLGYQVISRVPPLLHTPLMS
                     ATNAISGISLVGSIVTAGAQYNTVSTILGFIAVVCSTTNVVGGFVITDRMLKMFKRKE
                     IAKKE"
     gene            complement(1426..2568)
                     /locus_tag="VFH96_09765"
     CDS             complement(1426..2568)
                     /locus_tag="VFH96_09765"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HET6974300.1"
                     /translation="MNIVVLRETQAGEARVALMPESVKKLAGLGTSVQIESQAGLNAA
                     RTDDDYREAGAEVNSDREALLAAADVLAAVNRPSADDFGRLKKGAVVVGFLRPLDEPA
                     ALLPAVDRGVTTFSVELIPRITRAQAMDALSSMATVAGYKAVLIGAAHIPRMFPLLMT
                     AAGTVPPARVLVLGAGVAGLQAIATARRLGAVVEGYDVRAAAGEQVKSLGATFLEVDL
                     GGIKTEDAGGYAVELSDEGMNRGRALIAEHAKTADVIVTTAQVPGRRAPLLITEEAVN
                     GMKRGSIVIDLAGATGGNCALSQADVTVERNGVTIVAPTNLPATIPVHASQLYSRNVT
                     SFLSLLIKDGQLNIDMKDDVVGPSCVTHNGEVVNQRVAAALQAQPG"
     gene            2688..2761
                     /locus_tag="VFH96_09770"
     tRNA            2688..2761
                     /locus_tag="VFH96_09770"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2722..2724,aa:Glu,seq:ctc)
     gene            3025..>3633
                     /locus_tag="VFH96_09775"
     CDS             3025..>3633
                     /locus_tag="VFH96_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET6974301.1"
                     /translation="MIAVAAIGVFVSSYLDKTHALLNIRYHISEFLQGLNPRGEDAQR
                     TVLVVITDEEYWKELYGRVPIRRDYLAKIVRRAAELRVAVIALDFDLRSTSGGTPEPP
                     EFQKETIELRNAIYDAATRGIKVVLTRTLGQAADGAYMVEPDVYGDDVLSWPNISNGY
                     HVLPDDVRELPLVLSTANAGRLDSFALAIARADQAPSLRRIKE"
BASE COUNT          800 a          981 c         1044 g          808 t
ORIGIN      
        1 cggatgaatg ccgtatttga ctgtgacgcc gcgtttttcg agtacgtcgg caagttcgcg
       61 aaccgcgtgt tgtgcctgtg cggttgcgag cccatatccc ggcacgaaca cgactttgtt
      121 tgcgtaagcg agctgggtgg cgatgtcgtc aaggcccact tcgcgcatcg ttccgtcctg
      181 ctcgccgccg gctgccgccg ttgctgcgct tccgaacgcg ccgaacaaaa cgttcgtcat
      241 cgatcgattc atcgcgcgac acatgaggat cgagagtatg aatccggaaa aaccgtcgag
      301 cgtgccggcg atgatcagta ctttgttcga gagcacgaag cctgtcgcgg ccgaagcgag
      361 tccggcgtaa gaattcagca gcgacatcac aaccggcatg tcagccgatc cgattggcat
      421 caccagcaac agtccgaaga caaacgcgag acccaccatc gtgaaaaaca atggcatcga
      481 gccgggcacg acgatcagat acacgaagat tccgatcgtc accgcgagca gggccatgtt
      541 gaagacgttc tggcccttgt aagtcatcgg cgtgccgcgc acgagcccct ggagttttgc
      601 aaaggcgagc agactgccgg tggtcgtgag tgcgccgagc attacttcga aaccgagcgc
      661 gcccatcatg actctgtttt cgagatgacc accaaagcgg tagaactcgg agataccgac
      721 gagtgccgcg gcaaaagctc cgaacgcgtg tgagagcgca gttcgttgcg gcatcgcggt
      781 cattggcacc cacacgccca tcgcgagtcc gaccacagaa ccgatcgcga gacctgcgag
      841 tatccacgtg taagtgatga tctcgtgatg catcagcgtg ccgatgatgg ccatcaacat
      901 gccaaactcg gcgagatgca ttccgcgctt cgcgctttcg ggatggctca accccttcaa
      961 tccgaggatg aaaaagattg aagcgagcag gtagctgaat tcgatgaagt agaattgcat
     1021 aaattagcca cggattacac ggatgaacac ggctttaatt agatcggtgt ttatctgtgt
     1081 aatctgtggc tctattcctt cttcgcgatt tccttccgct tgaacatctt caacatgcga
     1141 tcggtgatga cgaagccgcc gacgacattc gtcgtggaac aaaccacagc gatgaacccg
     1201 aggatcgtgc tgacagtgtt gtactgcgca cccgcggtga cgatcgagcc gacgagagag
     1261 atgccggaga tcgcgttagt cgccgacatc agcggcgtgt gcagcagcgg cggcactcgc
     1321 gaaatcacct ggtaaccaag aaaagccgcg agcgcgaaca cataaagcgc cgaaatcaga
     1381 agtatttttt ggactaccgg atcgtctaag ggcattcgct tgctcctaac ctggttgcgc
     1441 ctgtagcgcc gccgcgacgc gttggtttac gacttcgccg ttatgcgtga cgcatgatgg
     1501 tccaacaacg tcgtccttca tgtctatatt tagctgtccg tctttgatca gcagcgagag
     1561 gaaggaagtg acgttgcgcg agtagagctg gctcgcgtgg accggaatcg tcgccggcaa
     1621 atttgtcggt gcgacgattg ttacgccatt gcgttcgacg gttacgtctg cctggctcag
     1681 tgcgcagttg ccgccggttg cgccggcgag gtcgatgacg atcgagccac gcttcatgcc
     1741 gttgactgct tcttcagtga tcagcaacgg agcacgtctg cctggcactt gtgcggtcgt
     1801 gacaattacg tcggcggtct ttgcgtgttc agcaatcaac gcgcgtccgc ggttcatccc
     1861 ttcgtccgag agctcgactg cgtagccacc cgcgtcttcg gtcttgatgc caccgagatc
     1921 gacttccaag aatgtcgcgc ctaatgattt cacctgctcg cctgcggctg cgcgcacgtc
     1981 gtagccttcg acgacggcgc cgagacggcg tgcagtcgca atcgcttgca gtccggcaac
     2041 gcccgcgccg agtacgagca cgcgtgccgg tggtacagtt cccgcggcag tcatcaacag
     2101 cggaaacatt cgcgggatgt gagcggcgcc gatcaggacg gctttataac cggcgacagt
     2161 ggccatcgac gagagcgcgt ccattgcctg tgcgcgtgtg attcgtggaa tcagctcgac
     2221 ggagaaagtc gtgacgccgc gatcgactgc gggtaacagc gcggcgggct cgtcgagtgg
     2281 gcgcaggaag ccgaccacga ctgcgccttt tttgagtcgt ccaaaatcgt ctgctgaagg
     2341 tcgattgact gcggcgagta catccgcggc ggcgaggagg gcttctcgat ctgagtttac
     2401 ttcggcgccg gcttcacgat aatcatcgtc agtgcgagcg gcgttaagac cggcttggct
     2461 ttcgatttgt acggaggttc cgagtccggc gagtttctta acggattcgg gcatgagcgc
     2521 gacgcgtgcc tcacccgctt gtgtctcccg caacacaacg atgttcatag gcgaaggtgc
     2581 atcgaggctt agttagctca aacgcgtgca gtatagcaaa taaggccaga tctgcgtcca
     2641 taacttgcta tcggcgcact gaggcaccta taatcccaga cttctgcggc gcattggtgt
     2701 agttggttaa cacgctgccc tctcaaggca gagatcacgg gttcgagtcc cgtatgcgct
     2761 acaaccaact tttaacagtt tataagccat ctcgctgttt aggcggggca gttccgctat
     2821 tgacaattcc caacctgcgg gaatatagtc cgcaactcat cggctgatta tctcacgagg
     2881 gtcagagctc tttagcttcc actaaaacaa acgtccaact cctcgttgcg atcagttgat
     2941 cgatatcctt gcgtccacat cacaagcaga ggaaaactta cagtgaaccg gatgtggtct
     3001 tttctccgaa gttattgggt gagggtgatt gcagtagccg caattggtgt atttgttagt
     3061 tcttatttgg ataagacgca cgctctatta aacatccgtt atcacatatc cgaatttctg
     3121 caagggctga acccgcgagg cgaggatgcc cagcggacag tgcttgttgt aatcaccgat
     3181 gaggaatact ggaaagaact ctacggaagg gtgccaattc ggcgcgacta ccttgccaag
     3241 attgtcagac gggcagctga acttagagtc gcagtaattg ctctcgattt tgatttgagg
     3301 tcgacgtcag gcggtactcc cgagcctcct gaatttcaga aggaaacaat agaactgcga
     3361 aatgcgatct acgatgctgc aacacgggga ataaaagtgg ttctgacgcg aacgcttgga
     3421 caagctgcag acggggccta catggtggag ccggatgtgt acggtgacga tgttttgtcg
     3481 tggccgaaca taagtaatgg ctatcacgtg ctgcctgatg atgtgcggga acttcctttg
     3541 gtattgtcga cggctaacgc cggtcgactg gattcctttg cacttgcaat tgctcgtgct
     3601 gatcaggctc cttcgttaag gcgaatcaag gaa
//
LOCUS       DASSFQ010000271         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1906397, whole genome shotgun sequence.
ACCESSION   DASSFQ010000271 DASSFQ010000000
VERSION     DASSFQ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906397"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFH96_09780"
     CDS             <1..388
                     /locus_tag="VFH96_09780"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET6974302.1"
                     /translation="IEIAMKARNCSLAPFSNFLVGAALRTEAGKIYTGCNIESASYGL
                     TVCAERVAIWKALSEGERQFTELAVVADTETLTPPCGTCRQIIWEFARGATIVFANLQ
                     GKSEVFQIADLLPRAFDARFLKKTTE"
     gene            complement(439..639)
                     /locus_tag="VFH96_09785"
     CDS             complement(439..639)
                     /locus_tag="VFH96_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974303.1"
                     /translation="MSEDLEPLIEAAKEARTHAVAPFSGFQVGAAVKTASGKVYTGCN
                     VESASYGLTVCAELARRRPVMG"
     gene            complement(636..1277)
                     /gene="udk"
                     /locus_tag="VFH96_09790"
     CDS             complement(636..1277)
                     /gene="udk"
                     /locus_tag="VFH96_09790"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET6974304.1"
                     /translation="MSLIIGICGGTGSGKTTVANRILESVSASEVVFIQQDSYYRNLT
                     DLPLDYRHIANFDHPDALDNDLLVNHVRRLKAGEAVELPLYDFKTHSRMPETRHVEPK
                     PIVIVEGILIYADPRLLEQMDIKVFVDTPDDIRFIRRLRRDLAERGRTVESVIEQYIA
                     TVRPMHMQFVEPSKRHADVIIPEGGHNLVSIDLLSGKIRERLASALTSVGGRL"
     gene            complement(1285..2139)
                     /locus_tag="VFH96_09795"
     CDS             complement(1285..2139)
                     /locus_tag="VFH96_09795"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET6974305.1"
                     /translation="MAEGVAGTSLYERAEHAARIVRARTKLEPRIAIVLGSGLGGFAD
                     EFDEAVGVPYEEIPGFSRSTAQGHAGRLVIGKVDEVPVMAMQGRVHYYEGYSLEQVTF
                     PIRTFKLLGVKTLILTNASGGINVQLSQGALMVISDHLNMMGDNPLRGPNDERFGPRF
                     PDMSAVYAPELQEIVIEEARAIGVEVRRGIYGALSGPSYETPSEIHLLRNLGADAVGM
                     STVPEAIVARHMGLEVLGISCITNMAAGISDEPINHEEVMATGDRVRDVFTLLLQRVV
                     GRVHSRVV"
     gene            complement(2149..>2547)
                     /locus_tag="VFH96_09800"
     CDS             complement(2149..>2547)
                     /locus_tag="VFH96_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974306.1"
                     /translation="AADAAGVSVSRLRYSGVLLAGMLAGVGGAYLSIGQSSLFTRNMT
                     AGRGFIALAALIFGKWRPVQTMLACFLFAFTDAISVQMQGAVKLPSGEDIPVQFINMV
                     PYVLTIVVLAGFIGSSRPPRALGVPYQKEK"
BASE COUNT          584 a          775 c          652 g          536 t
ORIGIN      
        1 aatcgagata gcgatgaagg cgcgtaactg ttcgctggcg ccgttttcaa actttctggt
       61 gggcgcagcg ttgcgcaccg aggccggcaa gatttacacc ggctgcaaca ttgaaagcgc
      121 gagctatggc ctgacagttt gtgccgagcg cgtggcgatc tggaaagcgt tgtcggaagg
      181 tgaacggcag tttaccgaac tcgcggttgt ggctgatacg gaaacgttga cgccgccgtg
      241 tgggacgtgt cgccagatca tctgggagtt tgcgcgcggc gcgacgattg tttttgcgaa
      301 tcttcagggc aagagtgagg tttttcagat cgccgacctg ctgccgcgcg cgtttgatgc
      361 gcgcttcctg aagaagacca ctgaatagtt catctatgcg tctgcttatt gttgtcgtca
      421 cattaatagc catgattgct aacccattac aggacgccga cgcgcaagct cggcgcaaac
      481 agtcaaacca taactcgcac tctccacatt gcagccggta taaacttttc cactcgctgt
      541 cttcaccgcc gcaccaactt gaaatccgga aaacggcgcc accgcgtggg ttcgcgcttc
      601 cttagccgct tcaatcaacg gctctaaatc ttcgctcaaa gtcgacctcc aacagaagtt
      661 aaagcgctgg ccagcctctc gcgaattttt ccgctcagca aatcaatgct caccagatta
      721 tggccacctt caggaatgat tacgtcggca tgacgtttcg acggttcgac aaactgcatg
      781 tgcatcggcc gcaccgtcgc gatgtattgt tcgattaccg attccaccgt gcgcccgcgt
      841 tccgccaggt cgcgccgcaa acggcgaatg aaacggatat cgtccggcgt gtcgacaaac
      901 actttgatat ccatctgctc gagcaaacgc ggatcggcat agatcaaaat gccttcgacg
      961 atgacgatcg gcttgggctc gacgtgccgc gtttcaggca tccgcgagtg cgtcttgaaa
     1021 tcgtaaagcg gcagttccac cgcttcgccc gctttcaaac ggcgcacgtg gttcacgagc
     1081 agatcgttat cgagcgcatc cggatgatcg aagttcgcga tgtggcgata gtcgagcgga
     1141 aggtcggtaa ggtttcggta gtaggagtct tgctgaataa aaactacttc gctggcactg
     1201 accgattcaa gaatgcgatt cgcaacagtt gttttccccg atcccgtgcc accacaaatg
     1261 ccgataatta aactcacgat tcacctacac cacgcgacta tgaactcgac ccacgactcg
     1321 ttgcagcaac aacgtaaaca cgtcgcgcac gcgatctccc gtcgccatca cttcttcatg
     1381 attgatcggc tcgtcgctga ttcccgcggc catgtttgtg atgcacgaaa tgccgagcac
     1441 ttccaggccc atgtgacgcg ccacgatcgc ctccggcacc gtgctcatac ccacggcatc
     1501 agcgccgaga ttgcgcaaca gatgaatctc agaaggcgtt tcgtaactcg gaccagacaa
     1561 agcgccgtaa attccgcgcc tgacttccac gccgatcgct cgcgcttcct cgatcacgat
     1621 ctcctgcaac tcaggcgcat aaacagcgga catgtcggga aaccgcggtc cgaaacgttc
     1681 gtcgtttgga ccacgcaacg gattgtcgcc catcatattc aagtggtcgc tgatcaccat
     1741 cagcgcgccc tgactgagct gcacgttgat cccaccggaa gcgttcgtca ggatcagcgt
     1801 cttgacgcca agcagtttga aagtgcgaat cggaaaggtg acttgttcga gagaatagcc
     1861 ttcgtagtag tgaacgcggc cctgcatcgc catgaccggc acttcatcaa ccttgccgat
     1921 caccaaacgg cccgcatgtc cctgcgcggt cgaacgggaa aacccgggaa tctcttcgta
     1981 cggaacaccg actgcttcgt cgaattcgtc ggcaaatccg cctaatccac ttccgagtac
     2041 gatcgcaatg cgcggctcca atttggtgcg cgcgcgcact atgcgcgcgg catgttcagc
     2101 tcgttcgtaa agactcgtgc ctgccactcc ctctgccatg accatgtgct acttctcttt
     2161 ctgataaggt actcccaaag cccgcggggg acgcgacgat ccaataaagc ctgccagcac
     2221 cacgatggtc aagacgtacg gcaccatatt gatgaactga accggaatat cttcgcccga
     2281 cggcaacttt accgcgccct gcatctgaac cgaaatcgca tcggtaaacg caaacaaaaa
     2341 acaagcgagc atcgtctgca ccggacgcca cttgccgaag atcaacgccg cgagcgcgat
     2401 gaatccacga cccgcagtca tgtttctcgt gaacagtgac gactgtccga tggatagata
     2461 cgcgccacca acgccggcca gcatgccggc gagcaacacg cccgaatatc tcaacctgct
     2521 gacactgacg cccgccgcgt cagccgc
//
LOCUS       DASSFQ010000272         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1906429, whole genome shotgun sequence.
ACCESSION   DASSFQ010000272 DASSFQ010000000
VERSION     DASSFQ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906429"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1860)
                     /locus_tag="VFH96_09805"
     CDS             complement(13..1860)
                     /locus_tag="VFH96_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974307.1"
                     /translation="MSLRSKLLTGYGIFIAALVALGAWSAWRLHDMGKVSRRIISENY
                     DSVVAAQEMKESLERQDSAALFVLLGARERGLAQLREHRARFDRSLDTAANNITELGE
                     SAAIAAIQRDREIYYQRFDAFIASSTRDEYFTQLEPQFNQLRADCEHLLQLNQRAMRF
                     KSEAAAGVAQLWFYRTLLIAGVLVVAGVVLAFWLAQRIVGPIRRLTESTVKIAGGDLN
                     ASVIVDSSDEVGVLAAEYNRMAETLRQLRSSDMGKLLVAQQTTEAAIDSLYDPVIVTD
                     GDGRVTKLNPAAEEIFGPEEKNTGMHVGEVARDVRIAGAVAEALESQRPVAAEEMSSV
                     LPLAVAGSERAFRLRTTPMRGNDHHLLGAVTLLEDITHLREIDRLKSEFIATASHELR
                     TPLTSVQMGVYLLLERAVGQLTEKQVEVLSACRDDCDRLDQLMRDLLDVSRIEAGESK
                     PSFEPLRMRDLLDAATKELQPQVEAKGLDFRVEAPVELSVVRVDRSQVERVLANLVVN
                     AIRYTKQGEIKISAQPRGNFVAVSVSDTGSGIPAEYLPHIFDKFVQVPGAATGGAGLG
                     LAISRLIVEAHGGQISVQSEPSRGSTFTFTLPITEPSAVAPGQLKNHAR"
     gene            complement(1886..3058)
                     /locus_tag="VFH96_09810"
     CDS             complement(1886..3058)
                     /locus_tag="VFH96_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6974308.1"
                     /translation="MLAELKKPSPAALLAKLKTGDKPTLRVYIGAAPGVGKTWQMLED
                     AHLLKRERVDIVIAVLETHGRFETAALAESIERVPMRKIDYRGVTLEEMDLEAVIARD
                     PQVAIVDELAHTDVPGSKNRKRYEDVLELLDHDISVITAVNIQHIESLNDVVARTTGV
                     RVRETVPDFFFRRADEVVNVDVSVDTLRTRLRQGKIYGVEKIEQSLNNFFRKGNLSAL
                     RELALRQVAQDQATRAHDYREQEGLDQAVIPEKVMVAIASHGSAKKLLRTGSRIAGRL
                     ASDWYAVYVETPREEMGRIKPEDYVALQENIQFAQQLGARIVRLKARNVADALIDFAR
                     REGITHVIFGQTSRSRWDILWHGSVINRFLDEVHDATIQVVPEESGATDNTDENRI"
     gene            complement(3048..3626)
                     /gene="kdpC"
                     /locus_tag="VFH96_09815"
     CDS             complement(3048..3626)
                     /gene="kdpC"
                     /locus_tag="VFH96_09815"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HET6974309.1"
                     /translation="MKRNLITAVLMTIVTTIILGLIYPLVVTGLAQVIFPDKANGQLI
                     RNAEGTVIGSRLIGQPFTSPGYFRSRPSAAGANGYDASGSGGSNLGPINQKLIDRVKA
                     DVEKLQAENPGKPVPIDLVTTSGSGLDPHLSPAAAEFQVPRVARERGMSEDELRQLVA
                     AHTEGRQFGFLGEPRVNVLELNLDLDNRNNAR"
     gene            complement(3712..>5301)
                     /gene="kdpB"
                     /locus_tag="VFH96_09820"
     CDS             complement(3712..>5301)
                     /gene="kdpB"
                     /locus_tag="VFH96_09820"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HET6974310.1"
                     /translation="DESAITGESAPVIREAGGDRSAVTGGTRVLSDQIKVRITSNPGE
                     TFIDRMIALVEGAERQKTPNEIALNILLAGLTIIFLLAVVTLQPFAIYSNAPQTIFVL
                     VSLLVCLIPTTIGGLLSAIGIAGMDRLIQHNVLAMSGRAVEAAGDVNTLLLDKTGTIT
                     LGNRQATQFVPLPGVNETELAEAAQLSSLADETPEGRSIVVLAKEKYGLRGRELAPHE
                     ANFIPFTAQTRMSGVDFDGREVRKGAADAIAKYLNGNGRAAPRELDGIVADISKAGGT
                     PLVVAEDRIALGVIYLKDVVKGGMAERFKQMRQMGIKTIMITGDNPLTAATIAREAGV
                     DDFLAQATPEDKMALIRREQEGGKLVAMTGDGTNDAPALAQADVGVAMNTGTQAAKEA
                     GNMVDLDSNPTKLIEVVEIGKQLLMTRGALTTFSISNDVAKYFAIIPAMFAGAFPVLN
                     ALNIMGLRTPQSAILSAVIFNALIIVALIPLALKGVKYRPMSAAALLRRNLLIYGLGG
                     IVVPFIGIKIIDVVINALRVV"
BASE COUNT         1091 a         1616 c         1437 g         1157 t
ORIGIN      
        1 gggtcaatct catcagcggg cgtggttttt aagttgaccc ggcgctaccg cgctcggttc
       61 tgttattggc aacgtgaacg taaacgtcga gccccggctc ggctccgatt gcacactgat
      121 ctgcccgccg tgcgcctcga cgatcagccg tgagattgcc aggcccaggc cagcacctcc
      181 ggtcgccgcg ccgggtactt gcacaaactt gtcgaagatg tgcggcagat actccgcggg
      241 aatgccgctg cccgtgtcgg aaacggaaac agcgacaaaa tttccacgcg gctgtgcgct
      301 gatctttatc tcgccctgtt tcgtgtaacg aattgcgttg acgacgaggt ttgcaagcac
      361 acgctcaacc tgcgagcgat cgactctcac caccgacagc tcgaccggcg cctcaactct
      421 gaaatcgagt cctttcgctt ccacttgcgg ttgcaattct tttgtggcgg cgtcgagcag
      481 atcacgcatc ctgagcggct cgaaactcgg tttactttca cccgcctcga tacgggagac
      541 gtcgagcagg tcgcgcatta actggtccag gcgatcgcag tcatcacgac aagcactcag
      601 cacctccact tgtttctcag tcaactgtcc tacggcgcgt tcgagcagca gatacacacc
      661 catctgtaca ctcgttaacg gagtgcgcaa ttcgtgcgat gcggtggcga taaattcaga
      721 cttgagacga tcgatctcgc gcaggtgagt gatgtcttcc agcaacgtaa cagcaccgag
      781 cagatgatga tcgttgccac gcatcggcgt ggtccgtagt cgaaacgctc tctccgaacc
      841 ggccacagcc agcggcaaca ctgacgacat ttcctcggct gcaaccggac gctgcgactc
      901 caaagcctct gcgactgcgc ccgcgatccg cacatcacgc gcgacctcgc cgacgtgcat
      961 tccggtgttc ttctcttccg gcccgaagat ctcttctgcc gcaggattta gtttggtgac
     1021 acgaccatca ccatccgtga cgatcacggg atcgtaaagt gaatcaatcg cagcctcagt
     1081 agtttgttga gcgacgagca acttgcccat gtcggagcta cgcagttgtc gcagcgtttc
     1141 cgccatccgg ttgtattcag cagcgaggac gccgacttcg tcactcgaat cgacaatcac
     1201 gctcgcgttg agatcgccgc cggcgatctt gaccgtgctc tcagtgagac ggcgtattgg
     1261 tccaacgatg cgctgggcca gccaaaacgc cagcacgacg cccgcgacca ccaacacgcc
     1321 cgcaatcaga agtgtgcgat agaaccagag ctgcgccaca cccgctgcgg cttctgattt
     1381 gaagcgcatc gcacgttgat tcaactgcaa caagtgttcg cagtcagcgc gcagttgatt
     1441 gaactgcggc tctagttgcg tgaagtattc gtcacgcgtt gagcttgcga tgaaggcatc
     1501 gaagcgctgg tagtaaattt cgcgatcgcg ctgaatcgca gcgatcgcgg cagactcacc
     1561 cagttcagtg atgttgtttg cagcggtatc aaggcttcga tcgaagcggg cccggtgctc
     1621 gcgcagttga gctaaacctc tttcacgcgc gccaagaagc acaaagagtg cggctgaatc
     1681 ctgccgctcc agactctctt tcatctcctg cgccgcaaca accgaatcgt aattctctga
     1741 aatgatccgc cgcgacacct ttcccatgtc gtgcaaacgc cacgcgctcc acgcccccaa
     1801 cgccacgagc gccgcgataa agatcccgta cccagttagc aacttggatc taagactcat
     1861 gtttttagcc agagatttca cggatttaaa tccgattttc atccgtgtta tccgtggccc
     1921 cgctttcctc cggcactact tgaatagtcg cgtcatggac ctcatccaaa aaacgattga
     1981 tcaccgaccc gtgccacaaa atgtcccaac gcgaacgcga cgtttgtccg aagatcacat
     2041 gcgtgattcc ttcgcgtcgc gcaaaatcaa ttagcgcgtc ggcaacgttg cgcgccttca
     2101 gccgcacgat acgcgcgccg agttgctgcg caaactgaat gttctcctgc aacgccacat
     2161 agtcctcggg cttgattcgt cccatctctt cgcgcggcgt ctccacgtaa accgcatacc
     2221 agtcagacgc gagtcgtccc gcgatacgcg aacccgtccg cagcagcttc ttcgcactgc
     2281 cgtgagaagc gatcgccacc ataaccttct ccggaatgac tgcctggtcc aaaccctcct
     2341 gctcgcgata atcgtgggct cgggttgcct ggtcctgggc cacctgccgc aacgcaagct
     2401 cgcgtaacgc cgacaagttt cccttgcgaa agaagttgtt gagcgactgc tcgatcttct
     2461 cgacgccgta gatctttccc tggcgcagcc gcgtccggag agtgtcgaca gaaacatcga
     2521 cgttcacgac ttcatcggcg cgacgaaaga aaaagtcagg gaccgtctcg cgtacacgca
     2581 cgcccgtcgt gcgagcgaca acgtcattga gtgattcgat gtgctggatg ttgacggcag
     2641 tgatgactga gatatcgtga tcgagaagct cgagcacatc ttcgtagcgc ttgcggtttt
     2701 tcgatccggg aacatccgta tgcgcgagct cgtcgacgat cgcgacctgc ggatcgcgcg
     2761 cgatgaccgc ctcgaggtcc atctcttcga gggtgacgcc gcgataatcg atcttgcgca
     2821 tcggcacacg ctcaatgctt tcagcgagcg cggcagtctc gaaacgaccg tgagtttcaa
     2881 ggacggcgat cacgatatcg acgcgctcgc gtttcaacag gtgcgcatct tcgagcattt
     2941 gccaggtctt gccgacaccc ggcgcggcgc cgatataaac gcgcagtgtc ggcttgtcac
     3001 cggttttcag tttggcgagc agcgccgcag gcgatggttt tttaagctca gcgagcatta
     3061 tttctgttgt cgagatcgag attgagctcg agcacgttca ctctcggttc accgagaaat
     3121 ccgaactgcc ggccttcggt gtgcgccgcg acgagctggc gcagctcgtc ttcactcatg
     3181 ccgcgctcac gtgcgacgcg tggcacttga aactccgccg cggcagggga gaggtgtgga
     3241 tcgagtcctg agccggaagt cgtgaccaga tcgattggca cgggcttgcc cggattttct
     3301 gcctgcagct tctcgacgtc agcctttact cgatcgatca acttctgatt aattggacca
     3361 aggttcgacc ctccagagcc actcgcgtcg taaccgttcg cgccggctgc cgacggtcgc
     3421 gagcggaaat aacccggcga agtaaatggc tggccgatga gacgcgaacc gatgactgtg
     3481 ccttcagcgt tccggatcaa ttggccgttt gctttgtccg gaaagatcac ttgtgcaaga
     3541 cccgtcacga cgagtggata aatcaacccg agaatgatcg tagtcacgat cgtcatcaaa
     3601 actgctgtga ttaaattcct cttcaacgcc tgctccttag agagttgtta gacaggattg
     3661 actagatttc caggatttac atgtccatcc tgaaaatctt gtcaatcctg tctaaaccac
     3721 ccgtaacgca ttaatgacca catcgatgat cttgatcccg atgaacggca ctacgatccc
     3781 gccgagcccg taaatcagca ggttacgtcg cagcagcgcc gccgcactca tcggtcgata
     3841 cttcacccct ttcaatgcca gtggtatcaa cgcgacgatg atcagcgcat tgaagatcac
     3901 ggccgagaga atcgccgact gcggcgtccg caatcccatg atgttgagtg cgttcagcac
     3961 cggaaacgcg cccgcaaaca tcgccggaat gatcgcgaag tacttcgcca catcattcga
     4021 aattgagaac gtggtcaacg cgccgcgcgt catcaacagt tgcttcccga tctcaacgac
     4081 ctcgatcagc ttcgtcgggt tgctgtcgag atcgaccatg ttgcccgctt ctttcgccgc
     4141 ctgcgtgccc gtgttcatcg cgaccccgac atcagcctgg gccaacgcag gcgcgtcgtt
     4201 ggtgccgtcg cccgtcatcg ccacgagctt gccgccttcc tgctcgcgcc tgatgagcgc
     4261 catcttgtct tcaggcgtcg cctgcgcgag aaagtcgtcg acaccggctt cgcgcgcgat
     4321 cgttgctgca gtgagtggat tgtcaccggt gatcatgatc gtcttgatgc ccatctgccg
     4381 catctgcttg aaacgctcag ccatgccgcc cttgaccacg tcttttagat agatgacgcc
     4441 gagcgcgatt cgatcctcag cgacgacgag cggcgttcca cctgccttcg agatgtctgc
     4501 gacgatccca tccagctcgc gcggcgctgc tcttccattt ccgttgagat acttcgcgat
     4561 cgcatccgcc gcgcctttgc gaacttcgcg accgtcgaaa tcaacgccgg acatgcgcgt
     4621 ctgtgcagtg aatgggatga agttcgcttc gtgcggcgca agctcgcggc cgcgcagtcc
     4681 atatttctct ttcgcgagga cgacgatcga gcggccttcg ggagtctcgt ctgcgagtga
     4741 cgaaagttgc gccgcttcgg caagctctgt ttcgttgacg ccgggtagcg gaacaaactg
     4801 tgttgcctgg cgattgccca acgtgatcgt gccggtcttg tcgagcagaa gcgtgttcac
     4861 gtctccggcg gcttcgactg cgcgtccgga cattgcgagc acgttgtgtt gaatcaggcg
     4921 gtccatgccc gcaatgccga tggctgagag tagtccgcca atcgtggttg gaatcaaaca
     4981 aacgagaagt gaaacgagca cgaagatcgt ttggggtgcg ttggaataga ttgcgaaagg
     5041 ctgaagcgtg accaccgcca acaggaaaat gattgtgaga ccggcgagca ggatgttcag
     5101 cgcgatttcg ttgggtgtct tttgtcgttc ggcgccctcg acgagtgcga tcatgcgatc
     5161 gataaatgtc tcaccgggat tcgaagtgat gcggaccttt atctgatcgg aaaggacgcg
     5221 cgtgccgcca gtgaccgcag agcgatctcc accggcttca cgaattaccg gcgcgctttc
     5281 acccgtgatt gccgactcat c
//
LOCUS       DASSFQ010000273         8950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1759243, whole genome shotgun sequence.
ACCESSION   DASSFQ010000273 DASSFQ010000000
VERSION     DASSFQ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759243"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFH96_09825"
     CDS             complement(<1..426)
                     /locus_tag="VFH96_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6974311.1"
                     /translation="MALYPLTRMSKEAPPNLLRGLSLLDATMIVVGSMIGSGIFITSA
                     ESSRLVGSPGWLLAAWALAGVLTITGALCCAELAAMWPRAGGQYVFLREAYGPSIGFL
                     FDWGLFLIIQSGTIAAVAVAFANFTGVLTDRVAGDRYLIA"
     gene            550..1884
                     /locus_tag="VFH96_09830"
     CDS             550..1884
                     /locus_tag="VFH96_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET6974312.1"
                     /translation="MKNDLINSITNKKAHVGVIGLGYVGLPLLVEFASWGFQGTGFEV
                     DDSKAEQINAGSSYIGDVPSERLKEVVDAKRLRATTDFSHLRECDAIIICVPTPLRKT
                     KEPDVSYILAAAEQIAKNLRRGHLIILESTTYPGTTDEVLLPMFEEKGLKLDEDFLLA
                     FSPERVDPGNPQFLTHNIPKVVGGVTEDSTAVAAHLYSQIVNDVHAVSSARVAEAAKL
                     LENTFRAVNIGMANEMARLCYALNIDTWEVIRAAATKPFGFMAFYPGPGIGGHCIPLD
                     PHYLSWKARQHGFDSRFIGLAEEVNSRMPEHVVQLVSDGLNDERKAMKGSNVLLLGVA
                     YKKDVNDVRESPALSIIDRLRAKGANVKYHDPFVADVHFDDAHTDSIGEPLSSVPLTD
                     DELRSSDCVIIVTDHSDIDYKRVCSTSSLIVDTRNALNGDTRRESKARIIRL"
     gene            1888..3000
                     /gene="wecB"
                     /locus_tag="VFH96_09835"
     CDS             1888..3000
                     /gene="wecB"
                     /locus_tag="VFH96_09835"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET6974313.1"
                     /translation="MKVINVVGARPNFMKVAPIVAAMKRRSHVFQPLVVHTGQHYDAA
                     MSDSFFRDLELPQPDVHLGVGSGSHAVQTAAVMERFEPVVLNEKPDWVLVVGDVNSTI
                     ACALVCVKLGVKVAHVEAGLRSRDRTMPEEINRLLTDQIADLLFTPSPDADENLIAEG
                     IPRERIRFVGNVMIDSLQKNLNVARAMPTQEQLGLNGIDYALLTLHRPSNVDARDSFE
                     RILDALEAISTRLPIVFPVHPRTRKTIAELGLSERVEAMKGLRLIDPLGYLDFLNLSS
                     NAHLVLTDSGGIQEETTALGIPCLTLRENTERPITVEMGTNIVVGTDTAKIIAAATSA
                     LNGSKKTTHRQPPLWDGHTSERILDALEEYAVHDSH"
     gene            3000..4862
                     /locus_tag="VFH96_09840"
     CDS             3000..4862
                     /locus_tag="VFH96_09840"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET6974314.1"
                     /translation="MAVLKKIKRAVRGEVKLTTVAREALRRTVASVNERKERAGVFVN
                     EPLRLKPAFARLSDAELLAHFQGPRDAKFFEPFPSTTDESIASANLGNPIQWTRDPLS
                     GYVWPLDYHRDINLRRSDGSDVRVLWELNRLGHFLPRASAYLTTNDERYAAEFFAQLK
                     SWTEQNPYARGPNWSCAMEVALRAINLLAAFEAFRHSQNLNAASLRSFLQLLQQHANY
                     IQRNLEFSHIATSNHYLSDVAGLTWLGLMLPELLDAKSWFEFGRTEMLREMDKQVLPD
                     GADFEASTGYHRFVTELFLYTFWLCRANGVKIEERYWTKLKQMLLYTRAYLRPDGFSP
                     LIGDTDGGQVLPFRQRRADDHAYLLEIGAVVFDDPTLADTQHLASQAFPQAGTYIMRD
                     GDLYLCFNASDAGLNGRGSHGHNDALSIEVSAGGHAFIVDPGTYVYTANLAQRHEFRS
                     TAYHSTVQIDGREQSTIDMQTPFVIGNEANPKVLEWTTNDDFDKVVAEHYGYAPITHR
                     RTVTFNKRDRHWLIDDEFLGDGEHVYEARFHFAPDVDVQVNGAAIDARAGDARLVISL
                     LNLQTEPVLENKPVSHDYGQMSDAISACWRISGRPGKLSWEIRFHAFAPLRETS"
     gene            4865..6787
                     /gene="asnB"
                     /locus_tag="VFH96_09845"
     CDS             4865..6787
                     /gene="asnB"
                     /locus_tag="VFH96_09845"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6974315.1"
                     /translation="MCGICGVWEYGASEGTVESSLLTRMRDEMTHRGPDDAGELIFDE
                     HRGGFGFRRLSIIDLSAAGHQPMHGCDDRTWLVFNGEIYNHALIRANLEHRGHKYASR
                     TDSETILHLYEERGLDFIHEIEGDYAIAIWDANKERLVLVRDRIGVKPLYFYHKDGRF
                     IFASEIKAILQHRSVTPDIDEQALYHYLTFLTTPAPTTLFRDIHKLPAGHILTLTRNG
                     TINVQRYWDALPPKQRVDGTDEEHRAEILRLLRESIRKRMMSDVPFGVFLSGGVDSSA
                     NVALMSEQMTRPVETFTVGFSDAEYLNELEPARRISRQFGTNHHEVIISEKEMHDFLP
                     SMVFHQDEPIADPVCVPLYYVSKLARDSGTIVVQVGEGSDEIFSGYENYVRHLRIYEN
                     FWRHAEALPLSLRRAISNISRPALEATGRKRAAIELIRRLGADEPLFWGGVVVYDETF
                     KPRVLSKQVRDRLNGLSSLRVVESYLKHIEQERPDADFLARMTYLELKLRLPELLLMR
                     VDKITMATSVEARVPFLDHHLVEYALSLPRSLKVEGVSGKHILKRSLEEILPRDLLYE
                     RKRGFGAPVREWFREGLGGWFDDHLMNSTMRRRDLLDYGFVSRMLDEHRRKTKDWGFH
                     LWALLNLSLWYERWID"
     gene            6869..8131
                     /locus_tag="VFH96_09850"
     CDS             6869..8131
                     /locus_tag="VFH96_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET6974316.1"
                     /translation="MKFSAQVAWTFATRVVMIFNSLAAGIIVAHWLGSEGVGQLAVIN
                     VAVATIVQLGSFGLPSSNTYFIAQDQARFRSAALNSLMFALGAGSILALALSAVASIR
                     PDWFGLDSVRLVQIAAISIPFQLLTLIGLNILLAVGKVRQFNILDLVSQSFVLINAAC
                     VWLLVKGNLGTLIVWNTAASVLVSLVIALLLVISAKSLAQSKWRADVALLRRMITYGL
                     KFHISILAGAIIIRADLLVVNHFRGSAEAGVYSVASQFALLLMLLPGVIATLLFPRVT
                     TEQDARGETTCLVTRYTAFLMFICCLAAVPFSLLLPLVYGAQFYDATGLLWILLPGVY
                     LMGLESVLVQHFNALGLPKAIPIYWLVTLAINLVLVFALVPRYGAQGAAIASTISYAA
                     IFGLVAFHFHSSTGRSFGEVFVLGGLRG"
     gene            8128..>8950
                     /locus_tag="VFH96_09855"
     CDS             8128..>8950
                     /locus_tag="VFH96_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974317.1"
                     /translation="MKVVLLALSGDLSRWQDKITELYPGASIEFISRTEFETGSHLKR
                     LKALRSLRPDVLAIATERLPWQRGQNLFMLFGALAGAREVLMIDAHGGLVKKSRSNVL
                     LGAPVRLSRETITGASDVAQSRRELQRLEEETLRFSDLPRKTTSRTRLRAVYLRSTPG
                     PGTQAGGAASHIKGVVEGLESLGVDVRIISNDEIAGLDAAEDRFEVIPPQPGGGSRAL
                     FDMHNNLVFTRGAVPLIERADPDFIYQRYARFSWAGVVAANRMKRPLLLEYNGSEV"
BASE COUNT         1880 a         2434 c         2574 g         2062 t
ORIGIN      
        1 cgcgatcagg tatcgatctc cggcaacgcg atcggtgagt acgccggtga agtttgcgaa
       61 cgcgaccgcg accgccgcaa tcgttcccga ttgaataatc agaaacaaac cccagtcgaa
      121 gagaaatcca atcgacggtc catacgcttc gcgcaggaaa acgtattgcc cgccggcacg
      181 cggccacatc gcggccagtt ccgcacaaca gagcgccccg gtgatggtaa gcacgcccgc
      241 cagcgcccag gcggccagca gccagccagg cgatccgacg agccgcgacg attcagcgga
      301 agtgataaaa atgccggagc cgatcatcga gcccactacg atcattgttg catcgagcag
      361 gcttagacca cgcagcagat ttgggggagc ttctttgctc attcgcgtga gcgggtatag
      421 tgccatagaa ctatccacag attacacaga ttttgttttt atgaaatctg cgaaatctgt
      481 ggatgaaacc cctgcttgac actgatcgtc aggctcacct agtcttttcg gtttggagtt
      541 tgtcacagga tgaaaaacga cctaatcaat tcaatcacaa acaaaaaagc gcacgtcggg
      601 gttatcggtc tcggatacgt cggtctccca ttactcgtcg aatttgcgtc ctggggtttt
      661 cagggcacgg gtttcgaagt tgacgactcg aaggcggaac agattaacgc cggcagttct
      721 tacatcggcg acgtgccttc ggagcgacta aaggaagtgg tcgacgcaaa gcgtctgcgc
      781 gccacgaccg acttcagtca cctgcgcgag tgcgacgcga tcatcatctg cgtaccgacg
      841 ccgctacgaa agacgaagga accggacgtc tcttacattc tcgcggccgc cgaacagatc
      901 gcgaagaacc tgcggcgggg ccatctgatc attctcgagt cgaccacata tccgggcacc
      961 acagacgaag tattgctgcc gatgtttgaa gaaaagggat tgaagctcga cgaagatttc
     1021 ctgctggcgt tctcgcccga acgagtcgat cccggcaatc cgcagtttct gacgcacaat
     1081 attcccaagg tcgttggcgg cgtgactgaa gactcaacag ccgtcgccgc gcatctttat
     1141 tcacagattg tgaacgacgt gcacgcagta agttcggcgc gtgttgctga agccgcgaaa
     1201 ctgctcgaaa acacatttcg tgcggtgaac atcggcatgg cgaacgagat ggcgcggctt
     1261 tgttacgcgc tcaacatcga cacctgggag gtgattcgcg ctgcggcgac caagccgttc
     1321 ggatttatgg cgttctatcc cggaccggga attggcggac attgcatccc gctcgatccg
     1381 cattatcttt cgtggaaagc acgccagcac ggattcgatt cgcggttcat cggactggcg
     1441 gaagaagtca attcacgcat gcccgagcac gtggtccaac tcgtttcgga cggactgaat
     1501 gacgaacgca aggcgatgaa gggttcaaat gttttgttgc tcggcgttgc gtacaagaaa
     1561 gacgttaacg acgtgcgcga gagtccggcg ctgtcgatta tcgatcgatt acgagcgaaa
     1621 ggcgcgaacg tcaagtatca cgatccgttc gtcgcggacg ttcatttcga cgacgcgcac
     1681 acggactcga ttggggagcc gttgtcgtcg gttccgttga ctgatgatga actgagatcc
     1741 tccgactgcg tcataattgt gaccgatcat agcgacatcg actacaagcg cgtttgctcg
     1801 acgagctcgc tgatcgtcga cacgcgcaac gcgctcaatg gcgataccag acgagagagc
     1861 aaagcccgca ttattcggtt gtaaatgatg aaagtcatca atgttgtcgg ggcgcggccc
     1921 aactttatga aggtggcgcc gatcgtggcc gcaatgaaga ggcgttctca cgtcttccag
     1981 ccgctggtgg tccacacggg acagcattat gacgcggcca tgtctgactc gtttttccgc
     2041 gatctcgaac ttccgcaacc ggatgtgcat ctcggtgtcg gctcaggctc gcatgcagtg
     2101 caaactgctg cggtaatgga gcggtttgag ccggttgtcc ttaacgaaaa gccggattgg
     2161 gtgttggtcg tcggcgacgt caactcgacg atcgcgtgtg ctctcgtgtg tgtgaagctg
     2221 ggtgtaaagg tggctcacgt cgaggcggga ctgcgaagtc gcgatcgcac gatgcccgaa
     2281 gagatcaaca gattactgac cgatcagatc gcggatttgc tgtttactcc ttcgcccgac
     2341 gccgatgaga atctcattgc cgaaggcatt ccgcgcgagc gaattcgttt cgtcggcaac
     2401 gtcatgatcg attcgctaca aaagaacctg aatgtcgcgc gtgccatgcc gacgcaagag
     2461 cagcttggat tgaacggcat cgattatgcg ctgctgactc tgcaccgccc gtcgaatgtc
     2521 gatgcgcgcg actcgtttga gcgcattctc gacgcgctcg aagcgatctc aacgcggcta
     2581 ccgatcgtct tcccggttca tccgcgcacg cgaaagacta ttgctgaact cggattaagc
     2641 gaacgtgtcg aggcgatgaa gggactgcga ctaatcgatc cgcttggcta tctcgacttc
     2701 ctcaatcttt cgagcaatgc gcatctggtt ttgactgact ccggcggcat tcaggaagag
     2761 acaaccgctc tcggcatccc ttgcctcacg ctgcgcgaga acactgagcg acccatcacg
     2821 gtcgagatgg gtaccaacat cgtcgtcggc accgacaccg cgaagatcat tgctgccgca
     2881 accagcgcct tgaacggttc caaaaaaacc acacaccgcc agcctccgct ttgggatgga
     2941 cacacctccg agcgtattct cgatgcgctc gaggagtatg ccgtgcacga ttcccattga
     3001 tggctgttct taaaaaaatc aaacgagcgg tccgtggaga agtgaagttg acgaccgtag
     3061 cgcgcgaagc gctgcgacgc actgtagctt cagtcaatga gcgcaaggag cgggctggtg
     3121 ttttcgtcaa cgaaccgctg agattgaagc ctgcgttcgc gcgcctgagt gatgcggaat
     3181 tgctggctca ctttcaaggt ccacgcgacg caaagttttt cgaacctttt ccaagcacaa
     3241 cggacgagtc gatcgcttca gcaaatctcg gcaacccaat tcagtggacg cgagatccgc
     3301 tctcaggcta tgtctggccg ctcgattacc atcgcgatat caacttgagg cgctcagatg
     3361 gatcagacgt gcgcgtgctc tgggaactga accggttggg tcatttcctt cctcgggcga
     3421 gtgcttactt gaccacgaat gacgagcgct atgcggctga gttcttcgca cagttaaaga
     3481 gctggaccga gcaaaaccct tacgcgcgtg gtccaaattg gagttgcgcg atggaagttg
     3541 cgctgcgcgc gataaacctg ctggctgcgt ttgaagcatt tcgtcattcc cagaatctga
     3601 atgccgcgtc tttgcggtcg tttctgcaac tgctgcaaca acacgctaat tacattcaac
     3661 gtaatctcga attctcccac atcgccacca gcaatcacta tctttcggat gtggctgggc
     3721 tgacctggtt gggattgatg ctgccggaac ttcttgacgc gaagtcgtgg tttgagttcg
     3781 ggcgaacgga gatgctccgc gagatggaca aacaggtgtt gcctgatgga gccgatttcg
     3841 aggcttcaac cggctatcac cggttcgtta cggagctgtt tctttacacg ttctggttgt
     3901 gtcgtgcgaa cggcgtgaag attgaggaga gatattggac caaactcaag cagatgctgc
     3961 tctacacgcg cgcctatctg cgtcctgacg gattctcccc gttgatcggc gacacagacg
     4021 gtggacaggt cttacccttc agacagcgcc gcgcagatga tcacgcgtat cttctcgaaa
     4081 tcggtgccgt tgtttttgac gacccaacgc tcgccgatac tcagcactta gcttcgcaag
     4141 cctttccgca agccggcact tacatcatgc gcgacggcga tttgtatctc tgcttcaacg
     4201 cgagtgacgc cggactcaac ggacgtggtt cgcacgggca caacgacgcg ctgagtattg
     4261 aagtctcggc cggcggccac gcatttatcg tcgatcccgg aacgtacgtt tacactgcga
     4321 atctcgccca gcgtcacgag tttcgttcga ccgcctatca ttcaaccgta cagattgacg
     4381 gtcgcgagca gagcacgatc gacatgcaaa cgcccttcgt catcgggaac gaagcgaatc
     4441 cgaaagtact ggagtggact accaacgacg attttgacaa ggtagtcgcg gaacattatg
     4501 gctacgcgcc aatcacgcat cgccgcacgg ttacattcaa caagcgcgac cgtcattggt
     4561 taatagacga cgaatttctc ggcgacggcg agcatgtata cgaagcgagg tttcatttcg
     4621 cgccggatgt cgacgtgcag gtaaacggag cggcaattga cgcacgcgcc ggcgatgcac
     4681 gcctggtgat ttcgttattg aacctgcaaa ctgaaccagt tttggagaac aagcccgttt
     4741 cacacgatta tggtcagatg agcgacgcga tctccgcgtg ctggcgcatc tcaggccggc
     4801 caggcaagct aagttgggag ataaggtttc acgcctttgc gcctttgcgt gaaacatctt
     4861 aatcatgtgt ggcatctgtg gagtttggga atacggcgcg agcgaaggaa cggttgagtc
     4921 ctcgctgctg acgcgcatgc gcgatgagat gactcaccgc ggtccggacg acgcgggcga
     4981 gttgatcttt gacgagcatc gcggcggttt cggcttccga cggctttcaa ttattgatct
     5041 ttcggctgcg ggtcatcagc cgatgcacgg ctgcgacgat cgcacgtggc tcgttttcaa
     5101 cggcgagatc tacaatcatg cgctcatccg cgcgaacctc gaacatcgcg gtcacaagta
     5161 cgcatcacgc accgattccg aaacaatcct gcatctctac gaagaacgtg gcctcgattt
     5221 cattcacgag atcgaaggcg actatgcgat cgcaatctgg gatgcgaaca aagaacgtct
     5281 cgtcctcgtg cgcgaccgca tcggcgtgaa gccgctctac ttctatcaca aagacggtcg
     5341 cttcatcttt gcttcggaga tcaaggcaat cctgcaacac aggtctgtca cgcctgacat
     5401 cgacgaacaa gcgctttatc actatctgac gtttctcacc acgcccgcac cgacgacgct
     5461 ctttcgcgac attcacaaac tgcccgctgg tcacatcctg actttgacgc gcaacggcac
     5521 gatcaacgtt cagcgttact gggacgcgct gccgcccaag cagcgagtcg acggcacgga
     5581 cgaggaacat cgcgcggaga tcctgcgcct gctgcgcgaa tcgattcgca agcgaatgat
     5641 gtcagacgtg ccgttcggag tttttctttc cggtggcgtt gattcatccg cgaacgtcgc
     5701 gctcatgtcg gaacagatga cgcgcccggt cgagaccttt actgtcggtt tcagcgatgc
     5761 cgagtatctc aacgagctcg agcccgcgcg tcgcatctcg cgacagtttg gcaccaacca
     5821 tcacgaggtg atcatctccg agaaagagat gcacgatttt cttccgagca tggtctttca
     5881 tcaggacgag ccgattgccg atccagtttg cgtgccgctc tattacgttt cgaagctcgc
     5941 gcgtgactcg ggaacgatcg tggtccaggt cggagaaggt tcggacgaga tctttagcgg
     6001 ttacgagaac tacgtgcgac acctgcgcat atacgaaaac ttctggcgac acgcagaagc
     6061 gctgccgctt tcgttgcgac gcgcaatttc gaacatttcg cggccggcgc tcgaagccac
     6121 gggcaggaaa cgtgccgcaa ttgaattgat ccggcggttg ggtgctgatg agccgttgtt
     6181 ctggggcggc gtggttgttt atgacgagac gttcaaaccg cgcgtgctgt cgaagcaagt
     6241 gcgagatcga ttgaatggac tgtcgtcatt gcgagtggtg gaatcgtacc tgaagcatat
     6301 cgagcaggag cggccggacg cagatttcct ggcgcggatg acttatctcg agttgaagct
     6361 gcgtttgccg gaactgctgt tgatgcgggt ggataagatc acgatggcga cgagtgtcga
     6421 agcgcgcgtg ccgtttctcg atcatcatct ggttgagtat gcgctttcgt tgccgcgctc
     6481 gctgaaggtt gaaggcgtga gtgggaagca catcctgaaa cgttcacttg aggagatcct
     6541 gccgcgcgat ttgctttacg aacggaagcg aggtttcggg gcgccggtgc gtgaatggtt
     6601 ccgagagggt ttgggtggct ggttcgacga ccacctgatg aactcgacga tgcggcgtcg
     6661 cgatctgttg gactacgggt ttgtgagccg catgttggat gagcatcgac ggaagaccaa
     6721 ggattggggt tttcatctct gggcgctgtt gaatttgagc ttgtggtacg agcgatggat
     6781 cgattagtaa gccgcggatt tacgcagata aacgcggata aaattcatat cggcgttcat
     6841 ccgcgtaaat ccgcggctaa aagatgaaat gaagttttca gcccaagttg cctggacgtt
     6901 tgcgacgcgc gtggtaatga tttttaacag cctcgccgcc ggcattatcg tcgcgcattg
     6961 gttgggatct gagggtgttg gccagttggc ggtgattaat gtcgctgtgg caacgatcgt
     7021 gcagctcgga agttttggtc tgccgtcttc aaacacttat ttcatcgcac aggatcaggc
     7081 acgcttccgt tcagccgcac taaattcgtt gatgtttgca ctcggcgccg gttcaattct
     7141 ggccctcgcg ctcagcgcgg tcgcatccat tcggcctgat tggtttggtc tcgactccgt
     7201 ccgtctcgtt cagatcgcgg ccatttccat tccgtttcaa ctgctcacgt tgattggact
     7261 gaacatctta ttggcggtgg gcaaggtgcg tcagttcaac atcctcgacc tcgttagcca
     7321 atcgtttgtt ctaattaacg ccgcgtgcgt gtggcttttg gtcaaaggca atttgggaac
     7381 gctgatcgtg tggaacacag cggcgagcgt tctggtgagt ctcgtgatcg cgctgttgct
     7441 ggtgatttca gcaaagagcc tggcgcaatc gaagtggcgt gcagatgtcg cacttctgcg
     7501 ccggatgatt acctacggtc tcaagtttca tatttcgatt cttgccggcg cgatcatcat
     7561 tcgcgccgat ttgctcgtcg taaatcactt tcgcggatct gcggaagccg gtgtttacag
     7621 tgtcgcatca cagtttgctc tgttgctcat gttgttgccg ggcgtgattg caaccttgct
     7681 atttccgcgt gtgacgaccg aacaggacgc gcgtggtgaa accacctgcc tcgtcacgcg
     7741 ctacacggcg ttcctcatgt tcatctgctg ccttgctgcg gttccgttta gtctgctgct
     7801 gccgctggtt tatggcgccc agttctacga cgccaccggg ctgctttgga tcctgctgcc
     7861 gggtgtttat ctgatgggac tcgaatcggt gctggtccaa cacttcaatg cgctcggtct
     7921 cccgaaagca attccgatct actggcttgt cacgctcgcg attaatctcg tgcttgtatt
     7981 tgcgctcgtg ccgcgttatg gcgcgcaggg cgcagcgatc gcttcaacca tcagttacgc
     8041 tgcgatcttc ggacttgtgg cctttcactt tcacagtagc actggaagat ctttcggaga
     8101 ggtgttcgtg ctcgggggtc tgcgcggatg aaggtcgttc tgctcgcgct cagcggcgat
     8161 ttgtcgcgct ggcaagacaa gatcacggag ctttatcccg gcgcttcgat cgagtttatc
     8221 tcgcgcaccg aattcgaaac cggcagtcac ctcaagcgac tcaaggcgtt gcgttcgctg
     8281 cgtcctgacg tgctcgcaat tgcaactgag cggcttccct ggcaacgcgg tcaaaatctt
     8341 ttcatgctct tcggcgcact tgcgggcgcg cgcgaagtgt tgatgatcga cgcgcatggt
     8401 ggactcgtaa agaagtctcg ttcgaatgta ctgttgggtg cgccggtgag attgagtcgt
     8461 gagacgatca cgggtgcgag cgatgtcgcg caatcgcggc gcgagctgca acgtcttgaa
     8521 gaagagacac tgcgattcag tgatttacct cgtaagacga cttctcgcac tcgcttgcgt
     8581 gcggtttatc tgcggtcgac tcccggaccg ggcacacagg caggtggcgc tgccagccac
     8641 atcaaggggg ttgtcgaagg tctggagtcg cttggcgtcg acgttcggat catcagtaac
     8701 gacgaaatcg ccggcctgga tgcggcggaa gatcgcttcg aagtgatacc gccgcaaccg
     8761 ggcggtggct cgcgcgcact cttcgacatg cataacaatc tggtgtttac acgtggcgcg
     8821 gtgccgctca tcgagcgtgc cgatcccgat tttatctacc agcgttacgc gcggtttagc
     8881 tgggctggag tggtcgctgc gaaccgaatg aagcgcccgt tgttgctgga atacaacggt
     8941 tctgaggtgt
//
LOCUS       DASSFQ010000274        21324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_437147, whole genome shotgun sequence.
ACCESSION   DASSFQ010000274 DASSFQ010000000
VERSION     DASSFQ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437147"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /gene="dxs"
                     /locus_tag="VFH96_09860"
     CDS             <1..1622
                     /gene="dxs"
                     /locus_tag="VFH96_09860"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET6974318.1"
                     /translation="RRDESEYDTFGAGHASTSLSAALGMALARDHKGEDHHVVALIGD
                     ASLAGGMAMEAINQAGHLKSRLIVVLNDNEMSIAPAVGALTGYLNRIRGAHGYHRFKD
                     EVEGKLLAIPSVGERLHQAAKTMKDMIAAAVLPGALVNELGFKYIGYVDGHNPRALVA
                     AFEEAKQIKDGPVIVHALTTKGKGFPQTEPDYYRWHATGPFDLQTGKPHKSSSKIPTY
                     TTVFGNTLAELMEKDPKVVALTAAMPDGTGVCTALEKFPDRSFDVGIAEQHCVTFAAG
                     MSCEGLKPVCAIYSTFLQRGFDQVIHDVCIQDLNVKFCLDRGGIAGGDGQTHHGLLDI
                     AYLRPIPNIVVMAPKDEGEMRDMMFTMLEHNGPVAMRYPRGNGVGVPVDREPQLLEIG
                     KGEILRDGGEVAIVAYGAMVHPSLAAADRLAKDRIETTVVNARFVKPLDTPLLLALAR
                     TKRLIVTVEEAYLAGGFGSAVMELLEENGLQDQVRVVRMGIPDRLITHGDAKLLLAKY
                     GLDADGIYTRVRENIEVLDDRRLATKGIRKN"
     gene            1834..2418
                     /locus_tag="VFH96_09865"
     CDS             1834..2418
                     /locus_tag="VFH96_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974319.1"
                     /translation="MKDYSEPHFDDERTILRARRVVPLEKIEAKVRHRRQWFLGTAFA
                     LAMMLGAGAALIASYVKLRQAPQAATEVAVEPEGESAPVAVVESTSSPTPTPEQQVAE
                     TTPPVIARKEPKRRPVPAPYDPEPVDDRNVRQANEAEQLQQIRDAVLYDEWQERRARR
                     VQRRERRRADGYNHRDLSNLDEIFEGRRRRSDRP"
     gene            2627..2878
                     /locus_tag="VFH96_09870"
     CDS             2627..2878
                     /locus_tag="VFH96_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974320.1"
                     /translation="MKRQMFRERECLHREEGANGDFYNGVFYLQALQRLRVDEAVKMA
                     GKVTSFFWSDAPQIIVWLCDDCAAELSLSETPRALTRRA"
     gene            2906..3649
                     /locus_tag="VFH96_09875"
     CDS             2906..3649
                     /locus_tag="VFH96_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET6974321.1"
                     /translation="MKRERIDKLLVERGLAESRTKAQAMVMAGVVLVDEQRVEKPSHQ
                     FDPNSSIRVKGGDDPTSRYVGRGGLKLEAALREFQIEVAGLTCLDVGASTGGFTDCLL
                     QHGANGVVAIDVGHNQIDWRLRNDPRVEVREGVNARYLTPEAFPSKFDLAVMDVSFIS
                     ATKVLPAIVPLLIDGGTIVTLIKPQFEVGRGEVGGGGVVRDAAKRARVIEEVNAAARA
                     LGLEVVNVIESPIAGAEGNIEFLAQYKLM"
     gene            3711..4589
                     /locus_tag="VFH96_09880"
     CDS             3711..4589
                     /locus_tag="VFH96_09880"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET6974322.1"
                     /translation="MSSIKRIGIVLKPHQPQALKTICELVVWLAERGITLVGGPDLER
                     ERIEHETGCPVPQVEHDNLAAEVDLMLVLGGDGTMIATARTIGDQEVPVLGVNYGGLG
                     YLAEFRIEELYSALESILAGNYRLDRRVMLAVEHCHGDDPPNTYRVLNDVVINKSALA
                     RIIEIEAYLNSQFVNSFRADGLIVSTPTGSTAYNLSAGGPVIFPSMNAVVITPICPFT
                     LSNRPIVVPDDAEIELLLKTDKEDVTLTLDGQVGFPLEIADRVRIRKSRTTFNLIQPS
                     NRNYFDVLRDKLRWGR"
     gene            4602..6020
                     /locus_tag="VFH96_09885"
     CDS             4602..6020
                     /locus_tag="VFH96_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HET6974323.1"
                     /translation="MPDKRDNPNLIERDAFEREVGTDLDEETPDKPKGIPLHTKILIG
                     LALGVMAGVSVNEVLGGDHPKVVWIVDNITQPVGQLFLRLLLMIVVPLVFSSLVVGVA
                     GIGDIRKLGRVGIKSFGYCLVISAISAVIGITLANTIKPGKRIDPNTAAALQQRYAND
                     ATKSVEAAKQASSATPKPLMAFVETIVPKNPFYAVAGKEDANMLHLMFFALMLGIAIT
                     LIPETVTGPVLRVLEGVYEITAKIIEIIMKFAPYAVACLLFNNTARFGLDLLQALAWF
                     VATVLIGLALHMFGVYSISVYLLSRISPLEFFRRIKTVMLTAFSTSSSNATLPTALRI
                     SEQNLGVPPQINSFVLTVGATANQNGTALYEGVTVLFLAQLAGVNLSLGQQLMVVYLA
                     ILGGIGTAGIPSGSIPFIIGVLVTIGVNPALIAIILGVDRILDMCRTTLNVTGDITAA
                     TYVARSEGYELLKPHETVLAAD"
     gene            6051..6800
                     /locus_tag="VFH96_09890"
     CDS             6051..6800
                     /locus_tag="VFH96_09890"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6974324.1"
                     /translation="MVLPLWDDNTDRQTTPIVNYAIIALNIFVFVVLQGLGSNDQFTY
                     SFSTVPAEILQGRDIVTPAQTVEYAGQLVRIPGLGRTPGSVYLTLLTSMFMHGSIMHI
                     AGNMLFLWIFGDNIEDRLGHVRYLIFYLLCGVIASLAHVFTTGAFASSEALLVPSLGA
                     SGAISGVLGGYLLLHPRRRVTVILFRFLTDVPAYVAIGIWFAFQLISGLGILGGGSQA
                     GGVAYAAHVGGFIAGLVLIKFFDRGTRQPAY"
     gene            6890..7300
                     /gene="queF"
                     /locus_tag="VFH96_09895"
     CDS             6890..7300
                     /gene="queF"
                     /locus_tag="VFH96_09895"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET6974325.1"
                     /translation="MDVLNIQSTSRAEMNLFPLDTFAYEFPGKEIKINFEITEFTAIC
                     PFSDFPDFATIRIEYVPNERCIELKSLKLYINSFRDVKIFHEHVINIILEDFVAACDP
                     IRVDIEGDFHVRGNIKTIVRASYTHPQITQKNNL"
     gene            7297..8532
                     /locus_tag="VFH96_09900"
     CDS             7297..8532
                     /locus_tag="VFH96_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6974326.1"
                     /translation="MKSADTTLWRRYRGLPRNVFAIGLVSLLNDASSEIIYPLLPIFL
                     AGSLGASARAIGTIEGLAESISSLLKLFAGYLSDRLEKRKFLVVAGYSVASIARPLLA
                     FAQTWTQVLAIRLTDRIGKGIRTAPRDAMIADTVRVEQRGIAFGFHRAMDHAGAVIGP
                     LIGYLLVILFVANAKSPTTGEFSKIFLAASVPALIAVLVAVFFMRESPRQAAHGSEIK
                     KLSLRGFDSNFKRFLLVLALFTLSNSSDSFLILRAMDSGVSIAVIPLLWAAHHGMKVL
                     SSLLGGDLSDRLGRKRLIVAGWILYAAVYAGFAFATHQASLWILFLVYGIYFGLAEGA
                     EKALVADLVRPEQRGTAYGLYNLAFGITVFPASFLMGMIWDWKGPTVAFLVSAFMGAM
                     AAMLLLIFVRPHPVLQERI"
     gene            8534..9055
                     /locus_tag="VFH96_09905"
     CDS             8534..9055
                     /locus_tag="VFH96_09905"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6974327.1"
                     /translation="MRRRVLTPHPGLATGIGYYLSGMEEVRAQVVAAVKTIPDDLIGK
                     PAFLGAHSIGGLVLHIGEAEWFWMQMVVSGHKLTEEDKQAPYWDVLDDIDGVARNAYT
                     TEFCLKEIDLIRNQTRDVLFSYNDKDLERIISFERNGETTEYSLRWILHRLIDHEAQH
                     KGQIFMLKRLMAL"
     gene            9142..10911
                     /locus_tag="VFH96_09910"
     CDS             9142..10911
                     /locus_tag="VFH96_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET6974328.1"
                     /translation="MNYELIKSFDELRKAIEILATQPVVGLDTETTNLDPYTSRLRLI
                     QLATADRVYIVDFDHFANGDANESLGPLRRLLEAPRPIKIAHNAKFDAKFIKHNLGVD
                     LGGLFDTLLASQLVGAGDIEERHGLETVASRYLNEAVDKSERLSNWSFELSEAQLEYA
                     ARDAAVLLPLREKLIDRLKSESLVRVAQLEFECVMPVVDIELAGFYMHKERWLEQLSI
                     VEKRRVELAEQLQQVLAEESSQGSLFGGPQREDINLDSQQQLTKALTRLGIPVPDSTR
                     NWKLQPLAVEYPIIGTLLEYRTVQKALTSYGENMIGMINPVTNRLHADFRQIGAPTGR
                     FACTNPNIQQVPHSVEYRRCFSGYPDGRKLIIADYSQIELRILAEFSGDRGFIEAFNS
                     GADLHRVTAAQVFNASLDQVTKEQRDFAKRLNFGVVYGIGAQRFALMTGLTVPDAENV
                     LRRYFATYRQLDTYLRDAANRAVSERQARTASGRLVRFRYDENDRQQISMTQRNGKNT
                     PIQGTSADILKRALKLLRDELRETNAKVVNIIHDEIVVESDADEAQDIAEKVERVMRV
                     AGEEYLKTVPVKVETEIADEWLK"
     gene            complement(10937..12457)
                     /locus_tag="VFH96_09915"
     CDS             complement(10937..12457)
                     /locus_tag="VFH96_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6974329.1"
                     /translation="MATESAPKLVRGLSLLDATMIVVGSMIGSGIFITSAESSRLVGS
                     PGWLLVAWALAGVLTITGALCCAELAAMWPRAGGQYVFLREAYGPSIGFLFGWGLFLI
                     IQTGTIAAVAVAFANFTGVLTDRVAGDRYLIAPIVLLKPFTINQLSFNGYALSLSSQQ
                     LLAVVMILFLTFINTRGLQTGKIIQNTFTFTKTAALLGLILIGLIVGWNHGSAAFTSS
                     WWAPWENGWKPSASQVWFEPVGEGLLVLALLMMLGRAMVGPLFAQSAWNNVTFTAGEV
                     RDPGKNLPRALLLGCGLVVTFYLLANLAYVVTLPLAGIQNAPQGRVATAVMQAVFGPR
                     GTVIMALAIMVSTFGCNNGLILAGARVYYAMARDGLFFKRVDSVNEFHVPAVALIAQG
                     LWASFLVLPRTVTVNQQAGTYTFGNVYTQLLEYIVSADLIFYVLMVGAVIVMRRKAPE
                     LERPYRTVGYPFVPLLYIVIAGLFIIDLAILAPSTSGIGYLLVFTGIPIYFIWRRR"
     gene            12547..13881
                     /locus_tag="VFH96_09920"
     CDS             12547..13881
                     /locus_tag="VFH96_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET6974330.1"
                     /translation="MKKDLIALITNKQAHIGVIGLGYVGLPLLVEFTSRGFHGTGFEV
                     DEAKAQQINAGTSYIGDVSSRKVKDVVDAKRLRATTDFSHLSECDAIIICVPTPLRKT
                     KEPDVSYILAAAEQIAERLRRGHLIILESTTYPGTTDEVLLPMFEAKGLKLDEDFLLA
                     FSPERVDPGNPQFQTHNIPKVVGGVTDDSTVVAAHLYSQIVNDVHAVSSARVAEAAKL
                     LENTFRAVNIGMANEMARLCYTLNIDTWEVIRAAATKPFGFMPFYPGPGIGGHCIPLD
                     PHYLSWKARQHGFDSRFISLAEEVNSRMPEHVVQLVADGLNDARKAMNGSNVLLLGVA
                     YKKDINDVRESPALSIIDRLRAKGANVRYHDPFVAELRFDDAHTDSSGEPLSSVSLTN
                     DELQSADCVIIVTDHSDIDYKRICSIVPLIVDTRNALNGDIRRESSARIIRL"
     gene            14007..15104
                     /gene="wecB"
                     /locus_tag="VFH96_09925"
     CDS             14007..15104
                     /gene="wecB"
                     /locus_tag="VFH96_09925"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET6974331.1"
                     /translation="MLKVINVVGARPNFMKVAPIVAAMKKRPDTFLPLVVHTGQHYDA
                     AMSDSFFRDLDLPQPDTHLGVGSGSHAAQTAAVMERFEPVVLREKPDWVLVVGDVNST
                     IACALVCVKLGIKVAHVEAGLRSRDRTMPEEINRLLTDQIADLLFTPSHDADENLIAE
                     GIPKERICFVGNVMIDSLQRNLAVARALPTHEQLGIDGDYALLTLHRPSNVDVRDSFD
                     RILTALEVITEKLPIVFPVHPRTRKTITELGLSERVDAIKDLRITEPLGYLDFLNLSS
                     SACLVLTDSGGIQEETTALGIPCLTLRENTERPITVEMGTNVIVGTDTTKIIAAAKAT
                     LNGSAKKPVVRPPLWDGHTSERILDALEQSR"
     gene            15104..16885
                     /locus_tag="VFH96_09930"
     CDS             15104..16885
                     /locus_tag="VFH96_09930"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET6974332.1"
                     /translation="MPVLNKIKRALRGEVKLTTAAREALRRGRVSLQQRKERAHLGES
                     EPLALRQPFARMSADELSGYFRGPRQAKFFDHSWPLLDFDEQALGKEIQWRRDPLSNY
                     VWPLDYHRDIKLMRTDGSDIRVLWELNRLGHFLPLACAYASTHDERYSAEFVTQLKSW
                     HEQNPYGRGPNWTCAMEVALRAMNLLAAFEVFRQSPQMPVEFFLQLLQQHGNYIRRNL
                     EFSYLATSNHYLSDVVGLLWLGLLLPELRDANAWRDFGLDQMLREMDKQVLADGADFE
                     ASTGYHRFVTELFLYSFLLCRTNNVEIPKRYWSKLEQMLLYIRAYLRPDGFAPLIGDT
                     DGGQVLPFVRRRADDHAYLLDILKLETEHVGSRAFPHAGTYIMRDGDLYLCFNASGEG
                     INGRGSHGHNDVLSIEVSAGGRAIIVDPGTYVYSADLAKRHEFRSTAYHSTVQIDGLE
                     QNTIRVDTPFVIGNEAKPRVLEWKTSDDFDKVVGEHYGYPVTHRRTVTFDKRARLWVI
                     DDEFIGDGEHVYEARFHFAPGLDVRVNGPAVEAGSLVVTLLGSDIQPVLQTQSASIDY
                     GQMSDSISACWKISGKPRKLTWQIKSV"
     gene            16903..18825
                     /gene="asnB"
                     /locus_tag="VFH96_09935"
     CDS             16903..18825
                     /gene="asnB"
                     /locus_tag="VFH96_09935"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6974333.1"
                     /translation="MCGICGVWEYGASDGSIESSLLARMRDEMTHRGPDDAGDLIFDN
                     HRGGFGFRRLSIIDLSAAGHQPMHGCDKRTWLVFNGEIYNHALIRADLERRGHKYSSR
                     TDSETILHLYEERGLDFIHEIEGDYGIAIWDANKERLVLVRDRVGVKPLYFYHKDGRF
                     IFASEIKAILQHPAVTPDIDEQSLYHYLTFLTTPAPATLFRDIHKLPAGHVLTLTRDG
                     TLNIQQYWDALPPKQKIAGTEEEQRAEILRLLRESIRKRMMSDVPFGVFLSGGVDSSA
                     NVALMSEQMSRPVETFTVGFSDAEYLNELEPARRIAKQFKTNHHEVIVSEKEMHDFLP
                     SMVFHQDEPIADPVCVPLYYVSKLARDSGTIVVQVGEGSDEIFSGYENYVRHLRIYEN
                     FWRHAEVLPLSLRRAISNLSRPALEATGRKRAAIELIRRLGADEPLFWGGVVVYDETF
                     KPRVLSQSVRERLNGLSSLRVIEKYLKHIEQERPNADFLARMTYLELKLRLPELLLMR
                     VDKITMATSVEARVPFLDHHLVEYALGLARTLKVEGTSGKHILKRSLEEILPRDLLYE
                     RKRGFGAPVREWFREGLGGWFDDHLINSSMRRRDLLDYGFVSQMLEEHRAKTRDWGFH
                     LWALLNLSLWYERWID"
     gene            18914..20128
                     /locus_tag="VFH96_09940"
     CDS             18914..20128
                     /locus_tag="VFH96_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET6974334.1"
                     /translation="MIFNSVVAGIIVAWWLGAEGVGQLAVINVAVATVVQIGSFGLPS
                     SNTYFIAQDQSHFRAAAINSLIFALGIGAVLAFALTAVASIKPDWFGFVSPQLVRIAA
                     VSIPFQLITLIGLNILLAVGKIREFNLLDLAGQSFVLINAALVLLLLKRGLEMLVTFN
                     TLASILVASVITLLLVISAKSLTQSKWRADIALLRRMIVYGLKFHISILAGAIIIRAD
                     LLVVNHFRGAAEAGVYSVASQFALLLMLLPGVIATLLFPRVTSEQDVRGETTCLVSRY
                     TTLVMFACCLAAVPFSFLLPFIYGPAFADATKLLLILLPGVYLIGLESVLVQHFNALG
                     LPRAIPIYWLVTLILNLVLVFTLVPRYGAQGAAIASSISYAMIFALVALHFHTSTGRS
                     FAEVFVLGSLRG"
     gene            20125..>21324
                     /locus_tag="VFH96_09945"
     CDS             20125..>21324
                     /locus_tag="VFH96_09945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6974335.1"
                     /translation="MRVVLLALSGDLDRWRDKLLALYPDSSIDLISRAEFEKGSHIAR
                     LRSLRALSPDVLAIATERLPWQRGQNLFMVFGALAGAREVLMLDAHGEMIRKSRSKVL
                     LSAPARLSRETIASAGDIARSRRELQQLEETTLRFVDMPRKITPRSRVRVVYLRSTPG
                     PGTQAGGAASHIKGVVEGLEALGAEVQIISNDLIAGMNAADDRFTVIPPQPGGGTRAL
                     FDIHNNLVFTRGAVPLIEQADPDFIYQRYARFSWAGVVAANRTKRPLFLEYNGSEVWV
                     GRYWDRVGRLELLERYERLNLDAAARIFVVSDVERRNLEARGVAAEKIVVNPNGVDVE
                     RFRPGVGGAGARRELGIRDDEVVAGFVGTFGPWHGVEKLAEAIKTIPANEKVRFLFVG
                     SGSLHAEV"
BASE COUNT         4555 a         5894 c         6096 g         4779 t
ORIGIN      
        1 tgcgccgcga cgaatctgag tacgacacat tcggcgccgg acacgcttcc acatcgctct
       61 ctgcggccct cggcatggcc ctcgcacgcg accacaaagg cgaagatcat cacgtagtcg
      121 cgctgatcgg cgacgcatca ctcgcgggcg gcatggcgat ggaagccatc aaccaggccg
      181 gccacttgaa gagccgtttg atcgtcgtac taaacgacaa cgagatgtcg atcgcgcctg
      241 ccgtcggcgc gctcacgggt tatctcaacc gcattcgcgg cgctcacggg tatcaccgtt
      301 tcaaagacga agtcgaaggg aagctgctcg cgattccctc ggtcggcgag cgactacatc
      361 aggcagcgaa gacgatgaaa gatatgatcg ctgccgccgt attacccgga gccctcgtca
      421 acgagctcgg tttcaagtac atcggctacg tcgacggcca caatccgcgc gcgctcgtcg
      481 ctgcgttcga agaagccaaa cagatcaaag acggtccagt gatcgtccac gcgctgacga
      541 cgaagggcaa aggctttccg cagaccgagc ccgattacta ccgctggcac gcgaccggcc
      601 cattcgatct ccaaaccgga aaaccgcaca agagctcgtc aaagattccg acctacacga
      661 ccgtcttcgg caacacgcta gccgagctga tggaaaagga tccgaaagtc gtggcgctga
      721 ctgccgcgat gcctgacggc accggtgttt gcacagcgct cgagaagttt cccgatcgtt
      781 ctttcgacgt gggtatcgct gaacaacact gcgtcacgtt tgcggccggc atgtcgtgtg
      841 aaggattgaa gcccgtctgc gcgatctact cgactttcct gcaacgcggt ttcgatcagg
      901 tgatacacga cgtctgtatc caggatctga atgtgaagtt ctgtctcgat cgtggcggta
      961 tcgccggcgg cgatggacaa acgcatcatg gcctgctcga catcgcgtac ctgcgtccga
     1021 ttccaaacat cgtggtgatg gcgccgaagg atgaaggcga gatgcgcgac atgatgttta
     1081 cgatgctgga gcacaatgga ccggtggcga tgcgttaccc gcgcggcaac ggcgtcggtg
     1141 ttccggtcga tcgcgagccg cagctactcg aaatcggaaa aggcgagata ctgcgcgatg
     1201 gtggtgaagt tgcgatcgtt gcgtacggcg cgatggtcca tccatcactc gcggccgctg
     1261 accgcctcgc aaaagatcga atcgagacga ctgttgtgaa cgcgcgcttc gtgaagccac
     1321 tcgacacgcc cttgctgctt gcgctcgcgc gcacgaagcg attgatcgtc acagttgaag
     1381 aagcatatct ggccggtggt tttggttcgg cggtgatgga actgctcgaa gagaacggtt
     1441 tgcaggacca ggttcgcgtg gttcgcatgg gcatccccga ccgcctcatc acgcacggcg
     1501 acgcaaagtt gctgctggca aagtatggcc tcgatgctga cggcatttac acgcgcgtgc
     1561 gtgagaatat cgaagtactc gacgatcgcc gcttggccac aaaaggcata agaaagaatt
     1621 agccacaaaa agcacaaaag gcacaaaatg aaatgccctt ttttgtgcct tttgtgcttt
     1681 ttgtggctaa ctgttttctg tgccttttcg tggcatggtt tggttttagt gcacgaaatc
     1741 ggaaggtttt agtgcttctc cccgccctaa tctccccaaa accagtcgct ttccgtgtgg
     1801 cctcgtgatt gcaaactgga tcgaggtttt acgatgaagg attactcaga gccacatttt
     1861 gatgacgagc ggactattct ccgggcgcgt cgtgtagtgc ctttggagaa gattgaggcg
     1921 aaggtgcggc accgcaggca gtggtttctc ggtacggctt tcgcgctcgc gatgatgctg
     1981 ggcgccggag cagcgctgat cgcgtcgtat gtgaaactgc gccaggctcc tcaagctgca
     2041 actgaggttg cagtcgaacc agaaggggag tccgcaccgg ttgctgtagt cgagagtaca
     2101 tcgagcccca ctccaactcc cgaacagcaa gttgctgaaa ctacaccgcc agtaattgcg
     2161 agaaaagaac caaagcgcag accagttcct gcgccatacg atcccgagcc cgtagacgat
     2221 cgtaacgtgc gacaggcgaa tgaagcagaa cagctacaac aaattcgcga tgcagtgctt
     2281 tacgacgagt ggcaggagcg acgtgcacga cgtgtgcagc gtcgcgagcg tcgccgggca
     2341 gatggttaca atcatcgcga tctttccaat ctcgacgaaa tcttcgaagg tcgtcgccgc
     2401 cgttcagatc gtccataaaa cggtagtggt tccgtttgtc gcgacagtcc gacaagctaa
     2461 agcttatcgg acatcaaact gtattgacgg aattgtgatc gaatggcatc atcacatcgc
     2521 tgcgatcgtc attgtcgtcg ccctgattta actcacacat tttaataacc cgacatttta
     2581 ataaactgat ctttttccgc gtgatgcaag cgcgttggtg aacatcatga agaggcagat
     2641 gtttcgtgag cgggagtgtc ttcaccgtga ggagggagct aacggtgatt tctacaatgg
     2701 ggtgttctac ctgcaagcct tgcagcgact gcgggttgac gaggctgtga agatggcggg
     2761 taaagtgacg tcgtttttct ggtctgatgc gccgcagatc attgtttggt tgtgtgatga
     2821 ttgcgcggct gagctcagct tgagcgagac gccgagggct ttgacgagac gggcatagac
     2881 agggttcgca ggatatatta atcctgtgaa gcgcgagcga atagacaaac tgcttgtcga
     2941 gcgaggtctc gcagagtcgc gcacgaaagc gcaggcgatg gtcatggcgg gcgtcgtgct
     3001 tgtcgacgag cagcgcgttg aaaaaccttc ccaccagttc gatccaaact catcaattcg
     3061 tgtcaagggc ggtgatgatc cgacgagtcg ttacgttgga cgcggcggac tgaagctcga
     3121 agcagcacta cgcgagtttc agatcgaggt cgctggactg acgtgtctcg acgtcggcgc
     3181 gtcgaccggc gggtttactg attgtctgct gcaacacggt gcgaatggcg ttgtggctat
     3241 agatgtcggt cacaatcaga tcgattggag actgcgaaac gatccccggg ttgaagtgcg
     3301 tgaaggcgtc aacgcgcgct acctgacgcc ggaagctttc ccgagcaagt ttgatctggc
     3361 ggtgatggac gtgtcgttca tctctgcgac gaaggtgttg cccgcgatcg tgccgttgct
     3421 tattgacgga ggaacgatcg tgaccttgat caagccgcag ttcgaagttg ggcgcggcga
     3481 ggttggtggc ggcggtgtcg tgcgtgacgc agcaaagcgg gcgcgcgtca ttgaagaagt
     3541 gaacgccgca gcacgcgcgc tcgggctcga ggtcgtgaat gtgatcgagt cgccgatcgc
     3601 aggcgcagaa gggaatatcg agttcctcgc acagtataag cttatgtaac ttttgtgctt
     3661 ttttgtggct taaactatca tcctgctgtt tttaacaccc tcgtcaaacc atgtcgtcaa
     3721 tcaagcggat aggcatagtc cttaaaccgc atcaacctca ggcactgaag accatctgcg
     3781 agctggtcgt gtggcttgcg gagcgcggca tcacgctcgt cggtggtcca gacctcgaac
     3841 gcgagcggat cgaacacgag acgggctgcc cggtgccgca ggtcgagcac gacaacctcg
     3901 ccgccgaagt cgatctgatg ctcgtgctgg gcggcgacgg cactatgatc gccaccgcgc
     3961 gcacgattgg cgaccaggaa gtgcctgtgc tgggcgtcaa ctatggcgga ctcggctatc
     4021 tggcggagtt tcgcatcgag gagctctaca gcgccctcga gtcgatcctc gcgggaaact
     4081 accgtctcga tcgacgcgtc atgcttgcag tcgagcactg ccatggagac gatccaccga
     4141 acacataccg cgtattgaac gacgtcgtca tcaacaaatc ggcactcgcg cgcatcatcg
     4201 agatcgaagc ttatctcaac agccagttcg tcaactcgtt tcgggccgac ggtctgatcg
     4261 tctcaacacc gaccggctcg actgcttaca atctttccgc cggtggacca gttatctttc
     4321 cctcgatgaa tgctgttgtc ataactccga tctgcccgtt tacgctttcg aatcgcccga
     4381 tcgtagtgcc tgacgacgcc gagatcgagt tgttactgaa aacagacaag gaagacgtga
     4441 cactgacgct cgacgggcag gttggttttc cattggagat tgcggaccgc gtcagaattc
     4501 gcaagagccg cacgacgttc aatctcattc agccaagcaa tcgaaactac ttcgacgttc
     4561 tgcgcgacaa gttgcgctgg gggcgttaat ctcaaataca catgcctgat aaaagagaca
     4621 acccaaacct aatcgagcga gatgcgttcg aacgtgaagt aggaaccgat cttgatgaag
     4681 agacgccgga taagccgaaa ggcattccgc ttcacaccaa gatcctgatc ggtctcgcgc
     4741 tcggcgtgat ggccggagtt tcggtaaatg aagtcctcgg cggcgatcat ccgaaagttg
     4801 tctggattgt cgacaacatc acgcagccgg tgggccaact attcctgcgc ttgctgctga
     4861 tgattgtagt gccgctcgtg ttttcttcac tcgtggtcgg cgtggcgggc atcggcgaca
     4921 ttcgcaaact cgggcgtgtg ggtatcaagt cgttcggtta ttgcctcgtg atttccgcga
     4981 tctcagccgt aatcggcatc acgctcgcca acacgatcaa gcccggcaag cgcatcgatc
     5041 ccaacaccgc agccgcattg caacaacgtt acgcgaacga cgcaacgaag agcgtcgaag
     5101 cggcgaaaca ggccagcagc gcgacaccaa agccgttgat ggcattcgtt gagacgatag
     5161 ttccaaagaa cccgttctat gcggtcgccg gcaaagaaga tgcaaacatg ctgcacctca
     5221 tgttcttcgc gctcatgctc ggcatcgcga tcacgctgat tccggaaaca gtcaccggtc
     5281 cggtgctgcg cgtgctcgaa ggtgtttacg agatcacggc gaagatcatc gagatcatca
     5341 tgaagtttgc gccgtatgcc gtcgcgtgcc tgttattcaa caacaccgcg cgtttcggcc
     5401 tggacctgct gcaagcgctc gcgtggtttg tcgcgactgt gctaatcggc ctggccctgc
     5461 acatgtttgg tgtttactcg atatcggttt atttgctgtc gcggatctcg ccgctcgaat
     5521 ttttccgccg cataaagacc gtgatgctga ctgcgttttc gacgtcgtca tcgaatgcga
     5581 ccctcccgac agcattgcga atttcagaac aaaacctcgg cgtgccgccg cagatcaata
     5641 gtttcgtgtt aactgtcggt gcgactgcga atcaaaacgg gaccgcgttg tacgaaggcg
     5701 tgacggtgct gttcctggcc cagctcgcgg gcgtcaatct ttcgttgggc caacagctaa
     5761 tggtcgtcta tctcgcgata ctcggcggca tcggcacggc gggtattccg tcgggctcga
     5821 ttccctttat cattggggtg ctcgtgacga tcggtgtgaa ccccgcgctc attgcgatca
     5881 ttctcggcgt ggatagaata ctcgacatgt gtcgcacgac cttgaatgtc acgggcgata
     5941 tcaccgccgc gacttacgtc gcgcgttcgg aaggctacga attattgaag ccgcacgaga
     6001 cagttttagc cgcggattaa ccgagattaa cgcagatctg aggttctatc atggttcttc
     6061 cactctggga cgacaatact gacaggcaaa cgacgccgat tgttaactat gcgatcatcg
     6121 cgctgaatat ttttgtcttc gttgtcttgc aggggcttgg cagtaacgat cagttcacgt
     6181 attcgttttc caccgtgccg gcggagattc ttcagggccg cgatatcgtc actccggcac
     6241 agacagtcga gtacgcgggg cagctcgtgc ggattcccgg tctcggccgg acgcctggtt
     6301 cggtttacct gacgctgctc acttcgatgt tcatgcatgg cagcatcatg cacatcgccg
     6361 gaaacatgct cttcctctgg atctttggtg acaacatcga ggatcgtttg ggccacgtga
     6421 gatatttgat cttttatttg ttatgcggcg tgattgcttc gttggcccat gtgtttacca
     6481 ccggtgcttt tgcgtcgagt gaagccttgt tggttccaag tcttggggct tcgggcgcga
     6541 tctcgggcgt gctcggcggt tatcttctgt tgcatccaag acggcgcgtg acggtgatcc
     6601 ttttccgatt tttgactgac gtaccggcgt atgtcgcgat cgggatctgg tttgcgtttc
     6661 agttgatcag cgggctcggt attcttggcg gcggatcgca ggcaggcggc gttgcgtacg
     6721 cggcgcacgt cggcggcttc atcgcgggac tggtgctgat caaattcttc gatcgcggta
     6781 ccaggcagcc ggcgtattag ccacggataa cacggattat aaaaacggat ttaaatccgt
     6841 catccatccg tgtagatctg tggccggatt ttgttaagat tcacgcctca tggatgtatt
     6901 gaatattcaa tcgacctcgc gagcggagat gaatctgttt ccgctcgaca cgttcgcgta
     6961 cgagtttccc ggcaaagaga tcaagatcaa tttcgagatc accgagttca cggcgatctg
     7021 cccgttctct gattttcctg acttcgccac tatacgcatc gaatatgttc ccaacgagcg
     7081 ctgtatcgag ttgaaaagtc tgaagctgta tatcaactcg tttcgcgacg ttaagatctt
     7141 tcacgagcac gtcatcaaca tcattctcga agacttcgtc gccgcctgcg atccaatccg
     7201 ggtcgacatc gaaggcgatt tccacgtccg cggcaacatc aagaccatcg tcagggcgag
     7261 ctatacacat ccgcagatta cacagaagaa caatctgtga aatctgcgga tacaacgttg
     7321 tggcggcgtt atcgcgggtt gccgcgcaac gtgtttgcga tcggtttggt gagcctgctc
     7381 aacgatgcgt cgagcgagat catctacccg ctgctgccga tctttctggc gggcagcttg
     7441 ggtgcttcgg cacgggcgat cggcacgatt gaaggactcg ccgagtcgat ctcgagcctg
     7501 ctaaaactct tcgccggtta tctcagtgat cgtttagaga agcgcaagtt tctggttgtt
     7561 gccggttatt cggttgcctc gattgccagg ccgctgctcg cgtttgcgca aacgtggacg
     7621 caggtgctcg cgattagact cacggatcgc atcggcaagg gaatcagaac ggcgccacgt
     7681 gatgcgatga tcgccgacac ggtgcgggtc gagcagcgcg gcattgcgtt tggatttcat
     7741 cgtgcgatgg accacgcggg cgctgttatc ggtccgctca tcgggtatct gctcgtcatc
     7801 cttttcgtcg cgaacgccaa atctccgact accggagagt tctcgaagat ctttctggcg
     7861 gcgtctgtgc cggcgttgat cgcggtgctg gtcgctgtct ttttcatgcg cgagtcgccg
     7921 cgacaggcag cgcacggatc tgagatcaag aagctttcgc tgcgcggctt tgacagtaac
     7981 ttcaagcgct ttctactcgt gttggcgctc ttcacgcttt ccaactcgtc ggacagtttc
     8041 ttgatcctgc gagcgatgga ttcaggcgtg tcaatcgcag tgatcccgtt attgtgggcg
     8101 gcgcatcacg gcatgaaggt gttgagctcg ttattgggcg gcgatctctc ggatcgtctc
     8161 gggcgcaagc gattgatcgt ggcgggatgg attttgtatg ctgcagttta cgcggggttt
     8221 gctttcgcga cgcaccaggc ttcactctgg atcctgttcc tcgtctatgg catctacttc
     8281 ggtctcgcag aaggcgctga gaaagcgctc gtggctgatt tggtgcgtcc ggaacaacgc
     8341 ggaacagctt acggcctgta caatctcgcc ttcggaataa cggtttttcc ggcatcgttt
     8401 ctgatgggta tgatttggga ctggaaggga ccgacagtcg catttctcgt cagcgcgttc
     8461 atgggcgcga tggccgcgat gctgctgctc atctttgtga gaccgcatcc ggtgttacag
     8521 gaaaggattt gagatgagac gccgagtgtt aacaccacat ccgggcttag caacaggaat
     8581 cggttactac ctcagcggca tggaggaagt gcgcgcccag gtcgtcgcgg cagtgaagac
     8641 cattcccgac gatttgatcg gcaagcctgc gtttctcggc gcgcactcga ttggcggtct
     8701 cgtgctgcac atcggcgaag cggagtggtt ctggatgcag atggtcgtca gcggccacaa
     8761 gctcaccgaa gaagacaagc aggcaccata ctgggacgtg ctcgacgaca ttgacggcgt
     8821 agcgcgcaac gcttacacga ctgagttttg tttgaaggag atcgatctga ttcgcaacca
     8881 aacgcgcgac gtgctcttct catacaacga caaggatctc gagcgcatca tctcatttga
     8941 gcgaaatggt gagacgacgg aatacagcct gcgctggatt ctgcaccgtt tgatcgatca
     9001 cgaagcccag cacaaagggc agattttcat gttgaagcga ctgatggcac tataggtcat
     9061 ccacagatta cacagattac acagatattt tgaaagttct gtgtaatctg tggatggttt
     9121 tacagagccc tatatttgtt gatgaattac gagctaatca agtccttcga tgagcttcga
     9181 aaagcaatcg aaatcctcgc aacgcaaccg gtggtcggtc tcgataccga aaccacgaac
     9241 ctcgatccgt acacctctcg cttacgactg atccagctcg ctaccgcgga tcgcgtctac
     9301 atcgtcgact tcgatcactt cgctaacggt gatgcgaatg aatcactcgg acctttgcgc
     9361 cggctgctcg aagcaccgcg cccgatcaag atcgcgcaca acgcaaaatt cgacgcgaag
     9421 tttatcaagc acaatctcgg cgtcgatctc ggtgggctgt tcgacacgct gctcgccagc
     9481 caactcgtcg gtgcgggcga catcgaagag cgccacggtc tcgagacggt cgccagccgc
     9541 tatttgaacg aagcggtcga caagtcagag cgcttgagca actggagctt cgagttgtcg
     9601 gaagctcagc ttgaatatgc agcgcgtgat gccgcggtcc ttttgccgtt gcgtgaaaag
     9661 ctgatcgatc gtttgaagtc ggagtcgctc gtgagagtcg cgcaactcga gtttgagtgt
     9721 gtgatgccgg tggtcgatat cgagctggcg ggcttttaca tgcacaagga gcgctggctc
     9781 gagcagctaa gcatcgtcga aaagcgaaga gtcgagcttg ccgagcagtt gcagcaagtg
     9841 ttagcggaag agtcgtcaca gggatctctt ttcggtggtc cacaacgtga agacatcaac
     9901 ctcgattcgc agcagcagtt gactaaggcg ttaactcgtc tcggcattcc ggttcccgat
     9961 tcgacgcgca actggaagct ccagccgctg gccgttgaat atccgattat cgggacgttg
    10021 cttgaatatc gcaccgtgca gaaggcgctt acgagctacg gtgaaaacat gatcgggatg
    10081 atcaatcccg tgacgaaccg tctgcacgcg gatttccggc agattggcgc gccgacgggt
    10141 cgcttcgctt gtacgaaccc gaatattcag caggtgccgc attcggtcga ataccggcgt
    10201 tgtttcagtg gttatccgga tgggcgaaag ctgatcattg cagactattc gcagatcgag
    10261 ttgcggattc tcgcggagtt ttccggcgat cgcggtttca tcgaggcttt caactccggc
    10321 gccgatttgc atcgcgtgac ggcggcacag gtcttcaacg cgtcgctcga tcaggtgacg
    10381 aaagagcagc gtgattttgc gaagcgtctg aactttggtg tggtctacgg catcggcgcg
    10441 cagcgctttg cgttgatgac cggactgacc gtgcccgatg cggagaatgt tttgcgccgc
    10501 tactttgcga cgtatcgtca gctcgataca tacttgcgcg acgcggcgaa ccgggcagtg
    10561 agcgagcgac aggcgcggac tgcttccgga cgtctcgttc gtttccgtta cgacgagaac
    10621 gaccgccaac agatctcgat gacgcagcgc aacggcaaga acaccccgat ccagggaaca
    10681 agcgccgaca ttctcaaacg cgctctaaaa ctgctgcgag atgaattgcg cgaaacaaac
    10741 gcgaaggtgg tcaacatcat tcacgacgag atcgtcgtcg aatccgacgc cgacgaggcg
    10801 caggacattg ccgaaaaagt cgagcgcgta atgcgcgtcg ccggtgagga atatctaaaa
    10861 accgtccccg taaaagtcga aacagaaatc gcagacgaat ggcttaaata acccgatccg
    10921 cgttttcaat ccgcgttcat ctgcgtctcc aaataaagta gataggtata cctgtgaaca
    10981 ccagcaagta tccaatcccc gacgtcgacg gcgccagtat cgccaaatca ataatgaaca
    11041 aacccgcaat cacgatataa agcaacggca cgaacggata cccgaccgtc cggtacggcc
    11101 gctcaagctc cggcgccttg cgtcgcatca cgatcacggc cccaaccatc aacacataaa
    11161 agatcagatc cgctgagaca atgtattcga gcagttgcgt gtaaacattc ccgaacgtgt
    11221 acgtgcctgc ctgttgatta accgtcaccg tccgcggcaa cacgaggaac gacgcccaca
    11281 atccctgcgc gatcaacgcc accgccggca cgtgaaactc attcacgcta tcaacccgtt
    11341 tgaagaacaa gccatcacgc gccatcgcgt aataaacacg cgcgcccgcg agtatcagcc
    11401 cgttattaca accaaacgtc gagaccatga tcgcgagcgc catgatcacc gtcccgcgtg
    11461 gtccaaacac cgcctgcatc acggccgttg cgacgcgacc ctgcggcgca ttttgaattc
    11521 ccgcgagcgg cagtgtcacg acgtaagcaa gattcgcgag caggtagaac gtcaccacga
    11581 gcccgcatcc aagcaacaat gcgcgcggca aatttttccc cggatcacgc acttcacccg
    11641 ccgtgaacgt cacgttgttc cacgcagact gcgcgaatag tggtccaacc atcgcccggc
    11701 ccagcatcat cagcagtgcc aacaccagca aaccttcacc cacaggctcg aaccacacct
    11761 gcgacgccga aggtttccag ccgttctccc acggcgccca ccacgaagac gtgaacgccg
    11821 cgctgccatg attccagcca acaatcaaac caatcaaaat cagtccgagc aacgcggccg
    11881 ttttcgtaaa cgtgaaagtg ttctgaatga tctttccggt ttgcagaccg cgcgtgttga
    11941 taaacgtgag aaagagaatc atcacgaccg caagcaattg ctggctcgac aaactcagcg
    12001 cgtaaccatt gaacgagagt tgattgatcg tgaaaggctt caacagcacg atcggcgcga
    12061 tcaggtatcg atctccggca acgcgatcgg tgagtacgcc ggtgaagttt gcaaacgcga
    12121 ccgcgaccgc cgcgatcgtc cccgtctgaa tgatcagaaa cagtccccag ccaaacagaa
    12181 acccgatcga tggcccataa gcttcgcgca ggaaaacgta ctgtccgcca gcgcgtggcc
    12241 acattgccgc gagctcggcg caacagagcg cgccagtgat cgtgagcacg cccgcgagcg
    12301 cccacgccac gagcagccag cccggcgagc cgacgagccg cgacgattca gcggaagtga
    12361 tgaagattcc cgatccgatc atcgagccta cgacgatcat cgtcgcgtca agcaggctca
    12421 gaccacggac gagttttggg gcggattcgg tggccatcag gtccgacgta tagtgccaca
    12481 gataggcgcg gatgaatacg gattcctatc tatgttatct tcccgtttcg gagttttcgc
    12541 cgccatatga aaaaagacct aatcgctctc ataactaaca aacaagcgca catcggcgtc
    12601 atcggcctcg gctacgtcgg cctgccgctg ctcgtcgagt ttacttccag gggatttcac
    12661 ggcaccggtt ttgaagtcga cgaggccaag gcgcaacaga tcaacgccgg cacttcttac
    12721 atcggcgacg tgtcgtcgcg caaagtgaag gacgtggtcg acgcgaagcg actgcgagcc
    12781 accacggatt tcagtcatct gagcgagtgc gatgccatca tcatctgcgt gccgacgccg
    12841 ctgcgcaaaa ccaaagagcc ggacgtctcc tacattctcg ctgccgccga acagatcgcg
    12901 gagcgccttc gtcgcggcca tctgatcatt ctcgaatcga ccacttatcc cggcacgact
    12961 gacgaagtgt tgttgccgat gttcgaagcg aagggactga agctcgacga agatttcctg
    13021 cttgcgttct cgcctgagcg tgtcgatccg ggcaatccgc agtttcagac gcacaatata
    13081 ccgaaggtcg tcggtggtgt gaccgacgat tcgaccgtcg tggcggcgca tctttattcg
    13141 cagatcgtca atgacgtgca tgcagttagc tcggcgcgtg tggccgaagc ggcgaagctt
    13201 ctggaaaata cgtttcgagc ggtcaacatc ggcatggcaa acgagatggc gcgactgtgt
    13261 tacaccctca acatcgatac gtgggaagtg attcgcgcgg cagcgaccaa gccgttcggc
    13321 ttcatgcctt tttatcccgg ccccggtatt ggcggtcact gcattccgct cgatccgcac
    13381 tatctctcgt ggaaggcgcg ccagcacggt ttcgattcgc gcttcatcag tctcgctgag
    13441 gaagtaaatt cgcgcatgcc cgagcacgtg gtccaacttg ttgcggatgg actcaacgac
    13501 gcacgcaagg cgatgaacgg ctcgaacgtg ttgctgctcg gcgtcgcgta taaaaaagac
    13561 atcaacgacg tgcgcgagag tcccgcgttg tcgatcatcg atcggttgcg tgcgaaaggc
    13621 gcaaacgtgc gttatcacga tccgtttgta gcagagttac gctttgacga cgcgcacacg
    13681 gattccagcg gcgagccttt aagctctgtg tcgctgacaa acgatgagct gcaatcggct
    13741 gactgtgtga tcatcgtcac cgatcacagc gacatcgact acaagcgcat ctgctccatc
    13801 gtgccgctca tcgtcgacac acgcaacgcg ctgaacggtg atattaggcg cgaaagcagc
    13861 gcacggatca ttcgtctgta atttgggcca caaaaggcac aaaaaggacc acaaaaggca
    13921 caaggatttt tctgagtggt tagaatggca acttcttaat aggtttttct ttttgtgctt
    13981 tttgtgcctt ttgtggctaa aagaaaatgt tgaaagtcat taacgtcgtc ggtgcgcggc
    14041 ccaacttcat gaaagtggcg ccgatcgtgg ccgcgatgaa aaaacgcccc gacaccttcc
    14101 tgccgctcgt cgttcacacc ggacaacatt acgacgcagc gatgtccgat tcgttctttc
    14161 gcgatctcga tctgccgcaa cccgacactc atctgggcgt cggctccggc tcacacgccg
    14221 cgcaaacggc tgcggtgatg gagcgcttcg agccggtcgt gcttcgtgaa aaacctgact
    14281 gggtgctggt cgtcggtgac gtgaactcga cgatcgcgtg tgcgctcgtg tgtgtgaagc
    14341 tcggaataaa agtggcccac gtcgaagccg gactgcgcag tcgcgatcgc acgatgccgg
    14401 aggagatcaa tcgcctgttg accgatcaga tcgccgattt actgttcaca ccatcgcacg
    14461 acgctgatga aaatctgatc gctgaaggca ttcccaagga gaggatctgt ttcgtgggca
    14521 acgttatgat cgactcgctc caacgaaacc tcgccgtcgc gcgcgccttg ccgacacacg
    14581 aacaattagg catcgacgga gattacgctt tactcacgct gcatcgtccg tcgaatgtcg
    14641 atgtgcgcga ctcattcgac cgcatactca cggcgctcga agtgatcacg gagaagttgc
    14701 cgatcgtttt tccagtacac ccgcgcacaa gaaaaacaat caccgagctc ggattgagcg
    14761 agcgcgtgga cgcgataaaa gatttgcgaa taactgagcc gctcggttat ctcgacttcc
    14821 tcaacctgtc gagcagcgcg tgtctcgtgc tcaccgactc cggcggcatt caggaagaaa
    14881 cgactgccct cggcattccg tgtctcacgc tgcgcgagaa caccgagcgt ccgatcacgg
    14941 ttgagatggg aacaaacgtc atcgtcggca ctgacacgac gaagatcatc gccgccgcaa
    15001 aagccacgct aaacggttcc gcaaagaagc ctgtcgtacg gccgccactc tgggacggtc
    15061 acacgtccga gcgcattctc gacgcgctcg aacaatctcg ctgatgccag ttctgaataa
    15121 aatcaagcgt gcgcttcggg gcgaagtaaa actcacgacc gcagcgcgcg aagccttgcg
    15181 acgcggccgg gtttcgcttc aacagcggaa agaacgcgcc catctcggcg agagtgaacc
    15241 gctcgccctg agacaacctt tcgcgcgcat gagtgctgat gagttatcag gatatttccg
    15301 gggaccacgc caggcgaagt ttttcgatca ctcctggccc ttgctcgatt ttgacgaaca
    15361 ggctttaggg aaagagattc agtggcgacg cgatccgctg tcaaattatg tttggccgct
    15421 tgattaccac cgcgacatca agttgatgcg cacggatggc tcagatattc gtgtgctttg
    15481 ggaattgaac cggctcggac attttctccc gctcgcatgc gcttacgcgt caacgcacga
    15541 tgaaagatac agcgccgagt ttgttaccca actaaaaagc tggcacgaac aaaatcctta
    15601 cgggcgtggt ccaaactgga cttgtgccat ggaagtggcg ttgcgcgcga tgaatctgct
    15661 ggcggctttc gaagtcttcc gtcagtcgcc gcaaatgccc gtggagttct tcctgcaact
    15721 actccagcaa cacggtaact acatccgacg caacctcgaa ttctcttatc tcgcaacgag
    15781 caatcattat ctttcagacg tggtcggact actctggctg ggtttattgc tgccggagct
    15841 tcgcgacgcg aacgcgtggc gcgactttgg cctggaccaa atgctgcgcg agatggacaa
    15901 gcaggtgctg gctgatggcg cggatttcga agcgtcgact ggctatcacc gcttcgttac
    15961 cgagctcttt ctttactcgt tcttactttg tcgtaccaac aacgtcgaga ttccaaagcg
    16021 atactggtcc aaactcgagc aaatgctgct ctacattcgt gcttacctgc ggcccgatgg
    16081 gtttgcgcct ctgattggtg atactgacgg cgggcaggtt ttaccgtttg tgcgccggcg
    16141 cgccgatgat cacgcttatc tcctggatat tttgaaactc gagactgagc acgtcggttc
    16201 tcgagcattt ccacacgcgg gcacgtacat catgcgcgat ggcgatctgt atttatgttt
    16261 caacgcgagt ggtgaaggga tcaatggtcg tggatcgcat ggtcataacg atgtgttgag
    16321 tatcgaggtc tcggctggtg gtcgcgcgat catcgtcgat ccgggaacat acgtctattc
    16381 tgcggacctc gcgaagcggc acgagtttcg atcgaccgcg tatcattcga cagtgcagat
    16441 cgatggactc gaacagaaca cgatccgcgt cgatacgccg tttgtgatcg ggaacgaagc
    16501 gaagccgcgt gtgctggagt ggaaaacgag tgatgatttt gacaaggtcg tcggcgagca
    16561 ttatggatac cccgtgacgc atcgtcgcac cgttacgttc gacaagcgcg cgcgtctgtg
    16621 ggttatcgat gatgagttta tcggcgatgg tgaacacgtt tacgaggcga gatttcattt
    16681 cgcgcccggg ttggatgtgc gtgtgaatgg cccggcagtt gaagccggta gtttagttgt
    16741 gactttgttg ggttccgata ttcaaccggt attgcaaacc caatccgcgt cgatcgatta
    16801 cggccagatg agtgattcta tatccgcttg ctggaaaatc tcgggaaaac cacgcaagct
    16861 tacctggcaa ataaaatccg tgtaatccgt ggctaaagaa taatgtgtgg catctgcgga
    16921 gtttgggaat acggcgcaag cgatggttcg attgaatcct cgctgctggc gcgcatgcgc
    16981 gatgagatga ctcatcgtgg gcccgacgac gccggcgatc tgatctttga caatcatcgc
    17041 ggcggcttcg gttttcgtcg tctctcgatc atcgatctct ccgccgccgg ccaccagccg
    17101 atgcacggct gcgacaaacg cacgtggctc gttttcaacg gtgagatcta caaccacgca
    17161 ctcatccgcg ccgatctcga aagacgcggc cacaagtatt catcgcgtac cgactccgag
    17221 acgattctgc atctctacga agaacgcggt ctcgatttca tccacgagat cgaaggcgac
    17281 tacggcatcg cgatctggga cgcaaacaaa gaacgtctcg tgctcgtgcg cgaccgtgtc
    17341 ggagtcaagc cgctttactt ctatcacaaa gacggccgct tcatctttgc gtctgagatc
    17401 aaggcgatcc tgcaacatcc cgcggtcacg cccgacatcg acgagcagtc gctttatcac
    17461 tatctgactt tcctcacgac gcccgcaccg gcgacgctct ttcgcgacat acataagctg
    17521 cccgccggtc acgttttgac attgacgcgc gacggcacgc tcaacattca gcaatactgg
    17581 gacgcgcttc ctccaaaaca aaagatcgcc ggcactgaag aagagcaacg cgccgagata
    17641 ctgcgcctgc tgcgcgagtc gattcgaaag cgaatgatgt cagacgtgcc gttcggtgtg
    17701 tttctgtcgg gcggcgtcga ttcgtctgcg aatgtcgcgc tgatgtcgga acagatgtcg
    17761 cgtccggttg agacgtttac agtcggtttc agcgatgccg agtatctcaa cgagctcgag
    17821 cccgcgcgcc gcatcgcgaa acagtttaaa accaaccatc acgaggtgat cgtctccgag
    17881 aaagagatgc acgactttct gccgagcatg gtctttcacc aggacgagcc gattgccgat
    17941 ccggtttgcg tgccgcttta ctacgtttca aagctcgcgc gcgactcggg aacgatcgtg
    18001 gtccaggttg gtgaaggctc ggatgagatc ttcagtggtt atgagaacta tgtgcgtcac
    18061 ctgcgcattt acgaaaactt ctggcggcat gcggaggtgt tgccgctctc gttgcgacgc
    18121 gcgatctcga atctttcgcg tccggcgctc gaagcgactg gtcgaaagcg tgcggcgatc
    18181 gagttgatac ggcggttggg tgcggatgag cctttgttct ggggcggcgt ggtggtttac
    18241 gacgagacgt tcaagccgcg cgtgctgtcg cagtcggtgc gcgagcgttt gaatggtctt
    18301 tcatcgctgc gggtaattga gaagtacttg aagcatatcg agcaggagcg gccgaacgcg
    18361 gattttctcg cgcgcatgac gtatctcgag ttgaagttgc gactgccgga gttgttgttg
    18421 atgcgtgttg ataagatcac gatggcgacg agtgtggagg cgcgtgtgcc gtttctcgat
    18481 catcatttgg tggagtatgc gctcgggctc gcgcgtacgt tgaaggtcga agggacgagt
    18541 ggaaaacata ttttgaagcg atcgctcgag gagatcctgc cgcgtgatct gttgtatgag
    18601 cggaagcgtg ggtttggcgc gccagtgcgt gagtggttcc gtgaaggatt gggtggctgg
    18661 ttcgacgacc acttgattaa ctcgtcaatg cggcgtcgag atttgctgga ttacgggttt
    18721 gtgagccaga tgttggaaga gcatcgtgcg aagacgcgcg actggggatt tcatctctgg
    18781 gcgttgctga atttgagctt gtggtatgag agatggatcg attgaaccca tccacagatt
    18841 acacagattt agtctgcgta atctgagtaa tctgtggata catattggct ggacgttcgc
    18901 gacacgcctg ttgatgatct ttaacagcgt cgtggcgggg atcatcgttg cctggtggct
    18961 gggcgcggag ggtgtgggcc agctcgcggt gatcaatgtg gccgtcgcga ctgtggttca
    19021 gattggcagc tttggactgc catcatcaaa cacgtatttc atcgcccagg atcaatcgca
    19081 cttccgtgct gccgccatca actcattgat cttcgcgctc ggtatcggtg cggtgctcgc
    19141 cttcgcgctc acagccgttg cctcaatcaa gcccgattgg tttggattcg tttcaccgca
    19201 actggtccgt atagcagccg tctcgatccc atttcaacta atcacactaa ttggtctgaa
    19261 tatcctgctc gcagtgggaa aaattcgcga gttcaacctg ctcgacctcg ccggtcaatc
    19321 attcgtactc atcaacgcgg ccctggtctt gttactcctc aagcgcggcc tcgaaatgct
    19381 agtcacgttc aacacgctcg ccagcattct cgtcgccagt gtgattacgt tactgcttgt
    19441 gatctcagca aagtcgctca cccaatcgaa atggcgcgcc gacatcgctc tcctgcgacg
    19501 catgatcgtt tacggtttga agtttcacat ttccatcctc gcgggcgcga tcatcattcg
    19561 cgcagatctg ctggtcgtaa atcattttcg cggcgcagcc gaagcgggcg tctacagcgt
    19621 cgcttcacag tttgcgttgt tactgatgtt gctacccggc gtgatcgcga cgttgctgtt
    19681 cccacgcgtg actagcgaac aggacgtgcg cggtgaaacg acgtgcctcg tgagtcgcta
    19741 cactacgtta gtcatgttcg cctgttgcct cgccgcagtg ccgtttagtt tcctgttgcc
    19801 ttttatctac ggtccggctt tcgctgatgc gacgaagctg cttctgatcc ttttgccggg
    19861 cgtttacctg atcggactgg aatcagtgct ggtccaacac ttcaacgcgc tcggtctgcc
    19921 gcgtgcgatc ccgatttact ggctcgtcac attgatactc aacctcgtgc tcgtattcac
    19981 gcttgtgccg cgctacggtg cacaaggcgc ggcgatcgcc tcatcgatca gctacgcgat
    20041 gatcttcgcg cttgtcgcac ttcactttca caccagcacc ggacgatcgt ttgccgaagt
    20101 gtttgtgctc gggagcctgc gcggatgaga gtcgtgctcc tcgccctcag cggcgacctc
    20161 gaccgctggc gagacaaact gctcgcgctc tatcccgaca gctccatcga tctgatctca
    20221 cgcgccgaat tcgaaaaggg cagccacatc gcgcgcctga gatccctgcg cgcgctcagc
    20281 ccggacgtgc tcgcgatcgc gaccgagcgt ttaccctggc aacgtggtca gaaccttttc
    20341 atggtcttcg gcgcgctcgc gggcgcgcgc gaagtgctga tgctcgacgc gcacggcgaa
    20401 atgattcgca agtcgcgctc gaaagttttg cttagtgcgc ccgcacgact cagccgcgag
    20461 accatcgcga gcgccggcga catcgcccgc tcgcgtcgtg aattgcagca gctcgaagaa
    20521 acaacattgc ggttcgtaga catgccgcgc aagatcacgc cgcgatcgcg cgtgcgagtt
    20581 gtttacctgc gctcgacgcc tggaccaggc acacaggcgg gcggggctgc gagtcacatc
    20641 aaaggcgtgg ttgaaggcct cgaagcgctg ggcgctgagg tgcagatcat cagcaacgat
    20701 ctcatcgccg gcatgaacgc cgcggacgat cgctttaccg tgatcccgcc acaacccggc
    20761 ggcggcacgc gcgcgctttt cgacattcac aacaacctgg tcttcacgcg cggcgcggtg
    20821 ccgttgatcg agcaggccga tcccgatttt atttatcaac gctacgcgcg gtttagctgg
    20881 gccggcgtgg tggctgcgaa tcgcacgaag cggccgttgt ttctcgaata caacggttcg
    20941 gaagtctggg tgggccgtta ctgggatcga gtggggcgtc tcgaattgct ggagcggtac
    21001 gagcgattga accttgacgc tgcggcgcgt atttttgtcg tctccgatgt tgagcgccgg
    21061 aacctcgagg cgcgtggtgt cgcggcggag aagattgttg tcaatccgaa tggcgttgat
    21121 gtcgagcggt ttcggccggg tgttggtggt gcgggggcgc gacgcgagct tggcattcga
    21181 gatgatgagg tggtggcggg ttttgtcggg acgtttggac cgtggcatgg cgttgagaag
    21241 cttgccgagg cgatcaagac gattccggca aacgagaagg tccggtttct tttcgttgga
    21301 agcgggagct tgcatgcaga ggtc
//
LOCUS       DASSFQ010000275        12314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_568566, whole genome shotgun sequence.
ACCESSION   DASSFQ010000275 DASSFQ010000000
VERSION     DASSFQ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12314
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568566"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..2615)
                     /locus_tag="VFH96_09950"
     CDS             complement(93..2615)
                     /locus_tag="VFH96_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974336.1"
                     /translation="MRLAHRALIAALVSLVLCAAVSAQTLRSPNDPRNQSPAVGTGGP
                     EGGPTGLFTIYDGSTLRRGEFTFSIAYSNYDRDPGNVDIVDVPLSFNVGINDHIEVFF
                     KTNGYRGIKVNNPQNLSSFYLPNSQLFCGAALCSPAAIVLAPSGPNVGTLANTAVFRP
                     LNNQPFVQFPFTGGSAGTFGLAPGQQQFGFPGFNAQLGPPVVQPNSGNFGPADNFPGV
                     GSPVGGILPGVVLATTTLPATALTRPITVPLTFTVAPSYLPDAPFVNRLYGESNFNNM
                     VFGAKIRFTGPNNPLGVALIPFYRWWLDKADDLSGFNQMQRGAGPGGDIGDFGLIGVV
                     DGRLGKHVNVSANGGYILNSNPKGAGGDFVLLDRPDELLAGVGFDFPINEHFQPVAEV
                     RSSTYVGGHTPNAFNNNPVEVLGGIKIYPRRWFGIGLAYRRHLNQQDMDHFNPTDFNI
                     PIQQVTNVNVIGRGLVVVPGTSRPVTSQGFPIGFLFSEEPNGFILQFWAGRRNARIPP
                     KPPNVAPVVSGVTPSISSVLRPCPEGQSSATCTPTGSEVTLVANATDQDNDQLLYTWS
                     VTGGRLSGEGRQVTWDLSGVANGSYTATVEVNDGNQHTANGNTTVTVADCTGCVPPPP
                     PCPTVSVSCPTDVEPNAPITYTASVSGGGAGATWTYNWSVSAGTISSGQGTSTITVDT
                     NGQSGSSITATVSIGGADPSCTGTTASCTTSVKVAPPPATKFDEYGNIRFNDEKARLD
                     NYAIQLQNNPGSQGTIIVYGSCAGEAQQRGDRAKDYLVNTRGIEAGRITVVDGGCRAD
                     LTVQLWVVPQGAAAPTVDTTGAIEPCPECKRGKAPRRRRGDE"
     gene            complement(3034..4326)
                     /locus_tag="VFH96_09955"
     CDS             complement(3034..4326)
                     /locus_tag="VFH96_09955"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET6974337.1"
                     /translation="MVSMYSTTYVDDIREIKEENPFESMMSRFDRAAQLLDLDPDLYA
                     VLRVPNRELKVYIPTKMDSGRIQVFEGFRVQHNFARGPAKGGIRYAPDVNIDEVRALA
                     AWMTWKCAVVNVPFGGAKGGIICDPQQMSMGELERMTRRYAAELLDFIGPEKDVPAPD
                     MNTNEQTMAWIMDTYSMHARHTVNAVVTGKPIDLGGSAGRREATGRGILIVVNEAIKR
                     FKMRPVETRVVVQGSGNVGGIGAQLLYETGYKVVAISDIHGGIYNPNGIDIPKALTYL
                     KNTRSFEGYEGVEFVNNSELLELECDVLVPAATENQINSQNADRLKCKVLAEGANGPT
                     TAAADEILHQKGIFVVPDILANAGGVTVSYFEWVQDRMGYFWREDVVNERLQDKMVAS
                     FNDLCRYADAHNVDTRTAAYMLAIDRVAYDTRMRGIYA"
     gene            complement(4385..5221)
                     /gene="larE"
                     /locus_tag="VFH96_09960"
     CDS             complement(4385..5221)
                     /gene="larE"
                     /locus_tag="VFH96_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET6974338.1"
                     /translation="MNLDTKEERLREIFRELDSVIVAYSGGVDSSYVAYVANAELGPR
                     AVCITGQSASLPEYQRAEIDSVVQKFGFQHEVIQTEELENPGYRANNPDRCFFCKDEL
                     YTKLELLARTRGIDNIVDGSTVDDLGDYRPGRRAASQHAVRSPLIEAGLTKSEVRELS
                     RRATLPTWDKPASPCLSSRIAYGTTVTIERLSKVDRGEEILREFGFREFRVRHHDQLV
                     RIEIAPAEMDRVLRKELVDELARRFRQLGFKYVTLDLDGFRSGSMNEVLDRPQITQIT
                     QS"
     gene            5383..6822
                     /locus_tag="VFH96_09965"
     CDS             5383..6822
                     /locus_tag="VFH96_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974339.1"
                     /translation="MSKKLLLVATCIALVSVCLKGSAQTVEAFGDDAADPVRLFERGQ
                     SAHARGEIEKALAYYEQALKVRPEFPEAEFQRGNALASLGRLAEADAAFRRAITYKKN
                     WSLPYSALGVVLVRQNHDKEAEQFFNQALTVDNKDNVALRMLADLRLRAGDTKSALDF
                     AKRATANPDAPASAWVALALAEKAVGNTAGAKAALEHVLANEPENVAALIERADLFTN
                     EKNFEPAIADLKTAAKLKPDDKAILSRLAYVLQQAGKTEEAQAAAKSAGIETQPPSNR
                     NSDIGVVGTPEEIEAANSTDPAIARKSLEKLLEKNPRSAMLLGRLGASYRTDDPARSL
                     DYYRRASEIQPDAPEYALGYAAALVQARRFPEAAGILRQVVKMSPDNYVAHANLATAL
                     YEAKRYQEAIPEYEWIVSAKPEISVVHYFIATSHDYLGEYPEALLSYEKFLTVADPKT
                     NQLEIDKVKLRLPTLRRQIQLGEGAKKKP"
     gene            6833..7432
                     /locus_tag="VFH96_09970"
     CDS             6833..7432
                     /locus_tag="VFH96_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974340.1"
                     /translation="MTRETLRRIIFPALLVLACVPAVFAQEEQPKPSPQPTPLTAPPP
                     FKMIVKEERAEMDAATKDAQKRLKLTMEFAGGHLTRAEQHTARGDFEAASAEVGMYHA
                     LIENALEFLSSFKRDSNKTRDLYKRLEMALRADGPRLTAMRRITPLEFAVWIKQVEDF
                     ARDGRTEALNSFYGQTVVRDPQKVEKPSPTPTPKSYKQP"
     gene            7429..7977
                     /locus_tag="VFH96_09975"
     CDS             7429..7977
                     /locus_tag="VFH96_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974341.1"
                     /translation="MITRTLLVSSLVLCFAIVSYAATPQRDHLTPQEVDLVKEAQALD
                     KRIDVFIKAAERRLIVINNSAAANAKVLKKDSERWGELPTGSRAELVGDIARIFDEAI
                     TNIDDVSARDERNPLISKSLRKLATAVNSILGQLKPLSADAKSDAEIASFELLHEDAQ
                     SILEAANKLPPEVVDKKAKKNN"
     gene            complement(7988..10120)
                     /locus_tag="VFH96_09980"
     CDS             complement(7988..10120)
                     /locus_tag="VFH96_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET6974342.1"
                     /translation="MKTILVLFLLVVITLTTPVTFAQTAIDGFSAERSTAERRWEEQF
                     RAVPDPKSAREHLRRLTVEPHIAGTKEDYNTAVYVRDQLRSFGLSADLKEYEVWLNYP
                     NAPTVLELITTRRQPLSVKEAPVPGDPSSSHPKISPLFNGFSASGDVTGPVVYANYGL
                     PNDYEDLKKAGVDVKGKIVIVRYGNSFRGVKAKVAEQNGAIGCIIYSDPEDDGYMQGD
                     VFPKGPWRPVASGQRGSVQYLFDYPGDPLTPGKPAIAGTPRLKPEEATDLTRIPVQPI
                     AYDVARTILSQLKGPLRPRGFQGGLPFAYHVGGTNDVKLRLKTDMDYKLRTIWNVVAR
                     IDGNEEKDRWVVLGNHRDAWVFGAVDPNSGTSAMLEVGHGFGELLKQGWKPRRTIFLC
                     SWDAEEYGLIGSTEWAEEMADELRQKAVAYLNLDAAVSGPHFGASSVPSLWKLMRGAT
                     RDVKDPKTGKSVYQQWQDRSRENRPEGDQDMHEARIAALGSGSDYTPFLQHLGVASTD
                     MGFNGDYGVYHSAYDSFYWMDHFGDPGFTYHVAAAQLWGTLAMRLADADGLPFDYTDY
                     AHQIRDFFTEAMRLARIRNLASSIDDKAMTSAIDDFAKEAERVEKDRQEAVRKGDRAE
                     LAKINDALVQAERQFIDARGLRGRAWYKHQIYAPGFYTGYAAQPLTDFRQAIDDRNSG
                     NAKESLERIVDSIKRVTRTLKSAADKTD"
     gene            10143..10709
                     /locus_tag="VFH96_09985"
     CDS             10143..10709
                     /locus_tag="VFH96_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6974343.1"
                     /translation="MDSRYYPGVEADVPQLSTELRNVFDSEFEVQTFQVSNTSIFQAR
                     KSSTLRDLTGLSSALTIKITPEPGGTRVEIGMQKWFDKAAVAAVGVVVFPPLLALAAL
                     GAYWQYKLTEDAWKIIENHISRKAGGYVPPLPGRCGTCGGANIAESDFCSSCGANLRV
                     GRTCSNCGAPQKDNAARFCNRCGKPLSN"
     gene            complement(10797..12257)
                     /locus_tag="VFH96_09990"
     CDS             complement(10797..12257)
                     /locus_tag="VFH96_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6974344.1"
                     /translation="MSFLDILHLALRNLRQAKLRAILTTMGVIVGVAVIVTMMSFGLG
                     LQRNMLDRFKALDFFNEIQVFGQGLSTLAGLDRSSRGPEGEPRNDRRSDRSPTRILDD
                     KGIKEIAAIPGVAYVEPSVSMNVFVRSNGKLRSTNIGGANIPNAATRFQHFAAGKMIS
                     SPTAGEAVVSERFIREFGYEKPADAIGKTLELLAPPSENKSENKKEEEEEDTPNFFGI
                     PLDDPGLDESNPDVEIQTFRIAGVLGEVKEGVGQGGLRGLFPGAQIYIPLQLAHDWAI
                     RHRSPMGQVALALARRGGNLSEGQNEGYEAAVVRVSDPKHLTEVRQKITELGFGSFSI
                     VDEIEQIRTVFLIIDSVLGLLGGISLLVASFGIANTMIMSILERTREIGIMKAIGAED
                     REIKMIFFVEAAVIGTVGGVVGVLIAWGIDGIANRIAYRFILKPQGASFIDFFSLPIY
                     LSVGAILFALFVSILAALYPASRAARIDPVRALRHD"
BASE COUNT         2768 a         3603 c         3324 g         2619 t
ORIGIN      
        1 tttgtgcctt tttgtggcta aaaagaaatc gggcgatgcc gtgaagcatc gcccgaagtg
       61 gttaaggatt tggctaagcc aaattccggt gattattcat ctcccctgcg ccggcgcgga
      121 gcttttcctc tcttgcattc cggacaaggc tcgatcgcgc ccgtggtgtc aaccgtcggt
      181 gcggctgcgc cttgcggcac gacccagagt tgaaccgtca ggtctgcacg acatccaccg
      241 tcaacgaccg tgatgcgtcc agcttcgatt ccgcgcgtat tgacgagata gtccttagcg
      301 cggtcgccac gctgttgcgc ttcaccagca cagctaccgt agacgatgat cgtcccctgc
      361 gatccaggat tgttctggag ctgaatggcg tagttgtcga gtctggcctt ctcatcgttg
      421 aagcggatgt tgccatactc gtcaaacttg gttgccggcg gcggcgccac cttcacactg
      481 gtcgtgcaag aggcggtcgt gccggtgcag ctcggatccg cgccgccaat gctgacggtt
      541 gcggtaatgc tcgagccact ctgaccattg gtgtcaactg tgattgtcga cgttccctga
      601 ccactcgaga tggttccggc agagacggac cagttataag tccaggtcgc tcccgcacct
      661 ccacccgaga cgctagccgt gtatgtgatc ggcgcgttcg gctcaacgtc agtcggacag
      721 ctcaccgata cggtcgggca tggcggcggc ggcggtacgc agccggtgca gtctgcaacc
      781 gtcacggtag tattaccgtt ggcggtgtgc tgattgccgt cattgacttc gacagtcgcg
      841 gtatagctgc cattggcgac gccactgaga tcccaggtta cctgtcgacc ttcaccactc
      901 aggcggcccc cagttacgga ccaggtgtat agcaactggt cgttatcctg gtcagtcgca
      961 ttcgccacca gtgttacttc gcttccagtc ggtgtgcacg tcgctgaaga ctgtccttcc
     1021 ggacacggac ggagcaccga agagatcgac ggcgtaacgc cgctgactac tggcgccaca
     1081 tttggtggtt tgggcggaat acgcgcgttg cgacgtccgg cccagaactg aaggatgaat
     1141 ccgttcggct cttccgagaa gaggaatccg atcgggaaac cctgcgatgt caccggacgc
     1201 gatgtgcccg gaacgacaac cagtcctctt ccaatcacgt taacgtttgt gacttgttga
     1261 atcggaatgt tgaagtcagt cgggttgaag tggtccatat cctgttggtt caggtgacgg
     1321 cgataagcga ggccgatacc gaaccagcga cgcggataaa tcttgatacc gccaagcact
     1381 tcgacaggat tgttattgaa cgcattcggc gtgtgaccgc caacgtaggt cgacgatcta
     1441 acttcagcga ccggctggaa gtgctcgttg atcgggaagt cgaaaccgac acccgcgagg
     1501 agctcatccg gacgatccaa caacacgaag tcgccgccgg cgcccttcgg attgctgttc
     1561 aggatatagc cgccgttggc tgatacgttc acgtgcttgc cgagacgacc atcgacgaca
     1621 ccaatcaaac cgaagtcacc gatatcgcca cccggaccag caccgcgctg catctggttg
     1681 aagccggaca aatcgtcggc cttatccagc caccagcgat agaacgggat gagcgcaaca
     1741 cccagcggat tgttcgggcc ggtgaaacga atcttggcgc caaacaccat gttgttgaag
     1801 ttgctctcgc cgtacaggcg gttcacaaac ggcgcatccg gcagatagct cggcgcgact
     1861 gtgaacgtaa gcggcacggt gattggacgg gtcagagccg tggcgggcaa cgttgtggtt
     1921 gccagcacga cgcccggcag gataccacct accggagaac caacccccgg gaagttgtca
     1981 gctggaccaa agttgccgct gtttggctgg accactggcg gtccaagctg cgcgttgaag
     2041 cccgggaaac cgaactgctg ctgtcccggc gcgagcccaa acgttccagc ggatccgccg
     2101 gtgaacggaa actgcacgaa cggctgatta ttgagtgggc gaaacaccgc ggtatttgcg
     2161 agcgttccga cgtttggtcc agacggcgcc agcacgattg ctgccggact gcacaacgct
     2221 gcgccacaaa acagttgtga gttcggcaga tagaagctgg aaagattttg cggattattg
     2281 accttgatcc cgcgatagcc gtttgtcttg aagaagactt cgatgtggtc gttgatgcct
     2341 acgttgaagc tcaacggcac atccacgata tcaacgttgc cggggtcacg atcgtagttg
     2401 ctgtaagcaa tactgaacgt gaactcacca cgacgcagag ttgaaccgtc gtaaattgtg
     2461 aaaagtccgg ttggaccacc ttctggacca ccggtaccta ctgctggaga ctgattgcgc
     2521 gggtcattag gcgaccgcaa agtctgtgct gatactgcgg cacacagcac caacgaaacg
     2581 agcgcagcta taagcgccct atgtgcgagt ctcatcgcat tcctcctgcg aaatgagtaa
     2641 taagaattcg taaatagtgc cgccgtaacg gaacaagtac caacaccctt ctcccgcgga
     2701 ggcccccact acagttgtca attcggttga accgcttgga ccagcatggt ccagcggcga
     2761 cgctcggacc gtggtattaa tcgagcactt cggccttact gacagtgctc aatttccacc
     2821 aattactatt cccgagtgac tcctgaactg agttcatttc ttatagaaaa tgaacatccc
     2881 cttgtcaacg atttcgtccc ttaaaccatt gcgtaattat tgagaaatct taacgctggt
     2941 ccgaggcgta aaccatgcca gaccatactc taccttaaat gggaaatcaa gcctttttca
     3001 ctacttagcg gctctgcgtt tatgtttttt ccgtcacgca tagatgcctc tcatgcgcgt
     3061 gtcataggcc acacggtcta tcgccaacat gtacgccgcg gtcctggtat cgacgttgtg
     3121 agcatcggca taacgacaca gatcgttgaa gctcgcgacc atcttgtcct gtagcctttc
     3181 gttcaccaca tcctcacgcc agaagtatcc cattcgatcc tggacccact cgaagtacga
     3241 gaccgtcacg ccacccgcgt tcgcaaggat gtcaggcacg acgaagatcc ctttttggtg
     3301 aagaatctca tcagccgcag ccgtagtcgg tccattggcg ccctcagcga gcactttgca
     3361 cttcaaacgg tccgcgttct gagaattgat ctggttctca gtcgccgccg gcaccagcac
     3421 atcacactcg agctcgagca actccgaatt gttgacgaac tccacacctt cgtagccttc
     3481 gaacgaccgt gtgttcttca agtaagtcag cgccttcgga atatcaatcc cgttaggatt
     3541 gtaaatccct ccatgtatgt ccgatatcgc tacaaccttg taaccggtct cgtagagtag
     3601 ctgggcgccg attccgccca catttcccga gccttgcacg accacacggg tctcgaccgg
     3661 cctcatcttg aaacgtttga tcgcttcgtt gactacaatc agtatgccgc ggcccgtggc
     3721 ttcacgccgg cccgccgatc cgccgaggtc gattggctta cccgttacca cggcgttcac
     3781 ggtgtggcgt gcgtgcatcg agtaggtgtc cataatccac gccattgttt gctcgttggt
     3841 gttcatgtcc ggagcgggca cgtccttttc cggtccaatg aaatcgagca gctcagcggc
     3901 gtaacggcga gtcatgcgtt cgagctcgcc catggacatc tgctgcggat cgcagatgat
     3961 gccgcctttc gccccaccga aaggcacgtt gactaccgcg catttccacg tcatccacgc
     4021 ggccagggcg cgcacttcgt cgatattaac gtcaggcgca tagcgtatac cgcccttcgc
     4081 tggaccacgc gcgaagttgt gctggacgcg aaaaccttca aacacctgaa tgcgtcctga
     4141 atccattttc gtggggatgt agaccttcag ctcgcggttt ggaactctta atacggcata
     4201 gaggtccgga tcgaggtcca gtaactgcgc ggcgcgatcg aatcgcgaca tcatcgactc
     4261 gaacgggttc tcttctttta tctcgcgaat gtcatcaacg tatgtagtgc tgtacatgct
     4321 aaccattaga gcgtctccat ttttcatcca cagatggcgc agatttcaca gactgagctc
     4381 gagcttaact ctgtgtaatc tgcgtaatct gtggacgatc taaaacttca ttcatcgatc
     4441 cgcttctaaa accgtcgaga tcgagtgtga catatttgaa tccaagctgg cgaaagcgtc
     4501 gcgccagctc atcaaccagc tctttccgta acacacggtc catctctgcc ggagcgattt
     4561 cgatccgcac cagctgatcg tgatgacgca ccctgaactc tcgaaaacca aactctcgca
     4621 aaatctcttc gccgcggtcc actttgctca gtctttcgat cgtcaccgtg gtcccgtacg
     4681 cgatgcgtga cgataaacac gggctcgcgg gcttgtccca ggtcggcagg gtcgcgcgcc
     4741 ggctcaactc tcgaacttca cttttggtta gaccagcctc aatcagcggg ctacgcactg
     4801 catgctgcga cgcggctctt cttccgggac gataatcacc gagatcgtcg accgtcgagc
     4861 catcgacaat gttatcgatg ccacgcgtac gggccagcaa ttcgagtttg gtatacagct
     4921 cgtctttgca gaagaaacag cgatcgggat tattcgcgcg atagccgggg ttctccagct
     4981 cttcggtttg gatcacttca tgctgaaacc caaacttctg aaccacgcta tcgatctccg
     5041 cgcgttgata ttcaggcaga ctggctgact gtccggtgat gcaaactgca cgtggcccga
     5101 gctccgcgtt agcgacgtaa gcgacataac tgctatcgac gccgcctgag taagcgacga
     5161 tcaccgagtc gagttcacga aagatctctc gcagtctttc ttccttagtg tctaagttca
     5221 tgtggaatac cgcatgttag cttatgcatc aattactgcg caaccagcat cccaaacgtg
     5281 ttcttcatcc acagattacg cagattcaag gaaacaatct gtgtaatctg tggataaaga
     5341 atcgaatatg ctaacatcga agcctccccg gatcgttcgt caatgtctaa gaaacttcta
     5401 ctcgtcgcta cctgcattgc gctggtctcc gtgtgtctga agggttccgc gcagacggtc
     5461 gaagcgtttg gcgacgatgc ggcagatccc gtgcgacttt tcgagcgcgg acagagcgca
     5521 cacgcgcgcg gcgagatcga aaaagcgctt gcctattacg aacaggcgct caaggtgcgg
     5581 cccgaatttc cggaagcgga gtttcagcgc ggcaacgcgc tcgcctcgct cggacggctc
     5641 gcggaagcag acgcggcatt tcgtcgtgcg atcacttaca agaaaaactg gtcgcttccc
     5701 tactccgcgc tcggcgtcgt gttagtgcga caaaaccatg acaaagaagc agaacagttt
     5761 ttcaaccagg cattaaccgt cgataacaaa gacaacgtcg cgctgcgcat gctcgctgac
     5821 ctgcgtctgc gggcgggcga cacgaagtcg gcactcgact tcgccaaacg cgcgactgca
     5881 aatcccgatg caccagcatc agcctgggtc gcactcgccc tcgcggagaa agcagtcggc
     5941 aacaccgctg gcgcgaaagc agcgctcgaa cacgtgctcg caaacgaacc ggagaacgtc
     6001 gccgcactga tcgaacgtgc agatctattc acaaacgaaa agaacttcga gcccgcaatc
     6061 gcagatctga agaccgcagc gaaattaaag ccggacgata aagccatttt gtcccgtctc
     6121 gcttacgtgc tccagcaggc aggcaaaacc gaggaagcac aggcggctgc caagtccgcc
     6181 ggaatcgaaa cgcaaccacc gagcaaccgc aacagcgaca tcggcgtggt cggcacgcct
     6241 gaagagatcg aagcagcaaa cagcaccgac ccggcaatcg ctcgcaagtc gctcgaaaag
     6301 ttactcgaga aaaacccgcg cagcgcgatg ctgctcggcc gtctcggtgc gtcatatcgt
     6361 acagacgatc ccgcccgttc gctcgattat tatcgtcgtg cgtcggagat tcagcctgac
     6421 gctcctgaat acgccctggg ctacgcggcg gcactggtcc aagcgcgtcg cttcccggaa
     6481 gcggcgggaa ttctgcgcca ggtcgtcaag atgagtcctg acaattacgt cgcgcacgcg
     6541 aacctcgcga cggcgttgta cgaagcgaag cgctatcagg aggcgattcc ggagtatgag
     6601 tggatcgtat cggcaaaacc ggaaatcagc gtggtccatt attttattgc cacgtcgcac
     6661 gattatcttg gagaataccc cgaggcactc ctttcgtatg aaaaatttct gacggtagct
     6721 gatcccaaaa cgaatcagct cgagatcgat aaggtcaaac tgcgattgcc aacgctgcgg
     6781 cgacaaattc aattaggaga gggcgcgaag aagaagccct gaacggcccc gcatgactcg
     6841 cgaaacactg cgccggatca tcttccctgc actgctcgtg ctggcatgtg ttcccgcggt
     6901 tttcgcgcag gaggagcaac caaagccatc gccacaacca acgccgctga cggcgccgcc
     6961 gccgttcaag atgatcgtca aagaagagcg cgcagagatg gacgcagcga ccaaagatgc
     7021 acaaaaacgc ctcaagctca caatggaatt cgctggcgga catctcacac gcgccgagca
     7081 acacaccgcg cgcggagatt tcgaagcagc ttcggcggaa gtcggcatgt atcacgcgtt
     7141 gatcgaaaac gcgctggagt ttctcagcag tttcaagcgc gacagcaaca agacacgtga
     7201 cctttataaa cgtctggaga tggcgctgcg cgcggatggt ccacgtttga ccgcgatgcg
     7261 ccggatcacg cccctcgaat ttgctgtctg gatcaagcag gtggaagact tcgctcgcga
     7321 cgggcgaacg gaagcactca attcgttcta cgggcagaca gtggtgcgcg acccgcagaa
     7381 ggtcgagaaa ccgagcccaa ctccaactcc aaagagttac aaacaaccat gatcacccga
     7441 acactccttg tttcctcgct cgtcctgtgt ttcgcaatcg tctcttatgc ggcaactcca
     7501 cagcgagatc atctgacgcc gcaggaagtc gatctggtta aggaagcgca ggccctcgac
     7561 aagcggatcg atgtcttcat caaagccgcc gagcgacgct tgattgtgat caataattca
     7621 gccgccgcga atgcgaaggt attaaagaag gactcggagc gttggggaga attgccgacc
     7681 ggatcgcgcg ccgagctggt gggcgacatc gcgcgtattt ttgatgaggc aatcacaaac
     7741 atcgacgacg tcagcgcccg tgacgagcga aacccactga tctcgaagtc actgcgaaag
     7801 ctcgccaccg ccgtgaactc gatcctgggc cagctaaaac cactcagcgc tgacgcaaag
     7861 agcgacgccg agatcgcgag cttcgagttg ttacatgagg acgcgcaatc aatcctcgaa
     7921 gcggcgaaca aactcccacc cgaagtcgtc gacaagaaag cgaagaagaa taattaaatc
     7981 cgcgttttta atccgtctta tccgcggctg attttagtgt ccgggttacg cgcttaatag
     8041 aatctacgat ccgctcgagc gactccttcg cattgcccga attccgatcg tcaatcgcct
     8101 gccggaaatc agtcagcggc tgtgctgcat atcccgtgta gaaacccggt gcatagatct
     8161 ggtgcttgta ccacgcgcgc ccacgcaatc ctcgcgcatc gataaactgc cgctcggcct
     8221 gaaccagcgc atcattgatc ttcgcgagct ccgcgcgatc gcctttccgc acagcctcct
     8281 gccgatcttt ctccacgcgt tctgcttctt ttgcaaaatc gtcgatcgcc gacgtcatcg
     8341 ctttgtcatc gattgacgac gcgagattgc ggatacgcgc gagtctcatc gcctcagtaa
     8401 agaagtcgcg aatctgatgc gcgtagtcag tgtaatcaaa cggaagccca tcagcgtccg
     8461 cgagacgcat cgccagcgtg ccccacaact gcgccgccgc cacgtgataa gtgaagccag
     8521 gatcgccaaa gtggtccatc cagtaaaacg agtcgtacgc cgagtggtaa acaccgtagt
     8581 cgccattgaa gcccatgtca gttgacgcca caccgaggtg ctgcagaaac ggcgtataat
     8641 cagagcccga gccgagtgca gcaatgcgcg cttcatgcat atcctgatcg ccttcgggac
     8701 gattctcgcg cgatcgatcc tgccactgtt gatagacact cttgcccgtc ttcggatcct
     8761 taacatcgcg cgtcgcgccg cgcataagct tccataacga cggcaccgac gaagcaccga
     8821 agtgtggtcc agagacagcc gcatcgagat tcagatacgc aaccgctttc tgtcgcagct
     8881 cgtcagccat ctcttcggcc cattcagtcg agccgatgag tccatactcc tccgcgtccc
     8941 aactacaaag gaagatcgtg cgtcgcggct tccaaccctg tttcaacaac tcaccgaatc
     9001 cgtggcccac ttcgagcatc gccgaggtac cgctgtttgg atcgaccgcg ccaaacaccc
     9061 acgcatcacg atggttaccg agcacgaccc agcgatcctt ctcttcgtta ccgtcgatac
     9121 gtgcgacgac gttccagatc gtccgcagct tgtagtccat gtcagtcttc aaacggagct
     9181 tcacgtcgtt cgtgccgccg acgtgataag cgaacggcaa gccgccctga aaaccgcgtg
     9241 gtcgcaacgg tcctttgagc tgcgagagga tcgtgcgcgc cacgtcgtaa gcgatcggct
     9301 gcacgggaat gcgcgtgaga tcggtcgctt cttccggttt caaacgagga gtgccggcga
     9361 tcgcgggctt gccgggcgtc aacggatcgc cgggataatc aaacaggtat tgcaccgagc
     9421 cgcgctggcc cgacgcaacc ggacgccacg gtcctttcgg aaacacatcg ccctgcatgt
     9481 aaccgtcgtc ttccggatcg gagtagatga tgcaaccgat cgcgccgttc tgttccgcca
     9541 ctttcgcctt cacgccgcga aacgagttgc cgtagcgcac gatcacgatc ttgcctttca
     9601 cgtcgacgcc ggctttcttg agatcttcgt agtcgttcgg cagaccgtag tttgcgtata
     9661 cgaccggtcc ggtcacgtcg ccgctggcgc tgaagccgtt gaagagcggc gagatcttcg
     9721 gatgtgacga cgatggatcg ccgggcactg gtgcttcctt gacgctgagt ggctggcgac
     9781 gcgtggtaat gagttcgagc acagtcgggg cgtttgggta gttgagccag acttcatact
     9841 ctttgagatc ggctgatagt ccaaagctgc gcagttgatc gcgaacgtag acggcggtgt
     9901 tgtagtcttc tttagtgcca gcgatgtgcg gctccacagt aagacgacgc aggtgttcgc
     9961 gcgcggattt cggatcagga acggcgcgaa actgttcttc ccagcgacgc tcggctgtcg
    10021 agcgttcggc gctgaagccg tcgattgctg tttgagcgaa cgttaccggt gttgtaagcg
    10081 taatgacgac aagtagaaag agaaccagta tagttttcat agcgcaggag gccagttaat
    10141 agatggacag cagatactat ccgggtgttg aagccgatgt tccgcaactc agtacggagc
    10201 ttcgcaatgt ttttgacagt gagtttgagg tgcagacgtt ccaggtttcg aacaccagca
    10261 ttttccaggc gcgtaagtcc agcacgctaa gagatctgac gggactctcc tcggccctca
    10321 cgatcaagat cacaccagag cccggcggca cacgcgtaga aatcgggatg cagaagtggt
    10381 tcgacaaagc ggcggttgcg gccgtcggtg ttgttgtctt tccaccgctg ctcgcactgg
    10441 cggcactcgg agcttactgg caatacaagc ttacggaaga cgcatggaag ataatcgaga
    10501 atcacatctc gcgcaaagcg ggcggctacg ttccaccgtt gccgggacgc tgcgggacgt
    10561 gcggcggcgc gaacatcgcg gagtctgatt tctgttcgtc gtgcggagcg aacttgcggg
    10621 ttggccggac ttgttcgaac tgcggtgcgc cccaaaaaga taacgccgcc cgattctgta
    10681 atcgttgtgg gaagccactt tctaattagc cacaaaaggc acaaaaagca caaagtgaat
    10741 attgatagga cgtcgtcctt tcagtaatca ttttgtgctt tttgtgcctt ttgtggctaa
    10801 tcatgcctga gagctcggac gggatcgatg cgtgcggcgc gtgaggctgg atacagcgcc
    10861 gccagaatcg atacgaagag cgcaaagagg atcgcgccca ccgacaaata aatcggcaac
    10921 gaaaagaaat cgataaacga agcgccctgt ggtttgagta tgaagcggta agcgatgcgg
    10981 ttcgcgatac cgtcgattcc ccacgctatc aacactccaa ctacgccgcc gacggtcccg
    11041 atcaccgccg cctcaacaaa gaagatcatt ttgatctcgc gatcctcggc gccgatcgct
    11101 ttcatgatcc cgatctcgcg cgtgcgttcg agtatcgaca tgatcatcgt gttcgcaatt
    11161 ccaaacgaag ccaccagcaa cgagattcct ccgagtagcc cgagcaccga gtcgatgatc
    11221 agaaacaccg tgcgtatctg ctcgatctca tcgacgatcg agaagcttcc aaaaccaagc
    11281 tccgtgatct tctgtcgcac ttccgtcaaa tgcttcggat cgctaacacg aacaaccgcc
    11341 gcttcgtaac cctcgttttg accttcactg agattaccgc cacgacgcgc gagcgcaagc
    11401 gccacctggc ccatcgggct gcgatgccga attgcccaat cgtgggccaa ctgtagcgga
    11461 atgtagatct gggcgcccgg gaataatcca cgcaaaccgc cctgcccgac gccttccttg
    11521 acctctccga ggacaccggc aatacggaac gtctgtattt caacatcggg attcgactca
    11581 tcgagccccg gatcgtcaag cggaataccg aagaagtttg gcgtgtcctc ctcctcttcc
    11641 tcctttttgt tctcagactt gttctcagat gggggcgcca gcaattcgag cgttttcccg
    11701 attgcgtcag cgggcttctc ataaccaaac tcgcgaatga atcgctccga gactactgct
    11761 tcaccggcgg taggagagga gatcatcttt ccggcggcga agtgttgaaa gcgagtcgcg
    11821 gcgttgggaa tgttggcgcc gccgatgttc gtgctccgca gtttgccgtt ggagcgaaca
    11881 aaaacgttca tgctgacact cggctcgacg tacgcaacac cgggtatggc ggcaatctct
    11941 ttgattcctt tgtcgtcgag aatacgtgtc ggcgatcgat ccgaacggcg atcgttacgc
    12001 ggttcgcctt ctggtccacg tgaagaacga tcgagtccgg cgagagtgct gaggccctga
    12061 ccgaagactt gtatctcgtt gaagaagtcg agcgctttga agcggtcgag catattgcgc
    12121 tgcagaccga gcccgaacga catcatcgtc acgatcaccg cgacgccgac gatcacgccc
    12181 atcgtcgtca ggatcgcgcg cagctttgcc tgtcgcaaat tccgcagcgc gagatgaagg
    12241 atgtccagga agctcatcga aaacctttgg acaggattta caagattttc aggatttaca
    12301 agatctatcc tgtt
//
LOCUS       DASSFQ010000276         7465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_732583, whole genome shotgun sequence.
ACCESSION   DASSFQ010000276 DASSFQ010000000
VERSION     DASSFQ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732583"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1676
                     /locus_tag="VFH96_09995"
     CDS             33..1676
                     /locus_tag="VFH96_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974345.1"
                     /translation="MTTPSGISSASKIKSPIRIAELVSSALIVAFFFWRLQFSTSAIC
                     CGDFDGYYHIKWTQTLWEAIKGGHFPQFPWLPLTTLNPKDYVDHHLLFHIFQIPFATS
                     SDPRLGAKISAILFGSLAVLSCYWLVLRYRIRYSFVWQIALLGCSAPFLFRMNMAKAP
                     PFAIIYLVIAIHLFFKRKYWPLLPLALVFTWTYDLFVLLIMAAVFWSAAIAITEKRFE
                     WRPLVYVIAGCVLGLIINPYFPHNLQLLVEHMKIKLTASDFDTKVGSEWYPYDSWEFL
                     GNSAVACIAMVVGYLFFEPTERRRGQYPIFFLLLSTALMIMTARWKRIAEYWPPFAVL
                     FAGFSLRPWLEGYRPYLTRLPADVQEELEPFLDREVPPPEKEEVSTWRDVIRVVAVTT
                     VAVVLSAFTLFNVYVTKQEISQSEPHDFYRKGAEWMRTHVAPGQLIFNTDWDDFPRLF
                     YFDPTHTYVSGLDPSYLYDKNPELSRLYESITLGHEEDPGPLIRDRFGARYVFSDTTH
                     HDFYEHARNSGWFDIVYEDADCVIMYIRDQKIEEGLEAP"
     gene            1673..2074
                     /locus_tag="VFH96_10000"
     CDS             1673..2074
                     /locus_tag="VFH96_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974346.1"
                     /translation="MRLISVLILGMLVVSCGNGISSRQGGFPSGVTNTNSASPPPAAS
                     PTASLTAFEQDLQYIRNGQYTYVWVFSRKDGKPLEPADSIFLRTNAPQVVDWVTTDGG
                     KKVIAGTNFNLEEGNLEVLKKRYVAEDYSNK"
     gene            complement(2103..3143)
                     /locus_tag="VFH96_10005"
     CDS             complement(2103..3143)
                     /locus_tag="VFH96_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6974347.1"
                     /translation="MKAVIFRKHGGPEVLEYTDVPEPQIRANEVLVEVKACALNHLDI
                     FVRNGLPGIEIPLPHILGCDVAGVVREAGELVTWVKPGDEVMVQPGVSCGHCEACLSG
                     QDNLCREYDMIGYRRDGGYAELVAVPGVNIIPKPAELSWAEAAALPLVTVTAWHMLVT
                     RANVQPGEDVLVHAAGSGVGSVAIQIAKLRGARVVTTASSDEKLAKARELGADETINY
                     TRDDWPKEVRRLTGRKGVDVVVEHTGAATWPGSIASLKNNGRLVTCGATSGFDARTDL
                     RQVFYRHLTLLGSFMGSKAELLEALKFVAQGKIRGVVDRVLPLSEARQAHELIEDRAQ
                     FGKVILATDLTD"
     gene            complement(3140..4042)
                     /gene="lipA"
                     /locus_tag="VFH96_10010"
     CDS             complement(3140..4042)
                     /gene="lipA"
                     /locus_tag="VFH96_10010"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET6974348.1"
                     /translation="MARELRVISNNQKVRQPKPEWLRIRLGDPTNQNQVLQLIEGLNL
                     HTVCQEARCPNIFECWSDRTATFMLGGDICTRHCGFCAVGKGQPGALDPEEPRHVAEA
                     VGHLNLAHAVITSVNRDDLPDGGAAHWAETIRQVRAANPDCKVEVLIPDFNGDEAALN
                     TVLDAEPDVLNHNTETIARLYRRVRPDAVYQQSLTLLQRAATRRDRENRGMLTKSGIM
                     VGLGETFDEVVDLMRDLRSVSCDIMTIGQYLQPHARRLPVERYVTPEEFAKWREIGMS
                     LGFHHVESSPLTRSSYHAREQAAK"
     gene            4131..4715
                     /locus_tag="VFH96_10015"
     CDS             4131..4715
                     /locus_tag="VFH96_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974349.1"
                     /translation="MKSLLVACSLLLLFAFSTNADVPKPKTPDKTAKQVLHTGLEIVP
                     DPKVYEAKLQISQSGFNNLRAALDGGAGSSPVVGGIVFSAPRTIVAGLLMFMAVSVAG
                     VLIARSSSVGRAQKTIGAALLVAVLLGAAAIITRGNAGPPPSWRWRNLPQALAEGKST
                     TGGVDVEIVPDDQMDGRTMRLLIPLKKQNSPGEE"
     gene            4716..5750
                     /locus_tag="VFH96_10020"
     CDS             4716..5750
                     /locus_tag="VFH96_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974350.1"
                     /translation="MQNATVGVALRGHPSREIINASIPFSAIVVLSVIAAAAFASWLP
                     LQVSIVTLFLFAGPHNWFELRYFLMRLPVRFGRSRNFFLTAFAGLGVLTLTYVSLPFL
                     YNATLWTEEAWYVVIGSWNTALLVWLALLVWLRSKQKPRANWKWTIPVALTLCSLNWL
                     GPDLFSLAIVYVHPLIALWFLDRHIRRTRPEWLRAYRRCLWLVPVVLAVMIWQLSQTT
                     PLADNNGLFWRITQHSGAEVLPQVSSHMLVSVHLFLEMLHYGVWIVALPLIGRIGSAR
                     FWEVRTVPIARHPKGFPRIVAGLMIVALVAVGVLWFGFSVDYSTTRDIYFTVAIAHVL
                     AEAPFLLRML"
     gene            5750..6100
                     /locus_tag="VFH96_10025"
     CDS             5750..6100
                     /locus_tag="VFH96_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974351.1"
                     /translation="MSPFDLWQFLPVGYLLTIAIETPILFFGLSPHHSKRRRIFAGLW
                     LTACTYPIVTLVLPLLLVDHSRAFYLLIAEVFAPVAECALFWLAFGKEATWRDFVVIV
                     VANIASFLIGEVVF"
     gene            complement(6110..7063)
                     /locus_tag="VFH96_10030"
     CDS             complement(6110..7063)
                     /locus_tag="VFH96_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET6974352.1"
                     /translation="MLTLDQINEARHRIASRIHRTPVLTSRQFNEAAGKEVFFKCENF
                     QRAGAFKIRGATNKIQSLTSDEKRQGIVAFSSGNHAQAVALAGREAGVHVLVAMPEDA
                     PAAKVAATRGYGAEVVFYDRHKQDREAFALDLAERERLIMVPPYDDYLILAGQATCGL
                     ELMEEIPDLDCVLAPCSGGGLFAGVASAAKSMNPAIRCFPVEPDTADDTRQSFLKGER
                     VTIPPPPTIADGLRVQIPGKLTFPIVQKLAEDVLTVTDEEILSTLRFLLFRMKILVET
                     SGATAAAAVLFKKLPPDVRRVGVILSGGNIDPDLLQSVINP"
     gene            complement(7057..>7465)
                     /gene="lipB"
                     /locus_tag="VFH96_10035"
     CDS             complement(7057..>7465)
                     /gene="lipB"
                     /locus_tag="VFH96_10035"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET6974353.1"
                     /translation="QIVGYPIINLSPDREDVHRYVRDLEEVLIRTMSDFGIDAFRIEG
                     LTGVHTPRGKIAAIGVHISRWVTTHGFALNVNTDLSFFDLIIACEGEPVTSMEVLLGD
                     EIDLSAVEDRITEHFSNIFSLSVFSVFICGLIC"
BASE COUNT         1473 a         2118 c         2106 g         1768 t
ORIGIN      
        1 ccaactgtga gtaaaaacta gttgagcgat tgatgacgac cccgtccggc atatcctccg
       61 cgtctaaaat caagtccccc atacgaattg ctgaacttgt atccagcgcc ctcatcgtcg
      121 ccttcttttt ctggagactc cagttctcaa cgtcagcaat ctgctgcggc gatttcgacg
      181 gttactacca catcaaatgg acgcaaacgc tctgggaagc gatcaagggc ggtcatttcc
      241 cccagtttcc ctggcttccg ctcaccaccc tgaacccaaa agactatgtg gaccatcacc
      301 tgctgttcca catttttcag attccgtttg ccacgtcttc agacccacgt ctcggcgcta
      361 aaatctcggc gatactcttc ggcagcctgg cagtgctgtc gtgttactgg ctggtgctgc
      421 gctatcgaat tcgttattcg ttcgtctggc agatcgcgct gcttggctgt tcggcgccgt
      481 ttttgtttcg catgaacatg gcgaaggcgc cgccgtttgc gatcatctat ctcgtcatcg
      541 cgattcacct gtttttcaag cgaaaatact ggccgttgct gccgctcgcc ctcgtcttta
      601 cgtggaccta cgacttgttt gtgctgttga tcatggccgc ggtcttctgg tcggctgcga
      661 ttgcgatcac tgagaagcgg tttgaatggc gtccgctggt ttacgtaatc gccggatgcg
      721 tcctcgggct catcatcaat ccttacttcc cacacaatct gcaactgctc gtcgaacaca
      781 tgaagatcaa gctcacggcg tcagacttcg acacaaaagt cggatctgag tggtacccgt
      841 acgactcgtg ggagtttttg ggcaactccg cggttgcgtg tatcgcgatg gtcgtcggtt
      901 atctcttctt tgagccgacg gaacgcaggc gcggtcagta tccgattttc tttctgctgc
      961 tctcgacagc gctgatgatc atgacggcgc gctggaaacg gatcgcggag tactggccgc
     1021 cgtttgcagt gttgttcgcc ggtttttcac tgcgaccgtg gctggaaggt tatcgcccgt
     1081 atctgacgcg actgccggcc gatgtgcagg aggagctcga gccgtttctc gatcgtgaag
     1141 tgccgccgcc tgaaaaggag gaggtgagta cgtggcgtga tgtgattcga gtggttgcag
     1201 tcaccaccgt ggcggtcgtg ttgagcgcct ttactttatt caacgtatac gtcacgaaac
     1261 aggaaattag ccagagtgag cctcacgatt tctaccggaa gggcgctgaa tggatgcgca
     1321 cccacgtagc accgggacag ttgatcttca acacggattg ggacgacttt ccgcgtctct
     1381 tttacttcga cccgacacac acttacgtct ccgggctcga tccgagttat ctctacgaca
     1441 agaacccgga actgtcgcgt ctttacgaaa gcatcacact cgggcacgaa gaggatccgg
     1501 ggccgttgat tcgcgatcgc ttcggcgcgc gttacgtgtt ttctgacacc actcatcacg
     1561 acttttatga gcatgcgcgg aatagcggct ggtttgatat cgtttacgag gacgcggatt
     1621 gcgtaatcat gtacattagg gatcagaaaa ttgaggaagg attggaagcg ccttgagact
     1681 tatttcggtt ttgattcttg gcatgttggt cgtgagctgc ggaaatggta tttcgtcgcg
     1741 ccagggtggt tttccgtcgg gcgttacgaa taccaactca gcgtctccgc cgcccgctgc
     1801 ttctccaacc gcatcgctca cggccttcga acaggatctc caatacattc gcaacgggca
     1861 atacacttac gtttgggtct tctcacgcaa agacgggaag ccgctggagc cagccgacag
     1921 catcttcctc cgcaccaacg cgccacaggt tgttgattgg gtgactaccg atggcggtaa
     1981 gaaagtgatt gccggaacga acttcaatct cgaagagggg aatttagagg tcttgaagaa
     2041 aaggtacgta gcggaggact actcgaataa gtaacacgga ttaaacggat aaaaatccgg
     2101 atctaatccg ttaaatctgt ggctaaaatg actttcccga actgagcccg atcctcaatc
     2161 aactcgtgcg cctgccgcgc ctcgctgagt ggcaatacgc gatcgacaac accacgaatc
     2221 ttcccctgcg caacgaactt cagcgcctcc agcaactcag cctttgagcc catgaacgat
     2281 ccgagcagcg tcaaatgacg gtaaaacacc tgccgcagat ccgttcgcgc atcgaagcca
     2341 ctcgtcgcac cacaagtcac gagtcgtcca ttgtttttga gtgacgcgat cgatccgggc
     2401 cacgtcgcgg cgccggtgtg ctcgaccaca acgtcaacac ctttgcgccc ggtcagtcgc
     2461 ctcacttcct tcggccaatc gtcacgcgtg tagttgatcg tctcatcagc cccgagctca
     2521 cgggctttcg caagcttctc atctgagctg gccgtcgtga ccactcgcgc gccacgcagc
     2581 ttcgcaatct gaatcgcgac tgaaccgacg ccgctgcccg ccgcatgcac aagcacgtct
     2641 tcacccggct gcacattcgc gcgcgtcacc agcatgtgcc aggcggtgac agtcacgagc
     2701 ggcaatgccg ccgcctcagc ccaactcagc tctgccggct tgggaatgat attcacgccc
     2761 ggaaccgcca ccaactccgc ataaccaccg tcacgacgat aaccaatcat gtcgtactcg
     2821 cgacacagat tgtcctgccc gctcaggcac gcctcgcaat gcccgcacga aacgccgggc
     2881 tggaccatca cttcatcgcc cggcttgacc cacgtcacga gctcgccggc ttcgcgcacg
     2941 actcctgcca catcgcaacc gagaatgtgc ggcagcggta tctcaatccc cggcaaaccg
     3001 ttgcgaacaa aaatgtcgag gtgattcaac gcgcacgctt tcacttcgac gaggacttcg
     3061 ttcgcgcgaa tttgcggctc gggcacgtcc gtgtactcga gcacttccgg cccaccgtgt
     3121 tttctgaaaa tcacagcttt cattttgcgg cttgctcgcg tgcgtgatac gaggaacgcg
     3181 tgagtggact tgattcaacg tggtgaaatc cgagcgacat gccgatttcg cgccacttcg
     3241 caaactcttc cggcgtcacg taacgctcga ccggcaaacg acgcgcgtgc ggctgtagat
     3301 actgtccaat cgtcatgatg tcgcacgaaa ccgagcgtaa gtcgcgcatc aaatcgacga
     3361 cctcatcaaa cgtttcgccg agcccgacca tgatcccgct cttcgtcagc atgccgcggt
     3421 tttcgcgatc gcgtcgtgtc gccgcgcgct gcaacagtgt caacgactgc tggtagaccg
     3481 cgtcaggcct gactcgtcga tacagtcgcg cgatcgtctc ggtattgtga ttgagcacgt
     3541 ccggctcggc gtcgagcacg gtgttgagcg cggcttcgtc gccgttgaaa tcgggaatga
     3601 gaacctcaac tttgcaatcc ggattcgcgg cgcgcacttg gcgaatcgtt tcggcccaat
     3661 gcgcagcgcc gccgtcaggc agatcgtcgc gattaaccga cgtgatgacg gcatgagcga
     3721 gattcagatg gcctacagct tcggcgacgt gacgtggctc ttccggatcg agggcgcccg
     3781 gctggccttt gccgaccgcg cagaagccac agtgacgtgt gcagatgtcg ccgccgagca
     3841 tgaaagtggc ggtacgatct gaccaacact cgaaaatgtt cggacagcgc gcttcctggc
     3901 agacggtgtg caggttaaga ccttcgatca gttgcagcac ctggttctgg ttagttgggt
     3961 cgcccaggcg gatgcgcagc cattcgggct tgggttgacg gaccttttga ttattgctga
     4021 ttactcggag ttcgcgggcc aaattgttta cctctgcaag gattttacag tataaggtgc
     4081 gaagttcaga attcagccac ggttagatct tcctccaaag gagcgcgcca atgaaatctc
     4141 ttctcgtcgc ttgttccctt cttctgttgt tcgcattttc aaccaacgcc gacgtcccga
     4201 aaccgaaaac tccagataag acggcgaaac aggtcctgca cacgggcctt gagatcgtcc
     4261 cagaccccaa agtttacgag gcgaaattac agatctccca gtctggcttt aacaacttgc
     4321 gcgcggccct cgatggcggc gctggcagct caccggtcgt tggtggcatc gtgttcagcg
     4381 cgccgcgaac gattgtcgcc ggattgctga tgtttatggc tgtttctgtg gcgggcgtgt
     4441 taattgcgcg ttcgtcgagc gttggccgcg ctcagaaaac gatcggagcg gcgctgctcg
     4501 tggcggttct gctcggtgca gcggcgatca tcacgcgtgg caacgccgga ccgccgccat
     4561 catggcgctg gcgaaacctg ccgcaggcgc tcgctgaagg aaaatcaacc acgggcggtg
     4621 tcgacgttga gatcgttcct gacgaccaga tggatggccg cacgatgcgg ctcctgattc
     4681 cactcaagaa acagaacagc cccggcgagg aataagtgca gaacgcaact gtaggggtgg
     4741 ccctccgtgg ccacccgtcg cgtgaaatca tcaacgcatc gattccattt agcgcgatcg
     4801 ttgtgctatc ggtgatcgcg gcggcggcgt tcgcgagctg gttgccgctg caagtatcga
     4861 tcgtgacgct cttcctcttc gccggtccac acaactggtt cgagctgcgc tactttctca
     4921 tgcgactgcc ggtccgcttc ggcagatcgc gcaatttctt tttgacagcg tttgccggtt
     4981 tgggtgtgct gacactcact tacgtctcgc ttccatttct ctacaacgca acactgtgga
     5041 ccgaggaagc gtggtatgtg gtcatcggtt cgtggaacac tgcgctactt gtttggctgg
     5101 cgctgctggt gtggttgcga agcaagcaaa agccacgcgc aaattggaaa tggacgattc
     5161 cggttgcgct cacgctgtgt tcgttgaact ggcttggtcc agacttgttc agtctcgcga
     5221 tcgtttacgt ccacccgctg atcgcgctct ggtttctcga tcgacacatt cgtcgcacac
     5281 gacccgaatg gctgcgcgct taccggcgtt gtctctggct cgtgccggtc gtgctggcgg
     5341 tgatgatctg gcagttgtcg cagaccacgc cgcttgctga caacaacggt ctgttctggc
     5401 gcatcacgca acactcgggc gcggaggtgt tgcctcaagt ttcgagtcac atgctggttt
     5461 cagttcactt gtttctcgag atgctgcact acggtgtttg gatcgtcgct ttgccgctga
     5521 tcggacggat cggaagcgcg cgtttttggg aggtgcgtac cgtgccgatc gcgcgacatc
     5581 cgaaaggatt tccgcggatt gtcgccgggc tgatgatcgt tgctcttgtg gctgttggtg
     5641 tgctgtggtt tgggttttca gttgattact ctacgacacg agatatctat ttcactgtgg
     5701 caatcgcgca cgtgctcgca gaagcgccgt ttctgctgag gatgctttag tgtctccgtt
     5761 cgatctctgg cagttcctgc cggttggcta cctgctcacg atcgcgatcg aaactccgat
     5821 actcttcttt ggtctctcac cgcatcattc aaaacggcga cgcatctttg caggcttgtg
     5881 gctaacggcg tgtacttatc cgatcgtcac gctcgtgctg ccgttattgc tggtggacca
     5941 ctcgcgtgct ttctatcttt tgatcgctga agtattcgcg ccggtggctg agtgtgcgtt
     6001 gttctggctc gcgtttggga aggaggcaac gtggcgcgac ttcgtggtga tcgtcgttgc
     6061 gaatatcgcg tcgtttctta ttggtgaggt tgttttttag ccgcggatct cacggattga
     6121 ttacggattg aagcagatcg ggatcgatgt tgccgccgga caggatgacg ccgacgcgac
     6181 gcacgtcggg aggaagtttt ttaaatagaa cagcggcggc ggcggtggcg cctgaggttt
     6241 cgacgaggat cttcatgcgg aagagcagga atcgaagggt cgagaggatt tcttcgtccg
     6301 taactgtgag cacatcctcg gcgagtttct gcacgatcgg aaatgttagt ttgccgggga
     6361 tttgaacgcg cagaccgtcc gcaatcgtcg gtggaggtgg aatcgtgact cgctcgcctt
     6421 tcagaaatga ttgtcgcgtg tcgtcagcag tgtccggttc gaccgggaaa caccggatcg
     6481 ccgggttcat ggatttagct gcactggcaa cgcccgcgaa taaaccaccg ccgctgcagg
     6541 gcgcgagcac acaatcgaga tcgggaattt cttccatcag ctctagaccg catgtcgcct
     6601 ggcctgcaag tattagatag tcgtcgtacg gcgggaccat gatcagccgc tcgcgctcgg
     6661 ctagatcgag tgcgaaggct tcgcggtcct gtttgtgacg atcgtagaag acgacttcgg
     6721 cgccgtaacc gcgcgtcgct gcgactttcg ccgccggtgc atcttccggc atcgctacga
     6781 gcacgtggac ccccgcttcg cgaccggcca gcgcgacggc ctgcgcgtga ttgccggatg
     6841 aaaaagcgac gatgccctgg cgtttctcgt cagacgtgag cgattggatc ttgttcgtcg
     6901 cgccgcggat cttgaaggct cccgcgcgct gaaagttttc gcacttgaaa aacacttcct
     6961 tgcctgcagc ttcgttgaac tgtcgcgaag tcagtacggg cgtacggtga atgcgggagg
     7021 cgattcggtg tcgtgcttcg ttgatttgat cgagagtcag catattaggc cacagataaa
     7081 cacggaaaag acggataaag aaaagatgtt ggagaagtgc tcggtgatgc gatcttcgac
     7141 tgcgcttaaa tcgatttcgt caccgagtaa gacttccatc gacgttacgg gttcgccttc
     7201 gcaggcaatt atcagatcga aaaaactgag atcggtgttt acgttgagcg cgaagccgtg
     7261 cgtcgtcacc caacgcgaga tgtgtacgcc gatggccgcg atcttcccgc gcggcgtgtg
     7321 aacaccagtc agtccctcga tgcgaaaagc gtcgatgccg aagtctgaca tggtccggat
     7381 cagcacttct tcaagatcgc gaacgtagcg atgaacgtct tcgcggtccg gactgagatt
     7441 gatgattggg taaccgacga tctga
//
LOCUS       DASSFQ010000277        16247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1163881, whole genome shotgun sequence.
ACCESSION   DASSFQ010000277 DASSFQ010000000
VERSION     DASSFQ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16247
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163881"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VFH96_10040"
     CDS             complement(<1..1794)
                     /locus_tag="VFH96_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6974354.1"
                     /translation="MIKRFQVLPIFFLLLLPVFAQQSDPSLLTLDSMFTYRIRPLGPV
                     QWQTDGSGYLALEPSTTKKDFLDIVRYDVSTGDRTVKVSAEKLTPAGAAEPLAVEEFT
                     LTPDEQKLLIFTNTERVWRSNTRGDYWVLDLKANTLKKLGGTDAKPSSLMFAKFSPDG
                     QRAAYVRENNIYVENLSGDGKITKLTNDGSRYIVNGTFDWVYEEELFCRDGFRWSPDS
                     KQIAYWQLNSEGVKEMTLINNTAALYPVLTTFPYPKAGETNSAARVGVVDANGGPTRW
                     IEVPGDPRNNYLPRMEWAASSDELIIQQLNRRQDTNTVMMGDARTGKVRPVLTEKDEA
                     WVDVAWGDIDWDKTGLGRGDVEWIDNGKRFLWTSERDGWRHIYSVSRDGSDVKTITPG
                     NYDVISVARVDAQNGWMYFIASPENAAQKYLYRVKLDGAATGKEERLTPANLPGTNGY
                     NISPRGDFAIHDYSTFNRVPVIDVVRLPQHSVARTLIDNKQVQDRLAQLKQTPSEFFK
                     VDIGDGVQLDGWMMKPPDFDPQKRYPVLFYVYGEPWGQTVLDAWFGRNRLWHQMLAQQ
                     GYIVMSVDNRGTPAPRGRAWRKIIYRKMGIVN"
     gene            complement(1891..3852)
                     /locus_tag="VFH96_10045"
     CDS             complement(1891..3852)
                     /locus_tag="VFH96_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6974355.1"
                     /translation="MENTSALLLNRLIDASVDGIFAFDRDCRITAWNLAMQRISGFSR
                     EAVLGKRVGDIFPFLREEGNDSCFLAALEGKESVSEYHPYGPAETGVFESHYSPLLDE
                     QNKIGGGIAVITDVTARKHAEGAAETAYKQLAFHVESSPLAVVEWDSDFRVSRWSASA
                     ERLFGWKAEEVIGKHVNEWRFVFADDEDAVALVTNRQREGVEVHGILRNRNYTRGGAV
                     LFCEWYNSVLHDDRGKLISVLSLVLDVTARQSAEDARSAALSREQDARKHAEEADRLK
                     DEFLATLSHELRTPLTSILGWASMIRNGEVEGSNASRAIETIERNARSQARLIDDLLD
                     VSRIITGNLRLDLHPLNLGPIVEAALDALRPTADVKGIKLQTRFEPSECLVKGDANRL
                     RQVIWNLLSNAIKFTTRGGSVSIYLKCVEATARLKVSDTGDGISAEFLPYVFDRFRQA
                     EGSISRKQGGLGLGLAVARHLVELHGGTIKAESDGLGRGSVFTVDLPMTQERRDPARA
                     EERKREVDRRHSRKGVVRLDGVHVLLVEDDDDSRKLLGTMLRRYGARVTSTKSAAEAL
                     EVFAGELPDVMISDIGMPDQDGYELVRRLRSLPMEKGGATPAIALTGYASRKDRERAL
                     GSGYQKHMAKPIEQAELIAAIAALVARGD"
     gene            complement(3887..5119)
                     /locus_tag="VFH96_10050"
     CDS             complement(3887..5119)
                     /locus_tag="VFH96_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6974356.1"
                     /translation="MNKLGLIFVFLTATVTVTAQEWTQWRGPARDGVVTTKNAPAAWP
                     ESLKRTWRVEIGEGYSSPVVSGGRAFVHGRRDPEEIVASINLADGKVLWEQKYQAEFK
                     KNQYAVSMAKGPNATPLIAGNRLFTLGVTGILNAWDTTTGKQLWSKDFSNAIDTSKLF
                     CGTAASPLLVNGRVVVQVGSDIHGGQVLALNPATGATEWEWKGLGPGYASPVMIEAGG
                     SQQIVTMTEGSIVGLDGKTGKELWSVTFPDEWHENITTPLWTGTHLVVSGTRQGTHAY
                     TLAQNAGKWQATEAWKNPDIAMYMSSPVFGDGLIYGHSSKRKGQFFAIDAKTGAVRWT
                     TEGREGEHASLLLTPQHVVFLTNGADLIVAKRGTAAFTVERRYEVAEASTFAMPVLLG
                     SNILVRDATSLMLLTAGK"
     gene            complement(5242..6054)
                     /locus_tag="VFH96_10055"
     CDS             complement(5242..6054)
                     /locus_tag="VFH96_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974357.1"
                     /translation="MSTANHIWQPLWQLPSRADLIFSFAKRELLGRYKGSALGIAWAV
                     LTPVVMIAIFTFIFAGIFGARFGANDSHWDYALYLFCGLLPWSMFQESVQQSANTILA
                     HSNLVKRVVFPLEALPAAQVFAALGSQLFATIALLIATIIIRQRLELTSLFLPVLLIP
                     QLLFALGAAWLVASLGVFLRDITQGIMLLLMAWMYLTPIIYPEAIVPERFRTFINLNP
                     FTSLIRSYRHIFLDGTTPDWRGLAYFTTFALLLFLFGYWWFARTRKSFADVV"
     gene            complement(6112..7107)
                     /locus_tag="VFH96_10060"
     CDS             complement(6112..7107)
                     /locus_tag="VFH96_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6974358.1"
                     /translation="MKQSLLSYLICPSCEVPLDLSVTKTEDGEIIEGELRCTSCSISF
                     PITAGIPRFANLDHVESDKRATAAGFGWQWQHFTQEDERYAEQFLGWIAPVTPEFFRD
                     KIVLEGGCGKGRHTQLAARWGARDVIGIDLSVAVETAFAATRSLPNAHIIQADIYHLP
                     LARKFDYAFSVGVLHHLPDPRGGFLALAGKVKPGGHLSAWIYGAENNEWITRFVNPVR
                     EKITSRIDQRALLQLSKLPAAGVYLATKLVYGPLNRRGRSLAKRLFYNDYLSAISNFG
                     WREQHTIVFDHLVAPTAFYISRDDFETWWRDIDARDVEISWHNKNSWRGLGKISC"
     gene            complement(7199..9352)
                     /locus_tag="VFH96_10065"
     CDS             complement(7199..9352)
                     /locus_tag="VFH96_10065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET6974359.1"
                     /translation="MANRGTMRGAFLDLAAREVQMQYRVALITSLALFFSLGTRERLE
                     IRDSFEFEPSIQTVRKIRWPKKTIEVALSTSLLSPGSNIKPDSDVVGAARRALARWAS
                     LSNINFIVTWSPAASVSPAEAGDGISLLTIAVTPENEAFNSEATTGRTRVFFDPETGN
                     IAEADISINPRPRAEDGTELQFSTDGTPGTYDLEATFTHEIGHLLGLDHSSVLSSTMQ
                     GRQAFNGTFGLPALTERTLSEEDRQKIRGLYGSNLKLGRIEGRLVDNRTAGALTPLNG
                     VSVWAESVVNGRLIASDVTAEDGTYRLEGLTPGQYRVVVASSVDAAQKFRSFELSSQV
                     VVKADAATPLNSSLVQPQASVLNPKMIGLNAELSTVALPLTPGKRVKIYFGGEGVDQV
                     PGTSIAVNSPFFTVDPATLTREQIAAPFPIVSIEVQVAPNAPFGDYTLRLQSNSGETA
                     YVPGAITIDPGVASMLSNPVDDFRFFVTQQYTDSTGREPDSATLDKLTAQLTGCNLRS
                     DCLRTRRIDISTNLLIENELPATGVFLYGLYSTGLGRQPRYAELETDRALVLNQKSEL
                     ESVRLALVNAFVERAEFKRRFPATMKPSEFVDSLLTTMAQTTGVDLTSERSLLIGLLD
                     DSANGRAAVLTRLASDQRVVDANYNHALVLFQYFTYLRRNPDEAGYNTWLNTLKTKPL
                     RDPDAARSLVCNFLTSAEYQSRFTMNPTHNSRECN"
     gene            complement(9523..12018)
                     /locus_tag="VFH96_10070"
     CDS             complement(9523..12018)
                     /locus_tag="VFH96_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974360.1"
                     /translation="MKQVFWFLCAIIVPVALGGSLASVSAQSTSPTPDPFTVQLTSTP
                     TSAFNSIVSDISANGRFVVFTSNGNVATVNPNNVDGNREIFLADYAQRRIFQITNTKN
                     VPVVTPSPSPTPTPSPTPTPSPTASPSPTPTPQPTPADRSKVKVEIDNRTPMISLAPT
                     LVGGVRSYIIVFSSNAPDPKFFDGSDSDTLDADANSEIWIYRVPAVADVDLTLGADLP
                     FQDLSTGTFDPVTGNTPASRAPTPGSTSNSPFFADDNREPTISDDGGIIAFISTRNLV
                     PAVGNADFNPELFFYTVATKTFTQGTHTQDAVAGVGLIFSTNPMLSSDGSKVAFMSSA
                     NLATNNADNNAEIFTTTFSGGTVGAFFQVTRTLNNVANTNVLSPGRRMSRNGGLIAFE
                     SRATDPKSNAAPAANSVLGNWVYTVGTDTFNEVGTRPPISDFARFPTFTDYDASGNPS
                     TLVFTSFLNFRPNGTFPDSTATAPEGLNTDNSAEIYITPLPITSTNTFTRITNITNLQ
                     TFGGTRPLASETRKRMTFSLGGAEIGGGNGDGSTEVFYLITPTITSQSSAVLSFFTGA
                     SNMPVAAATPVPSPTPSPTPTPSPVPGTPAGLAPGELAIVRSTVVLAPSDLQSSGASE
                     TTRSPALPVELNGVSVSVNGAAAGLYFVGNTSKQINFVVPIGLASGLGTVAVNVLDAG
                     ANTDTLQRGLVQILFAQPDIFTTTADAGGRAAAINITNPMNRLAEPFSVTSDDGTGTQ
                     VPTKLELTVTGVRFAARTEVSVMVGTTNITGDAILSVQPNREMPGFDIITFTLPASLA
                     GAGDVPIQVTFTRGTQQTAVSRPADTAPHITIN"
     gene            12215..13240
                     /locus_tag="VFH96_10075"
     CDS             12215..13240
                     /locus_tag="VFH96_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET6974361.1"
                     /translation="MPKKALITGITGQDGSYLADYLLSLGYEVHGLVRRVALEAPETR
                     FSRIKHLLDRVTLHPASLESYASIFHVFSKHDFDECYHLAAQSFVAESFADGFWTMST
                     NINGTHYVLAALRELRPKCRFYFAGSSEMFGNVNEVPQKETTAFQPRSPYGISKVAGH
                     HLTRNYREAYGLFCVSGILFNHESPRRGFEFVTRKTTSTVARIKHGLANELRIGNLEA
                     KRDWGHAKDYVRAMHAMLQHSEPDDFVIATGETHSVRELCEIAFGEVGLDYREFVKVD
                     ERYYRPAEVDLLIGDPSKAREAIGWEPTYTFGELVKEMVASDLKALNEEFPKCPTNFS
                     LSRNPTS"
     gene            13198..14565
                     /locus_tag="VFH96_10080"
     CDS             13198..14565
                     /locus_tag="VFH96_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974362.1"
                     /translation="MSDKLQFVAQSDKLKHVGHQTFLTMPIAVRVDSVSKQYRIYDRP
                     ADRLKETLTRGHWKAHREFWALKDVSFEVEAGTTTGIIGPNGSGKSTLLQIITGTLEP
                     THGSVSIEGRVAALLELGAGFNPEFTGIENIFMNASLMGFSKAETEQLLPEIANFAEI
                     GDFIYQPLKMYSSGMYIRLAFAAAIAVAPQILIIDEALAVGDAVFQHRCTRRIKEMQE
                     NGTTILFVSHDPSAVRALCNRAVLLNGGQKIAEGTPADVLNRYQKIIMARQQAYEASS
                     TKTADQTGEFVERKTTPLSYVYRHGDRSAEVLEVEVLDNSLHPVELVETGEPVTVRIV
                     YVAHEDLDDVVCGFLIRNRHGIHIYGTNTELQEVPFERVRRGELTEVTFEFNCWLAPE
                     MYSLCVAVHSSAAVSFDWLDGCLFFRVMSATKMEGVVNLNATATTKRLARSSTDYTDS
                     VKQSV"
     gene            14588..16246
                     /locus_tag="VFH96_10085"
     CDS             14588..16246
                     /locus_tag="VFH96_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6974363.1"
                     /translation="MDSQIETDGLDLAQLMAQIRKDAETRKRSSLNHGDPARYRQLIV
                     QNFNAFLAPQISSLPPLKLQPELEKRDRYHVNDLLSFHDEVFVRNAYKIILKREPDDS
                     GMAHFLKNLRNGRYSKLDVLSALRFSSEGENVNVQIEGLSRRSVLRKMYRVPVAGYLL
                     RLAVAIARLPVLITNFRRLESHSMAQLERVAAHVNDAVAHLSQANRTHFESLQKQLND
                     LKGTVQLQINSLVREQEKFSQVQTVTRQNLSTTQSELSERIDLTTHHAREQDELYAAE
                     LQALRVQVEKQIDDLIERLQRSRMDLAQQETRLSRLLEGAVLTPSTPAQPWHPEQDHL
                     LDSLYFSLEDVLRGTTEQIKEEVKIYLPVLGNAGISSEILDVGCGRGEWLQVLREAGL
                     QARGIDTNRILVQQCKELSLDVEEREALEFLGSLPDGSLNAVTAFHFAEHLPLETLVR
                     FLDETGRTLKPGGLIILETPNPENLLVGSCNFYLDPTHKNPIPIPTMKLLLEARGFRC
                     EEVLKLHAVSSTKIEVKDQLTSHLNHFLYGPMNYAVVARKPESE"
BASE COUNT         3586 a         4510 c         4698 g         3453 t
ORIGIN      
        1 attcacaata cccatcttgc gatagatgat cttgcgccac gcgcgaccac gcggcgccgg
       61 cgtgccgcgg ttatcgacgc tcatcacgat gtaaccctgc tgcgcgagca tctggtgcca
      121 gagtcgattc cggccaaacc acgcatcgag cacagtctgt ccccacggct cgccgtaaac
      181 gtagaacaac accggatagc gtttctgcgg atcgaaatcg ggaggcttca tcatccaacc
      241 gtcgagttga acaccgtcgc cgatgtcaac tttgaaaaac tctgacggcg tttgttttag
      301 ctgcgcgagt cgatcctgca cctgcttgtt gtcgatcagc gttcgcgcga cgctgtgttg
      361 cggcaggcgc accacgtcga tcacgggaac acgattgaag gttgaataat cgtgaatcgc
      421 aaaatcgccg cgcggcgaga tgttgtagcc gttcgtaccg ggaagattcg caggtgtcag
      481 tcgctcttct ttgcctgttg ccgccccgtc gagcttcacg cgatagagat atttttgcgc
      541 tgcgttttcc ggtgaagcga tgaagtacat ccaaccgttc tgcgcatcga cgcgcgccac
      601 gctgatcacg tcgtaattgc cgggcgtgat cgtcttcacg tcgctgccgt cgcgcgatac
      661 ggaataaatg tgacgccagc catcacgctc gctggtccat agaaaacgtt tgccgttgtc
      721 gatccattcg acgtcgccgc ggccgagtcc ggttttgtcc cagtcgatgt cgccccacgc
      781 aacgtcaacc cacgcttcgt ctttttcagt caacaccgga cgcactttgc ccgtgcgggc
      841 atcgcccatc atcacggtgt tggtgtcctg acgacgattg agctgctgaa tgattagctc
      901 atcagagctc gcggcccact ccatgcgcgg caggtagttg ttgcgcggat caccgggcac
      961 ttcgatccag cgtgttggac caccattcgc atccacgacg ccgacgcgag cagccgagtt
     1021 ggtctcgccg gctttcggat acgggaaggt ggtgagcaca ggatagagcg cggcggtgtt
     1081 gttgatgagc gtcatctcct tcacgccttc ggagttgagc tgccagtaag cgatctgttt
     1141 gctgtcagga ctccagcgga agccgtcgcg gcagaagagt tcttcttcgt agacccagtc
     1201 gaaggtgccg ttgacgatgt agcgtgaacc gtcgttcgtg agttttgtga tcttcccatc
     1261 gccggaaaga ttttcgacgt agatgttgtt ctcgcgcacg taggcagcgc gctggccgtc
     1321 gggtgaaaac ttcgcaaaca tgagactcga aggcttggcg tcggtgccgc cgagtttctt
     1381 caacgtattg gctttgagat cgagaaccca gtaatcgccg cgtgtgttgc tgcgccagac
     1441 gcgctcggtg ttggtgaaga tgagaagctt ctgctcgtcg ggtgtgaggg tgaactcttc
     1501 caccgcgagt ggctcagcgg cgcctgccgg cgtgagtttc tcggcggaga ctttcacggt
     1561 gcgatcgccg gtgctcacgt cgtagcggac gatgtcgagg aagtcttttt tggttgttga
     1621 cggctcgagt gctagatagc cgctgccatc cgtctgccac tgcaccggcc cgagcgggcg
     1681 gatgcgatac gtgaacatcg agtccagcgt gagcagcgac ggatccgatt gctgcgcgaa
     1741 tactggaagg agtaagagaa agaaaatggg aagtacttga aatcgtttaa tcatttttat
     1801 tcctcatgtt agtcgttgac aagatttcaa gattttcagg attaacaaga tgaatcgtgt
     1861 gaatcctgaa aatcttgtaa atcctgtcta ctaatcacct cgcgcaacga gcgccgcaat
     1921 cgcagcaatc aattccgcct gttcgatcgg cttcgccatg tgtttctggt aacccgagcc
     1981 gagcgcgcgc tcgcgatctt tccgcgacgc ataacccgtt aacgcgatcg ccggtgtcgc
     2041 gccacccttc tccatgggca gcgatcgcaa ccgccgcact agctcgtaac catcctggtc
     2101 cggcataccg atatcgctaa tcatcacgtc cggtaactcg cccgcaaaca cctcgagcgc
     2161 ctctgccgcg gatttcgtcg acgtcacgcg cgcgccgtag cgcctcagca ttgtcccgag
     2221 cagtttccgt gagtcgtcgt catcctcaac cagcaacacg tgcacaccat caagccgcac
     2281 cacgcctttg cgactgtgcc gccggtcgac ttcacgcttg cgctcttctg ctcgcgccgg
     2341 atcgcgtcgc tcctgcgtca tcggcagatc gaccgtaaac accgatcccc tgccgagtcc
     2401 gtcactctcg gccttgatcg ttccaccatg cagctcaacc agatgccgcg cgaccgccag
     2461 acccaatccg agcccgccct gcttccgcga gatcgatcct tccgcctgcc ggaaacgatc
     2521 gaacacgtac ggcagaaact ccgctgagat gccgtcgcca gtatcactga ctttcagacg
     2581 cgccgtcgct tcaacacact tgagatagat gctcacgctg ccgccgcggg tcgtgaactt
     2641 gatcgcgttc gacagcaaat tccagatcac ctgccgcaga cgatttgcat cgcctttgac
     2701 caggcactca ctcggctcga agcgcgtctg gagttttatg cctttcacgt ccgctgtcgg
     2761 tcgcaacgca tcgagcgcgg cttcaacaat cggcccgaga ttcagcgggt gtaaatcgag
     2821 acgtagatta ccagtgatga ttctcgacac gtcgagcaga tcgtcgatga gtcttgcctg
     2881 cgaacgtgcg ttgcgctcga tcgtctcgat cgcgcgtgag gcgttggagc cttcaacttc
     2941 gccgttccga atcatcgatg cccaaccgag aatcgacgtt agcggcgtgc gcagctcgtg
     3001 cgagagcgtg gctagaaact catctttcaa ccgatcggct tcttcggcgt gcttgcgcgc
     3061 atcctgctcg cgcgacagcg ccgccgatct tgcatcttcg gcgctctgtc gcgcagtcac
     3121 gtcgagcacc agtgacaaaa ccgagatcag tttgccgcga tcgtcgtgca gcacggagtt
     3181 gtaccactcg caaaagagca cggcgccgcc gcgtgtatag ttgcgattgc gcaggatgcc
     3241 gtgaacttca acgccttcgc gctgccggtt cgtcacgagt gcgacggcgt cttcatcgtc
     3301 tgcaaaaacg aagcgccact cgttgacgtg tttcccgatc acctcttccg ctttccaacc
     3361 gaagagccgt tcagccgacg cagaccagcg cgagacgcga aagtcgctgt cccactcgac
     3421 gacggcgagc ggcgagctct ccacgtgaaa tgcgagttgt ttatatgcgg tctccgccgc
     3481 tccttcggca tgcttgcgtg cggtgacgtc agtgattaca gcgatgccgc cgccgatctt
     3541 gttttgctcg tcgagaagcg gtgagtaatg actctcgaaa acgccggtct cggccggtcc
     3601 gtaagggtgg tactcggaaa ctgactcctt accttcgagg gccgcgagga aacaactgtc
     3661 gttaccttct tctcgcaaaa aaggaaagat gtcgccgacg cgtttgccaa gcacagcttc
     3721 gcgactgaaa cccgagatgc gctgcatggc gagattccat gcagtgatcc ggcagtcgcg
     3781 atcgaacgcg aagatgccgt cgacgctcgc gtcgatcagc ctgttaagca gaagtgccga
     3841 agtgttttcc aaagttagag ccacgggatg gggatcgatc ggtggtctac tttccagccg
     3901 tgagcagcat taaactggtc gcgtcgcgca ccagtatatt agatccgagc agtaccggca
     3961 tggcgaaggt cgaggcttca gccacttcat aacgacgctc gaccgtgaag gccgcggtac
     4021 cgcgtttcgc aacgatcaaa tcagcgccgt tcgtcaggaa aacgacgtgt tgcggagtaa
     4081 gtagtaacga agcgtgctcg ccttccctgc cctcagtcgt ccagcgcacg gcgcccgtct
     4141 tcgcatcgat cgcaaaaaac tgtcccttcc tctttgatga atggccgtag atgaggccgt
     4201 cgccgaagac tggggagctc atgtacatcg cgatgtcagg gttcttccag gcctcggttg
     4261 cctgccactt gccggcattc tgcgcgaggg tataggcgtg tgtgccttgt cgcgtgcccg
     4321 agacgaccag gtgtgtgccg gtccagagtg gtgtggttat gttctcatgc cactcgtctg
     4381 gaaatgtgac cgaccacagc tctttgcctg tcttgccgtc gagaccgacg atcgagcctt
     4441 cggtcatcgt cacgatctgc tggctgccgc cggcttcgat catgactggc gaagcgtagc
     4501 ctggtccaag tcccttccat tcccattcgg ttgctccagt cgccgggttg agagcaagaa
     4561 cctgaccgcc atgaatgtca ctgccaacct ggaccacgac gcgtccgttg acgagcaatg
     4621 gcgacgctgc ggtaccgcag aacaacttcg aagtgtcgat tgcgtttgaa aaatctttgg
     4681 accagagttg ttttccggtg gtggtgtccc aggcattgag aatgccggtg acgccgagtg
     4741 tgaacagtcg attaccggcg atgagcgggg tcgcatttgg tcccttcgcc atactgacag
     4801 cgtactggtt cttcttgaac tcagcctggt atttctgctc ccagaggact ttgccatctg
     4861 cgaggttgat gctcgccaca atctcttccg gatcgcgtcg tccatgaaca aacgcgcggc
     4921 ccccggacac gaccggcgac gagtaaccct cgccaatctc gacgcgccac gtccttttca
     4981 gtgactccgg ccacgccgcc ggggcatttt tagtcgtgac cactccatcg cgtgccggac
     5041 cccgccactg cgtccattcc tgcgccgtta cggtgactgt agccgtcaga aatacaaaaa
     5101 ttaaacctag cttgttcatg ggttctacct cttggtcagg acgaggatca tgccacaaaa
     5161 aggcacaaaa agcagtccac agattacaca gattgcggag attttttata actatctgtg
     5221 taatctgcgc aatctttgtg gctagacgac gtcggcgaag ctcttgcgtg tgcgggcgaa
     5281 ccaccagtag ccgaagagga agaggaggag ggcaaaggtg gtgaagtagg cgaggccgcg
     5341 ccagtcgggg gtggtgccgt cgagaaagat gtggcgatag ctgcgaatca gcgaagtgaa
     5401 cgggttgagg ttgatgaagg tgcggaaacg ttctgggacg atcgcttcag ggtagataat
     5461 gggtgtcaga tacatccacg ccatcagcaa aagcatgatg ccttgggtga tgtcgcgcag
     5521 aaagacgccg agcgacgcga cgagccaggc ggcgccgagt gcgaagagta gttgtggaat
     5581 tagcagtacc ggaagaaaga ggctggtcaa ctcgagtcgc tggcgaatga tgatcgtggc
     5641 gatcagcaaa gcgatggtgg cgaagagttg actgccgagt gccgcaaaga cctgtgcagc
     5701 gggcagtgct tccaacggga acactacgcg cttcacgaga ttcgagtggg ccagaatcgt
     5761 gttcgcggat tgctgcacgg attcctgaaa catggaccac ggaagcagac cgcagaagag
     5821 atagagcgcg taatcccagt gggaatcgtt ggcgccgaaa cgggcgccga agatgcccgc
     5881 aaagatgaac gtgaagatcg cgatcatcac gacgggcgtc agtacggccc aggcgatgcc
     5941 gagcgccgag cctttgtaac gacccagcag ctcgcgcttc gcgaacgaga agatcagatc
     6001 cgcgcgcgac gggagttgcc agagtggctg ccagatgtgg tttgctgtag acatcctgtg
     6061 aattttgtaa atcctgacat catgttagac aggattacag gatttacaag attaacagga
     6121 tatttttccg agtccgcgcc agctgttctt gttatgccag ctaatctcga cgtcgcgagc
     6181 gtcgatgtcg cgccaccacg tttcgaagtc gtcgcgcgag atgtaaaacg cagtgggcgc
     6241 gacgagatga tcgaagacga tcgtgtgctg ctcgcgccaa ccaaagtttg agatcgcgct
     6301 cagatagtcg ttgtagaaaa gccgtttcgc gagcgaccgc ccgcgcctgt tcaatggtcc
     6361 atacacgagt ttcgtcgcca ggtacacacc cgcggcgggt aacttcgata gttgcagcag
     6421 cgcacgctga tcgatgcgcg aagtgatctt ctcgcgcacg ggattcacga aacgcgtgat
     6481 ccactcgttg ttctccgcgc cgtaaatcca cgccgagagg tgtccaccgg gcttcacctt
     6541 cccagccaaa gccaggaacc cgccacgcgg atcgggcaag tgatgcaaca cgccgacgga
     6601 aaaagcatag tcgaacttgc gcgcgagcgg caggtgatag atgtcggcct ggatgatgtg
     6661 tgcgttgggc aacgagcgtg tggccgcgaa ggctgtctca actgcgacgc tcagatcgat
     6721 gccaatcaca tcgcgtgctc cccaacgcgc ggcgagttgt gtatgacggc ctttgccgca
     6781 tccgccttcc aacacgatct tgtcgcggaa aaattcgggc gtaacgggcg cgatccagcc
     6841 gagaaactgt tcggcgtagc gttcgtcttc ctgtgtgaag tgttgccact gccagccgaa
     6901 acccgcggcc gtggcgcgct tgtcgctctc gacgtgatcg agatttgcga aacggggaat
     6961 gccggcagtg atgggaaatg aaatcgagca ggacgtgcaa cgtagctcgc cctcgatgat
     7021 ctcgccgtct tcggttttag tgacggaaag atcgagcggt acttcacacg aggggcagat
     7081 tagataacta agtaggcttt gcttcaagcg aggtcaatat aacatttagc cacggattac
     7141 cacggataca cacggatcag aaaattattc tgcagtgaaa atccgtgtta atccgtggct
     7201 aattacattc gcgactattg tgagtcgggt tcattgtgaa gcggctttga tattcggcgg
     7261 aggtcaggaa gttgcaaacc aaagaacgcg cggcgtcggg gtcgcggagg ggtttggttt
     7321 tgagtgtgtt gagccaggtg ttgtatccag cctcgtcagg attgcgacgc aggtaagtga
     7381 aatactggaa cagcacgagc gcgtggttgt agttcgcatc gacaacgcgc tggtccgaag
     7441 cgagccgcgt caaaactgcg gcacgaccat tcgcactatc gtccagcaaa ccgatcaata
     7501 aactacgctc cgaggtgaga tcgacgccgg tagtttgagc catcgtcgtg agcagcgaat
     7561 cgacaaactc agacggcttc atcgtcgcgg gaaatcgccg cttaaactca gcacgttcca
     7621 cgaaagcatt cacgagcgcg aggcgcacgg attcaagctc gctcttctga ttcaaaacca
     7681 gcgcgcgatc ggtttccagc tccgcgtaac gcggctggcg accgagtccg gtcgagtaaa
     7741 gcccgtagag aaacacgccc gtcgccggta attcattttc aatcagcagg ttcgtcgaga
     7801 tgtcgattcg gcgtgtgcgc aggcagtcgc tgcgtaaatt gcagccggta agttgcgcgg
     7861 tgagtttgtc gagcgtggct gaatcaggct cgcgaccagt cgagtctgtg tactgctgcg
     7921 tgacgaagaa gcggaaatca tcaaccggat ttgagagcat cgacgcgaca cccggatcga
     7981 tcgtgatcgc gcccggcacg tatgcagtct caccggagtt ggactggagt cgcagcgtgt
     8041 aatcgccaaa cggcgcattc ggcgctacct gaacttcgat actgacaatc ggaaacggcg
     8101 cggcgatctg ctcgcgcgtc aacgttgccg gatccacggt gaagaacggc gagttgacgg
     8161 cgatgctcgt gccgggcacc tgatcgacac cttctccacc gaagtaaatc ttcacccgct
     8221 tgcccggcgt gagcgggagc gccacagtcg agagctcagc gttgagccca atcattttcg
     8281 gattcaggac cgaagcctgt ggctgtacga ggctcgaatt cagcggagtc gccgcatcag
     8341 ccttgacgac aacctggctc gacaactcga aactgcgaaa cttctgcgct gcatcgacac
     8401 ttgacgcgac gaccactcga tactgccccg gcgtaagtcc ttcgagacga taagtgccgt
     8461 cttcagcagt cacatcactc gcgatcagcc gtccattaac aacactttcg gcccacacgc
     8521 tcacaccgtt cagcggcgtc aacgcaccgg cggtgcggtt atcgacgagt cgtccttcga
     8581 tacgcccgag cttcagattt gaaccgtaga gaccgcgaat cttctgtcga tcttcttcgc
     8641 tcagcgtgcg ctcggtcagc gccggcaagc cgaaagtgcc gttgaacgcc tgccggccct
     8701 gcatcgtcga cgacaaaacg gacgaatggt ccaacccgag caggtgaccg atttcgtgtg
     8761 tgaacgtcgc ttcgagatcg tacgtaccgg gcgtgccgtc agtggaaaac tgaagctcgg
     8821 ttccgtcttc ggcgcgcggt cgtggattga tgctgatgtc tgcttcggcg atgttgccgg
     8881 tttcgggatc gaagaaaacg cgcgtgcggc cggtggtcgc ttcggagttg aatgcttcgt
     8941 tttcgggagt gacggcgatc gtcaaaaggc tgataccgtc acctgcttct gcgggactga
     9001 cagacgctgc cggagaccac gtcacgataa agttgatatt cgagagactg gcccagcgag
     9061 cgagtgcacg ccgtgcggcg ccaacgacat cgctatccgg tttgatgttc gagcccggcg
     9121 agagcagaga cgttgagagc gcgacctcga tggtcttctt cggccagcgt attttgcgaa
     9181 cagtttgaat ggaaggttcg aactcgaacg agtcgcggat ctcgagccgc tctctggtac
     9241 cgagagaaaa aaatagtgcg agactcgtaa tgagtgcgac ccgatactgc atctgaactt
     9301 ccctggcggc gaggtcgaga aacgcgcccc gcatcgtacc acggttggcc accaaacggc
     9361 acaaaaagct ttagccacaa aaggcacaaa caagaacaca aaaggcacaa atcagaaaaa
     9421 gcctattagt gagggatctc gctcaattta tcgagcgact cgccatcagc taataggctt
     9481 tgtgcctttt tgcgttcttg tttgtgcctt ttgtggctga tcttaattga tcgttatgtg
     9541 tggcgcagtg tccgcaggcc ggctgactgc cgtttgctgg gtgccccgcg taaacgtcac
     9601 ctgtatcgga acgtctccgg cgcccgctaa cgaggccgga agggtgaacg taatgatgtc
     9661 aaacccgggc atttcccggt ttggctgaac ggaaaggatc gcgtcgccgg tgatgttcgt
     9721 tgttcccacc atgacgctga cctcagtccg cgcggcaaag cgcacgcccg taacagtcaa
     9781 ttcgagcttt gtcggtacct gagtccccgt gccgtcgtca gacgtgacac tgaacggctc
     9841 agccaggcgg ttcatcggat tcgtgatatt gatggcggcc gcccgcccac cagcatccgc
     9901 tgtagtagtg aagatatcag gctgagcgaa aagaatctgc acgagcccgc gctgaagcgt
     9961 atcggtattc gcgccggcat cgagcacatt tactgcgacg gtgcccagcc cggacgctaa
    10021 accaatcggc accacgaaat tgatctgctt gctggtgttg ccgacgaaat agagccctgc
    10081 cgcggcgccg tttacgctga ccgaaacacc gttaagctcc actggcaatg ccggactgcg
    10141 cgtcgtctcc gacgcccccg atgactgtag atcagacgga gcgagcacga ctgtcgacct
    10201 aacaatggcg agttcgcccg gtgcgagacc agccggagtt ccgggaaccg ggctcggcgt
    10261 tggtgtcgga ctcggagtcg ggctcggaac cggtgtcgca gccgcgacag gcatgttgct
    10321 cgcacccgta aagaatgaaa gcacggccga tgactggctc gtgatggtcg gtgttatcag
    10381 gtagaaaact tcagtcgaac catcgccatt gccgccacca atctcggcgc cgccaagcga
    10441 aaacgtcatg cgcttgcgcg tttcactcgc gagtggtcgc gtgccgccaa acgtctggag
    10501 attcgtgatg ttagtgattc gagtaaaggt gttcgtggag gtaatcggaa gcggcgttat
    10561 gtaaatctcg gccgagttgt cggtgttcaa accttcaggc gcggtcgcag tggaatccgg
    10621 aaacgttccg ttgggacgga agttcagaaa cgaagtgaac acgagtgtcg acgggtttcc
    10681 cgatgcgtcg taatcggtga aagtcggaaa gcgcgcgaag tctgaaatcg gcggtctggt
    10741 gccgacctca ttgaacgtat cagtcccgac ggtataaacc caatttccca aaacgctgtt
    10801 cgccgccggc gcggcatttg attttggatc ggtcgcgcgc gattcgaagg cgatcaggcc
    10861 gccgttgcga ctcatcctgc gacccggact caacacattt gtgttcgcga cgttgttcaa
    10921 cgtccgcgtg acctggaaga aagcgccgac agtgccaccc gaaaacgtgg tcgtaaaaat
    10981 ctcagcgttg ttgtcggcgt tattagtggc caggttggcg ctcgacatga acgccacctt
    11041 cgacccgtca gaagacagca tgggattggt agaaaagatc agaccaactc cagcgacggc
    11101 gtcctgggtg tgtgtcccct gggtaaatgt tttggttgca accgtgtaaa agaacagttc
    11161 gggattgaaa tcagcattac caacagcggg aacgagattt ctcgtggaga taaacgcaat
    11221 gatgccgcca tcgtcagaga tcgtcggctc gcgattatcg tcggcgaaaa atggcgagtt
    11281 gctggtggat cccggcgtcg gtgctcgact ggcaggcgtg ttccccgtaa ctggatcaaa
    11341 cgtccccgtc gaaagatcct gaaatggcag atcggcgccg agagttagat cgacgtcggc
    11401 aactgccgga acacgataaa tccagatctc cgaattcgcg tcagcgtcga gagtgtcgct
    11461 atcagatcca tcgaagaact ttggatccgg cgcatttgag ctgaagacaa tgatgtaact
    11521 gcgcacgccg ccgaccagtg tcggcgccag ggagatcatc ggcgtccgat tgtcgatctc
    11581 gaccttcacc tttgaccggt cagccggcgt aggttgtggc gtcggagttg gcgaaggtga
    11641 tgcagtaggg ctcggcgtcg gtgttggact cggcgtgggc gtcgggcttg gtgacggtgt
    11701 aacgaccgga acgttcttgg tgttggtgat ctggaagatg cgacgttgcg cgtagtcggc
    11761 cagaaagatc tcgcgattgc cgtcgacgtt gttcggattt acagtggcaa cgttaccatt
    11821 cgaagtgaaa acgacgaagc ggccgtttgc actgatgtcg ctcacgatcg agttaaaagc
    11881 tgatgtcgga gttgaagtga gctgaacggt gaacgggtcc ggagtcggac ttgttgattg
    11941 ggccgatact gaggcaagcg agccacctaa agccacaggc acgatgatgg cgcacaagaa
    12001 ccagaaaact tgcttcacag attcctccga aataacaata aagacgcaga tacgagcaga
    12061 ttagatgccc tcggggagct gagccggcga ttaaagactg gccattttaa gcagaacccc
    12121 tccgcttcgc aagcagggat ggtttttgat aatgtccttt tccgcagtat aggtcctatc
    12181 ggccctgtac gacctattcc accggagaaa aagaatgccg aagaaagcgc tgattactgg
    12241 aattaccggt caggatggca gttatctcgc cgattacctg ctgagtttgg gttatgaagt
    12301 acacggattg gtccgccgcg tggccctcga agccccggag acgaggttca gccgcatcaa
    12361 gcaccttttg gatcgagtta cgctgcatcc ggccagtctc gaaagctacg cgagtatttt
    12421 ccacgtgttc agcaaacacg attttgacga gtgctatcac ctagcggcgc agagtttcgt
    12481 ggcggagagt ttcgctgacg gcttttggac catgagcaca aacatcaacg gcacacatta
    12541 cgtgcttgcc gcgttgcgcg agctgcgacc gaagtgtcgt ttttacttcg ccggctcgag
    12601 cgagatgttt gggaacgtca acgaagtgcc gcaaaaagag acgaccgcgt ttcaaccgcg
    12661 cagcccttat ggaatcagta aagttgccgg tcaccattta accaggaact atcgcgaggc
    12721 gtacggactc ttctgcgtca gtggcattct cttcaatcac gagagtccgc ggcgcggatt
    12781 tgagtttgtg acgcgcaaga cgaccagcac cgtggcgcgg atcaaacacg gtttagccaa
    12841 cgagctgcgt atcggaaatc tcgaagcgaa acgcgactgg ggacacgcga aagattacgt
    12901 gcgcgcgatg cacgcgatgc tccagcattc ggaaccagac gattttgtaa tcgcgactgg
    12961 agagacacac agcgtgcgcg agctttgtga gatcgcgttc ggcgaagtgg gtctcgatta
    13021 tcgtgagttt gtaaaagtcg atgagagata ttaccggcct gcggaggtcg atctgctgat
    13081 tggcgacccg tcgaaagcgc gggaagcgat cggatgggag ccgacgtaca cgttcggtga
    13141 gttggtcaaa gagatggtgg cgtcggattt gaaagcgcta aatgaagagt tccctaaatg
    13201 tccgacaaac ttcagtttgt cgcgcaatcc gacaagctaa agcatgtcgg acatcaaact
    13261 tttttaacta tgcccattgc tgttcgcgtc gattccgtct cgaaacaata tcgcatctac
    13321 gatcggcctg cggaccgttt gaaagagaca ttgacgcgtg ggcattggaa agcgcatcgc
    13381 gagttctggg cattgaaaga cgtcagcttc gaagttgaag caggcaccac caccggcatc
    13441 attggtccaa acggctccgg caaatcgacg ctcctgcaga tcattaccgg cacactcgaa
    13501 cccacacacg gaagcgtatc gattgaaggc cgtgtcgcgg cgttgctcga gctcggcgcg
    13561 ggattcaatc cggagttcac cggcatcgag aacatcttca tgaacgcctc gttgatgggt
    13621 ttctcgaaag ccgaaaccga gcaactgctt cccgagatcg caaacttcgc tgagatcggt
    13681 gacttcattt accagccact gaagatgtat tcgagcggca tgtacatccg tctcgctttt
    13741 gccgccgcga tcgccgtcgc cccgcagata ctgatcatcg acgaagcact tgcagtcggc
    13801 gatgcggtgt ttcagcatcg ttgtacgcgc cggattaaag agatgcagga gaatggcaca
    13861 acgattctgt tcgtgtcgca cgatccgtcg gcggtgcggg cgctctgcaa tcgcgctgtg
    13921 ctgttgaacg gtggacagaa gatcgctgaa ggcacacctg ctgacgtgct caatcgctat
    13981 caaaagatca tcatggcgcg gcagcaggca tacgaagcga gctcgactaa gaccgcggac
    14041 cagactggtg aatttgttga acgcaagaca acgccgctga gttacgttta tcgtcatggc
    14101 gatcgcagcg cggaagtgtt ggaagtcgag gtgctcgaca actcgttaca tccggtcgag
    14161 ctggtcgaga ctggcgagcc ggtcacagtt cgcattgttt atgtggccca cgaagatctc
    14221 gacgatgttg tgtgcgggtt tctgatcagg aatcgtcatg gcattcacat ctacggaacg
    14281 aataccgagc ttcaggaagt gccgtttgaa cgagtgaggc gcggcgagtt aaccgaagtg
    14341 acgtttgagt tcaactgctg gctcgcgccc gagatgtatt cgctgtgcgt cgccgtgcac
    14401 agcagcgcgg ccgtgagctt tgattggctg gacggctgct tgttctttag agtgatgtcg
    14461 gcaacgaaga tggaaggcgt ggtgaatctg aacgcgacgg cgacgaccaa gcgcctcgca
    14521 aggtcatcca cagattacac agattcagta aaacaatctg tgtaatctgt ggataaatac
    14581 gatcgaaatg gactcgcaaa tagaaactga cggcctggac ctcgcgcaac tgatggccca
    14641 gatacgcaaa gacgccgaga ctcgtaaacg cagctcgctc aaccacggcg accccgcgcg
    14701 ctatcgacag ttaatcgttc aaaacttcaa cgccttcctc gcgccgcaaa tctcctcgct
    14761 gccgcctctg aaactgcagc cggagctcga aaaacgcgat cgatatcacg tgaacgatct
    14821 gctcagtttt cacgatgaag tgtttgtgcg gaacgcttac aaaatcatcc tgaaacgcga
    14881 acccgacgac tcgggcatgg cccacttcct caaaaacctg cgcaacggtc gttacagcaa
    14941 gcttgatgtt ctcagcgcgc tgcggttttc atccgagggc gaaaacgtaa acgtacaaat
    15001 cgaaggtctc agccggcgtt cggtgctgcg gaaaatgtat cgcgtgccgg tggccggtta
    15061 tctgctgcgt ctggcagtag caatcgcacg actgcccgtg ctcatcacga acttccgccg
    15121 gctcgaatca cactcgatgg cccagctcga gcgcgtcgcc gctcacgtca acgacgccgt
    15181 cgcgcacctc tcgcaagcaa accgcactca cttcgaatcg ctgcaaaaac aactcaacga
    15241 tctgaaagga actgtacagc tccagatcaa ctcgctcgtc cgcgagcagg aaaaattctc
    15301 gcaggtccag actgtgacac ggcaaaatct cagcaccacg cagtcagaac tttccgagcg
    15361 catagatctg acgacgcatc atgcacgcga acaagacgag ctttacgcgg ctgagttgca
    15421 ggcgttgcga gtgcaggttg aaaagcagat cgacgatctg atcgagcggt tgcaaaggtc
    15481 gcggatggac ctcgcgcaac aggaaacacg tctctcgcgt ctgctcgaag gcgccgtttt
    15541 aacgccgtca actccggctc agccatggca cccggaacaa gatcacctgc tcgattcgct
    15601 ttacttctca ctcgaagacg ttttgcgcgg cacaaccgaa cagatcaaag aggaagtgaa
    15661 aatttacctg ccggtgttgg gaaacgccgg aatcagctct gagatactcg acgtcggctg
    15721 cgggcgcggt gagtggctcc aggtgctgcg cgaggcggga ttgcaggcgc gcggaatcga
    15781 tactaatcgc attctggtcc aacagtgcaa agagttgtcg ctggacgttg aggaacgaga
    15841 ggcgctggag tttctcggct cgctgccgga tgggagtctg aatgcggtca cggcttttca
    15901 cttcgccgag cacctgccac tcgaaacgct cgtgcggttc ctggacgaaa ccggacgcac
    15961 gctgaaaccg ggcgggctca taattctcga aactccaaac ccggaaaatc ttttggtcgg
    16021 cagttgtaat ttttatctgg acccgacgca taagaatccg ataccgattc cgacgatgaa
    16081 gctactgttg gaagcgcgcg gctttcgctg cgaagaagtt ttgaaactcc atgcagtttc
    16141 gtcgacgaag atcgaagtca aagatcaact gaccagccat ttaaatcatt ttctgtacgg
    16201 tccaatgaac tacgccgtcg tcgcccgcaa acccgaatcc gaatgaa
//
LOCUS       DASSFQ010000278         9163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2077012, whole genome shotgun sequence.
ACCESSION   DASSFQ010000278 DASSFQ010000000
VERSION     DASSFQ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077012"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="VFH96_10090"
     CDS             complement(<1..1540)
                     /locus_tag="VFH96_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974364.1"
                     /translation="MDSLIKDIRYAIRNLIKRPGFVAIAVITLALGIGANTAIFSLVN
                     TVVLRSLPVDRPNEIVSVAIRGKNDSMSAFSYPNYKDFRDHNDVLSGLLVYRFVPLSL
                     SRGGNNERVWGYEVSGNYFDVLNVKAIHGRTFLPEEDQTKLSHPVIVVGYECWQKRFG
                     GDPSLVGKDVLINNHQFRVIGIAPEGFKGTEFVYSAEIFIPASMMEWVEPGATWIDNR
                     NSKNFFAIGRLKSGVDWRQAEASLNLLGQQIAKEYPDSNEGQSIKLDAPGFILPDLRG
                     AVVNFTWVLMAAVGLVLLVTCTNLAGLMLARATDRRKEIAIRLAMGANRLRLIRQLLT
                     ESVLLASAGGAVGVLLAIWILRGLLALKPPIDFPLMLDVSVDWRVLLFSLAVSVTAGV
                     IFGFAPALQATRPNLVGALKDTAAQGGAVKTKLRSVLVVAQIAISLIVLISAGLVVRT
                     LQRLQTMNPGFDTKNAITMSFDLGLQGYDEARGQQFYKQLQERLQTLPGVESAAVMSY
                     IPLSL"
     gene            complement(1571..4072)
                     /locus_tag="VFH96_10095"
     CDS             complement(1571..4072)
                     /locus_tag="VFH96_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974365.1"
                     /translation="MESMIKDIRFAARGLLKRPGFTVIAVLTLALGIGANTAIFSVVN
                     ATLMRTPPVSDPERLVFVFNGPAGNVFSYPDYAAVRDQNNVLDGLIAWGGITTSLNSN
                     DQTDLVTGAIVTGNFFDVLGVRAQMGRVITTEDDKTPGAHPVAVISQGLWQGRFAGDP
                     NIIGRQLLLNGNTFTVIGVLPKGFDGLQLGITRDLYVPMMMQATMRPPRAGFSGEMNP
                     DLLQVRTNRWLFSVGRLKPRVSEAQAQSSLALITKQLEVAYPQQSSGRGITISPLSAS
                     DDPDGRQQLSSVARLLMGVVGIVLLIACANVANLLLARSSARTKEIAVRLAIGATRWR
                     IVRQLLTEGVLLATLGGAAGLLLAWWTARTISAATPPTGALPITPQFSIDLRVLLFTL
                     GLSLAAGVIFGLAPALRSSKPALIPALKDDSTAFFERTRIFTLRNLLVVTQVALSVVL
                     LIAAGLFLRSLRQAQTIDPGFDAEKIVGLPLNINLLRYTKAQGREFYRQVTERVEAIP
                     GVESASVARIAAVSGNSSVRSLMIEGRAGSENQFRSDNSGPVAENNESVSANVIGPKY
                     FQTMGIALLQGRDFNAQDSDDHPPVVIVNEAFVRRHFPNEDALGKRLSFNGPKGPWRE
                     IVGVVRTSKYRSLGEAPTPVAYVALQQNHETGMVLHVRTRVDPSSVAGAIRNEVQSLE
                     KNLPLGNPELMSERVANSLYAARMGAILLAVFGGLALLLASIGLYGVMSFAVARRTRE
                     LGIRVALGAQPGDVFRLVLLQGMTLVIAGLLFGIGVALAVTRLLASFLYGVSTMDALT
                     FTAIPALLTFVALLACYAPARRATKVEPLVALRYE"
     gene            complement(4143..6605)
                     /locus_tag="VFH96_10100"
     CDS             complement(4143..6605)
                     /locus_tag="VFH96_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974366.1"
                     /translation="MENLIKDIRYGVRMLMKNPGVTIVAVITLALGIGANTAIFSGVN
                     AFLMRPLSVPNAAELVRPLEISDDRGTTDEFSYPDFLEYREQSSSFSGIAAEDMIQAA
                     VDTENQNDVVWGQVVSANYFDVLQVKPILGRAFLPDEDKTAGASHVVVLSHSFWQRRM
                     AGDPNIVGKTVQLNNRAYEVIGVAPDYFVGTKFALALDFWTPISMAEELRRNPGILAD
                     RGSHWMNAIGRLKPGVSVEQASAEVATIAARLNQSYPNERAATTQGQVLGEIDGRWAE
                     MGPVFKSASAIAMAIVGLILLIACANVANLMLARAAGRRKEIGIRLALGANRARLVRQ
                     LLTESMLLSIAGGGLGLLLAVWVTDLMEGFIPILEYNVVSNFFALDSRALLFTLVVSL
                     GTGLIFGLAPAWHSSNPELVPVLKGETEKARRGKRRGLGLRNVLVVAQVALSLVVLVC
                     GGLFIKSFRKAQTMDPGFDNAKGLIVSLSPTLIGYEDEQSRQFYKQVLERVSHVPGVD
                     AVSYARTLPLGDSSNSNGPVLKEGETLAKGSAGRVIMTTVISPGYFKTMQIPILDGRD
                     FDDRDQSKTQRVVIVNQEMAEMLWPGESAVGKRIFIGVDSKDAWEVVGVVKTGKYRTL
                     AEDPKPFFYYSMGQRRPAPTAMVVRAGVDPRSLVGAIRSEVQAIDRRVPLSGVKTMDE
                     HKTYALWAPNMAASFSVAFAVVAILLSAVGLYSVMAYVVSQRTREVGIRMALGANRSD
                     VMKMITRQGMRLAAFGVVIGLLLSLALAQVLSSLLIGVSGYDVATFVLVPGVLSVVAL
                     VACYLPARRATKVDPLVALRYE"
     gene            complement(6651..7721)
                     /locus_tag="VFH96_10105"
     CDS             complement(6651..7721)
                     /locus_tag="VFH96_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974367.1"
                     /translation="MKLLNHSLKARILWLLASFIAIVIASPLPQLYAQTSRDQTQSVT
                     RWEYTDTDVGLKRRFETRGKLEFTDDYTDIKSVSDGGWVIIEEHRDSQSYRYEVRRDA
                     SGQLQRLFYVNGAAKPIDASAKAWLAKFVLDAVRQGAFDAEKRVKTILGQRGVTGVLA
                     EIDQIQGDWARRIYYEQLLKQANLDGGALRDVLRQMARNISSDYEQAQLLTAVAPMLT
                     GREAALQPFFDAVATIKSDYERGRVLKAIMKQETNSSAVLVLVASSTKTISSDYERRG
                     VLSALVKARNQSEEVLRLLLDSAAAMSSDYEKATLLMEVSNAYTGDTRLKGLFLKAVE
                     TIKSDYERGRVLSALLKNKQIG"
     gene            complement(7725..8264)
                     /locus_tag="VFH96_10110"
     CDS             complement(7725..8264)
                     /locus_tag="VFH96_10110"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6974368.1"
                     /translation="MNERQSQYAWHFDPAYTTVEFVIRNLWFKVRGRFRDLEGVIVLD
                     ENDVSRSSVTATIRPDSIDTGNKRRDGHLLLRDFFDVEKFPEIEFRSTNVRRGKDRDS
                     LDLEGELTIKDKTIPIALAVNEMDRSRSPSGEEFVYYSATTEIDRCAFGINYGRGLIG
                     RRLRVHINVQASNTPPKGK"
     gene            complement(8309..>9163)
                     /locus_tag="VFH96_10115"
     CDS             complement(8309..>9163)
                     /locus_tag="VFH96_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6974369.1"
                     /translation="PDRELHTGVNVTDAGYFQTMQIPLKLGRFYTDQEVAEMRHVVLV
                     NETFVQKNLGGQNPLGHKLTIYMKDDNVPSEIIGVVADHRHLGLDVAIEPMVYWPHVE
                     LVYPGMTLMLRTRGDANAVAGAARNVIHTIDPQQPIGEVNTMATLLSTSVARARFSAS
                     LLTVFSFVALIMAAVGIYGVMSYSVLQRTHEIGVRMALGAQRFDVLKLVVRKGIVLGV
                     IGVVVGLIASFALTRLISTLLFEVTATDTATFVGVSFGLFFVTLAACYLPARRATRVN
                     PLKALRYE"
BASE COUNT         2043 a         2785 c         2472 g         1863 t
ORIGIN      
        1 tgagactaag cgggatataa ctcatcaccg ccgctgattc gactccgggc agcgtctgta
       61 gtcgctcctg taattgcttg taaaactgct gaccgcgcgc ttcgtcgtaa ccctgcaagc
      121 cgagatcgaa cgacatcgtg atcgcgtttt tggtgtcgaa gccggggttc atcgtttgca
      181 gtcgctgcaa agtgcgcacg acgagtccgg ctgaaatcaa aacgatcaac gagatcgcga
      241 tctgcgcaac gacgagtacg ctgcgcagtt tcgtcttcac ggcgcctccc tgcgcggcag
      301 tgtctttcaa agcgccgacg agattcgggc gcgtagcctg caacgccgga gcaaagccaa
      361 agatcacacc cgccgtgact gacaccgcga gtgaaaaaag caacacgcgc caatcgacac
      421 tgacatcgag catgagcgga aaatcgatcg gaggcttgag ggccagtaac ccccgcagga
      481 tccaaattgc aagcaacact ccgacagcgc cacccgcaga cgcaagcaga acgctttcag
      541 taagaagctg tcggatcaaa cgcaggcggt tggcgcccat tgcgagacgg atcgcgatct
      601 ctttgcggcg atcagtcgca cgcgcaagca tgagtcccgc cagattcgtg cacgtcacga
      661 gcagcaccaa tccaaccgcc gccatcaaca cccaggtgaa gttaacaacc gcaccgcgca
      721 ggtctggcag gataaatccc ggtgcgtcga gtttgatcga ttgtccttcg ttcgagtcgg
      781 gatactcttt tgcgatctgc tggcccagca ggttgagtga agcttctgcc tggcgccaat
      841 caactccgct cttcagacgt ccaatcgcga agaaattttt cgagttgcga ttgtcgatcc
      901 aggtcgcgcc gggttcgacc cattccatca tcgaggccgg tataaagatt tccgccgagt
      961 agacaaactc ggttcctttg aaaccttcgg gcgcgatgcc gatcactctg aactggtgat
     1021 tgttgatgag cacgtctttg ccgacgagac tcggatcgcc accgaagcgt ttctgccagc
     1081 attcataacc gacgacgatc acgggatgcg agagttttgt ctggtcttct tcgggaagaa
     1141 aggtgcggcc gtgaatggct ttcacgttga gcacgtcgaa gtagttgccg gagacttcgt
     1201 agccccagac gcgctcgtta ttgccgccac gactcaggct cagcggcacg aagcgataaa
     1261 cgagtaaacc cgagagcacg tcgttgtgat cgcgaaagtc tttgtagttg ggataggaga
     1321 aagccgacat cgaatcgttc ttgccgcgga tggcgacgga gacgatctcg ttgggccgat
     1381 cgaccggcag agagcgcaac actaccgtgt tcacgagact gaagatggcg gtattggcgc
     1441 caatgccaag cgcgagcgtg atcaccgcga tcgcgacaaa ccccggcctc ttgattaaat
     1501 tccgaattgc atatcgaata tctttaatta gtgaatccat aatccgtttc ctatccgcgt
     1561 taatccgcgt tcactcatac cgaagagcca cgagcggctc gaccttggtc gctctgcgag
     1621 ccggcgcgta gcacgcaagc aacgcaacaa aagtcagcag cgccggaatc gccgtaaacg
     1681 tcaatgcatc catcgtgctc acaccataaa gaaaactcgc gagcagtcgc gtcaccgcca
     1741 gcgccacacc aataccgaac aaaagcccgg cgatcacgag cgtcattccc tgcagcaaca
     1801 cgaggcggaa cacgtcgccg ggttgcgcgc cgagcgcgac acgaatcccg agctcgcgcg
     1861 tgcgtctcgc aaccgcgaac gacatcacgc catacaaccc gatcgacgcg agcaacaacg
     1921 cgagcccgcc aaataccgcc agcaggatcg cgcccatgcg cgccgcataa agcgagtttg
     1981 ccacgcgctc actcatcaat tccggattgc cgagtggtag gttcttttcg agtgactgga
     2041 cctcgttccg aatagcaccc gcaacgctcg acggatcgac gcgcgtgcgc acgtgcagca
     2101 ccatccctgt ctcatggttc tgctgcagag ccacatacgc taccggcgtc ggcgcttcac
     2161 cgagactgcg atacttgctc gtacgcacga cgccgacgat ctcgcgccac ggtccttttg
     2221 gcccattgaa actcagacgc ttgccaagcg cgtcctcatt cggaaaatgt cgccgcacga
     2281 aagcttcatt aacgatcacc acaggaggat ggtcgtcaga gtcctgcgcg ttgaagtcgc
     2341 gaccctgcaa cagcgcaata cccatcgttt gaaaatattt tgggccaatc acgttcgcgc
     2401 tgaccgactc attgttttcc gcaaccgggc ctgagttatc gctgcgaaat tgattctcgg
     2461 aaccggcgcg tccttcaatc atcaagctgc gcacggatga attgccgctt accgctgcga
     2521 tgcgggcgac gctcgctgat tcgactccgg gaatcgcttc gactcgctcg gtaacctggc
     2581 gataaaactc gcgtccctgc gctttcgtgt aacgaagcag gttgatattg agcggcaggc
     2641 caactatctt ctcggcgtcg aagcctggat cgattgtttg tgcttgtcgc aggctgcgta
     2701 gaaatagtcc cgccgcgatc agcaacacga ctgaaagcgc gacctgggtg acgacgagca
     2761 ggttgcgcaa agtaaatatg cgcgtgcgct cgaagaaggc cgtggaatcg tctttcaagg
     2821 cgggaatgag cgcgggcttt gacgagcgca gggcgggcgc gagtccgaag atgacgccgg
     2881 cagcgagcga gagtcctaac gtaaacagca ggacacgcag atcgatcgag aattgaggcg
     2941 tgatcggaag cgcgccggtt ggtggagtag ccgcgctgat cgttctggcg gtccaccatg
     3001 cgagtaacag tccggccgcg ccacctaagg tcgcgagcag cacgccttca gtcagtaatt
     3061 gtctaacgat ccgccatcgg gtcgcaccga tcgcgagtcg cacggcgatc tctttcgttc
     3121 gtgctgagct gcgtgcgagc agaagattgg cgacgttcgc gcacgcgata agcagcacga
     3181 taccgacgac gcccatcagt agtcgggcga cggacgaaag ctgttgccgt ccgtccggat
     3241 cgtcgctcgc actcaaaggc gagatcgtga tgccgcgacc gctgctctgt tgcgggtaag
     3301 ctacttcgag ttgtttggtt atcagagcaa gtgagctttg tgcctgcgcc tcgctgacgc
     3361 ggggtttgag tcgaccaaca ctgaacagcc agcgattagt gcgtacttgc agcaagtcgg
     3421 gattcatctc gcccgaaaat ccggcgcgcg gtggacgcat cgtcgcctgc atcatcatcg
     3481 gcacgtaaag atcgcgggtg atgccgagct gcaaaccgtc gaagcctttg ggaagcacac
     3541 cgatcacagt gaaagtattt ccgttgagca ggagctgtcg tccgatgatg tttggatcgc
     3601 cggcaaagcg tccctgccac aaaccctggc tgatgacggc caccggatgc gcgccgggag
     3661 tcttgtcgtc ttcagtcgtg atcacgcggc ccatttgtgc acgcacgccg agcacgtcga
     3721 agaagtttcc agtgacgatg gcgccggtga cgagatctgt ttgatcgttg ctgttgagac
     3781 tggtagtgat gccgccccac gcgatcaaac catcgaggac gttgttctga tcgcggaccg
     3841 cagcgtaatc gggataggag aagacgttgc cggctggtcc attgaaaaca aacacgagcc
     3901 gctctggatc ggaaacgggc ggcgtgcgca tcagcgtggc gttaacgaca ctgaagatcg
     3961 ccgtgtttgc tccaatccca agcgcgagtg tcaacacagc aatcacagta aaccccggtc
     4021 gctttaataa cccgcgcgca gcaaatcgaa tgtctttgat cattgattcc atacttgcca
     4081 aaccatccac agatttcacc gattattttt ctgtgtaatc tgtgaaatct gtggatggcc
     4141 tttcattcat acctgagcgc tacgagtgga tcgactttcg tcgccctgcg cgccggcaga
     4201 taacacgcca cgagggctac caccgacaac acccccggca cgagtacgaa cgtcgccacg
     4261 tcgtaaccac tgacgccaat caacaacgac gacaacacct gcgcgagtgc gagcgacagc
     4321 agtaacccga tcacgacgcc gaacgccgcg agccgcattc cctggcgcgt gatcatcttc
     4381 atcacgtcgc tgcggttggc gccgagcgcc atgcggatcc caacctcgcg cgtacgctgc
     4441 gaaacgacgt aagccatcac gctgtaaagc cccaccgcgc tcaataaaat cgccaccacg
     4501 gcaaacgcaa ccgagaaact cgccgccatg tttggcgccc acaacgcata cgtcttgtgc
     4561 tcgtccatcg ttttcacgcc cgacagcggc acacgacgat cgatcgcctg cacttcactg
     4621 cgaatcgcac cgacaagcga tcgcggatcc acgccagcac gcaccaccat cgcagtggga
     4681 gccggacggc gctggcccat cgagtagtag aaaaacggtt ttggatcttc ggctaacgtc
     4741 cggtacttgc cagtcttgac cacaccgaca acttcccacg catctttact atccacaccg
     4801 ataaagatgc gtttaccaac cgcgctctca cccggccaga gcatctcggc catctcttga
     4861 ttaacgatca cgacgcgctg cgttttcgac tgatctcgat cgtcgaagtc gcgaccgtca
     4921 agaatcggaa tctgcatcgt cttgaagtaa ccaggactga tgacagtcgt catgatcacg
     4981 cgtccggcac tacctttcgc gagcgtctcg ccttctttca aaaccggacc attggaattc
     5041 gagctgtcgc caagcggaag cgtgcgcgcg tacgagacag catcgacacc gggaacgtgc
     5101 gacacgcgct cgagcacctg cttgtaaaac tgtcgcgact gctcgtcttc gtaaccaatc
     5161 agcgtcggac tcaacgacac gatcagcccc ttcgcattat cgaaaccggg atccatcgtc
     5221 tgcgccttgc ggaaactctt gataaacaaa ccgccgcaaa ccagcaccac gagtgacaac
     5281 gcgacctgcg ccaccacgag gacattacgt aaccctaagc ctcgtctctt tcctcgtcgc
     5341 gctttctccg tctcgccttt caacaccgga acgagttcgg gattcgacga gtgccatgcg
     5401 ggcgcgagtc caaagatcaa acccgtgccg agcgaaacga cgagggtgaa caagagcgcg
     5461 cgtgagtcga gtgcgaagaa gttactgacg acgttgtatt cgaggatcgg aatgaaacct
     5521 tccatcagat cggtgaccca gacggcgagc agcaatccga gcccgccgcc ggcgattgaa
     5581 agcagcatgc tctcagttaa gagttgtcgc acgagacgcg cgcgattagc gcctaatgcc
     5641 agacgaatcc cgatctcctt gcgacgcccg gctgctcgcg cgagcatcag gttcgccacg
     5701 tttgcacacg cgatcaaaag aatgagcccg acgatcgcca ttgcgatcgc gcttgcgctc
     5761 ttgaacaccg gacccatctc ggcccaacga ccatcgatct cgccaagtac ctgaccttgc
     5821 gtagtggcag cgcgttcgtt tggataagac tgattcaaac gcgcggcgat cgtggcaact
     5881 tcagccgaag cctgttcgac tgagacaccg ggcttgagcc ggccaatcgc gttcatccag
     5941 tggctgccgc ggtcggcgag tatgccgggg ttgcggcgca gctcttcagc catcgagatc
     6001 ggggtccaga agtcgagcgc aagggcaaac ttcgtcccga cgaaataatc tggcgcgacg
     6061 ccgatcactt cgtaggcacg attattgagc tggaccgtct tgccgacgat gtttggatca
     6121 ccggccatgc ggcgttgcca gaagctgtgg ctcaacacga ccacgtgact cgcgccggct
     6181 gtcttatctt cgtctggtag aaacgctcga ccgaggatcg gtttgacttg cagcacatcg
     6241 aagtagtttg cggaaacaac ctgcccccac acgacgtcgt tctggttctc agtgtcgacg
     6301 gcggcctgga tcatgtcttc ggcagcaatc ccgctgaacg atgaactctg ttcgcggtat
     6361 tcgaggaaat cgggatagga aaactcatcc gtcgtgccgc ggtcatccga gatctcgagc
     6421 ggccggacca gctcggcagc attcggaacg ctaagcggtc gcatgagaaa cgcattcacg
     6481 ccactgaaga tcgcagtgtt ggccccaatc cccagcgcca gcgtgatcac cgccacgatc
     6541 gtcacgcccg gattcttcat caacatccga acgccatagc gaatgtcttt aattaaattt
     6601 tccatattct tatccgcgtt aatccggctt taagatccgt ccgatccgtg ttacccgatc
     6661 tgcttgtttt taagcagcgc cgatagcact cgaccgcgct cgtaatccga cttgatcgtc
     6721 tcgactgcct tcaggaacaa acctttcaac cgcgtatcac ccgtatacgc attcgacact
     6781 tccatgagca gcgttgcctt ctcataatcc gacgacatcg ccgcagccga atcgagcagc
     6841 aatcgcaaca cttcctcact ctggttcctc gccttcacga gggcgctcaa cacaccgcgg
     6901 cgctcgtagt cagacgagat cgtcttcgtc gacgacgcca cgagcacgag cacagcactg
     6961 ctgttggtct cctgcttcat gatcgccttg agcacacgac cacgctcgta gtccgacttg
     7021 atcgtggcga cggcatcgaa gaacggctgg agcgccgctt cccttccggt gagcatcggt
     7081 gcgacggccg tgagcaactg cgcctgttcg taatccgaag agatgttgcg cgccatctgc
     7141 cgcagcacat cacgcaacgc gccgccgtcg agattcgcct gtttcaaaag ctgctcgtag
     7201 taaattcgcc gcgcccaatc gccctggatc tgatcgatct ccgccagcac gcctgtcaca
     7261 ccacgctggc ccaggatcgt cttcactcgc ttctcagcat cgaaagcgcc ctgccggacc
     7321 gcgtcgagca caaactttgc cagccacgct ttcgcactcg catcgattgg ttttgcagca
     7381 ccgttgacat agaacagccg ctgcaactgc ccgctcgcat cacgcctgac ttcgtatcga
     7441 tacgactggc tatcgcgatg ctcctcgatg atcacccagc caccatcgga gacgctcttg
     7501 atgtcagtgt aatcgtcagt gaactcgagt ttgccgcgcg tctcgaagcg tcgtttcagt
     7561 ccgacatccg tgtcggtgta ttcccagcgc gtgacgcttt gagtctggtc acgagacgtc
     7621 tgcgcataga gttgcggcag cggcgacgcg ataacgatgg cgatgaatga ggcaagcagc
     7681 cagaggattc gtgccttaag cgagtggttg agcaacttca tgatttattt cccctttggt
     7741 ggtgtgtttg aggcttgcac gttgatgtgc acgcgcaagc ggcggccgat taatccgcgt
     7801 ccgtagttaa tgccgaaagc acaacgatcg atctcggtcg tggcgctgta gtaaacaaac
     7861 tcttcgccgc ttggtgagcg actgcgatcc atctcgttca cggccagagc gatgggtatg
     7921 gtcttgtctt tgatcgttag ctcgccttcg agatcgagcg agtcgcgatc tttgccgcgc
     7981 cggacgttcg tgctcctgaa ttcgatctcg ggaaacttct cgacgtcgaa gaagtcgcgc
     8041 aggagtaaat gtccatcacg ccgtttgttg ccggtgtcga tactgtcggg cctgattgtt
     8101 gcggtcacgg acgagcgact cacgtcgttc tcatccagaa cgatcacgcc ttcaagatcc
     8161 ctgaaccggc ctctcacctt gaaccacagg ttgcggatca cgaactcaac cgtcgtgtat
     8221 gccggatcga aatgccaggc atactgactc tgacgttcat tcattgccat cttgttaatc
     8281 ctgctgatcc tgtaatcctg ttgaagactt actcgtagcg gagcgccttc aaaggattaa
     8341 cacgagtcgc tcttcgtgcc ggcagatagc acgcagccag agtcacgaag aacaacccaa
     8401 aagacacacc gacaaaagtc gccgtgtcag tcgcagtaac ttcaaagagc aacgtagaaa
     8461 tcaatcgagt caacgcaaat gaagctatca atcccactac aaccccgatt acaccgagca
     8521 cgattccttt tcgcaccacg agcttcagca cgtcgaaacg ctgcgcgccg agcgccattc
     8581 gcactccgat ctcgtgcgtg cgctgcaaca ccgagtaaga catcacaccg taaatcccga
     8641 ccgcggccat gatgagggcg acgaaggaga agaccgtcaa cagcgacgca ctgaatcgcg
     8701 cgcgcgccac tgacgtcgat agcagagtcg ccatcgtgtt gacttcgccg atcggttgtt
     8761 gcggatcgat tgtgtggatg acgttgcgcg ccgcgcctgc aactgcattc gcatcgccgc
     8821 gtgtccgcaa catcaacgtc atgccgggat acaccaattc gacgtgaggc cagtagacca
     8881 tcggctcgat tgcgacatcg agtccgaggt gccggtggtc cgcaacgacg ccgatgatct
     8941 ccgacggcac gttgtcgtct ttcatgtaga tcgtgagctt gtggcccaaa ggattttggc
     9001 cgccgaggtt tttctgcacg aacgtctcgt tgacgagcac gacgtgccgc atctcagcca
     9061 cttcctgatc cgtgtagaag cgtccgagtt tcaaagggat ctgcatcgtt tggaagtaac
     9121 cggcgtcggt aacattcact cccgtgtgca attcccgatc ggg
//
LOCUS       DASSFQ010000279         9130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1763876, whole genome shotgun sequence.
ACCESSION   DASSFQ010000279 DASSFQ010000000
VERSION     DASSFQ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763876"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFH96_10120"
     CDS             complement(<1..761)
                     /locus_tag="VFH96_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6974370.1"
                     /translation="MNSFSQTKIPCTALVSLFLVLSLFTNSFSQTQQKEKPKLKDFGS
                     SLKKLKWDPQKNETVISQQGTRDDEDVITIETSLVTSDVLVTDRQGRPVRDLKATDFA
                     VSEDGVPQQIGHFVLGDNVNLSRSIVLIIDYSRSQFPYIADSIEAAGALVDQLGPKDR
                     MAIVTDDVELLVDFTNDKRELKKGLESLLERNRGAKSFLGVGGKRPRFGRSAQFSALM
                     ATLNEAFDDEDQNPVIVFQTDGDELEFLRNSIIVH"
     gene            complement(826..1713)
                     /locus_tag="VFH96_10125"
     CDS             complement(826..1713)
                     /locus_tag="VFH96_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6974371.1"
                     /translation="MAQLEYFSRVVQEKSFSKAADKVFRTQPAVSIAIRRLEEEIGLP
                     LLDRTQKTPVLTEAGKVVYDYAQRILGLRDQVGQAIAEMQSLKRGLIRVGANESTSLY
                     LLPELILKFRERHPEVKVEIFRLVSSRLPREVSERNIDFALMASEPADRDLEAFPLLN
                     DELVLIMSPKHPLAGRPSVKIKDLGNEPFVAHNVKSGSRVKVIEAFARQHTPLNITLE
                     LATIETIKRFVQKRVGLAFVPRMCVREELERGVLVSVPVRGLTHTRTLWAAHRRGSNF
                     SPAAAAFLEVLRQHAESGA"
     gene            1844..3583
                     /gene="ilvB"
                     /locus_tag="VFH96_10130"
     CDS             1844..3583
                     /gene="ilvB"
                     /locus_tag="VFH96_10130"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET6974372.1"
                     /translation="MIAQPEIENRVATSTAPAEMPTGAEMLIQALVHEGVDTIFGYPG
                     GAVLHIYDELWRARDRITHYLVRHEQGAVHMAEGYARATGRVGVVLVTSGPGATNAVT
                     GIANAYMDSTPIVVITGQVPKHLIGTDAFQEVDTVGITRPCVKHNYLVREAQDLPAIV
                     REAFHLARSGRPGPVVIDIPKDVSAARSDYSRLDHVSFPFSTRLARVDENSINQAVQQ
                     ILNAKRPVLYVGGGIVNSGAVDSLLSFAELLRLPVTPTLMGLGGFPSSHPLCLGMLGM
                     HGTYAANMAVAESDLLVALGVRFDDRVTGKLATFAPHAHVIHVDIDPANIGKNVTPTL
                     ALTGDVGQVLNQFLALVQEQSKERLEAWWDQISGWQLSQPLRFSGSQHQIKPQTAIRE
                     LHRLTNGEALIATDVGQHQMWVAQFYPFARPRQLITSGGLGTMGFGLPAAIGAQLALP
                     DQLVVAVVGDGGFQMTNQELATAVQYDLPVKVLIMNNGYLGMVRQWQEMFYDRTYSEV
                     DISVAPDFVKLAEAYGATGFRVQRPAELREVMEAWLRHEGVAIMDVVVAKEENVFPMV
                     PAGGDARAMITHA"
     gene            3599..4123
                     /gene="ilvN"
                     /locus_tag="VFH96_10135"
     CDS             3599..4123
                     /gene="ilvN"
                     /locus_tag="VFH96_10135"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET6974373.1"
                     /translation="MQDSQDSKRHTLSIIVADEPGELSRIVGLFSARGFNIETISVGE
                     SLDPRWSRVTIVTRGDERTIQQIVKQCERLARVREVQDVTTRPHIEREMALITVSAKA
                     GDERQEVLSLVSVFRAKVVDISHTQLIVEASGNKEKVDTFIELLRPIGVTDITRTGHI
                     AINRLAARADANTA"
     gene            4135..5151
                     /gene="ilvC"
                     /locus_tag="VFH96_10140"
     CDS             4135..5151
                     /gene="ilvC"
                     /locus_tag="VFH96_10140"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET6974374.1"
                     /translation="MNIYYDKDANLDLIRQKQIAVIGYGSQGFAQANNLKDSGCKVTV
                     GLRTGSSSWDKAERAGLKVLPVAQAAAEADIVMMLVPDELAPEIYVREVAESITPGKY
                     LSFSHGFSIHYRFIQPPEDANVFMVAPKGPGHLVRHEYQRGAGVPCLLAVHQDPTGDT
                     QQIGLSYASAIGGGRAGVIETTFREETETDLFGEQAVLCGGLTSLIKAGFETLTEAGY
                     APEMAYFECLHEMKLIVDLLYEGGISEMRYSISNTAQYGDMTRGPIVVNNETKNRMGS
                     LLKDIQSGAFARDWMNEAKNGRPRFQQLDDEAREHPIESIGARLRTMMPWLAQSRLVD
                     KASN"
     gene            5148..6821
                     /gene="ilvD"
                     /locus_tag="VFH96_10145"
     CDS             5148..6821
                     /gene="ilvD"
                     /locus_tag="VFH96_10145"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET6974375.1"
                     /translation="MNSKQRSHAITRGVERTPNRAMLRAVGFTDTDFQKPIIAVANGY
                     STITPCNAGLDRLTERAVATLREAGTMPQTFGTITVSDGISQGTEGMKYSLVSREVIA
                     DSIETVCQAQSVDGLLAIGGCDKNMPGAMIAIARLNIPALFVYGGTIKPGHLQGEYLT
                     IVSAFEAVGKFCAGKISEDQLVEVERHACPGIGSCGGMFTANTMSAAIEAMGLSLPFS
                     ATLSADSDEVVENAAAAAEVLVRAVQKQILPQQLLTREAFENAIAVVMATGGSTNAVL
                     HLPAIAHAAGIRLTLDDFESIRERVPVLCDMKPSGRYLATDLHKAGGIPQVMKVLLAH
                     GLLHGDALTITGETIEQVLAAVPENPRNDQDVIRQWDNAVYPQGHLVILRGNLALEGA
                     VAKVSGLKNRVITGRARVFDSEESCLHAILSAEIKPGDVVVIRYEGPKGGPGMREMLA
                     PTSALIGAGLGDSVGLITDGRFSGATYGMVVGHVAPEAAVGGNLALVNEGDSITIDAD
                     RRLLQVNISDDELARRRENWRPPAPRYTTGVLAKYAALVSSASLGAVTD"
     gene            6874..7419
                     /locus_tag="VFH96_10150"
     CDS             6874..7419
                     /locus_tag="VFH96_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974376.1"
                     /translation="MAIRIENQYEGSLPKGALEQIESAFDSLPREHTRGIERIRLVPF
                     ITDPRIKGAFQATELPGLYHPRQGPKGAWLELAINVLLQPDKPLHKRVIPRLSFKSNL
                     VALVFSLVGQHYHITLKHSLKKTQLEPAVRAYTEKQLKAWNEKKHSFRAKLFKPLQPT
                     FERWAKSLQKRAAAEKKRQAS"
     gene            complement(7476..8315)
                     /locus_tag="VFH96_10155"
     CDS             complement(7476..8315)
                     /locus_tag="VFH96_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET6974377.1"
                     /translation="MSKRGLPTGLQMRHDAHYVEELSQHRPQQVGRMIAVDKLDPNPE
                     QPRTEFGDLTELTASIAEKGVLEPLLVKPNRLTGRWMIIAGERRWRSAQKAGLKEVPC
                     VEMEVDEGTVAEIALIENMQRKDLTVWEEADGLLALCERFGYTHDDVARKVGKSRTTV
                     SEALAIARIPDDVREVCRSADINAKSSLLQIVRQPDDDSMRRLAKDIATRGLNRNDAR
                     EARRQEMGPRVVPDNKPYTFKYASPEKDFNIEVKFRASQVSDAEVALILRAVADEIDE
                     VQE"
     gene            complement(8308..9081)
                     /locus_tag="VFH96_10160"
     CDS             complement(8308..9081)
                     /locus_tag="VFH96_10160"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6974378.1"
                     /translation="MATLQRMVIAIANQKGGVGKTTTAINLAAAFARKGKRVLLLDLD
                     PQANSSISFLDPRAVERSIYELMMDGQAPVEDSVYQSPVENLHIVPARINLAKLEAKL
                     VGDFDAPFRLKDRLEPFRQQYEVIVIDTPPTLGLITVNALVAASHVLIPIQSSYFALE
                     GTDDLLETIEKVKARPNPNLELLGVLVTLHDKRTTLAREVHEQIKTVFGPKLFDTVIT
                     KSVRLEESPAYKESIFSFAPNSSGAIEYGKLCEEVISRV"
BASE COUNT         2023 a         2527 c         2605 g         1975 t
ORIGIN      
        1 tcatgaacga tgatggaatt gcgcaggaac tcaagctcgt cgccgtcagt ttgaaacacg
       61 atgaccggat tttgatcttc gtcgtcaaag gcctcattca acgtcgccat cagcgcactg
      121 aattgcgcac tgcggccaaa acgcgggcgc ttaccgccaa ctccgagaaa acttttagct
      181 ccgcggtttc tctcaagcag tgattccaga cccttcttaa gctctcgctt atcgttagtg
      241 aagtccacta gtagctcgac gtcgtccgta acgatcgcca tccggtcctt tggaccaagc
      301 tggtccacta aagcacccgc tgcttcgatg ctgtcggcga tatatggaaa ctggctgcgg
      361 ctgtaatcga tgatcagcac aatcgaacgc gacagattaa cgttatctcc caacacaaag
      421 tgaccaatct gctgcggaac accatcctcg ctgactgcaa aatcagtcgc tttgagatcg
      481 cgcactggcc ggccttgtct atcagtaacc aatacatcag acgttaccag cgacgtttcg
      541 attgtaataa cgtcttcatc atcgcgagtt ccctgctgac tgatgacagt ttcgtttttc
      601 tgcggatccc atttcagctt cttgaggctc gagccgaagt ctttgagttt gggcttttct
      661 ttctgctgag tttgtgaaaa tgaattcgtg aataaactca gaacgaggaa aagcgaaacg
      721 agcgcggtgc aggggatctt ggtttgtgaa aagctgttca cgatactgcc gttaattcag
      781 ggactaagca gagacatctt agctcaaatc ggcacggcag ataagtcatg cgccactttc
      841 agcgtgctgg cgcagaacct cgaggaaagc agcggcggca ggtgagaagt tggagccgcg
      901 gcgatgggcg gcccagagcg tgcgcgtgtg cgtcaggcct cgcaccggaa cggaaaccag
      961 aactcctcgt tcgagctcct cacgcacaca catgcgcggc acaaacgcca gaccaacgcg
     1021 cttttggacg aagcgcttga tcgtctcgat cgtcgcgagc tcgagcgtga tgttaagcgg
     1081 cgtatgctga cgtgcaaaag cttcgatcac ttttacacga gagcctgact ttacattgtg
     1141 agcgacaaac ggctcgttac caaggtcctt gatctttacc gaaggtcgtc cggcgagcgg
     1201 atgtttggga ctcatgatca ggaccaattc atcgttcagc agtggaaacg cttcgagatc
     1261 gcgatcggcc ggctctgacg ccatcaacgc aaaatcgatg tttcgctccg acacctcacg
     1321 cggcagacgg ctcgagacga gacgaaagat ctcaaccttg acttccggat gccgctcgcg
     1381 aaatttcaga atgagctccg gcaacaggta gagcgacgtg ctctcattcg cgcccacccg
     1441 gatcagtccc cttttcaggc tttgcatctc ggcaatcgcc tgccccacct gatctcgcag
     1501 cccgagaatg cgttgcgcgt agtcgtagac caccttgccg gcctcggtaa gcacgggcgt
     1561 tttttgtgtt ctgtcgagca gcggcaaacc gatttcttcc tcgagacggc gaatcgcgat
     1621 actcaccgcc ggttgcgtac gaaagacctt atcggcagct ttggaaaagc tcttttcctg
     1681 cactactcgc gagaaatatt ccagttgggc catttccata ggcgtaaact tttaccacac
     1741 ggcacggtca acggtcgatg aatcctgctt attattatct taaaagaata aattttacaa
     1801 agagtgaatc acaaggttag agtcttcgcc cacagagcga gccatgattg cccaaccgga
     1861 aatcgagaat cgagttgcca cgtcgacagc accggcggag atgcccaccg gcgcggagat
     1921 gctgatccag gcattggtcc atgaaggcgt cgatacgatc tttggctacc cgggtggcgc
     1981 cgtgctccac atctacgacg agctctggcg ggcgcgcgat cgaatcacgc actaccttgt
     2041 gcgacacgag cagggcgcag tgcacatggc tgaaggttac gcgcgcgcga cgggtcgagt
     2101 tggtgtagtg ctcgtcacgt ctggtcccgg cgcgacaaac gccgtgacag gcatcgccaa
     2161 cgcctacatg gattcaacgc ccatcgttgt gataaccggg caggttccaa aacatctcat
     2221 cggcactgac gcttttcagg aagttgacac tgtcgggatc acgcgaccgt gcgtgaaaca
     2281 caactatctc gtacgtgaag cgcaggatct gccggcgatc gttcgtgaag cgtttcacct
     2341 cgcgcgctcg ggccggcctg gaccagtcgt gatcgatatt ccgaaggatg tcagcgccgc
     2401 gagatcagat tattcccgat tggaccacgt gagttttccc ttctcgaccc gacttgcacg
     2461 tgtggatgaa aactctataa accaagcggt tcagcagatt ctcaatgcaa agcgcccagt
     2521 gttatacgtc ggtggaggaa tcgtgaattc gggcgccgtc gattccctgc tctcgtttgc
     2581 cgaactattg cggctgcccg taacgccgac attgatggga ctgggtggct ttccgagctc
     2641 gcatccgctt tgtctgggaa tgctcgggat gcatggcacg tatgccgcca acatggctgt
     2701 ggcggaaagc gatttacttg ttgcccttgg cgtcaggttt gatgaccgcg ttacgggtaa
     2761 actggcgacg tttgcgcctc acgctcacgt gatccacgtc gatatcgatc cggcgaacat
     2821 cggcaagaat gtcacaccaa cgctcgcgct cactggtgac gtggggcagg tgttgaatca
     2881 gtttctcgcc ctggtccagg agcaaagcaa ggagcgactc gaggcgtggt gggatcagat
     2941 aagcgggtgg caactgtcgc agccgctgcg cttctcgggc tcacaacacc agatcaaacc
     3001 gcaaactgcc attcgcgagt tgcatcgact gacgaacggc gaagcactca tcgccaccga
     3061 cgtgggccaa catcagatgt gggtagctca attctatccg ttcgcgcgac cacgacaact
     3121 gattacgagc ggcggactgg gaacgatggg attcggtttg ccggcggcga ttggcgcgca
     3181 gcttgcgcta cccgatcaac tggtcgtggc tgtcgtcggc gatggtggat ttcaaatgac
     3241 gaaccaggag ctggcgactg cggtgcaata cgatctgccg gtgaaggtcc tgattatgaa
     3301 taacggatac ctcggcatgg tacggcagtg gcaggagatg ttctacgacc gcacttattc
     3361 ggaagtcgat atttcagttg cgcctgattt cgtgaagctg gcggaggcgt atggcgcgac
     3421 gggttttcgg gtccagcgac cggcggagct gcgcgaggtg atggaagcgt ggctacgaca
     3481 tgaaggggta gccatcatgg atgttgtcgt ggctaaagaa gagaacgtgt ttccgatggt
     3541 gcctgcgggg ggcgatgcgc gcgcgatgat tacacacgcg tagacaggat tacacgattt
     3601 gcaggattca caagattcca aacgacatac gctgtcgatc attgttgctg atgagccggg
     3661 agagttgtcg cggatcgtcg ggctgttcag cgcgagaggt ttcaatatcg agacgatctc
     3721 ggtcggcgaa agtctcgatc cgcgctggtc gcgcgtcacg atcgttacgc gcggcgatga
     3781 acgcacgatc caacagatcg tcaaacaatg cgaacgactg gcgcgcgtcc gtgaggttca
     3841 ggacgtcacc actcgcccgc acatcgaacg agaaatggcg ctgattaccg tctcagcaaa
     3901 agccggagat gagcgtcagg aagtgttgag tctcgtgagc gtgtttcgcg cgaaagttgt
     3961 cgatatctcg catacgcaac tcatcgtcga agcgtccggc aataaagaaa aggtcgacac
     4021 atttatcgag ctgctgcggc cgatcggcgt caccgacatc acacgcacgg gacacattgc
     4081 tatcaacaga ctggctgcac gcgcagacgc aaacacagca taagaggcac acaaatgaac
     4141 atttactacg ataaagacgc gaacctcgat cttattcgtc agaaacagat cgccgtgatc
     4201 ggctatggca gccagggatt cgcgcaggcc aacaacctaa aagactcggg ctgcaaagtc
     4261 accgtcggcc ttcgtaccgg cagttcctcg tgggacaaag ccgagcgcgc cggtctgaaa
     4321 gtgttgccgg tggcccaggc cgctgccgag gcagacatcg tgatgatgct cgtgcccgac
     4381 gagctcgcgc ctgagatcta cgtgcgcgag gtcgcggagt cgatcacgcc gggcaagtat
     4441 ctctcgttct cacacggttt ctcgattcac tacaggttta tccagccgcc ggaagatgcg
     4501 aatgtgttta tggtggcgcc gaaaggaccg gggcatctcg tgcgtcatga gtaccagcgc
     4561 ggcgcgggcg ttccatgcct gctagctgtt caccaggatc cgacaggtga tacgcaacag
     4621 atcggtttgt cgtatgcatc agcgatcggc ggcgggcgcg ccggtgttat cgagacgact
     4681 tttcgcgaag agactgaaac agatctcttc ggcgagcaag cagtactctg cggcggtctg
     4741 acgtcgctga tcaaagcagg cttcgaaacg ttaacggaag cgggctacgc gcccgagatg
     4801 gcctacttcg aatgcctgca cgagatgaaa ctcatcgtcg atctgcttta tgagggcggt
     4861 atctccgaaa tgcgttattc tatatcgaac acagcgcagt acggtgatat gactcgtggt
     4921 ccgatcgtag ttaacaacga aacgaagaac cgtatgggaa gtctgctgaa ggacattcag
     4981 tcaggagcgt ttgcgcgcga ctggatgaac gaagcaaaaa atggacgacc gcgttttcaa
     5041 cagctcgacg atgaagcgcg tgagcatccg atcgagagta ttggcgcgcg cctgcgtacg
     5101 atgatgccgt ggctcgcaca aagtcgtctc gtggataagg ccagcaattg aactcgaaac
     5161 aacgcagtca cgcaatcaca cgcggcgtgg agcgaacacc caatcgtgcc atgctccgcg
     5221 ccgtcggctt tacagatacc gattttcaaa aaccgatcat cgcagtcgct aatggctaca
     5281 gcacgatcac gccctgcaac gccggcctcg atcgtctcac cgagcgcgcg gtagcaacac
     5341 tccgtgaagc cggtacgatg ccgcaaacct tcggcacgat cacggttagc gacggcatct
     5401 cgcaaggcac ggaaggaatg aagtattcgc tcgtgtcgcg cgaggtcatt gccgattcga
     5461 tcgaaacggt ttgccaggcg caatcagtcg acggtctgct ggcgattggt ggctgcgaca
     5521 aaaacatgcc cggcgcgatg atcgcgatcg cgcgtctcaa cataccggcg ctgtttgtgt
     5581 atggcggcac gatcaagccc ggtcatttgc agggtgaata tttgacgatc gtgagcgcat
     5641 tcgaagcggt cgggaagttt tgcgccggaa agatcagcga ggatcagttg gttgaagtcg
     5701 agcgtcacgc ttgtccgggc atcggctcgt gcggcggcat gttcactgca aacacgatgt
     5761 cagccgcgat cgaagcgatg ggtttgtcgc ttcccttctc tgcgacgctg agtgcggata
     5821 gtgatgaagt cgttgagaac gcagcagccg cggcagaagt actcgtgcgt gcggtgcaaa
     5881 aacagattct gccgcaacaa ctactcacac gcgaggcatt cgaaaacgcg atcgcagtag
     5941 tgatggccac cggaggctcg acgaatgccg tgttgcacct gcccgcgatc gcccatgcgg
     6001 ctggcattag actcacgctc gacgatttcg agagtattag agaacgggtg ccggtgcttt
     6061 gcgatatgaa accttcgggc cggtaccttg cgacagatct gcacaaggcc ggcggcattc
     6121 cgcaagtgat gaaggtgcta ctcgcgcatg gcttgctgca cggcgatgca ctgactatca
     6181 ctggcgagac tatcgagcag gtcttagcgg ctgtacccga gaacccacgc aacgatcaag
     6241 acgtgatacg ccagtgggac aatgcggttt atccgcaagg gcatctggtg atcctgcgtg
     6301 gcaatctcgc gctcgaaggt gcggtcgcaa aagtctcggg cctgaagaac agggtcatca
     6361 ctggccgggc gcgagtcttt gattcagaag aatcgtgtct gcacgcaatc ctgtctgctg
     6421 agatcaaacc aggcgatgtg gtcgtgatcc gttacgaagg tccgaaaggc ggacccggca
     6481 tgcgcgagat gctggcgccg acttcagcct tgatcggtgc tgggctcggc gattcagttg
     6541 ggttaataac cgatggacgc ttttccggtg ccacgtatgg aatggttgtt ggccacgtcg
     6601 caccggaggc ggcggttggt ggcaatctag cgctcgtcaa cgaaggtgat tcgatcacga
     6661 tcgacgccga ccgcaggctg ttgcaggtaa atatctcgga tgatgagctc gcgcgacggc
     6721 gtgaaaactg gcgtccaccc gcgccgcgtt atacaaccgg cgtactcgca aaatatgcag
     6781 cgctcgtcag tagcgcgagc ctcggcgctg taacagatta aataccctca cttgaccgtc
     6841 ctcatgacct tcccctaaag tgactcattt cgcatggcaa tacgtatcga aaaccaatac
     6901 gagggttcgc tgcccaaagg cgcgctcgaa cagatcgaaa gcgcttttga cagcctgccg
     6961 cgtgaacaca cgcgcggcat cgagcgaatc cggcttgtgc cttttattac cgatccgcgc
     7021 atcaaaggcg cttttcaagc gaccgagctg ccggggcttt atcatccgcg ccaaggcccg
     7081 aaaggcgcgt ggctcgagct cgccatcaat gtcctgctgc agccggacaa gccgcttcac
     7141 aagcgcgtca ttccacggct gtcgttcaag agtaatctcg tcgcgctcgt gttttccctc
     7201 gtcggccagc actaccacat cacgctgaag cactcgctca agaagacgca gctcgagcct
     7261 gcggtgcgtg cgtacactga aaagcagttg aaggcgtgga acgagaagaa gcacagtttc
     7321 cgagcgaaac tcttcaaacc tctgcaaccg acgtttgaac gttgggcgaa gtcgttgcag
     7381 aagagggcgg cggctgagaa gaagagacag gccagttagt ttatccacag attacgcaga
     7441 ttgtttatgg catctgtgta atctgtggat gaatattatt cctgcacttc gtctatctcg
     7501 tcggctacgg ctcgcagtat caacgcgact tccgcatcac tcacctgcga agcgcgaaac
     7561 ttcacttcaa tattgaaatc cttctcaggc gaagcgtact tgaacgtgta gggcttgttg
     7621 tcgggaacga cgcgcggtcc catctcctgg cgtcgcgctt ccctcgcgtc gttacgattc
     7681 aaaccacgcg tcgcaatgtc tttcgcgagc cgccgcatcg agtcgtcatc aggctggcgc
     7741 acgatctgaa gtagcgaaga cttcgcgttg atatcagccg agcgacacac ttcccgcacg
     7801 tcgtcaggaa tacgcgcgat cgccaacgcc tcactcaccg tcgtccgact cttcccgact
     7861 ttgcgcgcca cgtcgtcgtg cgtataacca aaccgctcgc agagcgccag cagaccatcg
     7921 gcttcttccc aaaccgtcag atccttgcgc tgcatgtttt caatcagcgc gatctctgcg
     7981 acggtgcctt cgtcgacttc catctcgacg cacggcactt ccttcaatcc cgctttttgc
     8041 gcagagcgcc agcgacgctc gccggcaatg atcatccagc gtccggtgag tcgattcggt
     8101 ttgacgagca gcggctcgag cacgcccttc tcggcaatcg acgcagtgag ctcggtgaga
     8161 tcgccgaatt cggtgcgcgg ctgttccggg ttcggatcca gcttgtccac ggctatcatc
     8221 ctgccgacct gctgcggccg gtgttgtgac agttcttcga cgtaatgcgc atcgtggcgc
     8281 atctggaggc cggttggcaa tcccctctta gacacggcta attacctctt cgcaaagttt
     8341 tccgtattcg attgctccgg atgagttcgg agcaaagcta aagatcgatt ccttgtaggc
     8401 gggcgactcc tcgagtctga cgctcttcgt gatcacagta tcgaaaagtt ttggaccaaa
     8461 cactgttttg atctgttcgt gaacttcgcg cgcgagcgtc gtgcgtttgt catgcaaggt
     8521 cacgagcacc ccgagcagct cgagattcgg attcggacgc gctttgactt tctcgatcgt
     8581 ctccagcaga tcgtctgtgc cttcgagtgc gaagtacgac gattggatcg gaatcaacac
     8641 gtgtgaagcc gcgacgagtg cgttgacggt gattagtccg agcgtcgggg gcgtatcgat
     8701 cacgatgact tcgtattgtt gccggaacgg ttcgagacga tccttgagcc ggaaaggagc
     8761 gtcgaaatca ccgacgagct tggcttcgag tttggcgaga ttgattctgg ccggaacgat
     8821 gtgcaggttc tcgaccggac tctggtacac cgaatcttcc actggcgcct ggccgtccat
     8881 catcaactcg tagatcgagc gctcgactgc gcgtggatcc agaaacgaga ttgagctatt
     8941 ggcttgtgga tcgagatcga gaagtaggac gcgttttccc ttacgggcaa aagcggcggc
     9001 tagattgatg gcggtagtgg tcttccctac cccacctttc tggttggcga tggctattac
     9061 cattcgctgc aaagtggcca actatcgctc catagctaac taatccttga cgcagagact
     9121 acattggtaa
//
LOCUS       DASSFQ010000280         9426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2222859, whole genome shotgun sequence.
ACCESSION   DASSFQ010000280 DASSFQ010000000
VERSION     DASSFQ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222859"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFH96_10165"
     CDS             <1..498
                     /locus_tag="VFH96_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET6974379.1"
                     /translation="ALEPDAHGLTVLPFWSGERSTGWHASARGGIFGLQQQTKPFEIY
                     RAVLESIAYRFALIARALDEVAPNATIAASGNALRSSPVWLQIIADVLGRPLQLGGPA
                     EASSRGAALLALEAVGKIATIEEDQFAIDRVFEPDMSRHARYQEGLARQEDLYNRLYD
                     HKRHK"
     gene            590..2617
                     /gene="tkt"
                     /locus_tag="VFH96_10170"
     CDS             590..2617
                     /gene="tkt"
                     /locus_tag="VFH96_10170"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6974380.1"
                     /translation="MSAPVQKENSNITPELDQLCINTIRALTLDAVQKAESGHPGLPL
                     GAAPMAYVLWTRFLRHNPRNPKWENRDRFLLSAGHGSMLIYSLLHLTGYDLPLEELKN
                     FRQWGSKTPGHPEYGLTPGVEITTGPLGQGFANGVGMAMGAAHLAAKFNKENFPLVDH
                     YVYAIVSDGDLMEGVASEAASLAGHLKLGKLIYLYDDNHVTIEGFTSLAFSEDVPKRF
                     EAYGWHTQTVADGNNLDEIEQAIRAAQAVTDKPSLISVKTVIGFGMPTQGTRKAHSDA
                     PGEEAVRETKRHLGWPEDKQFYIPDEAFAHYREAIPRGEQLESEWHELVKNYEEANPE
                     EAKTWRAMMSGELPDGWEDHLPKFEDAKAVATRVASGEVINALAPVLPMLIGGSADLG
                     VSNNTDIKSSHSFEAGSYDGRILHFGVREHAMGSTLTGISLNGGLIPYGGTFMTFSDY
                     MRPAIRLAALSEVQAIYVFTHDSIGLGEDGPTHQPIEHLAALRAIPHLFVIRPADSLE
                     VSEAWRIAILRRHAPTALALTRQKVPVIDRNKFAKADGLRRGAYVLTDAPAPRLILMA
                     TGSEVSLALEAWQKLQDEGVAARVVSMPCWELFEEQPQEYRDQVLPPSVSARLAVEAG
                     VRQGWDRYVGPQGDVICLDRFGASAPGDVAMKNLGFNVDNVMEHARALITS"
     gene            2698..3165
                     /gene="rpiB"
                     /locus_tag="VFH96_10175"
     CDS             2698..3165
                     /gene="rpiB"
                     /locus_tag="VFH96_10175"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET6974381.1"
                     /translation="MRIAIGADHGGYPLNERIIEELRHAHHDLIDFGTHDGSIPDDYP
                     DYAKQVGEAVQNGAAEIGILVCGSGVGAAVAANKLYGIRAALCSDTYSAHQSREHDDC
                     NVLCLGARVVGVELALDILRAFVAARFTGEERHRRRLAKIHALETNFKAPGNT"
     gene            complement(3123..4097)
                     /locus_tag="VFH96_10180"
     CDS             complement(3123..4097)
                     /locus_tag="VFH96_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HET6974382.1"
                     /translation="MTRIKTRIIINHSAARARRLWPTIQKQLDAARVEYQAYETKRPG
                     DATRQTRAALRSGVESVVVVGGDGTLSETAEGFFEFNDDLDVHPAPINPNVTLALLPA
                     GTGNDFARGLLGKRAPLQDWIETVIARSRGEATVRTMDVLYGRCNDYEKPFICFNAST
                     MGIGGETAGRVAAQGKFMRNFSGEFRFVFAAVGALAAWRERRVRVTVDGRTVADGPMN
                     LVAVANALYCGGGMMLSPEARIDDGKLDVITASGLSRANVVTELSRVHTGGHVNNPKV
                     TITQGTIARIDTFLEQDAMPLEADGNVRGVTPVQFQVLPGALKFVSSA"
     gene            complement(4110..5816)
                     /locus_tag="VFH96_10185"
     CDS             complement(4110..5816)
                     /locus_tag="VFH96_10185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6974383.1"
                     /translation="MRKRSFAPGIFFLVIALAIPALIIAQQNRSARRATGPDRPAATG
                     GAATKTGPNGPAIKSDLAEALAVIQSNHIDGRKLDYNAIFKSSISGMLSVLDPHSTYF
                     DPIEYASFRTEQRSEYFGIGATIEDLRDGKEVNTFIRATFPESPAARAGLRFGDRITA
                     VDGNSMQGKTYPEVRKFLLGPRGTTVKVTVEHPATKQSETVNIVRDAVSLPSIAQAYM
                     LKPGVGYVAMTGGFNLTTADEFDQALKDLHGKGMNMLVLDLRGNRGGLLIQAVRVANT
                     FLQRGQMIVSQRGRFRDSSQSYAAVNENPDGVPVVVLVNGDTASAAEIVAGALQDHDR
                     ALIIGETTFGKGLVQFPFQLDYDSALLLTIAKYFTPSGRLIQRDYSNGGFYNYYFPGG
                     LAADKKEQTPATPAGPESHTDTGRAVYGGGGISPDEVVKPGTISAAERNLRDVLFAFS
                     LELTTGRIQGFEQYKVQRAIEFDHDLVNEDYPVTDALFAALKKFAAAKPNYKTTPEQL
                     DKLRGFAEIQLRYNLLSAAYGYRTATQVFNDGDPQIGRAVDAMPRAKELAQAASRARA
                     RG"
     gene            6043..7422
                     /locus_tag="VFH96_10190"
     CDS             6043..7422
                     /locus_tag="VFH96_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6974384.1"
                     /translation="MSTTLNRDSLLSFANSHRDEYESLLRRFVETPTVSVDPNHLEDI
                     RKGVELTVETIEGYGGKVEVYRAEKGNPVIHGVFGNEKNLPTVTVYNHIDVQPASKET
                     EPWDTEPFEFTRKGDTYYGRGTTDDKGPALSALFGARAAIEAGIGVNIRFLWEFEEEI
                     GSPNFEKIITKAKSDLRTDSVVVSDTVWVSRNRPASSAGLRGLLGFILTLETGTVDTH
                     SGETGGASRNPIAELAQLITELYDVRTGKVKIKGFYDDVIPPSKKELQDWANCGFTTK
                     AFKKAHHLKKMRSEDPLEVMKRIWGMPTLEVHGIVGGYQGPGLKSIVPPRAELKASCR
                     LVPGQDPVKIRKQIAAAVKEKNPDVNVQFEHAAPAFRTVLEGELPQALKRAIKFSFGK
                     EAVFVRDGGTIGAMTSIEKVLKCPVLFLGLSLPEHGYHAPNENFDWQQASGGMVAFAK
                     YFEEIANLK"
     gene            complement(7489..8004)
                     /locus_tag="VFH96_10195"
     CDS             complement(7489..8004)
                     /locus_tag="VFH96_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6974385.1"
                     /translation="MRLRDYSTAGIIVPVSLTCRKMHAADVADAFHMLGDFLREDEHY
                     LASSQAYGDLGLQGLNNALDIFLEHPELGFVWMAYDENGVAGICVVCYAISTSMGSVV
                     AKLDDVSVKPDRRGKGTGSEMLNQLKEQLRREAVTRIDVAVHLQNPEARRFYEKAGFV
                     ALNEERLSCLI"
     gene            8046..>9426
                     /locus_tag="VFH96_10200"
     CDS             8046..>9426
                     /locus_tag="VFH96_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974386.1"
                     /translation="MRKITTTLFATALLLFTTTSAFGREARLVRYPHYHNGRIVFSYL
                     GDIWTADENGQNIQRLTVNRARDVYGRFSPDGKWIAFSSERNGNLDVFLIPSGGGNAK
                     QLTTHSADDIVLGWSSDSRSVLFSSNRGEDFMPQLYLVSTEGGMPWKAGTDMGIQASY
                     SPDGQKLAYNQKGQVYWRKYYRGSYSTDIMIMDVAAKKFTQVTDFDGEDSWPMWGHDG
                     FIYFVSDREGAGLTNIYRVSESGGKADRVTTFKNGDVRWPAISADGKVIMFEHDFGIW
                     KYDVGSKRATPIKLDIDAETQENLSEVQSFNSQADDYDLAPNSRRVAISIHGEIFTAP
                     VEEGDLKQITDGAARDRNVAYSPDGKWLSYVSDQSGREELFVAPVDGSAPATQLTDID
                     ALKNAYNWSPDSKEIAFASSDDKLRKLAVATKQVTELDSSRYGGFSAPVWSPDGKWIA
                     YSKPDASRNS"
BASE COUNT         2097 a         2643 c         2710 g         1976 t
ORIGIN      
        1 gctcttgaac ctgacgcgca cggattgacg gtgttgccat tctggtccgg cgaacgaagc
       61 acgggctggc acgccagtgc gcgcggcgga atcttcggac tgcaacaaca gaccaaaccg
      121 ttcgagatct atcgtgccgt gctcgagtcg atcgcatatc gattcgcgtt gatcgcacgc
      181 gcgttggatg aggttgcgcc aaacgcgacg attgctgcat caggtaatgc gctacgatcg
      241 tcgccggtgt ggttgcagat catcgctgac gttttaggtc ggccgttgca gcttggcgga
      301 cccgcggaag cgtcaagtcg cggcgctgcc ttgcttgcgc tggaggcggt gggcaaaata
      361 gcaactattg aggaagatca atttgcaatc gaccgggtgt tcgagcctga catgtcgcgc
      421 cacgcgcgct accaggaagg tctcgcacgc caggaggatc tctacaaccg actttatgat
      481 cacaaaaggc acaaataaag ccacaaaaag cacaagaaat acttttctag cttttgagct
      541 tttttgtgtt tctatttgtg ccttttgtgg ctaaaaggaa actaactaaa tgagtgcgcc
      601 ggtgcagaaa gaaaactcca acatcactcc cgaactcgat caactgtgca tcaacaccat
      661 tcgtgccctg acactcgacg ccgtccaaaa agccgagtcc ggccatcccg gcctgccgct
      721 cggcgccgcg ccgatggctt acgtgctgtg gacgcgtttc ctgcgtcata acccgcgcaa
      781 tccgaagtgg gagaatcgcg atcgcttcct gctttcggcg ggtcacggct cgatgctgat
      841 ctattcgctg ctgcacctga cgggctacga tctacccctc gaagaactga aaaactttcg
      901 ccagtggggc tcaaaaactc cgggccatcc tgaatacgga ctcacgcccg gcgttgagat
      961 cacaactggc ccgctcggcc agggctttgc gaacggcgtc ggcatggcca tgggcgccgc
     1021 gcacctcgcc gcgaagttca ataaagaaaa cttcccgctc gtcgatcatt acgtctacgc
     1081 gatcgtttcc gacggcgacc tgatggaagg cgtcgcttcc gaagccgcgt cgctcgcagg
     1141 tcatctgaaa ctcggcaagt tgatctatct ttacgacgac aaccacgtga cgatcgaggg
     1201 cttcacgagt ctcgcgttca gcgaagacgt gccgaagcgt ttcgaagcat acggctggca
     1261 cacgcagacc gtcgctgacg gcaacaatct cgacgagatc gagcaggcga ttcgtgcagc
     1321 ccaagcggtg actgacaagc cgagtttgat ttcagtcaag acagtgatcg gtttcggcat
     1381 gccgacgcag ggaacacgta aagcgcacag cgacgcgccg ggcgaggaag cggtgcgcga
     1441 aaccaagcgt catcttggct ggcccgaaga caaacagttt tacattcctg acgaagcgtt
     1501 cgcgcactat cgcgaggcga tcccgcgcgg cgagcagcta gaatccgagt ggcacgagct
     1561 ggttaaaaat tacgaagaag caaatccgga ggaagcaaaa acgtggcgcg cgatgatgag
     1621 cggggaactg cccgacggtt gggaagatca tctgccgaag tttgaagatg cgaaggcggt
     1681 ggcaacgcgc gtggcgagtg gagaagtgat caatgcgctc gcgccggtgt tgccgatgct
     1741 gatcggcggc tctgccgatc ttggcgtttc gaataacacc gatatcaagt cgagtcacag
     1801 tttcgaagcc ggatcgtacg acggccggat tctgcacttc ggcgtgcgcg agcatgcgat
     1861 gggatcgacg ctcacgggca tttcgctgaa cggcggtttg attccgtatg gcggaacgtt
     1921 catgaccttc tccgattaca tgcggcccgc gattcgtctt gctgcgcttt cagaagtgca
     1981 ggcgatttac gttttcacac acgactcgat cgggcttggt gaggatggac caacgcacca
     2041 accgatcgag cacctcgcgg ctttgcgcgc gattccacat ttgtttgtga ttcgtccggc
     2101 ggattcgttg gaggttagtg aagcgtggcg tatcgcgata ctcaggcgtc acgcgccgac
     2161 agcgttggcg ttgacgcgac agaaagtgcc ggtgatcgat cggaacaagt ttgcgaaagc
     2221 cgatgggctg agacgtggtg catacgtgct gactgatgcg cctgcgcctc gattgatatt
     2281 gatggcgacg ggatcggagg tttccctcgc gcttgaagcg tggcagaagt tgcaggacga
     2341 aggcgtggcg gcgcgcgtgg tgtcgatgcc gtgttgggag ttgtttgagg agcagccgca
     2401 ggagtatcgc gatcaggttt tgccgccgtc ggtgagtgca cggctcgcgg tcgaggctgg
     2461 tgtgcgacag ggttgggatc gatatgtcgg accgcagggt gatgtgattt gtttggatcg
     2521 gtttggggcg tcggcgccgg gtgatgttgc gatgaagaac ctcgggttta atgttgataa
     2581 tgtgatggag cacgcacggg cactgatcac aagttagaca ggatttacaa gatgtttcaa
     2641 gatgaatagg atttatcttg tgaatcctgt gcaatcttgt aaatcctgtc cacgactatg
     2701 cgcatagcaa tcggagcaga tcatggtggt tatccgctca atgaacgaat cattgaagag
     2761 ctgcgccacg cgcatcacga tctgatcgac ttcggcacgc acgacggctc gatccctgac
     2821 gattatcctg actacgcgaa acaagtcggc gaggctgtgc agaacggcgc cgccgagatc
     2881 ggaatactgg tctgcggtag tggtgtcggc gctgctgtcg ctgccaacaa actttacggc
     2941 attcgtgcgg ccctctgcag cgacacgtat tccgcgcatc aatcacgcga acatgacgat
     3001 tgcaacgtgc tgtgtctggg cgcgcgtgtg gttggagttg agctcgcgct cgatatcctg
     3061 cgagcgttcg tagcagcgag gttcaccggc gaggaacgac accgccgccg tctcgctaaa
     3121 attcacgcgc tcgaaacaaa tttcaaagcg cctggcaaca cctgaaactg caccggtgtc
     3181 acgccgcgca catttccatc tgcttccaac ggcatcgcat cctgctccaa aaacgtatcg
     3241 atacgcgcga tcgtgccttg cgtgatcgtg accttcggat tgtttacgtg accgccggta
     3301 tgtacccgtg atagctcagt cacgacattc gcgcgcgaca aacccgacgc cgtgatcacg
     3361 tcaagcttgc catcgtcgat acgcgcctcg ggcgaaagca tcatcccacc gccgcaataa
     3421 agcgcgttgg caacggcgac gagattcatc gggccatcag caacggtgcg gccgtcaact
     3481 gtcactcgca cgcgacgctc gcgccacgct gccagcgcgc cgaccgcagc aaacacaaaa
     3541 cgaaattcac cggaaaagtt gcgcataaac tttccttgtg ccgccacgcg tccggctgtc
     3601 tcgccgccga tgcccatcgt tgacgcgttg aaacagatga acggcttttc gtaatcgtta
     3661 caacgcccgt aaagcacatc catcgtgcga accgttgctt cgccacgaga acgcgcgata
     3721 acagtttcga tccagtcttg cagcggcgca cgtttgccca gcagtcctcg ggcaaaatcg
     3781 tttccggtac cggcaggcag aagcgctaac gtcacattcg gattaattgg cgcgggatga
     3841 acgtcgagat cgtcgttaaa ttcgaaaaag ccttcagccg tttcgctgag cgtgccatca
     3901 ccaccaacaa caacgaccga ttcgacacca gatctcaacg cggcgcgcgt ctgccgcgtc
     3961 gcatcaccgg gtcgcttggt ttcgtaagct tgatactcaa cgcgggcggc gtcgagttgt
     4021 ttctgaatgg tgggccacaa gcgccgcgca cgcgcggcgc tgtggttgat gataatccgt
     4081 gttttaatcc gtgttatctg tggttaggtt tatccacgag cccgagcgcg actagccgct
     4141 tgagctaact ctttagcacg cggcatcgca tcaacggcgc gcccgatctg cggatcgcca
     4201 tcattgaaaa cctgcgtcgc cgtgcggtaa ccataggccg ctgacaaaag gttgtagcgc
     4261 aactgtattt ccgcaaaccc gcgtagcttg tcgagctgct ccggcgtggt cttgtaattc
     4321 ggtttcgccg ccgcgaactt cttcaatgca gcaaagagcg catcggtgac cggataatct
     4381 tcgttcacga ggtcgtgatc aaactcgatc gcgcgttgca ccttgtactg ctcgaaaccc
     4441 tgaatgcgtc cggtcgtcaa ctcgagcgag aacgcaaaca gcacgtcgcg gagatttcgt
     4501 tcagccgcac ttatcgtgcc tggcttaacg acctcgtcag gcgagattcc gccgccaccg
     4561 taaacagcac gtccagtatc ggtatggctc tccgggcccg ccggagtcgc cggagtctgc
     4621 tcttttttgt cggcagcgag accgccgggg aagtagtagt tgtagaaacc gccgtttgaa
     4681 tagtcacgct ggatcagtcg acctgacggc gtgaaatact tcgcgatcgt cagcaacagc
     4741 gccgagtcgt aatccaactg gaacggaaac tgtacgagac cttttccgaa cgtcgtctca
     4801 ccgatgatca gtgcgcgatc gtgatcctgg agcgcgcccg caacgatctc tgccgccgaa
     4861 gcagtgtcgc cattgactag cacgacaacc ggaacgccgt caggattctc attcactgcc
     4921 gcatacgact gcgagctgtc gcggaaacgt ccgcgttgcg aaacgatcat ctgaccgcgt
     4981 tgcaggaacg tgtttgccac gcgcacggcc tgaatcaata gtccgccgcg attcccacgc
     5041 agatccagca ccagcatgtt catgcctttg ccgtgcagat ctttcagcgc ctggtcgaat
     5101 tcgtcagccg tggtcaggtt gaaaccgccg gtcatcgcca cgtaaccgac gccgggcttc
     5161 agcatgtagg cctgtgcgat tgacggcagc gaaaccgcat cgcgaacgat gttcacagtt
     5221 tcagactgct tcgtcgccgg atgctcgaca gtgaccttca cggtcgtgcc gcgcggaccg
     5281 agtaaaaact ttcgcacttc gggatacgtc ttgccctgca tcgaattgcc gtccacggcg
     5341 gtaatgcgat caccaaatcg cagtccggcg cgagcggcgg gcgattcagg gaaggtggcg
     5401 cgaatgaacg tgttgacttc cttgccgtcg cgaagatctt cgatcgtggc tccgataccg
     5461 aaatattcag agcgctgctc ggtgcggaac gaagcgtatt caattggatc gaaataggtc
     5521 gagtgtggat ccaggacgct cagcatgccg ctgattgacg acttgaagat tgcgttgtaa
     5581 tccagttttc tgccatcgat gtggtttgac tggataaccg cgagcgcctc ggcgaggtca
     5641 ctcttgatcg cgggtccgtt cgggcctgtc ttagtggccg cgccgcccgt ggccgccgga
     5701 cgatcgggac ctgttgctcg gcgggccgat ctgttttgct gagcgataat taatgcggga
     5761 attgccaggg caataacgag gaagaatatt cccggggcaa aagacctttt tctcacgcaa
     5821 ccctcctaaa ccgaagaact cggggacgcc agaataacac gaagcagcga accttttgaa
     5881 tgtacgcaga gcgccggacg aagttttctg caaaacgccg taggcatgta gatgcctcga
     5941 tttgccgata ggtcgcctgt taaataggac ctgtaggaca tacaggtcct atgctttatt
     6001 atttgcgcaa gctctcgcac gacagttcga cggaggatct gaatgtccac gacattaaac
     6061 agggatagtc ttttgtcttt tgccaactca caccgcgacg aatacgaatc gctgctgcgt
     6121 cgctttgtcg aaactcctac ggtttccgtc gatccgaatc atttagaaga catcagaaaa
     6181 ggcgttgagc tcacggttga aacgatcgag ggctacggcg gcaaagtcga ggtttaccgc
     6241 gccgaaaagg gaaatcccgt aattcacggc gtcttcggca acgagaaaaa cctgccgacc
     6301 gtgaccgttt ataaccatat cgacgtgcaa ccggcgtcga aagaaaccga gccgtgggac
     6361 accgagccgt ttgagttcac caggaagggc gacacttact acggccgcgg cactactgac
     6421 gacaaaggcc ccgccttgag cgcgctcttc ggcgcacgtg cggcgatcga agccggaatc
     6481 ggggtgaaca ttcgtttcct ttgggagttt gaagaggaga tcggctcgcc caacttcgaa
     6541 aagatcatca cgaaagcgaa gtcggatctg cgcacggatt ccgtcgttgt ttctgacacg
     6601 gtttgggtgt cgcgaaatcg tccggcgagc tccgctggat tgcgcgggct gctcggcttc
     6661 atccttacgc tcgaaactgg gactgtcgac acgcattccg gcgaaacagg cggcgcctcg
     6721 cgaaacccga tcgccgaact agcgcagctt atcaccgagc tgtacgacgt ccgcacgggc
     6781 aaagtcaaaa tcaaaggctt ttacgacgac gtgattccac ccagcaaaaa ggagttgcaa
     6841 gattgggcaa actgcggctt cacgaccaag gcattcaaga aagcgcatca cttgaaaaag
     6901 atgcgtagcg aagatcctct ggaagtgatg aagcgaatct ggggcatgcc gacgcttgaa
     6961 gttcacggca ttgtcggcgg gtatcagggg ccgggtttga agtcgatcgt gccgccgcgc
     7021 gcggagctga aggcttcgtg ccgtttagtg cctggccagg atccggtgaa gatcaggaaa
     7081 cagatcgcgg cggcagtgaa agaaaaaaat cctgacgtga acgttcaatt tgagcatgcg
     7141 gcgccggcgt ttcggacggt gctcgaaggc gagctgccgc aggcgctgaa acgtgcgatc
     7201 aagttttcgt ttggaaaaga agcggtgttc gttcgtgatg gcggcacgat cggcgcgatg
     7261 acgagtatcg agaaagtttt gaagtgcccg gtgctattcc tcggcttatc actacccgag
     7321 cacggctatc acgcgccgaa cgagaatttc gactggcagc aggcttccgg cggaatggtc
     7381 gccttcgcga agtacttcga agagatcgcg aacctcaagt aaaacttagc cacggataac
     7441 acggataaaa atccggattt aagatccgtg tcatctgtgg ttacggattt agatcaggca
     7501 tgagaggcgc tcttcgttaa gagctacgaa gccggccttc tcatagaatc ttcgcgcctc
     7561 gggattctgg aggtggacgg cgacgtcgat gcgcgtgacg gcctcgcgtc ttaattgctc
     7621 cttcaactga ttgagcatct ccgatccggt gccctttcca cgccggtcag gcttcactga
     7681 gacatcatcg agcttcgcca ccaccgaacc catcgacgtc gagatcgcat aacacaccac
     7741 acagatccca gcgacgccat tctcatcgta agccatccaa acaaaaccca gctccggatg
     7801 ctccagaaaa atatcgagcg cgttgttcag accctgcaga ccgaggtcac catacgcctg
     7861 actactcgcc aggtagtgct catcctcacg gagaaagtcg ccgagcatgt ggaaagcgtc
     7921 ggccacgtcc gccgcatgca tttttcgaca ggtcaaactc acgggcacaa tgatgccagc
     7981 ggtgctataa tcccgcaacc gcattccgag tagtttccat tcctttaacg cgcaggtcag
     8041 gagccatgag aaaaataacc accactctct ttgccacagc actgcttctt ttcactacca
     8101 cgtcagcatt cggccgcgaa gcgcgactcg tgcgctatcc gcattatcac aacggccgca
     8161 tcgtcttcag ctatctcgga gacatctgga ccgctgacga gaatggccag aacattcaac
     8221 gcctgacggt caaccgggcg cgcgatgttt acgggcgttt ctcgccggat ggaaagtgga
     8281 tcgccttctc gagtgagcgc aatgggaacc tcgacgtttt cctgattccc tcaggcggcg
     8341 gcaacgccaa acagcttacg actcactccg ctgacgatat cgtgctcggc tggtcgtctg
     8401 actcgcgctc ggtcctgttt agcagtaatc gtggcgaaga cttcatgccg cagctttatc
     8461 tcgtcagcac cgaaggcggc atgccgtgga aagccgggac tgacatgggc atccaggctt
     8521 cgtattcgcc ggacggtcaa aagctcgctt acaaccagaa gggccaggtt tactggcgca
     8581 agtattaccg tggctcatat tcgacggaca tcatgatcat ggacgtggcg gcgaagaagt
     8641 ttacgcaggt aactgacttc gacggcgagg attcatggcc catgtggggt cacgacggtt
     8701 tcatctactt cgtcagcgat cgtgaaggcg ctggtctaac aaacatctat cgcgtttccg
     8761 aaagcggcgg caaagccgat cgcgtgacca cgttcaagaa cggcgatgtg cgttggcccg
     8821 cgatcagcgc cgacggcaag gtgatcatgt ttgagcacga cttcggcatt tggaaatacg
     8881 acgtgggctc aaaacgcgcg actccgatca agctcgatat cgatgctgag acacaggaaa
     8941 acctgtcgga agtgcagtcg ttcaattcgc aggcggacga ctacgatctc gcaccgaatt
     9001 cacgacgcgt cgcgatctcg attcacggtg agatcttcac cgcgccggtc gaggaaggcg
     9061 atctcaagca gatcactgac ggcgctgcgc gcgatcgcaa cgtcgcctat tcgcctgacg
     9121 gcaagtggct ctcgtatgtt tccgatcaga gtggacgcga agagttgttt gtcgcgccgg
     9181 ttgacggctc agcgcccgcg acacagttaa cggacatcga cgcgctgaaa aacgcttaca
     9241 actggtcacc ggattcgaaa gagatcgcgt ttgcgtcttc ggacgacaag ctgcgcaagc
     9301 tcgcagtcgc cacgaaacag gtgacggagc tcgacagctc gcgctacggc ggtttcagcg
     9361 cgcctgtttg gtcgcctgac ggaaagtgga tcgcttattc gaagcctgac gcgtcgcgca
     9421 attctg
//
LOCUS       DASSFQ010000281         8331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_283411, whole genome shotgun sequence.
ACCESSION   DASSFQ010000281 DASSFQ010000000
VERSION     DASSFQ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283411"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3137)
                     /locus_tag="VFH96_10205"
     CDS             complement(<1..3137)
                     /locus_tag="VFH96_10205"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HET6974387.1"
                     /translation="MSPETTLLQDLLRRARSRRQLLLTLRGVAISVGVVAALLLLTGW
                     VAHRYRYNGSALLTLRIGALLTILATIYFALIRPLFKRISDAKLARLIEEHSPGTEDR
                     LVTAVEYSNGNAQSHISPALVNRLYQDANSVSGMLDVGNVIRRSRLMIYGGAALASLL
                     LFVGVLKFGPKEISQGVAQLVTPTTLAATTNAMSIKVRPGTARVPKGSDQDVIATLVN
                     FDSQQVTIFSRPVGSKVDFQGQVMEPAKAKTDFRFSIFNIQDSMEYFVESNNVRSEVF
                     KLNVVDLPYVKQLDLSLNFPAFTNLPTKAVEDGGDIAALKGTVTTITAKLSGKVKAAR
                     IVFADGKKTDMQLQGSDFVGQLTVNGDTSYYIELVSVDGEAYRGSNEYDVSVLSDQPP
                     VISFDKPGRDRKATNLEEVFTQARAEDDYGVVSMDLHFSVNGGAEKTVNLQNLTRESA
                     KSLTGAYTFFLEEYNLKPGDFISYFAKARDAASEATSDIYFIEVKPFEMEYKQSQQQG
                     GGGQGQGDQDQNALSRRQKDLIAATHRLMREGEKYTDQERKDGYEAVATGQEKLRTDT
                     LEFLDRMGRRLGDVDEGQKQVAEMAEHLRQAAKEMEAAPPPLRKESGKDALPPEQRAL
                     QRLLAADAIFREVQVAFGNQQNGGGGGGQREQQELAGLFELELDKMKNQYETVQRAQQ
                     QQAEKQKSEAEQRLEELARRQQAALEEQRRRMQQGSANGSGNQRQQQELIDETRKAAR
                     ELERLSRERRDPQMAELSRQLNQTADELQKAQASSRGNSGESVSQNERALDRLQQAQQ
                     RLQQMSGSNSQRGGQSTSSGRQQQISDLRQRAAQAASRQREIAKDMENLSRRGGQGSQ
                     DENSKRTREQLGERKDTLADSVNSLQQDLEQSARAMAGGSQGQQRTARQLKEAAESLA
                     RDRVADRIREGKQALNNGQQGKQGQEGQQNQQGGGGRSGEERAIERSLNNLSERLQAA
                     EQSAKGANGSSAEENLDRTRQLADNLDSLRRRLDERANGRNGQQQGQQQGQQKGQQQS
                     QQGQEGQQGQKGSESG"
     gene            complement(3212..5260)
                     /locus_tag="VFH96_10210"
     CDS             complement(3212..5260)
                     /locus_tag="VFH96_10210"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HET6974388.1"
                     /translation="MSFLTPLFFLGVAALAAPVLVHLVRRTRARRVQFPALVFVRQVP
                     QRTIRRRTLHNLLLLLLRCLAILLIVIAFTRPFFSGSSAAKDNTAAGATVILVDNSLS
                     MRREGMFAEAQRRAETALDEARNDEQIALVSFDKRYTVLNRFVADKNRVRLGITGLSG
                     GWDGTDYEQALRGAESLLSEIQTSGPKRIVMISDFQMPGWTAASATFKLSNNTQLTTL
                     DVGGNNPPPNVAITNVEARGVVFGQKYLDNLVVHVSNFSDAPKDHIQIDFQINDQTVE
                     KRDISLNSRDSRVVEFTGFNLNDGANRCTVDLTVGDFAPDNRFYFTLRRETPKQALII
                     EGASRGRSDSLHLQSALTTNDDLPFTFALKSAGSVDPVSISDYALVVLNDAGPISSAL
                     ADTLAKFVNAGGQMIVSTGPRTQADAFNNSLQQIAPATLREAVQTKAGESVAITEVKF
                     DHPIFEVFQESGRLAAANVIGYFRSEPRANTTVLARFEDGSPALVEGRTGKGRVLLFT
                     SSLGPSWNDLPLTPLYLPFIHQMVRYAGTREENAWYGLGQTFTVGKRQEQTPPAIDTP
                     GGARLSESRSTPDGDLLVTAREPGFYRLRYSSQPDFAAVNIDGAEGDFTKLNFAEFAV
                     GVTGGAGSAEGAAANRNLSDEQVEGRQRVWWSLLLVALLLLLAESFLARRTKMVKMIG
                     "
     gene            complement(5324..6226)
                     /locus_tag="VFH96_10215"
     CDS             complement(5324..6226)
                     /locus_tag="VFH96_10215"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6974389.1"
                     /translation="MTPTTATRFLDPELLARVGSLELLARAVVEGFMSGLHRSPFTGF
                     STEFTEYRQYNAGDDLRYLDWRLLARTDRYFIKKYRADTNTQCHLLVDTSASMNYAHE
                     SSVTKLQYAQFLAASLAYLLNRQQDAVGLVAFADKVHTHVPARNRTGHMRTIFGNLSM
                     LEAGGETHLAESLHQLAEILTRRGIVVIISDFYDRAERLQEAFQHLRFKGHDLVAFHV
                     LDQNELDFNFNEPVLLLEDAETQEQMPVLPDVVMSGYRERMRQHVEEMRRCAAANHVD
                     YEMLTTKQPLDFALFSFLSRRAGK"
     gene            complement(6299..7318)
                     /locus_tag="VFH96_10220"
     CDS             complement(6299..7318)
                     /locus_tag="VFH96_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6974390.1"
                     /translation="MAVEQLTISDEQLLDRLGSAREDLLREIRKAIIGQDEVVEQVLL
                     ALFVGGHSIITGVPGLAKTLLVRTIANVLDLSFKRIQFTPDLMPADITGTEIIEEDRA
                     TGHRRMQFIPGPIFANIVLADEINRTPPKTQAALLEAMQEGRVTVQGVSYDLPRPFHV
                     LATQNPIEMEGTYPLPEAQLDRFMFNVKIGYLPEEEEVAVVKQTTSPQDYDFKRLMSA
                     DEIIAFQRLVRQVPASDTVARYAVRLVNASRPGTPPAPDFVNKWVTWGASLRASQFLI
                     FGGKARAILRGRYNVSCEDVRAVAPAVLRHRVLLNFQAEAEKIDSDHVIKKLIESVPE
                     PKSGL"
     gene            complement(7388..>8331)
                     /locus_tag="VFH96_10225"
     CDS             complement(7388..>8331)
                     /locus_tag="VFH96_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974391.1"
                     /translation="NPLFVRAFMERRWFKMADLDAGFQRPKSPQDVPIAYFQASQVCE
                     FIAERYGFDAILRMLTMYRDKGRTPDVLQQDLKLSESDFDREFAAYIEAKVKPLQQAL
                     LTPTNNAASLSKAEVLKALETQDTFALRIRAAELLAADGDTQAAVTHYIRALELFPYT
                     SGKGNPYESLAKLLEQKGDREQAAKTLDALVKTDENNLEALKTIARLRFALGERQQAL
                     DALQASFFISPFDYKLHTQAGELSLDLKDYPKALTEFQVALALAPPNVAEANYNVAAA
                     FHALGRQPEAKRAVLRALEAAPRYEKAQELLLRIVGQ"
BASE COUNT         1635 a         2433 c         2338 g         1925 t
ORIGIN      
        1 ccgctttcag atcctttttg gccctgctgt ccttcctggc cttgttgact ttgttgttgg
       61 cccttctgtt gaccttgctg ctgtccctgt tgctgcccgt tgcgcccatt ggcccgttca
      121 tccaaacgac gacgcagcga gtcgagattg tcagcgagtt gccgcgtgcg atcgaggttc
      181 tcctccgcac tcgaaccatt cgcgcccttc gcactttgct cagccgcctg caaacgttcc
      241 gaaagattat tcaaactacg ctcgatcgcg cgctcttcac cgctgcggcc accgccgccc
      301 tgctggtttt gctgtccttc ttgtccctgc ttcccttgct gaccgttgtt caacgcctgc
      361 ttaccttcgc gaatgcgatc cgcaacacga tcgcgcgcga gtgactctgc cgcttccttc
      421 aactgccgcg ctgtacgttg ctggccctga ctgccgccgg ccatcgcacg cgctgactgc
      481 tcgagatcct gctgcaaact gttgacgcta tcggccagcg tgtctttgcg ctcgccaagt
      541 tgttcgcgcg ttcgtttcga gttttcatcc tgcgagccct ggccgccgcg ccgcgagagg
      601 ttctccatgt ctttcgcaat ctcgcgctgt cgtgacgccg cttgcgccgc ccgctgtcgc
      661 agatccgaaa tctgttgttg acgtcctgac gatgttgact ggccaccgcg ctgactgttc
      721 gagccgctca tctgctgtag acgttgctgc gcctgttgca gtcgatcaag cgcgcgttca
      781 ttctgcgaga ccgactcgcc ggaattgccg cgagacgaag cctgcgcttt ctgcaactcg
      841 tcggcagtct gattgagctg acgactcaac tctgccatct gcggatcgcg acgctcgcgc
      901 gaaagacgtt cgagctcacg cgctgctttg cgcgtttcat cgatcaattc ctgttgttgt
      961 cgttgattac cagagccgtt cgccgatccc tgctgcatgc gtcgacgttg ttcttcgagt
     1021 gctgcctgct ggcgtcgcgc gagttcttcg agacgttgtt cggcttcact cttctgcttt
     1081 tcggcttgct gctgttgcgc acgctgaacg gtctcgtact ggttcttcat cttgtccagt
     1141 tcgagctcaa acagaccggc gagctcctgt tgctcgcgct ggccaccacc accgccgccg
     1201 ttctgctgat tgccgaacgc gacctgcact tcgcggaaga tcgcgtcggc cgcgagcagt
     1261 cgttgtaagg cacgttgctc gggcggaagc gcgtctttgc ccgactcttt tctgagtgga
     1321 ggcggcgccg cttccatttc cttcgcagcc tggcgcaggt gttccgccat ctccgcaacc
     1381 tgcttttgac cttcatcaac atcaccgagc cggcggccca tgcgatcgag aaactcgagc
     1441 gtgtcagtac gcagtttctc ctggcccgta gcaacggctt cgtaaccatc tttgcgttcc
     1501 tgatcggtgt acttttcgcc ttcacgcatg agccggtgcg tggccgcgat cagatctttc
     1561 tgtcgacgcg agagggcgtt ctggtcctga tcgccctggc cttgtccacc gccgccctgc
     1621 tgctgcgact gcttgtactc catctcgaac ggtttcactt cgatgaagta gatgtcgctg
     1681 gtcgcttcac tcgccgcgtc gcgcgctttc gcgaaatagg agatgaagtc gccgggcttg
     1741 agattgtatt cctcgagaaa gaacgtgtac gcgccggtca acgacttcgc tgactcgcgc
     1801 gtgagatttt gcaggttgac ggtcttttcc gcgccgccgt tcacggagaa gtggaggtcc
     1861 atcgagacga caccgtagtc gtcttcggcg cgcgcctgcg tgaacacttc ttcgaggttc
     1921 gtcgcttttc gatcgcgccc gggtttatca aacgagatca cgggcggctg atcggagagc
     1981 actgacacgt catattcgtt cgagccgcga taagcttcac catcgacact gacgagctcg
     2041 atgtagtagc tcgtgtcgcc attcaccgtt aactggccga cgaagtctga tccctgcaac
     2101 tgcatgtcag tcttcttgcc gtcggcaaac acgatgcgtg cggccttcac tttccctgac
     2161 agcttcgccg tgatcgtcgt cacggtgcct ttgagtgcag cgatgtcgcc gccgtcttca
     2221 accgccttcg tcggaagatt tgtgaaggcc gggaagttga gcgacagatc gagttgtttg
     2281 acgtacggca gatcgacgac gtttagcttg aacacttcgg agcgaacgtt gtttgactcg
     2341 acgaagtact ccatcgaatc ctgaatgttg aaaatcgaga aacgaaagtc agtctttgct
     2401 ttcgcgggct ccatcacctg gccctggaag tcgactttcg agccgacggg acgagagaaa
     2461 atggtaactt gctgtgaatc gaagttgacg agtgtcgcga tgacatcctg gtccgagcct
     2521 ttcggaacgc gcgcggtgcc gggtcgcacc ttgatgctca tcgcgttggt tgtggccgcg
     2581 agtgtcgtcg gcgtgacgag ttgtgcgacg ccctgcgaaa tttcctttgg tccaaacttc
     2641 aatacgccca cgaagagcag caggcttgcg agcgcggcgc cgccgtaaat catcaggcgc
     2701 gaacggcgga tcacgtttcc gacgtcgagc attccggaaa cactgttggc gtcctggtaa
     2761 agacgattca cgagcgcggg cgaaatgtgc gattgcgcat tgccgtttga atattcaact
     2821 gcggtgacga gacgatcttc ggtgccgggc gagtgttcct cgatcagtcg agcaagcttc
     2881 gcgtcgctga tgcgtttgaa gagaggacga atgagcgcga aatagatcgt agcgaggatc
     2941 gtgagcaacg cgccgatacg taacgtcagt agcgcgctgc cgttgtatcg ataacgatgt
     3001 gcgacccagc cagtaagcag cagcagcgca gcaacaacac cgacgctgat cgcaacgccg
     3061 cgcaacgtga gtaacagttg ccgacgcgag cgtgctcgac gcaggaggtc ctgtaataga
     3121 gttgtctcag ggctcatgac ttctcccttt tatttagccg cggatgaacg cagattaacg
     3181 cagatctgcg tttattcgcg taaatccgcg gctaaccgat catcttcacc atctttgtcc
     3241 gtcgcgccag gaatgattct gccagcaaaa gcagcagcgc gaccagcaat aacgaccacc
     3301 aaaccctttg ccgtccctcg acttgctcat cactcaaatt ccgatttgcc gcagcgcctt
     3361 cagcactgcc tgctccaccg gttacgccta ctgcgaactc agcgaagttt aacttcgtga
     3421 agtcaccctc cgcaccgtca atatttacag cagcgaagtc aggttgtgag ctgtaacgca
     3481 agcgataaaa cccgggctcg cgcgcggtca cgagcaggtc gccgtcgggt gtgctgcgac
     3541 tctccgacag gcgcgcaccg cccggcgtgt cgatcgcggg tggcgtctgc tcttgtcgct
     3601 tgccgacggt gaacgtctgg cccaatccat accacgcatt ctcctcacgc gtccccgcgt
     3661 aacgaaccat ctgatggatg aacggcaggt acaacggtgt gagcggcaga tcgttccagc
     3721 ttggtcctaa tgacgacgta aacaacaaca cgcgtccttt tcctgtcctt ccttcgacga
     3781 gcgcgggact gccatcttca aaccgcgcca gcacggtcgt gttcgcgcgc ggctcggagc
     3841 ggaagtagcc aatcacgttt gcggctgcga gtctgccgct ctcctgaaac acttcgaaga
     3901 tcggatgatc gaatttcact tccgtgatcg caacgctctc gcccgccttc gtctgcactg
     3961 cttcacgcaa agttgccggc gcgatttgct gtagcgagtt gttgaaagcg tcagcctgcg
     4021 tgcgtggtcc ggtcgacacg atcatctgcc cgcccgcgtt tacaaacttc gcgagcgtat
     4081 cagcgagcgc ggaagagatc ggtccggcgt cattcagaac cacgagcgcg taatcggaaa
     4141 tgctcaccgg atcgaccgag ccggcgcttt tgagtgcgaa tgtaaagggc aggtcgtcgt
     4201 tcgtcgtgag cgcgctttgc aggtgaagac tatcgctacg accacgcgag gcgccctcga
     4261 tgatcaatgc ttgctttgga gtctcgcgtc gtaaagtgaa gtagaaacga ttgtcaggcg
     4321 cgaaatcacc cacagtgaga tcgacagtgc aacgattcgc gccgtcgttg agattgaacc
     4381 cggtaaactc caccacccgc gagtcacggc tgttgagact gatgtcgcgt ttctcgactg
     4441 tctgatcgtt gatctgaaaa tcgatttgga tgtgatcctt cggcgcgtcg ctgaagttac
     4501 tgacgtggac cacgaggttg tcgagatact tttgtccaaa cacgacaccg cgcgcctcga
     4561 cgttcgtgat cgcaacgttt ggaggtggat tatttccgcc gacatcgagc gttgtgagct
     4621 gcgtgttgtt tgagagtttg aaagtcgcgc tcgctgcggt ccagcccggc atctggaaat
     4681 cggagatcat gacgatccgt tttggcccag atgtttggat ctcgctcaat agcgattcgg
     4741 cgccgcgcag cgcctgttcg taatcagttc cgtcccagcc gccgctcagg ccggtgattc
     4801 cgagtcgcac tctgtttttg tctgcaacga agcggttcag aaccgtgtag cgcttatcga
     4861 aactcacgag cgcgatttgt tcatcgttgc gcgcctcgtc gagagcggtt tcggcgcgac
     4921 gttgtgcttc ggcaaacata ccttcgcgac gcatgctcaa cgagttgtct accaggatca
     4981 cagtcgcgcc ggctgcagtg ttgtctttcg cagcgctgct gccactgaag aatggacgtg
     5041 tgaacgcgat cacgatcagc aggatcgcaa gacagcgcaa cagcaacagc agcaggttgt
     5101 gcagcgtgcg acgccgaatg gtgcgctgcg gcacctgtcg cacaaaaacg agtgcaggga
     5161 actggacgcg acgcgcacgt gtgcgtcgca cgagatgcac gagaacgggc gccgcgagcg
     5221 ccgcgacgcc gaggaaaaat aatggcgtca ggaacgacat aaaacctgtc cacagattgc
     5281 acagattaca cagaaaacga tctgtgtaat ctgtggatga actttacttt ccagcccgac
     5341 gagacagaaa cgagaataat gcaaaatcga gcggctgttt ggtcgtcagc atttcataat
     5401 cgacgtggtt tgccgccgcg caacgtcgca tctcctcaac gtgctgccgc atccgctcgc
     5461 gatagccgct catcaccacg tcaggcagca cgggcatctg ctcctgcgtc tccgcgtctt
     5521 cgagcaacag cacgggctcg ttgaaattaa agtcgagctc gttttgatcg agcacgtgaa
     5581 acgcgacgag gtcgtggcct ttgaaacgca ggtgctgaaa tgcttcctga agacgctcgg
     5641 cccgatcgta aaagtcggag atgatgacga cgatgccgcg acgcgtcagt atttccgcga
     5701 gctggtgcaa cgattccgcg agatgcgttt cgccgcccgc ttcgagcatt gagagattgc
     5761 cgaagatcgt gcgcatgtgg cccgtgcgat tgcgtgcagg aacgtgtgtg tgcactttgt
     5821 cggcgaaagc gacgaggccg acggcgtcct gctgtcgatt cagtaaatat gcgagcgatg
     5881 cggcgagaaa ctgcgcgtac tgaagcttcg tcacggagct ttcgtgtgcg tagttcatcg
     5941 acgcgctcgt gtcgacgagc aggtgacact gcgtgttcgt gtcggcgcga tattttttga
     6001 tgaagtaacg atcggtacgc gcgagcagac gccagtcgag ataacgtaga tcgtctcccg
     6061 cgttgtactg gcgatactca gtgaactcag ttgagaaacc cgtgaacggc gagcgatgca
     6121 ggcccgacat gaaaccctcg acaaccgcgc gcgcaagcag ctccagcgag ccgacgcggg
     6181 cgagcaactc gggatcgaga aatctggttg cggtcgttgg ggtcatgtca gtcgtttaca
     6241 ggatttacaa aattttcaag atgcatcctg tttattctgt aaatcctgtc atcttgtctc
     6301 acaaaccact cttcggttca ggtacgctct caattaactt tttaatcacg tgatcggaat
     6361 cgatcttctc ggcctctgcc tggaaattta atagcactcg atgccgcagc actgcaggcg
     6421 caacggcgcg tacgtcttca cacgaaacgt tgtatctgcc gcgcagtatc gcgcgcgctt
     6481 ttccgccaaa gatcaaaaat tgggatgcac gcaacgacgc gccccacgtc acccacttgt
     6541 tcacaaagtc aggcgcgggc ggcgttcccg ggcggcttgc gttaaccagt cgcaccgcat
     6601 agcgcgcaac agtgtccgag gcaggcacct gtcgcacaag tctctgaaac gcgatgatct
     6661 catccgccga catcaaccgt ttgaaatcgt aatcctgcgg cgacgtcgtc tgcttcacca
     6721 ccgcgacttc ctcttcctca ggcaagtaac caatcttcac attgaacatg aatcgatcga
     6781 gttgggcttc gggtagcgga tacgtgcctt ccatctcgat cgggttctgt gtcgcgagca
     6841 catgaaacgg acgcggcaga tcgtaactga caccttgcac ggtcacgcga ccttcctgca
     6901 ttgcttcgag taaagccgcc tgcgtcttcg ggggcgtacg attgatctcg tcagcgagta
     6961 cgatgttggc gaagattgga ccaggaatga attgcatccg gcgatgaccg gtggcgcgat
     7021 cttcttcgat gatctccgtg ccggtgatat cggcaggcat taaatccggc gtgaattgaa
     7081 tgcgcttgaa agaaagatcg agaacgttgg caatggtccg caccagcaac gtttttgcaa
     7141 ggccgggaac gccggtgatg atcgaatggc caccgacgaa aagcgcgagc aacacctgtt
     7201 cgacgacttc gtcctgcccg atgatggcct ttctgatttc gcgcagcagg tcctcgcgcg
     7261 cagagccgag tctgtcgagc agttgctcgt cggaaatggt taactgttca acggccataa
     7321 gcttcctttt atccacagat ttcacagatt acacagaagg cattagtcag tctgcgcaat
     7381 ctgtggatta ctgtcctact attcttaaca acaactcctg cgctttctcg tagcgtggcg
     7441 cggcttcgag cgcgcgtagc acggcgcgct tggcttcggg ttggcggccg agcgcgtgaa
     7501 acgccgcggc cacgttgtag tttgcttcgg ccacgttcgg cggcgccagt gcgagcgcca
     7561 cctgaaactc cgtcagcgcc tttggataat ccttgagatc gagactcaac tcgccggcct
     7621 gcgtgtgcag tttgtaatca aacgggctga tgaaaaaact cgcctgcaaa gcgtcgagcg
     7681 cctgctgtcg ttcgccgagc gcgaagcgaa gacgcgcgat ggtcttcaac gcttcgagat
     7741 tgttttcgtc agtttttact aacgcatcga gagtttttgc agcctgttcg cgatcgcctt
     7801 tttgttcgag cagcttcgcc agcgactcgt atggattgcc tttgccgctt gtatacggga
     7861 agagctcgag cgcgcggatg taatgcgtga ccgcagcttg cgtgtcacca tcggcagcga
     7921 gcaactcagc ggcgcgaatg cggagtgcga aagtgtcctg cgtctcgagc gctttcagca
     7981 cttccgcttt cgatagtgaa gcagcgttgt tcgttggcgt aagcaacgcc tgttgcagcg
     8041 gtttgacttt cgcttcgatg tacgcggcaa actcgcgatc gaaatcactc tcactcaact
     8101 tcaagtcttg ttgcagcacg tcaggcgtac gccctttgtc gcgatacatc gtgagcatgc
     8161 gaagaatcgc gtcgaacccg tagcgctcgg cgatgaactc acaaacctgc gacgcctgga
     8221 agtaagcaat cggaacatcc tgcgggctct tcggtcgctg gaaaccggca tcgagatccg
     8281 ccatcttgaa ccagcgccgc tccatgaacg cgcgcacaaa cagcgggttc c
//
LOCUS       DASSFQ010000282         7349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1027441, whole genome shotgun sequence.
ACCESSION   DASSFQ010000282 DASSFQ010000000
VERSION     DASSFQ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027441"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFH96_10230"
     CDS             <1..577
                     /locus_tag="VFH96_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974392.1"
                     /translation="LMDRIVGGFQFTGIHRYRSGPALTPFLAGGQRDFLQLVGYLGNL
                     RPNVTGQPFYTDTAAGGVQYRYLNPAAFSRPLDFRNAPAFSLDGGVTVNPAYIAYYSD
                     PNRFFGNSAPTYGGLRAQPFFTEDFSLMKKTKITETTYFEIRAEFFNLFNRGRFGLPN
                     VNVDDINFGISARNADIFQPRRIQVGGRFVF"
     gene            677..2116
                     /locus_tag="VFH96_10235"
     CDS             677..2116
                     /locus_tag="VFH96_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974393.1"
                     /translation="MAQGQQTKQFVDEVDEHTYLGLNAVQAGDLKLAEQHFAAAVKAD
                     PNRASARNNYGAILMRTGRSALAAAEFEQSLKLDPNQPNALANLAQIRFDGGKPDDLR
                     TSFELFKRAFAIKPDVEIARALTVIGLRLQDNAAALTYYQSYAAAVDAQTPTARAELG
                     AALFEAGLLKEAEAELTAAVSLNPTDTDSVVRLARVYLASKNIAGAGRTLEASVARGN
                     DPAPVYALLAEIYEQSGHVENAIPAMRLAIQRDPQSEKYRFGYAILLTNSNAPGAAVI
                     RLEESLQTFPSSARLWFALGFANFKLDKNEEAERALRKAIELDSKFAPAFAYLGLLRA
                     KTGAYAEAITLYENALRADTKLAVVHHLIADAMLKQNADANIVEKHLRQSVAMDPTFT
                     PARLSLGKLFMRAQRWSDAVGELEQVVKLDPNVPEAYYQLGLAYGRLKRTADAQSAMA
                     TFKQLSDAEKKRENEELREVVKRLSNVRF"
     gene            2141..3811
                     /locus_tag="VFH96_10240"
     CDS             2141..3811
                     /locus_tag="VFH96_10240"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HET6974394.1"
                     /translation="MKRREFLAGTAAITAGILVERNGGLVHSSAAADSRIEVLLGEEI
                     GTINPNIYGHFAEHLGGVVYDGIWVGENSKVPNTGGIRNALVDHLKRIEAPVIRWPGG
                     CFADSYDWRDGVGPRARRPRRTNFWADAPEWPKNVTDGPWKFETNHFGTNEFLRFCQL
                     SGAEGYLAVNLRGLSAQEFYEWIEYCNSPAGSTTAAERRAASDMPARDPFRVKYWGVG
                     NESWGCGGNLTPEEYSVEYRRFTAAVPRYGVDLSFIASGSNGGDSSWTRGFFSRVAEK
                     NRNLFNGIYGFGLHHYAWNLGFGKTNDWFAAKGDAVKFTDAEHYELLHEADRMESLIN
                     EHWTIMGEYDRQHRVKLVVDEWGAWYKPGSEVNPTHLLGQQSTMRDALLAGLTLDTFN
                     RHADKVAMANVAQLINCLHSLFLAHEDKFIVTPTFHVFEMFMPHMGARAVRTIFSAPE
                     VQYTRADKPAEFWGLSGSASISAKQLTITVTNPHLSETREAEIAVRGARAGAVRAQLL
                     SMSDIHAHNSFENPNAVQPRNEQVAAAAGVITFRFPPASVTRLQIALD"
     gene            3938..4978
                     /gene="galT"
                     /locus_tag="VFH96_10245"
     CDS             3938..4978
                     /gene="galT"
                     /locus_tag="VFH96_10245"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET6974395.1"
                     /translation="MFWEERWHPLREEWVIIAAHRQNRPWSGETIEHNGGQLPEYVAD
                     CYLCPGNPRVSGARNDNYTTTFVFDNDHPCVGFEAPQTLQPPPGIYRNQPATGIARVV
                     CYSPNHSLALAELEPCAIKDLLAVWQDQYRELSARKEINHVLIFENKGEAVGVSNPHP
                     HCQIYATNFVFKTIETEARASKQHFAETGRILFQDVIRAEKEDGRRIICENESAIAFI
                     PYFARYAYEVFVSPKATHPSIATLSRDELQDFAALLKQVLVKFDNLWQMPFPYVMPLH
                     QAPTDGGDYSSFHFHIEFHPPLRRPNLLKYLAGPEIGGGNFLSDTAPEEKAAELRAQA
                     DTHYKHITREQS"
     gene            4975..6015
                     /locus_tag="VFH96_10250"
     CDS             4975..6015
                     /locus_tag="VFH96_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974396.1"
                     /translation="MSDVSSLLGPIRRIHERVRDAVVAECERAQLEQLTAVSDDDSEG
                     DTIYAIDRVSETLLVELFEQELASTAPLVLIAEGIEHGKLVLPRDATEADAVWRVIVD
                     PIDGTREVMYQKRSAWVLTGVAPNRGDDTNLQDIELAVQTEIPLLKQHLCDTLWAIRG
                     NGADGERFDRLTGERKPLAPRPSRAPTIRHGFAMVARFFPGAREELAAIDEEIVRGAL
                     GPVEPGKAHCFEDQYLSSGGQLYELMMGHDRFVADLRPLIESVLNRRGLVLGICCHPY
                     DVCTELIAREAGVIVTDEAGGPLRAPLRVEPDVTWVGYANEAIRREIEPHLREALEKR
                     GFSDSLRQDERD"
     gene            6087..>7349
                     /locus_tag="VFH96_10255"
     CDS             6087..>7349
                     /locus_tag="VFH96_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HET6974397.1"
                     /translation="MKTEYDLQDVTEFIDKLDKLESFFEPRTEITIARAPGRLDVMGG
                     IADYSGSLVLELPIAEATLVALQKDDARQLRVVSLLGDEALTFEMPLSDFEHNGEPIE
                     YDEAQRYFKSDRTRHWAAYVAGVFLVLSRERGVRFDRGARLLISSQVPEGKGVSSSAA
                     LEVATMSAVAAAFDIQVGPRDLALLCQQVENLVVGAPCGVMDQMTAACGEANHLLSLI
                     CQPAELQGLLKLPTDLAVWGLDSGARHSIGAGDYGSVRAGTFMGYRIIAELAGDDSRW
                     GGYLANLTPSEYEEKFAADLPESLSGDEFISRYGSTTDRVTKVESDRKYAIRAPTEHA
                     IYESFRVKKFAELLDKQKDQPSLGDKMAMGELMYASHQSYSACGLGSNGTDLLVELVK
                     TQGVDSGLFGAKITGGGSGGTVAVLGSSE"
BASE COUNT         1626 a         1987 c         2151 g         1585 t
ORIGIN      
        1 cttgatggat cgcattgtcg gtggtttcca gttcaccggc attcatcgct atcgcagcgg
       61 tccggcgttg acgccgtttc tcgcgggcgg gcagcgagac tttctccaac tcgttggtta
      121 tctcggcaac ttgcgaccca acgtcaccgg acagccgttc tacactgaca cggccgcagg
      181 tggcgtgcaa tatcgttatc tgaatccggc agcgttttcg cgaccgctgg atttccgcaa
      241 tgcgccggcc ttctcgctcg acggtggcgt aacggtgaat ccagcctaca tcgcgtatta
      301 ttccgatccg aaccgattct ttggtaactc tgcgccgact tacggtgggc tgcgcgcaca
      361 accgttcttc acggaagatt tcagtctgat gaagaagact aagatcaccg aaacgactta
      421 ctttgagatc cgcgctgaat tctttaatct cttcaatcgc gggcgctttg gacttccaaa
      481 cgtaaacgtt gacgacatca acttcggcat ctcagcacgc aatgccgaca tcttccagcc
      541 acgccgaatt caagtaggcg gaaggttcgt cttctagtga aagtagccac aaaaagcaca
      601 aaaggcacaa atgagtagaa cagtgctctt caacagaaac tcttttttgt gccttttgtg
      661 ccttatgtgc ttttttgtgg cccagggtca gcaaacgaag cagtttgtcg acgaggtcga
      721 tgagcacaca tatctcgggc tcaatgcagt tcaggccggc gatttgaagc tcgccgagca
      781 acacttcgcg gcggcggtca aagcggatcc aaatcgcgca tcggcgagga ataactacgg
      841 cgcgatcctg atgcgcaccg gaagatcggc tctcgccgcc gcggagtttg agcagtcgct
      901 caagctcgat ccaaatcaac cgaatgctct ggcgaatctc gcccagattc gttttgatgg
      961 cggcaagcct gacgatctgc gcacttcgtt tgagttgttc aagcgtgcgt ttgcaatcaa
     1021 gccggatgtt gagatcgctc gcgcgctgac tgtgatcggg cttcgtcttc aggacaatgc
     1081 cgcggcgttg acttactacc agagttacgc ggctgcggtt gacgcacaaa ctccgactgc
     1141 gcgtgcggag ctgggcgccg cgctctttga agcaggactg ttgaaagaag cagaagctga
     1201 gttgactgcg gcggtcagtc tcaatccgac agatactgac tcggtcgtgc gtttggcgcg
     1261 cgtctacctt gcgagcaaaa acatagccgg cgccggtcgc acgcttgaag catcggttgc
     1321 gcgcggcaac gatccagctc cggtttacgc gctattggct gaaatctacg agcaaagtgg
     1381 tcacgttgaa aacgcgatcc cggcgatgcg tctggcgatt cagcgcgatc cacaatctga
     1441 aaagtatcgt tttggatacg caatcttgtt aaccaattcc aacgcgcccg gcgcggcagt
     1501 cattcggctc gaggaatcgt tacagacatt tccctcatcc gcgcgtctct ggtttgcact
     1561 cggatttgcg aacttcaaac tcgataagaa cgaagaagct gagcgagctt tgcgaaaggc
     1621 gatcgagctc gattcgaagt ttgcgcctgc atttgcatat cttggtttgc ttcgggcaaa
     1681 aaccggcgcc tacgccgaag cgatcacgct gtacgaaaac gccctgcgtg ccgatacaaa
     1741 acttgccgtg gtccatcacc tgatcgcgga cgcgatgttg aagcagaatg ctgacgcgaa
     1801 catcgtcgag aaacatttgc ggcaatccgt cgcgatggat ccgacgttca cgccggcgcg
     1861 gctgtcactt ggcaagctat tcatgcgggc gcagcggtgg tccgacgctg tcggagaact
     1921 cgagcaggtg gtgaaactcg acccgaacgt gcccgaagct tattaccaac tcggcctcgc
     1981 ctacggtcgt ctcaaacgaa ccgccgacgc gcagtccgcg atggcaacct tcaagcagct
     2041 cagcgacgcc gagaagaaac gcgagaacga ggaactccga gaagtcgtga agagactaag
     2101 caacgttcgc ttttaaatcc gtctaatccg tggctcaaaa atgaaaagac gtgaattttt
     2161 agcagggaca gcggcgatca ccgccggcat tctggtcgag cgcaacggtg gtctggtgca
     2221 ttcgtcggcc gccgccgatt cacgtatcga ggtgctgctg ggcgaagaga tcggtacgat
     2281 caatcccaac atctacggtc acttcgccga acacctcggt ggcgtggtct acgacggcat
     2341 ctgggttggt gaaaactcga aagtgccgaa caccggcggt attcgtaacg ctcttgtcga
     2401 tcacctgaaa cgcatcgagg cgcctgtgat ccgctggccc ggcggctgct ttgccgacag
     2461 ttacgactgg cgcgacggtg ttggaccacg cgctcgccgt ccgcgacgca caaacttctg
     2521 ggccgacgcg cccgaatggc ccaagaacgt caccgatggt ccatggaagt ttgaaacaaa
     2581 tcacttcggc accaacgagt ttctgcgctt ctgccagttg tcgggagccg aaggctacct
     2641 cgcagtgaat ttacgcggtc tgagcgcgca ggaattttac gaatggatcg aatactgtaa
     2701 ttcgcccgcc ggttctacta cagctgccga acgaagagcc gcgagtgaca tgccggctcg
     2761 cgatccattt cgcgtcaagt actggggcgt cggcaacgaa tcgtggggtt gcggcggcaa
     2821 tctcacaccg gaagagtact cggttgaata ccggcgcttc actgctgccg ttccgcgtta
     2881 cggcgtcgat ctgagcttca ttgcttccgg ctcaaacggt ggcgactcaa gctggacacg
     2941 cggattcttc tcccgagtcg ccgaaaagaa tcgaaatcta ttcaacggca tctacggttt
     3001 tggtttgcac cattacgcgt ggaacctcgg ctttggcaaa acgaacgact ggttcgccgc
     3061 caaaggcgac gcggttaaat tcaccgacgc cgagcattac gaactgcttc acgaagcaga
     3121 ccgcatggag tcgctcatca acgagcattg gaccatcatg ggcgagtacg accggcaaca
     3181 tcgcgtcaag ctggtcgtcg acgaatgggg tgcgtggtat aaacctggat cggaagtcaa
     3241 tccgacgcac ttgttgggcc aacagtcgac gatgcgcgac gcgctgcttg cgggcctgac
     3301 gctcgacacg tttaatcgcc acgcagataa agtagcgatg gccaacgtcg ctcaattgat
     3361 caactgtctg cattcgctgt tcctggccca tgaagacaag ttcatcgtga cgccgacgtt
     3421 tcacgtgttc gagatgttca tgccgcacat gggtgcgcgc gccgtacgga caatcttcag
     3481 cgcgcccgaa gtacaataca cgcgcgctga taaaccggcc gagttttggg ggttgtcagg
     3541 ctctgcatcg atcagcgcca agcaattgac cataacggta acgaacccac acctcagcga
     3601 aacacgcgag gctgaaatcg cggtgcgtgg tgcgcgtgcg ggcgcggtgc gcgcgcagtt
     3661 gttatcgatg tccgacatac acgcgcacaa ctcgttcgag aatccgaacg ctgtgcaacc
     3721 gcgcaacgag caggtcgcag ccgctgccgg tgtgatcaca tttcgctttc cgcctgcgtc
     3781 ggtgacgcgg cttcaaatcg cgctggattg aggagggctt tctctgtgca acctctgtgt
     3841 tctctgtgtc tctgtggttt aaaggtttcg gaaagcagtt aaccacagag tcacagagaa
     3901 cacagaggtt acacagagaa gcatccacga caagactatg ttctgggaag aacgctggca
     3961 tccattacgt gaagagtggg taatcattgc tgctcaccgg cagaaccgtc cgtggagtgg
     4021 tgagacgatt gaacacaacg gagggcagtt acccgaatac gtcgcggatt gctacctctg
     4081 ccctggcaac ccacgcgtca gcggcgcgcg aaacgacaac tacaccacga ccttcgtctt
     4141 cgataacgat catccctgcg ttggtttcga agcgccgcaa acgctgcaac cgccgcccgg
     4201 catttatcgc aatcaaccgg cgaccggaat cgcgcgcgtc gtttgctaca gcccgaacca
     4261 cagtctcgca cttgccgagc tggagccatg cgcgatcaaa gacctgttgg cagtctggca
     4321 ggatcagtat cgcgagctga gcgctcgcaa agagatcaat cacgtgctga tctttgaaaa
     4381 caaaggcgaa gcggtgggtg tttcgaatcc gcacccgcat tgccagatct acgcgaccaa
     4441 ctttgtcttc aaaacaattg agacggaagc gcgtgccagc aagcaacatt tcgctgaaac
     4501 cggacgcata ctttttcagg acgtgattcg cgctgagaaa gaagatggtc gccgaattat
     4561 ctgtgagaac gaatcggcga tcgcattcat tccctacttt gcgcgctacg cttatgaggt
     4621 tttcgtctcg ccaaaggcaa cgcacccgag tatcgcgacg ctcagccgag atgagcttca
     4681 agacttcgct gcgttgttga agcaagtact ggtgaagttt gacaaccttt ggcaaatgcc
     4741 gtttccgtac gtgatgccgc tgcatcaagc gccgactgac ggcggcgact actcgagttt
     4801 tcattttcac atcgaattcc atcccccgct gcgtcgcccg aatctgctca agtacctcgc
     4861 cgggccggaa atcggcggcg gtaactttct ttctgacaca gcgccggaag aaaaagccgc
     4921 ggaactgcgc gcgcaagccg acacgcacta taagcacatc acgcgggagc agtcatgagc
     4981 gacgtgagta gcttgcttgg tccaattcga cggattcatg agcgagtccg tgatgcggtc
     5041 gtcgcggaat gtgagcgggc gcaactggaa caactcacgg cagtttccga cgatgattct
     5101 gagggcgaca cgatttacgc gatcgatcgg gtcagcgaaa cgctgctggt cgaattgttt
     5161 gagcaggaac tcgcgagcac tgcgccactc gttctcattg cggaaggtat cgagcacggc
     5221 aagctggttt tgccgcgtga cgcaactgaa gccgatgcgg tctggcgtgt gattgtcgat
     5281 ccgatcgacg gcacgcgcga ggtgatgtat cagaagcgca gtgcctgggt gttgaccggt
     5341 gtagcgccca atcgtggcga cgacacaaac ctgcaggaca tcgagcttgc ggtgcaaacg
     5401 gaaatcccgt tgctcaagca gcatctttgc gacacgctct gggctattcg tggtaacggt
     5461 gccgacggag aacggtttga tcggttgact ggagaacgaa agccactagc gccgcggcca
     5521 tcgcgcgcgc cgacgatccg gcatggtttc gcgatggtgg cgcgtttctt tccaggcgcg
     5581 cgtgaagagc tggcggcgat tgatgaagag atcgtgcgcg gtgcgcttgg tccagtcgaa
     5641 ccggggaaag cgcattgttt tgaggatcag tatctgtcat cgggtggtca actgtacgag
     5701 ttgatgatgg gccacgatcg ttttgtcgca gatctccggc cgctgattga atcggtgttg
     5761 aatcgccgcg ggctggtgct tgggatttgt tgtcatccgt acgatgtgtg tacggagttg
     5821 atcgcgcgcg aggccggcgt gattgtcacc gatgaggctg gtggtccgtt gcgcgcaccg
     5881 ctacgagtcg agccggacgt gacgtgggtt ggatacgcga atgaagcgat tcgacgtgaa
     5941 atcgaaccgc atttgcgtga agccttggag aaacgtgggt tcagcgacag tcttagacag
     6001 gatgagcgag attagaaaag aggattttca tgttaatcct ggaaaatctt ttgaatcctg
     6061 tctaaccatt attcgagaag tcgggcgtga agactgaata cgatcttcag gatgtgacgg
     6121 agtttatcga caagctcgat aagctggaat cgttctttga accacgaact gaaataacga
     6181 tcgcgcgcgc tccggggcga ctcgacgtga tgggtggcat tgccgattat tccggttcgc
     6241 tcgtgcttga gttgccgatt gccgaagcaa ccctggtcgc actgcaaaaa gacgacgcgc
     6301 gacaacttcg agtcgtcagc ttgctcggcg atgaagcgtt gactttcgag atgccgctgt
     6361 cagacttcga acacaacggc gagccgattg aatacgacga agctcagcga tacttcaaga
     6421 gcgatcggac acggcattgg gctgcttacg tcgccggtgt attccttgtg ctgagtcgcg
     6481 aacgtggcgt gcgtttcgat cgtggtgctc gtctactgat ctcgtcgcaa gtaccggaag
     6541 gcaaaggcgt cagctcgtca gcagcgctcg aggtcgcaac gatgagtgcg gtcgcggcgg
     6601 ctttcgatat tcaggttgga ccaagagatc tggccttact ttgccaacag gttgaaaacc
     6661 tcgtcgtcgg cgcgccgtgc ggtgtgatgg accagatgac agcggcatgt ggagaagcaa
     6721 accacttact gtcgctgatc tgtcagccag ccgagctaca gggcctgctc aagttgccca
     6781 cggatctggc agtgtggggt ctcgactccg gagcgcggca ttcaataggt gccggcgact
     6841 atggctcggt gcgagcagga acatttatgg gctaccgcat cattgcggaa ctcgccggtg
     6901 acgattcgcg ctggggtggt tatctcgcaa acctgacgcc gtctgagtat gaagagaagt
     6961 ttgcagccga tttgcctgag agtttatccg gcgatgaatt catttcgcgc tatggcagca
     7021 caaccgatcg tgtcacgaaa gttgaatctg atcgcaagta cgcgattcgc gcgccgacgg
     7081 aacacgcgat ttacgagagt tttcgagtga agaagtttgc cgagctattg gacaagcaga
     7141 aggatcagcc gagtctgggc gacaagatgg cgatgggcga gttgatgtac gcgtctcacc
     7201 agagttattc ggcgtgtggg ctgggttcga acggcaccga cttgctcgtc gaacttgtta
     7261 agacgcaggg ggtcgacagc ggactcttcg gcgcaaagat cactggaggc ggcagcggcg
     7321 gcacggtcgc agtgctcggc agtagtgag
//
LOCUS       DASSFQ010000283        22513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_284096, whole genome shotgun sequence.
ACCESSION   DASSFQ010000283 DASSFQ010000000
VERSION     DASSFQ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22513
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284096"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..849)
                     /locus_tag="VFH96_10260"
     CDS             complement(61..849)
                     /locus_tag="VFH96_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET6974398.1"
                     /translation="MYEHIQVAENEGILTIMLNRPDRLNAFIGHMRRDLAEALEHAGS
                     DRGVRVVIITGAGRGFCAGGDIAFMAELMKRRDAEEFSRILGAGRRVILAIRQMTKPV
                     IASINGPASGAGCNLALACDLRIASTNATFSQSFAKVGLHPDWGGTYFLPRLVTPNKA
                     CEMFFLGESIDAAEAMRLGIVNQVVAPEELESTTLQLAERLRASPPIALAAAKHAVYM
                     SQAAELEEMLRYETEAQLRCFESDDGHEGVHAFLEKREPRFTGH"
     gene            complement(851..1642)
                     /locus_tag="VFH96_10265"
     CDS             complement(851..1642)
                     /locus_tag="VFH96_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6974399.1"
                     /translation="MLKLDYEVKDGIALITLNDPPANTYSYEMMQELDRTILNARMDE
                     AVQVIVITGKGEKFFCAGADIQMLANVTPAYKYYFCLHANETLNRLEQTPKLVIAAIN
                     GHCVGGGLEVAMAADIRIARKGAGKMGLPEVSLGVLPGTGGTQRLVRIVGKSQAIEMM
                     ATGQLFDFERGLALGIVNQIFEAETVEHFAEMVQDYARQFTTPNKAAHAVGRIKRAVQ
                     TGSEIPFESALALERELQQQLFQSEDAKEGLDAYVNKRKPEFKGR"
     gene            complement(1648..2289)
                     /locus_tag="VFH96_10270"
     CDS             complement(1648..2289)
                     /locus_tag="VFH96_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6974400.1"
                     /translation="MAKSYDQKHEFILRTAARIFAEKSYHSTSMRDISRATNVSLAGL
                     YHYCKSKEELLFLIQDNCFGRVLERLEQRLEGVADPITKLGIFIENHLSFFAANMSEM
                     KVLSHEAESLGGDLYNHVSTRKDKYTQLARQILREVQETQQSQQPIDLTVATYALFGM
                     MNWIYNWYDPHGKLKVSDLASNVMRLFLNGFVPNGMCSSLALPSESPSVWSHG"
     gene            complement(2314..3711)
                     /gene="boxB"
                     /locus_tag="VFH96_10275"
     CDS             complement(2314..3711)
                     /gene="boxB"
                     /locus_tag="VFH96_10275"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HET6974401.1"
                     /translation="MNLYDRIPNNVNLSEDKKLQRALEKWLPNYLDWWREMGPEGFQE
                     KDVWLRTAVDVGSEGWAHFDYVKMPDYRWGIFLEPPQPDAKIGFGDNLGRPAYQEVPG
                     EFRNALRRIIVTQGDTEPASVEQQRQLGKTCPSLYDLRNLFQVNVEEGRHLWAMVYLL
                     HGYFGRDGRDEADELLERRSGNPDKPRILEAFNQPCDDWLNFFAFTMFTDRDGKYQLS
                     ALAESGFEPLARTCQFMLTEEAHHMFVGDTGLGRVCRRTAQLMKEGDVRELGGIPLEI
                     IQKYVNFWFSYSLDLFGGEISSNSADFFAAGLKGRYKERDQYSDHSATNENFTMTIVE
                     DGQLKTREVPLRNALNEVLRGEYVKDCEKGLARWNRVLAEEGASERLYLPHPRFHRHV
                     GEFAGHTFDLQGNLISREEFGSRKSEWLPTIEDREYVRSLMHPVFEIGKIANWIAPPA
                     AGIKGKPFDYEYVRL"
     gene            complement(3851..5479)
                     /gene="boxC"
                     /locus_tag="VFH96_10280"
     CDS             complement(3851..5479)
                     /gene="boxC"
                     /locus_tag="VFH96_10280"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HET6974402.1"
                     /translation="MSNPEILFQTAPEQYKHWRLKVEGPVATLSMDVQEDETLADGYK
                     LKLNSYDLGVDIELADAVQRIRFEHPEVRAVVVTSLKPRIFCAGANIYMLGSSSHAFK
                     VNFCKFTNETRCAIEDDSSHSGRRYIAALNGTASGGGYELAIACDEIYLVDDGNSAVS
                     LPEVPLLGVLPGTGGLTRLVDKRKVRRDRADVFSTLAEGLKGKRAKEWGLIDGYFPTS
                     KFQESIDARVKELVNAAAPAKGIKLPPLEFETTDDARNYKYVKLKLNRDQRYADLTVL
                     APEGPQPTTAEQIHELGAAYWPLQAYRELDNALLHLRVNEPLIGLVCLRTEGDAETVL
                     SIDQTLAANHDHWLVREIVLHMARVLRRLDLTAKSFFAIVEPGSCFAGNLLELLLASD
                     RSYMLMDSDASLAVSELNQGAFLMSNGLTRMQSRFLAEPEKLDEVFGDKKKFDTEEAE
                     DLGLITFAPDDLDWEDEIRVAIEERTSLSPDALTGMEASLRFAGPETMDTKIYGRLTA
                     WQNWIFQRPNAVGPEGALTNYGKPTQAHFDYKRT"
     gene            5647..5745
                     /locus_tag="VFH96_10285"
     tRNA            5647..5745
                     /locus_tag="VFH96_10285"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5681..5683,aa:Sec,seq:tca)
     gene            5792..6922
                     /gene="dprA"
                     /locus_tag="VFH96_10290"
     CDS             5792..6922
                     /gene="dprA"
                     /locus_tag="VFH96_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET6974403.1"
                     /translation="MIRDWVELNMTPGIGPRAAAKLLERFGSAEAVYKATRAELEQLR
                     LPPEAVDTIIARDLLPRAEAEITAVKQLGGDILLLDDGVYPSSLREIYDPPIVLYVKG
                     AWSDCLDRPCIGVVGSRRCSTYGQNAANMLARDLAERGVTVVSGFARGIDAAAHRGAL
                     EANGRTVAVLGTGIDEVYPREHKKLAAEILDRGGAFVSQFPLGTPPVSENFPYRNRII
                     SGLSLGVVVVEAAENSGSLITARLAMEQNREVFAVPGNITSRNSFGTNYLIKGAGAKL
                     VQQWQDIASELPQPVAASLLPPPFSERKNPVSDRLAFVPEGLSPAETCVFKLLTPDSP
                     AHVDWLFDKSKLPISELTAALLSLEIRELIRALPGRCFVRKL"
     gene            6923..9421
                     /gene="topA"
                     /locus_tag="VFH96_10295"
     CDS             6923..9421
                     /gene="topA"
                     /locus_tag="VFH96_10295"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET6974404.1"
                     /translation="MAKNLVIVESPAKAKTINKYLGKDYLVKASIGHIKDLPSKGLGV
                     DVEHNFEPTYELIPDSKKRNNKRIVAELKKAAKEAEAIYLAADPDREGEAICQHLKEE
                     IVPKRPAKPAFRVMFNEITKRAVNEAFEHPRQIDENLVDAQQARRVLDRLVGYKVSPL
                     LCRTIGGRLSAGRVQSVALRMVVEREREIEKFVKTEYWTIAANVSSKLPPAFDSRLFK
                     IDEQTVKTSGFDQDLKKNEIVINDQTKADAIVAEAQQQQFVVTDVTTKERKRNPVPPF
                     ITSKLQQEASRKLGFAVKKTMMLAQKLYEGVELGDEGSVGLITYMRTDSTRVSDAALG
                     EVRDYINGQYGGNYLPEKAVHYRSKKDAQDAHEAIRPTEVARTPDSLASYLKPEELKL
                     YRLIWQRFVASQMMPAVFDQTTIDIQAGRFIFRATGSVQKFDGFLKIYQEGRDEKAED
                     DDEAERSLPLVEKGENLKLNTITPEQHFTEPPPRYTEATLVKALEEKGIGRPSTYAAI
                     MSTIQEREYVEKLQGRFHPTALGKTVNDLLIDGGFDDLFNETYTARMEEELDDIEEGK
                     IRWTKALREFYDKFEGDLKQFQHYIKDIKEKNVPTEEVCLKCNTPGMVQKWGRFGPYL
                     KCLSCEATRDAEPVNGSNGASASSEPQANGEESAEPEVCELCGKPMQLKRGRFGPFLG
                     CTGYPECRNIRKIGKSGVAAPPPVPIDEKCPVDGAQLVKRVGRFGEFISCSNYPKCKY
                     IKQETVGVSCPRPGCKGEIVVKKSKRGKAFYGCSEYPNCNVVYWDKPVLETCPDCNAP
                     FLLEKTTKKQGTFRYCANEDCPSREKEQKTQKVS"
     gene            9561..11273
                     /locus_tag="VFH96_10300"
     CDS             9561..11273
                     /locus_tag="VFH96_10300"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6974405.1"
                     /translation="MIHRRIAAVVLLLIFIISPASLLSQQTPAPTPDPNDPIQRVKDE
                     GMNRSQVMQTLSYLSDVIGPRLTASPGMKRANEWTRDQLTKFGLQNAHLEAWGPFGRG
                     WTLKRFSAQVTDPTAIPLIAYPKAWSPGLSSPLTADVVYFDAKTEADFEKFKGKLNGK
                     IVLTAPVRDVSAHFEALGTRLTEKDLLTLADAPEPRAGGNRGNFAANPQFRAAFELAN
                     AKMRFFQSEGAALLIDPSRGDGGTMFVQSAAVPSPPRDPNATTANPFARGIPPYDKSA
                     PKTMPQLVVAIEHYNRIVRMLQAGEAVKMNVDLTVAWQDADLMGYNTIAEIPGTDLKD
                     EIVMIGGHMDSWHSGTGATDNAAGCAVAMEAVRIIQALGIKPRRTIRIALWSGEEQGL
                     LGSRAYVADHFGTLQSPATSAAPATGGLNNGMGGGNGNGAPAAPTLITKPEYEKLSAY
                     YNLDNGTGKIRGVYLQGNEAVRSLFRQWLAPFRDLGATTLSIANTGGTDHLSFDGIGL
                     PGFQFIQDEIEYDTRTHHSNQDVFDRIQGDDMKQAATIMAAFLYQTAMRDDKIPRKPA
                     PGAR"
     gene            complement(11328..12542)
                     /locus_tag="VFH96_10305"
     CDS             complement(11328..12542)
                     /locus_tag="VFH96_10305"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974406.1"
                     /translation="MSDLQKFAKFGIPVLLLLASFVTAAAQGDPDRQRAFQLYRDAKY
                     VEALPLFEQLALKYPEDPDVIENLGMLVFTQSAYLKTPEERKSARKRGRALLVQAQKL
                     GANSALLSAMIEGIPNDGGDDGSFSSKKEVDEAMHEGEAAFAKGDFPKAAEMYQRALM
                     LDPKLYEAALFTGDVYFKTADQAKASEWFARAVAINPDRETAYRYWGDSLMKQGKVTE
                     AGEKFVEAYIAEPYNRLARAGFVNWGDKIHIELAHPRVDLPANVAQKKPGEATLTIDP
                     SLFDKDKDKSGSAVAWVTYGLTRASWQEEFARQYPNEKTYRHSLKEEAAAMRSALKVL
                     GEQKGTDASKIDPSLQVIKKLDKEGLLEAFILLALPDAGIANDFTAYRKTNVEDLRRY
                     VKQYVMTGGGKQ"
     gene            complement(12654..13289)
                     /locus_tag="VFH96_10310"
     CDS             complement(12654..13289)
                     /locus_tag="VFH96_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974407.1"
                     /translation="MAEERTLGLARAQDLTTEEPSKEELQRRLGEARDSITNTVSEIK
                     ETVANQVQAVKDTLDWREQFKKRPVVWSAAALGAGFCTGYCIANYVKGDEHEYHSAID
                     RYAEETRHYATQAIASEGIAAPVHVKQGNGTHDTGPNFLQKLTNTQAFDRVKSEAGNI
                     GDAFVQELSKTAKTVVLPALITSLRNFIGDHLPNADTKKQSYQPALERNPT"
     gene            complement(13268..13720)
                     /locus_tag="VFH96_10315"
     CDS             complement(13268..13720)
                     /locus_tag="VFH96_10315"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6974408.1"
                     /translation="MAATKTSLTTNGLEVRNDAEGLPNLFSRLGDDVMQLFNSQLALF
                     KVEIKEEANAYARGITMIAIGAVIATIGFALLNVAIAFAVSTLFAQANFSQPASYALG
                     FVVTGGFYLLVGAIVVMLMKSRLARQDLVPQRTVQELRKDKQWLKNEL"
     gene            complement(13757..14092)
                     /locus_tag="VFH96_10320"
     CDS             complement(13757..14092)
                     /locus_tag="VFH96_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974409.1"
                     /translation="MPENGSINEPTTGVTGATPSTTGSTGTGTAAALSQTAHDYGQKI
                     SEVASQAKDYVSDKVSVVGDKFKELQNADLGELAENAKEYARQKPGQALLISAAAGLL
                     LGLIIRGRR"
     gene            14400..15056
                     /locus_tag="VFH96_10325"
     CDS             14400..15056
                     /locus_tag="VFH96_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6974410.1"
                     /translation="MTIEGAVPSARMAGEERRLQILAVAVSLFSQKGFRGTTTKEIAT
                     AAGVSEAMVFRHFATKQELYSAILDHKACASGRFDPAETAAVPMERKDDRAVFESLAL
                     GALDHHEKDPQFQRLLLYSALEKHELSQMFFDQIVRHVYEFLGGYIRERQRDGVFIDI
                     DPAVVVRCFIGMVMHHSLNNNLWDPDRRLLNISNESAAKVFTDILLKGITLPAATTEE
                     "
     gene            15072..16328
                     /locus_tag="VFH96_10330"
     CDS             15072..16328
                     /locus_tag="VFH96_10330"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6974411.1"
                     /translation="MQLTGKLKLALVMLLLVAVGAFVASCGGSKANVRKEANANAEPV
                     AVAVTTAAAIKRELPRFFEATGSLAGDQQTDVAPQTSGKVVAVGVDIGSYVRRGQMLV
                     QLDADELKLRVAQASAQLEQTKAAVRQAEEKIGLRSGQAFDPNRVADVAAAKVTLDLA
                     DKNLQRAEKLIESGDVSRSFYDDARARRDQLKEQYEVALARARQDYAGVDVARTNVAN
                     AQAQLGLAQKSLQYAVIPAPIDGFVTERVADLGEYVSPQQKVVTIVRTNPLRIRIDIP
                     EQAIQAVKVGQSVSISTSAWPDKNFAGRVARIAPNVTAQSRTLTVEAEIDNSNNALKP
                     GQFATVRILQERAEPAVLIPARAVVNDAGVNRVYVIKNGHAEQRLVQTGQTDGDLIEI
                     RNGIAADEQVATSNLEQLTDGIAIKQ"
     gene            16407..19598
                     /locus_tag="VFH96_10335"
     CDS             16407..19598
                     /locus_tag="VFH96_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6974412.1"
                     /translation="MQKLAEICVRRPVFATMLILSLTVVGLFSYKSLGVDLFPKIDLP
                     TITVTVVNPGASPQEIETEITDQVEGAVNTISGIDELRSTSVEGVSQVFITFLLEKNP
                     DVAAQEVRNKIELIINDLPVTAEAPIVQKLDTDAAPVVRIAVSAPRSLREVTDVADNQ
                     IKQQIESINGVGDVQIIGGRRREIQIWVDPDKMRAFNVTVAQVAEAVRAQNLEVPGGR
                     VDEGARELTVRTMGRIVQPAEFNDLIVANRGTYAVKLSDIGYAEDGAEEPRTEARLNG
                     QPAVTLIVSKQSGQNTVAVADAVKERLQEISRTLPPGFKTEVVGDQSIFIKASIEAIQ
                     THLIEGSILAAIVVFVFLWSFRSTVIAGLAIPTSLIATFGLMAAMGFTLNQITMLALT
                     LMVGIVIDDAIVVLENIFRFIEEKGVPPFQAAIEGTKEIGLAVMATTLSLLAVFLPVG
                     FMGGIVGRFMSSFGLTSSFAIAVSLLVSFTLTPMLAARLIKRSDDAKERALEKHTEPA
                     DHGSKESRFYRPIDRTYTRLLTWSMTHRWVVVVACVIVIVSIIPLFMFVGKNFLPVDD
                     QAQFELNVRAPEGYSLANTSTLAERIAADVRKLPAVTDTLVTIGGGLQEQVNVATIYV
                     KMTPIEERKISQQDLMVRARTEVLGKYQKLYPGQLRTSVQQVAAISGGGFRNADIQYV
                     LRGPDLNKLKQYSDAMLEKMKTIPDVVDVDSTLISGKPELRVEIDRARAADLGVRVGD
                     IAQALNSLVAGQKVSTFNAGTDQYDVRVRAVGEFRTSVEGLKRMIVSSTKVGWVSLDN
                     LVKVEEGTGPAAIDRLNRQRQVMLLGNVRPGGSQAAVIQKLDQFKKEINIDPAYQTGL
                     AGRSKELGRAGYYFLLAFVLSFVFMYMVLAAQFESFIHPITILLTLPLAIPFGILALL
                     MTGQTVNIFSGLGLLLLFGVVKKNAILQIDHTNQLRARGMERLEAIIRANRDRLRPIL
                     MTTIALVAGMLPLTISTGPGSGTNRSIGVLVVGGQTLCLLLTLLAVPVFYSLFDDLAR
                     SRVWGWSRDRVQGFFGRVRRRAATAAAGLISKQ"
     gene            19660..21309
                     /locus_tag="VFH96_10340"
     CDS             19660..21309
                     /locus_tag="VFH96_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6974413.1"
                     /translation="MVLGVASVVSAQETKPLPEMSRVGIDTNQAHPLSLREALAMALE
                     NNKDIEVARQNVRIAEFDYLGSQGAYDPRFTTQAFYERIKSPISSFLAGGQNGSTIQT
                     DYTGTARLEGQTPKFGGNYRLDFSSVKLNTNNQFVALNPQFPTSLTFSYTQPLWRGLK
                     IDQTRRQIQIARKNLALTDTQFRQRAIDTITNVQRAYWDLVFSLRNLQVQRDAVSTAR
                     SQLEHNKRLVNEGQLAPIDVVAAEAQISSFEQRVFGALEEVSRTENNLKNLIAQDQKA
                     NLWSESIVPTDSVDLAAPVVSLGDALKTAMENRPELQQADVLKEINQIDQKFFKDQTK
                     PAIDLVGTYGVTGLAGSVSSNIVNPLTQSSLLQRQRLDELSALAGLDPLPVIPPQQFS
                     QDLLGGYGQSLQNLLANRYGTARIGVQISLPLRNRTAEANLGRSLVEAERIGTQRKQL
                     EQTIQVDVRNALQSVRSSEARLRAAVATREANEQQFASEQRKLDAGQSTVFLVLQRQN
                     DLTESRGQELRAQTDLNKAIADLQRATGNALNVNSIVVRVR"
     gene            complement(21595..22455)
                     /locus_tag="VFH96_10345"
     CDS             complement(21595..22455)
                     /locus_tag="VFH96_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6974414.1"
                     /translation="MVLVLLLSLLVGHNDISLLESGQATTIRKQTSAPTEIKVITYNI
                     RWRSGDDLQTMIKLLQEDPEIGCASILALQEVDRHKKRTGHHNTVKTIADALGMYYAW
                     AAPPSVKSTDEEETGVAILSFYPLSDVHRIVLPHPGPGQRRRVALGATIEMPNGRWRI
                     YSAHAETRIKIDKKLEQYQAVLDDLAKFPADMPAMVMGDFNTWEPNVGGKVKKLFSNA
                     GWKTPFADQSTFRRKVLFLPIELKLDWVWLRGLEAAAFGIDKKVEVSDHFPLWTNVRM
                     EPQKSTKTQN"
BASE COUNT         4903 a         6089 c         6410 g         5111 t
ORIGIN      
        1 ctcggccgcc gccagtgatc agagctgttt tagccacgga tgacatcgat tgagaccgga
       61 ttagtgtccg gtaaatcgcg gttcgcgttt ttccaggaat gcgtgaacgc cttcgtgacc
      121 gtcgtcagat tcgaaacaac gcaactgcgc ttcggtctcg tagcgtagca tctcttcaag
      181 ctcagccgcc tggctcatgt agaccgcatg cttcgccgcc gcaagcgcga ttgggggcga
      241 cgctcgcaaa cgctcagcga gttgcagcgt tgtgctttcc aactcttccg gcgcaaccac
      301 ctgattgacg attccgagtc gcatcgcttc cgccgcatcg atcgactcgc cgagaaagaa
      361 catctcacac gctttgttcg gcgtcacgag tcgcggcaga aaatatgttc caccccagtc
      421 aggatgtagt ccgactttcg caaacgattg actgaacgtt gcattagtcg aggcgatgcg
      481 caggtcacac gcaagcgcga gattgcagcc cgcgcccgac gctggtccat tgatcgatgc
      541 aatgaccggt ttcgtcatct gtcgaatcgc gagaatcacg cgccggccgg cgccgagtat
      601 gcgcgagaat tcttccgcat cgcgacgctt catcaactca gccatgaacg cgatatcgcc
      661 gcccgcacaa aacccacgac ccgcgccggt aatgatcaca acgcgaacac cgcgatcgct
      721 gcccgcgtgt tcgagcgcct cagcgagatc gcgacgcatg tgaccgatga acgcattcaa
      781 ccggtccggg cgattcagca tgatcgtcag gatgccttca ttctctgcga cttgaatgtg
      841 ctcgtacatg ctagcgaccc ttgaattcag gcttgcgctt attcacgtaa gcgtcgagac
      901 cttccttcgc gtcttcagat tggaagagct gttgttgtaa ttcacgttcg agtgccagcg
      961 cggattcaaa cgggatctcg gaaccggttt gcacggcgcg cttgatgcga ccaaccgcgt
     1021 gcgcggcttt gtttggtgtc gtgaactggc gcgcgtaatc ctggaccatc tctgcgaaat
     1081 gttcaactgt ttcagcttcg aagatttgat tcacaatgcc gagcgcgaga ccacgctcga
     1141 agtcgaacaa ttgtccggtc gccatcatct cgatcgcttg cgatttgccg acgatccgca
     1201 cgagacgctg cgtgccgccc gttcccggga gtacgccgag cgagacttca ggcaatccca
     1261 ttttgcctgc gcctttgcgt gcgatgcgaa tgtcggcagc catcgcaact tcaagcccac
     1321 cgccaacgca gtgaccattg atcgctgcga tcacgagctt cggcgtttgc tccagacgat
     1381 tgagcgtctc gtttgcgtga agacagaaat agtatttata agccggcgtg acattcgcaa
     1441 gcatctggat gtcagcgcca gcgcagaaaa atttctcgcc tttacccgtg atcacgatca
     1501 cctgcacagc ctcatccatc cgcgcattga ggatggtgcg atcgagctct tgcatcatct
     1561 cgtagctgta ggtgtttgcg ggcggatcgt tgagcgtgat caaagcgatc ccgtccttca
     1621 cttcataatc gagttttagc atccgtgtta tccgtggctc caaacgctcg ggctttcaga
     1681 cggtagcgcc agactgctgc acataccatt cggcacaaac ccgttgagga ataatctcat
     1741 tacattgctc gccagatccg acacttttaa tttgccgtgc ggatcgtacc agttatagat
     1801 ccaattcatc atcccaaaga gcgcataagt cgcgaccgtc aagtcgatcg gctgctggct
     1861 ttgctgcgtc tcctggacct cacgcaggat ctgtcgcgcg agttgcgtgt atttgtcttt
     1921 gcgtgtcgag acgtgattat acagatcgcc gccaagcgac tcggcttcat gcgagagcac
     1981 tttcatctca gacatgttcg ccgcgaagaa tgaaaggtgg ttctcgatga agatgccgag
     2041 cttcgtgatc ggatctgcaa cgccttcgag gcgttgttct aatcgttcaa gcacacgacc
     2101 aaaacagttg tcttgaatga ggaataaaag ttcttctttc gacttgcagt agtgatacag
     2161 gcctgcgagg ctcacgttcg tcgcgcgcga gatgtcgcgc atcgacgtcg agtgataact
     2221 cttctcggca aagatgcgcg ccgccgtgcg cagtatgaat tcgtgttttt gatcgtatga
     2281 cttagccacg gatttacgcg gatttttgac ggattaaagc cttacatact cgtaatcaaa
     2341 tggcttgcct ttgatcccgg ctgcgggcgg cgcgatccaa tttgcgattt tgccgatttc
     2401 aaatacggga tgcatcaggc tgcgtacgta ctcgcgatct tctatcgtcg gtaaccactc
     2461 tgatttgcga ctcccaaact cttcacgcga gatcagattg ccctggagat caaaagtatg
     2521 accggcaaat tcaccgacgt gccggtgaaa gcgcgggtgc ggcagataca gacgctcgct
     2581 cgcaccttct tccgcgagca ctctgttcca acgtgcgagg cccttctcgc agtctttcac
     2641 atactcaccg cgcaacacct cattcaacgc attgcgtagc ggcacttcgc gagtcttcag
     2701 ttgaccatct tcaacaatcg tcatcgtgaa gttctcgttt gttgcggagt gatcggagta
     2761 ctgatcgcgc tccttgtaac gacccttcaa tcctgccgcg aagaagtcag cggagttgga
     2821 gctgatctcg ccgccgaaga gatcgagcga atagctgaac cagaaattga cgtacttctg
     2881 aatgatctcg agcggaatgc cgccaagctc gcgcacgtca ccttccttca tcagttgggc
     2941 ggtgcgacgg cagacgcggc ccagacccgt atcaccaaca aacatgtgat gcgcttcttc
     3001 agtcagcatg aactggcacg tgcgcgcgag cggctcgaag ccggattcgg cgagcgctga
     3061 gagctgatac ttgccgtcgc gatcggtgaa catcgtgaac gcgaagaagt tgagccagtc
     3121 gtcgcagggt tgattgaacg cttcgaggat gcgcggcttg tcaggattgc cgctgcgtcg
     3181 ctcgagtagc tcgtccgctt cgtcacggcc gtcgcgaccg aagtagccgt gtagcagata
     3241 gaccatggcc cacaggtgtc gtccttcttc gacgttcacc tgaaagagat tgcgcaggtc
     3301 gtaaagagag gggcaggtct tgccgagttg tcgttgttgt tcgactgacg ctggttccgt
     3361 gtcgccctgc gtgacgatga tgcgacgcag tgcattgcga aactcgccgg gcacttcctg
     3421 gtaagcaggt cggccgaggt tgtcgccgaa accgattttt gcatcgggct gcggtggctc
     3481 aagaaagatg ccccagcggt agtccggcat cttcacgtaa tcgaagtggg cccagccttc
     3541 ggagccgacg tcgacggcag tccggagcca cacatctttc tcctgaaaac cttcgggtcc
     3601 catctcacgc caccagtcga gatagtttgg cagccacttc tcgagcgcgc gctggagttt
     3661 cttgtcctcg gagagattaa cgttgttagg aatgcgatcg tataaattca tcattgcctc
     3721 aaaccatttt tagccacgaa aaggcacaaa aatcacaaaa gcgttgattg aattgagcgg
     3781 gctaacatca cttccatttt gtgacttttg tgccttttcg tggctaattc ttcttctgtg
     3841 ccttttgtgg ctacgttcgt ttgtaatcga agtgcgcctg tgttggtttg ccgtagttgg
     3901 tcagcgcgcc ttctggtcca acggcgttcg ggcgctggaa gatccagttt tgccacgcgg
     3961 ttagtcggcc gtaaattttt gtgtccatcg tttccgggcc ggcgaaacga agcgatgctt
     4021 ccatgccggt cagtgcgtcg ggtgataaag atgtgcgctc ttcgatggcg acgcgaatct
     4081 cgtcttccca atcgagatcg tcgggcgcaa aggtgatgag tccgagatct tcggcttctt
     4141 cagtgtcgaa ttttttcttg tcgccaaaca cttcatcgag cttctcgggc tcagcgagaa
     4201 agcgcgactg catgcgcgtc agaccgttgc tcatcagaaa cgcgccctga ttcaactccg
     4261 aaactgcaag cgatgcgtcc gagtccatga gcatgtaact gcgatcggaa gcgagcaaca
     4321 gctcgagcag gttgccggcg aaacaacttc ccggctcgac gatcgcaaag aaactcttcg
     4381 ccgtgagatc gagacgacga agcacgcgcg ccatgtgcaa aacgatttcg cgcacgagcc
     4441 agtgatcgtg attcgccgcc aacgtctgat cgatcgataa aacagtttca gcgtcgcctt
     4501 cggtgcggag gcagacgagg ccgatcaacg gttcatttac gcgcaggtgc agcagggcgt
     4561 tgtcgagctc gcggtaagct tgcagcggcc agtaagcagc gccgagctcg tgaatctgtt
     4621 cggcggtcgt gggctgcggg ccttcgggcg cgagaacggt cagatccgcg tagcgctgat
     4681 cgcgatttag tttgagtttg acgtacttgt agtttcgcgc gtcgtcagta gtctcaaact
     4741 cgagtggtgg tagcttgatt cctttagcag gcgccgcggc attcactaac tccttgacgc
     4801 gtgcatcgat cgactcctga aacttgctcg tcggaaagta gccgtcgatg agtccccact
     4861 ccttcgcgcg ttttcctttt aatccctctg cgagcgtcga gaacacatcc gcgcgatcgc
     4921 gtcgcacttt tcgtttgtcg acgagacgcg tgagcccacc tgtgcctggc aacacgccta
     4981 gaagcggaac ttcgggaagc gatacagcgg agttgccgtc gtcaacgaga tagatctcgt
     5041 cgcaagcaat tgcgagctca taaccgccgc ccgaggctgt gccgttcaag gcagcgatat
     5101 aacgtcgacc ggaatggctc gagtcgtctt caatcgcgca gcgcgtctcg ttagtgaact
     5161 tgcaaaagtt gactttgaac gcgtgtgacg acgagccgag catgtagata ttcgcgcccg
     5221 cgcagaagat tcgaggcttc aggctcgtga cgacgactgc ccgcacttcc ggatgctcga
     5281 aacggatccg ctggacagcg tcagccagct cgatatcgac gccgaggtcg tacgagttga
     5341 gcttgagctt gtagccgtcg gcgagcgtct cgtcttcctg gacgtccatg gagagcgtgg
     5401 cgacagggcc ttcgaccttc agcctccagt gtttgtattg ctcaggcgca gtttggaaca
     5461 gtatttcagg atttgacatg atttcaagat tctcagttcg aacgttcgtt cggattttag
     5521 gtgagactgt gctgtaggtc aaatgttgtc cacagatttc acagatgaac gaaaatctgc
     5581 gtaatctgtg gatgaacttg acagttttgc ctgacggcgc gtagggtttg gtgctttcag
     5641 acgcctggag gcgaatctgt tctggtgaac ggcgcggtct tcaaaaccgt tagtgagccc
     5701 gtgagagcgg gcttgggtag gttcgattcc tacacgcctc cgccagtaac cgaagtcggc
     5761 taaatcgttc ttttacaagg aatttcctca catgatcaga gattgggttg agctgaatat
     5821 gacaccaggc atcggtcctc gcgccgctgc caaactcctc gagcgtttcg gttctgccga
     5881 ggcagtctat aaagcaacca gagccgaatt ggagcagttg cgactgccgc cggaggccgt
     5941 cgataccatc atcgcccgcg acttgctccc tcgtgccgaa gccgagataa ctgcggtaaa
     6001 acaactcggc ggcgacattc tgttattaga cgacggggtt tatccctcat ccttacggga
     6061 aatctacgac ccgcctattg ttctgtatgt taaaggcgca tggtcagatt gtttggaccg
     6121 accctgtatc ggtgtggtag ggtcaagaag gtgttcgacc tacggccaga atgccgcaaa
     6181 catgctggcg cgcgacctag ccgagcgggg tgttaccgtg gtttcgggct ttgcacgtgg
     6241 catcgacgcc gctgcgcaca ggggtgcgct ggaggctaat ggccgcacag tcgctgttct
     6301 cgggacggga attgacgagg tttaccctcg tgagcacaag aagttagcgg ccgagatact
     6361 cgaccgcggc ggtgcgttcg tgagccagtt cccattgggg acgccaccgg tctcggaaaa
     6421 ctttccttac cgaaaccgca tcattagcgg gctgagtctt ggagtcgtgg tcgtggaagc
     6481 ggcggagaat tcgggatctt tgataaccgc gcggctggcg atggagcaga atcgggaagt
     6541 ttttgctgtg cccggaaata taacttctcg aaactcgttc ggcacgaact atctgataaa
     6601 aggcgcgggt gcgaagctcg tgcagcagtg gcaggatatc gccagtgagc tgccgcagcc
     6661 ggtagccgcg tcgcttttgc cgccgccgtt tagtgaacgg aagaatccgg tcagcgatcg
     6721 actggcgttc gttcccgagg gtctttcgcc ggcagagacg tgcgtgttta agttattgac
     6781 gccggacagc ccggcgcacg tagattggct ttttgataag agcaagctcc ccatttcgga
     6841 gctgacggct gcgctgctct ctttggaaat tcgagagttg ataagggcgc tgcccggtag
     6901 atgttttgtg agaaaactgt aaatggcaaa aaacctggta atagttgagt cgccggcaaa
     6961 ggcgaaaacc ataaacaagt atttaggaaa ggactatctg gtcaaggcct cgataggcca
     7021 tattaaagat ctgccttcca aaggtctcgg agttgatgtc gaacacaatt tcgaacccac
     7081 ttacgagctg attcccgatt cgaagaaacg taacaacaag aggatcgttg ccgaattaaa
     7141 gaaggctgcg aaagaggctg aagcaatcta tctcgccgcc gaccccgatc gagaaggtga
     7201 agcaatctgc cagcacctca aagaggagat cgttccgaag cgacctgcga agcccgcgtt
     7261 tcgcgtgatg ttcaatgaga tcacgaagcg cgcggtcaat gaggctttcg agcatccacg
     7321 acagatcgat gagaacctcg tcgatgcgca acaagcgcgt cgcgtgctcg atcgtttggt
     7381 gggttacaaa gtctcgccgt tgctgtgtcg cacgattggt gggcgattga gtgccggtcg
     7441 cgtgcagtca gtcgcgctca gaatggtggt agagcgtgag cgcgagatcg agaagtttgt
     7501 taagactgag tattggacca tcgctgcaaa tgtgagctcg aaactgccgc cggcgtttga
     7561 ttcacgactc ttcaagatcg acgagcagac agtcaagacc agcggcttcg atcaggatct
     7621 caagaagaat gagatcgtca tcaacgatca gaccaaggct gacgcgatcg tcgctgaagc
     7681 gcagcaacag cagttcgtcg tcactgacgt cacgacgaag gaacgtaaac gcaatccggt
     7741 tccaccgttc atcacttcaa aactacaaca ggaagcttcg cgcaagctcg gcttcgcagt
     7801 gaagaagacg atgatgctcg cgcaaaagct ttacgaaggc gtcgagctcg gtgacgaagg
     7861 ctctgtcggt ctcatcacct acatgcgaac tgactcgacg cgtgtttcgg atgcggcgct
     7921 gggtgaagtg cgcgattaca tcaacggtca atacggcggt aattacctgc ccgagaaggc
     7981 ggtccattat cgctcgaaga aagacgcgca ggacgcgcac gaagcgatcc gcccgacgga
     8041 agtcgcgcgc acgcccgatt cgctggcgag ctatctcaaa cccgaagagc tgaaactcta
     8101 tcgactgatc tggcagcgtt tcgttgcgtc gcagatgatg cctgctgtct tcgatcaaac
     8161 aacgatcgac attcaggccg gacgattcat cttccgtgcg accggttcgg tgcagaagtt
     8221 tgacggcttc ctcaagatct accaggaagg ccgcgacgaa aaggccgaag acgacgatga
     8281 agcagaacgc agtctaccgc tcgtcgaaaa aggcgagaac ctcaagctga acacgatcac
     8341 gcccgaacag catttcaccg agccgccgcc gcgttacacg gaagcgacgc tcgtgaaagc
     8401 gctcgaagag aagggcatcg gccggccttc gacttacgcc gcgatcatgt cgacgattca
     8461 ggagcgtgag tacgtcgaaa agctccaggg gcgctttcat ccaaccgcgc tcggcaagac
     8521 cgtcaacgat ctgctgatcg acggcggctt cgacgatctc ttcaacgaga cttacaccgc
     8581 gcggatggaa gaagagctcg acgacatcga ggaaggaaag attcgctgga ccaaagcgct
     8641 gcgtgagttt tacgacaagt ttgaaggcga cctgaaacag tttcagcatt acatcaaaga
     8701 catcaaggag aagaacgttc cgactgaaga ggtgtgtttg aagtgcaaca cgccgggcat
     8761 ggtccagaag tggggacgct ttggtccgta tctgaaatgt cttagctgcg aagctacgcg
     8821 tgatgctgag ccagtgaatg gttcgaacgg cgcatcggct tcaagcgagc cacaggcgaa
     8881 cggtgaagaa tcagccgagc cggaagtttg tgagttgtgt ggcaagccga tgcaattgaa
     8941 gcgcggacgt tttggtccgt tccttggctg cactggttat ccggagtgtc gcaacatcag
     9001 gaagatcggg aagagtggtg tggctgcacc tccgcccgta ccgattgatg agaagtgtcc
     9061 ggttgatggt gcgcaactgg tgaagcgtgt cgggcgtttc ggcgagttta tctcgtgctc
     9121 gaactatccg aagtgcaagt acatcaagca ggagacggtc ggagtttcgt gtccgcgtcc
     9181 gggctgcaaa ggcgagatcg tcgtgaagaa gtcgaaacgc ggcaaagcgt tttacggctg
     9241 ctcggaatat ccgaactgca atgtcgttta ctgggacaaa cccgtactcg agacttgccc
     9301 ggactgtaac gcgccattcc tgctcgaaaa gaccacaaag aagcaaggca ccttccgcta
     9361 ctgcgccaac gaagattgtc ctagccgcga aaaggaacaa aaaacacaaa aggtttctta
     9421 ggcacttatc cacagattac gcagattgtt ttatgacatc tgcggaatct gtggatgaac
     9481 tcattttgtg ccttttgtgc tttttgtggc agtattaggc gtccatccta tcttttaggt
     9541 acaacttcga ggagaccacc atgattcatc ggcgtattgc ggccgtagtg ctgctcctga
     9601 tattcatcat ctcgccggct tctttgcttt cacaacagac accggctcct acaccagatc
     9661 ctaacgatcc aattcaacgc gttaaggacg aaggcatgaa ccgctcgcag gtcatgcaaa
     9721 cgctgagcta tctcagcgac gtcattggac cacgcctgac tgcgtcacca ggcatgaagc
     9781 gggccaacga atggacgcgc gatcaactga ctaagtttgg gcttcagaat gcacatcttg
     9841 aagcctgggg accattcggt cgtggttgga ccttgaagcg gttttccgcg caagtgactg
     9901 acccgactgc gatccctctg atcgcgtatc cgaaagcgtg gtcgcccggt ctgtcgagcc
     9961 cgctcactgc tgacgttgtt tacttcgacg cgaagacaga agcagacttc gagaagttca
    10021 aaggcaagct caacggaaag atcgttttga cggcgccggt gcgcgacgtc agtgcgcatt
    10081 tcgaggcact cggcacgcgc ttgaccgaga aggatttgtt gacgctcgca gacgcgcccg
    10141 agccacgtgc cggcggcaat cgcggaaact ttgccgctaa cccgcaattc cgcgccgcgt
    10201 ttgagctggc gaatgcgaag atgcgcttct tccagtcgga aggcgcggct cttttgatcg
    10261 atccgagccg tggtgatggc ggtacgatgt tcgtgcagtc agctgcagtg ccgagtccgc
    10321 cgcgcgaccc gaacgcgacc acggcgaatc cattcgctcg cggcattccg ccatacgata
    10381 agagcgcgcc caaaaccatg ccgcaactcg tcgttgcgat cgagcactac aaccgcatcg
    10441 tgcgcatgct gcaggccggc gaggctgtga agatgaatgt tgatctgacg gttgcgtggc
    10501 aggacgccga tctcatgggc tacaacacga ttgccgagat tcccggcacc gatctcaaag
    10561 acgagatcgt gatgatcggt ggtcacatgg attcatggca ctcgggtact ggcgcgactg
    10621 ataacgccgc gggttgtgcc gtcgcgatgg aagccgtgcg cattattcag gctcttggta
    10681 ttaaaccgcg tcgcacgatc cggatcgctc tgtggagtgg cgaagagcag ggactactcg
    10741 gctcacgcgc ttacgttgcc gatcacttcg gtacgttgca aagcccggcg acgtccgccg
    10801 cgcccgcgac gggtggccta aacaatggca tgggcggcgg caatggaaac ggtgctccgg
    10861 ctgcaccaac gctcatcacg aagcccgagt atgagaagct gagtgcttac tacaatctcg
    10921 ataacggcac gggcaagatt cgcggcgttt acctgcaggg caacgaagcc gtgcgctcgc
    10981 tgttccgaca gtggctcgcg ccattccggg acctcggcgc caccacgctc tcgatcgcga
    11041 acaccggcgg tacggaccac ctctcgtttg atgggattgg tttgccgggc ttccagttta
    11101 tccaggacga gattgagtac gacacgcgca cacaccactc aaaccaggat gtgttcgatc
    11161 gtattcaagg ggacgacatg aagcaggcag cgacgatcat ggccgcgttt ctttaccaga
    11221 cggcgatgcg tgacgataag atcccacgca aacctgctcc tggagcgaga taaaatctgc
    11281 gaaatctgtg taatctgtgg ttagctatcc gcagattaca cagattgtta ttgctttccg
    11341 ccgccggtca tcacgtactg cttcacgtag cggcgcagat cttcgacgtt ggttttacgg
    11401 taggcggtga agtcgttggc gattccggcg tcgggaagcg cgagcagaat aaaagcctcc
    11461 aacaatcctt ctttatcgag cttcttgatc acttgtagcg atgggtcgat cttactcgcg
    11521 tcggtccctt tctgttcacc cagcactttc agagcagatc tcatcgccgc ggcttcttct
    11581 ttcaaactgt gacgatacgt cttctcattc ggatactgcc tggcaaactc ttcctgccaa
    11641 ctagcgcgag tcaatccata cgttacccac gcgaccgctg aaccactctt gtccttatcc
    11701 ttatcgaaca aactcggatc gatagtcagc gtcgcttcac ctggcttctt ctgcgcgacg
    11761 ttcgcaggaa gatcgacacg cgggtgggcg agctcgatgt ggattttgtc tccccagttc
    11821 acaaagcctg cgcgggcgag acggttgtaa ggctcggcga tgtaagcttc gacaaacttc
    11881 tcacctgctt cagttacttt gccctgcttc atgagcgagt cgccccagta gcggtaagca
    11941 gtctcacgat ccggattgat agcgactgct cgcgcgaacc attcactcgc tttcgcctgg
    12001 tcggctgtct tgaagtagac gtcgccagtg aagagtgcgg cttcgtagag tttcggatcg
    12061 agcatcagcg cgcgctgata catctcggct gccttcggaa aatctccctt ggcgaacgcg
    12121 gcttcacctt cgtgcattgc ctcgtccact tctttcttgg acgagaaact cccgtcgtct
    12181 ccgccgtcgt tcggaatacc ttcgatcatg gcactcaaga gcgcgctgtt tgctccgagt
    12241 ttttgtgctt gcacgagcag tgcgcggcct cgtttgcgag ctgacttacg ttcttcggga
    12301 gtcttcaggt aagcgctctg cgtgaacacg agcattccga gattttcgat gacgtcagga
    12361 tcttccgggt atttgagcgc gagctgctcg aagagcggca aagcctcaac gtactttgcg
    12421 tctcggtaaa gttgaaaagc gcgttggcga tcgggatctc cctgcgctgc ggcggttacg
    12481 aacgaggcga gaagcaggag gaccggaatt ccgaattttg caaatttttg gaggtcggac
    12541 atgcgggaga gtctatcccg ctgcgatgaa aaagcaaagt ttaagccgcg gatttacgcg
    12601 gataaacacg gatctaaatc ggcgttcatc cgcgaagatc cgcggctaaa aatttaggtc
    12661 gggttacgtt ccaacgcggg ttggtaactt tgtttttttg tatccgcgtt cggaagatga
    12721 tccccgatga agtttcgcag cgaagtgatc aacgctggta gtaccaccgt ctttgcggtc
    12781 ttcgaaagct cctgcacaaa agcatcgcca atatttcctg cctcactttt cacgcggtca
    12841 aaggcctgtg tattggttag cttctgtaag aagtttggtc cagtgtcgtg cgtaccgttt
    12901 ccctgtttca cgtgcacggg agccgctatc ccttcactcg cgattgcctg tgtcgcgtag
    12961 tgccgcgttt cctcggcgta gcgatcgatc gccgagtggt actcatgttc gtcgcctttt
    13021 acgtagttgg cgatgcagta accggtacag aagccggcgc ccagggccgc cgcgctccaa
    13081 accaccggcc gtttcttaaa ctgttcacgc caatcgagag tgtctttaac tgcctgtact
    13141 tgattcgcca cagtttcttt tatctccgat actgtatttg tgatcgaatc gcgcgcttca
    13201 cccaaacggc gctgaagttc ttccttggaa ggctcctcag tcgttaggtc ttgcgcgcga
    13261 gccagtccta gagttcgttc ttcagccatt gcttatcctt tctcagttcc tgaactgtcc
    13321 tttggggcac cagatcctgc ctggcgagcc ggctcttcat gagcatcacg acgatcgcac
    13381 ccaccagcag gtagaagccg ccagtcacta caaacccgag agcgtaactg gccggttggc
    13441 tgaaatttgc ctgcgcgaac agagtcgaga ccgcgaaagc gatcgctaca ttgagaagtg
    13501 caaagccaat ggtggcgatc acggcgccaa tcgcgatcat ggtgatgccc cgcgcgtagg
    13561 cgtttgcctc ctccttgatc tccactttga ataacgcaag ctgagaattg aagagctgca
    13621 ttacgtcgtc gccgagacgg ctgaaaaggt ttggaagacc ttcagcgtcg ttgcgtactt
    13681 ccagaccgtt cgtagtcaga cttgtttttg ttgccgccat gtttggttca ccccctgttc
    13741 ggtactcgcg accggcttag cgccggcccc gaataatcag accgagtaat agacccgctg
    13801 ctgctgaaat cagaagtgcc tggcctggtt tctggcgcgc atattctttc gcgttctctg
    13861 cgagctcgcc gagatctgcg ttttgcagtt ctttgaattt gtcgccgacg accgagactt
    13921 tgtcactcac gtaatctttc gcttgtgaag cgacttcgga gatcttctga ccgtagtcat
    13981 gagctgtctg cgatagagcg gccgcggtgc cggttcctgt gcttccggtc gtacttggag
    14041 ttgcgcctgt tactccggtg gttggttcgt tgatgcttcc attttctggc atgtcgaaat
    14101 actccttctc agatgaaaac tagaatttgg gctagccgca cagtggagca agctttgtgc
    14161 cgatagaatg tgagggaaag aatggaattt ctgcgcgtat cttgttgcga aagaatacaa
    14221 cggtgcataa atccgtccac tatccgtcta aatccgcggt taattagcca cggataacgc
    14281 ggatcgaaaa cggatttaac caactccact taacttcttg acatcaaagc gcgtcagatg
    14341 gtaagtaagt acttacttta cgccaggaag aaagaagaca aataggattg agtaagcaga
    14401 tgacgattga aggggccgtg ccgagtgccc ggatggcggg agaagagcgg cggctccaga
    14461 ttctcgcagt ggccgtaagc ctgttctcac agaaaggttt tcgcggcacc actacaaaag
    14521 agattgccac cgcagccgga gtctccgagg cgatggtgtt tcgtcacttc gccaccaaac
    14581 aggagctcta ctccgccatt ctcgatcaca aagcctgcgc cagtggtcgc tttgatccgg
    14641 cagagacggc tgcggtgccg atggagcgca aagacgatcg cgccgtcttc gagtcgctcg
    14701 cactcggcgc cctggatcat cacgaaaagg atccgcagtt tcagcgactg ctgctctatt
    14761 cggcactcga gaagcacgag ctctcgcaga tgttcttcga tcagatcgtg cggcatgttt
    14821 atgagtttct gggcggctac attcgtgaga gacagcgcga cggagtgttc atcgacatcg
    14881 atccggcggt cgtggtgcgc tgctttatcg gcatggtgat gcaccactcg ctcaacaaca
    14941 atctctggga tcccgatcgt cgtttgctga acatctcgaa cgagagtgcg gcaaaggttt
    15001 tcacagacat tctcttaaag ggaatcactt tgcccgcagc aacgactgaa gaatagttaa
    15061 ataaagacaa gatgcaactt actgggaaat taaaactcgc gcttgtaatg ctgctgctcg
    15121 tcgccgtcgg agcttttgtc gcgtcgtgtg gtggctcgaa agcgaatgtt cgaaaagaag
    15181 cgaacgcgaa tgcggagccg gtcgctgtcg cggtgacgac tgcggccgcg atcaaacgcg
    15241 agctgccgcg cttctttgaa gcaaccggca gtcttgcagg cgaccagcaa actgacgtgg
    15301 cgccgcaaac ttcaggcaag gttgtcgctg tcggcgtcga catcggcagc tacgtgaggc
    15361 gcggtcagat gctcgtgcag cttgacgctg acgagttgaa actgcgtgtt gcgcaggcgt
    15421 ccgcgcaact cgaacagacc aaagccgcgg ttcgccaggc agaagagaag atcgggcttc
    15481 gttcggggca ggcttttgat cccaatcggg ttgcggacgt cgctgccgca aaagtcacgc
    15541 ttgatcttgc cgacaagaat ttgcagcgcg cggagaagtt gattgagtca ggcgacgtgt
    15601 cgcgttcgtt ttacgacgac gcgcgcgcgc gtcgcgatca gttgaaagag cagtatgaag
    15661 tcgccctcgc acgtgctcga caggactatg cgggagtcga cgtggcgcgc acgaatgtcg
    15721 cgaacgcgca ggcgcagctc gggctcgcgc aaaagagttt acagtacgcg gtcattcccg
    15781 cgccgatcga cggttttgtg actgaacgag tagctgacct gggcgaatac gtttcgccgc
    15841 agcagaaagt cgtgacgatt gttcgcacga acccgttgcg cattcgtatc gacattcccg
    15901 aacaggcgat tcaggcagtg aaggtgggcc agtcagtatc gatctcgacg agcgcatggc
    15961 ctgacaagaa cttcgccggg cgtgtcgcgc gcatcgcgcc gaatgttacc gcgcagtcac
    16021 gcacgttgac tgtcgaagcg gagatcgata acagcaacaa cgcgctcaag ccgggccagt
    16081 ttgccaccgt gcgaattttg caggagcgtg ctgagcctgc agtgttgatt ccggcgcgcg
    16141 cggtcgtgaa cgacgcgggt gtgaatcgcg tgtacgtgat caagaacggc cacgccgaac
    16201 agcgtctcgt gcagacaggt caaacagatg gcgacctcat tgagatcagg aacggtatcg
    16261 ccgcggacga gcaggttgcg acgagcaatc tcgaacagtt gactgacggc atcgctataa
    16321 aacagtagta cacaaaaggc acaaacagga gtcacaaaaa ggcacaaaag acatcctgta
    16381 aatcttggaa atcttgtaat cctgttatgc agaagcttgc tgaaatttgt gttcgccgtc
    16441 cggtcttcgc gacgatgctg attctcagcc tgacggtcgt cgggctgttc tcctacaagt
    16501 cgctgggcgt cgatctgttt ccgaagatcg acttgccgac gatcactgtc acggtcgtga
    16561 atcccggcgc gtcgccgcag gagatcgaga ctgagatcac cgatcaggtg gaaggcgctg
    16621 tcaacacgat ctccggtatc gacgagctcc gctcaacgtc cgtcgaaggt gtttcgcaag
    16681 tcttcatcac gttcctgctc gaaaaaaatc ccgacgtcgc ggcacaggaa gtcaggaaca
    16741 agattgagct gatcatcaac gatcttccgg tcacagccga agcgccgatc gtacaaaaac
    16801 tcgatactga cgcagcgccc gtggttcgca tcgcggtgtc cgcgccacgc tctcttcgcg
    16861 aagttacaga cgttgccgac aaccagatca agcagcagat cgaatcgatc aacggcgtcg
    16921 gcgacgtgca gatcatcggc ggacgccgtc gtgaaattca gatctgggtc gacccggaca
    16981 agatgcgcgc gttcaacgtg acagtggcgc aggtcgcaga agcagttcgc gcgcaaaacc
    17041 tcgaagttcc cggcggtcgt gttgacgaag gcgcacgcga gctgacggtg cgcacgatgg
    17101 gtcgcatcgt ccagcccgcc gagttcaacg atctcatcgt cgcgaatcgc ggcacttacg
    17161 cagtcaaact cagcgacatc ggttacgcgg aagacggagc cgaagagccg cgtactgagg
    17221 cgcgactgaa cggccagccc gccgtgacgc tgatcgtctc gaaacagtcg ggccagaaca
    17281 cggttgccgt cgctgacgca gtgaaagagc gtttacagga gatcagcagg acgctgccgc
    17341 cgggcttcaa aaccgaagtc gtcggcgacc agtcgatctt tatcaaagcg tcgatcgaag
    17401 cgattcagac tcacttgatc gagggcagta ttctcgccgc gatcgtcgtg ttcgttttcc
    17461 tttggagttt ccgatcgaca gtcatcgccg gtctcgcaat tccgacgtcg cttatcgcca
    17521 cttttggact gatggcagcg atgggcttca cgttgaatca aatcacgatg ctcgccctga
    17581 cgctgatggt cggtatcgtg atcgacgacg cgatcgtcgt actcgaaaac attttccggt
    17641 ttatcgaaga gaaaggtgtg ccgccgttcc aggccgcgat tgaaggcacg aaggagatcg
    17701 gcctcgcggt catggcgacg acgttgtcgc tgctcgccgt gttcctgccg gtcggtttca
    17761 tgggcggcat cgtcggacgc ttcatgtcgt cattcggttt gacgtcgtcg ttcgcgattg
    17821 cggtttcgtt gctcgtttcg ttcacgctca cgccgatgct tgcggcgcgt ttgattaagc
    17881 gtagtgacga cgcgaaagag cgtgcgctcg agaaacacac cgagcccgcg gatcacggct
    17941 cgaaggaatc acgtttttat cgtccgatcg atcgcacgta cacgaggctg ttgacgtggt
    18001 ccatgacgca tcgctgggtc gtcgtcgtcg cttgcgtgat cgtgatcgtg agcatcattc
    18061 cgcttttcat gttcgtcggt aagaactttc tgccggttga tgaccaggcg cagtttgagc
    18121 tcaatgtgcg cgcgcccgaa ggttattcgc tggccaacac ctcgacgctc gccgaacgta
    18181 tcgctgccga tgtgcgcaag ctgcccgcag tgaccgatac cctcgtgacg atcggcggcg
    18241 gcctgcagga acaggtaaac gtcgccacca tctacgtaaa gatgactccg atcgaggagc
    18301 gcaagatttc gcagcaggat ctgatggtgc gtgcgcgcac tgaggttctc ggtaaatatc
    18361 aaaaactcta tcccggacaa ctacgcacga gcgtgcagca ggtggctgcc atctccggcg
    18421 gcggattccg caacgctgac attcaatacg tgttgcgtgg tccggatctc aacaagttga
    18481 agcagtactc cgacgcgatg ctggaaaaga tgaagacgat tccagacgtc gtcgacgttg
    18541 actcgacctt gattagcggc aagccggaac tccgcgtcga gatcgaccgc gcccgcgcgg
    18601 cggatctcgg cgtgagagtc ggcgacatcg cacaggcgct caactcgctt gttgcgggtc
    18661 agaaggtgtc gacgttcaac gcgggaactg atcagtacga cgtgcgcgtg cgtgcagtcg
    18721 gagagtttcg cacgagtgtc gaaggcttga agcgaatgat cgtgtcgtcg acgaaggttg
    18781 gctgggtcag tctcgacaat ctcgtcaaag tcgaggaggg aaccggaccg gcggcgatcg
    18841 atcgtctgaa tcgacaacgg caggtgatgt tgctcggcaa cgtgcggccc ggtggttcgc
    18901 aggctgcggt gatccagaag ttggaccagt ttaagaaaga gatcaacatc gatccggctt
    18961 atcagaccgg tctggcggga cgttcgaaag agcttggccg cgcgggttat tacttcttgc
    19021 tggcgttcgt gctttcgttt gtcttcatgt acatggtgct cgcggcgcag tttgagtcgt
    19081 tcattcaccc gatcacgatt ttgttgacgc tgccgctcgc gattccgttc ggcattctgg
    19141 cgctgctgat gacgggacag acggtgaaca tcttctcggg gcttggattg ttactgttgt
    19201 ttggtgtggt caagaagaac gccatcttgc agatcgatca cacgaatcaa ctgcgcgcgc
    19261 gtggcatgga acggctcgaa gcgatcattc gcgcgaaccg agatcgcttg cgtccgattc
    19321 tgatgacgac gatcgcgctc gtggcgggca tgttgccgtt gacgatctcg actggtccag
    19381 gttcgggaac gaatcgatcg atcggcgtgc tggtggttgg cgggcagacg ctttgtttgt
    19441 tgctgacgtt attggcggtg ccggttttct attcgctgtt tgacgacctc gcgcgcagtc
    19501 gtgtttgggg ttggagtcgg gatcgcgtgc agggcttttt tggacgggtg cggcggaggg
    19561 cagcgacggc tgcggctggg ttgatctcaa agcagtaaca cggattaagc ggattttgta
    19621 aaacggatta acacggatat gagaaagttt gcgttattaa tggtactggg tgtggcttcg
    19681 gttgtgagcg cgcaggaaac gaagccgctg ccggagatga gtcgggttgg tatcgatacg
    19741 aaccaggcgc acccgctgtc gttgcgtgaa gcgttggcga tggcgctcga gaataataaa
    19801 gacatcgaag tggcgcgaca gaatgtgcgg atcgcggagt ttgattacct cggctcgcag
    19861 ggcgcttacg atccgcgctt cacgacgcag gctttttacg agcgcatcaa gagcccgatt
    19921 tcgagcttcc tcgccggcgg ccagaacggc tccacgattc agaccgatta caccggcacc
    19981 gcgcgtctcg aaggacaaac accgaaattc ggcggcaatt atcgtctgga cttctcgtcg
    20041 gtaaagctca acacgaacaa ccagttcgta gcgctcaatc cgcagttccc aacgtcgctg
    20101 acgttcagtt acacgcaacc gctctggcgc gggctcaaga tcgatcagac gcgccggcag
    20161 attcaaatcg cgcgcaagaa ccttgcctta actgatacac agtttcgcca gcgcgccatc
    20221 gacacgatca caaacgtgca gcgtgcttac tgggatctcg tattttccct tcgcaatctg
    20281 caagtgcagc gtgacgccgt ctcgactgca cgctcgcagc tcgagcacaa caagcgtctg
    20341 gtgaacgaag gacaactggc accaatcgac gtcgtcgctg ctgaagcgca gatctcatct
    20401 ttcgagcaac gcgtttttgg tgcgctcgaa gaagtgtcgc gtacagagaa caatctcaag
    20461 aatctgattg ctcaggatca gaaagcgaat ctctggtctg aatcgatcgt cccgactgat
    20521 tcagtcgatc tcgcagcgcc cgtcgtgtct cttggcgacg ctttgaagac ggcgatggag
    20581 aaccggccgg agttgcaaca ggcagacgtg ttgaaggaga tcaatcagat cgatcaaaag
    20641 tttttcaagg accagaccaa gcccgccatc gatctggttg ggacttacgg tgtaaccggt
    20701 ctcgccggtt ctgtcagcag caacatcgtg aatccgttga ctcaatcgag cctattacaa
    20761 cgacaacgcc ttgatgagct ttctgcactc gctggtctcg atccgctgcc ggtaattccg
    20821 ccgcagcagt tctcgcagga cttactcggc ggctacgggc agtcgttaca aaacctcctg
    20881 gcgaatcgct acggcaccgc cagaatcgga gtgcagatca gtttgccttt gcgtaatcgc
    20941 actgctgaag caaacctggg ccgctccctc gttgaagccg agcgcatcgg aacgcagcgc
    21001 aaacagctcg agcaaacaat tcaggttgat gttcgtaatg cgttgcagtc ggtgcgaagt
    21061 tctgaagctc gtttgcgtgc ggcggttgcg acgcgtgaag ccaacgaaca gcagtttgcg
    21121 agtgagcaga ggaaattaga cgcgggtcag tccacagtgt ttctcgtgct gcagagacaa
    21181 aacgatttga ctgaatcacg cgggcaggag ttgagggcgc aaacagatct gaataaggcg
    21241 attgcggatt tacagcgagc cacgggcaat gcgttgaatg tgaacagtat cgtcgtgcga
    21301 gtgcgctaac aggtttcccc gtgcggctgc accaaaagtc acgacgtagc ggtgagtttc
    21361 agggggcgaa actcaccgct tgccttttta aagaatggct tcgacgaatt tgaacgcgac
    21421 cacgatcgct accacaccac cgatcgaagc aattaaatac cacgccggct gcttgtatcg
    21481 acaagctgga caacgggttc gtgtcggggc cacgtccctt tcgcaattac gacacaacat
    21541 ttgagatcac tcttcgccgc taagacacaa gctacacaaa aatgaattgg gggttcagtt
    21601 ttgtgtcttc gtgctttttt gtggctccat tctaacattg gtccacaagg gaaagtgatc
    21661 cgaaacttca acttttttat cgattccaaa ggcagcagct tccagtcctc gcagccaaac
    21721 ccagtcaagc ttcagctcga tggggagaaa aagaactttt cgtctaaacg tcgattgatc
    21781 tgcaaacgga gttttccatc cggcgttcga aaaaagtttt ttgactttgc caccgacgtt
    21841 cggctcccag gtattgaagt cgcccatgac catcgccggc atgtcagccg gaaacttagc
    21901 gaggtcgtcg agcacggctt gatattgctc caacttttta tcgattttaa ttcgggtttc
    21961 cgcgtgcgct gaataaatac gccagcggcc attgggcatt tcgatagtag cgccaagcgc
    22021 gacgcggcgc cgctggcctg gtccaggatg cgggaggacg atgcgatgca cgtctgacag
    22081 cgggtaaaaa ctcaaaatcg caacgccggt ttcctcttcg tcagttgact tgactgacgg
    22141 tggcgcagcc caggcgtagt acatacccaa cgcgtcggcg atcgtcttta ctgtattgtg
    22201 atgtccggtg cgtttcttgt gtcgatcgac ttcctgtagc gcgagtatcg aagcgcaacc
    22261 gatctcagga tcttcttgta gcaacttgat catggtctga agatcgtcgc cgctgcgcca
    22321 gcggatgttg taagtgatga ctttgatttc agttggcgcg gaagtttgtt tccggatggt
    22381 cgtggcttgt cccgactcga ggagggaaat gtcgttgtgg cccacgagta gagaaagcaa
    22441 gagtacgagt accatcatat ctccaatctt tcgtggttag tttccggtcg tcattttaag
    22501 ctaaactccg tcc
//
LOCUS       DASSFQ010000284         5129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_284867, whole genome shotgun sequence.
ACCESSION   DASSFQ010000284 DASSFQ010000000
VERSION     DASSFQ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284867"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFH96_10350"
     CDS             <1..917
                     /locus_tag="VFH96_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974415.1"
                     /translation="TVTWYLKEAIKTKKEKRQEAEREADRKKEPVGWPTREQLRAEEE
                     EEAPAIIVTITDSSDNVVKRLTGPVGAGVQRLTWDLRYPAANLSAPPPPDADPDFEPP
                     SGPIVMPGSYKAVVAKKVDGVVTQLGPPQQFQVTVEGQENMSSADHTALVEFQAKAMR
                     LQRAASGATAAANQLRPRLAAIKRAIAETPSLPQKLQEDATALDRRTSEILRALSGDN
                     TARQRNMNTPPSINDRIGYVVGAQRMSISRPTQTQIAQYNAAAQDFETTLAQLRQLIE
                     VDLARLEKQLEAAGAPWTPGRIPDWKNQ"
     gene            955..3417
                     /locus_tag="VFH96_10355"
     CDS             955..3417
                     /locus_tag="VFH96_10355"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET6974416.1"
                     /translation="MKRFGLLLFVIICFLPIHAQKPQTLSEKVAGMEKFPGFFPFYWD
                     AKTGKIWLEIDKWNTEFLYVESLPAGIGSNDIGLDRGQLGASSIVRFDRVGPRVLLVA
                     PNYDFRALTNNPDERLAVRDAFAESTLWGFEVAAEDGNRVLVDATSFYLRDVHQVTNT
                     LQRNQQGAYRLDPTRSVFYLPNTKNFPQNTEVETTLTFTGEPAGQFVRQVVPVPEAIT
                     VRERHSFVQLPPPGYKPRVFDPRASYAGIDYMDFATPIEEPITKRYISRHRLQKKDPS
                     ASASEAVKPIVYYVDRGAPEPVRSALIEGARWWNQAFEAIGYKDAFRVEVMPPDVDPM
                     DVRYNVIQWVHRSTRGWSYGSSLTDPRTGEIIQGRVSLGSLRDRQDFLIAEGLLAPYD
                     KTNTASPKLLEMVLARLRQLSAHEVGHTLGLQHNYAASPAGRSSVMDYPAPRAKLGAD
                     GVPDLSDAYAKGIGEWDKVSIAYGYQDFPPGTNEAAALDRTLSDAFKRGLMYLTDQDA
                     RPPSAASSVAHLWDNGANVIDELGNVMKVRAAALRRFGENNIREGAPMATIEDVLVPI
                     YMYHRYQVEAAAKVIGGQDYTFSIKGKGDRNPQIVPAEEQRRALAAVLDTIKPEALAV
                     PESLLRLIPPRPAGFQRTREDFRIRTQPVFDALAPAEAIADHVSGFLLNSERAARLIQ
                     FHARDQRNPSLAEVIDKIFAATWKAPAATGYAGEIQHSVNFIILSDLMALASAERASN
                     QARAIASWKIEQLKAWLTAQSRFPVDENQRAFMFYSLEQIKRFQEDPKKMNLTPAQQP
                     PDGQPIGMDWWSRADEDCSYRN"
     gene            3485..>5129
                     /locus_tag="VFH96_10360"
     CDS             3485..>5129
                     /locus_tag="VFH96_10360"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6974417.1"
                     /translation="MPEAEEYVGEVLDDKYRLEHLLGKGGMGAVYLATHLGTERYVAL
                     KLISPQFMRNKEFVERFKREARAAGRLRHPNVVDVTDFGFARVEAGQVAYLVMEYLDG
                     CTLGDVLAEEKRLPLEWVADILDQVCSAVHEAHQQGIVHRDLKPDNIWLEPNRLGSYR
                     VKVLDFGIAKVGEVAPPALDEDPAATIIIDDHSAPNGAQLESDTLMRPVGDESLTQQF
                     RIKGDGEGVGTLLQDPLTNPLLSKSDHVADDDGTLLFTDPGTHSAARRTAHIATSPQE
                     GAALTRVGAIMGTPLYMSPEQCGGGYVDTRSDIYSLGVIAYQMLAGEPPFAGNTSAVM
                     RAHREQIPTHLREREAKIPKRAAGVVMSALSKNPAERPQTAFAFGSAMRAQSEGVGTL
                     YRRAFSLYSEYFPKFLKLSLIAHVPMIVTSILLVSAFVWYKYSPPVGILHKVIVYCAI
                     AILSLMKFVATTIAVGVISGVTAIIITQLTVAPLRPVKLREAFAVMRKRWRPFVKTLV
                     RVTLRIFIGLILLVVPGMIMWMRYSLYAPVVLMEGLEGKA"
BASE COUNT         1154 a         1460 c         1467 g         1048 t
ORIGIN      
        1 ccacggtcac atggtatctc aaggaagcca tcaagacgaa gaaagaaaag cggcaggaag
       61 ccgagcgtga ggctgacaga aagaaggagc cggtcggttg gcccacgcgt gagcaactac
      121 gcgctgaaga ggaagaagaa gcgcccgcta tcatcgttac catcactgac tcttccgaca
      181 atgtcgtgaa acggctgact ggaccagttg gcgccggcgt tcagcgattg acgtgggatc
      241 tgcgttatcc ggccgcgaac ctgtccgcgc cgccaccgcc ggatgccgat ccggacttcg
      301 aaccgccgtc tggtccaatt gtcatgccgg gcagttacaa agcggtcgtc gcgaagaaag
      361 ttgacggcgt agtcacgcaa cttggtccac cacagcagtt tcaggtaact gtcgaaggcc
      421 aggaaaacat gtcgagcgcg gatcacacag cgctcgtcga gttccaggcg aaagccatgc
      481 gcctgcaacg cgcagcgagt ggcgccactg ccgcggctaa tcaactgcgg ccacgtctcg
      541 ccgcgatcaa acgagcgatt gccgaaacac cctcgttgcc gcaaaaactt caggaagatg
      601 cgacggcact cgatcgacgc acaagcgaga tcctgcgggc cttgagcggt gacaacacgg
      661 cgcggcaacg gaatatgaac acgccgccgt cgatcaacga tcgcatcggt tacgtcgtcg
      721 gcgcgcagcg aatgtcgatc tcgcgtccga cgcaaaccca gattgcgcaa tacaacgcgg
      781 ctgctcaaga cttcgaaacg acgctcgctc agttgcgaca attgatcgaa gtcgatctgg
      841 cgcgactgga gaaacaactc gaagccgccg gtgctccatg gacaccaggc cgcattccag
      901 actggaaaaa tcagtaataa atccgcgttc atccgtggct aaaaggagct aagcatgaag
      961 cgcttcggtc tcttgctgtt cgtaataatc tgctttttgc cgatccatgc ccagaaaccg
     1021 caaaccctct ccgagaaagt cgcgggcatg gaaaagtttc ccggcttctt cccgttctac
     1081 tgggacgcaa agacaggaaa gatctggctc gagatcgaca agtggaacac cgagtttctc
     1141 tacgttgagt cactgcccgc cggaatcgga tctaacgaca tcggtctcga tcgaggccaa
     1201 cttggagcta gctccatcgt ccgcttcgat cgggttggac cacgcgtatt gctggtggcg
     1261 cccaactacg atttccgcgc cctcaccaac aatcccgacg aacgtctggc ggtgcgagat
     1321 gcatttgcag aatccacgtt gtggggattc gaagtcgccg ccgaagacgg caatcgcgtg
     1381 ctggtcgatg caaccagctt ctacctgcgc gacgtccatc aagtcacgaa cactttgcaa
     1441 cgcaaccagc agggcgctta tcggctcgat ccgactcgca gtgttttcta tcttcccaac
     1501 acaaaaaact tcccgcaaaa cacagaagtc gaaaccacgc tcacgtttac cggcgaaccc
     1561 gcaggtcagt tcgtacgaca agtggtgccg gtaccggaag cgatcacggt gcgcgagcgg
     1621 cactcgtttg tgcagttgcc gccgccgggc tacaaaccac gtgtcttcga tccgcgcgca
     1681 agttatgccg gcatcgacta catggacttc gctacgccga tcgaagagcc gatcaccaaa
     1741 cgctacatct cgcgtcatcg tttgcagaag aaagatccga gtgcgagcgc cagcgaagcg
     1801 gtgaagccga tcgtttacta cgtggatcgc ggcgcgcctg aaccggtgcg ttcggctttg
     1861 atcgaaggcg cgcgctggtg gaatcaggcg tttgaagcga ttggttacaa ggatgcattt
     1921 cgtgttgaag tgatgccacc ggatgttgat ccgatggacg tccgctacaa cgtcattcaa
     1981 tgggtgcatc gctcgacgcg cggttggtcg tatggaagtt cgcttaccga tcctcgcacc
     2041 ggcgagatca ttcagggacg cgtgtcactc ggctcgcttc gcgatcgcca ggactttctg
     2101 atcgccgaag gcttgctggc gccctacgac aagacgaaca ccgcatcacc aaaattgttg
     2161 gagatggtcc tcgcccgttt gcggcaacta tctgcgcacg aggtgggcca cacgttgggt
     2221 ctgcagcata actacgcggc gagtccggcc ggtcgttcgt cggtgatgga ctaccccgcg
     2281 ccgcgggcaa agctcggcgc cgatggagtg ccggatcttt cggatgcgta tgcaaaagga
     2341 atcggcgaat gggacaaagt gtcgatcgct tacggctatc aggactttcc gccgggaaca
     2401 aacgaagccg cagcactcga tcgcacgttg agcgacgcct tcaaacgtgg cctgatgtac
     2461 ttaaccgatc aggatgcgcg accgccgagc gccgcgagtt cggtggctca cctttgggac
     2521 aacggtgcaa atgtgattga cgagctcggc aacgtgatga aagtgcgtgc tgcagcatta
     2581 cgacgctttg gcgagaacaa cattcgcgaa ggcgcgccga tggccacgat tgaagacgtc
     2641 ctcgttccga tctacatgta tcaccgttat caggtcgaag cggctgcgaa agtgatcggc
     2701 ggacaagact acacgttcag cataaaaggc aaaggtgatc gtaatccgca aatcgttccg
     2761 gctgaagaac agcgtcgtgc acttgcggcc gtgctcgaca cgatcaaacc cgaagcgctt
     2821 gcagtgcccg agtcgttgtt gcgtttgatt ccgccgcgtc ccgcgggttt tcagcgcacg
     2881 cgtgaagact tccgcattcg cacgcagccc gtgttcgatg cgctggcgcc tgcagaagcg
     2941 attgccgatc acgtcagtgg ctttttattg aactcggagc gagccgcgcg acttattcag
     3001 tttcacgcac gcgatcagcg caatccgagt ctcgccgaag tgatcgacaa aatatttgcc
     3061 gcgacctgga aagcgcctgc agcgactggt tacgcaggcg agatacaaca cagcgtcaac
     3121 ttcataatac tgtcagatct gatggccctc gcgtccgctg aacgcgcgtc gaatcaggca
     3181 cgcgcgatcg cgtcgtggaa aatcgaacaa ttgaaggcgt ggctcacggc gcaaagtcgt
     3241 tttccggtgg acgagaatca gcgcgcgttc atgttttatt cactcgaaca aatcaaacgt
     3301 tttcaggaag atccaaagaa gatgaacctc acgccagccc agcaacctcc cgatggccag
     3361 ccaattggaa tggattggtg gtcgcgcgcc gacgaggatt gtagttatcg aaactgatcc
     3421 ggatcctaat ccgtagtaat cggagtttgt ttccgtgtta tccgtggttt agcatacgtg
     3481 tcttatgccg gaagctgaag agtacgttgg cgaggtgctt gacgataagt accgcctgga
     3541 acacctgctt ggcaaaggtg ggatgggcgc agtctacctc gcaacacacc tcggcacaga
     3601 acgttacgtc gcgctgaaac tgatctcgcc gcagttcatg cgcaataaag agtttgtcga
     3661 gcgcttcaaa cgcgaggcgc gcgccgcagg tcgtttgcgc catccgaacg tcgtcgacgt
     3721 aactgacttt ggtttcgcgc gagtcgaagc cgggcaggtc gcgtacctcg tcatggaata
     3781 tctcgacggc tgcacgctcg gcgacgttct ggctgaggaa aaacggctgc cgctcgagtg
     3841 ggttgccgac attctcgatc aggtttgttc ggcggttcac gaggcgcatc aacagggaat
     3901 cgttcatcgc gatctaaagc ctgacaacat ctggctcgaa ccaaatcgtc tcggcagcta
     3961 tcgcgttaag gtgctcgact tcggtatcgc caaagtcggc gaagtcgcgc ctcccgcact
     4021 cgatgaagat cccgcagcga caatcatcat tgacgatcac agtgcaccga atggcgcaca
     4081 actcgagtca gacacactga tgcggcccgt aggcgatgaa tcgctcacac agcaattcag
     4141 gatcaaaggt gacggcgagg gcgtcggcac tctgcttcaa gatccgctga caaatccgct
     4201 attgagcaaa tcagatcacg tagccgacga cgatggcacg ttgctcttca ccgatcccgg
     4261 aacacatagt gccgcgcgtc gcaccgctca tatcgcaact tcgccgcagg aaggcgccgc
     4321 gcttacgcgt gtgggcgcga tcatgggcac gccgctttac atgtcgcccg agcagtgtgg
     4381 cggtggatac gtcgatacac gttctgacat ttatagtctc ggcgtcatcg cctaccagat
     4441 gcttgccggt gaaccgccat ttgctggaaa tacatcggcg gtcatgcgtg ctcatcgcga
     4501 gcagatacca acacacctgc gcgagcgtga ggccaagatt ccgaaacgcg ccgccggtgt
     4561 agtgatgtca gccctttcga aaaatccagc cgagcgtccg caaacggcgt ttgcttttgg
     4621 tagtgcgatg cgcgcgcaga gcgaaggcgt cggaacgctc tatcggcgtg cgttctcact
     4681 ctacagcgag tacttcccga aattccttaa gctcagcctg atcgcgcacg tgccgatgat
     4741 cgttaccagc attctgctgg tcagcgcgtt cgtctggtac aagtattcgc cgccggtcgg
     4801 gatcctgcac aaagtgattg tgtactgcgc gatcgcaatc ctgagtctaa tgaagtttgt
     4861 ggcgacgacg attgcggtcg gtgtcatttc gggtgtgact gcgatcatca ttacgcaact
     4921 aacagtggcg cctttgcgac cagtgaagtt gcgtgaggct ttcgcagtga tgcgcaaacg
     4981 atggcgtccg tttgttaaga cgctcgtcag ggtgacgctg cggatcttta tcggtcttat
     5041 cctgcttgtg gttcccggca tgatcatgtg gatgcgctac tcgctatatg ccccagttgt
     5101 tctcatggaa ggactggaag gtaaagcgg
//
LOCUS       DASSFQ010000285         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_738197, whole genome shotgun sequence.
ACCESSION   DASSFQ010000285 DASSFQ010000000
VERSION     DASSFQ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738197"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>2396
                     /locus_tag="VFH96_10365"
     CDS             122..>2396
                     /locus_tag="VFH96_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974418.1"
                     /translation="MPKDSTDLSFLSGGGEMGERMRAFDWSSSPVGPAAQWPQSLKTV
                     VSICIGSRYPIVLWWGNPEYTMFYNDGYIPILGVTKHPGWLGRSGREAWKEIWSTIGP
                     MLDSVFRTGEATWSEDLLLVMDRNLPREETYFTFSYSPIRGDKDKIDGIFCACYETTG
                     RVVGERRLQTLRDLGRIEVKSAEEACQIALNTLAANPYDVPFALVYLLDEDAREAQLI
                     CASGIEDDSTAAPALITMNDGATWPLRDVFETGAAKVVSDVEERFGSLPGGPWPESCN
                     TALILPIGASGYAKPSGFLVAGFSPRRLVDAEYQSFFELVVGHVGTAISNARAYEEER
                     KRAEALAEIDRAKTTFFSNVSHEFRTPLTLMLGPLEETLADTSLSASVHDHIEVAHRN
                     SIRLLKLVNTLLDFSRIEAGRIEAVYEAVDLQALTTELASMFRSAIERAGLKLIVDGL
                     PFEAEVYVDREMWEKIVFNLLSNAFKFTFAGEITVALKKSGENVELEVTDTGTGIPEE
                     ELPNLFERFHRVKGARGRSYEGSGIGLALVQELVRLHRGAVRVKSEFGRGSTFTVSIP
                     TGSAHLPAERLGGARTLASTTVRSDAYVDELLKWIPDETNHLETFALPETSAAPVDQP
                     RARILLADDNTDMREYLRRLLATKYDVQAVAEGETALAAALSQKFDLVLSDVMMPKLD
                     GFGLLKALRADEKTRTTPVILLSARAGEESRVEGMGAGADDYLIKPFSARELMARVEA
                     HLNLQRVRRESEEAVLQL"
BASE COUNT          508 a          655 c          713 g          520 t
ORIGIN      
        1 atgtccgaca tgctttagct tgtcgggatt gcgcgacaaa ctgaagtttg tcggacattg
       61 acagaaaatc agtcgccgag tttaattagc gcaaccccca tccctaatcc cttaacgatt
      121 aatgcccaaa gactctaccg atctgagttt tttaagtggc ggcggcgaaa tgggcgagcg
      181 catgcgtgcg ttcgactggt ccagcagccc ggttggacca gcagcccagt ggcctcagtc
      241 tctcaaaacc gtggtttcca tctgcatcgg ctcgcgttat cccatcgtgc tctggtgggg
      301 caatcccgag tacacgatgt tctacaatga tggctacatt cccattctcg gcgtcaccaa
      361 acatcccggc tggctcggtc gctccggtcg tgaggcgtgg aaagagatct ggtccactat
      421 cggcccgatg ctcgacagcg ttttccggac aggcgaggca acgtggtctg aagatctgct
      481 cctcgtgatg gatcgcaacc tgccgcgcga agaaacttat ttcacttttt cttacagtcc
      541 gattcgcggc gacaaagata agatcgacgg catcttttgc gcttgctacg aaaccaccgg
      601 acgcgtggtc ggcgagcgac gtttgcaaac acttcgcgac ctcggtcgta tcgaagtgaa
      661 atcagccgaa gaagcttgcc agatcgcact caacacgctc gccgccaatc cttacgacgt
      721 gccgttcgcg ctcgtctact tgttggatga agacgcacgc gaggcgcagt taatttgcgc
      781 gagtggaatc gaagacgatt ctactgccgc tccggcactc atcacgatga acgacggtgc
      841 gacgtggccg ctgcgcgacg tgtttgaaac cggcgcggcg aaggttgttt ctgacgtcga
      901 ggaacgattc ggctcgttgc ccggtgggcc atggccggag tcgtgcaaca cggctttgat
      961 tttgccaatc ggcgcgtctg gttacgcgaa gccgtcggga tttttagtgg cgggtttcag
     1021 tccgaggcgt ctcgtcgacg cagagtatca gagtttcttc gagctggtgg ttggtcatgt
     1081 cggcaccgcg atctcaaacg cgcgcgcgta cgaggaagag cgcaaacgtg ccgaagcact
     1141 cgccgagatc gatcgtgcca aaacgacctt cttttcaaac gttagccacg agttccgcac
     1201 gccgctgacg ctcatgcttg gtccactcga agagacgctc gcggatacaa gcttgtctgc
     1261 gagcgtacac gatcacatcg aggtggccca tcgcaacagt atccgcctgc tcaaactcgt
     1321 gaacaccctc ctcgatttct cacgcatcga ggcggggcgt atcgaagctg tttacgaagc
     1381 cgtcgatctg caagcgctga ctaccgagct ggcgagcatg tttcgctcag caatcgagag
     1441 agccggactg aagctgatcg ttgatggttt gccgttcgaa gccgaggtct acgtcgatcg
     1501 cgagatgtgg gagaagatcg tttttaacct gctttcgaat gcattcaagt ttacgtttgc
     1561 cggcgagatc acggtggcgc tgaagaaatc gggcgagaac gttgagctgg aagtgaccga
     1621 caccggaacc ggaattccgg aggaggagct gccaaacctg ttcgaacgtt ttcatcgcgt
     1681 taaaggcgcg cgtgggcgga gttatgaagg atccggaatc ggactcgcac tggtccagga
     1741 actcgtaaga ctccatcgcg gcgccgtgag agtcaaaagc gaattcgggc gcggaagtac
     1801 atttaccgtc agtattccta cgggctccgc gcatttgcct gccgaacgac tcggcggcgc
     1861 gcgtacgctc gcatccacga cggtgcggag cgacgcgtat gttgatgagc tgctgaagtg
     1921 gattcctgac gagaccaatc acctcgagac gtttgctcta ccggaaactt ccgcagcgcc
     1981 ggtagaccaa ccgcgcgctc gcatcctgct cgcagatgac aacaccgaca tgcgtgagta
     2041 tctacggcga ctactggcga cgaaatacga cgtccaagcc gtggccgaag gtgaaactgc
     2101 gctcgccgcc gcgcttagtc agaaattcga tcttgttttg tcagacgtga tgatgccgaa
     2161 gcttgatggc tttggtttgc tgaaggcgct gcgggctgac gagaaaacac gaactacgcc
     2221 cgtgatactg ctgtcagcgc gggccggtga ggagtcgcgt gttgaaggta tgggcgccgg
     2281 agcggatgac tatttgatca agccgttttc agcgcgcgag ctgatggcgc gggtggaggc
     2341 acacttgaat cttcagcgcg tacgtcgcga gtcggaagag gcggtgttgc agttga
//
LOCUS       DASSFQ010000286         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_881081, whole genome shotgun sequence.
ACCESSION   DASSFQ010000286 DASSFQ010000000
VERSION     DASSFQ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881081"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFH96_10370"
     CDS             complement(<1..1150)
                     /locus_tag="VFH96_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974419.1"
                     /translation="MNLRTVSLVVGLLLLALVSSCRPTNNLPTKSSPQYNEIVRTFYI
                     GLAALQVGHDVQADAKLQQFTQLAPGEPAGWANWGLLALRQRNYDTAAERLNKSRDLA
                     PDNSDIFYLLGLLESSRGNTAEAINDLRKSVELDQKNLIATYKLAEEVERQGDEKSAQ
                     EFQSLIQKILAVQPDNLAGLVESTRIAAKRGDAETVKSSVAKIVARSSAWPEEVRAQV
                     NAVDTAAKSGDLNSAATRTSFLRNVLMRLPEYRRDLAVIKPPPGEEATPFTHFLKMEA
                     PVFTAAPPDTTITFKPEWVTAAGAEPADWIGAISLSGSGAPVIATATGREVSLTSGAK
                     FPFPGGPSNKPPDTRSILPVDFNYDFKTDLVLAGVGGIRFFRQDSPQSF"
     gene            1259..>2551
                     /locus_tag="VFH96_10375"
     CDS             1259..>2551
                     /locus_tag="VFH96_10375"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET6974420.1"
                     /translation="MRKPLLLALALTACFLTTTAHLQNEPAKREDAYRANNLGVALLE
                     QFKHKEAADAFRRALQLDPGLVLARINLGIALFNIPDLPAAQKELLAAIAAAPSAPQP
                     HYLLGLAAKLQNKPEEAIASFQRVLRVDPNDVGANVNIGQLYSQQRKYTEAIAALRLA
                     VAAEPYNATALYNLGTSLIRAGQREEGQKVIAQFQELRQRGTGTTLGSNYLEQGRYAE
                     AVASTGAEPELVDKRTPEVVFADANVLPEEEAGAATLFDYDLDGDLDLVAVAGTRQRL
                     LRNDGGKFTDVTAQSGALAKAATAAGAAIVAGDYDNDTKPDVFVLRAGTSTLYHNDGN
                     GSFSDVTANAKIPSALTGGTTAAFVDYDHDGDLDLLLGGDGLLLLRNNGDGTFADQTA
                     AAKLTDKVHARAIVPTDFDNRRDIDLLVASTEKVSLWRN"
BASE COUNT          542 a          722 c          728 g          559 t
ORIGIN      
        1 tgaacgactg cgggctgtcc tgccggaaaa aacgaattcc accgacaccg gccaaaacga
       61 gatctgtttt gaagtcgtag ttaaaatcga cgggcaagat gctgcgagtg tcgggcggtt
      121 tattggatgg tccaccgggg aacgggaact tcgcgccgct ggttagactc acttcgcgcc
      181 cggttgcggt tgcgatcacg ggagcgcccg atccgcttaa agagatcgcg ccgatccaat
      241 cggctggctc tgcacctgcg gcagttaccc attccggttt gaatgtgatg gttgtgtccg
      301 gaggcgcggc ggtgaagact ggcgcttcca ttttcagaaa gtgtgtgaaa ggcgtcgcct
      361 cttcgccagg cggcggtttg atcactgcga gatcgcgacg atactcgggt aaacgcatca
      421 acacgttgcg caggaaactg gtgcgcgttg cggctgagtt gaggtcgccg cttttcgccg
      481 ccgtatcgac agcgttgact tgtgcacgca cttcttcagg ccatgcagac gaacgagcca
      541 cgatcttcgc tactgatgat ttaaccgttt ccgcgtcgcc gcgtttggct gcgatacgcg
      601 tcgattcgac gagaccggcg agattgtcag gttggacggc aaggatcttt tgaatcagcg
      661 attgaaattc ctgtgcgctc ttctcgtcac cctgtcgttc aacttcctcg gcaagtttgt
      721 acgtggcgat aaggtttttc tggtccaact cgaccgactt gcgcagatcg tttattgctt
      781 ccgcggtgtt gccgcgactg ctttccaaga gtccgagcag gtaaaagatg tcgctgttgt
      841 caggcgcaag atcgcgtgat ttgttgagtc gctcggcggc ggtgtcgtag tttcgttggc
      901 gcagtgcgag tagtccccag ttggcccaac cggccggttc gcccggagcg agctgcgtaa
      961 actgctggag ctttgcgtct gcctggacgt cgtgaccgac ctgcaacgca gcgagtccga
     1021 tgtagaacgt gcggacgatc tcgttgtatt gcggagatga tttggtcggg agattgtttg
     1081 tgggacggca cgagctaacc agggcaagca acagcaagcc cactacgagg gaaactgttc
     1141 ttagattcat acgcttcatc cacagattac accgattttt acaaacatct gcgtaatctg
     1201 tggataactt tctttgtacc tttgcgcctt tgcgtgaaac tcagcacaat gtaacacaat
     1261 gcgtaaaccc cttcttctag ccctggcgct aacggcttgt tttctgacaa ctacggctca
     1321 tcttcaaaac gaacctgcta aacgcgagga cgcgtaccgc gcgaataacc tcggcgtcgc
     1381 gctgctcgag cagttcaaac acaaagaagc ggcggatgct ttccggcgcg cgctgcaact
     1441 cgatcccggt ctcgtgctcg cgcgcatcaa tctcggtatc gcgctcttca atattcccga
     1501 tctgccggcc gcgcaaaaag agttactagc tgccatcgcc gcggcgcctt cagcaccaca
     1561 gccgcactac ctgctcggtc ttgccgcgaa gcttcagaac aaacccgaag aagcgatcgc
     1621 ctcgtttcaa agagtgttgc gcgtagatcc aaacgacgtg ggcgcgaatg taaacatagg
     1681 ccagctctac tcgcagcaac gaaagtacac ggaagcgatc gctgcgctgc gtctggctgt
     1741 agctgctgag ccatacaacg ccaccgcgct ctataatctc ggcacctcgc tgatacgtgc
     1801 gggtcagcga gaagaaggtc agaaggtcat cgcgcaattt caggaactgc gacagcgcgg
     1861 caccggcacg acgctcggat caaactatct cgaacagggc cgctacgctg aagcggttgc
     1921 ctcaactggc gctgaaccgg agctggttga taagcggact ccggaagtcg tgtttgctga
     1981 tgcgaatgtg ctgcctgaag aagaagcggg cgcggcaacg ctctttgatt acgatctcga
     2041 tggcgatctc gatctcgttg cagtcgcggg cacgcgccaa cgcctcttgc gcaatgatgg
     2101 tggcaagttt accgatgtga ctgcgcaatc aggcgcactt gcaaaagcag cgacagcagc
     2161 gggcgcggca atcgtcgccg gcgattacga caacgacacg aagcctgatg tcttcgttct
     2221 tcgcgcgggc acgagcacgc tctatcacaa cgacggcaac ggcagttttt cagacgtaac
     2281 tgcgaacgcc aaaatcccat ctgcgctaac tggtggaacc actgcggcgt tcgtggatta
     2341 cgatcacgat ggcgatctcg atctattgct cggcggcgat ggcttgttgt tgttgcgaaa
     2401 caacggcgac ggcacgtttg ccgatcaaac cgcagccgca aagctgacgg acaaagtcca
     2461 cgcacgcgcg atcgttccca ccgacttcga caaccgccgc gatatcgatc tgctcgtcgc
     2521 ctcgactgag aaagtctcgc tctggcgcaa c
//
LOCUS       DASSFQ010000287        12978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2228335, whole genome shotgun sequence.
ACCESSION   DASSFQ010000287 DASSFQ010000000
VERSION     DASSFQ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228335"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..545)
                     /locus_tag="VFH96_10380"
     CDS             complement(45..545)
                     /locus_tag="VFH96_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6974421.1"
                     /translation="MFCPKCATQNLDGASFCRSCGANISLVPQALSGQLVQPQPPVTE
                     VDGACRTRRGRELTLEEPFKKFFTGIAFVIIAIVLSRGIGYGWWFWMLIPAFVLMGTG
                     IAQFIRLQEHARKAALAPPPLGRAFVEPPRTPEGLRAPVSSVTEGTTRHLGVEAPTRH
                     LDHEQR"
     gene            complement(546..1325)
                     /gene="rlmB"
                     /locus_tag="VFH96_10385"
     CDS             complement(546..1325)
                     /gene="rlmB"
                     /locus_tag="VFH96_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET6974422.1"
                     /translation="MTNRSTHDLIYGPNAVLEALRAGKRQIETITILESARPDRLKSL
                     LDLAREKGVPVHRVPRLDIDRSLGDVRHQGVVARVAAARYADADDLLDQLETKIGTDD
                     PPLVLGLDGVEDPRNMGSVLRTAECSGAHGVFIAERRAVGLTSVVAKVAAGALEYVPV
                     ARVTNLVRLIEQLKERNIWVVGAAAEATQLYTDWDWRLPSAIFLGSEGHGLHRLVREH
                     CDTLVRIPVSGKLESLNVSVAAGVLLYEARRQRTLNVKGEK"
     gene            complement(1363..2397)
                     /locus_tag="VFH96_10390"
     CDS             complement(1363..2397)
                     /locus_tag="VFH96_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974423.1"
                     /translation="MPKDNENIPFLGPVEPSEPLGFPAEQMIRCDECLRANPPTRVSC
                     LYCVAPLPLTESSARLRKPVLRQPEKHQLGYNNILLPAEGVATEVINEAAALVKLSAE
                     NVQQLLSQSVPLPVARTASREEAELVTLRLQDLGLQCVTVGDEELGSSSVVKRVRAMS
                     FADERVVIYHSGTEEASILWSDVILILTARLIETTVEIKERMTRKKENEIIDTNEFFR
                     DELVIDFYTKTPSFTWRVGTTGFDFSCLGSEKALVANENINRLQKVMVAKAVNAEFDD
                     SYQRVRNLLELAWSTQPETQSSGWRRERPGLRSIGVATTKSNEVQFTRYSRLRYYIAN
                     KSFTGLTEFS"
     gene            2429..3295
                     /locus_tag="VFH96_10395"
     CDS             2429..3295
                     /locus_tag="VFH96_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974424.1"
                     /translation="MRKSEAMRMFAGKLLILTLAVLTTGACAAFQPTDANGPAANRPQ
                     YPIALSDVGARLEEASVAWYQLSQRYGLPGKTEANLQPYTATLASLPANLPAPVYLPK
                     VGSQTAPTEEDTRESLRRFIVEWQTLIGADPNQLSLVERTDEPSGVKVARYEQRPFRY
                     PLRGGFGTLVIRFRSNGQLVGFSSNCIPNADRLQASLNALTPTVTAEQAVNHVRAQGI
                     SVLNLVNPQQVVPLPANAKIEARQLVVYAQPAKEPPSGLELRLAWEIEVTNAPISKVY
                     LDALSDEIIATT"
     gene            3389..4114
                     /locus_tag="VFH96_10400"
     CDS             3389..4114
                     /locus_tag="VFH96_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET6974425.1"
                     /translation="MHNFDPTNNISWQLGHRGRVAVFIDGNNLFHAARFHNIDIDYNK
                     LLRVLLGDGRLLRAFFYTGVDVGAERQQGFLLWMRRNGFRVIQKELKTFYDGTRKANL
                     DVEIAVDMLSLAGRYDTAVLVSGDEDFVYAVNAVAYKGCRVEIAGFRSNTAPKLIDVS
                     DYFIDLGEIADKVRKEISHGARYDEHCPMPAPYLQDQMEMQKSQTADSFEQAIRAVIE
                     AEIEEEAAEFEAATEFIRITDDH"
     gene            4124..4501
                     /locus_tag="VFH96_10405"
     CDS             4124..4501
                     /locus_tag="VFH96_10405"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET6974426.1"
                     /translation="MKIFTVQEANALLPDVRKIVGKIQRAHRKLSRYREDAKKAAEAA
                     ERGGGGFSNGVAYASDLLKLTAQLSDLEELGVQLKDFERGLVDFPSLRDGRVVLLCWQ
                     LGEGDELEWWHDVDAGFAGRTPL"
     gene            4604..5629
                     /locus_tag="VFH96_10410"
     CDS             4604..5629
                     /locus_tag="VFH96_10410"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET6974427.1"
                     /translation="MASSSPQDSTRPTEKRSTLIEFPGVSRNSMPEWRKELSERVREV
                     QEKRAREAAREAAEAERQRVEAAINPPQLELLPPAPMPAMNPLVAAALKRIERAHQTA
                     PSESRQPRTTLATAVAYAPEREDNDMSDTVPSTPELAFEPEVPEVEPVVTVEKTHHLA
                     VVTVSEPVAPPIEPPRAAPKRLIRDDNDPALNYLDSISKTLCVDDLNHTSASAVRRTV
                     CALLDLIFCALLSSPIAGAMYLTGSNLRDPQVIGVLAGSVVVVTFLYLTSLTALTGRT
                     WAMRLLSLRIIDTKTGLIPTGRQSIGRSFFYLLSLATVVGIFFALVSREGYTVHDRFT
                     RTAVVTS"
     gene            5656..7065
                     /locus_tag="VFH96_10415"
     CDS             5656..7065
                     /locus_tag="VFH96_10415"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HET6974428.1"
                     /translation="MNQTVLQRVVEELQSALIGRYCGKIYQLGPVSFAIDFGLRGEFL
                     FISVDPASPRLYLIHRRTKDLEKQSVALGAFGQMMRAKLSGAHLVGISKDPSDRIVRL
                     TFRSEELIFNRLIIQLTGRTADLFLLDELNRIVAVLREQAQTRIQQFYLAPPRPLKEP
                     ADALRLEAGPPSAQLDKHFSALDKAKAFDTKAKAMRSRVTKSIRQQRTLRENLQKDLV
                     RHGDPEEHKRTGDLLLANIATAQRDGNKVRIVDYYADGAPSIEIEVDEHRSLQEEAAA
                     RFRQYTKAKHAAGEIAERLKQIDRETENLERRLQQLDAIIQSRDKAALETFEKPAPAP
                     KATPKKSARPEKISGVRRYLSTDGYEILVGRAARDNDNLTFRVAQPNDLWMHAGDYPG
                     SHVVVRNPTRKEIPQRTIIEAAQLAGKFSQASDDAKVVIHYTERKFVSKPKGAAPGLV
                     RLSRFRSITVEPKEAVPRL"
     gene            7081..8361
                     /gene="hemL"
                     /locus_tag="VFH96_10420"
     CDS             7081..8361
                     /gene="hemL"
                     /locus_tag="VFH96_10420"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET6974429.1"
                     /translation="MTQSEKLFATAQNLIPGGVNSPVRAFRGVGGTPRFIRSARGATI
                     TDVDGNTYIDYVGSWGPMILGHADEELVAAVQEVAAGGTSFGAPTELEVELAQEVIDA
                     VPSIEMVRMVSSGTEATMSAIRLARGVTGRTKLVKFEGCYHGHADSLLVKAGSGVATL
                     GLPDSPGVPTALAENTLTLPFNDVPALEKVFAEHADIAAVIIEPVVGNMGCVPPRDGY
                     LEAVRKITRDHGALLIFDEVMTGFRLARGGAQEHYDITPDITTLGKIIGGGLPVGAYG
                     GSREIMNNIAPAGPIYQAGTLSGNPLSMTAGLVTLRRLRDKSVYDRLEAASKRLCDGL
                     ADAAGEMGVETVTNRVGSMWTSFFTSESVVDWDSANKSDRQRYGKFFHAMLDKGVYLA
                     PSQFEAGFVSLAHTDAIIDKTIEAAKKAIHRLHR"
     gene            complement(8391..10178)
                     /locus_tag="VFH96_10425"
     CDS             complement(8391..10178)
                     /locus_tag="VFH96_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974430.1"
                     /translation="MKPAPNKHPEVVYRRLPVPGSSENNAEAEPENRGLALALTPLII
                     GFVLLLGLISAVAFISVRLMNDVGFRAREVGLQRTGRLNLLWDVRLKVTRLDSEARAR
                     ARTESEARELAPPFDFRLNTNRAEVIEVARQLDTPPLSREASWQKLHNDLQAYIEITK
                     DPKVYSLKGFEKFRVVDADLNDLFLQVQSENDEVIKGVIDLQVSFKRSIITWSVVALL
                     VGALVAFGTIWEVQRHFRQMRRSMFETRRERTFTTQLLQGMVSAVAAIDNEDRIRSAN
                     PAFFRIFPNASIGASVLEKLGPDDAMKMLETVTSIRVRKATYHGRLIARVEEEDKAFD
                     VYSAPLSINGEPGQAVTLVDATEAAESERTLRRSESLAAVGQATTQVAHEIRNPLGSI
                     RLGVSMLRDSVSDPEALNTIELVERGIKHLNKLVVDVTQFSRQKELERAAVDLHESLE
                     RSIDLVSERIVEKNTTVEKQFSARSIVGQWDPYQLRQVFVNVIANAVDASQENSEVLI
                     STELLAPETNGDGQSPRHYARITIADSGRGMDKATRDRIFEPFFSTKKRGTGLGLAIV
                     KQIVELHGGRISVNSEIGKGSQFRIDLPV"
     gene            complement(10188..12923)
                     /gene="polA"
                     /locus_tag="VFH96_10430"
     CDS             complement(10188..12923)
                     /gene="polA"
                     /locus_tag="VFH96_10430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET6974431.1"
                     /translation="MPARESVAKNERPQRVFLIDSMSHIFRAFFAPMQNRAAPLATSQ
                     GFVTQAVYIFTNMLLKLLREEKPDYIAAIFESREKTFRHETFADYKANRVAMPDDLAK
                     QMPYIFRLCEVLNIPVINFPGYEADDVIGTLSQQVAEMKMQAVIVSNDKDMCQLVHDP
                     YIICMRQNSQVVKRKEPVPPIEWCDEAWVEHKFGVPADKLVDLLGLMGDSIDNIPGAP
                     GIGSKGAVQLIQEFGSIEAALAGWESVKHKKYRESLRDNADLIRQSRELARIRCDLEV
                     PWKLEELKTRPADRVAAYELFHELEFVLLMKEYADAAVQRPASAESARNYKIIQTKSD
                     LDDLVRTLFDAEHVGVAIADSTPAGTGEQECFTELQATRGIAFSTEPGFAAYVDLEKF
                     SGGMDTAVNALRDVLSNGLIEKSIHDVKRAVGLLDRFNITLEGVKDDTYLAAYLLDPS
                     RSKYELSDLAREALNVEHSSTPAVNWPDTAWQTVVAADLTAQTAHVLHNRILEKGLET
                     IYSEVELPLAPLLYRMERAGLKVDPKVLADLSNYIGQELHKLTVQIYQLAGREFNIGS
                     PKQVGEVLSDLDIQTGRKTSTGRISTSKAVLEELAQQFELPRLIIDYRELDKLKSVYT
                     DALPHQIATDGRIHCQLNQTVAATGRLSSSDPNLQNIPIRSEMGRHIRRAFVAEKGHK
                     IISADYSQLELRLLAHITQDPVMLEAFQNGEDIHARTARLVFGAKTDEELKEARRFAK
                     IVNFAIAYAIEPWGLSQRVGISRPEAKKVIEDYYNTYKGVRRYMEEIPVRAREYGYVR
                     SIYGRIRPLQGISDRNANIRKAAEREAINMPIQGTASDIVKIAMLKVDEEFKRAGLES
                     RMLMQVHDELLVEVPDKEVAQVSEILRHEMETAVSLDVPLVADVGVGDNWMDAKP"
BASE COUNT         2891 a         3742 c         3481 g         2864 t
ORIGIN      
        1 aataactgta ggggcggccc tccgtggccg cccgctgttt ggacctagcg ctgttcgtga
       61 tccaaatgac gggtgggtgc ttcaacaccg aggtgacgcg tggtgccttc agtgacactc
      121 gaaaccgggg ctctcagtcc ctccggtgtt cgcggcggct ccacaaaagc tctacccagc
      181 ggcggcggcg cgagtgcagc tttcctggcg tgttcctgca aacggatgaa ctgcgcgatc
      241 ccggtaccca ttaacacaaa cgccggtatc aacatccaaa accaccagcc atagccgatg
      301 cctctcgaca gcacaatggc gatgatgaca aacgcaattc ccgtgaagaa ttttttgaac
      361 ggctcttcga gcgtgagttc cctgccgcgc ctcgtccggc aagcgccatc gacttcggtt
      421 acgggtggct gtggctgcac gagttgaccc gaaagagcct gcggtacgag gctgatattc
      481 gctccgcaac tgcgacaaaa actcgcgccg tcgaggttct gggtagcgca cttaggacag
      541 aacatctatt tttcgccttt cacgttgagt gttcgctggc gtctcgcctc gtaaagcagc
      601 acgcctgccg caaccgagac atttaacgat tccaatttgc ccgagacagg tatacgcacg
      661 agtgtatcgc agtgttctct gaccaaccgg tgcaagccgt gtccttcgct tccgagaaaa
      721 attgcggacg gcaatcgcca atcccaatcc gtgtacaact gtgttgcttc agctgcggcc
      781 ccaaccaccc agatgttacg ttctttcaac tgctctatca atcgcacgag attcgtcact
      841 cgcgccaccg gaacatactc gagcgcaccc gcggcgacct tcgctacgac actcgtcaat
      901 ccaacggcgc gtcgctcggc gatgaaaact ccatgagcgc ccgaacactc tgcggtacgc
      961 agtactgatc ccatgttgcg tggatcttcg actccgtcga gcccgagtac taacggcgga
     1021 tcgtcggttc caatctttgt ttcgagctgg tccaacaagt catccgcgtc tgcgtaacgt
     1081 gcagccgcta cacgcgcgac aacgccctgg tgtcgcacgt caccgagtga gcgatcgata
     1141 tcgagtcgcg gcacacgatg aaccggcacg cccttctcgc gcgcgagatc gagcaacgac
     1201 ttcagacgat cgggacgagc gctttcaaga atcgtgatcg tctcgatctg gcgtttaccc
     1261 gcacgcagcg cttcaagcac tgcatttgga ccatagatta gatcgtgtgt gcttctgttt
     1321 gtcatcggag tgtttgacag gatttacaag atttcaggat tatcatgaaa attctgtcaa
     1381 tcctgtaaaa gacttgttcg cgatgtaata ccgtaaccgt gagtagcgtg tgaattgaac
     1441 ctcgttactc ttcgttgtcg ccacaccaat actgcgcagc ccgggtcgct cgcggcgcca
     1501 cccactcgat tgcgtttccg gttgcgtgct ccacgcaagc tcgagcagat ttcgaacacg
     1561 ctgatacgaa tcatcaaact ccgcattcac tgctttcgca accatgactt tctgcaaccg
     1621 gttgatattt tcattggcta cgagcgcctt ctcactaccg agacacgaga aatcaaaccc
     1681 ggttgtcccc acccgccacg tgaacgaagg cgtcttagtg taaaagtcaa tcacgagctc
     1741 gtctcggaag aattcattcg tatcaataat ctcgttctcc ttcttacgcg tcattcgctc
     1801 tttgatctcg accgttgttt cgatcaggcg cgccgttaag atcaggatca catccgacca
     1861 caagatactc gcttcttccg tgcctgagtg atagatcaca acgcgctcat cagcaaaact
     1921 catcgctctc acgcgcttta caacactgga cgaaccaagc tcttcatcgc caacagtcac
     1981 acactgcaaa ccaagatctt gcaacctcag cgtgaccagc tcagcctctt cccgactcgc
     2041 agtccgcgcc accggcagcg gtacgctttg cgatagtagc tgctgcacat tctccgccga
     2101 gagtttcacg agggccgctg cctcattaat aacttctgtg gccacgcctt cagcaggcag
     2161 tagaatgttg ttgtaaccta actggtgttt ctcaggctgc cgcaggaccg gttttcgcaa
     2221 tcgtgcagaa gattctgtca atggcaacgg agcgacacaa taaagacaac ttactcgcgt
     2281 aggtggatta gctctcaggc actcgtcgca acgaatcatt tgctctgcgg ggaaaccgag
     2341 cggctccgaa ggttcaactg gtccaaggaa gggaatgttt tcgttatctt taggcaacag
     2401 ctcctccaag tatttcaatt atacgacgat gagaaaatcc gaggcgatga gaatgtttgc
     2461 tggaaaacta ttgatactaa cactggcagt cttgactacc ggcgcgtgcg cagcattcca
     2521 gccaactgac gctaacggac cagccgccaa tcgcccgcag tatccgatcg cgttgtcaga
     2581 cgtcggcgcg agactggaag aagcttcggt cgcgtggtac cagttgtcgc aacgttacgg
     2641 tctgcccgga aagactgaag cgaacttgca gccgtacaca gcaacactcg cgagcttgcc
     2701 tgcgaacctg cccgcgccgg tgtacctacc gaaagtcggc tcacaaacag ctccgactga
     2761 agaagacaca cgcgagtcgc tgcgaaggtt catcgtcgaa tggcagacat tgatcggagc
     2821 agatccgaat caactgtcgc tcgtcgaaag aactgacgag ccatcgggtg tcaaagtagc
     2881 tcgatacgaa cagcggccgt ttcgctatcc gttgcgcggc ggcttcggca cgctcgtgat
     2941 tcgctttcgt agcaacgggc agctcgtcgg attttccagc aactgcatcc cgaacgcgga
     3001 tcgtctgcaa gcatcgctaa acgctttaac accgacagtc acggccgagc aggcggttaa
     3061 tcacgtcaga gcccagggca tctcggtcct gaacctggtc aatccacagc aggttgttcc
     3121 cttgcctgcg aacgccaaaa tcgaggcgcg gcagctggtc gtctatgccc agcctgcaaa
     3181 agaaccgccc agcggcctgg aattacggct ggcctgggaa atagaggtca caaacgcccc
     3241 cattagcaag gtctatctag acgcactttc tgatgaaata attgcaacaa cttagtgacg
     3301 gtccataaca gttttccaac ttgaccatga gacagcaaag cagcattcta gattcaaaaa
     3361 aaccaagggg tatatgcgga gtgagtttat gcacaatttc gatcccacta ataacatttc
     3421 atggcagctc ggtcatcgcg gtagagtcgc agtttttata gacggtaata atcttttcca
     3481 tgccgcccgt tttcacaaca tcgacatcga ctacaacaaa ctgctccgtg tgttgttggg
     3541 tgatggacga ctgctgcggg ccttcttcta caccggtgtg gacgtaggcg ccgagcggca
     3601 acagggcttt ctcttatgga tgcgccgcaa cggttttcgc gtcattcaaa aagagctgaa
     3661 gaccttctat gacggtaccc gaaaagcgaa ccttgacgtt gagatcgctg tagacatgtt
     3721 gtcgctggcc gggcgttacg atacggcggt gctcgtctca ggcgacgagg attttgtgta
     3781 tgcagtcaac gccgttgctt acaaagggtg tcgggtggag attgcgggtt tccgctcaaa
     3841 cacagcgccg aagctgatcg acgtttcgga ctactttatc gacctcggcg agattgcaga
     3901 caaagtgcgc aaggagattt cgcacggagc gcggtatgat gagcactgcc ccatgcccgc
     3961 gccgtatcta caggatcaga tggagatgca gaaatcccag acggctgaca gtttcgaaca
     4021 ggccattcgc gcagtgatcg aagccgagat tgaagaagaa gcggcggagt ttgaggcagc
     4081 gactgagttc atcaggatca ctgacgatca ttaaccgcga agaatgaaga tcttcacagt
     4141 tcaagaggcc aacgcgctac ttcccgacgt tcgcaagatc gttgggaaga ttcagcgcgc
     4201 acaccgaaaa ctctcgcgtt accgcgaaga tgcgaagaag gctgcggaag cagccgagcg
     4261 gggcggcggt ggattttcta atggcgtcgc ttatgcgtca gacttgctga aactgaccgc
     4321 tcaactctca gacttggaag agcttggtgt ccaactaaaa gacttcgagc gcggcctcgt
     4381 cgattttcct tccctccgcg acggtcgcgt cgtgctgctc tgctggcaac tcggtgaagg
     4441 cgacgaactc gaatggtggc atgacgtcga cgccggcttc gctggacgta ctccgcttta
     4501 gtctaatccg cgttcatccg cgtttatccg cggcttaagt tcaactacct cttaatttct
     4561 cattgaccac ttaaagttat tcttgtagta ttcggcctcg agtatggctt catcatcccc
     4621 ccaagattcg actcggccca cagaaaagag atcgacgctc atcgagtttc ccggtgtttc
     4681 acgaaactcg atgccggagt ggcgcaaaga gttgagcgag cgtgtgcgcg aagttcagga
     4741 aaagcgcgcc cgcgaagccg cgcgcgaagc tgccgaagct gagcgacagc gagttgaagc
     4801 cgcgattaac ccaccacaat tggaattgct accgcctgcg ccaatgccgg ccatgaatcc
     4861 tctggtcgca gccgcgctca agcgaatcga aagagcacac caaacagcgc catccgaaag
     4921 tcgtcaaccg agaacgaccc tggcgaccgc ggttgcgtat gcacccgaac gagaagataa
     4981 cgacatgagc gatacggtcc cctcaacccc ggaactcgcc tttgagccgg aggttcccga
     5041 agtcgaaccg gtcgtgacag tagagaagac gcaccatctc gcggttgtaa cagtgagcga
     5101 gcctgtcgca cctccaatcg agccgcccag agcagcgccg aaacgtttga tacgtgacga
     5161 taacgatccg gcgctcaact atctcgattc gatatcgaag acgctctgtg tcgacgatct
     5221 gaatcacacc agtgcgtcgg ctgttcgtcg caccgtgtgt gcgctgctcg atctgatctt
     5281 ttgcgcgttg ttgagctcac cgattgccgg tgcgatgtat ctcactggca gcaacctgcg
     5341 cgacccgcaa gtgattggcg tgctggcggg aagtgtggtc gtcgtgacgt ttctttattt
     5401 gacatcgttg actgcgctga ctggtcgcac ctgggccatg cgcctgcttt cactgcgtat
     5461 aatcgacacg aagactggtc tgattcccac cggtcgacaa tcaatcggtc gatcgttctt
     5521 ctacctgttg tcgctcgcga cggttgttgg aattttcttt gcgctcgtta gtcgcgaagg
     5581 ctacacggtc cacgatcgct tcacccgtac ggctgtagtc acttcctaac gcagataaac
     5641 gcggatttta cgcggatgaa tcagacagtc ctgcagcgcg ttgttgaaga gcttcagagc
     5701 gcgttgatag ggcgctactg cggaaaaatc tatcaactcg ggccggtttc gtttgcgatc
     5761 gactttggat tgcgaggtga gtttctcttc attagcgtcg atccggcgag cccgcgtctt
     5821 tatctgattc accggcgcac aaaagatttg gagaagcagt cggttgcgct cggtgcgttt
     5881 ggtcagatga tgcgggcgaa gctcagtggc gctcatttgg ttgggatttc caaagatccg
     5941 tcggaccgga tcgttcggct cactttccgc agtgaagaac tcatttttaa caggctcatt
     6001 attcaactta ccggccggac ggccgatctc tttcttcttg atgagctgaa tcgaatcgtc
     6061 gcagtcttgc gcgaacaggc gcagaccagg attcagcagt tctacctcgc gccacctcga
     6121 ccgctgaaag aacccgcaga cgcgctccgg cttgaggctg gaccaccatc cgctcagctc
     6181 gacaaacatt tctcagcact cgataaagcg aaagcgttcg atacgaaggc gaaagccatg
     6241 cgctcgcgcg taacgaagtc gattcgtcag cagcgcacgt tacgagagaa cctgcagaaa
     6301 gatcttgtgc gccacggcga tccggaagaa cacaagcgaa ccggcgatct tttgctcgcg
     6361 aatatcgcga ctgcgcagcg tgacggaaac aaggttcgga tcgttgatta ctatgccgac
     6421 ggcgcgccct cgatcgagat cgaagttgac gagcatcgtt cgcttcagga ggaggccgct
     6481 gcacggtttc gacaatacac aaaagcgaaa cacgcagccg gcgagatcgc tgaacgtctg
     6541 aaacagatcg atcgcgaaac ggagaatctt gagcgtcgct tacaacagct cgacgcgatc
     6601 atccagtcgc gggataaagc ggcgttggaa acgtttgaga agccggcgcc cgcaccgaag
     6661 gctacgccga agaaatctgc caggcccgag aagatcagcg gtgtgcgccg ttacctatca
     6721 acggacggtt atgaaatcct cgtcgggcgg gcggcacgcg ataacgacaa tctcaccttt
     6781 cgcgtggccc aacccaacga tctgtggatg cacgccggcg attatcccgg ctcgcacgtg
     6841 gtcgtgcgta atccgacacg caaagagatt ccgcaacgca cgatcatcga agccgcgcag
     6901 ctagctggaa agttcagcca ggcgagtgat gatgcgaagg tggtcattca ctataccgag
     6961 cgaaagtttg tatcgaagcc aaaaggcgcc gcccccggcc tcgtccgcct ctcgcgcttc
     7021 cgctccatca ccgtcgagcc caaagaagcc gttccccgtc tgtaatgata ttcttcgagc
     7081 atgactcaat cggagaaact attcgcgact gctcaaaacc tgatccccgg tggtgttaac
     7141 tcgcccgtcc gtgcgtttcg cggcgttggc ggtacaccgc gatttatccg ctcagcacgc
     7201 ggcgctacga ttactgacgt cgacggcaat acttacatcg attacgttgg gtcatgggga
     7261 ccgatgatcc tcggtcacgc ggatgaggaa ctcgtcgctg ccgtgcagga ggtcgctgcc
     7321 ggaggcacga gctttggcgc acccactgag cttgaagtcg agctcgcaca ggaagtcatc
     7381 gacgcagtcc catctatcga gatggtgcgc atggtgagca gcggcaccga agcaacgatg
     7441 tcggccattc gtctcgcgcg cggcgtgacc ggccgcacga agctggtgaa gttcgaaggt
     7501 tgttatcacg gacacgccga cagtctgctc gtcaaagccg gctcaggcgt cgccacactc
     7561 ggattgcctg acagtcccgg cgtacccact gcacttgcgg aaaacacgct cacgcttccc
     7621 ttcaacgatg ttccagcact cgaaaaagtc ttcgccgaac acgctgacat cgccgcagtg
     7681 atcattgagc ctgtggtcgg aaacatgggc tgcgtaccgc cgcgtgacgg ctatctcgaa
     7741 gcagtgcgca aaatcacgcg cgaccacggc gccctcctga tcttcgacga agtgatgact
     7801 ggctttcgcc tcgctcgcgg cggcgcgcag gaacactacg acatcacgcc tgacatcacg
     7861 acgctcggaa agatcatcgg tgggggcttg ccggtgggag cttacggcgg cagtcgcgag
     7921 atcatgaaca acatcgcgcc cgcaggtccg atctatcagg cgggcacgct atcgggaaat
     7981 ccattatcga tgactgcggg cctcgtcacc ctgcgccggc tgcgtgataa atctgtttac
     8041 gatcgactcg aagcggcgag caagagattg tgtgatggac tggcggacgc agcgggagaa
     8101 atgggcgtcg agacggtgac aaaccgagtc ggttcgatgt ggaccagttt ttttacgagt
     8161 gaatcggtgg tggattggga cagcgctaat aaatccgacc ggcagcgcta cggaaagttt
     8221 ttccacgcca tgttggataa aggcgtgtat ctcgcgccgt cacagttcga ggccggtttt
     8281 gtgagcctgg cgcatacaga cgcgataatc gacaagacta tcgaggcagc gaaaaaagcc
     8341 atccacagat tacacagata attctctgtg taatctgcgt aatctgtgga ttaaacaggt
     8401 aaatctattc ggaattgaga gccttttccg atctcgctat tgacgctgat gcggccgccg
     8461 tgcagctcga cgatctgctt cacgatcgcc aatcccaatc ccgttccacg cttcttcgtt
     8521 gagaagaacg gttcaaagat tcgatcgcgc gtggccttgt ccattcccct gccactgtcg
     8581 gcgatcgtga tgcgcgcgta atgacgcggc gactgcccgt caccattcgt ctccggcgcg
     8641 agcagctccg tcgagatcag cacctccgag ttttcctggc tcgcgtcgac agcgttcgca
     8701 atcacattca cgaacacctg tcgcaactgg taaggatccc actggcccac gatcgagcgc
     8761 gcactaaact gcttctccac cgtcgtgttc ttctcgacga ttctttcgct gaccagatcg
     8821 atgctgcgtt cgagcgattc gtgcaagtcg acggcagctc gctccagctc cttctgacgc
     8881 gagaactgcg tcacgtcaac cacaagcttg ttgagatgct tgatgccgcg ctcgacgagc
     8941 tcgatcgtat tcagcgcctc cggatccgag acgctgtcgc gcagcatcga gacgccgagt
     9001 cgaatgctgc cgagcgggtt gcgaatctcg tgtgcgactt gcgtcgtcgc ctggcccacg
     9061 gcagcgagtg attcactgcg acgcagtgtg cgctcagatt cagcggcctc cgtagcatcg
     9121 acgagcgtca ccgcctgccc gggttcacca ttgatcgaga gcggcgctga atagacgtcg
     9181 aaggctttgt cttcctcctc aacgcgcgct atcaaacgac cgtgataagt agctttccgc
     9241 acgcgaatcg aagtcaccgt ctctaacatc ttcatggcat cgtcaggtcc gagcttttcg
     9301 agcacagacg cgccgatgct ggcattggga aagatcctga agaaagcagg gttggcgctg
     9361 cgaatacgat cctcgttgtc tatcgcggcg acggcgctaa ccattccctg tagcagttga
     9421 gtcgtgaagg tacgctcgcg gcgcgtctca aacatactgc gacgcatctg gcggaaatgc
     9481 ctttgcactt cccagatcgt gccgaaagcc accagcgcgc cgactaaaag cgcgaccaca
     9541 ctccacgtaa tgatggacct tttgaaactc acttgcaggt caattacacc cttgattact
     9601 tcatcatttt cgctttgcac ctgcaaaaac agatcattca aatctgcatc gaccacacga
     9661 aacttctcaa accctttgag actatagact ttcggatctt tggtgatttc aatgtacgcc
     9721 tgaagatcgt tgtgcaactt ctgccaggac gcttctctag agagcggagg agtatccaac
     9781 tggcgggcga cctcgatcac ttcagcacga ttggtgttca agcgaaagtc aaaaggcgga
     9841 gcgagttccc gggcttccga ttcagttctc gcacgcgcac gggcttcgct atcaagcctt
     9901 gtaactttaa gccgaacgtc ccacagtaaa ttcaaccgtc ctgtgcgctg taaaccgact
     9961 tcacgagccc gaaagccgac atcattcatc aacctgacac ttataaaagc aaccgcggag
    10021 atgagtccga gcagtagaac aaagccgatg attagcggag tgagggctag cgcaagcccg
    10081 cggttctccg gctcagcttc ggcgttattc tccgatgagc caggaactgg caggcgtcga
    10141 tacacaactt caggatgttt gtttggcgcg ggcttcattt caaatcttca aggcttcgcg
    10201 tccatccaat tgtcacctac gccaacatca gccacaaggg gaacatcgag tgataccgcg
    10261 gtttccatct catgcctcag tatctcactg acctgcgcga cttctttatc cggcacttcc
    10321 accagcaact catcatgcac ctgcatcagc atgcgcgact cgaggcccgc acgcttgaac
    10381 tcctcatcaa ccttcagcat cgcgatcttc acgatatcac tggccgttcc ctgaatcggc
    10441 atgttgatgg cttcgcgttc cgccgccttg cgaatgttcg cgttgcgatc cgagattccc
    10501 tgcagcggcc tgatgcgacc ataaatcgat cgcacatagc catattcacg cgcccgaacc
    10561 ggaatttctt ccatgtaacg acgcacacct ttgtaggtgt tgtagtaatc ctcgatcact
    10621 ttctttgctt ctgggcgaga gatgcccaca cgttgcgaca agccccacgg ctcgatggca
    10681 tacgcgatgg cgaagtttac gatcttcgca aaacggcgcg cttctttgag ctcttcgtca
    10741 gtcttcgcgc cgaagacgag tcgcgccgtg cgcgcgtgga tgtcttcacc attctgaaac
    10801 gcctcgagca tcacgggatc ctgtgtgatg tgggccaaca ggcgcaattc cagctgcgag
    10861 taatcggcag agatgatctt gtggcctttc tctgcgacga aggcgcggcg aatatgtcgt
    10921 cccatctcac tgcgaatcgg aatattctgg aggttcggat cgcttgaaga tagccgtccc
    10981 gtcgcagcta cagtttgatt gagctgacag tgaattcggc catcggtcgc gatctggtgc
    11041 ggtaaggcgt cggtgtaaac actcttcagc ttgtcgagct cgcggtagtc gatgatcaaa
    11101 cgcggcaact caaactgctg cgccagctct tcaagcaccg ccttgctggt cgagatgcga
    11161 ccagttgaag tcttgcggcc ggtctgaata tcgagatctg aaagcacttc gccgacctgc
    11221 ttaggagatc cgatattgaa ctcgcgtccc gccagttgat agatctggac cgtaagcttg
    11281 tgtagctcct ggccgatgta gtttgaaaga tccgccagga ctttcggatc gactttcagc
    11341 ccggcgcgct ccatgcgata gagcaacggc gcgagcggca actcgacttc cgaatagatc
    11401 gtttcgaggc ccttttcgag aatccggttg tgcaggacgt gcgctgtttg cgcggtcaga
    11461 tcagcagcga ctacggtctg ccacgcagtg tcgggccagt tcaccgccgg tgtgctgctg
    11521 tgctcgacgt tgagcgcttc gcgggcgaga tcgctcaatt catatttgct gcgcgacgga
    11581 tcgagcaggt aagcggcgag atacgtgtcg tctttaacgc cttcgagcgt gatgttgaaa
    11641 cgatcgagca gaccgaccgc acgcttcaca tcgtgaatcg acttctcaat caaaccattc
    11701 gacagcacgt cgcgcagtgc attgactgcc gtatccatgc caccggaaaa cttttcgaga
    11761 tcgacatacg ctgcgaaacc gggttccgtc gaaaaggcga ttccgcgcgt ggcttgaagc
    11821 tcagtgaaac actcctgctc gccagttccg gcaggcgttg aatcagcaat cgcaactccc
    11881 acgtgttcgg cgtcaaatag agtgcgcaca agatcgtcga ggtcgctctt cgtctgaatg
    11941 atcttgtagt tgcgtgccga ttcggctgaa gccggtcgct gaaccgcggc gtcagcgtat
    12001 tctttcatca gcagcacaaa ttcgagctcg tggaaaagct cgtaggcggc gacacgatcc
    12061 gctggtcgcg tcttaagctc ttcaagcttc cagggcacct caagatcgca gcgaatgcgc
    12121 gcgagctctc gcgactgtcg aataagatcc gcgttgtcgc gcagcgactc gcgatatttc
    12181 ttgtgcttta ctgactccca ccccgcaagc gccgcttcaa tcgatccaaa ctcctgaatg
    12241 agttgcacgg cgcctttcga cccgatgccc ggcgcgccag gaatgttatc gatcgagtcg
    12301 cccatgagtc ccagcagatc gacgagcttg tcagcaggta cgccaaactt gtgttcgacc
    12361 cacgcttcgt cgcaccactc gatcggcggc acgggctctt tgcgtttcac cacctgcgaa
    12421 ttctgacgca tgcagatgat gtaaggatcg tggacaagct ggcacatgtc tttgtcgttc
    12481 gacacgatca cggcctgcat cttcatctcc gcgacttgtt gcgagagcgt cccaatcacg
    12541 tcatcggctt cgtaacccgg aaaattgatg accggaatgt tgagcacttc gcacagccgg
    12601 aaaatgtagg gcatctgctt ggcgagatcg tcaggcatcg cgacgcggtt tgctttgtag
    12661 tcggcaaacg tctcgtgtct gaacgtcttc tcgcgtgact cgaagatcgc ggcgatgtag
    12721 tcgggttttt cttcacgcaa cagcttgagc agcatgtttg tgaagatgta gacagcttgc
    12781 gtgacgaagc cctgcgacgt tgcgagcggc gcggcgcggt tctgcatcgg cgcaaaaaac
    12841 gcacgaaaga tgtgcgacat cgagtcgatc agaaagacac gttgaggccg ctcatttttg
    12901 gctacagact ctcgggcagg catgtgagga gattatagtt aatccgtgtc acccgtggct
    12961 aattcggcca cgtgagca
//
LOCUS       DASSFQ010000288         5299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_576198, whole genome shotgun sequence.
ACCESSION   DASSFQ010000288 DASSFQ010000000
VERSION     DASSFQ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576198"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2705
                     /locus_tag="VFH96_10435"
     CDS             <1..2705
                     /locus_tag="VFH96_10435"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974432.1"
                     /translation="ERRYVSPYHLALVTCQLDRPEEALDLLEKAYDSGDAKVLWMGVD
                     PELDPLHGHPRFNDLLRKLDHRLAALPAIAGQLRTDQESIAVLPFRVLGPPGENTGDE
                     YLGIGLTDALITRLSNVQRLVVRPTSSVLRYHGAAIEPLLAGRDLGIDYVVDGSLRRV
                     GNRIRVTAQLLSVNEGVTRWAEQFDEESTDVLQIEDSISEKVASALLPQLTGDEKRQL
                     SKRGTDSSEAFEAYLRGRYYWSSYTEVGLAKALESYKKAIKFDPDYALAYTGIADYYN
                     WLGVFGIRPFAETSAAAKQNAAKAVELDSTSAEAYSALGFATVCHDFDWAVAEGLHRR
                     SIEINPNYATGHHWYAFHLVMEGRFTESLEQMLLTRELDPLSPSVLQALGWCYYHARR
                     FDEAIATYRNMLDAVPEFPYGLVTLSWILRHTGNVEEAVQSAEKALELSGGGTFYISA
                     LGQAYAAAGRRDDARAVLDRLAQLSVHGYVSPYHLSLIHLHLDERERALELILDAYTL
                     RDAWVVWLGVEPQFDPLRGEPSFDAILRDLRHPAIYRKAAKVAGAAKRKRQADRIAPI
                     TTQILMPSPETQAGENEEARQLYTAGRYYSTRRTAEGLRQAIERFERAVEIDPQFAIA
                     HAELADCYALLNWYVEPPPNGAWERAKESAMRAVEADPSLPEGHASLGFVKLHYDRDW
                     VAAERELRKAIELKPSNQVAHRWYAYSLSAMGRHDEAYAEIERARQITPQSAVIATAV
                     ANVLFLAGKFDEAVAQSKKSLELDPGAVAAHTVLRWAYELKGMHNETMAAFEQERSFA
                     GDTPTTHAKRAHVLAAIGKREEAQEILDEIIAKRNQNWVTAYEIAIIYTWLGDFDNAF
                     RWLAQAEREHAVGFTFVRVDPHLAKLRTDPRFADLMRETEKTIP"
     gene            2723..3772
                     /locus_tag="VFH96_10440"
     CDS             2723..3772
                     /locus_tag="VFH96_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974433.1"
                     /translation="MNREQLDSWVREVVAWHFDPETGCPFWLDHAKNLSWDPRKEIQT
                     YDDLSRFGFFQDEWLRGGPVRRWVPKGLAHRPVYTFETGGSTGVPKSRINIDDFRIDY
                     EAFSDTLPDEYFPRGADWLSLGPTGPRRLRLAVEHLAQHRGGICFMVDLDPRWVIKLI
                     KMGQPKMAEQYKQHVVEQGLTLLKAHDNIRCLFTTPKLLEALCEKVSLKKMGITGVFC
                     GGTEMTPQFHRFAVEELLDGAYFAPTYGNTLMGLAVHKPRLAEDNYAIIYYPPMPRAM
                     VEVVDPDQPDRIVEYGETGRVRLTTLTKEFFMPRFLERDEAEREPPCDQYPWDGVRNV
                     RPFSRFQSSVVEGVY"
     gene            3816..5240
                     /locus_tag="VFH96_10445"
     CDS             3816..5240
                     /locus_tag="VFH96_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6974434.1"
                     /translation="MLRIPILRHGTPYVSLDIARTPHHQTREPFVEISQANAGLIRRD
                     LGRQNIGASSLQRFSTRELVAICTRAADHFTDDSLPLGDSTQTAEDYVQQVSATTGLP
                     YVLARRNMLKIKSMFANMESVLNGLTRNLDWDLLDRGFGEVDGRAMSFFPRTQSLGAV
                     LPNNSPGVHSLWIPAFPLRIPLVLKPGSAEPWTPYRIIQSLIKAGAPREAFSFYPTDH
                     GGASEILRSCGRSLLFGDSSTTGAWANDPRVEIHGPGYSKIIIGEDCVDDWEQYLDVM
                     IASIADNGGRSCVNASAVWAPAHAEEIAEALAKRLAAIVPRAADDEHAQLAPFVDAGV
                     AKRINAIIDQGLTEPGARDVTAAYRDSERLANWNNCSYLLPTIVLSDSEHPLAMKEFL
                     FPFASVLQVDQDEIPARLGPTLVVTAITKDPKLIQTLVASPRIDRLNIGALPTNQVSW
                     DQPHEGNLFEHLYARRAFQQAVAV"
BASE COUNT         1139 a         1527 c         1483 g         1150 t
ORIGIN      
        1 ccgaacgtcg ttacgtttcg ccgtatcacc tcgcgctcgt gacatgtcaa ctggatcggc
       61 ccgaagaagc actcgatcta ctcgaaaagg cttacgactc gggcgatgcg aaggtgttgt
      121 ggatgggagt cgatccggaa ctcgatccat tgcacggaca tccgcgcttc aatgatctac
      181 tgcggaagtt agaccaccgt ctcgctgccc tgcccgcgat tgccggccag ttgcgcacgg
      241 atcaggaatc gatcgcagtg ttgccgtttc gtgtgcttgg tccaccggga gaaaacaccg
      301 gcgacgaata tctcggtatc gggttgactg atgcgctgat cacacggctc agcaacgtgc
      361 agcgtctcgt ggtgcgtccg accagtagtg tgttgcgtta tcacggcgcg gcgattgaac
      421 cgctgctcgc cggacgcgac cttggtattg attatgtcgt cgatggcagt ctcagacgcg
      481 tcggcaatcg gattcgcgtg acggcgcaac tgctgagtgt gaatgaagga gtcacgcgct
      541 gggccgaaca attcgacgaa gagtcaactg acgtgcttca aatcgaggat tcgatctcgg
      601 aaaaagtcgc ctcggcgttg ttaccacaac ttactggcga cgaaaaacgc cagttgtcga
      661 aacgcggcac tgacagttcc gaagccttcg aggcttatct gcgtggacgt tattactgga
      721 gctcttacac cgaagttggg ctcgccaaag cactcgaaag ctacaagaag gcgatcaagt
      781 tcgaccctga ctacgcgctt gcttacaccg gcatcgccga ttattacaac tggctcggcg
      841 tctttggtat ccggccattt gccgaaacgt cagcggctgc aaaacaaaat gccgcgaaag
      901 ctgtcgagct ggactcaact tcagcggaag catacagcgc cctcggtttc gcgactgttt
      961 gccacgactt tgattgggcc gtagccgaag gtctgcaccg tcgctcgatc gagatcaatc
     1021 cgaattacgc gaccggtcat cattggtacg ctttccatct cgttatggag ggacgtttca
     1081 ctgaaagtct cgagcagatg ctgctcacgc gcgaactcga tccactctct ccgagcgttc
     1141 tgcaagcact gggctggtgt tactaccacg cgcggcgatt cgatgaagcg atcgctactt
     1201 accgcaacat gctcgacgcc gttcccgagt ttccgtatgg tcttgtgact ctgtcatgga
     1261 tcctcaggca caccggtaat gtagaggaag cagttcaatc tgcggaaaaa gcactcgaac
     1321 tttccggtgg tggaaccttc tacatctctg cactcggtca ggcctacgca gccgccggca
     1381 gacgcgacga tgcgagagca gtgttggacc gcttggccca actctccgtg cacggatacg
     1441 tctcgcccta tcacctgtcg ttgattcatc tacatcttga tgaacgggaa cgcgcgcttg
     1501 aactgattct cgatgcgtac actctcagag atgcatgggt agtatggctg ggagttgagc
     1561 cgcagtttga tccactgcgt ggcgagccct cttttgatgc gatcctgcgc gacttgcgac
     1621 accctgcgat ctatcgtaag gcggcaaagg tcgcaggtgc cgcaaaacga aaacgccagg
     1681 ccgatcgcat cgctccaatc accacgcaga tattgatgcc gagcccggaa acgcaggcgg
     1741 gagaaaacga agaagcgcgc cagctttaca ccgccggacg ttactactcg acgcgacgaa
     1801 ccgcggaagg cctgcgacag gcgattgaac gtttcgaacg tgcagtcgaa atcgatccgc
     1861 agtttgcaat cgcgcacgcc gagctcgccg attgctatgc gctactcaac tggtatgtcg
     1921 agccgccgcc gaacggagcc tgggaacgcg ccaaagaatc ggccatgcgc gccgtcgagg
     1981 ccgatccaag tttgccggaa ggtcatgcct cgctcggttt tgtcaaactc cactacgatc
     2041 gcgactgggt cgcggctgag cgcgagttac gcaaagcgat tgaactcaaa ccgagtaacc
     2101 aggtggccca tcgctggtac gcatacagtc tgtcggccat gggcaggcac gatgaggcgt
     2161 atgctgagat tgagcgtgcg cgccaaatca ctccacagtc ggcagtgatc gcgactgccg
     2221 tcgccaacgt acttttcctg gccggaaagt ttgacgaagc ggtggcccag tcgaagaaat
     2281 cactcgagct cgatccgggc gcggttgcgg cacacacggt tttgcgttgg gcgtatgagc
     2341 tcaaaggcat gcacaacgaa acgatggccg cgtttgagca ggagcgcagc ttcgccggcg
     2401 acacgccgac cacgcatgcg aaacgcgcgc acgtgctcgc cgcaatcggc aaacgcgaag
     2461 aagctcagga gattctcgac gagattatcg ccaagcgtaa ccagaactgg gttacggcgt
     2521 atgagattgc gattatctac acgtggctgg gcgacttcga caatgcgttt cgctggctcg
     2581 cgcaggctga acgcgaacac gccgtcgggt tcacgtttgt gcgcgtcgat ccgcacctgg
     2641 ccaaacttcg tactgaccca cgctttgctg acttaatgag ggagacagag aaaaccattc
     2701 cttaatctgt gtatctgtgg atatgaatcg agaacagtta gactcatggg ttcgtgaagt
     2761 tgtcgcctgg cattttgatc cggaaacagg ctgtcctttc tggctggatc acgccaaaaa
     2821 cctttcctgg gatccgcgca aagagattca aacctacgat gatctttcgc gttttggttt
     2881 tttccaggac gagtggttgc gtggcggtcc ggtgcgtcgc tgggtgccga agggtctcgc
     2941 gcaccggccg gtttacacat ttgaaaccgg cggcagcacg ggcgtgccga agtcgcgcat
     3001 caacatcgac gacttccgca tcgactacga agcgttcagc gacacgttgc ctgacgaata
     3061 tttcccgcgc ggcgccgact ggctttcact aggcccaact gggccgcgtc gtttgcgttt
     3121 ggcggtcgaa catctggccc agcatcgcgg cgggatctgt ttcatggtcg acctcgatcc
     3181 acgctgggtc atcaagctga tcaagatggg tcagccgaag atggccgagc agtacaaaca
     3241 gcacgtcgtc gaacagggtc tcacgttgct gaaagcgcac gacaatattc gttgtttgtt
     3301 tacgacgccg aagttgctcg aagcgctgtg cgaaaaggtg tcgctcaaga aaatgggtat
     3361 caccggtgtg ttctgtggcg gcactgaaat gacgccgcag tttcatcgtt ttgcggtgga
     3421 agagttgctc gacggtgctt acttcgcgcc cacctatggc aacacgttga tgggactggc
     3481 ggtacacaaa ccgcgcttgg cggaagacaa ctacgcgatc atttactatc cgccgatgcc
     3541 gcgtgcgatg gtggaagttg tcgatcctga tcagccagat cggatcgtcg aatacggcga
     3601 aaccgggcgc gtgcgtttga ccacgttgac aaaggaattc ttcatgccgc gctttctcga
     3661 acgtgacgaa gctgagcgcg aaccaccatg tgatcaatat ccctgggacg gcgtccgcaa
     3721 cgttcgcccc ttttcacgtt ttcaatcatc ggtcgtcgaa ggcgtttact gaatcttgtt
     3781 aatcctgaaa catcttataa atcctgtcta acaatatgct tcgcatccca atccttcgcc
     3841 acggcactcc atatgttagt ctcgacatcg ctcgcacgcc gcaccatcaa acgcgtgaac
     3901 cattcgtcga aatcagccag gccaacgcag gattgatccg ccgcgatctg ggacgacaga
     3961 acatcggcgc cagcagtctg caacgcttct caacccgtga gctcgtcgca atttgcactc
     4021 gtgccgccga tcacttcact gacgactcac tccctctcgg cgattcgacc caaaccgccg
     4081 aagactacgt gcagcaagtt tccgccacta ctggtctgcc ctacgttctc gcgcgtcgca
     4141 acatgctgaa gatcaaaagc atgtttgcaa atatggaaag tgtgctcaac ggtctcacgc
     4201 gcaatctcga ctgggatcta ctcgatcgtg gcttcggcga ggttgatggc cgcgccatga
     4261 gcttttttcc acgcacacaa tcactcggcg ccgtcttgcc aaacaattct cccggcgtgc
     4321 attcgctttg gattccagcg tttccgctgc gcattccact cgtgctgaaa ccgggcagcg
     4381 cagagccgtg gacgccgtat cgaatcattc agtcgctgat caaagccggc gcgccgcgcg
     4441 aggcgttcag tttctatccg actgatcacg gcggcgcttc cgagatcctg cgcagttgcg
     4501 gtcgttccct gctcttcggc gattcgtcga cgaccggcgc gtgggccaac gatccgcgtg
     4561 tcgagattca tggtccaggc tacagcaaga tcatcatcgg cgaagactgc gtcgacgact
     4621 gggaacaata tctcgacgtg atgatcgcgt cgattgctga taacggcggg cgctcgtgtg
     4681 ttaacgcttc ggcagtttgg gcaccggcgc acgcggaaga gattgccgaa gcgctcgcca
     4741 agcgattggc tgcaattgtt ccgcgcgcgg cagacgacga gcacgcgcag ctcgcgccgt
     4801 ttgttgatgc cggagtcgcc aaacggatca acgctatcat cgatcaaggt ctgactgagc
     4861 ccggcgcgcg tgacgtcacc gccgcatatc gcgacagcga acgcttagcg aattggaaca
     4921 actgttcata cctgctgccc acgattgttt tatccgattc agagcatccg ctggcaatga
     4981 aggagtttct gtttcctttc gcgagtgtct tgcaagttga tcaggacgag attcctgcgc
     5041 gtcttggtcc aacactcgtc gtcaccgcga tcaccaaaga tccgaagctg atccagacgc
     5101 tcgtcgcctc accgcgtatc gatcgtctta acattggcgc actgccaacc aaccaagtga
     5161 gttgggatca gccgcatgag ggcaatctct tcgaacacct ttacgcacgc cgcgctttcc
     5221 aacaggcagt tgccgtctga ggacctgagt tgagaattct ttatctcaca ggcggagcgg
     5281 gccgaatgta ttgcggcag
//
LOCUS       DASSFQ010000289         8321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1769923, whole genome shotgun sequence.
ACCESSION   DASSFQ010000289 DASSFQ010000000
VERSION     DASSFQ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769923"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /gene="ftsY"
                     /locus_tag="VFH96_10450"
     CDS             <1..890
                     /gene="ftsY"
                     /locus_tag="VFH96_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET6974435.1"
                     /translation="VFAGRKQIDEELLDQLEEVLIAADIGVQTTLEILETVRRGIARK
                     QINDLDALKLAIKTELLNILQASERQGVASELSVPEGVSPYVMMIVGVNGVGKTTTIG
                     KLAQRIKAEGNDVLICAADTFRAAASDQLAIWAERTGVPLIQQKQGTDPAAVLFDSLK
                     AAKARGSEVLIVDTAGRLHNKSNLMAELEKMKRVAGREVEGAPHETLLVVDAVTGQNG
                     LEQARQFLKVAGVTGIVLTKLDGTAKGGIAVAIAKELNLPIRYAGIGEKVDDLVVFDP
                     QQYVNSLFGNADYTDSSRT"
     gene            914..2035
                     /gene="ribD"
                     /locus_tag="VFH96_10455"
     CDS             914..2035
                     /gene="ribD"
                     /locus_tag="VFH96_10455"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET6974436.1"
                     /translation="MTGPTVWTDTDRRMMARALDLAVKGVGLVSPGPLVGCVVVSPAG
                     EIVGEGFYVFEEIKHAETIALDIAGEKARGGTAYVSLEPHAHYGRTAPCTDALIASGI
                     KRVVAPIEDLNPKVSGKGFAHLRAAGLKVETGLLSDEATQVNEAYLHYMSTARPFVHL
                     KLAVTLDGKIATRTGDSRWVTGPEARDRAHELRHAYDAILVGAGTVTTDDPLLTDRSG
                     LPRRRPLVRVVLDRQLRLSPESRLATTTSEAPVIVFGDPESPGTAALRAQGVEIEKHR
                     DLCSVLHELGTRSIQSVLVEGGSTIAGEFLDAGLVNKVTFFIAPKIVGGTEAPSAIGG
                     RGVEGMAEALELERVTVAQHGKDIEVTGYPRKGSPTDEG"
     gene            2025..2852
                     /gene="rsmA"
                     /locus_tag="VFH96_10460"
     CDS             2025..2852
                     /gene="rsmA"
                     /locus_tag="VFH96_10460"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET6974437.1"
                     /translation="MKDEGGEPYPSKRLGQHFLRDPRTIHRIIDALNPRTDETIVEIG
                     PGTGALTSVLVERAGRLIAIEFDKKLAPLLQERFGDSGNFKLVMADALTVDFCKEILP
                     AQSARLVANLPYNISTAILQRLIAQRTCIEEMVLMLQREVVERVLAPAGTSDRGFISV
                     LVEAYCEAEKLFDVAPGAFRPPPKVWSSVMRLKFRPRINAENTDDGLLWEVVSAGFAQ
                     KRKTILNNLRNASGRLQDVLKRNGGASIVLCKANVELQRRAETLTLEEWGRIVQAIR"
     gene            complement(2849..4222)
                     /locus_tag="VFH96_10465"
     CDS             complement(2849..4222)
                     /locus_tag="VFH96_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6974438.1"
                     /translation="MNNAEAIPAKKRPATRGPVPPGPKGSLIMGVMRDFNRDTLEFVT
                     RCRDYGDVVRTRFLWINAYFLYNPRDIESLLVTNAKSYRKARSLRSPFFYRLVGNGLV
                     TSEGDFWRRQRRLAQPAFHRQRISSYGEVMVQYAERAIAGWKHSEHRDIARDMNRLTL
                     EIVVKTLFNSDVSNDADHVGAILTSLVKPFASQATLKWILDNRLPTPGHRRYFNAVSE
                     IDRIVFRIIAERRASGYDEGDLLSMLLQAQDDDGSQMSDQQLRDEVMTLFLAGHETTA
                     LSLSWSWYLLAMHPEAEQKFHAELAEVLGGRAPDVSDLPKLKYTEMIAKETMRLYPPA
                     YAVGREAIEETEIGGYRVPKGTQLFAFQWVTHRDPRYFDEPDAFKPERWTNGDPSPKH
                     AYFPFGAGPRQCIGNYFAMMEVVMLLATIGQRFRFKLDPEHKVEVLPVLSLRPRHGIK
                     VITETRI"
     gene            complement(4215..4679)
                     /locus_tag="VFH96_10470"
     CDS             complement(4215..4679)
                     /locus_tag="VFH96_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6974439.1"
                     /translation="MPDLLVNLLKLPPLETTQGFIVRRAQPFELSPVRRFVAENFAPS
                     WADEISIGFARQPISVYIATIDRELAGFAAYECTRRAFFGPTGVLQSARGKGIGKELL
                     LACLWSLREMGYVYAIIGAAGPVRFYQKTVGAIIIPDSEPGIYTDLLKFKNE"
     gene            complement(4672..5253)
                     /locus_tag="VFH96_10475"
     CDS             complement(4672..5253)
                     /locus_tag="VFH96_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET6974440.1"
                     /translation="MQPKFFTSAEKFREWLERNHDCATDLLVGFHKKSSGKKSLTYAE
                     ALDEALCFGWIDGVRKNFNETSYTIRFTPRKPTSIWSNINVKHVERLQKEGRMHRAGR
                     DAYERRAPERTGIYSFENAPRELPTEYEKTFRQNKAAWKFFQEQPAGYKRLMVFRTVS
                     AKKEETRLRRLKQLIESSEKGERMGILKPKDNA"
     gene            complement(5260..6183)
                     /locus_tag="VFH96_10480"
     CDS             complement(5260..6183)
                     /locus_tag="VFH96_10480"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6974441.1"
                     /translation="MLLITVPINAQSIREQDIRAELGFLASDAMQGRGSGTVFERIAA
                     EYIGSQFQQFGLEPGGDTDSAGNKSFVQRVPVQTAASGATWNVIGVLRGTDPREAIML
                     SAHLDHLGVNETAPGDDKIFNGADDDASGSVAVMELARALSSGKRPRRTIYFVCFGSE
                     ERRGLGSRHFIDNPPVPLTQIVADLQFEMMGRPDPKVAAGTLWLTGFDRSNLGPELAK
                     QGAALVADPHPEQRFFQRSDNFPLALRGVIAHTVSSFGLHADYHRVSDEVSKIDFPFM
                     TRSVNSLVRPIQWLANSNFRPEWLPGQAPSR"
     gene            complement(6271..7320)
                     /locus_tag="VFH96_10485"
     CDS             complement(6271..7320)
                     /locus_tag="VFH96_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET6974442.1"
                     /translation="MLAANSAVQRPVDAKLLIVDSQRNLIHVPRTAFVEFLQPNDLVI
                     ANDAATLPASLSGVHQETGKTVEVRLAGRRSLNPMDVKSFSAIVFGEGDFHLRTEDRP
                     LPPALQPGDTLVLGPLRATVEAQLGHPRLVSLLFDGTPDQIWAGLASHGQPIQYSHLQ
                     TSLKLWDVWTRIAGPPVAFEPPSASFILDWHVLSDIREHRAAFATITHAAGISSTGDE
                     DLDHLLPFDEPYHIPVATAQAVELTRRRGGRVIAVGTTVVRALEAAATDDGQLRSGNG
                     VAKGKIGPATQLRVVDAILSGTHEPGTSHYELLRAFLEDSTLERATSELIAHDYKTHE
                     FGDSVLIHRLHSLHR"
     gene            complement(7307..8014)
                     /locus_tag="VFH96_10490"
     CDS             complement(7307..8014)
                     /locus_tag="VFH96_10490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6974443.1"
                     /translation="MLDPFSGLRVAITGGTSGLGLALVRELIKRNARVAFVARTRERV
                     AQVAQENPGAHGIVGDVSVKDDIYPIAMQITGELGGLDILINNASDLGPTPLALLSDT
                     ECEDLEHVFATNVFGPFRLTKALMGALAASAREGRGAVVLNVSSDAAVNPYPGWGSYG
                     SSKAALHHLSRIWNEEHAAEGVRFLSIDPGDMDTPMHAAAIPDADTATLKRPEQSAHE
                     FLEAIAAQMMVESHVSR"
     gene            8100..>8321
                     /locus_tag="VFH96_10495"
     CDS             8100..>8321
                     /locus_tag="VFH96_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HET6974444.1"
                     /translation="MSAGVQTQGEGKTRRAITKLLKTEGPIDSAQLAQRLGLTAMAVR
                     QHLYALQREGLVASEERPVPIGRPAKFWRL"
BASE COUNT         1804 a         2268 c         2463 g         1786 t
ORIGIN      
        1 cggtgtttgc cggtcgcaag cagatcgacg aagagttgct ggaccagctc gaggaagttt
       61 tgatcgcggc tgacatcggc gtgcagacga cgctggagat cctcgaaacc gtgcgtcgcg
      121 gcatcgcgcg aaagcagatc aacgatctcg acgcattgaa gctggcgatc aagacggagc
      181 tgttgaatat tctgcaagcg tctgagcgtc agggtgtggc ttccgagctg agtgttccgg
      241 aaggcgtgtc gccgtacgtg atgatgatcg tcggtgttaa cggtgtcggt aagacgacga
      301 cgatcggcaa gctggcgcaa cgaatcaagg ccgaggggaa tgacgttctg atctgcgcgg
      361 cggatacgtt tcgcgcagcg gcgtcagacc agctcgcgat ctgggccgag cgcacaggcg
      421 tgccgctgat tcaacagaag cagggaactg atccggcggc agtgcttttt gattcgctca
      481 aggcggcgaa ggcgcgtggc tcggaagttc tcatcgtcga caccgccggg cgattacaca
      541 acaaatcgaa tttgatggcc gagttggaga agatgaaacg cgtggcgggg cgcgaagtcg
      601 agggtgcacc gcacgagacg ctgctcgtcg tcgatgctgt gacgggacaa aatggactcg
      661 aacaggcgcg ccagtttttg aaagtcgcgg gcgtgacggg gatcgttttg acgaagctag
      721 atgggacggc gaaaggcggc atcgcggtgg cgatcgcgaa ggagttgaac ctgccgatcc
      781 gttatgcggg tattggtgag aaggtggatg atctcgtggt gttcgacccg cagcagtacg
      841 tgaatagttt atttgggaac gcggattata cggattcaag ccgaacttaa cgcggatttt
      901 tatgcagatg attatgactg ggccaacggt ctggaccgac actgatcgac gcatgatggc
      961 gcgtgcgctc gatctcgccg tgaagggtgt tggattagtg agtccagggc cgctggttgg
     1021 ttgtgtggtc gtcagtccag ccggtgagat tgtcggtgaa ggcttttacg tcttcgaaga
     1081 aatcaaacac gccgagacga tcgcgctcga tatcgcgggc gaaaaggcgc gcggaggtac
     1141 ggcttacgtc tcgctcgagc cgcacgcgca ttacggtcgc accgcgccct gcactgacgc
     1201 actcatcgcc tccggtatta agcgtgtcgt cgcaccgatc gaggatctga atccaaaggt
     1261 ttccggaaaa ggttttgctc acttgcgtgc ggcaggtcta aaggtcgaga caggattgct
     1321 gagcgacgaa gctacgcagg tcaacgaggc ttatttgcat tacatgtcga ccgcgcggcc
     1381 attcgtgcat ttgaaactcg cagtcaccct ggatggaaag attgcgacgc gcaccggaga
     1441 ttcacgttgg gtcaccggcc ctgaagcacg cgaccgtgcg cacgagctac gacatgctta
     1501 cgacgctatt ctcgtcggcg ccggaacagt aacgactgac gatccgctgc tgacggatcg
     1561 atcgggattg ccacggcggc gtccgcttgt tcgtgtcgtg ctcgatcgcc agttacgact
     1621 ttctcccgaa tcacgattag ccaccacgac atcggaagcg ccggtaatcg tgtttggtga
     1681 tccggaatcg ccaggcactg cggcgctgag ggctcagggc gtcgagatcg aaaaacatcg
     1741 tgatctctgc agcgtattgc acgagcttgg cacccgttcg attcagagtg ttttggttga
     1801 aggcggctcg acgattgccg gtgagtttct cgatgcgggt ctcgtcaata aagtcacgtt
     1861 cttcatcgcg cccaagatcg tcggtggtac tgaagccccg agcgcgattg gtggcagagg
     1921 tgttgagggg atggctgaag cacttgagct cgaaagagtg accgtggccc aacacggaaa
     1981 agacatcgaa gtcaccggct acccgcgcaa aggatctccg acggatgaag gatgaaggtg
     2041 gcgagcctta tccctccaag cgcctgggcc aacacttcct tcgtgacccg cgcacgattc
     2101 atcgcataat cgacgcactc aatcccagga ctgacgaaac aatcgtcgag atcggaccgg
     2161 gcacaggcgc gctcacgtca gttctcgtcg aacgcgccgg ccgtttgatt gcgattgagt
     2221 tcgacaaaaa gctagcgccg ctcttgcagg aacgattcgg tgactccgga aatttcaagc
     2281 tggtgatggc cgatgcgttg actgttgatt tctgtaaaga gatcctgcca gcacagtctg
     2341 cgcgactcgt agccaatctt ccttacaaca tctcgaccgc gattctgcaa cgattgattg
     2401 cgcaacgtac atgcatcgag gagatggttt tgatgctaca gcgcgaggtc gtcgagcgag
     2461 tactcgcgcc cgcgggaaca tcggatcgcg gattcatctc agtgcttgtt gaagcttact
     2521 gcgaagctga gaaactcttt gacgtcgctc cgggcgcctt tcgaccgccg ccgaaagtat
     2581 ggagtagcgt gatgcgattg aaatttaggc cgcggattaa cgcggaaaat acggatgacg
     2641 gcttgttatg ggaggtcgtg agtgccggtt ttgcgcagaa aaggaagact atattgaaca
     2701 atttgcgcaa cgcgtccggc cgcttgcagg atgtgttgaa gcgcaacggc ggcgcgagca
     2761 ttgtactctg caaagctaac gtcgaactac aacgccgcgc ggagactttg acccttgaag
     2821 aatggggaag gatcgtgcag gcaatccgtt aaatccgtgt ttctgtaatt actttaattc
     2881 catggcgcgg tcgaagagaa aggaccggca acacctccac cttgtgctcc ggatcgagct
     2941 tgaatctgaa gcgctgcccg atcgtggcta gcagcatcac gacttccatc atcgcgaaat
     3001 agtttccaat acattgccgc ggaccggcgc cgaaagggaa gtaagcatgt ttcggtgacg
     3061 gatcaccgtt ggtccacctt tcgggtttga acgcgtccgg ctcgtcgaag tagcgcgggt
     3121 cgcgatgcgt cacccactga aacgcaaaaa gctgcgtccc tttgggcaca cggtaaccgc
     3181 cgatctccgt ctcttcaatc gcctctctgc cgaccgcgta cgccggcgga taaagacgca
     3241 tcgtctcctt cgcgatcatc tccgtgtatt tcagtttcgg caggtcggaa acatccggcg
     3301 ctcttccgcc cagcacttca gcaagctccg catgaaactt ttgttcggcc tcgggatgca
     3361 ttgccagcag ataccagctc cacgacaacg agagcgccgt cgtctcgtgt ccggcgagaa
     3421 acaacgtcat cacttcgtca cgcagttgct gatcgctcat ctggctacca tcgtcgtcct
     3481 gcgcctgcag cagcatcgac aacaaatcgc cttcgtcgta tccactcgca cgacgctccg
     3541 cgatgattcg aaaaacaatg cggtcgattt cactgacggc gttgaaatag cggcgatgac
     3601 ccggcgtcgg cagtcgattg tcgagaatcc atttcagagt cgcctgcgat gcgaagggtt
     3661 tcaccagact cgtcaggatt gcgccgacat gatcggcgtc attggaaacg tctgaattga
     3721 agagtgtctt gacgacgatc tccagggtga gccggttcat atcacgtgcg atatcacgat
     3781 gttcactgtg cttccatccg gctatcgcac gctccgcgta ctggaccatc acctcgccgt
     3841 acgagctgat cctctgccgg tgaaaggcgg gttgcgcgag gcgccgctga cgccgccaga
     3901 aatcaccttc actcgtcacc agaccattgc ccacgagacg atagaaaaat ggcgagcgca
     3961 acgatcgagc tttgcggtag ctcttcgcgt tcgtcaccag gagagattcg atatcgcgcg
     4021 ggttgtagag aaagtaagcg ttaatccaga gaaagcgtgt gcgaacaaca tcgccgtaat
     4081 cgcggcaacg cgtcacgaat tcgagcgtgt cgcgattgaa gtcgcgcatc acgcccatga
     4141 tcaacgagcc cttaggaccg ggcggaactg gtccgcgcgt cgccgggcgt tttttagctg
     4201 gaattgcttc tgcgttattc attcttaaac ttcagcaggt ccgtataaat gccgggttcg
     4261 ctgtcgggaa taataatcgc accgaccgtc ttctgataaa agcgtaccgg ccccgccgcg
     4321 ccgatgatgg cgtagacgta tcccatttct cgcagcgacc agagacacgc aagcagcagc
     4381 tcttttccga ttcctttgcc gcgcgcggat tgcaacactc cggttggacc aaagaacgcg
     4441 cgccgcgtgc actcgtaagc cgcgaaaccg gcgagctcgc gatcaatcgt ggcgatgtaa
     4501 accgagattg gctgtcttgc aaaaccgata gagatctcgt cggcccagct cggtgcaaaa
     4561 ttctcggcga cgaacctgcg gacgggggac aattcaaaag gctgcgcgcg ccgcacgatg
     4621 aaaccttgtg tggtttcgag tggcggtaac ttcagaaggt ttacgagaag atcaggcatt
     4681 gtctttcggc ttcagtatgc ccatcctttc gcctttttca gagctctcga tcaattgttt
     4741 cagacggcgc agtcgcgttt cttctttctt tgcgctcaca gtacggaaca ccatcagcct
     4801 tttatatcct gccggttgct cctgaaaaaa cttccacgca gctttgttct ggcgaaaggt
     4861 tttctcatat tccgtaggaa gttcacgcgg agcgttttcg aatgagtaga tgccggtgcg
     4921 ttccggagcg cggcgctcgt aggcgtcgcg tcccgcacga tgcatccgtc cttctttttg
     4981 caggcgctcg acgtgcttca cgttgatatt gctccagata cttgtcggct ttcgaggcgt
     5041 gaaacgaatc gtgtagctgg tttcgttgaa gttttttcgg acgccgtcga tccagccaaa
     5101 gcagagcgcc tcgtccaaag cttctgcata cgtaaggctc ttcttacctg aagatttttt
     5161 gtggaaacca acgagcagat cagtggcgca gtcgtggttt cgctcgagcc actcgcgaaa
     5221 cttttcggct gaagtgaaga actttggttg catagctgct tagcgcgaag gagcctggcc
     5281 cggtagccac tcgggacgga agttagaatt tgcgagccac tgaatcggtc gcacgagcga
     5341 gttcaccgag cgcgtcataa acggaaagtc gattttcgac acttcatcac tcacacgatg
     5401 ataatcggcg tgcaagccga agctcgaaac cgtgtgtgca atcacgccgc gcaaagcgag
     5461 tggaaagtta tccgagcgtt ggaagaaacg ctgttccgga tgtggatcgg caacgagggc
     5521 ggctccctgt ttcgcgagct ctggaccaag gttggaacga tcgaaaccgg ttaaccacaa
     5581 cgtgccggcg gcgacttttg gatcgggtcg gcccatcatt tcaaactgca agtcagcaac
     5641 gatctgcgtg agtggaaccg gtggattgtc gatgaagtgt cgtgagccga ggccgcgacg
     5701 ctcttcgcta ccaaaacaca cgaagtaaat cgtgcggcgc gggcgtttgc ctgatgaaag
     5761 cgcgcgggca agttccatta cggcgacgct gcccgaagcg tcgtcatcag cgccgttgaa
     5821 gattttgtcg tcgccgggcg ccgtttcgtt aacgccgagg tggtccaggt gggcgctgag
     5881 catgatcgct tcgcgtggat cggtgccgcg caggacgccg atgacgttcc aggtcgcgcc
     5941 cgatgccgca gtttggaccg gcacgcgctg tacgaagctc ttgttgccgg cgctgtctgt
     6001 atcgccgccc ggctcgagac caaactgttg aaactgcgag ccgatgtatt cagcagcgat
     6061 gcgttcgaag acagtcccac tgccacgacc ctgcattgcg tcgctcgcga gaaagccgag
     6121 ctccgcgcga atgtcctgtt cgcggattga ttgcgcgttg atggggacag tgattaaaag
     6181 caagataaga agcgtgcaaa tgaaacgggg tgtgttcata ttcacgctag tatatccaca
     6241 gattacgcag agtacacaga ttcgaaaaaa tcatctgtgc aaactgtgta atctgtggat
     6301 caagacggag tcgccgaatt cgtgggtttt gtagtcgtgc gcgatcagtt cagaggtggc
     6361 gcgttcgagg gttgagtctt cgaggaatgc gcgcaggagt tcgtaatgac tcgtgccggg
     6421 ttcgtgtgtg ccggagagga ttgcgtcgac aacacgcaat tgcgttgcgg gaccgatctt
     6481 tccttttgcc acaccattac cactgcgcaa ctgaccatca tcagtcgcgg ctgcttcgag
     6541 cgcccgcacg acagtcgtcc cgacggcgat cacccgacca ccgcgacggc gggtgagctc
     6601 aacggcttga gcagtcgcga cgggaatatg gtacggctcg tcgaacggta acaggtgatc
     6661 gagatcttca tcgccggtcg atgaaatgcc cgcggcatga gtgatcgtcg caaaagctgc
     6721 gcggtgttcg cggatgtcgc tgagcacgtg ccaatcaagg atgaagctcg ctgacggcgg
     6781 ctcgaaagcg accggcggtc ctgctattcg cgtccacaca tcccataact tcaaagacgt
     6841 ctgcaggtgt gaatactgaa tcggctggcc atgactcgcc aggcccgccc agatctgatc
     6901 cggagtgccg tcgaagagca gggaaacgag gcgcggatga ccgagctgtg cttccacggt
     6961 tgcgcgcaat ggaccaagca cgagtgtatc gccgggctgc agtgccggcg gcagcggccg
     7021 atcttcagtg cgcaggtgaa agtcgccttc gccgaagacg atcgcactga aactcttgac
     7081 gtccatcggg ttcaacgatc gccgtccggc aagtcgcact tcaacagtct tgccagtttc
     7141 ctgatgcacg ccgctgagac tggcgggtag tgtcgcggca tcgttggcga tgacgagatc
     7201 gttcggttgc agaaactcga caaacgcagt gcgcggcacg tgaatcaagt tacgttgcga
     7261 atcgacgatg agtagctttg cgtccaccgg acgctgtact gctgagttag cggctaacat
     7321 gactctccac catcatctgt gctgctattg cttccaaaaa ttcatgcgca gattgttccg
     7381 gacgcttgag tgttgccgtg tctgcatccg gtatcgcagc cgcgtgcatc ggcgtatcca
     7441 tgtcacccgg atcaatcgaa agaaagcgaa caccttctgc cgcgtgctct tcgttccaaa
     7501 tgcgactcag gtgatgaagc gctgctttgc tcgaaccgta cgagccccaa ccgggataag
     7561 gattcaccgc cgcatcactc gatacgttga gcacgaccgc gccgcgacct tcacgcgcag
     7621 aagcagcgag cgcgcccatt agcgccttcg ttaagcgaaa tggaccgaag acattcgtcg
     7681 caaacacgtg ctcgagatcc tcacactccg tgtcagaaag cagcgccagc ggtgttggac
     7741 caagatcgga agcgttatta attaggatgt cgagcccgcc aagctcgcct gtgatttgca
     7801 tcgcgatcgg gtaaatgtcg tcttttacgg agacgtcgcc gacgatgccg tgcgcgcccg
     7861 gattctcctg cgcgacctgt gcgacgcgtt cccgcgtgcg tgctacaaac gccacgcgcg
     7921 catttctttt gatcagttca cgaacgaggg cgagtccgag accggaggtc ccgcccgtaa
     7981 ttgcgactcg caagcctgaa aatggatcta acatgacttt ctaaaggctc tcctttacta
     8041 agtcgaagcg tagcaccgag tattgacttt gtaaagagaa ttctttagaa atagttatca
     8101 tgagtgcggg agtgcaaacg cagggcgagg ggaagacacg tcgcgccatc accaaactgc
     8161 tgaaaaccga ggggccaatc gactccgcgc agctcgcgca acggctcggg ctcacggcga
     8221 tggccgtccg gcagcatctc tatgctttgc aacgcgaagg tctcgtggcg tcggaggaac
     8281 gaccggtgcc gatcggacgc ccggcgaagt tctggcgact c
//
LOCUS       DASSFQ010000290         5655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1915686, whole genome shotgun sequence.
ACCESSION   DASSFQ010000290 DASSFQ010000000
VERSION     DASSFQ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915686"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1176
                     /locus_tag="VFH96_10500"
     CDS             115..1176
                     /locus_tag="VFH96_10500"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET6974445.1"
                     /translation="MPITIDDLRRFTIARNFPKPTTLNRALQSMGFVQADPIRAPARA
                     QDLILRHRVNDYRAGDLERRYTSLDVEEDFFINYGYVTRALQSLMHPRPTLRQRKAKL
                     LLDFVRERGVVHPREVDEHFAHGTVTNYWGGSSNATTHLLDAMHYAGMLRIVRREKGI
                     RVYAEHKHETVPRNAAERRARMDALVDVVVRIYAPLPGSSLSYYMRRLRYAAPQWQKE
                     LPSALQRAKERLSCARVGKVDWYWPAAENISDQAIDDAVRLLAPFDPAVHDRNRFELF
                     WGWTYRFEAYTPVAKRKLGYYAMPLLWRDRIVGWANISMKNGDLDYQLGYVDEPPLDR
                     TFKRELAAEIERMRGFLGV"
     gene            1188..2144
                     /gene="pip"
                     /locus_tag="VFH96_10505"
     CDS             1188..2144
                     /gene="pip"
                     /locus_tag="VFH96_10505"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET6974446.1"
                     /translation="METLRTLYDAIEPYDSGYIRVSPEHELYYEQCGTPNGKPVVFLH
                     GGPGAGLVADYRRFFDPQAYRVILFEQRGAGRSKPHASLDDNTTWHLVQDIEVIREQF
                     GIDQWLVFGGSWGSTLALAYAETHPERVCGLILRGIFLCRPREITWFYEDSQGASAIF
                     PEIWEQYVRIIPEAERDDMIRAYYKRLTSEHADVRLEAAQAWSNWEASALKLIPDQNL
                     IDEFNEPEMAIALARIECHYFVNNCFFETDNHLIENIDRIRHIPGVIVHGRYDIVCPA
                     MNAWDLHRAWPEAILKIIPDAGHSVTEPGITDALVRATDSFR"
     gene            complement(2249..3196)
                     /locus_tag="VFH96_10510"
     CDS             complement(2249..3196)
                     /locus_tag="VFH96_10510"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6974447.1"
                     /translation="MHKRLSWILTLIFVFIITASAQDKPCPTASKFGPDDQIGNVNYI
                     TPAKTLAATKLVTTGKTYRLAIETNKDTPAYPPRTFAVTIVQPGQTAGGSLGPTKTTY
                     NDDLIVGWAGVGTQLDGLGHIGIDGLYYNCNRASDFAMTDGLKKLGVEHVPAIATRGV
                     LLDMAGYFNTDIVKEGTAFNRAEIEGAMKRQGIKSIEKGDVVLFYTGWLKLIGKDNKR
                     FGSVAPGLGREGAKFLASLDVAIIGTDTWSFEVIPFEKGAGVFEVHQILIPLNGIHIL
                     ENVNTEEMVRDQAWEFLFTLGAARITGGVQSIVNPIAIR"
     gene            complement(3234..4130)
                     /locus_tag="VFH96_10515"
     CDS             complement(3234..4130)
                     /locus_tag="VFH96_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6974448.1"
                     /translation="MEEDLEKTQILNRSQEIPLTEPSEAAAAKTARLPRLDSGAFRIE
                     REKIEEEFRFGQSKILQKLAANAPLSETLERLVLLIEAQAPGMLCSVLLLSEDGDHVR
                     HGAAPSLPAEYVQAIDGSAIGPKHGSCGTAIYRGEPVVVTDILNDPLWEDYRDLAVAN
                     GLRACWSTPILSGRGKVLGSFAMYYREPRTPTGEEAGLTEVATRIAGLAIERHAAKEI
                     LSRTQAELAQASYAASKGKAAASSIDEINQQLEAILDNAKKCLELLDEDKPDIASFRG
                     PLTNITKNGQQALNVIARIRSS"
     gene            4248..>5655
                     /locus_tag="VFH96_10520"
     CDS             4248..>5655
                     /locus_tag="VFH96_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6974449.1"
                     /translation="MNAFGNYINGEFVDSKSGKTFPNVNPANFDEVVGTFQASNEADV
                     NDACSAAVGAQDEWAAMPATKRGEYLFKAAEILASKLPQLGEEMTREEGKTLTEAIGE
                     VTRAINIFRYFGGEGARQFSYNVPSERPQVLCYTIRKPLGTVALITPWNFPSAIPAWK
                     LAPALVAGNTVVLKPASLAPLSSFRLVEALHEANIPKGVVNYVTGSGGAVGEPLTSHS
                     AINGISFTGSTSVGDALYSKVSDRKLRVQLEMGGKNPTIVLRDSDLNYAADILINGAF
                     FSTGQKCTACSRAIIERPIYEPLVQLLIEKTRGLKVGNGLDAGVQIGPAVDANQLETD
                     LEYIKIAEEEGAELLCGGNRLSGGEYDKGYFIEPTIFTGVTPKMRIAQEEVFGPVLGL
                     MVAEDFEDAMEIANGVRFGLSASIVSRDLTRVHQFIDRIEAGLITVNLPTAGVEYQLP
                     FGGTKESSYGMREQGPQAI"
BASE COUNT         1213 a         1538 c         1634 g         1270 t
ORIGIN      
        1 agcctgacgc atggcgatac gtggttgagg tgcaggaccc cggcaagccg gccgatgcga
       61 cgggctaccg gtaaacaatc ctgttaattc tgtaaatcct gatatcttag tccagtgccg
      121 atcacgatcg acgacctgcg acgattcacc atcgcgcgga acttccccaa accaacaaca
      181 ctcaaccgcg ccctgcaaag catgggattc gtgcaggcag atccaattcg cgctcccgca
      241 cgcgcgcagg atctgatcct gcgacatcgc gtcaatgatt atcgcgccgg tgatctggaa
      301 cgccgctaca cctcactcga cgtcgaagaa gatttcttca tcaactacgg ctacgtaaca
      361 cgagcacttc agtccctcat gcatccacgc cctactctgc ggcagagaaa agcaaaactg
      421 ctgctcgatt tcgttcgcga gcgcggcgtt gtacacccgc gtgaggtcga tgagcacttc
      481 gcacacggca ctgtgacaaa ttactggggc ggctcttcaa acgccacgac ccatttgctt
      541 gatgcaatgc actacgcggg catgctccgc atcgtgcgtc gtgagaaagg cattcgggtt
      601 tacgccgagc acaaacacga aaccgtccct cgcaatgcag ccgagcggcg ggcgcggatg
      661 gacgctttag tcgatgttgt ggtccggatt tacgcgccct tgccggggtc aagtctttcg
      721 tattacatga gacgtttgcg atatgccgca ccgcagtggc agaaggagtt gccgagtgca
      781 ttgcaacgtg cgaaagagcg actgtcttgt gcgcgtgtgg ggaaggtcga ttggtactgg
      841 cccgccgctg aaaatatcag cgatcaagcg attgacgatg cggtccgtct cctcgcgccc
      901 ttcgatccgg cagtacacga tcgtaatcgt ttcgagctgt tctggggctg gacttatcgg
      961 ttcgaagcct acacccccgt agccaagcgc aagctcgggt attatgcgat gccgctactg
     1021 tggcgcgacc gcatcgtcgg ctgggccaac atctcgatga aaaatggcga tctcgattac
     1081 caactcggtt acgtcgacga gcctccgctc gatcgcactt tcaaacgtga acttgcggca
     1141 gagatcgaac gcatgcgcgg attcctcggt gtataatcgg tcgcaatatg gaaacactgc
     1201 gaactcttta cgatgcgatt gagccatacg acagcggtta tatccgtgtg tcgcctgagc
     1261 acgagcttta ttacgagcaa tgtggcacgc cgaacggcaa accggttgtc tttctgcatg
     1321 gtgggccggg agcgggactc gtggctgatt accgccgctt cttcgatccg caggcgtatc
     1381 gtgtgatcct gttcgagcaa cgcggggcgg ggcgaagtaa gccgcacgct tcactcgacg
     1441 acaacacgac ctggcatctg gtccaggaca tcgaagtcat tcgtgagcag tttggtatcg
     1501 atcagtggct cgtgtttggc ggctcgtggg gcagcacgct cgcgctcgct tatgctgaga
     1561 cacatccgga acgtgtgtgc gggctcatct tgcgcggcat ctttctgtgc cgtccgcgag
     1621 agattacgtg gttctacgaa gacagtcagg gcgccagcgc gatctttccc gagatctggg
     1681 aacaatatgt gcgcatcatc cccgaagccg aacgtgacga tatgatccgc gcttattaca
     1741 aacgtctgac cagcgaacat gcagatgtgc gcctcgaagc cgcacaggcc tggtccaact
     1801 gggaggcgag tgcgttgaag ctgattcccg atcagaattt gatcgatgaa tttaacgagc
     1861 cggagatggc gattgccctc gcgcgcatcg agtgtcacta cttcgtgaac aactgcttct
     1921 tcgaaaccga caaccatttg attgagaaca tcgatcgcat tcgacacatt cccggagtaa
     1981 tcgtgcatgg tcgttacgac atagtgtgtc ccgcgatgaa tgcctgggat ctacatcgcg
     2041 cctggccgga agcgatactg aagatcattc ccgacgcggg ccactctgtc accgagcccg
     2101 gaatcaccga tgcgctcgtc cgggccacag acagcttcag ataaggttta gccacaaaag
     2161 gcacaaaaag cacaaaaatg attgggagag tcacgcttcg tggtccttcc tacattttgt
     2221 gctttttgtg cctttttgtg gccaaacctt atctgatagc gatggggttg acgattgatt
     2281 gaacgccgcc ggtgatccgt gcggcgccga gcgtgaagag aaattcccac gcctgatctc
     2341 gcaccatctc ttccgtgtta acgttttcga gtatgtggat gccgttcagc ggaatgagta
     2401 tctggtggac ctcgaagacg ccggcgcctt tttcaaacgg aatcacttca aagctccacg
     2461 tgtcggtgcc gatgatcgcg acgtccaacg acgcgagaaa cttcgcgccc tcgcgtccga
     2521 gtccaggagc gactgagccg aagcgtttgt tgtctttgcc gatcaacttt agccaacccg
     2581 tgtagaacaa caccacgtca cccttctcaa tcgacttgat cccctgccgc ttcattgcgc
     2641 cctcgatctc ggcgcgattg aacgcagttc cctctttgac gatatcggtg ttgaagtaac
     2701 cagccatgtc gagcaacaca ccacgcgtcg cgatggcggg cacgtgttcc acgccgagct
     2761 tcttcagacc gtcggtcatc gcaaagtctg aggctcggtt gcagttgtaa tacagaccgt
     2821 cgataccgat gtgaccgagg ccgtcgagct gtgtgccgac gccggcccaa cccacgatca
     2881 ggtcatcgtt gtacgtggtc ttggttggac caagcgagcc gccggcggtc tggccgggtt
     2941 gaacgatcgt gactgcgaaa gtgcgtggtg gatatgcggg cgtgtcttta ttcgtttcga
     3001 tggcgaggcg ataagttttg cctgtcgtga cgagtttcgt ggcagctaat gtcttcgcag
     3061 gcgtgatgta gttgacgttg ccgatttgat cgtcggggcc aaacttcgaa gccgtcggac
     3121 atggtttgtc ctgagctgat gccgtgatga tgaaaacgaa gatgagggtc aaaatccagg
     3181 agagtctttt atgcatagtc agttgttgta caggattagc aagatttaca agattaagaa
     3241 gagcgaatac gggcgatgac gttgagcgcc tgctgtccgt ttttggtgat atttgtaagc
     3301 ggaccacgga aactcgcgat atccggtttg tcttcgtcga gcaactcgag acatttcttc
     3361 gcattgtcca gaatcgcttc gagctgctga ttaatctcat cgatactgga agccgcagcc
     3421 tttcctttgc tcgccgcgta agacgcctgg gccagctcgg cttgcgttcg tgacaggatc
     3481 tccttggcgg catggcgctc gatcgcgagg cctgcgatgc gggttgcgac ttcagttaga
     3541 ccagcctctt caccggtggg tgtgcgcggc tcgcgatagt acatcgcaaa cgaacccaac
     3601 accttcccgc gccctgacaa aatcggggtg gaccagcagg cgcgcagtcc gttagccacc
     3661 gcgagatctc tgtaatcttc ccacagcgga tcgttaagaa tgtcagttac aacaaccggc
     3721 tcaccgcgat atatcgccgt gccacacgaa ccgtgctttg gtccaatcgc cgagccgtcg
     3781 atcgcctgca cgtactccgc cggcagactc ggcgcggccc cgtgacgaac gtgatcgccg
     3841 tcttcgctga gcagcaacac ggaacagagc atgcccggcg cctgggcctc gatcaacagc
     3901 acgagccgct cgagggtctc actgagcggg gcattcgccg ccagtttctg caggatcttg
     3961 ctctggccga aacgaaactc ttcttctatt ttttcacgct cgattcggaa agcgccgcta
     4021 tcgaggcgcg gcagtctggc cgtctttgca gcggcggctt cggatggttc agttagaggg
     4081 atttcctgtg agcggtttag tatctgcgtt ttttccagat cttcttccat ggcttgctcc
     4141 gggcgaaacg tgtgtgacga ggcgcgccta ttttacgccc aaacggcttc caaggcacat
     4201 atgttaggat gcggtgcata ctaagccaaa atcataagga gaacggtgtg aacgcgtttg
     4261 gcaattacat caacggcgag tttgtagaca gcaaatcagg caagactttt ccgaacgtca
     4321 atccggcgaa tttcgacgag gtggtcggca cttttcaggc ttcaaacgaa gcggacgtaa
     4381 atgacgcgtg ttcggcagca gtcggcgcgc aggacgagtg ggccgcgatg ccggcgacga
     4441 agcgtggcga gtatctgttc aaggccgcgg agatactcgc gagtaaactg ccacagctcg
     4501 gcgaagaaat gacgcgcgaa gaaggcaaga cacttactga ggcaatcggc gaagtcacgc
     4561 gcgcgatcaa tatctttcgt tatttcggcg gcgaaggcgc gcggcagttc tcttacaatg
     4621 ttccatcgga gcgcccgcaa gtcctctgct acacgatccg caagccgctc ggaaccgtcg
     4681 cattgatcac accgtggaat tttccgtcag cgattccggc gtggaaactc gcgcccgcgc
     4741 tggttgccgg gaacaccgtc gtactcaagc ccgcctcgct ggctccgctc agctcgtttc
     4801 gactggtcga agcattgcac gaagcgaata ttccgaaagg tgtcgttaac tacgtcacgg
     4861 gcagcggcgg agctgttggc gaaccgttaa ccagtcattc ggcgatcaat ggtatttcgt
     4921 tcaccggctc tacttcagtc ggcgatgcgt tgtatagcaa agtttccgat cgaaaactgc
     4981 gcgtccagct cgagatgggt gggaagaatc cgacgatcgt gttgcgcgat tcggatctga
     5041 attatgcggc tgacattctt atcaacggtg cgttcttctc gactggtcag aagtgtacgg
     5101 cgtgtagtcg cgcgatcatc gagcgtccga tttatgagcc gctggtccaa ctgctgatcg
     5161 agaagacacg cggtttgaaa gttgggaatg ggctcgacgc tggtgttcag atcggtccgg
     5221 cggttgatgc aaatcagctc gaaacggatc tcgaatacat taagatcgcg gaagaagaag
     5281 gggcggagtt gttgtgtggc ggcaacagat tgtcaggcgg tgagtatgac aagggttact
     5341 ttatcgagcc cacgatcttt acgggtgtga ctccgaagat gcggatcgcg caggaagaag
     5401 tgtttggtcc agtgctcgga ctcatggtgg cggaagattt tgaggacgcg atggagattg
     5461 cgaatggagt gcgattcgga ctctcggcgt cgattgtttc gagagatctg acgcgcgtgc
     5521 accagttcat cgaccggatt gaagccgggt taattacagt gaacctgccg actgcgggtg
     5581 ttgagtatca actaccgttc ggcggcacga aggagtcgag ctacggcatg cgcgaacaag
     5641 gaccacaggc gatcg
//
LOCUS       DASSFQ010000291         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1170813, whole genome shotgun sequence.
ACCESSION   DASSFQ010000291 DASSFQ010000000
VERSION     DASSFQ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170813"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2890)
                     /locus_tag="VFH96_10525"
     CDS             complement(<1..>2890)
                     /locus_tag="VFH96_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6974450.1"
                     /translation="LPLDQAAAVLETSASIAAISLRAGIEFLRTAPAAAQVLQSAELR
                     SWGEMGRRLAMADYESAVSFFDEGVDPLKAIAPELRPEVFVLTTRQMVLSTVIGRETL
                     HSLPALATEINDDDLLRSILEVATEIARRSAKHSAEFLNASGTVISELKRFNNADLTA
                     AAIALAGAFASRAGGIASDAWAVIPESLAELSTSDAKRLLAATNDFLERGGGAALHIL
                     IAGGEITRRAPEIFDEWISLLWVVAAHGNASLVAFVRSSPGFIRLLNADPNRAAALEL
                     SRRVLALTKEIAAVDGEAALACFRSASRALRTVSIEQFEAWARRGLTSGRTDSRARRS
                     YFSLETRGSYEALHSGSAGLALDAVQHLLRLYVEALTGREVDVAPLAAVPDEARIDDG
                     RTIHLPSLVNEFGDEELDFRLYKVLAAHAAGQIEFGTYERGGILRVAHSAIAARYAEE
                     NVEALDAFAIADEIVQLRKDGHEEAQKNTKEIGEVGYRDVLKLFPMPQLARRVFGTLE
                     NGRIDRRLRRMYRGLSRDLDLIRQHLTRSRPLMMELPGSLVPFELLFQITMLGGARED
                     ARMAYGQVVSELETIVSDYLVREQSTVADSLMATSRVYDLFQTVIPVDDSVQQVETPE
                     DQTESDDESAVQQMKQRQSERTPERRDARELFNAWNDPSAEGEPDDLMGAEAWSESET
                     PEQVLAEGEVAFNYDEWDRELTDHRLGWCRVVEKKVKHGDRNFVEHTKERHKGVISSI
                     RHQFQLMKPENLIRVANEVDGEEFDLNAVIDYVIDRRADGQQSERIYTKRLRRRRDVA
                     VSFLLDQSSSTARTIGRHPLQPYTRPGRRIIEIEKEGLVLMSEALEAVGDVYSINGFT
                     SEGRRNVKFYVVKDFDEKYNDEVKRRIGGITYQNNTRLGAAIRHATRRLGKQDARTRL
                     LIVLSDGRPYDHDYGDARYAREDTREALRQAKNEGITPFCITIDRESEA"
BASE COUNT          619 a          846 c          798 g          627 t
ORIGIN      
        1 gcctcggatt cacgatcgat cgtgatgcaa aacggtgtga tgccttcatt cttcgcttgc
       61 cgcaaagcct cacgcgtgtc ctcacgtgca taacgcgcat cgccgtaatc gtgatcgtac
      121 ggtcgcccgt cagaaagcac gatgagcaat cgcgtgcgcg cgtcctgttt gcccaatcgc
      181 ctggtggcgt gacgaatcgc agccccgaga cgcgtgttgt tctgataggt aatcccgcca
      241 atgcgtcgtt ttacttcgtc gttatacttc tcatcaaaat cctttacgac gtaaaacttc
      301 acattccgcc gtccctccga agtgaaccca ttgatcgagt agacgtcgcc gacagcttcg
      361 agtgcttcgc tcatcagcac gaggccttct ttctcgatct cgatgattct gcgacctggc
      421 cgcgtgtacg gttgtagcgg atgacggcca atcgtacgcg cagtcgaaga cgattggtct
      481 aacaggaacg atactgccac atcgcgacgt cgccgcagtc gcttcgtgta gatgcgttct
      541 gactgttgac cgtcggcgcg tcgatcgatg acgtaatcaa tcactgcatt cagatcaaat
      601 tcttcgccat caacctcatt agcaacacgg atgaggtttt cgggtttcat cagttgaaac
      661 tgatggcgta tcgacgagat gacacctttg tgtcgttcct ttgtgtgttc gacgaagttg
      721 cgatcgccgt gcttcacttt cttctcgaca actcgacacc agccgagtcg atgatccgtt
      781 aactcgcgat cccactcgtc gtaattgaac gcaacttcac cttcggcgag cacttgctcc
      841 ggcgtctcac tttcggacca cgcttcagcg cccatcaaat cgtcaggctc gccttcggca
      901 gagggatcgt tccaggcatt gaacaactcg cgcgcgtcgc ggcgctcggg agtgcgctca
      961 gactgtcgtt gtttcatttg ctggaccgct gattcgtcgt ctgactcagt ttgatcttca
     1021 ggcgtctcga cctgttgtac agaatcgtca accggtatca cggtttgaaa cagatcgtaa
     1081 acacgactcg tggccatcaa cgagtcggcg actgtcgact gttcgcgcac cagataatcg
     1141 ctcacgatcg tttccaactc agagactact tggccataag ccatccgcgc atcttcccgt
     1201 gcaccgccga gcatcgtgat ctgaaacagc aactcaaaag gaacgagcga cccgggtaac
     1261 tccatcatca gcggccggct gcgagtgaga tgctggcgaa tcaaatcgag atcgcgactt
     1321 agaccacgat acatgcgtcg cagacggcga tcgatacgac cattctcaag cgttccaaat
     1381 acacgccgcg cgagttgtgg catcggaaat agcttcaaca catcgcgata cccgacttcc
     1441 cctatttctt ttgtgttttt ctgtgcctct tcgtggccat cttttcggag ttgcacaatt
     1501 tcatccgcga tggcgaacgc atcgagtgct tcgacgtttt cttccgcata gcgtgcagcg
     1561 atggcggaat gggccacgcg caagatgccg ccccgttcgt acgtgccaaa ctcaatctgt
     1621 ccggcagcgt gcgcggcgag cactttgtaa agcctgaaat ccagctcttc gtcgccaaat
     1681 tcattcacaa gcgaaggcaa gtgaatcgtg cgaccatcgt cgatgcgtgc ctcgtcagga
     1741 accgccgcga gcggcgcaac gtcgacttcg cgtcccgtga gcgcctcgac atacaaacgc
     1801 agcagatgtt ggaccgcatc gagtgccaga ccggcgctgc cgctatgcaa tgcttcgtaa
     1861 ctgccacgcg tttcgagcga gaaataactg cgacgcgcac gcgaatcggt gcgaccagac
     1921 gtgagcccgc gacgtgccca ggcttcaaat tgttcgatgg aaaccgtgcg caacgctcgt
     1981 gacgcagaac gaaaacaggc gagcgcggct tcgccgtcga ctgcggcgat ctcttttgtg
     2041 agcgcgagaa cgcgccgcga gagctcgagt gcagcggcgc gattcggatc ggcattcagc
     2101 agtcgaatga atccgggact gctacgaaca aaagccacga ggctcgcatt gccgtgtgcc
     2161 gcgactaccc agagcaacga gatccactca tcaaaaatct caggcgcgcg ccgtgtgatt
     2221 tcgccaccgg caatcaagat gtggagcgct gcaccgccgc cgcgttcgag aaagtcgttg
     2281 gtcgctgcga gtaaacgctt cgcgtcagac gtcgagagtt cagccaggct ttctggaatc
     2341 acagcccacg cgtcagaggc gatgcctccc gcgcgagatg caaacgcacc ggcgagcgca
     2401 atcgctgcgg cagtcagatc agcgttgttg aaacgtttga gttcggagat gacggtgccg
     2461 ctggcgttga gaaactcggc gctatgtttt gccgagcgac gcgcgatctc ggtggcgact
     2521 tcgagtatcg agcgcagcag atcgtcatcg ttaatctctg tggcgagagc gggcaatgag
     2581 tggagggtct cgcgaccaat gacagtcgag agaaccatct gtcgagtggt taagacgaag
     2641 acttcgggac gaagttcagg cgcgattgct ttgagtggat cgacgccttc gtcgaagaaa
     2701 gaaacggctg actcgtagtc agccattgcg agacgccggc ccatctcgcc ccacgagcgc
     2761 aactctgctg attgtagaac ctgcgcggca gccggcgctg tgcgtagaaa ttcgatgccg
     2821 gcacgtagtg agatggcggc gatcgaggca ctggtttcga gcaccgcagc cgcctggtcc
     2881 aacggcaagc
//
LOCUS       DASSFQ010000292         5691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1170894, whole genome shotgun sequence.
ACCESSION   DASSFQ010000292 DASSFQ010000000
VERSION     DASSFQ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170894"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFH96_10530"
     CDS             complement(<1..468)
                     /locus_tag="VFH96_10530"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6974451.1"
                     /translation="MPLRGKKVLIVGAARSGIGAAKFLVAQGAVVALTDQKPIEKWTP
                     EALALKELGVGLLPGEPPSWLLDQLDLVVVSPGVPATIIPIRYAERAGAEVIGEVELA
                     YRYLKGRVVAITGSNGKTTTTSLIGELLRDAGMTVQVGGNIGKALISMIETSRD"
     gene            complement(486..1640)
                     /gene="mraY"
                     /locus_tag="VFH96_10535"
     CDS             complement(486..1640)
                     /gene="mraY"
                     /locus_tag="VFH96_10535"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET6974452.1"
                     /translation="MIYYFLYELIYVHFKDKDWYLVKALNVFQYVTFRTAYATITALL
                     ISLFFGGKLIEFLKEWKVGQQIREEGPQAHQVKRGTPTMGGVLIVASVLISTLLWSRL
                     TSVYAWTAMIATLGFAAIGFIDDYAKMAKQRSLGLTGRQKLLAQLLIAVGVWAVLFTA
                     TKLIGGTGYSWNLSIPFLKWTADPNKSFVGPYIYLVLIVVVLLGSSNAVNLTDGLDGL
                     AISVTFIAMSALTGFTYLSSDARWAGYLELTHRPEAAELTVFCGAMAGASLGFLWYNA
                     PPAQVFMGDVGSLAIGGAMGTVAVLTKQEFMLLMVGGVFVIEALSVMLQVGVFKMTRR
                     TTGTGRRLFKMTPLHHHFEMQDWKESKIVFRFLILAILFALLSLSTLKLR"
     gene            complement(1713..3182)
                     /gene="murF"
                     /locus_tag="VFH96_10540"
     CDS             complement(1713..3182)
                     /gene="murF"
                     /locus_tag="VFH96_10540"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET6974453.1"
                     /translation="MKVGEAAALMGANASSISAEMFDKQIKDYSIDSRSVGAGELFFA
                     LSQQDYARAGFNGEFVDAHRFIGDAFERGAIAAVARTDRVSGDAYLESVKDRLLLVDD
                     AIAALQLLAHRVYTNWGKPVVAITGSAGKTTAKELTAHVLKSAGHRVLKSERNYNNGL
                     GLPLSVLRMVSEDRSPDQFDLAVLEMGMSSPTHEIQRLCKITPPDVAVELMVAPVHLE
                     YLGTIENIAAAKAELIEGLKPEGTAILNADDALVMKMRDKHSGRTLTFGIDNVADISA
                     SEIDTCSLGRIEFRLRTPLGESRALLHMSGRHNLSNALAASAVGTAFSIPPEEIAAAL
                     QTAQPPKMRGEVFDFAAGFTVIDDSYNSNPRSLLNMVRTMAEGGRGRKRLIVIAGEML
                     ELGPDEVSLHRDVGREIAGSGIDVLWGVRGLGKEIVAGANEAGLATTRFFDSSDEVAE
                     QLMREVKEGDLILVKGSRGVATDKIVKALRERFPLAGAE"
     gene            complement(3271..3453)
                     /locus_tag="VFH96_10545"
     CDS             complement(3271..3453)
                     /locus_tag="VFH96_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974454.1"
                     /translation="MKQLSKMTYTTALLTADDDYAASVHPSGHKIIPQNMGRAVMCVL
                     PRVVLEALPRFWQTFR"
     gene            complement(3471..5558)
                     /locus_tag="VFH96_10550"
     CDS             complement(3471..5558)
                     /locus_tag="VFH96_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET6974455.1"
                     /translation="MLARLVARSKSRPDVSPGRALFVAAFIGFWMLVVCARLVYLQFS
                     QYDVLASRARQQQQNEIETMPQRGELLDRQERQLARSVQTVSLFIDPDGLDTATLDRN
                     AQQIAQQLARPLNLKQSQLAADFRKAHDDKKRFVWIARRLDADLANKVVALNLPGVQT
                     QLEPKRYYPNGSLAAHVLGYVGLDGKGLGGVEQFFNAKISGEPGQLFLEKDANGKPYE
                     GYEIAAKPGQTVVLTIDQTIQYQAEQALQAAVQQSHAKSGSVIVLDPRSGEILALANA
                     PTFDPNKVSEAKPETRSNWALQNIYEPGSTFKVVAFSAALEKGLARVDERIDCQMGSI
                     TVAGRLVHDHKPFGLLTIPEALAKSSNVAAIKLGIRVGDPTMYEYIRRFGFGAKTGIE
                     LPGETNGILRKVERWQPSSIGSIAIGQEVGVTPVQMVAAFGALANDGMRIAPHLIREV
                     RDAGGNVVYRAQPEQHRVISAETAIALRGMLEGVTLNGTAKKAQLDGYSAAGKTGTAQ
                     KIDPRTKAYSSTKFVGSFVGFAPVSNPQVVIIVVIDEPAGAYHGGDVAAPVFREVAEQ
                     VLPTLGIEPDIETKSAPDLIAQQINENPERAEKIREEQAQSEQQRKATMPTVDSNGGR
                     SGEVVYAASTRKAILMPDLRGRSVRDVARTCAQLGLQVEARGEGKVFKQSPSAGTEVS
                     TGQLIYVDFGRSQ"
BASE COUNT         1225 a         1765 c         1443 g         1258 t
ORIGIN      
        1 gtcacgtgaa gtctcaatca tcgagatcag cgctttaccg atgttgccgc cgacctgaac
       61 cgtcataccc gcatcgcgca acaactcacc gatgagcgac gttgttgttg tcttgccgtt
      121 actgcccgtg atggcaacga cgcgaccttt caaataacga tacgcgagct cgacttcacc
      181 gatgacttcc gcgcctgctc gctcggcgta acgaatcgga ataatcgtcg ccggaacacc
      241 tggactgaca accacgagat caagttgatc gagcaaccag ctcggcggct cgcccggcaa
      301 caaacccacg cccaactctt tcagtgccaa agcctcaggc gtccatttct cgatcggctt
      361 ctgatcggtg agcgcgacga ccgcgccctg ggccacaagg aacttagctg caccaatccc
      421 actgcgcgcc gcgccaacga tcaaaacttt tttgccacga agaggcacaa aaaccacaaa
      481 aacgattacc gcaatttcaa agtcgataga ctcaataagg cgaagagaat tgccaatatc
      541 aaaaaccgga agacgatctt cgactccttc cagtcctgca tctcgaagtg gtggtgcaac
      601 ggcgtcatct tgaataaacg tcgcccggtg ccggtcgtgc gtctcgtcat cttgaacacg
      661 ccgacctgca acatcaccga caacgcctca atcacaaaca cgccgccgac catcagcaac
      721 atgaactcct gcttggtcag caccgccacc gttcccatcg cgccgccgat cgcgagactg
      781 ccgacgtcgc ccatgaacac ctgcgcgggc ggcgcgttgt accagagaaa cccgaggctt
      841 gctcctgcca tcgcaccgca aaagaccgtc agctctgctg cttccggccg gtgcgtcaac
      901 tcgagatatc cggcccagcg agcgtcgctg ctcaggtaag taaaacccgt caacgcgctc
      961 atcgcgataa acgtgacgct gatcgcgagt ccatccaaac catctgtcag gttcactgcg
     1021 ttcgacgatc cgagcaacac cacgacgatc agcaccaggt atatataagg accgacaaaa
     1081 cttttgtttg gatcagccgt ccatttcaaa aacgggatgc tcaggttcca cgaataaccg
     1141 gtcccgccaa tcaacttcgt cgccgtgaag agcacggccc aaacgccgac tgcaatgagc
     1201 aactgcgcga gcaacttctg ccggcccgtg agcccgagac tgcgctgctt cgccatcttc
     1261 gcgtagtcgt cgatgaaacc gatcgctgcg aagcccagtg tcgcgatcat cgcggtccac
     1321 gcgtaaacgc tggtgagtcg cgaccacagc agcgtcgaga tcaaaaccga agcgacgatc
     1381 agcacgccgc ccatcgtcgg tgtgccgcgt ttgacctggt gcgcctgcgg gccttcttcg
     1441 cgaatctgct ggcccacctt ccactctttc aaaaattcaa tgagcttgcc gccgaagaac
     1501 agcgagatca gcagcgccgt gatcgtcgcg tacgcagtac gaaacgtgac gtactgaaag
     1561 acgttgagcg cctttacgag ataccaatcc ttgtccttga aatggacgta gatgagttcg
     1621 tacaagaagt aatagatcat cgagtttccc tatccacaga ttacacagat agatctattg
     1681 aatctgtgta gtctgtggac aaaacttctt ctttattccg ctcccgccaa cggaaaacgc
     1741 tcacgcaacg ctttcacgat cttatccgtc gctactccgc gcgatccttt caccaaaatc
     1801 agatcgcctt ctttcacttc tctcatcaac tgctcagcga cttcatctga cgagtcgaaa
     1861 aaccttgtcg tcgcgagtcc cgcctcattc gcacccgcaa cgatctcttt acccaaacca
     1921 cgaacacccc acagcacatc gatcccgctg cccgcgatct ctctgccaac atcacggtgc
     1981 aacgacacct cgtcaggtcc gagctcgagc atctcacccg cgatcacaat cagtcgcttg
     2041 cgcccgcggc caccttccgc catcgtccgc accatgttca gcaacgagcg cgggtttgag
     2101 ttgtaagagt cgtcaatcac cgtgaagccc gccgcaaaat caaaaacctc accacgcatc
     2161 ttcggcggct gcgctgtctg caacgccgcc gcaatctctt ccggtggtat tgagaacgct
     2221 gttcccacag ccgacgctgc gagcgcgtta ctcaagttgt gacgaccact catatgaagc
     2281 aacgcacgcg attcacccaa cggagttcgc aaacgaaact caattcttcc gagcgagcaa
     2341 gtatcgatct cgctcgcgga aatgtccgca acgttgtcga taccaaacgt caacgtgcgg
     2401 ccgctgtgct tgtcgcgcat cttcatcacg agcgcgtcgt cggcgttgag aatcgcagtg
     2461 ccttcaggtt tcaatccttc gatcaactcc gcctttgcag ccgcgatgtt ttcgatcgtg
     2521 ccgagatatt cgagatgaac cggcgcaacc attagctcca ccgcaacatc gggcggcgtg
     2581 atcttgcaga gcctttgaat ctcgtgcgtc ggcgacgaca ttcccatctc gagtaccgcg
     2641 agatcaaact ggtccggtga acgatcctcg ctaaccatgc gcaagactga cagcggcaat
     2701 cccagtccat tgttgtaatt ccgctcgctc tttaacacgc gatgcccagc gcttttcaaa
     2761 acatgtgcag tcaactcttt cgccgtcgtc tttccggcac tgccagtgat cgcgacaact
     2821 ggttttcccc agtttgtgta aacacgatgc gcgagcagtt gtaacgctgc gatcgcatcg
     2881 tcaaccagca gcaaacgatc tttcacgctc tcgagataag catcgccgct cacgcgatcg
     2941 gtgcgcgcaa ccgccgcgat cgcaccacgc tcaaatgcgt cgccgataaa acgatgcgca
     3001 tcgacaaact caccattaaa accagcgcgg gcgtagtcct gttgcgagag cgcaaagaag
     3061 agctcgcccg cgcctaccga acgcgagtcg atggaataat cttttatctg tttgtcaaac
     3121 atctcggcgc tgatgctcga tgcatttgcg cccatcaaag ctgctgcctc gccaaccttc
     3181 acgtgattac ggcctcttcc ttttcttcct gcgaggacgt gtcagccgaa tcgagcaagc
     3241 attcaactag ccttgaaagc gagctctggc tcatcgaaac gtttgccaaa atctcggcaa
     3301 agcttccaac acgacccgcg gcaggacgca cataaccgct cgacccatgt tttgggggat
     3361 gatcttgtgc ccggagggat gaacgctggc tgcgtagtcg tcgtctgcag tcagcaatgc
     3421 cgtagtgtac gtcatcttcg agagttgttt catctgtcgg gtttaacaac ttactgcgac
     3481 ctcccaaaat caacgtaaat aagctggcct gtgcttactt ctgtgccggc tgacggactc
     3541 tgtttgaaaa cttttccttc gccgcgtgct tcaacctgca aaccgagctg cgcgcacgtt
     3601 cgcgccacat cacgcacgct gcgtccccgt aaatctggca tcagtatcgc cttgcgtgtc
     3661 gaggcagcgt agacaacttc accgcttcgg ccgccgttgc tgtccacggt tggcattgtg
     3721 gctttccgct gctgttccga ctgcgcctgt tcttcgcgaa tcttctcggc gcgttcggga
     3781 ttttcgttga tctgttgcgc gatcaaatcg ggggcggact tcgtctcgat gtcgggctcg
     3841 atgccgaggg tcggcagcac ttgttcggca acttcacgaa acaccggcgc ggcgacgtcg
     3901 ccgccgtgat acgcaccggc cggttcgtcg atcacgacga tgatgacgac ctgcggattg
     3961 ctcaccggcg cgaagccgac aaacgagccg acaaacttcg tcgacgaata agctttcgtt
     4021 cttggatcga tcttctgtgc ggtgccggtt ttgcccgcgg ctgagtaacc gtcgagctgc
     4081 gctttcttcg ccgttccgtt cagcgttacg ccttcgagca ttcctcttaa cgcgatcgcg
     4141 gtctcagcac tgatcacgcg gtgttgttcc ggttgtgcac gatagacaac gttaccgccg
     4201 gcatcacgaa cttcacggat caagtgcggc gcgatacgca tgccgtcgtt ggcgagtgcg
     4261 ccaaatgctg caaccatctg taccggcgtc acaccaactt cctgcccgat cgcgatcgag
     4321 ccgatcgacg acggctgcca gcgctcaacc tttcgcagaa ttccgttcgt ctcgccgggc
     4381 aattcgattc ctgtctttgc gccgaagccg aaacgacgga tgtattcata catcgttggg
     4441 tcgccgaccc tgatcccgag cttgattgca gcgacgttgc tcgacttcgc gagcgcctcg
     4501 gggatcgtta acaaaccaaa tggcttgtga tcgtggacca ggcgtcccgc aacagtgatc
     4561 gagcccatct gacaatcgat ccgttcatca actcgcgcga gccccttctc caacgctgcg
     4621 gagaacgcga cgactttgaa cgtcgatccc ggctcgtaga tgttttgcag cgcccagttt
     4681 gatctcgttt ccggtttggc ttcagagact ttgtttggat cgaatgtcgg cgcatttgcg
     4741 agcgcgagga tctcgccgct gcgtggatcg agcacgatca cgctgccgga tttcgcgtgc
     4801 gattgctgca ccgctgcttg caacgcctgt tcggcctggt actgaatcgt ttgatcgatt
     4861 gtcagcacaa ctgtctgacc gggctttgca gcgatctcgt aaccttcgta cggcttgccg
     4921 ttcgcgtctt tctcgagaaa tagctgacca ggctcaccag agatcttcgc gttgaaaaac
     4981 tgttcgacac cgccgagacc tttgccgtcg agtccgacgt aaccgagcac gtgtgccgcg
     5041 agtgatccgt tcgggtagta acgcttcggc tcgagctgtg tttgcacgcc cggaagattg
     5101 agggcgacga ctttgttcgc cagatctgcg tcgagtcgac gcgcgatcca gacgaagcgt
     5161 ttcttatcgt cgtgcgcttt gcgaaagtca gcagcgagtt gcgactgctt cagattgagt
     5221 ggccgagcta gttgttgggc gatttgttgc gcgttgcgat cgagagtcgc ggtgtcgaga
     5281 ccatccggat cgataaacaa cgagactgtc tgcacgctgc gtgcgagctg gcgttcctgg
     5341 cggtcgagca gctcgccgcg ctgtggcatt gtctcaattt cattttgctg ttgttggcgc
     5401 gcgcggctcg ccagcacgtc gtattgcgag aactggagat agaccagtcg cgcgcaaaca
     5461 acgagcatcc aaaaaccaat aaaagccgcg acgaaaagcg cgcggcctgg cgagacatca
     5521 ggtctggact ttgatcgagc gacgaggcgt gccaacatca ccttgtttcc ttctacccag
     5581 cagcgtgatg aggacgcttt tatttagcca cggattaaac ggatttcact gtcagtgttc
     5641 atctgtggct atttccaatt tgccctggcc gcgcaggctt taatccgagg c
//
LOCUS       DASSFQ010000293         9134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_739603, whole genome shotgun sequence.
ACCESSION   DASSFQ010000293 DASSFQ010000000
VERSION     DASSFQ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739603"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFH96_10555"
     CDS             <1..182
                     /locus_tag="VFH96_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974456.1"
                     /translation="AAANAATTKLLRDRTRLESEIEALIGNKSRMKEAEYFVELEKLF
                     VELAKVNRSIKQAGS"
     gene            251..2866
                     /locus_tag="VFH96_10560"
     CDS             251..2866
                     /locus_tag="VFH96_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974457.1"
                     /translation="MRFLLQLCAFACTILLIALSAGAQSTDQRGLAELKKGDYENASK
                     LLNARLVSNPNDAVALRALLRVFIETGRYAEAEATAKRFLLKTPDSGSVRHELAETLA
                     LTGRYSEAITEFERAAADSAKANATADKLESDLRRAEILDLIGQEDNARTIYATFVKH
                     YTDNDPRSARELTLVARALVHLERYQDANDMYRSAIEADSDYLEAQLGAGELFTEKYA
                     YGDAALFLEDAFKINPNSARAYLDLARNKRLDGDAETQAAIAKALAINPNLVDALALK
                     AAISLEASQLNEAATEIDKALKINPRSLEAHSLKAAMLYLQDKDYEPEVAATLAIGPK
                     YGSVYNTLSHYATITRRSEQAAQFARRAIEIAPRLWDAHLNLGMSLLRLGQMEPGREA
                     IEKAFKGDPFNVWAKNTLDLLDTMREYKETKTGPFIIKASAQESDVLSPYAANLLEEA
                     AAKLTSKYKFTPKGPIIIEIFQNHEDFAVRTLGIPGLGALGVCFGFVVAQDSPSAREA
                     GEFNWGSTLWHEYTHVITLQMTDYRIPRWFSEGLSVYEERRARPGWGDDWNPLFVRAF
                     MERRWFRMADLDAGFMRPKSPQDVPVAYFQASQVCEFIVEKYGFDAILRMLAMYRDKA
                     HTPEILQQVLKLTESDFDREFAAYIETKVRPLQHALATQNNIAASLSKEEVLKLLATQ
                     DTFALRIRAAELLAADGNTEAAVAHYIRALELFPYVTGAGNPYESLAKLLEQKGDRAQ
                     AARILDNLVKTDENNLEALKTIARIRLALGEQRPALDALQASFYINPFDYKLHTQAGE
                     LSVELKDYGKALTEFQVALALAPPNVAEANYNVAAAYHALGRQPEAKRAVLRALEAAP
                     RYEKAQELLLRIVGQ"
     gene            2898..3917
                     /locus_tag="VFH96_10565"
     CDS             2898..3917
                     /locus_tag="VFH96_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6974458.1"
                     /translation="MAVEQLTISDEQLLDKLGTARERLLREIRKAIIGQDEVVEQVLL
                     ALFVGGHSIITGVPGLAKTLLVRTIANVLDLSFKRIQFTPDLMPADITGTEIIEEDRA
                     TGHRAMQFIPGPIFANIVLADEINRTPPKTQAALLEAMQEGRVTVQGVSYDLPRPFHV
                     LATQNPIEMEGTYPLPEAQLDRFMFNVKIGYLPEEEEVAVVKQTTSPQDYDFQRLMSA
                     DEIIAFQRLVRQVPASDTVTRYAVRLVNASRPGTPPAPDFVNKWVTWGASLRASQFLI
                     FGGKARAILRGRYNVSCEDVRAVAPAVLRHRVLLNFQAEAEKVDSDLVIKRLIETVPE
                     PKSGL"
     gene            3941..4846
                     /locus_tag="VFH96_10570"
     CDS             3941..4846
                     /locus_tag="VFH96_10570"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6974459.1"
                     /translation="MQPATTPARFLDPELLGRIGSLELLARAVVEGFMSGLHRSPYTG
                     FSTEFTEYRQYNPGDDLRYLDWRLLGRTDRYFIKKYRADTNTQCHLLIDTSASMNYAH
                     ASSVTKLQYTQFLAASLAYLLNRQQDAVGLVAFAEKVHTHVPARNRTGHMRTIFGNLS
                     MLQAGGQTHLAESLHQLAEILTRRGIVVIISDFYDQPDRLQESFQHLRFKGHDLVAFQ
                     VLDQNELDFDFTDPVLLLEDAETQEQMPVLPDVVMSGYRERMRKHVDEMRGCAAANNV
                     DFEMLTTKQPLDFALFSFLSRRAGK"
     gene            4852..6900
                     /locus_tag="VFH96_10575"
     CDS             4852..6900
                     /locus_tag="VFH96_10575"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HET6974460.1"
                     /translation="MSFLTPLFFLGVAALAAPILVHLVRRTRARRVQFPALVFVRQVP
                     QRTIRRRTLHNILLMLLRCLAILLIVIAFTRPFFSGGSAAKDNTAAGATVILVDNSLS
                     MRREGLFAEAQRRAESAVDDARNDEQIALMSFDKRYTVINRFLTDKNRVRTGITSLAA
                     GWDGTDYEQALRGAESLLSEVETTGPKRIVMISDFQAPGWTQASATFKLSNSTQLTTL
                     DVGGNNPPANIAITNVEARGVVFGQKYLDNLVVHLSNFSDTPKDHISVDFQINDQTVE
                     KRDISLNSRDSRVVEFTGFNLNDGANRCTIDIGTGDFAPDNRFYFTLRRETPAKALIV
                     EGASRGRSDSLHLQSALTADDELPFAFTLKSAGSIDPSSVSEYSLVVLNDAGPISSAL
                     ADTLVRFVNAGGQMIVSTGPKTQADTFNQSLQTILPAVLREQVQTKAGESVAITEVKF
                     DHPIFEVFQSSGRLASANVIGYFRSEPRANSSVLARFEDGSPALVEASTGKGRVLLFT
                     SSLGPSWNDLPLTPLYLPFIHQMVRYAGSREESAWSSLGETFTVSKRQDPAPPAVDTP
                     GGVRLSETRLTPDGDLLVTAREPGFYRLRYNSAPDFAGVNIDGAEGDFTKLNFGEFMA
                     GVTGGAGSAEGANANRNASGEEVEGRQRVWWLLLLIALLLLLAESFLARRTKMVKMIG
                     "
     gene            6947..>9134
                     /locus_tag="VFH96_10580"
     CDS             6947..>9134
                     /locus_tag="VFH96_10580"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HET6974461.1"
                     /translation="MTPETNLLQDLLKRARSRRQLLISLRGVAITVGVVAALLLLTGW
                     TAHRFRYNGWALITLRVGALLMVLATIYFALLRPLLKRISDARLARLIEEHSPGTEDR
                     LVTAVEYSDGNRSHISPAIVSRLYQDANSLSGMLDVGNVIRRSRLLLYGGAALASLLV
                     FAGVLKFGPKEISQGVAQLVTPTTLAASTNAMSIKVKPGTARVPKGSDQDIVATLVNF
                     DSQQVTVFARPLGSKNDFQGQLMEPAKAKTDFRFSIFNIQDSMEYFVESNNIRSEVFK
                     LNVVDLPFVKQLDLTLNFPAFTNLPTKTVEDGGDIAALKGTVATITAKLTGKVRAAHI
                     VFPDGKKTEMHLQGSDFVGQLNVTGDTSYYIELVSVDGEAYRGSNEYDVTVLGDQPPV
                     ISFDKPGRDRKATNLEEVFTQARAEDDYGVVSMDLHFSVNGGEEKTVNLQQLSRESAR
                     TLTGAYTFFLEEYNLKPGDFISYFAKARDASNEATSDIYFIEVKPFEMEYKQSQQGGG
                     GQGQGGDQDQNALSRRQKDLIAATHKLLREGAKYTDQERKDGYEAVAAGQEKLRTDTL
                     EFLDRMGRRLGEIDGQKQVQEMAEHLKQAAKEMEGAPPPLRKEAGKDALPPEQRALQR
                     LLAADAIFREVQVAFGNQNSSGSGGGEREQQELAGLFELELDKMKNQYETVQRAQQQQ
                     AEQQKSEAERRLEELARRQQQALEEQRRRQQQSGGGGGGNQRQQQEL"
BASE COUNT         2082 a         2580 c         2563 g         1909 t
ORIGIN      
        1 aagctgcggc taatgctgcg acaacaaaac tgcttcgtga tcgcacgcga cttgaaagtg
       61 agattgaggc tctgattggt aacaagagtc gaatgaagga agctgagtat tttgttgagc
      121 tggaaaagtt atttgttgaa ctggcaaaag tgaatcggtc gatcaagcaa gctgggtcat
      181 gatgaagaac tttcacgcaa aggcgcaaag acgaagaaaa gaacgcaaag aaaacgtagg
      241 gcgaccctct ttgcgctttc ttttacaact ttgcgccttt gcgtgcacaa ttctgctcat
      301 cgccctgagc gccggggccc aatccacgga ccagcgtgga ctcgcggaac tcaaaaaagg
      361 tgactacgaa aacgcttcca aactgctaaa cgcccggctc gtttcgaacc ccaacgatgc
      421 cgtggccctg cgagcgttgc tgcgcgtctt tatcgaaacc ggccgctacg ctgaagccga
      481 agcaaccgcc aaacgctttc tcctgaaaac tcccgacagc ggctcagtcc gtcatgagct
      541 tgccgaaaca ctcgccctca cgggtcgcta ttccgaagca atcaccgaat ttgaacgcgc
      601 tgccgccgat tccgccaaag ctaacgccac cgccgataaa ctcgaaagcg atctgcgtcg
      661 tgccgaaata ctcgatctca tcggacagga agataacgcc agaacaatct acgcaacttt
      721 cgtcaaacac tacactgaca acgatccgcg gagtgctcgc gaactaaccc tcgtcgcgcg
      781 tgccctcgtc catctcgagc gctaccagga cgccaacgac atgtatcgct cggcgatcga
      841 agccgattca gattacctcg aagcgcagct tggcgccggc gagttgttca ccgaaaaata
      901 tgcgtacggc gacgccgcac tgtttctcga ggatgctttc aagatcaatc caaacagcgc
      961 tcgcgcctat ctcgatctcg cgcgtaacaa gcgtctggac ggcgacgccg aaacacaagc
     1021 ggccatcgcg aaagctctcg cgatcaatcc caatctcgtc gacgcactcg cgctcaaggc
     1081 cgcgatatca ctcgaagcca gccagctcaa cgaagccgca accgaaatag ataaagcact
     1141 caagatcaat ccgcgttcac ttgaagcaca ttcgctaaag gctgcgatgc tttaccttca
     1201 ggacaaggac tatgaaccgg aagtcgcagc cacgctcgcg atcggtccaa agtacggcag
     1261 cgtttacaac actctttcgc attacgccac cataacgcgt cgctccgaac aagccgcgca
     1321 gttcgcccga cgagccattg agatcgcgcc acggttgtgg gacgcacatc tcaacctggg
     1381 catgtcgctg ctgcggctgg gccagatgga accaggtcgt gaagcaatcg agaaagcttt
     1441 caaaggtgat ccgttcaacg tctgggccaa gaacacgctc gacctgctcg acacgatgcg
     1501 tgagtacaaa gagacaaaga ccggcccgtt cattatcaaa gcttcggcac aggaaagtga
     1561 cgtgctttcg ccgtatgccg caaacctgct tgaagaagcg gccgcgaagc tgacgtcgaa
     1621 gtataagttc acacccaaag gtccgatcat catcgagatc tttcagaatc acgaagactt
     1681 cgctgtgcgt acactcggca ttccgggact cggcgcgctc ggagtttgtt ttggatttgt
     1741 cgtcgcgcag gattcacctt cggcgcgtga ggcgggagag ttcaactggg gcagcacact
     1801 ctggcacgaa tacacccacg tcatcacctt gcagatgact gactaccgca ttccgcgctg
     1861 gttctcggaa ggtctttcgg tttacgaaga gcgacgtgcg cgacctggtt ggggcgacga
     1921 ttggaatcca ttgttcgtgc gcgcattcat ggagcgccgc tggttccgca tggcggatct
     1981 cgacgcgggc ttcatgcgtc cgaagagtcc gcaggatgtt ccggttgctt atttccaggc
     2041 gtcgcaggtt tgtgagttta tcgtcgagaa gtatggcttt gacgcgatcc ttcgcatgct
     2101 ggcgatgtat cgcgacaagg cgcacacgcc ggaaatactg caacaggttt tgaaacttac
     2161 cgagagtgat ttcgatcgcg agttcgctgc ttacatcgaa accaaagtta gaccactgca
     2221 acatgctctg gccacgcaaa acaacatcgc ggcgtcactc tcgaaagaag aagtgctcaa
     2281 gctgctggcg acgcaggaca cgtttgccct gcgtattcgt gcggctgagt tgctcgcggc
     2341 agacggcaac acggaagcgg ctgtcgcgca ctacattcgc gctctcgagc tgtttccata
     2401 tgtcactgga gctggaaatc cgtacgagtc gctcgcgaag ctgctcgaac aaaaaggcga
     2461 tcgcgcccag gccgcgagga tcctggataa tcttgttaag actgacgaga acaatctcga
     2521 agcgttgaaa acaattgccc gcattcgtct cgcactcggc gaacagcgac cggcactcga
     2581 tgccctgcaa gccagctttt acatcaatcc atttgattac aagttgcaca cgcaagcagg
     2641 cgagttgagt gtcgagttaa aagattatgg aaaggcgttg acggagtttc aggtggccct
     2701 ggcactggcg ccgccgaacg tggcggaagc aaactacaat gtggccgcgg cctatcacgc
     2761 gctgggtcgc cagccggaag caaagcgcgc cgtgctgcgc gcacttgaag ccgcaccgcg
     2821 ttacgaaaaa gctcaggagt tgttgttaag aatcgtagga cagtaatctg tgtaatctgt
     2881 ggatggatgg ggaaaacatg gcggttgaac agttaactat ttccgacgag caactgctcg
     2941 acaaactcgg caccgcgcgc gagcgtctgc tgcgcgaaat cagaaaggcg atcatcggac
     3001 aagatgaagt cgtcgaacag gtcctgctcg cactcttcgt cggcggccat tcgatcatca
     3061 ccggcgttcc cggtctcgca aagaccctgc tcgtgcggac catcgcgaat gtgctcgacc
     3121 tctcgttcaa gcgaatccag ttcacgcctg atttgatgcc tgccgacatc accggcaccg
     3181 agatcatcga ggaagatcgc gccactggtc atcgcgccat gcagtttatt cctggtccaa
     3241 tcttcgccaa catcgtcctg gctgatgaga tcaatcgcac gccgccaaag acccaggccg
     3301 ccctgctcga agcaatgcag gaaggtcggg tgacggtgca gggtgttagt tacgatctgc
     3361 cgcgaccgtt tcatgtgctg gctacgcaga atccgatcga gatggaaggt acgtatccac
     3421 tgcccgaagc gcagctcgat cgttttatgt tcaatgtgaa gattggatat ttgcctgaag
     3481 aggaggaagt cgccgtcgta aaacagacga cgtcgccaca ggattacgat ttccaaaggt
     3541 tgatgtcggc tgatgagatc attgcgtttc agagacttgt ccggcaggtg cctgcgtctg
     3601 acacggtcac gcgttatgcg gtgcggttgg ttaacgcgag ccgtcccgga acgccacctg
     3661 cgcccgactt tgtgaacaag tgggtaacgt ggggcgcgtc gctgcgtgcc tcgcagtttt
     3721 tgatctttgg cggcaaagca cgggcgatac tgcgtggtcg atacaacgtt tcgtgtgaag
     3781 acgtgcgcgc cgttgcgccg gcagtgctgc gacaccgcgt gttgcttaac ttccaggctg
     3841 aagcggagaa ggtcgattcc gaccttgtga tcaagagatt gattgaaacg gtacctgagc
     3901 cgaagagtgg tttgtagaca ggatcctgtc caaggttttt atgcagccag caacgacacc
     3961 agcgcgattt ctcgatcccg agttgctcgg acgcatcggc tcgctcgagt tgctggcgcg
     4021 tgcggtggtt gaaggattta tgtccggctt gcatcgctcg ccgtacaccg gtttctcgac
     4081 ggagtttacc gagtatcgac agtacaatcc cggcgacgat ctgcgttacc tcgactggcg
     4141 gctactcgga cgcaccgatc gttacttcat caagaaatac cgtgccgaca cgaacacgca
     4201 gtgtcatttg ctcatcgata ccagcgcctc gatgaactac gcgcacgcaa gctcggtaac
     4261 aaaattacag tacacgcagt ttctggcggc gtcgctggcg tacttgttga atcggcagca
     4321 ggacgcagtc ggactggttg cttttgcaga gaaggttcac acgcacgttc cggcgcgcaa
     4381 tcgcaccgga cacatgcgca cgatcttcgg caatctctcg atgcttcagg ccggcggtca
     4441 aacgcatctg gccgagtcgc ttcatcagct cgcagagata ctgacgcgcc gcggcatcgt
     4501 ggtcatcatt tccgacttct acgatcaacc ggatcgtttg caggaatcgt ttcagcatct
     4561 gcgattcaaa ggtcacgacc tggttgcgtt tcaggtgctc gatcaaaacg agctcgactt
     4621 tgatttcacc gatccggtgt tgctgctcga agatgctgaa acgcaggagc agatgcccgt
     4681 gcttcccgac gtggtgatga gtggttaccg tgagcgcatg cgcaaacatg tcgacgagat
     4741 gcggggttgt gcggcggcga ataacgtcga ttttgaaatg ttgacaacaa agcagccgtt
     4801 ggattttgcg ctgttctcgt tcctgtcccg tcgcgcaggc aaataattct tatgtcgttc
     4861 ctgacgccat tatttttcct cggcgtcgcg gcgctcgcgg cgcctattct cgtgcatctc
     4921 gtgcgtcgca cacgtgcgcg tcgcgttcag ttccctgctc tcgtttttgt tcgacaggtt
     4981 ccgcagcgca ccattcgccg tcgcaccttg cataacattc tgctgatgtt attgcgctgt
     5041 ctggcgattc tcctgattgt catagcgttc accagaccat tcttcagcgg cgggagtgcc
     5101 gcgaaagaca acaccgccgc gggcgcgacc gtgatcctcg tcgacaattc tttaagcatg
     5161 cgccgcgagg gtttgttcgc cgaggctcaa cgtcgtgcgg agtcggccgt cgacgacgcg
     5221 cgcaacgacg agcagatcgc tttgatgagt ttcgacaagc gctacacggt gatcaatcgt
     5281 ttcctcacgg ataaaaacag agtgcggacc ggcatcacga gcctggccgc gggctgggac
     5341 gggacggatt acgaacaagc actgcgtgga gcggaatccc tcttgagtga agttgaaacc
     5401 accggtccaa aacggatcgt gatgatctcg gactttcagg cgccgggttg gacgcaggcg
     5461 agcgcaacgt tcaaactgtc gaacagcacg cagctcacta cgctcgacgt cggcggcaac
     5521 aatcctccag ccaacatcgc gatcacgaat gtcgaagcac gcggcgtcgt ttttggacag
     5581 aaatacctcg acaatctcgt ggtccatctc agcaatttca gcgacacgcc gaaagatcac
     5641 atctccgtcg acttccagat caacgatcag actgtcgaga aacgcgacat cagtctcaac
     5701 agtcgcgact cgcgcgtggt tgagttcact ggattcaatc tcaacgacgg cgcaaaccgc
     5761 tgcaccatcg atatcggcac cggagatttc gcgccggaca atcgtttcta tttcactttg
     5821 cgtcgcgaaa cgccggcgaa ggcgctgatt gtcgagggcg cgtcgcgtgg acgaagcgac
     5881 agcctacacc tgcaaagcgc gctcaccgct gacgacgagt tgcctttcgc tttcacgctg
     5941 aagagcgccg gttcgatcga tccctccagt gtttccgagt attcactcgt ggtgctgaac
     6001 gatgccggac cgatttcttc agcactcgcg gatacgctcg tgcggtttgt gaatgcaggc
     6061 ggacagatga tcgtctcgac tggtccaaaa acgcaggctg acactttcaa ccagtcgctg
     6121 caaacgattt tgcccgccgt cttgcgtgaa caggtacaga cgaaagccgg cgagagtgtt
     6181 gcaatcacgg aagtgaaatt cgatcatccg atcttcgaag tgtttcagtc gagtggcagg
     6241 ctcgcatcgg ccaatgtgat cggctacttc cgctccgagc cgcgcgccaa ctcttcggtg
     6301 ctggctcgat tcgaagatgg aagcccagcg ctcgtcgagg catcgacagg caaaggccgt
     6361 gtgcttttgt tcacgtcgtc gttagggccg agctggaacg atctgccgct cacgccgctc
     6421 tacctgccgt tcattcatca aatggtgcgc tacgccggct cgcgtgagga aagcgcgtgg
     6481 agcagtctcg gcgaaacttt tacggtcagc aagcgacagg atccggctcc gcctgcagtc
     6541 gacacgcctg ggggtgtgag actctcggag acgcggctca cacccgacgg agacctgcta
     6601 gttacagcgc gtgagcccgg gttttatcgc ctgcgataca actcggcgcc ggacttcgct
     6661 ggagtaaata ttgacggcgc cgagggtgat ttcacaaagt tgaactttgg tgagtttatg
     6721 gcgggcgtta ccggtggcgc gggtagtgcc gagggcgcta atgcaaatcg taacgccagt
     6781 ggtgaggaag tcgagggacg ccaacgtgtt tggtggttac tgctgcttat cgcgctcttg
     6841 ctattgttgg ccgagtcgtt cctggcgcga cgaacaaaga tggtgaaaat gatcggatag
     6901 atctgcgttt atctgcgttc atccgcggct aaataaggag attgatatga ctcctgaaac
     6961 gaatctttta caggatctcc tgaaacgagc gcgttcgcga cgtcagcttc tgatctcgct
     7021 gcgaggcgtt gcaatcacgg tcggcgttgt cgccgctctt ctgttactga caggctggac
     7081 cgcgcatcgc tttcgctaca acggttgggc gctgatcacc cttcgcgtcg gcgcgttgct
     7141 gatggtcctc gccacgatct actttgccct cttgcgtcca ttgctgaaac gaattagcga
     7201 cgcacgtctc gcacgtctaa tcgaagaaca ctcgcctgga acggaagatc gtctcgtcac
     7261 cgccgtcgaa tactcagacg gcaaccgatc tcacatctcg cccgctatcg tcagccgtct
     7321 ctatcaggat gccaacagtc tttccggaat gctcgacgtc ggaaacgtga ttcgacgttc
     7381 gcgactactg ctttatggcg gcgccgcact cgcgagcctc ctcgtcttcg caggcgtttt
     7441 gaagtttggg ccgaaagaga tctcgcaggg cgtcgcacaa ttggttacgc cgacaacgct
     7501 cgccgcctcg accaacgcga tgagcatcaa ggtgaagccc ggaacggcgc gcgtgcctaa
     7561 aggctccgat caggacatcg tcgcgaccct cgtcaacttc gactcgcaac aagtaacagt
     7621 gttcgcgcgt cccctcggtt caaaaaacga ctttcagggc cagttgatgg aacccgcgaa
     7681 agcaaagact gactttcgct tttccatctt caacattcag gattcgatgg aatacttcgt
     7741 cgagtcgaac aacattcgct ctgaagtctt caaactgaac gtcgtcgatc tccctttcgt
     7801 caaacaactc gatctgacgc tcaacttccc tgccttcacg aatctgccga ccaagaccgt
     7861 cgaagacggc ggcgacatcg cagcacttaa aggcacagtg gccacgatca ctgcgaaact
     7921 gaccggcaaa gttcgcgccg cgcacatcgt gtttcctgac ggcaaaaaga ccgagatgca
     7981 cttgcagggt tcggactttg ttggacagtt aaacgtgacc ggggacacga gctattacat
     8041 cgagctcgtc agcgttgacg gcgaagcgta tcgcggctcg aacgagtacg acgttaccgt
     8101 gctcggcgat cagccgcccg tgatctcgtt tgacaagccc ggtcgcgaca ggaaggccac
     8161 gaatctcgaa gaggtcttca cgcaagcgcg cgccgaagac gactacggcg tggtttcgat
     8221 ggacctgcac ttctccgtca acggcggtga agaaaagact gtcaacctgc aacaactttc
     8281 gcgcgaatcg gcacgcacgc tcaccggcgc ttacacgttc tttctcgagg aatacaatct
     8341 caagcccggc gatttcatct cttacttcgc aaaggcgcgc gatgcgagta atgaagcgac
     8401 cagcgacatc tacttcatcg aagtaaagcc attcgagatg gagtacaagc aatcgcaaca
     8461 gggtggtggc ggtcagggtc agggcggcga tcaggatcag aatgctttgt ctcgtcgtca
     8521 gaaagatctg attgcggcca cgcacaaact ccttcgtgaa ggcgccaagt acacggatca
     8581 ggaacgtaag gacggctacg aagcagtggc tgcgggtcag gagaaacttc gcactgacac
     8641 actcgagttt cttgatcgca tgggccggcg gctcggcgag atcgacgggc aaaagcaagt
     8701 gcaggaaatg gccgagcatc tgaagcaggc cgcgaaagag atggaaggcg cgccgccgcc
     8761 gttgcgaaaa gaggcgggca aagatgcgtt gccacctgaa cagcgtgcgt tgcagcgtct
     8821 gttagctgcc gatgcgatct tccgcgaagt tcaggtcgcg tttggtaatc aaaacagtag
     8881 cggcagtggc ggtggtgagc gcgagcaaca ggagctcgcg ggcttgttcg agctcgagct
     8941 cgacaaaatg aagaaccagt acgagacggt gcagcgtgcg caacagcaac aggccgagca
     9001 acagaagagt gaggccgagc gacgtctcga ggaactcgcc cgccggcaac agcaagcgct
     9061 cgaagagcaa cgtcgacgtc agcaacaaag cggcggaggt ggcggcggca atcagcgaca
     9121 acaacaagag ttga
//
LOCUS       DASSFQ010000294        10618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1630796, whole genome shotgun sequence.
ACCESSION   DASSFQ010000294 DASSFQ010000000
VERSION     DASSFQ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630796"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFH96_10585"
     CDS             <1..568
                     /locus_tag="VFH96_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974462.1"
                     /translation="AHEAYTNAETRSTLGIPLHRLSVCFGAERYSHREYAGKQQLLMV
                     SHDEHPLKERVLAEIDRALPELKIQVIRNISFEDYLEVARTAKWSLTFGEGLDSYFCD
                     PVFNGGVSFAVYNERFFTPAFAKLETVYPSWEVLLNRITTDLRRIDEPGAYTRCWQQA
                     WDILNELYNVERFRENLRAFYRGEYTFP"
     gene            623..1405
                     /locus_tag="VFH96_10590"
     CDS             623..1405
                     /locus_tag="VFH96_10590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6974463.1"
                     /translation="MSLLVNMWRSIPEGIRFPILRVVQRNKPVYRWLMFRHADMKDRA
                     NGKWDHLPPAELRYRVGGSPDAAEFVMIGKTCADDIQAALQQVGRELSSFDRILDFGC
                     GCGRTLVHMKALAPDSQFDGIDIDANAIAWCKNNLDFANFRLSKETPPIDYPANTFDC
                     IYVISVFTHLDEDYQFRWLEELQRISKPGAVLVLTVNGSKNGQGFFFERSYEEGLFPA
                     WYQNTYHSKDYVFENFGKYFEVLKHLPRNMNAHQDVVVLQKR"
     gene            1490..2329
                     /locus_tag="VFH96_10595"
     CDS             1490..2329
                     /locus_tag="VFH96_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974464.1"
                     /translation="MESVRLPAEPLVVIQPTSRWTLVSFKDIWAYRELLFFLTWRDVK
                     VRYKQTALGAAWAILQPLFMMIIFTIFFGRIAGVASAGIPYPVFALAGLVPWTFFSNS
                     ITASGNSLVGSANLITKVYFPRLIVPAAAMLAGLVDFVLAFVLLGGLMVYYQVVPTMQ
                     IVFLPVLVLLTALFSLGVGTWFAALNVKYRDVRFALPFLIQLWLFVSSVILPSTAIPP
                     KWRWLLTLNPMSGIIEGYRSALFGLPFDWPALGIASGLTILTLLYAIYTFGRTERSFA
                     DII"
     gene            2366..3613
                     /locus_tag="VFH96_10600"
     CDS             2366..3613
                     /locus_tag="VFH96_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974465.1"
                     /translation="MQPIIRVQGVGKQYHLGGPTAVYSTLRETIMDLARNSVRALRKN
                     GDNNILWALQDLDFEVSPGEVLGIIGRNGAGKSTLLKILSRITEPTVGRVELFGRVAS
                     LLEVGTGFHPELSGRENIFLNGSILGMTRKEIERKFDEIVAFSEVEKFLDTPVKRYSS
                     GMYVRLAFAVAAHLQPEILIVDEVLAVGDYAFQQKCLNKMQDVSTGGRTVLFVSHNLG
                     AVSRLCNRCLLLDKGRLLDAGPTADVVQSYMTSGLIQRPEFVQPSNREKAMNLRRISV
                     KGSDGEVRSEIRYDESLVFEFVYETNQTISGVSLGFMIFTIDGTCAFTSADIDAHSEL
                     LAGREPGTYQTQVTIPAKWLNVGRYMVKVSLANAMSGVAYDWVTDLGFQIIDTGTPGS
                     LNGIKRPGILQPVLDWETRMIAD"
     gene            3654..4649
                     /locus_tag="VFH96_10605"
     CDS             3654..4649
                     /locus_tag="VFH96_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6974466.1"
                     /translation="MKLFLPNNLSRSRAEFTPHNDPEIASMLEQHYGLSEPQISVFAG
                     ANVSARNFKIETRKAKFFLKSRELDAIEKTQHEALITFELGVMRQKVPRIIWPKGKEK
                     ELVTVFADRCWMLYRFQEGDYFTGKGEQLSSAAKAFGELSKQARLRISSTKKKLDVLP
                     VGLPKLLERSTIPADQRAVILKNLTRVEQHRELLNDHWLPVHLDFHPLNLLMNDDSVA
                     CIVDLEHLKPYPVVIGLGFAGFKLIRQAMVDDEYRARESHAVQTWLRGWQESFPDAQF
                     TVEELGLGARARILTLIHMILDASLNHNDDRSSYDLEKQIVSLYEADVIFGTDRL"
     gene            4668..5456
                     /locus_tag="VFH96_10610"
     CDS             4668..5456
                     /locus_tag="VFH96_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6974467.1"
                     /translation="MMIIDLTGKTALVTASSKGIGFGISKALLAAGANVCICSRNEDN
                     LRTAESELAQPDRVLAITGDVADAAFLKELVSRAVDRFKVIDILVNNSGGPPAGEALS
                     LTEEQWLGAINGNLLSVIRLSSLVIPQMKNKGWGRIVNLTSTAAREPGAGMALSNVTR
                     AGVAAYTKTLAQEVGPFGITVNTILTGGVLTDRLNSLLERSIKDTGETFDEAIARIEK
                     TIPVRHLSTPEEFAQTTLFLVSPQASYINGAAIAVDGGASKSTF"
     gene            5456..6172
                     /locus_tag="VFH96_10615"
     CDS             5456..6172
                     /locus_tag="VFH96_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974468.1"
                     /translation="MNTATTSINFCLQPVAVEAAEKVSSMTQRPEESEKALLKLCAER
                     LSETQLKTVQRSLAYAKSLESTDPNHPSMRAYFSHPLRVARLSLQLLDQPSPEIVSMG
                     LLHNVFEVSGLSEANMLQAGFSERFAIGIRLNTIDRERQYDEAYLTQFYGRMQEFGDD
                     LTLIKCVDKLDNLLAFELFERTPQIERYMALCEQFVTPMAAALSPAFGDYVQDTIAYM
                     RSQGCNETLRAQYQDFVGKA"
     gene            6173..7252
                     /gene="neuB"
                     /locus_tag="VFH96_10620"
     CDS             6173..7252
                     /gene="neuB"
                     /locus_tag="VFH96_10620"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HET6974469.1"
                     /translation="MNDALTGAQQPLNVRIGDRLVGPGQPVFVIAEAGVNHNGDLKLA
                     RALIDVAVDAGADAVKFQTFHADLLAVSDAPKAAYQLETTDTQESQLEMLRGLELSAD
                     AHRELRSYCDERGIVFLSTPFDEQAVDMLDELGVPAFKISSGDLTNSPLLEYAAGKKK
                     PMILSTGMSELAEVIEAVSVLHAAGCENPILLHCVTNYPAEPPEVNLRAMQTMRSAFN
                     VPVGFSDHTEGIDVPLAAVALGACVIEKHFTLDRSLPGPDHRASLEPDELRQLVQSIR
                     RVEVALGNGRKVPQPSEIETAKVARRSLVAATNIPAGATIEREMIVLRRPGTGLPPAE
                     ITTLLGRRARREIAAGSLLAADMFE"
     gene            7305..8282
                     /locus_tag="VFH96_10625"
     CDS             7305..8282
                     /locus_tag="VFH96_10625"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6974470.1"
                     /translation="MVFRGEITTETQRTQRLHRESLRTIEDAIRLNVWITGCSGFLGT
                     RLAMHLVAEHIPVTSLSRRKSSAATRSVEIDLAQRDAPRQIEKLATETAPDIVIHAAS
                     KQPTGGELADFVDANVHSSSNLIEGLTQKPPRLIIYTSTHSVYGKQSILPVDESCPAG
                     GYLPYGATKRWGEQMLETFQRYSKIVVLRLPSLYGAGQEDSFIDGLARLAQRDEPIEL
                     FSRGLLIRDALHVSDVVKAIDNCIARPPSAAFSIMNLGCGQSIKTIEWANALLSVLPS
                     DSEIVPVDREAKYFDLYADITVAQERIGFAPTSLADSMKVYADELSTRS"
     gene            8254..9432
                     /locus_tag="VFH96_10630"
     CDS             8254..9432
                     /locus_tag="VFH96_10630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6974471.1"
                     /translation="MPTNYQHDPDHPLTTAFPYFPPEEQEWLSGELSQILNGMLAMGP
                     RVAKFEQEFAAYCGRSHGIAFPSCTSSMEAALLAFGVGPGDEVLVPVETFIATGMVVS
                     LVGARPVFTEISAANFSMDFEDAWSRVTEKTRGAIVVHYGGFISPQLPAFAQKMRDAG
                     LFVIEDAAHAPGAELNGQKAGSLGDAGCFSFYPTKIMTTGEGGMLVTNRDDIARTVRS
                     LQLRGKDLDHKEERYVTPGRNNRFTEIAAAMGLSQLRSLPDFLASRRRVAAVYDEKLL
                     ASELFVPLLAEDGSVPAYWRYVAIPTVDIDRAELRDRLAADKITIDWAYDPPLHLQPV
                     FQTTFGTKPGMLPRSENIMAKHICLPVHARMRDTDAEFVAERLLFHVRSLAQPAPQTR
                     "
     gene            9452..10156
                     /locus_tag="VFH96_10635"
     CDS             9452..10156
                     /locus_tag="VFH96_10635"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HET6974472.1"
                     /translation="MKIAIHQPQYFPWPRYVHKAIAADIFVYLDTVQFSKNGLQNRNQ
                     IKTVNGASWLTVPVKHEFGNSIRETQIADKRATTKHWKTLQANYSQAVGFQCWSDELR
                     KLLDGEITSLAELAIASTEWMLEKLNVQTKRVRASELAGIDGNASKLVASICKTLDAD
                     TYLTGTGALDYLFKGDFDAIDCEIRVQQWQPFSYRQQWEKIGFVADLSTLDLLLNCPD
                     EASEMLNTAGSWKALA"
     gene            10153..>10618
                     /locus_tag="VFH96_10640"
     CDS             10153..>10618
                     /locus_tag="VFH96_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HET6974473.1"
                     /translation="MKKRQVLAITAIRSEYYLQRSILRAIMDHPELELSLIVTGAHLS
                     PLHDYTVKEVEADGFPIVERIENLMYSDRDAARLKGAAIQFQALAQVADTRRPDWLFL
                     PGDREETITLGLCGAYLGLAMAHYAAGDRVVGNVDDMIRHSVSRLSHLLLTTN"
BASE COUNT         2320 a         2838 c         3013 g         2447 t
ORIGIN      
        1 cgcgcatgag gcttacacaa atgctgagac tcgcagtacg ctcgggattc cgcttcatcg
       61 actttcggtt tgttttggcg ctgagcggta tagtcatcgc gagtatgccg gcaagcagca
      121 gctcttgatg gtctcgcatg acgagcatcc gctcaaagaa cgagtgctgg ctgagatcga
      181 ccgtgcgttg cctgagttga agattcaggt gattcggaat atttcgtttg aggattatct
      241 ggaagtagcg cggacggcga agtggtcgct gacgtttggt gaagggttgg atagttattt
      301 ctgcgatccg gtttttaatg gtggggtgtc gttcgcggtc tataacgagc gtttctttac
      361 accggcgttc gcgaaactcg aaaccgtgta cccatcgtgg gaagtgctcc ttaacaggat
      421 cacgaccgat ctccgccgca tcgacgagcc cggcgcctac acacgttgtt ggcagcaggc
      481 gtgggatata ctgaacgagc tttacaacgt cgaacgattc cgcgagaact tgcgagcgtt
      541 ctatcgcggt gaatacacgt tcccgtgaat atatattagc cgcggattta cgcggatgaa
      601 cgcggatcag taggagatca agttgagttt gttggttaac atgtggcgat cgataccgga
      661 ggggattcgc ttcccgattt tgcgggtggt gcagaggaat aagcctgtct atcgctggct
      721 gatgtttcgc catgcggaca tgaaagatcg cgcgaatgga aaatgggatc acctgccccc
      781 ggctgaatta cgttatcgcg tgggcggctc tccggacgcc gcagagtttg tcatgatcgg
      841 caagacgtgc gcagacgaca tccaggcagc actccaacag gtcggccgtg agttgagttc
      901 gttcgatcgc attctcgact tcggctgcgg ttgtggtcgc acactcgtgc acatgaaggc
      961 gctcgccccc gattcacagt tcgacggcat tgacatcgac gccaacgcga tcgcctggtg
     1021 caaaaacaat ctcgacttcg caaacttccg tctgagcaaa gaaacgccgc cgatcgacta
     1081 ccctgcaaac acgtttgact gcatttacgt gatctcggta tttacacatc tcgacgaaga
     1141 ctatcagttt cgctggctcg aagagttgca acgcatttcc aaaccaggcg ccgtgctggt
     1201 tctgactgtg aacggctcga agaacggtca gggtttcttt tttgaacggt cgtacgagga
     1261 gggactgttc cctgcctggt atcagaatac atatcactca aaagattacg ttttcgaaaa
     1321 cttcggcaag tacttcgagg tgctgaaaca cttgccccgc aacatgaatg cccatcagga
     1381 cgtggtagtg ctgcagaagc gctagtcaga gttctttatc cacagattac gcagattttt
     1441 taaaggcatc tgtgtaatct gtggatagat tctatatact gtcgccccta tggaaagtgt
     1501 tcgccttccg gccgagcctc tcgtcgttat tcaaccgact agccgctgga ccctggtaag
     1561 cttcaaagac atctgggcct atcgtgagtt gctcttcttt ctcacgtggc gcgacgtcaa
     1621 agtgcgttat aagcagacgg cgctgggcgc tgcgtgggcc atcctccagc cgctcttcat
     1681 gatgatcatc tttacgattt tcttcggcag gatcgccgga gtagcttcag caggaattcc
     1741 gtatccggtc ttcgcgctcg ctggactcgt tccgtggacg ttcttttcca actcgatcac
     1801 agcgagcggc aacagtctcg tcggcagcgc caacctgatc acaaaagttt acttcccacg
     1861 tttgatcgtg cctgcggcgg caatgctggc cggcctggtt gatttcgtgc tggcgttcgt
     1921 gctgctcggc ggtttgatgg tctattacca ggttgtcccc acgatgcaga tcgtgttcct
     1981 gccggtcctc gttttattga cggcgttgtt tagtctcggc gtgggcacgt ggttcgccgc
     2041 gctgaacgtc aagtatcgag acgttcgctt tgcgctgccg tttctgatcc agctatggct
     2101 gtttgtctcg tcagtcattc ttccttcgac tgcgattcca ccaaagtggc gctggttact
     2161 gacgttgaat ccgatgtcgg gaattatcga aggctatcgc tccgctcttt tcggactgcc
     2221 gtttgactgg cctgcgctcg gcatcgcttc aggtttgacc atcctgaccc tgctctatgc
     2281 gatctatact tttggacgca cggaacgcag cttcgcagat atcatctgac cgctatcagt
     2341 ctgaccgccg ccgcggaaca tctgcatgca gccaatcatc agagtgcagg gagttggaaa
     2401 acagtaccac cttggtggac caaccgccgt ttactcaacc ctgcgcgaaa cgatcatgga
     2461 cctcgcgcgc aactccgtgc gcgcgctgcg caagaacggc gacaacaaca tcctctgggc
     2521 gttgcaagat ctcgacttcg aggtctcccc gggtgaagtg ctcggcatca tcggtcgcaa
     2581 cggcgccggc aaatcgacgt tactgaaaat actttcacgg atcaccgagc cgactgtcgg
     2641 tcgggttgaa ttgtttggcc gcgtggcgag tctgctggaa gtgggaaccg gatttcatcc
     2701 ggagctgagc ggacgtgaaa acatctttct caatggctcg atcctcggca tgacgcgcaa
     2761 agagatcgag cggaagtttg acgagatcgt cgcattttca gaagtcgaga agtttctcga
     2821 cacgccagtg aagagataca gcagcggtat gtacgtgcgg ctcgcgtttg ccgtggcggc
     2881 gcatttgcaa ccggagattc tgatcgtcga cgaagtgctg gcggtaggtg attatgcgtt
     2941 tcaacaaaag tgcctgaaca agatgcagga tgtttcgacc ggcgggcgaa cagtgttgtt
     3001 tgtcagtcac aatctgggcg cagtcagtcg tttgtgtaat cgttgtctac tgctcgataa
     3061 aggccgtttg cttgatgccg gaccgaccgc ggatgtggtt cagagctaca tgacgagcgg
     3121 gctgatacag cgaccggagt ttgtacagcc aagcaatcgc gagaaagcga tgaaccttcg
     3181 gcggatctcg gtgaaaggtt ctgacggtga ggttagaagc gagattcgtt acgacgagag
     3241 tttggtgttt gagtttgttt acgaaacgaa ccagaccatt tctggtgtga gtctcgggtt
     3301 catgatcttc accatcgacg ggacgtgtgc tttcacttcc gctgacatcg acgcgcactc
     3361 ggagttgctc gccgggcgtg aacccggaac atatcaaacg caggtcacga ttccagccaa
     3421 atggttgaat gtcggtcgct acatggtgaa ggtgtcgcta gcgaatgcga tgagcggagt
     3481 tgcgtacgac tgggtcacgg atcttgggtt tcagattatc gacaccggaa cgccggggtc
     3541 gctgaacggg atcaagcgac cgggcatact gcagccagtg ctcgactggg aaacgcggat
     3601 gatcgcggat tgaaatccgg atctatccgt gtttgatccg tggctaaatt cttatgaagt
     3661 tgttcctgcc gaacaatctt tccagatctc gggccgagtt cacgcctcac aacgatccgg
     3721 aaatcgcttc aatgcttgag caacactacg gtttgtcgga gccgcagatc tcggtttttg
     3781 cgggcgcgaa tgtctcggcg cgcaatttca agatcgagac caggaaggcg aaattttttc
     3841 tcaagtcgcg tgaattggat gcgatcgaga agacgcaaca tgaagcattg atcaccttcg
     3901 agcttggagt catgagacag aaggtgccgc gcattatttg gccgaagggc aaggagaagg
     3961 agcttgtcac tgtctttgcg gatcgatgct ggatgcttta caggtttcag gagggcgatt
     4021 acttcacagg aaaaggcgag cagctgtcgt cggctgcgaa agctttcggc gagctttcga
     4081 aacaggcgag gttgcgaatc tccagcacca agaagaaact cgatgtgctt ccggttggtt
     4141 tgcccaagct gcttgagcgt tcgacgattc cagccgatca gcgcgccgta attctgaaaa
     4201 acctcacgcg cgtcgagcag catcgcgagt tgctcaacga tcactggctg ccggtgcacc
     4261 tcgatttcca tccgctcaac ttgctaatga atgacgacag cgttgcatgt atcgtcgatc
     4321 tggaacactt aaaaccgtat ccagttgtca ttggacttgg cttcgctgga tttaagttaa
     4381 tccgtcaggc gatggttgac gatgaatatc gggcgcgcga atcacatgcc gttcagacgt
     4441 ggctgcgagg ctggcaggag agtttccctg acgctcaatt cactgtcgaa gagcttggtc
     4501 tcggcgcgcg tgcgcgcatt ctcaccttga tccacatgat cctcgacgct tctctaaatc
     4561 ataacgacga tcgctccagc tacgacctcg aaaagcaaat cgtcagtctc tacgaggctg
     4621 atgtgatctt tggtacggat cgtttatagt acttttcact aaacaaaatg atgattatcg
     4681 atctgaccgg caaaacggcg ctcgtcaccg ccagcagtaa aggcatcggt ttcggcatca
     4741 gcaaggcgct actcgctgcc ggcgccaatg tctgtatttg ctcgcgcaac gaggacaacc
     4801 tgcgcacggc cgaaagcgaa ctggcgcaac cggatcgcgt gctcgcgatc acaggcgacg
     4861 tcgccgatgc tgcctttctc aaagagctcg tcagccgtgc cgtcgatcgc ttcaaagtga
     4921 tcgacatact cgtcaacaac agcggtggtc caccagcagg cgaagcgctt tcattgacag
     4981 aggaacagtg gctcggcgcg atcaacggaa acttgttgag cgtgatcaga ctctcgagcc
     5041 tcgttattcc gcaaatgaag aataaaggtt ggggccggat cgttaacctg acttccaccg
     5101 ccgcgcgcga gcccggcgcc ggcatggcgc tctcaaacgt cacgcgcgca ggcgtggccg
     5161 cgtacacaaa aactcttgca caggaagtgg gaccgtttgg catcaccgtc aacacgatcc
     5221 tcaccggcgg cgtgttgact gatcgactca attcacttct cgaacgaagt atcaaagata
     5281 ccggcgagac gtttgacgaa gccatcgcgc gcatcgaaaa aacgattccc gtccgacacc
     5341 tttccacgcc cgaagagttt gcgcaaacca cgctctttct cgtttcgccg caggcgagtt
     5401 acatcaacgg cgctgcgatt gccgtggatg gtggagcgtc gaagagtact ttctgatgaa
     5461 caccgcaacc acgtcgatca acttctgctt gcaaccggtt gcagtcgagg ctgccgagaa
     5521 agtttcgagc atgacgcagc gaccggaaga gtcggaaaag gcgttgctga agttgtgcgc
     5581 cgagcggttg tctgagactc agttgaaaac ggtgcagcga tcgctggcgt atgccaagtc
     5641 gctcgaatca actgatccaa accatccgtc gatgcgcgcg tacttcagcc atccgctgcg
     5701 tgtcgcccgt ctctcgttgc agttactcga ccaaccgtcg ccggaaatcg tttcgatggg
     5761 actgctgcac aacgttttcg aggtcagcgg gctgtccgaa gcaaacatgc tccaggccgg
     5821 gtttagtgag cgtttcgcga ttggaatccg cttgaacacg atcgatcgag aacggcaata
     5881 cgacgaggca tacctcacgc agttttacgg gcgcatgcag gagtttggcg acgacctgac
     5941 gctaatcaaa tgcgtcgaca agctcgacaa cctgctcgcg tttgagctct tcgagcgcac
     6001 gccgcaaatc gagcgctaca tggctctctg cgaacaattc gtcactccga tggccgcggc
     6061 attgtcgccc gcgtttggag attacgttca agacacgatc gcttacatgc gttcgcaggg
     6121 ctgcaatgaa actctgcgag ctcagtatca ggatttcgtg gggaaggctt aattgaacga
     6181 cgctttgact ggcgcacagc aaccactaaa cgtgcggatc ggtgatcggc tcgtcggacc
     6241 gggtcagcct gtttttgtga tcgcggaggc tggcgttaat cacaacggcg atctgaagct
     6301 tgcccgcgcg ctcatcgacg tcgctgttga tgccggcgcc gatgccgtca agtttcagac
     6361 ttttcatgcc gatctgctcg ccgtctccga cgcgccaaaa gctgcgtatc aactcgaaac
     6421 tactgacacg caggagtcgc agttagagat gctgcgcggg ctggagttat cggctgacgc
     6481 gcaccgggaa ttgcgttcat actgcgacga gcgcggcatc gtttttcttt cgacgccgtt
     6541 tgacgaacaa gcagtcgaca tgctcgacga gctgggcgtt ccggcgttca agatcagttc
     6601 cggcgatctg acgaattcgc cgctgttgga atacgcagcc ggtaagaaga agccgatgat
     6661 tctttcaacc ggcatgtcgg agcttgcaga ggtgatcgaa gctgtcagcg tgttgcacgc
     6721 agcgggctgt gaaaatccga tactgctgca ttgcgttacg aattatccgg ccgagccccc
     6781 tgaagtaaat ctgcgagcga tgcaaacgat gcgttcggcg ttcaatgtgc ccgtcggctt
     6841 ttcggatcac acggaaggaa tcgatgttcc tctcgcggcg gtggctttag gagcatgcgt
     6901 gatcgagaag cacttcacgt tggatcgttc actgcctggt cccgatcatc gtgcttcact
     6961 cgagcctgac gagttgcgac agctcgtgca atcgatccga cgtgttgaag tggcgctcgg
     7021 caacggacgc aaggttccgc aaccgagcga gatcgagaca gcaaaagtcg cgcgtcgcag
     7081 tctcgttgcg gccacgaata ttccggcagg cgcgactatc gaacgggaga tgatagtgct
     7141 gcgtcgcccc ggaaccggtt tgccaccggc agaaatcacg acgttgctgg gccgtcgcgc
     7201 acgtcgcgaa attgcagcgg gcagcctgct cgccgccgac atgttcgaat aagaatggag
     7261 ttcttctctg tgcaacctct gtgttctctg tgtctctgtg gttgatggtt ttccgagggg
     7321 agatcaccac agagacacag agaacacaga ggttgcacag agaaagccta agaacgatag
     7381 aggacgcgat tcgcttgaat gtttggataa cgggttgcag cggattcctg ggcacgcgat
     7441 tggccatgca tctggtcgcg gaacacatcc ctgttaccag tctctcgcgg cgcaagagct
     7501 cggcggcaac gcggtcagtc gaaatcgatc ttgcacaacg cgatgcgcct cgacagatcg
     7561 aaaagctggc aacggaaacc gctccggaca tcgtcattca cgccgcgtcg aagcaaccga
     7621 ctggaggcga gttagccgac ttcgtcgacg caaacgtcca ttcctcttca aacctgatcg
     7681 aaggactaac gcaaaagccg ccgcgcttga tcatctacac ttcgacacac agcgtttacg
     7741 gaaagcaatc gatactcccg gtcgacgaat cgtgtcccgc cggaggatac ttgccttacg
     7801 gcgccaccaa gcgctggggc gaacaaatgc tcgaaacatt tcagcgttac tctaagatcg
     7861 tagtgctgcg gttgccgagt ttgtatggcg cgggtcagga agacagtttt atcgacggtc
     7921 tcgcgcgcct ggcgcaacgt gacgagccga tcgaattgtt ttcgcgcggg ctattgattc
     7981 gcgacgcatt gcatgtgagc gatgtagtaa aggcgatcga caactgtatc gcacgtccgc
     8041 cgagtgctgc tttttcgata atgaaccttg gctgcggcca gtcgatcaaa acgattgaat
     8101 gggcgaatgc gcttctctcc gtgttgccga gcgatagcga gatcgttcct gtcgatcgcg
     8161 aagcaaagta tttcgatctt tatgctgata tcacggtggc gcaggaacgg atcggattcg
     8221 caccgacgtc gctcgccgac tcgatgaaag tttatgccga cgaattatca acacgatcct
     8281 gatcacccgc tcacgaccgc ctttccctac tttccaccgg aagaacagga atggctctcg
     8341 ggcgaattga gccaaatact caacggcatg ctcgcgatgg gcccgcgcgt ggcaaagttt
     8401 gagcaggagt ttgcggctta ttgcggacgc tcgcacggca tagcgtttcc gagttgtact
     8461 tcgtcgatgg aagcagcgtt gctcgcgttc ggagtcgggc caggtgatga agtgctagtg
     8521 ccggttgaga cattcatcgc cacgggcatg gttgtttcac tcgttggcgc tcgtcctgtt
     8581 tttacagaaa tctccgccgc taacttctcg atggattttg aggacgcgtg gtcgcgtgtg
     8641 acggagaaaa ctcgtggcgc aatcgtggtc cactacggcg gatttatctc gccgcaactg
     8701 cccgcgtttg cacaaaaaat gcgcgacgcc ggtctctttg taattgaaga cgcggcacac
     8761 gcacccggcg ccgagttgaa tggccaaaag gctgggagcc tcggcgatgc aggctgcttc
     8821 tctttttatc cgacaaagat catgaccacc ggcgagggcg gcatgctggt cacgaatcgc
     8881 gacgacattg cgcgcactgt acggagtctt cagttgcgtg gcaaagatct cgatcacaaa
     8941 gaagaacgtt acgtgacgcc aggtcgcaat aatcgattta ctgagatcgc cgcggcgatg
     9001 ggtctgagtc aactgcgctc gctgcctgac tttctggcaa gtcgccggcg cgtggcggca
     9061 gtctatgacg agaagttgtt ggcgagtgaa ctcttcgtgc cgttgctggc agaagatggc
     9121 agcgtgccgg cctactggcg ctacgtcgcg attccgactg tcgatatcga tcgggctgaa
     9181 ttaagagatc gactggccgc cgacaagatc acgatcgatt gggcatacga cccaccgcta
     9241 catctgcagc cggtgtttca aacaaccttc ggcacaaagc ccggcatgtt gccgcgctcg
     9301 gaaaacatca tggcaaagca tatctgcctg ccagtgcacg cccggatgcg cgatacggac
     9361 gccgaatttg tcgccgagcg actgctgttt cacgtccggt cgctggctca gccggcgcct
     9421 caaactcggt gaagccctct gtgaagcatt tgtgaagatc gcgatccacc aaccgcaata
     9481 tttcccctgg ccgcgttatg tgcacaaagc aatagctgct gatatattcg tctaccttga
     9541 tacggttcaa ttcagcaaga acggtttaca gaaccgcaat caaatcaaaa cggtaaatgg
     9601 cgcatcgtgg ctgaccgttc cggtcaaaca cgagtttggc aactcgatcc gcgaaacgca
     9661 gatcgcagac aagcgagcga cgacgaagca ttggaagacg ttgcaggcaa actattcgca
     9721 ggctgtggga ttccagtgct ggagtgatga acttcgcaag ttattggacg gtgaaatcac
     9781 ctcgctcgct gaactcgcaa ttgcttcgac agagtggatg ctcgaaaagt tgaatgtgca
     9841 gacaaaacgg gtgcgagctt cagagcttgc gggcatcgac ggcaacgcct ccaaattggt
     9901 cgcttctatt tgcaaaactc tcgacgcaga cacgtatctc acgggcactg gtgcgctcga
     9961 ttacctcttc aaaggtgatt ttgacgccat cgactgcgag attcgcgtcc agcagtggca
    10021 gccattcagc tatcgccaac agtgggagaa gatcggcttc gtggccgatc tctcgaccct
    10081 tgatctcctt ttgaactgtc ccgatgaagc ttctgaaatg ctgaacaccg cgggtagttg
    10141 gaaagctctg gcatgaagaa acggcaggtc ctggcaatca cggcaattcg ctcggagtat
    10201 tatctacagc gctcgatact ccgcgcgatc atggaccatc cggaactaga gctgtcgctg
    10261 atcgtcactg gcgcgcacct gtctccgctg catgactaca cggtcaaaga agtcgaggct
    10321 gatggttttc cgatcgtcga acgaattgag aatctgatgt attcggatcg cgatgcggca
    10381 cgactgaaag gcgccgcgat tcagtttcag gcgctcgcgc aggtggctga cacgcgccgg
    10441 ccggattggc tgttccttcc cggcgatcgt gaggagacga ttaccttagg tttgtgtggc
    10501 gcatatctcg gtctggctat ggctcactac gccgccggcg atcgcgttgt tgggaacgtt
    10561 gacgacatga ttcggcattc cgtgtcacga ctctcgcatc ttttattgac gactaacg
//
LOCUS       DASSFQ010000295         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1328704, whole genome shotgun sequence.
ACCESSION   DASSFQ010000295 DASSFQ010000000
VERSION     DASSFQ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328704"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFH96_10645"
     CDS             <1..268
                     /locus_tag="VFH96_10645"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974474.1"
                     /translation="KTAERLILELREKVADLSSAQLEEELGAKPEAGEPSEDTVRADA
                     LLALLNLGYQRSAAEKAIDAALGDGGDVTVETILRRSLKKLARV"
     gene            276..1319
                     /gene="ruvB"
                     /locus_tag="VFH96_10650"
     CDS             276..1319
                     /gene="ruvB"
                     /locus_tag="VFH96_10650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET6974475.1"
                     /translation="MPPESISARVKDSLPSDDDRQFDLTLRPTRIDEYIGQRQVKENL
                     RVYMKAARNRREALDHVLLTGPPGLGKTTLANIIANEMGAELRSTAGPAIEKAGDIAA
                     LLTNLQEGDVLFIDEIHRLLPALEEKLYPAMEDYQLDLLIGQGAAARSIKLELPKFTL
                     IGATTRAGLITAPLRGRFGIVFHLDFYPHDDLEVICKRSAEILGVEIDNDGAREIARR
                     SRGTPRIVNRLLRRVRDYAQVDYDGRINQEVANDALNRMEVDTYGLDEMDRKLLLTII
                     EKFGGGPVGLGTISASIHEEKDSIEEIIEPYLIQIGFLDRTPRGRLATPLAYEHFGIK
                     TPGSRIPSLFDPR"
     gene            1388..2287
                     /locus_tag="VFH96_10655"
     CDS             1388..2287
                     /locus_tag="VFH96_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6974476.1"
                     /translation="MAKFPMKRWPSFSRHSFGVRNRSPWRSMLGHSLHQFHENDLFTS
                     AAAMSYFGLMALFPALLFMFAISNKLAAGSQLLTHAVDVYPGSSKFLRDTIEAFSDIG
                     VGAVITCIILIFWAGSWVFAVIERALNRIWGATSRTFLHGRALTIGMVGLVGLLLASS
                     VLVTSILVALREMADRLSPRQIERYTLLASVGSAFWQVLFATLSYLVTVALFVLVYRF
                     MPKAEVTLRDTLPGAFLAGLLWEIAKYIFALSLHYFHYDQVYGSVGAVVAVLTWSYVS
                     SLILLFGAQLTAVFHYEHPATTD"
     gene            2293..3315
                     /gene="queA"
                     /locus_tag="VFH96_10660"
     CDS             2293..3315
                     /gene="queA"
                     /locus_tag="VFH96_10660"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET6974477.1"
                     /translation="MKIADFDYELPADLIAQTPLEERDTSRMLVLDRRDQAWSDSGFK
                     EFTRYLRPNDVVVVNNSRVIPARLLGRREESGGQVEIFLVREIEPEIWEALVRPGGRL
                     KKGARAVFGEGRLIAELQDEPGSEMRRVRFHCDGPFHDVLAQIGSTPLPPYIKRPAGV
                     SNEDRERYQTVYSKQRGAIAAPTAGLHFTPEVLEEVAKIAGLAEVTLHVGYGTFEPVR
                     VDDVNQHSVGSEHYEISEVAAQTINEAERVIVVGTTTMRALESSANERGEVHAGPGVA
                     SLTIKPGYRFRVADALLTNFHLPRSSLLILVSAFAGRDLILRAYGHAVAERYRFYSYG
                     DCMLIL"
     gene            3355..3906
                     /locus_tag="VFH96_10665"
     CDS             3355..3906
                     /locus_tag="VFH96_10665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET6974478.1"
                     /translation="MAVLKKFRSRWWSPSDVEFDSEKKEKTPTPSWGTSYDLRPLTIS
                     QLDEVWRLDQRCFIDGEAYSRETFDYLLTAPESVSYRAVTPGGVMAGFVIGLVEPDHT
                     GHVTTVGVAPEHRRRSLAKRLMGQVEDGFRKRNVRLVRLEVRTMNIAAQKLYESLGYS
                     ITQKLPKYYSNGGDGLLMLKSLE"
BASE COUNT          868 a         1133 c         1163 g          887 t
ORIGIN      
        1 caaaaccgct gagcgcctga tcctggagct gcgtgagaaa gtcgcggacc tatcgtctgc
       61 tcaactcgag gaagagctgg gcgcgaaacc cgaagctggc gagccttcgg aagacacggt
      121 gcgcgccgac gcgcttttgg ccctgctcaa tcttggttac caacgctcgg ccgcggaaaa
      181 agcgatcgac gccgcactcg gcgatggcgg cgacgtcacg gtcgaaacga tcctgcgacg
      241 cagtctgaag aaactcgcaa gggtttgaaa tcaacatgcc tcctgagtca atatcagcgc
      301 gcgtgaaaga cagtcttccc agcgacgacg atcgccaatt cgatctcact ctgcgcccga
      361 ctcgtatcga cgaatacatc ggccagcgtc aggtcaagga gaacctgcgc gtctacatga
      421 aagccgcgcg caatcgtcgc gaagcgctgg accacgtgct gctcaccggc ccgccgggtt
      481 taggcaaaac cacactcgca aacatcatcg ctaacgagat gggcgccgag ttgcgctcga
      541 ccgccggtcc cgcgatcgag aaagcaggcg acattgccgc actgctgacc aaccttcagg
      601 aaggcgatgt cctctttatc gacgagattc atcgcctgct gcccgcgctg gaagaaaaac
      661 tctacccggc gatggaggac taccagctcg atttgctgat cggtcagggc gctgcggcac
      721 gttcgatcaa actcgagctg ccgaagttca cgcttatcgg cgctacgaca cgtgccggtc
      781 tgatcacggc gccgctgcgt ggtcgtttcg gtatcgtctt tcatctcgac ttttacccgc
      841 acgacgatct cgaagtgatc tgcaagcgct cggctgagat cctcggcgtc gagatcgaca
      901 acgacggcgc gcgtgagatc gcacgacgct cgcgcggcac gccccgcatc gtcaatcgac
      961 tgctgcgtcg tgtgcgtgac tatgctcaag tcgactacga cggccgcatc aaccaggaag
     1021 tcgcaaacga tgcgttgaat cggatggaag tcgacacgta cgggctcgac gaaatggatc
     1081 gcaaactgct gttgacgatc atcgagaagt ttggtggtgg accagtcgga ctgggcacga
     1141 tttccgcgtc cattcacgag gagaaagatt caatcgagga gatcatcgaa ccgtatttga
     1201 tacaaatcgg attcctggat cggacgccgc gcgggcgact ggcgacaccg ctcgcgtacg
     1261 aacacttcgg tattaagacc cccggctcaa gaatacccag cctgttcgac ccccgctgaa
     1321 gatgattttg agccaccgat gaacacagat gaacacggca aagaaactgt gttaatccgt
     1381 gaaatccgtg gctaaatttc cgatgaaacg ctggccttca ttttcacgtc actcattcgg
     1441 cgttcgaaac cgctcgccgt ggcgctcgat gctcggccac tcgctgcacc agtttcacga
     1501 aaacgatctc ttcacgtctg cggcggcgat gagttacttc ggactcatgg cgctcttccc
     1561 ggcgctgttg ttcatgttcg caattagcaa caaactagcg gcgggcagtc aactcctgac
     1621 ccacgcagtc gatgtttatc ccggctcgag caagtttctg cgtgacacga ttgaagcgtt
     1681 ttcagatatc ggagtgggtg cggttatcac gtgcatcatt ctcatcttct gggccggctc
     1741 gtgggtgttt gcagtgatcg aacgtgcact caatcgcatc tggggggcga cgtcgagaac
     1801 attcctgcat ggacgcgcac tgacgatcgg aatggttggg ctggttggat tgctactggc
     1861 gtcttcggtt ttggtgacgt cgattctcgt tgcgcttcgt gaaatggccg accggctctc
     1921 accgcggcag atcgagcgat acacgttact cgcgtcagtc ggcagcgcgt tttggcaagt
     1981 gttgttcgcg accctgagtt atctggtcac ggtagcgctc ttcgtgctcg tctatcgctt
     2041 tatgccgaaa gcggaagtca ctttgcgaga cactctgcca ggcgcgtttc tcgcggggct
     2101 gctgtgggag atcgcgaagt acatcttcgc gctgagtctt cattactttc actacgatca
     2161 ggtttatgga tctgtcggcg ctgtcgttgc tgttctcacg tggagctacg tttcgagttt
     2221 gatcctgcta ttcggcgcac agctcaccgc ggtgttccat tacgagcatc ccgcaaccac
     2281 ggattaatac ggatgaaaat cgcggatttc gattatgagc tgcctgcgga tttgattgcg
     2341 cagacgccgc tggaagagcg tgatacgtcg cggatgctcg ttttggatcg gcgtgaccag
     2401 gcgtggagtg attctggatt taaagagttc acgaggtatc tgcggccgaa cgatgtcgtc
     2461 gtggtgaaca actcgcgtgt cattcccgcg cgtctactcg ggcgtcgtga ggagtcaggc
     2521 gggcaggtcg agatctttct cgtacgcgag atcgagcccg agatctggga agccctcgtg
     2581 cgtcccggcg gccgtctgaa aaaaggtgcg cgcgcagtgt ttggtgaggg caggttgatt
     2641 gctgagctgc aagacgagcc gggatcagag atgcgtcgtg tgcgttttca ttgtgacggt
     2701 ccatttcacg acgtactcgc gcagattgga tcgacgccgc tgccgccata catcaagcgc
     2761 cctgccggtg tttcgaacga agatcgcgaa cgataccaga ctgtttattc caaacagcgt
     2821 ggcgcaatcg cagcgccgac tgccggtctg catttcacgc ccgaagtgct tgaagaagtt
     2881 gcgaagattg cggggctcgc ggaagtcacg ctgcacgtcg gttatgggac gttcgaaccg
     2941 gttcgggtcg acgatgtgaa ccagcattca gtcggcagcg aacactacga gatctccgaa
     3001 gtcgcagcgc aaacgattaa cgaggcagag cgggtgatcg ttgtcgggac caccacgatg
     3061 cgagcgctcg aatcgtcagc aaacgagcgc ggcgaggtcc atgccggtcc tggcgtcgcg
     3121 agtctgacaa ttaaacctgg ctatcgtttt cgggttgcag atgcactgct taccaacttt
     3181 catctgccgc gctcgtcgct gctgattctc gtcagcgcct tcgccggtcg cgatctaatc
     3241 ttgcgcgcgt acggccacgc ggttgccgag cgctatcgct tttacagtta cggcgattgc
     3301 atgttaatcc tgtaggcaat ctgaaaaagt cgatgataaa ctaggggcgg ttttatggca
     3361 gtactaaaga aatttcgctc gcgctggtgg tcgccgtcgg atgttgaatt cgatagcgag
     3421 aagaaagaga agacaccgac gccttcgtgg ggaacgagct acgacctccg cccactgacc
     3481 atctcgcaac tggatgaggt ctggcgactc gatcagcgct gcttcatcga cggcgaggcc
     3541 tacagccgcg aaacattcga ctaccttctc accgcacctg aatcggtttc atatcgagcc
     3601 gtgacaccgg gtggcgtgat ggctgggttc gtgatcggac tcgtcgaacc ggatcacacc
     3661 gggcacgtaa cgactgtcgg cgtcgcaccc gagcaccggc gacgcagcct cgcaaagaga
     3721 ttgatgggac aggttgaaga tggctttcga aaacggaatg taagactcgt gcgactcgag
     3781 gttcggacga tgaacatcgc tgcccagaaa ctctacgaaa gcctcggcta ctcaatcaca
     3841 cagaagctgc cgaagtatta ctcgaacggc ggcgacggcc tgttgatgct caaatcctta
     3901 gaataaagtt agacaggatt acaggatttt caggattaac atgtcaatcc tgaaaatcct
     3961 gtaatcctgt ctaaaacttt tgtctaccct ttacgcagct cagtcagcac ctgcttcgcc
     4021 actgccttca acgtatcgaa cacgccctga c
//
LOCUS       DASSFQ010000296         8821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1032221, whole genome shotgun sequence.
ACCESSION   DASSFQ010000296 DASSFQ010000000
VERSION     DASSFQ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032221"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VFH96_10670"
     CDS             <1..899
                     /locus_tag="VFH96_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6974479.1"
                     /translation="GNIFIEGKPAERGENVPTAMNSSVGPGYFKTMGTPILQGREFSE
                     QDQEKTEKVAVVNEFFVRRLMPELQNPAEAVGKRFSFRSAGPFIRIVGVAQAGKYWNI
                     AEDPRPFVWTAMIQDYNSSGILVVRAKGNPEGLMGPIRGQVQSLDPNLPLFDVKTLTD
                     HMKLALFPAKVAATVLGVFGLVALMLAAIGVYGITSYAVAQRTHEIGVRLALGAQLSD
                     VLRLVLGHGLKLTIIGSVLGLFGAFLATRAITSVLYGVSATDPLTFGFVSLLLIGVAL
                     VACYVPARRATKVEPLTALRNE"
     gene            1004..3409
                     /locus_tag="VFH96_10675"
     CDS             1004..3409
                     /locus_tag="VFH96_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974480.1"
                     /translation="METLIKDLRYGVRSFLKRPGFLFIAISTLALGIGATTAMFTVVN
                     SLLLRPLNFPEPERIVRFQGINPRQGITESNMSVPDIVDWQTQSQSFEQIAGFVSGGV
                     FLNIGDETERVRATGVTTDFFPLFKTNPISGRTIQADDMKQGAEPAVVISHALWQRVF
                     GGASDVISRKISMNGKPATIVGIMPAGFTYPNDCEVWAAFALKPTEEPRDNRYVSVVS
                     RLKPGATLAQAQSEMDTISQRLSQNYGETNSGWGVRLTELRESLVGELKTSLIVLLSA
                     VGFVLLIACANVANLLLARAAARQKEIAVRTALGASRLRVVRQLLTESVLLSVVSGIA
                     GLALSIWLIKLLIAITPPNTPRVDEIGIDLRVFGFTLGVTVLAGLLFGLVPALQTSRP
                     NLNEELKDSGQRGSETSGRNRVGSLLIVSEIAISFVLLAGAGLLIKSFMHLREINPGF
                     NPDNVLVMRLTSPPGKYAQGEPRAQIYKQLVDQVKATPGVQSAGAILSLPLGGDTFNV
                     WRGLIREGRPMTPEEQTNAQHLAITPDYFQTLQIPLKAGRVFTDQDNSQSTKVVIVNE
                     KLARELWPGENPIGKRFTIWRDEKFPREVVGVVGDTRQSLDEDAGQQMYVPYAQDPTW
                     GSMSLVVRTSGEPTALAGSVREAIRAVDKAVPNYNLKTMNDVVYTSAAPRRVPMLLLS
                     VLAGAAMLLAMLGIYGVTSYYVTQRTHEIGVRMALGAQIVDVLKLVLKRAMWLALIGI
                     VIGTVGAVAVTRYMTTLLFGVKPIDVVTFVAVGAALALVVLIACVVPARRAAKIDPLE
                     ALK"
     gene            3539..5968
                     /locus_tag="VFH96_10680"
     CDS             3539..5968
                     /locus_tag="VFH96_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974481.1"
                     /translation="METLLKDIRYGIRGLLKRPGFTIIALITLALGIGANTAIFSVVN
                     AVLLRPLQFRDPEQLVVVWEEASFAGFPTNTPAPANYIDWKNQNQSFADMAAAASTSF
                     NLTGDGEPERIQANSVSANFFPLFGVQPVLGRGFLPEEDRPGGNKVAVLSYSLWQSRY
                     GGDRGIVNREILLNGQKHTVVGVMPAGFQFLEQDVRLWVPMALDQEEMANRGGHYLQV
                     IARLKPGVARSQAQADMTAVMRRIATDHPQETFDGKLGAVVMPLRDQLVGDSRSSLTV
                     LLIAVAFVLLIACANVAGLLLARAVARRREIALRMALGAGRVRVVRQLLTESLLLATV
                     AGALGSLLAYASFSFLQGLIPEQMAVATSLNLDMRILVFTLAISIVTGVVFGLVPALQ
                     AAKFDLNDALKQSSTRATASGRLRSTLIVFEVALSIVLLVGAGLLIQTLFQLFRQYSV
                     LEPEKVLTMRTILPREKYKEPQVRDNYYQQVLQRVAHLPGVVSAGYSTSVPLSWKGGS
                     SGFYPEGIKNPIPGLSYDAIHRQVSADYLKTMNIALRQGRYLDNRDNAQSMNVAVINE
                     TMARQYWPGENALGRRFKLGGPDETDLPWIQIVGIAADLRQMGLDEPVKAEMYLPYQQ
                     VDHPWFMPRDLAIRVNGDTSNLVGAVRQIIREVDPDQPVSNVATMEEVLGTEAAQRRM
                     GMIMLAGFALLALLLASLGIYGVLAYFVTQHTNEIGVRQALGATPRDILLLVLKKGMG
                     LTFVGVVIGLASAFALTRLMSSMLFGVKASDPLTFVVVPVLLSVVALLACYIPARRAT
                     KVDPLVALRYE"
     gene            6078..8516
                     /locus_tag="VFH96_10685"
     CDS             6078..8516
                     /locus_tag="VFH96_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974482.1"
                     /translation="MNTLINDLQYGLRMLWKHKSVSGIALLTLALGIGVNSAIFSVVN
                     AVVLRPLPYPESDRLMVIWGNLHKTGLEEIEASALELRDFQQQSQAFDQIAAYSIEGF
                     NLNGVDQPERLRGAAVSANLFATLRVQPQLGRNFLPDEDQLGNDTRVILGHSLWQRRF
                     GGDRNVINRSIQLDGRTLTVVGVMPAGFRFPDRDAEVWIPLAFTPDLLQEDNRGSHFL
                     NVIARLKPSVSPIQAQADLDTITARLSSEHASTYRSGFSTTIRSLHEETVGDLRRAML
                     VLLGAVGLVLLIACANVAHLRLASATSRYREFAIRSALGANRTRVVRQFLTESLLLSF
                     IGGAIGLALAVWVVRALVVLMPKDTPRLEEIALDYRVVLFTLGISILTGIVFGLAPSF
                     QAARTNLNDVLKEAGRGGGDGSRRLRLRNLLVVSEFALSLVLLISAGLMIKSLLRLQE
                     VKPGFDSANLLTMRIALPASKYDKFDKSHAFFQQLFDQLSARPEIESVGAINLLPFGG
                     GGGDRSFSIEDQPTPSGHSRPDEQVRFVTPGYFGAMSILLLSGRDFTRYDLPDKPQVA
                     IVNSAFAHKFWPSGNALGKRISFQNKNPKWYEIVGVVGNVKHRGLDIQDNPELYIPAF
                     QPLFADGNVPALYLAVRTASDPLQLGPAMRGEVAALDRDQPIYSMMSMDQRISDSIAP
                     RRFNMFILGLFAVLALVLAAIGIYGIMAFSVVQRTHEIGVRMALGASTQDVLKLVMRN
                     GFKLALIGIAVGLVAAFAATRVLSSLLFEVSARDPLIFVIDAVLLAIAALLACYIPAR
                     RATKVDPLVALRYE"
     gene            8574..>8821
                     /locus_tag="VFH96_10690"
     CDS             8574..>8821
                     /locus_tag="VFH96_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974483.1"
                     /translation="MNTLFQDLQYGFRMLAKHKSVTAIAVLTLALGIGANTAIFSVVN
                     AVLLNPLPYREPDRLVSLWENVPGHGRWRASPANFFDW"
BASE COUNT         1736 a         2280 c         2711 g         2094 t
ORIGIN      
        1 gtggcaatat tttcatcgaa ggaaagccgg cggaacgcgg cgagaacgtg cccacggcaa
       61 tgaattccag tgttgggcca ggctacttca agacgatggg tacaccgatt ctacaggggc
      121 gcgagtttag cgagcaggac caggagaaaa cagaaaaggt tgcagtcgtt aatgaatttt
      181 ttgtgcgacg tctgatgccc gaattacaaa atccggctga agctgtaggc aagcgcttca
      241 gttttcggag cgccggacca tttattcgca tcgttggggt ggcacaagcc ggtaagtatt
      301 ggaacatcgc cgaagatccg cgcccgtttg tctggacagc gatgattcag gattacaact
      361 cgagtggcat tctcgtagtg cgcgcaaaag gaaacccgga agggttgatg ggaccaattc
      421 gcgggcaggt gcagtcgctc gatccaaact tgccgctatt cgacgtcaag acgctgacgg
      481 accacatgaa gcttgcgttg ttcccggcga aagtcgcggc gacggtgctg ggagtgtttg
      541 gtctcgtcgc gctcatgctg gcggcgatcg gagtttacgg aatcacgtcg tatgcagtcg
      601 cgcaacgtac acatgagatc ggcgtgcgac tcgcgctcgg cgcgcagttg agcgatgtgc
      661 tgcggctcgt gcttgggcat gggttgaagc tgacgatcat cggttcagtg cttggactgt
      721 ttggcgcttt tctcgcgacg cgtgcgatca cgagtgtgct ttatggcgta agcgcgactg
      781 atccgctgac gtttggtttt gtgtcgctgc tgctgatcgg cgtcgccctc gttgcgtgtt
      841 acgtaccggc acgacgagca acaaaggtcg aaccacttac ggcgttgcgg aatgagtgac
      901 ctccttggag tgcggtgact tgtcaccgct ttggtaaaca gtaccaaagc gccaacaagt
      961 tggcgcactc caaggagacg ccacatgctg tggagtcaaa ggtatggaaa cactgattaa
     1021 agacctgcgt tacggcgtgc gcagttttct caaacgcccg gggttcctgt ttattgctat
     1081 ttccacgctc gcgctcggta tcggcgcgac gacggcgatg ttcacggtgg tgaattcact
     1141 gttgttgcgg ccactgaatt ttccggagcc agagaggatt gtgcgttttc agggaatcaa
     1201 cccgcggcag ggaatcactg agagcaacat gtctgtgcct gacatcgtag actggcaaac
     1261 acagagccaa tcgttcgaac agatcgcggg ttttgtctcc ggcggcgtgt tcctcaacat
     1321 cggcgacgag accgagcgcg tgcgtgccac gggggtgacg accgacttct ttccgctttt
     1381 caaaacgaat ccgatcagcg gtcgtacgat acaagctgat gacatgaagc aaggcgccga
     1441 gcctgcggtc gtgatcagcc acgcgttgtg gcaaagagtc tttggaggag cgtcggacgt
     1501 catcagccga aagatctcaa tgaacggtaa gcccgcgacc atcgttggca tcatgccggc
     1561 cggcttcacg tatccgaacg actgcgaagt ttgggcagcg ttcgcgttaa aaccaaccga
     1621 agagccgcgt gacaatcgat acgtttcggt cgtgtcgcgg ttgaagccgg gcgctacgct
     1681 ggcgcaagcg caatcggaga tggacaccat tagccagcgt ctgtcgcaga actacggtga
     1741 gacgaacagc ggttggggcg ttcgacttac cgagttacgc gagagcctcg tgggtgagtt
     1801 aaagacgtcg ctgattgtcc tcttgagcgc agtcggtttc gtgctgttga ttgcgtgtgc
     1861 aaacgtcgcg aatctgctgc tggcacgcgc cgcggctcgg caaaaggaga ttgctgttcg
     1921 aactgccctc ggtgcgagtc gcttgcgcgt cgtccggcaa ctgctgacgg agagtgtttt
     1981 gctttcggta gtgagtggca tcgccggact cgctctcagt atctggttga tcaagctgct
     2041 gattgcgatc acgccgccga atacaccacg cgtcgacgag atcggaatcg atctgcgtgt
     2101 gtttggattc actctcggcg ttactgtgct cgcgggtctt ttgtttggac tcgttcccgc
     2161 attgcagacg tcgcggccca accttaacga agagctcaaa gacagcggtc aacgcggttc
     2221 ggaaacaagc ggacgcaatc gcgtcggtag tttgttgatt gtttcggaga tcgcaatttc
     2281 gttcgtgctg cttgccggcg ctggattgct gatcaagagc ttcatgcacc tccgagagat
     2341 caatcccggt ttcaatcctg ataacgttct ggtgatgcgg ctgacttcac cgcccggaaa
     2401 atacgcacag ggcgagccgc gcgcgcagat ctacaagcaa ctcgtggatc aggttaaggc
     2461 aacgcccggc gtgcagtccg ccggcgcgat cctgagtctg cctttgggtg gcgacacctt
     2521 taatgtttgg cgcggactga ttcgcgaggg ccggcccatg acgcccgagg agcaaacaaa
     2581 cgcgcagcat ctcgcgatca cgcctgatta ctttcagacg cttcagattc cgctgaaagc
     2641 cggacgggtt tttaccgatc aggacaactc gcaaagcacc aaagtcgtga tcgttaatga
     2701 aaaattggca cgcgagttgt ggcctggaga gaatccgatt ggcaagcgct tcacgatttg
     2761 gcgtgatgaa aagtttcccc gcgaagtcgt cggtgtcgtc ggcgacacca ggcaatcgct
     2821 cgacgaggac gcgggacagc agatgtacgt gccttacgcg caggatccga cgtgggggag
     2881 tatgtcgtta gtggtgcgca cgagtggtga accgaccgcg ttggccggat cagtgcgcga
     2941 agcgattcgc gccgttgata aggccgtgcc gaactacaac ctgaagacga tgaacgacgt
     3001 ggtttacacg tcggcggcgc cgcgacgcgt gccgatgttg ttgttaagcg tgcttgcggg
     3061 tgcggcgatg ttgctcgcga tgctcggaat ttatggcgtc acgtcgtact acgtcacgca
     3121 aagaacacac gagattggtg tgcgcatggc gcttggggca cagatcgtcg acgtgctcaa
     3181 gctcgttttg aaacgcgcga tgtggctggc gttgatcggt atcgtcatcg gcactgtggg
     3241 cgctgtggcg gtgacgcgtt acatgacgac gttgctgttt ggggtgaagc cgatcgatgt
     3301 ggtgacgttt gtggcggtgg gcgcggcgct ggcgttggtg gtgttgattg cgtgtgtggt
     3361 tccggcgcgc cgggcagcga agatcgatcc gctcgaagca ctgaagtaga tcagctttgg
     3421 gcgcctcctt ggagtgcggt gacttgtcac cgctttggta aaaccgtacc aaagcgccaa
     3481 caagttggcg cactccaaag tgacgccaga tttacgcggg ccgtggtgtt tttagatcat
     3541 ggaaacatta cttaaagaca ttcgctatgg tattcgtggt ctgttgaagc ggcccggatt
     3601 tacgattatt gctctcatca ctctcgcgct cggaattggc gccaacactg cgatcttcag
     3661 cgtcgtcaat gcggtgctgt tgcgtccgct tcagtttcgc gatccggaac agttagtagt
     3721 cgtctgggaa gaggcttcat tcgccggctt tcccacgaac acgccggcgc cggccaacta
     3781 catcgattgg aaaaatcaga accagtcgtt tgcagacatg gctgcggctg cgtcgaccag
     3841 cttcaatctt accggcgacg gcgagcccga acgcattcag gcaaattcag tcagtgcaaa
     3901 cttcttcccg ctcttcggcg tgcagccggt gctcggtcgc ggttttcttc ccgaagaaga
     3961 tcggcccggc ggaaacaagg ttgccgtgct cagctacagt ctctggcaga gccgttacgg
     4021 cggtgaccgc ggcatcgtca atcgcgagat cctactgaat ggacaaaagc acaccgtagt
     4081 cggcgtgatg ccggctggtt ttcagtttct cgaacaagat gtgcgtttgt gggtgccgat
     4141 ggcgttagac caggaagaga tggcgaatcg cggtggtcat tatttgcagg ttatcgcgcg
     4201 cttgaagccg ggcgtggcta ggtcgcaagc gcaagcggat atgaccgccg tcatgcgacg
     4261 gattgccacg gaccatccgc aagagacatt tgacggcaaa ctcggcgcgg tcgtgatgcc
     4321 gctgcgcgat cagttggttg gcgactcgcg cagctcgctg acggtgttgt tgattgcggt
     4381 tgcttttgtg ttgctgatcg cgtgtgcgaa tgtcgccgga ctcttgctgg cgcgcgcggt
     4441 tgctcgccgt cgcgagatcg ctttgcgaat ggcgttaggt gcgggccgtg ttcgagtggt
     4501 caggcagtta ctgactgaga gtttgttact ggcaactgtt gccggtgcgc tcggatcgct
     4561 tttggcgtat gcgagtttta gttttcttca gggtttgatt ccggagcaga tggctgttgc
     4621 gacgagtcta aatctcgaca tgcgcattct tgttttcacg cttgcgatct cgattgttac
     4681 gggagtcgtc ttcggtcttg tacccgcgct gcaagcggcg aagtttgatc tgaacgatgc
     4741 gttgaagcag agcagcacac gcgcgacggc gagtggtcgt ttgcgcagca cgctgattgt
     4801 ttttgaagtg gcgttgtcga tcgtgcttct agttggcgcg ggtctgctga tccaaacgct
     4861 gtttcaacta ttccggcagt attcagtgct cgaaccggaa aaggttctga ccatgcgcac
     4921 gatcctgccg cgcgagaaat acaaggagcc gcaagtgcgc gacaactatt accagcaggt
     4981 gctgcaacgc gtcgcgcatc tgccgggcgt agtgtctgcg ggttacagca cttccgttcc
     5041 gctttcgtgg aagggtggtt ccagtggttt ctatccggaa gggatcaaga atccgattcc
     5101 gggtttgtca tacgacgcga ttcatcgcca ggtaagtgct gactatctga agaccatgaa
     5161 tatcgcgttg cgacagggtc gttacctcga caatcgcgac aatgcacagt caatgaatgt
     5221 tgcggtcatc aacgaaacga tggcgcgaca atattggccg ggtgagaacg cgctcggccg
     5281 tcgtttcaag ctcggtggtc ctgacgagac tgatctaccg tggattcaaa tcgttgggat
     5341 cgctgccgat ctgcggcaaa tgggattgga tgagccggtc aaggctgaga tgtatctgcc
     5401 ctatcaacaa gtggatcatc catggttcat gccgcgtgac ctcgcgattc gcgtcaacgg
     5461 cgatacgtcg aatctggttg gcgccgtgcg gcagatcatt cgtgaagtcg atccggatca
     5521 gccggtgtca aacgttgcga cgatggaaga agtgctcggc actgaagcag cgcagcgacg
     5581 gatgggaatg atcatgctgg ctgggttcgc tttgctggcg ttgttgctgg cgtcgctcgg
     5641 gatttacggc gtgctcgcgt acttcgtcac acaacataca aacgagattg gcgtgcgaca
     5701 ggcacttggc gcgactccgc gcgacattct gttgctggtg ttgaaaaaag gaatgggttt
     5761 aacgttcgtt ggcgttgtga tcggacttgc gagtgcgttt gcgctgacgc ggttgatgag
     5821 ctcgatgctg tttggagtga aggcgtcaga tccgttgacg tttgttgtgg tgccggtgtt
     5881 gctgtccgtc gtggcgttgt tggcttgtta catccctgca cgccgcgcga cgaaggtcga
     5941 tccgctcgtt gcattaaggt acgaataact tcaggcgcca ccttggagtg cggtgacttg
     6001 tcaccgcttt ggtaaacagt accaaagcgc caacaagttg gcgcactcca aggtggcgtc
     6061 caattcgacg attcgtgatg aatacgctaa tcaatgatct tcaatatggc ctgcgaatgc
     6121 tgtggaagca caagagcgtc agcgggattg cgctgcttac attggcgctt ggtattggtg
     6181 tgaatagcgc catcttcagt gtcgtcaacg ctgttgtgtt gcgtccgctg ccgtatcccg
     6241 agtcggaccg actgatggtg atttggggca atttgcacaa gaccggtctc gaagagatcg
     6301 aggcctcggc gctcgaactc agagactttc agcaacagtc ccaggcattc gatcagatcg
     6361 ctgcttattc gattgaaggt ttcaatctta acggtgtcga tcaacctgag cgattgcgcg
     6421 gcgcggctgt ctcagcgaat ttgtttgcaa cgctgcgcgt gcaaccgcaa ctcggtcgta
     6481 acttcctgcc cgatgaagat caacttggca acgacacacg cgtgatactc ggccactcgc
     6541 tatggcagcg acgatttggc ggcgaccgga acgttatcaa tcgctcgatt caactcgacg
     6601 gccgcacgct gacagttgtc ggcgtgatgc cggcagggtt tcgctttccc gaccgagacg
     6661 cggaagtttg gattccgctc gcgtttacgc ccgatctgtt gcaggaagac aaccgtggct
     6721 ctcatttcct gaatgtaatc gcgcgtctga aacccagcgt gagtccgatc caggcgcagg
     6781 cagatctcga cacgataaca gcacgacttt cgagtgaaca cgcttcaacg tatcgcagtg
     6841 gtttcagcac gacgatccgt tcacttcacg aggaaacagt cggcgacttg cgacgcgcga
     6901 tgctggtgtt gctcggcgcc gttgggctcg tgttgctgat tgcgtgcgcg aacgttgcac
     6961 acctgcgact cgcaagcgcg acgtcgcgtt accgcgagtt tgcgattcgc tctgcgttgg
     7021 gagcaaaccg cactcgcgtg gtccgtcagt ttttgacgga gagtctgctg ctttcattca
     7081 ttggtggagc gatcggattg gcgctggctg tttgggttgt gcgagcgctc gtagtcctga
     7141 tgccgaagga cacgcctcgt ttggaagaga tcgcgctgga ttatcgcgtt gtccttttca
     7201 cactcggcat ctcgatcctc actggcatcg tgtttggact ggcgccgtcg ttccaggccg
     7261 cacgcacgaa cctgaacgac gtcttgaagg aagccgggcg cggaggcggc gacggttcgc
     7321 gacgacttag gctgagaaat ctgttagtcg tttcagagtt tgcgctttcg ctcgttttgc
     7381 taatcagcgc gggactcatg atcaagagct tgttgcgttt gcaggaggtc aaaccgggtt
     7441 tcgattccgc gaacctgctg acaatgcgca tcgcattgcc tgcaagcaag tacgacaagt
     7501 tcgataagag tcatgcattt tttcagcaac ttttcgatca gttgtcagca cgaccggaga
     7561 tcgagtctgt cggcgcgatc aacttactgc cgttcggtgg cggtggtggc gaccgcagct
     7621 tttcgatcga ggatcaacca actccttcgg gacactcgcg accggacgag caagtgaggt
     7681 ttgtcacgcc cggttacttc ggcgccatgt cgatcctgtt gctcagcggt cgcgatttta
     7741 cgcgttacga tttacctgac aagccacagg tcgcaattgt gaacagtgcg ttcgcgcaca
     7801 agttctggcc cagcggcaac gccctcggca agcgcatttc atttcagaat aaaaatccga
     7861 agtggtacga gattgtcggt gtggttggaa acgtaaagca tcgcggtctc gatatccagg
     7921 acaatccgga gctttacatc cctgcatttc agccgctgtt tgccgatggc aacgtcccgg
     7981 cgctttatct ggcagtgcgc accgcgagtg atccgttgca gcttgggcct gccatgcgcg
     8041 gtgaagtggc ggcgctcgac cgcgatcagc cgatctacag catgatgtcg atggaccagc
     8101 gaatctcaga ttcgatcgcg ccgcgtcgtt tcaacatgtt cattctcggc ttgtttgctg
     8161 tgctggcgtt agttctcgct gcgattggaa tctacggcat catggcgttc tcggtggtcc
     8221 agcggacgca cgagattggc gtgcgtatgg cgctcggtgc aagcacgcag gacgtgttga
     8281 agctggtgat gcgaaatgga ttcaagctgg cgttgattgg gattgctgtc ggtctggtcg
     8341 cagcgtttgc ggccacgcgt gtgctctcgt cgttactctt tgaagttagc gccagagatc
     8401 cgctgatctt tgtgatcgac gcggtgttgc tggcgattgc cgcgttgctg gcctgttaca
     8461 tccccgcgcg ccgcgcgacg aaagtcgatc cgctcgtggc gctgcgatac gagtgaaagg
     8521 ccatccacag attacgcaga taatctgtgt aatctgtgga tggatcgatg atcatgaaca
     8581 cactcttcca ggatcttcag tacggctttc gaatgctcgc gaagcataag agcgtcaccg
     8641 cgattgccgt gttgacgctc gcactcggca tcggcgcgaa cactgcaatc ttcagcgtcg
     8701 tgaacgcggt gttgctgaat ccgctgccgt atcgcgaacc cgatcggctt gtgtctcttt
     8761 gggaaaacgt gccgggtcac ggtcgctggc gcgcttcacc cgcaaacttt ttcgactgga
     8821 a
//
LOCUS       DASSFQ010000297         4411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_288509, whole genome shotgun sequence.
ACCESSION   DASSFQ010000297 DASSFQ010000000
VERSION     DASSFQ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288509"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFH96_10695"
     CDS             <1..450
                     /locus_tag="VFH96_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET6974484.1"
                     /translation="DEERAKFEEWIKTTYYQDFVPKVAKGRHKDAQYIDSIAQGRVWT
                     GGQAKERGLVDEFGGLDKAIEVAKELAKIPADKGVERVILPYPRTFLQELLSNDGDNS
                     NTQVQQQRAVYAALPEDARRAFRYMQLMDRMKNGETMFVMPFDIRIK"
     gene            complement(478..1758)
                     /gene="serS"
                     /locus_tag="VFH96_10700"
     CDS             complement(478..1758)
                     /gene="serS"
                     /locus_tag="VFH96_10700"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET6974485.1"
                     /translation="MLDLNYVRENIDKVRASLEARGADTRALDDFAQADEERRRTIAE
                     SDQLNAQRNASSREIGGLMKEGKKDEAEARRAEVSGLKDRISELDGLRAQTEERMREL
                     LSTLPNVPHSSVPVGKDESANTEVRRWGTRPEFSFEPKDHVDLGTTLGILDLERATKI
                     ASARFAILNGAGARLERALINFMLDVQTREHGYLETLPPFIVNRHSLFGTAQLPKFEA
                     DLFKLEDPRELYLIPTAEVPVTNYYRDEILDASQLPMKWAAYTPCFRSEAGSYGRDTR
                     GVIRQHQFEKVELVKYALPENSYEELEALTRDAETILQKLGLHYRVVTLSTGDLGFGS
                     AKTYDIEVWLPSQNAFREISSCSNYESFQARRAQIRFRRSGGAKPEFVHTLNGSGLAI
                     GRTWVAILENYQQEDGSVTVPKILRPYMGTDRIS"
     gene            complement(1821..2477)
                     /locus_tag="VFH96_10705"
     CDS             complement(1821..2477)
                     /locus_tag="VFH96_10705"
                     /inference="COORDINATES: protein motif:HMM:NF016581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HET6974486.1"
                     /translation="MDGITFDKRFFDSTRGQIVTLLRSAPCTVEELAGKLKLTDNAVR
                     AHLATLERDGLVKQSGLRRGPRKPHFTYVLTEESDKLFPKAYDALLNQLIAVLKTRLE
                     PAEIEDVLREVGRALAADAPSGQHETLENRVKIALKVLEAIGGAAEIEQHGDKIVIVS
                     GGCPLAAAVSVHPEVCRLAETLVAEIVKVPVEERCDRTGRPKCRFELATKGTKSTKVD
                     "
     gene            2604..3026
                     /locus_tag="VFH96_10710"
     CDS             2604..3026
                     /locus_tag="VFH96_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974487.1"
                     /translation="MAQAISSSAGVASRTSESVSSPGYQAFQILRTGFTVAPIVAGLD
                     KFFHLLVNWDQYLPSFVNNMTGGHGHQLMLAVGVIEIVAGLGVAFKPRLFAYVVSAWL
                     LLIVANLLMIPGYFDVALRDFGLSLAALALARLSQEYA"
     gene            3038..4411
                     /locus_tag="VFH96_10715"
     CDS             3038..4411
                     /locus_tag="VFH96_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974488.1"
                     /translation="MNPSKGRLILAERGLARIALVLFVAAVTLVFVSTRDSWAEGEDR
                     GKSTASERQTDANAQRMLDEGKQTFRFDTFGDEAFWTDALQLNRAIAGANFGGVGPGV
                     SPATALAVGLKVDTDALPQSLISQLKHGRVNLDDPATTLALLKLNAVVGVKGTFKGDG
                     SLQSVGITCALCHSTVDNSFAPGIGQRLDGWANRDLNVGAIVALSPNLTPVATLLGVD
                     VPTVKTVLQSWGPGKFDAELFLDGKAFRPDGKSAATLIPPAFGLVGVNLHTWTGWGSV
                     THWNAFVANLEMHGQGTFYDPRLNDPVKFPIAAREGFGNVRSNPDLISPKLPALHFYQ
                     LAIPAPAAPAGSFDPAAAARGAALFNGQAKCAVCHVPPTFTEPGWNMHTAEEIGIDDF
                     QAKRSPDERYRTSPLKGLWTHQKGGFYHDGRFATLRDVIEHYNAFLGTNLTDQQKSDL
                     VEYLKSL"
BASE COUNT          944 a         1233 c         1248 g          986 t
ORIGIN      
        1 gacgaagagc gcgcgaagtt tgaagagtgg atcaagacga cttattacca ggattttgtg
       61 ccgaaggtcg cgaagggacg ccacaaagac gcgcagtata tcgactcgat cgcccaggga
      121 cgcgtgtgga ccggcggcca ggcgaaggaa cgcgggctcg ttgatgagtt cggcgggttg
      181 gataaagcga tcgaagtggc gaaagagctt gcgaagattc cggctgacaa aggcgtcgag
      241 cgcgtgatcc tgccttaccc gagaacgttc ctgcaggagt tgttgagtaa tgacggcgac
      301 aattccaaca cgcaagtcca acaacagcgc gcagtttatg cggccttacc cgaagatgcg
      361 cgacgcgctt ttcgctacat gcagctgatg gaccggatga agaatggcga gacgatgttc
      421 gtgatgccat tcgatatccg cattaagtaa tccgcgtttt atccgcgttc atccgcgtta
      481 actaatgcgg tcggtgccca tgtacggtcg tagaatttta ggcacggtca ccgacccatc
      541 ttcctgctgg taattctcca gaatcgcgac ccacgtgcgt ccaatcgcca atccggatcc
      601 gttcagcgtg tgtacaaact ccggcttcgc cccaccgctt cgccggaaac ggatctgagc
      661 acggcgcgcc tggaacgatt cataattcga acaagacgag atctcacgaa acgcattctg
      721 cgacggcagc cacacttcga tgtcgtaagt cttcgccgat ccaaacccga gatcgccagt
      781 cgagagcgtc acgacacgat aatgcaatcc cagtttttgc aggatcgtct ccgcgtcgcg
      841 cgttagcgct tcaagctcct cgtaagagtt ttccggcagc gcatatttca cgagctcgac
      901 tttctcaaac tggtgttgcc tgatcacgcc gcgcgtgtcg cgcccatagc tgcctgcctc
      961 cgatcgaaag cacggcgtgt atgcggccca cttcatcggc agttgcgacg catcgagtat
     1021 ctcgtctcga taataatttg tgaccggcac ttccgccgtc ggaatcaaat aaagctcacg
     1081 cggatcttcg agcttgaaga gatcggcctc aaactttggt agctgggccg tgccaaaaag
     1141 tgaatgacga ttgacgatga atggcggcaa cgtttccaga taaccgtgct cgcgcgtctg
     1201 cacgtcgagc atgaaattga tgagggcgcg ttcgagtcgt gcgcccgcgc cgttcagaat
     1261 agcgaagcgt gcggatgcga tcttcgtggc gcgttcgaga tcgagtatgc cgagcgtggt
     1321 gcccagatcg acgtggtctt tcggttcgaa agaaaactcc gggcgcgtgc cccagcgacg
     1381 gacttcggtg ttggcagact cgtctttgcc aaccggaact gacgagtgtg gaacgtttgg
     1441 cagcgtcgag agcagctcgc gcatgcgttc ttcggtttgc gcgcgcagcc cgtcgagctc
     1501 gctgatacga tctttcaacc cactgacttc ggcgcgacgc gcttcggctt cgtccttctt
     1561 gccttccttc atcaagccgc cgatctcacg actcgacgcg ttgcgctgcg cgttgagctg
     1621 atccgattcg gcaatcgtgc ggcgccgctc ttcatcagcc tgcgcaaaat catcgagcgc
     1681 tcttgtgtca gcgccacgcg cttcgagcga cgcgcgcact ttatctatat tttccctgac
     1741 gtagttgaga tccagcataa aagtatgtag ccacaaaagg cacaaaaagc acaaatgctt
     1801 tctttgacct cgaggcgcgc tcaatcaact tttgtgcttt ttgtgccttt tgtggctaat
     1861 tcgaatcggc acttgggacg gccagtgcgg tcgcaacgtt cttcgacagg gactttaaca
     1921 atttcggcga cgagagtctc ggcgagtcga caaacttccg gatggacaga cacagcagcg
     1981 gccagtggac agccgccaga tacgatcacg atcttgtcac cgtgctgctc gatttcagcc
     2041 gcgccgccaa tcgcctcgag gaccttcaac gcaatcttca cacgattttc cagggtctca
     2101 tgctgtccgc tcggcgcatc tgcggcgagc gcacgcccga cttcacgcaa aacatcctcg
     2161 atctccgccg gctccaaacg tgttttaaga accgcgatga gctgattgag tagcgcgtcg
     2221 tacgctttcg gaaagagctt gtcagattct tcggtgagta cgtaggtaaa gtgtggtttg
     2281 cgcggaccgc gtcgcaggcc gctttgtttg acaagcccat cacgttcgag agtcgcgagg
     2341 tgcgcgcgaa cggcgttgtc agtgagcttg agttttcccg ccagctcctc gacggtgcac
     2401 ggcgcgctgc gcaaaagcgt aacgatctgc ccgcgcgtac tgtcgaaaaa gcgtttatcg
     2461 aacgttattc cgtccatctg gctcgcgatt ctaacgcatt tctaaagaaa acacaaaaaa
     2521 tcaaataagt tcttgactta ttatatgaca ggagtatttt attgagctca ggaccaaatc
     2581 tctaatcagg agcactaaag acaatggcac aggcaataag tagttcagcg ggggtcgcct
     2641 cgagaacgag cgaaagcgtt agtagccccg gctatcaggc gtttcagatt ctgcgcaccg
     2701 gcttcaccgt cgccccgatc gtggccgggc tcgacaaatt ttttcacctg ctggtgaatt
     2761 gggatcaata ccttccttcg ttcgtcaata acatgaccgg cggtcacgga caccaattga
     2821 tgctcgccgt cggtgtgatc gaaatcgtgg caggccttgg agtcgcattc aaaccacgcc
     2881 ttttcgcgta cgtcgtctcg gcatggctgc tgttgatcgt ggccaatctg ctgatgattc
     2941 cgggttattt cgatgtggcg ctgagggatt tcggactctc cctcgctgca cttgcactgg
     3001 cccgtctcag tcaggaatac gcataaggag gatgatcatg aaccccagca aagggcgact
     3061 cattttggcc gagcgcggcc tggcgcgaat cgcgttagtg cttttcgtcg ccgccgtaac
     3121 gttggttttt gtaagcacga gggacagttg ggcagaaggc gaggatcgcg gcaaaagcac
     3181 ggccagcgaa cggcagacgg atgctaacgc gcagcggatg ctcgacgaag gcaagcagac
     3241 ttttcgtttc gacacgtttg gagacgaggc gttctggact gacgcgctgc aactcaatcg
     3301 cgcgattgcg ggggcgaatt ttggtggcgt cgggccgggc gtcagcccgg cgacggcact
     3361 cgccgtcggt ctgaaagttg acacagacgc gctgccacag tcactcattt cgcagttaaa
     3421 acatggccgc gtgaatctcg atgatccggc tacgacactc gcgttgctca aactcaacgc
     3481 cgtcgtcggt gtgaaaggta ctttcaaagg cgacggcagt cttcagtcag tcggaatcac
     3541 ctgtgcgctc tgtcactcga ctgtagataa ctcattcgcg cccggtatcg gccagcgtct
     3601 tgacggctgg gccaatcgcg atctcaacgt cggcgcgatt gttgcgctga gtccaaatct
     3661 cacgccggtt gcaaccctgc taggcgttga tgttccgaca gtgaaaacag ttttgcagag
     3721 ttggggaccg ggcaagttcg atgcggagct gtttcttgac ggcaaggctt tcaggcccga
     3781 cggcaagtca gcagcgacgt tgatcccgcc ggcgtttggt ctcgtgggcg tcaacctgca
     3841 cacctggact ggctggggat cggtcacaca ctggaatgcg ttcgtcgcaa atctcgagat
     3901 gcacgggcag ggaacgtttt acgatccgcg tctcaacgat ccggtgaagt ttccgatcgc
     3961 cgcgcgtgaa ggtttcggaa atgtgcgtag caatcccgat ttgatcagtc caaagcttcc
     4021 tgcgcttcac ttctatcagc tcgcgattcc tgcacccgcg gcaccggcgg gtagtttcga
     4081 cccggcggca gcggcacgcg gtgcggcgct gttcaatggt caggcgaagt gtgcggtttg
     4141 tcacgtgccg ccaaccttca ctgagccggg atggaacatg cacacggcgg aagagatcgg
     4201 tatcgacgac tttcaggcca aacgctcgcc tgatgaacgt tatcgaactt ctccgttgaa
     4261 gggactctgg acacaccaga aaggcggctt ctatcacgac ggacgcttcg ccacgctccg
     4321 cgacgtgatc gagcactaca acgctttttt gggtacgaat ttgacagacc aacagaaatc
     4381 cgatctggtc gaatatttga agtcactcta a
//
LOCUS       DASSFQ010000298         5018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_579236, whole genome shotgun sequence.
ACCESSION   DASSFQ010000298 DASSFQ010000000
VERSION     DASSFQ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579236"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFH96_10720"
     CDS             <1..1345
                     /locus_tag="VFH96_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HET6974489.1"
                     /translation="TGDETYGYSSGLADLDEMLGSAVMSEIFYRQGIPTERCLAVIGF
                     SDTSAIGVRTAPNLIRPAHMFRYLKQGRHEELKASVDYFIDREVENGFWEIPDDSSER
                     YTRALGYFARSYAKLAAILEEEYIFNWLSWDGDNMLASGAILDYGSIRQFAAKHDKYR
                     YNDVDRYSASLAEQRGWARSLVQVFAQTMAFVQSGVKENLNNFKNAECLRVFDEAFEN
                     ERDQRLLWRIGFTSEQISYLMSNASKEIRDFDRSLRYFEDRKVKKGIEKLPDGFTHSP
                     VFLIRNLLRLLPAYYVAQTITRADEQSAYMPEEIFCRIMAASYISKRDLKPTASTMSH
                     VEDFQHCYLKLVAALGGPFDLVLKTLQERSAVINHRHRITGDAVTWIIEEVIAMKNKI
                     RIDGLQEALDAFIDSQVLIPGKWHPVPAEQLKSNTLKSQLLQKIHENLEMYKESI"
     gene            1351..2475
                     /locus_tag="VFH96_10725"
     CDS             1351..2475
                     /locus_tag="VFH96_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6974490.1"
                     /translation="MINWAEVRAKFPVTSGSVYLNTAAAGPLADSTARAGELYYEQMM
                     NDGDVHWDEWLARCEDIRKQVAAFINAEPEEIGFTTNTSSGMNLIVDALEQHGEVISC
                     DLEFPVTTIPWMHRRIPVHFVKSIDGEVRPKDVRAAMNTRTGIISMSYVQFSNGFRSD
                     LEELASIKGNHALVVNASQAAGAFEIDVKRMKIDALCSTGHKWMRSGYGSGFVYISKE
                     LQAASRQRIIGWLSVHDPYGMRNDEIHLRHDVASRAEIGCLHFAGVFALGASIELMQS
                     IGIENIQARVLELNQKLTSRLDEIGWKVLSPLGEDKFRSAETLVAADEPGKIVAQLAQ
                     QKVLVTEKPQGFRVSTDLFNNEEDIEKLAHGLHGFQTDYQ"
     gene            complement(2487..3434)
                     /locus_tag="VFH96_10730"
     CDS             complement(2487..3434)
                     /locus_tag="VFH96_10730"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6974491.1"
                     /translation="MEIRQLRAFVAIAESGTFTAGALRVHVTQAAISMQIRQLETEIG
                     AKVFVRAPRHVILTEAGEQLLRRARHILREHDAALDEIAELAGAERGRLRIGSASAMV
                     LTEQLPSILKELRKQHPAADISVTSGTSEVLVDQILAGEVDVAFVSLPVDVRGIKTER
                     LSEDQLVAIASPRHKLAKQRTISAYTLAGERLILGERGGNTRRLIDQFFAQAGVSLRV
                     AMELSRQQAIKRMVEEDMGVGIVPLQSVKEEVEKGRLIRWWIEGAQINWELGIAQLSN
                     GYDSPIMQKFVALAHRNFNDSNSAGASKKTKKPGHRLHR"
     gene            3615..4562
                     /locus_tag="VFH96_10735"
     CDS             3615..4562
                     /locus_tag="VFH96_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET6974492.1"
                     /translation="MLTQNSVVGVDGGGTKTEAVVMDANQRVIGEGRAGPSNPLRVGI
                     AGAAAAVREAIDSACAAAKLRRGDLIAAQIGLAGARRRELRERMRETLMPLGVGEVEV
                     VTDATIALYGATDGAPGLVVIAGTGSICCGINARGKRFCAGGWGPIAGDEGGGAWLAR
                     RALRAVAYAFDGRGPKTDLTELACSYFHVTSPDDLTTAIYAPTITNERLAGFGRDVVD
                     AAKSNDPVAHQIVADGGTELGHAAVAVIRNLQMERERFQIAYVGGVFRAAGDLMLDTL
                     RAEVRKVAPRAYFEPPHFAPAVAAARMARDRINHVALAV"
     gene            4564..>5018
                     /locus_tag="VFH96_10740"
     CDS             4564..>5018
                     /locus_tag="VFH96_10740"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HET6974493.1"
                     /translation="MKTETALPITEQENPRSKDLSSQSTAQIVALMNDEDATVAAAVK
                     RVLGDVVKAVDEIVVRLAKGGRLFYTGTGTSGRLGVLDASECPPTFGVSPDLVQGVIA
                     GGYDACHRAVEASEDDAEAGGFDLKSRGFGTGDVLVGIAASGRTPYTVGA"
BASE COUNT         1111 a         1361 c         1459 g         1087 t
ORIGIN      
        1 gaccggtgac gaaacttacg gttactcatc aggcctcgca gatctcgacg agatgcttgg
       61 cagcgcggtg atgtctgaga tcttttatcg tcagggaatt cccaccgaac gctgtctcgc
      121 cgtgatcgga ttttctgaca cgtctgcgat cggcgtgaga accgctccga atctcatccg
      181 cccggcgcac atgtttcgct atctcaagca gggccgtcac gaggagctga aagcgtcagt
      241 cgattatttc atcgatcgcg aagtcgaaaa tggcttctgg gaaattccgg acgattcgag
      301 tgagcgttac acaagagccc tcggttactt cgcgcgctca tacgcaaaac tcgcggccat
      361 tctcgaagag gaatacatct tcaactggct ctcgtgggac ggcgacaaca tgctcgcgag
      421 cggcgcgatt ctcgactacg gctcgattcg acagtttgcg gcgaagcacg acaagtatcg
      481 ctacaacgac gtggatcgtt attccgcttc actcgccgaa caacgtggct gggcgagatc
      541 gctggtccag gtctttgcgc aaaccatggc tttcgtccag tcaggcgtaa aagagaactt
      601 gaacaatttc aagaacgccg agtgcctgcg cgtcttcgat gaagctttcg aaaacgaacg
      661 agatcagcgt ctgctctggc ggatcggttt cacttcggaa cagatctcat acctgatgag
      721 caacgcaagc aaagagatca gggatttcga tcgcagcctg cgctacttcg aagaccgcaa
      781 agtgaagaag ggaatcgaga agctgccgga cggcttcacg cacagtcccg tttttctgat
      841 ccgcaatttg ctgcgactgc tgccggccta ctacgtcgcc caaacgatta ctcgcgccga
      901 cgaacagagc gcatatatgc ctgaagagat tttctgccgt atcatggcgg cgtcgtatat
      961 cagtaaacga gatttgaaac cgacggcgtc gaccatgtcg cacgtggaag attttcagca
     1021 ctgttacctg aaactggtgg cggcgctcgg tggtccattc gacctggtgc tgaagacctt
     1081 gcaggagcga tcggccgtga tcaatcaccg ccatcgcatc accggggatg ccgtcacctg
     1141 gatcatcgaa gaagtgatcg ctatgaaaaa taagatcagg atcgacggtc tgcaggaagc
     1201 actcgacgcc tttatcgact cacaagtgct gatccctggc aaatggcatc ccgtcccggc
     1261 cgaacaactc aaatcaaaca ctctcaaatc gcaattgctg caaaagatcc acgaaaacct
     1321 cgaaatgtac aaagaatcga tttaatggaa atgatcaact gggctgaagt gcgcgcgaag
     1381 tttcccgtta cgagtgggag tgtctatctg aacacggctg cggctggacc actcgcagac
     1441 tcgactgcaa gagccggtga gctttattac gaacagatga tgaacgatgg cgacgtccat
     1501 tgggatgagt ggctcgcgcg ttgtgaggac attcgcaaac aggtcgccgc gttcatcaat
     1561 gctgagccgg aagagatcgg gttcacgacg aacacgtcga gtggaatgaa tctgatcgtt
     1621 gatgcactcg agcagcacgg cgaggtgatc tcgtgcgatc tggagtttcc ggtgacgacg
     1681 attccgtgga tgcatcgtcg cattccagtg cattttgtga agtcgattga tggcgaggtg
     1741 cgaccaaaag atgtgcgcgc ggcgatgaac acgcgcaccg ggatcatctc gatgagttac
     1801 gttcagttct caaatggctt tcgcagcgat cttgaagagc tcgcgagtat caagggcaat
     1861 cacgcgctcg tggttaacgc gagccaggcg gcgggcgcgt tcgagatcga cgtgaagcgg
     1921 atgaagatcg atgcgttatg ctcgacgggc cacaagtgga tgcgatcggg atacggttcg
     1981 ggatttgttt atatcagcaa agagctgcag gcggcttcgc ggcaacggat catcggttgg
     2041 ctgagtgttc acgatccata tggaatgaga aacgacgaga tacatttgcg ccatgacgtc
     2101 gcgtcgcgtg ctgagatcgg gtgtctccac tttgccggtg tgtttgcgtt gggagcatcg
     2161 atcgagttga tgcagtcgat cgggatcgag aacattcaag cgcgcgtgct cgagttgaat
     2221 cagaagttga cgtcgcgact tgatgagatt ggctggaagg tcctgtcgcc acttggggaa
     2281 gacaagtttc gctcagcgga aacgctcgtc gccgcggatg aaccggggaa aattgtcgct
     2341 caattggccc aacaaaaggt attagtgact gagaaaccgc agggctttcg tgtctccacc
     2401 gatctcttca acaacgaaga agatatagaa aaactagcac acggattaca cggattccaa
     2461 acggattatc agtgaaaatc cgtaatttat ctgtgtaatc tgtggcccgg ttttttggtt
     2521 ttcttagaag ccccagcaga attcgagtcg ttgaaattcc tgtgcgcgag agcgacgaac
     2581 ttctgcatga tcggtgagtc gtaaccgtta ctcaactgcg cgatgcccag ctcccagttg
     2641 atctgcgcgc cctcgatcca ccagcgtatc agtcgtccct tctcaacttc ctccttcacg
     2701 gactgcagcg gcacaatccc cacacccata tcctcttcaa ccatccgttt aatcgcctgc
     2761 tgtcgactca gttccattgc cacgcgcaac gacactccgg cctgcgcaaa aaactgatcg
     2821 atcaaacgcc gtgtattccc gccgcgttct ccgagaatca gtcgctcacc cgcaagcgtg
     2881 tatgcactga tcgtgcgctg cttcgcaagc ttgtgacgcg gcgacgcgat cgccaccagt
     2941 tgatcctcgc tcaaacgctc agtcttgatt ccacgtacgt ccaccggcag cgaaacaaac
     3001 gcaacatcga cttcacccgc aagaatctgg tccacgagca cttcgctggt ccccgatgtc
     3061 acggagatat ccgccgccgg atgttgttta cgcaactcct tgagaatcga cggcagttgc
     3121 tcagtcagga ccatcgccga cgcactgccg atccggagtc ggccgcgctc agccccggca
     3181 agctctgcga tttcatcgag cgcggcgtcg tgttcgcgga ggatgtggcg cgcgcgtcgc
     3241 agtagctgct cgcccgcttc agtcaggatc acatgccgcg gggcacgaac aaacaccttc
     3301 gccccgatct cagtctccaa ctgcctgatc tgcatgctaa tcgccgcctg ggtcacgtgt
     3361 acgcgtaaag ctccggccgt aaaagtaccc gattcagcga ttgctacgaa ggcgcgtaat
     3421 tgtcgaattt ccacaacagc aggctccccg atatcaagat ggattatcgt aataataaaa
     3481 tcttttagtt tccattataa atcagcgaac cctagaataa tcaacataat gttgaatgga
     3541 tcgttttctg gtccgcccga gccccaactg tgagcactga acgacgaaca gcagatctct
     3601 ctccaactag acgcgtgctc actcagaact cggtggtcgg tgtggacggc ggcgggacca
     3661 agaccgaagc cgtagtcatg gacgcgaacc agcgagtcat cggcgagggc cgcgccggac
     3721 cctcgaaccc attgcgagta ggaattgcag gagccgcagc cgccgtgcgc gaagcgatcg
     3781 atagcgcgtg tgcggccgcg aagctcaggc gtggcgatct gatcgctgcg caaatcggcc
     3841 tcgcgggagc gcgtcgtcgc gagttgcgag agcgcatgcg cgagacactg atgccgctcg
     3901 gtgtcggtga ggttgaagtg gttaccgatg ccactatcgc attgtatgga gcgactgacg
     3961 gcgcgccggg tctggttgtc atcgccggaa cgggatcgat ctgctgcggt atcaatgccc
     4021 gcggcaagcg tttctgcgcg ggcggctggg gaccgattgc tggtgatgaa ggcggcggcg
     4081 cgtggctggc tcgtcgcgct ctgcgcgcag tcgcttatgc ctttgatggg cgtggtccaa
     4141 agactgatct gaccgagctc gcctgctcgt atttccacgt caccagtcct gacgatctga
     4201 ccacagcgat ctatgcgccc acgatcacga atgagcgttt ggcaggcttt ggcagagacg
     4261 ttgtcgacgc agcgaagtca aacgatccag tcgcgcacca gatcgttgct gatggcggca
     4321 ccgagctcgg gcacgcggcg gtcgccgtga ttcgaaattt gcagatggag cgcgaacgtt
     4381 ttcagattgc gtacgttggt ggtgtgtttc gagcggcggg tgatttgatg ctcgacacgt
     4441 tgcgcgccga ggtgcgaaag gtcgcgccgc gtgcgtattt cgagccgcct cacttcgctc
     4501 cagccgtggc tgccgctaga atggcgcgtg accgcatcaa ccatgtcgct ctcgccgtct
     4561 gatatgaaaa cggaaaccgc gcttccaatc accgagcaag agaacccacg ctcaaaagat
     4621 ctcagttcac aatcgacagc gcagatcgtt gcgctcatga acgacgagga cgcgacggtt
     4681 gcagcggcag tgaagcgtgt gctcggcgac gttgttaaag cggttgatga gattgtcgtg
     4741 cggctagcga aaggcggacg actcttttat acgggcacgg gaacttcagg acgattgggt
     4801 gtgctcgacg cgtccgagtg tccgcccacg tttggtgtgt cgccagattt ggtccagggt
     4861 gtgattgccg gtggttacga tgcgtgccat cgtgcggtgg aggcttcgga agatgatgcg
     4921 gaggctggtg gttttgattt gaagtcacgt gggtttggga cgggggatgt gctcgtggga
     4981 atcgcggcgt cgggtcgcac tccatatact gtcggcgc
//
LOCUS       DASSFQ010000299         6523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_884499, whole genome shotgun sequence.
ACCESSION   DASSFQ010000299 DASSFQ010000000
VERSION     DASSFQ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884499"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2884
                     /locus_tag="VFH96_10745"
     CDS             167..2884
                     /locus_tag="VFH96_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET6974494.1"
                     /translation="MRIGKQVMCAEIIPFKPVTKSGWHALEPHSVSQLLDVNPQIGLT
                     AAQLTERQLRYGRNALQRIQSRSAWRVLSDQFASIVVALLAVAALISWLTGDGAEAIA
                     ILIVLALNAAVGFATEWQAGRALDALRQQSRTTARVRRDGFEITVDAEELVPGDVVIL
                     NAGDRVPADARLLEAVRLEAEESALTGESTTVEKTIDAVAAQTPLAERHSMLYLGTAI
                     AAGRAVAIVVSTGAATQLGKIGKLVAVSTKERSPLEIQLAHLGRRLVYLVLIIAALVM
                     LTGWWRGDGLWMMVEVGISLAVAAVPEGLPAVTTLILALGVLRMAQQRAIMRRLPAVE
                     TLGSTTVICADKTGTLTENRMTVREYYLSDGRQIDIGDLQNSLNGDELLEQAVRIGVL
                     CNEASFRSEVKDQTRSIGDPTETALLVIADALVLDVSHERAIHPKVAEQPFHTSTRRM
                     TTLHRKSDGQHFAALKGAPVVVLDACSHYRDAAGNTVPLDGEGLARFTTANAQMAGRA
                     MRVLGLAVKHFAADAKHSDEALESGYTFLGLVGMIDPPRPGVAEAIKRAKAAGIRTVM
                     LTGDQLNTGIAIARELGLGKTEPTALHARDLIDVDPKGLAELARNTDVFARVSPEEKL
                     RIVAALQHAGEVVAVTGDGVNDAPALKRANIGIAMGQRGTEVAKEAADVVLADDNFET
                     ILRAVKGGRTIYANITKFVHMMFSHNLGEVLMIFTAIAAGWPLPLLPLQILWMNLVTD
                     VFPALALAVEPASKDLMHQPPRSSRAGFISKPFLFLIGWQSLMLAAITLGVYAWALQV
                     YGPGSHSRTIALFALVSVQLGHTFNCRSRRRSAFAGVFRNPFIWIALVIVVLLQLLAV
                     YFAPLAAILGTVKPSSIDWLVIASCGLLAIGIVEIAKFIHRLRRLPHHV"
     gene            2896..4218
                     /locus_tag="VFH96_10750"
     CDS             2896..4218
                     /locus_tag="VFH96_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974495.1"
                     /translation="MSSILENTDISSAPADSSVFQSFWMGGFECSTHRLPRRKALGRF
                     AGQRLDLIESTRHGEFAREDYARLQEVGIRTARDGVRWHLIEESPYRYDFSSLIPMLR
                     AARETQTQVIWDLFHYGWPDGLNIWSGAFVERFARFARATAAVILDETGGPIYVTPAN
                     EISFVAWGGGDAGFLNPFAKQRGPELKAQLVRAAIAAIEAVREVDPRARICHAEPIIH
                     IAVDPDYPEEAPAAEGYRMSQFQAWDMLSGRFCPELGGRPEYLDLLGVNYYSNNQWIH
                     QDPHTPASRRRKDVLLPPSHPLHRPIRELLREVYERYRRPIFIAETGIEGDARPTWLR
                     YIGQEARAAAAAGVELEALCLYPIIDYPGWGDDRHCYSGLWDYADDAGRREIYEPLAQ
                     ELAHQQQLWTTQRRDHRAIEETVNIDKIDEAAHQADVATQKSRTKRRE"
     gene            4350..5024
                     /locus_tag="VFH96_10755"
     CDS             4350..5024
                     /locus_tag="VFH96_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6974496.1"
                     /translation="MRILVIEDDARMAGVIAKGLRENSYAVDVAQEGEAGLYQAAIND
                     YDVIVLDVLLPKHDGYEVCRELRTRGDTTPVLMLTARAAIDDRITGFDAGADDYLTKP
                     FSFRELLARIHALLRRDSQLRPDVLEIGDLVVDSISHRVSRASHEVQLTAKEYALLEY
                     LARRAGQLVSRAEIAAHVWDDTFDPFSNTIEVYMNRLRKKIDGKHSLKLLHTRRGEGY
                     MLDVRH"
     gene            5091..6404
                     /locus_tag="VFH96_10760"
     CDS             5091..6404
                     /locus_tag="VFH96_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6974497.1"
                     /translation="MTKRARPKVFPLAVLFALACLSTLHAQTQLSLDDALRLANAQAS
                     TYQTAAINERIAAEDVRQAQAAFLPKVTTPLSYIYTSPALGLPPGEPRTFSFVSANGI
                     GETVALVNVSGDFDIAGRLRASLARNRALLAAAHAGTEAAKRTLAQAVIEAYYGLALA
                     SAQRNAATGNLAAAEEFERATSLLLSGGEVASVDVTRAHLQTLTRRDELERARANEEV
                     AAGALRVFVGYEFSRPINATDLALDVPVDSDYQQFKAEEVSRRPEFIQLEQQLRAAQQ
                     EIRIAHADRLPSLSYSVNGGFDTDSLKSPRLKEHTGVSAAITFSIPIFDWGATRSRER
                     QAQLRVDLAENERTIALRGFTQQFYAARAQVDSAKRRIDVAREGVAKAEENVTASIAR
                     YRAGEAQIVEVTDAQTTLVEQRNALYQAIFDYQTALARLKQATGR"
BASE COUNT         1391 a         1880 c         1877 g         1375 t
ORIGIN      
        1 tgtcgctcgc ttcgcgagct tatgctccgg gctatgacaa cgacagatcc cggtcttatc
       61 gtttgttagg ccaacttcgt ggggaaagtt ccgcaagaag tgcgtggata atcacgcaag
      121 gcatgcgtcg gacgctcaat agccccgcat tcagcaggca cgggccttgc gaattggcaa
      181 gcaagtgatg tgcgcggaaa tcatcccttt taaaccggtt acaaaatcag gctggcatgc
      241 gctggagccg cactccgtct cacagctgct cgacgtcaat ccgcagattg gtctcaccgc
      301 cgcacaactt accgagcggc agcttcgcta cggtcgcaat gcccttcaaa ggattcaatc
      361 tcgttcagcg tggcgcgtac tcagcgatca attcgcaagc attgtggtcg cacttctcgc
      421 agtcgccgcc ttgatttcat ggctcacagg cgacggtgcc gaagcgatcg caattctgat
      481 tgttctcgct cttaacgccg ccgttggttt tgcgactgaa tggcaggcag ggcgcgccct
      541 tgacgcactg cggcaacagt cgcgcacgac cgcgcgagtg cgccgcgatg gttttgaaat
      601 aacggtcgat gcggaagagc tggtccctgg cgatgtggtg attctgaacg ccggcgatcg
      661 ggtgccggca gatgctcgtt tacttgaagc cgtccgtctc gaagcggaag agtcagcact
      721 gaccggtgaa agcacaacgg tggaaaaaac catcgacgca gttgcagcgc aaacacctct
      781 agctgaacgc cactcaatgc tttatctcgg gactgcgatt gccgctggtc gcgccgtcgc
      841 gatcgtcgtg agcaccggcg ctgccactca actcggcaag attggcaagc tggttgccgt
      901 ttcaaccaaa gaacgctcgc ctctcgaaat acagctcgcg catctcggac gtcgcctcgt
      961 ctacctcgtt ttgatcatcg ccgcgttggt gatgctgacc gggtggtggc gcggagatgg
     1021 tctgtggatg atggtcgaag tgggcatcag cctcgctgtg gcagccgtgc ctgaaggtct
     1081 tcccgctgtt acgactctga ttctcgcact cggcgtgctg cgcatggccc agcaacgcgc
     1141 catcatgcgc cgtctcccgg cggtagaaac tctcggcagc accaccgtca tctgcgctga
     1201 caaaaccgga actctgactg agaatcgaat gactgtccgg gagtattacc tttcggacgg
     1261 cagacagatc gacattgggg atctgcaaaa ctcactcaat ggcgacgagc tcctcgagca
     1321 agctgttcgt ataggagtgc tttgcaatga agcgtcgttc cggtcagaag taaaagatca
     1381 aactcgctcc atcggtgatc caacagaaac cgcgttgctc gtgatcgcag acgcgctggt
     1441 gcttgacgtt tcgcacgagc gggccattca tccgaaagtt gcggaacaac cgttccacac
     1501 atcaaccagg cgaatgacaa ctctgcatcg caagtctgac ggacagcact tcgctgcttt
     1561 gaaaggcgcg cccgtcgttg tactcgatgc ttgttcacat tatcgcgacg ctgctggtaa
     1621 caccgtgccc cttgatggag aaggcctcgc aagattcaca acggcgaatg cgcagatggc
     1681 aggccgcgcc atgcgagtgc tgggactggc agtaaaacat ttcgcagccg atgcgaaaca
     1741 ttctgacgaa gcactcgaga gcggctacac ctttctcgga ctcgtgggaa tgatcgatcc
     1801 tccgcgcccc ggtgtcgctg aagctatcaa gcgggcgaaa gcggcaggca tccgcaccgt
     1861 catgcttacc ggtgatcaac taaacaccgg catcgcgatc gcgcgcgagc ttggtctggg
     1921 aaagacggag cctactgctc tgcacgcgcg cgatctgatc gacgtcgacc cgaaaggttt
     1981 ggccgagctg gcccgaaaca ctgatgtctt tgcgcgcgtc tctccggaag aaaaattgcg
     2041 gatcgttgca gccttacaac acgccggtga agtcgtggcg gtcacgggcg acggcgtaaa
     2101 cgatgcaccg gcactgaagc gagcgaatat cggtatcgcg atgggccaac gcggcactga
     2161 agtcgcaaaa gaagctgcgg acgttgtact ggcggacgat aactttgaaa caatcttacg
     2221 agcagtcaaa ggtggccgca cgatttacgc caacatcacg aagtttgtgc acatgatgtt
     2281 ttcacacaat cttggtgaag tgctgatgat cttcacggcc attgccgcgg gctggcccct
     2341 gccgctcctg ccgcttcaga ttctttggat gaatctggtt acggatgtat ttcctgcgct
     2401 cgcgttggca gtagagccgg cgtcaaaaga cctcatgcat caaccgccgc gcagctcgcg
     2461 cgctggtttc atttcaaagc cgtttctgtt tttgattggc tggcagtcgt tgatgctcgc
     2521 ggcgatcact ctcggcgtgt atgcatgggc gctgcaggtg tatggtccag gatctcactc
     2581 gcgcaccatc gcgctcttcg cgctggtttc ggtccaactc ggtcacacct tcaattgccg
     2641 ctcgcgacgc cggtcagcgt tcgctggagt gtttagaaat cctttcatct ggatcgcgtt
     2701 ggtgattgtc gtgttgctgc aactcctcgc cgtctacttt gcaccgcttg cggcgatact
     2761 tggtacggtg aagccttcgt caattgattg gctcgtgatt gccagttgcg ggctactggc
     2821 gatcgggatt gtcgagatcg cgaagttcat ccacagatta cgcagattgc ctcaccatgt
     2881 gtaagattat tttccatgag cagcatttta gagaacacgg atatttcaag cgctccggca
     2941 gacagctcag tcttccagag cttttggatg ggcggattcg agtgctcgac gcatcgactg
     3001 ccccggcgta aagcgctcgg acgctttgcc ggacaacgac tcgaccttat cgaatcaaca
     3061 cgtcatggcg aatttgcccg cgaagattac gcgcgtttac aggaagtcgg catacgcacg
     3121 gcgcgcgatg gtgtgcgctg gcacctgatc gaggagtcgc cttatcgcta cgacttttcc
     3181 agtctcattc cgatgctgcg ggcggcgcgg gagacgcaaa cacaagtcat ctgggattta
     3241 ttccattacg gttggccgga cggactcaac atctggagcg gcgcgttcgt cgagcgcttc
     3301 gcacgcttcg ctcgcgccac cgctgcagtc attctcgacg aaacaggcgg accgatttac
     3361 gtcacgccgg caaatgagat ctcgtttgtg gcgtggggcg ggggcgatgc agggtttctg
     3421 aacccattcg ccaagcagcg cggacccgag ctcaaggcgc aactcgtgcg cgcggcgatc
     3481 gctgcaatcg aggccgtgcg cgaagtggat ccacgcgcgc gcatctgtca cgctgagccg
     3541 attattcata tcgcggttga tcccgactat cctgaagaag caccggcggc ggaaggttac
     3601 cggatgtcgc agtttcaggc ctgggacatg ctgagtggaa gattctgtcc ggagctcggc
     3661 ggtcgtcctg agtatctcga tctgctcggc gttaattact actcgaacaa ccagtggatt
     3721 catcaggatc cacacacgcc ggcctcgcgc cgtcgtaagg acgtgctgct gccgccctcg
     3781 cacccgctgc accggccgat tcgcgagctg ctgcgtgaag tttatgagcg atatcgtcga
     3841 ccgatcttca tcgctgagac cggcatcgag ggcgacgcgc gcccaacctg gttgcgctac
     3901 attggtcagg aggcacgcgc ggctgcggct gcaggtgttg agctcgaagc gttgtgtctt
     3961 tatccgatca ttgactatcc cggctggggt gatgaccgtc actgttacag cggtttgtgg
     4021 gactacgcgg acgacgcagg ccgtcgcgag atttacgagc ccctcgcaca agagctcgcg
     4081 caccagcagc aattgtggac cacccagcgt cgcgatcatc gtgcgatcga agaaacagtc
     4141 aacatcgaca agatcgacga agctgcacat caggcggacg tcgccacaca aaagagtcgc
     4201 acgaaacgca gagagtgagc cacaaaaagg cacaagagaa gagggccaca aaaagcacaa
     4261 aaagcacaaa atataattcc tcgacgtttt gtgctttttg tgccttttgt ggctaaactt
     4321 ctttcttgtg cttcttgtga tcaggattta tgcgaatact ggtgatcgaa gacgacgcac
     4381 gcatggcggg agtgatcgcc aagggattgc gtgaaaatag ctatgctgtc gatgtcgcac
     4441 aggagggtga agccgggctt tatcaggcgg cgatcaacga ctacgacgtc atcgtgctcg
     4501 acgtgttact gccgaagcat gacggatacg aagtttgccg cgagctgcga acgcgtggcg
     4561 acacaactcc ggtcctgatg ctcaccgcgc gcgccgcgat cgacgatcgc atcaccggtt
     4621 ttgacgcggg cgccgacgac tatctcacca aaccattttc atttcgcgag ttgctggcac
     4681 gtattcacgc actcctcaga cgcgactcgc aactccgtcc tgacgtgctc gagattggcg
     4741 acctcgtggt cgactccatc agtcaccgcg tttcacgggc cagtcacgag gtccaactga
     4801 ccgcgaaaga gtacgcgctg ctcgagtatc tcgcacgacg cgcgggccaa ctcgtttcac
     4861 gtgcggaaat cgcagcacac gtttgggacg acacgttcga tccgttttca aacacgatcg
     4921 aggtctacat gaatcgcctc cgcaaaaaga tcgacggcaa acactcgctc aaactgcttc
     4981 acacacgtcg cggcgaaggc tacatgctcg acgttcgcca ttaatttggc accatctatt
     5041 aatcttccat taaggttggt ccggctatac tcggccctta cgatacgagt atgacgaagc
     5101 gagcaagacc gaaggtattc ccccttgcgg tactattcgc gttagcctgc ctctcgacgc
     5161 tacacgcaca aactcagctt tcactcgatg acgcgttgcg actcgcaaac gcacaggcgt
     5221 cgacttatca aacagccgcc atcaacgaac gcatcgccgc cgaagacgtg cgacaagcgc
     5281 aagctgcatt tctgccgaag gttacaacgc cgctgagtta tatctacacg tcgccggcgt
     5341 tgggcctgcc gcccggcgag ccgcgtacat tcagttttgt ctctgcgaat ggcatcggtg
     5401 aaaccgtcgc gttagtgaac gtctccggag atttcgacat cgcgggccgt ttgcgtgcat
     5461 cactcgcccg gaatcgcgcg ttgctggccg ccgcacacgc aggcaccgaa gctgcaaaac
     5521 gcacgttggc ccaggctgtg atcgaggctt attacggact cgcactcgcc agtgcgcaac
     5581 gtaacgcagc gacaggaaat cttgccgccg cggaagagtt tgaacgcgct acatctttgc
     5641 tattgagcgg cggcgaagtg gcttcggttg atgtgacgcg cgcgcatttg caaacactga
     5701 ctcgtcgcga tgagttggaa cgcgcgcgcg caaacgaaga agtcgcagcc ggtgcgttgc
     5761 gggtctttgt cgggtatgaa ttctcgcggc ccatcaacgc gactgatttg gcgttggacg
     5821 ttcccgtaga ttctgattac cagcagttca aagcagaaga ggtttcgcgg cggcctgagt
     5881 tcattcagct cgagcaacag ctacgagcgg cgcaacagga gattcggatc gctcacgccg
     5941 atcgcttgcc ttccctgtcg tattcagtaa acggcggctt tgatactgat tcactcaagt
     6001 cgccgcggtt gaaggaacac accggagtgt cagcagcgat cacgttctcg attccgatct
     6061 tcgactgggg cgcgacgcgg agtcgtgagc ggcaggcgca gttgcgggtg gatctcgcgg
     6121 agaacgagcg gacgatcgct ttgcgcggtt tcacgcaaca gttttacgcg gcgcgggcgc
     6181 aagtggacag cgccaaacga cgcatcgacg tggcgcgcga aggcgttgca aaagcagagg
     6241 aaaacgtcac cgcgtcgatt gcccgttacc gcgccggcga agcacagatc gttgaagtca
     6301 ccgacgccca aacaaccctc gtcgagcaac gcaacgcgct ctaccaggca atcttcgatt
     6361 accaaaccgc gctcgcgcga ttgaaacagg caacggggcg atgagtgaca caggagcaat
     6421 tgtaggggtg gccctccgtg gccacccgtt gttggaatac acatcattcg ggaaccgggt
     6481 ggccacgtag ggccacccct acagttgatg caagcgatga acg
//
LOCUS       DASSFQ010000300        13061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2082554, whole genome shotgun sequence.
ACCESSION   DASSFQ010000300 DASSFQ010000000
VERSION     DASSFQ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13061
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082554"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..628)
                     /locus_tag="VFH96_10765"
     CDS             complement(5..628)
                     /locus_tag="VFH96_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974498.1"
                     /translation="MLGTFLALGGFWALKAQDPAPQQTPTTPTTQTEKQTMTKTAQLD
                     LSGTYAGTFANCEGLNGDTTLTITGDQFTTADGKSGRIIASKTKGYTAVALQMSGANS
                     PVMSFRGKKSGNKLTLSPVAGATSQCTFTTSKMAKTPTATGTEVANPSTTTPSTTPSQ
                     NPSPSPSPEASPEPNPSPSGSPRPRPNPSPTEPMPSPTPTPSPSPRI"
     gene            880..1884
                     /locus_tag="VFH96_10770"
     CDS             880..1884
                     /locus_tag="VFH96_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET6974499.1"
                     /translation="MQPENSIRLISVFVCTLVVTFLLTSCQPNNSGTNTATGGAGTKH
                     KLAFVTNNASDFWTIARKGTEKAAKDIPNLEVEFRIPSDGTAAEQQRVIDDLLAKGIQ
                     GIAISPVDPKNQTQMLNRAAGQTLVVTQDSDAPESNRVCYIGTDNVEAGRQAGQLVKE
                     ALPNGGKIMVFVGVLDAENARQRFQGLKEALAGSNVSIIDVRTDNTDLVRAKANAADT
                     LVNNPDIAGMVGLWAYNGPAILSAVKEAHKTDKVKIVAFDEQDETLSGIKDGSIYATV
                     VQQPFEFGYRSMELMSKYLNGDKSGVPASKQIFVPTKAIKKDNVEQFTKEINQLRGRS
                     "
     gene            1894..3411
                     /locus_tag="VFH96_10775"
     CDS             1894..3411
                     /locus_tag="VFH96_10775"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6974500.1"
                     /translation="MAANAPILEMEGISKRFPGVVALDQVSLTVGTGEIVALIGENGA
                     GKSTLMKILGGAISRDTGTIKINGQPVEIRSPREASALGIEFIHQELSVLDNLDVGAN
                     IFLRREPTTGGFLKLIDRKRVYRESDQILRKVGLDISSRTPLSQLSIAQQQLVEIARA
                     LSAGARIIIMDEPTSSLTLTETRRLLELCKDLKAENISLIYISHRMHEVEEIADRAVV
                     LRDGKNAGEIQRGEITHERMVRMMVGRDLKEFFKGAPVERQVGGDGGFEVRRLRTLRY
                     PNHQIAFNVAKGEVLGFAGLVGAGRSELARAIFGVEEALETDLSLNGQGLQIKGPQDA
                     ITHGIYLVPEDRRLSGLIVDFNVRENITLPNLEKYSSAKIIDRGKEMKAAREAVKAIN
                     IKTPTPEMRAANLSGGNQQKVVLAKWLTFAPRVLIFDEPTRGIDVGAKAEIYELIRKL
                     AAEGVSVIVISSEMEEVLGISDRIAVMHEGRLTGILDRAQFSEEAVMRLATGTDN"
     gene            3516..4505
                     /locus_tag="VFH96_10780"
     CDS             3516..4505
                     /locus_tag="VFH96_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974501.1"
                     /translation="MKKELGIFVLLIVLCIIVSVINPNFLLPINLQNLARQIGAFGIF
                     SIGLGLVIITGGIELSVGSMMALLGVLLSMMLTEWRFGVILAVLACMAIAMALTLGHG
                     LLITRLNMQPFIVTLCGLLFYRGLARFVTNDETKGFGTVEGLDFLRYLANGNLAGIPM
                     PFVLLLIIAAITWVALHRSVFGRYLFATGRNAEAARYAGINTKGIITLTYVIAGALTA
                     ISGMIFAFYTNSVSPANHGNAYELYGIAAAVLGGCSLRGGEGSVVGVLIGTALLQVLR
                     NLVNLLGIPSSLDFAVMGAVILIGVMADQIFTERRNRRQVAQMRQSTAVGEQS"
     gene            4502..5626
                     /locus_tag="VFH96_10785"
     CDS             4502..5626
                     /locus_tag="VFH96_10785"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HET6974502.1"
                     /translation="MKLQRKILALLLLMTCVAVAAAQSVTKESYGKTAAGENVDLYTL
                     RNSKGVEAKITNYGGILVSLKVPDRNGKFDDVVLGFNDLDSYLTKNDPYMGALIGRYG
                     NRIAKGRFTLNGVEYKLAVNNGENHLHGGIKGFDKVVWTGHEMKTPAGPAVVLTYLSK
                     DGEEGYPGNLNVRVVYTLTNNNELKIDYSFTTDKDTVTNLTHHSYFNLAGEGNGDILN
                     HVVTINANRFVPTDAGSIPTGKLKSVAGTPFDFLKATAIGARINQDDEQLKFGNGYDH
                     TWVINGRGMRLAATAYEATSGRVMQVWTTEPGVQFYTGNFLNGTLTGKSGKIYARRNG
                     FCFETQHYPDSPNQPSFPTTTLKKGATYKSTTIYRFSSKR"
     gene            complement(5692..7176)
                     /locus_tag="VFH96_10790"
     CDS             complement(5692..7176)
                     /locus_tag="VFH96_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974503.1"
                     /translation="MKATPTAPPKHLASAAARCIVAYRNMGSDLSHKEAPKAQKPELS
                     FSSLDPELRSGSRLKFEGRITLLSLAAGFPAVGLCAFLLWFDGYSAQVQWTVDLLLVL
                     LWLGISFNLKTRIVRPLQTLSNILGAIREGDYSIRGRRALSGDALGEVMLEVNDLGQT
                     LREQRLGAMEATALMRTVIAEIDVAVFAFDGEQRLQLVNREGETLLAQPSTRLLGRTA
                     EELGLSVCLNSSAGSAPQTMQMVFPGKVGRYDIRRSTFREGGQQHQLLVLTDLSQTLR
                     EEERIAWQRLLRVLGHELNNSLAPIKSVAGSLGDLMVREPEPPDWRDDMQRGLAVISS
                     RADSLARFVESYSKLARLPPPRLEPLKIGALVSRVARLETRLPVNVVSGPELVVQGDD
                     VQLEQLLINLVRNAVDASMETRGGVDVGWQQKNGQVEVWVRDEGPGLASTANLFVPFF
                     TTKSHGSGIGLVLSRQIAEAHGGTLTLENREQLKGCEARLRLPL"
     gene            complement(7085..8455)
                     /locus_tag="VFH96_10795"
     CDS             complement(7085..8455)
                     /locus_tag="VFH96_10795"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6974504.1"
                     /translation="MPRILIADDQADVLAALSLLLKGERYQIQSASSPEGVLRSIDTH
                     EFDVVLIDLNYARDTTSGSEGLDLLSRIRAADSNLPVVVMTAWGSVDLAVEAMRRGAR
                     DFIQKPWDNARLLAVLRTQVELAEALRRGRRLEAENMVLRDESSSELPQIVAESPAMK
                     SVLNLVERIGPADANVLITGENGTGKEVITRLLHSISSRRTKPLISLNAGALAEGVFE
                     SELFGHVRGAFTDAKADRVGRFELADGGTLFLDEIGNVPLNLQPKLLRVLETGDFERV
                     GSSKTQKVNVRLLSATNADLNAEVAQGRFRQDLLFRLNTVEIRLPPLRDRIEDIPVLG
                     EHFLKLHRERYRRAIMGFTPEALDALRQHLWPGNVRELDHVIERAVLMSPGQIVTAFD
                     LALEATPDARLSARLEEMSLEDAERLLIKKALARFEGNANRAAEALGLSRSALYRRLQ
                     KYGLGS"
     gene            complement(8501..10918)
                     /locus_tag="VFH96_10800"
     CDS             complement(8501..10918)
                     /locus_tag="VFH96_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974505.1"
                     /translation="MNDLRYALRSLIKQPGFAAVVVVTLALGIGASTAIFSVVNAVLL
                     RPFPFLHAERLQVVWGNYRTLNIEHLPAKAAEYEDYAKQTEVFDTVAAYANHSFNVTT
                     GAEPERIRGAYVSANLFPMLEAQPSAGRVFTSAEQRVVVLSDGYWQRRFAGDRGVINQ
                     TITLDNESYTVIGVMPAGFQFPHPSFSSGDPVDIWVPLSYAPDQVTKRSGPYFLNVLA
                     RLKPGVTLEQSRAHMNALAQRFERELSGYRGPNGEDGGWRITVTPLQEEVVGGSRRAL
                     LVLLFSVGLLLLIACANVANLLMMRSAKRHKELAIRAALGAGRWRIARQSCIEGVVLA
                     MMAAVLGLVFVNWGIDLLIALGPAIVPRTQEVNIDARVFAFMALTVAAISIGFGLIAA
                     RPVSKLDLNESLKNTRSPRRVKWSNVLVVAEVSLAVLLLTGSGLLVRSLWRLQQTNPG
                     ITSEELVSVEFDLPATTYSEAERASEFYRRLVTNVQALPGVQSVSFGTNQPLSGNAGS
                     DPFAIEGRKLDPSNLTAAGWQLVGADYLKTLGIQLVKGRDFTAEDMQQGAPPVAVINE
                     RMAARYWPNEDPIGRRITLGLPRPDNPWITIVGIARDVPHRAIDSKPEPDWYASRLVA
                     PQRHRFVFVRSALPSSTLTAAIRKTTTAIDPQQPLSSVKTMEQVIGTTTAPRRFNALL
                     LGVFAVIALVLATLGIYSVISYSITLRTQEIGIRMALGANRPAILMMVLRKGIVLTAI
                     GTAIGLVGALALTRWMSSLLFGVSATDPATYLIVLLVALGAALLACSIPARRATRVDP
                     LVALRYE"
     gene            complement(10981..>13061)
                     /locus_tag="VFH96_10805"
     CDS             complement(10981..>13061)
                     /locus_tag="VFH96_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974506.1"
                     /translation="LGRVFTNDEDKPGGTPVVVLSYALWQRRFGGQTSVLGQPITLNF
                     KPYTVIGVMPESYAYPSRVEMWVPVGQLSDQASWKMRGNHPGLYGVARLKSGTTFEQA
                     DADMNNVGANLEKQYPDSNTATRPRLRPLLEIFVSDVRRALWVIFGAVGFVLLIACAN
                     IANLLLARATARRKEMAIRTAVGASRWRLARQLLTESVLLSLTGGVLGLLLARWGVKL
                     ILYVSPDAIPRSREIGLDWTVLAFTLGVSFLTGILFGLIPAIQAGEVDVHETLKESGR
                     GNSGRQWLRSSLVVVEVATTLVLLIGAGLMIRSFYLLQKVNPGFSHEHLTSFSILLPQ
                     KKYATEESRQLFYNQMLENIRTLPGVESVAGASGLPLGNNGWQTSFVIDGQPPPPPEQ
                     TPLMEASLVTPDYFKAMNIPVLRGRVFNDRDDRSHLAGRDLSKLNETQRLLAGVNKIV
                     IDEEFAKKYWPNEDPVGKRVRLGRDADAEILEVLGVVGHVKMESLNQNTDRVQGYFSY
                     NQQPGSGMTVIIKAASDPNQLISSIRGVVKAIDADQPIYSPRTMAEIRAESVASERLN
                     LTLLSLFAGIALVLAIVGIYGVMSYSVTQRTHEIGIRMAIGARPLDVFKMIIGNGMKL
                     ALLGVAIGLAGAFVLTRFMATMLFGVAPTDTMTFGGISVLLITVALLACYLPGRRATK
                     VEPTISLRYE"
BASE COUNT         3018 a         3887 c         3507 g         2649 t
ORIGIN      
        1 ttaactatat ccgggggctg gggcttggag tcggagtcgg actcggcatt ggctcagtcg
       61 gcgacggatt cggtcttggc ctcggactgc cactcgggct cggattcggt tcgggactcg
      121 cctccggact cggagatggc gaaggattct gactcggcgt cgtgctcgga gtcgtggtcg
      181 acggattcgc tacttccgtt cccgtcgccg ttggcgtctt cgccatctta gacgttgtaa
      241 acgtacactg cgaagtcgct ccagcgaccg gcgagagcgt cagcttattg ccactcttct
      301 ttccgcgaaa cgacatcacc ggagaattcg ctcccgacat ttgcagagct acagcggtat
      361 atcctttcgt cttcgaggct atgatccgcc cactcttccc gtcggcagtc gtaaattggt
      421 ctccggtgat tgttagcgtc gtgtcaccgt taagaccttc gcaattagca aacgtcccgg
      481 cgtaggtgcc ggaaagatcg agctgagccg tctttgtcat tgtctgcttt tcggtctgtg
      541 ttgtgggcgt ggttggagtt tgttgtggcg ctggatcctg ggccttgagt gcccaaaacc
      601 cgccgagcgc caggaaagtt cccagcataa ctaacaagaa cattcttctg accatagtgt
      661 cctcccgtgt tcgttctggc gcacctgtgc ggcgcacttt cgacactcat gttgcagggg
      721 gtttcactac aagaaggagg ggacaccaat tttgtaccac tcagagcgtc aaatctcaac
      781 tcaatttcga ccacccggag ttttttagcg ctttctctct tggtttcttt gtggtaaaca
      841 tcttttgcgt acaacccaaa ctaaccgccg aggaagccaa tgcaaccaga aaactccatt
      901 cgattgatct ccgtcttcgt ttgcacgctc gtagttacat ttcttctgac aagctgccag
      961 cctaataatt ccggcaccaa caccgccacc ggcggtgccg gaacaaaaca caagcttgct
     1021 ttcgtgacga acaatgcttc cgacttttgg acgatcgcgc gcaaaggaac tgagaaagca
     1081 gccaaggata ttcccaatct cgaagtggag ttccgcattc cttccgacgg cacggcggcg
     1141 gagcagcagc gcgtgatcga cgacctgctc gcaaaaggca ttcagggtat cgccattagc
     1201 ccggttgacc cgaaaaacca aacacaaatg ttgaatcgtg cggctggtca aacactcgtc
     1261 gtgacgcagg acagcgacgc acccgaaagt aatcgcgttt gttacattgg cacagacaac
     1321 gtcgaggctg gccgtcaggc gggtcaactc gtcaaagaag cgctgccaaa cggcggcaag
     1381 atcatggtat tcgtcggcgt gctcgatgca gaaaacgccc ggcaacgttt tcagggtttg
     1441 aaggaagcgc tcgccggatc gaatgtctcg atcatcgacg tgcgcactga taacaccgat
     1501 ctcgtccgcg ccaaagcgaa tgccgccgac acgctcgtga ataatcctga tatcgcgggg
     1561 atggtcggac tttgggccta caacggacca gcgatcctct cagctgtaaa agaggcacac
     1621 aaaaccgata aggtaaagat tgtcgctttc gacgaacaag atgaaacatt aagcggaatc
     1681 aaagacggct cgatttacgc caccgttgtg caacaaccat ttgagtttgg ttaccgctcg
     1741 atggagttga tgtcgaaata cctcaatggt gataagtcgg gggttccggc cagtaaacag
     1801 atcttcgtgc cgacaaaagc tattaagaaa gacaacgtcg aacagtttac gaaagagatc
     1861 aaccagctcc ggggacgaag ttaagactag cctgtggcag caaacgcccc aatcctcgaa
     1921 atggagggaa tcagcaaacg attcccaggc gttgttgcgc tcgatcaggt gagtctgaca
     1981 gtcggcacgg gcgagattgt tgccctgatc ggcgaaaacg gcgccgggaa atcgacgttg
     2041 atgaagatcc tcggcggcgc gatctcgcgc gataccggca cgatcaagat caacggtcaa
     2101 cctgtcgaaa ttcgctcacc gcgcgaggcc agcgcgctcg gcatcgagtt tatccaccag
     2161 gagcttagtg tcctcgacaa tctcgacgtg ggcgcgaaca tttttctgcg tcgcgagccc
     2221 accacgggcg gcttcctgaa gctgatcgat cgcaaacgtg tttatcgcga gtcggatcaa
     2281 atcctgcgca aagttggtct cgacatctcg agccgtacgc cccttagtca actctcaatc
     2341 gcacaacaac aactggtcga gatcgcgcgg gcactgagcg ctggggcgcg catcatcatc
     2401 atggacgagc cgacgtcgag tttgacgctg accgaaacgc gacggctgct cgagctttgc
     2461 aaggatctga aagctgagaa catcagcctc atttacatct cgcatcgcat gcacgaagtc
     2521 gaagagatcg cggatcgcgc agtcgtgctg cgtgatggaa aaaacgcggg cgagattcaa
     2581 cgcggtgaga tcacgcacga gcgcatggtg cgaatgatgg tcgggcgcga tctcaaagag
     2641 tttttcaaag gcgcgccggt tgagcggcaa gttggaggcg atggtggttt tgaagtgcgt
     2701 cgcctgcgta ccttgcgtta tccgaatcac caaatcgcat tcaacgtcgc gaaaggcgaa
     2761 gtgcttggtt ttgcaggact cgtcggtgcg ggtcgttccg aacttgcaag ggcgatcttt
     2821 ggcgtcgagg aagcactcga aaccgatctt tcgctcaacg ggcagggact gcaaatcaag
     2881 ggtccgcagg atgcgatcac gcacggtatt tatctcgtgc cggaagatcg acgtttgtct
     2941 ggtctcatcg tcgacttcaa cgtgcgcgag aacatcaccc tcccgaacct cgagaaatac
     3001 tcgtcagcaa agatcatcga tcgcgggaaa gagatgaaag ccgcgcgcga ggcggttaag
     3061 gcgatcaaca tcaagacgcc gacgcccgag atgcgagcag caaatctcag cggtggcaac
     3121 cagcaaaaag tcgtgctggc gaagtggctt acgtttgcgc cgcgagtgct gattttcgac
     3181 gagccgactc gcgggatcga cgtcggtgcg aaagcggaga tctatgaact catccgcaag
     3241 ctcgcggcgg aaggcgtatc agtgattgtg atcagtagtg agatggaaga ggtgctgggc
     3301 attagcgacc gcatagcagt gatgcacgag ggacggctca ccggaattct cgatcgcgcg
     3361 cagttcagcg aagaggccgt catgcgtctc gccaccggca ctgataatta gccacaaaaa
     3421 ggcacaaaag cacaaaatga agaaaatgtt aggttccgta tttcattaac tattttgtgc
     3481 tttttgtgcc ttttgtggct taaacgaggg cttatatgaa aaaagagctt ggcatctttg
     3541 tcctgctaat cgttctctgc atcatcgtct cagttatcaa cccaaacttc ctgcttccga
     3601 tcaatcttca gaacctcgct cggcagatcg gcgcgtttgg aatttttagt atcgggctgg
     3661 gactggtgat catcaccggc ggcatcgagc tctccgtagg ctcgatgatg gcgctgctcg
     3721 gtgtgttgct ttcgatgatg ctgactgagt ggcgattcgg tgtgatctta gcagtgctcg
     3781 cgtgtatggc catcgcgatg gcactgacgc tggggcacgg tttgctgatc acacgtctga
     3841 acatgcaacc gttcatcgtc acgctttgcg gcctgctttt ttatcgcggc cttgcgcgct
     3901 tcgtcacgaa cgatgagacg aaaggcttcg gcacggttga aggtctggac ttccttcgct
     3961 atctcgccaa tggaaatctc gccggcatac cgatgccatt cgtcttgtta ttgatcatcg
     4021 ctgcaatcac atgggtggcc ctgcatcgct ccgtcttcgg gcgctacttg ttcgcgaccg
     4081 gacgcaacgc cgaagcagca cgttacgcgg gcatcaacac caagggcatc atcacgctaa
     4141 cttacgtgat cgccggcgcg ctcaccgcta tctccggaat gatcttcgct ttctatacaa
     4201 actccgtgtc gcccgccaat cacggtaatg cgtatgagct ttacggcatc gccgccgcgg
     4261 tgctcggcgg ctgttcgttg cgcggtggcg aaggatcggt ggttggcgtt ttgatcggca
     4321 ctgccctgct ccaggtgcta agaaatctcg taaacctact aggtattccc agctcgctcg
     4381 acttcgccgt gatgggcgcg gtgattttga tcggcgtgat ggcagatcag atctttactg
     4441 aacgacgtaa tcgacgacag gtcgcgcaga tgcgacagag caccgccgta ggagaacagt
     4501 catgaaactt cagcgaaaaa tccttgcatt gctcttgttg atgacgtgcg tcgcggttgc
     4561 tgcggcgcaa agtgtcacga aagagagtta cggcaaaacc gctgccggcg aaaacgtcga
     4621 tctctacacg ctccgaaaca gcaaaggcgt cgaagcaaag atcacgaact atggcggcat
     4681 cctcgtctcg ctcaaagttc ccgatcgcaa tggaaagttt gacgacgtcg tgctcgggtt
     4741 caacgatctc gacagttacc tgacgaaaaa tgatccatac atgggcgcgc tgattggccg
     4801 ttacggtaat cgcatcgcga aaggtcgctt cacgctgaat ggtgtcgagt acaagctcgc
     4861 ggtgaataat ggcgagaacc atttgcacgg cggtatcaaa ggcttcgaca aagtcgtctg
     4921 gaccggtcac gaaatgaaaa cgcccgcggg accagcagtt gtcttaacgt acctgagcaa
     4981 ggatggcgag gaaggttatc cgggaaatct gaatgtgaga gtcgtgtaca cgctcactaa
     5041 taacaacgag ctcaagatcg attactcctt tacaaccgac aaagacaccg tcacgaacct
     5101 gacgcaccac tcgtacttca accttgcggg tgaaggcaat ggcgacattc tgaatcacgt
     5161 cgtaacgatc aatgccaatc gttttgtgcc gactgacgcc ggatcgatac cgacaggcaa
     5221 attgaaaagc gtggcgggca cgccgttcga ttttctaaaa gccacagcga tcggtgcccg
     5281 catcaatcag gacgatgagc agttgaagtt tggtaatgga tacgatcaca cgtgggtgat
     5341 caatggccga ggaatgcgac tcgcggccac agcttacgaa gcaacgtcag gacgcgtcat
     5401 gcaagtatgg acgaccgaac ccggcgtgca gttttatacg ggaaactttt tgaatggaac
     5461 gctgactggt aaatccggga agatttatgc gaggcgaaat ggattctgtt tcgagacgca
     5521 gcactatccc gattcaccga accagccttc gttcccgaca acgacactca aaaaaggcgc
     5581 gacgtacaaa tccacaacga tctacaggtt ctccagtaag cggtagacag gatttacaag
     5641 attgagcagg atttacgtgt taatcctgaa aaatcctgta aatcctgtct actataacgg
     5701 taatcttaaa cgcgcctcgc atccttttag ttgttcccgg ttctcgagcg tcaacgtccc
     5761 accgtgcgct tcggcaatct gcctcgacaa aactaatcca atccccgatc cgtgcgactt
     5821 cgtcgtaaag aacggcacaa acaaattcgc ggtgctcgcg agcccggggc cctcatcacg
     5881 cacccacact tccacctgcc cgttcttctg ctgccacccg acatcgacac cgccacgcgt
     5941 ttccatcgac gcatcaaccg cattgcgcac gaggttgatc agcaactgct cgagctgcac
     6001 gtcatctccc tggaccacga gctcaggccc cgaaacaaca ttcaccggca atctcgtctc
     6061 cagccgcgcc acgcgcgaca cgagggcgcc gatcttcaac ggctcgagtc gcggtggcgg
     6121 cagtcgcgcc agcttcgaat acgactcaac gaaacgcgcg agcgaatctg cgcgcgaaga
     6181 gatcacagcg agcccgcgct gcatgtcgtc gcgccagtcg ggcggctcgg gttccctgac
     6241 catcaaatcg ccgagacttc ccgccaccga cttgatgggc gcgagcgagt tgttcagctc
     6301 gtggcccaac acgcgcaaca gtcgttgcca ggcaatgcgt tcctcttcac gaagcgtctg
     6361 gctcaggtca gtcaacacga gcaactgatg ctgctgcccg ccttcgcgaa acgtgctgcg
     6421 ccgaatatcg taacgcccga ctttacccgg aaacaccatc tgcatcgttt gcggcgcaga
     6481 gcccgcggat gagttgagac aaacggacag tccaagctct tcggcagtgc gtcctaacaa
     6541 tcgcgtcgag ggttgggcca ggagtgtctc accctcgcga ttgacaagct gcaaacgctg
     6601 ctcgccatcg aacgcaaata cggcaacatc gatctcggca atcaccgtac gcatcagcgc
     6661 ggtggcttcc attgcgccca atcgttgttc gcgcagtgtc tggcccaggt cgttgacttc
     6721 gagcatcact tcgccgagcg catcgcccga gagcgcgcgg cgtccgcgga tcgagtagtc
     6781 gccttcacga atcgcgccga ggatgtttga gagtgtttgg agcggacgaa cgatgcgcgt
     6841 cttcaggttg aaagagatgc cgagccacag caggacgagg agcaggtcga cggtccattg
     6901 cacctgagca gagtagccgt caaaccagag caggaacgca cacaaaccga cggctggaaa
     6961 cccggcggct agtgagagca gagtgatgcg cccttcgaat ttcaggcggc tgccgcttct
     7021 gagttcggga tcgagcgagg aaaacgacag ttctggcttt tgcgccttcg gtgcctcttt
     7081 gtggctaaga tccgagccca tatttctgta agcgacgata caacgcgctg cggctgaggc
     7141 caagtgcttc ggcggcgcgg ttggcgttgc cttcaaaccg cgcgagcgcc ttcttgatca
     7201 agagacgctc agcatcttcg agactcatct cttcgagacg cgccgacaga cgcgcatcag
     7261 gtgtcgcttc gagcgccaga tcgaacgcgg tcacgatctg gcccggactc atcagcaccg
     7321 cgcgctcgat cacgtggtcc aactcacgca cgttgcccgg ccacaggtgc tgtcgcaacg
     7381 catcgagcgc ctccggtgtg aaacccatga tcgcacgtcg ataacgctcg cggtgcagct
     7441 tcaggaaatg ctcccccagc accggaatgt cttcgatacg atctcgcaaa ggcggcaaac
     7501 gaatctcgac cgtgttcaga cgaaacagca ggtcctgacg aaaacgtccc tgcgcgactt
     7561 ctgcattcag atcggcattc gtcgccgaca acaaacgtac gttcaccttc tgcgtcttcg
     7621 acgatccgac gcgctcaaag tcacccgtct ctagtacgcg caacaacttc ggctgtagat
     7681 tcaacggcac gtttccaatc tcgtcgagaa agagtgtgcc gccgtccgca agctcgaagc
     7741 gcccgacacg atcggccttc gcatcagtaa acgcgccgcg cacgtgtcca aacaactcgc
     7801 tctcaaacac gccttccgcg agcgcgccgg cgttcaaaga gatgagcggc ttggtgcgtc
     7861 gtgacgagat cgagtgcagc agccgcgtga tcacttcctt gccggtgccg ttctcccccg
     7921 tgatcagaac gtttgcatca gccgggccga tacgctcgac cagattcagc accgacttca
     7981 tcgccggcga ctcagccacg atctgtggca actcgctcga cgactcgtcg cgcagcacca
     8041 tgttctcagc ttcgagacgc cggccacgac gtagggcttc agcaagctcg acctgcgtgc
     8101 gcaagacggc gagcagtcgc gcgttgtccc atggtttctg aatgaagtcg cgcgcgccgc
     8161 ggcgcattgc ttctaccgcg agatcgacgc tgccccacgc cgtcatgacg acaaccggaa
     8221 ggttcgaatc ggcggcgcga atacggctca gcaagtcaag accttcactg cccgacgtgg
     8281 tatcgcgagc gtagttaaga tcgatcagca ccacatcgaa ctcgtgcgta tcaatcgatc
     8341 tgagcacacc ctcgggcgac gacgcgctct gaatctggta acgctcaccc tttaatagaa
     8401 gactcaacgc cgcaaggacg tcagcctggt catcagcgat tagaatgcga ggcatagact
     8461 ggagataatg ccataggtcc tataggactt acaggtcata ttattcatat ctcagcgcta
     8521 ccagcggatc gacacgcgtc gctcggcgag cgggaatcga gcaggccagc aaggcagcgc
     8581 cgagcgcgac gagcagcacg atcaggtacg tcgcggggtc ggtcgcagag acgccaaaca
     8641 gtagcgacga catccaccgc gtcagtgcca gcgcgcctac caggccgatc gcggtgccga
     8701 tcgccgtgag cacaattccc tttcgcaaca ccatcatcag tatcgcgggc cggttcgcgc
     8761 cgagcgccat gcgaatgcca atctcctgcg tgcgcagcgt gatcgagtag gaaatgacgc
     8821 tgtagattcc gagcgttgcg agcaccagcg cgatcacggc aaacactccc aacaataacg
     8881 cattgaaccg gcgtggcgca gtcgtagtgc cgatcacctg ctccatcgtt ttcacactcg
     8941 acaacggctg ctgcggatcg atcgctgtgg tagtcttgcg gatcgcggca gtgagcgttg
     9001 acgaagggag tgcgctgcgc acaaacacaa accggtgacg ctgcggcgcg accaaacgtg
     9061 aagcatacca atcgggctcg ggtttggaat cgatcgctcg gtgcggcacg tcacgcgcga
     9121 tgccaacgat cgtgatccac ggattgtcag gacgcggcag accgagcgtg atgcgacgac
     9181 cgattggatc ctcgttgggc cagtagcgcg ccgccatccg ttcgttaatg actgctaccg
     9241 gcggcgcacc ctgctgcatg tcttcggcag tgaaatcgcg gcctttaaca agctgaatcc
     9301 cgagcgtctt taaatagtct gcgccgacaa gctgccagcc cgctgccgtc agatttgaag
     9361 gatcgagttt ccgtccttcg atggcgaacg gatcgcttcc cgcgtttcca ctcaacggct
     9421 ggttggttcc gaaactcaca gactgcacgc cgggcaatgc ctgcacattg gtaacaagac
     9481 gccggtagaa ctctgacgct cgctcagcct cgctgtaagt cgttgccggg agatcgaact
     9541 cgacactcac cagctcttca gaagtgatcc cgggattcgt ctgctgcaag cgccaaagac
     9601 tccgaaccag cagtcccgaa ccagtcaaca acaacaccgc gagcgagact tccgcaacca
     9661 ccaggacgtt gctccacttc acgcgacgcg gcgatcgagt gttcttcagc gattcattca
     9721 aatcgagctt cgaaaccggc cgtgcggcga tcagaccaaa gccaatgctg atcgctgcca
     9781 cagtcaaagc cataaacgca aacacacgcg catcgatgtt gacttcctgc gtacgcggca
     9841 cgatcgctgg tccaagcgcg atcagtagat cgatgcccca attaacaaac accagtccca
     9901 gaaccgccgc catcatcgcc agcaccacgc cctcgatgca cgactggcgc gcgatcctcc
     9961 aacggccggc accaagcgcc gcacggattg ccaattcctt gtggcgcttc gccgagcgca
    10021 tcatcaaaag attcgccaca ttcgcacacg caatcagcag taacaaccca accgaaaaca
    10081 gcaacacgag caacgcgcgt cgactgccac ccacaacctc ttcctgcaac ggcgtaactg
    10141 tgatgcgcca gccaccatcc tcgccgtttg ggccgcgata gccactcagc tcacgctcga
    10201 atcgttgcgc cagcgcgttc atgtgtgcac gcgattgttc gagtgtcacg cccggcttca
    10261 gccgcgccag cacgttgagg aagtacggac cactccgctt cgtgacttga tcgggcgcat
    10321 agctcaacgg cacccaaatg tctacgggat cacccgacga gaaactcgga tgcggaaact
    10381 gaaatcccgc cggcatcacg ccgatcacgg tataactctc gttatcaagc gtaatcgtct
    10441 gattgatcac gccgcggtca ccggcgaaac gccgctgcca gtacccatca ctcaacacaa
    10501 cgacgcgttg ctcagcgctc gtgaacacac gcccggctga aggctgtgcc tccaacatcg
    10561 gaaagagatt cgccgaaacg taagcgccgc gaatgcgctc gggctccgct ccggtcgtca
    10621 cgttgaaact gtgattggca taagccgcaa ccgtatcaaa gacttctgtt tgcttcgcgt
    10681 agtcttcgta ctcagcggcc ttggcgggca ggtgttcgat gttgagcgtg cggtaattac
    10741 cccaaaccac ctgcagccgc tctgcatgta aaaacggaaa cggccgcaac agcacggcat
    10801 tcacgacact aaagatcgcg gtactcgcac cgattcccag cgcgagcgtc accaccacga
    10861 ccgccgcaaa ccccggctgc ttaatcagac ttctgagagc gtaacgcaga tcgttcacgt
    10921 tttacttcca cagattacac agactacaca gagtttatct gtgtaatctg tggatggcat
    10981 ttattcatag cgcagcgaga tcgtcggctc cactttggtc gcccttcttc ccggaagata
    11041 acacgccagc aacgccaccg tgatcagcaa aaccgagatg ccgccaaacg tcatcgtatc
    11101 cgtcggcgcg actccaaaca acatcgtcgc cataaatcgc gtgagcacaa acgcgcccgc
    11161 cagtccgatc gccaccccaa gcagcgccag cttcatccca ttaccaatga tcatcttgaa
    11221 cacgtcgagc ggacgcgcgc caatcgccat gcgaatgccg atctcgtgcg tacgctgtgt
    11281 gactgagtaa gacatcactc cgtagatccc aacaatcgcg agcacgagcg cgatgcctgc
    11341 gaacaaactg agcagcgtca gattcaaccg ctcagacgcg accgactcag cccgaatctc
    11401 ggccatcgtg cgcgggctgt agatcggctg atccgcgtcg atcgccttca cgacgccacg
    11461 aatcgaggat attagttgat taggatcaga cgcggctttg atgatgacgg tcataccgct
    11521 ccccggttgc tgattgtaag agaagtaacc ctgcactcga tcagtgttct gattcaggct
    11581 ctccatcttc acatgaccaa ccacaccgag cacttcgagt atctccgcat cggcatcccg
    11641 tcccagcctc actcgtttcc caacaggatc ctcgttgggc cagtatttct tcgcgaactc
    11701 ctcgtcgatg acaatcttat tcacgccagc cagcaagcgc tgcgtctcat tgagcttact
    11761 gagatcgcgt ccggcgaggt gcgaacgatc gtctcgatcg ttaaatacac gcccgcgtaa
    11821 caccggaatg ttcatcgctt tgaagtaatc cggtgttacc agagaagctt ccatcagcgg
    11881 agtctgctcg ggtggcggtg gcggctgccc atcgatcaca aacgacgtct gccagccgtt
    11941 gtttcccaac ggtaaacccg aagctccggc gaccgattca actcccggca acgtacgaat
    12001 gttttcgagc atttgattgt agaacaactg gcgcgactcc tcagtcgcat acttcttctg
    12061 tggcagcaaa atgctaaagc tcgtgaggtg ttcgtgagaa aagcccggat taaccttctg
    12121 aagcaggtag aaactgcgaa tcatcagtcc ggcgccgatc agcaacacga gcgtagtcgc
    12181 aacttcaacc acgacgagcg agctgcgcag ccattgccgt ccactattac cgcgtccact
    12241 ctccttcaac gtctcgtgca cgtcgacttc gccagcctga atcgccggaa tcaacccgaa
    12301 aagaatgccg gtgagaaatg aaacaccaag cgtgaacgca agcaccgtcc aatcgagacc
    12361 aatctcacgc gagcgcggaa tcgcatccgg actgacgtac aaaatcaatt tcacgcccca
    12421 gcgcgccagc aacaatccga gcacgccgcc ggtcaacgac agcaaaacac tctccgtcaa
    12481 caactgtcgc gcgaggcgcc agcgactcgc accgacagcc gttcgaatcg ccatctcttt
    12541 ccgacgcgcg gttgcccgcg cgagcaggag attggcgata ttggcacacg cgatcagcaa
    12601 cacaaaccca accgcgccga agatcaccca cagcgctctt cgcacgtcgc tgacaaagat
    12661 ttccaatagt ggtcgcagcc ttggtcgagt agcggtattc gagtcgggat attgtttctc
    12721 gaggttggcg ccgacgttat tcatgtctgc gtctgcctgt tcgaacgttg ttcccgactt
    12781 gagacgcgcc acgccataca agccaggatg attaccgcgc atcttccagc tcgcctggtc
    12841 cgacagttgg cccaccggca cccacatctc gacgcggctc ggataggcat acgactcggg
    12901 catgacgccg atgacagtgt atggcttgaa attcaaagtg atcggctgac ccagcacact
    12961 cgtttggcca ccgaagcgac gttgccacag ggcgtaactc agaacgacaa caggcgtacc
    13021 gccgggcttg tcttcatcgt tcgtaaacac gcgaccgagt a
//
LOCUS       DASSFQ010000301        10083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2082612, whole genome shotgun sequence.
ACCESSION   DASSFQ010000301 DASSFQ010000000
VERSION     DASSFQ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082612"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFH96_10810"
     CDS             complement(<1..592)
                     /locus_tag="VFH96_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6974507.1"
                     /translation="MRDQVDQARWIAVLAVTAIALYLCWLMLRPFMGVLAWAGVLVVV
                     FYPVHKRIAERTGRRSLSALISCLLVVLVVVLPLTLVTAAVIHEFSKVAPNLPANLSE
                     VLSQQPAILGRVSTWAQDRFGIDVLTYQDFFIQQLRNVSQRLLGASFSLVGNIVTNIV
                     KAFFVVFTMYYLFRDGDKIANKLPDALPLNREQSEAL"
     gene            complement(892..1140)
                     /locus_tag="VFH96_10815"
     CDS             complement(892..1140)
                     /locus_tag="VFH96_10815"
                     /inference="COORDINATES: protein motif:HMM:NF039192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HET6974508.1"
                     /translation="MLTADLGRVMRFPPANNELEHAWIEAHREDYLNQWVALDRDRLI
                     AYGANAKQVYDEARKQGITSPYLERVLPKQEVFIGGWR"
     gene            complement(1146..1292)
                     /locus_tag="VFH96_10820"
     CDS             complement(1146..1292)
                     /locus_tag="VFH96_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974509.1"
                     /translation="MTSKQPAQKMDNQSGDALIQLLNECTIDTGVSDLAHQHDHYLYG
                     LTKT"
     gene            1394..2548
                     /locus_tag="VFH96_10825"
     CDS             1394..2548
                     /locus_tag="VFH96_10825"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HET6974510.1"
                     /translation="MRVTLFSVSLVLLLASEIVAQNQLTVTAVNKLKTARASETIELK
                     AEALAPLAEKDLMKIHVRDSAGKEVLAQAVDTDYDDYHKPDTLIFQADFGPNETKTFT
                     LTTGAKREYSKEDFRAYGRFVRERFDDFAWENDRIAHRTYGKALITWKGEPLTSSAID
                     IWSKRTTKLVINDWYMVDDYHADHGEGVDAYSAGPTRGCGGSGIWSNGQLYIPTNFVD
                     SRVLTNGPIRVMFELVYEPFDVNGVKVSQVLRVSLDAGSQLDRFQVSSQTAAGSEPLA
                     LAVGLKKVKEAQKEFNQARGWLTIWEPVEKKLGMQGLAVVVDPSAVDKLGEDKLNNLV
                     ILKPGVATPITYWAGFAWDRAGQITSAESWKAYVDQFAERLRSPIDITIK"
     gene            complement(2602..3474)
                     /locus_tag="VFH96_10830"
     CDS             complement(2602..3474)
                     /locus_tag="VFH96_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6974511.1"
                     /translation="MAKTFAATDDRPADAAAIRAHIESIFQAFIDGDEDKIFATHSED
                     WRGFLEGSRTPIKGIDDYMRANGIEWPRPANKPKSSPYFPAGTTYQVFDFDVHFYGPE
                     LAVANFFGEFQRKDGNTTITLRRFRIMDVYAKRKGSWIQVASHTVIDPEWRAEQMSRP
                     MNLTPQIRQRILAAREAVWKAWFSNDRAALERLIPEETIAIDNGQDGWSDRAAILAGA
                     KRFAESGGKLVKLEFPKTEIQVYGNTIIIYTTYLYETEVNGQRSTASGRATEMFVRRG
                     DEFVNVGWHMDSGK"
     gene            complement(3549..3740)
                     /locus_tag="VFH96_10835"
     CDS             complement(3549..3740)
                     /locus_tag="VFH96_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974512.1"
                     /translation="MTAKTAGTIQAADTIIHGRKRGNRMRPQYRWTSVGRNTVHVSEA
                     AARVFVVENQPQKAHKAQN"
     gene            3718..3972
                     /locus_tag="VFH96_10840"
     CDS             3718..3972
                     /locus_tag="VFH96_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974513.1"
                     /translation="MPAVFAVINRIAQARLQRWEPATLRELLFEFGDWLLYALLTPFV
                     FMISKEWPLTRPHLVRRAVLHFFFSLLFCVAWATCGQVFD"
     gene            3942..4787
                     /locus_tag="VFH96_10845"
     CDS             3942..4787
                     /locus_tag="VFH96_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6974514.1"
                     /translation="MGHLRAGVRLILIRIFAPEINLSWSGFGVEWLGWIFTTLPFGVA
                     VYLCVVGIEHATRYFIDAREREVQVARLSEQLSSARFAALQAQLNPHFLFNTLNTITV
                     LVRDNDRQGAVSIIEHLSELLRRTLTRHQADEVTLGEELDLVQQYVAIEQARFSDRLR
                     PEFRITEPLMSAAVPSFALQHLVENAIRHGIAKHTDAGLLLITATRSGDVLEISVIDD
                     GVGFDPEAPTPPAHGIDNTRERLRALYGERASLEITHRREGGTIATLKLPYRELRPEP
                     NGETS"
     gene            4774..5511
                     /locus_tag="VFH96_10850"
     CDS             4774..5511
                     /locus_tag="VFH96_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6974515.1"
                     /translation="MKQAKWKALIADDEPVARRGVRQLLASFPEFAVAGECRNGAEVL
                     AALNTSAPDVIFLDIQMPGVDGFEVIKRRTVEHMPAVVFLTAFDQFAIKAFETEALDY
                     LVKPVSEARFAMTIKRLVKRLESSSTREETIVVTTARGATVLHLNEIDWIEAAGNYSQ
                     LWVGTRSYFLRESLQLLEKRVQKNGFVRAHRRALVRLDGVRELTRTRAGASVAVLESG
                     VRVPISRRRSASFTAAFRATAGASPSS"
     gene            5713..6084
                     /locus_tag="VFH96_10855"
     CDS             5713..6084
                     /locus_tag="VFH96_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974516.1"
                     /translation="MAVSSRALVELVLLGPTNDRRQLQDSPVLGDVWVAYAVKPAAAI
                     DLLITPHKSAPAGPVAKLIADRIRALKMKRAKNEASKIAYLQGLVAAKLFFEELLKVI
                     VPMTEWWNDQKIQKQLQAYVN"
     gene            6376..7635
                     /locus_tag="VFH96_10860"
     CDS             6376..7635
                     /locus_tag="VFH96_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6974517.1"
                     /translation="MKYGSLSLRLGILLVLSAAFAGCVNKAQPSFERPPAPVVVSTAV
                     SQDVSNYMDALGKIVARETVAIQPQVSGRIEKIHFSDGANVRKGELLFTIDPRPFDAN
                     LKQAQANLAKDTAAKKQAEANLAREVARENWGRAQVERYRTLVEQGVVAREQYEQLRT
                     DYDTLKANSEAARAAVHSADETMKVDRAAIDTAKVQLSYCYIHSPIDGRAGQRIVDIG
                     NVVNPGGGISDSSSGIPSNALLVIERLDPIYADFTISQNNLSSVQEQMRAGTLRAEVR
                     LPDSPDDGVFGQLTFVDNSVQTETGQVTLRATLPNPGHRFWPGRFVNVRLMLGTITGA
                     VLVPANAAQMSAAGSYVYVVKPDSTAEQRTVSLGQRQGDLVVVQSGVAAGEKVVINGQ
                     LGVTPGGKVAIQQPPATPATAEAGGSK"
     gene            7632..>10083
                     /locus_tag="VFH96_10865"
     CDS             7632..>10083
                     /locus_tag="VFH96_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6974518.1"
                     /translation="MNLSEPFIRRPVMTAVLTLSVILFGVMAYFQLPVNDLPAVDYPV
                     IQVTVGYPGASPDTIANNIATPLERQFMQINGLEVVTSSSKQGVATFTLQFALEKNID
                     AAATDVQTAISQATGSLPSDLPSPPTYSKTNPNDQPIMYIAMTSNSVTPGQLYDYAST
                     QVGQRLSILPGVSQVNVYGTKSAIRIKADPSAMWARGISIDDLNNAIRQGTSYTGAGQ
                     LDSSSRTAVLRPQGQLETAEQYGNLIVGGSNNAPIYLRDVASVKDSVQDERVNMRFWV
                     RGRTVPTATVIVAVNRRAGANAVEVSKSIYEVLPMVQQELPGSISILPIYDRSASIVH
                     SVADVQATLIIAFVLVVIVIFAFLGRVTDTLIPVVALPLSLLITFIAMRGLGYSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRRIERGEPAFEAAINSAREISFTIMSMTISLAAV
                     FIPLVFMTGLVGRIFREFAITIVIAILASGVVSLTLTPLMCARLLKERGHGIRQTWME
                     RVIGGIERRVLALYGGSLWWFLRHRWISPVIWVICLAGTIWLFMLVPKTFLPPGDSGV
                     IFGAFIAQEGSSPIQMREYQDQVDKMLHNDPNVITDFTMTGATGFLASNQGITFTFIG
                     PADNRKPIAAVTAEMMGKLNTIPGVMTFLRPFPVLEISTGVTNQNQGQYAFTVSGVDP
                     NQVYETGQKLMAKLMTYPGFLSVSSDYYHNTPNLNIDIRRDQAKTYGISEARILTLLR
                     NAYSQNYTYLIKKPEDQYQVILEMEDSVREKPEDLSLLYIKSDDGKRLVPLSELVTWK
                     QTLGPQVVNHLNQFTSMSL"
BASE COUNT         2213 a         2802 c         2805 g         2263 t
ORIGIN      
        1 tgagtgcttc gctttgttcg cggtttagag ggagtgcgtc aggcagtttg ttggcgatct
       61 tgtcgccatc tcggaagagg taatacatcg tgaagacgac aaagaatgct ttgacgatgt
      121 tggtgacgat gtttcccacc aggctgaaag aagcgcccag caatcgttga ctgacattcc
      181 ggagctgctg gatgaagaag tcctggtatg tcagcacgtc gatgccaaaa cgatcctgcg
      241 cccaggttga gactctgccg agaattgcgg gctgctggct aagtacttct gaaaggttcg
      301 ccggcaggtt gggggcgact ttgctgaact cgtgaattac cgccgcggta acgagtgtga
      361 gcggtaaaac caccacgagc actaccagta aacatgaaat gagcgcgctc aaactacggc
      421 gcccggtgcg ttccgcaatt cttttgtgaa cgggataaaa aaccacgacc agcacgcccg
      481 cccacgccag cacacccata aacggtctga gcatgagcca gcacaggtaa agcgcaatgg
      541 ccgtcaccgc cagcacggca atccagcgcg cctggtcaac ctggtctctc atggtcctac
      601 cgtaaactaa acgagatgga gttgccagca gactggacag atcgtcaaga tacctagtgg
      661 gccagtttgg agaatacgcc gaaggcgttg gctagatgtc agcccagggt tggagcgcca
      721 gcgacaaccc tggggtagca attcagataa aatggtaaaa ccctgaaagg gtttggcggt
      781 tggcgaaccc tttcagggtt atgtcgctta ttaaattcct gacccagggt tgtcgcaagc
      841 tccaaccctg ggctgaaatt agcaaacgcc ttcggcgttc taactgaatc actagcgcca
      901 gcccccgatg aaaacctctt gttttggtaa aactcgttcg aggtaaggtg aggtgatacc
      961 ttgctttctc gcctcatcgt agacttgttt ggcattcgca ccgtatgcaa tcaaacgatc
     1021 tctatcaagc gcgacccact gatttaagta atcttcgcga tgagcttcaa tccaggcgtg
     1081 ctcaagttcg ttgtttgctg gcgggaatct catgactctt ccaagatctg ctgtaagcac
     1141 cttagttacg tcttcgtaag gccgtagaga taatgatcgt gctggtgggc gagatcggaa
     1201 acacccgtgt cgatagtgca ctcgttcaag agttgaatga gtgcatcccc ggattgatta
     1261 tccatttttt gtgcgggttg ctttgacgtc atcggacgtg ctccattcta gcatcctcgt
     1321 ttttgcttta acagtggcat cgatagtgta gaatcctcgg ccgttccatc atagtccggt
     1381 tgaggagtga ctgatgaggg ttactttgtt ttctgtttcg ctcgtccttc tgctggcgtc
     1441 cgaaattgtg gcgcagaatc aactcactgt taccgcggtg aataaactca aaacggcacg
     1501 cgctagtgag acgattgagc tgaaggcgga ggcgcttgcg ccgcttgccg aaaaggattt
     1561 gatgaagatt catgtgcgcg acagtgcagg gaaagaggtg ctggcccagg cagtcgatac
     1621 cgattatgac gattatcaca aacccgacac tctgatcttc caggctgatt ttggtccgaa
     1681 cgagacaaag acttttacgc taaccaccgg cgccaagcgc gagtactcga aggaagactt
     1741 tcgcgcctac ggccgtttcg tgcgcgagcg ttttgacgac ttcgcctggg agaacgatcg
     1801 catcgcgcat cgcacttacg gaaaagcatt gatcacatgg aaaggggaac cactcaccag
     1861 cagcgcgatc gatatctggt ccaagcgcac gacgaagtta gttatcaacg actggtacat
     1921 ggtcgacgac taccacgccg atcacggtga aggcgtcgat gcgtattctg ccggtcccac
     1981 ccgcggctgc ggcggcagtg gcatctggtc taacggccaa ctctacattc ccacgaactt
     2041 tgtcgactcg cgggtgctga ccaacgggcc gatccgtgtg atgtttgaac tggtttacga
     2101 accgttcgat gtgaacggcg taaaggtgtc gcaggtgttg cgcgtgtcgc tggatgcggg
     2161 cagccaattg gatcgctttc aagtctcgtc tcaaacggcg gcgggaagtg agccgctcgc
     2221 actcgcagtg ggtttgaaga aagtcaaaga agcacagaaa gagttcaatc aggcgcgcgg
     2281 ctggttaacc atctgggaac ccgtggaaaa gaaactcggc atgcagggtc tggccgtcgt
     2341 tgttgatccg agcgcagtcg ataagctcgg tgaagacaag ctgaacaacc tggtgatctt
     2401 gaagccgggc gtcgccacgc cgatcacata ttgggcgggc tttgcgtggg accgcgccgg
     2461 ccagatcaca tcagcggaga gttggaaagc gtacgtcgat cagtttgcag aaagactacg
     2521 atcaccaatc gacatcacta taaagtgagc cacaaaaggc acaaaaagca cataaaatga
     2581 ttttgtgcct tttgtggcta attatttgcc cgagtccatg tgccagccga cattcacgaa
     2641 ctcgtcgcca cgtcgcacaa acatctccgt ggcgcgacca ctcgcagtcg aacgttggcc
     2701 attgacttca gtctcgtata gatacgtcgt gtaaataatg atcgtgttgc cgtagacctg
     2761 aatctcggtc ttcggaaatt cgagtttcac cagtttccca ccactctcgg cgaatcgctt
     2821 cgcgcccgca aggatcgcgg cgcgatcgga ccacccatcc tgcccgttgt cgatcgcaat
     2881 cgtctcttcc ggaattagtc gctccaacgc cgcgcgatcg ttactaaacc acgccttcca
     2941 aactgcttca cgcgcggcga gtatgcgttg acgaatctgc ggcgttagat tcatcggccg
     3001 cgacatctgc tccgcacgcc actccggatc gatgacagta tgtgacgcga cctgaatcca
     3061 gcttcctttg cgtttcgcgt atacatccat gatgcggaag cgtcgcaacg tgatcgtcgt
     3121 gttgccgtct tttcgttgaa actcaccgaa gaagttggcg acggcgagtt ccgggccata
     3181 gaagtgcacg tcgaaatcga agacctggta agttgttccc gcaggaaagt aaggactcga
     3241 cttcggcttg ttcgcgggcc tgggccactc gataccgttc gcgcgcatgt aatcgtcaat
     3301 gcctttgatc ggcgtgcgag acccttctaa aaagccgcgc cagtcttcgg aatgggtcgc
     3361 aaagatcttg tcttcgtcgc catcgatgaa cgcctgaaaa atactttcga tgtgcgcacg
     3421 aatcgccgcc gcatcagccg gacgatcatc tgtagccgca aaagttttag ccacaaaaag
     3481 gcacaaaaag cacaaaataa gactgtgttt gaagagtggt tttagagaca ttcgaacttc
     3541 tcctctcatc agttttgtgc cttatgtgcc ttttgtggct ggttttcaac aacaaatacg
     3601 cgagcggcag cttcgctgac gtgaacggta ttgcggccca cgctggtcca gcgatactgt
     3661 ggccgcatgc gattgccacg cttgcgccca tggatgattg tgtcggcggc ctggattgtg
     3721 cccgccgttt tcgcagtcat caatcgcatc gcccaggcgc gtctgcaacg ctgggaaccg
     3781 gcgacgctgc gcgagttgtt gtttgagttc ggcgactggc tactctacgc gttgctcacg
     3841 ccgttcgtct ttatgatctc gaaagagtgg ccgttgacgc gcccacatct cgtgcgacgc
     3901 gcggtgctgc acttcttctt ttcactgctg ttctgcgtag cgtgggccac ctgcgggcag
     3961 gtgttcgact gatcctgatc aggatctttg cgccggagat taatctgtcg tggagtggat
     4021 ttggtgtcga atggctgggc tggatcttca cgacgctgcc gtttggcgtc gccgtgtatc
     4081 tctgcgtcgt cggcatcgag cacgcgactc gttacttcat cgacgcacgc gagcgtgaag
     4141 tccaggtcgc gcgtctctcc gaacaactct cgagcgcgcg ctttgccgca ctccaggcac
     4201 aactcaatcc tcactttctc ttcaacacac tcaacactat cacggtgctt gttcgggaca
     4261 acgatcgaca gggcgcggtc agcattatcg aacatctcag cgagctacta cgacgcacgc
     4321 taactcgtca ccaggcagac gaagtcacgc tcggtgaaga gctcgatctc gtgcagcagt
     4381 acgtggctat cgagcaggct cgtttctccg atcgcctgcg gcctgagttt aggattaccg
     4441 agccgttgat gtctgcggcg gttccgagtt tcgcgttgca acacttagtc gagaacgcga
     4501 tccgccacgg gattgcaaaa cacacagacg ccggtctcct gctgatcacc gccacacgat
     4561 ctggtgacgt gctggagatc tctgtcatcg atgacggcgt aggcttcgat cccgaagcac
     4621 ctacaccacc agctcatggc atcgacaaca cacgcgagcg tctgcgtgcg ctttacggtg
     4681 agcgagcgtc gctcgagatc actcatcgtc gcgaaggcgg tacaatcgcg acattaaaac
     4741 tgccgtatcg cgagctgcgt ccggagccaa acggtgaaac aagctaagtg gaaagcactg
     4801 atcgctgacg acgaacccgt ggcccggcgt ggcgtgcggc aactgctggc gagctttccc
     4861 gagtttgctg tcgccggcga gtgtcgcaac ggcgccgaag tgctggctgc attgaacacc
     4921 tctgcgcccg acgtgatctt tctcgacatt cagatgccgg gcgtcgatgg ctttgaagtg
     4981 atcaagcgac gaaccgtcga gcacatgccg gcggtggttt ttctcacggc gttcgatcag
     5041 tttgcgatca aggctttcga gactgaagca ctcgactatc tcgtcaagcc agtcagcgaa
     5101 gcgcgctttg cgatgacgat caagcggctt gtgaagcgtc tggaatcgag ctcaacacgt
     5161 gaagagacga tcgtcgtcac cactgcacgc ggcgcgacgg tgctgcactt gaacgagatc
     5221 gactggatcg aagcggccgg gaactactcg cagttgtggg ttggcacgcg cagttatttt
     5281 ctgcgcgagt cgctgcaact gctcgaaaag cgtgtgcaga agaatgggtt tgttcgtgcg
     5341 caccgccgcg ccctcgtgcg tctcgatggc gtgcgagagc tgacacgcac gcgtgccggt
     5401 gcgtcggttg cggtgcttga aagtggtgtg cgagtgccga tctcacgccg tcgcagcgcg
     5461 tcattcactg cggccttccg ggcaacagcg ggggcgagcc cctcttcctg acttattcgt
     5521 tttttttccc gcgctattga atagcacttc aagatgagag gcttgttcag ttgtttcacg
     5581 cgcccttgaa tagaacgggt caggaagagg ggcttgcccc cgctctaaaa ggattttctt
     5641 gttaatttcc tgatcctgta atttaattag cgcgccgttc gttttcttca catttccgta
     5701 agaggtttag tcatggctgt ttcttctcgc gctctcgtcg agctagtgct gcttggtcca
     5761 accaatgacc gcagacagct acaagactct ccggtgctcg gtgatgtgtg ggttgcctat
     5821 gcagtcaagc ccgccgcggc cattgatctg ctcatcacac cacacaagtc agctcccgct
     5881 ggtcctgtcg ccaagctcat cgccgatcga atccgagcgt tgaagatgaa gcgagccaaa
     5941 aacgaggcat cgaagatcgc gtatctgcaa ggcctcgtcg ccgcaaaact ctttttcgaa
     6001 gagttactga aagtgatcgt gccgatgacg gagtggtgga acgaccagaa gattcagaag
     6061 cagctccagg cttacgtgaa ttgacgagat agaagggcaa accctggtca ccaaagaaac
     6121 aaggcctggt cgccttcttc gctgaaaacc cgaagttcgc caaaatagtc tcaatcgtcg
     6181 acgaaaagaa accccacctg atcaacctga tcgataagat ctaatttagc cgcggatgaa
     6241 tacggatcaa atccgcggat tttttccgtg ttcatccgcg tcttcacaat ctcttaacaa
     6301 ctttttacct gcatttaaca tacttctgcc gaaccctgtt tagaattctc ctcgtataat
     6361 ggcacaatga accgcatgaa atacggaagt ttgtcccttc gtctgggaat tttactggtg
     6421 ctcagtgctg cgttcgctgg ttgtgtgaat aaggcgcagc ccagttttga gcgcccgcct
     6481 gcgcccgtcg ttgttagtac ggctgtgtcg caggacgtgt cgaactacat ggatgcgctc
     6541 ggtaagattg tcgcgcgtga aacggtggcg attcagccac aagtctccgg ccgtatcgag
     6601 aaaattcatt tcagtgacgg cgcaaacgtt cgcaagggcg agttgctttt cacgatcgat
     6661 ccgcgtccgt ttgacgcaaa cctgaaacag gcacaggcca atctcgcgaa agacacggcc
     6721 gccaagaaac aagcggaagc gaacctcgcg cgtgaagtcg cgcgtgagaa ttggggccgc
     6781 gcacaggttg aacgttatcg aacgctcgtc gagcagggcg tcgttgcgcg cgagcagtac
     6841 gaacagctcc gcaccgacta cgacacgctc aaagctaatt ccgaagccgc gcgcgccgca
     6901 gtgcacagcg ccgacgagac gatgaaagta gatcgcgcag cgatcgacac agcgaaggtc
     6961 caactcagtt actgctacat ccattcgcca atcgatggcc gcgccgggca gcgtatcgtc
     7021 gacatcggca acgtcgtgaa tcccggcggt ggaatctcag actcgagctc aggaatccca
     7081 agtaacgctc tacttgtgat cgagcgactc gatccgatct acgctgattt cacgatctcg
     7141 caaaacaatc tttcatcagt gcaggaacaa atgcgcgccg gcacgctcag ggctgaagtg
     7201 cgactgccgg attcacccga cgacggagtg tttggacaac tgacgtttgt cgataactca
     7261 gtgcagaccg aaaccggtca ggtaacgttg cgcgcgacgc ttccgaatcc cggacatcgc
     7321 ttctggcccg gtcgcttcgt caatgttcga ctcatgctgg gcacgattac gggagcagtg
     7381 ctcgtgcctg caaacgcggc acagatgtca gccgcgggct cgtacgttta cgtcgtcaag
     7441 cctgattcga ctgccgagca gcgcacagtt tcgctgggtc agcgacaagg cgatctcgtt
     7501 gtggtccaaa gcggcgtcgc agcgggtgaa aaggtggtta tcaacggaca actcggtgtc
     7561 acacccggag gtaaagtcgc aatccagcaa cctccggcca ctccggccac agcggaagcg
     7621 ggtggatcga aatgaatctc tctgaaccat tcatccggcg gccagtaatg accgcggtct
     7681 taacgctttc cgtaatcctt ttcggggtga tggcctactt ccaactcccc gtgaacgatc
     7741 tgccggcggt cgactatcca gtgatccagg tgacggtcgg ctatcccggc gcgagccccg
     7801 acacgatcgc aaacaatatc gcgacaccac tcgagcgcca gttcatgcag atcaatggac
     7861 tggaagttgt gacctcgtcg agcaaacagg gtgtggccac gttcacgctc cagtttgcac
     7921 tcgaaaagaa tatcgacgcc gctgcgactg acgttcagac cgcgatctcg caagcgaccg
     7981 gcagtctccc gtccgatctg ccgtcaccgc cgacttactc gaagacgaac ccgaacgatc
     8041 agccgatcat gtacatcgcg atgaccagca attcggtcac gcccggtcaa ctttacgact
     8101 acgcgagcac gcaggtgggc cagcgtctca gcattctgcc gggcgtgtcg caggtcaacg
     8161 tttacggcac aaaatcggcg attcgcatca aggcggatcc gtcagcgatg tgggcgcgcg
     8221 ggatctcgat cgacgatctg aacaacgcaa tcaggcaggg aacgagttac acgggcgcgg
     8281 gccagttgga tagttcttcg cgcaccgcgg tcctgcgtcc gcaaggccaa ctcgaaaccg
     8341 ccgaacaata tggaaatctg atcgtcggtg gctcgaacaa cgcaccgatc tatcttcgcg
     8401 atgtcgccag cgttaaagac tcggtccagg acgagcgcgt caacatgcgc ttttgggtgc
     8461 gtggccgaac tgttccgact gcgactgtga tcgtggctgt gaatcgacgc gccggcgcca
     8521 acgcagttga agtttcgaag tcgatttacg aagtcctgcc gatggtccag caggagctgc
     8581 cgggctcgat cagcatcctg ccgatctacg atcgctcggc cagcatcgtg cactccgtcg
     8641 ccgacgttca ggcaacactc atcatcgcgt tcgtgctggt cgtgatcgtg atctttgctt
     8701 ttctcggcag ggtgactgac acgctcattc ccgtcgtcgc gctgcccctc tcgttgctga
     8761 tcacgttcat cgcgatgcgc ggtcttggtt acagtctcga caatctttcg ttgatggcgc
     8821 tgacgcttgc gattggtttt ctggttgacg acgcgatcgt gtttctcgaa aacacagtgc
     8881 gacggatcga gcgtggtgaa ccggcattcg aagcggccat caacagcgcg agagaaatca
     8941 gcttcacgat catgtcgatg acgatctcgc tcgcggctgt gttcatcccg ctcgtgttca
     9001 tgaccggtct cgtcggccgc atctttcgcg agtttgcgat cacgatcgtc atcgcgattc
     9061 tcgcctccgg cgtcgtgtca ctgacgctta cgcccttgat gtgcgcgcgc ttgctgaaag
     9121 agcgtggcca cggcattcga cagacgtgga tggagcgcgt gattggcggc atcgagagaa
     9181 gagttttagc tttgtatggc ggttcactct ggtggtttct gcgtcatcgc tggatctcgc
     9241 cggtgatctg ggtgatctgt ttggcaggca cgatctggtt gttcatgctc gtgccgaaga
     9301 cgttcctgcc gccgggagac agcggtgtga tcttcggcgc attcatcgcg caggaaggct
     9361 cgtcgccgat acagatgcgc gagtaccagg atcaggtcga caagatgctg cacaacgatc
     9421 cgaacgtcat cactgacttc acgatgacgg gcgcgaccgg attcctcgcc tcgaaccagg
     9481 gcatcacgtt tacgttcatt ggaccagcgg acaaccggaa accgattgca gcagtgactg
     9541 ccgagatgat gggcaaactc aacacgattc ccggtgtcat gactttcctg cgaccgtttc
     9601 cggtgctgga gatcagcacc ggcgtcacca accagaacca gggccagtat gcgtttactg
     9661 tctcgggcgt tgatccgaac caggtttacg aaacgggcca aaagctcatg gcgaagttga
     9721 tgacgtatcc gggctttttg agcgtctcgt cggattacta tcacaacacg cccaacctga
     9781 atattgacat tcgtcgcgat caggcgaaga cttacggcat ttcggaagca cgtatcctga
     9841 cgttgctgcg caatgcttat tcgcaaaact acacttacct gatcaagaaa cccgaggatc
     9901 aataccaggt gattctcgag atggaagact ccgtgcgcga gaagcctgaa gatctgtcgc
     9961 tgctgtacat caagtcagac gatggtaaaa gactcgtgcc gctgagcgag ctcgtcacgt
    10021 ggaaacagac gcttggccca caagtggtga accacttgaa ccagttcacc agcatgtcgc
    10081 tct
//
LOCUS       DASSFQ010000302         6004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1033380, whole genome shotgun sequence.
ACCESSION   DASSFQ010000302 DASSFQ010000000
VERSION     DASSFQ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033380"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1970
                     /locus_tag="VFH96_10870"
     CDS             171..1970
                     /locus_tag="VFH96_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974519.1"
                     /translation="MSKKLKLSHLLRPHRGMLALAFIAVVGEAVTDLLEPWPLKIVFD
                     YVFGTKHMPAWMSGLIGGTFGLNKVAILHFAVLAVIVIAVGGALSSYAEKYLTTSVGS
                     WVMHDLRGVLYHHIQRLSLAYHDHKSTGDLISTVTADIDSIQDFISQALLGILVSVLT
                     LVGMLAVMFYLNWRFTLIALSITPFLFLVVYTYTHRIKQASRALRKKEGEVVSVVAEV
                     LGSIRVVKAFAREDYEEARLAQQSLENVEIALRARSLKAKLAPVVQVIVAVGTCLVLW
                     YGVNLVLTDQMTSGSLLVFLLYLGKMYKPMRDLSKMTDTMSKAAVGYERIRAVIETEN
                     EVRNLPGAKKAPAFKGAIEFEHVYFGYDKGYRTLEDVNLRVEPGQLAALVGPTGAGKT
                     TITGLIGRFYDPEKGIVKIDGEDIRNYKMKSVRRQMSFVLQETLLFRAPVWQNIAYGK
                     PEATREEILRAAELANADEFIKNMPQQYDTMVGEKGVTLSGGQRQRIAIARAIIRNSP
                     ILILDEPTSGLDAASEELVFEALTRLMEGRTTIVIAHRLATIRRADVIFVIDHGTVVE
                     RGTHDALLKEGGLYSHLYEIQFRNKSEPQITQI"
     gene            complement(2007..3473)
                     /locus_tag="VFH96_10875"
     CDS             complement(2007..3473)
                     /locus_tag="VFH96_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET6974520.1"
                     /translation="MSIHFKILGVTLIAMFLLKGCDKAPKYAKPAVATPAAYKEIAPD
                     AFKETDDWKFAHPSDAVIRGKWWEMFNDPQLNSLEEQLNTANQNIALADANFRAARAL
                     VKESRSEYFPTVTTSPSIIVSRQSAAATHSNVSPGRMNTNYTLPFDASWEPDFWGRIR
                     NTVVASTSEAQATAADLQNVRLSVEAELAFDYYQLRSVDAEKALLDSTIAAFQEGLDL
                     TRVRFETGIVSDEDVAQSETQLKTTQAQATDLGIQRAQLEHAIALLTGQPASTFSLPV
                     APLDTKPPEVPVGLPSQLLERRPDIAAAERRVAEANAEIGVTKAAFFPSLVLSATGGL
                     ESTSIASWFSWPARFFSLGPTLSQTIFDKGRRKAQTEFARAQYDATAATYRNTVLTSF
                     QEVEDNLATLRILSRELEQQNEAVASAQRALTLAMERYKSGIDSYLNVITAQATLLNN
                     QRTAVNLQAQQMTASVELIKALGGGWNASQLPTPKQLK"
     gene            complement(3499..>6004)
                     /locus_tag="VFH96_10880"
     CDS             complement(3499..>6004)
                     /locus_tag="VFH96_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6974521.1"
                     /translation="GLEDVRNMLSQQNANLAKGQLANNKTTSDIAVNDQLLQAAGYRP
                     LIVGYSNGAAIRLSDIANVQDSVENLRSAGLANGKPCALVIIFRQPGANIIDTVDSVR
                     AALPQLKASIQSAIDLTVVMDQTTTIRASVRDVERTLIISIGLVIMVVFVFLRNPRAT
                     IIPAVVVPVSLIGTFGVMYLCHYSLDNLSLMALTISTGFVVDDAIVVIENVTRHLEQG
                     MRPLQAALLGAKEIGFTVLSISISLVAVFTPLLLMGGIVGRLFREFAVTLSVAILVSL
                     VVSLTTTPMMCSRLLKHNEEHGRLYRASERVFQWILSLYERSLTWVLRHPLPMLLVLL
                     ITIGVNAYLLWIVPKGFFPQQDTGRLAGGIQGAQDSSFTAMRQRMARFVDIIKSDPAV
                     ANVVAFTGSGGGTTTNTGRMFISLKPLDERKVSADEIIRRLRRQLATVEGASVFLQAT
                     QDLRVGGRGGNAQYQYTIQSENLDDLVKWAPILMQELRKVSIVTDVNSDQQNSGLQAS
                     LDYDRATAQRLGITPQDIDSTLYDAFGQRQVSTIFRQLNQYHVVMEVEPRFWQSPEGL
                     KEIYLHAAAGGVVPLSAVAHYAPATAPLAVNHQGQFPAVTFSFNLAPGVALSDAVQAI
                     NQTEQSIGMPATIHGSFAGTAQAFQTSLASEPLLIVAALLAVYIVLGILYESYIHPIT
                     ILSTLPSAGVGAVLALLVTHMDLSLIALIGIILLIGIVKKNAIMMIDFALAAERGEGK
                     SSRDAIFQACMLRFRPIMMTTMAALLGALPLALGTGTGAELRRPLGISIIGGLLVSQM
                     LTLFTTPVVYLYLDRIKPRMTRITILDQNRSESLSV"
BASE COUNT         1315 a         1734 c         1640 g         1315 t
ORIGIN      
        1 ctccagtcaa agccacttca caacggcgtt ccgcaggctg gccggggcca cgcctaaagc
       61 attccgcgcc gcgctctgat ctcgagggga atgttagaca ggattacagg attttcagga
      121 tttacaagat aatcatgtga atcctgtaaa tcctgaaatc cggtctacca atgtctaaga
      181 aactaaaact cagtcatctg ctacgacctc accgcggcat gcttgcgctt gcgttcatcg
      241 ccgtagtcgg cgaggccgtt accgatctgc tcgaaccctg gccgctaaaa atcgtcttcg
      301 actacgtgtt cggaacgaag cacatgccgg cgtggatgtc gggattgatt ggcggcacgt
      361 ttggtctaaa taaagtcgca attcttcact tcgccgtcct cgccgtaatc gtcatcgcag
      421 tcggtggtgc tctcagctct tacgccgaaa agtatctgac cacttcggtc ggttcatggg
      481 tgatgcacga tctgcgcggc gttctctatc atcacattca acgcctttct ctcgcttatc
      541 acgatcacaa gtccactggc gatctgatca gcacggtcac cgccgacatc gattccattc
      601 aggatttcat ctcgcaggcg ctgctcggca tcctcgtgag tgtgctgacc ctcgtcggca
      661 tgctcgcggt gatgttttat ctcaactggc gcttcactct gatcgcgctt tcaatcacgc
      721 ctttcctgtt tttagttgtc tacacataca cgcatcgcat caagcaagca tcacgcgctt
      781 tgcggaagaa ggaaggcgaa gtagtttcgg tcgtcgcgga agtgttgggt tcgattcgcg
      841 tcgtgaaagc gtttgcgcgc gaggattacg aagaagcacg actggcgcag caaagtctag
      901 agaatgtcga gatcgcgctg cgtgcacgca gcctcaaagc gaagctggcg ccggtggtcc
      961 aggtgatcgt cgcggtggga acgtgcctgg tgctctggta cggtgtcaac ctcgtgctga
     1021 ccgaccagat gacttccggc tcgctgcttg tgttccttct ttacctcggc aagatgtaca
     1081 agccgatgcg cgacctctcg aagatgactg acacgatgtc gaaagcggcc gtcggctacg
     1141 agcgtatacg cgcggtgatt gagactgaga acgaagtacg caatctgccg ggcgcaaaga
     1201 aagctccagc cttcaaaggc gcgatcgaat tcgagcacgt gtatttcggt tatgacaaag
     1261 gttatcgcac gctcgaggac gtcaatctgc gcgtcgagcc gggtcagctt gcagcgcttg
     1321 tcgggccgac gggtgcggga aagacaacca tcaccggctt gattggccgg ttctacgatc
     1381 cggaaaaagg aatcgtgaag atcgatggtg aggacatcag gaattacaaa atgaaatcgg
     1441 tccgacggca gatgagtttt gtcttgcagg agacgctgct cttccgcgcg cctgtttggc
     1501 aaaacatcgc gtacggcaaa ccggaagcga cacgcgagga aatcctgcgc gccgcggagc
     1561 tggcgaacgc tgatgagttc atcaaaaaca tgccgcagca gtacgacacg atggttggcg
     1621 agaagggcgt cacgttgtct ggtggtcagc gccaacgtat cgccatcgcg cgcgcgatca
     1681 ttcgcaactc acccatcctg atacttgatg agccgacgtc tggtcttgat gcggcttcgg
     1741 aggagttggt attcgaagca ctgacaagat tgatggaagg tagaacaacg atcgtgattg
     1801 cgcatcgact ggcaaccatc cggcgcgccg acgtgatctt cgtcatcgat cacggcacgg
     1861 tcgtcgagcg cggcacacac gacgcattgc tcaaagaagg cgggctttat tcacacctct
     1921 acgagatcca atttcggaat aaatccgagc cacagattac acagatttaa atccggattt
     1981 ttatccgtgt aatctgcgga taatactcac ttgagctgtt tgggcgtcgg tagctgggac
     2041 gcgttccaac caccaccgag tgctttgatc aactcaacac tagcggtcat ctgctgcgct
     2101 tgcaggttca ctgcggtacg ttgattgttc aagagcgtcg cctgcgcggt gatcacgttc
     2161 agataactat caatcccgga tttgtagcgc tccatagcca gcgtcagcgc ccgctgggcc
     2221 gacgcgaccg cctcattctg ctgctccaat tcacgcgaga gaattcgcag cgtcgccagg
     2281 ttatcttcca cttcctgaaa cgacgtcaac accgtattcc ggtaggtcgc cgccgtcgca
     2341 tcatattgcg cgcgcgcgaa ctcggtctgc gctttacgtc ttcctttatc aaaaatcgtt
     2401 tgactcaaag ttggcccaag cgaaaagaaa cgcgccggcc acgagaacca gctcgcgatt
     2461 gacgtgctct cgagtcctcc agtcgcgctg agcacgagcg atggaaagaa cgccgccttc
     2521 gtgactccaa tctccgcatt cgcctcggcg acacgtcgtt cagccgcagc gatatccggt
     2581 cgacgctcga gcagttgcga aggcaaacca actggaacct caggcggctt cgtatctaat
     2641 ggcgcaaccg gtaacgagaa cgttgacgct ggctgaccgg taagcaatgc gatcgcgtgt
     2701 tcgagctgtg ctcgttgaat ccccagatcg gtggcttgcg cctgtgttgt tttcagctga
     2761 gtttctgact gggccacgtc ttcgtccgac acgataccgg tttcaaagcg aacacgcgtc
     2821 agatcgagcc cttcttgaaa cgcggcgatc gtcgagtcga gcaaagcttt ttccgcatcg
     2881 accgagcgaa gctggtagta atcaaaggcg agctcggctt cgacgctcaa acgcacgttt
     2941 tgtaaatctg ccgcagtggc ctgtgcttcg gaagtgctcg cgaccacggt gttgcgaatg
     3001 cgtccccaga aatccggttc ccacgaggcg tcgaatggga gcgtgtagtt ggtgttcatg
     3061 cgcccggggg acacgttcga gtgcgtggct gcggctgatt gtcgcgacac gatgatcgat
     3121 gggctcgtcg tgacagttgg gaagtattca gaacgagatt ctttcacgag cgcgcgtgcg
     3181 gcgcggaagt ttgcgtcagc gagcgcgatg ttttgattgg ccgtattgag ctgctcttcg
     3241 agactattca attgcggatc gttgaacatc tcccaccact tgccgcggat cacggcatcg
     3301 ctcgggtgcg cgaatttcca gtcgtcggtt tccttgaaag cgtcgggcgc gatttctttg
     3361 taagccgctg gcgtagcgac cgccggcttc gcatacttcg gcgccttgtc acaacccttt
     3421 aacaggaaca tcgcgatcag cgtcacgccc agaattttaa aatgaataga cattgccatc
     3481 tcttttttag ccgcggatct acacggataa agactcggat ctgttctgat ccagtattgt
     3541 tatccgtgtc atccgtggct taatacgatc caaatacaga tagaccacgg gggtcgtgaa
     3601 taacgtcagc atctgactca caagcaatcc tccaatgatc gaaattccca acggtcgtct
     3661 gagctcggca cccgtgccag ttccgagcgc gagcggcaac gcgccgagca gcgccgccat
     3721 cgtcgtcatc atgatcggac gaaaccgcag catgcacgct tgaaagatcg cgtcgcgcga
     3781 gctcttccct tcaccgcgtt cagccgccag cgcaaaatcg atcatcatga tcgcgttctt
     3841 cttcacgatc ccgatcagca ggatgattcc gatcaacgca atcaggctga gatccatgtg
     3901 cgtcacgagc agcgcgagca ccgcgcctac ccctgccgac ggcaacgtcg agagaatcgt
     3961 gatcgggtga atgtaactct catacagaat gccgagcacg atgtagacgg ccagcaacgc
     4021 ggcgacgatc agaagcggct cactcgccag cgacgtctga aacgcctgcg ccgtacccgc
     4081 gaaacttcca tgaatcgtcg ccggcatacc gatgctctgt tccgtttgat tgatcgcctg
     4141 caccgcatcg ctcagcgcca cacccggcgc gagattgaaa gagaaagtaa cagccggaaa
     4201 ctggccctgg tgattaactg ccagcggcgc tgtcgcgggc gcgtaatgtg caaccgcact
     4261 caacggcacg actccaccag ccgcagcatg cagatagatc tctttcaacc cttccggact
     4321 ctgccaaaat cgcggctcga cttccatcac cacgtgatac tgatttagct gcctgaaaat
     4381 cgtcgatact tgccgttgtc caaacgcatc gtagagtgtc gagtcgatgt cttgcggcgt
     4441 gattccgagt ctttgggccg tcgcgcgatc gtaatcaagc gacgcttgca agccgctgtt
     4501 ctgctgatcg ctgttgacgt cagtcacgat tgagaccttg cgtaactcct gcatcagaat
     4561 cggcgcccac ttcacgagat catcaagatt ctcactctga atcgtgtact ggtattgcgc
     4621 gttaccacca cgaccgccga cgcgcaggtc ctgcgtcgct tgtaaaaaga ctgacgcgcc
     4681 ttcgaccgtt gcgagctgac gtcgcaagcg acggatgatc tcgtcagcac tgactttgcg
     4741 ctcgtccaac ggcttcaacg agatgaacat tcttcccgtg ttcgtcgtcg tgccaccgcc
     4801 gctccccgtg aacgcgacaa cattcgccac ggccggatcg ctcttaatga tgtcgacaaa
     4861 gcgcgccatc cgttgtctca tcgccgtgaa cgacgaatcc tgcgcgccct gaatgccacc
     4921 ggcgagacgg cctgtgtcct gttgcggaaa aaatcctttc ggcacgatcc agagcaggta
     4981 cgcgttcacg ccaatcgtga tcagcagcac taacaacatc ggcaagggat gtcgcagcac
     5041 ccacgtcaaa ctccgttcgt acaaactcag aatccactga aacacgcgct cgctcgcgcg
     5101 atatagtcgt ccatgttcct cattgtgttt caggaggcgc gaacacatca tcggcgtcgt
     5161 cgttaacgac accacgagcg aaacgagaat cgccaccgag agcgtcaccg cgaattcgcg
     5221 aaacagacgt ccgacgattc cgcccatcaa cagcaacggc gtgaacaccg caacgagcga
     5281 gatgctgatc gagagcaccg tgaacccgat ttcctttgca cccagcaggg ccgcttgcaa
     5341 cggccgcatt ccctgttcga gatggcgcgt cacgttttcg attacgacga tcgcgtcgtc
     5401 cacaacgaag ccggtggaga tcgtcagcgc catcagcgag agattgtcga gactgtagtg
     5461 acacaggtac atcacgccaa aagtcccgat gagcgacacc ggcaccacga cggccggaat
     5521 gatcgtcgcg cgtggatttc tcaggaagac aaacacgacc atgatcacga gaccgataga
     5581 gatgatcagc gttcgttcca cgtctctgac tgacgcgcgg atcgtggttg tctggtccat
     5641 cacgaccgtg aggtcaattg cggattgaat cgatgccttc aattgcggca gcgcggcgcg
     5701 aacgctgtcg actgtgtcga taatattcgc gccgggctga cgaaagatta tgacgagcgc
     5761 gcacggtttt ccattcgcga gtcccgcgga acgcaggttc tcgaccgaat cctgcacgtt
     5821 cgcgatgtca gacaatctga tcgcggcgcc gttgctgtaa ccgacgatca gcgggcggta
     5881 gccggcggct tgcaaaagtt gatcgttgac ggcgatgtcg gaagtggtct tgttgtttgc
     5941 gagctgaccc tttgcgaggt tcgcgttttg ctggctcagc atgttgcgaa cgtcttcgag
     6001 accg
//
LOCUS       DASSFQ010000303         4953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_289373, whole genome shotgun sequence.
ACCESSION   DASSFQ010000303 DASSFQ010000000
VERSION     DASSFQ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289373"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VFH96_10885"
     CDS             <1..916
                     /locus_tag="VFH96_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6974522.1"
                     /translation="SFNGTENPSWDVINGEALLSPGRPLPVVVNRNGQEVSLTITPTT
                     HMENGEAAGTLDFIPDYGGLPVVVSGVAANSPAEAAGLKDNDRFITIGGEAVRSSAQV
                     TEFVRNHPTERIPITVQRDGQTVQLTTDEQRIDGRLGFGVQERYPLHAANIGSATKYA
                     YDQNIQILRLTGKALGQVFTGQRSVRNTLSGPIGIYQATQKSIEKMGWDGVFGMLGFL
                     SLNLGIFNLLPIPVLDGGAIFLMLIEGLLALIGLSISSAVRDRIQQVGFVMVLLLMVF
                     VISNDLLKTFGGNRQEEKPPPAASAPAK"
     gene            920..1996
                     /gene="ispG"
                     /locus_tag="VFH96_10890"
                     /gene_synonym="gcpE"
     CDS             920..1996
                     /gene="ispG"
                     /locus_tag="VFH96_10890"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET6974523.1"
                     /translation="MRKTRPVQVKHIQIGGGAPVVIQSMTKTDTSDVGATVAQIEEMV
                     RAGCEIVRLAVPDNDAAVALKEIRKRVPDVPIVADIHFHYKLALMALEAGIDKLRLNP
                     GNIGAHERVREVVRAAQVQKVPIRIGVNGGSLEKDLLKKYGTATPEAMVESALRHIQI
                     LEDLDFTDIIISLKASDVHRTVAAYRLLAEKVDYPFHLGVTEAGTSFSGTVKSSIGLG
                     ILLHEGIGDTIRVSLAAEPQEEVRVAWEIVKSLGLRTRGVTVVACPTCGRLDVDNFVG
                     IVEEIERRLAHVEEPLHLSIMGCAVNGPGEAHDSQLGITFGRGVGMIFKDGVPMRKVA
                     GENIVEAFVMETEKLLSEIKTSHG"
     gene            complement(2006..3070)
                     /locus_tag="VFH96_10895"
     CDS             complement(2006..3070)
                     /locus_tag="VFH96_10895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET6974524.1"
                     /translation="MFDELTGNARVKGILKRMLVADRLPGAMLFTGEEGIGKKLFALE
                     IARALNCRTPKDHEACGVCSSCVRIRKLNYPTRDDADEWTQIIWTDYADVGLVVAPRR
                     VLRVEQMRQIEKEANFRPFEGKARVFLIDEADKLNDASANALLKVLEEPPKTSHLILI
                     TARPAMLLPTILSRCQMIRFSPLTPDEITSHLVKNDIDTKTARLRARAAGGSMGRALS
                     GDLVTFTSQRKAMLKVLNALVLSDDRAQLLRSAEQLNEAQYKDEFEERLDVLETLIRD
                     AWMLSLGVDTSRIVNEDLSAELKEIAQKLDPSRAGDWILQIEDLREQLIVNVNRKITT
                     DSLFLVMAGDVAAGKRPKVR"
     gene            complement(3063..3734)
                     /gene="tmk"
                     /locus_tag="VFH96_10900"
     CDS             complement(3063..3734)
                     /gene="tmk"
                     /locus_tag="VFH96_10900"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET6974525.1"
                     /translation="MPGTFITFEGIDGSGKSTQLRLLGNFLKANGCDALLTREPGGTQ
                     LGLRLRAALLDAAEEVDPLTELLVFAADRAQHVRRLVRPALDAGRLVISDRYADATVA
                     YQGAGRGFSPKLISQIVQLATEGLKPDLTLLFDLTIEESTNRTARRSTGKTATRITRD
                     RLDIEHADFHARVRDAYLQIALAEPERVKLIDTSGPVERTQERLREIIIPYLQRRGHF
                     AVNYV"
     gene            complement(3780..4079)
                     /gene="hfq"
                     /locus_tag="VFH96_10905"
     CDS             complement(3780..4079)
                     /gene="hfq"
                     /locus_tag="VFH96_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET6974526.1"
                     /translation="MSDGKQAPQNIQDAFLNTVRREKASVIIYLTNGAKLTGRIRSFD
                     KFSVLLESGSQEQLIFKHAISTISQTRRATGDLHSQANAETNPREARHDVNSPEA"
     gene            complement(4182..>4953)
                     /gene="miaA"
                     /locus_tag="VFH96_10910"
     CDS             complement(4182..>4953)
                     /gene="miaA"
                     /locus_tag="VFH96_10910"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET6974527.1"
                     /translation="GVPHHLIDFVSPNINYTAVEWAREATRTIEEIESRGRVPLLVGG
                     TGFYLRALRQPLFVSPPTDESLRRRLNQIRNRRGAEHLHKVLRRLDPIAASQLYPRDW
                     PRVQRAIEVYLQTGRSIVDQQPERPEPHESSRRLRILALNPPRDELYKRINERTEAHF
                     RAGLVDEVRQLLDRGFSPDSNALGAHGYRRVVEYLKGLRDLESALEQTKLDVRHYAKR
                     QLTWFRHEADVEWFDGFGEENCVLRSVLESIFAGARSK"
BASE COUNT         1035 a         1373 c         1458 g         1087 t
ORIGIN      
        1 ctcgttcaat ggcacagaga acccgagctg ggacgttatc aatggcgaag cactgctctc
       61 gccgggtcgt cctttgccgg tcgtggtgaa tcgaaacggt caggaagtct cgctgacaat
      121 cacgccgact acgcacatgg agaatggcga agcggccggg accctggatt tcattcctga
      181 ttacggtggc ctgccggtgg tcgtctcagg tgtggctgca aactcaccgg ccgaagcggc
      241 tggtctgaaa gacaacgatc gctttatcac gatcggcggt gaagcggtgc gaagctcggc
      301 gcaggtgacg gagttcgtcc gcaatcaccc caccgagcgg attccgatca cggttcaacg
      361 tgacggccag acagtacaac tgaccacgga tgagcagcgc attgatggga gactgggatt
      421 cggcgtccag gagcgatatc cactccacgc ggccaatatc ggctcggcga caaagtacgc
      481 ctacgatcaa aacattcaaa tcctgcgttt gacaggcaag gcactcggac aagtcttcac
      541 cggtcaacgc tcggtccgca acacgttgtc aggcccgatc ggaatctacc aggcgacgca
      601 aaaatcaatt gagaagatgg gctgggacgg cgttttcggc atgctcggtt ttctcagtct
      661 gaacctcggt atcttcaacc tgctgccgat tccggtgctc gacggcggcg caatcttcct
      721 gatgctgatc gaggggctgc tcgctctcat cggtttatcg atctcttccg cggtgcgcga
      781 tcgtattcag caagtaggat tcgtgatggt gctgctgcta atggttttcg tgatcagcaa
      841 cgacctgctg aagacgtttg gaggcaatcg gcaagaggag aagccgccgc cggctgcttc
      901 tgccccggct aaatagctca tgcgcaaaac gcgaccagtt caagtcaagc atattcaaat
      961 cggtggtggc gctcccgtag tgattcagtc gatgactaag actgacactt cggacgtggg
     1021 cgctaccgtc gcgcagattg aggagatggt ccgcgcgggt tgtgagatcg tcagactcgc
     1081 cgttccggat aacgacgctg ctgtggcgtt gaaagagatc cggaaacgag tgcccgacgt
     1141 tccgatcgtc gccgacattc acttccacta caaactcgcg ttgatggcgc tggaagccgg
     1201 catcgacaag ctgcgactca atcccggcaa catcggcgca cacgagcgtg ttcgtgaagt
     1261 ggtgcgcgct gcgcaggtgc agaaagtgcc tattcggatc ggtgttaacg gcggctcgct
     1321 ggaaaaagat ctgctgaaga agtacggcac ggccacaccg gaagctatgg tcgaatcagc
     1381 gctcagacac atccagatcc tcgaagatct cgacttcact gacatcatca tctcgctgaa
     1441 ggcttctgac gtgcatcgca cggttgcggc ttatcgacta ctggctgaga aggttgatta
     1501 tccgtttcat ctcggcgtca cggaggctgg gacttcgttc agcggcacgg tgaaatcgtc
     1561 gatcggactc ggaattctgt tgcacgaagg cattggcgac acgattcgcg tctcgctggc
     1621 tgctgagcca caagaagagg ttcgtgtagc gtgggagatc gtgaagtcgc ttggattacg
     1681 gacgcgtggc gtgacggtgg ttgcttgtcc cacgtgtggg cggctcgatg ttgataactt
     1741 cgtcgggatt gttgaggaga tcgagcgccg gctggctcac gttgaggagc cgctgcacct
     1801 ttcgatcatg ggttgtgcgg tgaatggacc gggtgaagcg cacgactcgc agctcggaat
     1861 cacgtttgga cgtggtgtcg ggatgatctt caaagacggc gtgccgatgc ggaaggtggc
     1921 tggggaaaat atcgttgaag cgtttgtgat ggagactgag aagttacttt ccgagataaa
     1981 aaccagccac ggataacacg gatttttaac ggaccttcgg ccttttaccg gccgcaacat
     2041 cgcctgccat taccaggaat aaagagtcgg tcgtgatttt gcgattgaca ttgacgatca
     2101 gttgctcgcg taaatcttca atctgcaaga tccaatcacc tgcacggctc ggatcgagct
     2161 tctgagctat ctctttcaac tcggctgaca aatcttcgtt cacgattcgc gacgtgtcga
     2221 cgccgagcga cagcatccat gcatcgcgaa tcaaagtctc gagcacgtcc agtcgctctt
     2281 caaattcgtc tttgtactgc gcttcgttga gttgttccgc cgatcgcagc agttgtgcac
     2341 gatcgtcgct cagtacgagc gcgttgagta ctttcagcat cgctttccgc tgcgacgtga
     2401 acgtgacgag atcgccggaa agcgcgcggc ccatgctgcc gcccgccgcc cgcgctcgca
     2461 aacgtgccgt cttcgtatcg atatcgtttt taacgagatg actcgtgatc tcgtccggcg
     2521 tcagcggcga gaagcgaatc atctggcaac gcgagagtat cgtcggcaga agcatcgccg
     2581 gacgcgcagt gatcaagatc aggtgcgacg tctttggtgg ctcttcgagc actttcaaaa
     2641 gcgcatttgc ggaagcatcg ttgagcttgt cagcctcgtc gatcaaaaac acgcgcgcct
     2701 tgccttcgaa cggtcggaag ttcgcttcct tctcaatctg gcgcatctgc tcgacacgca
     2761 gcacacgcct cggcgccacc acgagcccga catccgcata gtcagtccag atgatctgcg
     2821 tccactcgtc tgcatcatca cgcgtcgggt agttaagctt cctgatgcga acacacgacg
     2881 aacagacacc gcacgcttca tgatctttcg gtgtgcgaca gttcaaagca cgagcgattt
     2941 cgagcgcgaa gagtttcttg ccgatgcctt cttcgcccgt gaacagcatc gcgccgggaa
     3001 gccgatctgc gaccagcatc cgtttcaata ttcctttgac tctggcgttt cctgtcagct
     3061 cgtcaaacat agttcaccgc aaagtgaccc ctcctttgca gatagggaat gatgatctct
     3121 cgcaaacgtt cctgcgtgcg ctcgactggt ccactcgtat cgatcaactt cacacgctcc
     3181 ggttcggcga gcgcgatctg cagataggca tcacgcacgc gcgcatgaaa gtcggcgtgc
     3241 tcgatgtcta aacgatcgcg cgtgatccgc gtcgcggttt tcccggtcga acgccgcgcg
     3301 gtgcgattcg tcgattcttc gatcgtcaga tcgaaaagca gagtcaggtc gggtttcaga
     3361 ccttctgtcg cgagctgaac gatttgcgag atcagcttag gcgaaaatcc tcgtcccgcg
     3421 ccctgatacg cgactgtcgc atcggcgtaa cgatccgaga tcacgagccg gccggcgtcg
     3481 agcgccggac gcacgaggcg ccgcacgtgc tgagcgcgat ccgccgcaaa caccagcagc
     3541 tcggtcaatg gatcgacttc ctccgccgcg tccagcagtg cagcccgcaa gcgcagtccg
     3601 agctgagtgc cgccgggctc gcgcgtcagc agcgcatcac aaccgttggc cttcaggaaa
     3661 ttgcccagta aacgcaactg agtcgacttc ccactgccgt cgataccctc aaaagtgatg
     3721 aaagttccag gcacacgtcc cctctaccag tgaaatgctc gcgagagttt aatcgaatgt
     3781 cacgcctcag gcgagttgac atcatgacgg gcttcgcgcg ggtttgtttc cgcattcgct
     3841 tgtgaatgca ggtcgcccgt cgcgcgtcgc gtctgtgaga tcgtcgagat ggcgtgtttg
     3901 aaaatcagtt gttcctgtga ccctgattcg agtaggaccg aaaacttgtc gaaactgcga
     3961 atacggcccg tcaacttggc gccgtttgtc aggtaaatga tcacagaagc tttttcacgg
     4021 cggaccgtgt tgagaaaagc gtcctgaata ttttgtggtg cctgtttacc gtccgacatg
     4081 ctgaaacctg ggggtggaat tgtagaccgc gcggttgcaa tgagattaca acacggatct
     4141 ttacctgaca agagatagct ggagatgtga gagaaagatg attatttgga ccgtgcgccg
     4201 gcgaaaatag attccaatac cgaacgcaaa acgcagtttt cctcaccaaa accgtcaaac
     4261 cactcgacgt cagcttcatg gcgaaaccat gtcaattggc gtttggcgta gtggcgcacg
     4321 tcgagtttgg tctgttccaa cgcgctttcg aggtctcgca agcccttcag gtactccacc
     4381 acgcgccggt agccgtgtgc gccgagggcg tttgaatcgg gcgagaaacc acggtccagc
     4441 aactggcgca cttcgtcgac cagaccagcg cgaaagtgtg cttcggtgcg ctcgttgatg
     4501 cgtttgtaga gctcgtcgcg cggcggatta agcgccaaga tacgcagacg ccgcgagctt
     4561 tcgtgtggct ccgggcgctc gggctgctgg tccacaatcg agcggcccgt ttgcaggtag
     4621 acctcgattg cgcgttgcac gcgcggccag tcgcgcgggt aaagctggct cgcggcaatt
     4681 ggatcgagac ggcggagcac tttgtgcagg tgttccgccc cgcgtctgtt acggatctgg
     4741 ttgagacggc ggcgcagaga ttcgtcagtt ggtggactga cgaaaagtgg ttgtcggagt
     4801 gcgcggaggt aaaagcccgt gccgccgacg aggagtggga cgcgtccgcg gctttcgatt
     4861 tcctcgatcg tcctggtggc ctcgcgggcc cattcgacgg cggtgtagtt gatgtttggt
     4921 gagacgaaat cgatcaggtg atgtgggacg cct
//
LOCUS       DASSFQ010000304        10914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_289542, whole genome shotgun sequence.
ACCESSION   DASSFQ010000304 DASSFQ010000000
VERSION     DASSFQ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289542"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFH96_10915"
     CDS             <1..606
                     /locus_tag="VFH96_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974528.1"
                     /translation="SIDSYMSLVLFLAMNDRFADASQVFAAYDQHKDTDVVLIAEVIR
                     QLYYDSEGARAVKLADTEPARVKGSAEANLILGKIHLEDGRYAVALNHLNLSAQIDKT
                     LSSPYVAMATIHRKQSRWGAALKAADQAIKLDEEDCEAYYERACALARLGRLRDAMAA
                     LEKSVDLDPDHLEWIPDEVDLKPLSSLPAFKKLLAPSPEKP"
     gene            723..1208
                     /locus_tag="VFH96_10920"
     CDS             723..1208
                     /locus_tag="VFH96_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974529.1"
                     /translation="MKHKTLPLLLVLVLLSSHAPLAFSQQTTSLSDWATVKQIKTNER
                     LLVRQKNGKEIKGEMIEASDTTLTIDRDGKPFSIARNDVRQVYVVSGTAKKGKWALIG
                     AGIGAGAGAGIGATKYSNSVDDSQIYIPIGLIIGAGSGALGGLLFGQSTRKRELVYAA
                     N"
     gene            complement(1564..1800)
                     /locus_tag="VFH96_10925"
     CDS             complement(1564..1800)
                     /locus_tag="VFH96_10925"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HET6974530.1"
                     /translation="MSSELTEEFLKEEYSDEDWWVAHGYLNPDDVPVPDWHLAILEER
                     MTRYEKEPVEWIPWEAIEKELMQEVEDEIKRRKK"
     gene            complement(1937..4912)
                     /locus_tag="VFH96_10930"
     CDS             complement(1937..4912)
                     /locus_tag="VFH96_10930"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6974531.1"
                     /translation="MNPRVGIYLLLTFIFAASLLLVSSSAISDEPASEGRSITPAGSL
                     VQDLTTRQAAVGALPVDFVRSPDKLGPGGEGRFLVSVNSGYGIQFNAAGNRGQQSLSV
                     IDLNAKPAAVVQNVYFPSPQSANVGVAFSPVPQDDGSYPLYVSGGFENKIWIFRFQAE
                     NQPPITPASPGPNTNVEAPFIDVSGFASAANSPRYNSDRAPVYPTGIAITPDGNTLFV
                     ANNLSDSLGVIEDVRGRRKLTRVDLRRDGDGEQFIYPYAVAVVPHQSSVKAYVSCWND
                     GTVAVVQAGKLVSRVAVGRHPTAMLLNPERTRLYVANSNDDSVSVIDTQTDKEIERIN
                     VRLSEGMPRGNSPEGLALRGDDLYVANAHSNSVAVVELSKKSRVKGFIPTGQYPSALA
                     LVGQTLFIGNGKGTGVQNSSLIVDNSGRVPNAPNERFPSGSGRAGGQGGQYSVALVVG
                     NISAVNTPDDPALTRYTQQVMRNNGLLGERQSRLFPGRSPIKHVIYVIKENRTYDQIF
                     GDLKQSGDGTKADSDPSLAIFGAGDAAARPNGQRQDISPNHRALALRFGLFDRFFVNS
                     EASPDGHNWSTAAFSTDYVDKAFRWNYSRRGRTYDFEGFNRLPNYEPLRGAPSLFGPG
                     ATTDDVANFMRRFIPYLQGSRDVAEPESLYLWDAAARAGLTYRNYGEFVATLSEADVK
                     AIRENRAKSYPDLSPTVSAFPTKKSLEGRVSTEYRNFDMDTPDAMTVDSYRAARESNG
                     SPLISNARGNSRLGVWLEEFRTGQLPNLSVIRLSNDHTDGISVRKPTPQFYVADNDYA
                     LGLMVEAVSNSPYWKDTAIVVVEDDAQDGPDHVDAHRSVALVISAYNKPGTLVHEFHN
                     TVSLIRTIELLLGIEPMNQLDATATPINIFRTEPDLRPYKVLLPNVALDNLVTLPSRD
                     VATAYWMQLTEEQDLERADMADPETLNRIIWFSVRGNMPMPSIARLPAFDAMRLGLQE
                     EREEIAEKVATKKHKDDVR"
     gene            5084..7384
                     /locus_tag="VFH96_10935"
     CDS             5084..7384
                     /locus_tag="VFH96_10935"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET6974532.1"
                     /translation="MSQLVRFLFGHEQAVFTNGRFGFDVRPSAIILALILLLAGVFIY
                     FIYLRPRVRLSKGTTVTLIALRATLLLLMVTLLLRPVVVVSSVIPRSSYVAMVIDDSV
                     SMKLHDVPGGATRLESVKQALLNTTSGQDSFLNRLEKKFKTNLYGFSGGLARLKDGND
                     LYGEGRTSDLAGALDETIKRSSGMPLSAVVLATDGASNVPRDLAAVLRELRARDIPVF
                     TVGVGNTSRPMDAELTRVNMPRRVLVGSRLNIETIVGLSGYSATKVLLNVKEDGRTVK
                     TEEFNLRGNDTQAVNLEITPTTPGIHRYTVEVTPLDGELTVDNNKQDSLVEVITGPLR
                     ILHVEGEPRWELGKIRESISPNEKNVTLVSLQRTGENKFYRQGVGNQTELATGFPKSE
                     EELFSYDGLIIGSVEAGFFTADELRNIEAFVARRGGGLLALGGRLAFDGGKFKGTTIE
                     ELLPVALTGGPVDDASSFTPVYKPVLTGAGQEHPITRLNDDRGANQKIWNELPPISVS
                     EVLSNVKPGASVLLEARRTEGSAQVAPLLVQQRYGRGQTLALTAADTWRWKMRMDSKN
                     NAHETFWRQMLRYLVSSTPQQVEIGTEKEVYALDDNVNIVADIRDKHFNPVADAKATA
                     RVTKPSGAVVEVPLKFTTLNSVNTYAGEFKADELGQHRIELVGTSQSLGTLSVTSNML
                     VSDLNREYYSAAQNSDLLKRISAETGGKYYTPAQAQSLLDDLIYRQTPYSERVTKDLW
                     DMPVNFLLIIGLLSAEWFLRKREGLA"
     gene            7463..8413
                     /locus_tag="VFH96_10940"
     CDS             7463..8413
                     /locus_tag="VFH96_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974533.1"
                     /translation="MKHAITLMLILSFTGIVCAQKSSDPNKFAIIINGAGGEAVYAKQ
                     FEEWTGQLSSVLSTKFGFDTKQLTVLKGATAEDVKRTFTTLKTQLDGNNVLFVFLIGH
                     GSFDGKESKFNLVGPDLAAADYNAMLSSLPTHRVVVFNMSSASGEFIKSLSAKGRIVI
                     TATKSGQETNATRFTGFLISALNATDADTDQDGHTSVLEAFVYANRLTADFYKQAGRL
                     ATEHAMFDDNGDGVGREKLEAGEGLLARATYLDSLSTGEAAASAAAGRLLKERTRLEG
                     EIEQLIARKGSMAETEYEATLERLFIELAKVNRSIKQAGS"
     gene            8506..>10914
                     /locus_tag="VFH96_10945"
     CDS             8506..>10914
                     /locus_tag="VFH96_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6974534.1"
                     /translation="MLFAISVCALAQTAPDTRAQLREYIETGHYTEAEAAAKKLLLKT
                     PDNGAVRHELAETLALTGRYTESITEFERAAADSAKTDNTADKLESDLRRAEVLTLIG
                     QEDRAKPLYESFVTYYTDRDPKSARELTLIARALVHLERYQDANDMYRSAIEADSNYL
                     EAQLGAAELFTQKYAYGDAALFLDDAFKINPNSARVFLAVARNKRLDGDAETSAALTK
                     ALTINPNLLEAIALKAGMALEASRIDEASAEIDKVLKINPRSLEAHSLRAAILYLQDK
                     DFEPEVAATLAIGPKYGEVYNTLSHYATITRRTEQAAQFARRATEISPRLWDAHLNLG
                     MALLRLGQMDTGREAVEKAFKGDPFNVWAKNSLDLLDSMRDFKETKRGPFIIKAAAQE
                     SDVLSPYAANLLEEAAAKLTAKYRFTPKGPIIIEIFNNHEDFAVRTLGIPGLGALGVC
                     FGFVIAQDSPSAREAGEFNWGSTLWHEYTHVITLQMTDYRIPRWFSEGLSVYEERRAR
                     PGWGDDWNPLFVRAFMERRWFKMADLDAGFQRPKSPQDVPIAYFQASQVCEFIAERYG
                     FDAILRMLAMYRDKAKTPDVLQQVLKLSESDFDREFAAYIEAKVKPLQQALLTPTNNA
                     ASLSKAEVLKALETQDTFALRIRAAELLAADGDTQAAVVHYIRALELFPFVSGKGNPY
                     ESLAKLLEAKGDREQAAKTLDSLVKTDENNLEALKTIARLRLALGERQQALDALQASF
                     FISPFDYKLHTQAGELSLEMKDYAKALTEFQVALALAPPNVAEANYNVAAAFHALGRQ
                     PEAKR"
BASE COUNT         2513 a         3013 c         3037 g         2351 t
ORIGIN      
        1 agtatcgata gctacatgtc gttagttctt ttccttgcca tgaatgatcg gttcgccgac
       61 gccagccagg tgtttgcagc ttacgatcag cataaggaca ctgatgtagt tctcatagcg
      121 gaggtaattc gtcagctcta ttacgattca gagggcgcgc gcgcagtaaa gcttgcagac
      181 actgagccgg caagggtgaa aggtagtgcg gaagccaacc tcatactcgg aaaaatccac
      241 ctggaggacg ggcgttatgc tgtcgcgctt aaccatctca atctgtcggc ccagattgat
      301 aagacactgt catcgccata cgtcgcgatg gcaacaattc atcgaaaaca gtcgcgctgg
      361 ggtgcggcgt tgaaagccgc cgatcaggcg atcaagcttg atgaagaaga ttgtgaagct
      421 tactacgagc gagcttgcgc tttggcgcgg cttggacgac tcagagacgc aatggcggct
      481 cttgaaaaat ccgtcgatct cgatcccgac caccttgagt ggatccctga tgaagttgat
      541 ctgaaaccgc tatcgtcgct gccggcattt aagaaacttc tcgcgccgtc acccgagaaa
      601 ccttagaagt ttgcgacagg tgtcggaaat ttctgcggcg tcgcaaaagg atctggcgca
      661 aactttcaac aagagtgccg gaacagtgct tgcacatgtt cacctttgaa aaggagaaca
      721 tgatgaagca taaaactctg cccctgttac ttgtcttagt actgctctct agccacgcgc
      781 cgctcgcttt ttcgcaacag accacatcac tgagcgattg ggccaccgtc aaacagatca
      841 aaaccaacga acgacttctc gttcggcaga agaatggaaa agagatcaaa ggtgagatga
      901 tcgaagcgtc agacacgacg ttaacgatcg atcgcgacgg caaaccgttt agcatagcgc
      961 gcaatgatgt gcggcaggtg tatgttgtct ccggcacggc gaagaaaggc aaatgggcct
     1021 tgatcggcgc aggcatcgga gccggtgctg gcgcaggtat cggcgccacg aagtattcaa
     1081 acagcgtcga cgacagccag atctacatcc cgatcggcct aatcatcggc gccggctcgg
     1141 gcgccctcgg cggcctgctc ttcggccaaa gcacgcgcaa acgtgagctg gtctacgcgg
     1201 cgaattagta gaacggcata agcctgttaa gtatgtccgt ttccttgcgt tatccaagga
     1261 gggtcggacc ggcgttgatg gacgatggct tcaacaccga tggcaccaac tcctactaag
     1321 tagaagatcg aataaggaaa ccgtttcagt cctgctcgcc gaaacccatt tctgcgaatt
     1381 ggaaatgcat acggcatgcg ggtgattcgc tccagagtag tgatgacttc cctcagaaag
     1441 cgagacgcca aattcggatc tatgtttagg taccagcgag ctgctgtgcg aatgtctgcc
     1501 atagcgcccg gtgagaggac gattctatat ttcatttgct ataagtgagg atctagccct
     1561 gtattacttc tttcgccttt ttatctcatc ttctacttcc tgcatcaatt ctttctcaat
     1621 cgcttcccac ggaatccatt ctacgggttc cttctcatat cgagtcatgc gctcttccag
     1681 aattgccagg tgccagtcag gaacgggaac atcgtccgga tttaggtaac cgtgagctac
     1741 ccaccagtct tcatctgagt actcttcttt taagaattct tcggttagtt ctgaggacat
     1801 tgttgcactc cttgtgggga gtgtatagca ccttaattct attctgcaag ccgattaatc
     1861 gtcttggtag tggttcactt tcgctgcaag tgtccgataa actttagttt gtcgcgacat
     1921 tccgacaagc taaagcttat cggacatcgt ctttgtgctt cttggtggca actttttcgg
     1981 cgatctcttc acgctcttcc tgtaatccga ggcgcatggc gtcgaaggcg ggaagacgcg
     2041 cgattgaggg catcggcatg ttgccgcgca ctgaaaacca gatgatgcgg ttcagggttt
     2101 cgggatcggc catgtcggcg cgctcgagat cctgttcttc ggtcaattgc atccagtaag
     2161 cagtcgcgac gtcacgcgac ggtagggtga cgaggttgtc gagcgcgacg ttcggaagca
     2221 gcactttgta aggtcgcagg tccggctccg tgcgaaagat gttgatgggc gtcgcggttg
     2281 cgtcgagctg attcatcggc tcgataccga gcaataattc gatcgtcctg atcaagctga
     2341 cggtgttgtg aaattcatgg accaacgtgc ccggtttgtt gtaggcgctg atcacgagtg
     2401 cgactgagcg gtgcgcgtcg acgtgatctg gtccatcctg cgcgtcgtct tcgacaacga
     2461 cgatcgccgt gtctttccag taaggactat tcgacaccgc ttccaccatc aacccgagcg
     2521 cgtagtcgtt atcggcaacg taaaactgcg gcgtcggctt gcgcacactg atcccatccg
     2581 tgtgatcgtt cgacaaccgt atgacggaaa gatttggcaa ctggcccgtg cgaaactcct
     2641 ccagccacac acccaatcgc gaattgccgc gagcattact gatcaatgga ctgccattac
     2701 tttcacgcgc ggcgcgatag gaatcgaccg tcatcgcatc gggcgtgtcc atgtcgaagt
     2761 tgcgatactc agtactaaca cgaccctcca gcgacttctt cgtcggaaac gccgaaacag
     2821 tcggcgaaag atccgggtac gacttcgcgc ggttctcgcg aatggctttg acatccgcct
     2881 ccgacaacgt cgcgacaaac tcaccgtagt ttcggtaagt cagaccagca cgcgctgccg
     2941 catcccacaa atacagcgac tccggctcgg cgacgtcgcg cgaaccctgc agataaggaa
     3001 taaaacggcg catgaagttg gccacatcgt ccgtggttgc tcctggtcca aacagcgaag
     3061 gcgcaccacg caatggctca taattcggca accgattgaa accttcaaaa tcgtacgtgc
     3121 ggccgcggcg actgtaattc cagcgaaacg ccttgtcgac gtaatcggtc gaaaacgcag
     3181 cggtggacca gttgtgcccg tcaggactcg cttccgagtt cacaaagaat cgatcgaaca
     3241 atccgaaacg aagcgcaagc gcgcgatgat ttggcgagat gtcctgacgc tgaccgttgg
     3301 ggcgcgcggc tgcgtcaccg gctccgaaga tcgcgagtga cggatcgctg tcagctttcg
     3361 tgccatcgcc cgactgtttc agatcgccga aaatttgatc gtacgtccgg ttttctttga
     3421 tcacgtagat gacgtgcttg atcggcgaac gtccgggaaa gagtctcgac tgtcgctcgc
     3481 caagcagacc gttgttacgc attacttgtt gtgtgtagcg tgtgagcgcc ggatcgtcag
     3541 gagtgttcac tgcgctgatg ttgccgacga cgagcgccac gctgtattgc ccgccctgtc
     3601 caccggcgcg accgcttccg gatggaaagc gttcgttcgg tgcgttgggc acgcggccgg
     3661 agttgtcgac gatcaatgaa gaattctgaa cgcctgtgcc tttgccgttg ccgatgaaga
     3721 gcgtttggcc caccagcgcg agggctgatg ggtattggcc ggtcgggata aaacctttga
     3781 ctctactttt cttgctgagc tcgacgactg cgactgagtt gctgtgcgcg ttcgcgacat
     3841 aaagatcgtc accgcgcagc gcgagtcctt cggggctgtt gccgcgtggc atgccttctg
     3901 acaaacgcac gttgatgcgc tcgatctctt tgtcagtctg cgtgtcgatc actgaaacac
     3961 tgtcgtcatt cgaattcgcg acgtaaagac gtgtgcgctc gggattcagc aacatcgccg
     4021 tgggatgccg cccaaccgcg acacgcgaaa ccaatttccc cgcctgaact accgcgacag
     4081 tgccgtcgtt ccaacacgac acgtaagctt taacagaact ctgatgaggc acgaccgcca
     4141 ccgcatacgg ataaataaac tgctcaccat caccatcgcg ccgcagatcg acgcgcgtga
     4201 gtttgcgtcg accgcgcaca tcttcaatca caccgagact gtcgctgaga ttgttcgcga
     4261 cgaagagcgt gttgccatcg ggcgtgatcg caattccggt gggataaacc ggcgcgcgat
     4321 cactgttgta gcgcggtgag tttgcggcag acgcgaatcc gcttacgtcg ataaaaggcg
     4381 cctcgacatt tgtgtttgga ccaggcgatg cgggcgtgat cggcggctga ttctcagctt
     4441 gaaagcgaaa gatccaaatc ttgttttcga atcctcccga gacgtaaagc gggtaagagc
     4501 cgtcgtcttg cggcaccggt gagaacgcaa cgccaacatt cgcactctgc ggcgatggaa
     4561 agtaaacatt ctgcaccacg gccgcaggct tcgcgtttag atcgatcacc gaaagcgact
     4621 gctgaccgcg attccccgcc gcattgaact gaatcccata accactgttt actgaaacca
     4681 ggaaccgacc ttcaccgccg ggtcccagct tgtcgggact gcgaacgaaa tcgaccggca
     4741 gcgcaccgac cgcagcctga cgcgtcgtca gatcctgaac cagcgaacct gcgggcgtga
     4801 tcgatcgtcc ctcgctggct ggttcgtcgg aaatggcgga tgacgaaacc aacaagaggc
     4861 tggccgcaaa aatgaatgta agaagcaggt aaataccaac tctggggttc attggctttc
     4921 gcagtttagc accgttgtat ccacagatta cacagatatc tgcgtaatct gtggatgctg
     4981 atacgaaaaa ataaattgcg tgacgaacgg atcgatgaaa acaaccgcaa actaccgtgc
     5041 ggctactaag cacaaacgcc gtgtataatg gcgttcacca aaaatgagcc aactcgtacg
     5101 gtttctgttc ggacatgagc aggccgtttt tactaacggc cgcttcggtt tcgacgtgcg
     5161 tccgagcgcc ataattctcg cgctcatact tctcctcgcc ggtgtcttta tttatttcat
     5221 ttacctgcgc ccgcgcgttc gtctctcaaa gggaacgacc gtcacgctca tcgcgctgcg
     5281 tgccacactt ttactgctga tggttacatt gctgttacgg ccagtcgtcg tcgtgtcgtc
     5341 agtgatcccg cgcagcagtt acgtcgcgat ggtgatcgac gactcggtca gtatgaaact
     5401 gcatgacgtt cccggcggcg ctactcgtct cgaaagcgtc aaacaggcac tgctcaacac
     5461 aacttccgga caagactcgt ttcttaatcg actcgaaaag aagttcaaaa caaatctcta
     5521 cggcttttcc ggcggcctcg cgcgactcaa agacggcaac gatctttacg gcgaaggacg
     5581 cacgagcgat ctcgcgggcg cattagacga gacgatcaaa cgttcttcgg gtatgccgct
     5641 ttccgctgtc gtccttgcaa ctgacggcgc ttcgaacgtg ccacgcgatc tcgccgcggt
     5701 tttaagagag ctgcgcgcgc gcgatattcc tgtgttcaca gtcggcgtcg gcaacacgtc
     5761 gcgcccgatg gatgccgagt taacacgcgt gaacatgccg cgacgcgtgc tagtcggctc
     5821 gagattgaac atcgagacga ttgtcggttt gagtggttac agtgcgacga aagtgctgct
     5881 gaacgtgaag gaagatggac ggacggtgaa aacagaagag ttcaacttgc gcggcaacga
     5941 cacgcaggca gtgaatctcg aaatcacgcc cacgacgccc ggcattcatc gttacacggt
     6001 tgaagtcaca ccgctcgacg gcgagctgac tgtcgacaac aacaaacagg attcgctcgt
     6061 agaagtaatc acaggaccac tgcgcatcct gcacgtcgaa ggcgagccgc gttgggagct
     6121 tggcaaaatt cgcgaatcga tctcgccgaa cgaaaagaac gtcacgctcg tttcgcttca
     6181 gcgcacgggt gagaacaagt tttaccggca gggtgtcggc aatcaaacag agctcgcgac
     6241 cggttttccg aagtcggaag aagaactgtt ctcgtatgac ggtctgatca tcggcagcgt
     6301 cgaggcggga ttctttacag cggatgagct gcgaaacatc gaggcgttcg tggcgcgacg
     6361 cggcggcggg ttgctggcgc tcggcggacg tctcgcgttc gacggcggca agtttaaggg
     6421 cacgacgata gaggagctgt tgccggtggc gctcacgggt ggtccagttg acgatgcgag
     6481 ctcttttacg ccggtttaca aaccggtgct gactggtgcg ggccaggagc acccaattac
     6541 acgtctgaac gacgatcgcg gtgcgaatca aaaaatttgg aacgaactgc cgccgatctc
     6601 cgtctccgaa gtgttgtcga acgtaaagcc gggcgcttcg gtgttgctcg aagcgcggcg
     6661 caccgagggt tcggcccagg tcgctccttt attagtgcag cagcggtacg gtcgcggaca
     6721 gacgctcgcg ctcacggcgg cggatacgtg gcgttggaaa atgagaatgg attcaaagaa
     6781 caatgcccac gaaacattct ggcgacagat gctgcgctat cttgtgagta gcacgcctca
     6841 gcaggttgag atcggtaccg agaaggaagt ttatgcactc gacgataacg tcaatatcgt
     6901 tgccgacatt cgtgacaagc atttcaatcc tgtcgctgat gcgaaggcga cggcgcgtgt
     6961 gacgaagcca tcgggtgccg tggtggaggt gccgctgaag tttacgacgc tcaactcggt
     7021 gaatacttac gcgggtgagt tcaaggcgga cgagttgggc caacaccgga tcgagctggt
     7081 tggtacgagt cagagtctcg ggacgttgag tgtgacgtca aacatgctcg tgagcgatct
     7141 gaatcgtgag tattacagtg cggcgcagaa tagtgatttg ttgaagcgca tctcggctga
     7201 gacgggtggg aagtactaca cgcccgccca agcgcagtcg ctgttggacg atctgattta
     7261 tcgtcaaacg ccttattcag agcgtgtaac gaaggatctc tgggacatgc cggtgaattt
     7321 tttgttgatt atcggattat taagtgctga atggttcctg agaaagcgtg aagggttggc
     7381 gtagttgttg acaggattta caagattttc aggatttaca tgttcatcct gaaatcttgt
     7441 aaatcctgtc taaaacttac ttatgaaaca cgctatcaca ttgatgttga ttctttcgtt
     7501 cacaggcata gtgtgcgcac agaaatcatc cgatccaaac aagttcgcaa tcatcattaa
     7561 tggtgctggc ggcgaggctg tttacgcgaa gcagtttgaa gaatggaccg ggcagttgag
     7621 ctccgtgctg tctacaaagt tcgggtttga tacgaagcag ctcacggtgt tgaagggcgc
     7681 gacggccgag gatgtgaagc gcacgttcac gacactcaaa acgcagctcg atgggaacaa
     7741 cgtgctgttc gtgtttctga tcggccacgg atcgtttgac ggcaaagaat caaagttcaa
     7801 tttggtgggg ccggatctcg cggcggctga ttacaacgcg atgctctcgt cgctgccgac
     7861 gcatcgagta gttgtcttca acatgtcgag cgcgagtggt gagttcatca agagtctttc
     7921 ggccaaaggc cggatcgtaa tcacagctac gaagagcggg caggagacga acgcaacacg
     7981 cttcacgggt ttcctgatct cggcgttgaa tgcgactgac gctgacaccg atcaggacgg
     8041 gcacacttcg gtgctcgaag cgtttgtcta cgcgaaccgt ctgactgcgg atttttataa
     8101 acaagctggc aggctcgcca ccgagcacgc catgtttgac gacaacggcg acggcgtcgg
     8161 acgcgaaaag ctggaagccg gtgaaggact gctcgcacgc gcaacatatc tcgattcgtt
     8221 gagtacgggc gaagcggcgg cgagtgcggc ggctggaaga ttactgaaag agcgaacacg
     8281 gttagaaggc gagatcgaac aactgatcgc gcgcaaagga agcatggcgg aaacggaata
     8341 cgaagcgacg ctcgaacgcc tatttattga gctggcgaaa gtaaatcgtt ccattaagca
     8401 agcaggatca tgataagcaa cagaaccgag cgcggtagcg ccgggtcaat ctccatccac
     8461 aggcgcttta ccagaattca cccggcgcta ccgcgctcgg ttctgttgct attcgcaatt
     8521 tctgtatgcg cccttgccca aaccgcgcca gatacgcgag cgcagctccg cgagtacatc
     8581 gaaaccggtc actacaccga ggccgaagcg gctgcgaaaa agctcctgct aaaaacaccc
     8641 gacaacggcg cagtccgtca cgagctcgcc gaaacacttg ccctcaccgg tcgctacaca
     8701 gaatccatca cggaatttga acgcgctgcc gccgactccg ccaaaacaga caacaccgca
     8761 gacaaactcg agagcgactt acgccgcgcc gaagtcctca cactgatcgg ccaggaagat
     8821 cgcgccaaac cactctacga atcgttcgtc acttactaca ccgatcgcga tccgaaatcc
     8881 gctcgcgagc taaccttaat cgctcgcgcc ctcgtccatc tcgaacgcta ccaggacgcg
     8941 aacgacatgt atcgctcggc gatcgaggcc gactccaatt atctcgaagc acaactcggc
     9001 gccgctgagc tgttcacgca aaagtacgct tacggcgacg ccgccctctt tctcgacgac
     9061 gctttcaaga tcaatcccaa cagcgcgcgc gtcttccttg ctgtcgcacg caataaacga
     9121 ctcgacggcg acgccgagac cagcgccgcg ctcactaaag cactcacgat caatccgaac
     9181 cttctcgagg cgatcgcact taaagccggc atggcgctcg aagccagccg catagacgaa
     9241 gcctccgctg aaatcgacaa ggtgctcaag atcaacccgc gctcactcga agcacactcg
     9301 ctgcgcgccg cgatattgta tctgcaggac aaagacttcg aaccggaagt cgctgcgact
     9361 ctcgcgattg gaccaaagta cggcgaggtc tacaacacgc tttcgcacta cgccacgatc
     9421 acgcgccgca ccgagcaagc tgctcagttc gcgcgtcgcg ccacggagat ctcgccgcgc
     9481 ctttgggacg ctcatctcaa cctcggtatg gcgttgctgc gtctcggcca gatggacacc
     9541 ggtcgcgaag cagtcgagaa ggcgttcaaa ggcgatccat tcaacgtctg ggccaagaac
     9601 tcgctcgacc tgctcgactc gatgcgcgac ttcaaggaaa cgaagcgtgg tccattcatt
     9661 attaaagctg cagcgcagga aagcgacgtg ctctcgccct atgccgcaaa tcttctcgaa
     9721 gaagcggcgg caaagttgac ggcgaagtat cgcttcacac ccaaaggtcc gatcatcatc
     9781 gagattttca acaaccacga agacttcgcg gtgcgaacgc tcggcattcc cggtctcggc
     9841 gcgctcggcg tttgttttgg atttgtaatt gcgcaggatt ctccgtcggc gcgtgaagct
     9901 ggcgagttca actggggcag cacgctctgg cacgagtaca cgcacgtcat cacgttgcag
     9961 atgactgact atcgcatccc gcgctggttt tcagaaggac tttccgttta cgaagagcgc
    10021 cgcgcgcgac cgggctgggg cgacgattgg aatcccctct ttgtgcgtgc gttcatggag
    10081 cggcgctggt tcaagatggc ggatctcgac gccggttttc agcgtccgaa gagtccgcag
    10141 gatgtgccga tcgcttattt ccaggcatcg caggtttgcg agttcatcgc cgagcgctac
    10201 gggttcgatg cgattcttcg catgctcgcg atgtatcgcg ataaggcgaa aacgcccgac
    10261 gtgctacagc aagttttgaa gctgagtgag agtgatttcg atcgtgagtt tgccgcgtat
    10321 atcgaagcga aagtcaaacc gctgcagcag gctttgctga cgccgaccaa caatgctgct
    10381 tcgctttcga aagccgaagt gttgaaggcg ctcgagactc aggacacttt tgcgctccgt
    10441 attcgcgccg ctgagttgct cgccgccgat ggcgacacgc aagctgctgt cgttcattac
    10501 atccgcgcac tcgagctctt cccttttgtc agcggcaagg gcaatcctta cgagtcgctc
    10561 gccaagctgc tcgaagcaaa aggcgatcgc gaacaggcag caaagactct cgactcgtta
    10621 gtaaagacag acgagaacaa tctcgaagcg ttaaagacca tcgcgcgtct tcgtctcgca
    10681 ctcggcgagc gacaacaggc gctcgacgct ttacaggcga gcttctttat cagtccgttt
    10741 gattacaagc tgcacacgca ggctggcgag ctgagtctcg agatgaagga ctacgcaaag
    10801 gcgttgacgg aatttcaggt ggcgctcgca ctggcgcccc cgaacgtggc cgaagcaaac
    10861 tacaatgtag ccgcggcgtt tcacgcgctc ggccgccagc ccgaagccaa gcgc
//
LOCUS       DASSFQ010000305         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_2083583, whole genome shotgun sequence.
ACCESSION   DASSFQ010000305 DASSFQ010000000
VERSION     DASSFQ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083583"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1041)
                     /locus_tag="VFH96_10950"
     CDS             complement(22..1041)
                     /locus_tag="VFH96_10950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6974535.1"
                     /translation="MRILLSSDHEYPASNGHGAGLHPKAFPSGSGYTLQDLLAKGLAE
                     QGHEVLYLVRRLRENFSAQSIPDGIKPIFEPDFDADVLHTISGRDASLIADWEARRKP
                     WVATCHMDEQTRGRERGSSTRNWIFVSQTLAHLHGHDRYVWNGIDPGEYIYRKTKDDY
                     FLFMSTMDWGTKKGLDIVLSMAKQLGLKLVVAGTGESYERIAQVQEMCRKVGARYVGD
                     VRGKEKAELLAGAKGFLFPTKLDEAFGLGMVEALMSGTPVICSDRGACPEIITPEVGF
                     VCRTDDDYLTAVDRITDLDPRACREKAMRDYHYHAMARNYVREYEIENARATREQARP
                     ATQHS"
     gene            complement(1046..1999)
                     /locus_tag="VFH96_10955"
     CDS             complement(1046..1999)
                     /locus_tag="VFH96_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6974536.1"
                     /translation="MKLIVSHLEAVRTYVTRELYYIQHDLITNHGWRHIEANYLWQRA
                     GSLEYKLRAVLGELPETIIFWEAYEVVLKHARQMQRLKCRKFFFADDLHWWNRRMREI
                     RVVVFALSDAVLSPYVYRWTSLYPELASKRLVWVPHSASTDFTLPFNADAENAIFLSG
                     AINEAYPLRRQMLQLHREGSYAIKYHRHPGYYCGYDYQRDARIGRGYAEKIRECRVGF
                     ADSVSPYAYIVAKFFEIPATGALLLADDTGGQQLKRLAFEPGRHYVPVSQKNLKAQIE
                     YVLAERNHDELDQIRRNGQRLVHEQHKTSDRARFINAVCAA"
     gene            complement(2009..>2883)
                     /locus_tag="VFH96_10960"
     CDS             complement(2009..>2883)
                     /locus_tag="VFH96_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974537.1"
                     /translation="LVVMAIIGYGDVMLGFRTGLTTFVTTPGALFAAAVGFCYAALCV
                     CTSLIFLDRRENTFCMPMHCGSSMLAGFTASLILALVYNQSMPTAAQNVSSSFLIIAL
                     GFLSPLHHFDRVVAKVKRFFGFASPLPQPQRLFLFVCSGNTCRSPMAAAIANAEIAAR
                     LGIPFKSLETVNARALSAGVSARVGSPITPEAAEVLQSLSVPVQPHAARNLTPELAAE
                     AEMIFCMTSTVRKAVIEMLPAVANKTLCLSDEADVPDPIGKGMAAYVDCARQIQRLVQ
                     MRFDKLNPRLSVAK"
BASE COUNT          641 a          792 c          804 g          646 t
ORIGIN      
        1 agagtccctg gatgatctgg ttcatgaatg ttgtgttgcc gggcgggctt gttcgcgtgt
       61 ggctcttgcg ttctcaattt cgtactcccg cacgtagttc cgcgccatcg cgtgatagtg
      121 ataatcgcgc atggcctttt ctctacaggc gcgcggatcg agatcggtga tccgatcgac
      181 tgcagtgagg taatcgtcgt cggtgcgaca gacaaaaccg acttccggag tgatgatctc
      241 aggacaagcg ccgcggtcgc tgcaaatcac cggcgtgccc gacatcagtg cttcaaccat
      301 tcccagccca aacgcttcat cgagttttgt ggggaagaga aaaccttttg cgcctgccag
      361 tagttcggct ttctctttgc cgcgtacgtc gccgacgtaa cgcgcgccga ctttgcgaca
      421 catctcctgc acctgggcga ttcgttcgta actctcgccg gtgccggcca cgacaagttt
      481 tagaccaagt tgcttcgcca tcgagagcac gatgtcgaga cctttctttg tgccccagtc
      541 catcgtcgac atgaacagga agtaatcgtc ttttgttttc ctgtagatgt attcgcccgg
      601 atcgattcca ttccacacgt atcgatcgtg gccgtgtaag tgagcgagcg tttgtgagac
      661 aaagatccag tttcgggtgg aactgccacg ttcgcgtccg cgagtctgtt cgtccatgtg
      721 acaggttgcg acccagggct ttctacgcgc ttcccagtcg gcgatcagtg atgcatcgcg
      781 accggaaatc gtgtgcaaca catcggcgtc aaaatccggc tcgaagatcg gtttgattcc
      841 gtcgggaatt gattgtgcgg agaaattttc acggagacgc cgcacgaggt aaaggacttc
      901 gtggccctgc tcagcgagac cctttgccag cagatcctga agcgtgtatc cggagcctga
      961 cggaaacgcc tttggatgca ggccggcgcc gtgtccattg ctggccggat attcatgatc
     1021 tgaggaaagt agtattcgca tcgtgctaag cggcgcaaac tgcgtttatg aatcgcgccc
     1081 gatcgctggt cttatgttgc tcgtgaacga gtcgctggcc gtttcgtcga atctggtcca
     1141 gttcgtcgtg gttacgttcg gctagcacat actcgatttg cgccttcagg tttttttgtg
     1201 aaaccggcac gtaatgtcga ccgggttcaa aggcgagcct tttaagctgt tggcctcccg
     1261 tgtcgtcagc caacagcaac gcgccggtgg ccggaatctc gaagaacttc gccactatat
     1321 acgcgtacgg cgaaacactg tcggcaaaac caacgcgaca ttctcgaatc ttctcagcat
     1381 aaccacgacc aatgcgtgcg tcgcgctgat agtcgtaacc gcagtaatag ccggggtgac
     1441 gatgatactt gatcgcgtac gaaccttcac gatgcagttg caacatctga cggcgcaacg
     1501 gatacgcctc gttgatggcg ccgctaagaa aaattgcgtt ctctgcgtcc gcattgaacg
     1561 gcaaagtgaa atccgtcgaa gccgagtgtg gcacccaaac gagtcgcttg ctcgcaagct
     1621 cgggatacaa actagtccag cgatagacat acgggctcag aaccgcgtca gacagtgcga
     1681 aaacgacgac tctgatctca cgcatgcgcc ggttccacca atgcagatcg tcggcgaaga
     1741 aaaacttgcg gcacttcagg cgctgcatct ggcgcgcatg cttgagaaca acctcgtatg
     1801 cttcccaaaa gatgattgtc tctggtaact cgccgagtac agcacgcaac ttgtattcga
     1861 gcgaacccgc gcgttgccag agataattgg cttcgatgtg tcgccagcca tggtttgtaa
     1921 tcagatcgtg ctggatgtag tacaactcac gagtgacgta ggttcgcacc gcctccaggt
     1981 gagatacgat cagtttcatt tgccacggct atttagccac ggataaacgc ggattcaatt
     2041 tgtcgaaccg catctggact agacgctgga tctgacgcgc acagtcaacg tacgcagcca
     2101 ttcctttgcc gatcggatcg ggaacgtcgg cctcgtcgct cagacagaga gttttgtttg
     2161 cgactgccgg cagcatttcg atcacggctt tgcgaaccgt gcttgtcatg cagaagatca
     2221 tttctgcttc cgccgccaat tccggcgtca agtttcgcgc tgcatgcggt tggacaggca
     2281 cactcaacga ttgcagtacc tcggcggctt ccggcgtgat gggcgacccg acccttgcag
     2341 aaactccggc actaagcgcg cgcgcgttca cggtttcgag tgacttaaac ggaataccga
     2401 ggcgcgcggc gatttccgcg ttggcaatcg cggcggccat cggcgagcga cacgtgttgc
     2461 cgctacacac aaatagaaag aggcgttgtg gttgcggcag tggtgatgca aagccaaaga
     2521 aacgtttgac cttcgccacg actcgatcga agtgatgcag tggcgataga aatccgagcg
     2581 cgatgataag aaaactcgaa ctgacattct gcgccgccgt cggcatgctc tggttgtaaa
     2641 cgagcgcaag aatcaagctg gctgtaaatc cggcgagcat gctcgaaccg cagtgcatcg
     2701 gcatgcagaa agtgttttcg cgtcgatcga gaaaaatcag tgatgtgcag acgcagagcg
     2761 cggcgtaaca aaaaccgact gcggcggcaa acaaagcgcc gggagtcgta acgaacgtgg
     2821 tcagcccggt gcgaaatccc aacatgacgt cgccgtaccc gatgatggcc atcacaacaa
     2881 gcg
//
LOCUS       DASSFQ010000306        15590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1774901, whole genome shotgun sequence.
ACCESSION   DASSFQ010000306 DASSFQ010000000
VERSION     DASSFQ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15590
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774901"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VFH96_10965"
     CDS             <1..958
                     /locus_tag="VFH96_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974538.1"
                     /translation="ENLVSQYLLGELPEEQQVEIEDRAFSDKDYLATITTVENDLIDE
                     YVRGELSAANRQRFESRFLASAERRKRVEFAKALRTVVSTAQEKNVSQTAIAWSWRES
                     FYAFLSGLNPAARLAFVAAAIVVVAGAALLFVETLRLRRQVTQLQAEKQAGQDFQQAL
                     EAERKRNAELNARLDQEKQQREQTDESLRQLTETPEAKNPAPPPVIATLTLLPGLSRG
                     GGQKPTLDLSNDARLVRLQIGIDPEEQYQSFAVELRTLAGRQVWNRENLAARTRRGSR
                     AVGLTLPATVLKSGEYELRLRGLTGGGGSEDVGFYYFNVRKK"
     gene            955..4308
                     /locus_tag="VFH96_10970"
     CDS             955..4308
                     /locus_tag="VFH96_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET6974539.1"
                     /translation="MRRTNTSFLALLIVLTTSQAVYSQTKILEPEKVIEQTIAAGETH
                     SYSMTLAAGMYGAIELNQKGLNQTLTVFSADGQKLRTADLASVGFSEEISLVAQDTTT
                     YRIEVKAAAKPARTGSYTLKLLQIHPATAEDRARVQGQTLAEEGMQFLIKQTATSKSE
                     ALDKFQKSIPVWQAAKDPDHEAQAFYYVAYTRNALGQYADAAEAAKKGRPLAQSTGNR
                     TLEAYLLDELGNSFNYRGERKQALDIFLEALKLRSDADPVGRSMTLNNIGIAYWGMGD
                     RRKSLDYMEEFVSVLHETGEVLKESTTLGNMCVLHSDLGEYKQAMELCNKALEMKREI
                     QDFAGQATTLSNTGNIYGCLGDYQKALDLYGQALAIHNRMKEESGAAIVINNIGWAYG
                     QMGEYEKAIEYYTQTIEPFRKRDNISGQAKAFNNIGVNYAKLNDFSKALEFHLQALAL
                     RSVENDRDGRATSLNNIANCYARLGDKQKALEYYTQAVALQRSVQNDRQLATTLRNVG
                     VFHREEGRIPTALEYLNEARTLSVKVGDRDTEASVLSEIAKIDLARGDLMSARKLIEQ
                     AIAQSESVRINLKSQGFRASYLASVRKYYEFEIEVLMRLHQQRPNEGFDAAAIEVSEK
                     SRARSLLELLREARAQIQQGVDPSLIERESHLRRLIAEAAERQTRLLSGKYTEEQALA
                     ASKEIDALTTEYDQVQARIRESSPRYASLVEPAPLSVAEIQKRVLDQDTLLLEYALGE
                     QKSFVWVVAHDSVKSFELPGRAAIEQGAKRFYQLLTERGAAMPNETLAQRKQRLDHVD
                     TDYPQAAASLSRMLLAPVAAELGQKRLLIVAEGVLQYVPFAALPAGDDAARPLIVDHE
                     IVNLPSASVLAMLREELGNRKPASKSVAVIADPVFTANDPRLATAVKTATNESPFVDA
                     QRSAAESGVNDLSRLRFSRQEADEIARLAGETRNLKAVDFAANRSTATDPRLGDYQIV
                     HFATHGLINNQHPDLSGVVLSLVDEQGRPQNGFLRLYDIYNLKLNADLVVLSACQTAL
                     GKEIKGEGLVGLTRGFMYAGAPRVVATLWRIDDRATADMMRRFYEAMLKDGLRPAAAL
                     RAAQLSMLRDRRWHDAHFWAPFTIQGEWR"
     gene            complement(4337..5023)
                     /gene="mtnC"
                     /locus_tag="VFH96_10975"
     CDS             complement(4337..5023)
                     /gene="mtnC"
                     /locus_tag="VFH96_10975"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HET6974540.1"
                     /translation="MSLRAILLDIEGTTTPIAFVHDVLFSYARDHVREFLAANPAAED
                     IALLREEHANDVSDAPPLGNEMESIAAYVEWLIKLDRKSTALKSLQGKIWRQGYQEGS
                     LKSQVFADVAPAFKRWRDRGLKISIFSSGSVLAQQLLFAHTEVGDLTPFIDSYFDTKI
                     GKKGDAESYQKIAEGMGLPPQEILFVSDVVAELDAANEAGMKTLLSLRPGNELQEERY
                     PSIRSFDEIR"
     gene            complement(5020..5556)
                     /locus_tag="VFH96_10980"
     CDS             complement(5020..5556)
                     /locus_tag="VFH96_10980"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6974541.1"
                     /translation="MAVVTIVNEDKKLTDPEEITASLASHGIDYERWTPSHPISDDAS
                     PAAILDAYAREIEALKARGGYVTADVIDVSVTTPGLDAMLAKFNREHWHDEDEVRFII
                     AGHGLFHIHTTTGAVLAIEVEAGDLIRVPRGTLHWFNLCTDRRIRAIRLFQDTAGWTP
                     HYTESRVDENFLPLCFSA"
     gene            complement(5566..6186)
                     /gene="mtnB"
                     /locus_tag="VFH96_10985"
     CDS             complement(5566..6186)
                     /gene="mtnB"
                     /locus_tag="VFH96_10985"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:TIGR03328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HET6974542.1"
                     /translation="MSDGFNEIAVGLAAVARGFHARGWLLGTSGNLSAVVQREPLQIA
                     MSPSGVDKGELRAEELLSIDENARIVGQQGGKPSDESLLHIRIVKERGAGAVLHTHSI
                     WNTILSDLYAADGGIKIEGYEMLKGLQGVRTHEHAEWLPIVENSQDMPALAETIGATL
                     NEHKAAHGFLLRRHGLYSWGENLAQAKRHIEIIEFLLEAIGRTRKF"
     gene            6215..6523
                     /locus_tag="VFH96_10990"
     CDS             6215..6523
                     /locus_tag="VFH96_10990"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HET6974543.1"
                     /translation="MRKSSEQSVLFVDDQLGDPVYSEVVFVGRGMATQADKLVTEIRA
                     LPDEEKLRVLDAILTDLDKPDQEIDQVWAEEARKRWSSYKAGHLQTVSYEELMRKYNR
                     "
     gene            6523..6819
                     /locus_tag="VFH96_10995"
     CDS             6523..6819
                     /locus_tag="VFH96_10995"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET6974544.1"
                     /translation="MNIRFLTLAQQEVDEAVAWLEERTEGTGVDFLDELDRIVRIVKS
                     YPFASPQVDREIRRRLFARFPYSLIYGIDHNTIVVIAVAHSRRAPHYWAERLKF"
     gene            7190..8263
                     /locus_tag="VFH96_11000"
     CDS             7190..8263
                     /locus_tag="VFH96_11000"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET6974545.1"
                     /translation="MELPVNPPILPMLAKRVSELPKAGAWIFEPKWDGFRALIFREGK
                     EVFIQSRDEKPLNRYFPELIDPILKQLPTHCVLDGEIVIAQKGALDFDSLQLRIHPAA
                     SRVKLLSEQLPASIVFFDLLSEGKKDLRNQPFEERRERLQSMLEAAKPPLHLTPATTD
                     FDVASDWFRRFEGAGLDGVVAKPVAGTYESNKRVMLKIKHERDCDCVVAGFRWHKKGD
                     RDRIGSLLLGLFNDSGNLQHVGVCASFTDQKRIELVEFLKPYRKNALENHPWKEWAGA
                     DLAKRIPGAQSRWSAGKDLSWEPLRVELVVEVAYEHMQGDRFRHTAQFRRWRTDKKPS
                     DCTYEQLEVVPPHEIELIFKTKS"
     gene            complement(8315..9619)
                     /gene="ligD"
                     /locus_tag="VFH96_11005"
     CDS             complement(8315..9619)
                     /gene="ligD"
                     /locus_tag="VFH96_11005"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET6974546.1"
                     /translation="MAKSKEEILTIDGREVRVTNPDKPYFSLQTKLTKLELVQYYLSI
                     APGALAGIRDRPIVLKRFVDGAEGQVFYQKRAPDNHPEWLRTVTLSFPSGRTADEVVV
                     DDAAGLVWVVNLGCIELHPHPVRSKDLDHPDELRVDLDPGPGVEWESVRLVALEVKAL
                     LEEMGLRGWPKTSGSRGMHVNVRIEPRWTFSEVRRAAVALSREIERRVPRLASSKWWK
                     EERHGVFLDYNQNAKDRTTCSAYSVRPLPDARVSTPLEWNEVEGCEPADFTVLTVPKR
                     FADLGNPHAEMDAAAGSLEKLLELADRDEASGLGDAPWPPHFAKMEGEAPRVAPSRAK
                     KPAAKKRVRMPLIVVANSPDKKAALEGLERWNKKYSKVSKLLAVDDVLVDTMRGRSST
                     WTRIRVNLRNVPEKIRPAQETPDPDDDPTREWREMHRRRSQD"
     gene            complement(9623..10087)
                     /locus_tag="VFH96_11010"
     CDS             complement(9623..10087)
                     /locus_tag="VFH96_11010"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6974547.1"
                     /translation="MMIRIATTVDAPQITAVINAAFRIAEEFFIDGNRITQAEVEESL
                     AKGAFLLAEMNGKLNGCVYVELRGARSYLGLLSVDPTCQQGGLGSRLMLEAEKYCRER
                     GSRGMDILIVSLREDLPSFYQRRGYVADGTTPFPPDVPTKIPCHFINMSKSL"
     gene            10244..11734
                     /locus_tag="VFH96_11015"
     CDS             10244..11734
                     /locus_tag="VFH96_11015"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6974548.1"
                     /translation="MTTKTVTETPPEARSGLDPWRIDVLPQAPRPHGLGWFSAIGPGV
                     IALGVSIGSGEFLLGPAAFVKYGLSLLWVVGVAALLQTLLNVELMRYTMATGEPIFTG
                     FMRTRPHATFWAWFYAALYFLQMGWPGWAGAAAGAVFFLFAKRLPVASEAGMVYGIGV
                     ALFLLCALILTIGRRIERTLEYLNWIMVSVIIGGLGILAVLFVPLSTWLAAVAGFGGF
                     DVKTGSFQLVPAGADFFLLGAFAAYAGGGGVANLTLSNWARDKGYGMSSIVGFIPAAV
                     GGKKVALAHSGSTFKPDAESLQRWRGWWRIIRVDQWGVYFTGAILGMMLPGLLYVTFV
                     PAGSDIRGLGIAAALAQAVATQKGAIFGGVIAALGVWLLFKTQLDILEAMTRGVTDIL
                     WAGSARIRAWRGGDVRVVYYSVLAVITVWGILALRLTQPIVLLALSANMAGIVFIISS
                     LHLLYVNTRLLPVELRPPWWRRLALVALSLFYGMFLMLWLWSLIMK"
     gene            11784..13625
                     /locus_tag="VFH96_11020"
     CDS             11784..13625
                     /locus_tag="VFH96_11020"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HET6974549.1"
                     /translation="MHYKFLVLALLLVVSRVSAQEKPQTFTAQGISVEFTATHPVAGE
                     EIKVQFKITDTNGAALSSLRPVAWIDQRQPNQPLDARVCREKVQAFLQPSFSSRPTLD
                     LNAYFILALNNEPNISVIDPLSGFGGSKLYTLIPLKSPGEDWVLADDNKRLYVSMPQV
                     NQVAVIDIPTWKVIANVDAGAMPTRVALQRDERYLWVGNDSEAASGVTVIDTTTNKVA
                     AQIKTGLGHHEIAFTDDDRLAFVTNKQTGTLSLIDIRKLTILKDLKVGSQPAAIAFST
                     LSQTAYVANEGDGTIAAISAGKQDVVSRMPTAPGLTAISIPSNGHYGLALNTAKSTAY
                     AFDLSSNRVVQTVPVGPGSDQLSFTKQFAYVRASGSEFVTMINMANIGKEANVTRFPA
                     GQRAPGESLSRSHAAAIVPAPEEGAVLVANPADKMIYYYTEGMAAPMGSFQNYKRDPR
                     ALLVMDNSLRETARGIYSTTARLNNPGSYDVAFLLDSPRLVNCFEIQVAQNPNAPTKV
                     QSAYKIEPVVKEAVARPGERFTLHFKVVDYKTGAAKAGLNDLSVLVFLAPGIWQQRDL
                     AKSSTDGVYETSFVPPSEGVYYVYFQSPSLGMQYNQSMPLTIQAAKP"
     gene            13663..14289
                     /locus_tag="VFH96_11025"
     CDS             13663..14289
                     /locus_tag="VFH96_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET6974550.1"
                     /translation="MKSIQHSYTWAMLTLVLLIAATVHAQEPTAPPQTVVPNTQENNA
                     QRYFTDTVLINQNGERVRFYTDLMKDKTVIIDTFFATCQASCLPMNRNLAQIQEGLGD
                     RLGKDVNILSISVDPGVDTPTLLKAYAKKLNAKPGWYFLTGDKESIEFILKKLGQFVD
                     DKNDHLNIFLIGNNRTGLWKKAFGLAKSEELMKVVESVVNDPGTAAKP"
     gene            14373..15089
                     /locus_tag="VFH96_11030"
     CDS             14373..15089
                     /locus_tag="VFH96_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET6974551.1"
                     /translation="MLLKVIALALLVFIPIPGVSNAQSTRKPAAKRVVYACPMHPDVT
                     SSKPGRCPKCKMQLRPVKPEPSPRPSPTPNPTLSFSSAKIPNTRILDQNGKQLNFYND
                     LIKGKTVAINFIFTTCTTVCPPLTATFRKVQQTATERGLDVKLISVSVDPVIDTPERL
                     RAFAEKFNADQGWTFVTGEKGDIDSLLQSLGVAVTNKNDHTPMILIGNDSLDYWTRAY
                     GLSSPTKLVDLIAEAARSVR"
     gene            complement(15133..>15590)
                     /locus_tag="VFH96_11035"
     CDS             complement(15133..>15590)
                     /locus_tag="VFH96_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974552.1"
                     /translation="IWTDVDGVQTADPKLVPGTRTVPIISYDEAAQLAVLGARVMHPK
                     MIEPVVPQRIPVSIRNSRAPERDGTLICAVSEPSAGVVKAIAHRMNATHALVGCVGDG
                     LNNGSASAAAVRQVDPTLEWKSTAPSNLVATVERERVPEIVRQIHDRLF"
BASE COUNT         3539 a         4370 c         4305 g         3376 t
ORIGIN      
        1 cgaaaaccta gtttcccaat acctgctcgg cgagctgccg gaagaacagc aggtcgaaat
       61 cgaagaccgc gccttttccg acaaagacta tctcgcaacg atcaccaccg ttgaaaacga
      121 tctgatcgac gaatacgtcc gcggcgagct ctccgcagcc aatcgacaac gcttcgagag
      181 tcgcttcctc gcctcagccg aacgccgcaa gcgcgtcgag ttcgcgaaag ccctgcgcac
      241 cgtagtttca actgctcagg aaaaaaatgt gagccaaaca gcgatcgcgt ggtcctggcg
      301 cgaatcgttt tacgcgttcc ttagtgggtt gaatccagcg gcacgcctcg cttttgttgc
      361 cgctgcgatc gtggtagtcg ccggtgctgc attgctgttc gttgaaacgc tgcgattgcg
      421 ccgtcaggtc acgcagctac aagccgagaa gcaggcagga caagactttc aacaagcgct
      481 cgaagccgag cgcaaacgta acgccgagct gaacgcgcgg ctcgatcagg aaaagcaaca
      541 gcgagaacaa actgatgagt cgttgcgaca gttaaccgaa acaccagaag cgaagaatcc
      601 ggcgccaccg cccgtgatcg caacgttaac gcttctgcct ggtctttcac gcggcggtgg
      661 tcagaagccg accctcgatc tttcaaacga cgcacgtttg gtcaggcttc aaatcggtat
      721 cgatcccgaa gagcagtacc agagttttgc ggtcgagctg cgcaccttag ccgggcgaca
      781 ggtttggaac cgcgaaaacc tggccgcgcg aacgcgacgc ggatcacgcg ctgtcgggct
      841 cacgttaccg gcaacggtac ttaagtcagg cgaatacgaa ctcagattac gcggcctcac
      901 cggaggcggc ggctctgaag atgttggttt ctattacttc aatgtgagaa aaaaatgaga
      961 cgcacaaata catccttcct cgccctcctg atagtgctca ccacctccca agccgtctac
     1021 tcccaaacaa aaattctcga acctgaaaaa gtaatagaac aaacgatcgc tgcgggcgag
     1081 acgcattcct acagcatgac gctggctgcg ggaatgtacg gagccatcga gctgaatcag
     1141 aaaggactga atcaaacgct cacggtcttc tccgcagatg gtcaaaaact caggaccgca
     1201 gatctcgcct ccgttggttt ttcagaggaa atctctctcg tcgcacagga tactacgacg
     1261 tatcgcattg aagtgaaggc ggcagccaaa cccgctcgca ccggaagtta tacgcttaag
     1321 ctattgcaga tccatccagc cacagccgag gatcgagcgc gtgtgcaggg acaaacgctg
     1381 gcggaagaag gtatgcagtt tttgatcaag caaactgcca cttctaaaag tgaagctctc
     1441 gataaattcc aaaaatcaat tccggtctgg caggcggcaa aagatcctga tcacgaagca
     1501 caggcatttt attacgtggc ttatacccgg aacgcattgg gccaatatgc tgacgctgcc
     1561 gaggccgcga agaagggccg tcctctcgcc cagtcgaccg gcaaccgcac tctcgaagct
     1621 tatttgctcg acgagctcgg caactctttc aattatcgtg gtgaacgcaa acaggcgctc
     1681 gacatctttc tcgaggcgct caaactccgt tctgatgccg atccagtagg gcggtcgatg
     1741 acgttgaaca acatcggcat tgcctattgg gggatgggcg accgcagaaa atcgctcgac
     1801 tacatggaag agtttgtctc tgtgttgcac gaaaccggcg aagtgctgaa agagtcgacg
     1861 acgcttggga acatgtgcgt gctccacagc gatctcggtg agtacaaaca ggcgatggaa
     1921 ctgtgtaata aggcgctgga aatgaagcgc gagatccagg actttgccgg tcaggcgaca
     1981 acgttgagta acaccggaaa catttatggg tgtttaggtg attaccagaa agctttggac
     2041 ctttacgggc aggcgctcgc gattcacaac agaatgaaag aagagtcagg cgcggcgatt
     2101 gtcattaata acatcggttg ggcctacggg cagatgggtg agtatgaaaa agctattgag
     2161 tattacacgc agacaatcga gccttttcgc aagcgggata atatatccgg ccaagcgaaa
     2221 gcattcaaca acatcggtgt taactacgcc aagctgaatg acttttccaa agcgctcgag
     2281 tttcacttgc aagcgctcgc tcttcgctcg gtggagaacg accgtgacgg ccgcgcgacc
     2341 tcgctcaaca acatcgcgaa ttgctatgcc cgtctcggag acaagcagaa ggcgctcgaa
     2401 tattacacgc aggcagtcgc gctgcaacgt tcagtacaaa atgatcgcca gcttgcaacg
     2461 acgctcagga acgtcggcgt ctttcatcgc gaggaaggcc ggatacccac ggcgctcgag
     2521 tatctaaatg aggcgcgcac gctcagcgtg aaggttggtg atcgtgatac tgaggccagc
     2581 gttttgtcgg agattgctaa gatcgacctt gcgcgtggcg atctcatgtc tgcgcgcaag
     2641 ttgatcgaac aggcgattgc gcagagtgag tcggtgcgta tcaatctcaa gagccagggc
     2701 tttcgtgcgt catatctggc gtcagtccgg aagtattacg agtttgagat tgaggtgttg
     2761 atgcgtttgc accagcaacg accgaacgaa ggatttgatg cggctgcgat tgaggtaagt
     2821 gaaaagagcc gggcgcgatc gttgctggag ttgttgcgtg aggcgcgtgc tcagatccag
     2881 cagggcgtcg atccgtcgct aatcgagcgc gagagtcatt tgcgacgatt gattgccgaa
     2941 gcggctgaac gacagacgcg tctgctgagt gggaagtaca cggaagagca ggcactcgca
     3001 gcgtcgaaag agattgacgc gctgacgacg gaatacgatc aggtccaggc acgcatacgg
     3061 gagtcgagtc ctcgttacgc ttcactggtt gagccggcgc cgctgagtgt tgcagagatt
     3121 caaaagcgag tgttggacca ggatacgttg ttgctcgagt atgcgctggg cgagcagaag
     3181 agttttgtgt gggtagtcgc gcacgactca gtgaagtctt tcgagttacc ggggcgcgcg
     3241 gcgattgagc agggcgcgaa gcgtttctac cagttattga ctgagcgtgg cgcggctatg
     3301 ccgaacgaga cgctggcgca gcgcaagcaa cgactcgatc acgtcgatac ggattatccg
     3361 caggcggcgg cgagtttgag ccggatgttg ctcgcgccgg ttgcggcgga gcttgggcag
     3421 aagcgactgt taatcgtggc tgaaggtgtg ttgcagtatg tgccgtttgc ggcgctgcca
     3481 gcaggtgacg atgcggcgcg gcctttgatc gtggaccatg agatcgtgaa tttgccgtcg
     3541 gcgtcggtgc tggcgatgtt gcgggaggag ctcgggaatc ggaagccggc gagtaagtct
     3601 gtggcggtga tcgcagaccc tgtctttact gcgaacgacc cgcgccttgc gaccgcggtt
     3661 aagaccgcta ctaatgagtc tccgttcgtt gacgcgcaga gatctgcggc tgagtcaggc
     3721 gtcaatgatc tgtcgcgact gcgattcagt cgtcaggaag cagacgagat cgcgcgcctc
     3781 gctggtgaaa cgcgcaacct gaaggctgtt gatttcgcag ccaatcgatc gaccgcgacc
     3841 gatccgagat tgggtgacta ccagatcgtt cacttcgcca cgcacggatt aatcaacaac
     3901 cagcatccgg atctttccgg cgttgtactt tcactcgttg acgaacaggg caggccacag
     3961 aacggttttc tgcgcctcta tgacatctac aaccttaagc tcaacgctga tctcgtagta
     4021 ctgagtgcgt gtcaaacggc gctcggcaaa gagatcaaag gcgaaggcct agttggtcta
     4081 acgcgtggct tcatgtacgc gggcgccccg cgcgtggtcg caactctctg gcgcatcgac
     4141 gatcgcgcca ccgccgacat gatgcgacgc ttttacgaag cgatgctgaa agacggtctg
     4201 cgtcccgccg ccgccctgcg cgccgcccaa ctctcaatgc tccgcgaccg tcgctggcac
     4261 gacgcccatt tctgggcccc attcacaatt caaggagagt ggcgctaagt ccgtgtattt
     4321 atccgtctaa tccgtgttac ctgatttcgt caaaactacg gatcgacgga tatctctctt
     4381 cttgtagctc attccctggt cgtagcgaaa gcaacgtttt catccccgct tcattcgccg
     4441 catcaagttc ggcgacaaca tcagacacga agagtatttc ttgcggaggg agccccatcc
     4501 cctccgctat cttttgataa ctctccgcat cacctttctt cccaatcttc gtatcgaagt
     4561 aactatcgat aaacggcgtg agatccccga cctccgtgtg cgcgaagagg agctgctggg
     4621 ccaacacact acccgatgaa aaaatactga tctttaaccc tcgatcacgc caacgcttaa
     4681 acgccggcgc cacatccgca aacacctgcg acttcaacga tccctcctga taaccctgcc
     4741 gccagatctt tccttgcaac gacttcaatg ccgtcgattt gcgatccagc tttatgagcc
     4801 actccacata cgccgcaatc gactccatct cgtttcccag cggcggcgca tcacttacat
     4861 cattcgcatg ttcttcacgc aagagtgcaa tatcttcagc ggccgggttc gcagccagaa
     4921 actcccgcac atgatcgcgc gcatagctga acaacacgtc gtgcacaaaa gcgatcggtg
     4981 tagttgtccc ttcgatatcg agcagtatgg cgcgtaggct catgcgctga aacacaaggg
     5041 aagaaagttt tcgtcaacgc ggctttcagt gtaatgaggc gtccagcccg ctgtgtcctg
     5101 gaataaacgg atcgcgcgga tgcgacgatc agtgcagaga ttgaaccagt gtagtgtccc
     5161 tcgcggcaca cggatcaaat caccagcttc aacttcgatc gccagcacgg ccccggttgt
     5221 cgtgtgaatg tgaaacagcc catgtccggc aatgatgaat ctgacttcgt cctcgtcgtg
     5281 ccagtgttcg cgattgaact tcgccagcat tgcatcgagc cctggcgtcg taacgctgac
     5341 atcgatcaca tcggcagtca cgtagccgcc gcgcgctttg agcgcttcga tctcgcgcgc
     5401 gtatgcatca aggatcgcgg ctggcgacgc gtcgtcggag attggatgtg aaggcgtcca
     5461 acgctcgtaa tcgatgccgt gactggcgag gcttgcagtt atctcctcag gatctgtgag
     5521 cttcttgtct tcgttaacaa tggttactac agccatcttg ttctcctaaa actttcgcgt
     5581 gcgtccgatc gcttcgagca ggaactcgat aatctcgata tgccgctttg cctgcgcgag
     5641 gttttcgccc catgagtaga gcccgtgacg tctcagcaga aaaccgtgcg cggctttgtg
     5701 ttcgttcagc gtcgcaccaa tcgtttcagc aagcgccggc atgtcctgcg aattctcgac
     5761 gattggtaac cactcagcat gttcgtgtgt gcgcacgccc tgcaaaccct tcaacatctc
     5821 gtagccttcg atcttgatgc cgccgtccgc cgcgtataga tccgagagga tcgtattcca
     5881 gatcgaatgc gtgtgcagca ccgcgccggc gccacgttcc ttcacgatac gaatatgcag
     5941 cagcgactca tccgacggct ttccgccctg ctgtcccacg atacgagcat tctcatcgat
     6001 cgagagtaat tcctcggcgc gcagctcacc cttatcgacc ccactcggac tcatcgcaat
     6061 ctgaagcggc tccctttgga ccaccgcgct gagattccca ctcgtcccca acaaccaccc
     6121 gcgcgcatga aaccccctcg ccaccgccgc cagccccacc gcaatctcat taaacccatc
     6181 ggacatgcag gaaagatgct accaaacacc caaattgcgc aagtcgtctg aacagagcgt
     6241 tcttttcgtc gatgaccaat taggcgatcc agtgtatagt gaggttgtct ttgtaggtag
     6301 aggaatggcg acacaggcag acaaacttgt aactgaaatt cgagccttgc cggatgaaga
     6361 aaagcttcgt gtgctcgatg caatcctcac ggacctggac aaaccggatc aggaaataga
     6421 tcaggtttgg gcagaggaag cacgcaagcg ttggagcagc tataaagctg gccatcttca
     6481 aacggtctcc tatgaagagt taatgcgcaa atacaatcgt tagtgaacat cagattcctc
     6541 acgctcgctc aacaagaagt ggatgaagcc gtcgcctggc ttgaagagcg cacggaagga
     6601 acgggtgtgg actttctgga tgaattggat cggattgtcc ggatcgttaa gtcttatcct
     6661 tttgcctcac cgcaagttga tcgggaaatt cgccgacgtc tatttgctcg gttcccctac
     6721 tcgctgatct acggcataga tcacaacacg atagtcgtca ttgctgtagc acactctcgt
     6781 cgcgcaccgc actactgggc tgagcgtttg aagttctaat gcagaagatc cggcctgcct
     6841 gattcgtgtg aaattacgct tgcctttatt cgcagatgag agtaggatgc gttcgcttac
     6901 gatgaccacc gcttcgcaag cgttcaccaa aatcattgat aacagaccaa gcttgagtgg
     6961 cctggtgggg ggccaggtgt gagtccgcgg aagcgtggtg cctgaggggg cttctgcgga
     7021 ctcggggggt tctgagacca agaggttcgg gatctctggt ctcagagagt ggccttggtg
     7081 agggggccac gagagttcgc ggaagcgata acgcccagaa cgcttccgcg gactcgcaac
     7141 gtcacaatca atccgtgtaa tctgtggcta aaaactgatt acacagatta tggaactccc
     7201 agttaacccg ccgattctcc cgatgctcgc aaaacgcgtc agcgaactgc ccaaagccgg
     7261 cgcctggatc ttcgaaccca aatgggatgg ctttcgcgca ctcatctttc gcgagggtaa
     7321 agaagtgttc atccaaagtc gcgatgagaa gccgctgaat cgttatttcc ctgaactgat
     7381 cgatccgata ctgaagcaac tcccaacgca ttgtgtgctc gacggcgaga tcgtgatcgc
     7441 gcagaaaggc gcactcgatt tcgactcgct tcagttgcgt attcatccag cggcgtcgcg
     7501 cgtcaaatta ctctcagaac agctccccgc ctcgatcgtg tttttcgatc tgctgagtga
     7561 agggaagaag gatttgcgca atcagccgtt cgaagaacgt cgcgaacgtt tgcagtcaat
     7621 gcttgaagct gcgaagccgc cgctgcacct cacgcccgcg accaccgatt tcgacgtggc
     7681 gtccgactgg tttcgacgtt tcgagggcgc gggactcgat ggcgtagtgg cgaagccggt
     7741 tgcgggaacc tacgaatcga acaagcgagt gatgctgaag atcaaacacg agcgcgactg
     7801 tgattgcgtc gtggccgggt ttcgctggca caaaaaaggc gaccgcgatc ggattggatc
     7861 attgctgctg ggactcttta acgactcagg caacttgcag cacgttggtg tgtgcgcgag
     7921 ttttaccgat cagaagcgca tcgagttagt tgagtttctg aagccttacc ggaaaaacgc
     7981 gctcgagaat catccgtgga aagagtgggc tggtgccgat cttgcgaaac ggatacccgg
     8041 cgcacagagt cgctggagcg ctgggaagga tttgtcgtgg gaacctctgc gagttgagct
     8101 tgtggttgag gttgcttacg agcacatgca gggtgatcgc ttcaggcaca cggcgcagtt
     8161 tcgaaggtgg cggactgata agaagccgag cgactgcacg tatgagcagt tggaagtcgt
     8221 accgccgcat gaaatcgagc tgatcttcaa gacaaaatct tagacaggat gacaggattt
     8281 acagaattga caggattcaa tcttgaaaat tttgttaatc ctgtgaacgt ctgcggtgca
     8341 tttcacgcca ctcgcgcgtg gggtcgtcgt cggggtcggg cgtctcctgc gcgggccgga
     8401 ttttttcagg aacgttgcgc aggttaactc tgatacgcgt ccacgttgat gaacgacctc
     8461 gcatcgtatc gaccagcaca tcatctacag ccagcagctt cgacactttc gaatacttct
     8521 tattccaacg ttcaagtccc tcgagcgcgg ccttcttatc aggcgagttg gccaccacga
     8581 tcagtggcat gcgcacgcgc ttcttcgccg ctggtttttt cgcccgcgaa ggcgcgacac
     8641 gcggcgcctc gccttccatt ttcgcgaaat gcggcggcca cggcgcatca cccaaaccgg
     8701 acgcctcgtc ccggtctgca agctcgagca gtttctccag cgaaccagcc gcagcatcca
     8761 tctccgcgtg cggattcccc agatcagcaa aacgcttcgg aacggtcagc accgtaaaat
     8821 cagccggctc acatccctcg acttcattcc attcaagcgg tgtcgaaaca cgcgcgtcgg
     8881 gaagtggccg cacggaatac gccgaacacg tggtgcgatc tttcgcgttc tggttgtagt
     8941 cgagaaacac gccgtgtcgc tcttctttcc accacttcga gctcgccagc ctggggacgc
     9001 gccgctcgat ctcacgcgat aacgccaccg ccgctcgtct tacttcgctg aacgtccagc
     9061 gcggctcgat gcgcacattc acatgcatgc cgcgcgagcc actcgtcttg ggccaaccac
     9121 gcaaacccat ctcctccagc aacgctttca cttcaagcgc aaccagtcgc acactctccc
     9181 actccacgcc tggtccagga tcgagatcga cacgcaactc gtcaggatga tcgagatctt
     9241 tcgaacgcac cggatgcggg tgaagctcga tacaaccgag attgacgacc caaaccagcc
     9301 ccgcagcatc atcgacaacc acttcgtccg ccgtgcgtcc ggacggaaac gacaacgtga
     9361 cggtgcgcag ccactcggga tgattatccg gcgcgcgctt ctggtaaaac acctgaccct
     9421 cagcgccgtc gacaaagcgt ttcagcacga tgggacgatc gcgaatcccg gcgagcgcgc
     9481 ccggtgcgat cgataagtaa tattggacaa gctcgagttt cgtgagcttc gtttgcagtg
     9541 agaaatacgg cttatcggga ttcgtgacgc ggacctcgcg accatcgatc gtcagtattt
     9601 cttccttaga cttggccacg gctcacaaag atttcgacat gttaataaag tggcaaggga
     9661 tttttgttgg gacatccggc ggaaacggag tcgtaccgtc agcgacatac ccgcgccttt
     9721 gatagaagga cggaagatcc tcacgaagac tgacgataag gatgtccatg ccgcgtgatc
     9781 cgcgctcgcg acaatatttt tcagcctcca acatgagcct tgaaccgagc ccgccttgct
     9841 ggcatgttgg atcaaccgac aacagtccta aataagatcg cgcaccgcga agctcgacgt
     9901 acacgcagcc attcaacttg ccattcatct ccgcgaggag gaaagcgcct tttgcgagtg
     9961 actcctcaac ctcagcttgg gtaatgcgat tgccgtcgat gaaaaactcc tcagcgatac
    10021 gaaaggctgc gttgatgact gcagtgattt gcggcgcatc aacggtggtg gcaatgcgaa
    10081 tcatcatgac aaaagctgag ttggtttgat cacgaaatca gtctttacaa gattacagga
    10141 ttgacaagat ttacatgtta atcctgaaaa tcctgtaatc ctgtaaaaaa ctaaccttga
    10201 ttaaagaaac ccacaagtgc attatgctgc acacctccta actatgacca caaaaaccgt
    10261 aactgaaaca ccacccgaag ctcgctccgg actggatccg tggcggattg acgtgctccc
    10321 gcaggcgccg agaccgcacg gcctcggatg gttcagcgcg attggcccag gcgtgattgc
    10381 gctcggcgtc tcgatcggca gcggtgaatt cctgcttgga ccagcggcgt tcgtgaaata
    10441 tggtctgtcg ctgctgtggg tcgtcggcgt cgcggcgttg cttcagacac tgctcaacgt
    10501 cgagctgatg cgttacacga tggccaccgg cgagcctatc ttcacgggct tcatgcgcac
    10561 gcgtccacac gcgacgttct gggcgtggtt ctacgccgca ctttactttc ttcagatggg
    10621 ctggcccggt tgggccggcg ctgccgcggg tgccgtcttc tttctcttcg cgaaacgttt
    10681 gcccgtggcg agtgaagccg gaatggtcta cggcatcggt gtcgcgctgt ttcttctgtg
    10741 cgcattaatc ttgacgatcg gccgtcgtat cgagcgcaca ctcgagtatc tcaactggat
    10801 catggtctca gtgatcatcg gtggactcgg tatcctcgct gttctttttg ttccgttatc
    10861 gacgtggctc gcagcggtgg cgggctttgg cggctttgat gtgaagacgg gatcgttcca
    10921 actcgtaccc gccggtgctg atttctttct gctcggcgcg ttcgcggctt atgcgggtgg
    10981 cggtggcgtc gcgaacctga cgttgagtaa ctgggcgcgc gacaaaggct acggcatgag
    11041 cagcatcgtg ggtttcattc ccgcggcggt tggcggcaag aaagtcgcac tcgcccactc
    11101 gggtagcacg ttcaaaccgg atgctgaatc gcttcaacgc tggcgtggct ggtggcgcat
    11161 catacgtgtg gaccagtggg gcgtttactt cacgggcgcg attctcggca tgatgttgcc
    11221 gggactgctt tacgttacgt ttgtgcctgc gggcagcgat attcgcgggc tcggtatcgc
    11281 agcagcgctg gcgcaggcag tggcgacaca gaaaggcgcg atcttcggcg gcgtgatcgc
    11341 ggctctcggc gtctggttac tgttcaagac acagctcgat attctcgaag cgatgacgcg
    11401 tggagtcaca gacattctct gggcgggcag tgcgcgcatt cgtgcctggc gcgggggcga
    11461 cgtgcgcgtg gtttattact cggtgctcgc ggtgatcacg gtctggggga ttttggcgtt
    11521 gcgtttgacg cagccgatcg tgttgctggc gttgagcgca aacatggcgg ggatcgtttt
    11581 tatcatcagc tcgttgcact tgctttatgt gaacacgcgt ttgttgccgg ttgagctgag
    11641 gccgccgtgg tggcgacggt tagcgttggt ggcgctgtcg ttgttttacg gaatgttcct
    11701 gatgctgtgg ttgtggagct tgatcatgaa gtaagcttag ccacagatta cacggatttc
    11761 atacacagat ttaccaggat gacatgcatt ataaattcct cgtactcgct ttactgctcg
    11821 tagtctcacg cgtatcagca caggagaaac cgcagacgtt caccgcacag ggaatctcgg
    11881 ttgagttcac tgccacgcac cctgtcgccg gtgaagagat caaggttcaa tttaagatca
    11941 cggacacgaa tggcgcggct ttgagcagcc tgcgaccagt cgcgtggatc gatcagcgcc
    12001 agccgaacca gcctctcgac gcgcgtgttt gtcgtgaaaa ggtgcaggcg tttttgcagc
    12061 cgagtttcag cagccgtccc acgctcgatc tcaacgcata cttcattctg gcgttgaata
    12121 acgaacccaa tatctcagtg atcgatccgc tgtcaggctt tggtggcagt aagctctaca
    12181 cgctcatccc gctgaagagt cccggtgaag actgggtgct ggctgacgac aacaagcgtc
    12241 tttacgtctc gatgccgcaa gtcaaccagg tcgccgtgat cgatatcccg acgtggaaag
    12301 tgatcgcgaa tgtcgatgcc ggagcgatgc ccacgcgtgt cgctttacaa cgcgacgagc
    12361 gatacctgtg ggtgggcaat gatagcgagg ccgccagcgg cgtaaccgtg atcgatacga
    12421 cgacaaacaa agtcgcagcg caaataaaaa ccggactcgg ccatcacgag atcgctttca
    12481 ccgacgacga tcgactagcc ttcgttacca acaagcagac cggaaccctc tcactcatcg
    12541 acatccgaaa gctaacaatc ctgaaagatc taaaagtcgg ctcacaaccg gcagcaattg
    12601 ctttctcgac cttgagccag actgcgtacg tcgcaaacga gggcgacgga acgatcgccg
    12661 caatcagcgc ggggaaacaa gatgtcgtgt cgcgcatgcc gaccgcgccc ggtctaactg
    12721 cgatcagtat tccttcgaac ggccactatg gcctggctct caacactgcc aaaagcacgg
    12781 cttacgcttt cgatctgtct tcgaatcgcg tggtacagac cgttccagtc ggacccggct
    12841 cggatcagtt gagctttacg aaacagtttg cttacgtgcg tgcgtcgggc agcgagttcg
    12901 tcacgatgat caatatggcg aacatcggca aggaagcgaa cgtcacgagg tttcccgcag
    12961 gacagcgcgc gcccggtgaa tcgctctcgc gctcgcacgc cgcggcaatc gtcccggcgc
    13021 cggaagaagg cgcggtgctc gtcgccaatc ctgccgacaa gatgatctat tactacaccg
    13081 aaggcatggc cgcgccgatg ggaagttttc aaaactacaa gcgcgatccg cgagcactgc
    13141 tggtgatgga caacagcctg cgcgaaaccg cgcgcggtat ttactcaacg acagcacgac
    13201 tgaacaatcc cggttcatac gacgtcgcgt tcctgctcga ctcgccacgc ctcgtcaact
    13261 gtttcgaaat ccaggtggcg cagaacccga acgcgccaac gaaagttcag agcgcataca
    13321 agatcgagcc ggtcgtgaaa gaagcggtgg cgcgcccggg cgaacgcttc accctgcact
    13381 tcaaagtggt cgactataaa accggagccg cgaaagccgg actgaacgat ctcagcgttc
    13441 tcgtgtttct cgcgcccggc atctggcagc aacgcgacct cgcaaaatcg tcgacagatg
    13501 gcgtgtacga aacgagtttt gttccgccga gtgagggcgt gtactatgtg tacttccaaa
    13561 gcccctcgct tggaatgcaa tacaatcagt caatgccgct gacgattcag gcagcgaagc
    13621 cgtaacagtc tccccaaacg aattctttca acgaggtctc acatgaaatc aattcagcat
    13681 tcatacacgt gggcgatgtt gacgcttgtt ttgctgatcg cggctactgt tcacgcgcag
    13741 gaaccaacgg cgcctccgca aacagtcgtg cctaacacgc aggaaaataa cgcacagaga
    13801 tatttcactg acaccgtcct catcaatcaa aatggcgagc gcgtgcgctt ctacacggat
    13861 ttgatgaaag acaaaactgt catcatcgat acatttttcg cgacgtgcca ggcgagctgt
    13921 ctgccgatga accgcaatct cgcgcagatt caggaaggac tcggcgatcg tctcggcaag
    13981 gacgtgaaca tactttcgat cagcgtcgat ccgggtgtcg acacgccgac gctattgaag
    14041 gcgtatgcta agaaactcaa cgcgaagccg ggttggtatt ttctgacggg cgacaaagag
    14101 agtattgagt tcattttgaa aaagttgggc cagtttgttg atgacaagaa cgatcatctc
    14161 aacatcttcc tcatcggcaa caatcgaacg gggctttgga agaaagcgtt cgggctcgcg
    14221 aagagtgaag agttgatgaa ggttgtcgag agtgtggtca acgaccccgg caccgcagcc
    14281 aagccttagc caggattaac aagattcgac aggatttaca tgtaaatcat gaaaaatctt
    14341 gcaaatcttg tctagaactg acgttgaaaa tcatgcttct aaaagtaatc gcgctcgctt
    14401 tactcgtctt tatcccgatc ccgggcgtca gcaacgctca atcaactcgc aagcccgcgg
    14461 caaagcgtgt tgtttacgcg tgcccgatgc atccggacgt gacgtcgtcc aaacccgggc
    14521 gctgtccgaa gtgcaagatg cagttgcgtc cggtgaaacc agagccatcg cctaggcctt
    14581 ctccaacacc gaacccaact ctctcgttca gctcggcgaa gatcccaaac acgcgcatcc
    14641 tcgatcagaa tggcaagcag ctcaacttct acaacgatct aatcaagggc aagacggttg
    14701 ctattaactt catcttcacg acttgcacca ccgtttgccc gccgctgacc gcgactttcc
    14761 gcaaggtgca acagacggca acggaacgcg ggttagatgt gaagctgatt tccgtcagtg
    14821 tcgatccggt gattgacacg cctgagcgcc tgcgtgcgtt tgcggaaaag ttcaatgccg
    14881 atcaggggtg gacgtttgtt acgggagaaa aaggtgacat tgattcgtta ctccagtcgt
    14941 tgggcgtcgc ggtcacaaat aaaaacgatc acacacccat gatcctgatc ggtaacgatt
    15001 cgcttgatta ttggacgcgg gcttacggcc tctcatcacc cacaaagctc gtcgatctga
    15061 tcgccgaagc ggcgaggagt gtccgataag cttcagcttg tcggccgaca agctaaagca
    15121 tgtcggacac tcctaaaaca gtcggtcgtg gatttgtctg acgatttccg gtacgcgctc
    15181 gcgttccaca gtggcgacca ggttagatgg cgctgtgctc ttccactcga gcgtcggatc
    15241 aacttgtcgg accgccgccg cgctggcact gccgttattc aacccgtctc ccacacaacc
    15301 aacaagcgcg tgtgtcgcat tcatccggtg tgcgatcgct ttgacgacgc ccgcggacgg
    15361 ctcggacacg gcgcagataa gcgtcccatc tcgctcgggc gctcttgaat tgcgaatgct
    15421 gactggaatc cgttgcggca cgacaggctc aatcatcttc gggtgcatca cccgtgcgcc
    15481 cagcaccgcc agctgcgcgg cttcgtcgta agagatgatc gggacggtcc gcgtccctgg
    15541 cacgagcttc ggatccgcag tctgaacgcc atcaacatcc gtccagatct
//
LOCUS       DASSFQ010000307         6170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1775275, whole genome shotgun sequence.
ACCESSION   DASSFQ010000307 DASSFQ010000000
VERSION     DASSFQ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775275"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..2204
                     /locus_tag="VFH96_11040"
     CDS             147..2204
                     /locus_tag="VFH96_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET6974553.1"
                     /translation="MNRIKLIYPGVMAGFAVLALALASNAQQPAPQQAASRFDVTNYR
                     IEAQIIPDQHMLRAGADVTFVPQDATRSVVFELNGSLKVESIEMAGKPLTGYVQDAVG
                     VGSLGPSVRVDLGSVMPANQPITLRIRWSGALVSPEGGPLATKRLAYVGTEGSYLMYA
                     SRWFPFHDYAADRATSDITFIVPAGLQVAGTSDDAIVAQPSPKEGAVRYHFVHRQPVL
                     IGNFAAGQYINKNLRYANYDLNLYAMPGSEKRLEQYAELTGRVLEFYTKKYGKPLFGN
                     RLVVVQIDDESLDTYSGPGIIYLSSKMFDTNRPVPEEKIEREIAYQWWGQSVGLKSFD
                     DAWLSQGLAEWSAFAYRETTLTAGGLDAAQREQQERALTFEQTASIARAPSALDDQSA
                     AYQSIVFYKGSMIFRMLRETIGKEDFDRTLAAFLDSYKGKNASIDDFEKLAAKISQQN
                     LRYFFAQWIEGTGVPEFTVDYQIIRTRSGKFRTRGTVKQNLETLRMPVELLLRAEGDN
                     QQKIVRIEGRSEDFDFESNGQPIEVVVDPNNKILRMSDDLRVSIIARRGIEQMKEGIY
                     AEAQQQFEAALKLDRSNSWVYYNLGLLFLEQRNWQQALDNLEASLIGNLKPSWIEVWA
                     HIKRGNAYDAKGERDRAVYEYNKAVQSGIDYDNAQAIAKKYLAVPYDPKAEQTAEANP
                     QGQ"
     gene            complement(2249..3058)
                     /gene="lpxI"
                     /locus_tag="VFH96_11045"
     CDS             complement(2249..3058)
                     /gene="lpxI"
                     /locus_tag="VFH96_11045"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET6974554.1"
                     /translation="MKFGLIAGNGKFPFMVVEGARRSGVPLVVAAIREETDPEMDQRA
                     DRVKWVGIGQLGRMIRFFKEEGVEKAIMAGQVKHVQIFSRAVPDARMLRVLLKLPRRN
                     TDSLIGAIASELESEGIELVDSTCFLQDSLPQNGTLTRREPSKQEREDINYGLEIAHE
                     IARLDLGQTIVVRGQACVAIEAMEGTDETIRRAGRLAKGRLTVVKVAKPNQDMRFDVP
                     VVGVPTIEAMIEAGATCLCITAGKTLMFDRDQMIRTAERSKISILAADLHG"
     gene            complement(3055..3849)
                     /gene="lpxA"
                     /locus_tag="VFH96_11050"
     CDS             complement(3055..3849)
                     /gene="lpxA"
                     /locus_tag="VFH96_11050"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET6974555.1"
                     /translation="MSTATHPTSIHPTAVVSPGARIGDDCFVGPYCIVGDEVQLGEGV
                     RLESHCVVDGRTSIGAGTHVFPFVSIGLASQDLKYKGEPSETIIGERNKIREFVTIHR
                     GTAGGGMLTKIGDDCLIMAQAHIAHDCLIGSGVIMANAATLAGHVVIEDRANVGAYSG
                     VHQFCRVGKEAYIGGYSVVVKDALPFALTVGNHAKCYGLNITGMKRRGYSRATIDSLH
                     HAFHLLLSAKLNTTQAVERIGQEIAGSPEVSDLIRFIEASDRGVTK"
     gene            complement(3883..4332)
                     /gene="fabZ"
                     /locus_tag="VFH96_11055"
     CDS             complement(3883..4332)
                     /gene="fabZ"
                     /locus_tag="VFH96_11055"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET6974556.1"
                     /translation="METVLDAQAIQQLLPHRYPFLLVDRIVELVPRERIVGIKQVSIN
                     EYFFQGHFPGAPVMPGVLVVEALAQVGAVLALREIEDRDQKLVLFTGIKEARFRRPVV
                     PGDTLTLEVTALRIGSRVQRMRGEAKVDGQLAADAEIMSIVAPREEK"
     gene            complement(4398..5042)
                     /locus_tag="VFH96_11060"
     CDS             complement(4398..5042)
                     /locus_tag="VFH96_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET6974557.1"
                     /translation="MRRHLFPVSVLLVAAFVASISAQQPAGRPAAQPTPTAAPAATVQ
                     LPTSKMAVIYTDLFLDQKTGIAKFTNLVTKLNGEFQKPKDDLTQMQTRASTLESEITK
                     LREAPEGTPIDQRSLQAKIDQLDQLKKDIQRKAEDAQGSYNRRRQELFLPLQEEIGKA
                     LEAFAKARGINVIIDGTQVPLLYAADSIDITRAFIADFNSKNPATAAITTTPPK"
     gene            complement(5086..5697)
                     /locus_tag="VFH96_11065"
     CDS             complement(5086..5697)
                     /locus_tag="VFH96_11065"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET6974558.1"
                     /translation="MKIARFFAAVAMLTVAAFCVSAQTKPATTAAPQAAAPQNVAVPD
                     SKMAMIYSDAFLDPKTGIARFNSLLTTLNREFQPRQAELQALQTKINTLTKEIDDTQT
                     VADPNAIRTKRDQLAQMTTEYKRKGEDAEAAYGKRRQDIFLPLQQDIGKALEAFAKAR
                     GITVIVDGSQVPVVYAADNIDITRAFIAEFNSKNPATASVTQP"
     gene            complement(5784..>6170)
                     /locus_tag="VFH96_11070"
     CDS             complement(5784..>6170)
                     /locus_tag="VFH96_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974559.1"
                     /translation="LLAGTPGLTSLVARDNRIVSQAELEAAQRLDPPDPFTGLPAGFQ
                     PVFLRGQAQTNSVVRLSQSLFDSIGDYRSSLGMEVRVQVPVINVPFRLIFAYNPNARP
                     NQFIDGFPFFFNEKKRVIRFSVGRTF"
BASE COUNT         1357 a         1726 c         1720 g         1367 t
ORIGIN      
        1 tcatctggcg cctccgacga agtagcgtga ctgactacaa ctgtaggggc ggccctccgt
       61 ggccgcccgt tcgtcgattc tcagccaacc gcttctggct tcttaccatc caaagtcctc
      121 gactgtataa tcgtcgcaat aaagccatga atagaatcaa gctcatctac cccggcgtta
      181 tggcgggttt tgccgtcctt gcgctggccc tcgcaagcaa cgctcagcag cccgcgcctc
      241 agcaagccgc cagtcgtttc gatgtgacga actaccggat tgaagcgcag atcattcccg
      301 atcagcacat gcttcgggcc ggcgctgacg tgacatttgt gccacaggac gctacgcgct
      361 cggttgtttt cgagctcaac ggctcgctga aagttgaaag catcgagatg gccggcaagc
      421 cattgacggg ctacgttcag gacgcagtcg gagtcggctc gctcggcccg agcgtccgtg
      481 tcgacctcgg cagcgtaatg cctgcaaatc agccgatcac actgcgaatt cgctggagcg
      541 gcgcactcgt ttcgccggaa ggcgggccgc tcgcgaccaa acgactggct tacgtcggca
      601 ccgagggttc gtacctgatg tatgcctcgc gctggtttcc gtttcatgac tatgccgccg
      661 accgcgcaac ctcggacatc acgtttattg tgccggcagg tttgcaggtg gcaggcacga
      721 gcgacgacgc aatcgtggcg caaccgtcgc cgaaagaagg cgcggttcgc tatcacttcg
      781 tacaccggca gccggtgttg atcggcaatt tcgccgccgg gcaatacatc aacaagaacc
      841 tgcgctacgc gaattacgat cttaatttat atgccatgcc gggcagtgaa aaacggctgg
      901 aacaatacgc cgagctgacc ggacgcgtgc tcgagttcta cacgaaaaaa tatggcaagc
      961 cgctttttgg caatcgttta gtggtggtcc aaatcgacga tgagagcctc gacacttatt
     1021 ccggaccggg aatcatctat ctgtcgagca agatgttcga tacgaatcga cccgtgcccg
     1081 aagagaagat tgagcgagag attgcttacc agtggtgggg ccagtcagtc ggtctgaaat
     1141 cgtttgacga tgcgtggctt tcgcagggac ttgccgagtg gagcgcgttc gcctatcgcg
     1201 agactacgtt gactgcaggc ggtctcgatg cagctcagcg tgagcaacag gaacgtgccc
     1261 tcacgttcga acaaacggcc tcgatcgcga gggcgccgag tgctctcgac gatcagtcgg
     1321 cagcgtacca gtcgatcgtc ttctacaaag gctcgatgat cttccgcatg ttgcgcgaaa
     1381 cgatcggcaa agaagatttc gatcggacac tcgctgcgtt cctcgactct tacaaaggca
     1441 agaacgcgtc gatcgacgac tttgaaaaac tcgcggccaa gatctctcag caaaacttac
     1501 gctacttctt cgcgcagtgg attgaaggaa cgggtgttcc tgagtttacg gtcgattatc
     1561 aaatcattcg cacccgttca ggcaagttcc gcacgcgcgg cacagtgaaa cagaacctcg
     1621 aaacgctgcg catgccggtc gagttgctgt tacgcgcgga aggcgacaac cagcagaaga
     1681 tcgttcggat tgaagggcgc agtgaggatt tcgactttga atcgaatggg cagccgattg
     1741 aggtggtggt cgatccgaac aacaagatac tgcgaatgtc agacgacctg cgtgtttcga
     1801 ttatcgcgcg acgcggaatc gagcagatga aggaaggcat ctacgccgag gcgcagcagc
     1861 agtttgaagc ggcgctgaaa ctcgatcgct cgaattcgtg ggtttactac aatctcggct
     1921 tgctgttttt ggaacagcgc aactggcaac aggcgctcga caatctagag gcttcgctca
     1981 ttgggaatct gaagccgtcg tggatcgaag tgtgggccca catcaaacgt ggcaatgctt
     2041 acgatgcgaa gggcgagcgc gatcgcgctg tgtatgagta caacaaggcg gtgcagtccg
     2101 gaatcgatta cgacaacgct caggcaatcg cgaagaaata cctcgccgtc ccctacgatc
     2161 cgaaggccga gcaaaccgcg gaagccaatc cacaaggaca ataatttagc cgcggataaa
     2221 cgccgatgaa cgcggatctg atccgcgttt atccgtgtaa atccgcggct aaaatactga
     2281 tcttactcct ctcagcggta cggatcattt gatcccgatc gaacatcaag gtctttcctg
     2341 cggtaatgca taagcaggtg gcgcccgctt caatcatcgc ctcgattgtc ggtactccaa
     2401 ccaccggcac atcgaagcgc atgtcctgat tcggcttcgc caccttcacc accgtcaatc
     2461 gacctttagc caaccgtcca gcgcgccgaa tagtttcgtc ggttccttcc atcgcttcga
     2521 tcgccacgca cgcctgacca cgaactacaa tcgtctgacc caggtccaac cgcgcaatct
     2581 catgcgcgat ctccaatccg taattgatat cttcgcgttc ctgtttcgac ggctcgcgac
     2641 gcgtcaacgt tccgttctgc ggtaacgaat cctgaagaaa acaagtcgag tcaaccagct
     2701 cgattccctc gctttcgagc tcggaagcga tcgcgccgat caacgagtca gtgttgcgtc
     2761 gcggcagctt gagtagcact cgcaacatgc gcgcatcagg gacggcgcgg ctgaagatct
     2821 gaacgtgctt gacctgcccg gccatgatcg ccttctccac accttcttcc ttgaagaacc
     2881 ggatcatgcg gccgagctgg ccgataccaa cccacttcac gcggtcggca cgctggtcca
     2941 tctccggatc agtctcttca cggatcgcag ctacaacgag cggcacacct gatcggcgtg
     3001 cgccctcgac gaccatgaag ggaaacttgc cgttgccggc gatcaatccg aacttcattt
     3061 ggtgacgccg cgatcggaag cttcgatgaa acgaattaga tcggagactt cgggagagcc
     3121 ggcaatttcc tgcccgatac gttcgactgc ttgagtggtg ttgagctttg cggaaagcag
     3181 caggtgaaat gcgtgatgga gcgaatctat cgtcgcgcgc gagtaaccac ggcgcttcat
     3241 gcccgtgatg tttagtccgt aacacttcgc gtgattaccg acagtgagcg cgaaaggcag
     3301 cgcatctttt acaacgaccg agtagccgcc gatgtaagcc tctttgccga cacgacaaaa
     3361 ttgatgcacg ccggaatatg cgccgacatt cgcgcgatct tcgatcacga cgtgaccagc
     3421 cagcgtggcc gcgttggcca tgatgactcc gctgccgatc aggcaatcgt gggcaatatg
     3481 ggcctgggcc atgatcagac aatcgtcgcc gattttggta agcatgccgc cgccggcagt
     3541 gccacgatga atggtgacga attcgcggat cttgttgcgc tcgccgatga ttgtttctga
     3601 cggctcgcct ttgtacttga gatcctgtga ggcaagaccg attgagacga atggaaagac
     3661 gtgtgtgccg gccccgattg aagtgcggcc atcgacgacg caatgtgatt cgagtctgac
     3721 gccttcgccg agctgtactt catcgccgac gatgcagtat ggaccaacaa aacaatcgtc
     3781 gccgattcgc gcgcccgggc tcaccactgc agtgggatga atgctcgtcg ggtgagtggc
     3841 tgtagacata aaatataagt cctataggac cgataggacc tattacttct cttctcttgg
     3901 ggcgacgata ctcataatct cagcatcagc ggcgagctgg ccgtcgacct tggcctcacc
     3961 acgcatgcgc tgtacgcgcg agccgatccg caaagcagtt acctcgagcg tcaaagtgtc
     4021 gcccggaacg accggccgtc gaaaacgtgc ttctttgata cccgtaaaca gaacgagttt
     4081 ctgatcgcga tcctcgattt cacgcaacgc cagcacggcg cccacctggg ccaacgcttc
     4141 gacaaccagc accccgggca tcaccggggc gccgggaaag tggccctgaa aaaaatactc
     4201 gttgatactc acctgcttga tgccgacgat tctttcgcga gggacgagct ctacgattcg
     4261 atcgacgagg agaaacggat agcggtgcgg caggagctgt tgaatggcct gtgcgtcgag
     4321 aactgtttcc atttagcaat caaggccgca aaaaggcaca aaaacgagtg tttgtacttc
     4381 ttgtgctttt ttgtggccta tttaggcggc gtagtagtta tcgctgcggt tgccgggttc
     4441 ttgctgttga agtcggcaat aaacgcgcgc gtgatgtcga tactgtccgc cgcgtacagt
     4501 aaaggcactt gcgttccgtc gatgatcacg ttgatgccac gcgctttggc aaaggcttcg
     4561 agcgctttgc ctatctcttc ctgcaacggc agaaagagct cctggcgacg acgattgtaa
     4621 gagccctgag catcttcggc tttacgctgg atgtcttttt tcaactggtc caattgatcg
     4681 atcttggctt gcagcgagcg ttgatcgatc ggtgttcctt ccggcgcttc gcggagcttg
     4741 gtgatttcag actcaagcgt cgatgcgcgc gtctgcatct gcgtgagatc gtccttgggc
     4801 ttttgaaact cgccgttcag ctttgtcacc aggttggtga atttcgcgat ccctgttttt
     4861 tgatcgagaa agagatccgt gtagatgacg gccatcttgc tcgtcggcaa ctgcacggtg
     4921 gccgccggag cagcagtcgg agtgggctga gcagcaggac gccccgcggg ctgttgtgcg
     4981 ctgatggacg caacaaaagc cgcgacgagc aacacgctga ctggaaacag atgacgtctc
     5041 atgatttttt taataggtcc tacaggactt ataggaccta ttaagttacg gctgcgttac
     5101 tgacgcagtt gccgggttct tgctgttgaa ctcggcgatg aaggcgcgag tgatatcgat
     5161 gttatcggcc gcataaacca ccggcacctg gctgccatca acaatcacag tgataccgcg
     5221 cgctttggca aacgcttcaa gcgctttacc gatgtcctgc tgcaacggca aaaagatatc
     5281 ctggcggcgc tttccataag ccgcttcggc atcctcgccc ttgcgcttgt actcggtggt
     5341 catctgcgcg agctgatctc tcttggtccg gatggcattc ggatccgcga cggtctgagt
     5401 atcgtcaatc tctttggtca gcgtgttgat cttggtttgc agggcttgca gctcagcctg
     5461 gcgcggctgg aattcacgat tcagagtggt tagcaaacta ttgaatcggg caatcccggt
     5521 tttcggatcg agaaacgcgt ccgaatagat cattgccatc ttgctatccg ggacggccac
     5581 gttctgtggc gccgccgctt gtggagctgc ggtcgtcgcg ggcttcgttt gtgctgacac
     5641 gcagaatgca gcaaccgtca gcatggcaac tgcagcgaaa aagcgagcta tcttcatgac
     5701 tttcttgtgg tactccttaa gttgagggcg attggacaaa gcctttagtt gttcatccag
     5761 ccaccatggg ttggctcggg cgatcagaaa gtgcgcccga cactaaagcg tatgactctc
     5821 tttttttcgt taaagaagaa cggaaacccg tcaatgaatt ggtttggccg ggcatttgga
     5881 ttatacgcaa agattaaacg aaacggtaca ttgatgacgg ggacctggac tcggacttcc
     5941 atacccagac tcgatctgta atcgccgatg ctgtcgaaca gcgactgcga cagcctcacg
     6001 acgctatttg tctgcgcctg accacgaagg aagaccggtt ggaacccagc cggcagccct
     6061 gtaaatggat cgggcggatc aagcctctgt gcggcctcaa gctcggcctg cgatacgatg
     6121 cgattatcac gcgcgaccaa actcgtcagg cccggagttc ccgccagcag
//
LOCUS       DASSFQ010000308        15547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_143319, whole genome shotgun sequence.
ACCESSION   DASSFQ010000308 DASSFQ010000000
VERSION     DASSFQ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15547
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143319"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFH96_11075"
     CDS             <1..648
                     /locus_tag="VFH96_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET6974560.1"
                     /translation="IDTVAAWIDQRVITTSFAAEETLTSDTVPVNVDAVLFWMVYDPE
                     KAALEVQNYPQAVSWAAQTALRDIIGRTSLTDLLRGRERIEEELQKLIDERSNPWGVT
                     VQSVEMRDVIIPAALQDAMSREAQAAREKQARIILGEAEVEIAHSFETAAKSYRDDPT
                     ALHLRAMNMLYEGLKEKGALMLVPSSAVESMGMGGLLGAAALRQQTLTGSDEAPE"
     gene            complement(695..967)
                     /locus_tag="VFH96_11080"
     CDS             complement(695..967)
                     /locus_tag="VFH96_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974561.1"
                     /translation="MEIDDDAKEIVREIGTAINESVENSTKVAEAIERLREAGYEMEL
                     TLRLEIGLRPHASDDESAADTANLDLTEEDLQVLQRMKIRIDPEGN"
     gene            1050..2162
                     /locus_tag="VFH96_11085"
     CDS             1050..2162
                     /locus_tag="VFH96_11085"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET6974562.1"
                     /translation="MFATIGILTGGGDAPGLNAVIRAVVKTATCEYGMRVIGIEDGFE
                     GLLGATRTRMLSSGDVRGLLPRGGTILGTRNKGRFVESACGDSERDAESVYQEAISNM
                     KIVGIDALVVLGGEGTLTIANEFDKRGVPVCGVPKTIDNDLACTELTFGFVTALDIAT
                     EALDRLHTTAESHDRVMILEVMGRHAGWIALHSGIAGGADVILIPEIPFSIEAVANKI
                     RQRDLSGSNFSIIVVAEGALEIGHELVYQDKGDGHRAPRLGGIGQHLQQDLERVTGRE
                     TRFVVLGHLQRGGRPNAFDRMLATNFGSCAVRALAESKSGVMVALQAGDVVTVPLTEA
                     IKGIKTVPANGQLVRTARDTGISFGAPDEASYHKQQ"
     gene            2165..2647
                     /gene="greA"
                     /locus_tag="VFH96_11090"
     CDS             2165..2647
                     /gene="greA"
                     /locus_tag="VFH96_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET6974563.1"
                     /translation="MSQAIKEKLQGELDELESELRIHLPKEIKRALEFGDLRENSEYR
                     AALDRQNVVKARIVELRQRISEIASIDPNKVPRDKASYGSTLVLFDGEKDEEVTYRLV
                     TPEESDPAQGLISTTSPVGRSLMGKQEGDEVTVRTPAGARSFEIRQLRTLHDDLEAQQ
                     "
     gene            2743..3444
                     /locus_tag="VFH96_11095"
     CDS             2743..3444
                     /locus_tag="VFH96_11095"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6974564.1"
                     /translation="MFGASIGRAAQRIIDAIVRWLALGHINPNILTVIGVALNVGCGL
                     LFGLGWFFSAGVTLVVANLFDMLDGQVARLSGRVTRFGGFLDSSLDRLSDMVVFVGLM
                     VFYARDIESHSTLNVFLAGAGLMGSVMVSYASARAESLIPKCDVGFLRRPERVVLFII
                     GALSTHPGSSNFFANRMPAVLWVLAVGSYWTFAHRMYHTWRELTKAEAKTPNVDAEAT
                     YDRPKRAEVAISQVQ"
     gene            3481..4386
                     /locus_tag="VFH96_11100"
     CDS             3481..4386
                     /locus_tag="VFH96_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974565.1"
                     /translation="MTTCPCCGFKFHGALSSGCKQCGARAVGEPLPKPAQELPSYGRA
                     LVLAVSGSLVVMVFVSQTLIAFFQRSEGWGFWNWVAAGETAAWRLKWISIPVLFAILW
                     IGRKLYRSILHQPQRFCGIKHARRGLLASSVVTLLIALLIGITVPARMQQRRLSKEAG
                     IRAQGYAIELALTQYKIKYKTYPADLRSLLDRTPDPDGTLAAALLNVDPLTYHATSDV
                     AANATEKVRPLRGTVIRKVGFQPDVDDSPSGGLSFTNYALVLPGEDKLYATEDDWIIQ
                     DGMIMKQSESANGGVGRSVSGLLQP"
     gene            4395..4682
                     /locus_tag="VFH96_11105"
     CDS             4395..4682
                     /locus_tag="VFH96_11105"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET6974566.1"
                     /translation="MSFLDRFRKRTEDEASRIARLSKTGRMVDGKIIDAVSDNDGRIL
                     QVNYTYEIAGVQYESSQALSDLQQQRPHDYAPGKQIVVRYEPRRPANSIVV"
     gene            4799..6295
                     /locus_tag="VFH96_11110"
     CDS             4799..6295
                     /locus_tag="VFH96_11110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6974567.1"
                     /translation="MFRKVLIANRGEIAVRIIRTCRELGVRSVAIYSEPDVESLHVRM
                     ADEAVLVGPAASAQSYLKTEAIVAAAKAVNAEAIHPGYGFLAESAEFARAVSAAGLTF
                     IGPSAEAMEIMGSKTSARRAAVAAGVPIVPGTVEPLNSFDEASATAEQFGYPVMLKAS
                     AGGGGKGMRLVTDSADLRSAFETAQAEAAAAFGDSSLYLEKAVERPRHIEIQIFGDTQ
                     GNVVHLGERECSIQRRHQKVIEECPSPIDDPELRHRMGGAAVKIGRAVNYTGAGTVEF
                     LVADATHDFYFLEMNTRLQVEHPVTELATGRDVVREQFLVATGEPLSFTQEDIEWRGH
                     AIECRIYAEDPANNFFPSPGTITHLREPAGPGIRIDSGVTRLSEVSIHYDPMIAKLAV
                     WGRTRDEAIDRLKRALDEYEVTGITTTLPFFREVVRDEEFVSARLDTGFIARFNERRA
                     GREVESRQVESDLAAIAAGLSYALRKQQTAIPTVAHTNNRWKMSGRKL"
     gene            6292..6798
                     /locus_tag="VFH96_11115"
     CDS             6292..6798
                     /locus_tag="VFH96_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET6974568.1"
                     /translation="MKLKVISGEREEDLLLKLEAGRVLAEIGDRVYSLDVRETEPDGY
                     LFFLNTNVHECRVSERAGAKDTFDISVHGHSYEVTIVDPKRLRSGQNSDRHHHGVTEI
                     LAPMPGKVVRVHTEAGASVEKGAGVVVVEAMKMQNEMKSPREGVVVSINVKPGDTVNA
                     GDVLAVIE"
     gene            6893..7597
                     /locus_tag="VFH96_11120"
     CDS             6893..7597
                     /locus_tag="VFH96_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET6974569.1"
                     /translation="MRILLWDIDGTLIRSTRPGGYKVYTIPVLEEIFGTSGRLADMRV
                     SGMTDLQIVFEALSDAGISQEEILARAEVLVARLTEEARRVTGNGVKFFELLPGVRET
                     LEALAAHPRYKSALVTGNIKPMAELKMELVGLDQFFTLPGAFGEESHNRRDLPARAAK
                     RIRKHLQMDIAPEHFIVIGDTPNDIDCAHHFGARAVAVGTGRFYSTEEVLEHKPDKFL
                     PDLSDVDLVLNTLAEL"
     gene            complement(7594..8784)
                     /locus_tag="VFH96_11125"
     CDS             complement(7594..8784)
                     /locus_tag="VFH96_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974570.1"
                     /translation="MQEITADKPSVSSNGTAQTQTQLAPNLIGKILGDNTPLVIWLVF
                     FAIGGGILALYYAQIGYLPDIEWKAALIYLFVGSMVGGAVGLLLTISLFFPGVIWAEF
                     IIFDVGLSKCLAYDSQHQEPSGKESIHKEPCIRSMIIYLGAPFVAALLLSHLALWFRA
                     PINSADGVWYLDLYWFIVVLILIVTFFVMRTLFGCILSHEGWKKFWSILKTKPLDVIR
                     NKQPVLAENQNREVFKYAFWFTLAVLLNQIAMYVTYRLADRPRGGFTFFVLTALCTAA
                     VAISTHTVAFLYQEHKRKAIIAALVAAGLLLFTADNFSSLSVRLMNHYGIGYYERFNL
                     LVTKDCDDNLRTLGVPACGLQQLCNVEILSKIGDHYYLRIGDQAYVTLRKSDVVAIRP
                     LHHP"
     gene            complement(8899..10722)
                     /gene="mutL"
                     /locus_tag="VFH96_11130"
     CDS             complement(8899..10722)
                     /gene="mutL"
                     /locus_tag="VFH96_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET6974571.1"
                     /translation="MSKIRVLADHVANQIAAGEVVERPASVAKELVENSIDAGATRIT
                     IEIEAGGRRLLKISDDGEGMVRDDAILAFERHATSKIRETDDLAAIGTLGFRGEALAS
                     IASVAKVELTTCIEGANAATRVTIDGGRMRDVKDAAHPRGTTLIVRDLFFNIPARRKF
                     LRSEATETYHLTNLVTHYALAHPEIAFTFINNGREVVRTAPAKDLRERAYQLFGEEFL
                     KNLLEVDGGEREFARVSGFVSAPRDRRTSRDSQYLFVNRRFVRDRMIGRALSEAYRSI
                     LPHGVYPAALLFIETPLEEVDVNVHPAKTEVRFRRQAAVADAVREAVRAALASASYVP
                     PPEPPIMTAIAAVSSITPQPRIEFIPPPSPPPRAPVEPSGDEIARDLEIMLRSASQSV
                     KSAALPPLNSAEKIAREVTPESLSTNIRPLGQLDESFIIATDDQGLLLIDQHVAHERV
                     LFDKYRALEAERRTDSQQMLVPETFDLTPAQAAVFDDLMPELEKYGFELMRLSGRTVA
                     IRAVPADLPASEARNMLFELLETVDAEKKSNAPETMRDEIAASLACHAAIKVNMPLAP
                     EKMRWLIDRLLQTSSPTTCPHGRPVILRLSTRDILKGFHRI"
     gene            complement(10758..12443)
                     /gene="bshC"
                     /locus_tag="VFH96_11135"
     CDS             complement(10758..12443)
                     /gene="bshC"
                     /locus_tag="VFH96_11135"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HET6974572.1"
                     /translation="MSTVEAACYSTPEESGLRVESLPFDLIPHQSRLFLDYLKDPVAL
                     RNFYPSAVRFHHELQQRVPDVLSAYRVDRARVCDALEAMNQRWGAGEETLKNLALLRD
                     EDCIAVVSGQQAGLFTGPLYTVYKALSAVKLAGCLQQRGTKAVPVFWIAAEDHDFAEV
                     AKAELIGRDCQLKSVAVSEALHREGQPVGQVKLDDSISTVVDELFELLPASEFAADMK
                     ALVKSAWQPGRGYVESFASMMTALLGRYGLIFLDPLDPALKQLAAPLYSEAARRAPEI
                     ASALEQRSAELVRTGYHAQVLATANSFPLFLHDEEHRRHAVVRIENGKYRTKASEQEY
                     SAEELATLAHEKPERFSPNVTLRAVVQDYLLPTIAYYGGAAEIAYFAQTAEVYRVLDR
                     PATPILPRSSLTMIEHHTGRLLRRYDLTLADFFEGLDPVIKRVVEEHLAADSAKLFAD
                     AEQNMNHELDRLRQELQSIDPTLASALETGRKKINYQLDGLKQRFVRAQMTRDEAAHR
                     QLQRAFDQLYPNKDLQERHINITSLLARHGTYVIEWIFNAINLGSNEHQIVYL"
     gene            complement(12541..13428)
                     /locus_tag="VFH96_11140"
     CDS             complement(12541..13428)
                     /locus_tag="VFH96_11140"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET6974573.1"
                     /translation="MRALKGILLPTTTPFDPDGQISAADITSNIKEWSARGVIGFVVL
                     GSTGERVHLDEREYLEVIEVSRAATGSVLIVGAGQQSTVNTIKEIKNAARAGAEAVLV
                     ITPYFYRPAITQETLVNYYTAVAEAAPVPVLLYSMPPLTGIKIEPETVARLSEHPNII
                     GVKDSSNDVAGFRRTVELCPSDFAVMTGNGTVLLDALRAGATGGILAVGCVVPEVCVE
                     IFRAFAAGEDAEHLQEELTPLASAVTTKYGIGGLKAALEFAGYRGGAVRAPLRAPDDA
                     ARAEIKSVLDRISLMSDKL"
     gene            complement(13470..>15547)
                     /locus_tag="VFH96_11145"
     CDS             complement(13470..>15547)
                     /locus_tag="VFH96_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974574.1"
                     /translation="AGQSDKKIVETDQSVGSCDGINLETANYKVRSIRIDDPFLFLPW
                     VKARQKRASAQITELIQGKPFTYSDVSDKALKIIEDQNFLPETSDARVQFRLEFVSVQ
                     NCTDGQVDVIYRIYSTQILPVLSARPEERVKERETPQTPAGQTTALTQESSPFHFVPT
                     GGYDSTNLLFGGGRFEIKPRRIGKLPFNSALIKGEASSRMHEVSAALEGSYDSPVEHP
                     AWLAHADWLVNFSHYSLPTGAGDIRGGHVMLHFAGVTRPLAGGNMSLRFGAALDGGNR
                     QTEAGNLILAPNTIRNAGFGTLKLYGGLDSRFARNIFSASYGLELGSVGPAARVDWRK
                     HIFDARHQFWYSLGNHHLLDLESRFTMGKLQIPGKVPLPERFFGGNNEEFLIASDDWQ
                     IRANPVIRAIPGSRFFQTAEGAGGRSFVSYNLTAAYGIWRQTLVPEELTSDPEFNDQL
                     EGSITSVTSTLEVFYYSKDPHYANVVTQLPAAQTALQDLQTVVNTAQGTHAGQLEDQF
                     EACTDAIKAVSRRIRNAISTSRVPYGSIAEVLSEDPDDDQLLNVQQACEKDLNGTLKE
                     PSLTSATSRVETQRTQLTAEFNQIDTKAAGKKAKDDMAFTRRTLNTLFNDVNIYSISP
                     VFVFDMARIGPRSGAFGGTRYGPGAGIRFELATTAHFTLGYAWNVNQGPGEGRGNIFF
                     AIGVRDLFH"
BASE COUNT         3410 a         4221 c         4500 g         3416 t
ORIGIN      
        1 atcgacaccg tcgcggcatg gatcgatcag cgcgtgatca ccacgagctt cgccgctgaa
       61 gagacgctga cgtcagacac tgttccggta aacgtcgacg cagtgctctt ctggatggtc
      121 tacgatcctg agaaagcagc gctcgaagtt cagaactatc cgcaggccgt aagctgggcc
      181 gcacagacgg cgctgcgaga catcatcggc agaacatcgc tgacagatct gttgcgcggt
      241 cgcgagcgga ttgaagaaga gttgcagaaa ctgatcgacg agcgctcgaa tccatggggt
      301 gtgactgtgc aatcggtcga gatgcgcgac gtgatcattc ctgccgcgct tcaggatgcg
      361 atgtcgcgcg aggcgcaagc ggcgcgagag aaacaggcgc gcatcatcct cggcgaggct
      421 gaagtcgaga tcgcacactc gttcgagact gcggctaagt cttatcgaga cgatcccaca
      481 gccctgcacc tgagagcgat gaacatgttg tatgaaggtt tgaaggagaa aggcgctttg
      541 atgctcgtgc caagtagcgc ggtggaatcg atgggaatgg gcggcctgct tggcgccgcg
      601 gcgctgcgac aacaaacgct aaccgggagc gatgaggctc ccgaatagct aaacaacaga
      661 taggtcctat atgtcctata ggacctattt tcttttaatt tccttcgggg tcgatccgga
      721 tcttcatccg ctgcaaaacc tgcaaatctt cttcagtaag atcgaggttc gcggtgtctg
      781 cggcactctc atcgtcgctc gcgtgaggtc gcagcccgat ctcgagccgc agcgtcaatt
      841 ccatctcata acctgcttca cgcaggcgct caatcgcttc ggcaaccttc gtcgaattct
      901 ctaccgactc attgatggcg gtgccgattt cgcgaactat ctcttttgcg tcgtcatcga
      961 tttccattcg ctgttatgct acgccagtcg tcgcagattg ccaactccca atcggcaatt
     1021 cgcaatcgac aatgatagac ttagtggcaa tgttcgcaac gatcggaatc ctgactggcg
     1081 gtggtgatgc tcctggcctc aacgccgtga ttcgtgctgt ggtgaaaact gccacatgcg
     1141 agtacggcat gcgtgtgatc gggatcgaag acggcttcga aggtctgctc ggcgcgacac
     1201 ggactcggat gctttcatcg ggcgacgtgc gtggtctact gccgcgcggc ggcacgattc
     1261 tcggcacgcg caataaaggg cgattcgttg aatcagcgtg tggcgactcg gagcgcgacg
     1321 cggagagcgt ctaccaggaa gcgatctcaa acatgaagat cgtgggtatc gacgcgctcg
     1381 tggtcctggg cggtgaaggc acgctcacga tcgcaaatga gtttgataag cgcggcgttc
     1441 cggtgtgcgg tgtgcccaag acgatcgaca acgatctcgc gtgcactgag ctgacgtttg
     1501 gcttcgtcac cgcactcgac atcgcgaccg aagcactcga tcgtttgcac acgactgccg
     1561 agtcacacga tcgcgtcatg attcttgaag taatgggccg tcacgccggt tggatcgcgc
     1621 ttcactccgg catcgcgggc ggcgctgacg tgatcctgat tccggagatt cctttttcaa
     1681 tcgaagcggt cgcaaataag attcgacagc gcgatctgag tggatcaaat tttagtatca
     1741 tcgtcgtcgc ggaaggcgct ttggagatcg gtcacgagtt agtttatcaa gacaagggtg
     1801 atggacatcg tgcaccgcgc ttaggtggca taggccagca cctgcaacag gatctcgaaa
     1861 gagtcacggg ccgtgaaaca cgtttcgtcg tgctcggaca cctgcagcga ggcggacggc
     1921 ccaatgcttt cgaccggatg ctggcgacga acttcggctc gtgtgcggtc cgggcgctgg
     1981 ccgagagcaa gagtggcgta atggttgcgt tacaggccgg ggacgtggtt acggttccat
     2041 tgacagaagc gatcaaggga atcaagactg tgcctgctaa cggacagtta gttcgcacgg
     2101 ctcgtgatac aggtatttcc tttggcgctc ctgacgaggc cagttatcat aaacaacaat
     2161 aggtatgtca caagcaatta aagagaaact ccagggcgag ctcgatgagc tggaaagcga
     2221 gctgcgcatt cacttgccca aagagatcaa gcgggcgctc gagtttggcg acctgcgcga
     2281 gaattccgaa taccgcgcgg cgctcgaccg ccagaacgtc gtgaaagcgc gcatcgtcga
     2341 gttgcgacag cggatcagcg agatagcctc gatcgatccg aacaaagtgc cacgcgacaa
     2401 ggcgtcgtac ggctcgacgc tcgtgttgtt cgacggtgaa aaagacgaag aagtgaccta
     2461 tcggctcgtg acgcccgaag agagcgatcc ggcccagggg ctcatttcta ctacctcgcc
     2521 cgtgggacgg agtttgatgg gcaagcagga aggcgacgag gtaaccgtgc gtacgccagc
     2581 gggcgcacgc tcgttcgaga tcaggcagtt gcgtacgcta cacgacgatc tggaagctca
     2641 acagtagttt ttatccgcag attacacaga ttacgcagac atctgtgtaa tctgcgtaat
     2701 ctgtggacaa tacagttctg acaggagaaa cgaacctaac acatgttcgg ggcaagtatc
     2761 ggacgtgcag cgcagcgaat cattgatgcc attgttcgct ggctggctct gggtcatatc
     2821 aatcccaata ttctgaccgt catcggcgtt gctttgaacg tcggttgcgg tcttcttttt
     2881 ggtttgggct ggttcttctc tgccggcgtc acgctcgttg tcgccaatct ttttgacatg
     2941 cttgacgggc aggtggcccg tctgtcgggc cgggtcacga gatttggagg ctttctcgac
     3001 tcctcgttgg accggctgtc agacatggtg gtttttgtgg gcctgatggt cttttacgcc
     3061 cgggacatcg agtcgcactc aactcttaac gtttttcttg cgggagccgg actaatgggc
     3121 tcggttatgg taagctacgc cagcgcccgg gccgagagcc tgattcccaa gtgtgatgtg
     3181 ggctttctgc ggcgcccaga acgggttgta ctctttatta tcggggcgct cagtactcat
     3241 ccaggttcgt cgaacttctt tgccaatcgg atgcctgccg ttttatgggt gctcgcggtc
     3301 ggatcctact ggaccttcgc ccaccgcatg tatcacacgt ggcgcgaact gaccaaggca
     3361 gaagcaaaaa caccgaacgt tgacgccgaa gcaacgtatg atcggcctaa acgtgcggag
     3421 gttgcgattt cacaggtcca ataaaacata ttggtcctat tcattctttg aggtaaacgc
     3481 ttgactactt gtccgtgctg cggtttcaaa ttccacggcg ccttgagcag tggttgcaaa
     3541 caatgtggcg ctcgcgccgt tggtgagccg ctcccgaaac cagcccagga actcccttcc
     3601 tatggccgcg ctttagtact ggcagtcagc ggttcgttgg tggtcatggt ctttgtttcc
     3661 caaacgctga tcgctttctt tcagcgctca gagggttggg gattctggaa ttgggtggct
     3721 gcaggtgaga ctgccgcttg gcgcttgaag tggatctcga tcccggtcct gttcgcaatc
     3781 ctctggatcg gacgcaagct ctaccgctct atattgcacc agccgcagcg attctgtggg
     3841 atcaaacacg cgcgacgagg tctgctcgcg tcgtcagtcg tcacgctcct gatcgcgctg
     3901 ctgattggca ttacggtccc cgcacgaatg caacaacgcc ggctctcaaa agaagccggc
     3961 atccgcgcac agggctacgc tattgagctg gcgctgaccc agtacaagat caaatacaaa
     4021 acctatccag ccgatcttag atcgctgttg gaccgcactc ctgatccgga cggcacactc
     4081 gccgccgcac tcctgaatgt cgatccgctt acctaccacg cgacctcaga tgttgccgct
     4141 aacgcaactg agaaggtgcg cccgctgcgt ggtacagtca ttcgcaaagt ggggttccag
     4201 cctgacgttg acgattcacc ctccggaggt ctgtcgttca ccaactacgc gctcgtactt
     4261 ccgggcgaag ataagctcta cgcaactgaa gacgactgga tcatccagga cggaatgatc
     4321 atgaagcaat ccgagagcgc caatggcggg gtcggacgtt ctgtctcggg actcctgcaa
     4381 ccataagcga ctccatgtcg tttctcgacc gtttccgcaa acgcaccgaa gatgaagcct
     4441 cacggattgc gcgtctctcg aaaactggcc gcatggtcga tgggaagatc atcgacgccg
     4501 tttccgacaa tgatggtcga attctccagg tgaactacac ctatgagatc gcgggggttc
     4561 agtacgaatc ctcacaggcg ctcagcgatc tgcagcagca acgtccccac gattacgctc
     4621 ccggaaaaca gattgtcgtg cgttacgagc cacgccggcc cgcgaattcg atcgttgttt
     4681 agccacaaaa aggcacaaaa acacaaaata aaatgttgct catctcgcgg cttgcaggaa
     4741 gttacgtggt tggctacaac tgtaggggcg gccctccgtg gccgcccgtc gattatttat
     4801 gttccgtaaa gtattaattg ctaaccgggg cgagatcgcc gtccgcatca ttcgtacctg
     4861 tcgcgagctc ggcgtcaggt cggtggcgat ctattcggaa cctgacgtcg agagcctgca
     4921 cgtgcgcatg gctgacgagg cggttctggt tggaccggct gcgtcagccc agagctattt
     4981 gaagaccgag gcgatcgtcg cggcggcgaa agctgtcaac gctgaagcga ttcatccggg
     5041 ttacgggttt cttgccgaga gtgcggagtt tgcgcgtgcc gtcagtgcgg cggggttgac
     5101 gttcattggt ccatcagccg aagcaatgga gatcatggga tcgaagacga gcgcgcgtcg
     5161 tgcggcggtt gcagccggtg tgccgatcgt tccgggaaca gtcgagccgc tcaattcatt
     5221 tgacgaagca agcgcgactg ccgagcagtt cggttatccg gtgatgttga aagcgtctgc
     5281 cggcggcggc ggcaaaggca tgcgactcgt tacggacagc gctgatcttc gttctgcgtt
     5341 cgagactgcc caggccgagg cggccgcggc attcggtgat tcgtcactct acctggaaaa
     5401 agcagtcgag cgcccgcggc acattgaaat acagatattt ggcgacacac agggcaacgt
     5461 ggtccatctc ggcgagcgtg agtgttcgat tcagcgtcgt catcagaaag tgatcgaaga
     5521 gtgtccctca ccgatcgacg atccggagct gcggcatcgc atgggcggag cggccgtgaa
     5581 gatcgggcgc gcggtgaatt acacgggggc gggcacggtc gagtttctgg tggctgatgc
     5641 gactcacgat ttctactttc tcgagatgaa cacgcgtcta caggtcgagc atccggtgac
     5701 ggagcttgcg accggccgcg atgtcgtgcg cgaacagttt ctagttgcga ctggcgagcc
     5761 gttgtcgttc acgcaggaag atattgaatg gcgtggtcac gccatcgagt gccgcatcta
     5821 cgcggaggat ccggctaata atttctttcc gagcccggga acgatcacgc atctacgcga
     5881 gcctgccgga ccggggatca ggatcgacag tggagtcacg cgcttgtctg aagtgtcgat
     5941 tcactacgat ccgatgattg cgaagcttgc ggtttggggt cgcacccgcg acgaagcgat
     6001 cgatcggctg aagcgcgcgc tcgatgaata tgaagtgaca gggatcacga cgaccttgcc
     6061 gttctttcgt gaagtagtgc gtgatgagga gttcgtttct gcgcgactcg ataccggttt
     6121 catcgcgcgt ttcaacgagc gccgggcggg gcgtgaagtt gaatcgagac aggttgaaag
     6181 cgatctcgcg gcgatcgcgg cgggattgag ttacgcgctg cgcaaacaac aaaccgccat
     6241 tccgactgta gctcacacga acaaccgctg gaagatgtcg gggaggaagt tgtgaaactc
     6301 aaggttatct ccggcgagcg tgaagaggat ctgctgctga aactcgaagc ggggcgagtg
     6361 ttagctgaga tcggcgatcg cgtctacagt ctcgacgtgc gtgagacgga gccggatggc
     6421 tatctcttct tcctgaacac gaacgtgcat gagtgtcgcg tgagtgaacg ggccggcgcg
     6481 aaagatacat ttgatattag tgttcacggt cacagttatg aagtgacgat cgtcgatccg
     6541 aagcgtttgc gcagcgggca gaattccgat cggcatcatc acggcgttac ggagatcctc
     6601 gcgccgatgc ccggcaaggt tgtgcgtgta cacacggaag ctggcgcgag tgttgagaaa
     6661 ggcgcgggcg tggtggtagt ggaagcgatg aagatgcaga acgagatgaa gtcgccgcgg
     6721 gaaggtgtgg tcgtttcgat caacgtcaag cctggtgaca cggtgaacgc cggcgacgtc
     6781 ctcgctgtga tcgagtaggg ccgcaaaaag gcacaaaagg cacataaaag aattgatgat
     6841 ttgacccgct caatttgtgt tttccgtttt gtgccttttg tggctaaaac ttatgcgtat
     6901 tttactgtgg gatattgatg ggacgctgat caggtcgaca aggcctggcg ggtacaaggt
     6961 gtacacgatt cccgtgctcg aagagatctt cggcacgtct ggacgactcg ccgatatgcg
     7021 agtgtcaggc atgaccgact tgcagatcgt tttcgaggcg ttgagtgatg cgggtatcag
     7081 tcaggaagaa attctggcgc gcgccgaagt gctcgtggcg cgtttgacgg aagaagcgag
     7141 acgagtgact ggtaacggcg tgaagttttt cgagcttttg ccgggcgtgc gtgagacgtt
     7201 agaggcactc gcggcgcacc cgcgctacaa atcagcactc gtcaccggca acatcaaacc
     7261 aatggcggag ctgaagatgg aactggtcgg tctcgatcaa ttcttcacac tgccgggcgc
     7321 gtttggcgag gagtcgcaca atcgtcgcga tcttccggca cgcgcggcta agcgtattcg
     7381 caaacatctt cagatggaca tcgcccccga acacttcatc gtcatcggcg acacaccaaa
     7441 cgacatcgac tgcgcccatc acttcggcgc gcgggctgtc gcagttggaa ccggacgctt
     7501 ctattcgacc gaagaagttc tcgaacacaa gcctgacaaa ttcctgcctg atttgagcga
     7561 cgttgatctc gttctcaaca cactcgccga actctagggg tgatgaagcg gccttatagc
     7621 tacgacgtct gatttacgaa gcgtgacgta agcctggtcg cctattctca gatagtagtg
     7681 gtcacctatc ttcgaaagaa tttcaacatt acacaattgc tgtaaaccgc aagctggcac
     7741 gccaagagtc ctaagattgt catcgcaatc tttagtaacg aggagattga atctttcgta
     7801 gtatccgatg ccgtaatgat tcatgagtct tactgacaac gacgagaagt tatcggccgt
     7861 aaatagtagc aatccagccg ccactaacgc agcgatgatc gcctttcgct tatgttcttg
     7921 ataaagaaag gcaacggtgt gggtcgagat cgcaaccgct gccgtgcaga gggccgttaa
     7981 aacaaaaaaa gtaaatcctc ctcgtggtct atcagcaagt cgataagtca catacatcgc
     8041 gatttgattc agcaagactg ccagcgtgaa ccagaaagcg tatttgaaca cctctcgatt
     8101 ctggttttcc gctaaaaccg gttgtttatt tcgtattacg tctaatggct tggttttcag
     8161 aattgaccaa aactttttcc aaccctcgtg tgacaaaata caaccgaaga gggttcgcat
     8221 tacgaaaaac gtcacaatca gaattagaac gacgatgaac cagtaaagat ctaaatacca
     8281 aactccatct gccgagttaa ttggcgctct aaaccacaaa gctaagtgtg aaagtagaag
     8341 agcggcgaca aacggcgcac ccagatagat aatcatcgat cggatgcaag gttctttgtg
     8401 tatcgactct ttacctgatg gctcctggtg ttgcgaatcg taagcgagac atttgctgag
     8461 cccaacatcg aaaatgatga actctgccca aatgactcca ggaaaaaaga gagagatcgt
     8521 caataacaat ccgactgcgc caccaaccat ggagcctaca aacaggtaaa taagcgcagc
     8581 tttccattcg atgtcaggta aataacctat ttgggcgtaa tagagtgcga gtattccacc
     8641 gccgatggca aaaaagacta gccagatgac gagcggcgta ttatctccta gtattttacc
     8701 gatcaggttg ggagctagtt gtgtttgggt ttgcgcagtt ccgtttgagg agaccgacgg
     8761 tttgtcagca gtaatttctt gcattggatt accacctaaa gaggattcga ggagctagtt
     8821 aatgcagata cgaggctggc aaaacgatat tacaggtcct accgcacgtc aatctttata
     8881 tacggtgtgt gtaaaccttt atatacggtg aaagcctttg aggatatctc gcgtgcttaa
     8941 gcgaagaatc accgggcggc cgtgggggca ggtggtgggt gacgaggttt gtaggagtcg
     9001 atcgatcagc cagcgcatct tctcaggggc gaggggcatg ttgactttga tggcggcgtg
     9061 acaggcgagt gaggcggcga tttcgtcgcg catggtttcc ggggcgttgc tcttcttctc
     9121 ggcgtcgacg gtctccaaca actcgaagag catgttgcgt gcttcactgg caggcagatc
     9181 tgccggcacc gctctgatcg caacagtccg tcccgataat cgcatcaact caaatccata
     9241 cttctcgagc tcaggcatga gatcgtcgaa gactgctgcc tgcgcggggg tgagatcgaa
     9301 agtctcgggg acgagcatct gctgcgagtc ggtacgtcgc tccgcttcca gagcacgata
     9361 cttgtcgaaa agtacgcgct cgtgcgcgac gtgctgatcg atcaaaagca agccctgatc
     9421 gtcagtggcg atgatgaagc tctcgtcgag ttggcctagc gggcggatgt ttgtcgagag
     9481 cgattcaggg gtgacttcgc gggcaatctt ctccgctgaa ttcagtggag gaagggccgc
     9541 ggatttgacg gattgggacg cggatcggag catgatctcg aggtcgcgtg cgatctcgtc
     9601 gccggatggt tcgactggtg cacgtggtgg gggtgatgga ggtgggatga attcgattcg
     9661 aggttggggg gtgatcgagc ttacagcagc gatcgcggtc attatcggtg gttcgggtgg
     9721 cgggacgtag ctcgcgctcg ccagcgccgc ccgcaccgct tcgcgaacag catccgcaac
     9781 cgccgcttgc ctgcgaaaac gtacttccgt ttttgccgga tgcacgttca catcaacttc
     9841 ctcgagcgga gtctcgatga acaacaacgc tgcggggtaa acaccgtgcg gcaggatcga
     9901 tcgataagct tccgacaacg cgcgcccgat catgcgatcg cgcacgaaac gccgattcac
     9961 gaaaaggtat tgtgaatcgc gcgaggtgcg gcgatctcgc ggcgcggaaa cgaatccgct
    10021 gacgcgcgca aactcccgct cgccgccatc cacttcgagc agattcttca aaaactcttc
    10081 accaaagagc tggtacgcgc gctcgcgcag atccttcgcg ggcgccgtgc gcaccacttc
    10141 cctgccgttg tttataaacg tgaacgcgat ctcgggatgg gccaacgcgt aatgcgtcac
    10201 gagattcgtc aggtgatatg tctcagtcgc ttcggatcgc agaaacttgc gccgcgcggg
    10261 aatgttgaaa aagaggtcgc gaacgatgag cgttgtgccg cgcgggtgtg ccgcgtcttt
    10321 gacgtcgcgc atgcgcccac catcgatcgt gacgcgcgtg gcggcgttcg cgccttcgat
    10381 gcacgtggtc aactcaactt tcgcaaccga ggcaatcgac gcgagcgcct cgccgcgaaa
    10441 tccgagcgtg ccgatcgcag cgagatcgtc tgtttcgcgg atcttcgaag tggcgtggcg
    10501 ttcgaaagca agaatcgcgt cgtcgcgcac catgccttcg ccgtcgtcgg aaattttcaa
    10561 caggcgacgc ccgcccgctt cgatctcgat cgtgatgcgc gtggcgccgg cgtcgatcga
    10621 gttttcgacc agctctttgg cgacggaggc cggacgctca acaacttcac cggcggcgat
    10681 ttgattggcg acgtggtctg cgaggacgcg gattttcgac attctgcttg aaataggacc
    10741 aatagaactt atcggaccta taaatagaca atctggtgtt cgttagagcc aagattaatc
    10801 gcgttgaaga tccactcgat cacgtaagtg ccgtggcggg ccagcagcga cgtgatgttg
    10861 atgtgacgct cctgtagatc tttgttcgga tagagctgat cgaacgcgcg ctgcaattgc
    10921 cggtgcgccg cttcgtcacg tgtcatctgc gcgcgcacga aacgctgctt caaaccgtca
    10981 agctgatagt tgatcttctt gcgccccgtt tcgagcgcac tcgcgagcgt cggatcgatc
    11041 gattgaagct cctggcgcaa acgatcgagc tcgtggttca tgttctgctc cgcgtcggcg
    11101 aagagcttcg cactgtcagc cgcaagatgc tcctccacga cgcgcttgat caccggatcg
    11161 agtccttcga agaaatccgc cagcgtcagg tcgtatcttc tgagcaagcg accggtgtgg
    11221 tgctcgatca tcgtcagact cgaacgcggc aggatcggcg tcgccggtcg atcaagtacg
    11281 cgatagacct cggcggtctg cgcaaagtaa gcaatctcgg cagcgccacc gtagtaagcg
    11341 atcgtcggca aaaggtaatc ctggaccact gcgcgtaggg tcacgttcgg actaaaccgc
    11401 tcgggctttt cgtgcgcgag tgtggcgagc tcctcggcgg agtattcctg ctcgctggct
    11461 tttgttctgt actttccatt ctcgattcgc acgaccgcat gtcgccggtg ttcctcatcg
    11521 tgcagaaaca gtggaaacga attcgcagtc gcgagcacct gcgcgtgata acccgttcgc
    11581 accagctcag cactgcgttg ctcgagagcc gaggcgatct caggcgcgcg acgggcagct
    11641 tcggaataga gcggcgcggc gagttgtttc aacgccggat cgagtgggtc gaggaagatc
    11701 aggccatagc gaccgagcag cgcagtcatc atcgacgcga agctttcgac gtagccgcga
    11761 cccggctgcc acgcactctt cacgagcgcc ttcatgtcag ccgcgaattc cgatgcgggc
    11821 aacaactcaa acagctcatc cacgactgtg ctgatcgagt cgtcaagctt cacctgcccg
    11881 acgggttgtc cttcacgatg cagggcttca gaaacagcaa cacttttgag ctggcaatca
    11941 cgaccgataa gctccgcttt cgcgacttcg gcaaaatcgt gatcctcagc agcgatccaa
    12001 aacacgggca cggctttcgt gcctcgttgt tggaggcaac ccgcgagttt cacggccgag
    12061 agcgctttgt agaccgtgta gagtgggcca gtgaacaaac ccgcctgctg gcccgaaacc
    12121 accgcgatgc aatcctcatc gcgcagcaac gccagattct ttaacgtctc ctcgcctgcg
    12181 ccccaacgtt gattcatcgc ctcgagtgca tcacacacac gtgcgcgatc gacgcgatag
    12241 gcgctcaaga cgtccggcac gcgttgttgt aattcgtgat gaaagcgaac ggctgaagga
    12301 taaaagttgc gcagcgcgac cgggtctttc aggtaatcga gaaataggcg cgactggtgc
    12361 ggaattagat cgaacggaag cgactcgacg cgcagtcctg attcttcggg ggtgctataa
    12421 caagcggctt cgactgtact caaggtgttg atactcctgc gcgggttatt ctacaggtct
    12481 gagtgcgggg cgcaaagtta gtgtccgata aacgttagtt tgtcgcgaca ttccgacaag
    12541 ctaaagctta tcggacatca aactaatcct gtcgaggact gacttgatct ccgcgcgtgc
    12601 cgcgtcgtcc ggtgcgcgta atggcgcacg caccgcgcca ccacgatacc cagcaaattc
    12661 aagcgccgcc ttcaatccgc cgattccata cttcgtcgtt acggcggatg cgagcggcgt
    12721 caactcttcc tgcaagtgct ccgcatcttc cccggcggca aatgcccgaa agatctcgac
    12781 gcaaacctcg ggtacgacac atcccacggc gagaattcca cccgtcgcgc ccgcccgcaa
    12841 cgcatcgagc agcaccgttc cattacccgt catcaccgcg aagtcgctcg ggcaaagctc
    12901 gaccgtacga cgaaagccgg ccacatcgtt cgagctgtct ttcacaccga tgatgtttgg
    12961 atgttcactc agacgcgcga cagtttctgg ctcgatcttg attcccgtga gcggcggcat
    13021 gctgtaaagc agcaccggca ccggcgccgc ctcagcaacg gccgtgtagt aatttacgag
    13081 cgtctcctgc gtgatcgccg gacgataaaa ataaggcgtg atgacgagca ctgcctccgc
    13141 acccgctcgc gctgcgttct tgatctcttt gatggtattg acggtgcttt gctgtccggc
    13201 gccgacgatc aaaaccgagc cggtcgccgc acgcgagact tcgataactt cgagatactc
    13261 acgctcgtcg agatgaacgc gctcccccgt cgaaccgagc acaacgaagc caatcacgcc
    13321 acgtgcgctc cactctttaa tattagaggt gatgtccgcc gctgagatct ggccgtcggg
    13381 atcgaatggt gttgttgtcg ggagaaggat gccctttaat gcgcgcatgt tagacaggat
    13441 gacacgattt acagaattaa caagattaat cagtgaaaca gatctctaac tccgatcgcg
    13501 aagaatatat tcccgcgacc ttcacccggt ccctgattga cgttccacgc ataaccgagc
    13561 gtgaagtgcg ccgtcgtcgc cagctcgaag cgaatgcctg cacccggacc ataacgggtg
    13621 ccgccaaacg ctccgctacg cgggccgatc cgcgccatat caaacacgaa caccggactg
    13681 atcgaataga tgtttacatc gttgaagagc gtgttcaaag tgcggcgcgt gaaagccatg
    13741 tcgtccttcg ctttcttccc cgccgctttt gtatcgatct gattaaactc agcagttaat
    13801 tgtgtgcgct gtgtctcgac ccggctggtc gccgatgtga gcgatggctc cttcaacgtg
    13861 ccattcagat ctttctcaca tgcctgctgc acgttcagta actggtcgtc gtcaggatct
    13921 tcggagagca cctccgcaat ggagccgtaa ggaacacgcg acgtcgaaat cgcattcctg
    13981 attcgtctgg atacggcttt gatcgcatct gtgcaggcct cgaactgatc ctccaactgc
    14041 ccagcatgtg ttccctgcgc agtgttgacc accgtttgta aatcctggag agcggtttgc
    14101 gcggcgggca attgtgtgac gacgtttgcg tagtgcggat cctttgagta gtaaaaaacc
    14161 tcgagcgtgc tggtcacact cgtgatcgag ccctcgagct ggtcgttgaa ttcaggatca
    14221 ctggtgagct cttcaggaac gagcgtctgg cgccagattc cataagctgc cgtgaggttg
    14281 taactgacga aactccggcc gccggcgcct tcggcagttt ggaaaaaacg acttccggga
    14341 atcgcgcgaa ttaccggatt agcccgaatc tgccaatcgt cactcgcgat caggaactct
    14401 tcattgttgc cgccaaaaaa tctctcgggc agcggcacct taccggggat ctgaagttta
    14461 cccatcgtga agcgcgattc cagatcgagc aggtgatggt tgccgagtga ataccagaat
    14521 tgatgccgtg catcgaagat atgcttccgc cagtcgactc gtgcggctgg tccaactgaa
    14581 cccaactcga gaccatacga cgctgaaaag atgttgcgtg caaaccgtga atccagcccg
    14641 ccatagagtt tcaaagtgcc gaagcctgca ttcctgatgg tgttgggcgc gagtatcaaa
    14701 ttgccggctt cagtttggcg attaccacca tcgagggcag cgccaaagcg aagactcata
    14761 ttgccgccag ccaaaggcct ggtcacgccc gcgaaatgaa gcatcacgtg accgcctctg
    14821 atatcgccgg cgccagtcgg cagcgaatag tgtgaaaagt tgacgagcca gtctgcgtgg
    14881 gccaaccagg cgggatgctc gactggcgaa tcgtatgagc cttccaacgc ggcggaaact
    14941 tcgtgcattc tcgaagaagc ttcgcccttg atgagcgccg agttgaatgg cagcttcccg
    15001 atccggcgcg gcttgatctc gaagcgtcca ccgccgaaaa gcaggttagt ggagtcgtaa
    15061 ccaccggtag gcacgaaatg aaacgggctg gattcctgtg tcagggcggt agtttgtccg
    15121 gcaggagttt gcggcgtctc gcgttccttc acacgctctt ccgggcgagc gctcaacacg
    15181 ggcaggatct gcgttgagta aatcctgtaa atgacatcga cctgtccgtc cgtgcaattc
    15241 tggacgctca caaactcgag cctgaattgg acgcgagcat cgctcgtctc gggcaggaag
    15301 ttttgatcct caattatctt gagcgccttg tcagagacgt cactgtaagt aaacggcttg
    15361 ccttgtatca gctctgtgat ctgggcactg gcgcgcttct ggcgtgcctt tacccacggc
    15421 agaaacagga acggatcgtc gatgcgaata ctgcgaacct tgtaattcgc tgtttcgagg
    15481 ttgataccgt cgcatgaacc gacagactgg tctgtctcaa caatcttttt atcggactga
    15541 cctgcct
//
LOCUS       DASSFQ010000309         8023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1636654, whole genome shotgun sequence.
ACCESSION   DASSFQ010000309 DASSFQ010000000
VERSION     DASSFQ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636654"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..311)
                     /locus_tag="VFH96_11150"
     CDS             complement(72..311)
                     /locus_tag="VFH96_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET6974575.1"
                     /translation="MRKRKLLDRLKNNPKEATFRDVRTLLIQHGFQLERVTGSHHIFS
                     RPDATFAIPVHAKRVKSAYVRQVIKLLEQSKGKPE"
     gene            450..2402
                     /locus_tag="VFH96_11155"
     CDS             450..2402
                     /locus_tag="VFH96_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET6974576.1"
                     /translation="MLEVFGPDGLIAAAHPEYEYRPGQVEMARAVMRAFEEKRHLIVE
                     AGTGTGKTLAYLVPAVAAALGGNGRVIVSTGTKNLQEQLMEKDIPFLQNVLPKPFAAT
                     YMKGRNNYLCLNRLARAQSAPVLEGLDEVDYFEEVSQWSRETEIGDRAELSNLPESLS
                     FWRHIDARSESCLGQKCSDYDACFITRMRSRAQDADIVVVNHHLFFADLSLRNGNYGS
                     VLPDYTAVILDEAHLVEEVASEYFGAQVSNYQIDDLVRDLGMLTIEEAEVDQELTHSV
                     ARMSRFADNFFMSFRDGRGEDGRYPIIPGTFARRKSSGDMEATPLGELYMALEGAIAR
                     AETTLDALKEKAPDVENLIRRLREIRFELEFIVTGTDKKFVYWLERRNRGVFLRASPI
                     DVAGLLQDKLFEEVPTVVLTSATLSSGGNFAFIRDRLGLDTADDLIASSSFDYESQAI
                     LYLPSKMPDPRDRNWGLAAADEVIRILNATEGRAFVLSTSLAGMQSLFENVWAEIDYP
                     CLVQGSASKGQLLKKFRETPNAVLFATSSFWQGVDVRGEQLSCVIIDKLPFAVPTDPI
                     VAARQRSIEDSGGSSFYEYSVPQAIITLKQGLGRLIRSTTDRGVLAILDPRLRTKGYG
                     RTFLQSLPPCRVTSRIDDLASILSSD"
     gene            2405..2785
                     /gene="queD"
                     /locus_tag="VFH96_11160"
     CDS             2405..2785
                     /gene="queD"
                     /locus_tag="VFH96_11160"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HET6974577.1"
                     /translation="MPGQFEVMIERNFSSAHQLRGYRGKCENLHGHNYKIEIYARGRE
                     LDNVGLLVDFVELKAAADEVVTYLDHRNINELPPFDELQPSAENLAKYVLERVAQRVG
                     DERVQIYKVRCFETPTSVATYQID"
     gene            complement(2804..4213)
                     /locus_tag="VFH96_11165"
     CDS             complement(2804..4213)
                     /locus_tag="VFH96_11165"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET6974578.1"
                     /translation="MGFLRLFVFILIFVTSMAAQQPPGSPTPTPTPMPPPTVKIDRYR
                     AAAARIIGAALTSDRAYSRLAHLTDHIGNRLSGSQNLERGIEWAISEMKRDGLDNVCG
                     EKVMVPHWVRGEESLEMLAPVPRKLQMLGLGNSVGTPAEGITAEVVVVRNFEELDRLG
                     EQVRGKIVVYNAPYVNYGATVEYRVRGASRAARYGAVAALVRSITPVSLQTPHTGAMN
                     YDPNQPKIPTAAVTIEVAEFLQRMNDRGDHPRLRLKMEAKFLPDAESANVIAELKGSE
                     KPDEIVLISGHFDSWDVGQGAHDDGGGCIVAWEAARLLKELGLKPRRTIRVVLWTNEE
                     NGLRGGNAYRDAYRADVAKHILAIESDSGVYRPEGFGLAATAPLQVRSNMIEIARLLS
                     GIGADQIAADGGGADIGPMMREGVIGASLDVDGAHYFDIHHTQADTLDKVNPRELALC
                     VATMAVMAYVVADLPESFR"
     gene            complement(4282..5460)
                     /locus_tag="VFH96_11170"
     CDS             complement(4282..5460)
                     /locus_tag="VFH96_11170"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6974579.1"
                     /translation="MHLPHVARLRGIDALRGAAALGVVLYHAVEQGKHVLPGNLFHYP
                     IRVVQFASSFGYIGVFLFFVISGFCIHLQWAKSRAAGVEPEIRFGSFWKRRIRRLYPP
                     YIITLLLFMLLTAATVGLNVTRFFVYDLGMHLLMLHNLDPHTCYTINGVFWTLAIEEQ
                     LYLAYFLLLFIRVRWGWSVTLAVCLLARLAWMGLSHVVWLKTGFGIPVPEAAASHWFT
                     WALGALGVEVYFGLTTLRGWSRDLRLATVLIVIASALSTYLPWISKDTLLHDTSWFLI
                     HPLWGLGFFIVVNRTVLAEQSWLRQARLPSLVSVFSTLGVFSYSIYLTHELVIMQSWL
                     WVNPGWWQLANVFVVVLPSTILFAWVFFWFCEKPFMVKSAPSDKAVPALTPQPSAAGD
                     "
     gene            complement(5572..6960)
                     /locus_tag="VFH96_11175"
     CDS             complement(5572..6960)
                     /locus_tag="VFH96_11175"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6974580.1"
                     /translation="MENKEVRILVVDDEPTMSDSLRQNFVEEGYSVDTAATGAAAIEL
                     FDQGGHHLAICDLQLPDVDGMEVLRHMKDAKPTTEVIVVTGYGSVQKAVEATKAGAFY
                     FVEKPFDFEQLQPLVEKALERRELVAETANMRRQLSTRAEYFNIIGASKPMQQIYETI
                     ESVAKSDANVLIVGESGTGKELIANAIHYQSLRSKKPFIKVNCAALPKELIESELFGH
                     TKGAFTGAHADKEGLVQHAAGGSLMLDEIAEMPIELQPKLLRVLQERSYRKIGSEKTY
                     AVDFRLVCSTNRPPADAIRDGLLRDDLFYRISTITIHVPPLRERNEDIQLLTEHFLHM
                     YAQKYERPITGVSQAAYQRLFAHSWPGNVRELQNVIERAVLLAKDKQIEPIDLPFDNG
                     TLPEGTSSNVTWDVPPNMTLEDIERLVIERTLQRTGGNKQAAANLLGIYRPRLYSKIR
                     KYNIDVPSLVSA"
     gene            complement(7030..7692)
                     /locus_tag="VFH96_11180"
     CDS             complement(7030..7692)
                     /locus_tag="VFH96_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974581.1"
                     /translation="MKTLMSRPDETERLQDLGRASMQIVHDLKNQLNGLKLYATFLRR
                     RIEKNERPGDEQETIAKLINGLDRAADDLSTIVKYGQPVELKKQPGVEVDKILRGVCA
                     SATEAGSRIVLDSSSASVQGDLDLIKLTDAFKWLCAAAMKHKQNQDEAGEIFVTTKSS
                     DGAQAVIEWSGLRELDHDPFRTFAGSDEVKLALAAKIIEAHGGSAKFIDRKFVVQLPL
                     NE"
BASE COUNT         1783 a         2212 c         2228 g         1800 t
ORIGIN      
        1 tgccggaact tccgccacgt agcctccctc ttcggctgga tagataatta ttgggtatag
       61 gaagtcctct gtcattccgg ctttcctttc gattgctcga gcaacttgat cacttgcctg
      121 acgtaagccg attttacacg ctttgcgtga acaggaatag caaacgtggc atctggcctg
      181 ctgaaaatgt gatgactccc ggtcacccgt tcaagctgga aaccgtgctg aattagcaac
      241 gtgcgaacat cgcggaacgt tgcttctttt ggattattct tgaggcgatc aagtagttta
      301 cgttttcgca tagctaagtg aggtagcttg gccccgcatt ctaagttaaa gatagacaaa
      361 aataaagcac gtacgcctca atctactgaa tttgccagtc gtgttatcct ctgtctccca
      421 cagtttccga acagggtttt gcaaacaaaa tgcttgaagt ttttggtccg gatggactga
      481 tcgcggcggc gcatccagag tatgagtatc gacctggcca ggtggagatg gcgcgggcag
      541 ttatgcgcgc gtttgaggag aaaagacatc tcattgtcga ggcgggtacg ggcacgggga
      601 agacgctcgc gtatctcgtt ccggcggtgg ctgccgcgct tggtgggaat gggcgcgtca
      661 tcgtctcgac gggcactaag aacctgcagg aacagttgat ggagaaagat attccatttc
      721 ttcagaacgt cctgccgaag ccgttcgccg cgacctacat gaagggtcgc aacaattatc
      781 tttgcctcaa ccgtctcgcg cgtgcgcagt cggcgcctgt gctcgaagga ctcgacgagg
      841 tcgattactt cgaagaggtt tcgcaatggt cacgcgagac tgaaatcggc gaccgcgctg
      901 agctgtcgaa cctgcctgag tcgctctcgt tctggcgtca catcgacgcg cgctccgaat
      961 cgtgtctcgg tcagaagtgt tcggattacg acgcgtgctt catcacgcgc atgcgcagtc
     1021 gcgcgcagga tgcggacatc gtcgtcgtca atcatcatct cttcttcgcc gacttgagct
     1081 tgcgcaacgg caactacggc tcagtcttgc ccgattacac tgcggttatt ctcgacgaag
     1141 cacatctagt cgaagaggtg gcgtctgaat acttcggtgc gcaggtgtcg aactatcaga
     1201 tagacgatct cgtccgcgat ctcggcatgc tcacgatcga agaggcggaa gtcgatcagg
     1261 agctgacgca cagcgtcgcg cgcatgtcgc gttttgccga caacttcttc atgagttttc
     1321 gtgatggacg tggtgaggat ggtcgctacc cgatcattcc gggcacgttt gcgcgccgga
     1381 agtcgagtgg agacatggaa gcgacgccgc tcggcgagtt gtacatggct ctcgaaggcg
     1441 cgattgcgcg cgccgaaacc actctcgatg cgctcaaaga aaaagctcca gatgtggaga
     1501 acttgattcg tcgtttgcgt gagatccgtt tcgagctcga gtttatcgtc accggaacag
     1561 acaagaaatt tgtttactgg ctcgaacgac gaaatcgcgg tgtgttcctg cgcgcttcgc
     1621 cgatcgacgt cgccggacta ttgcaggaca agttgtttga ggaagtgccg acggtcgtgc
     1681 tgacatcagc gacgctctcg agtggcggca acttcgcctt cattcgcgat cggcttggcc
     1741 tcgacacggc ggacgatctg atcgcgtcat caagtttcga ctacgagagc caggcgatac
     1801 tttacctgcc ttcgaagatg cccgatccga gagatcgcaa ctggggcctg gctgcggctg
     1861 acgaagtcat acgcatactt aacgcgaccg agggacgtgc atttgtgttg tcgacgagtc
     1921 tggccggaat gcagtcgctg ttcgagaacg tctgggccga gattgattat ccttgcctgg
     1981 tccagggcag cgcttcgaag ggtcagctac tgaaaaagtt tcgcgaaaca ccgaatgcgg
     2041 tgctgtttgc gacctcaagt ttctggcagg gcgttgacgt acgtggagag cagttgtcgt
     2101 gtgtgatcat cgataagctg ccgtttgcgg ttccgaccga tcccatcgtc gcggcgcggc
     2161 aaagatctat cgaagacagc ggcgggtcga gtttttacga atacagcgtg ccgcaggcga
     2221 tcatcacgct caagcaggga ctcggacggt tgatacgcag cactactgac cgcggcgttt
     2281 tagccatact cgatccgcgc ctccgaacga aaggttatgg tcgaacgttt ctccagagcc
     2341 tgccgccctg ccgcgtgact tcgcgaatcg acgaccttgc gagtatctta tcgtcagact
     2401 aattatgcct ggacaattcg aagtaatgat tgagcgaaac ttctccagcg cgcatcaact
     2461 gcgcggctat cgcgggaagt gtgagaacct gcacggacac aactacaaga tcgagatcta
     2521 tgcacgcgga cgcgagctgg ataatgtcgg tttgttggtt gactttgtcg agctgaaagc
     2581 ggcggcggat gaggtcgtga cgtacctcga tcaccgaaac atcaacgagt taccgccgtt
     2641 tgatgagttg cagccgtcag cggagaacct ggcgaagtac gtgctggagc gcgtggcgca
     2701 acgtgttggc gacgagcgcg tgcagatcta caaggtgcgg tgctttgaga caccgacgag
     2761 cgtggctact taccagatcg attgaaacgc ggattaacgc ggatcatctg aacgattcag
     2821 gcagatcagc aaccacataa gccatcaccg ccatcgtcgc gacgcacagt gcgagctcgc
     2881 gtgggttgac cttgtcgagc gtgtcggcct gcgtgtggtg aatgtcgaag tagtgtgctc
     2941 cgtccacgtc aagactggca ccgatgacgc cttcgcgcat cattggtcca atgtccgctc
     3001 caccgccatc tgctgcgatc tgatccgcgc cgatacctga taacaacctc gcgatctcga
     3061 tcatattgga acgaacctgc aacggcgctg ttgctgcaag gccgaagcct tccggtcgat
     3121 agactcccga atctgactcg attgccagaa tgtgttttgc aacgtctgcg cgataagcgt
     3181 cgcgataagc attgcctccg cggagcccgt tttcttcgtt agtccagagc acaacgcgga
     3241 tggtcctcct gggcttcaac ccgagctctt tgagtaatcg cgccgcttcc catgcgacga
     3301 tgcagccgcc gccgtcgtca tgcgcgccct ggcccacgtc ccacgaatcg aagtgcccgc
     3361 tgatcaagac tatctcgtcg ggcttctccg atcccttaag ctcagcgata acgttcgctg
     3421 attctgcgtc gggtagaaac ttcgcctcca tcttgagtcg caagcgcgga tggtcaccgc
     3481 gatcgttcat gcgctgcaaa aactccgcca cttcgatcgt cacagccgca gtcggaatct
     3541 tcggttgatt cggatcgtaa ttcatcgctc ccgtgtgtgg cgtttgcaga ctcacaggcg
     3601 tgatcgagcg cacgagtgcc gcgaccgcac cgtagcgagc ggctcgtgac gcacctcgca
     3661 ctcgatactc gacagtggcg ccgtagttca cgtagggcgc gttatagacg acgattttcc
     3721 cacgcacctg ctcgccaaga cgatccagct cttcgaagtt gcggacgacg acgacttcag
     3781 cggtaattcc ttcagccgga gtgccgacac tgttgcccaa accaagcatc tgtagctttc
     3841 tcggaacggg cgcgagcatc tcgagactct cttcaccgcg cacccagtgc ggcaccatca
     3901 ctttttctcc gcacacgtta tcgaggccgt cgcgtttcat ttcgctgatg gcccactcga
     3961 tgccgcgctc caggttttgc gagccgctca gacgattacc gatatggtcc gtcaggtgcg
     4021 cgagtcgcga ataagcgcga tcgctggtga gtgcggcgcc gatgatgcgt gcggcggcag
     4081 cgcgataccg atcgatcttg acggttggtg gcggcatggg ggttggtgtt ggagtgggag
     4141 agccgggtgg ttgttgtgct gccattgagg tgacaaagat gagtatgaaa acaaacaggc
     4201 gaagaaaacc cacgtttacg attcctccta acggaccggt tcttagcgta agcttagaca
     4261 ggatttacaa gattagcagg attagtcgcc tgcggcactc ggttgcggtg tcaaagccgg
     4321 cactgcctta tcactcggcg cgctcttcac catgaacggc ttctcacaaa accagaagaa
     4381 cacccacgca aacaagatcg tcgacggcaa aacaaccacg aacacattcg ccagttgcca
     4441 ccaacccgga ttcacccaga gccacgactg catgatcacg agctcatgcg tcaaatagat
     4501 tgagtacgaa aagacaccga gcgtcgagaa gacggaaacg agcgaaggca acctcgcctg
     4561 tcgcagccag ctttgttcgg cgagcacggt gcggttaacg acaatgaaga acccgaggcc
     4621 ccacaacgga tggatcaaaa accagctcgt atcgtgcagc aaagtgtctt tcgaaatcca
     4681 cggcaggtag gttgacagtg cagaagcgat gacgatcagc acagtcgcga gccgcagatc
     4741 gcgtgaccag ccgcgcagag tcgtcagtcc aaaatatact tccacgccca gcgccccgag
     4801 cgcccacgtg aaccagtgtg acgctgccgc ttccggcacg ggaatgccaa atccggtctt
     4861 cagccacacg acatgactga gtcccatcca cgccaatcgc gccaacagac aaacagcgag
     4921 cgtcacgctc cagccccagc gcacgcgaat gaagagcagc agaaaatacg cgagatagag
     4981 ttgttcttcg atcgcgagtg tccagaacac gccgttgatc gtgtagcacg tgtgtggatc
     5041 gagattgtgc aacatcagca ggtgcatgcc gaggtcgtag acgaagaagc gcgttacgtt
     5101 aagaccgacg gttgccgcgg tcagcagcat gaacagcagt aacgtgatga tgtatggcgg
     5161 ataaagccgg cgaattcttc gtttccagaa cgagccgaag cgaatttcag gctcgactcc
     5221 tgcggccctg gacttggccc attgcaggtg aatacaaaaa ccggagatca caaagaacag
     5281 aaagacgcca atgtaaccga acgaactcgc gaattggacc acacgaatag ggtagtggaa
     5341 gaggttgcct ggaagaacgt gtttgccttg ttcgacggcg tgataaagaa cgacgcccaa
     5401 cgccgcagcg cctctaagcg cgtcgatgcc acgaaggcgc gccacatggg ggagatgcat
     5461 aggaaaaatt aattagccac gaaaaggcac aaaagtcaca aaacatctta ctgaaaacga
     5521 gctcgactcg tctgccctat ttgtgcttct tgtgcctttt gtggccagta ctcatgccga
     5581 taccaatgaa ggcacgtcga tattgtactt ccttattttg ctgtagagtc gcggccggta
     5641 gatgcccaaa agattggccg ctgcctgctt gttccccccg gttcgttgca gagttctttc
     5701 aatcacgagg cgctcgatgt cttcaagcgt catgttcggt ggcacatccc acgtaacatt
     5761 cgacgacgtt ccttccggaa gcgtgccgtt atcaaaaggt agatcgatcg gctcgatctg
     5821 tttgtctttc gcgagcagca ccgcgcgctc gatcacgttc tgcagctcgc gcacattccc
     5881 gggccaactg tgagcgaaaa gtctttgata cgcagcctgc gaaacacctg tgatcggccg
     5941 ttcatatttt tgcgcgtaca tgtgcaggaa gtgttcagtc aagagctgga tgtcttcatt
     6001 gcgctcgcgc aaaggcggca cgtgaatcgt gatcgtcgag atacgataaa acagatcgtc
     6061 gcgtagcaag ccgtcgcgaa tcgcatcagc cggtggacgg ttggtcgagc agacgagccg
     6121 gaagtcgaca gcgtaagtct tctccgatcc gatcttgcga taacttcgct cctgcaacac
     6181 acgcaagagt tttggctgca actcgatcgg catctccgca atttcatcga gcattaacga
     6241 gccgcccgca gcgtgttgga ccaacccttc tttgtctgca tgcgcgccgg tgaacgcgcc
     6301 tttcgtgtga ccaaacaact cggattcgat caactctttc ggcaacgccg cgcagttcac
     6361 tttgatgaag ggtttcttcg aacgcagcga ctggtagtga atcgcgttcg caatcaactc
     6421 tttacccgta cccgactcgc ccacgatcag cacgttcgcg tctgacttcg ccaccgattc
     6481 gatcgtctcg tagatctgtt gcatcggttt cgacgcaccg atgatgttga aatactcggc
     6541 gcgggtggag agctggcgcc tcatgttagc tgtttccgca accagctcgc ggcgttcaag
     6601 cgccttctcg acgagcggct gtagctgttc gaaatcaaat ggcttctcga cgaaatagaa
     6661 cgcgcccgcc ttcgttgctt cgacggcttt ctgaaccgag ccgtaacccg taaccacgat
     6721 cacttcggtg gttggttttg cgtctttcat gtgacgcagc acttccatac catccacgtc
     6781 gggcaattgc aggtcacaga ttgcaaggtg atgcccgccc tgatcaaaaa gctcgattgc
     6841 ggccgctccc gtggcggcgg tgtcgacaga gtaaccttcc tcgacgaaat tctgccgcag
     6901 agagtcggac attgttggtt cgtcgtctac taccagaatc cgcacttctt tgttctccac
     6961 ctttttctcc tctctcgaaa tcgctgcgcc ttgggacatg atgattactc ccgtgtataa
     7021 agttaagaat tactcattga gcggaagctg aacaacgaac ttcctgtcta taaattttgc
     7081 cgagccgccg tgggcctcaa tgatctttgc cgcgagcgcc agtttgacct cgtcgctgcc
     7141 cgcgaaagta cgaaacggat cgtgatccaa ctcgcgcagg cccgaccact cgatcacggc
     7201 ctgtgcgcca tcagacgatt ttgtcgttac gaaaatctca ccggcttcgt cctggttttg
     7261 cttgtgcttc atcgcggcgg cacatagcca cttgaaagcg tctgttagct tgatcaggtc
     7321 gagatctccc tgaaccgagg cagaactgga atccaggact atccgcgagc cggcctcagt
     7381 agcgctggca cagactccgc gcagaatttt gtcgacctcg acgcccggct gctttttcag
     7441 ttcaacgggc tgtccatact tcacaatcgt cgataaatcg tcggcggcgc ggtcgagtcc
     7501 attaatcaac ttggcaatcg tctcctgctc gtcgccgggc cgctcgttct tctcgatccg
     7561 gcgtcggagg aacgtggcat agagtttgag accgttgagt tgattcttga ggtcatggac
     7621 tatttgcatt gatgctcgcc cgaggtcctg gagtcgttca gtctcgtctg gcctcgacat
     7681 taaagtcttc atctgggcaa atctttcctc agttctaaaa tagggggccg cagggggagg
     7741 ggaacctcac agccaggccg agtgtatgaa ttaaactctg gctcaagatc cgtttgtgtt
     7801 gcgtttcaat gcgggatggt agcaaaaatc aagcgcgtat tctagtgata cagtaaaatc
     7861 aataacttgc ggggaacaga gggcggtaag attgaaagca gaagcgggta cgaagatgaa
     7921 agcggaggct catccaacta cggtcttgct cgtagaagat accgaagaca accggttcat
     7981 gatgcggcgt ttgttggaaa tgacgggcta ccgggtagtt gag
//
LOCUS       DASSFQ010000310         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_583703, whole genome shotgun sequence.
ACCESSION   DASSFQ010000310 DASSFQ010000000
VERSION     DASSFQ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583703"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFH96_11185"
     CDS             <1..681
                     /locus_tag="VFH96_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6974582.1"
                     /translation="IGQRLDVGMFESPTPAEIIGIVGNVRYDSLVDEALPAVYFPHPD
                     LAYPFMTLVVRTDGDPAAIAPAIQREIRGLDPNQPVSDVRTMNQVMSEWVARSRFNTL
                     LLGLFAGLATLLSAVGIFGVMNYSVALRTRELGLRLAVGAQPRQVLLLVLRQGLGLTV
                     VGVVLGLAAAFALTRLLSGLLFGVAAVDVTTFATISLLLVIVSLLACYLPARRAMRID
                     PLSALRYE"
     gene            728..2386
                     /locus_tag="VFH96_11190"
     CDS             728..2386
                     /locus_tag="VFH96_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974583.1"
                     /translation="MATLERTVGSKLGWMIAGRLVTALLLSVVGTLWTGQASQSINKS
                     LGLLTIVACLTIFYALIFRLSKNILMQARFQLAVDVLLVTWLVWNSNVIQSPYVALYI
                     VIIAVSSLFLGPREAVVTSVGCAVAFTACALEITGFMSETDPSRLVGGSVSQTIQWVG
                     LFDVAFFVVGLLSARLAERQSRSDVRLAAATQSLANLRALHERIVASIRSGVVTTDLE
                     GRIFTFNVAAQEITHYSEETIRGQDASILFSDLKEHVAQTLTALKKGDNSPRFETSCL
                     TSEGMRLRLGYSIAPLSTEAGETTGMVITFQDLTQVRSLEETSRRQDRLAAIGRMAAS
                     IAHEIRNPLAAMRGSIQMLRSEMNSDPAQTELMEIILRESDRLNRIITDFLSYARPRS
                     LVQARVDVGDLLHQTFALMRHSPEIGANQSIVEELPDDALYAEADEGQLKQVFWNLAR
                     NALQAMPQGGTLRATLEKNSHERIRITFTDSGRGMSPEQVEHLFEPFSSTTGGTGLGL
                     SIVYQIIRDHGGTINVRSLVGQGTTITVELPVTTEPSAVAPGQS"
     gene            2443..3957
                     /locus_tag="VFH96_11195"
     CDS             2443..3957
                     /locus_tag="VFH96_11195"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6974584.1"
                     /translation="MANLLIVDDELGMRQFLTHLFQREGHFVRVAENGHAALEQIRQL
                     APDLIICDIRMPDMSGVDLLREVRGLLPEVEVVMMTAFANVDTAREAFLLGAYDFVQK
                     PFDNDLLKETVARALKKISLIKEKEALLEENKALIQGQRTRGRLGNIIGRSERMRALY
                     QMIEQVAEVQSTVLVTGESGTGKELVARAIHDLSPRAEKPFVSVNCGAFTETLLESEL
                     FGYVKGSFTGANANRKGLFEAANSGTIFLDEIGEMSPAMQVKLLRVLQERKVRPVGAT
                     EETLVDTRVIAATNRDLASMVSANTFREDLYYRISVIPIELPPLRERAEDISELADHF
                     VQKFCAPTGRTLAVSENAMRLLERYSWPGNVRELEHTIERAVALERTNSIEPERLPEK
                     VTNYNPYRVAEAMEFPEEGINLTAHLDQLEKTYVLEALRRTAGNQTNAAELLRLSVRS
                     LRHLLDKHGARGLTAQMRDERRGPDSVPRRRATDQGPRRRSEDSGEFAAGAGEN"
BASE COUNT          873 a         1182 c         1189 g          884 t
ORIGIN      
        1 attggacaac ggctcgacgt cgggatgttt gagagtccga cgccggccga gatcatcggc
       61 atcgtcggta atgtgcgcta cgacagtctc gttgatgaag cgctgccggc tgtttacttt
      121 ccgcatcccg atctcgctta tccattcatg acgctcgtcg tgcgcactga cggcgatccg
      181 gctgcgatcg cgcccgccat tcaacgcgag attcgcggac tcgatcccaa tcaacctgtt
      241 tctgatgtac gcacgatgaa tcaggtgatg tcagaatggg tggcgcgttc gcgtttcaac
      301 acactgctgt taggactctt cgccggactc gccacactgc tctcagccgt cggcatcttc
      361 ggcgtgatga actattcagt tgctttgcga acgcgcgagc tcggcctacg actcgccgtt
      421 ggcgcgcaac cgcgacaggt gttgctactc gtcttgagac aggggttagg tttgactgtg
      481 gtaggcgtcg tgctcggact tgccgcagcg tttgcgctca caagactttt atccggcttg
      541 ctgtttggcg tcgcggcagt tgatgtgacg acgtttgcga cgatttcgtt gttgctcgtg
      601 atcgtgtcgc tgctcgcttg ttatttgccg gcgcgacgtg cgatgcgcat cgatccattg
      661 agcgcgctca gatacgaata gataatccgt cttcatctgt gtagatctgt gttacgcttt
      721 ggccccgatg gccacgctgg aacggaccgt aggctcgaag ctcggctgga tgatcgccgg
      781 gcgactcgtc accgcacttc tcctcagtgt cgtcggaact ctttggaccg gacaagcctc
      841 acaatcgatc aataagagtc tcggactgtt gacgatcgtc gcgtgtctca cgattttcta
      901 cgcgctgatc tttcgtctct caaaaaacat cctcatgcag gcacggttcc aactcgccgt
      961 cgacgtgctg ctcgtcacgt ggctcgtttg gaattccaac gtcatccagt cgccttacgt
     1021 cgcgctctac atcgtcatca ttgcggtttc gagtcttttt ctgggaccac gtgaagcagt
     1081 cgtgacgtct gtcggatgcg ccgtagcgtt cacagcgtgt gcgctggaaa tcacgggctt
     1141 catgagcgag accgatccct caagactcgt cggcggctca gtgtcgcaga cgattcagtg
     1201 ggtcggacta tttgacgtgg cgtttttcgt cgtcggtctg ttgtcggcgc gactggcaga
     1261 aagacagtcg cgctctgacg tgcgtctcgc tgcggcaaca cagtcgctgg ccaacctgcg
     1321 cgccttgcac gaacgcatcg tggcgtcgat ccgctccggc gttgtcacca ccgatctgga
     1381 aggacgcatc ttcaccttca acgtcgccgc acaggagatc acgcactact ccgaagagac
     1441 gattcgcggg caggatgctt cgatcctttt cagcgatctg aaggagcatg tcgctcagac
     1501 tttgactgct ttgaaaaaag gcgacaacag cccgcgcttc gaaacgagct gcctgacttc
     1561 agaaggcatg cgtctgcgcc tcggttacag catcgcgccg ttatcgacag aagccggcga
     1621 gacgacggga atggtgatca cgtttcagga tctgacccag gtccgcagtc tcgaagaaac
     1681 gtcgcgacgc caggatcgtc ttgcggcaat tggtcgcatg gcggcgtcga tcgcgcacga
     1741 gattcgcaat ccattggctg caatgcgcgg ctcgattcag atgctgcgtt cggagatgaa
     1801 cagcgatccg gcacagacag agctgatgga gatcattctg cgcgagtctg atcggttgaa
     1861 ccgcatcatc accgattttc ttagttacgc gcggccacgc tcgctcgttc aggcgcgtgt
     1921 tgacgtaggc gacttgctac accagacgtt tgccctcatg cgacacagtc cggaaatcgg
     1981 tgcgaaccag agcatcgttg aggaactacc ggacgacgcg ctctacgccg aagctgacga
     2041 aggccagtta aagcaggtgt tctggaacct cgcgcgcaat gcactacagg cgatgccgca
     2101 gggcggaacg ttgcgcgcga cgctggaaaa gaattcccac gagcggatcc gcatcacctt
     2161 cacggacagc gggcgtggca tgtcgcccga acaagtcgaa cacctattcg agcctttctc
     2221 atcgacgacg ggcggcacgg gtctcggtct ttcaattgtt tatcaaatca ttcgcgatca
     2281 tggtggtaca atcaatgtcc gcagtctcgt gggacaggga accacgataa ctgtcgagtt
     2341 accagtaacc acagaaccga gcgcagtagc gccgggtcaa agttaaccga tcacgacctt
     2401 aatagaagtt acccggcgct accgcgctcg gttccgtaag ccatggccaa tcttttgatc
     2461 gttgacgacg aactcggaat gcgtcagttt ttgacgcatt tattccagcg cgaaggacac
     2521 tttgtgcgcg tcgcggaaaa cgggcacgcc gcgctcgaac aaatacgaca gctcgcgccc
     2581 gatctgatca tctgcgatat cagaatgcct gacatgagcg gcgtcgatct gctgcgtgag
     2641 gtgcgggggc tgctaccgga agtggaagtg gtcatgatga cggctttcgc gaacgtcgac
     2701 accgcgcgtg aagcgtttct actcggcgct tacgactttg ttcagaagcc tttcgacaac
     2761 gacctgctca aagagacggt cgctcgcgcg ctcaaaaaga tctcactcat caaagaaaaa
     2821 gaagcgctgc tcgaagagaa taaggcgctc attcagggac aacgcacacg cggcaggctt
     2881 ggcaacatca tcgggcgcag cgaacgcatg cgcgcgctct accagatgat cgaacaggtt
     2941 gcggaggtgc agtcgacggt gcttgtgacg ggggaatccg gtaccgggaa agagctcgtc
     3001 gcgcgcgcga tccacgatct gagtccacgt gccgagaaac ctttcgtgtc agtaaactgc
     3061 ggcgcgttta ctgaaacgct actcgagtca gagttgtttg gttatgtgaa aggctcgttt
     3121 accggcgcga acgcaaatcg caaaggactc ttcgaagcgg caaacagcgg cacaatcttc
     3181 ctcgatgaaa tcggcgagat gtcgcccgcg atgcaggtga aactcctgcg cgtgttgcag
     3241 gagcgcaaag tcagaccagt aggcgcgact gaagaaaccc tcgtcgatac gcgcgtgatc
     3301 gcggccacga atcgcgatct cgccagcatg gtgtcggcca acacatttcg ggaagatctt
     3361 tactacagaa tttccgtgat accgatcgag ctgccaccgt tgcgcgagcg cgcagaagac
     3421 atttcagagc tcgccgatca tttcgtgcag aagttctgcg cgccaactgg gcgaaccctc
     3481 gccgtcagtg agaacgcgat gcggctgctc gagcgttact cgtggccggg aaatgttcgc
     3541 gagctcgaac acacgattga gagagccgtc gcgctcgagc gtaccaactc gatcgagccg
     3601 gaacgtctgc ccgagaaggt cacgaactac aatccgtatc gcgttgcgga agcgatggaa
     3661 tttccggagg aaggaatcaa cctcaccgca cacctggacc agcttgaaaa gacttacgtg
     3721 ctcgaagcgt tgcgtcgcac ggcgggaaat caaaccaacg ccgccgaact cttgcgtctg
     3781 tcggtgcgct cgctgcgaca cctgctcgac aagcatggtg ctcgtggtct aactgcgcaa
     3841 atgcgcgacg agcgacgtgg tccagattca gttccgcgcc gccgggccac ggaccagggc
     3901 ccacgccgcc gctccgaaga ctcaggcgag ttcgctgccg gcgccggcga aaattagcca
     3961 caaaaaggcg caaaaagcac aaaagaaaag gttcaaggtt cagcctttag ggtgcctgct
     4021 cggactgcag gcgagcacgc taaagcttga actctgaaca cttctccttt gtgctttttg
     4081 tgcctttttg tggctaattt tcgccggcgc cggcagcgaa ctcgcctg
//
LOCUS       DASSFQ010000311         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_145194, whole genome shotgun sequence.
ACCESSION   DASSFQ010000311 DASSFQ010000000
VERSION     DASSFQ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145194"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VFH96_11200"
     CDS             complement(<1..1551)
                     /locus_tag="VFH96_11200"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HET6974585.1"
                     /translation="MANNPNNERDSKVFALYEQLVEIEQRLIPTGLHVFGRASSAGEK
                     TDLLKMIASFDRPEAGLRALPEDLIDEAVRAFVADGTEAATAFLQTKGLDSEATRPVF
                     ALLETVTAKLETNNEIDSLVRALRGEFIEPGPGADIIQNPDILPTGRNTHALNPYGVP
                     SALAFARSEAVANSLLSRYQQENGRYPEAMALVLWGLDNIKTQGEGVAQALWLLGTRP
                     VRDALNRTTSVEVIPLSELGRPRIDVVMTVSGIFRDLFSPTVQLLDKAVRVVAQLDEP
                     LELNYVRKHVLQQVEEEACDLDDAAVRVFSNAPGNYGTNVNFMVMDSQWEREETLGDL
                     FVTRKCFAYGRDSKGRAIEGREARVAMDRALSRVEATYQNIDTFEIGLTDVDHYFEYL
                     GGVSKAVEKRAKSRPAIYLSDTLSREVKIRSLEETVRLETRAKTLNPKWYEGMLKHGF
                     RGVAEIENHVSNTFGWSATADAVDDWIYSEVANTFLFDENMLERLRDLNPHSAHSLTQ
                     RLLEANSRG"
     gene            complement(1557..>2815)
                     /locus_tag="VFH96_11205"
     CDS             complement(1557..>2815)
                     /locus_tag="VFH96_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6974586.1"
                     /translation="RANLAKINMDRKVLEKIAQLCADLKVDGHRGELTIMRSARALAA
                     FEGRKSVTEEHVKRVSAMALRHRLRRDALDETATSEQIQQAVEEVFPNSAPPPPSSGN
                     SGDTQNLDQPGKVNKESPRQRRSASGSTSRPNEADVLSPPGVEKKSAELRLDEHLKST
                     DRAEKSRSQSRRTSGAKAALAQRRGRYTRAVTFRSTGARIAIDATLRALVGLDSSLRD
                     GSLAPVDSEALRYKLLKHKQGRLFVFAIDASGSMAANRIARAKSTILKLLRKSYLNRD
                     SVAIVSFHGTTANLDLPPSRSILRARRVLDSLRMGGSTPLGLGLVTTIELLELVGNKF
                     GETVVLLFTDGRSNVPLRRGGLNLRAFRQVKIESELRELTVALKRTKARVVVVDTQKE
                     FESTEETRRLAWILRAGFVKIAPSNP"
BASE COUNT          599 a          820 c          742 g          654 t
ORIGIN      
        1 cccgcggcta tttgcttcca acagtcgctg cgtcaacgag tgtgccgaat gcggattcaa
       61 atcgcggagc cgctcaagca tgttctcatc aaacaggaac gtgttcgcca cctccgagta
      121 gatccaatcg tcaactgcat ctgccgtcgc actccatccg aaagtatttg aaacgtgatt
      181 ctcaatctca gccacgcctc ggaacccgtg cttcaacatc ccttcatacc acttcggatt
      241 cagagtcttg gcgcgcgtct ccagacgaac cgtctcttcc aacgatcgaa tcttcacctc
      301 acgcgataac gtgtcagaca aatagattgc gggccgcgac ttcgcacgct tctcaactgc
      361 tttcgacacg ccgccgagat actcaaagta gtggtctacg tcggtgaggc cgatctcaaa
      421 tgtatcgatg ttctggtagg ttgcctcgac acgtgaaagc gcgcggtcca ttgcaactcg
      481 cgcttccctg ccctcgatcg cacgtccctt ggaatcgcgg ccgtaagcaa aacacttgcg
      541 cgtcacaaac agatcgccaa gcgtctcttc acgctcccac tgtgaatcca tcaccatgaa
      601 attcacattc gtgccgtaat tgcccggcgc attcgaaaat acacgcaccg ccgcatcatc
      661 cagatcacaa gcttcctctt caacctgctg taagacgtgt ttgcgaacgt agttcagttc
      721 caaaggctcg tcgagttgag caaccactcg tacagcctta tcgagcagtt gtaccgttgg
      781 actaaacaga tcgcgaaaga ttcctgatac cgtcatgacg acgtcgattc gcggtcggcc
      841 gagttcactt aagggaatga cttcgacgct cgtcgtccta tttaaagcat cgcgaacggg
      901 gcgtgtgccg agcagccaca gagcctgagc cacgccttca ccctgagttt tgatgttgtc
      961 gagaccccac agcacgagcg ccattgcttc cgggtaacgc ccattttcct gctggtatcg
     1021 agaaagcaga ctgtttgcga ccgcttcact gcgcgcaaag gccagtgcgg aaggcacgcc
     1081 atagggattt agagcgtgag tattacgacc agtcgggagg atgtcggggt tctgaatgat
     1141 atcggcgcct ggtccaggtt cgatgaattc accacgcagc gcgcgcacga gtgaatcgat
     1201 ctcgttgttg gtttcaagct ttgcggtaac agtttcaagg agagcaaaaa cagggcgggt
     1261 tgcttccgag tcgagtcctt tcgtttgcag aaaagcggtc gcggcttcag ttccgtctgc
     1321 gacaaaggcg cgtacggctt cgtcgattag atcttcagga agcgcgcgca gtcctgcctc
     1381 gggacgatcg aatgaagcga tcatcttcag caggtcggtt ttctcacccg cagatgacgc
     1441 gcggccaaag acgtggaggc cggttgggat caatcgttgt tcgatctcaa ctagctgctc
     1501 gtaaagtgcg aacactttcg agtcacgttc attgtttggg ttattagcca cggattttac
     1561 ggattggacg gagcgatctt cacgaatcca gcgcgcagga tccacgccag acggcgcgtc
     1621 tcctcggtgg attcaaactc cttctgcgta tcgaccacca caacacgagc ttttgttctc
     1681 ttcagcgcca ctgtcagttc acgcaattct gattcgatct tcacttggcg aaatgcacgc
     1741 aggttcaggc ccccgcgtct gagcggtacg ttcgaacggc cgtcggtaaa caggagtaca
     1801 accgtctcgc caaatttgtt tcctaccagt tccaacaact caatcgtcgt taccaggcct
     1861 aaaccgagcg gcgtgctgcc acccatgcgc agcgagtcga gcacacggcg cgcgcgcaga
     1921 atacttcgcg acggcggcag atcgagatta gcggtggtgc catgaaacga aacgatcgca
     1981 acgctgtcgc gattcaggta cgacttgcgg agcagtttaa gaattgtgct tttcgcgcgg
     2041 gcgattcgat ttgcagccat gctgcccgac gcgtcgatcg caaacacaaa gagccggccc
     2101 tgtttgtgtt tcagcaactt gtagcgcaac gcctcggagt cgaccggcgc caaacttcca
     2161 tcgcgcaatg acgaatccag gccgacgagc gcacgcaggg ttgcgtcgat ggcgatgcga
     2221 gctccggtgc ttctaaatgt cactgcgcgc gtgtagcgcc cgcgtcgttg ggccaacgca
     2281 gccttcgctc cgctcgtgcg ccggctttgt gaacgagatt tctctgcacg gtcggttgac
     2341 ttcagatgtt cgtccagtcg caactcagcg gacttctttt caactccggg tggcgacaaa
     2401 acatctgctt catttggtct cgaagtactt ccggatgccg atcggcgttg gcgtggagac
     2461 tctttgttca ccttcccagg ttgatcgaga ttttgagtgt cgccgctatt gccgctcgac
     2521 ggcggtggcg gggcagagtt tggaaaaact tcttcaactg cctgttgaat ctgttcgctg
     2581 gttgcagttt catccagcgc gtcacgacgc aaacggtgtc gcagcgccat cgccgaaact
     2641 cttttcacgt gttcctcggt gacagatttg cgaccctcga acgcggccaa cgcacgtgca
     2701 gaacgcatga tcgtcagttc accacgatgg ccatcgactt tgagatcagc gcagagttgt
     2761 gcgatctttt cgaggacctt ccgatccata tttatcttcg ccaggttcgc acgcg
//
LOCUS       DASSFQ010000312         8616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_146583, whole genome shotgun sequence.
ACCESSION   DASSFQ010000312 DASSFQ010000000
VERSION     DASSFQ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146583"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2464)
                     /locus_tag="VFH96_11210"
     CDS             complement(<1..2464)
                     /locus_tag="VFH96_11210"
                     /inference="COORDINATES: protein motif:HMM:NF045064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974587.1"
                     /translation="MNEIWLGPLLNDNRAQLIERCAKFVANNQSDAFIYLASSYPLLE
                     QVTQKLLDGDRNCGVWGELPVYLFRGLVRRVLYSAVDADGHGLPPRIPIDRDELPLKR
                     SLVSQLLMQLLATNQLKAFAPLAMREGCVNSISTLLGEIQRAARTPAELADILAERAR
                     DVEKGKTEPRGVTQLDFDKEISLIYSNYAELLNQNLFTEEDAEQLRALWVLRGMIDEK
                     FVRVPWLANVQLLVIDGFFDFTPVQGEILRNLIPQLPQTIVNLNHDDSNPEIFAPFQE
                     TIGQLESIAKFDTVHSRDYTATSGVLATLRENLFNPLRSSDEISDDPESLVREIRYLE
                     CGDRDTEIRTIAREVKRLVLTENYDLSEIALVVRQRATYGPTITRVMLEESLPCNLEL
                     RIEAGDVPANRAALKLLLLLEGLSTDETQTTRIGEIADLIKSEYFRLSDVETNVLSTR
                     FDEHHLDLLRADLPKLTPDAVARLKNRHRIGFWDADSLENAFAYVGSDLNVSAWLARA
                     FKLYTDLPGAIATKELLNIDPGAHDRDTDIADNVENAETAKLEEKGVEKKRRPSRDIH
                     PAALAWTALVVQRFAELLHAVPREGKPTELRTELMRLLDRFGFRDQIAEPAKQATDEQ
                     QLPQVMLNFNALEALRRAFIAAIKSIELAGKSATVRLPVFLQEVRRSLTSQSQIFGAP
                     DRNGLRVLEATDVRGLRIRAVFIAGLTEGGFPLSASRDWIYPHEERDRLKEYGLTLED
                     ISPNTLLKEEHYFYQVACRATERLYLTRPLMLGDDSETVASYYIDELRRAIAPLRIET
                     EIIRRDYDGKNLDDASTTTEMKV"
     gene            complement(2524..3864)
                     /gene="rmuC"
                     /locus_tag="VFH96_11215"
     CDS             complement(2524..3864)
                     /gene="rmuC"
                     /locus_tag="VFH96_11215"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET6974588.1"
                     /translation="MEIVFPVSMLGIGLIVGTVSVWLITRTKLKYEFDRGRAEGETQR
                     ARLETRLEEERKASQEKLTLLNNAEARLADAFKALSADALRSNNQSFLDLAKQNLETF
                     QQTAKGELERRQSAIGDLIKPLKESLEKVDGKIGELEKNRVGAYTELREQVKQLAQSH
                     TQLQSETGNLVKALRVPHVRGRWGEIQLRRVVELAGMLQYCDFTEQETVATEDSRIRP
                     DMIVRLPGNRTIVVDSKVPFDAFYESISTTDEDVRAARLKDHARLVRTHITALSRKSY
                     WETVQPTPEFVLLFLPGETFYSAALENDPKLIEDGVSQGVIIATPTTLIALLKAVSYG
                     WRQEQMADNAQEVSDLAKTLYDRLRVFTSHFDDVGRNLDRALESYNRGVRSLESRVLV
                     TARRFKERGAIAGEEIEILEPIDKAARPLSRDDGGLFPEEDMPLLKARSAKAQR"
     gene            complement(4030..5061)
                     /locus_tag="VFH96_11220"
     CDS             complement(4030..5061)
                     /locus_tag="VFH96_11220"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET6974589.1"
                     /translation="MRGNVPIPEIPHGEDLWLIVLVTSVRERRTQQGRLFYDASARNA
                     TGSIALKIWGEIIETQKELKPGLWGITGKLETFQDRSQFVVAEYRPITIEQYREHQDA
                     EPQLPRAYTFDIETLTLPDFRERVGPQLERSMRLGNMRVEQQQRYLEDIAAEEERCYA
                     LGSLNATSGRILSIALHVASIPGLEFPGLGPEASEHVFGIDKYGHEEDEKEALQGFLT
                     FMKDFDPDCDELVGHNIIGFDLPCVFQRCLVHSLKARPFVNLADYNVRGVFDTMHRWW
                     LGARRHVSLDDVAWALGIESSKTAEVEGSKVFDLYQAGRLDLIREYNLNDVRVTRKVY
                     ERMVASFGR"
     gene            complement(5074..5403)
                     /locus_tag="VFH96_11225"
     CDS             complement(5074..5403)
                     /locus_tag="VFH96_11225"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HET6974590.1"
                     /translation="MSETRYKSFSEFWPFYVSEHSKPATRLLHLIGTTAGLALLVYLI
                     ATGRWWLFFLGFVPGYGFAWLAHFLIEKNKPATFQYPLWSFMGDYKMIALMLTGRMNQ
                     ELERLSR"
     gene            complement(5400..6503)
                     /locus_tag="VFH96_11230"
     CDS             complement(5400..6503)
                     /locus_tag="VFH96_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6974591.1"
                     /translation="MSTNCATTDGTARYIDRFAGRAANGHFREAQGLALSSIGIGTYL
                     GDADEATDQNYTNAVIRAAQLGVNVIDSAANYRFQRSERSIGHALKTLTNEHGFERDE
                     LLICTKGGYLPFDGEPPRNVREYVNETFVKPGIASFDDFVAGSHCMTPSYLQNQLDQS
                     LHNTGLECVDVYYIHNPESQLGHVSESEFYHRITIAFERLETNRQQGKLAYYGVATWN
                     GFRATADSENHHSLVRMYEIASAIGGDEHGFRFIQLPFNLAMPEALSLANEKIDGEPV
                     TVLEAAFRLGMSVMVSASMLQGRVARGLPDNIREALGSLPTDAQTAIQFVRSTPGITT
                     ALVGMSSVEHVDENLALAEKEPAGPEAFAALWE"
     gene            6552..8048
                     /locus_tag="VFH96_11235"
     CDS             6552..8048
                     /locus_tag="VFH96_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET6974592.1"
                     /translation="MPLRTQSSLDVSEEDLREISSQATKLVFEYLTTISERSVRAENH
                     AGKTLRSIAAELSSEGLPLARLLDECRTIMDLSRHNGHPRFFGYVASPSTPIGAYGDL
                     IASALNANITCWRSGPAGTELERMVVRWLGSLINYDQDAKGLLTSGGSMANLIALLVA
                     NRRKSGAETSQRGLWNSGSPMTVYASSEVHMSIAKAADILGFGRDYVREIPCDERQRM
                     RVDLLREKISVDLSEGLRPFCVVGSAGTVNTGVVDPLSEIAAVATEFDLWFHVDGAYG
                     APGVLDERKQHLFAGLERADSVSLDPHKWLYVPVDAGCLLFRDDAAAKATFSTEEADY
                     IKTHGYVDEEAFAFWDYGVELSRRFRALKVWLTLQYYGSRRIAAAISDDISLAEYFGE
                     VVSNANDFELLAPVELSICCFRYIPRERPSETELDKLNERIMERVQKGGRAYLSNATV
                     NGHFALRACITNFRTTKNDIDETVAAIREAAEDLTQRRKDGTEDAKDL"
     gene            8106..>8616
                     /locus_tag="VFH96_11240"
     CDS             8106..>8616
                     /locus_tag="VFH96_11240"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET6974593.1"
                     /translation="MTTPFDHELRIACELARTAGAAILEQYEGPLNIRQKNYDDDVEP
                     VTQADTIANELIVTGLKREFPDDGILAEESVDTERRLAKSRVWMVDPLDGTNGFIDGN
                     GDFAVQIGLAEDGRCVLGVVYQPLSGVLYRAVRGTGTWIERSQFEPEKATVSDTRALS
                     EMRLAASRSH"
BASE COUNT         1832 a         2423 c         2415 g         1946 t
ORIGIN      
        1 cgactttcat ctcggtggtc gtcgaagcgt cgtcgaggtt cttgccgtcg taatctcgcc
       61 gaatgatctc agtttcgatt cgcagcggcg cgatcgcgcg tcgcagctcg tcgatgtagt
      121 acgaagcgac agtttccgaa tcgtcgccga gcattaacgg gcgcgtgaga tacaaacgct
      181 ccgtggctcg acacgcgacc tggtagaaat aatgctcttc ttttaggagc gtgtttggcg
      241 agatgtcttc gagcgtgagg ccgtactctt tgagtcgatc gcgctcttcg tgtggataaa
      301 tccaatcgcg tgacgcactc agcgggaaac cgccttcagt cagtccggcg atgaaaaccg
      361 cgcgaatccg cagaccgcgc acgtccgtcg cttcgagcac gcgcaggccg ttgcgatcgg
      421 gcgcaccgaa gatttgcgac tgcgaagtca gcgaccggcg cacttcctgc agaaacaccg
      481 gcagtctgac tgttgctgac tttccggcca gctcgatgct cttgattgcg gcgataaatg
      541 cgcgtcgcaa cgcttcaagt gcgttgaagt tgagcatcac ctgcggcagt tgctgttcgt
      601 cggtcgcttg tttcgctggt tccgcaattt gatcgcgaaa accaaaacga tccaatagcc
      661 gcatcaactc ggtgcgcagt tccgtgggct tgccttcgcg tggcactgcg tgcaacaact
      721 ccgcaaaacg ttggaccacg agcgcggtcc acgccaaagc cgcgggatga atatcgcgcg
      781 acgggcgacg cttcttctcg acgcctttct cttccagctt tgcggtttcg gcgttctcga
      841 cgttgtcggc gatgtcagtg tcgcgatcgt gtgcgcccgg atcgatgttc agcagttcct
      901 tcgtcgcgat cgcgccggga agatccgtgt acaacttgaa tgcacgcgcc agccaggcac
      961 tgacgttcaa atcactgccg acgtatgcaa aagcattctc gagcgaatca gcgtcccaga
     1021 aaccgatgcg atgccgattc tttagtcgcg cgactgcatc aggcgttaat ttcggtaggt
     1081 cggcccgcaa gagatcgagg tgatgctcat cgaagcgcgt cgaaagcaca ttcgtttcga
     1141 cgtcgctgag ccggaagtat tccgacttga tcagatccgc aatctcacca atccgcgttg
     1201 tctgtgtttc gtcggtcgaa agtccttcga ggagtaaaag aagtttgagt gcggcgcgat
     1261 tcgcgggaac gtcgccggcc tcaatacgca actccagatt gcatggcaac gactcttcaa
     1321 gcatcacgcg cgtgatcgtc ggtccatacg ttgcgcgttg acgaaccacc aatgcgatct
     1381 cggaaagatc gtaattctca gtcagcacga ggcgttttac ttcgcgcgcg atggtacgaa
     1441 tctctgtgtc gcgatcgccg cactcgagat aacgaatctc gcgcacgagc gactccggat
     1501 catctgaaat ctcgtccgag gatctcagcg gattgaagag gttttcacgc aaagtcgcta
     1561 agacgccgct ggtcgccgtg taatcgcgcg aatgcaccgt gtcgaactta gcgatgcttt
     1621 cgagctgtcc gattgtttcc tgaaacggcg cgaagatctc gggattgctg tcgtcgtgat
     1681 tgagattgac gatcgtttgc ggcaactgcg gaatcaggtt gcgcaggatc tcgccctgca
     1741 ccggcgtaaa gtcgaaaaag ccgtcgatta cgagcagttg cacattcgcc agccacggca
     1801 cgcgcacgaa tttctcatcg atcatgccgc gcaaaaccca aagcgcgcgc agttgctccg
     1861 cgtcttcttc ggtgaacaaa ttctgattca gcagctcggc gtaattcgaa tagatcagcg
     1921 aaatctcttt gtcgaaatcg agctgagtga cgccgcgtgg ctcggtctta cctttttcca
     1981 cgtcgcgggc acgctccgcg agtatgtcgg cgagctccgc cggcgttcgt gccgcacgct
     2041 gaatctcgcc aagcaaagtc gagatactgt tcacacaacc ttcgcgcatc gcgagcggcg
     2101 caaaggcttt caactgattc gtcgcgagca actgcatgag cagctgcgaa accaaactgc
     2161 gttttaacgg caactcatcg cgatcgatcg gaattcgtgg cggcagtccg tgaccatccg
     2221 catcgacggc cgaatagaga acgcgtcgca cgagtccacg aaacaaataa accggaagct
     2281 caccccaaac accgcaattg cgatcgccgt ctagcagctt ttgtgtgacc tgttcgagga
     2341 gtggatacga cgaggcgagg tagatgaacg cgtcgctttg attgttggct acgaatttag
     2401 cgcagcgctc gatgagttgc gcgcgattgt cgttgagtag aggccctaac cagatctcgt
     2461 tcacagcagt atccacagat tacacagatt atgccgatct gcgaaatctg cgaaatctgt
     2521 ggtttagcgt tgcgccttgg ccgatcgggc cttgagcaac ggcatatctt cttcgggaaa
     2581 cagtccgccg tcgtcgcggc tgagcggtcg ggcggccttg tcgatgggct cgagtatttc
     2641 gatttcttca ccggcaatcg cgccacgttc tttgaaacgc cgcgccgtta ccagcacgcg
     2701 agattcaagt gaacgcacgc ctcgattgta ggattcgagt gcgcgatcga ggtttcgccc
     2761 gacgtcgtcg aaatgactgg tgaacacgcg cagtcgatcg tagagcgtct ttgcgaggtc
     2821 gctcacctcc tgcgcgttgt cagccatctg ctcctgacgc caaccgtatg acaccgcctt
     2881 caacaacgcg atcaaggtcg tcggagtcgc gatgatcact ccctggctga cgccatcttc
     2941 gatcagtttc ggatcgtttt ccagtgcagc actgtaaaac gtttcgccgg gaagaaatag
     3001 cagcacaaac tcgggcgtcg gttgcactgt ttcccagtac gacttccggc tgagcgccgt
     3061 aatgtgagtt ctcaccaatc gtgcgtggtc cttcaacctc gccgcccgga catcttcgtc
     3121 agtcgtcgag atcgattcgt agaacgcgtc aaacggcact ttcgaatcaa cgacgatcgt
     3181 gcgattgccc ggcagacgca cgatcatgtc gggacgaatt cggctgtctt ccgttgcgac
     3241 cgtttcctgt tcggtgaagt cgcaatactg cagcatgccg gccagctcga ccacgcgccg
     3301 caactgaatc tcaccccagc gaccacgaac atgcggcacg cgcaacgctt tgacgaggtt
     3361 tccagtctcc gattgcagct gagtatgcga ctgcgcgagc tgcttcacct gttcgcgcaa
     3421 ctccgtgtac gcaccaacgc gattcttttc gagctcaccg atcttgccgt caactttttc
     3481 gagcgattcc ttgagtggct tgattagatc gccgatagca ctctggcgac gctcgagttc
     3541 gccttttgca gtttgctgaa acgtttcgag attctgtttt gcaagatcga ggaacgactg
     3601 gttattgctg cgcagagcgt cggctgaaag cgctttgaac gcgtctgcga gtctggcttc
     3661 ggcattattc aatagcgtga gcttttcctg gctcgctttc cgttcttctt cgagacgagt
     3721 ttcgagacgc gcacgctggg tctcaccttc agcccggccg cggtcaaact cgtatttaag
     3781 ctttgttctg gttatgagcc agacggagac ggtgccgacg atgagcccga tgcccagcat
     3841 cgacacgggg aagacaattt ccatggaacg tcattctatc actcgcgcgt gttcatatcc
     3901 atatcttgtt tgtctgagca ccggtgaccc caattagctg tgctgttcgg aggagttttc
     3961 tcacgcaaag gcgcaaagcc ttcggtttct ttgcgtcttc gttacacctt tgcgcctttg
     4021 cgtgagaaat caccttccga aacttgcgac catgcgttcg tagactttgc gcgtcacgcg
     4081 cacgtcgttg aggttgtatt cgcggatcaa atcgaggcgg ccggcctgat aaagatcgaa
     4141 aactttactg ccttcgacct cggcggtctt gctcgactcg atgcccaacg cccaggcaac
     4201 gtcgtcgaga ctaacgtgac gtcgcgcgcc gagccaccag cgatgcatcg tgtcgaaaac
     4261 accacgtacg ttgtaatcag cgagattcac gaacggtcgc gctttgagcg agtggacgag
     4321 acagcgctga aacacacacg gcaggtcgaa accgatgatg ttgtgtccga caagctcatc
     4381 gcagtcggga tcgaaatcct tcatgaaggt cagaaaaccc tggagcgctt ccttttcatc
     4441 ttcttcatga ccgtacttgt cgataccaaa gacgtgttcg ctggcttcgg gaccaagacc
     4501 gggaaactcc agacctggaa tcgaagcgac atgaagcgcg atcgagagaa tgcgccccga
     4561 agtcgcattt aacgacccga gcgcgtaaca acgctcttcc tcggcggcga tgtcttcgag
     4621 atagcgttgc tgttgctcga cgcgcatgtt gccgagtcgc atcgagcgtt cgagttgtgg
     4681 cccgacgcgc tcgcggaaat caggcaaggt cagtgtttcg atgtcgaaag tgtatgcgcg
     4741 cgggagttgt ggttcggcgt cttggtgttc gcgatattgt tcgatcgtga ttggacgata
     4801 ttcggcgacg acgaattgtg agcgatcctg aaatgtttcg agtttgccgg tgatccccca
     4861 aagccccggc ttcaattctt tttgagtttc aatgatctcg ccccagatct tcagcgcgat
     4921 cgacccggtg gcattgcgcg cggaagcgtc gtagaacaaa cggccctgct gtgtgcgccg
     4981 ctcgcgcacg gacgtcacga gaacgatgag ccacagatcc tcgccgtggg gaatttcggg
     5041 aatgggaacg ttgccgcgca ttaaatagtg gcgctatcgg gaaagcctct ctaactcctg
     5101 attcatccgc ccggtcagca tcaacgcaat catcttgtaa tcgcccatga acgaccagag
     5161 cggatactga aacgtggccg gcttgttctt ttcgatgagg aagtgcgcga gccacgcgaa
     5221 gccgtaaccc ggaacaaacc cgagaaagaa caaccaccac cgtccggttg cgatcaaata
     5281 aaccagcagc gcgaggccag ccgtcgtgcc gatcaaatgg agcagacgcg tcgcgggctt
     5341 gctgtgttcc gacacataaa acggccagaa ctcggaaaat gatttgtatc gcgtttcgct
     5401 cattcccata acgctgcaaa agcttcgggt ccagccggtt ctttctcggc cagggcgaga
     5461 ttttcatcta cgtgctcgac gctgctcata ccgacgagcg ccgtcgtgat tcccggcgtc
     5521 gagcgcacga attgaatcgc cgtttgcgcg tcggttggca acgatccgag tgcttcacga
     5581 atgttgtcgg gcagtccgcg cgccacccga ccctgcaaca tcgaagcgct caccatcact
     5641 gacatcccta atctgaacgc tgcctccaga accgtgaccg gctcgccgtc gattttctcg
     5701 ttcgcgagcg atagcgcttc gggcatcgcg agattgaaag gcagttgaat aaatcgaaag
     5761 ccgtgctcgt cgccgccaat cgcgctcgcg atctcataca tgcgcacgag tgaatgatga
     5821 ttttccgaat cggcagtggc gcgaaatcca ttccaggttg cgacaccata ataggcgagc
     5881 ttgccttgct gtcgattcgt ttccagacgt tcaaacgcaa tcgtgatccg gtgataaaac
     5941 tccgactcag aaacatgtcc gagctgcgac tccggattgt gaatgtaata aacgtcaaca
     6001 cactctagcc ccgtgttgtg gagcgactga tcgagttgat tctgcaagta cgacggcgtc
     6061 atgcaatgac tgccggcgac gaagtcatcg aacgaagcga ttcccggctt tacgaaagtc
     6121 tcgttcacgt attcgcgcac attgcgcggc ggttcgccat cgaacggtaa ataaccacct
     6181 ttggtgcaaa ttaagagttc atcgcgttca aacccatgct cgtttgtcag agtctttaac
     6241 gcatggccaa tcgagcgttc actccgttga aaacgataat tcgcggcgct gtcgatgacg
     6301 ttcacgccga gctgcgccgc gcggatgacg gcattcgtgt agttctggtc agtcgcttcg
     6361 tcggcgtctc cgagataagt gccgatgccg atcgatgaga gcgcgaggcc ctgcgcttcg
     6421 cggaagtgtc cgttcgcggc gcgacccgca aaacggtcga tataacgggc tgtgccgtca
     6481 gtagtggcgc aattcgtcga catattgagc gaatggtaac acgaagagcc ttatgttagt
     6541 ttgtgctcac aatgccactg cgaacgcaat cctccctcga cgtcagcgag gaagatcttc
     6601 gggagatctc gtctcaagct acgaagctgg tcttcgagta tttgacgacg atctcggagc
     6661 gctccgttcg cgctgaaaat catgctggta aaacgcttcg atcgatcgcg gcggagctct
     6721 cgagcgaagg tttgccactc gcgcgattgc tcgacgagtg tcgcacgatc atggatctca
     6781 gccggcacaa tggtcatccg cgcttctttg gttatgtcgc ttcgccgtcc actccaattg
     6841 gcgcctacgg ggatctgatt gcttccgcac tcaacgcaaa tatcacctgc tggcgttccg
     6901 gaccggccgg cacggaactc gaacgcatgg tggtgcggtg gctcggttcg ctcattaatt
     6961 acgatcaaga cgcgaaggga ttgctgacca gcggtggctc gatggctaac ttgatcgcgt
     7021 tgctcgtggc taatcgtcgc aagtcaggcg ctgaaacatc gcaacgcgga ctctggaact
     7081 cgggctcgcc aatgactgtt tacgcgtctt cggaagttca catgtcgatc gcgaaagcgg
     7141 ctgacattct tggcttcgga cgcgattacg tgcgcgagat tccgtgtgat gagcgccagc
     7201 gcatgcgtgt cgatctgttg cgtgaaaaga tcagcgtcga tctgagtgaa ggactgcgac
     7261 ccttttgcgt cgtcggcagt gccggcactg ttaacacggg cgtagtcgat ccgctatcag
     7321 aaatcgccgc cgtcgcgact gagttcgatc tctggtttca cgttgacggt gcttatgggg
     7381 ctcccggcgt tctcgatgaa cgaaagcagc atctcttcgc cggactcgaa cgagcggatt
     7441 ccgtctctct cgatccgcac aaatggcttt acgttccggt ggacgctggt tgtcttctct
     7501 tccgcgacga tgcggcagcg aaggcaacgt tcagcacgga agaagctgat tacatcaaga
     7561 cgcacggata cgtggacgaa gaggcgtttg cgttctggga ttatggagtt gagctgtcgc
     7621 gacgctttcg cgcgctcaag gtttggttaa cgttgcagta ttacggcagt cgacgcattg
     7681 cggcagcgat cagcgacgac atctcgcttg cggagtattt tggagaggtg gtgtcgaacg
     7741 cgaacgactt cgaactgctc gcgccggtgg aattgagcat ctgctgcttt cgttacattc
     7801 cacgcgagcg accgagcgag accgaattgg ataagcttaa cgaacgcatc atggagcgcg
     7861 tgcagaaagg cggccgcgcg tatttgtcga atgcgactgt gaacgggcat ttcgcgctgc
     7921 gggcctgcat tacaaacttt cggacgacga agaacgatat cgatgagact gtcgcggcaa
     7981 ttagagaagc cgcggaagat ctcacgcaaa ggcgcaaaga tggaacggaa gacgcaaagg
     8041 atttgtaagt agtttttttg cgccttcgac ttcacctttg cgcctttgcg tgaaacctat
     8101 tcgaaatgac cacacccttt gaccacgaac tgcgcatcgc ctgcgaactc gcacggacgg
     8161 ccggcgctgc gattctcgaa cagtacgaag gcccgctcaa tatcagacaa aagaattacg
     8221 acgacgacgt tgagccagtc acgcaggccg acacgatcgc gaatgagctg atcgtcaccg
     8281 gcttgaaacg tgagtttcca gacgacggca tcctcgcgga agagtcagtc gataccgaaa
     8341 gacgcctcgc aaagtcgcgc gtctggatgg tcgatccact cgacggcacg aatggcttca
     8401 tcgacggcaa cggcgatttt gcagttcaga tcggactcgc ggaagatggt cgctgtgttt
     8461 tgggagtggt ctatcaacca ctcagcggcg tgctgtatcg cgccgtgcgc ggcacgggta
     8521 catggatcga acgttcgcaa ttcgagccgg agaaggcaac cgtctctgac acgcgagcgc
     8581 tctccgagat gcggctcgcc gccagccgat cgcacc
//
LOCUS       DASSFQ010000313         6425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1339024, whole genome shotgun sequence.
ACCESSION   DASSFQ010000313 DASSFQ010000000
VERSION     DASSFQ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339024"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2435
                     /locus_tag="VFH96_11245"
     CDS             <1..2435
                     /locus_tag="VFH96_11245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6974594.1"
                     /translation="LFTNVGIGSWLLLLAIAIASSPLTVRVASDDGILRSRESIADSF
                     VLLAVMLYAVPPSNTAGPAVLLTAVVAFVSTYRLATNREVVLKTGMAVVSTFIAASLY
                     GALIDLFAGQSELPAQGALPLNVFLIPLLVVAALQYALSTIATAWFISFEAGKFTLVP
                     TPETVVWTLTTKLAGAASASLFYAAVLNKTLAYAVLGLLISLLVYLLYRFNERRLEQI
                     RDAEAERRRHVEEMAEIHMNTIESLAIAIDAKDQTTHGHVRRTQLYATQMGKLFNVSE
                     SELRALHAGALLHDIGKLAVPEYILNKPGKLTEAEFAKMKIHPVVGGDILKRVNFPYP
                     VEDIVRYHHEKWDGSGYPKGLKAEGIPLVARIISVVDFYDATRCDRPYRKGMKREESL
                     ALLRSMVGNAFDPKVVDMFVQHVEEFDRMIDLQDIKEQVASAATEDTQTATKPDAGLA
                     PDILGVAEEGSPFRSISDAQREVFALHEIAQTIGSSLNLSDTVTLVANKLRAIVPFDT
                     CVIYLVDDPSGKAIAAHVLGEEVEVFKKRRINIGDGITGWVIANARSMCNASPDLDLI
                     GIPDEIVKRYRGVLVSPLQREDGAFGAISLYSQSRTSYTTEHVRLLESVCQHASSALN
                     NALTYEKTRDSALIDPLTELPNARGFYMMLEQRIAECQRMNREPVAVVCMDIDDFKIV
                     NDTYGHSIGDRLLASVAGVVRRELRQMDILTRYAGDEFVAIMPMASSKMAISISERMR
                     NAVEEQLFSVRTGTMVGLGVSLGVACFPDDGETSEELLSAAARRMQTDKNSRKAIITV
                     AGAGVSSIDMMT"
     gene            2693..3619
                     /locus_tag="VFH96_11250"
     CDS             2693..3619
                     /locus_tag="VFH96_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974595.1"
                     /translation="MFSKSSLVRTSILLFALVLTSRVEAIELTGGRVSRTGFVSPAAF
                     SLRGPGLSYTGSFDTNMWFVPCTPCFPGDLQSTSAHLVLSTVDFSLSTSITIDGKTFF
                     NWGSFSNPPPPTAIFTSSMDFLGGSVEIPHSDEPELTLFAPFSMSGVVGGRGVSSQLF
                     LVELDASGFASLNLKRIDSSNRPAYLFQAITYEIATRVDIDVKPGDDRNFINPRSHGK
                     TPISILSTTVFDAATVDPLTIRVAGAPINVKKNGTTASSLEDVNGDGLLDLVVHVSTE
                     ALQPTSPNQVLLEAFTTSGERLWGIAEIILHP"
     gene            3746..4885
                     /gene="pdhA"
                     /locus_tag="VFH96_11255"
     CDS             3746..4885
                     /gene="pdhA"
                     /locus_tag="VFH96_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET6974596.1"
                     /translation="MSKQTAAPPSAAKAEEQQSSTGAGVIDAPQQVDPQLLKKERDQA
                     KERQKDRLSGLDKDQLVELYRQMLLIRRFEEKSAEVYVAGKIGGFCHLYIGQEAVGVG
                     TISAIRKDDYVLASYREHGLALAKGMSARAIMAELFGKATGCSKGKGGSMHMFDKSLG
                     FLGGHAIVGGQIPLATGTAFATKYQGTDQVTLCYFGEAAVNQGAFHESLNMAQLWKLP
                     CIYICENNQYGMGTSLARAMSLQNIAQKACAYNMASEFVDGMDVLAVREATLRAVERA
                     RKEYLPTLLEIRTYRFMGHSMSDPGNYRTRAEIEKYQERDPIKLFSASLLEEKIVDQK
                     TLDEIDRKIHEEVEDSLRFADESPLPDPKELYTDVYANPIEPGRK"
     gene            4895..5878
                     /locus_tag="VFH96_11260"
     CDS             4895..5878
                     /locus_tag="VFH96_11260"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF008854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HET6974597.1"
                     /translation="MAIITIREALNQALREELARDEKVFLMGEEVAEYDGAYKVTKGL
                     LHEFGNKRVIDTPITELGFAGLGVGAAMAGLRPVIEFMTFNFSILATDQIINSAAKML
                     YMSGGQFNIPIVFRGPSGSAFQVSSQHSQAIESWYAYFPGLKVVMPSTPADAKGLLKS
                     AIRDDDPVIFMEQERMYGQKGEVPDDPDFTIPLGVADVKREGTDVTIVARSLTVPLAL
                     KAAEQLQEQGVSCEVIDPRTIRPLDIDTIVASVKKTNRVVIAEESHPICGVGAEISAE
                     IMERAFDYLDAPVKRVSGVDVPMPYAKNLENLAIPDVNRIVAAVREVAYLD"
     gene            5904..>6425
                     /locus_tag="VFH96_11265"
     CDS             5904..>6425
                     /locus_tag="VFH96_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET6974598.1"
                     /translation="MATKVIMPKLSPTMEEGQISRWLKKEGDKVSMGEPLAEIDTDKA
                     TMEMQALGNGVLRKILIGEGQSAPLGQLIAVIGEPDEDIASLLSEAPAPPQPQEQKET
                     DEPPPAPDVTQPPAPNLAPANGRQPPAATDSGRMIVSPLAARMAAEAGIDLRSLQGSG
                     PGGRIIKRDIEAVI"
BASE COUNT         1458 a         1802 c         1787 g         1378 t
ORIGIN      
        1 cattattcac gaacgttggt attggatcct ggttactgct gctcgcgatc gcgatcgctt
       61 cgagcccgct gacagtccgt gtggccagcg acgacggcat acttcgcagc cgcgaatcga
      121 ttgcagattc gttcgtgctg ctcgcggtca tgctctacgc cgtgccgccg tcaaacaccg
      181 ctggaccagc agtgctgctc accgcggtcg tcgctttcgt ttccacttat cgccttgcca
      241 cgaatcgaga agtcgtgctc aagaccggga tggccgtggt ctccacgttt atcgctgcct
      301 cgctgtacgg tgccctcatc gatctttttg ccgggcagag tgaactgccg gcacagggcg
      361 cactgccatt gaacgtcttc ctgatcccgc tactcgtcgt cgctgcactc caatacgctc
      421 tcagcacgat cgccactgcc tggtttatca gcttcgaagc gggcaagttc acactcgttc
      481 cgactccgga aacagtcgtt tggacgctca ctacaaaact cgccggtgct gcgtcagctt
      541 ccctgttcta tgcggccgtt ctgaataaga cactcgcata cgccgtcctg ggtctattga
      601 tctctttact cgtctatctt ctctatcgct tcaacgagcg acgcctcgaa cagatccgcg
      661 atgctgaagc tgagcgacgc cgtcacgtcg aagagatggc tgagattcac atgaacacga
      721 tcgagtcgct cgcgattgcg atcgatgcga aggaccaaac gacgcacggc cacgttcgcc
      781 gcacacaact ttacgcaaca cagatgggta agctcttcaa cgtctccgaa tcggagttgc
      841 gcgcgctaca tgccggcgcg ttgctgcacg acatcggcaa actcgcggtg cctgagtaca
      901 tcctgaacaa gccgggcaaa ctcacggaag ccgagtttgc gaagatgaag atccatccgg
      961 tggtcggcgg cgacattctc aagcgcgtca atttcccgta tccggttgaa gacatcgttc
     1021 gttatcacca cgagaaatgg gacggcagcg gctatcccaa aggactcaag gccgaaggga
     1081 ttccactcgt cgcgcgcatc atctcggtag tcgatttcta cgatgcgaca cgttgcgatc
     1141 gtccttatcg caagggaatg aaacgcgaag agtcgctcgc actcctgcgc agcatggttg
     1201 gaaacgcgtt cgatccgaag gtggtcgaca tgtttgtgca gcacgtcgaa gagtttgatc
     1261 gaatgatcga cttgcaggac atcaaggagc aggttgcgtc cgcggcgacg gaagacacgc
     1321 aaacggccac gaaacctgac gcgggtctcg cgccggacat tctcggcgtt gctgaagaag
     1381 gctcgccgtt ccgatcgatc agtgacgcgc agagagaagt ttttgcgtta cacgagatcg
     1441 cgcagacgat cggctcgtcg ctcaatctca gtgacacggt cacgcttgtc gcgaacaagt
     1501 tgcgcgcgat cgtgccgttt gacacgtgcg tgatctatct cgtcgacgat ccatccggca
     1561 aagcgattgc ggcgcacgtt ctgggtgaag aagtcgaggt gtttaagaag cggcgtatca
     1621 atatcggaga tgggattacg ggttgggtga tcgccaatgc gcgttcgatg tgtaacgcgt
     1681 caccggatct cgatctgatc ggcattcccg atgaaatcgt gaagcgctat cgcggcgtgc
     1741 tggtgtcgcc gttgcagcgc gaagacggtg cgtttggcgc gatttcgctc tactcgcaga
     1801 gtcgcacgtc atacacgacc gagcacgtgc gtttgctgga atctgtttgc cagcacgcgt
     1861 cgagcgctct caacaatgct ttgacttacg agaagacgcg cgacagcgca ctcatcgatc
     1921 cgctgaccga gctgccaaac gctcgcggct tctacatgat gctcgaacag cgaatagcgg
     1981 agtgtcagcg aatgaatcgt gagccggtgg ccgtcgtctg tatggacatt gacgacttca
     2041 agatcgtgaa tgatacctat ggtcactcga ttggcgatcg gctgctggcg agcgtcgcgg
     2101 gcgttgttcg ccgcgagctc aggcagatgg acatcctgac tcgttatgcc ggcgacgagt
     2161 ttgtcgcgat catgccgatg gcgtcgagca agatggcgat ctcaatcagc gagcgcatgc
     2221 ggaatgcggt tgaagagcag cttttctctg tgcgcaccgg caccatggtt ggcctgggcg
     2281 tgagtctcgg cgtcgcctgc ttccctgacg acggcgagac gagcgaagag ctcttgagcg
     2341 ccgccgcacg ccggatgcag accgacaaga acagccgcaa agcgatcatc accgtcgccg
     2401 gcgcgggtgt cagctcgatc gacatgatga cttaaggcgc aaagccacac aaattccagc
     2461 cataaaaagg cacaaagccc tcatgtgagt gtcgtagaca cccattccac acacctgccc
     2521 ggaagaatca ctcaactgcc cccggagcca ctgtaatctc ccgtggtatg attgcggagc
     2581 ttgcatcatt aatgtgggct cgaaatggct gttgagttca ctcccgacaa ccaaaggctt
     2641 cgcccgccga acgagtctaa aacctcaacc tcaagcaaag gagaacaagg gaatgttttc
     2701 aaaatcttca ctcgtgcgca cctcaatctt gcttttcgct cttgtgctaa cttcgcgcgt
     2761 tgaagcgata gaactcactg gcggcagagt ttctcggacc ggttttgtca gccccgctgc
     2821 tttttcactc cgtggtccgg gactaagtta tacgggaagc ttcgatacta atatgtggtt
     2881 tgtgccgtgc acgccatgtt ttccgggcga tctacaatcc acgtcagccc atctcgtcct
     2941 gagcacagta gatttctcgt tatccacgtc aatcacaatc gatggaaaaa cgtttttcaa
     3001 ttggggatcg ttttccaatc cgcctccgcc taccgccata ttcacaagtt caatggattt
     3061 cttaggcggt tcggttgaaa ttcctcacag cgacgagcct gagttaactc tgttcgcgcc
     3121 cttctcgatg agtggcgttg tcggtggtcg gggtgtttct tctcagttgt ttcttgtcga
     3181 gttggatgca agcggattcg cttctttgaa cttgaaaagg atcgactcat caaatagacc
     3241 cgcttacctg ttccaggcta ttacctacga gatcgccacg cgagtcgata tagatgtcaa
     3301 acccggcgat gatcgcaatt tcatcaatcc aaggtctcac ggaaagacgc cgatatcgat
     3361 tctaagcacc acggttttcg atgccgctac ggtggatcct cttaccattc gcgtggcggg
     3421 tgctccaatt aatgtaaaga aaaacgggac gacagcttca tcactggaag atgtaaacgg
     3481 tgacgggctg cttgatcttg ttgttcacgt gagcacagaa gctctccaac caacgagtcc
     3541 caatcaggtt ttgttggaag cattcacgac ttctggagag cgtttgtggg gaatagccga
     3601 aatcatcctg catccgtaaa cgcgatttcc atgatgcaga gtttggcggg aggcgagatg
     3661 caaacagcac tcgcctcctt aaacttttcg tgctttttgc gcctttttgt gactaacgat
     3721 attttccctt ataatccgac gattcatgag caaacaaaca gcagcaccac cttcagccgc
     3781 taaagccgaa gagcagcaaa gctctacggg cgccggagtt attgacgcac ctcagcaagt
     3841 agacccccaa ctcctgaaaa aggaaagaga ccaggccaaa gaacgacaga aagatcgtct
     3901 cagcggtctc gacaaggatc aactcgtcga gctttaccgc cagatgcttc tgatccgtcg
     3961 cttcgaagag aaatcggcgg aagtttacgt cgccggtaag atcggcggct tctgtcacct
     4021 ctacatcggc caggaagcag tcggcgtggg cacgatttcc gctatcagaa aagacgacta
     4081 cgtgctcgcc agctatcgcg aacacggcct cgcgctcgcc aaagggatgt cagcccgagc
     4141 gatcatggcc gagctctttg gcaaggcgac cggctgctcg aaaggcaaag gcggctcgat
     4201 gcacatgttc gacaagagcc tcggctttct cggcggccac gcgatcgtcg gcggccagat
     4261 tccactcgcc accggcaccg ctttcgcgac gaaataccag ggcaccgatc aggtaacgct
     4321 ctgttacttc ggcgaagcag cagtcaacca gggcgcgttt cacgaatcgc tgaacatggc
     4381 ccagctctgg aaactgccgt gcatttatat ttgcgagaac aaccagtacg gcatgggcac
     4441 ttcccttgcc cgcgcgatgt ctctgcaaaa catcgcccaa aaagcctgcg catacaacat
     4501 ggcttccgag tttgtcgacg gcatggacgt acttgccgtt cgtgaggcga cgttgcgagc
     4561 agtcgagcgc gctcgcaaag aatacctgcc gacgctgctc gaaattcgca cctatcgctt
     4621 catgggccat tcgatgtctg accccggcaa ctatcgcacg cgagccgaaa tcgagaagta
     4681 ccaggagcgc gacccaataa aacttttctc tgcgagcctg ctggaagaaa agatcgtgga
     4741 ccagaaaacg ttggacgaaa tcgaccgcaa gatacatgaa gaggtcgaag attcactgcg
     4801 ttttgctgac gagagcccgc tgcccgatcc gaaggagctt tacacagacg tctacgcaaa
     4861 cccaattgaa ccaggccgca aatagaaccc gaacatggca ataatcacaa tccgcgaagc
     4921 acttaatcag gccctccgtg aagagctggc ccgagacgaa aaagtttttc tcatgggcga
     4981 ggaagtcgcc gagtacgacg gcgcttacaa agtaaccaaa ggcctcctgc atgagtttgg
     5041 caacaagcga gtcattgaca caccgatcac ggagctcggc ttcgccggtt taggtgttgg
     5101 cgctgcaatg gcagggctga gacccgtcat cgagttcatg acgttcaact tctcgatctt
     5161 agccaccgat cagatcatta actccgctgc gaagatgctt tacatgtcgg gcgggcagtt
     5221 caacattccg atcgtatttc gtggtccgag cggttcggcg tttcaggttt cgtcgcagca
     5281 ctcgcaggcg atcgaatcgt ggtacgcata ctttccggga ctgaaagtcg tgatgccgtc
     5341 gacgccggct gacgcgaagg gccttttgaa atcggcgatt cgcgatgacg atcctgtgat
     5401 ctttatggag caggagcgaa tgtacgggca aaagggcgag gtgccggacg atccggattt
     5461 cacgattccg ctcggcgtcg ctgacgtcaa acgagaagga accgacgtca cgatcgtggc
     5521 gcgctcgttg acggtaccgc ttgcgttgaa agccgcagag caacttcagg agcagggcgt
     5581 ttcctgcgag gtgatcgatc cacgcacgat tcggccactg gatatcgata cgattgtcgc
     5641 gtcagtgaag aagacaaacc gcgtcgtgat tgcggaggag tcgcacccga tctgcggcgt
     5701 gggcgcggag atcagcgcgg agatcatgga gcgtgcgttt gattatctcg acgcgccggt
     5761 gaagcgagtt tcgggtgttg acgtgccgat gccgtacgcg aagaatctcg agaatctcgc
     5821 gataccggat gtgaatcgca tcgtcgctgc agtgcgcgaa gtcgcgtatt tggattagtc
     5881 cttttccaca gattgcacag actatggcaa ccaaagtaat catgccgaag ctctccccga
     5941 cgatggagga gggacagatc tcgcgttggt tgaagaaaga gggagacaag gtctccatgg
     6001 gcgagccgct cgccgaaatc gacaccgaca aagcgacgat ggaaatgcag gcgctcggca
     6061 acggcgtgct gcgaaagatc cttatcggcg aaggacagtc ggcgccgctg gggcaactga
     6121 tcgccgtgat tggtgagccc gacgaagaca tcgcgtcgtt gttgtctgaa gcgccggctc
     6181 cgccgcagcc ccaggaacag aaggaaacgg atgaaccgcc acctgcgcct gatgtcactc
     6241 agccacctgc accaaatctt gctcctgcga acggtcgcca accaccggca gccactgatt
     6301 ctggtcgcat gatagtatca ccgctggcgg cgcgtatggc tgccgaggcg ggcatcgatc
     6361 ttcgttcact gcaagggagt ggaccaggcg ggcgcatcat caagcgagac atcgaagcag
     6421 tgatc
//
LOCUS       DASSFQ010000314         6398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1781800, whole genome shotgun sequence.
ACCESSION   DASSFQ010000314 DASSFQ010000000
VERSION     DASSFQ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781800"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFH96_11270"
     CDS             complement(<1..314)
                     /locus_tag="VFH96_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974599.1"
                     /translation="MRLGAWACVLLFVLTSHASAQIDRSDNQLWSDVQVAVPLTKNID
                     FNMLGTLRLGRDISRPVDERLGTGFTFRFGQHLQIAPNYLYIGMQPFRNRRVWESRLS
                     LPV"
     gene            complement(311..1552)
                     /locus_tag="VFH96_11275"
     CDS             complement(311..1552)
                     /locus_tag="VFH96_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974600.1"
                     /translation="MLTEISMAKAKETFIPRLNEPASSAPPSAAAASVVGISKRFGKA
                     SVLENISFDVGEGEVLVLLGASGSGKTTILRIIAGLEMPYTGKVILHGKDVTELPARE
                     RGVGVIFQSYALFPKMTVEKNIGYGLRIRKRKRKEIKETVNELLSLVQLEEHRKKYPS
                     QLSGGQQQRVAIARTLAYKPEVLLFDEPFGALDTQTRVHLRREIRALLKKVNVPSIFI
                     THDQEEALELGDRVAVLNVGHIEQIGTPFEIYNHPATEYVATFLGAANVLDAVVRQDF
                     IEVGSAQIPAPTLDRAKFKPGDCVKIVFRPEDVSLHRGDFVRSTHPRLANAIIEEIAF
                     VGAYERVRLQMETGPDECKTDETSFLLTTETPDRKPSKSIIATRPKPEASATRLHAGE
                     RVVVALTSFTVLPAQGPKGAS"
     gene            complement(1542..2378)
                     /locus_tag="VFH96_11280"
     CDS             complement(1542..2378)
                     /locus_tag="VFH96_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HET6974601.1"
                     /translation="MSSIAPAMRHVRGFDVARPLSIGMLVFMMLPLVFLPLASIFIFG
                     TSNGLDKFWGALTAPEAIFALKLSMATSFWATVFNVIFGLFAAYVLSRYNFWGRNALI
                     VTISLPTAIPTAVAGFALLLLWGPYGTLGRLLAPHGFQIMFTAFAIILANIFVTFPLA
                     FGVIKPVFDTMSHSLEEASATIGATRWQTFWHVTLPSLRGAIVSGALLTFARAVGEFG
                     STILVSGNLAGRTQTAPLYIFAKFNEGQIEAANAIAIVLALFSFLIFSVLFFARDWLD
                     VD"
     gene            complement(2466..3485)
                     /locus_tag="VFH96_11285"
     CDS             complement(2466..3485)
                     /locus_tag="VFH96_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6974602.1"
                     /translation="MRKRTSRGIPFLATAALVMALLAASCLPKPPGEKGDINITLYGF
                     SIMKESLEKAIFPGFTAKWKQQHGQTVQFQSSFAGSETVTNQILRGAPAEIAILSIER
                     DVQRLQKDGFVTADWHSLPGKGIVNKTPFVIIVRQGNPKGIHDFSDLAKPGIRLIHPD
                     PISSGGAQWSILAIYGSELKKSEDDKPKALQTLRAIWKNVISTPGSAREARTTFELGN
                     GDALITYELEALLMKEAKAPIEVVVPQSTIFSEHPACVVDKNITAAKRPVVDAFMEYL
                     WSDEAQQAFVKFHFYSSTNDVFNQANKEFGHIEMPFTIEYFGGWDKAYPEVIEGIFRD
                     QVQRK"
     gene            3639..5990
                     /locus_tag="VFH96_11290"
     CDS             3639..5990
                     /locus_tag="VFH96_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET6974603.1"
                     /translation="MLLRKAIALLLAQAFFLPIAFGQQQTPEATRPRRTQPVWTPPSA
                     TTPIISTPTLAPTTGPEPKIRVALNTDARSAVVSTTGHLMNASGAGTSLVALDTSRVR
                     VDPRLLSPLPAATADGGFRVLVGGAATREEAEENEKDIQKITGEDAHAAYDTETKTWG
                     MFVGGKLSREEAEEFRARLEKAGIDATIEGKETVAQNQSTTNNSPVRLTSRSALPSRE
                     VVASSGAGKMFSSSAPVSFASDDEQNSPVRYNERPYRGRIEVFTNLRGTLTVVNELGL
                     EDYVRGVIANELSPGGYPAIEAQKAQAIAARTYALRNRGQFMSQGFDILPTTRSQVYR
                     GLTSENPLSTRAVDETRGIIATYNGEPINALYTSTCGGRTEDSEKIFNEAIPYLRAHE
                     CAGESFDRFVIKTSREPADLQDDANVVLARDVSLLLTQNFGTLRERVSDAWLSADATA
                     SEVRNWLANVARVARQMAPASGDDVNRPPAFATALALAVFGESRAGTLLNDADADYLL
                     AVKDVQEVPAANRADLAMLLRDGHLSVYPDATLRPRTPLSRARVLHAISRLLEARNLL
                     QLQKANARPASDGQLILRSSKGKDQPIRVNPDAFLFRYLGERAFAVSSVAIVGGEPVT
                     YHLAPNGQIDFLEVRPATNGAAADRFSPFANWTTGLSVAQVQARLARAARGIGSITDL
                     RVASRGASHRVTDLEVVGTSGTAHVRGGRIRSALGLREQLFVIDREYDDNGRVSEFVF
                     TGRGWGHGVGMCQVGAYGLARQGWSAEQILKTYYSGIELTRMY"
     gene            6069..>6398
                     /locus_tag="VFH96_11295"
     CDS             6069..>6398
                     /locus_tag="VFH96_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HET6974604.1"
                     /translation="MNLTRNQLLAGLILLILLWLVILIRMFAIVGPTASGKSTLGIEV
                     ALEINGEIINCDSVQVYKEIEIATAKVPLDERKGVPHHLIDFVSPNINYTAVEWAREA
                     TKTIEEIE"
BASE COUNT         1412 a         1816 c         1799 g         1371 t
ORIGIN      
        1 accggcaacg atagtcgact ctcccacact cttcggttac gaaagggctg catgccgatg
       61 tagagatagt ttggcgcgat ctgcaggtgt tgtccgaacc tgaacgtgaa gcctgtgcca
      121 aggcgctcgt caaccggacg gctgatgtcg cgaccgagac gcaatgtccc gagcatgttg
      181 aagtcgatgt tcttcgttaa cggcaccgcg acttgaacat cgctccagag ctggttatca
      241 gatcgatcaa tctgcgctga cgcgtgactc gtcagcacaa acaacagcac gcacgcccac
      301 gcgcctaacc tcaacttgca ccctttgggc cttgcgccgg caagaccgtg aatgaagtta
      361 gcgcgaccac cacacgctcg ccggcatgca accgcgtagc actcgcttca ggcttcggac
      421 gcgtggcaat gatcgatttg ctgggctttc ggtccggcgt ttccgtggtc aggagaaaac
      481 tcgtctcgtc tgttttgcac tcgtccggtc cggtttccat ctgcagacgc acgcgttcat
      541 aagcaccgac gaaagcgatc tcttcgataa tcgcgttcgc gagtcgcggg tgagtagagc
      601 gcacgaaatc tccccgatgc aacgacacgt cttcaggtcg aaagacaatt ttcacgcaat
      661 cgcccggctt gaacttcgct cggtccaacg tcggcgcggg tatctgcgcc gaacccacct
      721 cgataaaatc ctgtcgtaca actgcgtcga gaacattcgc cgcgccgagg aacgtcgcca
      781 catactccgt ggccggatga ttgtagattt caaacggcgt tccgatctgt tcgatatggc
      841 ccacgttaag caccgcgaca cgatcgccga gttcgagcgc ttcttcctga tcgtgcgtga
      901 tgaagatcga gggcacgttc acctttttca ggagggcgcg tatttcacga cgcaggtgaa
      961 cgcgtgtctg cgtgtcgagt gctccgaacg gttcgtcgaa caataagact tccggtttgt
     1021 aagcaagtgt gcgtgcgatt gcgacgcgct gttgctggcc gcccgagagc tgcgaaggat
     1081 attttttgcg atgctcttcg agttggacga gggacaacaa ctcgttcacg gtttccttaa
     1141 tctcttttcg tttgcgcttg cggatacgaa gaccgtaacc gatgttcttc tccacggtca
     1201 tcttcggaaa gagcgcgtac gattgaaaga tcacgccgac gcctcgttct ctcgcaggca
     1261 gctcagtgac gtctttgccg tgcaggatca ctttgccggt gtacggcatt tccagaccgg
     1321 caatgatgcg caggatcgtc gtcttgccgc tgcctgaggc gccgagcaac acgagcactt
     1381 cgccttcacc gacatcgaag ctgatgtttt cgagcacgct cgccttgcca aagcgtttgc
     1441 tgatcccgac cacgctcgct gcagccgctg acggcggcgc gcttgacgcg ggctcgttta
     1501 gtctcgggat aaatgtttct ttcgctttcg ccatgctgat ctcagtcaac atccagccag
     1561 tcgcgcgcga agaacagcac gctgaaaatc agaaacgaaa acaacgcgag cacgatcgcg
     1621 atcgcatttg cggcttcgat ctggccctcg ttgaacttcg cgaagatgta caacggcgcc
     1681 gtctgtgttc gtcccgcaag gtttcctgac acgaggatcg tcgagccaaa ctcaccgaca
     1741 gcacgcgcga aagtcagcag cgcgccggag acaatcgcac cgcgcaaaga cggcagcgtg
     1801 acgtgccaga acgtttgcca gcgcgtggcg ccgatcgttg ctgaagcttc ttcaagcgag
     1861 tggctcatcg tgtcgaatac gggcttgatg acgccaaacg cgagcgggaa cgtcacgaat
     1921 atgttcgcga gaataattgc gaacgcagtg aacatgattt gaaaaccatg cggcgcgagc
     1981 agacgtccga gcgtgccgta cggtccccat agcagtaaca gtgcgaagcc cgcgacagcg
     2041 gttgggatcg cggtgggaag cgagatcgtg acgatgagcg cgttgcgtcc ccagaagttg
     2101 taacgcgata acacgtacgc tgcgaagaga ccgaagatga cgttaaagac ggtggcccag
     2161 aagctggtcg ccatcgagag cttgagtgca aatatcgctt cgggagcagt cagtgcgccc
     2221 cagaacttat cgagtccatt gctcgtgccg aagataaaga tcgaagcaag tggcaggaac
     2281 acgagcggca acatcataaa cacgagcatg ccgatactga gcggccgcgc cacatcaaac
     2341 ccacgcacat gcctcatcgc tggagctatg gaactcatat aaaaccgtaa cgcggatgac
     2401 acggatgaaa acgcggataa agacggatct tttaaatccg gtttttgatc cgtgttatcc
     2461 gtggctcatt tacgttgtac ctggtcccga aagattccct caatcacctc gggatacgct
     2521 ttgtcccaac caccaaaata ttcgatcgtg aacggcatct cgatatgccc aaactccttg
     2581 ttcgcctggt tgaacacgtc attcgtgctc gaataaaaat gaaacttaac gaacgcctgc
     2641 tgcgcttcat cactccacaa atactccata aacgcatcaa ccaccgggcg cttcgccgcc
     2701 gtgatgttct tatcgaccac gcacgccgga tgctcgctga agatcgtcga ctgcggcacc
     2761 accacttcga tcggcgcctt cgcttctttc atcaacaacg cttccagctc gtaagttatc
     2821 agcgcgtcgc cgttgccgag ctcgaaagtc gttcgtgctt cacgcgccga tcccggcgtt
     2881 gagatcacgt tcttccaaat cgcgcgcagt gtttggagcg ccttcggctt atcgtcctct
     2941 gacttcttca actccgatcc gtaaatcgcg agtatcgacc actgcgcgcc acccgaactg
     3001 atcggatcgg gatggatcag tcggattccc ggttttgcca gatcggaaaa atcgtgaatc
     3061 cccttcgggt tgccctggcg cacgatgatc acgaaaggcg tcttgttgac gattcccttt
     3121 ccgggtaaag aatgccagtc ggcagtcaca aaaccgtctt tttgcaatcg ctgcacgtca
     3181 cgctcgatgg agaggatcgc gatctctgcg ggagcgccgc gcaaaatctg gttggtcaca
     3241 gtctctgaac ccgcaaacga cgactgaaac tgcaccgtct gcccgtgttg ctgtttccat
     3301 tttgcggtga agcccggaaa gatcgccttc tcgagcgatt ctttcatgat cgaaaaaccg
     3361 tagagcgtga tgttgatgtc gcccttttcg ccggggggct ttggcagaca gctggcggcg
     3421 agcagcgcca tgacgagcgc ggcggtcgca agaaacggaa tacctcggga agtacgcttc
     3481 ctcatgcaat ccttaattct ggcctaaggg ggttgcacgt ctataccaca gagtcgagga
     3541 tacgatccac agattacgca gatttcacag aaggcaatta ggaaagttca ggaaaatctg
     3601 tgcaatctgt ggatggtttt tgtagtagta tgcgggcgat gctgctacgg aaagccattg
     3661 ctttgctgtt ggctcaggca ttttttctcc ccatcgcatt cggtcaacaa caaactcctg
     3721 aagccactcg accacgccgg acgcaaccgg tttggacgcc gccgtctgcc acgactccga
     3781 tcatttccac gccaacactc gcaccaacga ctggtccgga accgaagatc cgcgtcgcgc
     3841 tgaatactga tgcacgttct gctgtcgttt cgacaacggg ccatctgatg aacgcgagtg
     3901 gcgccggcac gagtttagtg gctttggata cgtcacgtgt tcgtgtcgat ccgagactgc
     3961 tgtctccgct tccagcggcg acagcggatg gcggatttcg agtgctcgtc ggcggcgcgg
     4021 caacgcgtga agaggcagaa gagaacgaga aagacattca gaagatcacg ggcgaagatg
     4081 cgcacgccgc ttatgacacc gaaactaaaa cgtggggcat gtttgtcggc ggcaagcttt
     4141 cccgggaaga agccgaagag tttcgcgcgc gtctcgaaaa agccggcatc gacgcgacga
     4201 ttgagggaaa agaaaccgtc gcgcaaaatc aatcgactac aaacaacagc ccggtgcgtc
     4261 ttacttcgcg atcggcgctg ccatcgcggg aagtcgtcgc ttcatccggg gcaggaaaga
     4321 tgttcagctc gagtgcacca gtctcgtttg catccgacga cgagcagaac tcgccagtgc
     4381 gttacaacga gcgaccatac cgcggacgaa tcgaagtgtt cacaaatctg cgcgggacgc
     4441 tcacagtcgt aaacgagctc ggacttgaag actacgttcg cggcgtcatt gctaatgagc
     4501 tttcgcctgg cggttacccg gcgatcgaag cgcaaaaagc acaggctatc gcggcgcgca
     4561 cctacgcgct caggaatcgc gggcaattca tgtcgcaagg gttcgatatc ctgccgacaa
     4621 cgcgctcgca agtttatcgt ggactcactt ctgagaatcc attatcgaca cgcgctgttg
     4681 acgaaacacg cggcattatc gcgacctaca acggcgagcc gatcaacgcg ctctacacgt
     4741 cgacctgcgg cggccgcacg gaagattccg aaaagatttt caacgaggcg attccctact
     4801 tgcgcgcgca tgaatgtgcg ggagagtcgt tcgaccggtt cgtgatcaaa acatcgcgcg
     4861 agccggcgga cttgcaggac gatgcgaatg tcgtgctcgc gcgtgacgtc agtctgttac
     4921 tgacgcaaaa cttcgggacc ttgcgagagc gtgtttcgga tgcgtggttg tcagccgatg
     4981 ccacagcgtc agaagtgcga aactggttgg cgaatgtcgc ccgcgtcgct cggcaaatgg
     5041 cgcccgcttc gggtgacgac gtgaatcgcc cgcctgcttt tgcgaccgcg ctcgcgctgg
     5101 ctgtgtttgg tgagagccgc gccgggacgc tgctgaatga cgctgatgcc gattacctgt
     5161 tggcggtgaa agatgtgcag gaggtgcccg cagctaatcg tgctgacctc gcgatgctgc
     5221 tgcgcgacgg gcatctttcg gtctatccgg acgcgacgct gagaccgcgc acaccgttgt
     5281 cgcgagcgcg cgtgctgcac gcgatctcgc gccttctcga agcgcggaac cttctgcaac
     5341 tgcaaaaagc gaacgcgcgt ccggccagtg atggacaact gattctgcgt tccagcaaag
     5401 gaaaggatca gccgatcaga gtaaatcctg atgcgtttct gtttcgctat ctcggtgagc
     5461 gcgcctttgc ggtgtcgtct gtagcgatcg tcggtggtga gccggtgacg tatcatctcg
     5521 cgccgaatgg acagatcgat tttctcgaag tgcggccggc gacaaacgga gcggcggcag
     5581 atcgattctc gccgtttgct aattggacca ccggtttgag tgtcgcgcag gtacaggcgc
     5641 gtttggcgcg tgcagcgcga ggcattggat cgattacaga cctgcgtgtg gcgtcgcgtg
     5701 gcgcgtcaca tcgagtgact gatctggagg ttgtcgggac cagcggtacg gcgcatgtgc
     5761 gtggtggtcg tattcgatcg gcgctgggcc tgcgcgaaca attattcgtg atcgaccgcg
     5821 agtatgatga caacggacgt gtcagcgagt ttgttttcac cgggcgcggt tggggtcacg
     5881 gcgtcggcat gtgtcaggtc ggcgcctacg gactcgcgcg gcagggctgg tccgctgagc
     5941 aaatactaaa aacttattat tccggtatcg agctcacgcg catgtattag tatccacaga
     6001 ttacgcagat ttcgcagact gtttaagaca tctgcgtaat ctgtgtaatc tgtggataaa
     6061 aaatcctcat gaacctcaca cgaaatcaac tactcgccgg cctcattctg ctcattctgc
     6121 tctggctggt tatactcatt cgcatgtttg cgatcgtcgg tccaaccgcg tcaggtaaaa
     6181 gcacgctcgg catcgaggtc gccctcgaaa tcaacggtga gatcatcaac tgtgattcgg
     6241 tccaggtcta caaagagata gaaatcgcta cggccaaagt ccctctcgac gagcgcaaag
     6301 gcgtcccaca tcacctgatc gacttcgtct ctccgaacat caactacact gccgtcgaat
     6361 gggcccgcga ggccacaaag acaatcgagg aaatcgaa
//
LOCUS       DASSFQ010000315         7543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1924304, whole genome shotgun sequence.
ACCESSION   DASSFQ010000315 DASSFQ010000000
VERSION     DASSFQ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924304"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFH96_11300"
     CDS             <1..289
                     /locus_tag="VFH96_11300"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6974605.1"
                     /translation="DQYPDITFQSTNVTAKMSAANRYDVKITGNLTLHGVTKRIMIPA
                     VVTLEGNNLRAVGEFKIDRDDFNVKATSAFHGMVRVDDDIRFEFDIVGRRS"
     gene            complement(361..1122)
                     /locus_tag="VFH96_11305"
     CDS             complement(361..1122)
                     /locus_tag="VFH96_11305"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET6974606.1"
                     /translation="MKAELQKLIALQNLDTTIRKLEKDQEAIPERRAEIEGEFDQRAF
                     EIRALETRRDEAKHNRARLENEVVEQRGRAERAERNLQASKKPDEYTAAIREADSARK
                     QISALETQILEQMEALDQAQAALTERADEIASLNSDREARLKAFDDETGTIGGRLEAA
                     RKEREEVFAALPKPMSSQYSRIKARIRDGVAVAEARNRSCSACFMSLRPQVMSEIRRG
                     EDIITCDNCGRILFYVPAEAVKTDAVTPQPNVAVS"
     gene            complement(1268..2341)
                     /locus_tag="VFH96_11310"
     CDS             complement(1268..2341)
                     /locus_tag="VFH96_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6974607.1"
                     /translation="MKVKYPIGLLLALILTAGVALAQSAPPAPPEPPDDVLDQNFSVF
                     IDGNGFLGVYAENISRENMGRYHVNQVRGVGITQIVKDSPAEKAGLRKDDVILRLDGE
                     NISSVRKLNRIVSELSPDQSVKVAISRGGSEQELTATIGKRSNTNMVQNLLGDGPRIF
                     KWEGPDFKNFKWETPLANKGQLFNQGDMTFFLNNSRRIGVSTMSLTKQLADYFGIADG
                     KGALVTSVSEDSPAANAGVKAGDVITAVDGEAVDSPGDISRVVNRKKEGDVTLTIIRN
                     KSQQTIRVTPREGGGFSGTLGNPQIGRRIVIPRIEIPTIPDIDIDVAMPNIVIPSIPT
                     INVQMPRIRVTPRIRTRGVQGPI"
     gene            complement(2419..3372)
                     /locus_tag="VFH96_11315"
     CDS             complement(2419..3372)
                     /locus_tag="VFH96_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6974608.1"
                     /translation="MRVVPISLPTPFYIGPVNVYLIAEDPLTIIDTGPKTKETIEALR
                     AGLRNAGFLVSDLRRIVLTHAHEDHCGLAKSLRDEAKNAEVFVHNWETGHSHSRLQYE
                     EHLTLLVRAGVPTAEIERMRALYEYMRRYSDSLGDDEHTSLWDEGELEFESGSLRILH
                     TPGHTPGSCSFLREADRTVIAGDCVLKRITPNPVLSPDPMDPSKRFPSLAEYMVSLAR
                     LRSLSPTLVYGGHGEPIRDFEELFHRYFRAFQERQSQVIGLVTKSGVTAWEVALKMFP
                     TADDVHRFLSVSEAVAHLDMAHSEGKIAYELSNQTDIYRSV"
     gene            complement(3373..5157)
                     /locus_tag="VFH96_11320"
     CDS             complement(3373..5157)
                     /locus_tag="VFH96_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6974609.1"
                     /translation="MSAVAEQKKLIKGGAFLIEERVPEEIFTPEDFTDEHRMIADTTR
                     QFVDNEVVTRIDELEKHDWKLSRELVGKAAELGLIGANIPEEFGGLGLDQTSGALIGE
                     NIGRSASFATTLGAESGIGLLPIVYFATDAAKQKYLPKIASGELITAYALTEAGSGSD
                     ALAAKATARLSDDGKEYILNGEKMWITNGGFADIFIVFAKVDGDKFSAFIVERQPGLT
                     SGAEEHKIGIKGSSTTALVLSDVRTPVENLLGEVGKGHKIAFNVLNIGRFKLGAMCVG
                     GMKLMVHESVRYANERHQFGKSISSFGAIKAKLAEQAIRTWIGESMIYRTLGMIEAGV
                     GAVDSKDMDAKLRAIEEYAAECSIIKVALSEYCDFVVDEMVQIYGGYGYSADYPAERA
                     YRDSRINRIFEGTNEINRMLIPGRLMKSALSGNLPLLPAAQALMDEVLSPQIGGFDDD
                     ETLLAAEQKLTTNAKKVALMTLGTAAQKYMMKLGDQQEILMGIADIIMDTYAMESAIL
                     RARKLAAAQGETAAARYLDMTRVFCNDAVGRIETRAKSTLAGMSEGDELRTLLAALRR
                     FTKLQPMNTIAARQRIADDMIAANKWIY"
     gene            complement(5172..6338)
                     /locus_tag="VFH96_11325"
     CDS             complement(5172..6338)
                     /locus_tag="VFH96_11325"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET6974610.1"
                     /translation="MIVSAVRTAVGKAPKGTLRSTRPDEMGGTVIKEALARVPGLEAS
                     EIEDVIMGCAMPEAEQGMNVARSAAIRAGLPVETSAMTINRFCSSGLQSIAMAADRIK
                     SGGADVIVAGGLETMSMIPMGGHIIRPNPYLVDHYPDFYLSMGLATENVARKYEVTRE
                     QQDEFAFRSHARAAAALDADKFKDEVVPLYVVLEELDEKGKKQRREVVFDKDEGVRRD
                     TSAEGLAKLRPAFHVKGTITAGNASQMSDGAAAAIVMSDSRARELGAKPMARFIAYAT
                     AGCPPEEMGIGPVFAIPKALKLAGLTLNDIDVIELNEAFAAQSIAVIKTLGLDPDKVN
                     VNGGAIALGHPLGCTGAKLTASILRELERRNGRYGMVTMCVGGGMGAAGILERI"
     gene            complement(6403..>7543)
                     /locus_tag="VFH96_11330"
     CDS             complement(6403..>7543)
                     /locus_tag="VFH96_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET6974611.1"
                     /translation="MTVPANVEQMLASGAKSFYKKEDAQQFYFDFASGKYVQLPDPPG
                     VLILKSIKDRTGVIKKNAGASLIDIGDGVACLEFHSKMNAIGGDTLQMLKYSLGEVEK
                     NFVGLVVGNQGVNFCVGANIMLMLMEAQEENWEELDMMGRVFQAATMSLRYSPKPVVV
                     APFQMVFGGGCEMVLHGDRVRAAAETYIGLVEVGVGIIPAGGGTKEMLLRVMDSIPKG
                     VDDADPFPFVKCAFETMALAKVATSAEEARSLGFLTKDDSISMNADRLIADAKKEVLA
                     LAASGYVQPQQRKDILALGDPALATLKLGIHQMKRAGYISDHDAEIGTQLARILTGGD
                     LNHPTRVSEQYLLDLEREAFLKLVGMRKTQERIAHMLKTGKPLRN"
BASE COUNT         1589 a         2265 c         1919 g         1770 t
ORIGIN      
        1 ggatcagtat ccggacatca cttttcagag cacgaatgtg acggcaaaga tgtccgcggc
       61 caaccgctac gatgtaaaga ttacggggaa cctaacgctg catggcgtca cgaagcgaat
      121 catgattccg gcagtcgtta cactcgaggg aaacaatctg cgggcggtgg gtgagttcaa
      181 aatcgatcgc gacgatttca acgtgaaagc gacgtctgcg tttcacggga tggtgagagt
      241 tgatgacgat atacggtttg agtttgatat cgtgggaaga agaagttaat tttgccgcgg
      301 attcacgcgg attaacgctg atctgcattc atctgcgttt tatccgcggc taaaaaactg
      361 ttagctcact gcaacgttcg gctgtggtgt tacagcgtca gtcttgaccg cttccgcagg
      421 cacataaaaa agaatgcgtc cgcagttatc acacgtgatg atgtcttcac cgcgacgaat
      481 ctcagacatc acctgtggtc gcagcgacat gaagcaagca ctgcatgacc gattgcgcgc
      541 ctctgcgact gccacaccat cacgaatgcg agctttgatt cgcgagtact gactgctcat
      601 cggtttcggc aacgcggcaa acacctcttc acgttcttta cgagccgcct ctaaccggcc
      661 tccgatcgtt cccgtttcgt cgtcgaaggc tttcaaacgc gcttcacgat ccgagttcag
      721 actggcaatc tcgtcggctc gctcagtcaa tgctgcttgt gcctggtcga gcgcctccat
      781 ttgttcgagg atttgtgttt ccagcgcgga gatttgtttg cgggctgaat cggcttcacg
      841 aatcgcagca gtgtattcgt ccggcttttt cgacgcctgc aagtttcgtt cagctctctc
      901 ggcccggccc ctttgttcga cgacctcgtt ttccagtcgc gcgcgattgt gtttggcttc
      961 gtcgcggcga gtttccagtg ctctaatttc aaaggcgcgc tgatcgaact ctccttcgat
     1021 ctcagcgcgc ctttcgggta tcgcttcctg gtctttttct agttttcgta tggtcgtatc
     1081 aagattctgt aaagcgatta acttctgcaa ttccgctttc acttatgcac tccttaaaag
     1141 atcaggacta aaaatgacgt gtttcatggt ttacggatca tcacataacc actgcgtctg
     1201 acgcaagtat ccacagatta cacagatgaa tctgcgtaat ctgtggattg ttctgctatc
     1261 gacctggtta gattgggcct tggacgccgc gagttcttat tctaggtgtc acccggatac
     1321 gtggcatctg gacgttgatt gtcggaattg aggggatgac gatgttgggc atggcgacgt
     1381 cgatgtcgat gtcgggaatc gtcggaatct cgatgcgcgg aatcacgatg cgacgtccga
     1441 tttgcgggtt acctagcgtt ccggagaaac caccaccttc gcgtggtgtt acgcgaatcg
     1501 tttgctgtga cttgttgcga atgatcgtga gtgtgacgtc gccctctttc ttgcgattga
     1561 ccacgcgcga gatgtcgccc ggagaatcga ctgcttcacc atcgaccgct gtaatcacgt
     1621 caccggcttt gacgcctgca ttcgccgctg gactatcttc gctgactgaa gtcacgagtg
     1681 cgcctttgcc gtcggcgata ccgaaataat cggcgagttg tttggtgagc gacatcgtgc
     1741 tcacgccgat gcgacggctg ttgttcagaa agaacgtcat gtcgccctga ttaaataact
     1801 ggcccttgtt agccaacggc gtttcccact tgaagttttt gaaatcggga ccttcccact
     1861 tgaatatgcg tggtccgtcg ccgagcaggt tctggaccat gtttgtgttg ctgcgtttgc
     1921 cgatggtcgc ggtcagttcc tgttcacttc cgccacgact gatcgcgact ttcactgact
     1981 ggtctggcga caactcagag acgatgcgat tgagcttacg aacactcgag atgttctcgc
     2041 cgtcgagccg caggataacg tcgtcctttc gtaaacctgc tttctcagcc ggactgtctt
     2101 tgacgatttg ggtgatgccg acgccacgca cctgattgac gtgatagcgg cccatgttct
     2161 cgcggctgat gttttcggcg tagacgccga ggaagccatt accgtcgata aagactgaaa
     2221 agttttggtc aagtacatcg tcaggcggct caggaggcgc gggcggtgca gattgcgcca
     2281 gcgcgactcc tgcggtcaga attagagcaa gcaggagacc gataggatat ttcactttca
     2341 ttttcgagtt tcctccggaa gagttttgcc attacacgca ctctccgcgt cgaggttccg
     2401 aaaaatatta gccacggatt acacggatcg gtaaatgtcc gtttgattgc tgagttcgta
     2461 ggcgattttg ccttcggaat gggccatgtc gaggtgggcg acggcttcgg agacggataa
     2521 aaaccggtgg acgtcgtcgg cggtgggaaa cattttgagc gcgacttccc aggcagttac
     2581 cccgcttttg gtgacgaggc caatgacctg tgactgccgc tcctgaaacg cccggaagta
     2641 acgatggaac aactcttcaa aatctcggat cggttctcca tgtccaccat agactaacgt
     2701 cggcgacaga gatcttaagc gcgccaaact gaccatgtac tcggcaagcg acggaaaacg
     2761 cttcgacgga tccatcgggt cgggcgacaa caccggattc ggcgtgatcc tcttcaacac
     2821 gcaatcgccg gcaatcaccg tacgatctgc ctcacgcaga aacgagcacg agccgggcgt
     2881 gtgtcccggc gtatgaagaa tgcgcaaact gccgctttca aactcgagtt ccccttcatc
     2941 ccaaagcgac gtgtgttcat cgtcgccaag cgagtcagaa tatctgcgca tgtactcgta
     3001 caacgcacgc atgcgctcga tctcggccgt aggcacaccc gcgcgcacca gcaacgttag
     3061 atgttcttcg tactggagac ggctgtgaga atgtcccgtc tcccagttgt gaacaaacac
     3121 ctccgcattc tttgcttcat cacgcaaaga tttcgcgagt ccacaatgat cttcgtgtgc
     3181 gtgagtgagc acgatgcggc gcagatcaga aacgaggaaa cccgcgttgc gcaaacccgc
     3241 gcgcaatgcc tcgatcgtct ccttcgtctt cggcccggta tcgatgatcg tcaacggatc
     3301 ttcggcgatc agatagacgt ttaccgggcc aatataaaaa ggcgtgggca gtgagatggg
     3361 aacgacgcgc acctaataga tccacttgtt agcagcgatc atgtcgtcgg caatacgctg
     3421 acgcgcggca atcgtgttca tcggctgcaa cttcgtgaac cgtcgcagcg cggccaataa
     3481 cgtgcgcagc tcatcgcctt ccgacattcc cgctagagtg ctcttcgcgc gtgtctcgat
     3541 tcggccgact gcgtcgttgc agaacacacg cgtcatgtcg agatagcgcg cagcggctgt
     3601 ttcaccctga gccgcagcga gtttgcgcgc gcgcaggatc gcggactcca tcgcgtacgt
     3661 gtccatgatg atgtcggcga tacccatcag aatctcctgt tgatcgccaa gcttcatcat
     3721 gtacttctgc gccgccgtgc cgagcgtcat caacgcgact ttcttcgcgt tcgttgtcag
     3781 cttctgttcc gcggcaagca atgtttcgtc gtcgtcaaaa ccgccgatct gcggcgacaa
     3841 gacctcatcc atcaaagcct gagcggccgg aagcagcggc agatttccgc tcaacgcgct
     3901 cttcatcagg cgaccgggaa tcagcatgcg attgatttcg ttggtgcctt cgaagatgcg
     3961 attgatacgc gagtcgcggt aagcacgctc agccggataa tctgcggagt aaccgtagcc
     4021 gccgtagatc tggaccatct cgtcgacgac gaaatcgcag tactcggaga gtgctacctt
     4081 aatgatcgaa cactcagcgg cgtactcttc gatcgcgcgc agctttgcat ccatgtcctt
     4141 cgagtcaacc gcaccgacgc ccgcctcaat cattccgagc gtgcgataaa tcatcgactc
     4201 gccaatccac gtgcgaatcg cctgttccgc gagtttcgcc ttaatcgcgc caaacgacga
     4261 gattgatttg ccaaactggt gccgctcatt cgcgtaacgc accgactcat ggaccatcaa
     4321 cttcatgccg ccgacgcaca ttgcgcccag cttgaagcgg ccaatgttca gcacgttgaa
     4381 agcgatcttg tgacctttgc cgacttcgcc gagcaggttc tcgaccggcg tccgcacatc
     4441 ggaaagcacg agcgcagtcg tactcgaacc cttgatgcca atcttgtgct cttcggcgcc
     4501 cgaagtcagt cctggctggc gctcgacgat gaacgcgctg aacttgtcgc cgtcgacttt
     4561 cgcaaagacg ataaagatgt cggcgaagcc gccgttggtg atccacatct tctcgccgtt
     4621 cagaatgtat tccttgccgt cgtcactcaa gcgcgcagta gctttcgccg ccagcgcgtc
     4681 tgaacccgaa ccggcttcgg tcagcgcgta agcagtgatc aactcgccgc tcgcaatctt
     4741 cggcaggtat ttttgcttcg cggcgtcggt agcgaagtaa acgattggca gcaacccgat
     4801 gccactctct gcgccgagcg tcgtggcaaa cgaagctgag cgtccgatgt tctcgccgat
     4861 aagggcgcca cttgtttggt cgagaccgag gccgccaaac tcttccggaa tgttggcgcc
     4921 gatcaagccg agttcagccg ctttgccgac gagttcgcgc gacagtttcc aatcgtgctt
     4981 ttcgagttca tcgatgcgcg tcaccacttc gttgtcgaca aactgccgcg tggtgtcggc
     5041 gatcatgcga tgttcgtcgg tgaagtcttc gggcgtaaag atctcttcgg gaacacgctc
     5101 ctcgatcagg aatgcgccac ctttaattag tttcttctgt tcggccacgg ctgacatcgg
     5161 tttctctcct tttagattct ttccagaatt cccgcggcgc ccatgccgcc gccaacacac
     5221 atcgtcacca ttccatatct tccattgcga cgttcgagtt cccgcaggat gctcgcagtc
     5281 aactttgcgc ccgtgcatcc gagcggatgg cccaacgcga tcgcaccgcc gttgacgttg
     5341 accttatcgg gatcgagacc caacgtcttg atcacagcga tcgactgcgc cgcaaacgct
     5401 tcgtttagtt cgattacatc aatatcgttg agcgtcagac cggcgagctt caacgccttc
     5461 ggaattgcga acactggccc aatacccatc tcctcgggcg gacaacccgc agtggcgtaa
     5521 gcaatgaaac gcgccattgg cttcgcgccg agttcgcgtg cgcgcgagtc agacatcacg
     5581 atcgccgccg cggcgccgtc agacatttgc gacgcgttgc cggccgtgat cgttcccttc
     5641 acgtgaaagg cgggcctgag tttcgccaaa ccttccgccg acgtgtcgcg tctgacacct
     5701 tcgtctttat caaacaccac ttcgcgtcgc tgcttcttac ctttttcgtc caactcttcc
     5761 aacacgacgt atagcggcac tacttcatcc ttaaacttgt ctgcatcgag cgccgccgcc
     5821 gctcgtgcat gcgagcgaaa tgcaaactca tcctgttgct cgcgcgtcac ttcatacttg
     5881 cgcgcgacgt tctcggttgc gagacccatg ctcaggtaga agtcaggata atgatcgacg
     5941 agatacggat tcggccggat gatgtgacca cccatcggga tcatcgacat cgtttccaaa
     6001 ccacccgcga cgatcacatc cgcgccaccg ctcttgatcc ggtcagccgc catcgcaatc
     6061 gactgcaagc ctgacgaaca aaaacgattg atagtcatcg cgctcgtctc aaccggcagg
     6121 cccgcgcgaa tcgcagcaga gcgagcgacg ttcatcccct gctccgcctc cggcatcgcg
     6181 caacccataa tcacgtcttc aatctccgac gcttcgagcc cgggcacacg cgccagtgct
     6241 tccttaataa cagtcccacc catctcatcc ggcctggtcg agcgcaaagt ccccttcggc
     6301 gccttcccga ccgccgtcct caccgctgat acaatcactg cctctctcat aattaacccc
     6361 aatccgtgta aatcgtttct tatgatccgt aaaatccgtg gcttaattcc gtagcggctt
     6421 cccagtcttc agcatgtgcg caatgcgctc ctgcgtcttg cgcattccaa ccaacttcaa
     6481 gaacgcctca cgttccaagt ccaacaaata ctgctcggaa acacgcgtcg gatgattcag
     6541 atcaccaccc gtcaggatgc gcgcgagctg cgtcccaatc tcagcatcgt gatccgaaat
     6601 ataccccgcc cgcttcatct gatgaatccc gagtttcaac gtcgcgagcg caggatctcc
     6661 caacgccaga atatctttgc gctgctgcgg ctgcacatag ccactcgccg ccagcgccaa
     6721 cacttccttc ttcgcatccg cgatcagacg atccgcattc atcgagatag aatcgtcctt
     6781 cgtcagaaat cccagcgacc tcgcttcttc cgctgaagtg gcaactttcg cgagggccat
     6841 cgtctcaaac gcgcacttca caaacggaaa cggatccgcg tcgtcaacac ccttcggaat
     6901 cgaatccatc acgcgcagca acatttcctt cgtgccgccg cccgcaggaa taattccgac
     6961 tccgacttca accaacccga tgtacgtctc cgccgctgct cggacgcgat cgccgtgtag
     7021 aaccatttca caaccgccgc caaacaccat ctgaaacggc gccacgacca ccggtttcgg
     7081 cgagtagcgc agactcatcg tggcagcctg aaagacgcgg cccatcatgt cgagctcttc
     7141 ccagttttct tcctgggctt ccatcaacat caacatgatg tttgcgccga cgcagaagtt
     7201 aacaccctga ttgccgacga caagcccgac aaagtttttc tcaacttcgc cgagcgagta
     7261 cttcaacatc tgcaacgtgt cgccgccaat cgcgttcatc ttcgagtgaa actcgagaca
     7321 cgccacgccg tcgccgatat cgatcagcga agcgccggca ttcttcttta ttactccggt
     7381 tctgtccttt atagacttga gaatcagaac gcctggagga tccggaagct gaacgtactt
     7441 tccgctcgcg aaatcgaagt aaaactgttg cgcgtcttct tttttgtaga acgacttcgc
     7501 accggaagcg agcatctgct cgacgttcgc cggcaccgtc atc
//
LOCUS       DASSFQ010000316        22823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_591387, whole genome shotgun sequence.
ACCESSION   DASSFQ010000316 DASSFQ010000000
VERSION     DASSFQ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591387"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFH96_11335"
     CDS             <1..1179
                     /locus_tag="VFH96_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974612.1"
                     /translation="GLPTIAVRDLNIQKRENDLVVGTFGRGIYILDNYTPLRSIKPEM
                     LRQEATVFPVKDALMYIQSQPLGGRGKSFQGERFYTADNPPYGATVTWYLKEAIKTKK
                     EKRQESERDAEKKGAPVGWPSGTELRAEEEEEAPSIIVTITDSSDNVVRRLTGPIGGG
                     IQRLTWDLRYPAANLSAPPPPDADFDFEPPSGPLVMPGTYKATVAKRVDGVVTQLGPT
                     QQFQVVVEGQENMSASDQTALVEFQTKAMRLQRAATGATLAANQLRPRLAAIKRAIAE
                     TPSLPQRLQDEATALDRRTNEIVRVLSGDAAARQRNINTPPSINDRIGYVVGAQRMSI
                     SRPTQTQIAQYNAAAQDFESVLGQLRQLFEVDLDKLEKQLEAAGAPWTPGRVPEWKNQ
                     "
     gene            1257..3734
                     /locus_tag="VFH96_11340"
     CDS             1257..3734
                     /locus_tag="VFH96_11340"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET6974613.1"
                     /translation="MKRFGILLLVLICFSTIHAQKISDKVAGMEKFPGFFPFYWDAKA
                     GKIWLEIDKWNTEFLYVESLPAGIGSNDIGLDRGQLGNSAIVRFDRTGPRVLLVAPNY
                     NFRAISNNADERLAVRDAFAESTLWGFEVAAEEGNRVLVDATRFYLRDVHQVTQTLQR
                     GQQGTYRLDESRSAFYLPNTKNFPQNTEVETTLTFTGEPTGQYVREVVPVPEAITVRE
                     RHSFVQLPPPGFKPRVFDPRAGYAGIDFMDFATPIEEPITKRLIARHRLQKKDPSARV
                     SEAVKPIVYYVDRGAPEPVRSALLEGARWWNQAFEAIGYKDAFRVEVMPAEVDPMDVR
                     YNVIQWVHRSSRGWSYGNSLTDPRTGEIIQGRVSLGSLRDRQDFLIAEGLLAPYDKTN
                     TASPKLLEMVLARLRQLSAHEVGHTLGLQHNYAASPVGRASVMDYPAPRAKLGPDGVP
                     DLSDAYAKGIGEWDKVAIAYGYQDFAPGTDENAALDRTLSEAFSRGLMYLTDQDARPP
                     SSASSVAHLWDNGTNAVDELGNVMKVRAAALRRFGENNIREGAPMATIEDVLVPIYMY
                     HRYQVEAAAKVIGGQDYTFSVKGKGDRNPQIVAPEEQRRALAAVLETIKPDALAVPET
                     LLRLIPPRPVGYQRTREDFRIRTQPVFDALAPAEAIADHVSGFLLNQERAARLVQFHA
                     RDPRNPGLTEVIDKILASTWKAPAATGYAGEIQHTVDMVILSDLMALAAGERASNQVR
                     AIASWKLDQLKTWLTAQSRFPVDENQRAFMFYAIVQIKRFQEDPKKANITPAQQPPDG
                     QPIGMDWWSRADGDCGHRWTQMPGDGN"
     gene            3771..5771
                     /locus_tag="VFH96_11345"
     CDS             3771..5771
                     /locus_tag="VFH96_11345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6974614.1"
                     /translation="MQGPEEYVGELLDEKYRLEHLLGKGGMGAVYLATHLGTERYVAL
                     KLISPQFMRNNEFVERFKREARAAGRLRHPNVVDVTDFGFARVKAGQVAYLVMEYLDG
                     CTLGDVLAEEKRLPLEWVADILEQVCSAVHEAHQQGIIHRDLKPDNIWLEPNRLGSYR
                     VKVLDFGIAKLGEAGAPPADDDPALTIIDDVAAAAALEHDTLMRPGGEEAGTQHFKAK
                     REAVSTVLQDPLTNPLLDHTDHADDDGTLLFDQKTNSGGRHTAHIATAPQGAALTRVG
                     AIMGTPLYMSPEQCGGGHVDTRSDIYSLGVIAYQMLAGEPPFAGNTSTVMRAHREQNP
                     QQLRERNNKIPKRAAGIVMSALSKNPGERPQTAFAFSSALRAQSEGVGALYRRAFALY
                     SEYFPKFIKLSLLAYLPVIITSILLLGVSLWYKYWPPVGTLHKVVVYSALGILGLLKF
                     VAGTISGGVISGVTAIIIVQLTAAPLRPVKLREAFVLLRKRWRPFLKTLIRVTLWILL
                     GIILLIIPGMVMWMRYSLYAPVVLIEGLEGKAAMRRARELASRSWKTIIVVAILQFLI
                     PAIVSACVGFMAGRAKHDSNTPSAQVFQQVLELANIFVIPLISIVPALLYVKMRQLGG
                     ESLASVWTQIEEGEGKQSAWQQRMRTRLSLHTPTTGQRTTGA"
     gene            5944..6561
                     /locus_tag="VFH96_11350"
     CDS             5944..6561
                     /locus_tag="VFH96_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HET6974615.1"
                     /translation="MLLFIDESGDPGLKIGAGSSKYFIVALVAFEDHDEALAADDRIA
                     LLRKEQGFPARFEYHFNKMKPSYRRMFLETVARYNFFYSGVVIDKARLTKSFKESIYK
                     YACGLLLEDVKPRLNNAIVVVDGSESMRFRQELKSYLVRRLKGDSGKCLVKDVRVHDS
                     TTNNLLQLADMVVGAVARSFSVKKDSREYRSLIDHREMNVEVWPK"
     gene            6997..8253
                     /locus_tag="VFH96_11355"
     CDS             6997..8253
                     /locus_tag="VFH96_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6974616.1"
                     /translation="MDIKRAPKSKIKKKIRNAIMIVVGIAAIGGITYGLTKLKPAAPT
                     LERSTAVIETVKRGEMVRDVRGNGTLVPEVTRWVPAPADGRVEKILLKAGVEVDPSTV
                     IAELSNPQLEQTASDTDLQVKAAQADQETLKVKLESDTMTQKAAIATINAQYSQAKLQ
                     LDADEVLAKQGLVADLTLKISRVNVQDLANRLKVEQERLTVNSKSTKAQLNAASSRLD
                     QLKALATLRRQQVDELKVRAGSGGVLQQVLVTEGQQVTPGTNIARVADPASLKAVLRV
                     AETQISGVRIGQPVIVDTRNGLIQGTVSRIDPAAREGTFEIDASLVGPLPPSARPDLS
                     VDGTIELERLKDVLKVGRPAFGQAQQTIGMFVLSPDGSEAERRSVKLGRNSVSQIEIL
                     EGLKEGDQVIISDTTALDSYNRIRVR"
     gene            8351..9109
                     /locus_tag="VFH96_11360"
     CDS             8351..9109
                     /locus_tag="VFH96_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974617.1"
                     /translation="MSSSEPLIHLADVKKVFYTDEVETHALSGIHLDIKQGEYVSIAG
                     PSGCGKSTLLSILGLLDTPTDGKYILNSHPVESLSLSERARIRNREVGFIFQSFNLIG
                     DLTVYENVELPLTYRGMSSGERKKRVTAALERVGMAHRAKHLPSQLSGGQQQRVAVAR
                     AVGGEPSILLADEPTGNLDSTNGEAVMELMRELHRAGATICMVTHDPRYAQHADRIVH
                     LFDGLIVEEEAGADRREIQEAKRELAESGFGAIQ"
     gene            9106..9798
                     /locus_tag="VFH96_11365"
     CDS             9106..9798
                     /locus_tag="VFH96_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6974618.1"
                     /translation="MISLRGVEKSFPHGPTRTYVLRRIDLDIRQGEFVSIMGPSGAGK
                     STLLHILGMHDQSWSGEYYFEDVPVHKLDGKKRAALRNQNIGFVFQSYHLLDDQTVFE
                     NLELPLSYRDVSKKDRQSIVCDTLDRFQMVGKKDLFPTQLSGGQQQLVGIARAVVASP
                     RIILADEPTGNLHSAQGKEIMDMFKRLNDEGTTIVQVTHSETNASYGNRIVQLEDGWI
                     VNPEQEQRTAVG"
     gene            9919..11085
                     /locus_tag="VFH96_11370"
     CDS             9919..11085
                     /locus_tag="VFH96_11370"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6974619.1"
                     /translation="MVNTVAAQVPRTLIADDQPDVLAALRLLLKGAGYQTEAANSPAA
                     VLDAIKHEKFDLVLMDLNYARDTTSGKEGLDLIERIHALDHDLPIIVMTAWATVDLAV
                     ESMRLGVRDFVQKPWENSRLLRKLKTQIDYGRRLRDREAAQQSRERQLKLELDEAREI
                     QRGLMPRRMPHLHGFSIANAWQPARGVSGDYLAAFKLDDARAALCVADVAGKGFPAAL
                     LMSNMQAALKSLASENSSPSELCGKLNLLMCGNTPLRKFITCFYSELDINTRKLTFTN
                     AGHNPPLLVRRDGECIMLEEGGRVIGAFCDSTFTQQEIQLNDGDKLLLFTDGVTEARN
                     AAGDEFGEQRLQACLRSYVGRNAAELRTLILDEVTAFCAGNFDDDATLMVLIAN"
     gene            11182..13590
                     /locus_tag="VFH96_11375"
     CDS             11182..13590
                     /locus_tag="VFH96_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974620.1"
                     /translation="MGDIRYALRNLLRRPTFTLIAVVTLALGIGANTAIFSAINALLL
                     KPLPFPELDRVVAIWDKLPSRGVMHNEVTVANYLDWQSQTQSFDQLALFRWWSTNLTG
                     IDPPERIQGFLVTANFLDVTGIKPIMGRNFSPEENQPGKDQVAIITHSLWQRRFGGDP
                     NILNKTITLNGVVRTVVGVMPERFNYPKGAEVYAPIQMTPELMKSRGSHSYYVIGRLK
                     SGSSIQSAQAEIDNIMARLEQQFPDTNKGWSSTVFPIVADTVRMYDTALWVMMAAVGF
                     VLLIACANVANLMLARASGRQREIAVRTALGASRWRIVRQLLTESLIVALIGGGLGIL
                     IGFLGVDALRAGNPGDAAKYAAGWDQLGINSTVLLFTLGLSVVSGIVFGLAPALQVSK
                     PNLNSSLKEGTRGTTSTTHRLRSALVVFEVALSLVLLVGAGLLVRSFLSLLKTDPGFN
                     PDNVLTMNLVLPRAKYKDEPAVAAFYSDLVQRVKAQPGVESAAFVNYVPLGGSNSSDS
                     YLVEGEPEPAPGQETDGRYRVATPDYFHTMDISIVRGRAFTEQDKAGTLPVVIVNETL
                     ARKHWPGQDPLGKRIRFYGPLDKAPWMQIVGVIKDVKHELNIPVEPEYYLPHAQDSWN
                     AMVLVARTTVDPASLSAALRQQVWAIDKDQPVFDVKTMQEVRSSSVAIYSFSSVMLAI
                     FAGIALVLAAIGIYGVMAFAVTQRTQEIGIRMALGARTADVLKLVVTHGMKLALLGIV
                     IGLAGSWALTRFIEKLLVGVQPTDLLTFSLVSVCLLFAAFVACYLPARRATKVDPLVA
                     LRYE"
     gene            13650..16064
                     /locus_tag="VFH96_11380"
     CDS             13650..16064
                     /locus_tag="VFH96_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974621.1"
                     /translation="MNSFFNDLRYALRGLVKRKGFAGIAVLTLALGIGATTATFTVVD
                     AVLLRKLPVADPDRVVVVHNQMPKINLPRTSVSAPHYVDYSRQTDVFESSAAFATRNF
                     NLTGVNIPERLQAGRVTATFFPTLGINPIAGRFFTPEEDKFGNERVVVLSTALWKRLY
                     SSNTGVLGNPIQLNGDNYQMIGVAPEGIEEIYPNVDFWVPMAFSARELSEERRGSLAY
                     TMLARLNHGTTLNHAQDVMTGVARNISNSDPDIFNIEIRSLTDEYVSDVRRPLFVLLC
                     AVIAVLLISCANVANLLLARATVRSHEIAVRVALGAGRSRIIQQLLTESLVIAVIGGA
                     FGILLAFWGTKALLALAPTTLPRVSSVQVDLRILLFSLGASLLCGVIFGLVPALTASK
                     TNLVSSLKESERTDSASATRQWLRRTFVVAEVAIALVLLISAGLLVRSFSKVLDVKPG
                     FDAQNVLTLRLSLPNAQYDKATKVAAFYDNVIARVSALPGVVHAGAAYQTPFTPGADN
                     SVFSIRGRQANPGDPPPHADYAFVSSDYFKTIGLPILKGRDFQPSDMRAGNYFAPNSV
                     AIVDEELAKRFWPNGDALGGGISWSNTGPYATVVGICATAQLKDLTEEAKGTFYLPSY
                     VGLSTLVVRTAGDPRPLTPAIREQVLAVDPNQPVYDVKTMDERVAVTLETRRFAVVLL
                     SVFGALALILAAIGLYGVLAFAVSQRTREIGIHMALGARARDVLLMVIRQGMSLVIVG
                     VMLGVAGAFALTRLMRSLLFEVGPTDLMTFVFVSLLLAVVGFIACYVPARRATKVDPL
                     IALRYE"
     gene            16103..18613
                     /locus_tag="VFH96_11385"
     CDS             16103..18613
                     /locus_tag="VFH96_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974622.1"
                     /translation="MENLLKDIRYGLRGLWKRKGFAAIAVLTLALGIGANTAIFTLVN
                     AVMLKSLPVERPEELVLFSDATGEGTSNEDTPRTGKWDLFSYASYDFLRNHNQSFVDI
                     TALRSGSSRLSVRRPEAAAAARGAGQLVSGNYFSVLGVRAARGRVLTNEDDKPGAAPA
                     AVISNRYWEQELNSDPSIVGKNFIINGTNFTIVGVTPREFFGERVRRPPDFWFPLSFQ
                     PQIELRESSLTNDQIYFLSVMGRLKPGTTIAQAQATTNLALRQFLTEQAGSKLTDERR
                     KGIENTYATLVEGQGGISGLRRAYSRPLQTLMVIVGMVLLIACANVGSLLLSRAASRK
                     AEISLRMALGATRWRIVRQLITESMLLALIGGVCGVLLAQWGVTVLVGLVAREAPLNT
                     RPDAGVLLFTAGVSIVAGLLFGLIPAVQASRTNLSSAMKEKNRLRSGFLRLSPSSLMV
                     VLQVGLSMVLLTGAGLFARSLLKLQSEDLGFNRNNVLLVGIDPRLAGYKTTELATLYQ
                     QVVERLGSLPQVQTVSIATYSPLSGTRRSSSVQVSGYTPKDGEDLATQDILVAPKYAE
                     TLGMPLLRGRDIDVRDTPTSTQVAVINSAFAEHFFKDQNPIGRTFSFDDDTDKGAAVQ
                     IVGVVGDIKRDDVRDKPDPTVYRPIFQIQEQGAYTATIHIRTQADPTPLTPQVRQLLT
                     QIDDKLPVFGVTTLSDQSRENLNQERLIAQLVSFFGALALILASIGLYGVMAQGVARR
                     TSEIGIRMALGARGGNIAWMILRETFYLVLAGLILGVPAALLGARLISTQLFGLSPTD
                     PLTIVGAAVVLSVVAMLAGYLPARRASRVNPLSALRYE"
     gene            18639..21044
                     /locus_tag="VFH96_11390"
     CDS             18639..21044
                     /locus_tag="VFH96_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974623.1"
                     /translation="MDSLIKDIRYGIRSLLKRPGFTAIALVALALGIGANTAIFSLVN
                     AVVLRPLPFPEPDQLVWGWGFVRNGPNRASVSPLDFVDYRNQSKTFENLAAASSIPLS
                     TNMTGNGDPERLTTYLVTGNYFDTIKIAPAIGRGFTLDNEKTGQDQVTVLSYDLWQRR
                     FGGDPGILNKSVVLNGKATEVVGVMGKDVTLPQSADLWLPINFDGDEMKMRKAHFLRP
                     VGRLKPGVTIRQAQADLDVVAAQLEKQYPDSNTGWSMRLVTLREQLLGGSRTYVFILF
                     GAVGLVLLIACANVANLLLVRAASRQKEIALRTALGASRLRILRQMITESVLLAILGG
                     ALGVLLARAGVELLVNLSGDNLPRTASVRVDGTVLLFTFLLALLTGLVFGLAPALRTL
                     QVNLIESLKEGVRGGGENTLKNRTRSVLVVAESAIAVVLLIGAGLLVRSLIAVQRVNP
                     GFDSNNVMTARLDIPRKKYDTPEKRAAFLTDIQTHLSNLPGVETVGLITELPLSGQPN
                     DMPFTVEGRPPLSPDQTLGADWRRVNENYFNALRIPLLRGRNFTAQEVRQSDPVVIVS
                     QQLVDQVFPNEDPLGKRLILAMGDKRYEIVGVIGDIRHRALAAPPVPTMYLPTFETGS
                     TNLAIRTKGDPTSITGSMRKEVIAVDSEQPIAAVRPMTALVETSVSSQRYNMMLLGLF
                     AVLAVVLAATGIYGVMSYTVAQRTHEIGVRMALGASRVDVLKIVLRQGMLLAAAGVFL
                     GLLGAYALTRVMAAFLFEVTARDPITFGMVSVLLLAVAFFACLVPALRATKVDPLVAL
                     RYE"
     gene            21076..>22823
                     /locus_tag="VFH96_11395"
     CDS             21076..>22823
                     /locus_tag="VFH96_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6974624.1"
                     /translation="MDSIIKDIRYGVRALLKHPGFTAIVVITLALGIGASTAIFSVVD
                     SVLLRRLPYRTAERIVAIQEVDPNGRRGQSTSANFYDWRQQQTVFEHLSAIKITTTNL
                     ALSDHAERIDLAQTNADFFDVFGITPQYGRLFIPQDEQAGHEPVVVMSNALWQRRFGS
                     DPSLVGRSITLDGKSYHVVGIAPPGFQYPDQTELWLPPLRLVPELYPDQDVTQTRRMG
                     YLFVVALLKPGVSLPQAAGEMETITARLRTQYPDSNNKRFNRVVSLHEHLVGDTNKLL
                     WLLLGAVTFVLLIGCANVANLLLVSGASRQKEIAIRVSLGASRWRVVRQLFTESMLLA
                     LTGGVVGLLIAFWGIAAITKLLPADFPRLNEIHIDLRVLGFTFVASMLTGILFGLVPA
                     LQVSRSDVQEAIRETGRGASGSRRQSRFRQALIVVEVALSVVLLAGAGLLFRSFLRLQ
                     SVETGFVSQEVLTARLTPSGTNYVNDADYAKFYNGVIEKVSALPGVQDAGLINTLPLF
                     KGPTSGFRVEGRPVTTPDKWPSVNYRVVSPNYFRAMGIPVLQGRTYTDRDNQNAPLVM
                     MVNQQLAREIFPDENPV"
BASE COUNT         4872 a         6385 c         6643 g         4923 t
ORIGIN      
        1 ggtctgccga cgatcgcggt ccgcgatctc aatattcaga agcgagaaaa cgacctcgtc
       61 gtcgggacct tcggtcgcgg tatttacatt cttgacaact acacaccgct gcgttcgatc
      121 aagcccgaaa tgctccgtca ggaagccacc gtgtttccgg ttaaggacgc gttgatgtac
      181 atccaatcgc agccactcgg cggtcgcggc aagagtttcc agggcgaacg cttctatacc
      241 gctgacaatc cgccgtacgg cgcgaccgtc acgtggtatc tgaaagaagc tatcaaaacg
      301 aagaaagaga aacggcagga gagcgagcgc gacgccgaga aaaaaggcgc gccggttggt
      361 tggcccagcg gcaccgagtt gcgtgctgaa gaagaggagg aagcgccgtc gatcatcgtc
      421 acgatcactg attcttctga caacgtggtg cggcgcttga ctggaccaat cggcggcgga
      481 atccagcgac taacctggga cctgcgttat cccgctgcca atctctccgc tccaccaccg
      541 cctgatgctg atttcgattt cgagccgccg tctggtccac tcgtgatgcc gggcacctat
      601 aaagccacag tcgcgaagcg tgttgatggt gtggtaacgc agcttggtcc aacgcagcag
      661 tttcaggttg tggtcgaggg ccaggaaaac atgtcggctt ccgatcaaac ggcgctcgtg
      721 gagtttcaga cgaaagcgat gcgtctgcaa cgtgcggcga cgggtgcgac tctggctgca
      781 aaccagctca gaccgcgact ggcggcaatc aaacgcgcga ttgcagaaac gccgtcactg
      841 ccgcaacgtt tgcaggatga ggcgaccgcg ttggatcgac gcacaaacga gattgtgcgc
      901 gtgttgagtg gtgacgctgc ggcgcgacaa cgcaacataa acacgccgcc gtcaatcaac
      961 gatcgcatcg gatacgtcgt cggcgcgcaa cgtatgtcca tctcgcgtcc gactcagact
     1021 cagatcgcgc aatacaacgc cgccgcgcag gatttcgaga gcgtgttggg ccagttacgg
     1081 cagttgttcg aagtcgatct ggacaaactc gagaaacaac tcgaagctgc gggcgcacca
     1141 tggactccag gccgcgtacc cgagtggaag aatcagtaaa cacggatttg gacggattaa
     1201 aaacacggat tcatccgtta gaaatccgtg ttatccgtgg ctaaaggaga tgtgacatga
     1261 agcgcttcgg gatcttgttg ctcgttctga tttgtttctc gacgatccat gcccaaaaga
     1321 tctccgataa ggtcgcgggc atggagaagt ttcccggctt cttcccattt tactgggacg
     1381 caaaagccgg aaagatctgg ctcgagatcg acaagtggaa caccgaattc ctctacgtcg
     1441 aatcgctgcc cgccggcatc gggtcaaacg acatcggtct cgatcggggt caactcggca
     1501 acagcgccat cgtccgtttt gatcgcactg gaccacgcgt gttgctggtc gcgccgaact
     1561 acaacttccg cgcgatcagt aacaacgcag acgagcgact cgcagtcaga gatgcgtttg
     1621 cggagtcgac gctatggggc ttcgaagtcg ccgctgaaga aggcaatcgc gtgctggtgg
     1681 acgcgacgag attttatttg cgcgacgttc accaggtgac gcaaacactg caacgcggac
     1741 agcagggaac ttatcgactc gatgagtcgc gcagcgcttt ctatcttccg aacacaaaaa
     1801 actttccgca aaacactgaa gtcgaaacca cgttgacgtt cacaggcgag cccacgggcc
     1861 aatacgttcg agaagtcgtg ccggtgcctg aagcgatcac ggttcgcgag cgtcattcct
     1921 ttgtgcaact gccgccgccg ggatttaagc cacgtgtgtt cgatccgcgc gccggttacg
     1981 ccggcattga cttcatggac ttcgcgacgc cgatcgagga gccgatcacg aagcgcctga
     2041 tcgcgcgaca tcgtttacaa aagaaggatc cgagtgcgcg tgtgagtgaa gccgtgaagc
     2101 cgatagtcta ttacgtcgat cgcggcgcgc ccgagccggt gcgctcggca ttgctcgaag
     2161 gcgcgcgctg gtggaatcag gcgtttgaag cgattggtta caaggatgca tttcgtgtcg
     2221 aggtcatgcc ggcagaagtg gatccgatgg atgtacgtta caacgtgatc cagtgggtgc
     2281 atcgctcgtc acgcggctgg tcctatggca actcactcac cgatccgcgc accggtgaga
     2341 tcattcaggg ccgcgtgtcg ctcggctcat tgcgcgatcg tcaggatttt ttgattgctg
     2401 aaggtctgct tgcaccgtac gacaaaacca acaccgcttc accgaaactt ctggagatgg
     2461 tgctggcgcg cttgcgacaa ctgtcggcgc acgaagtggg tcacacgctc ggactgcaac
     2521 acaactacgc cgcgagcccg gttggtcgag cgtcggtgat ggactatccc gcaccgcgcg
     2581 cgaagcttgg tccagacgga gttccggatc tgtctgatgc ctacgcgaaa ggcatcggtg
     2641 agtgggacaa ggtcgcgatt gcttacggtt accaggactt cgctcccggc actgacgaaa
     2701 acgccgcact cgatcgcacg ttgagcgagg ctttctcacg tggtttgatg tacctgaccg
     2761 atcaggacgc acgaccaccg agtagcgcga gctcggtggc ccatctctgg gacaacggta
     2821 cgaacgcagt cgacgagctc ggcaacgtga tgaaggtgcg tgccgcggcg ctgcgacgct
     2881 tcggcgagaa caacattcgt gaaggcgctc cgatggcgac gatcgaagac gtgctcgtgc
     2941 cgatctacat gtatcaccgt tatcaggttg aagctgcggc gaaagtgatt ggcgggcagg
     3001 actacacgtt cagcgtgaaa ggcaaaggcg atcgcaatcc gcagatcgtt gcgccggaag
     3061 aacagcgacg cgcgctcgcc gccgtgctcg agacgatcaa gccggatgcg ctggcagtgc
     3121 ccgagacgtt gttgcgtttg attccaccgc gcccggtggg ttaccagcgc acgcgtgaag
     3181 actttcgtat tcgcacgcag cccgtgtttg acgcgcttgc acccgcggag gcgattgccg
     3241 atcatgtcag tggatttctc ttgaatcagg aacgagcggc gcgcctggtc cagtttcacg
     3301 cgcgcgatcc tcgtaatccg ggtcttactg aagtgatcga caaaatactc gcctcgacgt
     3361 ggaaagcgcc cgctgctacg ggttatgcgg gcgagatcca gcatacagtc gacatggtga
     3421 tactttctga tctgatggcg cttgcggcag gcgagcgggc gtcgaatcag gtgcgcgcga
     3481 tcgcctcgtg gaagttggac cagctaaaga cgtggctgac ggcgcagagt cgttttccgg
     3541 ttgatgaaaa ccagcgtgcg tttatgtttt acgcgatcgt gcagataaaa cgttttcagg
     3601 aagatcctaa gaaggcgaat atcacgcctg cgcagcaacc acccgacggt caaccgatcg
     3661 gcatggactg gtggtcgcgc gccgacggcg attgcggcca cagatggaca caaatgcccg
     3721 gggatggaaa ctgatctaaa ttccaatccg tattacaatc cgtttttatt atgcagggtc
     3781 ccgaagaata cgttggtgag ttgctggacg agaagtatcg tctcgaacac ctgctcggaa
     3841 agggtggcat gggcgccgtc tacctcgcca cgcacctcgg caccgagcgt tacgtcgcgc
     3901 taaaactcat ctcgccgcag tttatgcgca acaacgaatt cgtcgagcgc ttcaaacgcg
     3961 aagcgcgcgc cgccggacgc ctgcgccatc cgaacgttgt tgacgtaact gacttcggtt
     4021 ttgcgcgtgt gaaagcgggg caggttgcat acctcgtgat ggagtatctc gacgggtgca
     4081 cgctcggcga tgtgcttgcg gaagagaagc gtctgccgct cgagtgggtc gccgacattc
     4141 tcgagcaggt ttgttccgca gttcacgaag cgcaccagca gggcatcatc catcgcgacc
     4201 tgaagcctga caacatctgg ctcgagccga atcgtctcgg tagttatcgc gtcaaggttc
     4261 tcgatttcgg tatcgcgaag ctcggcgagg caggtgcgcc gccggctgat gacgatccgg
     4321 cactcacgat catcgacgac gttgctgctg ccgcggcact cgagcacgat actctcatgc
     4381 gaccgggcgg cgaagaggcc ggcacacaac atttcaaagc gaaacgcgag gccgttagta
     4441 ccgttcttca ggatccgcta acgaatcctc tgctcgatca cacagatcat gcagatgatg
     4501 acggcacgct cctcttcgat cagaaaacaa atagtggagg acgacacact gcgcacatcg
     4561 cgactgctcc gcagggcgcg gcgttgacgc gcgtcggcgc gatcatgggt acgccgcttt
     4621 acatgtcacc cgagcaatgt ggtggtggtc acgtcgacac gcgttcagac atttatagtc
     4681 tcggcgtcat cgcttaccag atgcttgctg gtgaaccgcc ttttgccggt aatacttcga
     4741 cggtgatgcg tgcacatcgc gagcagaacc ctcaacagct tcgcgagcgc aacaacaaga
     4801 tcccgaagcg tgcggccggc attgtgatgt ccgcgctctc gaagaatccg ggggagcgcc
     4861 cgcagacagc gtttgcattt tcgagtgcgc tgcgagcaca aagcgaaggt gttggagcac
     4921 tgtatcgtcg cgcctttgcc ctttacagcg aatactttcc gaagtttata aagctgagcc
     4981 tgctcgcgta tctgccggtg atcatcacga gcattctgct gttaggtgtg tccctctggt
     5041 acaagtactg gccgccagta ggaacattac acaaggtggt ggtgtattca gcgctcggaa
     5101 ttctgggttt gttgaagttt gtggcaggca ctatttccgg cggtgttatt tcgggagtga
     5161 cggcgataat cattgtgcaa ctgaccgcag cgcctttgcg gcccgtgaag ctgcgcgagg
     5221 cgttcgtgct tttgcgcaag cgctggcgtc cctttctcaa aacgctgatc agggtgacct
     5281 tgtggatcct cctcgggata atcctgctga tcattccggg catggtgatg tggatgcgtt
     5341 attcactcta tgctccggtg gtgctcattg aaggactaga aggcaaagcc gccatgcgac
     5401 gcgcacgcga gctcgcgtca cgctcatgga aaacgattat cgtggtagcg atactccagt
     5461 ttctgatccc ggcgattgtc agtgcctgtg tcggctttat ggccggaaga gcgaagcatg
     5521 atagcaacac accttccgcg caggtgtttc agcaggttct tgaacttgca aacatcttcg
     5581 taatcccact gatctcgatc gtcccggctt tgctttacgt caagatgcgc cagctcggag
     5641 gtgagtcttt agcaagtgtc tggacgcaaa ttgaagaagg cgaaggaaaa caaagcgcct
     5701 ggcaacagcg catgcgcacc cgcctcagcc tgcacacccc caccacgggc cagcgtacca
     5761 ccggcgctta gctagttcat ttgcggtgtt tgtgcttctt tgggcacaat ctgcgagttc
     5821 tcacaaccca ctaaaaaacc ttggacctgc tcaagccgcg atctagttgc ggtgattgat
     5881 gcgatctcac tttgtcccct cacgagttag gggcaaagcg tcgatgcgtg ggagacctga
     5941 atcatgttgt tgtttatcga tgagtccggc gatcccggtt tgaagatcgg ggccggatcg
     6001 tccaaatact tcatcgttgc gcttgtagca tttgaggatc acgacgaggc gttagcggca
     6061 gatgatcgaa tcgcactgct gcgaaaagaa cagggatttc cagccagatt cgagtatcac
     6121 ttcaacaaga tgaagccttc ttatcgtcgc atgttcctcg aaactgtggc ccgttacaat
     6181 ttcttttatt ctggtgttgt cattgataag gcgaggctga cgaaaagttt taaagaatca
     6241 atttacaagt atgcctgtgg gctgcttctt gaagatgtaa agcctcgact gaacaacgca
     6301 atcgtggttg ttgacgggag tgagtcaatg aggttccgac aggagctaaa gagttatctc
     6361 gttcgtcggc tgaaaggtga ttctggcaaa tgccttgtca aggatgtcag agtacacgac
     6421 tccaccacaa acaaccttct gcaactggct gacatggttg tgggcgctgt ggcgaggtct
     6481 ttcagcgtaa agaaggattc tcgcgaatac cgaagcttaa ttgatcatcg cgaaatgaat
     6541 gtggaagtct ggccaaagta gtcgaccccg gccctatccc tttcgggaaa gccaccatac
     6601 ggtgaccttt cgggctcggg gccatttccg gcgccattct gtcttagagg ttgagggttg
     6661 tcaactattg cttggcttaa ccccatttcg aaagtgggat tcggatgtcc cgcaagcgaa
     6721 acgatcgttt acgttttttc gaacgtgcat ccgagtttgc gaccgcggcc tcattcccaa
     6781 agcgcccaga tttccttgtt tttagaacat ttccggcgca aactacgttc aactccgctg
     6841 ttggcacagg ctttgactcg caccgacgcg gagaactgta ctagcgatgc gtgaagtttg
     6901 tacctagctc gtttaattca ttcttcgcgc gcgcatcgcc gctctctcaa caatggctat
     6961 tacagctctc caaaattttc tttgaagtga agcactatgg atatcaagcg agcccctaaa
     7021 tccaagatta agaagaagat tcgcaacgcc atcatgatcg tggtcggaat cgccgcgatc
     7081 ggaggcatca cctacggcct caccaagttg aagccggctg cgccaactct cgagcgctcc
     7141 accgcagtta tcgagacagt caaacgcggc gagatggtcc gagacgtgcg cggcaacggc
     7201 acgctcgtgc ccgaggtcac gcgctgggtg ccggctccgg ctgacggtcg ggtcgagaaa
     7261 attcttctga aagcgggcgt cgaagttgat ccatccacgg tcatcgccga gctttccaat
     7321 cctcagctcg aacagacagc ttcggatact gacctgcagg tcaaagcagc tcaagctgat
     7381 caggagaccc tgaaggtgaa gctcgagagc gacacgatga cccaaaaggc cgcgattgcc
     7441 acgatcaacg cgcaatacag ccaggcaaag ttacaactcg acgccgacga agtactcgca
     7501 aagcagggat tggtggcaga tctcaccttg aagatatccc gcgtgaatgt tcaggatctt
     7561 gcgaaccggc tcaaagttga gcaggaacga ctcacagtca actcgaagtc gacgaaggcc
     7621 caattgaatg cggcctcttc acgcctggac cagttaaaag cgctcgccac actgcgacgt
     7681 cagcaagtgg atgaactgaa agtacgcgcc ggcagtggcg gcgttcttca gcaagtgctc
     7741 gtgactgaag gccagcaagt gacgcccgga acaaacatcg cacgcgttgc cgatcccgct
     7801 tcgttgaaag ccgttttgag agttgcggaa acgcagatct ccggcgtgcg catcggccag
     7861 ccagtcatcg ttgacacacg caacggtttg atccagggaa cagtctcgcg catcgacccg
     7921 gcagctcgcg aaggtacgtt tgagatcgat gcttcattgg ttggtccgtt gccgccaagt
     7981 gctcgtcctg acttgagcgt ggacggaacg atcgagctgg agcgactcaa agacgtcttg
     8041 aaagtcggtc gacccgcctt cggacaggca cagcaaacca tcggtatgtt cgtcctgagt
     8101 cccgacggat ctgaagcgga aagacgcagt gtgaaactcg ggcgcaactc cgtcagccag
     8161 atcgagatcc tcgaagggct gaaggaaggc gaccaggtca ttatttccga caccacagct
     8221 ttggacagtt acaaccgcat ccgggttagg taggccacaa aaaggcacaa aagactaatg
     8281 aggcacaaaa gcccggtccg cggtgttcag ttaaaaagat cgtgtacaaa aattcgttaa
     8341 ggagcgaatg atgagttctt ccgagcccct gattcacctt gctgatgtga agaaggtttt
     8401 ctacacagac gaggtcgaaa cacacgcact ctccggtatt catctcgata tcaaacaggg
     8461 tgaatacgtt tccatcgccg gaccttcggg ctgcggcaaa tccactctgc tctcaattct
     8521 tggcctgctg gatacgccga ctgacggtaa gtacatcctc aacagccatc cagtagagag
     8581 tctctcgctt tcagagcgcg cgagaatcag aaatcgcgaa gtgggtttca tcttccagag
     8641 cttcaacctg atcggcgacc tgacggtcta cgaaaacgtt gagctcccat tgacctatcg
     8701 cggcatgagc tccggcgagc gcaagaagcg agtcaccgcc gcactcgagc gcgtcggcat
     8761 ggcgcatcgc gcgaagcacc tgcccagcca gttgtccggc ggtcagcagc agcgcgtcgc
     8821 agtcgcacgt gcagttggcg gagagccgag catcctgctg gcggacgagc cgactggaaa
     8881 cctcgactcg accaacggcg aggccgtgat ggagctgatg cgtgagttgc accgggccgg
     8941 cgcgaccatc tgcatggtga cgcacgatcc acgctacgca cagcacgcag atcgcatagt
     9001 gcacttgttc gacggtttga ttgtcgaaga agaagccggc gccgatcgtc gtgagattca
     9061 ggaagcgaaa cgcgagctcg ccgagagcgg ctttggagca attcaatgat cagtttgcgc
     9121 ggcgttgaga agtcgtttcc gcacggtccg accaggactt acgtgctgcg aaggatcgat
     9181 ctggacatca ggcagggtga gtttgtgtcg atcatgggcc catcgggagc agggaagtcg
     9241 acactactgc acatcctcgg catgcatgac cagtcgtgga gcggtgagta ttacttcgaa
     9301 gacgtcccgg tccacaagct ggatggaaag aaacgcgccg cgctgcgcaa tcagaacatc
     9361 gggttcgttt ttcaaagcta tcacctgctg gacgatcaaa ccgttttcga gaacctcgaa
     9421 ctgccgcttt cttatcgcga tgtaagtaag aaggaccggc agtcgatcgt ttgcgacacg
     9481 ctcgatcgtt ttcagatggt gggcaagaaa gatctcttcc caactcagct gtcaggtggc
     9541 cagcagcagc tcgtaggcat tgcgcgcgcg gttgttgcga gtccgcgaat tatccttgcc
     9601 gacgagccga ctggaaatct tcattcggcc cagggcaagg aaatcatgga catgttcaag
     9661 cgtttgaatg atgagggcac cactatcgtc caggtaacgc actccgagac gaatgcctca
     9721 tatggcaatc gcatcgtgca gcttgaagac ggatggatcg ttaacccgga acaggagcag
     9781 cggaccgcgg tcggttaacg ttgcttcccc gaaccagttt ccctgtagtt gctcaagaag
     9841 ataatcacga acgtagaaat ctacgttgac gtcagaagtg tgtccgcagg tttagacacg
     9901 atttacagga ttaccgagat ggtgaatacc gtagcagcac aggttccccg tacgctcatc
     9961 gcagacgatc agccggatgt cctggccgcc ctgcggctgc tgctcaaagg cgccggttat
    10021 caaaccgaag cagcgaactc gcccgcggcc gttcttgatg cgatcaagca cgagaagttt
    10081 gatctcgtcc ttatggacct caactacgcg cgcgacacta cctcaggaaa agaaggtctg
    10141 gatctcatcg agcgaattca cgcgctcgat cacgaccttc cgatcatcgt gatgaccgca
    10201 tgggccacag tcgatctcgc ggtcgaatcg atgcgactgg gcgtgcgcga tttcgtgcag
    10261 aagccctggg aaaactcgcg cctgctacga aaattaaaaa ctcagatcga ttacggccgg
    10321 cgcctgcgcg atcgcgaagc ggcgcaacag agtcgtgagc gccagctgaa actcgagctc
    10381 gacgaagcgc gcgagattca acgcgggttg atgccgcgcc ggatgccgca cctgcacggt
    10441 ttcagtatcg cgaacgcgtg gcagcctgcg cgcggagtga gtggcgacta tcttgccgct
    10501 ttcaaattgg acgacgcgcg cgcggcgctg tgtgtggctg atgtcgcggg caaaggattt
    10561 ccggcagcgt tgttgatgtc gaacatgcag gcagcgttaa aatcgctggc gtcggaaaac
    10621 agctcgccga gtgagctttg cgggaagttg aacctgctca tgtgcgggaa caccccgctg
    10681 cgcaaattca tcacttgctt ttacagcgag ctcgatatca acacgcgcaa gctgacgttc
    10741 acgaatgcgg gacacaatcc gccactgctg gtccgccgcg atggtgaatg catcatgctt
    10801 gaagaaggcg gccgcgtaat cggcgctttc tgcgattcaa cttttaccca acaagagatt
    10861 caacttaacg acggcgacaa attgttgctg ttcacggatg gtgtcaccga agcgcgcaat
    10921 gccgcgggcg atgagtttgg cgagcagcgt ttgcaagcct gcctgcgatc gtatgtcggg
    10981 cgcaacgcgg ctgagttgcg gacgctgatc ctcgacgaag tcacagcgtt ctgcgccggc
    11041 aacttcgacg acgacgccac cctgatggtt ctgatcgcga actaggcaag aactctagat
    11101 ccatccacag attgcacaga tttttttctg tgtgatctgt gaaatctgtg gatgaaaaaa
    11161 ctgtggccca aaaggaggct catgggcgat attcgttacg cactgagaaa tctcttgagg
    11221 cgaccgacct ttacgctcat cgccgtcgtc accctcgcgc tcggcatcgg ggccaatacc
    11281 gctatcttca gcgccatcaa cgccctcctg ctaaaaccgc taccgttccc cgaactcgat
    11341 cgcgtcgttg caatctggga caagttgccc agtcgcggcg tcatgcacaa tgaagtcaca
    11401 gtcgcaaact acctcgactg gcaatcgcag acccaatcgt tcgatcaact cgcccttttt
    11461 cgttggtgga gcacaaatct caccggcatc gatccgcccg agcgcatcca gggattcctc
    11521 gtcacggcaa actttctcga cgtgaccggc ataaagccga tcatgggccg taacttctcg
    11581 cccgaagaaa accaaccagg caaggaccag gtcgcgatca tcacgcacag tctgtggcag
    11641 cgacgcttcg gcggcgatcc gaacatcctc aacaaaacga tcacgctcaa cggcgtcgtg
    11701 cgcacggtcg tgggcgtgat gccggaacgt ttcaactatc cgaaaggcgc tgaagtttac
    11761 gcgccaatac agatgacgcc ggagctgatg aagagccgcg gcagtcatag ttattacgtg
    11821 atcggcagat tgaaatcggg ctcgtcgatc cagagcgcac aggctgagat cgacaacatc
    11881 atggcgcgac tcgaacagca gtttcccgac accaacaaag gttggagctc aactgttttc
    11941 ccgatcgtcg ccgacaccgt gcgcatgtat gacacggcgc tgtgggtaat gatggccgcg
    12001 gttggattcg tgttgctgat cgcgtgcgcg aacgtcgcga acctcatgct cgcgcgtgcc
    12061 agtggccggc aaagggagat tgcagtgcga actgcgttgg gcgcgagtcg ctggcgcatc
    12121 gttcgccagt tgctgaccga gagcctgatc gttgcgttga taggcggcgg actgggaatt
    12181 ctgatcggct tcttgggcgt tgacgcgttg cgcgccggta atcccggcga cgcggcaaag
    12241 tacgcggccg gctgggacca gctcggcatc aattcgacag tgctattgtt cacgctcggg
    12301 ctttcggttg tgagcggcat cgtgtttggt ctcgctcctg ctttgcaggt atcgaaaccc
    12361 aacctcaaca gctcgctgaa agaaggaacg cgcggaacga ccagtacgac gcatcgacta
    12421 cgcagcgcgc tcgttgtttt tgaagtcgcg ctctcgctcg tgttgctcgt cggcgccgga
    12481 ctgcttgttc gcagctttct atcactgctc aaaactgatc ccggtttcaa tcctgacaac
    12541 gtgctcacga tgaatctcgt gttgcccagg gcgaagtaca aagacgagcc ggcggtcgcg
    12601 gcgttctaca gcgatctggt ccaaagagta aaagcgcaac ccggcgttga gtcagccgcg
    12661 ttcgtcaact acgtgccgct cggtggctcg aactcgtccg attcatacct cgttgaaggc
    12721 gagccggagc cggcgcctgg tcaggagacc gacggtcgtt atcgcgtcgc gacgcctgac
    12781 tacttccaca cgatggacat ctcgatcgtt cgtggtcgcg cgtttaccga gcaggacaaa
    12841 gccggcacgc tgccggtcgt catcgtcaac gaaactctcg cccgcaagca ctggcccggt
    12901 caggatccac tcggcaaacg cattcgcttc tatggaccac tcgataaagc gccgtggatg
    12961 cagatcgtgg gcgtcattaa agacgtgaag cacgagctga atattccggt ggagccggag
    13021 tattacctgc cgcacgcgca ggactcgtgg aacgccatgg tgctcgtagc gcgaacgact
    13081 gtcgatccag cgtcgctgtc ggctgcactg cgacaacagg tgtgggccat cgacaaggac
    13141 cagccggtgt ttgatgtgaa gacgatgcag gaagtgcgtt cgtcgtcggt tgcgatctac
    13201 tcgttctcgt cggtaatgct cgcgatcttc gcgggcatcg cgctcgtgct ggccgcaatc
    13261 gggatctacg gcgtgatggc gtttgcagtg acgcagcgca cgcaggagat cgggatccgc
    13321 atggcgctcg gcgcccgcac ggccgacgtg ctcaagctcg tagtgacgca cggaatgaag
    13381 ctggcgctgc tcggaatcgt gatcggcttg gccggatcgt gggcgctcac gcgattcatc
    13441 gagaagctgc tggtcggtgt gcaaccgacg gatctgctga cgtttagttt ggtttcggtt
    13501 tgtttactgt tcgccgcgtt tgttgcgtgc tatttgcctg cccgacgcgc aacgaaagtc
    13561 gatccgctgg tggcgctgag atatgaataa aaggcaatcc acagattaca cagaaaaaat
    13621 aatctgtgaa atctgtggat ggtttgatca tgaattcttt cttcaatgat ctgcgatatg
    13681 cactgagagg gttggtgaag cggaagggct ttgctgggat cgctgtgctc acgctcgccc
    13741 tcgggattgg tgccacgacc gcgacattta cggtggttga tgcagtgctg ctgcgcaaac
    13801 taccggttgc agatcccgat cgtgtggttg tggtccacaa ccagatgccg aagatcaatc
    13861 tgccgcgcac ttcggtctcg gctcctcact atgtcgacta cagtcgtcag accgatgtgt
    13921 tcgagtcgag cgcagccttt gcgacccgaa atttcaatct caccggcgtg aatatcccgg
    13981 agcgtttgca ggctggacgt gtgaccgcaa cgttcttccc gacgctcggg atcaatccga
    14041 tcgcaggccg gttcttcact cccgaagaag acaagtttgg taacgagcgc gtcgtggtgt
    14101 tgagcacggc tttgtggaag cggctctaca gctccaacac gggggttctc ggtaacccga
    14161 ttcaactcaa tggcgataac tatcaaatga ttggcgtcgc gcccgaaggt atcgaagaga
    14221 tctatccgaa cgttgatttc tgggtgccaa tggctttctc cgcgcgtgag ctttccgaag
    14281 agcgtcgcgg cagcctcgcc tacacgatgc tcgcgcgttt aaaccatggg accacgctca
    14341 accacgcgca ggacgtgatg acgggcgtcg cgcgcaatat aagcaactcg gatcctgaca
    14401 tcttcaacat cgaaatccgc tcactaacag acgaatatgt gagcgacgtg cggcggcccc
    14461 tctttgtttt gctgtgcgcg gtcatagcag tgttgttgat ttcgtgcgcg aacgtcgcca
    14521 atcttctgct ggcgcgtgcg actgtgcgca gtcacgagat cgcggtgcgc gtggccctcg
    14581 gcgccggccg tagtcgcatc attcaacaac tactcaccga gagtctcgtg atcgcagtta
    14641 tcggcggtgc tttcggaatc ctgctcgcgt tttggggaac gaaggctttg ctcgcgcttg
    14701 cacccaccac tttgccgcgt gtcagcagtg tccaggttga tctcaggatt cttttgttca
    14761 gtcttggcgc ttcacttctt tgtggcgtta tcttcggtct cgttccggct ttaacggcgt
    14821 cgaaaacgaa tctcgtcagc tcgctgaaag aatccgaacg aaccgactcg gccagcgcga
    14881 cgcggcaatg gttgcgcaga acattcgtcg tggcggaagt cgcgatcgcg ctcgtgttgt
    14941 tgatttcggc aggcttgctc gtccggagtt ttagcaaggt actcgacgtg aagccgggat
    15001 tcgacgcgca gaacgtgctc accttgcgcc tgtctttgcc gaatgctcaa tacgacaaag
    15061 ctacgaaagt cgctgccttc tacgacaacg tcatagcgcg tgtatcagca ttgccgggcg
    15121 tggtccatgc gggtgctgcg taccagactc cattcacgcc gggcgctgac aattcggtct
    15181 tctcgatacg cggcagacag gcgaatccag gcgacccgcc gccgcacgcg gattacgcat
    15241 ttgtatcttc cgattacttc aagaccatcg gcctgccgat tttgaaaggc cgcgattttc
    15301 aaccgtcgga catgcgcgcc ggcaattatt tcgcgccaaa ctcagtcgcc atcgtcgatg
    15361 aagaactggc gaaacgattc tggcccaacg gcgacgccct cggcggcggc attagctgga
    15421 gcaacaccgg accgtacgcg accgtcgtcg gcatctgcgc gaccgcacag ctcaaagatc
    15481 tcactgaaga agcgaaaggc actttctatc ttccgtcgta tgtcgggctt tcgacactcg
    15541 tggttcgcac tgccggagat ccacgcccgc taaccccggc gatacgcgaa caggtgctcg
    15601 cagtcgatcc gaatcaaccc gtttacgacg tgaagacgat ggacgagcgc gttgccgtga
    15661 cgctcgagac gcggcgcttt gcagtcgtgc tgttgagcgt gtttggagca ctcgcgctga
    15721 tactcgccgc gatcggttta tacggcgtgc tcgcattcgc ggtgtcgcaa cgcacccgcg
    15781 agatcggaat tcacatggcg ctcggcgccc gtgcgcgcga tgtgttgctg atggtgatca
    15841 ggcagggaat gtcgctcgtg atcgttggcg tgatgttggg cgtcgcgggt gcgtttgcgt
    15901 tgacgcggct aatgcgaagt ttgttgtttg aagttgggcc aacggatctg atgacgtttg
    15961 tgttcgtgtc gctgctgctg gcggtggtgg gctttattgc gtgctacgtg cctgcacgaa
    16021 gagcaacgaa ggtcgaccct ttgatcgcgc tgcgatacga gtaagaacag gccacggata
    16081 acacggataa agagcacgga ttatggagaa cttacttaaa gacattcggt atggtttacg
    16141 agggttgtgg aagcgcaaag gctttgccgc tatagccgtc ctcacgctcg cgctcggcat
    16201 cggcgctaac accgccatct tcacgctcgt gaatgcagta atgctgaagt cgcttccggt
    16261 tgaaaggccg gaggagttgg tcttgttcag tgacgcgacc ggcgagggaa cctcgaacga
    16321 agacacgcca cgcaccggta aatgggacct gttctcctat gcgtcctatg acttcctccg
    16381 caatcacaat caatcgttcg tggacatcac cgctctccgc agcgggtcga gccgcctgag
    16441 cgtgcggcgg cctgaggctg cagcagcggc gcgtggagcc ggacaactcg tctcgggcaa
    16501 ctatttttca gtactcggtg ttcgcgccgc gcgtggtcga gtgctcacca acgaagacga
    16561 caaacccggc gctgccccag ccgctgtgat cagtaatcgc tactgggagc aggaactaaa
    16621 cagcgatcca tcaatcgtcg gtaagaattt catcatcaac ggcacgaact tcaccatcgt
    16681 cggcgtcact ccaagagaat ttttcggcga gcgcgtgcgg cgtccgccag acttctggtt
    16741 cccgctgtcg tttcaaccac aaatagaatt gcgcgaatcg tccctgacca acgatcagat
    16801 ttacttcctg tcggtgatgg gccgtctgaa gcctggcacg accatcgcgc aagcgcaggc
    16861 gacgactaat cttgcattgc gacagttctt aaccgagcaa gccggcagca agctcactga
    16921 cgagcgacgc aaaggcatcg agaacactta cgcgacactc gtcgagggac agggcggtat
    16981 ctctggtttg cgtagagcgt attccaggcc actccagacg ctgatggtaa tcgtcggcat
    17041 ggttttattg atcgcctgcg ccaacgttgg cagtctcctg ttatcgcgcg cggcctcacg
    17101 caaggccgag atctctctgc ggatggccct cggcgcaact cgctggcgga tcgttcggca
    17161 actgattacc gaaagtatgt tgctggcact gatcggcggc gtgtgcggcg tgctattggc
    17221 ccaatggggc gtgacggttc tggttgggct cgttgccaga gaagcgccgc tcaatacgcg
    17281 gccagacgcc ggcgtgcttt tattcaccgc cggcgtgtcg attgtcgccg gtcttttgtt
    17341 cggactgatt cccgccgtgc aggccagtcg tacaaatctt tcctccgcga tgaaagaaaa
    17401 gaaccgcctg cgcagcggat tcctgcgact cagcccctcg tcactaatgg tagtgctgca
    17461 ggtcggtctt tcgatggtcc tactcaccgg cgcgggactg tttgcccgca gtctcctgaa
    17521 actgcagagc gaggacttgg gattcaatcg caacaacgta ctgctggttg gcatcgatcc
    17581 acgtctcgcc gggtataaga caacggagct ggccaccctt taccagcaag tcgtcgagcg
    17641 actgggttca ctgccgcagg tgcaaaccgt atcgatcgca acctactcgc cactgagcgg
    17701 cacgcgccgc agcagcagcg tccaggttag cggttatacg ccgaaggacg gcgaagatct
    17761 cgcgacgcag gacattttag tcgcgccaaa gtatgccgaa acactcggca tgccgttgtt
    17821 gcgaggacgt gacatcgacg tgcgcgacac gcccacgtcg acgcaagttg ccgtgatcaa
    17881 ttcggcgttc gccgagcatt tcttcaaaga ccaaaaccca atcgggcgca ccttctcatt
    17941 tgatgatgac actgacaaag gtgcagcggt gcaaatcgtt ggtgtggtcg gagacatcaa
    18001 acgcgacgac gtacgtgata agcctgaccc gaccgtctac cgtccgattt ttcagataca
    18061 ggagcagggc gcttacaccg ccacgattca cattcgcact caggccgatc ccactccgct
    18121 cacaccgcaa gtgcgacaac tgctcactca gatcgacgac aagcttcctg tcttcggggt
    18181 gaccacgctc agcgatcaat cgcgcgaaaa cctgaaccag gaacgtttga tcgcccagct
    18241 tgtcagtttc ttcggtgcgc ttgctttgat actcgcgagt attggtttgt acggcgtgat
    18301 ggcccagggg gtcgcgcgtc gcacgagcga gatcggaata cgcatggcgc tcggcgcgcg
    18361 tggcggcaac atcgcgtgga tgattttgcg agaaacattt tatctcgttt tagccggact
    18421 aattctgggt gtcccagctg cgttgctcgg ggcacgtttg atttcaacgc aactgtttgg
    18481 attgagcccg accgatcctc taacaatcgt gggagcggct gtcgttctgt cggtggtggc
    18541 gatgctggca ggttatctgc ctgcgcgacg tgcatcacgc gtcaacccgc tgagcgcgct
    18601 gagatatgaa tagaagcccc taaaaggagg gcagaaagat ggactcacta atcaaagata
    18661 ttcgctatgg gattcgcagc ttgttgaagc ggcccggatt caccgcgatc gccctcgtcg
    18721 cgctcgcgct tggcatcggc gccaacactg caatcttcag tctggtcaat gcagtcgtac
    18781 tgcgtccact tccgtttccg gagccggatc aactggtctg gggctgggga ttcgtgcgca
    18841 atggaccaaa ccgcgcgagc gtttctccac tcgacttcgt tgattatcgc aatcagagca
    18901 aaacgttcga aaacctcgct gccgccagct cgatccccct ttcgaccaac atgacgggca
    18961 acggcgatcc cgaacgactc actacctacc tcgtcaccgg caactatttc gacaccatca
    19021 aaatcgcccc ggcgatcgga cgcggcttca cgctcgataa cgagaagacg ggccaggatc
    19081 aggtcacggt gctcagttac gacctctggc agcgacgttt tggcggcgat cccggaattc
    19141 tcaataagtc ggtagtcctc aatggcaaag cgacggaagt cgtcggcgta atgggcaaag
    19201 acgttaccct gccgcagagc gccgaccttt ggctgcccat taattttgac ggcgacgaga
    19261 tgaaaatgcg taaggcgcat tttctgcgac cggtgggacg actcaagccc ggcgtcacga
    19321 tcaggcaagc gcaggccgat ctggatgtgg tcgccgcgca acttgaaaaa cagtatcccg
    19381 acagcaacac cggttggagc atgcgactcg tcaccttgcg agaacagctt ttgggtggaa
    19441 gcaggacgta tgtgttcatc ctgttcggcg cggtcggcct cgtgttgctg atcgcatgtg
    19501 cgaacgtagc caacctgctg ctcgtgcgtg cggcgtcgcg tcagaaagag atcgcgttaa
    19561 ggaccgcact cggtgcgagc cgtctgcgca tcctgcgcca gatgatcacg gagagtgtgt
    19621 tgctggcgat cctcggcggc gccctcggtg tattgctggc cagagccgga gttgagctgc
    19681 tcgttaattt gagcggcgac aatcttccac gcactgcgtc ggtccgagtt gatgggacag
    19741 tactactgtt cacttttctg ctcgcgcttt tgactggtct cgtgtttggc ctcgcgcctg
    19801 cgcttcgcac tctgcaagtg aatctgatcg agtcgttgaa ggaaggcgtg agaggtggcg
    19861 gcgaaaacac gctcaagaat cgcacgcgta gtgtgctcgt cgttgccgaa agcgcgattg
    19921 cagtcgtgtt gttaatcgga gcaggactgt tggttcgtag tctcatcgca gtgcaacgtg
    19981 tgaatcccgg cttcgattcg aacaacgtaa tgacggcgcg tttggacatt ccgcgcaaaa
    20041 agtacgacac gccggaaaag agagcagcct tccttaccga tattcagacg cacctcagta
    20101 acctgccggg cgtcgaaact gtcggactaa tcactgagct accgctgagc ggtcagccta
    20161 acgatatgcc gttcacggtc gaaggccgtc ctcctctttc gcctgaccaa acgctgggcg
    20221 ccgactggcg tcgcgtgaac gaaaactatt tcaacgcgct gcgcattccg ttattacgcg
    20281 ggcgaaattt taccgcgcag gaggtgcgac aaagcgaccc ggtcgtgatt gtcagccagc
    20341 aactcgtgga tcaggttttc ccgaatgagg atccactggg taaacgtttg attctggcga
    20401 tgggcgataa gcgctacgag atcgtcggcg tcatcggcga catccggcat cgcgcgttgg
    20461 cagcgccgcc ggttcccacg atgtatctcc cgacttttga gacgggttct acgaaccttg
    20521 cgattcgcac taaaggcgat ccgacgagca tcacgggcag catgcgcaaa gaagtgattg
    20581 cggtcgactc ggagcagccg atcgcggcag tcaggccgat gacggcgttg gttgagacgt
    20641 ccgtttcgtc gcaacgttac aacatgatgt tgctgggtct gtttgcagtg ctcgcagtgg
    20701 tgctcgcggc gacgggaatc tacggagtga tgtcttacac ggtggcgcag cgaactcacg
    20761 agatcggtgt gcgcatggcg ctgggcgcga gccgggtaga cgtgctgaag atcgtgctcc
    20821 ggcaagggat gttgctcgcg gctgccggcg tgtttttggg actgctcggc gcgtatgcgt
    20881 tgacgcgagt gatggcggcg ttcctgtttg aagtgacggc gagagatccg atcacttttg
    20941 ggatggtgtc agtgttgttg ttagcggtcg ctttctttgc gtgtctcgtt ccggcgctgc
    21001 gcgctacgaa ggtcgatccg ctggtggctc tgagatacga ataggaccaa tacgccctat
    21061 aggacctata aagtcatgga ctcaatcatc aaagacattc gctatggcgt gcgggcgttg
    21121 ttgaagcatc cgggattcac ggccattgtc gtgatcacgc tcgcgctcgg tatcggcgcg
    21181 agcaccgcga tcttcagcgt tgtcgatagc gtgctgttgc gtcgtttgcc ttatcgaacg
    21241 gcagaacgga tcgtcgcgat tcaggaagtt gatccgaacg gcaggcgagg ccagtcaacc
    21301 tcagctaact tctacgactg gcgccagcaa caaaccgtct ttgaacacct gtccgcgatt
    21361 aagatcacca cgaccaacct cgccctctcc gatcacgcgg agcggatcga tctcgcgcaa
    21421 acgaatgccg atttcttcga cgtcttcggc atcacgccgc aatacggacg gctgtttatt
    21481 ccgcaggacg agcaggcggg acacgagccg gttgtcgtca tgagcaatgc gctctggcag
    21541 cgtcgcttcg gatccgatcc gtctctcgtt ggcagatcca tcacgctcga cggcaaaagc
    21601 taccacgtcg tcggtatcgc gccgcccggt tttcaatatc ccgaccaaac cgaattgtgg
    21661 ctgccgccgt tgcgactcgt gccggagttg tacccggacc aggacgtgac ccaaacgcga
    21721 cgcatgggtt atctctttgt cgtcgcgttg ttgaaacccg gtgtgtcgct gccgcaggcc
    21781 gcaggcgaga tggagacgat cactgcgcgt ctgcgaaccc agtatcccga ttcaaacaac
    21841 aaacgtttca atcgagtggt ttcacttcac gaacacctcg tcggcgatac caacaaactg
    21901 ctctggctgt tgcttggcgc ggttacgttt gttttgctga tcggatgtgc gaacgtcgcg
    21961 aatctattgc tcgtcagcgg cgcatcgcga caaaaggaga tagcgattcg cgtatcgctc
    22021 ggcgcgtcgc gctggcgagt cgtacgccag ttgtttactg agagcatgct cctcgcgctt
    22081 accggtggcg tcgtcggatt gctgatcgcg ttttggggaa tcgcggcgat cacgaaactc
    22141 ctgccggctg actttccacg cttgaacgag attcacatcg acctgcgcgt gcttggcttc
    22201 accttcgttg cgtcaatgct caccggaata cttttcggcc tcgtgcccgc gctgcaagtc
    22261 tcgcgttccg acgttcagga agcgatcagg gaaaccggac gaggcgcatc aggaagccgg
    22321 cggcagagtc gttttcgaca ggcgctcatc gtcgtcgagg tggcgttgtc ggtggtgttg
    22381 ctcgccggcg ctggtttgct tttcagaagt tttctgcggc ttcagtcagt agaaacggga
    22441 tttgtttcgc aagaggtttt gactgcaagg ctgacgcctt caggcacaaa ctacgttaac
    22501 gacgctgatt acgcgaagtt ttataacggc gtgatcgaga aggtcagcgc gcttcccggc
    22561 gtgcaggatg ccggtctcat caacacgctg cctttattta aaggtccgac gtcgggtttc
    22621 cgcgttgaag gacgcccggt caccacgcct gacaaatggc cgagtgtgaa ctatcgcgtc
    22681 gtcagtccga actactttcg cgcgatgggt attccggtgt tgcaaggtcg cacttacacc
    22741 gatcgcgaca accaaaacgc gccgctcgtg atgatggtca atcagcaact cgcgcgcgag
    22801 atatttcctg atgaaaaccc ggt
//
LOCUS       DASSFQ010000317         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1474216, whole genome shotgun sequence.
ACCESSION   DASSFQ010000317 DASSFQ010000000
VERSION     DASSFQ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474216"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1934
                     /locus_tag="VFH96_11400"
     CDS             261..1934
                     /locus_tag="VFH96_11400"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6974625.1"
                     /translation="MAKPVLLTVDDDREVLRAIERDLRRKYGSDYRVLRAGSGQEALE
                     IVRELKIRNNSIALFLIDQRMPGMSGVEFLAQAIALFPDAKRVLLTAYADTDAAIAAI
                     NEAGINHYLLKPWDPPEENLYPVLDELLEDWEAAFPPQFEGIRVLGNRWSPASHNIKD
                     FLARNHVPYQWLDIETAASDSEVRSLVELLDEDETRSLPIVILPNGVRLRQPSLADVA
                     EHAGLKTRATEAFYDLAIVGGGPAGLAAAVYGASEGLRTVMIEREAPGGQAGMSSRIE
                     NYLGFPSGLTGADLARRAVVQARRFGVEIISAEATEVRVDGPYRFLKLADGAELSCHA
                     LLVATGVQWRKLDAPGVERLTGAGIYYGAAMTEAMSTKGEDVYVVGGANSAGQAAMHF
                     SRYARRVVMLVRGSSLAASMSQYLINQLKATPNIQIEYNTSVIEAHGEDHLEAISIHC
                     AETNEINKVPATGLFIMIGAAPNTDWLGEIVERDERGFIYSGPHLTRDGKRPNGWKLD
                     RDPGLLETNVPGIFVVGDVRHGSVKRVASGVGEGSVAISFVHQYLSKVV"
     gene            1938..>2316
                     /locus_tag="VFH96_11405"
     CDS             1938..>2316
                     /locus_tag="VFH96_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6974626.1"
                     /translation="MEETVSGGVEAIVPTTSSPADKVEALRRVHVFADLSDDQLRWFA
                     DNSEDRRFAAGDIMFRKGDKAEAMVVYLEGEVHAYWDESDHDIVYIGRAGEQTTEVSG
                     MLPFSRMKEFQVTGRAVTDVRLLW"
BASE COUNT          496 a          613 c          699 g          508 t
ORIGIN      
        1 gcttcgcagg ctaagaaatt agccaccgat tcaatgtgca tatagttttg tccaagccac
       61 actttaccgc aacttgacga agtttttctt cgtgctcgta atgccgcctc cggatcctcg
      121 tcactaagca gttgcacacg atcatccgcc gatacctcga tagtgttacc ccgcctatcc
      181 tcgatgctgt gatgcgccgg tgggcgttac gtggcagaat agtgctttgt tttttgcgca
      241 ttaatggagc actaatacgt atggcgaaac cggtgttact gacggtggac gacgatcgtg
      301 aggtgttgcg ggcgatcgag cgtgatctgc gacggaagta tggcagcgat tatcgtgtgt
      361 tacgggcggg atcgggacaa gaggctctcg aaatcgttcg cgagctgaag atcagaaaca
      421 actcgatcgc actctttctg atagatcaaa ggatgcccgg catgtcgggt gtcgagtttc
      481 tcgcacaagc gatcgcgctc tttccggatg cgaagcgtgt attgctaaca gcctacgccg
      541 acactgacgc agcaatcgca gcgattaacg aagcgggcat caatcattac ctgctcaagc
      601 cctgggatcc gccggaagaa aatctctatc cggtgctcga cgagctgctc gaggattggg
      661 aagcggcgtt cccaccgcag tttgaaggca tccgcgtcct gggcaatcgc tggtcgcccg
      721 cgtcgcataa catcaaagac tttctggccc gcaatcacgt tccgtatcaa tggctcgaca
      781 tcgaaactgc cgcttcggat tcagaagtcc gcagtctggt cgagttgctc gacgaggacg
      841 agacgcgcag cttaccgatc gtgattcttc ccaacggtgt gcgtttgcga caaccgtcgc
      901 tcgccgatgt ggccgaacat gccggactca aaactcgcgc gacagaagcg ttctacgatc
      961 tcgcgatcgt cggcggtggt ccagccggac tcgccgcggc tgtctacggc gcgagcgaag
     1021 gtttgcggac agttatgatc gagcgtgaag cgccgggtgg gcaggccggc atgtcgagtc
     1081 gtattgaaaa ctatcttggc tttccgagtg gactcacggg cgccgatctc gcgcgtcgag
     1141 ctgtggtcca ggcgagacgt ttcggggtcg agatcatctc agcggaagcc actgaagtgc
     1201 gagtcgatgg tccatacagg tttttaaaac tcgcagacgg cgcggagctg agttgtcatg
     1261 ccttgctggt tgcgactggc gtccagtggc gcaagctgga cgcgcccgga gtcgaacgtc
     1321 taaccggagc cggcatttat tacggcgccg cgatgaccga agcgatgtca acgaagggcg
     1381 aggacgttta cgttgtcggc ggcgcgaaca gtgcgggaca agccgcgatg cacttttcga
     1441 gatacgcgcg gcgcgtcgtt atgctcgtgc gcggttcgtc gctggccgcg tcaatgtcgc
     1501 aatatctgat caatcagctc aaagcaacgc caaacattca gatcgaatac aacacctctg
     1561 tcatagaggc gcatggcgaa gatcacctcg aagcgatttc aattcattgc gctgagacga
     1621 acgagataaa caaagttccc gccaccgggc tgttcatcat gatcggagca gcgccaaaca
     1681 ctgactggct tggcgagatc gtagaacgcg atgagcgcgg tttcatttat tccggcccgc
     1741 atctcacccg cgatggcaag cggcccaacg gttggaaact cgatcgcgat ccgggactgt
     1801 tagaaacaaa tgtgcccggc atcttcgttg tcggcgatgt tcgacacggc tcggtgaagc
     1861 gtgtggcctc gggcgtcggc gagggttcgg tggcgatctc tttcgtgcat caatatctaa
     1921 gcaaggtcgt gtgacagatg gaagaaacag tttcgggcgg cgtggaagca atcgttccaa
     1981 caacttcttc gcccgcagat aaagtcgaag cactgcgtcg cgttcacgtc tttgctgatc
     2041 tgtctgacga ccagttgcga tggttcgccg ataactctga agaccgtcgc tttgcggcgg
     2101 gagacatcat gtttcgcaaa ggcgataagg ccgaagcgat ggtggtctat ctggaaggcg
     2161 aggttcacgc ttactgggac gagagcgatc acgacattgt ttacatcggg cgtgcgggcg
     2221 agcagacaac cgaggtgagc gggatgcttc cgttttcacg gatgaaggag tttcaggtaa
     2281 ctggacgcgc cgtgacggat gtgcgcctgc tgtggt
//
LOCUS       DASSFQ010000318         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_592772, whole genome shotgun sequence.
ACCESSION   DASSFQ010000318 DASSFQ010000000
VERSION     DASSFQ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592772"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFH96_11410"
     CDS             complement(<1..420)
                     /locus_tag="VFH96_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974627.1"
                     /translation="MVYLVAAAQADDLARVREEKPELWRLVRRYNTPVQLALAAADEV
                     MRSSADPSTAAVISLAPCQPGSADLYRWGNVVTSGMASGTLGNLRMNPTQTLHAVDNL
                     AMSAFAIMHGNHAYCLGLGGAAGQAWCGLEAVYERLTA"
     gene            complement(432..1754)
                     /locus_tag="VFH96_11415"
     CDS             complement(432..1754)
                     /locus_tag="VFH96_11415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6974628.1"
                     /translation="MKIDIIHPAHYRDDGSLVQSKNLIDRLGAYLPHLGPPLMAALTP
                     EKHQVRLVEEYLEDIDFESDADVVALSGQIMQLRRCVDISKRFRAAGKKVVLGGYLPS
                     MLPDRVEGLFDAIVIGEGDELWPQLLDDIEQGKLKSRYRSSRPPDINNLPVPRYDLIK
                     KDRVVVYPVQATRGCPFTCEYCSIAAVYNGMYRKRPVDQIVRDVQATRSRNINFCDDN
                     LCEDVKFADRLFTALAGAPIRWGTQTTINVARHPELLKKARASGCVLMALGVETFSAR
                     NLEDVNKTFHAVDKYADGFKRIMDAGISPHALIIFGLPDDNPETFKRTVDYLESLKVP
                     ITQFFILTPYPGSPTGDKIWRSGVVFDEDLNHLREPYVVYKPQQLSPAELHDGWWRAV
                     QEFYSLRSIMRRVVLRRKPLNLWVNLATNLYYWSKVKRGIHTVYFGKN"
     gene            complement(1771..1971)
                     /locus_tag="VFH96_11420"
     CDS             complement(1771..1971)
                     /locus_tag="VFH96_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974629.1"
                     /translation="MPVRDCSPDEPLDYVIPFSSLILLGVVVAIEDRYGVRVTREQLG
                     RACESGTTLRRLADMIENLATD"
     gene            complement(2040..>2406)
                     /locus_tag="VFH96_11425"
     CDS             complement(2040..>2406)
                     /locus_tag="VFH96_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974630.1"
                     /translation="ATKVGEDSELILASLKSFLKDTFNSRNPLSAADIERRTEARSKA
                     LFIHNFMDPYDLDVARLKKCCTHYVMPDGRLMPGCSYNNLYRHKDERFFPDAEPVTPA
                     PVKLENGPALVQITGRVTQ"
BASE COUNT          553 a          733 c          624 g          496 t
ORIGIN      
        1 cgcggtcaga cgttcgtaca ccgcttcgag tccgcaccat gcctggcccg ccgcgccgcc
       61 aagcccgagg caatacgcgt gattaccatg catgatcgca aacgccgaca tcgcaagatt
      121 gtcgaccgca tgcagcgtct gcgtcggatt catgcgcagg ttgccgaggg ttccactcgc
      181 catgcctgac gtcaccacgt ttccccagcg ataaagatct gccgagcccg gctgacaggg
      241 cgcgagcgag atcacggccg cggttgacgg atctgcggaa gacctcatca cctcatcagc
      301 cgcagcgagc gcgagttgga ccggcgtgtt gtatcgacgc acgaggcgcc acaactccgg
      361 cttctcttct ctcaccctcg cgagatcgtc cgcctgcgcc gctgcaacca ggtaaaccat
      421 tttgtgcctt gctagttttt tccgaagtaa accgtgtgaa taccgcgctt cactttggac
      481 cagtaataca agttcgtcgc gagattaacc cacagattca acggctttcg tcgcaacaca
      541 acgcgcctca tgatcgaacg cagcgaataa aactcctgca ccgcacgcca ccaaccatcg
      601 tgcaactccg cgggcgaaag ctgctgcggc ttataaacaa catacggctc gcgcaagtga
      661 ttcagatcct catcaaacac cactccagag cgccagatct tgtctcccgt cggcgaaccc
      721 ggatagggcg tcaaaataaa aaactgcgtg atcgggacct tcaacgattc gaggtaatcg
      781 accgtgcgct tgaaggtttc ggggttgtcg tcaggcagtc cgaagatgat gagcgcatgc
      841 gggctgatcc ccgcgtccat gatgcgctta aaaccatcgg catacttatc aaccgcatgg
      901 aaagtcttgt tgacgtcttc aagattccgc gctgaaaaag tctcgacgcc gagcgccatc
      961 aacacacaac ccgatgcgcg cgcctttttc aaaagctccg gatgacgcgc aacattgatg
     1021 gtcgtttgcg tgccccaacg aatcggcgcg cctgcgagcg cagtgaagag tcgatcggca
     1081 aacttgacgt cttcgcacag gttgtcgtcg cagaagttga tattccggct acgcgtcgcc
     1141 tgcacgtcac gaacgatctg atcgacgggt cgcttgcgat acatgccgtt gtaaactgcc
     1201 gcgatcgagc agtactcaca agtgaaggga cagccgcgcg tggcctgtac cggatacacg
     1261 acgacccgat ccttcttgat gagatcgtag cgaggcacag gcagattgtt gatatcgggc
     1321 gggcgcgacg atcgatagcg actcttcagt tttccctgtt cgatatcgtc gagtaattgc
     1381 ggccaaagtt catcaccttc accgatgacg atcgcatcga aaagtccttc aactcgatcg
     1441 ggcaacatcg aaggcagata accacctagc acaaccttct tccccgccgc gcgaaaccgc
     1501 ttcgaaatat caacgcaacg ccgaagctgc atgatctggc ccgagagcgc gacgacatcg
     1561 gcatcactct cgaaatcgat atcttctaaa tattcttcaa ccaacctgac ctgatgcttc
     1621 tccggcgtga gcgcagccat cagcggtggt ccaagatgag gcaaatacgc accgagacga
     1681 tcaatcaggt tcttcgactg aaccagactg ccatcgtccc ggtagtgcgc tggatggata
     1741 atatcgatct tcatccgtct aaaatccgtg tcaatccgtg gctaaattct cgatcatgtc
     1801 cgctaaccgt ctgagcgttg tgccactttc acacgcacgc ccgagctgct cgcgcgtaac
     1861 acgaaccccg taacgatctt caatcgccac caccacgccc agcaatatca acgacgaaaa
     1921 agggatgaca tagtcgagcg gctcgtccgg cgagcaatca cgtacaggca tcgctgcatt
     1981 cacgtccggc gcggcacgaa cgatcagttc tcgcagctcg tcgattgtct cgggcctggc
     2041 tattgagtga ctctcccggt gatctggacc agtgccggtc cgttttcaag cttgactggc
     2101 gcaggcgtga ccggctcggc gtcggggaaa aaacgctcgt ccttatggcg atacagattg
     2161 ttgtacgaac agccaggcat gagtcgcccg tcgggcatca cgtaatgtgt gcaacacttc
     2221 ttcaagcgcg cgacatcgag gtcgtacgga tccatgaagt tgtgaatgaa gagcgccttg
     2281 ctccgcgcct ccgtgcgtcg ctcgatgtct gccgccgaaa gcggattgcg cgaattgaaa
     2341 gtgtctttga gaaacgactt cagcgacgcg agaatcaact ccgagtcttc accgactttc
     2401 gtcgcc
//
LOCUS       DASSFQ010000319        22897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1786559, whole genome shotgun sequence.
ACCESSION   DASSFQ010000319 DASSFQ010000000
VERSION     DASSFQ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22897
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786559"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFH96_11430"
     CDS             complement(<1..299)
                     /locus_tag="VFH96_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974631.1"
                     /translation="MSSCALTVISLLFLLPLRVVAQEPPRPPATPSPSPTATASPSPT
                     PEASPTPRDPMSTPTFNGLRMRSIGPAFTSGRVVGFAVDPTNISRYYVAVASGGV"
     gene            complement(550..1230)
                     /locus_tag="VFH96_11435"
     CDS             complement(550..1230)
                     /locus_tag="VFH96_11435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6974632.1"
                     /translation="MGEMVQFPFAGGNTGGYLATPKQGSGPGVIVIQEWWGLVDHIKD
                     VCDRFAEEGFVAFAPDLYHGKTTKSPDEAGKLMMAMRIDEAERDLSAAVQYLSTQDST
                     TSEKVGVVGFCMGGALSLYTATKNDRVGACVVFYGGHPKVKPDLPNLHAPVLGLYGEN
                     DKGVNPDVVHKLERDVKALGKSIEVVIYPGADHAFFNDQRPQVYNAEAATDAWRRTIA
                     FLREHLST"
     gene            complement(1242..1850)
                     /locus_tag="VFH96_11440"
     CDS             complement(1242..1850)
                     /locus_tag="VFH96_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HET6974633.1"
                     /translation="MEQTKATRLVVGITGATGTIFGVRLLQMLHGSGVETHLVVSKWA
                     ARTLTHETPHSLKYVQSLATQSYSIGAQGAAISSGSFVTLGMVIAPCSMRTLAAIAHG
                     LGDNLIHRAADVILKERRKLVLVVRESPFNEIHLENMLKLARMGVVILPPVPAFYNNP
                     QNLDDMINHITMRVIDQFDIHLDVMNRWDGVLLDRKRATEAE"
     gene            complement(1997..2341)
                     /gene="trxA"
                     /locus_tag="VFH96_11445"
     CDS             complement(1997..2341)
                     /gene="trxA"
                     /locus_tag="VFH96_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET6974634.1"
                     /translation="MSEHVKEVSDSSFEKDVLGSGKPVLVDFWAEWCAPCRMLAPTVA
                     AVAEHYGDTASVVKLNVDDNPSTAQRYGIKGIPTLILFRDGKEVERVVGATSKESITR
                     MIEKYVTVKAVS"
     gene            complement(2397..4058)
                     /gene="rpoD"
                     /locus_tag="VFH96_11450"
     CDS             complement(2397..4058)
                     /gene="rpoD"
                     /locus_tag="VFH96_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET6974635.1"
                     /translation="MNKGEFVNLSEELTDDAVSIEDIDEVLQTLERATSGETEIEEPL
                     IEESSSAVESEEGMNEEVELDLSAGEMDKTSDPIRVYMREMGVVPLLTREQEVSIAKR
                     IERGQKRAEKAIARSPIAVVELFRIGDELAAGALNIRDVVAFSDQMETEEHEDRSEEY
                     LQWTIEGLNNIQTLYKRGLKEYEKFAEELKLSRGKRSKKLLRYRRKLARTRLEIATEI
                     RSLHLKEAARQRLISAIGAVYKETRNLEREIESNNEKLRRKGLKVDKAKEYRKHVANA
                     RKRLKEIEVEHHLSSLEIKRSHQMIAVSEAQTAQAKHELTEANLRLVVSIAKKYQNRG
                     LQFLDLIQEGNLGLMKGVDKFDWRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHM
                     IEVINKLKNTSRELVRELGREPTTEEIAKKMDTSVDKVRKARKLMQDPISLETPIGEN
                     GDSQLGDLIEDKSSESPAARVITSNLREIAGDVLQTLSPREERVIRLRFGLDNGGHER
                     TLEEVGQNFNVTRERIRQIEAKALRKLRHPSRARLLKNFLEGEGQ"
     gene            complement(4125..4682)
                     /locus_tag="VFH96_11455"
     CDS             complement(4125..4682)
                     /locus_tag="VFH96_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET6974636.1"
                     /translation="MSTNSRFAVAVHVLSLMAWSGEEPLKSEQVAESVNTNPVVIRRI
                     LKELAEAGLVVSQTGSLGGSRLAHDPSETTLLDVYQALEYGGVFSLHRAPPSRDCPVG
                     VNIETVLGDVLLEVDTAVEKVLKNISINDVVRRLKPCGAAMASKSPEHIGRNRKRKSY
                     DQKQIALPPLRAGAKSRGNGSSRTA"
     gene            complement(4854..5087)
                     /locus_tag="VFH96_11460"
     CDS             complement(4854..5087)
                     /locus_tag="VFH96_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974637.1"
                     /translation="MSQKKPAFRFAVCISNEDYEASLELHKIYRVIPDEDAEVEGDIR
                     VIDESGEDYLYPVDWFVFIELPQVLEKSLVRAA"
     gene            complement(5389..6450)
                     /locus_tag="VFH96_11465"
     CDS             complement(5389..6450)
                     /locus_tag="VFH96_11465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6974638.1"
                     /translation="MEPELITSLDQINPTWLTKILWRNELLKGGRVAEVSYESDVTSF
                     SVIAHLKISYTRNSQAPDRLFVKFSKPVRPVTTPEGGWEVMFYKFVAPRTPAVLRCYD
                     AVFSREQRKLHLLLEDVSNTHYSEPASQLPPTRRNCELIVRALADIHSAWWGQPPWDV
                     LGWSLPDQAEVDRRIKDVRARIGRFSNFLGDRLTENQRSIYREVLAALPDLYTRLTLP
                     LAYTVVHEDIHIGNVLYPRDPDTDTVRIIDWQTWLIDLAPKDLAHMMALFWFPEQRRA
                     LEIPLLQQYHARLCENGVTDYSWEQLWYDYRLCVIRKLFQPAWQWDTGHEPNIWWNHL
                     ERVMMAYEDLECRELLERR"
     gene            complement(6459..8126)
                     /locus_tag="VFH96_11470"
     CDS             complement(6459..8126)
                     /locus_tag="VFH96_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6974639.1"
                     /translation="MSQSFNQSGNGAPRDEAGGFVAFAHAARLLVVDDEESLRITTAA
                     IFENEGYVVDTASSGDEAIDLMSKEDYDLVLTDLHMEGGDGLSVLNQIRRNAPLTISV
                     VLTGFASVESAIAALQEGAYDYLVKPCDIESMKHTIRRGVEHRRLMLAEQKARADLQQ
                     LNLDLERRIEERTAELKQLNVELADANRAKDVFLATLSHELRTPLTPVVGWIKLLRSG
                     TLDEKSVAQALDAIERNAWLQSRLIDDLLDTSRIATGKLHFEPKPTDLNVAVRAAVDT
                     VRASAGARNIELSMALWPSSLTVMGEPVRLQQIAWNLVSNAIKFTDPGGKVNITTQRD
                     GTNALLTVVDTGVGIEPEFLPHVFDRFRQADGSTSRRHGGLGLGLAIADALAKMHGGY
                     LAAKSDGVGKGATFSMRMELAPTEKVAPEPADTKRHSLEGLEVLIVEDSPDTLLLLST
                     IFRREGANVATASSAAEALSRAIANRPHIIVSDIGMPDVDGYQLLEQLHLLPGLSDVP
                     AIAVSGYASEEDRERALAVGYLALVPKPIDVDVLFGLIHDLKAPAVK"
     gene            complement(8123..10297)
                     /locus_tag="VFH96_11475"
     CDS             complement(8123..10297)
                     /locus_tag="VFH96_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6974640.1"
                     /translation="MGEVPSELAFRPAPSLSEPERPSPRARQYDVFIETTTQMLATPK
                     LQERLLLALEAISTNFGHRQAAIAIINERDAELRIRAAVGFEGDPSATKIEMPLDSSA
                     ACVRVIHDAQPLWISMAEDEASRSLFANLRWQSEVFAVPLFGISEIPSRPGAARVPTQ
                     YWAFEPGARMGVLYVGANQETLDSESITLIKRFAERIGIVASLAAHQERLVSTVTKLQ
                     RERQWIESIMKSVADPIVLTDLDNQILLQNRRAEELFSGSANAGEGKRRALKMNDLLF
                     SAYLSSATVSSSEVIQRDITLVDPIEGSDIHFEVISTPAANSRGEPLGLVSIFRDVTD
                     LREANEELARNFVKLQQAEAESRRERDRLDLIIENVGHPIVVCDSAGNFILFNRRAEL
                     LFQENESVHLSASAAVRTNAVKLTSFISTLASASETGRQAEIELIDPEDGRALPMEIT
                     AREVLDATGQVTAVVSILHDLTEIRELERRRVEQQLFESEKLAAVGRLAASIAHEVNN
                     PLEAIKNALYLMQAGGEGDKNSRFLEIARKETERVSHIIRQMLGFARRPGEVDWVDVN
                     QLLEETLVLLEKKMRQLRIRITKSFDDSLPRVRARADQLRQVFLNLIINAQQAIEGDG
                     EIVISTSRYEQALQPSILVQLKDSGVGIAEDDLTRIFDPFFSTGKKGTGLGLWVTQDI
                     VRQHGGRIEVSSEIGRGTVFNIVLQVESAILEEQSETKSTTR"
     gene            complement(10348..10626)
                     /gene="gvpA"
                     /locus_tag="VFH96_11480"
     CDS             complement(10348..10626)
                     /gene="gvpA"
                     /locus_tag="VFH96_11480"
                     /inference="COORDINATES: protein motif:HMM:NF006874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_component: GO:0033172 - gas vesicle shell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle structural protein GvpA"
                     /protein_id="HET6974641.1"
                     /translation="MAVERAPGGTSLIDVLDRVLDKGIVIDAWVRISLVGIDLITIEA
                     RVVVASIDTYLKYAEAVGLTPAVSRPAITPGGETIETETRRRETNVTR"
     gene            complement(10724..11122)
                     /locus_tag="VFH96_11485"
     CDS             complement(10724..11122)
                     /locus_tag="VFH96_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HET6974642.1"
                     /translation="MSSERSTLITARPPGLVDVLDRILDKGLVIAGDIKVSLANVELL
                     TLQIRLLVCSIDKAEQIGLNWWRYDTNLTTRAERAEAENVELRERLTELEQEIKKLAA
                     ANIAAVRSESTEVVTSRRAGRKATTKRRTK"
     gene            complement(11214..11579)
                     /locus_tag="VFH96_11490"
     CDS             complement(11214..11579)
                     /locus_tag="VFH96_11490"
                     /inference="COORDINATES: protein motif:HMM:NF016977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HET6974643.1"
                     /translation="MSFSPENPLSAKPEIETSHADEFVEQLRVVTDTLPARINIDAQS
                     VEQGLAKLVLTLIEFIRRLLEKQAVRRMEGGDLSPEQIEELGLALMRLEAKLQELKTQ
                     FGLEAEDLNLDLGPIGRLI"
     gene            complement(11579..12298)
                     /locus_tag="VFH96_11495"
     CDS             complement(11579..12298)
                     /locus_tag="VFH96_11495"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HET6974644.1"
                     /translation="MRLYVYCLAEGVERLNKTLPGVSGAPVRIVEFDEDLSALVSVYR
                     PEDFQVSRKNALAHHEVVRSITEQTTPLPARFGTVVKIEQLRNYVSTHHEAIKAKLAH
                     VRGGVEMNVRMIGTITANTSQEPENPGGPGTAFLLEKRREILRNEAGAAQKGHLSAWL
                     RDKLGDLIKDEKISVIPSKTVILARADHLINRVNVQEYRTKMSKAVAERPEIRFMVSG
                     PWPPYSFANIELEFSRQFGVS"
     gene            complement(12295..12516)
                     /locus_tag="VFH96_11500"
     CDS             complement(12295..12516)
                     /locus_tag="VFH96_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HET6974645.1"
                     /translation="MSDFDQIIAGEELENTDLSLLETLDHVLNRGLVIAGEITIAVAD
                     VDLIFVGLNVMVSSVETANEVLRRREATE"
     gene            complement(12520..13326)
                     /locus_tag="VFH96_11505"
     CDS             complement(12520..13326)
                     /locus_tag="VFH96_11505"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HET6974646.1"
                     /translation="MADLAYYVYCIAESAAAAQLPVDSLPAAIEEDSKLKWISVNNTL
                     AALASKVPRATYSEDNLAEQLTDATWTAIRAMRHETVVEFVAKRATVIPLRFGTIYLE
                     RSGIEQMLASKGRELESIIEQLRGREEWGVNVYYDRAVLLNSITSVSPVLRDLVQRAE
                     QAPPGQSYLMQKKIEALKIDEARTAVNRIVDQIEETLKTQTDDARRLRILKVETTEHG
                     ELKAKFAFLVKRAAYEEFRDAAERLAQEHQSAGVRLELTGPWPVYNFCTG"
     gene            complement(13332..15299)
                     /locus_tag="VFH96_11510"
     CDS             complement(13332..15299)
                     /locus_tag="VFH96_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HET6974647.1"
                     /translation="MNARYLFFGGKGGVGKTTAASATALYLLNKLKKDESILLFSTDP
                     AHSLSDSLDVKIGNRLREVKQHRGARLFAYEMDASLALERFRAEHGKVLAEIAERGTL
                     LDEDDINELLSLSLPGMDEVMSLFELSELDREGKYAHIVVDTAPSGHTSRLLRLPEVF
                     DRMVKALDLMGDKHRYIVAHFARRRPVADEVDVFLQDLSRRIEAVRKLLHDREQTSFT
                     LVTIPEAMAVRETGRYLTLLSEQGVPVRDLIVNRVEQEHGTCKYCRARVRSQRPWIKE
                     IGSLFGDLRIRYVPLMAKEVRGVEELKRIGKLIWTEEMKPRMTRIKNPDFKSVSSVAQ
                     SSFPLKPRKIVIFGGKGGVGKTTAAAAFAQALAQANPKEKILVFSTDPAHSLSDSFDE
                     EIGEQENGVAGHANLDAMEIDPGKWFEELKERYRTWTDELFASLSGGSRMEIKFDREA
                     MRELVELTPPGIDEIAALGTISDLLDLEQYQTLVLDTAPTGHLIRFLELPQVALSWIR
                     TFIKLLLKYKDVMRANQVAEELVAMSKSIKKVIALLTDPERCEFVGVAIPERMSLEET
                     IDLAKSLEKLKVPMSKLLINGVVPVNDCKFCQSRRKMQEQVMKEFQTKFRRRSVEIFV
                     APQQPHEIRSSKNLRGHFSAWEKYSAVRERI"
     gene            complement(15296..15622)
                     /locus_tag="VFH96_11515"
     CDS             complement(15296..15622)
                     /locus_tag="VFH96_11515"
                     /inference="COORDINATES: protein motif:HMM:NF016976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HET6974648.1"
                     /translation="MFLIDDLLLAPVNGFKFILGQIQKMADQELNDDSVIKEQLLELQ
                     MRYELEEISDEEFREREAELFARLRVIKMRQLDLMHEATAHTAESSSFVIEIGGDDQG
                     EFRDKG"
     gene            complement(15622..16440)
                     /locus_tag="VFH96_11520"
     CDS             complement(15622..16440)
                     /locus_tag="VFH96_11520"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HET6974649.1"
                     /translation="MSGAEQTPAVETVTEGKYVYCIIETSEPRSFGPMGIGARGDDVY
                     TIHHKGLAAVVSNTPLVVYDPTRENVFAHEQVNETVMREFTVLPMAFGALFRTEEDIV
                     ELIRGTYDALRDVLTKMEGKVEFGLKVNWDRDKVTAELEEKDDEIRHLKEQIVSRTTG
                     STYFARMQLGRLIETALTDQADAYIREVYSQLRDTAVASRANKPIGDRMIMNAAFLIE
                     RDREAEFDQKVKEIASKYEGKLSFKYTGPWPPYNFVHIRLKLERGEGATEAVAS"
     gene            16723..16869
                     /locus_tag="VFH96_11525"
     CDS             16723..16869
                     /locus_tag="VFH96_11525"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET6974650.1"
                     /translation="MLWTILVILLVLWLIGLLSHVGGGLIHLLLVVAVIILVINLVTG
                     RRAV"
     gene            complement(16925..18406)
                     /locus_tag="VFH96_11530"
     CDS             complement(16925..18406)
                     /locus_tag="VFH96_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6974651.1"
                     /translation="MATADTPSQSRLKESIRTDVCIIGAGIAGLTIAYLLAKEGRSVV
                     VLDDGVSGGGMTGRTTAHLTNAYDDRYLEIEKLHSAEGARLTAQSHSAAIEKISQIIA
                     AEKIDCDFEWLDGFLFCAPEHSVDLLDDELAASHRAGLNGVEKVARAPVESFNTGPAL
                     RFPRQAQFHPLKYLDGLVKAILREDGRIFGQTHATKIEGGAQAIVETSHGPVVKSEVV
                     VVATNTPVNDRVAIHTKQAPYITYVIGMRVPKDSVTRALYWDTGDPYHYLRLQRENDH
                     DVLIVGGEDHKTGQANDGNERFARLEQWTRERFPQSLEVEYRWSGQVMEPVDGLAFIG
                     RNPLDEANVYIATGDSGQGMTHGTIAGMLLTDLIQGRKNPWEDLYSPSRLRLKSLPEY
                     ASENINVAGQYADYVTAGDIKSESELRPGAGAIMREGVSKIAVRRDENGNVHKLSAVC
                     PHLGCIVAWNATEQTWDCPCHGSRFSASGRVYQGPANSDLSKP"
     gene            complement(18467..18622)
                     /locus_tag="VFH96_11535"
     CDS             complement(18467..18622)
                     /locus_tag="VFH96_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974652.1"
                     /translation="MADNASSTASTGIIAVLVIFVILVVAALFVFGGRWFGGNKRIDV
                     NIGTPAK"
     gene            complement(18686..19579)
                     /locus_tag="VFH96_11540"
     CDS             complement(18686..19579)
                     /locus_tag="VFH96_11540"
                     /inference="COORDINATES: protein motif:HMM:NF005214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6974653.1"
                     /translation="MASKKGSKGKLKQPKSPMPPQHQQKPGVEAKLKPRPNYQAPMYQ
                     GAGKLKEKVALITGGDSGIGRAVAVLFAREGADVAFTFLKKEKRDADETKRAIEAEGQ
                     QAFALTGDVRDPKVCQKLVKQTVDKFGKLDILVNNAAFQQHQESLEDVTEEQWDRTFK
                     TNIYGYFYMTKAALEHLEEGSAIVNTGSITGLEGSKELLDYASTKGAIHAFTKSLAQN
                     LVERGIRVNCVAPGPVWTPLNPADKKAKDVAEFGADTPMKRPAQPEEIAPAYVYFASE
                     VDSSYVTGEVLTLLGGETTAG"
     gene            19730..19927
                     /locus_tag="VFH96_11545"
     CDS             19730..19927
                     /locus_tag="VFH96_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974654.1"
                     /translation="MAKKPAKSHRELIDTGTDKRFVRRNDKGQFDEVVDVGHSLAQDR
                     KQEAKTKVPPGQGDRGDQKTK"
     gene            complement(19977..20165)
                     /locus_tag="VFH96_11550"
     CDS             complement(19977..20165)
                     /locus_tag="VFH96_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974655.1"
                     /translation="MSKRIDPLKPKYDIICSWCGALIRSTNMELPEQMCLICHARMLN
                     DYFQRINKKPRIHADKRG"
     gene            20220..21233
                     /locus_tag="VFH96_11555"
     CDS             20220..21233
                     /locus_tag="VFH96_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974656.1"
                     /translation="MTKLERLQNIGIRRLGTPKRGFRYKPVSGRLTKADVDRINDLKI
                     PPAWTEVFINPAANGRVQVIGKDTAGRWQYLYHESHTRTQELRKFRRITKFAKAIPQM
                     RATVARHLRQPGLSRERVLAAVLRILSTCYMRPGSEVYASEHGSYGIATLRRKHVTVK
                     GERIAFDFPGKSGVRQQRELRDRQVARVIRETIKLPGYNVFKYINGDDKPVTVTRRHI
                     NEYIKEVMGSSFSAKDFRTWAGTLVCACALARNGVEIPEKKTARNKRIVEAIKETAEV
                     LGNTPAVCRGSYVCPEIISSFEKGQVIDARATNLEDLIGFRGRGLHKAEQSLLKFLKK
                     GVK"
     gene            complement(21484..22554)
                     /locus_tag="VFH96_11560"
     CDS             complement(21484..22554)
                     /locus_tag="VFH96_11560"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6974657.1"
                     /translation="MRFIEKFFKNELAIDLGTVNTLIYQPGKGILLNEPSVVALDRYD
                     GEVVSVGHKAVKLLGREPRGIEVHRPIRSGTIFNFEVTRKMLRSFIGQVTDGIRFGHF
                     VVGIPGSATAVEQRSVRDAAHDAGGRRTDLVDEGLAAGIGAGLAFDDERAHLVVDIGG
                     GTTNIAIVASGGVVSSTSLQAAGNAMDESIRDYVRSNYCLQIGEYTAEALKRELGALS
                     DDDIPSDERKIQLVGKQLSDGYACAVQITAEEVREALEPTINEIVTGIRRVIEDAQPE
                     AVADIYYSGMILTGGGSLLKGLKSRLQNELKLRATVPEDPISTVVMGAGILLDRPDHL
                     YRCAIRPNLPVWQEAEELQVSW"
BASE COUNT         5013 a         6569 c         5906 g         5409 t
ORIGIN      
        1 caaacaccgc ctgatgcgac tgcaacgtaa tagcgcgaga tgtttgtcgg atcgactgca
       61 aatccaacga cgcgtccgga agtgaaggct ggaccaatcg agcgcattct gagtccgttg
      121 aaggtcggag tcgacatcgg atcgcgcggt gttggtgatg cttcgggcgt tggcgaaggg
      181 ctcgctgttg cggttggaga tggcgacggt gtcgccggcg gtctgggggg ttcctgcgca
      241 actacgcgca gcggcagtag aaacaggagc gagatcacag tcaatgcgca cgaagacatg
      301 gtcataaaca gtttcctctt cggttgtgat agagatggag cttgtaaaag cctgcgaagc
      361 aggtgaaagc ataaagcctg ggcgccagcc ccaggaaaag attagaaaaa taaattcaga
      421 gcccgccagg cgggcgacag cgcgtaatgc aaaatggctg tcgcccgtta tcacgggctc
      481 gactcgctca acgatcgttg atcctggggc tggcgcccca ggctttatgc tttcgtccgc
      541 ttcgcggact cacgtggata aatgctcgcg aaggaacgcg attgtgcgcc gccacgcatc
      601 ggtcgcagct tcggcgttat aaacctgcgg gcgctgatcg ttaaagaagg cgtgatcggc
      661 gccggggtaa attacgacct caatcgattt gccgagcgct ttcacgtcgc gctccaactt
      721 atgaaccaca tcaggattta cgcccttgtc gttctcaccg taaagaccca gcaccggggc
      781 gtgcagattc ggcagatcgg gttttacttt cggatgtccg ccgtagaaaa caacacacgc
      841 accgacacga tcgttctttg tcgctgtgta aagcgaaagc gcaccgccca tacagaagcc
      901 aacgacgcca accttttcac tcgtggttga gtcttgcgtg gaaagatact gaacggcagc
      961 gctgagatct ctctcagcct cgtcgatgcg catcgccatc atcagcttac cggcttcgtc
     1021 tggagacttc gtcgttttcc cgtgataaag atcaggcgcg aacgcgacga agccttcctc
     1081 ggcgaatcga tcgcaaacgt ctttgatgtg atcaacgagt ccccaccact cctgaatcac
     1141 tatcacgccc gggccgctgc cctgtttcgg tgtcgcgaga tacccgccgg tgtttcctcc
     1201 ggcaaacggg aattggacca tttcacccat gacttgctct cctactctgc ttctgtcgct
     1261 cgctttctgt ccagcaacac gccatcccag cgattcatca cgtcgagatg gatatcgaat
     1321 tgatcgatga cgcgcatcgt gatgtgattg atcatgtcat caagattttg agggttattg
     1381 tagaacgcgg gcaccggcgg cagaatcacc acgcccatgc gcgcgagttt caacatgttc
     1441 tcgagatgga tttcattgaa cggcgactcg cgcacgacga gcacgagctt gcgtcgctct
     1501 ttgagaatca cgtcggcagc gcgatgaatt aagttgtcgc cgagtccgtg cgcgatcgcg
     1561 gccagcgtgc gcatgctgca cggcgcgatc accattccga gcgttacaaa cgagccgctc
     1621 gagatcgcgg ctccctgcgc gccgatactg tagctctgcg tcgcgaggct ttgtacgtac
     1681 ttgagtgagt gtggggtttc atgtgtgagc gtgcgcgccg cccacttact cacgacgaga
     1741 tgcgtctcca ctcccgaccc atgcagcatc tgcaaaagtc gcacaccaaa gatcgttcca
     1801 gtcgccccgg tgatccctac cacaagccgc gtggctttcg tttgttccat gcggtctcat
     1861 tttagccaca aagaggcaca aaaagcacac aaagctatcc acagatggcg cagatttgtt
     1921 atgtcatcta tgtaatctgt ggataaaaaa agccggacgc catagacgcc cggccttttc
     1981 cgcccagagt gttttcttaa cttacagcct tcactgtcac gtacttctcg atcatcctcg
     2041 tgatcgattc tttactggtc gcgccgacaa cgcgctcgac ttcttttcca tcgcggaaaa
     2101 gaatcagtgt cgggatacct ttgataccgt aacgttgtgc agttgacggg ttgtcgtcaa
     2161 cgttcaactt caccacgctc gcggtgtcgc cataatgctc tgcgacagcc gcaacggtcg
     2221 gcgccagcat tcgacaaggg gcacaccact cagcccagaa atctaccagt accggtttac
     2281 cggagcccaa tacgtctttt tcaaacgagc tgtcgctcac ttccttcaca tgttcactca
     2341 ttttttagtt gctcctaatt tgctaacccg gcgctacgca cgctcggtta aaatatttac
     2401 tgcccctcgc cttcgaggaa attcttgagt agccgcgccc gcgacgggtg acgcagtttg
     2461 cgcagcgcct tggcttcaat ctggcgaatg cgttcgcgcg tcacgttgaa gttctggccc
     2521 acttcttcca gcgtccgttc gtgaccgccg ttgtcgagtc cgaatcgcaa acgaatcaca
     2581 cgctcttcac gcggactcaa cgtctgcaac acatcgcctg cgatctcgcg caagtttgaa
     2641 gtgatgacgc gcgctgccgg gctctcgctc gatttgtctt caatcaagtc gccgagctgc
     2701 gaatcgccat tttcaccaat cggcgtctcc agcgagatcg ggtcctgcat caacttgcgc
     2761 gccttgcgga ccttatctac tgaagtatcc atcttctttg cgatctcttc agtcgttggt
     2821 tcgcggccaa gctcgcgcac cagctcgcgc gaagtgttct tgagcttgtt gatgacctcg
     2881 atcatgtgca ccggaatacg aatggtgcgc gcctgatcag cgatagcacg cgtgattgcc
     2941 tggcgaatcc accaggttgc ataagttgaa aacttataac cacggcgcca gtcaaactta
     3001 tcgacgccct tcatcaaacc aagattgcct tcctgaatca gatccagaaa ctgcaaacca
     3061 cggttctgat atttcttcgc aatcgaaacc accaggcgca ggttagcttc cgtcagctcg
     3121 tgcttcgcct gtgccgtctg cgcttcgctg acagcaatca tctggtgcga acgtttgatc
     3181 tcgagcgacg acaggtgatg ctccacctcg atctctttca aacgcttgcg cgcgttggct
     3241 acatgcttcc ggtattcctt cgccttgtct accttcaaac ctttgcgccg cagcttctcg
     3301 ttattcgact cgatctcgcg ttcgaggttg cgcgtctctt tgtagactgc gccgatggcg
     3361 gagatgagtc gctgccgggc agcttccttc aggtgcagac ttctgatctc ggtggcgatc
     3421 tccagacgag tgcgggccag cttgcgccgg tagcgtagaa gtttcttcga acgcttgcct
     3481 cgcgaaagtt tcagctcttc ggcgaatttc tcgtattctt ttagccctcg cttgtagaga
     3541 gtctgaatgt tgttaagacc ttcgatcgtc cactgcaaat attcttcact acgatcctcg
     3601 tgctcctcgg tctccatctg atccgaaaaa gccacaacgt cgcggatgtt gagcgcgccg
     3661 gcagccagct cgtcaccaat ccggaaaagc tcaactactg caattggaga acgtgcgatc
     3721 gctttctccg cacgcttttg gccgcgctcg atgcgcttcg caatcgacac ttcctgttcg
     3781 cgcgtaagca acggcacaac acccatctcg cgcatgtaaa cgcggatggg atccgacgtc
     3841 ttatccattt cgccggctga gaggtcgagc tcgacctcct cgttcattcc ctcttcggat
     3901 tcgacggcag acgacgactc ctcgatcaaa ggctcctcga tctcggtttc accactggta
     3961 gcccgctcga gcgtttgtag tacctcgtcg atatcttcaa tcgacacggc gtcgtccgtc
     4021 agttcctcac tgaggttgac gaattcgcct ttattcatga gctcgatacc gctttgatct
     4081 ttttcaagcc tggacaaatt aatattcctt tctcgcggtt ttaattacgc agttcgggag
     4141 gatccgttcc cccggctctt cgccccggct cgcaatgggg gcaaagctat ctgcttctgg
     4201 tcgtatgatt tcctcttccg atttctccca atatgctctg gcgacttcga tgccatagca
     4261 gcgccacaag gtttcaagcg ccgcacgaca tcattaatcg atatgttttt caatactttt
     4321 tcaaccgcag tatcaacttc cagcagcacg tctcccaaaa cggtttcgat attgacgccg
     4381 accgggcaat cgcgactcgg aggtgcgcga tgcagcgaaa agacgccgcc atattcgagt
     4441 gcctggtaaa cgtcgagcag ggtggtttcg gaggggtcat gcgccagacg agagccgcct
     4501 agcgaaccgg tttgcgacac caccagcccc gcttcggcaa gctccttcag gatgcgccgg
     4561 atcacgaccg gattggtgtt tacgctttct gcaacctgtt ctgatttaag gggttcttcg
     4621 cctgaccagg ccatcaaact taaaacatgt accgctaccg cgaacctact atttgtcgac
     4681 atatcaaagc tatctaagga aacacgagcc gccgttttgt aaccattata gttacaaatt
     4741 tgattttgtc aactatttgt gtgtgttagg gtttgaggtg gaaggacaca gattgggatc
     4801 gggaagacag ttaaatcaga gggtaacgcg agactaaatc gttcaaacct gaatcaagct
     4861 gctcgtacca gagacttttc gagcacctga ggcaattcga tgaatacaaa ccaatcgacg
     4921 ggatagagat aatcttcgcc gctctcatca attactcgaa tatcgccctc cacttcggca
     4981 tcttcatcgg ggatcactcg atagatttta tgaagctcta gtgatgcttc gtagtcttca
     5041 ttactaatac aaaccgcgaa tctaaaagct ggcttctttt gactcataac ttaatccaaa
     5101 tagcgctttc gtttgatttg ttttcgacca ataccgtgcg cttcatacca atgcaattca
     5161 gctaaccgta ttctaccgct tctgagtcga acctgcgcta tcccttttaa ctttcgccaa
     5221 cgaccgggac cgtacttacg tcgcaaacga gcgagttctc gaatcttcgt accgactgca
     5281 atgatttcga tatcagtaat ctcactgacg atctcaaagt ccatctgcaa ctcatttaat
     5341 caaggacggt aaaaggactc aaggcaagat gcgagttttt cggagtctct atctccgctc
     5401 gaggagttcg cggcattcca agtcttcgta cgccatcatc acgcgttcca agtggttcca
     5461 ccagatgttc ggctcgtggc cggtgtccca ctgccaggcg ggctggaaga gttttcggat
     5521 tacgcagaga cggtagtcgt accataactg ctcccaggaa tagtccgtca ctccgttctc
     5581 acagagccga gcgtggtatt gctggagaag cggaatttcg agagcccgtc tctgctcggg
     5641 gaaccagaag agcgccatca tgtgggcgag gtctttcggg gcgagatcga tgagccaggt
     5701 ctgccagtcg atgatgcgaa ctgtgtctgt atcaggatca cgagggtaga gaacgttgcc
     5761 gatatgaatg tcttcgtgaa ccacagtgta cgctaacgga agcgtcaggc gggtgtataa
     5821 atcaggtaat gccgcaagca cctcgcgata aatactgcgc tggttttcgg taagccgatc
     5881 gccgaggaag ttgctaaagc gaccgatacg cgcgcgaacg tcctttatcc ttcgatcaac
     5941 ttcagcctga tcaggaagag accagccgag cacatcccac ggcggctgtc cccaccaggc
     6001 ggaatggata tctgccaacg ctcgaactat tagctcgcaa ttccgccgcg taggtggtaa
     6061 ctgggaagct ggttcactgt aatgcgtgtt tgaaacatcc tctaatagca aatgtaactt
     6121 gcgttgttcc cgtgaaaaca cagcatcgta acagcgaagt acggcgggag tgcgcggggc
     6181 gacgaattta taaaacatca cttcccaacc accctccgga gttgtgacgg gacgcactgg
     6241 ctttgagaac ttcacgaaga ggcgatccgg tgcttgtgaa ttacgtgtgt atgagatttt
     6301 gaggtgagca atgactgaga agctcgtcac gtcggattca taactgactt ccgcgacacg
     6361 gcctcccttc aatagctcat ttcgccagag aattttggtc agccacgtcg ggttgatctg
     6421 atcgagactg gttatgagtt caggctccaa agtgcttgct acttaacggc cggggctttg
     6481 aggtcgtgaa tcagaccgaa gagaacgtcg acgtcgatgg gtttgggaac gagggcgaga
     6541 tagccgacgg caagggcgcg ctcgcggtcc tcttcacttg cataacccga caccgcaatt
     6601 gccggcacat cgctcaggcc gggaaggagg tggagttgtt cgagaagttg atagccgtct
     6661 acatccggca tgccgatatc tgaaacgatg atgtgcggac ggtttgcgat agcgcgactc
     6721 agcgcctccg ccgctgaaga agcagtcgcc acgttcgcgc cttcgcgacg aaagatcgtg
     6781 ctcaacagca gcaacgtgtc aggcgagtct tcgacgatca ggacttcgag accttcgagt
     6841 gaatgacgtt tcgtgtctgc cggctcggga gcgacttttt cagtcggcgc gagctccatt
     6901 cgcatcgaga atgtggcgcc ttttcccacc ccgtccgact tggctgcaag atatccgcca
     6961 tgcatctttg cgagtgcatc ggcgatcgcg agtccgagac ctaaaccacc gtgtcgtcgc
     7021 gaagtcgagc cgtctgcctg ccggaaacga tcgaacacgt gcggtaaaaa ttcaggctcg
     7081 ataccaacgc cggtatcaac cacggtcagt aaagcgttgg tgccgtcgcg ctgtgttgta
     7141 atgtttactt tgccgccggg atcggtgaac ttaatcgcat tcgaaacgag gttccacgcg
     7201 atctgctgta aacgaaccgg ctcacccatc actgtcaaac tcgatggcca caacgccatc
     7261 gacagctcga tattccgcgc cccagcagac gcgcgcacag tatcaaccgc agccctgact
     7321 gcaacgttca aatccgtcgg cttcggctcg aagtgaagtt tgccggtggc gatgcgagac
     7381 gtgtcgagca gatcgtcaat caatctcgac tgcagccacg catttcgctc gatcgcatca
     7441 agggcctgcg ccacactctt ctcgtcgagc gttccgctac gcaacaattt gatccatccg
     7501 actacgggcg tgagcggcgt gcggagctcg tgagacagtg ttgccagaaa cacgtctttg
     7561 gcgcgattag cgtcagcgag ctcgacgttg agttgtttca actccgcggt ccgctcttca
     7621 attctccgct cgagatcgag attcaactgt tgcagatccg cgcgtgcttt ctgttcggcg
     7681 agcatcagcc ggcggtgctc gacgcctctg cgaatcgtgt gcttcatgct ctcgatgtcg
     7741 cagggcttta cgaggtagtc gtaagcacct tcctgcaacg cggcaatcgc cgactcgaca
     7801 gaagcaaatc cggtcagcac aacggagatc gtgagcggtg cgttgcggcg gatctgattg
     7861 agaacggaga gaccgtcgcc accttccatg tgcaggtcag tgagcacgag gtcatagtct
     7921 tctttactca tcagatctat ggcttcgtca ccactcgatg cggtgtcaac gacgtaacct
     7981 tcattctcaa agatcgcagc cgttgtgata cgcagacttt cctcgtcgtc tacgaccagc
     8041 aatcgcgccg catgggcaaa cgcaacaaag ccgcccgcct catcgcgagg cgcgccgttt
     8101 cccgattgat tgaatgattg actcatctcg tcgtcgattt agtttcgctt tgttcttcga
     8161 gaatcgcgga ctcaacctga agcacgatat taaaaaccgt gcctctacca atctcactgc
     8221 tcacttcgat gcgaccaccg tgttgcctca cgatgtcctg cgtgacccac aagccgagcc
     8281 cggtcccttt cttgcccgtg gaaaagaacg gatcaaaaat gcgcgtcaga tcgtcctcag
     8341 cgatcccgac gccgctatcc ttgagttgca ctaaaatcga cggctgcaat gcctgttcat
     8401 aacgcgaagt ggaaataaca atctcgccgt caccctcaat cgcctgctgt gcattgatga
     8461 tcagattcag aaatacctgt cgtaattgat cggcgcgcgc tctcacgcgt ggcagcgagt
     8521 cgtcaaacga tttagttatc ctgatgcgca gttggcgcat tttcttttcc agaagtacga
     8581 gggtctcttc aagcaactgg ttaacgtcga cccagtcaac ctctcccggc cggcgcgcaa
     8641 agccaagcat ttggcgaatg atgtgcgaca cgcgctccgt ctcttttctc gcaatttcga
     8701 ggaagcgcga gttcttatca ccttcgccac cagcctgcat caggtaaagc gcgttcttga
     8761 tcgcttcgag cggattgttc acctcatgcg cgatcgacgc cgcgagacgc ccgacagccg
     8821 ccagcttctc tgattcaaat agctgctgct cgacgcgccg tcgctcaagc tcacgaatct
     8881 cagtcaggtc atgaagaatt gagacgacgg cagtcacctg tcccgttgca tccaaaacct
     8941 cgcgtgcggt gatctccatc ggcaacgccc gaccgtcttc gggatcgatc aattcaatct
     9001 ctgcctggcg tccggtttca gaagctgatg cgagcgtgga gatgaacgac gtgagcttga
     9061 ctgcattcgt gcgcaccgca gcagatgccg acaagtggac tgattcattc tcctgaaaca
     9121 acaactccgc gcgacggttg aacaggatga agttacccgc cgagtcgcac acgacgatcg
     9181 gatgacccac gttctcgata atcagatcga gacgatcgcg ctcgcgacgt gattcagctt
     9241 ccgcttgttg cagcttgacg aagttgcgcg caagctcttc attcgcttcg cgcagatccg
     9301 tgacgtcgcg gaagatcgag acgagaccga gcggctcacc gcgtgaattg gcggcgggtg
     9361 tcgagattac ttcgaagtga atgtccgagc cttcgatcgg atcgacgagc gtgatgtctc
     9421 gctgaataac ttcagacgaa gaaactgtgg cactcgaaag ataagcagag aacaaaaggt
     9481 cgttcatctt caacgcgcgg cgcttacctt caccagcgtt ggcggaaccg ctgaacaatt
     9541 cttcagcacg tcgattctgt aaaagaatct ggttgtcgag atccgttagc acgatcggat
     9601 cggcgaccga cttcatgatc gactcaatcc actgtcgctc gcgctggagc ttcgtgacag
     9661 tcgagacaag tcgttcctgg tgcgcggcaa gtgaggctac tatgccaatc cgttcggcga
     9721 atcgcttgat cagagttatc gactccgaat caagcgtctc ctggttcgcg ccaacgtaca
     9781 acacacccat tctcgcgccc ggctcaaagg cccagtattg agtcggaact cttgcggcac
     9841 cgggacgcga tggaatctcc gagataccga acagtgggac ggcgaatact tcagactgcc
     9901 agcgcaaatt agcaaagaga cttcgtgagg cttcgtcttc tgccatcgag atccacaacg
     9961 gctgagcatc gtgaatcaca cgcacacacg ccgcgctcga atcgagcggc atttcaatct
    10021 tggtcgctga gggatctcct tcaaaaccaa cggctgcgcg aatgcgcaac tcggcgtcgc
    10081 gctcgttgat gatcgcgatc gctgcctgcc ggtggccgaa gttggtcgat atcgcttcca
    10141 gagcgaggag gagtcgctcc tgaagcttgg gcgtggccag catttgcgta gtcgtctcga
    10201 taaagacgtc atactggcgc gcgcgcgggc tgggccgctc aggctccgaa agggatgggg
    10261 ccggtcgaaa agctagttct gagggaactt ctcccataaa agattcccga tgttctatga
    10321 ccgtctactc agtcgagtag cgttgcttca cctcgtgacg ttggtctcgc ggcgccgagt
    10381 ctcggtttct atcgtttcac cacccggcgt tatggcgggt cgggaaactg ccggtgtcag
    10441 gcccacggct tcagcgtact tcaagtacgt atcgattgag gcaacaacta cgcgcgcttc
    10501 gatggtaatc aggtcaatac ccaccagcga gattctcacc catgcgtcga tcacgatacc
    10561 tttgtcgagt acgcgatcaa ggacgtcaat taagctggtt ccgcctggtg ctctttcgac
    10621 agccatttgc ttcggttcct ttattagaag tgttatacga gctcacgcga ggtttcacgg
    10681 cgcccaactg ctcttctcaa aaaacctttc tcattgcttc cgattactta gtccggcgct
    10741 ttgttgttgc tttgcgcccc gcacgacggc tagtaacaac ttcggtcgac tcgctacgca
    10801 ccgcagcgat attcgcagcg gcaagctttt taatctcctg ttcaagctcg gtcagccgtt
    10861 cgcgcaattc aacgttttcc gcttcggcgc gctcggcgcg tgtcgtcagg ttggtgtcgt
    10921 agcgccacca gttcaaacca atctgctcgg ccttgtcgat cgagcaaacc agcagacgaa
    10981 tctgcagagt caatagctct acattcgcca acgacacttt gatgtcgcca gcgatcacaa
    11041 gtcctttatc aagaatacgg tcgaggacat caaccaggcc cgggggacgg gccgttatca
    11101 gggtagaccg ctcgctggac actttcgccc tccactatca gggggcggct ttactcaaaa
    11161 aactcagatg gccgccacca tcgtatgact cctgccgagc aagatgcgta ctgctaaatc
    11221 aagcgcccga ttggaccgag atcgagattg aggtcttccg cttctaatcc gaactgagtt
    11281 ttcaactcct gaagtttggc ctccaacctc atgagcgcca atcctaattc ttcgatctgt
    11341 tcaggtgaga gatccccgcc ttccatgcgg cgcaccgcct gtttttccaa cagacggcgg
    11401 atgaactcaa tcaaagttag tactagcttc gccagaccct gctcgacgct ctgcgcatcg
    11461 atattaattc gcgcggggag ggtgtccgtg acaacacgaa gttgctccac aaactcatcc
    11521 gcgtgcgaag tttcgatctc cggctttgcc gaaaggggat tttcggggga aaacgacatc
    11581 agcttactcc gaactgcctg ctaaactcaa gctcaatgtt tgcaaaacta tacggcggcc
    11641 acggaccgct caccataaag cgaatctcgg gccgttccgc aactgccttg gacatcttcg
    11701 tccgatactc ctgaacgttt acacgattaa tgaggtgatc cgccctggcc aaaattacgg
    11761 ttttagaggg gattacagag attttctcgt cttttatgag atcgcccaac ttgtctcgca
    11821 gccatgcgga taaatgccct ttttgagctg cgccggcctc gttgcgaagg atctccctac
    11881 gcttctccag caggaatgcg gtgccgggtc cgcccgggtt ttcgggctcc tgagatgtgt
    11941 tggcggtgat tgtgccgatc attctcacgt tcatctcaac gccgccacgc acgtgggcga
    12001 gtttggcttt gatcgcttcg tgatgcgttg aaacgtaatt ccggagctgt tcaattttga
    12061 cgaccgttcc gaagcgagcg ggcaatggcg tcgtttgctc cgtgatactg cgcacgacct
    12121 cgtgatgggc aagcgcgttt ttgcggctga cctgaaaatc ctcaggtcgg taaacactca
    12181 cgagagccga aaggtcttca tcgaattcaa cgattcgtac cggggcaccg gaaacaccgg
    12241 gcaacgtctt gtttagccgc tcgacacctt cggcaaggca gtaaacgtat agcctcattc
    12301 cgttgcttcg cgcctgcgta agacttcgtt tgcagtttcg accgagctca ccatcacgtt
    12361 caggcccacg aagatcagat cgacgtctgc gacggcgatg gtgatctcac cggcgatcac
    12421 gaggccgcga ttgagcacgt gatcgagtgt ttcgagtaaa gagagatcgg tgttttccaa
    12481 ctcctcgccg gcgatgatct gatcgaagtc tgacatgttt tatcctgtac agaaattgta
    12541 gaccggccac ggacctgtta gttccaggcg aacgccggcg gactggtgct cctgcgcgag
    12601 acgctccgcc gcatcgcgaa actcttcgta cgccgcgcgc tttacgagaa atgcaaactt
    12661 agctttcagc tcaccgtgct cggttgtttc aacctttaga atgcgcaaac gccgcgcgtc
    12721 gtcggtttgc gtttttaatg tctcttcgat ctgatcgacg attcgattca ctgctgtgcg
    12781 cgcttcgtcg atcttcaagg cttcaatctt cttttgcatc aggtacgact gtcccggcgg
    12841 cgcctgctcg gcacgctgga ccagatcgcg tagcactggg ctaacggaag tgatgctatt
    12901 taacaaaaca gcgcgatcgt agtagacatt cacgccccac tcttcgcggc cacgcagttg
    12961 ttcgatgatc gattcgagct cgcggccctt ggacgctagc atctgctcga tgccgctgcg
    13021 ttcgaggtag atcgttccga agcggagcgg gatcacggtg gcgcgcttcg caacaaactc
    13081 gacgactgtc tcgtgtcgca tcgcgcggat cgcggtccag gtggcatcgg ttaattgttc
    13141 ggcgaggttg tcttcgctgt aagtcgcgcg tgggaccttg ctggcgagtg ctgcaagtgt
    13201 gttgttgact gaaatccact tgagttttga gtcctcctcg attgcggctg gcaatgaatc
    13261 aaccggcaac tgcgcggctg ccgcactctc ggcaatgcaa tagacgtaat aagcaaggtc
    13321 ggccatgggc gttatattct ctcacgaacg gcggagtatt tctcccacgc actaaagtgc
    13381 ccgcgcagat tctttgagct tcgaatctcg tgcggttgct gtggcgcgac gaagatctcg
    13441 accgatcggc gccgaaactt agtctgaaac tccttcatca cctgctcctg catctttcgc
    13501 cgcgactggc aaaacttgca gtcattcacc ggtaccaccc cattgatcag taacttactc
    13561 atcggcacct tcaacttttc gagcgacttc gcgaggtcga tcgtctcttc aagactcatg
    13621 cgctccggta tcgccacacc gacaaactcg caacgctccg gatcggtcag cagcgcaatc
    13681 accttcttga tgctctttga cattgctaca agctcttccg ccacctgatt cgcgcgcatc
    13741 acgtctttgt acttcaacaa cagctttata aacgtcctga tccacgagag cgcgacctgc
    13801 ggtaactcca gaaaacgtat caagtgtccg gtcggcgcgg tatcgaggac gagcgtttgg
    13861 tactgttcca gatccaaaag atcactgatg gttccgagcg ccgcgatctc atcgatgccg
    13921 ggcggagtca actcgaccaa ttccctcatt gcttcgcgat cgaatttaat ctccatgcgc
    13981 gaaccaccgg agagcgaagc aaacagctca tcggtccacg tacgatatcg ctccttcaac
    14041 tcttcaaacc acttacccgg atcgatctcc atcgcatcga gatttgcatg accggcaaca
    14101 ccgttctcct gctcgccgat ctcttcatca aaactgtctg acagcgagtg tgccggatca
    14161 gtcgagaaga cgagtatttt ttcttttgga tttgcttgag ccaatgcctg cgcaaatgcc
    14221 gccgcagccg tcgttttccc aacacctcct ttgccaccaa agatcacaat cttccgcggt
    14281 ttcagcggga aggaagactg ggccacggat gacacggatt taaaatccgg attttttatc
    14341 cgtgtcatcc gcggcttcat ttcctccgtc cagatcaact tgccgattct ttttagctcc
    14401 tcaacgccac ggacttcctt cgccattaac ggcacataac gaattctcaa atcgccgaac
    14461 aagctcccga tttctttgat ccacggccgc tgactccgca cgcgcgcacg acaatactta
    14521 caggtaccat gctcctgctc gacacgattc acaatcagat cacgcaccgg gacaccctgc
    14581 tcgctcaaca acgtcaagta tctccccgtc tcacgaacag ccattgcttc gggaatcgtc
    14641 acgagcgtaa aactcgtctg ctcacgatcg tgcagcaact tccgcacagc ctcgatccgc
    14701 cggctcaagt cctgcaaaaa cacgtcaacc tcatccgcca cgggcctgcg acgcgcaaaa
    14761 tgtgcaacga tgtagcgatg cttgtcgccc atcaaatcga gcgccttcac catccggtca
    14821 aacacttccg gcaatcgcaa caaccgcgac gtatgacctg acggcgccgt gtcaaccacg
    14881 atgtgcgcat acttaccctc acggtccaac tcactcagct caaacagcga catcacctca
    14941 tccatgcccg gaagtgacag actcaacaac tcattgatgt cgtcctcgtc aagcagcgtc
    15001 ccgcgctcgg cgatttccgc gagcactttc ccgtgctcag cacgaaacct ctccagcgcc
    15061 agactcgcat ccatctcgta tgcaaacagt cgcgccccgc gatgctgttt cacttcacgg
    15121 agacgattgc cgatttttac gtcaagactg tcagataggg aatgggccgg atcggtggaa
    15181 aacaacagaa tcgattcgtc ttttttgagc ttgttgagga gatagagagc agtcgctgat
    15241 gcggcggtcg tcttgccgac gccacccttg ccaccgaaga aaaggtaacg ggcgttcaac
    15301 ctttgtcgcg gaactcgcct tgatcgtctc cgccaatttc gatcacgaaa ctcgacgatt
    15361 cagccgtatg cgcagtcgct tcatgcatca aatcaagctg ccgcatcttg atcactcgca
    15421 gccgtgcaaa caactccgct tcacgctcgc ggaattcctc gtcggagatc tcctccaact
    15481 cgtagcgcat ctgcaactcg agcagttgct ccttgatcac gctgtcgtcg ttcaactcct
    15541 gatcggccat cttttgaatc tggccgagga tgaacttgaa accgttgacc ggcgccagga
    15601 gtaagtcgtc gattaaaaac attagctggc tactgcttcg gtagcgcctt cgccgcgctc
    15661 gagcttgaga cgaatgtgaa cgaaattata tgggggccag ggaccagtgt acttgaacga
    15721 aagcttgcct tcgtacttcg aagcgatctc tttcactttc tgatcaaact ccgcttcgcg
    15781 atcgcgctcg atcaagaaag ccgcgttcat gatcatgcga tcgccgatcg gtttgttggc
    15841 gcgcgaggca accgcagtgt ctcgcaactg tgaatacacc tcgcgaatat acgcgtccgc
    15901 ctggtctgtc aacgcggtct cgatcaaacg gccaagctgc atgcgtgcga agtaagtcga
    15961 accggtcgta cgcgaaacga tctgctcttt caggtgacga atctcgtcgt ctttctcttc
    16021 cagctcggcg gtgactttgt cgcgatccca gtttaccttc aaaccaaact cgactttgcc
    16081 ttccatcttc gtcagtacgt cgcgcaatgc atcgtaggtg ccgcgaatca gctcgacaat
    16141 atcttcttcc gtacggaaca gcgcaccgaa cgccatcggc agaaccgtaa actcgcgcat
    16201 cactgtctcg ttgacctgct cgtgcgcaaa cacgttctcg cgcgttggat cgtaaacaac
    16261 gagtggcgtg ttggagacga cggccgcgag acctttgtga tgaatagtgt aaacatcatc
    16321 accgcgggcg ccgataccca tcggaccgaa cgagcggggc tcggaagtct cgatgatgca
    16381 gtagacgtat ttgccttcgg tgactgtttc gacagcgggg gtttgttctg ctccactcat
    16441 ttgttttccg aaatagcgcc acgattcggg accgagcggc gccagaaaag atgggatttg
    16501 actacgccgt gaataatact cagttcgtct ctatctgtac aagcattatt ggattaaatc
    16561 aaaacataac atgaaggcgc gatcgaaaac cggatgattg taatgcattg tgctgacgtg
    16621 tccgtccggt aacggacgcg tttgcgggtt ggcatggacg ttgctcgtct tgagactgct
    16681 tttggaaaag gtttgagacg gttttaagaa ggagattcga tcatgctgtg gacaattctg
    16741 gtgattctgt tggtcttgtg gctgattggg ttgctatcac acgtcggcgg cggtctcatt
    16801 cacctgctgc tggtcgttgc ggtaataatc ctggttatca acctcgtgac cggacgccgc
    16861 gctgtttaat cccgtaggat ccgtgttatt ccgcggttat ttaaccgcgg attacacgga
    16921 tcgtttacgg ctttgataaa tcgctattcg ctggtccctg ataaactcta ccgctcgcac
    16981 taaaccgtga accatgacac gggcagtccc acgtctgttc ggtcgcgttc cacgcgacga
    17041 tacatccgag gtgcgggcag accgctgaga gtttgtgtac gttaccgttc tcatcgcgac
    17101 gtacggcaat cttcgaaaca ccttcgcgca tgatcgcgcc cgcgcctggc cgcagctctg
    17161 actctgactt gatatcaccc gccgtcacat aatcagcgta ctgtcctgcg acgttgatgt
    17221 tctccgatgc gtattccggc aacgatttca atcgtaggcg cgaaggactg tacagatctt
    17281 cccacgggtt tttgcgtccc tgaatcaagt cagtcaacaa catgccggca atcgtgccgt
    17341 gcgtcatgcc ctgcccggaa tcgcccgtcg cgatgtaaac gttggcctca tcgagtggat
    17401 tgcggccgat gaaagccaga ccatcgaccg gctccatcac ttgtcccgac cagcgatact
    17461 cgacttcgag tgactgtgga aagcgctcac gcgtccactg ctcgagacgt gcgaaccgct
    17521 cgttgccgtc gtttgcctgt ccggtcttgt gatcttcacc accgacgatc aagacgtcgt
    17581 gatcgttttc acgttgcagc cgcaggtagt gatacggatc acctgtgtcc cagtagagcg
    17641 cacgcgtcac cgagtctttc ggaacgcgca ttccgatcac gtaagtgata tacggcgcct
    17701 gcttcgtatg aatcgcgacg cgatcgttaa ccggagtatt cgtcgcgacg accacgactt
    17761 cgctcttaac tactggacca tgactcgtct ctactatagc ttgcgcgccg ccctcgatct
    17821 tcgtcgcgtg tgtttgtccg aaaatacgac catcctcgcg caggatcgct ttgaccagac
    17881 cgtcgagata cttgagtgga tgaaactgcg cctgtcgtgg gaagcgcagc gccggacctg
    17941 tattgaatga ttcaaccggc gcacgcgcga ctttttcgac tccgttgagt ccggcgcgat
    18001 gcgatgcggc cagctcatcg tcgagtagat caactgagtg ttccggtgcg caaaaaagaa
    18061 atccgtcaag ccactcgaaa tcgcagtcga tcttttctgc cgcgatgatc tggctgatct
    18121 tttcaatcgc cgcgctgtga ctctgcgcag tcagacgcgc gccttccgcg ctatgcagct
    18181 tttcgatctc gagatagcga tcgtcgtaag cgttggtgag atgcgcggtc gtgcgacccg
    18241 tcataccacc gccgctcacg ccgtcgtcca ggacgacaac cgaccggcct tctttggcaa
    18301 gcagatacgc tatcgtcaat ccggcgatgc ccgcaccgat gatacaaacg tcagtgcgaa
    18361 tactttcctt cagccgagat tgagacggcg tatctgccgt agccatccag agggatgtgc
    18421 ttcgttgtga atcgtctttc attggactcc gccgcgtccg cagaaactat ttcgctggcg
    18481 taccgatgtt cacatcgatc cgcttgttgc caccgaacca ccgcccacca aacacgaaca
    18541 aagcggctac cacaaggatc acgaagatta cgagaactgc aatgattccg gtgctcgccg
    18601 tactgctagc attgtctgcc atctgttttc ctcctgatta attgaatcgg gcgctacccg
    18661 cacgttgatc gggcgctgcc cacgcttagc ccgcggtcgt ctcgccgccg agtaacgtca
    18721 aaacttcccc cgtgacgtaa cttgaatcca cttccgacgc gaaataaacg taagcgggcg
    18781 cgatctcttc cggttgcgcc gggcgcttca tcggtgtgtc tgcgccaaac tcagccacgt
    18841 ctttcgcttt cttgtccgcc ggattgagcg gcgtccagac tggaccaggc gcaacacagt
    18901 tcacacgaat gccacgctcg acgagattct gcgcgagcga cttcgtaaac gcgtgaatcg
    18961 ctcctttcgt cgaagcgtag tcaagcaact ctttgctgcc ttcgagaccc gtgatcgatc
    19021 ccgtgttaac gatcgcgctc ccctcctcga gatgctcgag cgccgccttc gtcatgtaga
    19081 aatagccata gatgtttgtc ttgaacgtgc gatcccattg ttcttcggtc acgtcttcaa
    19141 gactttcctg gtgctgttga aacgccgcgt tgttgaccag gatgtccagc ttgccaaact
    19201 tatcaaccgt ctgcttcacc agcttctggc aaactttcgg atcgcgaacg tcgccggtta
    19261 acgcaaacgc ctgttgtccc tcggcttcga tcgcgcgctt cgtttcgtca gcgtcgcgct
    19321 tctctttttt caaaaacgtg aacgcgacgt cagcaccttc gcgcgcaaag agcactgcca
    19381 cagcacgacc gattccggaa tcaccaccag tgatcagcgc taccttctct ttcagttttc
    19441 cggcgccctg atacatcggc gcctggtaat tcggcctggg tttgagtttc gcttcaacgc
    19501 ccggcttctg ctgatgctgt ggcggcatcg gcgacttcgg ctgcttcagt ttcccctttg
    19561 atcccttttt acttgccatg gtccgggcga attgcaaatc acgatccgtg ctggcatcgc
    19621 gattgcgcga aggaagaatg tgtagtgatt taggtgttaa gtgcttcgta actaaacaac
    19681 gcggagttac ggctgtacag gaaagctaca cttcatagga ggttcataaa tggcaaagaa
    19741 accagcgaag agtcatcgtg aattaatcga caccggcaca gacaaacgct ttgtgaggcg
    19801 aaatgacaag gggcaatttg atgaagtggt agatgtggga cactcactcg cgcaggatcg
    19861 caaacaggag gcaaagacca aagttccacc ggggcaaggc gatcgtggcg atcagaagac
    19921 gaagtaattt atttggccgc tgatgaacgc agatgaacgc ggatacaaag aaattcttat
    19981 ccgcgtttat ccgcgtgaat ccgcggcttc ttgtttatcc gttggaaata gtcgttcagc
    20041 attcgagcat gacaaatcaa acacatctgc tccggcagct ccatgttggt cgatctgatc
    20101 aaagcgccac accacgaaca gatgatgtcg tacttcggtt tgagaggatc tatgcgctta
    20161 ctcaatttta accttcctga ctgacctgat acccgaaagg cacaacaaat ctcgttccaa
    20221 tgacaaaact cgaacgtctg cagaacatcg gcatacgtcg actcggcact cccaaacgcg
    20281 gctttcgcta caagccggtc agcggtcgtc tgacgaaagc cgacgtcgac aggattaacg
    20341 atttgaaaat tccgccggcg tggacggagg tgttcatcaa cccggccgca aatggaagag
    20401 tgcaggtgat cggaaaagac actgcgggac gctggcagta tctctatcac gagagccaca
    20461 cacgcacgca ggagcttcgt aaatttcgac gaatcacgaa atttgcgaaa gcgattccgc
    20521 agatgcgcgc gaccgtcgcg cgtcatttac gccagccggg tttgagtcgt gaacgcgtgc
    20581 tcgccgctgt gttgcgcatt ctttcgacgt gctacatgcg accgggcagc gaagtgtatg
    20641 cgagtgagca cggcagttac ggcatcgcca cattgcggcg caaacacgtg accgtcaaag
    20701 gtgagcggat cgcattcgac tttccgggaa agtcaggcgt gcgacagcaa cgcgagctac
    20761 gcgatcgcca ggtcgcgcgc gtgattcgcg agacgatcaa gctgccgggc tacaacgtct
    20821 tcaagtacat aaacggcgac gacaagccgg tgactgtgac gcgacgccat atcaacgagt
    20881 acatcaagga agtgatgggc agtagtttca gtgcgaaaga ctttcgcacg tgggcgggca
    20941 cactcgtttg cgcatgcgcc ctcgcgcgca acggtgtcga gattcccgag aagaagactg
    21001 cgcgaaacaa gcgcatcgtt gaagcgatca aagagactgc ggaagtgctc ggtaacacgc
    21061 cggcagtttg tcgtggctct tacgtgtgtc cggagatcat cagctcgttc gagaagggcc
    21121 aggtgatcga cgcccgtgcc acgaatctcg aagatctgat cggtttccgc gggcgtggtc
    21181 tacacaaagc agaacagtcg ttgctcaagt ttctcaagaa aggtgtcaag tgagattggc
    21241 accgtgattg ctgaatatca agcgggagga ttaatcaatg gaaggtccat taagaggaat
    21301 actggcaggc gcagcagcgt ggaagttcgg cggtggatgt atttccacga tcttgatttt
    21361 cattattgtc ttttgggcgt tgggatacgt tcgttgttag aaacacggat agtacggatc
    21421 gttaagacag gatttgcgcg gatcgatccg tgtaaatcct gtttttaatc cgtgggatcc
    21481 gtgttaccaa ctgacctgca gttcttctgc ttcctgccac accggcaagt tcggacggat
    21541 cgcgcagcgg tacagatgat cgggacggtc caaaaggata ccggctccca tcacgacagt
    21601 gcttattgga tcttccggta cggtcgcccg tagtttcagc tcattttgca ggcgactctt
    21661 caatcccttc aacagcgatc caccgccagt gagaatcatt cccgagtagt agatgtcagc
    21721 gacagcttcc ggttgcgcgt cttcaatcac acgccggatg cccgtcacga tctcattgat
    21781 ggtcggttcc agcgcttcgc gcacttcctc agccgttatc tgcaccgcgc aggcataacc
    21841 atcgctcaac tgcttgccga caagttgaat cttgcgttcg tcggacggaa tgtcatcgtc
    21901 actcagcgca ccgagctcac gcttcagcgc ttcagcagtg tattcaccga tctgcagaca
    21961 gtagttggac cgtacgtaat cgcgaatgct ttcgtccatc gcgtttcccg cagcctgcaa
    22021 actcgtcgat gaaacaactc cacctgatgc gacgatcgcg atgttcgtcg tgccaccacc
    22081 gatatcaacg acgagatgcg cgcgctcatc gtcaaatgca agaccggcgc cgattcccgc
    22141 ggcgagtcct tcatcgacga gatccgtgcg acgaccacca gcatcgtgcg cggcatcgcg
    22201 aaccgagcgt tgctcgacgg cagtcgctga gccaggaatg ccgacgacaa aatgtccgaa
    22261 ccgaattccg tcagtcacct ggccgataaa agatctcagc atcttgcgcg tgacttcgaa
    22321 attgaaaatc gtgccgctgc gaattggccg gtgtacctcg atgccgcgcg gctcacggcc
    22381 gaggagtttg actgctttgt gaccaacgga aacaacctca ccatcgtagc gatcgagtgc
    22441 cacaacggag ggctcgttca aaagaattcc tttgccgggt tgatagatga gtgtgttgac
    22501 tgtgcccaga tctatagcta gctcgttttt gaaaaacttc tcgataaatc tcacggcgac
    22561 ggttcctcct ccaaattggc agggcccacg ggagcgagta atcgctttcc gagcggcaac
    22621 tcgtatgcct tgcaaaagag caattgtctt ctgggggatt gtctttggaa attataaaga
    22681 ttgattaagg cgtggtttcc agtcgcgtat tacaactgtg cggcgaggct ttggaacgta
    22741 cgagttccgc tcgttcattt tgaaataatc cgcgtttatc cgcgtgaatc cgcggttaaa
    22801 actgatctta actgtttcag accctcgagg gtgttgtcgc caaagtcgat accgatcacg
    22861 cgacggccac gcttcacgag agcgcggaaa tccccga
//
LOCUS       DASSFQ010000320        10102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_593491, whole genome shotgun sequence.
ACCESSION   DASSFQ010000320 DASSFQ010000000
VERSION     DASSFQ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593491"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VFH96_11565"
     CDS             complement(<1..1119)
                     /locus_tag="VFH96_11565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6974658.1"
                     /translation="MVTSHKTVIIPGLSRKSLRIAFATPEFITESYFDGGLANYLDRV
                     SRSLVQLGHDVHVVTLSTRNEDAFDHEGVMVHRVMLKPGWNALNYVTRCSLKTTLHWL
                     NFTTQIYRKLKQLHRRKPFDLIQYPNYSSCGLFCIPFLSTVHIVRASSQPGWNKRINQ
                     NLDSALTESLEALQYKLTRNVHAPSFAIQKMLSSDARLIRSPFYVETRDWDTTVYDRF
                     FRGKKYLLYFGRFQLHKGFHTLAQALPQFLKQYPDAYVALVGRDMETSLASSMAAFAR
                     AQCNGFGERLIMVENLRHSFLYPIIKEAHLVVLPSLMDNFPNACLEAMGLGKVVIGTI
                     GTTIDELISDGVNGFLVPPDNPQALADKMIAAWTDQRLA"
     gene            complement(1073..2338)
                     /locus_tag="VFH96_11570"
     CDS             complement(1073..2338)
                     /locus_tag="VFH96_11570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6974659.1"
                     /translation="MKIALCKSHFFGPVSGADEILVSYAISLHNAGHDVDVVLFYKPA
                     ENDRYHSRLRKAGVPVVTVINRSIAFTILRSLRGLLSGFLLVFMLVPSTEKGLRRIWQ
                     VLIDLISRFHYKNCRDYFAQSHYDVLHVFTPDIGATLMIRAGKEAGLPVLYHEMGTPH
                     YLPPLDPHYKRLERVLPLCSEVAALSPTLAQQWSERFPFLPAVSVLPLLTDDAYVLKM
                     PARLNLNATIFGFAARIEEGKGPFVLADALALLRHSRDDVLVRLAGTGPAVQDVKARV
                     RELRLNGSWEFVGAYDGAVGCSAFMRTLDVFVLPSFAEGTSKSVIEAMAHGLPIITTT
                     VGGLPDLLTPDAGILVPPGDTAALAGAMQRLASDPALRERMGQAARERYLKLFAPDAV
                     LSMLVDTYARVAGRNGHESQNGHHPWSIA"
     gene            complement(2343..3245)
                     /locus_tag="VFH96_11575"
     CDS             complement(2343..3245)
                     /locus_tag="VFH96_11575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6974660.1"
                     /translation="MPKISVVIPTYNRARLLPRAIESAQNAGSNLEVIVVDDCSTDDT
                     REVCAQIKDIRYIRLKTNGGLANARNVGIAESSYEFIAFLDDDDLRLPGLLDKQLHAL
                     AADDRIAFCYGQALIGDAHRQLPTGEIYPLRCPQGDIFWDLLENNFVPMPSVLARKSS
                     LISAGKFDTGLKLIADWDMWLRLSEVFLVAAIEEPVAIYRKAVAESGQMCSNAGEICK
                     EALRVQQMALARPRARASESARRRDVRRKFRDRAYHILMTEASKSIHEGKPKSARSNI
                     LDAFRLRPFRTVASGGLFWLLSHR"
     gene            complement(3338..3706)
                     /locus_tag="VFH96_11580"
     CDS             complement(3338..3706)
                     /locus_tag="VFH96_11580"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6974661.1"
                     /translation="MATLVNSQRTNVNKDNWLGFVGDVVNFTGEVCFKSMLRIDGHFS
                     GNVTSEDGTLIVSTGAQVTKAVINVAVAKINGTVEGDIRATKELVLGRTANVKGQVAS
                     PALVVEEGAQLNGSCRRLIN"
     gene            3851..4825
                     /locus_tag="VFH96_11585"
     CDS             3851..4825
                     /locus_tag="VFH96_11585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6974662.1"
                     /translation="MKFDLHTHFYTEAFFQTIRDLPSEFSFGASSTGQTIITHRGARF
                     FGVTPAMTDVSKRIEDMDRVGIDVEVVSLSTPNVFFTDAAHQPEVAKMMNDSYADLIA
                     RHPKRFKGFASIPMDAPDAALAELHRAIDTLKLNGVILLSNIGGKPLTSPEYRPFFEE
                     ANRMKLCIFLHPMIPANSDAFREYVLGPIIGFPFDTSLAVARMCYDGMFEELPDIRWI
                     IGHLGGAIPYLMERMDNGFRDFADCRVKIDKLPSVYLKRLYYDTVSFSPYTLKMVRDM
                     VGADHMVMGSDYPHLLGSIDKAVTSILDLEGDHDQIFSGTALSILNNI"
     gene            complement(4878..6965)
                     /locus_tag="VFH96_11590"
     CDS             complement(4878..6965)
                     /locus_tag="VFH96_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6974663.1"
                     /translation="MKFNAIFLLVALCCVVANGQETEVVRTNTELVQTAITVLDKKGH
                     FVEGLQRDQFQLIVDGKPRPVAFFERVAAGSPRELEIAAPGNENINTKTSVPRVPGRT
                     IVFFIDDLHLSPDSMNRSKMMLQHFLDREMSSKDNIAILTASGQVGFLEQFTNNRAVL
                     DAAMSRLIPRMYDAEGYSYGNSTKMTEYMAFNIDTSKTDRKVMDFYIEECMKGSNTFK
                     KAQQVAALIRASCETQVKSSARAVLMQAAQITQNTYISLESAMRSLARAPGRKLAFFV
                     SDGFLLDAGPRAANVRDKLDHVIDAAQRAGVVVYTIHAAGLVNSTFLDPGNKRPMDIQ
                     GRLDIASTGELAANQDALSAIASDTGGRALRGMNFFDKWVSTVLDETSNYYVVAWRPE
                     NEDEKLPKFRNVKITVVSRPELTVRAPKGYVTGPQPTATTTTAKNDKPKTPDAELRDA
                     LGDFYPTSGLPTLLSLTYLNTPANGLVVTSSIEILGTGAQGATIKLAGVVLNDKGKIA
                     SSFKNQLNIDAPKNGGSDSIFYNHHTPLAPGIYQFRVAARDEKTGRVGSAIQWIVIPD
                     LTKSQLTLSSVLLGGQVLEDKSTPHVQLSVDHTFPRMSQMGYWVFVYNAKRDASGKPQ
                     ITVQTQVLRDGQAVLSSMRKVSNAGPDLERIPFGEELALNTLAPGKYDLKVTVTDGIT
                     GTSTSQLADFIVK"
     gene            complement(7035..>10102)
                     /locus_tag="VFH96_11595"
     CDS             complement(7035..>10102)
                     /locus_tag="VFH96_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6974664.1"
                     /translation="PGADQVQGWTPDGKSVMFASGRASLAPNAAPRFWTVPVEGGVEQ
                     PMPLPRGYQGKISPDGRRIAYRMNNSWDEERRNYRGGQNRPIWIVDLKTYDLVSPPWT
                     DSKDVNPVWVDDSVFFISDRDGVANVWEYQTKTKKLAQITKFTDFDVKSMDSGAGTVV
                     FEQAGYVHELDPKSGKEKVVNIVAAGDFPWMMANWEDVSNRMTNMAISPTGKRVVVES
                     RGEIFTIPADKGDVRNLTNSSSSAERDPAWSPDGKFVSYFSDKSGEYKVVIEAQDGLT
                     PPREIALAKPTHYYTPSWSPDSKKLLFTDTNLKVWVLDVASGQAKIVGEDPWMVPQRT
                     LNPVWSPDSKWVAYASRLRSLYHAIFVSNVETGETKQITDGLADAVWPAWDASGKYLW
                     FLASTDFGLRSQWLDMTSYDHNPTFGLYFAVLKKSDASPLLPESDEDRGVGTGTRRLQ
                     PGACAGEGGPAEGAEGGGGGQRGPRAPVTVQVDFDGLAQRIVAVPGVPERQYSDLQAG
                     VDGQVFYLEAGRAGGQGGPAGGGNELQRYRLCDRRASTFVSNVAAYEISADRRKLVYR
                     AAAGGGGQGGGPGGGIGANAPAPQLFLVDADRTPPQAGAGRLNYSLRMYLDPKEEFKQ
                     IFNEGWRNQRDYLYVPNMHGADWPKMREMYGQLLPYVRHRADLNYLMDMMGAEIAIGH
                     SYVRGGDMPTVPQPVAGLLGADFAIENGRYKVTRIYDNESWNPDLRSPLATPGANVNT
                     GDYILAINGIELIAPDNIYRLLDGTANRQTTLTVNTTPSMQGARTLTVVPVANEQALR
                     TRAWVESNRRLVEKLSDGQLAYVYLPNTGQPGYTSFNRYYFSQQDKKGVVVDERFNGG
                     GSAADYIIEVLARDFDGYFNNVAGERYPFTSPSAGIWGPKVMIINEMAGSGGDLMPYM
                     FKRRKIGPLIGKRTWGGLVHTADTPLFIDGGSMIAPRGGFFTRDGKWAVENEGVGPDI
                     DVENWPKDVVAGHDPQLERAVQEAMRLLKERPVERMPKEPAPPTWGKRP"
BASE COUNT         2166 a         2875 c         2786 g         2275 t
ORIGIN      
        1 tgccaaacgc tgatcggtcc acgctgcgat catcttgtct gccaacgcct ggggattgtc
       61 aggtgggacg agaaatccgt tcacgccatc gctaatcaat tcgtcaattg tggtgccgat
      121 cgtgccgatt accactttgc cgagtcccat cgcttccaaa catgcgttcg ggaagttgtc
      181 catcaaagac ggcaagacga cgaggtgcgc ttccttgatg atcggataca agaaactgtg
      241 ccgcaggttc tccaccatta ttagtcgctc gccaaatccg ttgcactggg cgcgcgcaaa
      301 agccgccatc gacgacgcca gactggtttc catgtctctg cccacgagcg caacatacgc
      361 atccgggtat tgcttcagaa actgcggcaa agcctgtgct agtgtgtgaa agcctttgtg
      421 caactgaaag cgaccgaaat agagcaaata ttttttgcct ctaaaaaatc gatcgtagac
      481 cgtggtatcc cagtcgcggg tctcgacgta aaaaggtgag cgaatcaatc gcgcatcact
      541 cgagagcatt ttctgaatcg caaagctggg cgcatgaacg ttgcgcgtga gcttgtattg
      601 cagcgcttcg aggctctcgg tcagagccga gtcgagattc tggttgatac gtttgttcca
      661 gcccggttgc gatgatgctc tgacgatgtg caccgtgctc aggaagggaa tacagaacag
      721 accgcacgaa gagtagtttg gatactggat caaatcgaac ggcttacgac gatgaagttg
      781 tttgagtttg cgatagatct gcgtggtgaa attgagccag tgcaaagtcg ttttcaacga
      841 acagcgcgtg acgtagttga gcgcgttcca gcccggcttg agcatcacgc ggtggaccat
      901 caccccttca tgatcgaatg catcttcgtt tctggtggag agcgttacga cgtgaacatc
      961 gtgaccgagc tgcacgagcg acctggaaac gcgatcgaga taattggcta atccgccgtc
     1021 gaagtaactt tcagtaatga attccggtgt ggcgaaggcg atacgcaagg atttacgcga
     1081 tagaccaggg atgatgaccg ttttgtgact cgtgaccatt gcgacccgcg acccgagcgt
     1141 aagtgtcgac cagcatcgac aacactgcgt ccggcgcaaa caatttcaga taacgctcgc
     1201 gcgcggcctg gcccatgcgt tcccgaagcg ctggatcaga cgcgagacgt tgcatggctc
     1261 ccgccaatgc agcggtgtcg ccgggcggaa cgagaattcc cgcatcagga gttaacagat
     1321 caggcaagcc gccaacggtg gtggtgataa tcggtagtcc gtgcgccatc gcttcgataa
     1381 cactcttcga ggttccttca gcaaacgaag gcaacacgaa cacatcgagc gtgcgcataa
     1441 acgcgctgca cccaaccgcg ccatcatacg ctccgacaaa ctcccacgat ccattcaatc
     1501 gtaattcgcg cacgcgtgct ttcacgtcct gcactgcggg accagtgccc gctaaacgaa
     1561 cgagcacatc gtcgcggcta tgtcgtaaca gcgcgagcgc gtcagccagc acaaatggtc
     1621 ctttgccctc ctcgatccgt gccgcgaatc cgaagattgt agcgttcaag ttcaacctgg
     1681 ccggcatctt caatacatac gcgtcgtcag taagcaaagg caacacggaa acagccggca
     1741 gaaaaggaaa acgttcggac cactgctgcg cgagtgtcgg cgaaagcgcc gcgacttccg
     1801 aacagagcgg caacactctc tcgagtcgtt tgtagtgtgg atcgagcggc ggcaggtaat
     1861 gcggcgttcc catctcgtga tacagcaccg gaagtcctgc ttctttaccg gcgcgaatca
     1921 tgagcgtggc gccgatgtcg ggcgtgaaca cgtgcagcac gtcgtaatgc gattgcgcga
     1981 agtaatcgcg gcagttttta tagtgaaaac gcgagatgag atcgatgagc acttgccaga
     2041 ttcttcgtaa acctttttca gtgctcggaa cgagcatgaa gacgagcaaa aaaccggaca
     2101 acaaaccgcg cagactacgc agaatcgtga atgcgatcga gcgattgatg acggtgacaa
     2161 caggaacgcc ggctttgcga aggcggctat ggtagcgatc attctcagcg ggtttgtaga
     2221 acaggactac gtcaacatcg tgtccggcgt tgtgcaggct gatggcgtag ctgacgagta
     2281 tctcgtccgc gccgctcact ggtccaaaga agtgactctt gcaaagagca atcttcataa
     2341 acttaacggt gactcagcag ccaaaagagc ccgccgctcg ccacagtgcg aaacgggcgc
     2401 agtcgaaaag catcgagaat gttggatctg gctgattttg gtttcccttc gtggatcgac
     2461 ttgctggctt cagtcattaa tatgtggtac gcgcggtcgc ggaatttccg tcgcacgtca
     2521 cggcgcctgg cgctctccga cgcacgcgcg cgcggacgag caagcgccat ctgctgcacg
     2581 cgcaaagcct ctttacaaat ttctccggca ttggaacaca tctgcccgga ttcggcgact
     2641 gccttacgat aaatcgcgac tggctcctca atcgccgcca ccaaaaaaac ctcactcaaa
     2701 cgcagccaca tgtcccagtc tgcgattagc ttcagccccg tatcgaattt accggcgcta
     2761 attaaactag acttacgtgc gagcacggac ggcattggca caaagttatt ttcgagaagg
     2821 tcccagaaga tgtcgccctg cggacagcgc agcggataga tctcaccggt cgggagctgc
     2881 cggtgcgcgt cgccgatcaa cgcctggccg taacagaagg cgattcgatc gtcagcggcg
     2941 agcgcatgca attgtttgtc gagcaagcct ggcaggcgca ggtcgtcgtc atcgagaaaa
     3001 gcgatgaact catacgagct ctcagcgatg ccaacgttgc gtgcattcgc gagaccgccg
     3061 tttgtcttca atcggatata gcgaatgtct ttgatttggg cgcacacttc gcgcgtgtcg
     3121 tcagtcgaac agtcatcgac aacgatcact tcgagatttg aaccggcatt ttgcgcgctc
     3181 tcgattgcgc gcggcagcag ccgggcacgg ttataagtag ggatgacgac gcttattttc
     3241 ggcacactgt gtgtgaggat gtatattagc cacaaagagg cacaaaaagc acaaaaattt
     3301 tcagcagatg attttgtgcc ttttgtgcct tttgtggcta attgataagt cgccggcaac
     3361 tgccgttgag ttgtgcgcct tcttcaacga ctaacgcggg tgaggcaact tgccctttga
     3421 cgtttgcggt gcggccgagc acgagttctt tcgtcgcgcg aatgtcgccc tcaacggttc
     3481 cattgatctt cgcgaccgcg acgttgatga cggcttttgt aacttgcgcg ccggtgctga
     3541 cgatgagcgt tccgtcttct gacgtcacat ttccggagaa gtgcccatcg atacgcagca
     3601 tcgatttgaa gcagacttcg ccggtaaaat tcacgacgtc gccgacaaag ccgagccaat
     3661 tatctttatt gacattggtc cgttgactat taacgagcgt tgccacagtg tgcctctgga
     3721 gaatcgggga aggaagaaaa ttacctggct gtcgacaata tatcatctgc gttgttgtgc
     3781 ggagtgttac gattagattg ccgttctgag gcatccacgt gaagacgaat caatatctac
     3841 gatcaaagtg atgaagtttg acctgcatac gcatttctac actgaagcat tctttcaaac
     3901 cattcgcgat cttccttccg agttttcttt cggcgccagc tccaccgggc agacgataat
     3961 tacgcatcgc ggcgcgcgat tttttggcgt cactccggcg atgactgacg tttcgaaacg
     4021 catcgaggat atggatcgcg tcggcataga cgttgaagtc gtttcactat caacgccgaa
     4081 cgtctttttt actgacgccg cgcaccagcc cgaagtcgcg aagatgatga acgactccta
     4141 cgcggatctc atcgcgcgtc atccgaaacg gttcaaaggc ttcgcgtcga ttccgatgga
     4201 cgcgcctgac gccgcgctcg cagagctgca ccgcgcgatc gatacactga aactcaatgg
     4261 cgtcattttg ttgagcaaca tcggtggcaa gccgctgacg tcgcccgagt accggccgtt
     4321 tttcgaagaa gcgaaccgga tgaagctgtg tatttttctg catccgatga ttccggccaa
     4381 ttcggatgcg tttcgggagt atgtgctggg gccgatcatc gggtttccgt ttgatacaag
     4441 tctggcagtg gcgcggatgt gttacgacgg aatgtttgaa gagctgccgg acatacgttg
     4501 gatcatcggg catctcggcg gcgcgatacc atacctgatg gagcggatgg acaacggctt
     4561 tcgagatttt gctgattgcc gggtgaagat cgataagctg ccgagcgtct atttgaagcg
     4621 cttgtattac gacaccgtgt cgttcagtcc gtacacgtta aagatggtgc gcgacatggt
     4681 tggcgcggat catatggtga tgggtagtga ctatccgcac ttgctgggat cgattgataa
     4741 ggcggtcacg tcgattctgg atcttgaagg cgatcacgac cagatctttt ccgggacggc
     4801 gctcagcatc ctgaataata tttagccacg gatgacacgg atttaaaatc cggattttta
     4861 tccgtgttat ccgtggctca tttcacgatg aagtccgcta gctgagaggt actggtcccg
     4921 gttatgccat ctgttacggt tactttgaga tcatatttcc caggcgcgag cgtattcaga
     4981 gccaattctt ccccaaatgg aatccgttcc agatcgggac cggcattact cactttccgc
     5041 atcgagctca agacggcctg accatcacgg agcacctgtg tctgcacggt gatctgcggc
     5101 tttccgctgg catcgcgctt agcgttgtag acaaacaccc agtaacccat ttgcgacatg
     5161 cgtgggaaag tgtgatccac gctcaactgc acgtgcggag tgctcttgtc ttcgagcacc
     5221 tgtccgccca gcaatacgct gctcaacgtt agctggctct tcgtcagatc gggaatcacg
     5281 atccactgga tcgcgctgcc gacgcgtcct gttttttcat cacgcgccgc cacccgaaac
     5341 tggtagatgc cgggcgcgag tggtgtgtga tgattgtaga agatcgaatc cgagccgccg
     5401 ttcttcggcg catcgatgtt gagttggttc ttgaagctcg aggcgatctt gcctttgtcg
     5461 ttcagtacaa cgccggcgag tttgatcgtt gccccctgcg cgcccgtgcc taagatctcg
     5521 atcgatgacg tcacgaccag accattcgcg ggagtattca aatacgtgag cgacaaaagc
     5581 gtcggcaagc cactcgtcgg ataaaaatca ccgagcgcgt cacgcagctc ggcgtccgga
     5641 gttttgggtt tgtcgttctt ggcagtggtc gtcgtcgcgg ttggttgtgg accagtgaca
     5701 taacctttcg gcgcacgcac cgttagctcc ggccggctca caactgtgat cttcacgttt
     5761 cgaaacttcg gcagtttctc gtcctcgttc tcgggtcgcc acgccacgac gtaatagttc
     5821 gaagtctcgt cgagcacggt cgacacccat ttgtcgaaga agttcatgcc gcgaagcgcg
     5881 cgcccgccgg tgtcactcgc gatggcgctg agtgcgtcct ggttagcggc gagctcgccg
     5941 gtagacgcga tatcgagccg gccttgtata tccatcggac gcttgtttcc gggatcgagg
     6001 aaagtgctgt tgacgagtcc ggcggcgtga atcgtgtaaa caacgacgcc tgcgcgttgg
     6061 gcggcgtcga tgacgtgatc cagtttgtcg cgcacattcg cggcgcgtgg accagcgtcg
     6121 agcagaaaac catcggagac gaagaaagcg agtttgcgtc ccggcgcgcg cgcgagcgag
     6181 cgcatggcgg attcgagtga gatgtaagtg ttctgcgtga tctgtgctgc ctgcatcagc
     6241 acggcgcggg cgctactttt gacttgcgtt tcacacgagg cgcgaatgag cgctgcgact
     6301 tgctgtgcct ttttgaaggt atttgagcct ttcatgcact cttcgatgta gaaatccatg
     6361 accttacgat cggtcttgga cgtatcgata ttgaatgcca tgtactccgt catttttgtt
     6421 gaattaccgt agctgtaacc ctcggcgtcg tacatgcgcg gaatgaggcg cgacatcgcg
     6481 gcatcgagga cggcgcggtt gttggtgaac tgttcgagaa agccgacttg tccactcgcg
     6541 gtgagaatcg cgatgttgtc tttcgagctc atttcgcgat cgagaaaatg ctggagcatc
     6601 attttcgagc ggttcatgct gtcaggcgag aggtgtagat cgtcgatgaa gaagacgatc
     6661 gtgcggcccg ggactcgcgg cacggatgtt ttagtgttga tgttctcgtt accaggagcc
     6721 gcaatctcta attcacgtgg actacctgct gcaactcgtt cgaaaaacgc caccggccgc
     6781 ggcttgccat cgactatcaa ctgaaattga tcgcgctgca gtccttcgac gaagtggccc
     6841 ttcttgtcga gtacggtgat cgcggtttga actaattccg tgttggtgcg gacgacctcg
     6901 gtctcctggc cgttagccac cacacaacag agcgcgacga gtaaaaagat tgcattaaac
     6961 ttcattgctt cccccggtca attttgccac ggattacacg gatgaaatcc ggattttatc
     7021 cgtgttatcc gtggttacgg tcttttcccc catgtcggtg gcgcgggttc ttttggcatg
     7081 cgttccacgg ggcgctcctt gagtagtcgc attgcttcct gcacggcgcg ttcgagttgt
     7141 ggatcgtggc cggcgacaac atctttgggc cagttctcaa cgtcgatatc ggggccgacg
     7201 ccttcgttct caaccgccca tttgccgtcg cgcgtgaaga aaccaccgcg gggtgcgatc
     7261 atcgatccac catcgatgaa tagcggtgtg tcggcggtat gaacaagtcc gccccacgtg
     7321 cgcttgccga tcaatggacc aatcttgcgg cgcttgaaca tatacggcat caggtcgccg
     7381 cccgaaccgg ccatttcgtt gatgatcatt accttcggtc cccagatgcc cgccgatgga
     7441 ctcgtaaatg gataacgctc accggcgacg ttgttgaagt aaccatcaaa gtcacgcgcg
     7501 agcacctcga ttatgtaatc tgccgccgag ccgccgccgt taaatcgctc gtcaacgacc
     7561 acgcctttct tatcctgttg cgagaagtag taacgattga aactcgtgta gcccggctgt
     7621 ccggtgttcg gcaggtagac atacgcgagc tgcccatccg aaagcttctc aaccaggcga
     7681 cgattcgact cgacccacgc gcgcgtgcgc agcgcttgct cgttcgcaac cggcacgact
     7741 gtcaacgtac gcgcgccctg catcgacggc gtagtgttta cggttagtgt ggtttggcga
     7801 ttcgcagtgc cgtcgagtaa gcggtaaatg ttatcgggcg cgatcaattc gatgccgttg
     7861 atggcaagaa tgtagtcacc ggtgttgacg tttgcgccgg gtgttgcgag tggcgaacgc
     7921 agatcgggat tccagctctc gttgtcgtaa atgcgtgtga ccttgtaccg accgttctcg
     7981 atggcgaagt cagcaccgag tagacctgcc acaggctgcg gcacggtcgg catatcgccg
     8041 ccacgcacgt atgagtgacc gatcgcgatc tccgcgccca tcatgtccat caggtaattg
     8101 agatccgcgc ggtgccgcac gtaaggcagc aactgcccgt acatctcgcg catcttcggc
     8161 cagtcagcgc catgcatgtt cggcacgtag agatagtcgc gctggttgcg ccaaccctcg
     8221 ttgaatatct gtttgaactc ttccttagga tcgagataca tccttaacga gtagttcaaa
     8281 cgtccggcgc ctgcctgtgg cggtgttcga tccgcatcga cgagaaacaa ctgcggcgca
     8341 ggcgcatttg caccaatgcc accgcctgga ccaccgccct gaccaccgcc accagcagcc
     8401 gcgcgataaa cgagtttgcg acgatccgcg ctgatctcgt acgccgcgac attgctgaca
     8461 aaggtcgacg cacgacgatc gcagagacga tagcgctgca actcgttacc accacctgcc
     8521 ggaccacctt ggccgccggc gcgacccgcc tcgagataaa acacctggcc atcgacgccg
     8581 gcctgaagat cggaatactg gcgctccggc acacccggca ccgcgacaat acgttgcgct
     8641 aaaccgtcga aatcgacctg cacagtcacg ggcgcgcgtg gtccacgctg gcctcctcct
     8701 ccgccttcag caccttcagc cggcccacct tcaccggcac acgcaccggg ctgcagtctg
     8761 cgcgtgcccg taccaacacc acgatcttca tcactttccg gcagcaacgg gctcgcatca
     8821 ctctttttca ataccgcgaa atacagccca aacgtcggat tgtgatcgta cgaagtcatg
     8881 tcgagccact gtgagcgcag tccgaaatct gtcgacgcga gaaaccagag atactttcca
     8941 ctcgcatccc acgccggcca aacagcgtcc gcgagaccgt cagtgatctg cttcgtctca
     9001 cccgtctcaa cgttgctgac gaagatcgcg tgatacagcg aacgcaaacg acttgcatac
     9061 gcgacccact tcgaatccgg actccatacc ggattcagcg tgcgctgcgg caccatccac
     9121 ggatcctcgc ccacgatctt cgcctgcccg ctcgcaacgt cgagcaccca cactttcaaa
     9181 ttcgtgtcag tgaaaaggag cttcttcgaa tcgggcgacc atgacggtgt gtagtaatgc
     9241 gtcggctttg cgagcgcgat ctcgcgtggc ggcgtgagcc catcctgcgc ttcgatcacg
     9301 actttgtact cgcctgactt gtcgctgaag tacgaaacaa acttgccgtc aggcgaccac
     9361 gccggatcgc gctcggccga gctactcgag ttcgtcagat tgcgcacgtc gcctttgtca
     9421 gccggtatcg tgaagatctc gccgcgcgat tccaccacca cgcgtttgcc cgtcggcgaa
     9481 atggccatgt tcgtcatacg gttcgacacg tcttcccagt tcgccatcat ccatggaaaa
     9541 tcacccgccg cgacgatgtt gacgaccttc tccttgccac tcttcggatc gagctcgtgc
     9601 acgtagcctg cctgttcgaa cacgaccgtg cccgcgcccg agtccatcga cttcacatcg
     9661 aagtcagtaa acttcgtgat ctgcgccagc ttcttcgtct tcgtttgata ctcccaaaca
     9721 ttcgcgacgc catcgcgatc ggagatgaag aaaacggaat catcgaccca caccggattc
     9781 acgtcttttg aatccgtcca tggcggcgaa acgagatcgt acgttttgag atcgacgatc
     9841 cagatcggac gattttgtcc gccgcgataa ttgcgacgtt cttcgtccca cgagttgttc
     9901 atgcgatacg cgatgcggcg tccatcgggc gaaatctttc cctgatagcc gcgcggcagc
     9961 ggcatcggct gctcgacgcc gccctcgacc ggcacggtcc agaaccgcgg cgccgcgttc
    10021 ggcgcgaggc tcgcgcggcc cgacgcgaac atcaccgact tgccatcggg cgtccagccc
    10081 tggacctgat cggcgccggg at
//
LOCUS       DASSFQ010000321         9121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1010
            k141_1482544, whole genome shotgun sequence.
ACCESSION   DASSFQ010000321 DASSFQ010000000
VERSION     DASSFQ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963176
            Sequence Read Archive: SRR25158410
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:00:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,327
            CDSs (total)                      :: 2,313
            Genes (coding)                    :: 2,306
            CDSs (with protein)               :: 2,306
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 12
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482544"
                     /isolate="SMAG_U1010"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39666667 N 109.5719444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..1395
                     /locus_tag="VFH96_11600"
     CDS             412..1395
                     /locus_tag="VFH96_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET6974665.1"
                     /translation="MPDYRFLQFDVFTDRAFCGNPLAVFTDAEGIPDDQMGKIAREMN
                     LSETVFVLKSESDQALRHLRIFTPAREIPFAGHPIVGTWAALAGQGVVPLPDGGNGWQ
                     RIFHEVGIGILPVDIEFKDGQPVQVVMTQGKFEIINEIDDPHEQAEVARALGLAREDL
                     DESLPIQVITTGLSCLAVPIRSLADLRDIRVDAALLADIYTRYGGAGCHAFTRETLEV
                     GAARAHARFFAPADNIPEDPATGSACGALGGYLIHHGALSLEPEDGRYKFVIEQGDFI
                     NRPSRINLNVKGQTGNVEEVKVGGSAVLVARGEVITEPSAVAPGQPHQSGT"
     gene            1396..2418
                     /locus_tag="VFH96_11605"
     CDS             1396..2418
                     /locus_tag="VFH96_11605"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6974666.1"
                     /translation="MNVPGATALGSVHMLDLMIIGAGPAGLSAAEAAAQAGLDYIVIE
                     KGTIANTIRQYPVGRAMFSTPNEVEMHAGTLRHVSEKPTREEMLSHYIHFVLDLDLKI
                     NTGEEVVSVNGNINEGFVVKTDRGEYEARRVLFAIGAMEHPRRLNVPGEELPKVHHLF
                     VEPYAYVRKNALVVGGGNSAAEAALFLAEEGARTTMAIWREDWENRDPKAGAMKHWVR
                     TPLEAQVAAGRLNVVLYKNVDEIHEDDVTLTTETGESLTIPNDVVFVLVGSDADLTLL
                     KQLGVQTQPGKLAEVPVYDPETFETNVRGVYVAGHFTHARHIKAAIDVPRQIVPLIAH
                     ELKSSR"
     gene            2509..4287
                     /locus_tag="VFH96_11610"
     CDS             2509..4287
                     /locus_tag="VFH96_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6974667.1"
                     /translation="MTRLALLISLLICLASPINAQKKTVSSLCTRDNALDTAKQQILI
                     TRTFDNPVHRITVLVRAADLLWPHDQEKSLAAFMEAFDFAVQNFKEKGDQIQRTSSSR
                     FAAVIPVPDQRFVVLSALAKRDPARARKLSEQMLRDDVSEADKPATDDQSKRSSAGKL
                     LTMAFSLAASDPPSAVNFARQSFRYPATMQLPIFLYELAKSSPQLADQFYVEALNAYG
                     AAPMDQFLYLSSYPFANKRDAGEMPGYTFYVIPEGFEPNRSLQRQFVRTLLARIDGAM
                     ETPVQEAQRLSDHAQMWLALNRLEKQVQSNLPEFTDAFAQSKDKLFALLTPNTQRDVN
                     QLITSDNTPARSFDEQVEAAQKQTNVGRRDQGLSFAVTGSSKDEPVDKVLSVIDKISE
                     ASIRDALLNWFYYFRSQAFIKEKNFSEARKFASKVTELDQRAYLYTRIAEESLKAAED
                     QTEVREMLNEVASTINKAPKTIVSARALLALAYLYAKVDMNRGVEELGNAVTTINTLE
                     SPDFSRQFVMIKIEGKTFGSFASFATPGFNPENAFREMGKLDFDGSLAQATNFSDKSL
                     RALTTLAVIEPCLATKPIRAPKTKAP"
     gene            complement(4315..5730)
                     /locus_tag="VFH96_11615"
     CDS             complement(4315..5730)
                     /locus_tag="VFH96_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HET6974668.1"
                     /translation="MQPTPSASYSLTLRVRLSSRAGSLGELTTAIGRAGGDIGAIDIV
                     TVGNNYIIRDITVSSVSSKHGEQIVETVKDVDGVELLQVSDPTFLMHLGGKIEVVSKV
                     PLKTRADLSMAYTPGVARICEAIAKDPEKVFTLTIKKNTVAIVTDGTAVLGLGDIGPA
                     AALPVMEGKAMLFKEFAGVDAFPICLNTKDPDEIVRTVKAISTVFGGINLEDISAPRC
                     FQIEERLREELDIPVFHDDQHGTAVVVLAALINALKLVGKQMSDVKVIVNGVGAAGVA
                     CTKIIMAAGVKNIVGCDQTGALYRGRRENMNWMKDWYAQNTNPNDEKGTVHDVIKGAD
                     VFFGLSVPGVIGVEDVKNMAEKPIVFAMANPVPEIMPEDAAPYVAVMATGRSDYPNQI
                     NNVLSFPGIFRGALACRAARINEEMKLAASYAIAAIISDSELHPEYIVPSVFDKRVAE
                     AVAREVEAAAQQTGVARREIS"
     gene            complement(5749..6633)
                     /locus_tag="VFH96_11620"
     CDS             complement(5749..6633)
                     /locus_tag="VFH96_11620"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HET6974669.1"
                     /translation="MPDAKRKQKSEVSIIGTGRLGTTLALALSGSGYTIRSLVSRGGQ
                     SARKAARLLDAAVQVLAAKQLRTLIDADLFLITVPDDQIPRVADELSNVRFNADQKKR
                     RTALHTSGALSSEVLAPLREKGWHTGSIHPLMSVSAVHDAHLEGAFWSVEGDPSALRL
                     GKQIVRVLGGKSFSIRTEDKPLYHAAAVMVSGNVVALFDVALEMLGECGLDRKTARAV
                     LLPLIASTVRSLQTKSPEESLTGTFSRGDVETVKRHLAALKKKELAEALDLYRLLGQR
                     SLKLTKEHPQITQILKSV"
     gene            6812..8011
                     /locus_tag="VFH96_11625"
     CDS             6812..8011
                     /locus_tag="VFH96_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6974670.1"
                     /translation="MNNFSRATSAFVLTASLLFSSVAAVAQTTATKPADNAQTTSQTQ
                     SDQKTQTDAKAEAQKATPAPVAVKTSNKPLSTNENPEMIGKRNINTGIIAKMSGSTEK
                     EVRQGREAAAEVDRQAKFVDDPLITEYVNRVGQNIVLHSDAKVPFTIKVIDSDEVNAF
                     ALPGGFFYVNKGLILAADNEAELAGVMAHEIAHVAARHAVENQTKASLLEYAALGGSI
                     FLGGIPGMIYQNTAGIGLLGIFMKFSRSAEEEADKLGVQYMYAAGYDPGAMATMFEKL
                     EAKNKKKPGFVARAFSTHPAPPDRRASALALAARFPEHEEYVISSSEFQRVKGRLLRL
                     SNARASTAGAIQAGDDSGAPGRPTLKRRQPTPEETGTTPDGEQQQKDAPPKLKKDPSA
                     TKTPDKP"
     gene            8227..8679
                     /gene="rplM"
                     /locus_tag="VFH96_11630"
     CDS             8227..8679
                     /gene="rplM"
                     /locus_tag="VFH96_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET6974671.1"
                     /translation="MSTYFPSGKELAGRRKWYVVDASGQTVGHLASEVASILSGKRSP
                     EWTPFMDMGDHVVVINASKAVLKGNKVEQKLYRHHTRYPGGLRTTTAKEMIAKRPERV
                     IELAIKGMLPKTKLGKAMAKKLKIYADAEHPHSAQRPQAYDLTHNTNS"
     gene            8710..9102
                     /gene="rpsI"
                     /locus_tag="VFH96_11635"
     CDS             8710..9102
                     /gene="rpsI"
                     /locus_tag="VFH96_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET6974672.1"
                     /translation="MAEIQYYGTGRRKSATARVYLRPGGGEVKVNHKPFDRYFPNETL
                     RMIIRQPLQLTETTNKFDLVINVAGGGPAGQAGAIRHGITRALLEFNADLRPALKHAG
                     LVTRDPRIKERKKYGQKGARKRFQFSKR"
BASE COUNT         2110 a         2625 c         2490 g         1896 t
ORIGIN      
        1 tgggagattt taggcccgcc gcgcgcatat gccaagcgat tttgagcgcg gggattttgg
       61 cgagcgcgcg acgaaaactc gtgccagcgc gcgaagcgcg cgacgcaaaa agcacaggca
      121 agcgcgcgaa gcgcgcgaca gcataaagcc tggggcgcaa gccccaggat ccgaatcaaa
      181 aatacgattg agcccgcgaa gcggccggca gcgcatcaat gcttttgtgc cgtcgcccac
      241 ttcgcgggct cgttcgcttt ttataatcgt tatcctgggg cttgcgcccc aggctttatg
      301 cttacgcctg ctatcgcagg ctaagaaatc attcgatttc cacttttgtg caaagcgtct
      361 cttggcgcaa cctttgcgcg ggggcgtgaa ctgttttaac ttctactcac catgccagac
      421 tatcgctttc tccaattcga cgtattcacc gatcgcgcct tctgtggcaa tccactcgca
      481 gtcttcaccg atgctgaagg aatacctgac gatcagatgg gaaagatcgc gcgtgagatg
      541 aatctctctg aaaccgtctt cgtgctcaaa tcagaatcgg accaggcact gcgacacctg
      601 cgcatcttca cgcccgcgcg tgagatcccg tttgccggac atccgatcgt cggcacctgg
      661 gctgcgctcg ccggccaggg tgtggtcccg cttcccgacg gcggcaatgg ttggcagcgg
      721 atctttcacg aagtcgggat cggaatcctg ccggtcgata tcgagtttaa agacggacaa
      781 ccggtgcagg tcgtcatgac tcagggcaaa ttcgaaatca tcaacgagat cgacgatcca
      841 cacgagcaag ccgaggtagc gcgtgcgctt ggtctcgccc gcgaagatct cgatgagagt
      901 ttgccgatac aagtgatcac gactggtctg tcgtgtctcg cagttccgat ccgctcgctt
      961 gcagacttac gtgacattcg cgtcgatgcg gcattgctgg ctgacatcta cactcgttat
     1021 ggcggcgccg gttgtcatgc attcacgcgc gagacgctcg aagtcggcgc cgcgcgtgca
     1081 cacgcgcgct tctttgctcc cgctgacaac attccggaag atcccgcgac cggcagcgcg
     1141 tgcggcgcgt tgggcggtta tctcattcat catggcgcgt tgagtttgga gcctgaggat
     1201 ggccggtaca agttcgtgat cgaacagggc gattttatta accggcccag ccgtataaat
     1261 ctcaacgtca aggggcaaac gggaaacgtc gaggaagtga aggtcggtgg ttcggccgtg
     1321 ctcgtcgcgc gcggcgaggt gattacagaa ccgagcgcgg tagcgccggg tcaaccgcat
     1381 caatcaggca cgtaggtgaa cgtacccggc gctaccgcgc tcggttctgt gcatatgctc
     1441 gatctaatga tcatcggggc tggtccggct ggattgtctg cggcggaagc ggcggcgcag
     1501 gccggtctcg attacatcgt gattgagaaa ggcacgattg ccaacacgat tcgccagtat
     1561 cctgtcggtc gcgcgatgtt ctccacccca aacgaagtcg agatgcacgc cggcaccttg
     1621 cgtcacgtga gcgagaagcc gacgcgcgaa gagatgttgt ctcactacat ccacttcgtg
     1681 ctcgatctcg atctcaaaat caatactggc gaggaagttg tttcagtaaa cgggaacata
     1741 aacgagggtt tcgttgtcaa aacggaccgt ggcgagtacg aagcccgacg tgttttgttt
     1801 gcaatcggag cgatggagca tccgcgacga ctgaatgtgc ccggtgaaga gttgccgaag
     1861 gtgcatcatc ttttcgtcga gccttatgca tacgttcgca agaacgccct cgtcgtcggc
     1921 ggtggcaaca gcgcggcgga agcggccttg tttctggcag aggaaggcgc gcgcacgacg
     1981 atggccatct ggcgcgaaga ttgggagaac cgcgatccga aagccggcgc gatgaaacac
     2041 tgggtacgca cgccgctcga agcacaggtc gcagcaggac gactcaacgt cgtgctttac
     2101 aaaaacgtcg acgagattca tgaagatgat gtgacgttga cgactgagac cggcgagtcg
     2161 ctgacgattc cgaacgacgt ggtattcgtg ctcgttggca gtgatgctga tctgacgtta
     2221 ttgaaacaac tcggcgtgca gactcagccg gggaaactgg ctgaagttcc ggtctacgat
     2281 ccggaaactt ttgagacgaa cgtgcgtgga gtatacgtgg ccggtcactt cacgcatgct
     2341 cgacatatca aagctgcgat cgacgtgccg cgtcaaatag ttccactgat cgcgcatgaa
     2401 ttgaagagca gtcgttagac aggatttaca gtatgtttca ggattgacat ataaatcctg
     2461 aaacatcttg taaatcctgt ctaaaactac ctgcgcagga gaagcctgat gacccgtctc
     2521 gcgctactaa tctccctact catctgtctc gcctcgccaa tcaacgcaca aaagaaaacg
     2581 gtatcatccc tctgcacgcg cgataacgct ctcgacaccg ccaaacaaca gatcctcatc
     2641 acgcgaactt tcgataaccc ggtccatcgc atcaccgtcc tcgtgcgggc cgccgatctg
     2701 ctctggccac acgatcagga gaagtcgctc gccgcgttca tggaagcctt cgatttcgcc
     2761 gtccaaaact tcaaagaaaa aggcgatcag atccagcgca cctcgagcag ccggttcgca
     2821 gccgtaatcc ccgttcccga tcagcgcttc gtagtgctct cggcattagc aaaacgcgac
     2881 ccggcgcgcg cccgcaagct atcagagcag atgcttcgcg acgatgtctc cgaagctgac
     2941 aagcccgcaa ccgacgatca atcaaaacgc agcagcgccg gcaagctgtt aaccatggcc
     3001 ttcagtctcg ccgcgtctga tccaccatcg gcggttaact tcgcgcgcca gagttttcgt
     3061 tatccagcca ccatgcagtt acccatcttc ctgtacgagc tcgccaagtc gagcccgcag
     3121 ctcgcagatc aattttatgt ggaagcgcta aatgcctacg gcgccgcgcc gatggaccag
     3181 tttctttatc tctcgtcata tccgtttgcc aacaagcgcg acgccggcga gatgccgggc
     3241 tacactttct acgttattcc cgaaggcttc gaaccaaatc gatcgttgca acggcagttc
     3301 gttcggacct tgctcgcacg aatcgacggc gcgatggaga cacccgtgca ggaggcgcag
     3361 cgcttatcgg atcatgcaca gatgtggctc gctctcaacc gccttgaaaa acaggtgcag
     3421 tcaaacttac cggaattcac cgatgcgttc gcgcaatcaa aagacaagct gtttgctttg
     3481 ttgacgccga acacacaaag ggatgtcaac cagctcataa ccagcgacaa tacaccggcg
     3541 cgatcgtttg acgaacaggt tgaagcggcg cagaagcaaa caaacgtcgg gcggcgcgat
     3601 cagggtttgt catttgctgt gaccggctcg tcgaaagacg aaccggtgga caaggtgctc
     3661 agcgtgatcg acaagatctc ggaggcgagc attcgcgacg cgctgcttaa ctggttttat
     3721 tactttcgca gccaggcatt cattaaagag aagaacttct cggaagcgag aaagtttgcg
     3781 agcaaggtga cggagctgga ccagcgagct tatttataca cgcgtatcgc ggaggagtca
     3841 ctcaaggccg ctgaagatca gacggaagta cgcgagatgc tgaacgaggt ggcgagtacg
     3901 atcaacaaag cgccgaagac aatcgtctcc gcgcgcgcgt tgctcgcgct ggcctatcta
     3961 tatgcgaagg tggacatgaa ccgcggcgtt gaagagttgg gtaatgcggt cacgacgatt
     4021 aacacgttgg agtcgcccga cttctcgcgg cagtttgtga tgataaagat tgaaggaaag
     4081 acgttcggca gtttcgcgtc gtttgcaaca cccggcttca accccgagaa cgcatttcgc
     4141 gaaatgggca agctcgactt cgacggcagc ctcgcgcagg ctacaaactt cagcgacaaa
     4201 tcattgcgcg cgttaacaac cctcgcagtg atcgaaccat gcctcgcaac taaacctatc
     4261 agggcgccaa aaaccaaggc accctaacaa gctctgttac gtgccttttt gtggctaact
     4321 aatttcgcgg cgagcgacgc ccgtctgttg cgcagcggcc tcgacctcac gcgccaccgc
     4381 ctccgctact cgcttatcaa aaaccgacgg gacaatatat tcgggatgca gctcgctatc
     4441 gctgatgatc gccgcaatcg catatgaagc cgcgagcttc atctcttcat tgatccgcgc
     4501 tgcacgacac gcgagtgctc cacgaaagat ccccggaaag ctcagcacgt tattgatctg
     4561 attcggatag tctgatcgac ccgtcgccat gaccgcgaca taaggcgccg catcctccgg
     4621 catgatctcg ggcaccggat tcgccatcgc aaacacgatc ggcttctcag ccatgttctt
     4681 cacgtcttcc acgccgatca cacccggcac tgacaaaccg aaaaacacgt cggcgccctt
     4741 gatcacgtca tgaacagttc ccttctcgtc attcgggttc gtgttctgcg cgtaccagtc
     4801 tttcatccag ttcatgtttt ctctgcgccc gcgatagagc gctcccgtct gatcgcagcc
     4861 gacgatattt ttaacgcccg cggccatgat gatcttcgtg caagcaacac cggcagcacc
     4921 gactccattc acgatcactt tgacgtcgct catctgctta ccgacgagtt tgagcgcatt
     4981 gatcaatgcc gcgagcacga ccaccgcggt gccgtgctga tcgtcgtgaa agaccgggat
     5041 atcgagctct tccctgagtc gctcttcgat ctggaaacaa cgcggcgccg agatgtcttc
     5101 gagattgatg ccaccgaaca ccgtcgagat cgcttttaca gtgcgcacga tctcgtcggg
     5161 atcttttgta ttaagacaaa tcggaaacgc atcgaccccc gcgaattctt tgaacagcat
     5221 cgccttccct tccatcaccg gcaaggctgc ggctggtcca atgtcgccga gcccgagtac
     5281 tgctgttccg tcggtcacga tcgcgactgt gtttttcttg atcgtgaggg tgaaaacttt
     5341 ttccggatct ttggcgatcg cttcgcagat acgagcgacc ccgggagtgt aggccatcga
     5401 gagatcggcg cgagtcttta gcggcacttt cgagaccact tcaatcttgc cgccgaggtg
     5461 catcagaaac gtcggatcgg agacctgcag caactcgacg ccgtcgacat ctttaacagt
     5521 ttcaacgatc tgttcgccgt gtttgcttga gactgagctg acggtgatgt cgcgaatgat
     5581 gtagttattg ccgacggtga cgatatcgat ggcgccgatg tcgccgccgg cgcggccaat
     5641 agcggtagtc aattccccaa gcgatccggc ccgtgaagaa agcctgacgc gcaaggtcaa
     5701 actgtaactt gcgctgggtg ttggttgcat gtgatctcgg tccacagatt acaccgattt
     5761 aagaatctgt gtaatctgtg gatgttcttt cgtaagtttt aaggaccgct ggccgagtag
     5821 acgataaaga tccagcgcct ctgccagttc tttcttcttc aacgccgcga gatgtcgttt
     5881 gacagtttcc acatcgccgc gtgaaaacgt tcccgtcagc gattcttccg gcgatttagt
     5941 ttgcaaactt cggacggtgc tcgcaatcag cggcaacagg accgcgcgcg ctgtcttccg
     6001 gtcgagccca cactcgccga gcatctcgag cgccacatca aacaacgcca ccacgttacc
     6061 cgaaaccatc accgccgcag catggtaaag cggcttatcc tcggttcgga tcgaaaaact
     6121 cttaccaccg aggacgcgca cgatctgctt ccccaaacgc agggcgctcg gatccccctc
     6181 aacgctccaa aacgctccct ccaaatgagc atcatgaacc gcactaaccg acatcagcgg
     6241 atgaatagac ccggtatgcc aacccttttc tcgcaacggc gccagcacct cactcgatag
     6301 cgcgccgctt gtgtgcagtg ccgtcctcct cttcttctga tccgcgttaa atcgaacatt
     6361 gcttaattcg tccgctactc gtggaatttg atcgtcgggt acagtaatca gaaaaagatc
     6421 cgcgtcgatc agcgtccgca attgttttgc agccaatacc tgcacggctg cgtctaaaag
     6481 gcgcgccgct ttgcgcgcac tctgcccgcc gcgcgacacc agggaacgga tagtgtagcc
     6541 acttcctgaa agggctagtg cgagggtagt cccgagtcga ccggtaccga tgatcgagac
     6601 ctccgacttt tgcttccttt tcgcgtccgg cataggtata ttgagggcgt taaggcgcga
     6661 agaaacctaa tcctcctggc caggaagtta tgatcggcca cacaaccaag tcgccgaccc
     6721 tgagcatcat agactgcgcg tctggattta gcgaaagaac gtcagcgacc cctgccctca
     6781 aggtcattcg aaatttttgg aggtttttga gatgaacaac ttctcccgcg ccacgagtgc
     6841 ttttgtttta accgcatcgc tattattcag ttccgtggcc gcggtcgcgc aaacgacagc
     6901 caccaagccg gccgacaacg cgcagacaac cagccagacg caatccgatc agaaaactca
     6961 gaccgacgcg aaagccgagg cacaaaaagc cacaccggcg ccggtcgctg ttaagaccag
     7021 caacaagccg ctttcgacta acgaaaatcc tgagatgatc ggcaagcgga acatcaacac
     7081 cggcatcatc gcgaagatga gcggttcgac cgagaaggaa gtgcggcagg gacgtgaggc
     7141 ggcagcggaa gtcgaccgcc aggctaagtt tgttgacgac ccgctgatca ccgagtatgt
     7201 gaaccgcgtc ggtcagaaca tcgtgctgca ctcggacgcg aaggtcccgt tcacgatcaa
     7261 ggtcatcgat tctgatgaag taaacgcgtt tgcccttccg ggtggtttct tctacgtaaa
     7321 caaaggactc attctcgcgg ctgataacga agccgagctc gcgggcgtga tggcccacga
     7381 gatcgcacac gttgccgcac ggcacgcggt cgagaaccag acgaaggcca gcctgctcga
     7441 gtatgcggcc ctcggcggct caatcttcct gggaggtatc cccgggatga tttaccagaa
     7501 cacggccggt atcggcctgc tcggaatctt catgaagttc agccgcagcg cggaagagga
     7561 agcagacaag ctgggcgttc agtacatgta cgctgccggt tacgacccgg gcgcaatggc
     7621 gacgatgttt gagaagctcg aagccaagaa taagaagaag cccggattcg tcgcgcgcgc
     7681 tttctcgacg cacccggccc cgccggatcg gagagcgtcg gcgctggcgt tggcagcgcg
     7741 gttcccggaa cacgaggaat atgtgatcag cagttccgag tttcagcgcg tgaagggccg
     7801 gttgttaaga ctttcaaatg cgcgtgcctc gacggctgga gcaattcaag caggtgatga
     7861 cagcggcgca cccggacgtc cgactctgaa gcgtcgtcaa ccgacaccgg aagaaacagg
     7921 cacgacaccg gacggcgagc agcagcaaaa agatgcgccg ccgaagttga agaaagaccc
     7981 gagcgccaca aaaactcccg acaagccgta atcgtttgtg gtaacttaac tgctgaggcc
     8041 cttcggcatc ccggagggcc ttttcatgtc agagcttgtt tatccacaga ttacgcagat
     8101 tgaattgaaa tctgtgtaat ccgtgtaatc tgtggctaag tcggttgccg cgggttgaca
     8161 atattttggg ccgttcggta ttcttaccgt tcgcgtttta ttcgcttaaa acagagatag
     8221 ttaactatgt cgacttactt ccccagcgga aaggaactcg ctggaagacg aaaatggtac
     8281 gtggtggatg cgagcggtca aaccgtcggg catctggcct ctgaggttgc ttcaattctg
     8341 tctggcaagc gtagccctga gtggacgccg tttatggata tgggcgatca cgtcgttgtg
     8401 atcaatgcct ccaaagcggt cctgaagggc aacaaggttg agcagaagct ttatcgtcat
     8461 cacactcgct atccgggagg tctgagaacg accacggcga aagagatgat tgccaagcgc
     8521 cctgagcgag ttatcgagct cgccatcaag ggcatgctgc cgaagaccaa gctcggcaag
     8581 gcgatggcga aaaagctcaa gatttacgcc gatgccgagc acccgcactc agcccagcgc
     8641 ccgcaagcct acgacctgac tcacaacacc aactcataac agttttagta aaagatagag
     8701 gaaattactg tggctgaaat tcagtattac ggaacggggc ggcgcaaatc ggcaacggcc
     8761 cgagtttacc tgcgacccgg cggcggcgaa gtcaaggtca accacaaacc tttcgatcgc
     8821 tacttcccga acgaaacgtt gcggatgatc attcgtcagc ctttgcaact gactgaaacc
     8881 acaaacaaat ttgacctcgt catcaacgtt gctggtggtg gcccggcggg ccaggcgggc
     8941 gcgatccggc acggcatcac gcgagcattg ctcgagttca atgccgacct tcgtccagcc
     9001 ctcaaacatg caggtctggt gacccgcgat ccgaggatca aggaacgcaa gaagtacgga
     9061 caaaagggcg cgcggaaacg tttccagttt tcgaagcgat aattcgatca atccattgcc
     9121 g
//
